LOCUS       DALAAR010000001        99573 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000001 DALAAR010000000
VERSION     DALAAR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 99573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.001"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..70)
                     /locus_tag="O2458_000001"
     CDS             complement(<1..70)
                     /locus_tag="O2458_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCZ3885894.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKM"
     gene            221..1219
                     /locus_tag="O2458_000002"
     CDS             221..1219
                     /locus_tag="O2458_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ3885895.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1451..2332
                     /gene="rbsU"
                     /locus_tag="O2458_000003"
     CDS             1451..2332
                     /gene="rbsU"
                     /locus_tag="O2458_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCZ3885896.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2347..2751
                     /gene="rbsD"
                     /locus_tag="O2458_000004"
     CDS             2347..2751
                     /gene="rbsD"
                     /locus_tag="O2458_000004"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCZ3885897.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2779..3693
                     /gene="rbsK"
                     /locus_tag="O2458_000005"
     CDS             2779..3693
                     /gene="rbsK"
                     /locus_tag="O2458_000005"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCZ3885898.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3791..3925)
                     /locus_tag="O2458_000006"
     CDS             complement(3791..3925)
                     /locus_tag="O2458_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885899.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3944..4705
                     /locus_tag="O2458_000007"
     CDS             3944..4705
                     /locus_tag="O2458_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3885900.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5139..6575)
                     /locus_tag="O2458_000008"
     CDS             complement(5139..6575)
                     /locus_tag="O2458_000008"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCZ3885901.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6591..7382)
                     /locus_tag="O2458_000009"
     CDS             complement(6591..7382)
                     /locus_tag="O2458_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ3885902.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7531..8235
                     /locus_tag="O2458_000010"
     CDS             7531..8235
                     /locus_tag="O2458_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3885903.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8343..9044)
                     /gene="lrgB"
                     /locus_tag="O2458_000011"
     CDS             complement(8343..9044)
                     /gene="lrgB"
                     /locus_tag="O2458_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCZ3885904.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9037..9480)
                     /gene="lrgA"
                     /locus_tag="O2458_000012"
     CDS             complement(9037..9480)
                     /gene="lrgA"
                     /locus_tag="O2458_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCZ3885905.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9593..10333)
                     /locus_tag="O2458_000013"
     CDS             complement(9593..10333)
                     /locus_tag="O2458_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCZ3885906.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10336..12090)
                     /locus_tag="O2458_000014"
     CDS             complement(10336..12090)
                     /locus_tag="O2458_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ3885907.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12337..13011)
                     /gene="scdA"
                     /locus_tag="O2458_000015"
     CDS             complement(12337..13011)
                     /gene="scdA"
                     /locus_tag="O2458_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCZ3885908.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13151..14875)
                     /gene="tarS"
                     /locus_tag="O2458_000016"
     CDS             complement(13151..14875)
                     /gene="tarS"
                     /locus_tag="O2458_000016"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ3885909.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(14908..16596)
                     /locus_tag="O2458_000017"
     CDS             complement(14908..16596)
                     /locus_tag="O2458_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ3885910.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16618..17643)
                     /locus_tag="O2458_000018"
     CDS             complement(16618..17643)
                     /locus_tag="O2458_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ3885911.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17636..18352)
                     /locus_tag="O2458_000019"
     CDS             complement(17636..18352)
                     /locus_tag="O2458_000019"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCZ3885912.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(18628..19797)
                     /locus_tag="O2458_000020"
     CDS             complement(18628..19797)
                     /locus_tag="O2458_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCZ3885913.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(20225..21919)
                     /locus_tag="O2458_000021"
     CDS             complement(20225..21919)
                     /locus_tag="O2458_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ3885914.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(21941..22966)
                     /locus_tag="O2458_000022"
     CDS             complement(21941..22966)
                     /locus_tag="O2458_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ3885915.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(22959..23675)
                     /locus_tag="O2458_000023"
     CDS             complement(22959..23675)
                     /locus_tag="O2458_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCZ3885916.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(24203..25247)
                     /locus_tag="O2458_000024"
                     /pseudo
     CDS             complement(24203..25247)
                     /locus_tag="O2458_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(25271..25417)
                     /locus_tag="O2458_000025"
     CDS             complement(25271..25417)
                     /locus_tag="O2458_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885917.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(25419..26474)
                     /locus_tag="O2458_000026"
     CDS             complement(25419..26474)
                     /locus_tag="O2458_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCZ3885918.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(26492..27751)
                     /locus_tag="O2458_000027"
     CDS             complement(26492..27751)
                     /locus_tag="O2458_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCZ3885919.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(27978..28256)
                     /locus_tag="O2458_000028"
     CDS             complement(27978..28256)
                     /locus_tag="O2458_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ3885920.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(28279..28746)
                     /locus_tag="O2458_000029"
     CDS             complement(28279..28746)
                     /locus_tag="O2458_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ3885921.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(28731..30827)
                     /locus_tag="O2458_000030"
     CDS             complement(28731..30827)
                     /locus_tag="O2458_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ3885922.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(30948..31055)
                     /locus_tag="O2458_000031"
     CDS             complement(30948..31055)
                     /locus_tag="O2458_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885923.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKYRYN"
     gene            complement(31165..32100)
                     /locus_tag="O2458_000032"
     CDS             complement(31165..32100)
                     /locus_tag="O2458_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ3885924.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            32462..33991
                     /locus_tag="O2458_000033"
     CDS             32462..33991
                     /locus_tag="O2458_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ3885925.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(34311..35264)
                     /locus_tag="O2458_000034"
     CDS             complement(34311..35264)
                     /locus_tag="O2458_000034"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ3885926.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(35575..35682)
                     /locus_tag="O2458_000035"
     CDS             complement(35575..35682)
                     /locus_tag="O2458_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885927.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            35837..36982
                     /locus_tag="O2458_000036"
     CDS             35837..36982
                     /locus_tag="O2458_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCZ3885928.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            37008..37181
                     /locus_tag="O2458_000037"
     CDS             37008..37181
                     /locus_tag="O2458_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885929.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            37338..37694
                     /locus_tag="O2458_000038"
     CDS             37338..37694
                     /locus_tag="O2458_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCZ3885930.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            37892..39367
                     /locus_tag="O2458_000039"
     CDS             37892..39367
                     /locus_tag="O2458_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3885931.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(39681..40823)
                     /locus_tag="O2458_000040"
     CDS             complement(39681..40823)
                     /locus_tag="O2458_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCZ3885932.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            41264..42841
                     /locus_tag="O2458_000041"
     CDS             41264..42841
                     /locus_tag="O2458_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCZ3885933.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            42867..44372
                     /locus_tag="O2458_000042"
     CDS             42867..44372
                     /locus_tag="O2458_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCZ3885934.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            44484..45695
                     /locus_tag="O2458_000043"
     CDS             44484..45695
                     /locus_tag="O2458_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCZ3885935.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            45881..48142
                     /locus_tag="O2458_000044"
     CDS             45881..48142
                     /locus_tag="O2458_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCZ3885936.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            48172..49356
                     /locus_tag="O2458_000045"
     CDS             48172..49356
                     /locus_tag="O2458_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ3885937.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            49670..49777
                     /locus_tag="O2458_000046"
     CDS             49670..49777
                     /locus_tag="O2458_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885938.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(49959..51788)
                     /gene="coa"
                     /locus_tag="O2458_000047"
     CDS             complement(49959..51788)
                     /gene="coa"
                     /locus_tag="O2458_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCZ3885939.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            51978..52322
                     /locus_tag="O2458_000048"
     CDS             51978..52322
                     /locus_tag="O2458_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCZ3885940.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(52486..54249)
                     /locus_tag="O2458_000049"
     CDS             complement(52486..54249)
                     /locus_tag="O2458_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCZ3885941.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(54585..55340)
                     /gene="pflA"
                     /locus_tag="O2458_000050"
     CDS             complement(54585..55340)
                     /gene="pflA"
                     /locus_tag="O2458_000050"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCZ3885942.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(55363..57612)
                     /gene="pflB"
                     /locus_tag="O2458_000051"
     CDS             complement(55363..57612)
                     /gene="pflB"
                     /locus_tag="O2458_000051"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCZ3885943.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            58200..59168
                     /locus_tag="O2458_000052"
     CDS             58200..59168
                     /locus_tag="O2458_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ3885944.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            59165..60721
                     /locus_tag="O2458_000053"
     CDS             59165..60721
                     /locus_tag="O2458_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ3885945.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            60714..61472
                     /locus_tag="O2458_000054"
     CDS             60714..61472
                     /locus_tag="O2458_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3885946.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(61831..63210)
                     /gene="uhpT"
                     /locus_tag="O2458_000055"
     CDS             complement(61831..63210)
                     /gene="uhpT"
                     /locus_tag="O2458_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCZ3885947.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            63443..63937
                     /locus_tag="O2458_000056"
     CDS             63443..63937
                     /locus_tag="O2458_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCZ3885948.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(64299..65267)
                     /locus_tag="O2458_000057"
     CDS             complement(64299..65267)
                     /locus_tag="O2458_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCZ3885949.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(65322..66362)
                     /locus_tag="O2458_000058"
     CDS             complement(65322..66362)
                     /locus_tag="O2458_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ3885950.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(66387..67463)
                     /locus_tag="O2458_000059"
     CDS             complement(66387..67463)
                     /locus_tag="O2458_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ3885951.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(67537..68376)
                     /locus_tag="O2458_000060"
     CDS             complement(67537..68376)
                     /locus_tag="O2458_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ3885952.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(68378..69646)
                     /locus_tag="O2458_000061"
     CDS             complement(68378..69646)
                     /locus_tag="O2458_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ3885953.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(69649..70920)
                     /locus_tag="O2458_000062"
     CDS             complement(69649..70920)
                     /locus_tag="O2458_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ3885954.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(70933..72030)
                     /gene="ugpC"
                     /locus_tag="O2458_000063"
     CDS             complement(70933..72030)
                     /gene="ugpC"
                     /locus_tag="O2458_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCZ3885955.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(72413..72991)
                     /locus_tag="O2458_000064"
     CDS             complement(72413..72991)
                     /locus_tag="O2458_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ3885956.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYDIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            73200..73826
                     /locus_tag="O2458_000065"
     CDS             73200..73826
                     /locus_tag="O2458_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCZ3885957.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            74016..74789
                     /locus_tag="O2458_000066"
     CDS             74016..74789
                     /locus_tag="O2458_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3885958.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(75087..77093)
                     /gene="ggt"
                     /locus_tag="O2458_000067"
     CDS             complement(75087..77093)
                     /gene="ggt"
                     /locus_tag="O2458_000067"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCZ3885959.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(77131..78906)
                     /locus_tag="O2458_000068"
     CDS             complement(77131..78906)
                     /locus_tag="O2458_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3885960.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(78923..80086)
                     /locus_tag="O2458_000069"
     CDS             complement(78923..80086)
                     /locus_tag="O2458_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3885961.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(80092..81570)
                     /locus_tag="O2458_000070"
     CDS             complement(80092..81570)
                     /locus_tag="O2458_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3885962.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            81527..83119
                     /locus_tag="O2458_000071"
     CDS             81527..83119
                     /locus_tag="O2458_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3885963.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(83384..84496)
                     /locus_tag="O2458_000072"
     CDS             complement(83384..84496)
                     /locus_tag="O2458_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCZ3885964.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(84483..85406)
                     /locus_tag="O2458_000073"
     CDS             complement(84483..85406)
                     /locus_tag="O2458_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCZ3885965.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(85408..86250)
                     /locus_tag="O2458_000074"
     CDS             complement(85408..86250)
                     /locus_tag="O2458_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCZ3885966.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(86455..89244)
                     /locus_tag="O2458_000075"
     CDS             complement(86455..89244)
                     /locus_tag="O2458_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCZ3885967.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            89457..89738
                     /locus_tag="O2458_000076"
     CDS             89457..89738
                     /locus_tag="O2458_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885968.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(89932..90807)
                     /locus_tag="O2458_000077"
     CDS             complement(89932..90807)
                     /locus_tag="O2458_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ3885969.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(90810..92264)
                     /locus_tag="O2458_000078"
     CDS             complement(90810..92264)
                     /locus_tag="O2458_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ3885970.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(92276..93172)
                     /gene="murQ"
                     /locus_tag="O2458_000079"
     CDS             complement(92276..93172)
                     /gene="murQ"
                     /locus_tag="O2458_000079"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCZ3885971.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(93172..94227)
                     /locus_tag="O2458_000080"
     CDS             complement(93172..94227)
                     /locus_tag="O2458_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCZ3885972.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            94813..96858
                     /locus_tag="O2458_000081"
     CDS             94813..96858
                     /locus_tag="O2458_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ3885973.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(96837..96938)
                     /locus_tag="O2458_000082"
     CDS             complement(96837..96938)
                     /locus_tag="O2458_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3885974.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            97132..98772
                     /locus_tag="O2458_000083"
     CDS             97132..98772
                     /locus_tag="O2458_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCZ3885975.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            98839..99396
                     /locus_tag="O2458_000084"
     CDS             98839..99396
                     /locus_tag="O2458_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCZ3885976.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
BASE COUNT        31822 a        18591 c        15523 g        33637 t
ORIGIN      
        1 acattttaaa attcttgtta tcataatcat taaaaggtat taaccttaat aatatattct
       61 ctcgtctcaa ccttaatcgt atacttcaga cgtctgtttg tagacaataa aagtcattca
      121 cgtcttcata tgtcatcaaa tgtttatcat gatatgatga atataataat cgggtatata
      181 actgtatgat taattacaca ataaagagaa ggaatataat atgaaaaaag tgtcaattaa
      241 agatgttgct agagaagctg gtgtatcagt tacaactgtg tcacatattt taaatcataa
      301 tgatagtcgt ttttccgcaa caacgataaa aaacgtacat gctgtttcag aacgtttagg
      361 ctatgcccct aataaacatg caaaacaatt gcgcggcagt aaaattcaaa ctattggcgt
      421 cattttgcct agcttaacaa atccgttttt ctcagcactg atgcaaagta ttcatgacca
      481 taaaccatct gatgttgatt tatgcttttt aacatctaca gcaactgatt tgtatgacaa
      541 tattaaacat ttaattgatc gaggtattga cggattaatt atcgcacaat acatatcatc
      601 cccggacgcc ctaaataact atctaaagaa acatcatgta ccttatgtcg tactggatca
      661 aaatgaccat caaggctata cagattttgt tcggacaaat gaatatcaag gtggacaact
      721 tgcagcacaa catttaatag aactcggtca caaccatatg ataattgttg caccatatga
      781 catgatggcg aatatgtcga ctcgtgtcgc tggatttgtc gatactttgc gcgcgaatca
      841 attgccagaa ccacaaatcg tccatactga attatctaag cgcggtgggc taaccattgt
      901 tgatgacatc atggttcaat ctgccactgc aatcttcgct attaacgatg aactcgctat
      961 tggcatttta cgaggactaa ttgaacatgg catcagtatc ccgaaagata tctcattaat
     1021 aggttatgac gacattgatt atgcagcgta cgtctcgcca cctttaacta ctgtggcaca
     1081 acctataact gatattggca aaacatcttt aaccttatta cttcaacgat tacagcactt
     1141 agataaatcc attgatatga ttgaattacc aacgacttta aaaattcgtg caacaactgg
     1201 ctatcatctt tcaaactaac tacgtatctt ccgaaatata ctcatcattg ttaggccctt
     1261 agcgttgctt taatgctgag ggtttttaat cataattatt ttactaagaa attaaaataa
     1321 taatgtatga atttttaaat atgatttaaa cgttttcagt ttttatgaaa acgcatgcat
     1381 tttacaaata aaaatggtac gatggcactg gtaaaacgtt ttactaaaaa caaatcatga
     1441 ggtgtataac atgagcattg ttgcattact tatcgggtta ggccccttaa ttggctgggg
     1501 cttcttccca acagtcgctt caaagtttgg tggtaaacct gtacatcaaa ttatcggtgc
     1561 tactgtaggt acgttaatct tcgctattat attagccgta gtcacatcaa gtggcttacc
     1621 tactggaacc aatttgctat tcgccttatt atcaggtgca ggatggggat tcggacaaat
     1681 cattacattt aaagcgttcg aattagtcgg ctcatctcgt gccatgccag tcacaacagc
     1741 attccaatta ttaggcgcat ctttatgggg tgtctttgca ttaggaaatt ggccaggcat
     1801 tggtcataaa atcattggat ttacagcttt agtcgttatt ctaattggag cgcgtatgac
     1861 agtttggagt gaacgcaaag aagcaagtaa cgccaaaaat ttacgtcgtg cagtggtact
     1921 tctgttaatt ggtgaatttg gatactggtt atattcagct gcaccgcaag caacttctat
     1981 tgatggccta actgcctttt tacctcaagc aatgggtatg gtaattgttg cagtcattta
     2041 tggctttatg aatatgaaag cagagaatcc attccgtaat aaaattacgt ggttacaaat
     2101 tatttcaggt ttcttctttg catttggtgc tttaacatat cttatttcag cacaacctaa
     2161 tatgaatggt ttagcaactg gatttattct ttctcaaaca tccgttgtgc ttgctacatt
     2221 aactggtatt tatttcttaa aacaacataa aacgtcaaaa gaaatggtta ttacaatcat
     2281 cggcttagta ctcattttag tagccgcttc tgttacagta tttataaaat aaggagtgta
     2341 gatgtcatga aaaaatcagc tattttaaat gaacatattt caaaagcaat cgcgacaatt
     2401 ggtcattttg atttattaac gattaatgac gctggcatgc caattccaaa tgatcatcgt
     2461 cgtatcgacc tagctgtaac taaaaactta ccacgcttta ttgatgtctt agctacagtg
     2521 ttagaagaaa tggaaatcca aaaaatatac ttagcagaag aaataaaaga acataaccct
     2581 acacaattgc aacaaattaa acaattgatt tcatcggaaa tcgaaatcat tttcattcct
     2641 cacgaagaaa tgaaaagtaa cttagctcac ccattaaata aaggtaatat tcgtactggt
     2701 gaaacaacgc cctactctaa tattgcatta gaatcgaatg ttacttttta aaagttataa
     2761 cttgaaagga gcgtacacat gaccaacaaa gttgttattt taggttcaac gaatgtcgat
     2821 caatttttaa cagttgaaag atatgcacaa ccaggcgaaa cattacatgt tgaagaagca
     2881 caaaaagcat tcggcggagg taaaggtgcc aaccaggcta ttgccactgc acgcatgcaa
     2941 gcagacacaa catttattac taaaattggc actgatggcg ttgctgattt catcttagaa
     3001 gattttaaag tagctcatat tgatacatca tatattatca aaacagctga agcaaaaacg
     3061 ggccaagcct ttatcactgt gaatgcagaa ggacaaaaca ccatctatgt ttatggtggt
     3121 gcgaatatga cgatgacacc tgaagatgtt attaacgcaa aagacgctat aatcaatgca
     3181 gactttgtcg ttgcacaatt agaagtaccc atcccggcta ttatatctgc atttgaaatt
     3241 gccaaggcac atggtgtgac gacagtatta aatcctgcac cagcgaaagc attacctaat
     3301 gaattattat cattaatcga tattattgtg ccaaacgaaa cagaagccga attgttatct
     3361 gggattaaag taactaatga acaatctatg aaagacaatg ccaattactt tttatctata
     3421 ggcattaaga ctgttttgat tacgctaggt aagcaaggta catattttgc tactaaaaat
     3481 caaagccaac acatcgaagc ttataaagta aatgcgattg atacaactgc tgcaggcgac
     3541 acatttattg gtgcatttgt cagtcgctta aacaagtcgc aagataactt agcagatgct
     3601 attgattttg gtaataaagc gagctcactc actgtacaaa aacacggcgc gcaagcatct
     3661 attcctctac tagaagaagt aaatcaagtt taaatgaatc aaacacagct atgatatgaa
     3721 ggtttagcat ataacatgca acattcgtat atcatggctg tgctttttta tctttataaa
     3781 acatcatcta ttagaaataa ttttccacta aacctatgct tgttgactca tgtttagtta
     3841 taaatgaagt gacaattttt tgtaatcttt ttaacttcca aattatcgca tataaatatg
     3901 ctatattaat gataataatt atcaattaaa aggaggttat gctatgtcta aagaagctgg
     3961 tcatacattt ttagctaaat taggaaaaac tcgtctacgc cccggtggta aagaagcaac
     4021 agattggtta atacaacaag gggcattttc acaagataaa caagtgttag aagtggcatg
     4081 taatatgtgc acaacatcta tttatctagc tcatacatat ggctgtcaca ttcaaggcgt
     4141 tgatataaat aagaaagcat tagaaaaagc acaggaaaac atttcagcag caggtcttga
     4201 atcatatatt caagttcaac aagcgaatgc tgttaaattg ccctttgatg acaatcaatt
     4261 cgatatcgtt ttaaatgaag caatgttaac aatgttaccc atcgccataa aggaaaaagc
     4321 attacgcgag tactaccgag tcttaaagcc tgggggtatc ttgttaacac atgatattgt
     4381 catcgttaat gaatcacatg ccacacatgt tgttaaatca ttatctgcag caattaatgt
     4441 caatgtctca ccgcagacga aacttggctg gttagattta tataatcaag ctggttttaa
     4501 tcatgtgcat tatcatactg gtccaatgag tttaatgaca ccaaaaggtt taatttatga
     4561 cgaaggtatt gttggaacta taaagattat caacaatgct ttgaaaaaag aaaatcgacc
     4621 aatgttttgt aaaatgttta aaacgatgac taaattgcgt aaagatatga attatattac
     4681 ttttgtcgct aaaaaagagc actaaatata atgccactaa ctgtactttg tatctatgtt
     4741 tgactatcac tttaatttct ttgtgacact aatcatctac ttaacaatat cgttatcgtt
     4801 gattagtaag tcatcaattt tggttaaaga ctttcataaa cactcaaaca ttaacactat
     4861 acatagttag tggcattatt tttttcttaa aattttaaca tcacgggatt ggaacagaaa
     4921 tgatattttc gcaaaattta tttctgtcct tccccaactt gcattacctg tagaatttct
     4981 tttcgaattt ctctgtgttg gggccccgcc aacttgcatt acctgtagaa tttcttttcg
     5041 aaattctttg tgttgggggc ccgccattat aacactgtag gtcctactat attaatttat
     5101 gtctcgggct ctaaccctaa aatacttata atataccttt ataatttatc tccatttgat
     5161 gctatcactt gttgatacca atagaacgaa tcttttttca agcgttccat cgtgccatga
     5221 ccatcattat ctcgatcaac atatattaaa ccatagcgtt tcttcatttc accggttgta
     5281 aatgaaacaa tatcaatgat tccccacggt gtataaccga ttaaatctac accatcttga
     5341 tcaactgctt ctatcgctgc tgtaatatgt gcttttaaat attcaatgcg ataatcatca
     5401 tgaatatgtc catctaccac ttcatcaact gcaccaaaac cattttccac aataaaaagt
     5461 ggtaactgat aacgatcata taacacattc aatgtatatc ttaagccatc tggatcaatc
     5521 gcccaacccc aatcactcgt tgcgatatgc ggattctcca cagaatgatt caaaccaccg
     5581 ttgacgatgt tgttttcaac tgtagtatca acatcatgtt ttacagccgt agacatgtaa
     5641 taactaaagc caatataatc aaccgtgccc tgctgtaaaa tactgtcgtc cccttcttgc
     5701 catccaacat catatccttt tcgtgccaac atttttttag catagcttgg ataataacct
     5761 ctcacttgga catccgggaa aaagaaacgt aagcgattcg caatttgtgc ttccatcata
     5821 tctttcggat gacacgaata tggataaatg ggtacatgtg aaatcattgt tccaatctta
     5881 aacttcggat taatctcttt accaagacga actgctaaag cactggctaa aagttcatga
     5941 tgtgctactt gatacaagac ttcttcagga ttatcatttt ctgtcaatgc taccccagaa
     6001 ttcgtccata aaaagatagg atttgatgtg tccatctgat tattaatttc attaaacgtc
     6061 atccaatatg taactttatc tttatatctt tcaaatacaa cacgcgcaaa atgcacaaaa
     6121 taatcgacaa cttctctatt tctaaatcca ccataatgtt tcgctaaatg aagtggcatc
     6181 tcaaaatgtg ataacgtcac aacaggttca ataccttgtg caattaattc atcaaagata
     6241 cgatcataaa aggcgagtcc ttcttcattt ggcacatctt catccccatt cggaaagata
     6301 cgtgtccacg caatcgacgt acgtaaacat ttcaatccca tttctttaaa caaggcaata
     6361 tcttccttat aacgatgata aaaatcaata ccttcatgat ttggataata gtgattggga
     6421 tctatagatt ctgtaatctg acgtgctttg ccatgtgcac cactcgtcat aacatcaatt
     6481 acacttaacc ctttaccacc tttatcatat ccaccttcaa attgatttgc ggcaagagcg
     6541 ccaccccaca tgaaattttg cggtaatttt gtcattatta caacacactc ctagttttta
     6601 aatattttaa aaataacatc ttcagtcgta atgatttcat tcattgtcag ttctactttt
     6661 cctaaatcgg cagagttgct aataacgaca ataacgtcag cattgtagcc ttgttgttgt
     6721 atatattgct ggtcgaattg caacaacgtt tgccctgctt caacatgatc attttgcttt
     6781 acaaagcact tgaacccttt accttccaag tcaactgtat tcaagccaat atgaatgact
     6841 atgtccacac cgtcttctga ttgaatacca actgcatgct tagttggtac aatcatagat
     6901 attaaaccat tgaacggtgc gattactttg gattcttcat gagctttgat tgctaagcct
     6961 tcacctacca ttttctctct aaaaattgaa tctcttactt ctttaagtaa aatacgacga
     7021 ccagcgattg gtgctttcac taaaattggt gttgttactt ttacttgagg tgtataactt
     7081 gtatcttgac tattaatatt gtcagtattc tcactctctt cgacattagc attcttaatt
     7141 aatcgctgta attgctgata cacttcatcg acatttccat ttaattttat gactaactgc
     7201 gtatcatttt ctgctacaga tgtcacatca actatttgtt tcacttcatt tgctgtagaa
     7261 ggaattgtat gttgcatatg aattgtaatg cacttcgtat catacgttgc atcaacaatg
     7321 ttttcgacac cacctacagc tgtaagaatg tcttgggctt gttctttata tttattgctc
     7381 ataaatatta ccccctcaac ttgatagtag aatgaaaatg tatttactta cctccaatta
     7441 taattgtacc ggttcaattt gtaaacgccg atacaattat aatattttgt gctataataa
     7501 ttacagacaa agtgaaacga ggacagaata ttgttaaagt atgaacatat tgctaagcaa
     7561 cttaatgcgt ttatacatca atctaatttc aaacccggtg ataaattgcc aagcgtgacg
     7621 caattaaaag aacgttatca agtaagtaag agtactatca ttaaagcatt aggcttattg
     7681 gaacaagatg gtttgatcta tcaagcacaa ggcagtggta tttatgtgag aaatattgct
     7741 gatgccaatc gtatcaacgt ctttaagact aatggtttct ctaaaagttt aggtgaacac
     7801 cgaatgacaa gtaaggtact tgtttttaag gagattgcaa cgccacctaa atctgtacaa
     7861 gatgagctcc aattaaatgc agatgatacc gtctactatt tagagcgatt aagattcgtg
     7921 gacgatgatg ttttatgtat cgaatattct tattatcata aagaaatcgt gaaatattta
     7981 aatgatgata ttgctaaggg ctctatcttc gactatttag aatcaaacat gaaacttcgt
     8041 attggttttt cagatatttt ctttaatgta gatcaactca cttcaagtga agcttcatta
     8101 ctacaattgt ctacaggtga accatgttta cgttaccacc agacttttta tacaatgact
     8161 ggcaaaccct ttgattcatc tgacatcgta tttcattatc gtcatgcaca gttttatatt
     8221 cctagtaaaa agtaataaat acataaaaac gtctatatcc cagttataaa ctggagtata
     8281 gacgtttttt tacgataata acaatggctc aaattgctat tatcttgctt aggtttttcg
     8341 ttttagaaga atattgctac aaagacaggc acaactgcta caacaactac accaactaac
     8401 actaaagcta tacttgccat tgattcttct acaggtccta attctttggc tggtgctaca
     8461 cctaatgtgt gaccacttgt tccaagtgct aatcctcggg caatagggtt agtaattcgg
     8521 aaaagcttta agaatttatt acctagggca taaataatga caccatttaa aataactgct
     8581 aatgatgtta attcttttat accaccgata ccagctgata ctggtaacgc aatcgctgta
     8641 gttgctgctt gaggtaacat tgataaaata acatcattgg caaattgtgc taacttcgca
     8701 aaagttaaaa taattaataa cgctacaact gtaccgatac caatacctcc gatgatacga
     8761 tgccaatgtt taacaagcac ttcacgcttt ttatataacg gaatcgcaaa acagattgtt
     8821 gccggttcta agaagaagta aataatgtct ccacctattt tgtaagtctt atacggaatg
     8881 cctgttaaat agaggaaggc cacaccaaat accatactga caaatagcgg tgcgaataag
     8941 aagaaacgat tagttttttc aaataatatg gtcgctaaga aaaatggtat aacggataac
     9001 agtattccga agtaaggtgt gtttagtgct aagtggttaa tcatgagctt gtgcctcctc
     9061 tattttgatc ttttttgtga ctttgtcacc tttagatctc gaagtaactt tcataataat
     9121 ttgtgtgaca tagccagtac aaataagtaa tagtattgtt gagacgatta ttagtccaat
     9181 gattaaaaat ggtgcttggc taatgacacc taaagagtta acaactgaga taccggctgg
     9241 tacgaagagt aagccaatgt tatttgttag tgtcgttcct actttttcga cttcgcctaa
     9301 cttaacagca ccagtacata ataatacaaa taataatact aaaccgatta ctgatgcagg
     9361 cataggaatt ggcataaatg attcaattat tttcgataca aagagtacta aagcaattac
     9421 aatgacttgg tgaaaaaagt gtgctggttt tgatgcgtct ttttgttgtt tcacgaccat
     9481 tgcctcctac gtttgattta actaaagtat agatggctca cttcgatttg cgtgattttt
     9541 agtccgaaat acaaaatatc ataggtaaaa tgcataaaaa aaaggattac tgttaaagta
     9601 atcctatcga cgctttaaaa tctttcataa atgaacgtcc aacttgcatc ttgacaccat
     9661 ttgtcaatat taccatataa gtatagttaa accattgttg cacttcttta atgtgtttcg
     9721 tgttaataat atatgaacga tgaatacgta taaaataagt gggattcaat cgtttttcat
     9781 aacgattcaa tggctctgtt gtttcgtatt tatgattcgt tgtatgtatg gttgtaatac
     9841 cattatgtgt gccaatccca ataatatttt gttgctttaa catgtgaatt ttatcgtcaa
     9901 tttcaacagg taagctttga tcaaaattcg ccgacatatc attcgcaatt gcacttgcgt
     9961 tattatcatc tttggcttta gtcgcacgca ctttattgac tgcttgttca atacgttttt
    10021 gaccaaacgg tttcaaaata tagtctgtcg catttaattc aaatgcctgt actgcgtatt
    10081 ggtcatgtgc agttgcaaaa ataatcgcag gtggctcttt catcttttga atcttagctc
    10141 ctaattcgat cccattttca tccattaaat tgacatctaa aaatataatg tcatattgat
    10201 tgatcagtag tgcttccaat gtttctttta cattttctgc ctcattaatt tcttcaaaac
    10261 caccaatttc atttaataaa tatgttaatt cattacgtgc taatggctca tcatctatga
    10321 ttaatgcttt catatttatt cctcctcttg tctttcataa ggaagtacac accaaaaagt
    10381 ggtaccgctc gatgtcgatt caaattgtaa tgctgcggat tttccaaata atccttttag
    10441 gcgtaagttt aaattttcta aagcactacc agttccagac tctgattcta cagatgtttc
    10501 tcccaacaaa tgcattttat ctttagaaat accctgacca ttatcttgta caataatacg
    10561 tacatgtgtt gcagtttctt taatcactga cacgtcaata tcgttacctt gctttcgatt
    10621 cgtaaacgca tgtttgatgg cattttcaac taaaatttga attaaaaatg gtggtacaag
    10681 cacatcgcga tatttgtctt caacattaat attgatatta aatcttcctg gataacgtgc
    10741 ttgttcgagt gataagtatg cacgcacttg acttaactct ttatctaaag taatcgtatg
    10801 ttgcttagag ccttgtaaat tcgctctgaa aaaataactc aattctaata gtaactctcg
    10861 tgccttttcg ctatttattc taactaaagc tgagatcgtg ttaattgaat tgaagaaaaa
    10921 atgtggactc acttgtgcct gtaatgactt aatctcagca tctttcaata acttactttg
    10981 cgtttcggct tcaccaagtt caatttggct actaaaaata tttgccaatc cttctgcaag
    11041 ttgacgttcc acaaaagtta aatcattagg gtttgtaaaa tacatcttca atgtaccgac
    11101 gatagaacca tgcatctcaa gtggtatcac gatagctgct ctaagcgggc aattcggatg
    11161 actacaacca atctcttctt tagtatgaac ttctttcaac tttcctgatt tcaatacatc
    11221 tttagacaga cttgttaata tttcatttgt tggtatgtga tgatcactac ctgcacctac
    11281 atgcgataag atttcatttt tgcttgtaat tgctacggca gatactttca ttaaattttt
    11341 aataatcatc gcaatttgct gtgccgattc tctattcaat ccttctttaa aatacggcaa
    11401 tgtctggttc atcaattgca gtacatcatg tgtttgaaca gccttcattt gctcctcttg
    11461 ctttaatgtt gaaatgataa tagacataaa aatcgccgta ccaacgctat taacaataat
    11521 cattggtagt gcaattaatg atatgaggtc aaccgcatat gctttgtcgt gggaaaatgt
    11581 taaaatgctc aacatttgaa tcatttccat aacaattcca atcatggcac ttttcgcaat
    11641 actcgggtaa cgcttgcgtc tttgagcttg taagccaaaa taaccagcaa ttataccaat
    11701 aaatatagat gagataagat aaacttgtgc atccgcccca cccatataca ctctgaaaat
    11761 acctgaaata acgccaacaa atagacctac aaaagggcca ccaactaatc ctgcgacacc
    11821 tatcgttaat acacgtgtgt tagctaaaga tacatcatca tctaaacgga agtacacact
    11881 tcctgacaaa ctatgttgat gatcgatgac gataccagtt aaattagaca ttaaggcaaa
    11941 caaactgaaa ataatacata attgccaacg tgctttccat gtacgtcgac gattcattaa
    12001 gtttttaaaa tatggaatat tcatcaacac ataggccaaa ataataatta aacctacacg
    12061 ctcaagtaat aacattgtta gcgatagcac gatagcacct cagtgaatta taattatgtt
    12121 tattttatca taatagtgca tgtaaaattc atgaataaat tattaaaaaa acacgctact
    12181 atttaattat ctataccatt aaattttcat atcaattttt atgctaagtt gaccattttt
    12241 cttttcaaat aatgctgttt atgtcactat tatgactttt atacatagcg aaaaactgct
    12301 ggacttattt ttgccaacag tttctaatcg cgttatttat gatacttttt taaataatac
    12361 gtgattctct aaatgtacgt gttcatgtgt taacacttca agtgctttta atctctgata
    12421 aacaagtcgc caagtaccac acgcttctat cggaggttca taactagatg ttaattcgct
    12481 catttttact aacaattctc ccgttgcaat gtggtctgaa actaaatcat ctatcacagt
    12541 attaatatcg tctactacct caccttgctc atatttaatg agttttggaa aatcgacatc
    12601 gtcttctttt tgcatatgct ctaacatgcc atttttaaat gtatcgtatg tttcttttaa
    12661 ctcaactaaa tatggatgat taggtccatg tactttcgat aacttcgtca cataaggtgt
    12721 taaattttta aattcttctc ttagaggttc atgatatgct gattgaatat attgaataag
    12781 tgatgaaaca tttaaaaatt taggatttaa cgaacctggt gtattcgttt gttcaacgtc
    12841 gttgagacgc tgtaataatt cgttcaaatc tacatttttc ttttctaagg ctgctgcttc
    12901 tatacttact tgtccgccac aacaaaaatc tattcccaca cttctaaaaa tatccgctgc
    12961 tttcggataa tcagttacta catctgctac tatgtcattt ttatttatca ttatagataa
    13021 cctcctcatt tccagtgtat acaatttaat gtatatgaaa atgaaaatca gggaaatgtc
    13081 ataatcatgt cataaacagc aaaataaagc cctcaaatca tgttggctca caatgatttg
    13141 agggcattta ttatttattt agtggataag tgatatgtgc agtgtttaac ttaacaatat
    13201 ttttgaagtc tcgcttttta atatctttgc taagtaagat ttgcacttcg ttttctgatg
    13261 tttggcttaa cattcccatc accatgtcgt cttttagtac gacactagcc ataccttctt
    13321 caaatacata accacgatcc agaccagtta aaatgatttt gtttttacct tttaacttca
    13381 atgagattga atccgttaat gtaatagctg taaagtataa cgaaatgtta ttaaagtctt
    13441 ttgtgaaata cggtgttaat ctataaaaat cattattcaa atgaattaat gcacttgttt
    13501 ctgcagtata tgcatattta ttacgttgat taccaacacg cacttcaaat acttccgtac
    13561 ctatttggac ttctaagaaa acatcccaaa ttttttcttt aggtaaatac gctttaatat
    13621 cttctaacat aatgttaaaa cgatattgtt tattcgcatt gacacttaaa ggtacataca
    13681 ttgtgtgctt cttgtttcgt gaatgtaatt taagtctgac atcataatgt ccttcgccaa
    13741 tcataaattc atttaacgtt agctctgtaa caattttatg attatcaaat ttaaatgctg
    13801 tcattttcac ttttggctta gcgatatcaa tgcctttaaa gtatggttcg ccttcattga
    13861 attgaatgtt taatttacca tctacaatat caaaacggta gtattgacct tgacggtaac
    13921 tttcctccac agttctatag ttgtctatat ctttcgctct cgcgtagtgc aacaatggtc
    13981 gtaatttact catcaccaat gcatctacac gttctggaac tgcttgtata aagtctccta
    14041 gagcattaat ccattgtggt tgatcttcta gtttaacttt aagtgagaag ccattcgtac
    14101 gagaaaaact aaaatgacga tttaaaaatt ctgctaagat ttgattttta tgggcttctt
    14161 ctaaatctgc atttaatatt tctacagcaa tcaatctcat gacttcataa aagtcttcag
    14221 gtgaaacata cgcactactc atatgttcac cttcacgctt tgtagcataa taatacgctt
    14281 tatcacttaa cacactgatt cgatttgcat ttaaatatgc tttcattgta aataattgat
    14341 cttctgcact ctttaattct tctggaaaat aaatgtcatt atcttttagt aatgctgttc
    14401 tatagatttt agtcgggctt aaagtataga taattcttga atttaacaaa gtaacttcag
    14461 gtgctgtttc tttaaacata gattgtggta caccacgacc attaacacct ttcattttaa
    14521 tcaataagac atctgagtga tgttcatcaa taaatgctgc tgcatccttt aaagtctctt
    14581 tgtttatata gtcatcggag tccacaaata atacaaattc accttctgct tgttttaacg
    14641 ccacatttct aggtttacct ggaccaccag aatttgtttc gagttgactt actttcaagt
    14701 tcaatttatt gcgatacttt tcaactattt gtaacgtttg atctgttgaa cagtcatcaa
    14761 ctacaaccac ttcaaattca gttttcggaa aatcttgttt cgcaaggcta ttaagtaatt
    14821 ctgttatata cttttctgaa ttgtatgttg gaactattac tgaaaatttc atcattatac
    14881 ctctcccact ttgactacta tataaactta gctaccaaat aaatttctga ctaaacgctc
    14941 acttgatcgg ccatcttgat atttaaaatg tttatctaag aatggaatga ctttttctcc
    15001 ttcataatct tcattgtcca aggcgtccat taatgcgtca aatgattgca caattttacc
    15061 tggaacaaat gattcatatg gttcataaaa atcacgcgtc gtaatataat cttctaaatc
    15121 aaatgcatag aaaatcattg gctttttaaa tactgcatat tcatatatta aagatgaata
    15181 gtcactaatt aataaatctg ttatgaacag tatatcatta acttctctaa agtcagaaac
    15241 gtcaacaaaa tattgtttat gtttgtctgc aatattaagt ctatttttca caaatggatg
    15301 cattttaaat aatacaaccg cgttattttt ttcgcaatat cttgctaaac gttcaaaatc
    15361 aattttgaaa aatgggtaat gtgctgtacc atgaccacta cctctaaatg ttggtgcgaa
    15421 aagaatgact ttcttacctt taataattgg taattcatct tccatctctt gtttgatctg
    15481 tgtcgcataa gcttcatcaa atagtacatc agtacgtgga acacctgtag gcactacatt
    15541 tttctcttta ataccaaatg cttcagcgta gaatggaata tcggtttcag atgatacata
    15601 agcttttgta tagctacgat gatttaatga atcaataaat ggtccaccct ttttaccagt
    15661 acgactaaag ccaactgttt taaaggcacc aacggcatgc catacttgaa taacttcttg
    15721 agaacgtcta aaacgcactg tataaatcaa tgggtgaaag tcatcaacaa agatgtagtc
    15781 tgccttccca agtaaatatg gcaatctaaa cttgtcgatg atgccacgtc tatctgtaat
    15841 attcgcttta aaaacagtgt gaatatcata ctttttatct aaattttgac gtaacatttc
    15901 gttatagatg tattcaaagt ttccagacat cgttggtcta gagtctgatg tgaacaacac
    15961 cgtattccct tttttcaagt ggaaaaattt cgtcgtatta aatatcgctt taaaaataaa
    16021 ttgtcttgta ttaaatgatt gtttgcggaa atacttacgt aattctttat atttacgaac
    16081 gatataaata cttttaactt ccggagtcgt tacaacaaca tcaaggacaa attcattaac
    16141 atcgctagaa atttcaggtg taacagtata aaccgttttc ttcgaaatgc cgccttttct
    16201 aaattctttt aggtaagtct gcaataagaa attgatttta ccattttgtg tttctaattc
    16261 gttgtattct tcttcttgtt ctggctttag attttgatat gcatcattaa tcacatctgg
    16321 gtttaactgt gcaatataat caagttcttg ctcattcact aataagtact tatcttcagg
    16381 taagtaataa ccattatcta agatagctac attgaaacga caaacgaatt gattcccatc
    16441 tattttgaca tcattcgcct tcattgtacg tgtctcagtt aaatttctta atacaaaatt
    16501 actatcttct aaatctaggt tttcactatg tccttcaacg aataactgaa cacgttccca
    16561 atagatttta tctatatata tcttactttt aaccaacgtt aattcatcct tttctattta
    16621 cataatccat tttaatactg ttttacccca agatgtagac aggtctgctt caaaagcttc
    16681 tgtaagatca ttaattgttg caatttcaaa ttcttgacct tttaacaacg ctaatttatc
    16741 tacaatatct gggtattgaa tgtataagtc tacaacatct tggaaatctt ttgaaccact
    16801 tcgactacta ccaatcaacg ttagtccttt ttccaatact agacgtgtat taacttctac
    16861 tgggaactca cttacaccta acagtgcaat gcttccttct ggtgaaatgt aatcgatcat
    16921 ttgatttatg gctgattgac taccgcgacc acccacacac tcaaatgcat gatcaaatgt
    16981 taagccttca ggtattttat taataaagaa gacatcatca acaaatgaga agtgactcaa
    17041 tttataatct gttttaccaa agacatatat tttagactct ggatataatt tacgtaataa
    17101 aatggctgta atgtaaccta agttaccatc accccaaata ccaaatgtat ttttatttga
    17161 aatagatttc ttttcaaaac gacgaatagc atgcaaactt actgttacaa gctctgtata
    17221 tgaaataata cttaaatcaa tatcatcagg tagtggtaca gctctatcat gatttagcaa
    17281 cacaaaatct tgcataaacc catcatgtcc acttgatctg aagtagctcg attttaaata
    17341 gttttcagca atgacatcgt ctttttctgt cggcgtattc ggtaccataa ctacttttgt
    17401 acctttatta aacacacctt tactgtcaaa tacgacctca ccaacacctt catgaattaa
    17461 agacattggc aatttctgag ataagacatt ctcatcacgg ctaccagtat aatatctttg
    17521 atcggcagca caaattgaca tatataaagg acgtacaatg acatagtcac tgtaaatatc
    17581 tacgttgtta tacgtaactt caaattgtct aggtgcaact aattgatata cttgattaat
    17641 catcggcaat accacctcga ataatagcat tcgctacttt taaatcgtaa ggtgttgtta
    17701 ctttaatgtt atataactca ccttttacaa gtcgaaccgg tttgtttgtt tctacaataa
    17761 tcttacaagc atcagataaa atactctttt gctcatcact caactgtgca tagctttctt
    17821 ttaataaatt aatattaaac gattgaggtg tttgaccttg gtacatttca ttacgcactg
    17881 gaattgcatc aatcgtttga ttatctttag atgtaacaat cgtatctata gcatcaatca
    17941 ctgtatctac tgcaccgtac tctaaagcag cttgaatatt ttctttaata atacgatgcg
    18001 ttaaaaatgg tctaactgca tcatgtgtca caatgacatc gtcatcgtta ataccatttg
    18061 ttgattcaat atgtttaacg atattcataa ttgtatcgtt acgatcgcta ccaccttgaa
    18121 tgacttcaat tctttcatca gaaattttga attttctaag tgtatctttc gtatgcgtca
    18181 tccattgttg tggcgtcgcg ataataattt tttcaaaatc attaattaaa ataaattttt
    18241 ctaatgtatg gattaaaatc ggtttgttgt ctaaatctaa aaattgttta ggtaaaggta
    18301 cgttacccat tcttgagcct atacctccag ctagaatacc agcgtatttc ataaaatact
    18361 tcctccattc aactatatct atatttaatt atttaaattt cgttgcattt tccaattgaa
    18421 aactcatttt aaaatcaaaa ctctaaatgt ctgtgtatta cttaaaatta tacatatttt
    18481 gcttatattt tagcatattt tgtttaaacc tatattacat tatatcagac gttttcatac
    18541 acaaataata acatacaagc aaacatttcg tttattattt atatcactta actaattaat
    18601 ttataatttt ttattgtttt taagttatca cttaaaaatc gtttggcaaa ttcgttgtga
    18661 cgcttgtcca tcttctaatg aacagaattt ttgataaaat accgttcgtg cttcaatata
    18721 ctcatttgca gtctcatcga tttgttttaa tgcatcaatg agtgctgttt gattttcaac
    18781 aattggacct ggcaactctt ttttataatc catgtaaaaa cctctaagct catcgccata
    18841 tttatctaag tcatatgcat agaaaatttg cggacgcttt aatacaccga agtcgaacat
    18901 gacagatgag tagtcggtaa ctaacgcatc gctgattaag tataaatccg aaatgtcttc
    18961 ataatctgaa acgtctttca caaaatcatc atgttcatca atacgtgtca caactaaata
    19021 atgcatgcgt aataaaataa cataatcatc atccagcgct tgacgcaaag cttctatatc
    19081 aaagttaaca ttaaattgat atgaaccttc tcgaatcgct tcatcgtcac gccaagttgg
    19141 cgcgtacata atcacttttt tatctaatgg aatatttaat cttgtcttaa taccattaat
    19201 atattcagta tcattgcgtt tatgtgataa tttatcattt cttggataac ctgtttccaa
    19261 aatcttatct cgactaacat gaaatgcatt ttgaaatatc gatgtcgaat atggattagg
    19321 tgacactaga taatcccacc gttggctttc ttttttaaag ccatcttggt aattttgagt
    19381 atttgttcct agcattttaa cgttactaat atccaaacca atctttttta atggcgtgcc
    19441 atgccatgtt tgtaagtacg tcgttcgcgg tgatttatat aaccaatctg gtgtacgtgt
    19501 gttaatcatc cacgctttcg ctcttggcat cgctaaaaac catttcattg aaaactttgt
    19561 aacatatggt acattgtgct gttggaatat gtgttcatat ccttttttca caccccatat
    19621 taattgggca tcgctatgtt cagttaagta ttcatataat gctttggggt tgtcgctgta
    19681 ttgtttacca tgaaagcttt caaaataaat tagattcttg tttggcaatt ttgatagtaa
    19741 tttaaaagtc gtatatatac tatgttctat caatttttta attgtatttt taatcatgtc
    19801 gtacctccga cgtgtttttg taattatatt aatatgtatg agcaagctca ttgtaaccat
    19861 gcctattata gcatttcatc ataaaataca tttaaccatt acacttgtcg ttaattatca
    19921 tacgaaatac atgattaatg taccacttta acataacaaa aaatcgttat ccattcataa
    19981 cgtatgtgtt tacacattta tgaattagat aacgattgga tcgattattt tattttacaa
    20041 aatgacaatt cagttggaag gtgattgctt ttgattgaat cgccttatgc atgaaaaatc
    20101 aaaaggttat tctcattgta tagtcctgct tctcatcatg acatgttgct cacttcattg
    20161 tcagaaccct tcttgaaaac tatgccttat gactcatttg catggcaagt aatatatgcc
    20221 aacattagcg tctaaacaaa tctttgacta aacgttcact tgagcgacca tcttgatatt
    20281 taaaatgttt atctaagaat ggcacaactt tttcaacctc ataatcttca ttgtccaaag
    20341 catccattaa tgcatcaaag gactgtacaa ttttacctgg aacaaatgat tcaaatggtt
    20401 catagaaatc acgcgtcgta atgtaatctt ctaagtcaaa tgcatagaaa atcatcggct
    20461 ttttaaatac tgcatattca tatattaaag atgaataatc actaatcaac aagtctgtaa
    20521 caaagagaat atcgttaact tcacgatgat ctgacacatc gataaagtat tgtctatgtt
    20581 cacgtgaaat attaagtcta ttttttacga acggatgcat tttgaataac acaactgcat
    20641 tatgcttctc gcagtatctt gctaaacgtt caaaatcaat tttaaaaaat ggataatgtg
    20701 ccgtaccgtg accattacct ctaaatgtcg gtgcgaatag aataactttc ttacctttta
    20761 taattggcaa ttcatcttcc atctcttgtt taatttgtgt tgcataagct tcatcaaata
    20821 gtacatcagt acgtggtaca cctgttggta caacattttc ttctctaatt ccaaatgctt
    20881 cagcataaaa tggaatatcg gtttctgatg aaacatatgc tttcgtgtaa ctacgatggt
    20941 ttaatgaatc gataaacgga ccaccttttt tacctgtacg actaaagcca actgttttaa
    21001 aggcaccaac agcatgccac acttgaataa tttcttgtga tggtctaaag cgaaccgtat
    21061 aaattaatgg atggaaatca tcaacaaaaa tataatcggc cttaccaagt aaatatggca
    21121 atctaaactt gtctctccat ttgcgtctat ccgtaatatt ctccttaaat accgttttaa
    21181 tatcataatc aaaatctact ttttggcgta gtaactcatc atatacatac ttgaaattcc
    21241 ctgataaatt cggacgcgaa tctgatgtga ataatattgt tttgcttctt ttaatatgta
    21301 gtaactttgt aatattaaaa atagctttaa ataagaaact tctactttca aatgaagctt
    21361 tatgaccttg tttatgaagc cagtgtgcac ttgtcgcaat gacccctgat ttctcttgag
    21421 gtaaggtgat ttcaatatca aatacaaatt cgttaacgtc acttgctatt tccggagtaa
    21481 tggtatagac cgtatgttgt gatacgccac ctttacggaa aactttagca tcatacgcta
    21541 ataaatagtt cacttttcca ttttgtgtag tcagctcatt gtattcttca atttgctctt
    21601 catttaatgc cgcataagct tgatcaataa tatttggatt aagttgtcca atatactcta
    21661 actggtcatg ataaacaaat aaatatttat ccattggtaa ataataccca tcgtctaata
    21721 ttgcgacgtt gaatctacat ataaatgtat taccgtctat tttcatttca tttgcttcta
    21781 atgtttttgt ttcggttaaa ttcctcagaa caaattgtcc tgatgttaaa tcgacacctt
    21841 cactatgtcc ttcaatatat aactgaacac gttcccagta tatgttatca atatgtattg
    21901 cttgtttcgt ttttgtcatt gttattcccc catcggttta ttaaatcgtc catttcaata
    21961 ctgtttttcc ccaagatgtc gataaatcca tttcaaacgc ttggacgata tcttgcatcg
    22021 tacatacatt aatttcatgt ccttttaata atgctaactt ttcaactatg tctgggtact
    22081 tacgatataa atcaacaact tgctcaaaat ctttagagcc gcttcgacta ctaccaatca
    22141 acgttaatcc tttttcaagt actaatcgtg tattcacttc cacgggtaat tcacttacgc
    22201 ctaacaaagc aatactgcct tctggtgaaa tatgttcaac tatttgttga agtgcaactt
    22261 gacttccttt acctccaaca cattcaaatg catgatcaat tttaagatca tctggtattt
    22321 gatttactgt aaagatgtca tctacaaatg aaaaatgact taatttatag tctgtcttac
    22381 caaatacata agttttagct tctgggtaca acttacgtag caaaatagca gtaatataac
    22441 ctaagttacc atcaccccaa ataccaaagc tggttttcaa aggtatagat ttacgttcaa
    22501 atcgttgtat agcatgataa cttactgaca ctaactctgt gtatgaaatc gtactcaaat
    22561 caatgtcatt aggcagcgga acgatacgat catgtgccat cacaacgtag tcttgcataa
    22621 aaccatcata accactagat ctaaaataac tagaggctaa gtaattctcc gcaataatat
    22681 gatgttgctc tgtaggtgta ttcggtacca ttactacttt cgtacctttt tcaaataccc
    22741 ctttactatc aaatacaact tcaccaacag cttcatgaac taatgacatt ggtaattttt
    22801 tgcgtagtac attttcatct cttcgacctg tgtaatacct ttgatcagct gcacaaatag
    22861 acaagtataa aggtcttacg atgacatgat taccataaat atcaacatta ttatatgtga
    22921 cgtcgaactg tctcggtgca acgagttgat atacttgatt aatcatcggc aatatcacct
    22981 tgaataatgg catttgctac ttttaaatca tacggtgttg tcactttaat gttgtatagt
    23041 tctccacgta ccaatttaac tgcatgtcca gattcgacaa tgattttaca tgcatctgat
    23101 aagatttctt tttgttcact acttaaggcg cgataactat cttgtaataa tttaatatta
    23161 aatgattgtg gtgtttggcc ttgatacatt tcattcctta cagggatact gtgtatgttc
    23221 tgtttatctt tagacattac aatcgtatca attgcttcaa tgactgtatc tactgcacca
    23281 tattttgctg ctacttcaat gttctcttta ataatacgtt gagttaaaaa tggtcttacg
    23341 gcatcatgag ttacaatcac atcatcatta ttaattccat ttacattgcg aatatggtcg
    23401 ataatgttca taattgtttc atttcgatcc gtaccacctg caactacttt gacacgttga
    23461 tctgtaatgt tatatttttt taaaatatcc tgtgtatggg aaatccactg tgctggcgtt
    23521 gcgataataa tctcattaaa ttcactcact aaaatgaact tctcaattgt atggattaaa
    23581 atcggtttat tatcaatatc taaaaattgt tttggtaatg gcacgttccc cattctcgaa
    23641 ccaatacctc ctgctaaaat acctgcataa atcatgttgt cctccattct gtcattacat
    23701 catttccatt tatacattac tgacctatgc ccgcacataa gcctaaccta ttgctcactt
    23761 gcctctttta ttaatccaaa gatagttgtc acaatagtgt gataattttt tataaaaatg
    23821 tatttttgta actgaccatt ctaagttgtt ttgccatgca gttaatcatt aactctgacg
    23881 atattaaatt gttaaaggta ttaatgttta ctctttttca aattcattat tactgccatc
    23941 attttaccat atattataat aaatttatct tattaagtgg ctgtacttga ttttcacttt
    24001 aaaaattatc aaatattgcc atctcatttt aagtatacaa aatgcaaaac aaccgattca
    24061 caagcatatt tcacacaagt aaaccggcta tttatcaacg tatattcgaa gatgaattat
    24121 ttcgatagta tctatagacc agacggcatt cgcactttca tagctataac tataccagcg
    24181 ttttcgtcct caaaggtgca tactaataaa tcgtaaacat gactttatca aatcgttctt
    24241 tcttgttaac taatttatca aatgtctccg ggcctttttc taacggtaaa aaatgagaaa
    24301 taataggctt tacattaata tctttcgtct tcatataatg taaggttgcc gtccactctt
    24361 tgcccggaaa attactggac aaacagttcc aagagccaca tactgtcaac tcgttacgca
    24421 gaattttttc aaaatgaacg cgatcaatct caatatcatc atatggtatt ccgagtaata
    24481 ccacctcgcc acctttttta ggtagcgtca atatttgacc aatcgtaact ttagcacctg
    24541 atgattctat agctaaatcg atttgattgg cgtaatgatt ttcgatgaat ttctcaagat
    24601 tttcttcttt tgaattgatt gtttgatgtg cgcccaatga tgttgcaata tctagtttat
    24661 gcgcatctat atctatagcg atgatatgtg cagcaccaaa tattcgtgcc cattgaatag
    24721 ctaacaaacc tatactgcca caccccatta ctgcaacagt cataccaggt tgtatattcg
    24781 atttataaaa cccatgcgca acaacggctg atggctcaac cattgctgct tcaatgtaat
    24841 caacattgtc tggaaccttt aaaacatttt gcgctggcaa tttgacatat tccgcgaacg
    24901 atccaggttc atatgagcca atgacgaata acttttcaca tcgtgcatat tcacctttta
    24961 aacaatactc gcattgataa caaggtattg ctgggcaacc tgtcactttg tcgcccacat
    25021 taacatgcgt aacatcactt ccaatggcat ctactacacc tgaaaattca tgaccaaatg
    25081 gcataccttt aatgtatggc cccatttttt ttgtatcgtg acgtgtctga accacatatg
    25141 ccagtcgctc gtactttaat aataacgtca ttcgcacttt caatgactgg cttttcatta
    25201 tcctcatacc gtaaatcttc cacgccatat aatttcaatg ctttcacttg taaatcacct
    25261 caaatttgat ttaattcaca acttttttct ttttaaaaat acctgtcgca aaataacctg
    25321 caatgacaat ggaattactt acgagtaaat gttccatata aaaatcagtg atttgtctta
    25381 atggcccaag cataaaagtt agcattgatt ctaccatatt aattctctcc ttcattttca
    25441 tctaaatcga gcggcttaag taatacttta ataccttcgc cggattttaa atgttgatac
    25501 gcctcgtccc atttcgaaat atcatatatt tttgtcacca aagcttcagc atttactaaa
    25561 ccatccgcca taagttgcaa tgaaggttcc caatctgctg gcttttgact tctactacca
    25621 acaactgtta tttctttttg aatcactttt tccatatcaa atggaatttc agcatcctta
    25681 aaaataccta tttgactgta gaaacctttt ttgcgtaaaa tatccaaacc ttgtcgtgct
    25741 gctggaactg cacctgaaca ttcaacaaca acatctgcac cgtaaccgtc tgtaattcca
    25801 ttgatatacg tttttaagtc tgtttgttgt aaattgacta cataatccat gtgcaatgct
    25861 tctgctttat ctaatctgac tttgtcattg tccaatccag ttaccacaac agttgcgcct
    25921 ttacttttta acacttgtgc tacaagtaat ccgattggcc caggtcccat tacaactgct
    25981 acatcgcctg aattgacttg aatcttagaa acgccatgat atgcacatgc taatggttct
    26041 gtcatagctg cagactgata cgatacttcg tctggaatat gatgcaaact ttcttcacgt
    26101 gcaatgacat aattagtaaa tgcgccatca acttgtgttc caataccttt tcgatggttg
    26161 cataaattat agtcttttga tttacagtat tcacactcat tacaaacata gaatgtcgtt
    26221 tcagatgtga cacggtcacc aactttaaaa tctttaacgt ctgctccaac ttcaacgatt
    26281 tcaccagaaa attcatgacc taatgtcact ggaaaattaa ctttataatg accttcataa
    26341 gtatgaatat ctgtgccaca aattcctgca taatgtactt taatctttac tttatcatct
    26401 agcggtgttg caacttcttt atcaagaagt tctaagttgc catgtccttc tcttgttttt
    26461 actaaagctt tcaccacaaa cacctcgatt tttaattgaa tagactaaat agtttaaaga
    26521 taagatagtt aacgatatta ccaccttgat caatacttga aatttcagat gaaccttttg
    26581 gcatttgtac attcgtacct ttcgccatat ctgtgaaaat gggtgctacg tctgttgcaa
    26641 tatatagtga aattgcaatc ataatcgtac ccacaatgac agaatgaata atgtttcctc
    26701 ttgctgcacc aacaataaac gcgacaacaa atggtatcgt tgctaagtca ccaaaaggta
    26761 gtacttggtt tcctggtaaa ataacggcta ataaaacagt gataggtact aaaattaatg
    26821 ctgtcgaaat aactgctgga tgacctaatg ctacagccgc atccaatcca atataaattt
    26881 cacgttcgcc aaaacgttta tttagccatg ttcttgcaga ctctgaaact ggcattaaac
    26941 cttccattaa gatttttacc attctaggca ttaataccat tactgcagcc attgacattc
    27001 ctaaattaat gatgtctcca ggtttgtaac ctgctaacac accaatacct aaacctaaaa
    27061 tcaagccgac aaatatagac tctccaaatg cgccaaaacg tttttgaatt gtttcaggat
    27121 cagcatctaa cttattcaga ccgggtactt tttgtaacaa tttaactaag taaatacctg
    27181 gtgcataaga aattgtactt cctgtagcaa tcgatacacc tggtaaatca aagaactcac
    27241 tcatcatcgg tgctgtccaa tctgctactt tcaaacagat aatttggaaa ataactgctg
    27301 ctaataacgc ttgccaaata ctgcctgata cggcataaac cattgctgct gtaaacgtat
    27361 aatgccaaaa attccaaata tctacattca tcgtctttgt cactttagtt actagcaata
    27421 caacgttaac tatgattccg agtggaataa taaatgctgc gacagatgat gcccaagcga
    27481 tagatgatgt tgctggccaa cctacatcaa tcacattcag actgacgcct aaatttttaa
    27541 ccatcgcttg tgctgctggc cctaaatttt taactaataa atcgatgact aagaaaatcc
    27601 ctacaaaagc cacacctatt gttaaaccag acctaaatgc cgctccaatt ttctgcctaa
    27661 agaataggcc aagcaagaat atgacaaccg gtaaaataac agttgcacct aaatctaaaa
    27721 atccccttac aaaatcagtg aagtaactca tatttaaacc ctccctgtta tatatgcatt
    27781 gtcacgatac tttccgattg tgattacatt tgacgttaca gtcatttcaa cgacaaccct
    27841 tgctaaattc gactgcagtc cttttgaatt acagtcactg cgtttctatg tcatcaacaa
    27901 tcatttgtcg tgatagtcat ttatatgcaa tttgcatata ttaatatgtt atcgacccac
    27961 gttacatatc aattccgtta tttttgtaac tctgttaaga tttgttgttt tgtttcttca
    28021 ataccaatac cagttaagaa attacgtgcg ttgataactg ggaatttata ttcttttttt
    28081 gtcattgcag ttgtaactaa taaatctgca gtgtcttcat aaggtccaac ttctgtaatt
    28141 ttgatttgtt taatatctac tttaatattg tgttcctttg ccatttcttc aattgcatta
    28201 tttactactg ttgacgttgc aatacctgca ccacacgcta ctaatacttg tttcattttc
    28261 aattcctcca attaattttt agttatattc caaataatca ttgattagtg ttgctaaaat
    28321 tgtttcatct ttcgttcgta gaatctgctc caatttttct tcactttgaa aaatttgcat
    28381 caactgttgt aacagcttaa gttgatcatc tactttatcc attgctaaca taaaaacgat
    28441 tttcacttct gtctgttgat caagtgttcc catttcaata aacggcactt ctttttctag
    28501 aacagccaca cctatcgttc tatggttaat atgttcgaca tctgtatgcg gtatagcgac
    28561 cgaacataga tgcgttggta aaccagtagc aaattctttt tctctgtcga tgactgcatc
    28621 tttaaacgtt gacttcacga acccattttg aaataacaca tctgacattt gtgacaatac
    28681 ggattcttta tcagttgccg acaaattgag cattatattt tctttatgca ctaattgctg
    28741 tcccatccat tttccctcgc ttctttattt gaataatttt ttaaaatctc atttacatca
    28801 gaatttttgc gactttgtat gatgcgctta attgcgtcat tgtcttgcgc cacatctctc
    28861 aattgtagta acgctcttaa gtgtgtcact ttatcaacag cagcaatagg tacaataata
    28921 tggattgctg tgccatctga catgtatatt ggttcttgta atatcaacat actcatcgct
    28981 gttttatgta catgcttttc agagtatgca tgtggaatag caatattttg catgataacc
    29041 atatacgaat catcaaatac ctgttgcatc tcatgaatat atttaatatc aataaaatga
    29101 ttagcaacta acacatcact tgctttagca atagcttcat caatattttc aacatgatgc
    29161 attcttttca cgtgccttgc cggtatcaag tcagctaaat ctaatgtctt attttgtgtg
    29221 acaatcgatc cattaatggt tgaaattgaa ttataattgg caataaaatc ttctaaacca
    29281 tcacgtagtt ctgtaatgtc attaactgtc gttgtgcgtt caattaatgc cattaacttg
    29341 tttatttcct tatcaatgtc agccgattcc ttattaatgt acttcatcac ttctttacgt
    29401 aactttcgtt gctcattttc agataaagct acttttgtga taaataattt tttatgtgtt
    29461 aggacaaaca ttggtgaaaa gacgatgtca taatctaatg tgtaattttc aaatgttcta
    29521 agtgaaatcg catctaagaa aataatttct ggaaataagt ttcgcaactc gtataacatc
    29581 atttgtgata ctgacgtgcc ttgtgtacac acgataatag cttttatctt gccatcgaag
    29641 ttttcatctt gacgtctcaa actacctccg aacaacatgg ttaaatatgc tatttcatta
    29701 tcaggcaacg attttccgaa atattcagtt aacgattgac atgattgttt caccatatga
    29761 aataaggatt gataatttcc ttgtaaagga tttattaatt catcacgatc cgttaagtta
    29821 tatttaatcc tataaaaagc aggcgttaaa tgtaacaaga gttgctgtga taatttctcc
    29881 ttatcttcaa tgttaataaa agtgatttgt tcaaaatggt gaatcatttg agcgatggcc
    29941 atcgttaaat tcgatatgct atctgattct tgcaaatcag tccattgcac acttgttgaa
    30001 agtaagtgta atgtcaaata taacttttcc gcttctggca aatccggctc atgttgcgtc
    30061 ataatctccg ttgcttgata ttctttcgta tccctcaaat actgataatt aatatttaat
    30121 ggattcatca catgaccact ttgaattcgt ctacgaatca cacaaaggac ataaggcaat
    30181 gaactaagtg atttgtctat aaagcgactc ttcaaaaatt gttctacctg tttgatcttg
    30241 tctttttgat atgcgatatc ttcgaatgtt aagttgagcg cctttaaaac ttcactttta
    30301 gtaatatcat gattcaacct ttgatcaatc aacttaatga agaaacggcg aacttcaaat
    30361 tcatcaccaa caatttcata accatgtttt cgagaatact taagtgacaa accatgattt
    30421 tccaattgct ctttcacatg atttatatcg tgaatgacag tatttttact gacttgtaaa
    30481 tcaattgaaa aatggtttag agacattgcg ttttccttac taaaaagcat gagcattaaa
    30541 taataacgac gtgtttctat gctaaaaatg acattgttgc cgtttaacat ttgctgctcc
    30601 gatacatctc gcttgaataa cgtcatgatt tcagaactta caataaaatt tccttggctt
    30661 gttctttcaa gttttggata accctcttgt tcaagccaca aattgatttt ttgaatgcga
    30721 tatcctagtt gtctacgaga caaaccaaat atcgattcaa gttctttacc atgaatagta
    30781 ggattcaata caatttctct gagtatttgc tgattatccc cgttcatgtt cgattctcct
    30841 ttgagcatct gagctcaatt tatcatcaat cgtcacaccg atgtacacac tttgaacaca
    30901 tattttcaaa atgagcatgt acatcattgt gatgttttaa caacatttca attatatcta
    30961 tatttttgtg attttaatct tttaaaataa agcaattgaa atttttgcat atatttttgt
    31021 gttttgtgtt tttttgaagc atttttaaca tacatatctc aatcattatc aaattgtcat
    31081 gaccattgta acccaataca aaaaccctaa ggacgcttat atcaggcgcc ttagggttaa
    31141 ctgtatctat ttaattaagt attattattc gtatgtacgt aacttatggt ctatcaagtt
    31201 ccacacttct tcaacatcaa ctgctgtagc aaaataagca ttggcaggct tacctgtaac
    31261 atgatttaaa tcgacagcca tagtgccata agttagtgga ctttgatgtt caatgtcgat
    31321 attaacgggt accattgtaa acaattctgg ttgtaacaaa tacaaaattg tacaagcatc
    31381 atgtattgga ccaccatcca tattaaagtg agtcttgtat gtcttcttaa agaattgcaa
    31441 taattctacg acgaactgtg caacaggatt attgatactt tcaaagcgtt caatcacgtg
    31501 atcgtcggct aaaacttgat gtgttacatc taaaccaaac acatttatag taatcccact
    31561 ttcaaaaaca cgcttcgctg cttcagcatc tacccaaata ttgaattctg ctgtaggcgt
    31621 ccaatttcca aatgtaccac cacccatcaa agtaatagat tcaatatgct cagcgattct
    31681 tggctcacga atcaatgccg ttgctacatt cgtaagagga cctgtcgcta caattgttac
    31741 aggtgtatca ctcgtcatca ctttgtttat aatcacatct gatgctggca ttgcaactgc
    31801 ttgacgtgat ggtgtcgacg gtagtttcgg accatctaat ccagattccc catgtatttc
    31861 agaagcaaag gcagctggtt taattaacgg cctatccgca cctttcgcta ctgctatatc
    31921 ttggcgtccc ataatatcca atacgttcaa ggcgtttgtc gtattcttgt caactgattg
    31981 attacctgcg actgttgtta cagctaatat ctctagtgga ctgtcaattg cccccgctaa
    32041 aattaatgct attgcatcat cgtgtcctgg atcacaatcc ataataatct ttcttttcat
    32101 ttatatatcc acctttctta agttgttatc gatagcttat gtatatttat ttatgtggtg
    32161 aatcatgttt attttgaaaa atagttttaa ctttctcata tttttggata caaacactat
    32221 ttatctattt tatggcttat aaatttatcc gatatgcctt atcaacctac ctcgctaaaa
    32281 ataggatgtc tacatatcta taccgacttt tgtcaactca ttttcacaac aatataaaca
    32341 gcaatttata tgattgttac atgattcaaa caatttttat gaaaaatatt ttcatacaca
    32401 gaatatatat tgatattaaa tttctcaaaa gctatattga gaataattag gagggatgtt
    32461 gatgaaatct ttatttgaaa aagcacagca gttcggcaag tcctttatgt tacctatcgc
    32521 aatcttacca gctgcaggtc tattgttggg tatcggtggt gcattaagta atccaaacac
    32581 cgttaaagca taccctattt tagatattac cttattacaa aatattttta cattaatgtc
    32641 agctgcaggt agtattgttt tccaaaattt accggtcatc tttgcaattg gtgtcgcaat
    32701 cggattatct agaagcgata aaggtactgc aggtttagct gcgctgctcg gtttcttaat
    32761 tatgaacgca actatgaatg gcttattaac tatcacgggc acattggcaa aagatcagct
    32821 tgcacaaaat ggacaaggca tggtgctcgg tatacaaacg gttgaaaccg gtgtttttgg
    32881 cgggattatc acaggtatta tgaccgcaat acttcacaac aaatatcaca aagtggtatt
    32941 accaccgtat ttaggtttct ttggtggctc tagatttgtc cctattgtca cagcatttgc
    33001 cgcaatcttt ttaggtgtat tgatgttttt catttggcca agcatacaag ccggcattta
    33061 tcatgttggt ggatttgtaa cgaaaacagg tgccatcggt acttttgttt atggcttcat
    33121 cttaagattg ttaggtccac tcggtttaca ccatattttt tacttaccgt tttggcagac
    33181 ggcacttggt ggtactttag aagtcaaagg gcacttagtt caaggtacgc agaacatctt
    33241 ctttgctcaa cttggtgatc cagatgtgac gaagtattat tcaggtgtgt cacgctttat
    33301 gtcaggccgt tttattacga tgatgttcgg cttatgtggt gccgcacttg caatttatca
    33361 cacagctaaa cctgaacata aaaaagttgt cggcggttta atgttatccg ctgcactcac
    33421 ttcattttta acaggtatta ccgaaccttt agagtttagt ttcttgtttg tcgcacctat
    33481 tctttatgta atccatgcct tctttgatgg attagcattt atgatggcag acattttcaa
    33541 cattacaatt ggtcaaacct tcagtggagg ctttatcgat ttcttactct ttggtgtgct
    33601 acaaggtaat agtaaaacaa actacctata cgtcatacct attggaattg tgtggttctg
    33661 tttgtattac atcgttttca gattcttaat tacgaaattt aatttcaaaa cacctggtcg
    33721 agaagataaa gctgcagcac aacaagttga ggctactgaa agagcacaaa ctattgttgc
    33781 tggtttggga ggcaaagata acattgaaat cgttgactgt tgtgcaacga gactacgcgt
    33841 cacacttcat caaaatgaca aagtcgataa agtattactc gaaagtactg gtgccaaagg
    33901 tgtaatccag caaggcactg gtgtgcaagt aatttatggg cctcacgtta cagttatcaa
    33961 aaatgaaatt gaagaattgc tcggggatta agactaaccg aaatatcaac agaactaatg
    34021 gcaacgatgt acgaagtaag aagtgacatc gttgctttta tttttaatgt tacatttgaa
    34081 gcattaagtt catcatgcac tgtagtgagc ccgcaaatcg cctctgctag acaatcatct
    34141 taatgctatg attaaagctt aagtgccaga tttgaattta atttcaacaa cgactttcac
    34201 tacattaaaa atagggctac tcgacacata tagttgtatc aaatagccct ttatacaatt
    34261 ttttgggtaa ggttttacaa tttttgggat ggtatagatt ttataaaaag ttatttaagt
    34321 tcttctgctt cagccataat atcttttaat gttttagctg aatgtgcgaa cttgctttgt
    34381 tcttcgtcgt ttaatgggat ttctacgacg ttgcgaatac cgtttctatt gatgactgct
    34441 ggaacaccaa tataaacatc ttcttcctca tattcgcctt ctaataatgc tgatacagtc
    34501 aatacggcat cttcatttct gaaaatcgct tcagtaattc tagctaatcc cattgcaaca
    34561 ccataataag tggcaccttt agcttgaata atgtcatatg ctgcatcacg tgtttgaaca
    34621 aaaatttgtt caatttgcgc tttgccctca ggacgttgtt caagtaatgt cttcaaaggt
    34681 tgacccgcaa tattagcgtg tgaccatact ggtaattcag tgtcaccatg ttcaccaata
    34741 atttgagcat cgacgctacg tggcgcaaca tcgaacgctt cgcttaacaa taatctaaag
    34801 cgtgcagagt ctaaaattgt accagaacct ataacacgtt ctttaggtaa accagagaat
    34861 ttccatgttg catacgctaa aatatcaaca ggatttgtag ctaccaagaa aataccatca
    34921 aattttgatg ccattacttc accaacaatt gatttgaata ttttcaagtt tttagatact
    34981 aaatctaaac gtgtttctcc aggtttttgt gcagcaccag cacagatgac aactagatcc
    35041 gcatcatgac aatcactgta ttcgccagct ttcacacgaa ctgttgttgg agaatatggt
    35101 gtggcatgtt ttaaatccat aacatctcct cgaacttttt cagtgtctaa atcaatgatg
    35161 actaattcat caacaatgct ttggttcact aatgaaaatg cgtagcttga acctactgca
    35221 ccattaccta ttaatacaac tttgttccct ttaaatttgt tcattacaaa aactccctta
    35281 tgattaattc actaacatac atgtagcttc aaatatgtta gtttaatgct gcttattgac
    35341 gatacaaaag caaataaaca tctcttttat tttcaacgca taacttaaaa ggtcatgtgt
    35401 catccgcttt taagtttgtg atttatttca catataaaat gtaacatgca ttaagtactg
    35461 ggtcaatatt aaattgtgat ttatttcaca ttttatttta atttttacac ctttttaatt
    35521 tgtatacgat tacatcttag atgtctttag tcttcgtact tcgccagtga ttatttacac
    35581 tttcacattt ttattatcat gtttactttt ttctaggaaa acaacaatgt tttttgaatt
    35641 agtcaaataa atgcgctcaa tcgtcggtgt gcaaacagac aattgtacac aatgcttatt
    35701 gataagtatt taaaaaatta aaaatgtcat acaattatca aatttgccat tttatttata
    35761 ttttctcaaa ccaattaatt gaatatcgaa atttttagta gaataatcaa aatatacaga
    35821 ttaaaggagg agtatcatgc ttacagaaca agagaaagac attatcaaac aaacggtgcc
    35881 tttacttaaa gagaaaggga cagaaattac gtcaatcttt tatccaaaaa tgtttaaagc
    35941 gcatcctgaa cttttaaaca tgtttaatca aacgaaccaa aaacgaggca tgcaatcttc
    36001 agcattagca caagctgtaa tggccgcagc ggttaatatc gataacttaa gtgttattaa
    36061 accagtcatt atgccagtcg catataaaca ctgcgcacta caagtttatg ctgaacatta
    36121 tccaattgtg gggaaaaatt tattaaaagc cattcaagac gtgacaggat tagaagaaaa
    36181 tgaccctgtc attcaagctt gggcaaaagc atatggcgtt attgcggatg tgttcatcca
    36241 aattgaaaaa gaaatttatg atcaaatgat gtggattggt tttaaaccat tcaaaattac
    36301 caatattaaa caagaatctg aagacattaa atcatttaca gttgaaactg aagaatatga
    36361 ctttagtgaa tttacaccag gccaatacat cacagttgat gtttctagtg ataaacttcc
    36421 atatagagct aaacgtcact attctatcgt atcaggtgaa aaaaaccatt taacttttgg
    36481 cgttaaacgt gatgtcacaa cagaacatga aggcgaagtt tcaacaattt tacacgatga
    36541 gattaaagaa ggcgatatga ttaatttagc tgcgcctgtg ggtggattcg tattagagaa
    36601 tacgactgaa ccacaacttt tcttaggttc aggtattggt gttacacctt tagtagctat
    36661 gtatgaagct gcctctgcca aaggtttaga tacacagatg gttcaagttg ctgaaaatga
    36721 acaacattta cctttcaaag acaacttcaa cagtatcgca agccatcatg acaacgctaa
    36781 attatataca cacttaaaag ataaacaagg ctatattggt gctgaagaat tacaagtatt
    36841 tttagcaaat aaacctgaaa tttatatctg tggtggtaca aaattcttac aatctatgat
    36901 cgaagcactt aaatctttaa attacgatat ggatcgcgta cactacgaaa catttattcc
    36961 aagactaagc gttgcagtat aactgagaaa agaggtttag tgctcatatg aaacttaatc
    37021 aacgttacgt aaaagtattt gcattatatt tcgtaagtat tgttactgca aatattattg
    37081 ttaaaaataa taatttaatt aaaacattga tacaaaccat agccgggtac acggtctttg
    37141 cagttggttt gaagtattta actaaacgta aaaataaatg acatctatac caaaacagct
    37201 atgactttac tttgtcatgg ctgtttttta attgggagta ggacagagat gatattttcg
    37261 caaaatttat ttggtcgtcc caccacaaca tgcattgatg tatgctcact gaatttcata
    37321 agaaaggagt tcacaagatg accgtagata ttggacggat ttatgacaat aaagataata
    37381 ccgacgctat tcgtatccta gtcgatagag tctggccgag aggtatttcg aaaagaactg
    37441 ctaacctaga ttattggtta aaagacattg ccccttctac tgagttgcga caatggttcc
    37501 aacatgatcc taaacttttt ggagctttta aagaaaaata tgaaaaagaa ttacgtgatc
    37561 aggatgcgca aaaagatgct tttgaaaaat taaaggatat tgtaaatcag cataatcatg
    37621 ttctattgtt atatgcagca aaagatacta aacataacca agctgtagta ctacagcagt
    37681 tgctcaatac ttagttatat atgtttatcg tgaatcacta taaatgtcgc taacttcatt
    37741 tctaatattt atttttaaaa agcataatta ctacaattaa ttgaacttta ataattacta
    37801 aacttgaaca acattttact ttaacaaaat aaagttttaa aatattaatt gttgggtttt
    37861 acatagtaat tagaaagaga gtgttaatga aatgaagttt aaaagactag caactatatt
    37921 ttcagcagta ttagtgttat caggatgtgg ctcaatgcat tcatctggca aagacttaaa
    37981 tatttcgtta ccgttaaaaa caaagtctat tgcaccttat gaaacagatg taccagttaa
    38041 gattggtgct gcagaatcat tatttaaaac aaatgatcaa ggaaaaatag aaaaagcatt
    38101 agtgaaatcg tatcatcaac caaatgatac aacgttagat atagaattaa aagataatat
    38161 taaatttcaa aacggtcaaa aattgactgc agaaaaagtg aaatctagcc ttgaaaatag
    38221 catgaaaaaa agcgacttgg tcaaatattc attaccaata tcatcaatta ccgctaaagg
    38281 tcaaaaactg acaattaaaa ccaactccgc ttaccctgaa cttgtatctg aattagctaa
    38341 tccttttatg gcaatttatg atacagatgc taaatcagat gttaatcaaa ctcctgttgg
    38401 tacaggccct taccaaataa aagattataa gcaatctcga aaaatatcat tgtcgaattt
    38461 taaggactat tggcaaggta aaccgaaact tgatcatatt actgtgacgt accaagaaga
    38521 cggcaataat cgcgtcagaa atttagaatc tcaaaaagat gatttaataa ctgatgtccc
    38581 agttaataaa gttcaagaca tagaaaataa tcaaaattta aaagtgtcaa aagaatctgg
    38641 atttagaact tctttactta tgtataatca tactaataaa aaaatgacta aatccgttcg
    38701 tgaagcatta gatcatatca ttgatagaca aggtattgca gatcatattt atcaaggtta
    38761 tgcgaaacct gcaacgagtc catttaatga taaaattcca tatattaaag aacctaagtt
    38821 aaccaaacaa aatatcgaac aagcaaaaat gttattagct aaagatggtt atacaaaaga
    38881 acacccttta aaaattaagt taattacgta tgacggtcgt ccagagctat cgaaaattgc
    38941 gcaagtatta caatcagatg ctaaaaaagc aaatattgaa atcgacatta aaagtgttga
    39001 tgacatagaa ggttacttaa aagaccgttc tgcatgggat gcaacgatgt atagtttcgg
    39061 aacaattcct cgtggagata caggttattt ctttaatcaa gcatataaaa aagatggtgc
    39121 tattaataaa ggagactaca ataatagcaa cgtcgatgat ttaattaatc aattgaatca
    39181 tactgtagat gttaaggaac gtcacaatat ttctaatgat attattaaat tatcaagtcg
    39241 agatgtacca aacagttata ttgcttacaa cgatcaaata gtcgctgcta actctaaggt
    39301 taaaaattat aaagtaacac ctgaaggtat ctatttaatc gattatcgaa caacgattga
    39361 acgataaggg agtgtgggac attgatttat gtctcagccc tgcttcaact ataaaaacca
    39421 cgccgaagtc atccaatatg attatggcgt ggtttaattt gcttatatga ttgtattaat
    39481 ttctgtttga tgaatttcaa taaaatgatt attacatact ttcagtgaat catgttcatt
    39541 ttatataaat ctaattgtca ctatttgttt aacgtgaaac acttttttaa ctccaaaaag
    39601 tattcctatt ccactctttt aaagacaaat tataaaaata gcttatgtca caaacatatg
    39661 agcaacataa gctttaatta ttattcgtct acttttttct gttgttcttt aaattctttc
    39721 tgcagtaaat tcacttgttt taaacctgcc cccattaaaa tgaatacaag tatccacaca
    39781 ataacgatta aaataaatat tttcaacgta tcactgccaa gtacagttaa agatgtatcc
    39841 caaatgccat gtaaaacaac ggctgataaa aagaatatta aaaagcgttt atcaaaaata
    39901 tctttgaatt caaagccatg ctgttctttc gcaataacta ttgcagcacc aacaatcgct
    39961 gaccaaacta aatgaccacc aatcgcagtc cacgcacgtg tgaagacaat atctaataat
    40021 gggacatttt ctcctaaagc gaaattcaaa atataacctg ctgattcaaa aactgcgaac
    40081 cctgcaccaa tagcagcacc gattaataat ccattcaaaa tcttatttgt tttcaattta
    40141 ttgacgaaat aaacaataat gagtgctttt ccagtttctt caactaatcc tactaaaaat
    40201 gcatcgaaaa atgttaaaga accaaacctt tcgaattgat cactaaaaac gacaaatcta
    40261 tataatacca tcgtacttag taatgaaaat acgccgccaa taaagaacat gataataact
    40321 tcaaaaatgc taatattttt aaacgcattt gattcataaa agaagaacaa acccgataat
    40381 ggtactgtta aagcccctat aaaaatgaga cccggaatcg cgttagtgtt attaaaaatt
    40441 gctgccatga cccataatcc aataaatgtt actgtgaaag cgatgaatac tcgagaaaag
    40501 agccatggct tcccccattc ttctgatatg tcacaaattg ctggcgtagt tttagcagta
    40561 cccgctataa atatttcatc tgcttcgttt ttagtatgcg gcttaaatac ttcagaaagc
    40621 atatctttta aattaagatt taagctttca tcattgcccg ctagtctacc gattgatttg
    40681 gttgcattat caaataaact tcttgcttcg ttttttaaat ctgtattttg cgctgtttca
    40741 tgcgctttag cttccatttg tttatcgata gattcattat gtatgtcgga cgtcttagtt
    40801 gattgtgtac ttgctgatga catcccctct tcacctgtat taggtgcaac tgattgcgtt
    40861 tgtgttgtct cttctttttc tgtatttctt cgatgattaa gaccttccat agacttacct
    40921 actttcactt ttcataataa aactatttta atttaatatt gatggatttt ccatatttta
    40981 atataaatac tttgttcgat tgggcacttt gtatttcata tttaaaaatc aaaacctatt
    41041 taacttttta tattccgaac aatattttta tataagtatt ttaatgatat tcaattcatt
    41101 acaaaccctt gatattacaa ttgttatcga ccgtcaaaat gtgtctatac tgtgtctatt
    41161 atcagaataa aaagatattt ctaattgatt ttaaacgtcg ttatgttata tttcttgtaa
    41221 gggtttacac aaagtgtaaa aacgctatat tttatcaaat gtgatggagg tgacttgctt
    41281 gaaacaaatc acatggcacg acttacaaca tatcattaaa gatggtgatg tgattggttt
    41341 accagcatta gctgtagcca acttacccgc cgaagttcta cgtgctgtgt tagcgcaaca
    41401 tgacacatat catacgccca atgatttaac gtttatatta gcgaatgata ttcatagttt
    41461 aggtgccgca ccggatttag atgattttat agaacgtcgc atgattaaac gtgtcattat
    41521 gagtatttta acggcttctt ccaaaacggc acaagcaatg aaaaataatg acattgaagc
    41581 ttatttttta ccacaaggta tcattgcaac tcattatcgt cagagtaatc aattattacc
    41641 tggagttatt actaaaatcg gattaaacac agctgttgat cctagatacg gtggcggtaa
    41701 agtaaataca cgaacaacgg atgatttagt ttcattagta accatcaacg atgaaacata
    41761 cttacattac acattcccta gcgttgatgt ggcactactg agaggaacat acgcagatca
    41821 acaaggtaac atttatttaa ctcaagaagc gtacttgagc gagtgttatc atgtcgcatt
    41881 aaacgcgaaa gccaatcatg ggaaagttat tgtacaagtt aaagctttag ttgacgacta
    41941 tcaactaaaa ccgaatgaag ttgttatccc aggaaatctt gtcgattatg tatacgtcac
    42001 agaagatgaa aagaatcacc gccaagtaat tcaaagtcat tatttaccag ccttgtctgg
    42061 agaagaacga attgatggaa tacctgaacc cgcattacct tttaatagtc gcaaattgat
    42121 tctccgacgt gctgctcagt ttttaactta tggcgataca attagcatcg gttatggcat
    42181 caataatgaa ctctctaatt tattgcatga agaatgtgtt gaacatgatg tgcaaccgat
    42241 tttagatgtt ggcattttcg gtggattcgt tgggagtcgt gaacattttg gtatgaatta
    42301 caatgcagat gtgcgcatgc ctcatgatcg agcatgggat tttatttata acaatggtgt
    42361 atcagttgcc tatcttagct ttgctgaggt tgatcaatac ggcaatgtca acgtgtctta
    42421 cttcaatgac cgactaaatg gatgtggtgg ctttatagac attacgcaat ctgtaaataa
    42481 aattatcttt tcaggtactt ttgtagctgg cagtcatgtc tcatgccata atcaacgatt
    42541 aaacattgaa actgaaggac aaaaccagaa atttgtatca gatgtgagcc atatcgactt
    42601 taatgcacaa tattcacaat cactcgagca agaaatctat tttgttactg atcgtgcagt
    42661 attcgaactc accaatcaag gcttgaaact aattgaaatt gcaccaggtc ttgatttgca
    42721 taaagatatt ttgaatcaaa tggcttttaa accaattatt gctgatcatt taaaattaat
    42781 tgataccagc atttacaaag aaaaatgggg acaacttaaa caatcaattc ataaagtatg
    42841 aaagtgaagc taaagggggt atactaatga atttcgattg gataaaaacg cgttcagact
    42901 tcgatgatga caagcctgcc gttattgatc acgcaaaaca aacatcttgg acataccaac
    42961 aactcaatgc acgcgctgat aatatggcac attatttaac atctcaaggt gttaaaaaag
    43021 gcgatgttat cggtattttt gcgccaaatg atattgcaat attagattta ttgtttgctt
    43081 gttttaaaac aggtgcggtt tttttaccat tgaattggcg gcttaaccca aaagaaattg
    43141 cagccattgt ggaagatgca caattaaaac tgctcttcta tgctgaaaaa catttaagtt
    43201 cactcaccga tattgaccaa aacttattgc atatggatat tgatgtggcg caatatgatg
    43261 aaatcgtaaa tccagattat caccaacctt ttcaagcaac acctgttgaa ccacaagatc
    43321 ttgcagcatt aatttataca agtggtacaa ccggatcacc taaaggtgtg atgttctcat
    43381 atgaatcatt tgttcataac ggtgcaaact tagagctgac gtataagttc aattcaaact
    43441 atattacgat tgtatcaaca ccaatgttcc atgttttagg gtttaacgat actgtattac
    43501 cagtattaat gtcaggtggc acacttattc ttcaacgtta ctttaacggc gaagaattga
    43561 atgacatgat tgcacaatat caccctacat ttattatcat gattccgaca atgtattata
    43621 gtacgctacg tgctagtaat tttaatcctg aaaattttag agctatggat tatatcatcc
    43681 aaggtggttc acaaccatta ccaagtatcc aagcagcttt taaacaatat ggcataaaca
    43741 ttattaacgg ctatggttta actgaagcac ctcttgtact tgttaataca ccggaaaatt
    43801 caaaacgtaa gccaatgagt attggtaaag cagtcatgtt cgttgatgca cgtatccttg
    43861 atgataacgg tgaggaagta cctactggtg agattggcga acttgcaatt aaggctaaaa
    43921 atgtcacgcc aggatattgg aataaaccag cagagactgc caaagcattt catggtcgat
    43981 atttattaac tggtgactta gcgaagatgg acaacgatgg cgatatattt attattgacc
    44041 gcaaaaaaga attaatcata actggtggcg aaaatgtctt accatccgaa gtcgaaaatg
    44101 ctttagctga gcatccacta gtagaccggt gtgtggtcgt tggctatgat catccaaaat
    44161 atggtgaatc aattgctgca gccattatac ttcgcgaaga tgaacctcat tacgctgaaa
    44221 ttttaaatca acatatgcga agtcgtttag caggttataa agtcccaaga atgtatgtac
    44281 cagtgacaca tatgccgtta aacagtacgc agaaaccaga taaacttgcg attcgacaaa
    44341 tgatgaatga caaagtctcg caaacacttt aaaggtgata aaaatttttg acatttagtg
    44401 taagcgttta caaataaagc gtgttgtttt tgaattaaat gcatttcaca ttagtattca
    44461 tattattttt aggaggaatt tatatgacat ttgaaaaaga aacggtctta aaaacattat
    44521 ttcctgaaga tgtacttagt attgctaaag gtttaacaga cggtgaagtc gaatttttac
    44581 aacaagtaga ttcattgcta gaaagtaagt accgtgaaaa tattaatcaa cattggatag
    44641 acgctactgt acccgaggac tattttaaag atctgggaga attaaattat tttaacaatc
    44701 cattacttta caaggatcgt ccaaacgcca aaatgcctag tcaactattt cagtttttca
    44761 tgtcttacct actcgcgcga tttgatattt ccttagctac cctactcggt gttcaccaag
    44821 gtttagggca taacactttc tatttcggag gtagcaaaga acaaattgcg aaatatgtac
    44881 ctaaattaca gtcacatgaa ctgcgtacat gctttgcttt aactgaacca gaacacggtt
    44941 cggacgttgc gggaggtctt gaaacagtcg ctgaacgcca aggcgatact tgggttatca
    45001 atggtgaaaa gaaatggatt ggtggtgcac atgtatctga tgtcattcca gtattcgcag
    45061 taaataaaga aactggcaaa ccccattgct ttgtagtcag accagaacaa gatggcgtcg
    45121 atattgaagt cattgataat aaaatcgcac ttcgcattgt tcctaacgcc ctaattaaat
    45181 taactaatgt caaagtagat gaagcggatc gcttacaaaa cataacaagc tttaaagata
    45241 ttgccaaaat tctttattca acgagagcag gcgttgctta tatggctaca ggtggtatgg
    45301 ctggcgcttt acgtgccaca ttagattatg tcactgagcg taagcaattc ggcaaaccaa
    45361 ttagtaaata tcagttaata caagaaaagc tagcaatgat gcaaggtaat ttagctcaag
    45421 caatggcaac atgtgctcaa ttagctaata tgcaagcaca tggtgaatat gacgaggttg
    45481 caacttcaac ggcgaagatg atgaatgcct tacgtttgcg tgagacagta gctatgggcc
    45541 gcggtattac aggtggtaat ggcatactag ctgacgatta tgatattgca cgtttcttct
    45601 ctgatgcaga agcgatttac acgtacgaag gtacacatga aattaatgcc ttagtaattg
    45661 gacgcgcttt gactggagat tctgctttcg tataaatagc aaataattat atgagatgca
    45721 ttaatttcac taaaaaagac ttattttaag cataaagctt tttccttaaa taagaggcta
    45781 agatgactgt caaagatact taattaattt tataaaatag caacgttatt ccaattatct
    45841 taatggttat cttatcctca actaaattgg aggaatcact atgacaatta ataaagtaac
    45901 cgttcttggc gcaggcacaa tgggcgctca actggcagca ctttttgtga atgctggact
    45961 taaagtaaaa ctattagata ttgtagtgga caaaaacgat ccaaatctca ttgcgaaaaa
    46021 atcttacgat aaaattacag ataagaaacg gccgctacta ttcgacttaa atctagcgag
    46081 tcatttaaca tatggtaatt ttgatgatga cttggtaaat gatgatgctg atttatatat
    46141 cgaagcagtc aaagaagata ttgaaattaa gcatgctgtt tggcaacaag ttctacaaca
    46201 tgctaaagaa gatgctttat tcgctacaaa tacatcaggt attccaatta atgcgattgc
    46261 tcaagcattt aacgagaagg atcaagaacg attctttggt ctacatttct ttaacccacc
    46321 acgtattatg aaattagtgg agttaatacc tacgtcacac acgaaggaat ctattatatt
    46381 agatgtaaaa aatttcgcgc aaaatgtgtt aggtaaaggt gtcattgtcg tcaatgatgt
    46441 gcctggcttt gtcgcaaata gagtcggcac gcaaacaatg aatgatatta tgtatcgcgc
    46501 cgagcaacac aagataagca ttgtagatgt ggatgcttta actgggcaag cgattggtcg
    46561 tcctaaaaca ggtacatatg cgctatctga cctagtcggt ttagatattg cagtgtctgt
    46621 aattaaaggc atgcaacaag tacctgaaga aacaccttat tttcatgatg tcaaaattgt
    46681 aaatacgttg tttgacaatg gcgcactcgg acgtaaaacg aaacaaggat tttacaaaaa
    46741 ggataaagaa actaaagctc gacttgttta cgatgttgaa aaacaagatt atgtacctgt
    46801 atcgcaacca caattaccaa ttttaaatga atttaataaa gacttagtgc ataaccttga
    46861 taccatattc aatgcgcaag acgaagcggg actattttta tgggagacat tacgtaataa
    46921 tttctattac tctgctatca atgtacctaa agctaccgat gatttccgag acatagaccg
    46981 tgcgcttgtc tgggggttca actggaaact tggtccattc caattatggg atgcaatggg
    47041 atacgaacgt gttaaaacac gtatggaaga cgaacttgga gacttaccac aatggattag
    47101 tgatttagat ggtggctttt ataaacaaga tgagaccatt gaatatgcaa cacctatttc
    47161 tcacttcgta aaagatgaac tttgggataa aggtgatgcc aaactttccg taactcatga
    47221 tgatcaactg ttactgaaat tacaaagtaa aaataatgtc attaccgatg aattcaacga
    47281 tgcgttagtt gatgcgattg atttactgga aaatgaccat tacacaagta tggttattta
    47341 tgcagatggt aacaatttca gtgtgggtgc taaccttttc ttaatgaaaa aggcgcatga
    47401 agacggtctt gtagatgatg tcgttgcaca atcaattgat aaattacatt atagctttaa
    47461 tcgtttgaag tatagtttga aaccagtagt cacagctgtt caaggtcgtg ccttaggcgg
    47521 tggctgtgag cttgtacttt actcacctat tgttgtcgct gcaagtgaaa catatatcgg
    47581 tcttgttgaa gcaggtgttg gcttattacc gagtggcggt ggccttgcag aaatggctga
    47641 tcgcatatta cgcacatcgc ataagtttga tgacaaacaa gcttccatga caaaagtact
    47701 gacgaatatc gcatttgcga aagtctctac aaatgccttt gaggcacgtc gttatggtta
    47761 tttacgtgat acagatacga ttattttcaa tacagcacaa cgtgtcgaag ttgcgctcaa
    47821 acgtgcgaaa tatgaagcag aaacaaacta tattccgaat cctagacatc aatatatcgc
    47881 tttaggtgaa gacttcaaag cattgatcca aggacaatta gatgcgcaaa gacggggtca
    47941 ttttattagc gaccatgatt atcatattgc cttaaatatc gccacaattt tagcgggtgg
    48001 tgatttacca agaaatacat ttatcaatca acgttacatt caatcgttgg agaaaattgg
    48061 ctttattgac ttactaaaat ctaaaaaatc atatgaaaga attgcacata tgttaaaaac
    48121 tggtaagcca ttacgtaatt aaaagatagt cattaagaga ggatgataac catgcaagaa
    48181 gcatacattg tagcttatgg gcgttcagcc gcagcgaaag caaagcaagg cgcattattc
    48241 cacgaaagac ctgatgatgt cgcagccaaa gtattacaag gcgtattgaa acgtattgac
    48301 ggaaaattca ataagaatat gattgaagat gtcattgttg gtacggcttt tccagaagga
    48361 ttacaaggcc aaaacattgc acgaacgatt gcattgcgtg cgggattatc tgacacggta
    48421 ccgggtcaaa cagtgaatcg ctactgctca tcaggattac aaaccatcgc gattgcagcc
    48481 aatcaaatta tggctggtca aggagatata cttgtagctg gtggcgttga attgatgagt
    48541 gccgtaccaa tgggtggcaa cgagcccaca aacaatccaa ccttacaata tgatgatata
    48601 ggtgcgtcat atcctatggg tttaactgct gaaaatgtag catcccaatt tgacgtatca
    48661 cgtgaagatc aagatgctta tgctgtcaga agtcatcaac gtgcctatga cgcacaacgt
    48721 gatggtcggt tcaaagatga aattattcca atacaagtaa actcagttga atatacaaac
    48781 gcaggaccaa aagtacacac aaatatcttt gaccaagatg aatttatacg ccctgacacc
    48841 acgatggagg cattagccaa attacgtaca gtatttaaag ctgacggcac tgtgactgca
    48901 ggaacatctg ccccactttc tgatggtgca ggatttgtag ttttaatgtc tggagataaa
    48961 gtgaaagaac tcggcgtgac acctattgca cgattcgttg gttttaaggc agtaggcgtt
    49021 gacccgaaaa ttatgggtat tgggcctgca tatgcgattc ctgaagtatt gtcactcagc
    49081 aatctatctg ttgaagacat tgatttgatc gaattgaacg aagcatttgc ttctcaaacg
    49141 attgcatcta ttaaagaagt aggtctagat atatcacgta cgaatgtgaa tggtggcgct
    49201 attgctttag gtcatccatt aggtgctaca ggcgcaatgt taaccgcgcg tttacttaat
    49261 gaaatgggta gacgtcccga tagccgttac ggcatggtta cgatgtgtat tggtgtcggc
    49321 atgggtgcag ctgctatatt tgaatatgtg cgttagaatg gttgattttg gatgaagcgg
    49381 attcgttttg ttattgaatg aagtaggctg aagttgaagc cagttgaagt tgaagcgggt
    49441 tgaagcaatt tcgttttatt aatgaagctg tgtgaaatat agtgattgaa caaaaaagtg
    49501 gtttaatggg atggtggtta tttccgtttt agaatttaac atttacacgt ctaattttaa
    49561 tcattgtttt aaattttatg aatcgaagcc ctttgattta ataatatttg ctaatgctag
    49621 taacttatct gattgttcat gtttaaaata aagaaaacca ctcacatcag tgtgtgttcg
    49681 aactagactt gtaagttcca gttcggcacg actttctaaa gcaattatta ttgctgtgat
    49741 tgtcgtatat cacttagatg tgcgtggttt attttaatag gttagtaata tattaggtca
    49801 tgttatgttt aagactataa tgaataaata atttagaaat atgcttccga ttgttcgatg
    49861 ctttaattca gttagaagca tcatagaatg catgattact gttgtaaaga tacgtaatgt
    49921 tttgtattga ctgtatgtct ttggatagag ttacaaactt attttgttac tctaggccca
    49981 tatgtcgcag taccatctgc atgtgttgtt acattgtatg catttgtttt acttggcttc
    50041 ttgtatgtcg ggcgagctcc gtatgacact tgaccgtttg catgtgttgt tacgttgtat
    50101 gcatttgttt tgcttggctt gttttgtgtt gggcgagcgc catatgatac ttggccgttt
    50161 ccatgtgttg ttacgttata tgcgtttgtt ttgcttggct tgttttgtgt cggacgagct
    50221 ccgtatgata cttgaccatt tgcatgtgtt gttacgttat atgcatttgt ttctgatggc
    50281 ttattgaatc ttggtctcgc ttcatatcca aatgttccat cgttgtattc acggatacct
    50341 gtaccagcat ctctatattt aacatattta ggtgttttgt taaattgcgg tctcggacca
    50401 tattgagaag cttctgttgt ttcagttgct tgaggtttaa cttcaatatc acttgattct
    50461 ccttgagtac cttttaacgt tgattcagta ccttgtggtt ttatttcaag tttagatgag
    50521 ctaccttcaa gaccttctaa aatagggttc gttaacggtg ggtttgtata attattgctt
    50581 aatgatgggc cgctttgttc cattgttaga aaatcgggac cttgaacgat ttcaccttgt
    50641 accgttttat tttccatcgt tggatattcc ggacctttta caatttcacc tgtaattgtg
    50701 ccctgtggaa ttttaactaa tggttgtgca actggttgtg ttgtttcttc agctttacca
    50761 gccgtagttt taacctcttg ttggttatca actttaggtg cttgaggttc ttcaactttc
    50821 ttctcttctt ttactactgg cgattttgtt tcagtttctc cgtatttttt gacagttttc
    50881 tttttccaag aatcatctgc ttctttaact gcttttttcg tttcttcaac taatttatca
    50941 aaattaggtt tattatcact atttgtttta tagttatgtg ttgtaggatt atatttcgtt
    51001 atagatttcg gtctattttg tttagtttcc ataaagaaat catcaataat tgaatttaag
    51061 tcatcaatca tttctttttt aatacgttca tttgtaattt tatgtggatt gtctgtatct
    51121 ccaaggatta agtccagttt tgctcgtaac tctttcgcgt gctccccata atccttatca
    51181 ccataatatg atacaactaa tgtatcaatt tcagatacga gatcgtatac ttccttagtt
    51241 gctttatctt cttctgctgc attaaaagtt ttcaagtctg aattcttatc cttaatatct
    51301 ttaacttctc tgtgaaaatc atccagtgct ctctttaatg catcctgtag ttcattgtat
    51361 tctttcatcg aaagttcttc taaattatat ttatgaaaat tagccatttt taaatctgta
    51421 cgaggatttt cttttttata atttgcatac cattgtttat aatcttcata ttgagatttc
    51481 tttctctcca aaagatattg atcttccctt aatacctttt ccaacaacct atctttagct
    51541 tctttataaa tattatctcc atattcatat ttattagtta atcctatagc ataaattata
    51601 tagtcttcca aataatatag agctgaattt aaatatctgc tatctattaa tgtcccattt
    51661 ttactcccag cattaacttg tgatttccca ctataatcct ttgttactat cgcatctgct
    51721 ttgttatccc atgtaaataa gctagatgca actgctaatg cgcctagcga aattatttgc
    51781 tttttcataa ttttttaatt cctccaaaat gtaattgccc aatctacatt aaagaaacaa
    51841 aatattaaaa gacattaact atatattaac tagaataaac aaagcattaa ctatcttttt
    51901 gttatagtta attagctttg caatatacaa tagtgtataa tttttttgat taatgtacat
    51961 caaaggagta acaaagcatg acaacacaaa tgaaaatcaa aacatattta gttgctggta
    52021 ttaaagcggc gctccttgat acgactggta ttaaattagc aagcaaatct gaaactacat
    52081 cacatacgta tcaacatcaa gcgcttgtag atcaattaca tgaattaata gcaaacactg
    52141 acttaaataa attatcgtac ctaaatttag atgcgtttca aaaacgcgat attttagctg
    52201 cgcactatat tgcaaaatcc gctatacgca ctaaaaattt ggatcaaatg actaaagcga
    52261 aacaaagatt agaaagtatt tacaattcaa tttctaaccc tttgcattca caaaacaatt
    52321 aataattcac caataaatca tgtaagtgtt tgtgacgcca aattgccata caaatacttg
    52381 tcatatgaat ataaacgaat gaaacgattg cctatcccat agatggcaac attaaaaaga
    52441 cctctgaagg tatcatcttg ataactttag aggtctttgt ttatattatt caaacaaaat
    52501 tcttatagaa cgatcgaagt atgtttcgtc tttcttttct tctttaatct gatcagctaa
    52561 tgctgggtca tctgtgataa taccatcaac attggtttgt aagtatttcg ttaaatcttc
    52621 ttcgccgtta atagtccaag tatagacttc tttattttcc aagtgcgctt gattaacaag
    52681 tcttggcgaa taagaaaaat cttcgatgac aaagaaatct aatgatgttt ctttaaaatg
    52741 accaaactgc aacggaatga tataaccaca cttgagatat ggcgcttctt ttttcaactt
    52801 agtcatcaca tcataatcca aagacatcac acgatattga tgttcaacac catgcttttt
    52861 caaaatatca ataacacgtt gtgtataatc tgctggttct ttaccatgtg gctttaactc
    52921 tactagtagc ttcacatttg attgtttagc cgtttcaata aattcgtcta aggatacaaa
    52981 ttttgcttca tgtccatttt gacgcatttt caaaccgacg atatctttga aattagattc
    53041 agaaatattt ttattaacac ctgttaaacg tttcaaattg ttatcatgac taacaacaaa
    53101 ttgtttatct ttcgtcataa ttgtatctaa ctcaacgtat tcgacattcg cttttgcagc
    53161 agctttcaat gacggaatag aattttcaac gcctttatct tcgaaaccac gatggccaat
    53221 aatggagata tttgtattga tagtattatt gtaaagtaag tacatgttat aaccgataaa
    53281 acatgtcact gcaagcacca ttgaaattat aaagaaccta gacttccgtt tcggttttgg
    53341 atatttaaat tctaagcccg gttggtctaa tacattctct tgttttaagt gcagtactaa
    53401 cacactgatt aatgataatt tcgtaaataa ataatagaag aacaatgcgc ttttcaatac
    53461 aacaaataaa attgatgaga ctaaaaactt atctccttct tcatctacac aaatagcaag
    53521 atatgttgct cctgaaataa ttaatgttaa aatcgcacca atgatgagtt ctaatataac
    53581 tatttctata acaagccgaa acttatttcg cttcgtaatt tgccaactta gtctcatatt
    53641 tttaaataac gactggcggt ttaaaatcgt taacggtaga gtaaatatta atttaaaatt
    53701 taatataaat acagcaatca taaaggtacc gtaaatgatt atacctttcg tcgttttcat
    53761 aagttcttcc gttaaaaatt taggtatgta aatatttttt gttaatactg aacttagtcc
    53821 taggttggca atgggtatca ttaacattaa ataaatgaca aagaaaataa ctggtacacc
    53881 tatgagttta cgcacattta caaaggcatt tttaaaaatg gatttaaatg taataatctg
    53941 tcgatcaaag ccggcataaa ccatataaac taacaatgaa aactctacat aaatcagaaa
    54001 ggcaacactt aatatgaata taataagaag tatcacactg gcgggatgac taacgatttc
    54061 cgtccaattg ttaatcgtaa gttggctttg cccagctact tttaacatca tattaaatag
    54121 taaaattaag tatgtactac taataaaaat catgattaac tgcaatagta aggcattaat
    54181 gctaaaacgc cctttatttt gatacagtaa tttaaatact gcccatatat ctttactaat
    54241 tctcttcata atcacgctcc gcattgcttt aatattaagt ttcatcttaa tatttttcat
    54301 tactcagggt caataaaaat ttgaaaagac tcatattcat atgcaagtag caaataataa
    54361 cccattcaac atcagctaaa tgatgatatt ggaaccccac ctttaacaag acatcacatt
    54421 ctttatcagc atagctactt acaaaaacgt cttccttcaa ccattgtaga agttgtctca
    54481 tatacttact acgtcttgct aatactcaat actcaattga aaaagaagca tatgcccctt
    54541 cactcttgaa gttgcatatg ctttcttttc ggtctgaatt gtatttataa ttcaacggga
    54601 attttccctt tgaagttaac ataacggtag gctgctttaa cagcttcatc atcgggcgct
    54661 tcgacatctt ctaattcata tgcaatgccc aatgttttcc acttatgaac acctaactga
    54721 tgatatggca gaatttcaaa cttttcgacg ttatcaagag aattaataaa ttcccctagt
    54781 ttaattaaat cgtctttatc atcagaataa ccaggcacaa ggacatgtcg aatccataca
    54841 ggttgtttca tatctgacag tttgcgcgcg aagttaagga tgtgtgtatt aggctttcct
    54901 gtcaatctaa tatgtttgtc attatcaata tgttttatat ctaataatat caagtctgta
    54961 tgtttttgta attcttcaaa atgcctttga aatgcttttg tatcattagc acatccagcc
    55021 gatgtgtcta agcaagtgtg cacaccattt tcttttaatt ctgcaaataa tttttctaag
    55081 aatggcattt gtaacaatgg ttcgccacca ctgactgtta caccgccacc cgatgcatca
    55141 aagtatggtt tgtatggtaa tatttcattc accatttcat caactgtgac ttctcttgat
    55201 ggctcactaa ttttccaagt atctggattg tggcaataca agcatctaag taagcatcct
    55261 tgtgtaaata atatatatct taatcccggt ccatcgacag tacctaaact ttcgacagaa
    55321 tgtaagtgtc ccttaagcat agtgctccca ccttaaattt tgttacatac tttcatggaa
    55381 tgtacgagaa attacatcta attgttgttc acgtgttaat ttaatgaagt taacagcgta
    55441 accagataca cggattgtta actgtggata ttcttctgga tgttccattg catctattaa
    55501 tgtttcacgg ttaaatacgt taatatttaa gtggtgacca cattgcattg cgtaaccatc
    55561 taacatacta gttaagttac ggttttgatc ttctggttct ttacctaatg attttggtac
    55621 gatactgaat gtatttgaaa taccatcttt acagcaatcg taagggatct tagctacaga
    55681 acttaatgaa gataatgcac ctttttggtc acggccatgc attgggtttg cacctggagc
    55741 aaatggttcg ccagctttac gtccgtctgg tgtgttacca gttttcttac cgtatacaac
    55801 gtttgaagta attgttaata cactcattgt atgttctgaa tcacgatatg ttttatgact
    55861 acgtaattta gtcatgaagc gttctactaa atcaactgca atatcatcta cacggtcgtc
    55921 attgttaccg tatttaggga agtcgccttc gatttcaaag tctactacaa gaccttcttc
    55981 gttacgaatt ggtttaactt gtgcatattt aattgcagat aatgagtcag ctgctactga
    56041 taaaccagcg atacctgttg ccattgtacg tacaatttct gtatcatgta atgccatttc
    56101 aatacgttca tagctgtatt tatcgtgcat gtagtgaata acatttaatg agttaatgta
    56161 aacacctgct agccaatcca tcatttgatc aaatttcttg aatacttcgt catattctaa
    56221 tacttcgctg ttaatacctt cgaagtttgg accaacttgt gcaccagatt tttcatcttt
    56281 accaccattg atagcgtaaa gtaatgtttt agctaagttc gcacgtgcac cgaagaattg
    56341 catttgttta ccaattgtca tcgctgatac acaacatgcg ataccatagt catcgccata
    56401 gctttcacgc ataatgtcat cattttcata ttggatagaa cttgttttaa tactcatttt
    56461 tgcacagtat gttttgaagt tgtcaggtaa acgtactgac cataatactg ttaagtttgg
    56521 ttctggagct ggacctaagt tatctaatga gtgtaagaaa cggaatgagt ttttcgtaac
    56581 aagtggacgt ccgtcaatac ctacaccacc gatagattca gttacccaag ttgggtctcc
    56641 agagaataat tcattgtaat caggtgtacg agcaaattta acaatacgta atttcatgat
    56701 gaagtggtca ataatttctt gaacttcgct ttcagtaata acgcctgctt taaggtcacg
    56761 ttcagcatag atatctaaga atgttgatgt acgacctaaa ctcattgctg caccgttttg
    56821 ttctttaatt gcagcaaggt atgctaagta taaccattga actgcttctt tgaagttttc
    56881 tgctggacgg cttaaatcga aaccatattt ttgtccaagt tcttttaatt cttttaatgc
    56941 acgatattgt tctgataatt cttcacgtaa acgaattaca tcttctgaca tttctgtaga
    57001 catcgtgttg aagtcgtgca ttttttcttc cattaagaaa tctacaccat ataaagctac
    57061 acgacgatag tcaccgataa tacgtccacg tccgtatgca tcaggtaaac cagtgattac
    57121 acctgcttta cggcagttca acatttctct agaatatgca tcgaatacac cttggttatg
    57181 tgttttacga taatctgtaa agattttttc agtttcttcg tctaattcgt aaccgtaagc
    57241 ttcacaagct gctttcgcca tacgaatacc accgaatggt tgcattgaac gtttgaatgg
    57301 cttttcagtt tgtacaccta caattgtttc taaatctttg tctaaataac cagcatcatg
    57361 agatgtgatt gttgaagcta ctttcgtgtc catatcccac atgccgccac gttcacgttc
    57421 ttctttcgat aactgcatta cttgttccca aagtttagaa gttgcttctg ttggtcctgc
    57481 taaaaatgaa tcattacctt cataaagagt gtagtttaat tggataaact ctcttacatc
    57541 tacgtgtctg ttccatcttc catttttaaa tccttgccaa gctgttgcat gatttttatt
    57601 tgtttctaac atactaaacg cctccatctg tataatgttg tgaatttgaa atgctatgtt
    57661 aacgttttca tagtcattgt agcatgtttg ttgtgatttt tttcacataa attatgtaca
    57721 attttcatca aatgtatgtc aaaacataga aactgtacta atttgtgtaa cagtaatatg
    57781 ctaattgcat tctgttacac aacagttata atgttcacta ttttcattgc tttaaaagtt
    57841 aatttaggag tatatatttg tttataaaaa atgattgttg ataagcgtta aatcaacact
    57901 tagaaagatt tacttttata tatgaagaga ctggattaaa tacttttatt gacgtaaaaa
    57961 ttcacttttg aaccgttcaa tatcttgccg atttttatat aacagctaca aataaaatat
    58021 aacagtttga ttttacagcc tcggtaaatc gtcttgacaa acaaaaattt tgtgctatca
    58081 caacatttgc aacgtcttaa caagtcatct ataaacattt ctaaatattt aacattactt
    58141 atgcgtcatt tattgctaaa attattgtat taaaatatac atagaattga tgggatatca
    58201 tgaaatcaaa aatttatatc ttgctattat ttctcatttt tttatcagca tgcgctaata
    58261 cgcgtcactc tgaatccgat aaaaatgtat taacagttta ttctccgtat caatcaaact
    58321 tgattcgtcc aattttaaat gaatttgaaa aacaagagca tgtcaaaatt gaaattaaac
    58381 acggatctac tcaagtactg ctttcaaact tgcataacga agatttttcg gagcgtggtg
    58441 atgtctttat gggtggtgtg ttgtcagaaa caattgatca tccagaagat tttgttccct
    58501 atcaagatac atctgtaaca cagcaattag aggattatcg ctcgaacaat aaatatgtta
    58561 ctagttttct attaatgcca acagttatag tagtgaattc agatttacaa ggagatatta
    58621 agattcgagg ttatcaagat ttattacaac ctatacttaa aggtaaaatt gcgtactcaa
    58681 atccaaatac aacaacgaca ggctatcaac atatgcgtgc tatttatagc atgcatcatc
    58741 gagtaagtga tgtgcatcaa ttccaaaacc atgcgatgca actgtcaaag acgtctaaag
    58801 tcattgaaga tgttgcaaaa ggtaaatatt acgcaggtct aagctacgaa caagatgcac
    58861 gcacatggaa aaacaaaggt tatcctgtat caatcgttta tccaattgaa ggaacaatgt
    58921 taaatgttga tggtattgct ttagttaaaa atgcacaccc tcatcctaag cgtaaaaagt
    58981 tagtgcaata tttaacaagc cgctcagtac aacaacgatt agttgcagag ttcgatgcca
    59041 agtcaattcg aaaggatgtt agtgaacaaa gcgatcagtc tatcgaaaat cttaagaaca
    59101 tacctttaat accgaaatct aaattaccgg atattccaca tcataaattt ttggagatga
    59161 ttcaatgacg gcatacaaac cttatagaca tcaattaaga cgatcgttat ttgcgtctac
    59221 tatttttcca gtatttttag tcattattat tggactagta agcttctatg ccatctatat
    59281 ttggattgag catcgtacga ttcatcaaca tgttgatgaa agtcagtcat ctttacatca
    59341 tactgaaaag cagattcaga catttattac acagcataat aattcgtttc aagagctcga
    59401 tttaacaaat catcatgatg tgaccgctac aaaacgtgaa ttattaaaat taatacacca
    59461 acagcccgca acactttatt atgaattatc tggaccaaat cagtttataa ccaataatta
    59521 tgaacattta aacactaaaa atatgtattt attttcaacg catcagctta aattcaaaaa
    59581 tagtacatat atgcttaaaa tttatatggc taacacccca cgtttaagtg aaattaagaa
    59641 agataaccga caattcgcac ttatcgttga tcaatatgac aatattttat acgccaacga
    59701 tgaccgtttt actataggtg aaaagtatag accacaacaa ttcggattta tgaatgaatc
    59761 tgtaaaatta aaccatgctg accatcgtct cattatttat aaagatatac atgaaaacat
    59821 tgaagatggt atcacgttgt taattgttat ggccgtagtt cttgttttac tagtaatatt
    59881 tggtttcatt agcgcagata atatggctaa acgacaaaca aaagatatcg aaacgattat
    59941 tcaaaaaatt tactatgcca aaaatcgtca tctaggtaca tatacgcctt taaaaaacaa
    60001 cagtgaacta gaagaaatta ataattatat ttacgattta tttgaatcaa atgagcaact
    60061 gatccattct atcgagcata cagaacgtcg cttgcgtgat attcagttaa aggaaataga
    60121 acgccaattc cagcctcatt tcctattcaa tacgatgcaa acaatacaat atttaattac
    60181 gttatctccg aaattagcac aaactgtggt ccaacaatta tcgcaaatgt tacgttactc
    60241 attacgtaca aactcacata cagtggaatt aaacgaagag ttgaactaca ttgaacagta
    60301 tgttgcaata caaaacatcc gttttgatga catgattaag ctccatattg aaagttctga
    60361 agaagcgcgt caccagacaa ttggtaaaat gatgcttcag ccactcatag aaaatgctat
    60421 taaacatggt agagatactg agtcattgga cattactata cgcctgacat tggcacgtca
    60481 gaatttacat gttttagttt gcgacaatgg tatcgggatg tcgtcatctc gcctgcaata
    60541 tgtacgccaa tctcttaata atgatgtatt tgatactaag catttgggat taaatcattt
    60601 acacaataaa gcaatgattc aatatggttc acatgcacga ctccatattt tttcaaagcg
    60661 taatcaaggt acgttaattt gttataaaat accactttca agggggaatg tagatgttta
    60721 aggtagttat ttgtgatgat gaacgaatta ttcgagaagg attaaagcaa atcattcctt
    60781 ggggagacta tcatttcaat acaatataca ctgctaaaga tggtgttgaa gcattatcac
    60841 taattcaaca acatcagcca gaattagtaa ttactgatat tcgtatgcca cgtaaaaatg
    60901 gcgtcgattt actcaatgat attgcgcatc tagattgtaa tgtcatcata ttatcgagtt
    60961 acgatgactt tgaatatatg aaagccggca ttcaacatca tgttcttgat tatttattaa
    61021 aaccagtaga ccatgcacaa ttagaagtta ttttaggaag acttgtccgt accttattag
    61081 agcaacaatc acagaacggc cgtagcttag catcttgtca tgatgccttc caaccactat
    61141 taaaagtaga atacgatgat tattatgtaa accagattgt ggatcaaatt aagcaatctt
    61201 atcaaacgaa agtgactgtt tcagatttaa ttcaacatat cgatgttagt gaatcgtatg
    61261 cgatgcgaac atttaaagat catgttggta ttacaattgt cgattattta aatcgctatc
    61321 gtattttaca atctctgcaa ttgttagatc gacattacaa acactatgaa attgcagaca
    61381 aagtaggctt ttcagagtat aaaatgttta gctatcattt taaaaaatat ttacaaatgt
    61441 cgccaagtga ttattgtaag caagcaaaat agattagcac ataactaatg attatggcgc
    61501 ctccattttg ttctatgaaa aagcggactg atagagaagt tttcactttt ctttcagtcc
    61561 gctttttaga gagtttaaat ataaaacaat actccctaca atattgacac cgctatatgt
    61621 ctttattgta aggagtaaat tcattgagtc accctaagtc gggcaaataa ggatacttca
    61681 taacagggaa ttatgatctg ccccgagaca tagagatgat atatttgcaa gggtagaagc
    61741 agtatctttc atctcttcac gcacatgccg tcggggcagg attgccaact atattgcaat
    61801 agattggcga tacttttaat ccgatttata ttaaattttt aaacttctaa ttttcttttc
    61861 ttcatagaaa gcaacgattc ctaatagaat catgcctagg aataatgcaa catagaagac
    61921 gatgaaaaca tctgtccatc cacttaatgt atatccaaag atgtttaaac cgttacgtgt
    61981 tggatcagca atagccgcca aaccaacttt cgccattgag tcaccgaata gatacgcgaa
    62041 tgaacctgtc attccgtttg ctacactgat ggcattttta ggaacaaaac cagtcaatga
    62101 tacaccaatt aataattgcg gaccaaagat taacgcacct aatgcaaaca atgaaatgtt
    62161 aaccatcatg acacttgtag catttgtgta gaataagaca acaaatgtaa tcataaacat
    62221 acagccaata gctacaattg cacgacgacc ttttaataag tctgatacgt agccccataa
    62281 taaacttgca actaatgcac caatttcaaa gtagaatatc gtattaactg catcgccttt
    62341 actaaagtgt aaatgctctg acacatataa cggtgcccag ttatcaatac cgattcgtac
    62401 aatgtataca aagacgtttg aaacacatag aatccatata acaggatttc ccaggatata
    62461 ttttttaaag atctcccatt tcgtcatacc ttgagaatca atattttctt tatcgaccgg
    62521 ctcttcccaa atttcttcag cacgattcca tcctaattct tccggatcat cttttccgat
    62581 aaataatgtt gcgataccaa taagtaatgc aatcaccgat gggaaaatga acatccctat
    62641 aacatttcca tggaagaata cattagcacc ccaaagtgca acaccacctg ctatggcacc
    62701 accgatatta tgtgatgtat tccagaatcc taagtatcgg ccacgtttcg ttcttggcgc
    62761 ccatcttgaa atcgttgaat aacttgcagg tccaccaact gattggaaca ccccgttaag
    62821 tccccaaagt acaattaata atcccattac agaaccaaag taacttaaaa caaatcccat
    62881 aattaaaact gtaatcgcag ataagataag taagaacgag ataatacgtt ttgtgttacg
    62941 tccatcgaca aaatatccaa gtaatgtttt tcctaaaccg tacgtgatac taaatgctaa
    63001 tccgatataa ccaagttcta atgtagataa tccaatttcc tcttttaaaa acggttgtgc
    63061 cgccttaaag ttgtttcgaa ttaaatacat agccatataa acaaagaaca ctacgaagaa
    63121 agcttgcatg aagtttctaa gccataattt acgttgtacc gataatggaa tgcctttgtt
    63181 cggaatctta tggatatcaa aaaagttcat ctctgtcacc tcaatcattt tcgttaagcc
    63241 aaatatatca cgtcggtgta agcgcttaat atccttaaaa ttattaaatt atccaaaata
    63301 ctgaacataa acacatactt agaaacatat ttatgtacta aaattaataa atagttaata
    63361 gtatttgaca agctttatct ataaaatact ttatgttgtc aaaatcaacg cttatacata
    63421 tgacataatg acggaggaca tcatgattct atctatatta cttatctttt tctgtatcag
    63481 acttgtcagc ttaaagatat ctattaatca ctcaaaacaa ttaaaagcag acggtgcagt
    63541 tgaatatggc gttaaaaatt cgaagttttt agcaataaca cacgttttaa tttatgtatt
    63601 ggctggtgta gaggcattta ttaataaaga tacatttagc tttgcaaatg gtattggctt
    63661 agttatatta atctttgctt atatcatgtt atttatggtt attaaaactt taggtggtat
    63721 ttggacatta aaattattca ttttacctaa ccatcctatt attaaatctg gattatataa
    63781 aattacaaaa cacccaaatt acttcttaaa catcattcct gaattaatcg gtgtattatt
    63841 attaacacac gcaacatata caactatttt attagtaccg tatgcgtatt tcttatatgt
    63901 acgtattaaa caagaagaga aattaatgaa tatttaaaaa gatgaaagcc tgtcatcact
    63961 ttagtctaca tcgacgtcca tctttaaagt gcattctgaa atattatatt cagaaacctt
    64021 taaagtatgt cgtgtctatg accattgtga caacaggctt ttttttatta tcatatacct
    64081 cactattgaa aaggttatat actcttttca atggtgagga ttgttactgt gaatgggtat
    64141 gaccatcgct atatacttat ataaagtaca ccttctttac aaacaatgac ttgatttaag
    64201 gtgtgaaatt tttggcaatg gcttggcgac atatgcattt attcaacact gccaccagtc
    64261 ttacttcaga caaacttcag actaacctcg agttcgtatt atgcccacca catgtctgct
    64321 ggcttgtcgt aaatattaac agatttcaaa gtttgacaag ctttttggaa accttcttct
    64381 actgacataa taggatcttc atgttcaata cttaatacat aatcatatcc attaataata
    64441 agttgactta tgatatctgc ccatacatat ggactatgtc cataaccaac tgtacggaat
    64501 gtccatgctc ttgtcgcaac gttaccatat ggttgcatat cagttagacc atacatattt
    64561 acattttctt gattaatata cgtatcttta gcatggaagt gatgaattgc atttgcttgg
    64621 cctaatatgc gaatcgcagc aattgggtca ataccttgcc accatagatg actaggatct
    64681 aagttagcac cgatatattc atttgtagcc tcacgtaact tcaacattgt atatggtgta
    64741 tgcactaaaa atcctgcatg caactctatg gcaattttta catcttgctc ttttgcaaac
    64801 tcagctaaat cttgccaata tggtatcaac ttttcattcc actgataatc ataaatttca
    64861 gagtaggctg ttggccaagg tgtaacaggc caattaggct ttttagcggt atcatctgat
    64921 cctgcaatgc cagaaaatgt attaacaact ggcacgtcta atagatttgc taaacggatt
    64981 gttttacgta acgtttcatc ggcttctttc gcttctatcg gatctggaga aattggattg
    65041 ttatgacaac tgaaaccact tatttgtaag cctctgtctg tgattgactt cataaatgct
    65101 tggcgcttgt cttcattttc taacaactca tctaacttac aaaatttatc tcctgggtta
    65161 ccacctgttc caacttcaat catatccaat ccagattctg agacataatc taacatatct
    65221 tcaaaatttt tatcgtaaaa taatactgaa aatacaccta ttttcattga aaagcactcc
    65281 ttctttttat attgagaatt ttgtatcata tatgataatc attaaagttg tatgctcttg
    65341 ttatcaagat cgctacgata aatcgcttct ataagggcat ttacattgcg tgcttcttcc
    65401 ggtttcacca caatctcttc tatccctaaa cacgcattga caaagttacg cgcctgtctc
    65461 tcaccagcaa tgtcttcgtt atgctcaaca ttagctttgc tttcaaaaat agttccaaag
    65521 cggggctcat atatttcaaa tggaaataaa ttgataccgc catcttctcc tgataaacta
    65581 acgtgaacct tatcttcttt gatatttgca gaccacgaac attcaaactg catgcttgct
    65641 cgattggcaa atgtcatata actagtaaca tgatcatcga catcaaattt agtatgatca
    65701 aatgttcccc aatcattaat ttgattcggt tgtttgctca attgattata tgtttttcct
    65761 agcacttcat gcggcaccat atctttacct agtagccaca aagataagtc taacaagtgg
    65821 caaccataat cgattaaact accgccacct tgcaacgctt tattggtaaa aacaccccag
    65881 ccaggcactt tacgcctacg catcgcttgt acacgtgcta ctaaaggttt accaaccaca
    65941 cctgattcaa ttgctttttt agcagtaatt gccacatctg tgtgacgata atgatatgcg
    66001 acagttaata atttgtgatt tttattagcc gcttcaatca tgcgatcaca ctcttccgtc
    66061 gtcatcgcca ttggcttttc acacaataca tggacaccat ggttcaatgc ttctatagaa
    66121 agatcagcat ggaatttatt aggtgtacaa atgaccaccg catcaacaag tttaaacagc
    66181 tcgctaggtg tctcaactgc atgaggtata ttaaagcgct tcgcaacatc aatcatctgc
    66241 actgtattaa tatcttgtac tgcaactaat gagactgtgt ctttgagttt cagcaatgct
    66301 ggaatatgac ggtcttgtgc aataccacca acacctatca caccaacttt taattttgtc
    66361 atgatgtgcc tccttaccgt atgatgttat tcaaagtaaa ttgctttgcc tgattttgca
    66421 gactgataaa tcgcttcaag aatttttgta actaccattg cttgttccgg tttcacaact
    66481 ggttcagtat catttacaac tgcatcaatc caagcttttg cttcttcttc agcttcatcc
    66541 acttcattac cttcataaaa gtcgacgcct ttgttttcca attcaacgtg tttggtataa
    66601 agtgtaccca tgtcttcacc atgaatacgt agaccatctt tcatatcagc acctgcttta
    66661 gttcctgata atgaacattt tgcctcatcc acttctaaag aattaatcgc ccaagcggat
    66721 tctaaaatga tcgtcgctcc attcttcatt ttaataaatc caaacgcaga atcttcaact
    66781 gtaaattcat ctggattcca tgaaccccaa gcgtttgccg catgatgctg tttatttaat
    66841 ttatggaatg ttgaacccat cactgattct ggttcataat tatccatcat ccataacgtt
    66901 aaatctaaag cgtgtgtacc gatatcgatt aatggtcctc caccttgagc ttcttcgtct
    66961 agaaagacac cccatgttgg tactgctcga cgacgaatgg catgtgcctt tccgaagtaa
    67021 atgtctccta agtcgccacg ttgcgctgct tgatgtaaaa attgactatc tgctcggaaa
    67081 cgattttgat aacctattgt taatttttta cctgttgatt tagctgtatc tatcattttt
    67141 tgagcttctg ctgtcgtttt agccattggt ttttcacaca tcacatgttt accagcatgc
    67201 aaccctgcta cagtaatttc acaatgcgag tcatttggcg tacaaacatg gataacatca
    67261 atcgtgtcat cttttaacaa tgctttgtaa tcatcataaa cctttgcatt gtcagttccg
    67321 tatgcttctg ccgcactcgc tgctttcgaa atgtctacgt cacaaaatgc gatcatttca
    67381 acattttcaa ctttttgtaa acttggcatg tgcttgccat tcgcaatacc accacaccca
    67441 atgattccaa ctttaatcgt catcataacg tcctcctttt aatctttatc gtcattagtg
    67501 gttctttatc aattctgccc cactcctaat ttcaaactaa ccttttgtcg cacctgttgt
    67561 taaacctgat actaaatagc gttgcaagaa caagaatacg attgctatag gtactgcaat
    67621 cataattgcc cctgctgcaa acactgtgaa attatttgca tacttatcat taataaagtt
    67681 gaacaatcca actgctaatg tgaatttttc aggacttctt aatagtattt taggtaatat
    67741 aaagtccata aatggcccca taaaattgaa caaagcaaca actgctaaaa tcggcttagc
    67801 taatggaagc ataatttgta agaaaatacg catatgccct gcaccatcaa ttttggcaga
    67861 ttcatcaagt tcttttggaa tcgtatcgaa gtaacctttc actaaaaagg cattcatcgg
    67921 tattgatcca ccaatatata ccagtgttag tccaaataaa gaatctaata atccaattgt
    67981 atttagcaaa atatagattg cgaccattgc cattaataca gggaacattt gtaaaatcaa
    68041 aaatgtaatc agcccgtatt tacgaccaac aaagcgatat ctagaaaaag catatgctgt
    68101 taacgtgaca aatatcacac taaacagtgc atttgcagat gctacgataa gcgtattttt
    68161 ataccaagtc aggtattgac tactgtcatc gaatagtaag aatgcataat ttttaaatgt
    68221 tgcattgtct ggtatcattt tggcaccata caagttcgta cctggattaa gggaaatgcc
    68281 aaatgtccac agtagtggat ataaaatgat gacaaacatc atcgctataa aactgtaaat
    68341 accgattgct tttaatatgt ttttcttctt tgtcatctta taaacctccc tcatctttaa
    68401 acgtacttgt acgtctgaat tgaataaatg cgacaatagc aacaataaat ccaataatta
    68461 atgacacaac tgcacccatg ttgaagttgt taaactcaaa tgtcagatta tacacccaag
    68521 atatcaagat atctgtacta ccagcattct gccctgacac tggtggaccg cctttattaa
    68581 atagataaat aagattaaaa ttattgaaat tacctgcata ttgcataatt aacaatggcg
    68641 ctgtggcgta aatgacatgc gggaatgtga tgtttctaaa cttttgccaa ctagacgcac
    68701 catccatatc tgctgcttcg taccaatctg atgaaatact ttgcagtact ccagtgaaca
    68761 gtgcaaagac aaatgggaat ccaagccata cttgaatgcc gattaatgcc acttttgccc
    68821 aaaacggatc acttaaccat gctggtgcta cacctaataa aggttgcaaa atatcattat
    68881 ttatcgcacc aaattcatca ttaaataacg ctacaaatat taaaattgtc acaaatgatg
    68941 gtacagccca aggtaggatt aacacagtac ggataaattt cttacctttg acgacagggt
    69001 gatttacaat aattgccaaa aacagcccta atgcaatttg aagtgtcgtt gcaacaagcg
    69061 tccatactaa tgtccaagta ataacactga aaaatgtttt acgccaaacg ccaattgtga
    69121 ataacgtttt aaagttatct aaaccaaccc attctaatgt gtgtctcgga ggcgcgttgt
    69181 ataaattgta atttgtaaat gctactccaa acataaatat taatggaaat acaactacaa
    69241 atatcaataa gaatgtacct ggtgagatta gtaagtatgg gaacgtcttg tcccatgttg
    69301 ccaccatacg cgccttcgga tcctttattt cctcattgcg attaaatcgt tcagcattac
    69361 gatatgcatc taaaatatta atgatatata gcatgattgc gaaagcaacg agtaagatag
    69421 aaataatacc ttgtgcaagt aagacacgag aatcgtctaa cttaggtact gtccctaatg
    69481 tgaacaatcc ccaaaaacca atatttaaaa agctataaaa aacagaaata aaactgatga
    69541 aaaagataaa aaatatcgtc cctttaatgg gtcttttatt ataaaactgt cccaaaccag
    69601 gtataacaga taataatgcc gctaatttag ggttacgttt cgtcataact aatctccttt
    69661 cttatcattt tgtgatggat gaagaatctt aatattttgc gttatatcat tcgtcgcctc
    69721 atctaacgct tgtttaggat tcttaccatt tgaaataaat atgcttgcat tgcccatcgg
    69781 ttcccaaact tgtcgcattt caggaatatt aggcatcggt tcagcatgac gtgcttgctt
    69841 ttcaaacact tttaaatttg gattagatga tttcacgtca acacgtccag taatttcgct
    69901 catttcatct gtatattttt gtaatgtatc tttactagtg atatacagca ttaaatcttt
    69961 agcccaatac ttatgtttac tatattcaga taaataccaa ccacgtacac ctagaaatgg
    70021 tttcataggt ttgccaccat ctgtaggtaa tgttgttact cctaaatctt taccaaacgt
    70081 ttcttgatat tcattaatgt tccacggtcc agtgacaaat tgtcctactt ttccttcttt
    70141 aaaaagacca atcatgacat catgtgttgc tgccttagga agataccctt tgtcgtacca
    70201 tttttgtaat cgttcagcat tcttgacgac atgttttgaa tttagtccta gctgatgaat
    70261 atcatattca ctgccatttt tcttgaaaat ataatcatca ttgccgaata aaaacggata
    70321 attaaaatag aaatttttag catcaaataa cataccgtat tgtttctttt tactatcagt
    70381 tagtttggca gcattagctt caacttcttc taacgtttgc ggtgcatttt tcactaattt
    70441 tttattataa aaaagtgcgg ttgtttcaac gatagctggc aatgctagtt gcttattgtc
    70501 ataattcatc gctttaagtg cttgcttatt gaaacctttc aactcatctt ttgataattt
    70561 gatttcagca gctaagcctt gtagataggc acttccagta ttatcatgtg ctaagaaaaa
    70621 gatatctgga ccttttcctg caggagcgtc tagcgaaata ttttctagtt gatcattttg
    70681 accaatattt acaagcttta ctttgatgcc agttttttta gtatattgat ccgtaatttt
    70741 tttataaaac gccatttgct tgtcgccatc cacccacatc gtaagttggt taggcttgtc
    70801 tttagaatta tctttattca atgctttatc aatatcttct ttcgaacgat tagggccaca
    70861 tgcagttacg attaataaca ttaccacggc taacgtgata cattttaaaa ttttagacat
    70921 gaatactccc ccttagacga tacgatttcc tgttttttca tcaaaaaagt gacacttatt
    70981 catatcaaat gctagtgtaa tcttgtcgtt cgccatcact tgagttcttg aatctaattt
    71041 agaaatcaat tccattcctt ggaatgtgct atgtaccata atttctgaac ctaacagttc
    71101 ggatacaact acttcagatt caaattgtgt ctctggagaa gtttgaataa atattggttc
    71161 ttcatgaatg tcttcagctc gaatacctaa aataatttcc ttgtctaagt agccagcagc
    71221 ttttaacttt tcaaattttt tattatgtaa tttaaaatgg tgtgttccta ccttcaatcc
    71281 gtccatttca actgtagcat tcaacatatt cattgctggt gagccgataa attgcgccac
    71341 aaatatgcaa ttaggggcat catatatttc tctaggtgtg ccgacttgca taatgtcgcc
    71401 atctttcaaa acaacaattc gactagccat cgtcaatgct tcagtttgat catgtgtaac
    71461 ataaattgtc gtagtattaa gtcgcttatg taatttcaat atttctgtgc gcatttgtac
    71521 tcgaagcttc gcatcaagat tcgataatgg ttcatccatt aaaaagactt tcgcatccct
    71581 aacaatagct ctgcccaaag caacacgttg acgctgtccg ccagataacg cttttggttt
    71641 acgaccaaga tactcagtta atcctaatat ttcagctgct tcattaactt tttgttcaat
    71701 ctctttttta tttactttac gtagctttag cccaaatgcc atattttcaa aaacagtcat
    71761 atgtggatat aatgcatagt tttgaaatac catcgcaata tctctattct ttggttcaac
    71821 atcgttcatg cgttccccat caatataaaa atctccagat gtgatagact ctagtccagc
    71881 aaccattcgt aatgttgttg atttaccaca tcccgatggt ccaacaaata caatgaattc
    71941 tttgtcagta atatgtagat taaaatcttt cactacagta ttgttgttat catacgtctt
    72001 tttaatatgc tctaacttta gttctgccat gttatgcctc ccatactttg tttacagttt
    72061 gattgtgcaa tacacttcgt gaaaacgttt gcacaaatac ttacataaat catatattca
    72121 gtcattatta atgtcaatca taaattataa acgtttacat tactgtcaga attaaacttt
    72181 tatacagaaa attgatatag tgcacaacta ttttctagtg tgacaataat gttttttatg
    72241 tgcgatattt cactgccaat gctaacaacc ttgtcttaca ttttaagtaa tatctaaatc
    72301 aaacaagtat gacttagcat tacgattttc attgaaaaaa gcaaccatta tgtaagtatc
    72361 atactcacac aatgattgct taatttttta gactgcgggc acccttctat aactacaaat
    72421 catataggct acgttcccca tcaggtaact gatcgataaa cggttttgga tcttctgcat
    72481 atgcattacc tacgccaccc ttcattcttt gaaaatgtaa atgtgcgcct gtcgtttgta
    72541 tacctgtatt gcctgaatat gcaataatat cacctgcttt gactcgatca cctactttga
    72601 cattatattt gtctaagtgt agataccact ggtgatattc tccattgtct tcggcaatct
    72661 gtaatacctt gccgcccaat tcattattaa agatacgtgt tactttacca ttcgtcggcg
    72721 ctttaattgg tgtacctttt ggcaatgcaa aatcaatgtc ataatgctta ccatcaaatg
    72781 gtgaatgttg atatttacca aaagtctcag taatccgact ccctctaaaa aagtcttccc
    72841 attttgagtg tccttgtcct ttatgatttt gtgtcaattt atcggtatat tggttaaacc
    72901 atttttgaat gtcatctata taaataatcg taaataatag tgaaagaaaa atgataattg
    72961 ttgccaaaat acgttttggt cgctttgtca tagtggcatc catccttcat atattttggg
    73021 tattaagtat atattctaaa tcaactattt cataattaaa tgcactcata ctttagtagt
    73081 actgccaaat atagatgaag aaaatgatgg taatttgaat attttgattg atattaaatt
    73141 ttatatcgag tataataatt atacttaaga aaataacaga aacagaggag aattttcaaa
    73201 tggcaaaagt attatatatt acagcacacc cttttaatga actggtatca aattctatgg
    73261 cggctggtaa agcatttatc gaaacatatc aacaacaaca tcctgatgat gaagtgaaac
    73321 atattgattt atttgaaact tatattccag ttattgacaa agatgtatta actggttggg
    73381 gaaaaatgag taatggcgaa acgttaactg atgatgaaca aatgaaagtt tcaagattga
    73441 gtgacatttt agaagaattt ttaagtgcag ataaatatgt atttgtaacg cctatgtgga
    73501 acctttcttt cccacctgta gttaaagcat atattgatgc tatttcaatt gcaggtaaaa
    73561 cttttaaata ttctgctgaa ggaccacaag gtttactaac tgacaaaaaa gtattacaca
    73621 ttcaatcacg tggtggatat tatactgaag gacctgctgc tgattttgaa atgggtgacc
    73681 gttatttaag aacaattatg acattcttag gtgtgccatc atatgaaaca atcattattg
    73741 aaggacacaa tgctgaacct cataagactg aagaaattaa agcaactagt attaataatg
    73801 ctgaaaaatt agcaacaact ttttaaattt ttagttacac tgtgtgacgt ctcaaacgtc
    73861 atgcagtttt ttattgtatc tcttttccgc tttttataaa tatctattat tttcataaat
    73921 ttaaaatttg atattcactt taatactgat gtaaatattt ttcaaaatat attattatag
    73981 tgttaatgaa gtattttgag aaacggtggg aatgtatgaa agcacataaa attttctggt
    74041 taaacttagc agcaattatt attatttcaa ttgtagtcag tggcgatatg tttttggcaa
    74101 tgaagtggga acaaattcat ttgaaagatg gtcttaaaaa agtactaagt acgtatccta
    74161 ttaaaaactt agaaacgctt tatgaaattg atggtcacga taatccccat tatgaaaata
    74221 atgatcaaga cacatggtat atagaatcct cttattcagt tgttggatca gatgagcttt
    74281 taaaagaaga ccgtatgttg ttaaaggtag ataagaacac acataaaata actggtgaat
    74341 atgacacaac tacaaacgat aaaaaaaatg ctactgactc gacatacaaa agttatccag
    74401 taaaagtagt taataataaa atcgttttca caaaagacgt aaaagatccc gcgctgaaac
    74461 aaaaaatcga aaacaatcaa tttttgattc aaagtggtga tttaacaagc attttaaata
    74521 gcaatgattt gaaagtcaca catgatccta ccactgatta ttataattta tctggtaagt
    74581 tgtcgaacga taatccaaac gttaaacaat taaaacgtag atataatatt cctaaaaacg
    74641 catcaacaaa ggtggaatta aagggaatga gtgatttaaa aggcaataat catcaagatc
    74701 agaaacttta tttttatttt tcaagtcctg gaaaagacca aatcatttat aaagaaagcc
    74761 ttacttataa taaaataagt gaacattaat acttatgctg taattataga aacatccaaa
    74821 tcatctatta aaatcctata ttataaaaac acctcacata actcgttcaa ctgtaccaaa
    74881 ccacattaca ttagatttta ggctaactat tgtgatgtac atcaaaaacg aatttgtgag
    74941 gcgttgtata ttttacaaag gtgactagcg tttcgtatag catttccaac attactacac
    75001 tcaagcgtca cgctaaagtt cgaaatcgaa tcctttcatt caacaaaagc tcatatccac
    75061 tacaaacttc atatcaagcg tataaactat cttgtgatac tatctcgatc atatctatag
    75121 tatgcatttg tgttccgttt cactgaagta tatgtatcat cagttaagta taaaccgtca
    75181 tccttcaatg ttacttgata agcatatttc cgtgctaacc aggcaatatc tatataattt
    75241 tctcctgcgt tttcataact tcttaaatct tcaatatgtg cactaacttc agggaaaatg
    75301 attctaacaa cactttcatc aacccaatat ttgtcatgca tccatcgcac ttgatctgcc
    75361 aataaaggta actgcacatc attgaaatat agacgaaagc cgtcactatc atacatttgc
    75421 cgatatggta atggctgttt tctaatcact aacacctcgc cacccattac ggtgccttct
    75481 ctagtatcat cacttccacc cgaagcttca tacgttgttg ggtcaacctg tagtccatgt
    75541 acatctccaa tataagcatc tggtttatgt tccattgcat gtccatgtgc aatcaatgct
    75601 aatattgtag attgtgaaaa ttgaggctcc cattcaatgc gattaggatg gctactataa
    75661 attctaggtt catctatagc ctgctgaata tccatgccaa acactaatac attgattaat
    75721 gtttgcgcaa cactagcaat gatacttatg gcaccaggtg cacctactgt taatattggc
    75781 ttcccgtgat acatcacaat cgttggagcc atgttactta gtggtcgttt atatggtgca
    75841 atttcgttaa taccaccatc tactacatca aagccatcca ttgtcgtatt caataacaca
    75901 ccgtagcctg gaatcgtgat acctgaacca taaatcatac caattgatgt cgtaaatgaa
    75961 gcaatattac cttccttatc aatcacacaa aaatgagttg tttcggtatg attttcttca
    76021 acatctgtat gactaatgca atcagacaca acaccatgct ctatatcaat atttgcttta
    76081 ttgctatcaa tgagcgtact gcgtgctttt aaataatcat catcaattaa tgactgtaca
    76141 ggcacctcat gaaaattatc atccgccaag tattgcgcac gatcactata tgctaaatgc
    76201 atcgcttgta tcaaatgatg caagtaatca acagatcttg gacccataga tggtaaatcg
    76261 acatgttcta ataacttcaa tatttgaatt accgtgatac cgccagaact agatggtccc
    76321 attgaataaa tgtcatagtc tttaaatgtt gcactgattg gcgctttaat ctgaatgtca
    76381 tatttggcta gatcctctaa agtgattgtc ccaccacatg ctttgacaac attgactaat
    76441 tgtttcgcaa tgtcaccttt ataaaatgca ttaaaccctt gttctcttaa tatttgaaat
    76501 gtcttaccta attcgggttg tacaatccaa tcaccttcac gccaatattg attttcatgc
    76561 gtaaatactt gtgccgtttc atgatacttt gtcaatcgtg cgtgttgctg gcgcgaatat
    76621 ttttcagtag cccaattggc tgcatgacct tcaatggcta gttcaattgc aggattaatt
    76681 aaatcttcca atgacaattt agcataacgc ttgtgaatat aatcaaacag ctttggaatt
    76741 gctggcacag cgacagtttt accatgtgta gtcatatcaa aaaatgattt atattcgcct
    76801 gaatcatcta gataaaattg tttgtctaca tgttcaggtg ctgtctcacg tgcatcaaac
    76861 gcagttatac tgccagtact ttgctcataa tatagcaaat acccgccacc accaatacct
    76921 gatgcaaatg gttctaacac attcaatgcc agttgaattg caatcactgc atccatggcg
    76981 ttgccacctt gatctaatac atccttacca attttagccg caagaggatg tgatacggaa
    77041 attaaccctt ctttagatgt ttttgtctgt ttgtcattta agttaatgac catactatat
    77101 cctcctactt tctgttaaat atttaaaaca ttattgatta atggcttttt ctactttttc
    77161 taaatcttga cgttgctcgt taccagtatc gacaagtggt gtaatcggtg atgcaatttt
    77221 aaatttatcg ccacgataaa acttaataaa ttgatcctga tctatcgcat taactactgc
    77281 ttgtctcaag tttggatgcg tcttaaatat acctttttta atatttagca ttaaaaagac
    77341 tgacttgcgt ccatttttgc gaataatgct taaattttta tccgacttaa ttaaatcaaa
    77401 atgtttttga ttcacatctg ccaacatatc aattgaatga tttctaagtt ctgacaatgc
    77461 attattcggg tcaccattaa acttcaatgt aatattttta attttagctg gtccataact
    77521 acctttttct gtttcgttga atcctggatt acgttgaaac gttgcttgat atgcattttt
    77581 ctgtgtcata atgtatgcgc cacttgcata cagcgcattt ttcccatctg aatttgcagg
    77641 aattgtactg ctatccccat atccttttgg atattcttga tttacttgat taacaaattt
    77701 tttagataaa atgcctgccg aagagtgtgt taagtaattt acctctcgag gcatcgattg
    77761 atctgtcgta attttaacaa tttgataaat accgtcttta ttatttactt tttgaccatc
    77821 tgtcgttaac gatttgacgt tataagcttt aatcaactta tcatagattg atttatcgtc
    77881 cttgtctttc tctttacgca actgatcgat gtcctcatct tttaatatct tgatgtcatt
    77941 tatatgtttg tgcatattgt aagtattatt gttaggcaca gactttttat cacgtgctct
    78001 atctaaagaa aacttaacat cttcagccga tacacgctct ccagtattac gtgcttgtcc
    78061 attgaccact ttcgcaaaat aatcatcatc tcttaacaag aaataaaatg ctttattgtc
    78121 cttattcaca gcataatcat gacttaacga acctttcgtt gttaaatgat cattttcatc
    78181 taataataat aaccttgtgt acatattcat attaattgaa tatactgacg gcgcaattga
    78241 acgtattgga tccaatgtag gaatttcacc atcttgttgt gtcatcacaa gtggccgcgt
    78301 atctcgttct ctactattgt tgtaatcaaa ttgttgccat attaatgcac gtgaatttgg
    78361 caatccaaca ctatttttat ctaacacttt attgtcatat actaaattct tttttgatcc
    78421 atataaaggc gccatatacc ctttatcaaa tacaacttca tcttcaattt gcttatatgt
    78481 ttgtttaaca tctgcttcat tttgagtaga agctttattt aacaactggt ctacatgttt
    78541 atctttcaat aaactatttg atcctgtaga actaaataat gccgtcatag catagttcgg
    78601 gtcaccaaac actgtcatcc agtcatcaat ttggatatca taattgccgg cttgacgttg
    78661 tgtacgatag ctaccataat ctggttggat attcatcttc acgttaaatc ctgcattttc
    78721 caattgatct ttaacgatat tcatatcatt ttcataactt gcttgtccta ggaaatgtat
    78781 tgttggtcgc tcgcctttca cttcaacttt cgatgacttt tgagccactt ctgatttcgt
    78841 agggacacca caaccactta ataccaacgc taaaactata attgcgatac taatgatttt
    78901 cttcacatct atccctacct ttttaatgaa ttcttggatc tagtgcatca cgcactgcat
    78961 cacctataaa attaaatgct aaaacgacga acataataca aacaccaggt acaatagcta
    79021 aattactgtg cgtttccaag tagttactac cggtacgtaa aatgttgccc cattcagcta
    79081 catcaggtgc aacaccaagt cctaggaaac ttaaactact tgttgttaat acaaccacac
    79141 ctatatttaa tgaaaaacgt acaatcatag gcgcaatcgc attcggtaaa atataacgcc
    79201 atatgatatt ccaagtgttt tcaccagtga tacgtgctgc atctacatat tccatgcgtt
    79261 taatttctaa aacactggca cgcattgtcc gtgcaaatga tggtatatta ccgatactta
    79321 aagcaataat taaatttgga atacttgctc caaatgatgc aataattgcc accgctaaca
    79381 ataatgatgg aattgcaaac actacatcta aaattcgcat tattaaatta tcaatatgat
    79441 taaaataacc tgcgatagtg cctagtaaca caccaaaaat aactgcaata actactgaaa
    79501 taattgaaat tgaaaatgtc agcttcgttc ctacaactac gcgtgtaaat aagtctctac
    79561 cgaaatcatc agtaccaaac ggataggcta gactcggtcc atgtaacagt gcattgaact
    79621 gatttttagt agccaatgtc gtatcaaatg taaattgtga cacaattgat aatgtcagca
    79681 tgtagactaa aataagtaac ccgataatcg caatacgatg tctagtagtt tttcgtataa
    79741 acgattccca cccgttataa ctatgtattt gcgatgtacg ttggtaacgt ctaatactta
    79801 caaacattaa taatgtaaat acgttgcctg ttaatgtcat caacaataac aacacttcga
    79861 cgatacgtcg ccataggtca tgatgcttcc atgtttgttc cgttgttaaa ataataatta
    79921 aaatgatggt taaaacgatt agcaatgttt cagcaatata gaacgtatcg gccacataac
    79981 ctttaaaaag atttaatgca ctcgttaata taactaaaat ataagttgct atggcgtaac
    80041 ttgcgaataa ttttaaggaa gctatctttg aattaagttg tgccatatgc ctcacttcct
    80101 ttcgttgatt tcactacgta attttggatc gattaaagca taaaatatat caataattaa
    80161 gtttgctaaa gatattacaa ttgatatata tacgacccca cccatgactg ctggaatatc
    80221 aggtattagt tgtttttgga cgatataacg cccgatacca ttaatgttaa atacttgttc
    80281 cgtcactgct gaaccgccta gtaactctgc cactagaaga ccaactaacg ttacaattgg
    80341 aataatggca tttttcaaaa tatgtttaat aacaacttgt gtcgtcgata atccttttgc
    80401 ataagcagtt aaaacataat cgctgcgcat tacttcaagt acagaagacc ttgtcatacg
    80461 cgtgatagaa gcagcaatac ttgttccaat gacaagtaca ggtaaaatca acgatattgg
    80521 atgttctggc atataagatg gtggcaaaat atccaatttc aatgagaacg ctaaaatgaa
    80581 taatagccct tgccagaaac ttggaataga taaaccaatt aatgcaatta tcattaacgt
    80641 gatatcaagc caactatttc gcttcatcgc actgataata ccaattggta ttgcaataat
    80701 taatgccacc attagcgcta atactgcgac aattattgta attggaattc tttcgccaac
    80761 tgctttagtc acaacctcat tccctttgta agtcgtacct aagtcaaagg taaaaacacc
    80821 cttgatggta tcccacaatt gaataaaata aggttcgtta agatgatgta atacattgaa
    80881 ttgatgtatc tgtgcctttg ttgcattttg tcccagtatg ctataagccg catcaagcgg
    80941 tgaaaaatac agaatggtaa acacactgac aataacacca atgatgacaa tcacagccat
    81001 gacaattcgt tcaaaaatat atctaactaa tggctgtaaa taaaaagtca ataagatgaa
    81061 catcggcaag gccaatatca ctttgatcat gatgaactta tgaaataata cattttcaaa
    81121 gtatgttgaa aaatgtgctt gttcaatatt ctttgaactc gtattagaac tttgtgcctt
    81181 gaatattttt aatgcttctt tatgtatttg tgtggatgac ttttgctgcg ataaatattt
    81241 atatttttga tgtaacgcct gttcaatttc tgaaatttca gaattattag cgtaaaaatt
    81301 tttcctctta gcagaaaaga aaaactttat cactgcatat aaaaatattg gcaagcttaa
    81361 taccgataat acaaacttgt aaaatgcctt gtgatacaac gacttaaaag attctaaata
    81421 agtaattatg ataatcactc cttgtaaaat aatcttgagt agattactat gatatacaaa
    81481 agtatagaat aaatttacac atttgtgaat agggaggcac aacatcatgt caaatttatt
    81541 agaagtcaac agtctgaatg tacaattcaa ttatgatgaa actacagttc aagcggtaaa
    81601 aaacgtctct ttcgaattac gaaaaaaaca tatcctaggt attgttggtg aatcaggatc
    81661 aggaaaaagt attaccgcta aatctatttt agggctacta ccagattatc cagatcacac
    81721 attaacagga gaaattattt ttaatgggca atcgttaaat aatttatcaa cttcagcgtt
    81781 acaacaaatt cgaggtaagg atatttcaat gatttttcaa gatccactct cttcgttgaa
    81841 tccaagatta acgattggca aacaaattac agaagtaata tttcaacata aacgtgtatc
    81901 taaatctgaa gcaaagtcga tgacaataga cattttagaa aaagtaggta taaaacatgc
    81961 aactcgacaa tttgatgctt atccacatga actttctggt ggtatgcgtc aacgtgtcat
    82021 gatagcaatg gcattgattt taaagccaca aattttaatc gcagatgaac caacaacggc
    82081 attagatgcc agtacacaaa atcaattact gcagttaatg aagtcccttt atgagtacac
    82141 agaaacatct attattttta tcactcacga tttaggcgct gtgtatcaat tttgcgacga
    82201 tgtgattgta atgaaagatg gaagtgtcgt tgaaagtggc acggttgaaa gtatttttaa
    82261 atcgccacaa catacctata caaaacgctt aatagatgcg attcctgata ttcatcaaac
    82321 gcgtccgcca agaccgttaa acaatgatat tttattaaaa ttcgatcgcg tgagcgtgga
    82381 ttacacatca ccgagtggca gcctataccg agcagttaat gatattaact tggctattag
    82441 aaaaggcgaa acattaggca ttgtcggtga atcagggtca gggaaatcga cattagctaa
    82501 gacggtcgtc ggtctaaagg aagtgtcaga aggctttatt tggtataacg aattaccatt
    82561 aagtttattt aaagatgatg aattgaaatc tttacgacaa gagatacaaa tgatttttca
    82621 agatccattc gcatctatta atccaagatt taaagtcatt gatgtgatta aacgaccact
    82681 aatcattcat gggaaagtca aagataatga tgacattatt aaaactgtcg tatcgttgtt
    82741 agaaaaggtt ggcctagatc aaactttctt atatcgctat ccacacgaat tatctggtgg
    82801 gcaacgtcag cgtgtaagta tcgcgagagc acttgctgtt gaacctaaag tgattgtttg
    82861 cgacgaggca gtgtccgctt tagacgtttc aattcaaaaa gatatcatcg agttattaaa
    82921 acaattacag ttagacttcg gcatcactta tttattcatc acacatgaca tgggtgttat
    82981 caatgaaata tgtgatcgcg ttgcagttat gaaaaatggc gaaatcgttg aactgaataa
    83041 cacagaagat attatcaaac atccgcagtc agactatgca aagcaactta tttcagaagt
    83101 agcagttatt gctaaataaa agtcatgcgt tgtgcaactt tatcactgta tggtctgaaa
    83161 taaattgcgc gacttctgat gtgttatgtt agatgacttt gtcatactaa ctatattgtg
    83221 ctaagttagg gctcggcacc atatcaagtt ttaggtgctt tgccatgatt taagagtcac
    83281 ccccatactt tgggcatttt aacgccagaa taaatccccc gccactatgt gaagtgcggg
    83341 ggattattta tattttatta gaatattcag atttttgagt gtgtcaactt agcttagtca
    83401 atgtatattt aacgtcactt actctttttc tttcataatt aacacattca aataaacttt
    83461 gatcaaaaaa cacaaagtta aaagtaccat cttgtaatat gctctcatac attatcccgt
    83521 catatttaag gcttcgaata taatcagcta aatattgaaa tggcaaataa tctattcctt
    83581 gttcatcgct tggatttgtt attcctttat gaatcttttt taatgtttgg taatttacaa
    83641 aatactttct aaatccatca tcgccagctt tgattgcatt actagttaaa ttagttaaat
    83701 tcgcaatttt caatttctct tttgtcacgt ttttttgtaa cttaacctta cctatataaa
    83761 taatgtcatt atgcttaggt ttaacttctt ctatactgac ctgttctttt gtactaaggt
    83821 ataatacgct tatccattta gaattcaatc ttcctgccgt tgcaaatccc tttggtggtg
    83881 acattagttc acttttctct gtaatgaact taactattct agatctatat aatggttcaa
    83941 atctttctct aaattcctca atactatagt aattagtagt gatatcgaga aagaacgcta
    84001 aattctctaa attgatcata ttattatgaa atctattttt atacttcaag ctctcacaaa
    84061 atccatccca gtcattattt gctacaatta gatttttatt tgtatatttt ttatcgttta
    84121 tgattttagc gcctactaaa tcttccaaca ctcgtctatc taaattttca tcatctttaa
    84181 aaagttcatt taaaatacaa cttatttgag cttcctcaac attaaatata ctccagtcgt
    84241 cttttaatgc tatttcaatc tttttacctt cttttgggct aaaagtatct ggtaaattta
    84301 tactaatatc atataattct aatgctggtc ttaaataatc tctaataagt tctaatttat
    84361 ctatgtcctt agtcgtatca aatattttaa caccaagatg attgttatca atatcacaat
    84421 tgtcaaattt gctattatca tttgcaatga tttctacgat ttcagtatta ttaaaacatt
    84481 tttcacatat tttcattttg agactccaag tatctattca taatttctag gtgatgcatg
    84541 atagataacc ttttaattaa acctaatcct ggatacttat tattttcatt taattcttca
    84601 aattgtccaa gcgcataaga tctattttta atatctaagt tttttgacca tgttactaat
    84661 ttatctacag cctcaaaaaa ctttttacct ggatttgatc tatcattatt agaatcagag
    84721 acaaaatgct taactcttag ctcattagcc tcatcaaaat agacaatatg tatcgcaata
    84781 gctaatggcg aaaagccacc gtctacatat tctccaccta taacagaata gtctgaaaat
    84841 gctacgtatc catcttcctt ataataaagg tgatcgtcgc tataaaactc gtcatcatta
    84901 tctaaataat caacattacg acttagttta ttaaaaggat ccataatgat gcctttgttt
    84961 ggaatattac gcaatttacg tttaatgtgt ctatcatctg gtacaaaaac catttcaagt
    85021 ttatttttat ctccaaacat ttcccaatca tctacacttg ttaaaatagc taaactatta
    85081 cttgaaaatt gctgtttcaa cctatcattg ccttcatcag aaggacccaa gtaagctttt
    85141 tgaataacac ttttgttttt tgttattatt tcagttaaat gtgcaacagt ttcgttacta
    85201 atctcttctt cagttaactt tgaatttact actaaattta tagagaatgc tttttcacca
    85261 cagtattgca aagtattttt aaatgtggtt gtatatttaa ttggttcaat tataggttga
    85321 atacaatcac caatcaaacc tttctctaac aattctctaa ttgcaaatag ttcattttgt
    85381 ttcccacgca aataaggata atacatatta atcctcctta agaaaatgat atagtttatt
    85441 tttatctttc tttgtcattt ttgcgaaata taacaagaac ttaatttcag ccggcgctgc
    85501 taaaaattca tctttgtatt ctttcatgtt tcctcttttt ttcaactcag aaatcatatt
    85561 gttatgcagt ttcgtcattg gtatttcttt aaacacgtca aataacacat catactctgt
    85621 gaaatcatta taagttggca catccatatg attttgtgca ataactcttt ttctttcttc
    85681 ttttcgtaaa aagttgtaca tcgcttttgt gtttaagaga ctattgtttt ctttaggttt
    85741 ttgaacttca ctcagtgtat ttctagttgt taacactaaa attccaactg ttgtatcttt
    85801 gtatctagcc ataactttat tcagatgttt gtcatttgta ataactacga cataattaaa
    85861 cacttcataa taatcattaa tttgattatc taatctatcc agcttatcta attctgtttt
    85921 aatctcatag acaacgcctt tgccgtttaa caatataaaa tcagcaatac ttttccctat
    85981 gggcatctca gaaagtgcag tagttgtatt aatagaatgt cgtcctagaa ggagtttatt
    86041 aagtattgtg tttttgtaaa aatattcgtt acgataagac tttttaagat agttatataa
    86101 agatttcatc ttctgtatta cattgatatt tttaggtccc tgtatataaa aatcatatgc
    86161 ctcatcaaca acatcacttt tatttttgat tgcaatttga tgtaatatct tttgactaaa
    86221 aacacgatta atcatatttc tattatccat agctacgcac ctccttcaac atacatttaa
    86281 tacttatcct gttaatatta taaactattt taaatgccta tagacattat ttctatacac
    86341 atacgattat aaaattgtat tgaaaccaaa tgatggttgc caaacacatg gtaactaaca
    86401 aaagatagaa aataataatg ccccctacac taatctagcg agggggctga atcattacac
    86461 accgtatttt tcagttgttt ctgccacgaa agattcaatg gcttttcttg ctttacgctt
    86521 ttctttcaat ggcaaatcac caatcatttt tttaaggtga tgtgggttta caacaccact
    86581 atactggtag tcatttgaaa ttgtttttag ggcttgttca tcgatagatc tctctccagc
    86641 aaatcctttg aactccgctt cttttttaat actttcgaaa ttaacatatt cttgatcgat
    86701 atcatcatcc ttatttaaag aaggtacaac attgtcgatg aattctctaa ttagatctcg
    86761 ttttaacctc aatgtcggat catctgcatg atctaaaatg cgtctaattt gttcttggtt
    86821 acgacgttgt tccgctttgt cttcaagatc aatttgtctc aatatattca taatataatt
    86881 cacattaatc gtatcattac gcatcatttc tatttcgaaa tcaatatcat ttaaaatgga
    86941 tactttattt ttctcagccg tcgctctttt tacttgatcg tacacagcta aatatttact
    87001 tttatagtct tcattctctt gttcatccat tccaatttca tcaattgtaa actcaaactc
    87061 gtcaaatgct tttaaacgta atattatttt agctaataaa cgataagctt caacaaagcg
    87121 ctttagctct tcttcatctt gaatgtcatc aaccatgtgt ggtgtcggca caatcatttt
    87181 aagctcacga taagcgtcca taaattcttt tttatactct tcataactgc gcattaaaat
    87241 tgtatccgta tcatttgttt gtgagaatac tctcagtgca tcgtctgtct cttttttcaa
    87301 gtcacgatag tttacaattt taccaaatgg ctttgattct ttttcaaccc tatttgtacg
    87361 tgaatacgct tgaattaaat catgatacat taaattctta tcaacatata aagtgttcag
    87421 tactttacta tcaaaaccag ttaagaacat attaacaacg attaagatat caattttact
    87481 atctttaacg ccctttttaa cgttttttga aatatgatta aaatactcat tagttgtgtc
    87541 tgttgaaaaa ttcgtctcga actttttatt ataatcacta atcattatct ctaatttttc
    87601 acgtgaatga tatggcactt caccatcacg atcatcttca ttaggtttaa acgtaaatat
    87661 accagctatc gttaacggtt gttccaactt tttgttaagt cgcttaaatg tctcataata
    87721 tttaataagc gcgtgaatac tttggactgt aaatatactt gaatattgac gattacgtgt
    87781 atatttatca tgattattga tgatatgtcg tgttactaat tccacacgtt tatccgctaa
    87841 ccatacttct tccgtatcaa ttgcttcaac catgctgtta tcttctgctt ttaaagcttt
    87901 atttttaaaa gtattaatat agtcaactga gaaaccaagt acattaccat catgaatggc
    87961 atctctaatt aaatacgtat gtaagcatct accgaaaata tctgcagttg ttctaccatc
    88021 ttgactacta ttttctggaa aacgtggcgt accagtgaat ccaaagtatt gggcattttt
    88081 gaaatgttgt ttaactagac gatgcatgtc accaaattga ctgcgatgac actcatcaat
    88141 aataaataca actttattcg ttttatactg ttctaataaa ggggcattcc cttgaatcgc
    88201 tttagccatt ttttgaatcg tcgttacaat aagtggcaaa cttttatcat ttagttggcg
    88261 taccagttgc gaggtattaa aagttttgtc tacagcaccc ttagcaaatt tattaaattc
    88321 ctcttctgtt tgactatcca agtctttacg gtcaaccaaa aagataactt tcttaatgtc
    88381 atcttgctgt gataaaatct gactcgcttt aaaagaagtc aacgtcttac cacttccagt
    88441 tgtatgccat acatatccat tattccctgt ctcagtcgct tgttgaataa gtgcttctac
    88501 cgcatacact tgatacggac gcattgccat cagtattcta tctgtttcat taataatcat
    88561 atagcgcgat atcatcttag ctaattgaca aggtctcata aatgactcag caaacgattg
    88621 caatgtattg atacggttat tctgtttatc actccaataa aacatgtgac tcttcaatag
    88681 ttcgctatca ttattagaaa agtatcgcgt ttcaacacca ttactaatga taaacatttg
    88741 tatgtagcgg aataagcctg tgtaattttg tttgcggtaa cgttttactt ggttaaacgc
    88801 ctcattaata tcaatacctc gacgtttcaa ttcaacttgg acaaggggta gtccgttgat
    88861 taatatcgtt acatcataac gtgctttata tgtatcctcg acagatactt gattcgtcac
    88921 ttgaaactta tttttacacc aacttttcgt atctaaaaac gacaaataaa tctcagactc
    88981 atcatcacgt ctaagtggta atttatcacg taaaatacgg gcactctcga aaatactttt
    89041 tccatcaatc atcgttaaca gacgttgaaa ttctttatct gttaagggat tgccttctaa
    89101 tttgtccgca tgacgctcat ttaaaatcgt tctaaaatta tcaagcaatt gcttattatc
    89161 acgtatcgtt actctttcgt aacccaattg ttcaagttga ttcatcattt cattttctaa
    89221 tgcgtattca ctttggtatg ccattcatat ccccttccat acactttcta ttgctctaaa
    89281 tatatcataa actttaatga aaaatgtttg ttttttatct tcaaacgtaa atttattcta
    89341 attttattgt cttatctttt aatatttgtc tttgaggtaa gtcgtatact aaaatttgaa
    89401 tacaaataat caaatcattg ataaattttt tgtctacgat taatggaggg acttgaatgg
    89461 tgttaattac ctatcaaatc attttatttt ttattattag tctaagttac tatttaactt
    89521 taaatcatta catggcagtc actgtaggta acttcacttc aatattcggc atgttcgcag
    89581 ccatactctt tatgtactac tacctactct ataaaagtcc cgaatacaat caacgcaaac
    89641 gatttaaaca tttcattcat atcactaatt tgataataat tgcttttagc accttcgtat
    89701 tagttcattt agcattaaaa ttattcttca gcatttaatt tccatctatg aaaaaagcaa
    89761 agctcaaatc tgaactttgc tttaatttgt cacgccttta tcattttcaa aatagcctct
    89821 atgccagttt tacaaacttg tagcaacaat ttttcatcaa gcaactgaat cacatcaaaa
    89881 acttcaattg tttacgttgc aactaacttt atgttagaac gcacacaata tctaatgttt
    89941 aaaatctatc gtcgcaagat gcttcctgta attatcaagt gccattttcg attgggttat
    90001 acaatctaga atcgcatgat aatttaatgc tacaaatcga tagtacaata tatctaccgt
    90061 gaataactgt gcaaatagtg acgttgtagc cgccatacgc atttcatttt catcagttct
    90121 gccataaatc aatgcatagt ctgcaatttg agccactgga ttattagctg tactagatat
    90181 agttatgatg ggaatactgt aatgtgtggc cacctgtgca attgactgca attcactatg
    90241 actaccttga ttcgtcacaa aaatcatgca atctctatca tcatgcgtcg caaatgttga
    90301 cacaagtaaa tgcgtttcat gtaataacct gacatttaag ccaatacgag ataacttttg
    90361 aaaaagatca ccaatagtca aactcgatgc gccaaatcca aataaaaata ttgtcctggc
    90421 atttttcaac acatcacaaa ttgcatcaat ttgcgcatcc ataatattag tagctacaaa
    90481 tcgcatcgta ttcgttgctc tagcaatcat tttatttttc aaagtttcta cagattcatt
    90541 ttcaatcaat tctaaatgtg gattggttgc aatatcttcg ggtaagtatc gagatatcgc
    90601 aatctttagc tcttgaaaac cttgatgtgt cattttccga ctaaatctaa caattgatgc
    90661 tgtactaaca ttcgtaacat ctgccaaatc attcacagtc atatcaatga ttttatgtgg
    90721 attctttaaa atgtaatcag cgattatctt ttctgtcttc gtaaaatcac tcaactgctt
    90781 atcaatgcga tataaaatat ttgtcatcat taatcaccca acaaatctgt ctgtcgcatc
    90841 gcctttgtcg ttccaaataa atatgtacaa acgaatccac cagcatacgc agcaagtaat
    90901 cctgcaatat aacctaaata catattatct gagattaatg gtaatagtga cacaccactt
    90961 gggcctattg ctttggcacc aatatgtcca attccaccta ttacagcgcc accaatacca
    91021 ccaccaatac aagcagttaa gaaaggtcga cctaatggca aagtcacacc atagattaat
    91081 ggttctccga tacctaggaa accaactggc aatgcacctt ttaaagtatt acgtaatgtt
    91141 gtgttgcgtt tacatcttac ccaaagtgct aatgcggcac ctacttgtcc agcaccagcc
    91201 atcgctgcaa ttggcaataa gtaagtagca cctgattggt taatcatttc tatatgaatt
    91261 ggcgtaaaaa tatgatgaag ccctaacata actaacggta ggaagcttgc accaatgata
    91321 aatccactaa atacgccacc aatactaata attccgttaa ctactgaaac taaactgtct
    91381 gaaacaaaac ctgctaatgg cataaagata aagatagtta atagtcctac aatcaacaat
    91441 gcaatagtcg gcgttacaat aatatcaatc gcatttggca caattttatg taatctcttt
    91501 tcgacaatac ttaaaatcca aacggcaaaa ataacgccaa taatcccacc ttgtccaggt
    91561 tgcaatggtt ctccagtgaa gacattcatt aaaatatttt taccagcaat acccgttaat
    91621 aacgttgtac caccaatcac gccaccaagt cctggtgtcg caccaaattc tttagccgca
    91681 ttaataccag tgaaaatagc taagtatgct aacataccgt ctttaatgac attaaatact
    91741 gttataagtt gcgtaatcca agcacctgaa atatagcctg ccaccattaa gttactcagt
    91801 actgctgcaa taccaccaat taatccagct ccaataaatg caggaatcaa cggtataaag
    91861 atattggcaa ttgatttcaa tactttattc aacttaccat tcttttgttt tgctttatgc
    91921 gcttccttat tcgcctttgc tttatcagct gcatatgatt tatagtccat tttttcacta
    91981 tcattgtgat ggtgtggtat tgggtcacct agtttaacac cacttaattc cgccatatga
    92041 ttagccactt tattgactgt accaggtcca accacaactt gaatgcgttc atcgtgtata
    92101 acacccatga caccatcaat atgccttagt tcttggtcat ctactttatt ctcatctaat
    92161 actttaatac gcacacgtgt catacagttc atgacactat ctatattatc cataccacct
    92221 actgcagcaa taattcgttc tgcaagttgt tgttctttgg tcatttaaat ccctcctaag
    92281 gttgtctatc tctgattgct cgtttaacaa tgtcaccatt gtttaataac cgtcttgttg
    92341 cttcttcctt agaaatgcca cacataccca taactgtcgc aactttcaca tcatgctcag
    92401 atacctgata taacgccatt gcttcatcat atgtgatagc acatatttct tgaataatac
    92461 gcactgaacg gtcgatcagt ttttgattgg ttgctttaac atcaatcatg aggttatcgt
    92521 aaacttttcc gacaccaacc attgtgatgg ttgaaatcat atttaaaatt aacttttgtg
    92581 ctgtaccaga ctttaaacgt gttgaaccag ttaatacttc tggaccaact ttaacttcta
    92641 ctggatactg cgcaatttca cttataactg catgttcatt gcatgaaata gatactgttg
    92701 tagcaccgat tgtgttagca aatgttaaac cgcctataac atatggcgtt ttgccactcg
    92761 cggcaattcc tataacgaca tcttttgatg ttaaatctat atttttcaaa tcttcttccg
    92821 ctaatttttt gtgatcttcc gcaccttcta cagccatcgt catagcatgt tgtccaccag
    92881 caataatacc tataatttca tgagggtcag tattgaatgt aggtacacac tccgctgcat
    92941 ctaagacacc caaccttcca cttgtacctg caccgatata aatcaatcgt ccaccctttt
    93001 tatactgtgc aattgttttt ttaattactt ttgtcaattg tggtattgcc tttcgaactg
    93061 ctaacgggac ttgctgatct tctttattca tcgtaattaa agcctcttcc acagtcattt
    93121 catcaagatg catcgtcgct tcattacgcg cttcggtcgt actattttcc atcacttctt
    93181 acactcccta gttttttgaa aatcaaatgt atcattcggc tcgatacaac ttaacagtgg
    93241 taagtcttct ttaataattt gtgcaacaac attcacattg tcatgtgcac taagcgtttg
    93301 tctcacaatt tgcatttcgc cttgataacg tccgttattc aaattatcaa cggttactga
    93361 accaatgcgt cgttgcgtcg taaactgtgg ttgaatcgaa tgtggacata tttgtcttga
    93421 cgtttccgaa cgaatgacat tttccggatt atccgggcgt actttatgac acatatcgaa
    93481 aaggtaagtc actgtcgtat caaacacttc ttcaatacat aacgtgaaat gcctatgctt
    93541 gcaaaaatct ataagttgtt ttgcctgcct catttcaatc aatgagtctc caactaacac
    93601 ttcagataca ccagtttctt gtaataattt agctgcaacg acaggatgac tatgtctcgt
    93661 tgcttcaatt gttggcaagc ctttatgcaa aggacctcgc aaatcactcc ctacaataaa
    93721 accatatatt tgtgcctttg gattaaattg ataaatgagt tcatttttct tattgaccaa
    93781 gtcaacagat aatcccgtat ctggtcttgg ataatagtta tgacaaaatg aaagtaatgt
    93841 aaaatcattc aattgttgat gtaagcttgt taacaattcc cgggaaataa tacttgcatt
    93901 caaacagcac tttaaaccct gtgccattat cgcttcgatt gcctcaattg atgtactatg
    93961 atcgatacga atcataaatt gtgcatcata ttgtcgaaga tggtcataaa aagatggtgt
    94021 taaaatagat ggattagcat ctatgaggta agtcacttgt tcatgtttta ataaattgag
    94081 tagttttgtg aaataatgat attttgtctc gtcatcttct tctggtattt gtacagatgt
    94141 aaaaatcatt tggtaacctt gtttaatcat tcgcttaata tacgcttcat ctaaaggttg
    94201 tcctaaatac actgaaaagc ctgtcaaagt agccctcctt aacaatataa ttattaggaa
    94261 aatatagttg agttgtgtaa tcgcttacat tttactataa gagaaaacac attacaatat
    94321 taatcagtta aagcctgttc attgtaataa tcttacatat ttctgtcaca agttaattat
    94381 tacaccatca aagattatcc tttcttttaa gtgctgataa tagctgctac tgctggatta
    94441 ttacaataac ttttatacat tttattcagg attatcttat attatgtttt aataataatc
    94501 tgtgaacaat taagagattt gaaattgaat ttaataattg tattgaaaac gcatacttca
    94561 ccatgctaaa ataggagtcg caaacaaata agattcaata agatgtgatg gtaagctaga
    94621 taaactaacg tatcagtttt atatactact tagtcaattg cttcttttac attaattaca
    94681 tacaccaacg tgttactaag taagattagg catgagttaa attaagctgt gatggttacc
    94741 aacacagtct atttgctcgt gtcttttttt attgaatctt aaataataaa tacaactttg
    94801 gaggttggac aagtgaggaa gaaacttttc ggtcaattgc aacgtattgg taaagcgcta
    94861 atgttacctg ttgcgatttt accagcagct ggtctgttat tagctatcgg tacagctatg
    94921 caaggtgaat cattacaaca ctacttgccg tttatacaaa atggtggcgt acaaactgtc
    94981 gctaaattaa tgacaggtgc tggtggtatc atttttgata acttgcctat gattttcgca
    95041 ttaggtgtcg caatcggatt agctggcggt gatggcgtag cagctatcgc agcattcgtc
    95101 ggttacataa tcatgaacaa aacaatgggc gactttttac aagttacacc taagaatatt
    95161 ggtgatccag cgagtggtta cgctagcatt ttaggtatcc caacattaca aacaggtgtg
    95221 ttcggcggta ttataatcgg ggccctggca gcttggtgtt ataacaagtt ctataacatt
    95281 aacttaccat cttatttagg tttcttcgct ggtaagcgtt tcgtacctat tatgatggct
    95341 acaacatcat ttattttagc attcccaatg gcattaattt ggccaacgat tcaatcagga
    95401 ttaaatgcat tcagtacagg attattagat tcaaatactg gtgttgccgt attcttattt
    95461 ggtttcatca agcgtttatt aattccattc ggtctacatc acattttcca cgcaccgttc
    95521 tggttcgagt ttggttcatg gaaaaatgca gctggtgaaa ttattcacgg tgaccaacgt
    95581 atctttatcg aacaaattcg tgaaggcgca catttgacag ctggtaaatt catgcaaggt
    95641 gaattccctg ttatgatgtt cggtttacct gcagcagctt tagcaattta tcacacagct
    95701 aaacctgaaa ataagaaagt agtagcaggt ttaatgggtt ctgctgcttt aacatcattc
    95761 ttaactggta ttacagaacc attagaattc tcattcttat ttgtagcacc attattattc
    95821 tttattcacg cagtacttga tggtttatca ttcttaacat tgtacttatt agatcttcat
    95881 ctaggttata cattctcagg tggtttcatc gactacttct tactcggtat actacctaat
    95941 aagacacaat ggtggttagt cattcctgta ggtcttgtat acgcagttat ttactacttc
    96001 gtattccgat tcttaattgt aaaattaaaa tacaaaacac caggtcgtga agataaacaa
    96061 tcacaagcgg ctactgcttc agcaactgaa ttaccatatg cagtattaga agctatgggt
    96121 ggcaaagcaa acattaaaca tttagacgct tgtatcacac gtctacgtgt tgaagttaac
    96181 gacaaatcta aagttgatgt tcctggtttg aaagatttag gcgcatctgg tgtattagaa
    96241 gtcggcaata atatgcaagc aatttttggt cctaaatctg accaaatcaa acatgaaatg
    96301 caacagatta tgaatggtca agtagtagaa aatcctacta ctatggaaga cgataaagac
    96361 gaaactgttg ttgttgcaga agataaatct gcaacaagcg aattgagcca tatcgtgcat
    96421 gcaccattaa ctggtgaagt aacaccatta tcagaagtgc ctgatcaagt gttcagcgaa
    96481 aaaatgatgg gtgacggtat cgctatcaaa ccttcacaag gtgaagttcg tgcaccattc
    96541 aacggtaaag tacaaatgat tttcccaaca aaacatgcaa ttggtcttgt atcagatagt
    96601 ggtttagaac tattaatcca catcggttta gacactgtta aattaaacgg agaaggcttt
    96661 actttacatg ttgaggaagg tcaagaagtt aaacaaggtg atttattaat caactttgat
    96721 ttagactaca tccgcaatca tgcaaagagt gatattacgc ctattatcgt gacacaagga
    96781 aacattacaa accttgattt taaacaaggt gaacatggca acatttcatt tggcgatcaa
    96841 ttatttgaag ctaaataatg cttactataa acaggtgcgt ataccttcat aaggtgacgc
    96901 gcctgttttt tctttgctat tgtattttgc agcatcattg atagttcgct ctccccttaa
    96961 attttgaatt ttaagatcat caattaaagc cccccttcat actcatttcc taaaaaatat
    97021 taattgttca ctattgttag cgttttcaca acaaagtcaa cttccttgac cttacactat
    97081 attcgaggct atcattttaa gtgtaaatat agagaaaagg tggctttttt tatgaaacaa
    97141 cgcattggag cttacttaat tgacgctatt catcgagcag gcgtcgataa aatttttggt
    97201 gttcctggtg attttaatct cgcttttcta gacgatatta tcagcaatcc caatgtagat
    97261 tgggtaggaa atacaaatga attaaacgca agttacgcag cggacggtta tgcccgtctt
    97321 aatggactcg ctgcattagt tactacattt ggtgttggcg aattaagtgc cgtcaacggt
    97381 atcgcaggtt catatgctga acgcatacct gtcattgcga ttacaggtgc gccgacacgt
    97441 gctgttgaac aaggcggtaa atatgtacat cactcacttg gtgaaggtac atttgacgac
    97501 tatcgaaaaa tgtttgcaca tataaccgtt gcacaaggtt atatcacacc tgaaaatgca
    97561 acaaccgaaa taccacgttt aattaataca gcaatcgccg aaagacgccc agttcattta
    97621 catttaccaa tcgatgtcgc aatctctgaa attgagatac cgacaccatt tgaagtgacg
    97681 gcaactaaat atacggatgc atcaacatat atagagttat tagcaactaa actgcatcaa
    97741 gcgaagcagc ctatcatcat tactggacat gaaattaaca gttttcacct ccatcaagaa
    97801 ttagaagatt ttgtaaatca aacacagata ccagtagcac aactttcatt aggaaaaggt
    97861 gcttttaatg aggaaaatcc atattatatg ggtatttacg atgggaaaat tgccgaagat
    97921 aaaatacgag attatgtgga caacagcgat ttaattttaa atattggagc caaattaaca
    97981 gattcagcaa cagcaggttt ttcataccaa ttcaatatcg atgatgtcgt tatgttaaat
    98041 catcacaata tcaaaattga cgatgttaca aatgatgaaa tatctctacc atcattgtta
    98101 aaacagttat ccaatatttc atatacgaat aacgcaacgt tccctgcgta tcatcgtcca
    98161 acatcacccg attatactgt tggcacagaa ccattaacac aacaaactta ttttaaaatg
    98221 atgcaaaatt tcttaaaacc aaatgatgtc atcattgctg atcaaggtac atcattcttt
    98281 ggtgcttatg atttagcatt atacaaaaac aatactttta tagggcaacc gttatggggt
    98341 tctatcggct atacattacc tgcaacatta ggttcacaat tagcagacaa agatcgtcgt
    98401 aacttattat taattggtga tggctcattg caactaactg ttcaagctat ttcaactatg
    98461 attagacagc atattaaacc ggtattattt gtgattaata atgacggcta tacggtagaa
    98521 cgacttattc acggcatgta tgaaccttat aatgaaattc acatgtggga ttataaagct
    98581 ttaccagctg tatttggtgg taaaaatgtt gaaattcatg acgttgaatc atcaaaagat
    98641 ttacaagaca cgtttaatgc aattaatggt catcccgatg tgatgcattt tgtcgaagtc
    98701 aaaatggctg tcgaagacgc accgaagaaa ctcatcgata tcgctaaagc tttttcacaa
    98761 caaaataaat aatttcatcg tatacagggt ataagtttaa gcgaatactt tattaaacga
    98821 ataggactct gatataagat gattaatttt aataaaaccg ctttagtgtt aatcgacctg
    98881 caagaaggta ttcttaaaat ggattatgcc ccatatacag ctgaaaatgt cgttcaaaac
    98941 gctaataaat taatagatgt ttttagaaaa aacaatggct ttatcgcttt tgttcgcgtg
    99001 aatttctatg atggtaaaga tgtattgcaa ccaaatgcaa tgatctcatt accaccaaaa
    99061 gaaggcgacg actacagtcg tttccatcat ttattagaca agagagatga cgattttgtc
    99121 atagacaaac gacattttag tgcatttgta ggaacagatt tggacttaca attgcgacgt
    99181 cgaggaattg atacgattgt tcttggtggt gtcgcaacgc atattggcgt agatacgaca
    99241 gcgcgagatg cctatcaatt aaactacaat cagttttttg ttacagatat gatgagtgca
    99301 caaaacgaaa cgctacatca atttccaata gataatgtat tcccattgat gggacaaaca
    99361 ataactacaa acgactttct aaatatattg aactaaacat atacttcccc ccttcgatca
    99421 tgttgagggg gatctttatt tcacaaagta ttaatacgtc gggttgtcta accttctata
    99481 tttaacatat tctatatctg ttaaatcgtt cttaacttac gcccctacta cataaaaaac
    99541 agtatttatt ccggaatttt caaaaaattt agt
//
LOCUS       DALAAR010000002        78029 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000002 DALAAR010000000
VERSION     DALAAR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 78029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.002"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            194..1141
                     /locus_tag="O2458_000085"
     CDS             194..1141
                     /locus_tag="O2458_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCZ3885977.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            1142..1609
                     /gene="ybeY"
                     /locus_tag="O2458_000086"
     CDS             1142..1609
                     /gene="ybeY"
                     /locus_tag="O2458_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCZ3885978.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            1612..1956
                     /locus_tag="O2458_000087"
     CDS             1612..1956
                     /locus_tag="O2458_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ3885979.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            1967..2371
                     /gene="cdd"
                     /locus_tag="O2458_000088"
     CDS             1967..2371
                     /gene="cdd"
                     /locus_tag="O2458_000088"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCZ3885980.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            2372..3271
                     /gene="era"
                     /locus_tag="O2458_000089"
     CDS             2372..3271
                     /gene="era"
                     /locus_tag="O2458_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCZ3885981.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            3299..4045
                     /gene="recO"
                     /locus_tag="O2458_000090"
     CDS             3299..4045
                     /gene="recO"
                     /locus_tag="O2458_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCZ3885982.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(4195..5586)
                     /locus_tag="O2458_000091"
     CDS             complement(4195..5586)
                     /locus_tag="O2458_000091"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCZ3885983.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            5921..6544
                     /locus_tag="O2458_000092"
     CDS             5921..6544
                     /locus_tag="O2458_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3885984.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6555..7373
                     /locus_tag="O2458_000093"
     CDS             6555..7373
                     /locus_tag="O2458_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCZ3885985.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            7434..9233
                     /locus_tag="O2458_000094"
     CDS             7434..9233
                     /locus_tag="O2458_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ3885986.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            9457..10563
                     /gene="rpoD"
                     /locus_tag="O2458_000095"
     CDS             9457..10563
                     /gene="rpoD"
                     /locus_tag="O2458_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCZ3885987.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            10694..11371
                     /locus_tag="O2458_000096"
     CDS             10694..11371
                     /locus_tag="O2458_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCZ3885988.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            11374..12474
                     /locus_tag="O2458_000097"
     CDS             11374..12474
                     /locus_tag="O2458_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCZ3885989.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            12588..13934
                     /locus_tag="O2458_000098"
     CDS             12588..13934
                     /locus_tag="O2458_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ3885990.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            13944..14834
                     /locus_tag="O2458_000099"
     CDS             13944..14834
                     /locus_tag="O2458_000099"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCZ3885991.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            14960..15745
                     /locus_tag="O2458_000100"
     CDS             14960..15745
                     /locus_tag="O2458_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ3885992.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            15787..16650
                     /locus_tag="O2458_000101"
     CDS             15787..16650
                     /locus_tag="O2458_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ3885993.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            16637..17047
                     /locus_tag="O2458_000102"
     CDS             16637..17047
                     /locus_tag="O2458_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ3885994.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            17324..17923
                     /locus_tag="O2458_000103"
     CDS             17324..17923
                     /locus_tag="O2458_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ3885995.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            18044..20119
                     /locus_tag="O2458_000104"
     CDS             18044..20119
                     /locus_tag="O2458_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCZ3885996.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            20232..20381
                     /gene="rpmG"
                     /locus_tag="O2458_000105"
     CDS             20232..20381
                     /gene="rpmG"
                     /locus_tag="O2458_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ3885997.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            20590..21129
                     /locus_tag="O2458_000106"
     CDS             20590..21129
                     /locus_tag="O2458_000106"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCZ3885998.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            21141..22604
                     /locus_tag="O2458_000107"
     CDS             21141..22604
                     /locus_tag="O2458_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCZ3885999.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            22585..22788
                     /locus_tag="O2458_000108"
     CDS             22585..22788
                     /locus_tag="O2458_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCZ3886000.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            22785..23771
                     /locus_tag="O2458_000109"
     CDS             22785..23771
                     /locus_tag="O2458_000109"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCZ3886001.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            23771..24100
                     /locus_tag="O2458_000110"
     CDS             23771..24100
                     /locus_tag="O2458_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCZ3886002.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            24097..24720
                     /locus_tag="O2458_000111"
     CDS             24097..24720
                     /locus_tag="O2458_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ3886003.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            24772..25746
                     /locus_tag="O2458_000112"
     CDS             24772..25746
                     /locus_tag="O2458_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCZ3886004.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            25718..26788
                     /locus_tag="O2458_000113"
     CDS             25718..26788
                     /locus_tag="O2458_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCZ3886005.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            26802..27113
                     /locus_tag="O2458_000114"
     CDS             26802..27113
                     /locus_tag="O2458_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ3886006.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            27091..27537
                     /locus_tag="O2458_000115"
     CDS             27091..27537
                     /locus_tag="O2458_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCZ3886007.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            27524..27823
                     /locus_tag="O2458_000116"
     CDS             27524..27823
                     /locus_tag="O2458_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCZ3886008.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            27741..28238
                     /locus_tag="O2458_000117"
     CDS             27741..28238
                     /locus_tag="O2458_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ3886009.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            28335..28481
                     /locus_tag="O2458_000118"
     CDS             28335..28481
                     /locus_tag="O2458_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886010.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            28471..28995
                     /locus_tag="O2458_000119"
     CDS             28471..28995
                     /locus_tag="O2458_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCZ3886011.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      28982..29079
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            29154..30245
                     /gene="gcvT"
                     /locus_tag="O2458_000120"
     CDS             29154..30245
                     /gene="gcvT"
                     /locus_tag="O2458_000120"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCZ3886012.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            30265..31611
                     /gene="gcvPA"
                     /locus_tag="O2458_000121"
     CDS             30265..31611
                     /gene="gcvPA"
                     /locus_tag="O2458_000121"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCZ3886013.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            31604..33076
                     /gene="gcvPB"
                     /locus_tag="O2458_000122"
     CDS             31604..33076
                     /gene="gcvPB"
                     /locus_tag="O2458_000122"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCZ3886014.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(33441..33827)
                     /locus_tag="O2458_000123"
     CDS             complement(33441..33827)
                     /locus_tag="O2458_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ3886015.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            33985..34815
                     /locus_tag="O2458_000124"
     CDS             33985..34815
                     /locus_tag="O2458_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ3886016.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(34879..35097)
                     /locus_tag="O2458_000125"
     CDS             complement(34879..35097)
                     /locus_tag="O2458_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886017.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(35111..35692)
                     /locus_tag="O2458_000126"
     CDS             complement(35111..35692)
                     /locus_tag="O2458_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886018.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            35797..36858
                     /locus_tag="O2458_000127"
     CDS             35797..36858
                     /locus_tag="O2458_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ3886019.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            36884..37441
                     /gene="efp"
                     /locus_tag="O2458_000128"
     CDS             36884..37441
                     /gene="efp"
                     /locus_tag="O2458_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCZ3886020.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            37915..38379
                     /gene="accB"
                     /locus_tag="O2458_000129"
     CDS             37915..38379
                     /gene="accB"
                     /locus_tag="O2458_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCZ3886021.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            38379..39734
                     /gene="accC"
                     /locus_tag="O2458_000130"
     CDS             38379..39734
                     /gene="accC"
                     /locus_tag="O2458_000130"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ3886022.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            39749..40111
                     /locus_tag="O2458_000131"
     CDS             39749..40111
                     /locus_tag="O2458_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ3886023.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            40171..40560
                     /gene="nusB"
                     /locus_tag="O2458_000132"
     CDS             40171..40560
                     /gene="nusB"
                     /locus_tag="O2458_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCZ3886024.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            40577..41914
                     /locus_tag="O2458_000133"
     CDS             40577..41914
                     /locus_tag="O2458_000133"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCZ3886025.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            41907..42137
                     /locus_tag="O2458_000134"
     CDS             41907..42137
                     /locus_tag="O2458_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCZ3886026.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            42115..42996
                     /locus_tag="O2458_000135"
     CDS             42115..42996
                     /locus_tag="O2458_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ3886027.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            43428..43880
                     /gene="argR"
                     /locus_tag="O2458_000136"
     CDS             43428..43880
                     /gene="argR"
                     /locus_tag="O2458_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCZ3886028.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            43896..45575
                     /gene="recN"
                     /locus_tag="O2458_000137"
     CDS             43896..45575
                     /gene="recN"
                     /locus_tag="O2458_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCZ3886029.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            45726..47147
                     /gene="lpdA"
                     /locus_tag="O2458_000138"
     CDS             45726..47147
                     /gene="lpdA"
                     /locus_tag="O2458_000138"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ3886030.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            47163..48155
                     /locus_tag="O2458_000139"
     CDS             47163..48155
                     /locus_tag="O2458_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCZ3886031.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            48155..49138
                     /locus_tag="O2458_000140"
     CDS             48155..49138
                     /locus_tag="O2458_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ3886032.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            49151..50425
                     /locus_tag="O2458_000141"
     CDS             49151..50425
                     /locus_tag="O2458_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ3886033.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            50776..51213
                     /locus_tag="O2458_000142"
     CDS             50776..51213
                     /locus_tag="O2458_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ3886034.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            51227..52207
                     /locus_tag="O2458_000143"
     CDS             51227..52207
                     /locus_tag="O2458_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ3886035.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(52334..52513)
                     /gene="prli42"
                     /locus_tag="O2458_000144"
     CDS             complement(52334..52513)
                     /gene="prli42"
                     /locus_tag="O2458_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCZ3886036.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            52776..53909
                     /locus_tag="O2458_000145"
     CDS             52776..53909
                     /locus_tag="O2458_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCZ3886037.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            53977..55383
                     /gene="gndA"
                     /locus_tag="O2458_000146"
     CDS             53977..55383
                     /gene="gndA"
                     /locus_tag="O2458_000146"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCZ3886038.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            55602..55973
                     /locus_tag="O2458_000147"
     CDS             55602..55973
                     /locus_tag="O2458_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886039.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            55912..56166
                     /locus_tag="O2458_000148"
     CDS             55912..56166
                     /locus_tag="O2458_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886040.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            56167..57186
                     /locus_tag="O2458_000149"
     CDS             56167..57186
                     /locus_tag="O2458_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ3886041.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            57202..58851
                     /locus_tag="O2458_000150"
     CDS             57202..58851
                     /locus_tag="O2458_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCZ3886042.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(58934..59800)
                     /locus_tag="O2458_000151"
     CDS             complement(58934..59800)
                     /locus_tag="O2458_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ3886043.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(60030..61514)
                     /locus_tag="O2458_000152"
     CDS             complement(60030..61514)
                     /locus_tag="O2458_000152"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCZ3886044.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            61827..62747
                     /gene="rnz"
                     /locus_tag="O2458_000153"
     CDS             61827..62747
                     /gene="rnz"
                     /locus_tag="O2458_000153"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCZ3886045.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(62852..63667)
                     /gene="proC"
                     /locus_tag="O2458_000154"
     CDS             complement(62852..63667)
                     /gene="proC"
                     /locus_tag="O2458_000154"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCZ3886046.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            63809..64564
                     /locus_tag="O2458_000155"
     CDS             63809..64564
                     /locus_tag="O2458_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ3886047.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            64580..64828
                     /locus_tag="O2458_000156"
     CDS             64580..64828
                     /locus_tag="O2458_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886048.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(65044..65952)
                     /locus_tag="O2458_000157"
     CDS             complement(65044..65952)
                     /locus_tag="O2458_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ3886049.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            66034..66576
                     /locus_tag="O2458_000158"
     CDS             66034..66576
                     /locus_tag="O2458_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCZ3886050.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            66681..67130
                     /locus_tag="O2458_000159"
     CDS             66681..67130
                     /locus_tag="O2458_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ3886051.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            67179..68066
                     /gene="xerD"
                     /locus_tag="O2458_000160"
     CDS             67179..68066
                     /gene="xerD"
                     /locus_tag="O2458_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCZ3886052.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(68144..68650)
                     /locus_tag="O2458_000161"
     CDS             complement(68144..68650)
                     /locus_tag="O2458_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCZ3886053.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            68742..69473
                     /locus_tag="O2458_000162"
     CDS             68742..69473
                     /locus_tag="O2458_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCZ3886054.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            69466..70008
                     /gene="scpB"
                     /locus_tag="O2458_000163"
     CDS             69466..70008
                     /gene="scpB"
                     /locus_tag="O2458_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCZ3886055.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            70001..70738
                     /locus_tag="O2458_000164"
     CDS             70001..70738
                     /locus_tag="O2458_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ3886056.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            70871..71596
                     /gene="srrA"
                     /locus_tag="O2458_000165"
     CDS             70871..71596
                     /gene="srrA"
                     /locus_tag="O2458_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCZ3886057.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            71577..73328
                     /gene="srrB"
                     /locus_tag="O2458_000166"
     CDS             71577..73328
                     /gene="srrB"
                     /locus_tag="O2458_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCZ3886058.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            73573..74502
                     /locus_tag="O2458_000167"
     CDS             73573..74502
                     /locus_tag="O2458_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ3886059.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            74495..76521
                     /locus_tag="O2458_000168"
                     /pseudo
     CDS             74495..76521
                     /locus_tag="O2458_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(76564..77769)
                     /locus_tag="O2458_000169"
     CDS             complement(76564..77769)
                     /locus_tag="O2458_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ3886060.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            77895..>78029
                     /locus_tag="O2458_000170"
     CDS             77895..>78029
                     /locus_tag="O2458_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ3886061.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     I"
BASE COUNT        28464 a        10618 c        14276 g        24671 t
ORIGIN      
        1 gcaaaaaatt gattagtcta aaattgaatc tgcttttatg acaaggtgaa aagtataaat
       61 gattatttta aattaaagaa aaatgagtaa gtcaatgcaa agatgtttaa atcaatcaat
      121 tgcatgatat aattaagtag atattaaagc atcatagaat gaatataaat gatatatgaa
      181 aaggagcgcg tgtatgcctg gaattataca aatagacgat atgaaccaat ctcaagcttt
      241 aattggaaat aatgatgaac atttaaaagc aattgaagag agtttcgatg ttgtcatcca
      301 tgcaagagga caagaagttg ccgttaaagg tacaaaaata gaaaacgtag aaaaagcgga
      361 atcagtatta atcaatttgc tgaaggttat tgatttaggt aataatatta caattaaaga
      421 tgttgaagca gctattaaaa tggcgcataa taacacaatt caacatctgt tagatttata
      481 tgatgaagag ataactaaag atgcatttgg taagacgatt cgtgcgaaaa cgatggggca
      541 acgtatatat gttaatgcca tgaaaaataa tgatttagta tttggtatag gtcctgctgg
      601 tacaggtaag acattcttag ctgtagttta tgcagcaaag caactccgta aaggtgctgt
      661 taaacgtatt gtattaacaa gacctgctgt tgaagcagga gagtcacttg gatttttacc
      721 aggagatttg aaagaaaagg tagatccata tttaagacct ttatatgatg gtctatatac
      781 tgttcttggg cgtgaacaaa cagagcgatt tattgaaaga ggcattatcg aaatagcgcc
      841 acttgcatat atgcgcggac gaacattaga agatgcattt gtaattcttg atgaggcgca
      901 gaatacgaca catgcgcaaa tgaaaatgtt tttaacaaga ctaggttttg gctcaaaaat
      961 ggtagttact ggtgaccaaa ctcaaatcga tttacctaaa ggtgttaaaa gtggacttaa
     1021 ggaagcggtc agtaggttac acaacgttaa aggtataagt atattgaaat tagatcagag
     1081 cgatgtagtc agacatccat tggtaagtaa gatcattgaa cattatgaag gagagaatta
     1141 aatgtttacg atagatttta gcgatcacac aggcttagtt aaagatgctt ggtataaaca
     1201 aattgaagat ttattagaat ttgctaaaaa agaagagcat atagaagacg atgctgagct
     1261 ttctgttaca tttgtagata aacaagaaat acaagaaatt aatcgaacat atagagataa
     1321 agataaagtt acagatgtaa tctcatttgc tttagaagaa gatgagccag agattgattt
     1381 tagtggtctt gatataccac gtgttttagg ggatataatt atctgtacgg atgtagcgca
     1441 agaacaagca aacaattacg gacattcttt tgaacgagaa ttaggatttt tagcattaca
     1501 tggatttttg catctattag gttatgatca tatgactgaa gcggatgaaa aggaaatgtt
     1561 tggtcgacaa gatacaatat taaacgcata tggattaaca cgagactaat tatgaaaagg
     1621 tttaaatatg cacttgatgg gctgaaaatc ttaattcaaa aagactataa atttctttta
     1681 catgtgtttg caatgattgt tgctattgtc tttggtctcg tactaaatat taatcggatt
     1741 gagtggatat ttatactcat tgctattgca ttagttctca ctgttgaagc tttaaacact
     1801 gctattgaat atgttgtcga tttagtgacc gttgaatatc atgatttagc taaatacgct
     1861 aaagatattg cggcttttag tgtacttata gtttcaatat tagcatttat tataggttta
     1921 atagtatttt taccacattt tatagcgtta ttttagggag gcatatatga gttatcaacc
     1981 tcattatttt caagaagtta gaaaagcaca acaagaatca tattcgccat acagtcaatt
     2041 taaagtaggg gcttatttaa aaacgaaaga cggtagaact ttttatggta ccaatgtaga
     2101 aaatgcttct tatccattat cgatatgtgc tgaacgagct agtttggtat cggcaatttc
     2161 tcaaggatac agaccaggtg attttgaatc aataactgta accgtagatg cagataaacc
     2221 gtcatcacct tgtggtgcat gtcgtcaagt tttgaaggaa ttatgtgatg atgatatgcc
     2281 tgtgtatatg acaaatcata aaggagatat ggttatgatg acagtcgcag agttactacc
     2341 atttggattt tcaggaaagg atttagaata aatgacagaa cataaatcag gatttgtttc
     2401 aattataggt agaccaaatg taggaaagtc aacatttgtt aatagagtga tcggccataa
     2461 aatagcaatc atgtccgata aagctcaaac aactagaaat aaaattcaag gtgttatgac
     2521 aagagatgac gcgcaaatta tattcattga tacgccaggt attcataaac ctaaacacaa
     2581 attaggtgac tatatgatga aagtcgctaa aaatacatta tctgagatag atgcaatcat
     2641 gtttatggtt aatgccaatg aggaaattgg acgaggcgat gaatatatta tagaaatgtt
     2701 gaaaaatgtt aagacaccag tatttttagt attaaataaa atagatttag tgcatccaga
     2761 tgaattaatg ccaaagattg aagaatatca aagttatatg gactttacag agattgtacc
     2821 tatttcagca ttagaagggc taaatgtcga tcattttatt gatgttttaa agacgtattt
     2881 acccgaagga cctaaatatt atccagatga tcaaatttca gaccatcctg aacaatttgt
     2941 agtgggtgaa atcattcgtg aaaaaatcct tcatcttaca agtgaagaaa tccctcatgc
     3001 gattggtgtt aatgtggacc gtatggttaa agaaagcgaa gatcgtgttc atatcgaagc
     3061 aactatatat gttgaaagag attcgcaaaa aggaattgtc attggaaaag gcggtaaaaa
     3121 gttaaaagaa gtaggaaaac gtgcgagacg tgatatagaa atgcttctag gctctaaagt
     3181 ttacttagaa ttatgggtca aagttcaaag agactggcga aacaaagtta actttattcg
     3241 ccaaattggt tatgttgaag accaagatta atcttaaaag tggtgaagat aattgttaat
     3301 gcgccaaaaa gggattatca tcaaagcagt tgattatggt gaatctgata aaattatcac
     3361 gattttaaat gagcatggtg caaaagtacc acttatggca aggcgtgcta aaaaagttaa
     3421 gacgggttta caagcgcaaa cgcaattgtt tgtttatggt ttgtttattt acaatcagtg
     3481 gcgaggtatg ggaacgttaa attctgtaga tgttattagt caacattata aattacaaat
     3541 ggacctttac gtaagcagtt atgccgctct ggcagctgaa actattgagc gctcaatgga
     3601 tgaaggtgac attgcaccat ataactatca attattacaa tttgttcttg aaaaaataga
     3661 atcaggtaca tctgcacagt taatgtcagt cgtagttatg ttaaagtgca tgaaacgatt
     3721 tggttttact gcatcattta atcgctgtgc tgtgagtggt aatgacacac aagcagattt
     3781 aataggttat agttttaagt ttgacggtgc gatttcaagg caagaggctt ctaaagatgt
     3841 acatgcagtt atattatcga ataaaacact atatttatta gatgtattac aaaaattacc
     3901 gatagataaa atgaattcat tgaatatcca tcaagaaatt attgatgaaa tgtcagatat
     3961 cattttaatg ttatatcgtg aatatgcagg tatgtttttt aaaagtcaga aactaatcaa
     4021 ccaattaaaa agattggaac aataacataa aataataaaa ggtattcaag tagccacata
     4081 gatgtgttta tttgaatacc tttttgaata gaaaagagat actggcaatt ttactaacca
     4141 gtatctcttt ttataaatta tatagccacc acatatggtg gaaagtcttt ttaattagaa
     4201 ttttgttttt tcagttaaga aagcttctaa ctctgagatt ggcatacgaa cttgttccat
     4261 tgaatctctg tcacgtactg taacttgatt atcttctaat gaatcaaagt cgaatgttac
     4321 acaataaggt gtaccgattt catcttgacg acggtatctt ttaccgatag attgtgattc
     4381 atcgaaatcg attgagaatt tagaacttaa ttgctcaaaa atcttaatcg cttcgccaga
     4441 taatttctta cttaaaggta aaatcgctgc tttatatggt gctaatgcag gatggaagtg
     4501 taaaactgta cgtgcatctt tactaccttc aacgccttct tcatcatatg catcacataa
     4561 gaaagctaat gttacacgat ctgcaccaag tgatggctcg atacaatatg gaatatattt
     4621 ttcgttcgtt tctggatcat ggtatctgaa atcttcacca gagtgttcag catgtttacg
     4681 taagtcgaag tctgtacgac ttgcgatacc ccataactca ccccaaccaa atgggaattt
     4741 atattcaata tcagttgttg catttgagta atgagataat tcatcttcat catgatcacg
     4801 taaacgcata ttttcactgc tcatatttaa gcttgttaac cagtcacttg caaaagtttt
     4861 ccaataattt tgccattcga tttcttctcc aggtttacag aagaattcaa gttccatttg
     4921 ttcaaattct cttgttctga aaatgaagtt acctggagtg atttcattac ggaatgattt
     4981 accaatttga ccgataccaa atggtaattt tttacgcatt gaacgttgca cgtttttata
     5041 gtttacaaaa ataccttgtg ctgtttcagg acgtaagaaa atttcatttg tagaatcttc
     5101 agttacacct tggaatgttt taaacattaa attgaattgg cgaatttcag tccagttagc
     5161 agttttactt acaggacaaa caataccttc atcgtcaata atttttttca tttgttcaaa
     5221 acttaaacca tcggcaatga agttttcatc acctttaaca tcttgcatat aatcttcaat
     5281 taatttatca gcgcgatatc gaattttact atctttatta tcaatcattg ggtcgttgaa
     5341 gttgtttaag tgtcctgaag cttcccatac ttttggattc attaagattg cagcatcgat
     5401 accaacgtta aacggtgatt gtgtaatgaa tttttgccac caagcttttt taacattatt
     5461 ctttaattca acacctaaag gaccataatc ccatgtgttt gataaaccac cgtaaatatc
     5521 actaccaggg aacacaaaac ctctgtgttt tgctaatgaa acaattgtat ccatatcttt
     5581 tgccataatt acctctcctc atacatgaaa acgccccatg gaaaataaca gcatagtaaa
     5641 tatgctttac ttccatggga cgagttaata ttttaaattg tatataatac aaaataagtt
     5701 acgtatttaa cccgcggttc cacccaaatt agtgtagtca ctcgctttta ttttaaaatg
     5761 attcgttgcg ccaatcttat tgttaagctt acactatcct taactcgctg tacgtttatt
     5821 ctaattgata gattatttaa aggcaagtaa aattaatgtg tttgtgacat gaaagaaata
     5881 tcggtataat aataggtgaa taaaaaaggg gtgaagacct atagaactca gtcaaagaca
     5941 agaacgaatc atcgaaattg ttaaaactaa aggacccatt actggtgaac aaatagcaga
     6001 taagttgaat ttaacaagag caacgcttag accagattta gcgatattaa caatgtcagg
     6061 ttttttagaa gcgcgtcccc gagttggata ttattattca ggtaaatcaa aaggcaaatt
     6121 ttttaatgaa aaacttcgac aatttgaggt taaggactat atgtctcaac ctgttgtgct
     6181 aagagagaat acaacagttt atgacgctat ttgtacaatt tttttagaag atgtaagtac
     6241 attatttatt attaatgaag ataacgattt tgttggtgtg tgttcaagaa aagatttatt
     6301 aagagcttca atgattggag cagatattca taaagtacct atcagtgtaa atatgacacg
     6361 tatgcctaat gtcacttatt tagaggaaag cgaattagtc atatacgcag cagatagaat
     6421 gattgaaaaa gaaattgatt cgattccaat tgtaagaaaa aaagataatc aaaagtatga
     6481 agtaattgga agaatttcca aaacaacaat agctaagtta ttagtagcat tatataaaga
     6541 ataggtgaga agtaatggaa aaaattaaaa ttatcgtagc ttcagattct ataggtgaaa
     6601 cggcagagtt agttgctagg gcaggtattt cacaattcaa tcctaagcaa tgtaaaaatg
     6661 aattattaag atatccatat attgaatctt ttgaagatgt tgatgaagtg attcaagttg
     6721 caaaagatac aaatgctatc attgtttata cacttattaa acctgaaatg aagcaatata
     6781 tgagtgagaa agtagcagaa ttccaattga agtctgtcga tatcatgggg ccattaatgg
     6841 atttattatc tgcttcggtt gaagaaaaac cttataatga gccaggtatc gttcatagat
     6901 tagatgatgc atatttcaag aaaattgatg cgatagagtt tgcagttaaa tatgatgatg
     6961 gtaaagatcc taaaggatta cctaaagctg atattgtttt acttggtatt tcgagaactt
     7021 caaagacacc attatctcag tatttagcgc ataagagtta caaagttatg aatgtaccga
     7081 ttgtaccaga agtgacaccg ccagatggct tatatgatat taatccaaag aaatgtatcg
     7141 cacttaaaat aagtgaagaa aaattaaatc gcattagaaa agagcgacta aaacaattag
     7201 gactaggtga cacagctcga tatgcaacag aagcacgaat tcaagaagaa ttgaattact
     7261 ttgaagaaat cgtaagtgaa attggatgtc ctgtcattga tgtttctcaa aaagcaatcg
     7321 aagaaacagc aaacgatata atccattata ttgaacaaaa taaatcgaaa tgatttcatt
     7381 tttgtcgaaa attaggtata atagtataac taatgcttaa taggtgattt aatttgcgaa
     7441 tagatcaatc gatcattaat gaaataaaag ataaaaccga cattttagac ttggtaagtg
     7501 aatatgtaaa attagaaaag agaggacgca attatatagg tttgtgtcct tttcatgatg
     7561 aaaagacacc ttcatttaca gtttctgaag ataaacaaat ttgtcattgt tttggttgta
     7621 aaaaaggtgg caatgttttt caatttactc aagaaattaa agacatatca tttgttgaag
     7681 cggttaaaga attaggtgat agagttaatg ttgctgtaga tattgaggca acacaatcta
     7741 actcaaatgt tcaaattgct tctgatgatt tacaaatgat tgaaatgcat gagttaatac
     7801 aagaatttta ttattacgct ttaacaaaga cagtcgaagg cgaacaagca ttaacgtact
     7861 tacaagaacg tggttttaca gatgcgctta ttaaagagcg aggcattggc tttgcacccg
     7921 atagctcaca tttttgtcat gattttcttc aaaaaaaggg ttacgatatt gaattagcat
     7981 atgaagccgg attattatca cgtaacgaag aaaatttcag ttattacgat agatttcgaa
     8041 atcgtattat gtttcctttg aaaaatgcgc aaggaagaat tgttggatat tcaggtcgaa
     8101 catataccgg tcaagaacca aaatacttaa atagtcctga aacacctatc tttcaaaaaa
     8161 gaaagttgtt atacaactta gataaagcgc gtaaatcaat tagaaaatta gatgaaatcg
     8221 tattactaga aggttttatg gatgttataa aatctgatac tgctggcttg aaaaacgttg
     8281 ttgcaacaat gggtacacag ttgtcagatg aacatattac ttttatacga aagttaacat
     8341 caaatataac attaatgttt gatggggatt ttgcgggtag tgaagcaaca cttaaaacag
     8401 gtcaaaattt gttacagcaa gggctaaatg tatttgttat acaattgcca tcaggcatgg
     8461 atccggatga atacattggt aagtatggca acgatgcatt tactgctttt gtaaaaaatg
     8521 acaaaaagtc atttgcacat tataaagtga gtatattaaa agatgaaatt gcacataatg
     8581 acctttcata tgaacgttat ttgaaagaac taagtcatga tatttcgctt atgaaatcat
     8641 cgattttgca acaaaaggct ttaaatgatg ttgcaccatt tttcaatgtt agtcctgagc
     8701 aattagctaa cgaaatacaa ttcaatcaag caccagccaa ttattatcca gaagatgagt
     8761 atggcggtta cattgaacct gagccaattg gtatggcaca atttgacaat ttgagccgtc
     8821 aagaaaaagc ggagcgagca tttttaaaac atttaatgag agataaagat acatttttaa
     8881 attattatga aagtgttgat aaggataact tcacaaatca gcattttaaa tatgtattcg
     8941 aagtcttaca tgatttttat gcggaaaatg atcaatataa tatcagtgat gctgtgcagt
     9001 atgttaattc aaatgagttg agagaaacac taattagctt agaacaatat aatttgaatg
     9061 acgaaccata tgaaaatgaa attgatgatt atgtcaatgt tattaatgaa aaaggacaag
     9121 aaacaattga gtcattgaat cataaattaa gggaagctac aaggattggc gatgtagaat
     9181 tacaaaaata ctatttacag caaattgttg ctaagaataa agaacgcatg tagcatgtga
     9241 ttttaaagaa taatacgaat aatgattatg tcaaaatgta taagggtaaa tgatagttac
     9301 cgcatttaaa caacactatt gaaaaataaa tattgggatt agttccaatt tgtaaaataa
     9361 aattaaaaat atggatgaat taattaagaa tttagtttaa aatagcaata ttgaataaat
     9421 ttcgaatgtt catatttaaa atcgggaggc cgtttcatgt ctgataacac agttaaaatt
     9481 aaaaaacaaa caattgatcc gacattaaca ttagaagatg ttaagaagca attaattgaa
     9541 aaaggtaaaa aagagggtca tttaagtcat gaagaaattg ctgaaaaact tcagaatttt
     9601 gatatcgact ctgatcaaat ggatgatttc tttgatcaat taaatgataa tgatatttca
     9661 ctagttaatg aaaaagatag ttcagatact gacgagaaac tgaatccaag tgatcttagt
     9721 gcccctccag gtgttaaaat aaatgaccca gttcgtatgt accttaaaga aattgggcgt
     9781 gttaacttat taagtgcaca agaagaaatc gaattagcca aacgtattga acaaggtgat
     9841 gaagtagcaa aatcaagact tgcagaagcg aacttacgtt tagttgtaag tattgctaaa
     9901 agatacgtag gtcgtggtat gttattcctt gatttaatcc aagaaggtaa tatgggtctt
     9961 attaaagctg ttgaaaaatt tgactttaac aaaggattta agttttcaac atatgcaaca
    10021 tggtggatta gacaagcaat cactcgtgca attgctgacc aagcacgtac gattcgtatc
    10081 cctgtgcata tggtagaaac aattaataaa ttaattcgtg ttcaacgtca attattacag
    10141 gacttaggtc gagatccagc accagaagaa attggtgaag aaatggattt accagcagaa
    10201 aaagttcgtg aaattttaaa aattgcgcaa gaacctgttt cattagaaac accaattggt
    10261 gaagaagatg atagtcattt aggagacttt attgaggatc aggaagcaca aagtccttca
    10321 gatcatgctg cttatgaatt attaaaagag caattagaag atgtgcttga tacattaact
    10381 gatagagaag aaaatgtatt acgattaaga tttggtcttg atgacggcag aacaagaaca
    10441 cttgaagaag ttggtaaagt tttcggtgtt acacgtgaac gtattcgaca aattgaagca
    10501 aaagcactta gaaaattaag acatccaagt cgtagtaaac gtttgaaaga ctttatggat
    10561 taatttatat caattttaat gattgatacc aagacatgaa taagcgctta tttattttta
    10621 gattaagatt taacttgagt gaggttggta cattgaataa tgttcaacct cttttttcat
    10681 aaaggagaat aaaatgattt cgttaaataa ccgattaacg acggtaagtc gatttttaaa
    10741 acaaggtaca atcgcggata ttggctcaga tcatgcttat ttgccaatct atgcaattca
    10801 aaaccattta tgcgaatgcg gtattgctgg agaagttatt caaggccctt ttcaagctgc
    10861 tgtgaaaaat gtagctgcaa accaattagt agatagaatt gatgtacgtt taggggatgg
    10921 cttaagtgtt attcaacctg aggacgtaat cgataatatc acaatttgtg gtatgggcgg
    10981 accacttatt gctaaaattt taaaagatgg gcaagataag ttaagtcaac atccaaggtt
    11041 gattttacaa agtaatatcc aaactgaaaa tttaagacaa acattacaac aattgaatta
    11101 tgaaataatt gatgaaataa taatggaaga aaaaggccat atttatgaaa ttgttgtagc
    11161 agaatattct acagaattaa tagagctatc atctgatgaa ttgaaattcg gaccaaaatt
    11221 gctgaacaac aaaaatgaat attttataaa aaaatggcaa agagagttag aagcacttta
    11281 tcatattaaa tcgaaactca atactgaaca acatcatcaa cgtttagctc aaattaatga
    11341 cgaaattgct gtaattgaaa gggtgttata gtaatgaaaa tagctgattt aatgacattg
    11401 ttagatcatc atgtgccatt tagtactgct gaatcttggg ataatgtagg attgttaata
    11461 ggtgatgaag atgttgaagt tactggtgtt ttaacagcat tagactgtac gttggaagta
    11521 gtaaatgaag caatcgaaaa aggttataat actattatta gtcatcatcc tctaatcttt
    11581 aaaggcgtaa catcattaaa agctaatggt tatggtttga tcattagaaa actaattcaa
    11641 catgacatta atttaatagc gatgcataca aatttagatg taaatccgta tggtgtcaat
    11701 atgatgttgg cgaaggcgat gggtttgaag aacatttcaa taataaataa tcaacaagat
    11761 gtatactata aagttcaaac atatatacct aaggataatg ttggaccatt taaagataag
    11821 cttagtgaaa atggattagc gcaagaaggt aattatgaat attgtttctt tgaaagtgaa
    11881 ggaagagggc aattcaaacc agttggtgaa gctaatccaa caataggaca aattgataaa
    11941 attgaatatg tagatgaagt taaaattgaa tttatgatag atgcatatca aaagtcaagg
    12001 gctgagcaat taattaaaca ataccatcca tatgaaacac cggtatttga ttttattgag
    12061 ataaaacaaa catcccttta tggacttggc gttatggcag aagtggataa tcaaatgaca
    12121 ttggaagatt tcgcagctga tattaaatct aaattaaata tcccaagtgt ccgttttgtt
    12181 ggtgagtcta atcagaaaat taaacgtatt gcaattattg gtggttcagg tattggatat
    12241 gaatatcaag ctgtccaaca aggcgcagat gtctttgtta caggtgatat taaacatcat
    12301 gatgccttag atgctaaaat tcatggtgtg aatttaattg atattaatca ttacagcgaa
    12361 tatgtgatga aagaaggttt aaaagcgtta ctaatgaatt ggttcaatac agaaaaaata
    12421 aatttagatg ttgaagcatc tacaattaat acagatccat ttcaatatat ttaaactaaa
    12481 atctcgggat ttctaaattt tgaaatttcg aggttttaat atttttattt aaaatagcac
    12541 atttatactt tataatagta aagatgaaca tataaggagg ccaaatcatg gcaaaacatc
    12601 cattcgaaca atttaatcta gaatctagtt taattgacgc tgtgaaagac cttaattttg
    12661 aaaaaccaac tgaaattcag aatcgaatta ttccaagaat actaaagaga acaaatttaa
    12721 ttggtcaatc tcaaacgggt acagggaaat ctcatgcatt tttattacca ttaatgcagt
    12781 taattgatag tgaaataaaa gaaccacaag caatcgtagt tgcaccaaca agagaacttg
    12841 cacaacaact atacgatgca gcgaaccatt taagccaatt taaagctggt gtttcagtta
    12901 aagtttttat tggtggtaca gatatagaga aagatagaca acgttgtaat gcacaaccac
    12961 aattgattat aggcacccct actagaatta atgacttagc taaaacggga catttacatg
    13021 tgcacttagc atcatattta gttattgatg aagcggatct tatgattgac ttaggattaa
    13081 ttgaagatgt agattacatt gctgcaagat tggaagataa tgcaaatatt gcggtgttta
    13141 gtgctacaat cccacaacag ttacaaccat ttttaaataa atatttaagt catccagaat
    13201 atgtagctgt cgacagtaaa aaacaaaata aaaagaacat cgaattctat ttaataccta
    13261 ctaaaggtgc agctaaagtt gaaaagactt taaatttaat tgatatacta aatccatact
    13321 tatgtattat tttctgtaat agtagagata atgcaaatga tttagcacgt tcactaaatg
    13381 aagctggtat taaagttggt atgattcatg gtggcttaac gccacgtgaa cgtaaacaac
    13441 aaatgaaacg tatacgtaat ttagaattcc aatacgttat tgccagcgat ttagcatctc
    13501 gtggtattga tattgaaggt gttagtcatg tcatcaattt tgatgtgcca aatgatattg
    13561 acttctttac gcatagagtc ggacgaactg gtcgtgggaa ttataaaggt gtagcaatta
    13621 cgctttatag tcctgatgaa gaacacaata tttcattaat agaagatcgc ggttttgtat
    13681 tcaatactgt tgatattaaa gatggtgagt taaaagaagt taaagcgcac aatcagcgtc
    13741 aagcaagaat gcgcaaagat gaccatttaa ctaatcaagt gaagaacaaa gttcgaagta
    13801 aaattaaaaa caaagttaaa ccaggttata agaagaaatt taaacaagaa gttgaaaaaa
    13861 tgaaacgtca agagcgtaag caatttagta agcagcaaaa tagacaaaaa cgtaagcaaa
    13921 acaaaaaagg ttaggtgtgt tttatgttat taggatcaca tgtttcaatg agtggtaaaa
    13981 agatgttaga aggttctgct atagaagcgc atgaatatgg tgaaacaaca tttatgattt
    14041 atactggtgc acctcaaaac acgcgccgta aaagtattga agatttaaat ataactaaag
    14101 gtcatgaagt gatggaaaaa tatggtttat ctaatattgt tgttcatgca ccatacatca
    14161 ttaatattgc aaatacaacc aaaccagaaa cgtttaatct tggtgtagat ttcttgcaac
    14221 aagaaattga aagaacgcaa gctatagggg ctaaagatat tgtattacat ccaggtgcgc
    14281 atgttggagc tggtgttgat gccggaatta ataagattat tgaaggatta aatgaagttt
    14341 taacgaatga caataatgta cgtattgcgc ttgaaacgat ggcaggtaaa ggtacagaga
    14401 ttggtcgctc atttgaagag ttagcacgta ttattgatgg agttcataat aacgaacgtt
    14461 tatcagtatg ttttgataca tgtcatacgc atgatgctgg atataatgtt aaagaggact
    14521 ttgatggcgt attaaatgaa tttgataaaa tcattggagt cgacagaatc aaagttgttc
    14581 atgtcaatga ttctaaaaat gaccgtggcg ctcaaaaaga ccgacatgaa aatattggct
    14641 tcggttacat tggttttgat gcattaaatt acattgtgca tcatgatagt tttaaagata
    14701 ttccgaaaat cttagaaaca ccttatgttg gtgaagataa gaaaaataaa aaaccgccat
    14761 ataaacttga aattgaaatg ttaaaacaac agcaatttga cccagaatta aaaaataagg
    14821 ttatgcaaca ataaaatgat aatgctctct tcaagaaatg cgataaataa aacttgaagg
    14881 gggcatatct ctttattttg tctaattttg agtcgtaaac attactgttt acatatgtta
    14941 ctaggagttg tatagttgaa tgcgaggtga gaatacgatg acaccagtct ttgaattgaa
    15001 aaatgtcaat tactactatg atcataaaaa agtgttagaa aatataaaca ttaaaataaa
    15061 taaaggtgaa tttttagcaa ttgttggacc aaatggtgct ggtaaatcaa cattattgaa
    15121 gttgattcta gggttattac ctttacaaag tggtgagatt tttgttgaag gtattgattt
    15181 taaaaataag aaaacatcaa ttaaattaag ctatgtatca caaaaagcaa atgcctttaa
    15241 ttcaggtttc ccagcaagtg ttaaagaagt tgttttaagc ggattaacaa agacaaaacg
    15301 tcttttccaa acatttaata gcaaagataa tgaaaaagtg attaaagtac tagaaagact
    15361 gaatataagt gatttaattc ataaaaatat agcagaatta tcaggtggtc aacaacaacg
    15421 tgtaatgatt gctcgagcat tgatttcaga acctgcagta ttagtacttg atgaaccaac
    15481 gaatggtatt gatgcaaaac atgtaagtga attttataat actttagatc aattaaaaca
    15541 agaaggtatc accattatct tagttactca tgatatcggt gttgtagcag atactgctac
    15601 tgaagtagca tgtttaaata agcatttgca tttccatggt acaactgatg agtttaaatc
    15661 acttgatgaa gttgaaattt caaaaattta tggacatcct gtacgttttg tcgatcatca
    15721 gcataatcga gaatgttgta attaataaag atttgagttt atttaaaatg gaaaggcgtg
    15781 tcacaattga tagatgcatt attgaatttt gattttatga ggtactcttt aattagtggt
    15841 atcttgatag gttttattgc gcctctaatc ggtgctttta tcgttgttag acgactatca
    15901 cttatagctg atgctctaag tcatgtaact ttaggtggta tatctttcgg tatgttttta
    15961 cttactatta tgccaacact agtatttatt aatccaatgt ggtttggaat cttattcgca
    16021 atagtaggtg cgcttctaat tgaaaaatta agaacgtcat acactgctta ccaagaaatt
    16081 gctattccaa ttataatgag tgctggtatc gccttgagtg caatcttcat ttcattagct
    16141 gatgggttca atcaagaaat tgttggattg ctatttggct caataagcgc agtgaatata
    16201 agtgatttaa caacgattat tgtcattgcc atcatagttg ttctgtttat tacattgttt
    16261 tataaagaat tatttatatt gtcatttgat gaggaatata gtaaagtcat tggcattcct
    16321 aaatggattc aatttttatt tattgttatt gtagcgatgg ttatttctgc ctcaatgcgt
    16381 gttgtaggga tactgttagt aagtgcgtta atcacgttac caattgcaat aagtatgcga
    16441 ataacaaaaa gttttaagca attgatatta ctaagtgtat ttttaggtga attatcagta
    16501 atattaggct tagtcctagc tttctatatg gacatttctc cgggtggtgt aattgtagtt
    16561 ctacttgtta ttttacttat gattacaatg gcttatcaga aaatgcgaat gaagtttaaa
    16621 aagggagcta atatcaatga atacaaatga tgctattaaa attttaaaag agaacggttt
    16681 aaaatataca gataaacgta aagatatgtt agatattttt gtcgaagaag ataagtatat
    16741 aaacgcaaag tatatacaac aagttatgga tgaaaattat cctggaattt cattcgacac
    16801 aatatataga aacctgcact tatttaaaga tttaggaatt attgaaaata cagaacttga
    16861 tggtgaaatg aagtttagaa tcgcttgtac aaaccatcat catcatcatt ttatctgtga
    16921 aaagtgtgga gatacaaagg taatagatta ttgtccaata gatcagataa aattatcact
    16981 acctggtgtt aatattcaca aacacaaact tgaagtttat ggtgtatgtg agtcttgcca
    17041 agattaatat aaagaaatga gatttatgca catttggtca gatgtatgca taaatctcat
    17101 ttctttatat tatagaagat aaaggttcat ttcatactcc gttcgtatga ttttatggca
    17161 aatctgttaa ctttttatat acaaaatgtt taattatttt tgatttgagt tatattaaat
    17221 gagtagagaa aataataagt agtagaattt aagcaaattc tttgttgaac cagtcactgc
    17281 ttgttattat ttacttacag acattttagg aggatgatta tttatggctt ttgaattacc
    17341 aaaattacca tacgcatttg atgcattaga accacatttt gacaaagaaa ctatggaaat
    17401 tcaccatgac agacatcata acacgtatgt tacgaaatta aatgctgcag tagaaggtac
    17461 agatttagaa tctaaatcta ttgaagaaat tgttgctaat ttagacagtg taccagctaa
    17521 catccaaact gctgtacgta ataatggcgg tggacattta aaccattcat tattctggga
    17581 gttactttca ccaaactcag aagaaaaagg tactgtagta gaaaaaatta aagaacaatg
    17641 gggttcttta gaagaattta aaaaagaatt tgctgacaaa gcagctgcac gctttggttc
    17701 aggttgggct tggttagtcg taaacaatgg ccagttagaa attgtgacta caccaaacca
    17761 agataatcca ttaactgagg gtaaaacacc tattttaggt ttagacgtat gggaacacgc
    17821 ttattaccta aaatatcaaa acaaacgccc tgactacatt ggcgcatttt ggaatgtagt
    17881 taactgggaa aaagttgacg aattatataa tgcaacaaaa taaagtatga ttacttgttc
    17941 ggtctcttaa atgagaccgt tatttttttg tcaaaaagat agaaataatt tctaaattca
    18001 tatatgattt aaagtgaaag actttgaata gaggtaggta gttttgttaa aaagactaaa
    18061 agaaaaatca aatgatgaaa tcgttcaaaa tacaattaac aagagaatta actttatatt
    18121 tggtgtgatt atatttattt ttgcagtact agtactacgt ttaggttatt tacaaatcgc
    18181 acaaggctca cattataaac aaattataaa aaatgatgaa aacattacag tgaatgagtc
    18241 tgtgccaaga ggtcgtattt tagacagaaa tgggaaagtt ttagttgata atgcttctaa
    18301 aatggctatt acatatacta ggggtcgaaa aacaacacaa tcggaaatgt tggatacggc
    18361 tgaaaagtta tcaaagctaa tcaagatgga tactaagaaa attacagaac gtgataagaa
    18421 agatttctgg attcagttgc atcctaaaaa agcaaaagca atgatgacaa aagaacaagc
    18481 tatgttagca gatggaagta ttaaacaaga tcaatatgat aaacaactgt tatcgaaaat
    18541 cggaaaatca caattagatg aattgtcttc taaagattta caagttttag ctatttttcg
    18601 agagatgaat gcaggaacag ttttagatcc acaaatgata aaaaatgaag atgtcagtga
    18661 aaaagagtat gcagcagttt ctcagcaact ttccaaatta ccaggtgtta acacgtctat
    18721 ggattgggat agaaaatatc catatggcga tactttaaga ggtatattcg gagatgtatc
    18781 gacacctgct gaaggtattc caaaagaatt gacagaacat tacttatcca aaggatattc
    18841 acgcaatgat cgtgttggaa aatcttacct agaatatcaa tatgaagatg tattgcgtgg
    18901 taagaagaaa gaaatgaaat acacaacgga caaatctggt aaagttacat cttcagaagt
    18961 gttaaatcct ggcgctcgcg gtcaagattt gaaattaacg atcgatatag atcttcaaaa
    19021 agaagtagaa gcattattag ataaacaaat taagaagctt cgcagtcaag gtgccaaaga
    19081 tatggataat gcaatgatgg ttgtacaaaa tcctaaaaat ggagacattc ttgcgcttgc
    19141 cggaaagcag attaataaga gtggtaaaat gactgattat gacattggta cgtttacttc
    19201 tcaatttgcg gttggatctt ctgtaaaagg tggaacatta ttagccggtt atcagaataa
    19261 agctatcaaa gttggagaaa caatggtcga tgaaccatta catttccaag gtggtttgac
    19321 aaaacgatca tacttcaata aaaacgggca tgtaactatt aatgataagc aagctttgat
    19381 gcattcatca aacgtatata tgtttaaaac agcattaaaa ttagcgggag acccttatta
    19441 ttctggtatg gctttacctt cagacataag ttcacctgcc caaaagctaa gaagaggatt
    19501 aaatcaagta ggcttaggtg tgaaaacagg gatagattta ccaaatgaaa caagaggtca
    19561 aatcgaacca ttaacaaata atccaggtaa ttatctagat ttatcaattg gtcaatatga
    19621 tacctataca ccattacaat tatcacaata tgtttcaact atagcgaatg atggttatag
    19681 aatacagcca cacattggat taacgattca tgaatcaact aataaagatg aggttggtcc
    19741 actcaagaag aaaattaatg gcactgtctt gaacaaggtt aataatactg aaaaggaaat
    19801 caaacaaatt caagaaggat tcaaaatggc atttaatgat aaagatggta ctggatatgt
    19861 tagttttaaa gatacagtag tacctactgc tggtaaaacg ggtaccgcag aagtgttcca
    19921 aaacggagag ccaagagtta actctactta tataggatac gcgccaattg atgatccaaa
    19981 attagcgttt tcaattgtat atacaaatca gcctgtacca ccaccatggt taacaggtgg
    20041 agacttaggt agagatgtaa ttaactacta ctttaagcag ttaggtaaaa atgataaaaa
    20101 taaagacaaa gacaaataaa atttaacctg acgattgtgt agcgcatggt tgtaaaattt
    20161 taactttgca aatattatag atgttggtat aataataaag tcgtatttag aaatgataag
    20221 gaggaataac catgcgcgta aacgtaactt tagcttgtac ggaatgtggt gacagaaact
    20281 acattacaac taagaacaaa agaaataatc cagaacgtgt tgaaatgaag aaattctgtt
    20341 cacgtgaaaa caaacaaact ttacaccgtg aaacaaaata atcattaaaa ttaaatttaa
    20401 attaacaata aaaaaggtga aatctcaaaa taagaggttt caccttttta attatgcaca
    20461 cgtatgaaat gataaaagat tttcaattga aataataaat tttagaattg tcgcataatt
    20521 catcatgaca acataatgac atgtattgtt attaacgata taatagaatt gaataaagtg
    20581 ggtgataatg tgactaaaaa tgagattaga aaatacattt tacataaaat gaagaatttt
    20641 aataaagctg aaaagcgaaa agcagacaca tggttaagaa atcaattttt tgcaactgaa
    20701 gaatacaaag aagcaaacgc aattgcgcta gttctttctt ttaatcatga agtagatact
    20761 ttttctatta ttgaacaagc cttaatggat cataaacgta tttttgtacc gaaaatggat
    20821 tatttaaatc atcaaatgac ttttaaagag atatttaatc tcaaagatat tgatgtcgat
    20881 aataagggga tttactatcc aacttcaaaa ggtgaaacaa cgaataacct agatttaatt
    20941 gttgttcctg gtgttggatt tcaagacgat ggatatagaa ttgggtatgg tggtggctat
    21001 tacgacaggt ttttagctaa ttatcagaca aagacaataa gcttattata cgattttcaa
    21061 ataacatcat ttgaaccgga atcattcgat caaccagtcg ataaattgat tatatatcaa
    21121 gcagcatagt ggaggactaa atgaacatag acaaacaatt ttggaaaaca atatattatt
    21181 ggattaggta cttgaatttt gatattgtca gtagggaaaa agatgaccaa gaaatttggt
    21241 tagcccataa aagaaaaaaa caagtagtca tttttaaaca acatatcaag tctacccaag
    21301 aaatacgttt tgacaaagcg aaagtgcttg aacacaaaga tgaaatagca aattttattt
    21361 ctttcgaacc acaaagtttt gaattttatt attttacaga atcggaattt tcagaagaac
    21421 aattaaatga agtttcgcca attagaatta aattcaatgt tataagacac acaaaagatt
    21481 tgataaagca tatgccgaat atatttttgg ctagacttat ttcagaagat aatgataaaa
    21541 agacatatat gttctataaa cgcaaagtat taaccgataa ctttttagat aaatatatgc
    21601 agaaattttc accggcaaca tacacaataa tatttgtaaa tgtcttaata tggttatgta
    21661 tgattttata tttaaataat ttttcggatg taaaattatt agatgttggc gggttagtgc
    21721 attttaatgt cgtacatggt gaatggtatc gaattgttac atcgatgttt ttacatttta
    21781 gttttgaaca tatacttatg aatatgcttt cattatttat ttttggtaaa atagtcgaag
    21841 caattattgg ttcatggcgg atgttaactg tatactttat tgcagggttg tttggaaact
    21901 ttgtatcact atcatttaat acgactacaa tttcagttgg ggctagtggt gctatatttg
    21961 gtctgattgg atcaattttt gcgatgatgt atgtttcaaa aacatttaac aaaaaaatgt
    22021 taggacagtt attaattgca ttagtgatat tagttggtgt ttctctgttt atgtcaaata
    22081 taaatattgt ggcgcatatt ggaggattca ttggtggttt attaataact ttaattggct
    22141 attactataa agtgaatcgt aatatttttt ggattttact aattggtatg cttgttatat
    22201 ttattgcact tcaaattaga atttttacaa ttaaagaaga taatatttat aataaattga
    22261 tcaaagatga tatgactagt ggtaattatg ataatgctca aaatattgtg aagcagacaa
    22321 taaataaaaa ttatgccgat gatcaaacat attatttaag tggtatgatt atggctacta
    22381 tcaattctaa gtccgaaggt atgacagaat gggagcgagg acttagaatg tttcctaaat
    22441 caggtttatt aaattttgag ttagcgatag caaatcgttc attaaatgat gatgaaaaag
    22501 cattaaaata tgtgcgtaaa gcattaaatg cagaccctaa aaatacagat tatattaact
    22561 tagaaaaaga gttgactaaa tcaaatgagt cgaaaaataa ataactttta tgatgtacaa
    22621 cagttattga aaagttacgg atttctaata tattttaaaa atccagaaga tatgtacgaa
    22681 atgattcaac aggagatttc atcattgtat caatatgaac tgttatctaa ggaagaatat
    22741 ttgaaatgta cgttgataat taatcagaga aggaatgaac agaaatgaca aaaattattt
    22801 tagcagctga tgtaggcggg acgacttgta aattaggtat tttcacacct gaattagaac
    22861 aattacataa atggtctatt cacactgata catctgatag tacaggatat acacttttga
    22921 aaggaattta tgattcgttt gttgaaaaag taaatgaaaa taattataat ttttcaaatg
    22981 tacttggcgt aggtattggt gtaccaggtc ctgttgactt tgaaaaaggt acagtaaatg
    23041 gagcagtaaa cttatattgg ccagaaaaag ttaatgtacg tgagattttt gaacaattcg
    23101 ttgattgtcc agtgtatgta gataatgatg ctaacatagc tgctttaggg gagaaacaca
    23161 aaggtgctgg tgaaggtgcc gatgatgttg ttgccatcac acttggtaca ggtctaggtg
    23221 gaggaattat ttcaaatggt gaaatcgtac atggtcataa tggctctggc gcagaaatag
    23281 gtcattttag agcagacttc gatcaacgat ttaaatgtaa ttgtggtcgt tctggatgta
    23341 ttgaaacagt tgcttcagcg acaggcgttg ttaacttagt taacttctac tatccgaagt
    23401 tgacgtttag atcttctata ttagaattga ttaaagaaaa taaggttaca gcaaaagctg
    23461 tttttgatgc ggcaaaagct ggtgaccaat tctgtatttt cattactgaa aaggttgcaa
    23521 actatattgg atatttatgt agtattatta gtgttacaag taatccgaaa tatatcgttc
    23581 taggtggagg aatgtctact gcaggaccta ttttaattga aaatattaaa acagaatatc
    23641 ataatttaac atttgcacct gctcaatttg aaactgaaat tgtacaagcg aaattaggta
    23701 atgatgcagg tattacagga gcagcaggat taatcaagac ctatgtatta gataaagagg
    23761 gggtaaaata atggctattg ttgatgtggt tgttattcca gttggaacgg aaggtccgag
    23821 tgttagtaaa tatattgcag atattcagaa aaaacttcaa gaatataaag caatgggtaa
    23881 aattgatttt caattaacac caatgaatac tctaattgaa ggtgaattaa gcgatgtatt
    23941 agaagttgtg caagtgatac atgaattacc ttttgataaa ggtttaagta gagtttgtac
    24001 aaatatccgt attgatgacc gacgagacaa atctagaaaa atgaatgata aactaacatc
    24061 agtacaaaaa catttagaaa atagtggtga aaacctatga ggatttcaag cttaacttta
    24121 ggcttagttg atactaatac gtatttcatc gaaaatgaca aagctgttat tctgattgac
    24181 ccttcaggtg aaagtgaaaa aattattaaa aaattaaacc aaataaataa accgttaaaa
    24241 gctattttat taacacatgc acactttgat catatcggag cagtcgatga tatagttgat
    24301 cgattcgatg tcccggttta tatgcatgaa gcagagtttg attttctaaa agatcccgtt
    24361 aaaaatgggg cagataaatt taagcaatat ggattaccaa ttattacaag taaggtaact
    24421 cctgaaaagt taaacgaagg tagcacagaa atagaaggat ttaagtttaa tgtgttacac
    24481 acacctggac attcaccagg aagtttaaca tatgtgttcg atgaattcgc agttgttgga
    24541 gatacattat ttaataatgg aatcggacgt acagatttat ataaaggtga ttatgaaacg
    24601 ctagttgatt ctattcaaga taaaatattt gaattagaag gcgatttacc tttattccct
    24661 ggacatggtc catatacgac ggttgatgat gaacaattaa atccattttt acacggttaa
    24721 attgaagtaa taaaaacacc tcctacgtga ttatatgtag gaggtgtttt tttgaagatt
    24781 ctatttcaag aaataattaa taaagcgata gaaatgaaag cgagtgatgt acattttatt
    24841 ccagttaaaa atgaagtaag tattaaattt agaattaatg ataacttgga gcagtatgaa
    24901 caaattggga atagcattta tcaaaagtta ttagtttata tgaagtttca agctgggctt
    24961 gatgtttcta cacagcaagt cgcacagagc ggtcgatata gttacctttt caataaaata
    25021 tattttttga gaatatcaac tttaccattg tcacttggcc aagaaagttg tgttatcaga
    25081 attgtacctc aattttttca accacagaaa tcaacttata aattcaatga ttttaaacac
    25141 ctcatgaata agaaacaagg attactattg tttagtggac caactggttc aggaaagagt
    25201 acattaatgt atcaaatggt ctcatacgcg aataaagcct tgaatttaaa tgtaatttct
    25261 atagaggatc ctgtagagat gcaaattcct ggtatcgtcc aaattaatgt gaatgataaa
    25321 gctggcataa actatgtaaa ttcgtttaaa gctattttaa gatgtgatcc tgatgttatt
    25381 ttaataggtg aaatcagaga taaagatgtt gccaagtgtg ttatacaggc tagtttaagt
    25441 ggtcaccttg ttctgactac attgcatgca actgattgta aaggtgctat tttaaggcta
    25501 ttagaaatgg gcatttctgt acaagaattg atacaggcaa ctaacttaat tataaaccaa
    25561 cgacttgtaa ctactattaa gcaacagcga caattagtat gtgaaattct atctcagcaa
    25621 caactccgat atttcttttc ccataatcat tcattaccat catcatttaa gaacttagaa
    25681 gataaacttg atgatatgac aaaagcaggt gtcatttgtg aaactacaat gcataaatac
    25741 atttaaacta cattctaaga agcgacaatt aagtaaggcc caacaaatcg acttactttc
    25801 aaatttatgt aatttgttga aatatggttt cactctgtat caaagttttc aatttttaaa
    25861 tcttcaaatg acatataaaa ataagcaatt aggtaccacc attctaagtg aaatttcaaa
    25921 tggtgcacca tgcaatcaaa tattatcact gataggttat agcgatacta tcgtcatgca
    25981 agtatatttg gcagaaagat ttggcaatat tatagacgtt ctagaagaaa ccgtaaatta
    26041 tatgaaagtg aatagaaagt cagaacaacg attgttaaag acactgcaat accccttaat
    26101 actagtttct atctttattg ctatgattat tatattaaac ctcacagtaa ttccacagtt
    26161 tcaacaatta tatacttcta tgaatattca actatcttct tttcaaaaaa cattgtcttt
    26221 tttcattacc agcttaccta ctataattgt agtaatgctc ataatagtat ctatgttggc
    26281 tattattatg aaattaattt ataacaattt aaatatgctc aataagataa actttgtgat
    26341 gaaactaccg ctaatatcag gctatttcca attatttaaa acttattttg taactaatga
    26401 attagtgttg ttttataaaa atggtattac acttcaatca atagtagacg tttatattaa
    26461 ccatagtagt gatccattta gacagtttct aggtaaatac ttattaactt attcagaaat
    26521 gggatatggt ttgcctcaaa ttttagaaaa actaaaatgc tttaagcctc aattaattaa
    26581 gtttgtgcta caaggtgaaa agagagggaa gctagaagta gaactaaagt tatattcgca
    26641 aatattagta aaacaaatag aagataaagc gataaaacag actcagtttt tacagcctat
    26701 tttatttttg attttaggtt tatttattgt cgcaatttat ttagtaatta tgttaccaat
    26761 gtttcaaatg atgcaaagta taaaataatg gaggttaata tatgtttaaa tttcttaaga
    26821 aaactcaagc gtttacattg atagagatgc tattagtgtt attaatcatc agtttattat
    26881 taattttaat cattccaaat attgctaaac aaactgctca catacaatca acaggttgta
    26941 atgcacaggt aaaaatggtt aatagtcaaa ttgaagcgta tgcattgaaa cataatagaa
    27001 atccatcgtc tattgaagac ttaattgcag atggttttat aaaagaagca caaaagacat
    27061 gtaaatcagg agagacaatc acaattagta atggagaagc agttgcaaat tagaaagcag
    27121 tcagcattta ctatgattga gatgcttgtg gtaatgatgt taatcagtat atttctactt
    27181 ttgacaatga catctaaagg attaagcaat cttagagtaa tagatgatga ggcaaatatc
    27241 atttctttta ttactgaatt gaattatatt aagtcgcaag ctatagcaaa tcaaggatat
    27301 atcaatgtta gattttatga aaacagtgac actattaaag taatagagaa taataatata
    27361 cgatttctaa aattaaaagt aggcaaaata attaatgttg caaaagttga tattattgcc
    27421 tttgataaaa aagggaatat caataaattt ggtagcataa caatttacaa taacaattca
    27481 atttatagaa taatattcca tattgaaaaa ggaagaattc gttatgaaaa gctataagtg
    27541 taaaggttca ttcttaatag atagtatggc tggatttttg ctaattggat tgattacatt
    27601 actattgata ccaatgatga atcaaatgca agcgagtata aaccataaac tacaaacaat
    27661 tgatgcttct aaagtaattt tgacgactgt atctaaaatt aataaagaag aacttaagaa
    27721 gggggtaact atagggaagt atgatattaa gcaaagtgac caacaaattt gtgctatttc
    27781 aaaaaatacc acttcttatc aaaagacatg tatacagtat taatgtcaaa gctttttcgc
    27841 tcattgaaat gttagtagcg atgatggtta taagtataac tttactaatt gttccagact
    27901 taattagact tagtaaaact tttctaattg aaagtaggga tttaacaact gtagatttcg
    27961 aatttttctc aagagatatt ctagatgatt ttaaaggagt agatagaaac gatattgaaa
    28021 ttaggcaaca ccgtatcatt ttacataaag gtgaagaaat gatcgaatac aaattaatta
    28081 ataataaaat tattaaagtt gtaaatgaca gaggaaatat aacaatgatt aataatgtta
    28141 cagcatttac tgcaaatatc tactataaat ccattattaa aataacgata acagttaaag
    28201 tcggtacaaa tgtgcagact aaaactattt atgtataact tactagtatt taaatgagta
    28261 ctattaaaaa ggatgtgaaa taatttatta attttgattt atataaagaa aaaggatatg
    28321 ttacgccgtt cttattgatg ctaacagcaa tttatttttc cattttcact ttttatatca
    28381 gtcaatatag tttaaaatta aagacattat ataatattga aactgactat aactataaga
    28441 ttgttttgtt attgaaagaa gggaaaacgc atgaatcatg ataaatcacc aataatttta
    28501 attggtttca tgggtaccgg taaatctacg attggtaaat acgttgcaga tgagcaaaat
    28561 ttatcattta ttgatataga ttcatatatc gaagagaagt ataagttaac aataccagaa
    28621 atattttgta aacatggtga acaatatttc aggaatttag agttcacatg tttgcaagaa
    28681 tgtattaaca ctgcagatat aattgctact ggtggtggta ttattgagag tgaagaggca
    28741 tttaattttt tgaaaaatca aaaaaacatt atttggttag attgtaatat tgatattata
    28801 tatagtcgaa tcaatgatga cccacatcga cctaatgcaa ataataagac aatcaagcag
    28861 ttaaatgact tgtattgctc gcggaattta agatataatg aaatcgcatt caagaaattt
    28921 gatagtcatt tgctatcaat ttcagaaata tattatgaat tgctaaattt aataaaagcg
    28981 agtgatcagt attagagaga atagagcgtt aagactctat cgccgaaggt gcaagtaatt
    29041 tattacgaaa ctctcaggca aaaggataat actgtaacgc gttcctgaat tggtgattta
    29101 taaacagggt agcgattgct atcctgtttt tataatttta agggggtatt tcaatgtcaa
    29161 gtgatttaaa acaaacacct ttatatcaaa attatgttga tagaggtgca aaaattgtgg
    29221 aattcggagg atgggcgatg cctgttcaat tttcaagtat taaagaggag cataatgctg
    29281 ttcgatacga aattggcctg tttgatgtta gtcatatggg tgaaattgaa gtaacaggta
    29341 aagatgctag tcagtttgtg caatatttat tatcaaatga tactgataat ttaactactt
    29401 caaaagcatt atatactgct ttatgtaatg aagaaggcgg tattattgat gatttagtaa
    29461 tatataaatt agctgacgac aattatttat tagttgttaa tgctgctaat actgaaaaag
    29521 attttaattg gattttaaaa cacaaagaga aatttgatgt tgaagtacaa aatgtatcaa
    29581 accaatatgg tcaattagca atacaaggac caaaagctag agatttaatt aatcaattag
    29641 ttgatgaaga tgtaactgaa atgaaaatgt ttgaatttaa acagggtgtc aaattatttg
    29701 gagcaaacgt cattttatct cagtcaggtt acacaggtga agatgggttt gaaatttatt
    29761 gtaatatcga tgatactgaa aaaatttggg atggtttatt agagtataat gttatgccat
    29821 gtgggttagg cgctcgtgat acattaagat tagaggctgg attgccatta catggacaag
    29881 atttaactga atcaattaca ccatatgaag gtggtatcgc ttttgcaagt aaaccattaa
    29941 ttgatgctga ttttattggt aaatctgtat taaaagatca aaaagaaaat ggtgcaccaa
    30001 gaagaacagt gggattagaa ttacttgaaa aaggaattgc aagaactggt tatgaagtta
    30061 tggatttaga tggaaatatt attggagaag taacttcagg aacacagtct ccatcatcag
    30121 gaaaatcaat tgcacttgca atgataaaaa gagatgagtt tgaaatgggt agagagttgc
    30181 ttgttcaagt tcgtaagcgt caattaaaag cgaaaattgt taagaaaaat caaattgata
    30241 aataattaaa aaggggtgtg cattgtgagt catcgttata tacctttaac tgaaaaagac
    30301 aagcaagaaa tgttacaaac aattggtgca aaatctatag gagaattatt cggtgatgta
    30361 ccaagtgaca ttttattaaa tagagattta aatattgctg aaggcgaagc ggaaacaacg
    30421 ttacttagaa gattaaatcg cattgcaagc aagaatatca ctaaagaaac gcatacatca
    30481 tttttgggtg ctggtgttta tgatcattac gcgccatcag ttgtggatgc tatgatttca
    30541 agatcagaat tttacacagc atatacgcca tatcaaccgg aaatttctca aggtgaatta
    30601 caagccattt ttgagttcca aactttaatt tgtgaattaa cagatatgga tgtagcaaat
    30661 tcttctatgt atgatgggat gactagcttt gctgaagcat gtatcttagc atttagtcaa
    30721 acaaagaaaa ataaaattgt agtctcaaaa ggtttacatt atcaagcatt acaagtactt
    30781 catacttatg ctaagacgcg taaggaattc gaagtagtgg aaatcgattt agatggcact
    30841 gttacagatt tgaaaaaatt agaagcagca gtggatgatg aaacagcagc tgttgcagta
    30901 caatatccaa acttttacgg ttcaattgaa gatcttgaaa agattcatag ctttattgaa
    30961 gataaaaaag cattatttat cgtatatgca aatccattag cattgggatt acttacacct
    31021 ccaggttcat ttggtgcaga tattgttgta ggtgatactc agccattcgg aattcctgct
    31081 caattcggtg ggccacattg tggatatttt gcaacgacga aaaagttaat gcgtaaagtg
    31141 ccaggtagat tagtcggtca aacacaagat gatgaaggta atcgtggctt tgtattaaca
    31201 ttacaagcac gtgaacaaca tattcgacgc gataaagcga catctaatat ttgttctaac
    31261 caagcattaa atgctttagc gtcatcaatt gcaatgtcag cacttggtaa acaaggtatc
    31321 tatgatattg cagttcaaaa tatagaacat gctaactatg ctaaacaaca atttattaaa
    31381 aaaggttttg aagtattaga tggtacatct ttcaatgagt ttgtagtcaa atttgacaaa
    31441 ccaattcaac aagttaacga ggaattagtg aagtacaata ttatcggtgg ttttgattta
    31501 ggtgttgttt cagacgactt taaaaatcac atgttaatag ctgtaactga attaagaaca
    31561 aaagatgaaa tcgatacatt tgtagaaaag gctggtgagt taaatgacta gtaaatcaag
    31621 tccattaatt tttgaaagat ctcgtgaagg cagatatgca tattcattac caaaaagtga
    31681 tattaaaaca aattctgttg agtcattgtt agatgataaa tttattcgta aaaacaaagc
    31741 ggagtttcct gaagttgctg aactagactt agtacgtcat tatactgaac tttcaaataa
    31801 aaatttcggt gtagacaacg gattttatcc attgggttca tgtacgatga agtataaccc
    31861 taaaattaat gaaaaggttg ctagaatacc aggatttagt gaatcacatc cattacaaga
    31921 tgaagatcaa gttcaaggtt cattagaaat tatttatagt ttgcaagaag aattaaaaga
    31981 aattactggt atggatgagg tgacattaca accagctgct ggcgcacatg gtgaatggac
    32041 tgcattgatg atatttaaag cttaccatga gaataatggt gaaggtcatc gtgatgaagt
    32101 cattgtgcca gattctgcgc atggtacgaa tccagcctca gcttcatttg caggatttaa
    32161 atcagttact gtaaaatcaa acgaacgtgg cgaagttgat attgatgact tgaaacgtgt
    32221 tgtaaatgaa aatacagcag ctattatgtt aactaatcca aacactttag gtattttcga
    32281 aaaaaatatt atggaaatcc gtgaaatcgt ccataatgct ggtggtctat tatattatga
    32341 tggtgcgaat ttaaacgcta ttatggacaa agttcgccca ggagatatgg gatttgatgc
    32401 tgttcattta aacttgcata aaacatttac tggtccacat ggtggtggcg gtcctggttc
    32461 aggtccagtc ggtgtagtaa aagaactagc aagttactta ccaaagccaa tggttattaa
    32521 agatggcgac aaatttaaat atgataatga cattaaaaat tctatcggac gtgtaaaacc
    32581 attttatggt aactttggta tttacttaag agcttatacg tatattcgaa ctatgggagc
    32641 aactggactt aaagaggttt ctgaagcagc ggttcttaat gcgaattata ttaaagcacg
    32701 tttatctaaa cactttgaaa taccttataa acaatattgt aaacacgagt ttgtgttaag
    32761 tggtgtgcgt caaaaagaat ttggtgtacg tactttagac atggctaagc gattattaga
    32821 tttcggtgta catccaccaa caatatactt cccattaaat gttgaagaag gtatgatgat
    32881 tgaaccgact gagacagagt ctaaagaaac acttgattat tttatcgata cattaattag
    32941 tattgctgaa gaagctaaaa atgatcctga taaagtgcta gaagcaccac atacaactgt
    33001 gattgatcga ttagacgaag ctacagctgc tcgtaaacca atattaaagt ttgaaaatct
    33061 taaacaggaa aaataaagta ttaaacacat attccgagaa ttattattct aactttgtat
    33121 gaagatttaa ggataatggt ttcaaaatca attgaaaaag acaatttcta tttaaacaag
    33181 aaaactaaac cgaagtaata actcttaggg tttggtatta ttctttcata gaaattgtct
    33241 tttcattttt tagattgcgg taattgaatc gtattgaaaa tgagctgaac tttctttatt
    33301 atgctgaaac taagtttaat gatcagtgaa gctagagaaa tatagtaaca gactttgtta
    33361 aaaaattaaa atctgagtat tgttattgat gatcaattaa cactcagatt ttattaaatc
    33421 gtatattaaa tttacaaaaa ctatttttta gactttattt ttccagtcca ttttttatag
    33481 ccgcctttta acatatagat atctgtatat ccattctttt tcaaaatacg agcggctcta
    33541 tagctagcaa tcccattggc atcacataag tataccggtt gatcttttct taatccttgg
    33601 aatcgttgcc tgaacattgt cataggaata ttgcgagacc cattaatgtg accgtagtca
    33661 tagtcaactt tctctctaac atcgatgact tgagcttttc taatcccatt atggaattca
    33721 ttttgatcta attctttaac agctcgcttg ttaagaattt gttgaacaat catataagca
    33781 ataattgcta taactaaaat tattgcgatg tacaaactag cactcatctt gcatcctccc
    33841 taattaaacg ataatataat tataagactg ttaggtcaaa ttatcaaaat atttcactaa
    33901 agaattgcaa ttattattca tttagttaaa ttgattaatt agcgaatcaa atattataca
    33961 ttttgctgat aggagttctt gaatatgact gaaacttgga attttattaa tacaggaagt
    34021 aaagacccat attataatat ggcaatggat gaagcgttat taaattttgt ctctagaggt
    34081 gaaattgatc cagttatacg tttttacaca tggaatcctg caacattatc aatagggtat
    34141 ttccaaaggc tacaaaaaga aattgatatt gataaggtta aagagaaagg ttttggtctc
    34201 gtaaggcgtc aaactggtgg ccgtggggtg ctacatgata aagagttaac ttacagtgtg
    34261 attgtgcctg aatcgcatcc gaatatgcct tctacagtta cagaagcgta cagagtgatt
    34321 tcacaaggat tattagaagg atttaaaaac ttaggatttg atacttattt tgcggtacct
    34381 aaaacgcctg aagagcgtca aaaattaaaa caacctcgaa gttcagtgtg ttttgatgca
    34441 ccaagttggt atgagctagt tgttgaaggc cgtaagattg ctgggagtgc tcaaacgaga
    34501 caaaagggcg taatcctcca acacggttcg attttacaag atattgatat tgatgaactg
    34561 tttgatatgt ttatttataa aaatgaaaga ttaaaattaa aaatgaaaga ggcattcgtt
    34621 gaaaaagcag ttgccatcaa tgatatttca gatgaacaca ttactattag tcaaatggaa
    34681 gaagctttcg agaaaggttt taagaaaggt ttaaatatag aatttaagcc acttgaatta
    34741 acagaagctc aattggcaga agtggaagaa ttaactgaaa aatatcgttc agatgaatgg
    34801 atgtttagaa agtagttttt agaataaaaa atggaagcac gtagactatt ttacctacaa
    34861 gtgcttccgt ttttttatct attttcttct ttgatttctt ttatacttac gcattgcttt
    34921 gcgatatttg cgttggtctt cagttaagat gaagaaataa taaatcagat aaataatacc
    34981 agctattaca gcaaagctga ttaacattct gacgattgaa aaaataaagg catctagatt
    35041 cattactaat ccaatcgcag caataataag tacaagataa aatattatat tacgcattaa
    35101 aagactccta tcatgatttt gcatttaagt ttaatttcga caacgcttgt tgtccttttg
    35161 taagttgttt tttatcttta ttttgatagc catttttaat atcatcaaat gcatgattca
    35221 aatcagagtt taatttatcg atatttttag acgttttttt atcatcatca ctaagtgata
    35281 aaccatcgat agcatctttg tatgaagtgt gagcactttg aatatcatta gaaatttgat
    35341 ttaatttcga ttgaacattc ttatcgcctt tgtttttact tacatctttc tcaatatcgt
    35401 tataggattt caaagcttta gcaactttgt cgtagtatgt tgatgtaaca ttcatcaatt
    35461 taattttcgt attattagat gcagtttttt tactgttttc tttatccttt tcaagccctt
    35521 taattttaga tttagaatca ctaatttgtt gattaagttc ttgaatatct agtttgagtt
    35581 gatgattatc ttctcttaaa tctgttgttt tttcttctaa tggtgctaaa ttttgtgatc
    35641 cacatccagc taacaataat gttgcgccaa caattgaaac caattttttc attactatct
    35701 ccctaaaatc aatatctatt attttttatc cttattatga tattatttta atgtataaag
    35761 ttgaaatgca caaacgtttc actaggaggt aataagatga gcagaataac acaagtgcat
    35821 cgtattttag aacaaaaaca tttagatgcg attatcattc tttcggatta caatagaaga
    35881 tatttatcag gttttactgg taccagtgga gcacttatca tatctaaaga taaacaatat
    35941 ttgataacag actttagata tattgatcaa gcaactaagc aggcgccaaa ttatgaaatt
    36001 attaatcgta aatctactat tattggtgag attaaagaat tgctacacca agaaaatttt
    36061 gaaaatgtag gttttgaggg gcatcatgta agttatgata cataccttga attaaataaa
    36121 agccgtatat cattaataag catttctaat actgtagata aaattagaga cgtcaaagat
    36181 gctgacgaaa ttgctttaat tcaaaaagca gctaatattg ttgatgaaac atatgaatat
    36241 attttaactg ttgtaaaagc aggcatgact gaaaaagaat taaaggcaat attagaaagc
    36301 aaaatgctag aattaggagc agatggacca tcattcgata cgattgtagc atctggtcat
    36361 agaggtgcat taccacatgg tgttgcaagt gataaaatta ttgaaaaagg cgacatgatt
    36421 acattagatt ttggcgcgta ttataacggc tattgttcag atattactag aacatttgct
    36481 attggagaac cagatcctaa actgaaagaa atatatcaaa tagtacttga atctcaaatg
    36541 aaagcaatta atgagattag acctggcatg actggtgcag aagctgatgc catttcaaga
    36601 aactatttag agtcaaaagg gtatggaaaa gaatttggac attcactagg acatggtatt
    36661 ggtttagaaa tccatgaagg gccaatgctg gctcgtacga tacaagataa acttcaagtt
    36721 aacaactgtg ttacagtaga acctggtgtt tatatagaag gtttgggcgg tataagaata
    36781 gaagatgata ttttaattac agaaaatggt tgtcaagtct ttactaaatg cacaaaagac
    36841 cttatagttt taacataagc gtgtaaaatg aggaggaaac tgaatgattt cggttaatga
    36901 ttttaaaaca ggtttaacaa tttctgttga taacgctatt tggaaagtta tagacttcca
    36961 acatgtaaag cctggtaaag gttcagcatt cgttcgttca aaattacgta atttaagaac
    37021 tggtgcaatt caagagaaaa cgtttagagc tggtgaaaaa gttgaaccag caatgattga
    37081 aaatcgtcgc atgcaatatt tatatgctga cggagataat catgtattta tggataatga
    37141 aagctttgaa caaacagaac tttcaagtga ttacttaaaa gaagaattga attacttaaa
    37201 agaaggtatg gaagtacaaa ttcaaacata cgaaggtgaa actatcggtg ttgaattacc
    37261 taaaactgtt gaattaacag taactgaaac agaacctggt attaaaggtg atactgcaac
    37321 tggtgccact aaatcggcaa ctgttgaaac tggttataca ttaaatgtac ctttatttgt
    37381 aaacgaaggt gacgttttaa ttatcaacac tggtgatgga agctacattt caagaggata
    37441 atctctaatt tgttaacaaa tagcttgtat tcactatact gatttaacgt aagatattct
    37501 aaataagtct cataaagcta ttgcctaaaa tgattatagg ttatatgctg atatgaggct
    37561 ttttattttt aaaataattt ttaaaatata aaacgaatcg tctaattggt agtgaaaaaa
    37621 catagacatg agtagaggta tatatctaca tatcatattg ttgaggataa aacataatag
    37681 ctaataaaaa taacattgat atgtttaact gaatgcataa aatttttaat caagagttaa
    37741 gatgagtcaa tgttaagtaa taaatacaca agtgttttta atttaaaatg tggttttatt
    37801 ataactttgg gatagactta taatgtcgcc agcaatatga acatgcttga attgaagagt
    37861 tgtctcaagt aaaatagacg ggtagatgaa aacaaactga aggagtcagt aataatgaac
    37921 tttaaagaaa tcaaagaatt aattgaaatt ctggataaat caactttaac ggaaatcaat
    37981 attgaagata ctaaaggcaa agtgacgctt aagaaagaaa aagaaactga gattatcacg
    38041 ccacaaatct cacaaatgcc agttgaagct gcggcaatgc ctatgcctca agcacaatca
    38101 actgatagca ataaaactga agctccaaag ccaacttcag ataatcacaa aacaattaat
    38161 gcacctatgg taggtacatt ttacaaatcg ccatctccag acgaagaagc atatgtgcaa
    38221 gttggggaca ctgtttcaaa tgaaacaaca gtgtgtattt tagaggcaat gaaactattt
    38281 aatgaaattc aagcagaaat ttcaggtgaa attgttgaaa tcttagtaga agacggacaa
    38341 atggtagagt atggccaacc gttatttaag gtgaaataat gaaaaaggtt ttaattgcaa
    38401 accgcggtga aatcgcagtt aggattattc gcgcttgtcg tgatttaggc atccaaactg
    38461 ttgcaatcta ttctgaaggg gataaagatg cgctacatac tcaaattgct gatgaagcat
    38521 attgcgtagg tcccactttg tctaaagatt catatttaaa tattccgaac atcttatcta
    38581 ttgcaacttc tacaggttgt gatggcgttc atccgggtta tggcttttta gctgaaaatg
    38641 ctgattttgc agaattatgc gaagcatgcc aattgaagtt cattggacca agttatcaat
    38701 ctatccaaaa aatgggtatc aaagatgttg ctaaggcaga aatgatcaaa gccaatgttc
    38761 cagttgttcc tggtagtgac ggtttaatga aagacgtctc agaagctaag aaaatcgcca
    38821 aaaaaattgg ctatccggtc atcattaaag ctactgctgg cggtggcgga aaaggtatcc
    38881 gtgttgctcg tgatgaaaaa gaacttgaaa ctggcttccg aatgacagaa caagaagctc
    38941 aaactgcatt tggtaatggt ggactttata tggagaaatt catcgaaaac ttccgccata
    39001 ttgaaatcca aattgttggg gacagctatg gtaatgtaat tcatttagga gaacgtgatt
    39061 gtacaattca aagacgtatg cagaaattag tggaagaagc accttcccca attttagatg
    39121 atgaaacacg tcgtgaaatg ggaaatgccg cagttcgtgc agcgaaagct gtaaattatg
    39181 aaaatgcggg aacaattgag tttatatatg atttaaatga taataaattt tattttatgg
    39241 aaatgaatac acgtattcaa gtagaacatc ctgtaactga aatggtaaca ggaattgatt
    39301 tagttaaatt acaattacaa gttgctatgg gtgacgtgtt accgtataaa caagaagata
    39361 ttaaattaac aggacacgca attgaattta gaattaatgc tgaaaatcct tacaagaact
    39421 ttatgccatc accaggtaaa attgagcaat atcttgcacc aggtggatat ggtgttcgaa
    39481 tagagtcagc atgttatact aattatacga taccgccata ttatgattcg atggtagcga
    39541 aattaatcat acatgaaccg acacgagatg aagcgattat ggctggcatt cgtgcactaa
    39601 gtgaatttgt ggttcttggt attgatacaa ctattccatt ccatattaaa ttattgaata
    39661 acgatatatt tagaagcggt aaatttaata caaacttttt agagcaaaat agcattatga
    39721 atgatgaagg ttaataggag gtcaatccat ggtcaaagta actgattatt caaattcaaa
    39781 attaggtaaa gtagaaatag cgccagaagt gctatctgtt attgcaagta tagctacttc
    39841 ggaagtcgaa ggcatcactg gccattttgc tgaattaaaa gaaacaaatt tagaaaaagt
    39901 tagtcgtaaa aatttaagcc gtgatttaaa aatcgagagt aaagaagatg gcatatatat
    39961 agatgtatat tgtgcattaa aacatggtgt taatatttca aaaactgcaa acaaaattca
    40021 aacgtcaatt tttaattcaa tttctaatat gacagcgata gaacctaagc aaattaatat
    40081 tcacattaca caaatcgtta ttgaaaagta atgtcatacc taattcagta attaaataaa
    40141 gaaaaataca aacgtttgaa ggagttaaaa atgagtcgta aagaatcccg agtgcaagct
    40201 tttcaaactt tatttcaatt agaaatgaag gacagtgatt taacgataaa tgaagcgata
    40261 agctttatta aagacgataa tccagattta gacttcgaat ttattcattg gctagtttct
    40321 ggcgttaaag gtcacgaacc tgtattagac gagacaatta gtccttattt aaaagattgg
    40381 actattgcac gtttattaaa aacggatcgt attattttaa gaatggcaac atatgaaata
    40441 ttacacagtg atacacctgc taaagtcgta atgaatgaag cagttgaatt aacaaaacaa
    40501 ttcagtgatg atgatcatta taaatttata aatggtgtat tgagtaatat aaaaaaataa
    40561 aattgagtga tgttatatgt cagattattt aagtgtttca gctttaacga aatatattaa
    40621 atataaattt gatcaagatc cacatctcca atctgtattg attaaaggtg agctttcaaa
    40681 tttcaaaaag cattcaagtg gtcatttata ctttaatgtg aaagataaag aaagcgttat
    40741 aagtgccatg atgttcaaag gtagtgcttc taaattaaac ttcgaaccta aagaaggaga
    40801 tgaagtctta ttagaagcac gtgtttctgt ttttgaacgt cgtggaaact atcaaattta
    40861 tgtaaataaa atgcaattag atggtatagg gaacttatat caaaaattag aagcattaaa
    40921 gaaaaaatta actgaagaag gttgttttga taaagcgaat aagaaatcaa tacctaagtt
    40981 tcctaaaaaa attgcagttt taacagcgag tacaggtgcc gcaattcgag atatccattc
    41041 aacgataaat agtcgttttc cattagctga acaaatacaa attagtactt tggttcaagg
    41101 tgaaaaagca aaagacgaca ttattgaaaa aattgaatat gcagatagtt taggtgtaga
    41161 taccattatt gtaggtcgag gcggtggatc catagaagat ttatggaatt tcaacgaaga
    41221 agctgtcgtc cgtgcaatat ataattgtaa gacaccgatt atatcagcag ttggtcatga
    41281 aacagacttt acattaagtg attttgctgc agacatcaga gctgcgactc caactcaagc
    41341 tgctgttatt gcaacaccag accaatatga attgctgcaa caaatacagc aatatcagtt
    41401 tacattgact cgtttcatca agaaacattt agaacaacaa cgtaaacatg ttgaacattt
    41461 gtcatcgtat tacaagttta aacaaccaac tttattgtat gatcaacaga tacaacgtcg
    41521 agatgattta gaaaagagac tgaaacaaca aattcaggca acttttgaac aacagagaca
    41581 tcgattaatg ctgttgcaac aacgttataa tttaaaagca ttactaagta gtgttaatca
    41641 agaacaacaa aataatcttc aattgacaaa ccaacttgtt aaattattga attctaagat
    41701 attaagttat aaaaatgatt tgaaaaataa agtagaaaac ttaaacaatt taagtccaac
    41761 taatacaatg ttgcgtggat atgcaattgt taataaaaaa gacgaagtca ttacgagtac
    41821 taaagattta acagaaaatg atcaattgac attaacaatg aaagatggct tagtagatgc
    41881 aaaagttacg aaagtaaggt gtaataatga ctaaagaaac gcaaagtttt gaagaaatga
    41941 tgcaagaatt agagcaaatt gttcaaaaat tagataatga aacagtatct ttagaggaat
    42001 cattagattt atatcaacgt ggtatgaaac tatcagcagc ttgtgacaca actttaaaaa
    42061 atgccgaaaa aaaggtgaat gacttaataa aagaagaagc tgaggatgta aaaaatgacg
    42121 aatctaccga tgaataaatt aatagatgaa gtcaataatg aattatcggt tgcgataaat
    42181 aaatcagtaa tggatactca gctagaagaa agtatgttgt attcattaaa tgctggaggt
    42241 aaacgcatcc gaccagttct gttattactc actttagatt cactaaatac cgagtatgag
    42301 ttaggtatga agagcgcaat tgcactagaa atgattcata catattcact tattcatgat
    42361 gacctaccag cgatggataa tgatgattat cgacgaggaa aattaacaaa tcataaagta
    42421 tatggtgagt ggactgcgat attagcaggt gatgctttat taactaaagc atttgaactt
    42481 atttcaagtg atgatagatt aactgatgaa gtaaaaataa aagttctaca acggctgtca
    42541 atagcaagtg gtcatgttgg aatggtcggc ggtcaaatgt tagatatgca aagcgaaggc
    42601 caaccaattg atcttgaaac tttggaaatg atacacaaaa caaaaacagg agcattatta
    42661 acttttgcgg ttatgagtgc agcagatatc gctaatgtcg atgatacaac taaagaacat
    42721 ttagaaagtt atagttatca tttaggtatg atgttccaga ttaaagatga tttattagac
    42781 tgctatggtg atgaagcaaa gttaggtaaa aaagtgggca gcgatcttga aaataataaa
    42841 agtacgtacg tgagtttatt agggaaagat ggcgcagaag ataaattgac ttatcataga
    42901 gacgcagcag tggatgaact aacgcaaatt gatgaacaat tcaatacaaa acacttatta
    42961 gaaatcgttg atttatttta tagcagggat cactaaaagc aaatatatcg attagcaaca
    43021 attgtaaagt ttgaaatgtc tcaaaaggca tattcaactg acgttgtttg ttaatcgatt
    43081 tttctcatgt tgaaacttaa aattatgttg aagactttca tttaaaaaat agaattttca
    43141 atttctatac gacaatattc gtatctgtga tatcaatgtg attttaatta aatatgttca
    43201 aacaattgac gcttattaaa tctaataatt agcaagttta ttacacatat attcattttt
    43261 atacatattt taagcaattg ttatgcgtaa tatgaaaaat atttataaat agataaccaa
    43321 gatagataaa ccatgtttac cctttttacg taaaggtgtt tagtagtcgt aaatcagtgc
    43381 atgtgttaaa ataaatgtat aaatattcgt aatcagaggt gtttactgtg cccaaaaaat
    43441 cggttaggca tataaaaatt agagaaatta tttcaaatga acagatagag acacaagatg
    43501 aattagttaa acgattaaac gattatgatt taaatgtcac tcaagcaact gtttctcgtg
    43561 atattaaaga actacaactt attaaagtac ctataccttc aggtcaatat gtttatagtt
    43621 taccaaatga tagaaaattc catcctttag aaaaattggg acgttattta atggattcct
    43681 ttgttaatat agatggtact gataatttac ttgttctaaa aacattacct ggtaatgcac
    43741 aatctattgg agctatatta gaccaaatca attgggaaga agtactaggc acaatttgtg
    43801 gtgatgatac ttgtttaatt atttgtcgaa gcaaagaggc aagtgatgaa atcaagtcaa
    43861 gaattttcaa tttgttataa ggatgcgatg agtttatgtt acaaacctta tcaatcaagc
    43921 aatttgctat tattgaagaa ttagaaattc aattttctga tggtttaact gttttaagtg
    43981 gtgaaacggg ttcaggtaaa tcaattatta tagatgcaat tggacaatta attggaatga
    44041 gagcttcatc tgattttgta agacatggtg aaaaaaaggc tgtcatcgaa ggtatatttg
    44101 atattgacga gagtaaagat gctatacata tattgaaaaa tatggatatt gatgtagatg
    44161 aagatttttt attagttaaa cgggaaattt tcagctctgg taaaagtctt tgtaaaatta
    44221 ataatcaaac agttacttta caagatttac gaaaagttat gcaagaatta ttagatattc
    44281 atggccaaca tgaaacgcag tctttgttga aacaaaaata ccatttaaca ttattggata
    44341 attacgcaga atcacgttat caagatttgt tggataaata tcaccaaact tttcaaaatt
    44401 ataaagccaa aaagcaagag ttagaagatt tagaatcagc agaccaagca ttgctacagc
    44461 gtttagattt aatgaaattc caattagaag aattgtctga agcacatcta aaagaaggcg
    44521 aaatagagca acttgaaata gatattaaac gcattcaaaa ctctgaaaaa ttaagcttag
    44581 cgcttaataa cgcacatatg acattaacgg atgaaaatgc tatcactgat cgtttatatg
    44641 aactaagcaa ccatttgtta acaataaacg atatagttcc aaacaaatat gacaaattaa
    44701 aagaagatat tgatcaattt tactacattt tagaagatgc aaaacatgag ttatatgatg
    44761 aaatggctaa tacagaattc gacgaacaag tgttaaacga gtatgaatct cgtatgaatt
    44821 tgctgaataa tttaaaacgt aaatatggaa aagatatttc agaattaatc gcatatcaag
    44881 aaaagcttaa taatgaaatc aataaaattg aaaactatga acaaagtaca tcgcagttac
    44941 gagaagaaat aaatgcattg tataatcaag ttatagaggt tggacaagcg ttatcaaagc
    45001 agcgtcgtat agtcgctagg gagttaagag accacattgt atcggaaatt caaaacttac
    45061 aaatgaaaga cgcaaatctt gagatttcat ttaaaaaatt agaagaaccg aatattgatg
    45121 gaatcgaatt tgtagaattt ttaatcagtc caaataaagg ggaaccatta aaaagtttaa
    45181 ataaaattgc gtcaggtgga gaactttcta gaattatgtt agcgttaaaa agtatttttg
    45241 ttaaatcgag aggtcaaact gcaattttat tcgatgaggt tgactcaggt gtatctggac
    45301 aagctgcaca aaaaatggca gagaaaatgc gagatatagc agaatatata caagttattt
    45361 gtatctctca cttaccacaa gttgcatcga tgagcgacca tcatttatta atttcgaaat
    45421 catcaaaaga tgatcgtaca acaacacaag ttcaagagtt aattagcgat gataaagtag
    45481 atgaaattgc acgaatgatt tcaggtgcaa gtgttactga tttaactcga gaaaatgcaa
    45541 gagaaatgat acaacacaat caaagacgta gataatttcc aaagtgtgtt gaatactcat
    45601 ttatggattt atttatgtgc attcaaacta aatagacaat catgtaaaat aggtaattaa
    45661 tggacatgaa aactatgtat taataaattt tcaatggata tatatcatta gttaggagta
    45721 agaacatgtc agagaaacaa tatgatttag tcgttctcgg tggaggtact gcaggatatg
    45781 ttgctgcgat acgtgcgtct caattaggta aaaaagtggc aatagtggaa cgacaactat
    45841 taggaggtac ttgtctccat aaaggttgta ttcctactaa atcgctgctt aaatctgctg
    45901 aagtatttca aacagtaaaa caggcagcaa tgtttggcgt tgatgtcaaa gatgctaatg
    45961 ttaattttga aaatatgttg gcacgaaaag aagacattat taatcaaatg tatcaaggtg
    46021 taaagcattt aatgcaacac aatcacattg acatttataa tggcacggga cgtattctag
    46081 gtacatctat attttcacct caaagtggca caatttctgt cgaatatgaa gatggtgaat
    46141 cagacttatt acccaaccaa tttgtattaa ttgcaactgg ttcttcgcct gcagagttgc
    46201 cattcttatc atttgatcat gataaaattt tatctagcga tgatatatta tcattaaaga
    46261 cgttaccatc aagtattggt attattggtg gtggtgttat cggaatggaa tttgcatcat
    46321 tgatgataga tttaggtgtt gacgtaacag ttatagaagc aggtgaaaga attttaccaa
    46381 ctgaaagtaa acaagcttca caactattaa aaaaatcatt gtcagcacgt ggtgttaaat
    46441 tctatgaggg aataaaactt tctgaaaatg atataaatgt taacgaagat ggtgttacgt
    46501 ttgaaatttc ctcagacata attaaagtag ataaagtgtt gctatcgatt ggtagaaaac
    46561 ctaacacatc agatattggt ttaaacaaca ctaaaataaa actttcaaca tcaggacata
    46621 ttttaacgaa cgaatttcaa caaactgaag ataaacatat ttatgcagct ggtgattgta
    46681 taggaaaatt acaattggca catgttggat caaaagaagg tgttgtggct gttgatcata
    46741 tgtttgaggg gaacccaatc ccagtaaact ataacatgat gcctaagtgt atttattcac
    46801 aacctgaaat tgcttctatc ggtttaaaca ttgaacaagc aaaggcagag ggaatgaaag
    46861 ttaaaagttt taaagtacca tttaaagcaa ttggtaaagc agtgattgat agccatgaca
    46921 caaacgaagg gtatagcgaa atggtgattg atcaatcaac tgaagaaatt gtgggtatta
    46981 atatgattgg tccacatgta acagaattga ttaatgaggc atcactgtta cagttcatga
    47041 atggctcggc attagaatta ggactaacaa cacacgcaca tccttccatc tctgaagtgt
    47101 tgatggaatt aggattgaaa gcagaaagta gagctattca cgtataaata ggaggattta
    47161 acatgattga ttataaatca ttaggcctta gcgaagaaga cctaaaagta atatataaat
    47221 ggatggattt aggtagaaaa atagatgaaa gactgtggtt attgaaccgt gcaggtaaaa
    47281 ttccatttgt tgtaagtggt caaggacaag aagcaactca gattgggatg gcatacgcat
    47341 tagaagaagg tgatattaca gcgccttatt atagagatct agctttcgtt acttatatgg
    47401 gtatctcagc gtatgatacg tttttatcag cgtttggaaa gaaagacgat gttaactctg
    47461 gtggaaaaca aatgccatca cactttagta gtagagctaa gaatattttg tctcaaagtt
    47521 caccggtagc aactcaaata ccccatgctg ttggtgctgc tttagcttta aaaatggatg
    47581 gtaagaagaa aattgcaaca gcaactgttg gtgaaggtag ctcaaaccaa ggtgacttcc
    47641 atgaaggttt aaacttcgcg ggtgtacaca aacttccttt tgtatgtgtc attataaata
    47701 ataaatatgc aatttcagta cctgattcat tacaatacgc agctgaaaaa ttatcggata
    47761 gagcacttgg ttacggtata catggtgaac aagtagatgg taatgatcca ttagcaatgt
    47821 ataaagcgat gaaagaagca agagatcgag ctatttctgg tcaaggttca actttgattg
    47881 aagcagtaac aagtcgtatg acagctcatt catcagatga tgacgaccaa tatcgtacga
    47941 aagaagagcg tgaaacgctt aaaaaagcag actgcaatga aaagttcaaa aaagagttgc
    48001 tttcagctgg cattatcgat gatgcttggt tggcagaaat agaagcggag cataaagaca
    48061 tcatcaataa agcgacaaag gctgctgaag acgcgccata tccaagtgtc gaagaagcct
    48121 atgcatttgt gtacgaagaa gggagtctta actaatggct aaattatctt atttagaggc
    48181 gatacgccag gcacaagact tagcattaca acaaaataaa gatgtgttta ttcttggtga
    48241 agatgttggt agaaaaggtg gcgtatttgg tactactcaa ggactgcaac aaaaatatgg
    48301 tgaagacaga gttatcgata cgccattagc tgagtcaaat attgttggaa ctgccattgg
    48361 tgcagctatg gtaggtaaaa gaccaattgc agaaattcaa tttgcagatt ttattttacc
    48421 tgcgacaaat caaattatta gtgaagctgc gaaaatgcgt tatcgttcaa ataatgattg
    48481 gcaatgccca ttaacgattc gtgcaccatt tggtggcggt gttcacggag gactatatca
    48541 ttcacaaagt attgaaagta tatttgcttc atcgccaggt ttaacaattg ttattccttc
    48601 cacaccgtat gatgctaaag ggcttttatt atcatctatc gaatcaaatg atccagtatt
    48661 gtactttgag cataaaaaag cttatcgttt cttaaaggaa gaagtgccag aagaatatta
    48721 cactgtacct ttaggaaaag cagatgtgaa acgtgaaggt gaagatttaa cagtattttg
    48781 ttatggatta atggtcaatt attgtttaca agcagcggat attttagctg cagacggtat
    48841 caatgttgaa gtcgttgatt tacgaactgt ttatccatta gataaagaaa caattattga
    48901 tcgtgctaaa catacaggta aagttttact tgtaactgaa gataatttag aaggtagtat
    48961 tatgtcagaa gtgtcagcga ttattgcaga gcattgcttg ttcgatttag atgcaccaat
    49021 catgcgttta gctgctccag atgtaccatc tatgccattt tctcctgtat tagaaaatga
    49081 aattatgatg aatccagaaa aaatcttaaa taaaatgcgt gaattagcag aattctaggg
    49141 agggaaagtc atggaaataa caatgcctaa gttaggtgag agtgttcatg aaggcaccat
    49201 tgaacaatgg ttagtttctg ttggtgatca tattgatgaa tatgaaccat tatgtgaagt
    49261 tattacagat aaagtgacag ctgaagtccc ttccacgata tcaggaacaa ttacagaaat
    49321 tttagttgaa gcggggcaga cagtagctat tgatacaatt atctgtaaaa ttgaaactgc
    49381 tgatgaaaag acaaatgaaa caactgaaga gatacaagca aaagtggatg agcatactca
    49441 gaaatctact aaaaaagcta gtgcaacagt ggaacagaca tctactgcta aacaaaatca
    49501 accacgtaat aatggtcgct tttcacctgt tgtatttaaa ctcgcttcag agcatgacat
    49561 tgatttatca caagttgtag gtagtggatt tgaaggtcgt gtaactaaga aggatataat
    49621 gtcagttatt gaaaatggtg gtaccacagc tcaatctgac aaacaagttc aaacaaaatc
    49681 aacatcagta gatacatcaa gtaaccaatc atctgaagac aatagtgaaa acagcacaat
    49741 accagtaaat ggtgtgcgta aagcaattgc gcaaaatatg gttaatagtg taacagagat
    49801 tccacatgca tggatgatga ttgaagtaga tgctacaaat cttgtgaata cgagaaatca
    49861 ttataaaaac agctttaaaa ataaagaagg atataatcta acgttctttg ctttctttgt
    49921 aaaagctgta gcagatgctt taaaagcata tcctttatta aatagtagct ggcaaggaaa
    49981 tgaaattgtc ttacataaag acattaatat ttcaattgct gttgctgatg aaaataaatt
    50041 atacgtacct gtgattaagc atgcagacga aaagtcaatc aaaggtatag ctagagaaat
    50101 taatacttta gcaacgaaag cgcgtaataa gcaattgaca gctgaagata tgcagggcgg
    50161 tacatttacg gtaaataata ctggtacatt tggttcagta tcatcaatgg gtattataaa
    50221 tcatccacaa gcagcgattt tacaagtaga atcaatcatt aaaaagccag tagtaattaa
    50281 tgatatgatt gcaattcgta acatggttaa tttatgtatt tcaattgatc atcgtatttt
    50341 agatggttta caaacaggta aatttatgaa tcatattaaa cagcgtatcg aacagtatac
    50401 tttagaaaat acaaatatat attagtgata acatagatgc atctatcgac aacttgtttt
    50461 atcttgttct tgtcgatgga tgtattattt tttggcacta aaatatgtgc aatatattca
    50521 aaaaagataa agaacaataa tcaacatggt tgaatgcatt tttgcagtaa agtcaaaata
    50581 agacatcata cttgaaacat attaatgaaa acatgtgaac aaattagtta ccatgatttt
    50641 aagcacaata atgtttggta tattgttaaa attgtgtcta aatataggtg tgattcagat
    50701 tagtttattg aacaatatgt tattaattag tagaatgagg atagtttaaa tataaaggga
    50761 taggtgattg aacttatgga catgaatttc gatttataca tgaacggtgt tgtagaacaa
    50821 gcaaggaatg aaattgaatc tgcgggatat gagcaattaa ctactgcaga agatgttgac
    50881 aaagttctta aacaagatgg tacaacacta gttatgatca attctgtatg tggttgtgca
    50941 ggtggtatcg caagaccagc agcatcacat gctttacatt atgacgtatt acctgatcgt
    51001 ctagtgacag tatttgctgg acaagataaa gaagcgacac aaagagcgcg tgaatacttc
    51061 gaaggttatg cgccttcaag tccgtcattt gcattagtaa aagatggaaa gattacagaa
    51121 atgattgaaa gacatcaaat cgaaggtcat gatgtgatga acgtaattaa tcaattacaa
    51181 acattattca ataaatattg tgaagaaaga taagaggcgc taacccatgt taaagttaaa
    51241 tccttacaag attggattta gaacaataaa aacagcagtg ggtatgactt taggtgtaat
    51301 tattagtaag ctgttaggtt tagataatta tgcttcaagc gccatattag tcgtattatg
    51361 tattaaacat acaaaagtac attcgctaca agcgattatt tcaagattag tatcatgttt
    51421 tttagtattg tttttaggtt cagcaatatt tagtttatta ggtcagagtc caattgtact
    51481 cggtattatc gtattgttat ttataccatt aactgtcgta ttaaaagtac aagaaggtgt
    51541 cattacgagt tgcgttatat tacttcatgt ttttaatgca aaatcaattg atgcacattt
    51601 aattgttaat gaaacattat tactgttaat tggactaagc attgcattta caatgaattt
    51661 aatgatgcca agtttagaca aacaactaga cgaatacaaa tgtaaaattg agcaacaaat
    51721 tgctgatatt tttagtaaat atagttatat ttgtgaaaaa tatgaagata ccattgcgat
    51781 tgaatttgaa gtgttacttt taaatattaa aaaggcgaag tctatcgcgt tccgagatgt
    51841 taaaaatcat tttgttagaa acgaaaattc atactatcat tattttgata tgcgagaaga
    51901 gcaagtggaa ttgttaatga gaatgaaacc gctcatcgaa agtatctgtc ataaagatcc
    51961 gcttttaaat gatgttgcgt cactgctgaa agaaattggt ctaaatgtga atagtaatga
    52021 ctatacagca atgcgtttac acaatttgta tgagacgcgt ttgaaattgg atgaactgcc
    52081 attgccaaca acacacaaag cgcttaactc gagagcaagt attattcaaa tattaaatga
    52141 attagaagaa tatttacaaa tcaaatcgca atttggttca ttgaaaatgc atagtgaagt
    52201 agcttaaaaa ataatccatc cgtatgagcc atgtttgaaa gtaatcgttg caatacgata
    52261 acttattaat acatgcgttt ataatggatg gatttttaat tgtatgattt tgtataggta
    52321 gttgagatat agtttataaa attggaatga tactcgttaa taacaatgct aatacaactg
    52381 atacaagcat aacgataatg ataattctta aaactttatt ttgcattttg tgtgcctcca
    52441 aatgaataac ctttgtatta ataattacga cataagatat aaaactttta acttgagacg
    52501 atataactag caatcatcat aaaggaatat aataaaaaac atgagtaagt ttgcttatgt
    52561 cattttcctc attatacgat atctttgaaa aatcaatcaa tatatttttg acaaagctta
    52621 gtttgagtta caataattat atataataaa ttgaaaataa tatgacacaa tttacaaacc
    52681 tgataaatct gtgtaacgtc aattactgta acattttatt tcatcaattt ttaccgtttg
    52741 gcgtacatga aaattttgat aaaaggggtt ccattatgat taatgagcaa agattactta
    52801 atacgttttt tgaacttgta caaatcgatt ctgagacagg gaatgaatca acaatccaac
    52861 ctattttaaa agaaaaattc atagcattag ggttagatgt taaagaagat gaagcggcta
    52921 aacatcctaa attaggtgcc aataatctag tgtgtacaat gaatagtact atcgaagaag
    52981 gcgaagtacc taaattatat ttgacgagcc atatggatac tgttgtaccc gcaattaatg
    53041 taaagccaat tgtaaaagat gacggctaca tatactctga tggtacgact attctaggtg
    53101 cagatgataa agcaggatta gcagcgatgc ttgaagtttt gcaagtgata aaggaacaac
    53161 aaatcccaca tggacaaatt caatttgtga ttactgttgg agaagaatca ggtttaattg
    53221 gtgctaaaga attaaattca gagttgttgg acgcggactt tggttatgct attgatgcaa
    53281 gtgctgatgt cggtactacg gttgtaggtg caccgacgca aatgttaatt tcagctaaaa
    53341 tttttggcaa aacggctcat gcaagtacgc caaaagaggg tgttagtgct attaatattg
    53401 cggcgaaagc aattagtcga atgaaattag gacaagttga tgaaataact actgccaata
    53461 ttggtaagtt tcatggcggt tcagctacga atattgttgc agatgaagtc attttagaag
    53521 cagaggcacg ttctcacgat ccagaaagaa taaaaacaca agtcaaacat atgactgatg
    53581 tgtttgaaac tactgcatca gaattaggtg ggaaagcagt agttacagtg gagcaaagtt
    53641 atccaggatt taaaattaat gataatgaag cggttgttaa aattgcacaa gaaagtgctc
    53701 gtaatttagg attatctgca aatacaatta tttctggtgg cggttcagac ggcagtatta
    53761 ttaatacatt tggtattcct tcagtcattt taggagtggg ttatgaaaaa atccatacaa
    53821 caaatgagag aatgccgatt aaatcattga acttgcttgc aagtcaagtg ttagaaataa
    53881 tcaaaatagt tgcgcgacat tcgaaatgat ttagtgtagt gtatgtctat tttgtgatac
    53941 aatactaatg aaaaatcaac aagtgaggta agtaatatga cacaacaaat tggagttatt
    54001 ggtttagccg ttatgggtaa aaacctagct tggaatattg aatcacgcgg atatagtgta
    54061 tctgtattta accgctcaag tgaaaaaact gatttaatgg ttgaagaatc aaaagggaaa
    54121 aatattcatc caacgtattc attggaagaa tttgttaatt ctttagaaaa accacgtaaa
    54181 attttattaa tggttcaagc aggtaaagct acggacgcaa cgattgatag tttgttacct
    54241 ttattagatg atggagatat tttaattgat ggtggtaata caaactatca agatactatc
    54301 agacgtaata aagcattagc acaaagtgca atcaacttta ttggtatggg cgtttctggt
    54361 ggtgaaattg gagcgttaac tggtccgtca ttaatgccgg gtggacaaga agaagcttat
    54421 aacaaagttg cagatattct tgatgcaatt gctgcaaagg caaaagacgg tgcttcatgt
    54481 gttacttata ttggacctaa tggtgctgga cactatgtga aaatggtaca caatggtatc
    54541 gaatatgctg acatgcaatt aattgcagaa agttatgcta tgatgaaaga attattaggc
    54601 atgtctcatg aagacattgc tcaaacgttc aaagactgga atgctggtga attagaaagt
    54661 tatttaattg aaatcactgg tgatattttc atgaaattag atgaaaataa agaagcatta
    54721 gttgaaaaaa ttcttgatac tgcaggtcaa aaaggtacag gaaaatggac ttcaattaac
    54781 gcattagaat taggtattcc attaacaatc attactgaat ctgtatttgc acgttttatc
    54841 tcttcaatca aagaagagcg cgtaaatgct tctaaagaat taaatggtcc aaaagcatca
    54901 tttgatggtg acaagaaaga ctttttagaa aaaattcgta aagcattata tatgagtaaa
    54961 atttgttctt atgcacaagg tttcgcgcaa atgcgtaaag caagtgaaga taatgaatgg
    55021 aatttaaaac ttggtgattt agctatgatt tggagagaag gttgcattat ccgtgcacaa
    55081 ttcttacaaa agatcaaaga tgcttacgat aataatccag gattacaaaa cttattgcta
    55141 gatccttact ttaaaaacat cgttactgaa tatcaagatg cgttacgtga tgttgtcgca
    55201 actggtgttc aaaatggtgt accaacacct ggtttctcat caagtattaa ttactatgat
    55261 agttaccgtg ctgctgattt acctgcaaac ttaattcaag cacaacgtga ctacttcggt
    55321 gctcatacgt acgaaagaaa agataaagaa ggcgtattcc atacacaatg gattgaagaa
    55381 taatattgat taaaggatag ttgaaagata ctatcctttt ttatatcttt tgataaatat
    55441 aaaagagagt gtgatagagt agctacaacc tataaaaaat tacacaagta ttatccttga
    55501 ccttaaaagc atcccgccat atttttaagc attttgtgaa gtaataaaat atttactgtg
    55561 ttatgatagc atgtactata gagaaataga ggtactattt tatggaaaag ttaaatcaag
    55621 ttatgttata tgttgatgat gtcgaaaaag cgaaagcatt ttggacagaa actttagaat
    55681 ttgttgttgt aagtgaaaca ccgttagctg aggactatgt tgcagttgaa gtatcaccta
    55741 ccaaagatgc tgaaacgtca ttgacgatta tggcaaaaga atttattgaa aagcatagcc
    55801 cggaagtgaa cctaggaaca ccatctttaa tgtttaaaga gaaaaacttt gatgcattat
    55861 actcaaaatt aaatgattta ggtttaactg ggcatgatat agtagaaatg aatggtcaac
    55921 gtgtatttaa ttttcaagat ggccaaggta actattttgc agttagtgac taaacaatcg
    55981 attttaatat ttatacaaac ctttatattg attttcggat tgaatttcag tatgaaggtt
    56041 ttatttttat cgttttatgc aatcgtttgc acaaatcttt tgactttttt agaaagtgtg
    56101 actaaactgg tattatacag tggttggaag cgttttccaa tgcagaaatt aacaggaggt
    56161 gattaaatgg ttacgattaa agatgttgca ctaaaagccg gtgtttctcc ttcgacagtt
    56221 tcaagagtta taaaaggaaa tcaacgtatt agcgaagcga caatttcaaa agtgaagaaa
    56281 gttatggaag aattgaatta ttttcctaat accgctgcta gaactttaat tacaaaccaa
    56341 acatataaaa ttggtttagt gttaaaaggg tctgaggagc ctattcgact gaatccattc
    56401 tacatcaatg tattgctagg aatttctgaa acgtgtaacc agcatggcta tggtacacaa
    56461 acgacagtct caaataatat gaatgattta atggatgaag tttataaaat gattaaacaa
    56521 cgaatggttg atgcgtttat actgctctat tcaaaagaaa atgatccgat taaacaaatg
    56581 ttaattgatg aaagcatgcc atttattgtg attggtaagc ctacatcgga tatagatcat
    56641 caatttacac atatagataa tgataatata ttagcttctg aaaatttgac acgacatgtt
    56701 attgaacaag gtgtagatga attaatattt attacagaaa aaggaaattt tgaagtttca
    56761 aaagatagaa ttcaaggatt tgaaacgata gcttctcaaa ataaaattaa ttatcaaatt
    56821 attgagacta gtaatgaaag agaagttatt tttaattaca tgcaaaatct acatacgcgt
    56881 ttgaaggacc caaatattaa acaggcaatc atttcgttag atgctatgtt acatttagcg
    56941 attttaagtg tcctatatga acttaatatt gaaattccga aagatgtaat gacagcaacg
    57001 ttcaatgatt cttatttaac cgaaattgcg tcaccaccac aaacctgtat tgacatcaag
    57061 cctcgaatgt taggtcaaca ggctggtgca gcaattttaa atatattgaa aaacaaagca
    57121 caggatgtta ttgaactcgt cattatagat acagaattaa aaataagaaa atcaacacag
    57181 cgatagcaaa ggagttttaa aatgaataag caatggtgga aagaagcagt agcatatcaa
    57241 gtatatccaa gaagttttaa tgatagtaat cacgatggta ttggggattt acctggaatg
    57301 attgataaat tggactactt aaaagatttc ggtatcgatg tcatttggct cagtccaatg
    57361 tttaaatcac ctaatgatga caatggttat gatattagtg actaccaaga gattatggat
    57421 gaatttggaa cgatggaaga ctttgatcgt ttattaaaag gtgttcatga tagaggcatg
    57481 aagcttattt tagatttagt tgtaaatcat acatctgatg aacatccttg gtttatagaa
    57541 tccaaatcta gtaaagacaa tcccaaacgt gattggtaca tttggcaaga tccaaagcca
    57601 gatggctctg aacctaacaa ctgggaaagt atatttaatg gatctacatg ggaatatgat
    57661 gctaatactg agcaatatta tttccattta ttcagtaaaa aacaacctga tttgaattgg
    57721 ggtaatccgg aagttagaga tgctgtattt gaaatgatga actggtggtt tgataaaggc
    57781 attgatggat ttagagtaga tgcaattacg catattaaga agacgtttga agcgggtgac
    57841 ttacctgtac ctgagggtaa aacatatgcc ccagcatttg atgtagatat gaatcagcca
    57901 ggtatacaaa cttggttaca agagatgaaa gatcgctcat taagtaagta tgacattatg
    57961 actgttggtg aagcgaatgg tgtaagccct gatgatgctg atgactgggt cggggaagaa
    58021 aatggtaaat ttaatatgat attccaattt gaacatttgg gactgtggaa tagtggtgat
    58081 tctcactttg atgtaaattc gtataaatct gtattaaata gatggcaaaa acaacttgaa
    58141 aataaaggtt ggaatgcgtt gtttattgaa aatcacgacc aaccacgacg tgtatcgacg
    58201 tggggtgacg atgacaagta ttggtatgaa tcagcaacaa gtcatgcagc tgtttatttc
    58261 ttgcaacaag gtacgccatt catttatcaa ggtcaagaaa ttggtatgac gaattatcca
    58321 tttgaaagta ttgaaacgtt taacgatgtt gctgttaaaa atgactatca aatagtgaaa
    58381 gctcaaggtg gagatgtaga cgctttactt gcgaaatata aagatgagaa ccgagataat
    58441 tctcgcacac caatgcaatg ggatgatacg ttaaatggag gatttacaaa tggtgaaccg
    58501 tggttcccag tgaatccgaa ttataaaact atcaatgttg cacaacaatt agaagatgag
    58561 cattcagtat tacaatttta taaagattta attcaattaa gaaagtctaa tgatgtatac
    58621 gtatatggtc aatttgattt agtagatgct gaaaattcac aagtttttgc gtacacgaga
    58681 acattaaatg aaaagcaagt tcttattgta ggtaatctta ctaaccacga agcagaatta
    58741 actgtaccat ttgatttaag ccatggagaa gtaaagctat ttaattatga tgccaaagtt
    58801 aatttaaaac agttacgtcc atatgaagca tgtgttatcg aactaaatta acatattaat
    58861 atgcccccta atagaatcta aggccatttg tagacctata gatatattag ggggcttctt
    58921 tgttttttat tatttaatct tcgtcataag gactctgttt aacaggaatc caaagctgaa
    58981 ttttagtgaa tgggtcatta aatgaaatat cgagagggta cacttcaaca tataaatcat
    59041 ttcgttcata tggcaatgtt aactgcaaac ttgattcaat atagtaccaa gcttcattag
    59101 ttgcataatc aatttcacct tgtaaattga atttcgcaca atgtttgcca ggtaaaaatc
    59161 gactttctaa gtgtgcagga taacgttcac ttggtacacc tacaaatatt tctaaaccat
    59221 tttcaagagg acaactaata acaaatagtt caaatggact aacgtcatta tatcgtcgta
    59281 actctttaat tttaccatca atgagcaagt cttctaaaaa atccggaaca ttaaaaggat
    59341 gtgacaaata cttagtgtct ataaatcgtg catatccaac caatgaaata tcatctgtct
    59401 cttctaatct gtatggataa ggtgctctct cagtagttga taatttgata tataatcgct
    59461 cttgaatttg taattcatct tttttagtag aggcttgaat aggtgatacg ccgtgaaaat
    59521 cactaaaatc atttgcaaaa tcatttgaat ttgcatagtg atatttttta gcgatatcta
    59581 caagtcgtgt agcaccatta atcacatcat ttgcagcgag tgtcatttta cgcgcgcgtg
    59641 cataagcttc tggagataag ccgacaatca ttttaaatga ttgatcaaga tggtatggcg
    59701 aaagaccaac gtaatcactt aattcttgca aattgaaagg ctctaataaa cgatcttcaa
    59761 tataaacaat tgcctgttgt atttgcttga taacgtccaa aactttcact ccaattaatt
    59821 caatcattta tttttattct acattatttc tataaattat acacccattt gttcaatgat
    59881 tattaaaata gttttgggca ttgtaaaata taatttcata atatagtcta gaaaaaaagc
    59941 gaatgataga acaattgatt tacttgattc gtaatcaatc cttgtcattc gctcatttat
    60001 ttttgtttaa catgtgcgtt ttaattcaat tattgaatat cgtcccacca atggttacca
    60061 tcacgagcaa gtagtaaatc actttctaat ggaccattag tacctgattc atagttaggg
    60121 aattctggat caaccatatt ccattcatct tgaattgcat caacaaattt ccatgttgat
    60181 tttaattctt cccagtgcgt gaagttagtg gcatcacctt taagacaatc aaataataga
    60241 ttttcatatg catctacagt attcatttta tcttgagcgc tcattgagta agacaattgg
    60301 acaggttctg tttcgatacc ttgtgtattt ttcttagcat ttagatgtaa agatacacct
    60361 tcattaggtt ggatattgat tactaatagg tttgaatcta acagtttatc agtttcatag
    60421 tataagttca ttggtacttc tttaaattca acgacaactt gaattgtttt agatttcata
    60481 cgtttaccag tacgaatata gaatggtaca ccagcccatc taaagttatc aattgttaat
    60541 ttacctgaaa caaaggtagg tgtgttagag tcatctgcaa cgcgatcttc atcacggtat
    60601 gctttaactt gtttaccatc gatatagcct tcgccatatt gaccacgaac aaagttcttt
    60661 ttaacatctt cagattggaa atgacgcagt gatttaagta cttttacttt ctcagcacgg
    60721 atatcttcac tatttaaact aataggtgct tccatagcta ataatgcaac catttgtaac
    60781 atgtggtttt gcaccatatc ttttagcgcg ccacttgatt cataataacc accacgatct
    60841 tcaacaccta gtatttcaga agatgtaact tggatgtttg aaatatattt gttattccat
    60901 aatggttcaa acatcgcatt cgcaaaacgt aatacctcga tattttgaac catgtctttt
    60961 cctaaatagt ggtcaatacg ataaatttct tcttctttaa atgatttacg aatttgattg
    61021 tttaatgctt cggctgattt taaatcacta ccgaatggtt tttcgataac aaggcgttta
    61081 aatccttttg tatcagtaag accagaagat tttagataat cagaaataac gccaaagaat
    61141 tgtggtgcca ttgctaaata gaatagtcga ttacctttta attcaaattg gctatctaat
    61201 tcattactaa aatctagtaa ttcttgatag ctttcttcat tactaacatc atgtctatga
    61261 tagaagacat gttccataaa cgcgtcaatt ttgtttgtat cttttacgtg cttttgaatt
    61321 gatgatttta cttgattacg gaaatcatca ttagtaatgt cacgacgtcc aataccgatg
    61381 atggcaatat gttcatctaa attgtcttgt tggtagagat ggaatattga tggaaacaac
    61441 ttacgatggc ttaagtcacc agttgcacca aagattgtga ttaaacatgg gatgtgtttg
    61501 tttttagtac tcaagattaa aacctcaatt cttaattaga tatatgattt attattataa
    61561 gataatccat tcagtaggtc atacaatatg ctcgactgta tgcagtgtct taaatgaaat
    61621 atcgattcat gtatcatgtt taatgtgata cttattaatg ataagtataa cgtaattatc
    61681 aaaatttata tagttatgtc tacgttaaag ttagaaaaat taactagcaa agacgaattt
    61741 ttaacagatt ttgattcaag tataaattaa aactaaattg atacaaattt tatgataaaa
    61801 tgaattgaag aaaaggaggg gcatatatgg aagttacatt ttttggaacg agtgcaggtt
    61861 tgcctacaaa agagagaaat acacaagcaa tcgccttaaa tttagaacca tattccaatt
    61921 ccatatggct tttcgacgtt ggtgaaggta cacagcacca aattttacat catgcaatta
    61981 aattaggaaa agtgacacat atatttatta ctcatatgca tggcgatcat atttttggtt
    62041 tgccaggatt actttctagt cgttcttttc agggcggtga acagaagccg cttacattgg
    62101 ttggaccaaa aggaattaaa gcatatgtgg aaatgtctat gaatttatca gagtcacatt
    62161 taaactatcc aattacatat attgaaatcg atgatcatct gacatatcat catgatggtt
    62221 ttactgtaga ggcgcattta cttaatcatg gtattccatc atatggatat agagtaatgg
    62281 cacctgaaac tacaggtaca atcaatgttg aagcgttaaa aaacattggt cttgaaccag
    62341 gtcctaaata tcaagaagta aaatctcatg atacatttga acataatggt caagtttatc
    62401 aatcaaaaga ttttagaggt gagtccaaac agggtccagt tgtagcaatt tttggtgata
    62461 caaaaccatg ttctaatgaa cgtgtgattt ctagagatgc tgatgtgatg gtacatgaag
    62521 cgacctatat agatggtgaa aagcatttag ccaacaatta tcatcatagc catattgaag
    62581 acgtatttgc attgataaaa gaagccaatg ttaaacgtac gttgattaca catttgagca
    62641 atcgctataa tacagaagat ataaatgaaa tttatcaaac attgatacaa aatgaagata
    62701 cacctaattt taattttgta aaagattttg atagttttaa aatctaatga caaagtaata
    62761 aacaatgctt gccaaataac aataaattat aaaaataacc cgtcaaacac agtgcttgcg
    62821 atgatgcata catgtgttgg cgggtttgtt tttattggtc ttctatatta gaaagttcaa
    62881 tactacggtc gacggcagcg tttagacaat cttcgaaaat agatactaaa tcatattgtg
    62941 acaatgtatc aaggccagct tgtgtcgtac cacctttaga ggtaatattt tttcttaatt
    63001 gagccatgct caaatctgaa cgttcaatca tcttacttgt acctataata aggttgcgta
    63061 tagattcttc aacttgttct ttttctagac caagtttcgt accagcttta acatattgct
    63121 cgaatacatg atataaaaat gctgggccgc ttccggtgat agctgttact tgatgtaaat
    63181 gatcttctga tacttcaatt acagaaccaa atgctttaac taaatcgtta atttcatctt
    63241 tagatttagg gtcaaagttg tttgaaaaac taatgccagt aacagagtgt ccaacttgcg
    63301 catttgtgtt tggcataatt ctagcaactg gattttggca ttctaattgt tgtttaatat
    63361 aatcaatcgg aatacctgcc ataattgaaa tgaagcaatt gtcttttgta atatgtggtt
    63421 tgatgcgtgt tgctagagca tcaaaatcat gtggtttggt acctaaaaat acataatctg
    63481 catcttttaa taatgtcgca tcatcataac tatagttaac acctagtttt tcagcgaatg
    63541 cttttaaagc ttgttcatta gatttatttg ttaaatatat atcattggca tctaagttgc
    63601 ttgagttaat aattcctgta aatatagctt gtgccatatt accagctcca taaaatacga
    63661 gtttcattaa cgtcacatcc ctttagtttt tgatttgtat gttatatttt cttattattg
    63721 tatatatatt aggaaagata atcaacttgt ctcaattatg taaagttgat aaattgcatt
    63781 ctatataatt gaaaaggaag gttaattgat gaaaggacaa cattttatag tgactggtgg
    63841 tacgagtggt ttaggtttat cgattgtccg aaagttacta gaaaataaag tgcatgtaac
    63901 gttgttggtg cgtgatgttg ataaagcaac tagaatcttt gaacaggaat tggggaaaac
    63961 tataaatgtt attccttgtg acttaaatga tttgaagtcg atacaggcac tgcaatttga
    64021 agacaataca tcattcgatg gattcattta tagtgcaggt ttaggttatt tcaaatctat
    64081 ttcagaccat agtttttcag aaatgattga aacgtatcag cttaatttaa ttagttttaa
    64141 tgttttatat actgtgttga gaccatattt aacatctaac gcacatattg taggtatttc
    64201 aagtcaagct gcattttcca cacaagcaaa tgctgcacat tatggggcat cgaaagcagg
    64261 gttttatgca ttaatgaatg ctttaagact agagtcacca aacttacata tcatgacagt
    64321 taatgttggc ccgattgata caccttttca tcaaaaagcc gacccatcaa tgaagtatgc
    64381 taaaaagatg ggggaaatta tgcttgatgc taatcagctt gcagaagata tcatttatgg
    64441 cattaaaaca aaacaattgg aaattaatcg gcctaaatgg atgcatcatg cgttgaaaat
    64501 gtatcaaatt gcgcctagat tttttgaacg atgtttccct aaattattta aaaataaagc
    64561 ataaataggt gggatggcaa tgaactgtca atcggagagt gtggtaagac tttgtgtacg
    64621 atatgcggaa caactatcag tatttgaaga atttacagtg ctggatattt taggcgatat
    64681 ttctgtagac caaatttcag acggtacatt atattatact tgtgaaaaat tcaaactact
    64741 tgtgttgcaa ggaaatgtgc tgggtgtaca aataataact aataatgatg aatcaacatg
    64801 cgaagtgaaa tatagaaaga tgttttaata aaattattaa gagatgaagt ggcagaagtt
    64861 tgcttaaggc aatgccgcaa ggcgaaatta ctaaatactg atgcgtgagg acaacgcttt
    64921 aatttctatc ataagtgagt aatcataatc ataaattata gcgaattgta cataaacaac
    64981 agcagtaagg catttttata atgaaacgcc tcatgctgct gtttttatta ctgaaaatga
    65041 cttctacact aaatgattgg tatattccaa atcctttaca cgagctcttg ctgttttaat
    65101 ctgatctaaa ctaactttag tatgatagtt ttcaatattt tcttttaatt ggtcgacgct
    65161 acttgcacct acaatgatgg aaccaagcac gtcatgtgat gttaaataac taaatgtcaa
    65221 tgcagataaa ttactttcaa tttctttaat agaggctatt gtttcaccca attcatcatg
    65281 agaataatca aaaataccat ctttaaattt attgtcgagc acattaacac tgtttgaagt
    65341 taataatcct ttagacacag gtcctcttgc caatacttta acatcattgt tgtgaattgc
    65401 atctaataat gattctggac gattatcaat caaattgaat tgagacatta tcgtttcgat
    65461 ttgactatgt tttaaataat aatcaattac atttgggcga atagaagaaa taccgtaagc
    65521 acgtataatt ccttcttgtt tcaattcatc aaatgcgctt attgtttcgt ctaatgggtc
    65581 atcaatggtt ccgccatgaa gttgatataa atcgatatga tcgataccta aacgctttag
    65641 tgaaccttta actgcctctt taatatagga tttactcgga tcccatgttg tactgccatc
    65701 ttttgttaaa cgattgccta cttttgtacc aataaaaata tcatcgcgtt gttgatattt
    65761 aagaagtgcc ttaccaacaa ctgattcatt tataccttta tcgtacatat ctgctgtatc
    65821 aaaataagtg ataccatttt caacagcaca atctataatt tgttcggctt tttttaaatc
    65881 tgtacctaaa ctcatgcaac ctagacctaa ttcagataat gaaataccac tttttaatat
    65941 atttttttgc atgtctgttc tcctttcgat acactttagc taacaagcat agtgtatcaa
    66001 tatataatat gaatttaaag gaacgtgatt ataatggatt taaatgaaaa aacaattgat
    66061 cgaacagtta tttataatgg caaaattgta gatgtagaaa ttcatacagt gacattacca
    66121 aacggtgaaa cgtcaacaag agaattagtt tatcataatg gtgcagttgc tgtttgtgca
    66181 gtgacaccta aaaaagaagt cgtattagtg aaacagtatc gtaaaccagt agaaaaacca
    66241 ttgctagaaa ttccagctgg taaattagaa gatgatgaag atagagtcga agcggcaaaa
    66301 cgtgaattag aagaagaaac aggatatatt gccaaagagt taacacatgt tgtagatatg
    66361 tatggttcac caggtttttg cgatgaacaa ttatcaatat attttacgga taatttagaa
    66421 gaaggtacag ttcacttaga tgaagatgaa tttgtcgaag tcattaaagt tccgattgag
    66481 aatgttaaat cgatgctaat gaataaagaa atagaagatg cgaagactat cattgcattg
    66541 caacacctat tattaaatta taatcattct aaataacaaa tgagaaaagc ttgcatttta
    66601 ttgagaaaac tgttagtttt aattgtgaag tttgaaataa tttgtaatga ttttaattat
    66661 tagtagggga gtggacatcg ttggaagaac gattaaatcg cgttaagcaa caattacaac
    66721 aatcatcata taagctaacg ccacaacgcg aagctactgt tagagttcta attgaaaatg
    66781 aaaaagatca tctaagtgct gaagacgtat atctgaaagt aaaagataaa gcgcctgaaa
    66841 ttggcttggc gacagtatac agaacgttag agttgttagc tgaactaaaa gttgtcgaca
    66901 aaattaactt tggtgatggc gtcgctcgtt ttgatttaag aaaagaaggc gcaaaacatt
    66961 tccaccatca tttagtatgt atggaatgtg gtcgtgtaga tgaaatcgat gaagatttgt
    67021 taccagaagt tgaaaatcga gttgaaaatg agttcaattt taaaatttta gatcatcgtt
    67081 taactttcca tggtgtgtgt gaaacgtgcc aagctaaagg taaaggatag taaattgcgt
    67141 aggttaaatt aaccttcgct ttttttagag gtgtggttat ggaaacaatt attgaagaat
    67201 atttgcgttt tatacaaatt gaaaaaggac taagttctaa cacaattggt gcttatagac
    67261 gtgatttgaa aaagtatcaa gattatatga ctgaacatca tatctcgcat attgatttta
    67321 tagatcgaca attaattcaa gagtgtttgg ggcatttaat agaccaaggg caatctgcta
    67381 aatctattgc gcgatttatt tcaacaatcc gtagttttca tcaatttgct ataagagaaa
    67441 aatatgcggc gaaagatcca acggtattat tagattcacc aaaatatgac aaaaaattgc
    67501 ctgacgtttt aaatgttgac gaagtgttgg ctttattaga aacgccagat ttaaataaaa
    67561 ttaatggata tcgtgatcgt acgatgttag aacttctgta cgcaacggga atgcgtgtat
    67621 ctgaattgat acatttagag ttagaaaacg tgaacttaat aatgggattt gtacgcgtat
    67681 ttggtaaagg cgataaagaa agaattgtac cattaggcga cgcagtcatt gagtacttaa
    67741 ctacttatat tgaaacgatt agaccgcaac ttttaaaaaa gactgttact gaagtcttat
    67801 ttttaaatat gcatggtaaa cctttatcac gacaagcaat atggaaaatg attaaacaaa
    67861 atggtgtaaa ggcaaacatt aaaaagacgt taacgccaca tacgttacgc cactcttttg
    67921 cgacacattt attggaaaat ggcgcagatt taagagcagt gcaagagatg ttaggtcact
    67981 ctgacatatc tactacccaa ctctatacac atgtttcgaa atctcaaatt agaaaaatgt
    68041 ataaccaatt tcatcctaga gcataaagtg aacaataact caaaagtcac aatacacatg
    68101 actaaaaatg tctgtgctat tgtggctttt ttaaattggt tgattaatta cgtctatgtt
    68161 ttcttaattg aatcgcttct tcttttgctg caatcacttc tgaacgatca cggcgcatgt
    68221 gatggtctac aataaaagga tctgttgctg tttcctgatt ataatcatag tctggatagt
    68281 tggccttgat gatgcgttca aagactggag ttattggtaa tataacagat gaaaaaggct
    68341 ttgctgcatt caattttgca atctgttgct caattaacaa ctgataatca tttaaattaa
    68401 ggtataacgc atctctatct ttagcatttt gtattatttc tttagattta ttaaaagact
    68461 tataggcgcc ttttaaatta ttgcggcgat aatggtaaca agcagttgca aacaagatta
    68521 aactaacaac tgcatcttgc ttactgtagt tattttcagc tttccatgca tcttctaaaa
    68581 tgtcatgaca taggaaataa tgttgcttag tatgaaattg ataatagaaa tttatcagtg
    68641 cctgttgcat tttgttatca ccccaattta aaagtaagtt attttcatgc tataatattt
    68701 tagagaatta tgcacatatg acgcaatacg aggtagatat tatgtatgaa gttaaattag
    68761 atgctttcaa tggaccatta gatttattgc tgcatcttat ccaaaaattt gaaatagata
    68821 tttatgatat tcctatgcaa gcattaacag agcagtatat gcagtacgtt catgcaatga
    68881 aacagcttga aattaatatt gcaagtgaat acctagtatt agcgtcagaa ctcttaatga
    68941 ttaaaagtaa gatgctatta ccacaatcaa catcagatat ggatgttgat gatgacccac
    69001 gggaagattt agttgggcgt ttaatagaat atcaaaatta taaagaatat actgctattt
    69061 taaatgacat gaaagaagaa agagattttt attttacaaa aagaccgaca gatttatctc
    69121 atttggaaac agatgaatct tgggatccaa atcatacgat tgatttaact gaattaattg
    69181 tagcttatca aagagttaaa aatagagttg agttaaatac acctaaatct gttgaaatcc
    69241 gaaaagagac atttaccatt caacaagcta cagaacaagt gacatcgaga ttgaaagata
    69301 aagatcattt taacttcttt agtctgttta cgttttctga gccaattgaa caagtagtca
    69361 ctcacttttt agctatttta gagatgtcaa aagcaggaat aattaatatt gagcagcaac
    69421 gtaattttga agatattaac attattagag gagtgaacta ccattttgga taatcatggt
    69481 atattagagt cgcttttatt tacagctggc gatgaaggtt tagatgaaaa acaactatta
    69541 gaaatattag atatgtcgaa agaccaactc gttgaattaa ttgaaaatta ttcatcacat
    69601 ggattaatga tacaacgatt tggaatgacg tatgttttaa cgactaaaaa agaagcggca
    69661 acgtatattg aacaattaat tgaacaaaag tcacaaatga aattatcaca agcagcaatg
    69721 gaagtactat caattattgc ttataaccag ccattatcaa gaagtgatat tgaattaatt
    69781 cgtagtatca attcagatgg tgcagttaag acattgattg ccaaaggact agttgaggct
    69841 aaagtggtta atgaacagcg tagccaacag ttaattacta ctgatttatt tttaaatgta
    69901 tttggtattt ccaatataga agatttgccg acaactgaag aagacgatga agaaatggat
    69961 gcttttttca gtaatctagt caatcaaaaa ggagaaaata atgactaaag aattagaacg
    70021 attacaaaaa cgtatagcta atagcggtta tacttcaaga cgtaaggcag aaactttaat
    70081 ttctgaagga aaagtgaaag tgaatggtac gactgtcact gaattaggaa caaaagtgaa
    70141 gccatcagat acgattgaag ttgaaggtat taaaatagaa ctagaagaca aaatttacat
    70201 tttattccat aaaccaactc aagtgataac aagtgtatct gatgatagag gacgtacagt
    70261 ggttacagat tactttaaag atatagaagc acgtatttat cctgttggtc gtttagacta
    70321 tgatacttct ggattgttat tactcacaaa tgatggtgaa tttactaatt taatgacaca
    70381 tccaagatat caaattaaga aaaaatatgt tgcgaaatta aaaggttatt taatgagaga
    70441 agaagtgaaa gcgctagaaa aaggtattga attagaagat ggtatgacgc aaccggctga
    70501 agtgaaagtt aagaaacaag ataaagataa aaacacaaca ttggttgaaa ttacaattac
    70561 agaaggtcgt aatcgccaag tcagaagaat gttcgaacat tttggtcatc aagtgacgaa
    70621 actgtctcgt attgaatatg ggcctttaaa tgttgtcggt ttgaatgctg gtgaaggacg
    70681 tgtattgacg cctcatgaag taaaagtgat gcgtcattta gcagaacatg ggaaataatt
    70741 aaataaaata tgtatttatc acaaagtttg agaatttttt cacaaaattt taggttttta
    70801 ataactgtat gcgctttcct gtgatataat acttttgtaa agaaaagcat gtgtgggagg
    70861 tatgacctgt atgtcgaacg aaatacttat cgtagatgat gaggatagaa tcagaagatt
    70921 acttaaaatg tatttagaaa gagaatcttt tgaaatccat gaagcaagta atggccaaga
    70981 ggcttatgaa cttgcaatgg agaataatta tgcttgcata ctactagatt taatgttgcc
    71041 tgaaatggat ggtatccagg tggcaactaa attgcgtgaa cataaacaaa caccgattat
    71101 tatgttgact gctaaaggtg aagaaacaaa ccgtgttgaa ggttttgaat ctggtgcaga
    71161 tgattatatc gtcaaaccat tttcaccaag agaagtagtc ttaagagtta aagcacttct
    71221 aagaagaacg caatctacaa ctgtagaaca aagcgaacct cacgcacgtg atgtgattga
    71281 atttaaacat ttagaaatag ataatgatgc acatcgcgta cttgctgata atcaagaagt
    71341 taatttgact cctaaagagt acgaattatt aatatattta gctaaaacac caaataaagt
    71401 atttgaccgt gaacaattat taaaagaagt ttggcattat gaattctatg gtgatttaag
    71461 aacagttgat actcatgtta aacgacttag agaaaagtta aatcgtgtgt ctagtgaagc
    71521 tgcgcatatg attcaaacag tctggggcgt tgggtataaa tttgaggtta aatctaatga
    71581 tgagccggct aaatagtgtc gtaattaaac tgtggttaac tattatttta atagtgacga
    71641 cagttttaat tttattaagt attgctttaa ttacctttat gcaatactat ttcacacaag
    71701 aaaccgaaaa tgccataaga gaagatgcta gacgtataag ttcactggtc gaacaatcac
    71761 ataataaaga agaagcaata aaatatagtc aaacattaat tgaaaatcct ggtgggttga
    71821 tgattataaa taataaacat cgtcaatcaa cggcttcact ttctaatatt aaaaagcaaa
    71881 tgttgaatga agtagtcaac aacgaccatt ttgacgatgt gtttgataaa ggtaaatctg
    71941 ttactcgaaa tgtaacgatt aaagaaaagg gctcatctca aacatatatt ttgttaggct
    72001 atccaacaaa agcacagaag aatagtcata gcaaatatag tggagtcttt atatataaag
    72061 acttgaaatc aatcgaagat acaaataatg ctattacgat tatcaccata attacggctg
    72121 ttattttctt aacaattaca acagtctttg cgtttttctt atcgtcaaga attacaaaac
    72181 ctttaagacg tttaagagac caagctacac gtgtatctga aggggattac tcttataaac
    72241 cttctgtcac aacgaaagat gaaattggtc aattatcgca ggcatttaat cagatgagta
    72301 cagaaatcga agagcatgtc gacgcattat ccacatctaa aaatattaga gacagcttaa
    72361 ttaactctat ggtagaaggt gtcctaggta ttaatgagag tcgacaaatt atcttatcta
    72421 ataagatggc gaatgatatt atggacaata ttgatgaaga tgctaaagct ttcttattaa
    72481 gacaaataga agatactttt aaatcaaaac aaactgaaat gcgcgattta gaaatgaatg
    72541 cacgattctt tgttgtgacc acaagctata tcgacaagat tgaacaggga ggtaaaagtg
    72601 gtgttgttgt gacagttcgt gatatgacta atgagcacaa tctagatcaa atgaagaaag
    72661 atttcattgc taatgtatca catgaattac gtacaccgat atcattactt caaggttata
    72721 ctgaatcaat tgtagatggt attgttacag aaccggatga aataaaagaa tcgcttgcca
    72781 ttgtccttga tgaatcgaaa cgtttaaatc gtttagttaa tgaattgtta aatgtcgcac
    72841 gcatggatgc tgaagggtta tccgtaaata aagaagttca gcctattgca gcgttactag
    72901 ataagatgaa aattaagtat cgccaacaag ctgatgattt aggtctaaat atgactttta
    72961 attattgtaa gaagcgtgtt tggagttatg atatggatcg catggaccaa gtactaacga
    73021 acttaattga taatgcatca cgttatacga aacctggaga tgaaattgca attacttgtg
    73081 atgaaaatga aagcgaagat attttataca ttaaagatac aggtacaggc attgcaccag
    73141 aacatttaca acaagtattt gatcgttttt ataaagttga tgcagcgaga acgcgaggta
    73201 aacaaggtac cggtttaggt ttgttcattt gtaaaatgat tatcgaagag catggtggtt
    73261 ccatagatgt taaaagcgaa ttagggaaag gcacaacatt tattattaaa ctaccaaaac
    73321 cagaataaaa ttgaatatag ttatttcaga acgcatgtta ttgattagag actctaattt
    73381 atagcatgcg ttttttgatt gatgtgggga attttgtatg tggattagaa cttagggttt
    73441 ttgcgaatat caactattaa atatattact aatttataca aaaatataaa gtttgataaa
    73501 gttatttatt tgattataaa aatagggtaa aatatagata tattgtatta attaaattat
    73561 tcgaggtgtc atatgaaaaa attcattgga tcagttttag ctacgacatt aattttaggg
    73621 ggatgttcca cgatggaaaa tgaatcaaaa aaagacacaa aaacagaaac aaaatctgta
    73681 ccagaagaaa tggaagcttc aaaatatgta ggccaaggct ttcaaccgcc tgcagaaaaa
    73741 aatgcgattg aatttgcgaa gaagcatcgt aaagaatttg aaaaagtagg tgaacaattc
    73801 tttaaagata actttggact aaaagttaaa gctacaaatg ttgtaggtaa agatgatggt
    73861 gtagaagttt atgtgcattg tgaagatcat ggcattgtat ttaatgcaag tctacctttg
    73921 tacaaagatg ccatccatca aaaaggatca atgcgcagta atgacaatgg tgatgatatg
    73981 agtatgatgg tgggtacagt gctgagtggc tttgaatatc gagcgcaaaa agaaaagtat
    74041 gataacttat ataaattctt caaagaaaat gaaaagaaat atcaatatac aggctttaca
    74101 aaagaggcaa ttaacaagac acaaaatgtc ggatataaaa atgaatattt ttatattaca
    74161 tactcttcta gaagtttaaa agaatatcga aagtattatg aaccactgat tcgaaaaaat
    74221 gataaagaat ttaaagaagg aatggaacga gcaagaaaag aagtgaatta cgctgcaaat
    74281 acagatgctg ttgctacact tttttctact aagaaaaact ttactaaaga caatacagta
    74341 gatgatgtaa tcgaactaag tgataaatta tataatttaa aaaataaacc agataaatct
    74401 acaatcacaa tacaaatagg gaaacccact attaatacta agaaagcctt ttatgatgat
    74461 aatcgtccaa tagaatatgg ggtgcacagt aaagatgaat aaaattaatg atagggattt
    74521 aacagaatta agtagttact gggtttatca aaatattgat ataaaaaaag aatttaaagt
    74581 taatggaaaa aggtttaaac aagtagacag ttataatgat gataagaata gtaatttgaa
    74641 tggtgctgct gatattaaaa tatatgagtt attagatgat aaaagtaaac caactggtca
    74701 acagacaata atttatcaag gaacatctaa tgaggcaatt aatccaaata atccattaaa
    74761 atcatcgggg tttggagatg attggctcca aaatgctaaa ttaatgaata atgataatga
    74821 aagcacagat tatttaaagc aaacagatca attatcaaat caatataaaa taaagttaga
    74881 agatgcagat agattatcaa atagtgattt tttaaaaaaa tatagaatgg aatcaagtaa
    74941 cttcaaaaac aaaaccattg tggcggatgg cggtaattcg gaaggcggtg caggagcaaa
    75001 atatcaagga gcgaaacatc cgaatgaaaa agttgttgct actgactcag caatgattcc
    75061 ttatgctgct tggcagaaat ttgctagacc acgctttgat aatatgatta gttttaatag
    75121 taccaacgat ttattaacat ggttacaaga tccattcatc aaagatatgc caggaaaacg
    75181 cgttaacatt aatgatggtg tgcccaggtt agatacttta atagacagcc atgtaggtta
    75241 taaaaggaag ttaaatagaa aagataacac atacgatact gtaccactaa tcaaaataaa
    75301 gtcggtaaaa gatacagaaa ttaaaaatgg aaaaaagtaa aaaagactat taacataaca
    75361 ttagatatgg atgggcgaat tccaataaat gtttggacag gagattcgat tgcacgttct
    75421 ggaagaggaa ctttaattaa acttaattta gaaaatcttg atgcgttgag taaactgatt
    75481 actggtgaaa caagtggtat gttagcagaa tgcgtaatct ttttaaatga aagttttaac
    75541 atctcagaaa atgaaaataa aaattttgca gatagaaaga aacaattatc agaaggattt
    75601 aaggataaga ttaacttatt tcagttagaa gaaatggaaa gaactttaat tagtaaaata
    75661 aactcacttg aagaagttgc agatgaaaca atagaaagta ttagtgctgt taaacactta
    75721 ttacctgatt ttgcattgga tgcattaaaa gaaagaatta atgagttgtt taaaggtata
    75781 aaatctttta tagaaaaagt gtatgatagt atagataatg aaattttaga aattttcaaa
    75841 aatatagatc acgacttcag agatggagta tctgaagaaa tgatgaaaca tttgaaagta
    75901 gtgaaacaga atatagacca aataaaaaat caaaatgata tttatggtag gcaaattgca
    75961 gatattagaa gtattatgaa acaacaagat gcaacaattt tagatggaaa ttttcaaatt
    76021 aattgtagcg gcgaaaatat ggtacagggt ctagttatac cttctaatta tttaggaaga
    76081 aaaatgaaaa tattaaaaga ccatatcgat gatggtatta aaaaaatagc agactatgtt
    76141 caaggtatat atgatgaata tgcatcgaaa attgtcgatg taataaaata tttgattaat
    76201 acaattccca aaatacgtaa gaatttaaga catgcaattg aaatgttaaa tgtaaaaaag
    76261 aaagaatttt tgtccctgat tcctaatgta acttgtaatt atattaaaac taaattagaa
    76321 gaattagata atactttagg caaatgggag ccttttctta atgatttaaa agcagtgtca
    76381 ccaattttag ataaccattt agatgatatt gttaagaaca tgaagccttt gattgtacaa
    76441 atgatatttg aaccatcaca ttatgatgat atgtttattt caagaaaagc tttaacgcca
    76501 gtgttctcaa gcgttttata aagcttgtaa aaaatataag ggcaaaaaaa gggcggattt
    76561 aagctaactt ggaatgtttt cgagtttttg agttagttct ctatccattt tttcagttac
    76621 atgagtatat atgcgaatgg ttgttttttc atctacatgt cctacccttt tcataattgc
    76681 ttttaaagaa acattcattt ctactaataa agttatgtgt gtatgtctaa atgtgtgcgt
    76741 ggtaactttc ttattcatat ttaaagcttt tgtagttttc ttaagcacac cggcgatttg
    76801 attattacat aaaggattcc ctttttttgt tgtgaatatg aaccctctgt caacatagct
    76861 cgaattccat cttttcaaca ttttgttttc cagtattatc tttttaaaaa tttctacggt
    76921 tctagaattg atgctgatac ttctttttga acttatagtc tttgtagtgt ctttgtatcc
    76981 gaatccttcc tcgtatttaa tgcggtgaat tgtacctgtt atattgatag ttttgtttaa
    77041 taaatctata tctttttcct gcagtgcttg tagttctcct atgcgcatac cagttaaagc
    77101 ctgtacttct aagatgctgg caattaaaat gcgatttcgc ttgtgtaact tattatcatt
    77161 tagtatatga tcacgtatct gtaggacttg gttcatttct aaatagttgt acattttaga
    77221 ttcatctttt tcgatatcct ctattgtttt tcttctttta ggaattttga cattagttaa
    77281 caaatattca tttggataat tgtaaaattt aactgcatat ttaatagctc ctttcatatc
    77341 tccgagttga cgggttactt gattttgaga atagatatct gataatttat taataaatat
    77401 ctgcatatat tttgtatcta gtttgtttaa aagcaagttc tcagagctgt atcgtttaat
    77461 gtttctaatt cttattttta tattattaag agtagtcaac tttgaacctg atgtttttat
    77521 atgatattca agccattcat ctaatagcgc gtgaaaagtc aaagttttta attcgcttga
    77581 cgacttgttg ttcagttttt cttttatttt ttcttctaaa cgaaacattg cttctttttg
    77641 agattgtttt gtattcttgt tcaacacaac acttacgcgc ttccatttat ctgtgtatgg
    77701 atctttatac ttctcgtagt atctgtattt agtttcgtta tttttgtttt taaatttttc
    77761 aatccacatg tttatacctc ctgcaagaac gtatgttcta taaaatatta aaaaataata
    77821 agggtagtcg ggctacccgt aatttagtac taggtactaa atgtgttata ataaaataaa
    77881 aagtaggtga taagatgact caatttctag gggcgcttct tcttacagga gttttaggtt
    77941 acataccata taaatatcta acaatgatag gtttagttag tgaaaaaaac aaggttatca
    78001 atactcctgt attattgatt ttttctatt
//
LOCUS       DALAAR010000003        70165 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000003 DALAAR010000000
VERSION     DALAAR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 70165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.003"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(11..1372)
                     /locus_tag="O2458_000171"
     CDS             complement(11..1372)
                     /locus_tag="O2458_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3886062.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1720..2841
                     /locus_tag="O2458_000172"
     CDS             1720..2841
                     /locus_tag="O2458_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCZ3886063.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2890..4158
                     /gene="icd"
                     /locus_tag="O2458_000173"
     CDS             2890..4158
                     /gene="icd"
                     /locus_tag="O2458_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCZ3886064.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4548..5252
                     /locus_tag="O2458_000174"
     CDS             4548..5252
                     /locus_tag="O2458_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3886065.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5252..6913
                     /locus_tag="O2458_000175"
     CDS             5252..6913
                     /locus_tag="O2458_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCZ3886066.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            7412..8899
                     /locus_tag="O2458_000176"
     CDS             7412..8899
                     /locus_tag="O2458_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886067.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            9193..11823
                     /gene="polA"
                     /locus_tag="O2458_000177"
     CDS             9193..11823
                     /gene="polA"
                     /locus_tag="O2458_000177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCZ3886068.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            11839..12711
                     /gene="mutM"
                     /locus_tag="O2458_000178"
     CDS             11839..12711
                     /gene="mutM"
                     /locus_tag="O2458_000178"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCZ3886069.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            12727..13350
                     /locus_tag="O2458_000179"
     CDS             12727..13350
                     /locus_tag="O2458_000179"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCZ3886070.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            13520..14545
                     /gene="gap"
                     /locus_tag="O2458_000180"
     CDS             13520..14545
                     /gene="gap"
                     /locus_tag="O2458_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ3886071.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            14755..15225
                     /gene="nrdR"
                     /locus_tag="O2458_000181"
     CDS             14755..15225
                     /gene="nrdR"
                     /locus_tag="O2458_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCZ3886072.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            15226..16626
                     /locus_tag="O2458_000182"
     CDS             15226..16626
                     /locus_tag="O2458_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCZ3886073.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            16626..17546
                     /gene="dnaI"
                     /locus_tag="O2458_000183"
     CDS             16626..17546
                     /gene="dnaI"
                     /locus_tag="O2458_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCZ3886074.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            17639..17761
                     /locus_tag="O2458_000184"
     CDS             17639..17761
                     /locus_tag="O2458_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886075.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            17959..19896
                     /gene="thrS"
                     /locus_tag="O2458_000185"
     CDS             17959..19896
                     /gene="thrS"
                     /locus_tag="O2458_000185"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCZ3886076.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            20322..21815
                     /locus_tag="O2458_000186"
     CDS             20322..21815
                     /locus_tag="O2458_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3886077.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    21890..22015
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            22044..22571
                     /gene="infC"
                     /locus_tag="O2458_000187"
     CDS             22044..22571
                     /gene="infC"
                     /locus_tag="O2458_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCZ3886078.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            22600..22800
                     /gene="rpmI"
                     /locus_tag="O2458_000188"
     CDS             22600..22800
                     /gene="rpmI"
                     /locus_tag="O2458_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCZ3886079.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            22847..23203
                     /gene="rplT"
                     /locus_tag="O2458_000189"
     CDS             22847..23203
                     /gene="rplT"
                     /locus_tag="O2458_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCZ3886080.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            23345..23953
                     /locus_tag="O2458_000190"
     CDS             23345..23953
                     /locus_tag="O2458_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ3886081.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            23972..24901
                     /locus_tag="O2458_000191"
     CDS             23972..24901
                     /locus_tag="O2458_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886082.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            24906..25016
                     /locus_tag="O2458_000192"
     CDS             24906..25016
                     /locus_tag="O2458_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886083.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            25064..26365
                     /locus_tag="O2458_000193"
     CDS             25064..26365
                     /locus_tag="O2458_000193"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCZ3886084.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            26516..27778
                     /gene="clpX"
                     /locus_tag="O2458_000194"
     CDS             26516..27778
                     /gene="clpX"
                     /locus_tag="O2458_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCZ3886085.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            27932..28522
                     /locus_tag="O2458_000195"
     CDS             27932..28522
                     /locus_tag="O2458_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCZ3886086.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            28739..30085
                     /locus_tag="O2458_000196"
     CDS             28739..30085
                     /locus_tag="O2458_000196"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCZ3886087.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            30107..30922
                     /gene="ccsA"
                     /locus_tag="O2458_000197"
     CDS             30107..30922
                     /gene="ccsA"
                     /locus_tag="O2458_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCZ3886088.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            30964..31890
                     /gene="hemC"
                     /locus_tag="O2458_000198"
     CDS             30964..31890
                     /gene="hemC"
                     /locus_tag="O2458_000198"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCZ3886089.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYIM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            31912..32580
                     /locus_tag="O2458_000199"
     CDS             31912..32580
                     /locus_tag="O2458_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCZ3886090.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            32583..33557
                     /gene="hemB"
                     /locus_tag="O2458_000200"
     CDS             32583..33557
                     /gene="hemB"
                     /locus_tag="O2458_000200"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCZ3886091.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            33605..34891
                     /gene="hemL"
                     /locus_tag="O2458_000201"
     CDS             33605..34891
                     /gene="hemL"
                     /locus_tag="O2458_000201"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ3886092.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            34952..35053
                     /locus_tag="O2458_000202"
     CDS             34952..35053
                     /locus_tag="O2458_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886093.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            35087..36160
                     /locus_tag="O2458_000203"
     CDS             35087..36160
                     /locus_tag="O2458_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCZ3886094.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(36383..36943)
                     /locus_tag="O2458_000204"
     CDS             complement(36383..36943)
                     /locus_tag="O2458_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCZ3886095.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    37001..37302
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            37346..39976
                     /locus_tag="O2458_000205"
     CDS             37346..39976
                     /locus_tag="O2458_000205"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCZ3886096.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            39989..41260
                     /locus_tag="O2458_000206"
     CDS             39989..41260
                     /locus_tag="O2458_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCZ3886097.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            41530..42237
                     /locus_tag="O2458_000207"
     CDS             41530..42237
                     /locus_tag="O2458_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCZ3886098.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            42234..42920
                     /gene="radC"
                     /locus_tag="O2458_000208"
     CDS             42234..42920
                     /gene="radC"
                     /locus_tag="O2458_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCZ3886099.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            42988..43272
                     /locus_tag="O2458_000209"
     CDS             42988..43272
                     /locus_tag="O2458_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886100.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(43389..43586)
                     /locus_tag="O2458_000210"
     CDS             complement(43389..43586)
                     /locus_tag="O2458_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886101.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            43746..44219
                     /locus_tag="O2458_000211"
     CDS             43746..44219
                     /locus_tag="O2458_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCZ3886102.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            44293..44412
                     /locus_tag="O2458_000212"
     CDS             44293..44412
                     /locus_tag="O2458_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886103.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            44612..45454
                     /gene="mreC"
                     /locus_tag="O2458_000213"
     CDS             44612..45454
                     /gene="mreC"
                     /locus_tag="O2458_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCZ3886104.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            45454..45984
                     /gene="mreD"
                     /locus_tag="O2458_000214"
     CDS             45454..45984
                     /gene="mreD"
                     /locus_tag="O2458_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCZ3886105.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    46011..46138
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            46151..46459
                     /gene="rplU"
                     /locus_tag="O2458_000215"
     CDS             46151..46459
                     /gene="rplU"
                     /locus_tag="O2458_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCZ3886106.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            46465..46785
                     /locus_tag="O2458_000216"
     CDS             46465..46785
                     /locus_tag="O2458_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCZ3886107.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            46797..47081
                     /gene="rpmA"
                     /locus_tag="O2458_000217"
     CDS             46797..47081
                     /gene="rpmA"
                     /locus_tag="O2458_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCZ3886108.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            47436..48728
                     /gene="obgE"
                     /locus_tag="O2458_000218"
     CDS             47436..48728
                     /gene="obgE"
                     /locus_tag="O2458_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCZ3886109.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            48741..49196
                     /locus_tag="O2458_000219"
     CDS             48741..49196
                     /locus_tag="O2458_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ3886110.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            49210..49812
                     /gene="ruvA"
                     /locus_tag="O2458_000220"
     CDS             49210..49812
                     /gene="ruvA"
                     /locus_tag="O2458_000220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCZ3886111.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            49844..50848
                     /gene="ruvB"
                     /locus_tag="O2458_000221"
     CDS             49844..50848
                     /gene="ruvB"
                     /locus_tag="O2458_000221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCZ3886112.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            50850..51875
                     /gene="queA"
                     /locus_tag="O2458_000222"
     CDS             50850..51875
                     /gene="queA"
                     /locus_tag="O2458_000222"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCZ3886113.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            51898..53037
                     /gene="tgt"
                     /locus_tag="O2458_000223"
     CDS             51898..53037
                     /gene="tgt"
                     /locus_tag="O2458_000223"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCZ3886114.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            53056..53316
                     /gene="yajC"
                     /locus_tag="O2458_000224"
     CDS             53056..53316
                     /gene="yajC"
                     /locus_tag="O2458_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCZ3886115.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            53591..55870
                     /gene="secDF"
                     /locus_tag="O2458_000225"
     CDS             53591..55870
                     /gene="secDF"
                     /locus_tag="O2458_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCZ3886116.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            56073..58346
                     /gene="recJ"
                     /locus_tag="O2458_000226"
     CDS             56073..58346
                     /gene="recJ"
                     /locus_tag="O2458_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCZ3886117.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            58368..58886
                     /locus_tag="O2458_000227"
     CDS             58368..58886
                     /locus_tag="O2458_000227"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCZ3886118.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            59314..61503
                     /locus_tag="O2458_000228"
     CDS             59314..61503
                     /locus_tag="O2458_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCZ3886119.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            61515..61967
                     /locus_tag="O2458_000229"
     CDS             61515..61967
                     /locus_tag="O2458_000229"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCZ3886120.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            61964..62839
                     /locus_tag="O2458_000230"
     CDS             61964..62839
                     /locus_tag="O2458_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ3886121.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            63300..64562
                     /locus_tag="O2458_000231"
     CDS             63300..64562
                     /locus_tag="O2458_000231"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCZ3886122.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            64578..66344
                     /gene="aspS"
                     /locus_tag="O2458_000232"
     CDS             64578..66344
                     /gene="aspS"
                     /locus_tag="O2458_000232"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCZ3886123.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            66422..66612
                     /gene="ssrS"
                     /locus_tag="O2458_000233"
     ncRNA           66422..66612
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O2458_000233"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            66677..66805
                     /locus_tag="O2458_000234"
     CDS             66677..66805
                     /locus_tag="O2458_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886124.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            66805..67578
                     /locus_tag="O2458_000235"
     CDS             66805..67578
                     /locus_tag="O2458_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCZ3886125.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(67739..69013)
                     /locus_tag="O2458_000236"
     CDS             complement(67739..69013)
                     /locus_tag="O2458_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCZ3886126.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            69098..69520
                     /locus_tag="O2458_000237"
     CDS             69098..69520
                     /locus_tag="O2458_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ3886127.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            69620..69802
                     /locus_tag="O2458_000238"
     CDS             69620..69802
                     /locus_tag="O2458_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ3886128.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            69842..69988
                     /locus_tag="O2458_000239"
     CDS             69842..69988
                     /locus_tag="O2458_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886129.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT        25219 a         9603 c        13362 g        21981 t
ORIGIN      
        1 aaacttgttg ttatttattt gatttagcag cgatacgttt atatcttaag tacataaatg
       61 ctaaaagtat aaaccaaatc ggaataaaat aaattgcacg tcttgtatca acattaataa
      121 ataataaccc gaacacaaaa atgaagaata caaatattaa gtagcccata tatttgccac
      181 ctaatagttt gtacgtagca tttttatgta gatctgggtt tttacgacta taattgatat
      241 atgcaatgat aatcagaccc catacaacta aaaataacac tgtagagatg gtagtcacat
      301 acgtaaatac ttttgtcgca tctgggaaaa tatagtttag taatgctgcc acaagtaata
      361 atgctgatga agcaaagatt gcaacatgtg gaacgccata tttattcgtc ttagaaaagt
      421 tcggaggtgc ttgttgttga cttgataaac cgaaaagcat acggctattt gagaatatac
      481 cactgttaca tgatgaagca gcagcggtta atactacaaa attaatcaag cccgcagcaa
      541 acggaattcc gatcaatgcg aataatttta cgaatggact gttatcagga tcaacttgct
      601 gccaagggat aatagacatg ataaccgcta acgccccaac gtaaaatatt aaaattctaa
      661 tgggtacact gttaattgct tttggaattg ttttaactgg atcttttgtt tccccagctg
      721 taacaccaat catttcaatt cctacaaatg agaatagtgc catttggaaa gacataaaga
      781 atccagaagc acctttagcg aatatgccgt gttcatatag atttgtgaaa ctggcatgcc
      841 cgaattgagt tttaaatgca aatagaatca tgacgaaacc aactactatt aaaccaataa
      901 ttgtcgctat tttaatgata gagaaccaaa attctaattc tccgaaaagt cttgcgctaa
      961 gtaggttgaa tgacattaat aacagtacac aaaatagtgc acttatccag tttggaattt
     1021 ctgggaacca aaagctaaca tattttgcca cagccgttac ttcagccata cctgtaataa
     1081 tccaacagaa ccagtatgtc catccggtaa caaatcctgc aaaaggccca atatatgtat
     1141 tggttacatc tgcgaaagat ttaaattcag tattctgtat aatgatttct cctaaacctc
     1201 gcataaacat aaataacata aatcctataa tgatgtatgt taatagaatt gaagggccgg
     1261 ttaatgcaat cgtttgacca gcacctaaga ataagcctgt accaattgca ccgccaattg
     1321 caattaattg tatgtggcga ttgctcagtt ccctttgtaa tttttcagcc ataatacatc
     1381 tcccttaaat atagatatgt ttattatgca cttatattga gatatataca attattttcg
     1441 gtaaaaatgt gtaaaattcc atgttaatat actttggttt ttataatcat atataataac
     1501 caattgaaaa tttaattcta ttgtaaaatt catggattat tcacatcttg aaaaagcttt
     1561 aatggtgcta tttgtggcta ttctgtgaca tttacataga tttacaaaaa aattgttgca
     1621 catataatgc cagtttttat atttcacaaa cgaaatgcgt ttagctataa tattagttga
     1681 aagccatttc ataaagaaac agtaaagggg aaatttatca tggcagaatt acaaagaggt
     1741 ttagaagggg ttatcgcagc ggagactaaa ataagttcaa ttattgaaag tcaattgact
     1801 tatgccggct atgatattga tgatctagct gaaaatgcgc aatttgaaga agttattttc
     1861 ctattatgga actatagatt gccaaacgaa gaagagcttg ctcatctaaa agggaaatta
     1921 aatcaataca tgacattaaa tcctcgtgtg tatacacatt ttgaggagta tgttacagat
     1981 cacgtgcatc caatgacagc attacgtacg tcattatcat atattgcaca tttcgatcct
     2041 gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag caatgcgtat acaggctaaa
     2101 gtagcatcat tagttacagc gtttgctcga gtaagacaag ataaagaacc acttaagcct
     2161 aatcctgact taagttatgc ggcaaacttc ctatatatgt tacgtgggga attaccaaca
     2221 gatatagaag tagaagcctt caataaagca cttattttac acgctgatca tgagttgaac
     2281 gcatctgcat ttacggcacg ttgtgcggta tcatcattgt cagatatgta ctcaggtatt
     2341 gtagcagccg taggttctct gaaagggcca ttacatggtg gtgcaaacga acaagttatg
     2401 acgatgttat ctgagattgg gtcaattgaa aatgttgatg cttacttaga tgaaaaattt
     2461 gctaataaag ataaagtaat gggcttcggt catcgtgtat ataaagatgg tgatcctaga
     2521 gcgaaatatt taagagaaat gagccgtcaa attacgaaag acgctggtcg tgaagaatta
     2581 tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag aaaaaggatt aattcctaat
     2641 gttgattttt atagtgcgag tgtttatcac tgtatggaaa tacctcatga cttattcacg
     2701 ccaatctttg ctgtaagtcg ttctgcagga tggattgctc atattttaga acaatataaa
     2761 gataatagaa ttatgcgtcc tagagcgaaa tatattggcg aaacgaatcg taagtatatc
     2821 ccgcttgaag aaagaaaata atcaatacaa attaaaaatg aagatgtaaa atttggaggt
     2881 aaaataacta tgactgcaga aaaaattact caaggaactg aaggattaaa cgtacctaat
     2941 gaaccaatta tcccatttat tatcggtgat ggaattggac cggatatttg gaaggcagca
     3001 agccgagtta tagatgctgc tgttgagaaa gcctataatg gcgaaaaacg cattgaatgg
     3061 aaagaagtgc tagctggcca aaaagcattt gatacaactg gtgaatggtt acctcaagaa
     3121 acacttgata caattaaaga atatttaatt gctgttaaag gacctttaac aacaccaatt
     3181 ggtggtggta ttagatcatt aaatgtggct ttacgccaag aattagattt atttacttgc
     3241 ttaagaccgg tacgttggtt taaaggagta ccatcacctg ttaaacgtcc acaagatgtt
     3301 gatatggtta ttttccgtga aaatactgaa gacatttatg ctggtattga atttaaagaa
     3361 ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa acgaaatggg tgcgacaaac
     3421 attcgattcc cagaaacttc aggtattggt attaaaccag tttctaaaga aggaactgag
     3481 cgattagtta gagcagctat acaatatgct atcgataata accgtaaatc agttacttta
     3541 gttcataaag gtaatattat gaaatttaca gaaggctcat ttaagcagtg gggttacgat
     3601 ttagcattat ctgaatttgg tgatcaagta ttcacttggc aacaatatga cgaaattgtt
     3661 gaaaatgaag gcagagatgc tgctaatgct gctcaagaaa aagctgaaaa agaaggcaag
     3721 attatcatta aagattctat tgctgacatt ttcttacaac aaattttaac tcgtccagct
     3781 gagcatgatg ttgtagcaac tatgaacttg aatggtgact atatttcaga tgctttagct
     3841 gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca ttaattatga aacaggtcat
     3901 gctatttttg aagcaacaca tggtacagct ccaaaatatg caggtttaaa taaagtgaat
     3961 ccatcttcag taattttaag ttctgtatta atgttagaac atttaggatg gcaagaagcg
     4021 gcagataaga ttacagattc aattgaagat acaattgctt caaaagttgt tacttatgac
     4081 tttgcccgtt taatggatgg tgctgaagaa gtttctacat cagcatttgc agatgaattg
     4141 attaaaaatt taaaataagc aaaataaaat taggtgagtc tagcaacttt aattgctagg
     4201 ttcgcctttt taaatgaatt gaagttggca acaggataga aataataaag agtcaccaat
     4261 gattgattat gtagtgtttc ttacacatta gacaaagcga atatgtacat aagcttagga
     4321 ataaatgagt aaaactcatg cataggtagt gggatagaaa tgctattttt acaaaattta
     4381 tttcgttgtc ccagagtaag tcatgcaaat gatagaagga aatataagaa gtattttatt
     4441 tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg taggatgtta tttttataaa
     4501 tagttcggtt ataatgtaaa tataattata agttagggag gcataccatg tcgcaaaaag
     4561 tgttggtagt agatgacgaa cattcaatcg taacattact taaatataac ttagaaacag
     4621 ctggttatga agttgttgtc gcatttgatg gtgatgaggc tttagaaaag gtagaaagtg
     4681 aacagccaga tttaattatt ttagatgtta tgctacctaa aaaagatggc attgacgtat
     4741 gtaagactgt aagaacaaat aaaaatttag tccctatatt aatgttaacg gcgaaaaatg
     4801 atgaatttga tcgggtatta ggtttagaat taggtgccga tgactatatg actaagcctt
     4861 tttcacctag agaagttgtt gcccgtgtga aagccatttt aagacgttct caatttgtaa
     4921 aagaagtaga agagattgac gatgatatta ccatcggatc gatacgtatt agacccgatt
     4981 attttgaagt atataagcat aatgagttat tggagctaac accgaaagaa tttgaactct
     5041 tgctttattt gatagaaaga caaggcagag tcattacgag agagcatatg ttaaatacgg
     5101 tatggaatta tgaatttgca ggagactcaa gaattgttga tgtacatatc agtcatttaa
     5161 gagataaatt agaagataat cctaagaagc cacaacttat taaaactgtg cgtggcttag
     5221 gctataaatt ggaaagacct aaagaacaat gatgaagttt caccaccgct taatgttatt
     5281 gataagcagt atattaatta tcagttttgt tacattaggc attattattc acgcttccat
     5341 cacatatgtt ttatcagaaa aacacgaaca agctctagca acagatgcaa gatcatatat
     5401 ttatttagtc caagataatg agattgataa agttaaaaaa attgtaaaac agcaaaatat
     5461 agatttattg ataactaagc atgataaaaa ggtgtttagt agtggcaaac tgaaagctat
     5521 cccaaatagt gatagctata ttcaaaaaag ggacgttttt atttttaata agaaggttaa
     5581 cggttatcat gtatggatta aaggttacaa taacgatatt acagaaatgc attggacgtt
     5641 gtggaaatat cttattttga catgtttagt tgtactcatt tgtctgtatt ttgcaagccg
     5701 tagttttaag cgtacattaa tccgtcccat tcaagaagtt acttatgcaa cacaattgtt
     5761 ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat gttgtagaaa ctaaagcgct
     5821 ttttgtgtct accaatgatt tagcacgacg ccttcaaaag cttaataatg agcaaaaaat
     5881 tcaatccaac cgtttaaaaa caacgattga aaatattcca agtgctattt taatgataga
     5941 tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat gaacagttca acatctcgca
     6001 taatattgaa caagtaggct atcacggcta tgttaatact gaaattgagc aattaatttt
     6061 agaaagtttt aaagttgaaa aacctattta tgaacaattg gaagttgcaa taaaccaagt
     6121 ccatgccaag tactttgata tttcatgcgt tccgatttta actaggtctc aaaaaagttt
     6181 gcaagggatt ttagttgtga tgcatgatat aacaaatctg aaacaacttg aaaatctaag
     6241 acgtgaattt gtagcaaatg tttcacatga attaaaaaca cctattactt caattaaggg
     6301 gtttgctgaa acgcttattg atggtgcaaa aaatgatgcg gaatcattag acatgttttt
     6361 aaacataatt ttaaaagaat caaatcgaat agaatcttta gtaacagatc tgttagattt
     6421 atcgcatatt gagcaacata cagagttaga tacagactat atgaatttat cagatttaac
     6481 gcggcgtatt attgataata tgatgactca agccaatcaa aaaaatattt ccattcatac
     6541 tgatattgaa aaagatgtca ttgttaaagc acaagaaagt aaaattgctc aagttatcac
     6601 gaatttgtta acgaatgcaa ttaactattc ttatgaagat ggagatatta acgttcgtgt
     6661 gtatcgagat gactttcgtg tcattttcga agtacaagat tttggtatag gtattaaatt
     6721 agaagaccaa caacgtattt ttgaaagatt ttatagagta gataaagcac gaagtagaga
     6781 ttctggtggg acaggtcttg gattatcaat tacaaagcat atagtagagg cccaccaagg
     6841 caatattgaa gtgaatagtc aagttggcaa aggctcaacg ttcaaagtta ttctaaaaga
     6901 ttataaagaa taaaaattaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg
     6961 cttttttatt tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaataaa
     7021 ttcggttgtc aattttgtca tttgtatatg tcgatttgta agttataagt aatagattgc
     7081 tgtgaatcaa gcaattttat aatatattta ttataataaa gaaattttag taacgcgctt
     7141 gaaatagatg cttaattaaa aagtaataag ttttatttga acggaaataa atcttaattt
     7201 aaacacttga atagacaagt gtaatcattc gtcatagaaa aagacgaggg gcgatttaga
     7261 taaattgttg aaagtaatat taaatacctt gtttatctaa tttgattgag gaaacatgca
     7321 taggtgaaaa ttagaaatac attaaaaata ttgaattaat ttttcgacct acaagttgag
     7381 gaaaatttaa ctagatttga ggattagcaa agtgaaaaca attcaattac acaagacaac
     7441 tttgatatta attgcgatat tattgtttta tacttttatg ggtatcttac taccactcat
     7501 gcacgatgat ttacaatggt ttagcaatta taatactgat attttaaaag taggatttgc
     7561 atcacttaat ggtcgctata tcggaaatat ctttgaaatt atagctgtac atgtaagctg
     7621 gttacgttgg ctttcatatg gcctcattag tatgggcatc atttggatga ttatgcacat
     7681 tacacgttgt aaagcgtgga caagctatta tttgttagca ttttctttaa tgttaatttt
     7741 accaagtgct atttatgcag atacatatgg ctggtttgca ggattttata attatgcgac
     7801 atcaacacta atttcactct ttattattta ttattgtatt aatgcaatta tatataaaga
     7861 aaagcagccg gtaagtgtta ctgtactatt ctatgtttta tgcttcttcg gtcaattatt
     7921 tatggagaat gtgacattat tccactgtat cattttaatt ttagcttttc tgtatgaatt
     7981 tactgcaaat cgaacactta attataaatt attgttttca tttatgattg ctactatagg
     8041 tacaatcatt atgttttcca atccaaatta tcgcaaaatt ttatttgaag gatcagaata
     8101 tcaacaagtt tcaaataatc aaggtatctt ttcaaaattt gcagaaatga tatcaacgtc
     8161 acttccatat ggtgtcattt tcagtcagat tataatttta agtatgattg cagcgcttat
     8221 tatttatcta ctgcttagaa gtgatcggta tgtacattta acaatattaa agcgtcgtat
     8281 cattatgata ggatttatca ctttaccgct ttattattta ctattttaca atcagttttt
     8341 attaaataaa aatacagata ttggattggt tggttttgtg aacgtaattg tatgtggtta
     8401 ctttgcgttt tcgatatttg taggtattta tttgtctatt aatgatcgta aaacacaaat
     8461 cactttatat tccttactta ttgcaatatg tgtttcggcg tcgacactag tcattgttac
     8521 tccaatagaa ccaggtaatt ttttgattgt ttatacgata catgtaatca ttttaattat
     8581 tttattaaaa gaatttcgga aatataagtc aatcaatgtt aattttatta aaggtgcatc
     8641 actagtttta gctgtgatat atcttagtgc atttatttat gttcattatg aacatgaaaa
     8701 aagagttcag ttattaaaac aacaaattaa agatcatcca cgacaagagg tttatacagt
     8761 ggaatcattg ccgtttgaac attatatgca tcatccgtca ccaatcaata aaaattacca
     8821 agagtggttt aataattatg aagaattacc gaaaatgacc aaagttaaat atattccatt
     8881 tggttcagac gattcataac ttattcttac ctataaaaat ttaaaatagc agcataaaaa
     8941 tattaaaaag tagacataat catgaaatga taatgtgcat ctcaaaagtt ggacttttaa
     9001 cctgacttta tggagtgctc atcaagaaca atcatgatta tgtctttttt attttttagc
     9061 taagtctata taggcacttg ttttatttga aattaggtcg tgtataaata ctctatgaga
     9121 tagtttcttt aattacgaca tcacagtcga atatgctaaa atatatataa attagttctg
     9181 gaggttaaaa ttgtgaataa attagtatta atcgatggta atagcttaag ttttagagct
     9241 ttttatgcat tacctttgtt atcaaacaaa gcaggcattc ataccaatgc agtatatggt
     9301 tttgcaatgt tactagagaa gattttaaaa gaagaaaagc caaatcattt tttagtagcg
     9361 tttgatgcag gtaaaacgac tttcagacat gaaaaatata gtgaatataa aggtggacgc
     9421 cagaaaacgc cgcctgaact aagtgagcaa tttccttata ttcgccaatt attagatgct
     9481 tatcatatta aacgttatga attagataac tatgaggcag atgatattat cggaacttta
     9541 agtaaagaag cagataaagc gggctttcag acaattatta ttacgggaga ccgagattta
     9601 acacaacttg caacggataa tgtaaccatt tattacacta aaaaaggtgt tactgatgtt
     9661 gatcattata cacctgactt tattgcggaa aaatacaacg ggttaacacc taatcaaatt
     9721 attgatatga aaggattaat gggagatact tctgataata taccaggtgt tgcaggtgtt
     9781 ggagagaaaa cagcaataaa attgctaaac caatttgaca cagtagaagg tgtctatgaa
     9841 catttagatg aaatttcagg taaaaagtta aaagaaaaac ttcaaaatag caaagaagat
     9901 gcattaatga gtaaagaatt agcaacgatt aatgtagata gtccgattga agtaaaactt
     9961 gaagatacgt taatgactca tcaagatgaa caacaagaaa aaatcgaatt gtttaagaag
    10021 ttagaattca aacaattgtt ggctgacatt gatcaatcag caagcgttga agatgcaata
    10081 gaaaagacat ttgaaattga aacgtctttt gataatattg attttacttc attgaaagaa
    10141 gcggccatcc atttcgaatt agacggtggt aactatttgc gaaataatat tttaaaattc
    10201 tctttattta caggtgagaa acatattgta attaatgcgg atgacataaa taattatgtc
    10261 gaactagttt catggttaga aaatccgaat tcgaaaaaag tcgtatatga tgctaaaaaa
    10321 acatatgtag catcacatag attggggatt gatattcaaa atatttcttt cgatattatg
    10381 ttggccagtt atataattga tccatctcgt acgattagtg atgttcaatc agttgtttca
    10441 ttgtacggtc aaagttttgt gaaagacgat gtgagtatat atggaaaagg taagaaattt
    10501 aaggtacctg aagatgatgt tttaaatcca tatgttgctt ctattactga tgcaatttac
    10561 tttgcgaaac caaatatgga taaacaatta gaagaataca atcaggtaga actcttggct
    10621 gatttagagc taccgctagc taagatttta agtgaaatgg aagaaattgg tatatttaca
    10681 gatgttcatg atttagaaga aatggaaaaa gaaattcaag aaaaattaga cgtcttgatt
    10741 cgaaatatcc atgatgcagc tggtgaagat tttaatataa attctcctaa gcaattaggt
    10801 gttgtattgt ttgagacatt acaattacct gttattaaaa agacgaaaac aggatattct
    10861 actgctgtag atgtcttaga gcaattgcaa ggtgaacatc ctatcattga ttatatttta
    10921 gaatatcgtc aattatctaa gttacagtct acttatgttg aaggattgca aaaggtaatt
    10981 agtgatgatc aacgtatcca tacacgtttt aatcaaactt tagcgcaaac tggacgttta
    11041 tcaagtgtag atcctaattt acaaaatatt ccggttagac ttgaagaagg gcgtaaaata
    11101 agaaaagcct ttaaaccaac ttcaaaagat agcgttatat tatcagcaga ttattctcaa
    11161 attgaattgc gtgtattagc acacattaca caagatgaga gtatgaaaga agcatttatc
    11221 aacggcgatg atattcatac agcaactgct atgaaagtat ttggtgtaga agctgatcaa
    11281 gtcgatagtt taatgcgtcg tcaagcaaaa gcagttaact ttggaattgt ttatgggata
    11341 agtgattatg gtttaagtca aagtttaggt attactcgta aaaaagcaaa agcattcatt
    11401 gatgattatt tagctagttt cccaggtgta aaacaatata tgtctgatat tgtaaaagat
    11461 gccaaagctt taggttacgt ggaaacattg ctacatcgtc gacgctatat tcctgatatt
    11521 acgagtcgta actttaattt acgcggcttt gctgaacgta ctgctatgaa tacgccaata
    11581 cagggcagtg ctgcagatat cattaaactg gcaatggtta aatttgctca aaaaatgaaa
    11641 gagacaacat atcaagctaa actattatta caagtacacg atgaattaat ttttgaagta
    11701 cctaagtcag aagtagattc atttagtgaa tttgtagaag agataatgga aaatgcattg
    11761 caattagatg ttccattaaa agtagactca agttatggtg caacctggta tgatgcaaaa
    11821 taaagtgagg ttaatggtat gcccgaatta ccagaagtag aacatgtaaa aagaggaatt
    11881 gaaccctacg ttataaatca aaaaattgaa cacgttatat tttcggataa agtaatcgaa
    11941 ggtaaagctc aaggcaagga aacaattata aaaggaattg aattagatac ttttaaaact
    12001 ttatcggaag gctataccat tacaaatgta gaacgaagaa gtaaatatat cgtttttcag
    12061 ctagataata aacgagaaca acgtacatta attagtcatt taggtatggc gggcggtttt
    12121 ttcattgtag atgaacttga agatattatg atacctaatt atcgtaagca ttggcatgtt
    12181 atttttgagt tgtcaaatga caaaaagtta atttattcag atatacgtcg ttttggagaa
    12241 attagaaatg ttgcgtctgt agcatcttat ccttcatttt tagaaatagc gcctgaacct
    12301 ttttcaaatg aagcattgac gtattattta aatcgaattc atcaacagtc taataagaat
    12361 aaaccaatca aacaagtgat tttggatcat aaagtaatcg caggttgtgg aaatatttac
    12421 gcatgtgaag cgttgtttcg tgcaggtgtt ttaccagata aaaaagtaaa agacttaaca
    12481 caccaacaac aagaaatggt tttttattat gttagagaag tactagaaga aggtattaaa
    12541 tatggcggta caagtatttc agattatcgt catgctgatg gaaaaactgg tgagatgcaa
    12601 ttgcatctta atgtatataa gcagccggta tgcaaagtgt gtggaagtca aattgaaaca
    12661 aaaatcatcg cgacaagaaa tagtcattat tgtccagtat gtcaaaaata aagaaagagt
    12721 gaaattatgc cgaaagttat tggtctaaca ggtggaatcg cctcaggaaa atcaacagta
    12781 tcagaactct tatccgtatt cggttttaaa gtagtagatg ctgataaagc agccagggaa
    12841 gctgttaaaa aggggagtaa aggtttagct caagtacgag aagtctttgg tgatgaagca
    12901 attgatgaaa atggtgagat gaatcgtcgt tatatgggtg atctagtgtt taatcatcca
    12961 gaaaaacgct tagaattaaa tgctatcata catcctatcg tgcgagatat tatggaagaa
    13021 gaaaagcaag aatatttaaa acaaggatat aatgtaatca tggatattcc attattattt
    13081 gaaaatgaat tggaaaatac agtagacgaa gtgtgggttg tatacacttc tgaaagtata
    13141 caaatggatc gtttaatgca acgtaataat ttgtcattag aagatgcgaa agcacgtgtc
    13201 tatagccaaa tttctattga taaaaaaagc cgaatggccg atcatgttat cgataattta
    13261 ggggataaac ttgaattaaa acaaaacctt gagagattgt tagaagaaga aggttatatt
    13321 gaaaagccga attacggaga agaagattaa tattacacta taaataagtc attactttac
    13381 gtacgcgttg atgtatgtaa gtaatgacta ttttttataa aaaagataaa taaatcaacg
    13441 gaaaacgctt tcaaatttca tataatatgc tatactaatt ctataaagta taacacataa
    13501 agatcaaggg gtgcttttaa tgtcaacgaa tattgcaatt aatggtatgg gtagaattgg
    13561 aagaatggta ttacgtattg cattacaaaa taaaaattta aatgtagtag cgataaatgc
    13621 tagttatcca cccgaaacaa ttgcacattt aatcaattac gatacgacac atggaaaata
    13681 taatctaaaa gttgaaccga ttgaaaatgg attgcaagtt ggagatcata aaattaaatt
    13741 ggttgctgat cgcaatcctg aaaacttgcc atggaaagaa ttagatatcg atattgctat
    13801 agatgcaact ggtaaattta atcatggtga taaagccatc gcacatatta aagcaggtgc
    13861 caaaaaagtt ttgttaactg gtccttcaaa aggtggacat gttcaaatgg tagttaaagg
    13921 cgtaaatgat aaccaattag atatagaagc atttgacatt tttagtaatg cttcatgtac
    13981 tactaattgc attggtccag ttgcaaaagt tttaaataat cagtttggaa tagttaatgg
    14041 tttaatgact actgttcacg ctattacaaa tgaccaaaaa aatattgata atccacataa
    14101 agatttaaga cgtgcacgtt catgtaatga aagcattatt cctacttcta ctggtgcggc
    14161 gaaagcttta aaagaagtat taccagaatt agaaggtaaa ttacacggca tggcattacg
    14221 tgtaccaaca aagaatgtat cgctcgttga tttagttgtt gatttagaaa aagaagtaac
    14281 tgcagaagaa gtaaaccaag cttttgaaaa tgcaggttta gaaggtatca tagaagtcga
    14341 acatcaacca ctagtgtctg ttgattttaa tactaatcca aattcagcta ttattgatgc
    14401 aaaatcaaca atggtcatgt cagggaataa agtaaaagtt attgcttggt atgataatga
    14461 atggggttat tcaaatagag ttgtagatgt tgctgagcaa attggagcac ttttgacatc
    14521 aaaagaaact gtaagtgcaa gttaatacaa ttaatttata gcctaactct gacttcggag
    14581 ttaggttttt tatttttaat acaataatat tgtcatgcaa attacttaat aagtaacgat
    14641 ataatgtttt ttattgtttc attatttggt taaatgaatt aaattgaggg attctttttt
    14701 catctctaga tttatagtat aattaggcat aatcaaaata aagagggtga tgtaatgaaa
    14761 tgcccgaaat gtaattctac acaatctaaa gttgtagatt caaggcatgc cgatgaatta
    14821 aatgccattc gaagacgaag agaatgtgaa aattgtggaa cacgtttcac tacatttgaa
    14881 catatcgaag ttagtcagct tatagttgtg aaaaaagatg gcacaagaga gcagttttca
    14941 agagaaaaga tacttaatgg acttgtgcgt tcttgtgaga aacgaccagt tagatatcaa
    15001 caacttgaag acataactaa caaggttgaa tggcaattac gagatgaagg tcatacggaa
    15061 gtgtcttcac gagatatagg tgaacacgtt atgaacttgt taatgcatgt tgatcaagtt
    15121 tcatatgtta gatttgcatc agtctataaa gaatttaaag atgttgatca attgttagca
    15181 tcgatgcaag ggattttaag cgaaaacaaa cggagtgatg cttaaatggg acgacaagcc
    15241 ttcgaattcg gcttaagacc aaaggatcaa ttcaaagtta tgcaacactt tgatttaaat
    15301 acgaatcatt tagaggtatt gaatcgactg tatacaccgc taattggaac gcaagcagtg
    15361 ggtttatatc attttatgac tcaattcgtt aaagaaagtc ataatgaaac attgattctt
    15421 tctcattata tttttatgaa tgaacttaaa attaatttac tagagtttag acaacaaatg
    15481 gacttacttg aagcgattgg attgttaaag gcgtttgtca aacatgatga acaagaaaca
    15541 caatttgttt accaattaat tcaaccgcct tcagcacatt tattttttaa tgatccaatg
    15601 ttgtcaatct ttttatatag tgaagtcgag catcgtcgat ttcatgagtt gaagaaatac
    15661 tttgagtatc aacaaattga tttgagtgaa tttaaacagg taacaagaca atttactgat
    15721 gtttttaaag tgccgtctac aaaaatagat atcgatacaa gtgatattcc gattaatgaa
    15781 ccgtaccaag gtatcgattt atcaaatgaa tcttttgact ttgaaatgtt gagacaaatg
    15841 ttaggtaaac attttattag tcaagacatt gttaccaaag atgcaaagcg tttaattaca
    15901 caactcgcga ctttatatgg tttaacagca gatggcatga agcatgttat tttaaattca
    15961 attacaagtg gacaacagtt atcctttgaa gaaatgcgta aacaagcacg ctcgtattac
    16021 ttaatggaac acgaaaatca aatgcctaaa ttacaggtga aatctcctgc tacatcatct
    16081 tcggcgggca aatcttcaga agtgaatcca aagccacaaa gtgatgaatg gtttgagtta
    16141 cttgaacaaa cgagtccaat tgatatgtta gcttcttggt ctgaatcaga accaactata
    16201 tcgcaaaaga caatggttga agaattaatt gaacgtgaaa aaatgtcttt tggcgtcatt
    16261 aatattttgt tgcaatttgt catgttaaaa gaagatatga aacttcctaa agcgtatatt
    16321 ttggaaatag catcaaattg gaagaaaaaa ggtattaaga cagctaaaga agcatataat
    16381 tacgctaaaa aagttaatca acctaaaaat gagggttcta gcggaaatta ccaaaagcgt
    16441 ggttcatact atgggcagcg taatcgtatt tcaaaagaaa aaacacctaa atggttagaa
    16501 aatagagata aacctagtga agaagattcg gctaaagata atagcgtaga tgatcaacaa
    16561 ttagagcaag atcgacaagc atttctagat aaattatcta aaaaatggga ggaggacagt
    16621 caataatgaa gcaatttaaa agtataatta acacgtcgca ggactttgaa aaaagaatag
    16681 aaaagataaa aaaagaagta atcaatgacc cagatgttaa gcaatttttg gaagcgcatc
    16741 gagctgaatt aacgaatgct atgattgatg aagacttaaa tgtgttacaa gagtataaag
    16801 atcaacaaaa acattatgac ggtcataaat ttgctgattg tccaaatttc gtaaaggggc
    16861 atgtgcctga gttatatgtt gataataacc gaattaaaat acgctattta caatgcccat
    16921 gtaaaatcaa gtacgacgaa gaacgctttg aagctgagct aattacatct catcatatgc
    16981 aacgagatac tttaaatgcc aaattgaaag atatttatat gaatcatcga gaccgtcttg
    17041 atgtagctat ggcagcagat gatatttgta cagcaataac taatggggaa caagtgaaag
    17101 gcctttacct ttatggtcca tttgggacag gtaaatcttt tattctaggt gcaattgcga
    17161 atcagctcaa atctaagaag gtacgttcga caattattta tttaccggaa tttattagaa
    17221 cattaaaagg tggctttaaa gatggttctt ttgaaaagaa attacatcgc gtaagagaag
    17281 caaacatttt aatgcttgat gatattgggg ctgaagaagt gactccatgg gtgagagatg
    17341 aggtaattgg acctttgcta cattatcgaa tggttcatga attaccaaca ttctttagtt
    17401 ctaattttga ctatagtgaa ttggaacatc atttagcgat gactcgtgat ggtgaagaga
    17461 agactaaagc agcacgtatt attgaacgtg tcaaatcttt gtcaacacca tactttttat
    17521 caggagaaaa tttcagaaac aattgaattt taaaatgatt ggtgtataat gaatacaaat
    17581 ctaaatcgtt taaatgattg aagacaagat gatctaatca atattacaca gaaagccatt
    17641 gtttgatgag aatatggtta ataaattaga tgattactac ttcatttatg gtatttgtaa
    17701 tgaatacccg gatcaagacc gttatcttaa gcagagatat ttaatacata aattgtatta
    17761 gataactagg gtggtaccac gacgatctcg tcccttgtat aggggcggga ttttttgttt
    17821 ttttcagaca taaatgtttg ttggtgtcat aaattccctg tttattgtta ataggtttaa
    17881 tgttaaaacg atgattgttg ttcaattttt taacgaggtc agataaaagt atttataaag
    17941 caaataggag ggtttaacat ggaacaaatt aatattcaat ttccagatgg taataaaaag
    18001 gcgtttgata aaggtactac tactgaagat atagcacaat caattagtcc tggattacgt
    18061 aaaaaagctg ttgccggcaa atttaacggg caacttgtag atttaactaa accgcttgaa
    18121 actgatggat caattgaaat tgtgacacca ggtagtgaag aagcgttaga ggtattacgt
    18181 cattctactg cacatttaat ggcacacgcg attaaaaggt tatatggtaa tgttaaattt
    18241 ggtgtaggtc ctgtaataga aggtggattc tactatgact tcgacattga ccaaaacatc
    18301 tcatctgatg actttgaaca aattgaaaaa acaatgaaac aaatcgttaa cgaaaatatg
    18361 aaaatcgaac gaaaagtggt ttcacgagat gaagcgaaag agttattcag caatgatgaa
    18421 tacaaattag aattaatcga cgcgattcct gaagatgaaa atgtaacatt atatagtcaa
    18481 ggtgatttta ctgatttatg tcgtggagtt cacgttccat caacagctaa aattaaagag
    18541 tttaaactat tatctacagc aggtgcatac tggcgtggag atagtaacaa caaaatgtta
    18601 caacgtatat acggtactgc tttctttgat aaaaaagaat tgaaagcaca tttacaaatg
    18661 ttagaagagc gtaaagaacg tgatcatcgt aaaattggta aagagttaga actattcaca
    18721 aatagccaat tagttggtgc tggtttgcca ttatggttac ctaacggtgc aacaattaga
    18781 cgtgaaattg aacgttacat tgttgataaa gaagttagca tgggatatga ccacgtttat
    18841 acaccagtac ttgctaatgt tgatttatac aaaacatctg gtcactggga tcactatcaa
    18901 gaagatatgt tcccaccaat gcagttagat gaaactgaat ctatggtatt acgtccaatg
    18961 aactgtccac atcatatgat gatttatgcg aataaaccac attcatatcg tgaattacct
    19021 atccgtatcg ctgagctagg aacgatgcat agatatgaag caagtggtgc tgtatcagga
    19081 ttacaacgtg ttcgtggtat gactttaaat gattcacata tctttgttcg acctgatcaa
    19141 attaaagaag aattcaaacg cgttgtaaac atgattattg atgtgtataa agactttggt
    19201 ttcgaggatt atagctttag attaagttat agagaccctg aagataaaga aaagtacttt
    19261 gatgatgatg atatgtggaa taaagctgaa aatatgctta aagaggcagc ggatgagctt
    19321 ggcttatcgt acgaagaagc gattggtgaa gcggcattct atggtccgaa actagatgtt
    19381 caagttaaaa cagcgatggg taaagaagag acattatcaa cagcacaact tgatttctta
    19441 ttaccagaac gttttgattt aacttatatt ggtcaagatg gtgaacatca tcgtccagtt
    19501 gttattcatc gtggtgttgt atcaacaatg gaacgatttg tagcattttt aactgaagaa
    19561 actaaaggtg cattcccaac ttggttagcg ccaaaacaag ttcaaatcat tccagttaac
    19621 gttgatttac attatgatta tgcgcgccaa ttacaagatg aattgaaatc tcaaggcgtt
    19681 cgtgtaagta ttgatgaccg taatgaaaaa atgggttata aaatcagaga agctcaaatg
    19741 caaaaaatac cttatcaaat cgtagttggg gataaggaag ttgaaaataa tcaagtgaat
    19801 gtgcgtcaat atggatcgca agaccaagaa acagttgaaa aagatgaatt tatctggaat
    19861 ctagttgatg aaattcgttt gaaaaaacat agatagacag ttgtcgcaat aaaatgcttt
    19921 aaaactttta ttgcgtatca agttttacag ggttgattat gcgtgatgaa tcctgtatat
    19981 tacaagttag ttaaaatatt aaattgagtt agaggttgca tgtttaatta gtaacttgtc
    20041 agaagtattt atggtacata agttgaacaa gtgaaaggta aagatgccga aatagatata
    20101 aaccataaat tatatctatt gggacagttt tcgaatagga actgtactgt cacagaatgt
    20161 gatgtgctac cttatataga taattgccaa agtggttgca tatcttaaag gtatgtagcc
    20221 acttttttac ttttaatatc actatgttct gtaaaaaagg gtatgaaagt gaataaaggt
    20281 tatttatttc ttggcctcta aaacatggaa agggagctta tatgtcaaaa gttcaaaatg
    20341 aaagtaacaa tgttgtcaaa aggggactta aagatcgtca tatttctatg attgcgattg
    20401 ggggttgtat tggtacaggt ttatttgtaa cttctggtgg agcaattcat gatgcaggtg
    20461 ctttgggtgc attaatagga tacgcaatta tcggaataat ggtatttttc ttaatgacgt
    20521 cacttggcga aatggctacg tatttgccag tatcaggttc atttagtaca tatgctacaa
    20581 gatttgttga tccatcttta gggtttgcgc ttggttggaa ctattggttt aactgggtag
    20641 tgactgtagc agcagatatt acgattgcag cacaagtcat tcaatattgg acaccattgc
    20701 aaggcatacc cgcttgggca tggagtgcgt tgttcttagt tataattttt agtctgaatt
    20761 cgttatcagt tcgcgtctat ggtgaaagtg aatactggtt ggcattgata aaagtggtta
    20821 cagttattgt tttcattgca attggtttat taacgattgt cggaatcatg ggtggtcatg
    20881 ttgtaggatt cgaaatattt aataaaggtg aaggtccaat tcttggtggc aacttaggag
    20941 gaagtttgtt atcaattcta ggtgtattct taatcgctgg tttctcattc caaggtactg
    21001 agttaattgg tattacggct ggtgaatcag aaaatcctga acgtgctgtg ccgaaagcaa
    21061 ttaaacaagt attctggaga attttattat tttacatttt agccattttt gttatcggta
    21121 tgttaattcc ttatgatagt agtgcattaa tggggggtag tgataatgta gcaacgtctc
    21181 cattcacatt agtgtttaaa aatgctggat ttgcgtttgc agcatcattt atgaatgcag
    21241 tcattttaac gtctgtgtta tcagcaggta actcaggtat gtatgcttca actcgaatgc
    21301 tatattcaat gagtaaagat aaattagcgt ttgaagcatt tggtaaaaca aataaaaacg
    21361 gtgtgccata tatgtcgtta ctcgttacag caatcattgt agtgataatt ttcgtattac
    21421 aaagccttac aaaaggtgca tacgaatata tcgttgcagc aagtggttta actggtttta
    21481 ttgcttgggt aggtatagca gtaagtcatt atcgatttag aagagcattt gataaacaaa
    21541 attatgacaa atctaaatta aaatatactg ctaaattatt cccattcgga ccattatttg
    21601 ctggcttttt atgtattgta gtaattattg gtcaagatgt tgactttatt aaaacaggag
    21661 attttaattt tagtcgcttc ttaattacgt atatgggaat tccagtattt ttagcattct
    21721 ttatttatca taagcttcga tataaaacga aaaaaattcc attagaacaa gtagatttac
    21781 gtcaagacgt gtcaatggat gaaataaaag gttaaaataa aaataattac agttatagtg
    21841 ttgacttaaa tgtgaattgt tgatatgatt aataacgttg aatacgaaac gaaccaagta
    21901 gaagcacccg cttctcacct gtagcgacgc ataaagcagt tggcaggtta atcacattac
    21961 acataactgt gtataaggaa gagcgggtat tttatacctg ctcttccttt ttgcttggtg
    22021 caaatttcgt aaaaattttg gaggtgtcaa ccatagcaaa agatcaaact caaatcaatg
    22081 acaaaattcg tgcaaaagaa ttacgtttaa tcggtcaaga tggtgaacaa attggtgtta
    22141 aatcaaagcg tgaagcttta gaaatggctg aacgtgtaga tttagactta gtggtcgttg
    22201 caccgaatgc gaaaccacca gttgcaagaa ttatggatta cggtaaattc aaattcgaac
    22261 aacagaaaaa agaaaaagaa atgaaaaaga aacaaaaaat tatcaatgtt aaagaaattc
    22321 gtttaagtcc aacaattgag gaacatgatt tccaaacgaa gttgaaaaac ggacgtaaat
    22381 tcttaactaa aggcgataaa tgtaaagtat ctattcgttt cagagggcgt gccattacgc
    22441 ataaggaaat tggtcaacgt gtgctagaaa aatatgcaga tgaatgcaaa gatatagcaa
    22501 cagttgaaca aaaacctaaa atggacgggc gtcaaatgtt tatcatgtta gcgccaacag
    22561 ctgaaaaata attaaagcta ttacaggagg acttatatta tgccaaaaat gaaaactcac
    22621 cgcggagcag ctaaacgtgt taaaagaact gcttcaggtc aattaaaacg ttcaagagct
    22681 ttcacatctc acttattcgc aaacaagagc actaaacaaa aacgtcaatt acgtaaagct
    22741 agattagtgt ctaagagcga tatgaaacgt gtaaaacaat tattagcata caaaaaataa
    22801 gaacaaatac agaaatcggt aggaattacc taaggaggaa ttttttatgc cacgagttaa
    22861 aggtggaaca gtaacaagag cgcgtcgtaa aaaaacgatt aaattagcta aaggttactt
    22921 cggttcaaaa catacattat acaaagtagc taagcaacaa gtaatgaaat caggtcaata
    22981 tgctttccgt gaccgtcgtc aacgtaaacg tgacttccgt aaattatgga ttacacgtat
    23041 caacgcagca gctcgtcaac atgaaatgag ctactcacgt ttaatgaacg gtttgaaaaa
    23101 agctggtatc gacattaacc gtaaaatgtt atcagaaatc gcaatttctg acgaaaaagc
    23161 atttgctcaa ttagtaacta aagctaaaga tgctttaaaa taataacact tataaaaaag
    23221 agatggcatt gtcatctctt ttttattttt taaatagcaa aatgccacat ttaatcgcta
    23281 aaatatgaca tatttgctaa aatgcattta agagaacaaa cattaaagtg aggagtttta
    23341 tacgatgtct aaatttgatg aacaaatcat tgtagtacct agagaaatta tttttaataa
    23401 tgaaaagaat actttcaatg ggtttttaaa taagaataaa ccagaaggtc aaaatatttt
    23461 tgatgcgcta agtcaatatg aagttaagcg acgcggcgat atggaagagg atccatcata
    23521 taaacaactc atttcttatt gtttacttga aaatgagcat ggcgagatat tagtgtatga
    23581 acgattatct ggcggtggag aagctcgatt gcatggacaa tcttcaatag gtgtaggcgg
    23641 tcatatgaat gatgttccag gagcagaatc tattaacgaa gtattgagag ttaatgcaca
    23701 gagagaatta gaagaagaag taggtttaag tgagcaagat tcacaaaata tggaatatat
    23761 cggttttatt aatgacgata ataatgaagt gggcaaggta catattggtg ttgtatttaa
    23821 aatcactgta agtacgaatg atgtagaagc taaagaaaca gatactttac gaataaaatg
    23881 ggttgaaaaa ggcaacatag agtcatatga tgatttcgaa acgtggagtg cattaatcct
    23941 tcaagattta taatcaaacg aggtgacata tatgtcagat attattcagt ttcctaactc
    24001 ttcgaaaaag ttatataaag atataaaacg tgctgaacaa gaccaaaact atgacctgat
    24061 gtatgaatat attgtgcaat atgaacgtca atttgaactc actgaagaaa tagcaatgat
    24121 gaaatgccgt atgttatatg agacggggtc atttcttgaa ttaagagaag aaacaattgt
    24181 cttattgaaa actggcatac aacaatatga tgcattgatg atttattacg tgaaaagttt
    24241 gattggtttg ggacaatatt ttgaagcggt agaagtaatt catcaaatta ttgatgaagt
    24301 caaagatcac aaaacaagaa tggcattaca tcctcttaaa gaatttgcta agtcaaaatt
    24361 aattgaagat gaaaaacgat tgactcagtc attagctgat tttgatacgt tatcaatgag
    24421 ggaacagacg cacttgattt tgaagttaat agacaatggt cattttcaat ttcaagaaac
    24481 ggtattatat atattaaaat ctaatacgta cagttataac ctcattagtt taatgattga
    24541 gtatttaagg ttcgcaaatt gtacacaaga actgacaatt gaaaagtatg gtatggatgt
    24601 aacttttgta ccagctaatt taaaagggct agaacataca acacttaaag aaaaagttat
    24661 acctaacgtt atggaaacat taaatgatgg tgctttacat atcgctgaag aagcacacca
    24721 tattatgaat aatcactcta tcatgattta tccaattgat attgaaactt tgtttgaaac
    24781 gaataaatgg attaatgcat acgaatgtta tttcaaaaat atgttaggac ttaaatgtga
    24841 attacaaagt atcgatgcat ttaattttat tcagcaatta gatttgaata ataatagtta
    24901 aattgttgca aaataatact cagcatttta atatagtttt gacgaaaaaa gttgatttac
    24961 aaatatataa acgttgtgat ttcaatgttt gtatagggaa tagacctatg atataatagg
    25021 gaagttgaaa aaataaatta gttaatcatg gaggtatata tacatgacag caacttggga
    25081 aaaaaaggaa ggtaacgaag gtttattaac tgttactgtt cctgcagaaa aagtaaacaa
    25141 agcgttagat caagcattca aaaaagtggt taaacaaatt aacgtacctg gattccgtaa
    25201 aggtaaagtg ccacgtccaa tttttgaaca acgctttggt gtagaagcat tatatcaaga
    25261 tgctatcgac attttattac cagatgctta tggtgaagca attgacgaaa ctgatattaa
    25321 accagttgca caaccagaag taagtgttac tcaaattgaa aaaggtaaag atttcatttt
    25381 tgaagcaaca gttacagttg agccagaagt taaattagga gactataaag gtcttgaaat
    25441 tgaaaaacaa gaaactgaat tatctgatga tgagttacaa gaagcgattg accacagctt
    25501 aggacattta gctgaaatgg tcgttaaaga agatggtgtt gttgaaaatg gcgacacagt
    25561 taacatcgac tttagtggtt cagttgacgg agaagaattc gaaggtggac aagctgaagg
    25621 ttacgattta gaaatcggtt caggttcatt catacctggt ttcgaagagc aattagaagg
    25681 tatgaaagtt gacgaagaaa aagatgttgt cgtaacattc ccagaagaat accatgctga
    25741 agaattagcc ggtaaagaag caactttcaa aacaaaagtt aacgaaatta aatttaaaga
    25801 agtcccagaa ttaacagatg aaattgctaa tgaattagat gcagaagcaa atacagtaga
    25861 cgagtacaaa gaaaacttac gtaaacgttt agctgaacaa aaagctacag atgctgaaaa
    25921 tgttgaaaaa gaagaagcga ttacaaaagc tactgataat acaacaatcg atattcctga
    25981 agcaatggtt aatactgaat tagatcgtat ggtgtctgaa tttgcacaaa gaattcaaca
    26041 acaaggttta gatttacaaa cgtacttcca aatctcaggt caagatgaaa ctcaattaag
    26101 agagcaaatg aaagacgatg cagaacaacg tgttaaaact aacttaactt taactgcgat
    26161 cgctgaagct gaaaaaatcg aagctactga tgaagatatc gataaagaat tagaaaaaat
    26221 gagtaaacaa tttaatatct cagttgaaga tatcaaaaat actttaggta atactgatat
    26281 cattaaaaat gatgttcgta tccaaaaagt tatcgattta ttaagagata acgcaaagtt
    26341 cgttgaagga actaaagaag attaatcttc attaaatatt aaattacaaa aatgagtagc
    26401 agatgcatag cttatgtatc tgctactatt ctttaagcaa aaagtttgta tgttaatatg
    26461 ttgcattgta acatccaatc tagtatagtc tttaacgaat aggggtgtaa aaagaatgtt
    26521 taaattcaat gaagatgaag aaaatttgaa atgctctttc tgcggaaaag accaagatca
    26581 agtaaaaaaa cttgtagcag gaagtggtgt atatatttgt aatgagtgta ttgaattatg
    26641 ctcagaaatc gtcgaagaag aattagctca aaacacttct gaagcgatga cagaattacc
    26701 tactcctaaa gaaattatgg atcatttaaa cgaatatgtt attggtcaag aaaaagctaa
    26761 aaaatcttta gctgtagctg tttataacca ctataagcgt attcaacaat taggaccaaa
    26821 agaagatgat gttgaattac aaaaaagtaa cattgcatta attgggccaa caggtagtgg
    26881 taaaacatta ttagctcaaa ccttagccaa gacgttgaat gtaccatttg caattgcaga
    26941 tgcgacaagt ttaactgaag ctggttatgt aggcgatgat gttgaaaata tcttgttgag
    27001 attaattcaa gcagctgact ttgacattga taaagccgaa aaaggtatta tttatgtaga
    27061 tgaaattgat aaaattgcac gtaaatctga aaacacatct ataacacgtg acgtttcagg
    27121 tgaaggtgtt caacaagcat tgcttaaaat cttagaaggt acgactgcaa gtgttccgcc
    27181 acaaggtgga cgcaaacatc caaaccaaga aatgattcaa attgatacaa caaatatctt
    27241 atttattctt ggtggtgcct ttgatggtat tgaagaagtg attaagcgcc gtcttggtga
    27301 aaaagttatt ggtttctcaa gcaatgaagc tgataaatat gacgaacaag cattattagc
    27361 acaaattcgc ccagaagatt tgcaagccta tggtttgatt cctgaattta tcggacgtgt
    27421 gccaattgta gctaatttag aaacattaga tgtaactgcg ttgaaaaaca tcttaacgca
    27481 acctaaaaat gcacttgtga aacaatatac taaaatgctg gaattagatg atgtggattt
    27541 agagttcact gaagaagctt tatcagcaat tagtgaaaaa gcaattgaaa gaaaaacagg
    27601 tgcgcgtggt ttacgttcaa tcatagaaga atcgttaatc gatattatgt ttgatgtgcc
    27661 ttctaacgaa aatgtaacga aggtagttat tacagcacaa acaattaatg aagaaactga
    27721 accagaacta tacgacgcag aaggcaattt aattaataat agtaaaacat cagcttaatc
    27781 attgatgtgt tataaaagtg aaaagctcca atcaaagttg acgctcaaat agtttaactt
    27841 tgatcggagc ttttttataa tgtataccga aaggttcaaa tattatataa tggtagaatt
    27901 gaaagagaat ataaaaaagt gaggatataa aatgaaagtt aatcctaata atatagaatt
    27961 aatcattagt gcagtaaaag aagaacaata tccagaaaca gaattgtctg aagttgcact
    28021 gagcggtcga tctaatgtag gtaagtctac atttatcaat agtatgattg gcagaaaaaa
    28081 tatggcacgt acatcacagc aacccggcaa aacgcaaacg ttaaattttt ataatataga
    28141 tgaacaactt atttttgtgg atgttccagg gtatggatat gctaaagtaa gtaaaacaca
    28201 acgtgaaaaa tttgggaaaa tgattgagga atatataact aagagagaga atttgcaatt
    28261 agttattcaa ttagttgatt taagacatga tccaacacaa gatgatatct taatgtacaa
    28321 ttatttgaaa cattttgata ttcctacttt agttatatgc actaaagaag acaaaattcc
    28381 aaaaggtaag gttcaaaagc atattaaaaa tattaagaca caattagata tggacccaga
    28441 cgatacaatt gtaagttatt catcaattca aaataataaa caacaacaaa tatggaattt
    28501 aattgaaccg tatatttcat agtttttgta cgtcaaaact tatacaaaaa ttttaaaaat
    28561 aatgtaagca cgaaactttt aattagtaca caattgataa catttttcaa cgttcatcat
    28621 tttgtcaaaa actcaaaagt aaattagaaa gattataatt tatttaagca tcgtacttaa
    28681 ttggatttta aattatgtta taatatttgt attgttagta tatatggggg cgtttcaaat
    28741 gcattttatt gcaattagta taaatcatcg cacagctgat gttgcactaa gagagcaagt
    28801 tgcttttaga gatgatgcct tacgaattgc ccatgaagat ttatatgaaa ctaaatctat
    28861 tttagaaaat gtcatattat caacatgtaa tcgaactgaa gtatatgctg ttgttgatca
    28921 aattcacaca ggtcgttact atattcaacg atttctagct cgtgcatttg gatttgaagt
    28981 agatgatatt aaagcaatgt cagaagtaaa agtgggggac gaagcagtag aacatttatt
    29041 gcgtgtcact tctggtttag attcaatcgt acttggagaa actcaaattt taggtcaaat
    29101 aagagatgca tttttcttag cgcaaagcac aggtacgaca ggaacaattt ttaatcatct
    29161 atttaaacag gcaattactt ttgcaaaaag agcacataat gaaacagata tagctgataa
    29221 tgctgtaagt gtgtcttatg ctgcggtcga gttggcgaaa aaagtatttg gcaaattgaa
    29281 aagtaagcaa gctatcatta ttggtgcagg ggaaatgagt gaattatcac tattaaatct
    29341 tcttggttct ggaattactg atattacagt agtaaataga acaattgaaa atgctatgaa
    29401 attagcagca aagcatcaag tgaaatatga tgaactatca tcattaccaa atttacttga
    29461 aagtgcagat attgtgatta gttcaacgag tgcacaatct tatatcatta caaatgaaat
    29521 gatagaaaga attgcagaaa atagaaagca agattcacta gtattgattg atattgcagt
    29581 tcctcgagat attgaacctg gtattagtgc catcacaaac atctttaatt atgatgttga
    29641 tgacttaaaa ggtttagttg atgcaaactt acgtgagcga caattagcgg ctgcaacaat
    29701 ttcggaacaa attcctgcag aaatacatgc acacaatgag tggattagta tgttgggtgt
    29761 tgttccagtg attagagctt tacgtgaaaa agctatggca attcaagcag aaacgatgga
    29821 cagtattgat cgtaaattgc cagggttaag cgaaagagaa cgtaaaatta tatctaaaca
    29881 tacaaaaagt atcatcaatc aaatgttgaa agatcctatt aaacaggcca aagaattaag
    29941 tagtgataag aaaagtaatg aaaaattaga gctatttcaa aatatatttg atatcgaagc
    30001 tgaatgtcct catgagcaag cgaagcaaca aaaagaaagc aaggtcaagg aaatttcagc
    30061 gcgacgtatc tttagttttg aataagcata ttaatatggt gataatatgc aagaaaacct
    30121 gtttattcga ttcaatgaaa ttatattatt aatatactta atcagtatca tttgctattt
    30181 ttatgatttt gtacaaaaaa gtcataagat tagaagttta ggcatatatt tattggggat
    30241 tgtttgggtt ttacaaacaa tctctttatc tatttttatt atacaaacta gacatattcc
    30301 attagggtct atttcagatg tattctatac tttaagttgg ctgattattt caatctcttt
    30361 aattcttaac cttatcaaag ttttgaattt ctctgtgttt ttcttgaatt taatagggtt
    30421 aacactttta gggatgaata catttcaacc gacacattat actaataagg ttcagaaaat
    30481 tgctgttgta gacgagttac tgttagtcca tatcggtcta gctgtgttaa gttatgtctt
    30541 ctttgcctta gcttttgtca atgcattact gtatatcatg caataccgta atttgaagga
    30601 aaaacgtttt gatcaaaaat actttagaat tggtagcgtt gctacacttg aatcaattgt
    30661 gttttactca acgttaagtg ggtggataat tctcatattt agtattattt taggtacaca
    30721 gtggggtgta atttcagttg gtgaacgtat attcattgac ccgaaggtaa tactctcttc
    30781 aattattaca gtattatatg gaagttatat tttacttaga atcaacaaat ggttgaattc
    30841 aagatattta atatactata acattatttt attttgctta aatatgatta atttattttt
    30901 tgcaacacat tttgtgaatt gagaaaataa tctagagcaa tccgttccta ggaggaattg
    30961 aacatgcgta aattagtcgt tggctccaga agaagtaaat tagctttaac acaaagccag
    31021 caatttattg ataaattaaa agctgtcgag ccaaatctag aaattgaaat taaagaaatt
    31081 gtcacgaaag gcgatcgtat agtagataaa caattgtcta aagtcggagg caaaggctta
    31141 tttgttaaag aaatacaaca tgaacttttt gaaaaaaata tcgatatggc aatacactcg
    31201 cttaaagacg taccaagtgt aattccggaa ggtttaacat taggttgtat ccctgataga
    31261 gaattacctt ttgatgcgta tatttctaaa acacatacac cactatccca attgccagaa
    31321 ggcagtatta ttggtactag ttcattacgt cgtggtgcac aaatattatc taagtatcct
    31381 aatttagaga ttaaatggat tagaggtaat atagatacac gattagaaaa gttacaaact
    31441 gaagattatg atgcgattat tttagctgca gctggtttaa gaagaatggg ctggtcagat
    31501 gatattgtaa catcttatct tgatagagat acattgttac ctgcaatcgg acaaggtgct
    31561 ttagggatag aatgtcgtag tgacgatgaa gaactattaa cattattaag caaagtacat
    31621 aatgatgagg ttgcaaaatg tgtgactgct gaacgaacgt ttttagcaga aatggatggt
    31681 agttgtcagg tgccaattgc aggatatgct acaatctcag atcaaaacga aatcgaattt
    31741 acaggtttaa ttatgacccc agatggtaaa gaacgatttg aatatataat gaacggaaca
    31801 gatccggttg agttaggcaa aacagtgagc aacaaattaa aagagcaagg tgcttatgaa
    31861 attataaaac gcttaaatga acaacattaa ataatggagt gacgtttaaa tatgaagcca
    31921 gttgtagtta tgacacaaac aaatgacatg caaagtgatt tagtatcaat tattcacaaa
    31981 ccctttattg atataaagcc actaaatttt gatattcatt tgttaaatca acgctatgac
    32041 tggcttattt tttcatctaa aaatgctgtg aaattctttt ataaatatct aaaaggaatt
    32101 aatgttgata acattgctgt gataggaagt aagacagcgc aatattgtga atcacttggc
    32161 attcgagttg attttatgcc aaacgacttt tctcaagaag gatttttaaa atcatttaat
    32221 caaactaacc aaaaaatact tttgccttcg agtgaattgg cgagaccatt gttattagca
    32281 gcgttatcta aagataatga agttgttaaa atagatttat atacttcagt gcctaacaaa
    32341 caaaatatac aagatgttaa agaaatgata gaacatcaac aaatcgatgc attaacattt
    32401 tcaagttcgt cggcagtacg ttattatttt aatgaaggat ttgtaccaaa attcaagtcg
    32461 tattttgcta ttggagaaca aacagcacgg accattaaat catatcaaca accagtaaca
    32521 attgcagaaa ttcaaacact cgaatcacta attgaaaaga ttttagaaag taggggctaa
    32581 aaatgaaatt tgatagacat agaagattga gatcatcagc gacaatgaga gatatggtta
    32641 gagagaatca tgtaagaaaa gaagatttaa tatatccaat ttttgtagtt gaaaaagacg
    32701 atgtgaaaaa agaaattaag tcattgccag gtgtatacca aatcagtttg aatttacttg
    32761 aaagtgaatt aaaagaagct tatgacttag gcatacgtgc cattatgttt ttcggtgttc
    32821 caaactcaaa agatgatata ggtactggtg catacattca cgatggtgtt attcaacagg
    32881 caacacgtat tgctaaaaaa atgtatgatg acttattaat tgttgcagac acttgtttat
    32941 gtgaatatac tgatcatggt cattgtggcg tgattgatga ccatacacat gacgttgaca
    33001 atgataaatc attgccacta cttgttaaaa cagcaatttc tcaagtggaa gctggtgctg
    33061 atattattgc gccaagtaat atgatggatg gttttgttgc tgaaattcgt cgtggattag
    33121 atgaagccgg ctattacaat attcctataa tgagttatgg tgtcaagtat gcatcaagtt
    33181 tctttggacc ttttagagat gcagcagatt cagcgccatc atttggggat agaaaaacgt
    33241 atcagatgga ccctgctaac cgtttggaag cacttcgtga attagaaagt gatcttaaag
    33301 aagggtgcga catgatgatt gttaaacctg ctctaagtta tttagatata gttcgagatg
    33361 ttaaaaatca tacgaatgtt ccagttgttg catataatgt gagtggagaa tatagtatga
    33421 ctaaagcagc ggcacaaaat ggttggatag atgaagaacg tgtcgttatg gaacaaatgg
    33481 tttcaatgaa acgtgcaggt gctgatatga ttattacgta ttttgcaaag gacatttgtc
    33541 gctatttaga taaataaggt tttatattta tgattttcca taaactgtag gaggaattta
    33601 ctttatgaga tatacgaaat cagaagaagc aatgaaggtt gctgaaactt taatgcctgg
    33661 tggtgtaaat agtccagtac gcgcatttaa atcagtagat acaccagcaa tttttatgga
    33721 tcacggtaaa ggttcaaaaa tttatgatat cgatggtaac gagtatatcg actatgtact
    33781 aagttgggga ccacttattt taggacatag agaccctcaa gttattagtc atttacatga
    33841 agcaattgat aaaggtacaa gttttggtgc atcaacatta cttgaaaata aattggcgca
    33901 gctcgttatt gaccgagtac cttcaataga aaaagtgcgt atggtgtcat ctggtacaga
    33961 agctacattg gatactttaa gattagcacg tggttatact ggcagaaata aaattgtgaa
    34021 atttgaaggt tgctatcatg gtcatagtga ttcgttatta atcaaagctg gttctggggt
    34081 ggcaacatta ggattgccgg attctcctgg tgtgcctgaa ggtattgcta aaaatacaat
    34141 tacagttcca tacaatgatt tagatgcact taaaatcgct ttcgaaaaat ttggaaacga
    34201 tattgctggt gtaatcgtag aacctgttgc tggtaatatg ggtgtcgtac cgccgattga
    34261 aggtttttta cagggattaa gagatattac gactgaatac ggcgcattgc taattttcga
    34321 tgaagtaatg actggtttca gagtcggtta tcattgtgca caaggttact ttggtgtgac
    34381 accagattta acttgcttag gaaaagttat cggtggagga ctacctgtag gtgcatttgg
    34441 tggtaaaaaa gaaatcatgg atcatatagc accattagga aatatttatc aagcgggtac
    34501 gttatcagga aatcctcttg caatgacaag tggttatgaa acgttaagcc aattaacgcc
    34561 agagacatat gagtatttta atatgttagg cgatatactt gaagacggtt taaaacgtgt
    34621 atttgctaaa cacaatgtac caataactgt aaatagagca ggttcaatga ttggttattt
    34681 cttaaatgaa ggacctgtaa ctaattttga acaagcgaat aaaagtgatt tgaaattatt
    34741 tgcagaaatg tatcgagaaa tggcaaaaga aggtgtgttt ttaccaccat ctcaatttga
    34801 aggtacattc ttatctacgg cacacacgaa agaagatatt gaaaaaacga ttcaagcatt
    34861 tgatacggct ttaagtcgta ttgtaaaata aatatacgga caaattgaga gcctgaactt
    34921 tgttcaggct ctttttaaat gtatataagg catgggcggc gacttgatag tgaaagtcca
    34981 tatatgcttt gtagtcaaaa ctgctagcgg atattgttat cttaacaaac gtgaagctca
    35041 agcagcaatt tagtcatttt attttttatt gaaagaagtg gaaaacatgg caatgatata
    35101 tagaaataat ttcattgtgt tcgttttatc attttttatt agtattctac tgtattcatc
    35161 gcacgtatta cttccattta tgtttggtcc tattatcgca tcaatcattt gtgtaaaagt
    35221 tttcaaactt gatattaaat ggccattctt acttagtgaa ttaggtattg tactattagg
    35281 tgtgcaaatc ggatcaacgt ttacgaaaaa tgtcgttatg gatattaaaa acaattggct
    35341 ttcgattatt gttgtatcta tttcgatatt attaattgca atagtaatgg catttgtttt
    35401 taaaaaaatt gcacgtatta atacagaaac agctatttta agtgttatac caggagcact
    35461 aacacaaatg ctggtcatgg ctgaacaaga caaacgtgct aatttgttag ttgttagctt
    35521 aacgcaaaca tcacgaatta tatttgttgt tgttttagta ccgttcattt catatttttt
    35581 tcatgatggt aacatgcatg cgaatggaaa gttaacaaaa gtcttgcctt tatcacaagt
    35641 attaaacata gggcaaatag ttattttagc gatagctatc tttatagttt atctaattat
    35701 gtctaaaata aagtttccaa catttcaatt attagcacca ctcattgtat taattgtttg
    35761 gaatttttct acaggtttaa catttacact agatcattgg ttgttgaaca tggcacaact
    35821 aatatatatg attagaattg gagttcaaat tgcgcattta ttgtcagatt taaaaggtag
    35881 actagcaatc gcaattacaa ttcaaaatat tatgttgatt attggtgcgc taatcatggt
    35941 ttatatcata catttctttg ataataatcc tattaatgaa ttgtttcttg gtgcagcacc
    36001 aggaggggtt aatcagatag ttttagttgc attagctact ggtgccgatg ttgctatgat
    36061 tacgagttat catattttcc gaatcttttt catcctattt attattgcac caggaattaa
    36121 ttacttttta aaatatcgtt cgaataagcg tgaagattag aaactatcat ccatctaagt
    36181 tgaattatgc ttaggataga attcattatt aaggtttaag caaaggttgt acatctattt
    36241 ttagattaag atatctaata ttagcagagc attaacttgg aactttaact tatgatttga
    36301 ataaattttc acattaaaaa acgagacagt ctattagtta aaaatagatg atgtctcgtt
    36361 tactttatga tgctatagca aattaattat gtttaggctt tgatggacaa tcttgtaaat
    36421 gtgcatcata taaaccggct gcttctaaaa acgaaaatac tgttactgga cctaaaaatt
    36481 taaacccgta ttgttttaaa tctttagata gttgtgttgc agtatcatca actgtgatac
    36541 gatcagaagc atgttcatac tgcaaatctt taggcttacc atttacatat gaccataaaa
    36601 atttactaaa actaccatat gcttgttcaa tttttaaata cccttgagct tgattaacaa
    36661 ttgcttctaa ttttttacga tgatgaacga tatttggaaa agtcattaag cggtcgatat
    36721 cttgagcggt catttgtgct accttttctg gttcgaaatc ataaaatgct tcttcatagg
    36781 cttctttctt ttttaaaata gttaaccaag atagcccagc atgttgtgat tctaatgcta
    36841 aaagtttaaa caatgccttg ctatcataga gcggttgtcc ccatacatga tcatgatagt
    36901 ctaagtagac tggatcttta gtaccaaatg cgcattcatt cattttattc ccacctatac
    36961 attgacttat tttgtcaatc attatactat aagttaagag tttaatatag gatttgggaa
    37021 gagtaagttc aaattatttt aaagagagca tatgatttgg tgtgagtatg taaatgattt
    37081 ttactgaagg tagcccacag agtatcttag ctgaacgtga ttaatagtag gctaagacgt
    37141 gtttgagcgt taatcataat ctagagtgct tgaagagatg aagcatttca aatttcacga
    37201 tgatagtatt ggtatgatta ttccaattat gttaaaagag tgaatttgat attaatcatc
    37261 cttcttgaat ataggtggta ccacggaata tccgtcctat ttgtatatag gatggatatt
    37321 tttatttttt taggaggtat agcaaatgga aatgaaacca aaatatgatc ctcgtgaagt
    37381 tgaagcggga cgttatgaag aatgggtaaa gaatggttat tttaaaccgt cagaagataa
    37441 atcaaaagaa acatatacaa ttgttatccc gccaccaaat gtaactggta aattacattt
    37501 aggacatgca tgggatacga ctttacaaga tatcattaca cgtatgaaac gtatgcaagg
    37561 atacgatacg ttatacttac caggtatgga tcatgctggt attgcgacac aggcaaaggt
    37621 agaagctaaa ttaaatgaac aaggaataac tagatatgat cttggtcgtg aaaagttttt
    37681 agaacaggca tgggattgga aagaagagta tgcgtcattt attcgtgcgc aatgggctaa
    37741 attaggtcta ggtttagatt atagtagaga acgttttact ttagatgaag gtttaagtaa
    37801 agcagttaaa aaagtttttg ttgatttata caataaagga attatttatc gtggcgaacg
    37861 tattataaat tgggatccta aagcacgtac agctttatct gatattgaag taatacatga
    37921 agatgttcaa ggtgcgtttt atcattttaa atatccttac gctgatggtg aaggttttat
    37981 tgaaattgca acaacaagac cagaaacgat gttaggtgat acagcgattg ttgttaaccc
    38041 taatgacgaa cgatacaaag atgtaatcgg taaaactgtt atattaccaa tcgtaggacg
    38101 cgaactgcct attttagcag atgagtatgt tgatatagac ttcggttctg gtgctatgaa
    38161 agtgacacca gcacatgacc ctaatgattt tgaaattggt caaagacatc aattagaaaa
    38221 tattatcgtt atggatgaaa atggtaaaat gaacgacaaa gcgggtaaat atgaaggtat
    38281 ggaccgtttt gattgtcgta aacagctagt taaagattta aaagaacaag atttagttat
    38341 caagattgaa gatcatgttc attctgtagg tcattcagaa cgatctggcg ctgttgttga
    38401 accatattta tcaacacaat ggtttgtgcg catggaagac ttagcgaaac gttcattaga
    38461 taaccaaaaa acagatgatc gtattgattt ttatccgcaa cgtttcgaac atacatttaa
    38521 ccaatggatg gaaaatatta gagattggac gatttcaaga caattatggt ggggtcatca
    38581 aattccggct tggtatcata aagaaacagg cgaaatatat gttggagaag aagcgccaac
    38641 tgatattgaa aattggcaac aagatgaaga tgtattagat acgtggttct caagtgcttt
    38701 atggcctttc tctacgttag gttggcctga tttagaaagt gaagacttta aacgatacta
    38761 cccaacaaat gccttagtta caggttacga tattatcttt ttctgggtag cacgcatgat
    38821 attccaaggc ttagaattta cagatcgtcg tccatttaat gatgtattat tacacggttt
    38881 agttcgtgct gaagacgggc gtaagatgag taaatcatta ggtaatggtg tggatccaat
    38941 ggatgttatt gacgaatacg gtgctgatag cttgcgttac ttcttagcaa caggttcatc
    39001 tccaggacat gatttaagat actcaactga aaaagttgag tcagtgtgga actttatcaa
    39061 taaaatctgg aatggggcac gtttcagttt aatgaatatc ggtgaagact ttaaagttga
    39121 agatatcgat ttaagtggta acttatcatt agcagataaa tggattctaa cacgtttaaa
    39181 tgaaacgatt gcaacagtta ctgatttaag tgacaaatat gaattcggcg aagttggacg
    39241 tgcattatat aatttcattt gggatgattt ctgtgattgg tacattgaaa tgagtaaaat
    39301 tccaatgaat agtaatgatg aagaacaaaa acaagttaca cgttcagtat tgagttatac
    39361 tttagacaat attatgagaa tgctacatcc attcatgcca tttgtaacag agaaaatatg
    39421 gcaaagttta ccacatgaag gtgacacaat tgttaaagct tcatggccag aagtgcgtga
    39481 atcattgatt tttgaagaaa gtaaacaaac aatgcaacaa cttgttgaaa tcattaaatc
    39541 tgtaagacaa tcacgtgtag aagtaaatac gccattgtct aaagaaatac ctattttaat
    39601 tcaagctaaa gataaagaaa ttgaaacaac tttatcacaa aacaaagatt atttaatcaa
    39661 attctgtaat cctagtacct taaatattag cactgacgtg gaaattcctg agaaagcaat
    39721 gacatcagtt gtaattgcag gtaaagtggt attaccatta gaagggctaa ttgatatgga
    39781 taaggaaatc agccgtttgg aaaaagaatt agctaaactt caaagcgaat tagatagagt
    39841 agataaaaag ctctctaatg aaaactttgt aagtaaagca cctgaaaagg ttataaatga
    39901 agaaaaacgt aaaaaacaag attatcaaga aaaatatgat ggtgtgaagg caagaattga
    39961 acaattaaaa gcataggagt tagtaacaat gaattaccta gagagcttgt attggataca
    40021 cgaaagaact aaatttggca tcaaaccagg tgttaaacgt atggaatgga tgctagcaca
    40081 atttaataat cctcaaaata acattaaggg tattcatgta ggtggcacaa atggtaaagg
    40141 ctctacagtt gcttacctta gaacagcttt agttgaaaat ggttatgaag taggtacatt
    40201 tacgtcgccg tttattgaaa catttaatga acgaattagt ctaaatggtg tgccaatatc
    40261 aaatgacgct attgtagaat tagtatcacg tattaaacca gtaagtgaaa tgatggaacg
    40321 tgaaacagat ttaggtgttg caactgaatt cgaaataatc acagcgatga tgtttttata
    40381 ttttggtgaa atacatcctg ttgattttgt cattgttgag gctggattgg gtataaagaa
    40441 cgattcgaca aatgtcttta caccggtttt atcaatctta actagtatcg gtctagacca
    40501 tacagatatt ttaggtggta cttatctaga tattgctagg gataaaggcg cgattataaa
    40561 gcctaacgtt ccagtgatat atgctgttaa aaatgaagat gcattaaaat atgttcgtga
    40621 acgcgcaatt gaacaacatg caaagccaat tgaattagat agagaaattg ttgttgtatc
    40681 gcaaaatgat gaatttactt accgttataa agattatgaa ttagaaacaa tcattttaag
    40741 catgttaggt gaacatcaga aacaaaatgc tgcattagcc ataacagctc ttattgaatt
    40801 aaatgaacaa ggattaattg aattagattt caataagatg atagacggta ttgaatcagt
    40861 tcgttggact ggacgtattg agcaggtgca tgacaaacct ttaatcattt tggatggcgc
    40921 acataattca gagagtatag atgctctaat tgatacaatt aaacagtacc atgataaaga
    40981 aaaagtagat attttgttct cagcaataaa cggaaaaccg attaacgaga tggtcaaaca
    41041 tttaagttta attgcgcata cgttttatgc aactgaattt gattttccga aagcgttacg
    41101 caaagaagaa attgtaggta gtattgaaaa tgatgaaata caattagtag atgactacgt
    41161 tgaatttata aaaaattatc aaggtgatac attagtaatt accggtagtc tgtatttcat
    41221 aagtgaagtt aaatcaaaat tagactttga agcattataa aactaaaact aaaatagcca
    41281 tgaagaatct atttaatcaa taggtagagc ttcatggtta ttttttatgc tctaaatcgt
    41341 atgtgtttat aaggttcaat ttcttgccat tattaatttt gccgagcagt aatgggttgt
    41401 taaagtatcc atcaattatg cttagcaata ttgaaaaatt taaatgaaaa agcggttcaa
    41461 ttcttgaaaa atagtgtata tttacatttt ggtaataact aactataatt tacataagga
    41521 ggttgtcatt tggtagtatt gttatcttat agttgcagtt gtatttttag ttttctatat
    41581 caatttatat ctatagaaga aacgtcattt gattatttac atagaagatc gaaatgtgat
    41641 tattgtaatt cgtcactcaa atggtatgaa ttaatgccga ttattagttt tttattatta
    41701 aaagggcgat gtcgaaactg tcgaaagcgt atttccctaa cacatttctt aggggaaacc
    41761 tttgctttaa tacctatcgt ctttattaag tatgatttca catacgtaaa tgctacgcta
    41821 tttataacta catatgtttt tctgcttatt tttactatga ccgatatcac ttctttaatg
    41881 ttagattgtc gcttaattat aatttattgt atcgtttctc tctcgttaag tatgatttat
    41941 ccagtagctt ttatcattat tagtatgacc acgcatatat tctacttttt atttcgggca
    42001 tatattggtt atggtgacgt tttactaata tctgcacttt ctttgttttt ccctctccaa
    42061 ttcactattt atgtcatttt atttacattt gtcattgctg gtttagttgc tttaattacc
    42121 atgatattta agccgattaa actattaccc cttgttccat ttatatttat ttcctttttt
    42181 atcaattcac ttttttataa tgatatccat caatttttag gaggcgtata tttttgaaaa
    42241 ttaaagaaat ggtaacttca gaaatgccaa gagaacgttt gttaagccat ggtgcaaaaa
    42301 gtctttcgaa tacagaatta ttagctatat taattaacac cggaagaaaa ggattctcga
    42361 gcatagacat tagtaatgaa ctgcttaaat ctgcttcgaa tctgaatgaa ttgaaaaaat
    42421 cttcaattaa cgatttgata aaagttaaag ggattggatt acaaaaagca attactttaa
    42481 aagcagcatt tgagttaggg gaaaggatgg ggagaagagc tgaaaataat cgtataaaaa
    42541 taacgcaacc aagtgatgtt gctgattata tgattccaac aatgaaagat ttaacacaag
    42601 aacattttgt cattttattg ttaaattcaa aaaatgtagt gattaaagaa acctgtgttt
    42661 ttaaaggtac attaaatagt tcgattgtac atccacgtga aatttttagt attgcggtga
    42721 gagaaaatgc caatgcaatc atcgcagttc ataatcatcc atccggtgat gtaacgccct
    42781 cacaagaaga tatcataaca acaatgaggt tgaaggagtg tggtttgatt ttagggatag
    42841 atttattgga tcatattata atcggtgata atagatttac cagtcttgta gaagcgggtt
    42901 actttgatga aaatgattga agttgataga attaatgacg tatcttgtgt ataataccta
    42961 cgaagtactt tcattggagg aaaaatagtg actctattta ttattatcgg ggttctcgtg
    43021 ccaatggttt ataccatgca gttaaatatt aaaaatgaac ctgtaacaaa gcgcaatctt
    43081 ttaataacat tagctttatc tacgttaggt attttagtaa ccgcgttagc aggtgtaatc
    43141 gttacgaaac aagcttttcc tttattaagt gtagcaattg gctcaatttt tactggaatc
    43201 gtttggggcc ttttactaag tggtagctac gcgctgatac gatttttatc taacgcattt
    43261 gggcgtaagt aatagtaatc tgttcatcaa gtagtatccg tgcttgaaaa caaactaaaa
    43321 ctcctaatgt ggaactagtt aaaaaattct aaactagtac attaggagtt ttgttatgca
    43381 gaataaattt aattgttaaa ttgaaagtgc ggtaaaaatc cactatttat ttgaaaaaaa
    43441 tcgagcaacc aaattaaacc ttgatatcct aagtaaatac ataataaaac aagtccgaca
    43501 taaattaaaa atcgcaaaat agaaagtcca actctaaaaa ggaggatgac taatagtgct
    43561 attaaaataa ttgttagtat actcaatgct acaaacctcc taatacgctt ttaaatcata
    43621 acaaaattat aagctatttt aaatgaacgt gcatcagtcc taagatatat catgattaag
    43681 gctgaagact gaggaaaata tacataataa aatatataat tagatgaaga cattggaggc
    43741 gaactatgag attgatattt agtatgatta aaaacattat agcagtaata gcaattgtga
    43801 taattgttta tatcgccttg aaatacgcac cttttttaaa agaacaagat tggaatcctg
    43861 taaatcacga taatgatcaa atgaatcaag ttacgcaacc cactaatgat gcccaacatg
    43921 tttatgtaag tggtgagaaa tattcattaa aagaaaatga tttaataaag aatgtaccat
    43981 taagccaaat taaaaatgta tttaaaatga tagataaaca agagtttatg gctgtgtctg
    44041 gaatgaatcg catggcttat aatgatcaat atattatagg tcaaagagga gacgaattta
    44101 ttctttataa atttggagat gagtcaatgc gtgtttacaa tactgaattt gaaatgcaac
    44161 aagacttaaa tgaattaggg caaaatttac aactaaaatc tgaaaatgct tatcaatagg
    44221 aaattattgc ataagaaata tattttagtt atcctgtcac tagaataaat ttaaaaggtt
    44281 ggtgtagttc aaatggcaga acaatcaaaa cagaaacaag ctaatgaaca acaaaaggcc
    44341 cagaacttat tcgcacgttg gagacaactt cagaattcga acagtgaatc ctcgaacgat
    44401 acaaacaaat aattttaaaa tttaaatcga ttatttaaac tactagtgac tggaatgttt
    44461 gctagtagtt tttttgtttt taaaatgcca caaatttttt acggagttgt tcattttcaa
    44521 agtaattcgt agttagtgct ttatttcata atgggagtaa tataaaatag aaaaggatat
    44581 acaatatttt gacataatag aggtgttctg ggtgcttaag ttttttaaaa ataacaaatt
    44641 aattgttgtt ttatgtgcaa ttatcgtttt tattgcatta attgggctgt ccatacgttc
    44701 acaatctcaa tcacctcctg aacaatatat aggtgattct gtgtcttttg gacaacgagt
    44761 tgtgagttat ccagttaatt ttgttgctgg tacgattggg gactttttta aaaaaggaga
    44821 ttctaaagaa tctaaaaata agattagcca gttagaatct aagaaccaac aattagaagc
    44881 ggaaaatgaa aaattaaaaa aagagcttga tttaaaagat atttcaaaat ttgatcctat
    44941 ttctactacg gttttggcaa gaaatccgga tcagtggatg aatacaattg taattgataa
    45001 gggatctaaa tcaggtataa cttcaaatat ggctgtgatg acatcacaag gttttgttgg
    45061 aagagttact aaagttaata aattttcttc acaagttgat ttaatctcaa ctaatacacg
    45121 tgcgggtaaa ttatctgtaa atatacaaca cggttctaaa aatatatttg gtttaattga
    45181 tcgttatgat gaaaagaact cagaacttgt aattagtgac attaataata gagataatat
    45241 ctcaaaaggt gataaagtcg ttacaagtgg attagctgat caactaccaa gtaatttata
    45301 tataggagaa gtgactaagg ttcaaaatga tcaatacggc ttagctaaag aagttagggt
    45361 taagactggt gcggacttaa cagatttgag tcatgtttat gttgcaaaaa gagatcctaa
    45421 aacaattcct gatgatgaaa gcagggataa ataatgcgta cactgtatta ttttttgata
    45481 ggtattttac tattttatat agatactgca attgggcttc ttattccaat gcacataggt
    45541 aagtttgaac ttgtatttgt accacacctt acatttatgt atattttaat gatggtagtg
    45601 tatcgtggct ttggcgtatc attattgctc agcatatttt taggcgtaat gactgatgta
    45661 tactttggta gtatttatgg agtgtactta tttggctata tattgttttt agcacttata
    45721 gatcgattct ttaaaatttt ttacaaagat cattcgatgt tattcattat tatattagcc
    45781 agcaccttat tattagaagt ctatgtggca ttaatatacg gtatgttagg attcattcaa
    45841 tttgatatta ttcattttgt agtctttaga ttattgccaa cattaattat gaattttgta
    45901 ctgttgatta tgctttatcc gttgattata aagttcctta aaaaaacaaa caatgacatt
    45961 gacatgaaac gtcgtcaatg gtaagatgca atagttgagt agttataagt agctacatac
    46021 aaccgctcaa atataggttc aagaacattt ttaatgaaga aagcgatttt cccacagagt
    46081 gcaaacgctt gactcaataa cgaaatgtca cctatgtatg gcgtgactta ttatacagga
    46141 ggtgcaaagt atgtttgcta ttattgaaac aggtggaaaa caaatcaaag tagaagaagg
    46201 tcaagaaatc ttcgttgaaa aattagacgt aaacgaagga gatactttta catttgataa
    46261 agtattattt gtaggtggag attcagttaa agttggagcg ccaacagttg aaggtgcaac
    46321 agttactgct actgttaata aacaaggtcg cggtaaaaaa atcactgtat tcacatacaa
    46381 acgtcgtaaa aattcaaaac gtaaaaaagg ccatcgtcaa ccatacacta aattaacaat
    46441 cgataaaatc aacgcgtaat tattatgatt actgttgata ttacagttaa tgatgaaggc
    46501 aaagtaacag acgttattat ggatggccat gctgaccatg gtgaatatgg tcatgatatc
    46561 gtttgtgctg gagcttcagc tgtattgttt ggtagtgtta atgcgattat aggattgaca
    46621 tctgagagac cagatatcaa ttatgacgac aatggtggtc attttcatat aagaagcgtt
    46681 gatacaaaca acgatgaagc gcaactaatt cttcaaacaa tgcttgtgtc tttacaaact
    46741 attgaagaag aatataatga gaatattaga ttaaattata agtgaggtgc attccgatgt
    46801 taaaattaaa cttacaattc ttcgcatcta aaaaaggggt aagttctaca aaaaacggac
    46861 gtgactctga atcaaaacgc ttaggtgcta aacgtgctga cggtcaattc gtaacaggtg
    46921 gttcaatttt atatcgccaa cgtggtacta aaatttaccc tggtgaaaat gtaggtcgtg
    46981 gtggcgatga tacattattc gctaaaatcg acggcgttgt taaattcgaa cgtaaaggtc
    47041 gcgacaaaaa acaagtttct gtatatgcag tagctgaata attttgtcta gttaacacca
    47101 gaagtgaatc ttctggtgtt ttttactttt tataaaatgt ttttcattat tattttcatt
    47161 atgttattaa aaaatgggaa ttttagacgt tatactaaat gtgcaactgt ataagagccc
    47221 ctaatcacta aactataagg gggacaaagg aatacagttg cagcgtttaa agaataaact
    47281 gtaccacaat tggtgctgag aaatataagt attttaaagc aaagatttat aaaagtaact
    47341 gcataagagc ccctaattat ttacaatata taaggggctc taatatgcta taattattgg
    47401 gaaaatgaaa attatatgta aaagaggtga gatatatgtt tgtcgatcaa gtcaaaatat
    47461 ctcttaaagc cggtgatggt ggtaatggta ttaccgcata cagaagagaa aaatatgtac
    47521 catttggtgg accagctggc ggtgacggtg gtaaaggtgc ttcagtcgta tttgaagtgg
    47581 atgaaggttt aagaacgtta ttagatttta gatatcaacg tcattttaaa gcaagcaaag
    47641 gtgaaaatgg ccaaagtagt aatatgcatg gtaaaaatgc ggaagattta gtattaaaag
    47701 ttccacctgg tacaattatt aaaaatgttg aaacagacga agtgttagca gatcttgttg
    47761 aagatggtca aagagctgta gtagcgaagg gcggtcgagg tggccgaggt aattcacgtt
    47821 ttgcaacacc tagaaaccct gcacctgact tcagtgaaaa aggtgaacca ggtgaggaat
    47881 tagatgtatc tttagaattg aaattattag ctgatgtagg attagtaggt ttccctagtg
    47941 tgggtaaatc gactttatta tctatcgttt caaaagctaa gcctaaaatt ggggcatatc
    48001 attttacaac gattaaacca aatctaggtg ttgtttcaac gcctgatcaa cgtagttttg
    48061 ttatggcaga tttaccaggt ttaattgaag gtgcatctga tggcgttgga ttaggacatc
    48121 aatttttaag acatgtagag agaacaaaag ttattgttca catgattgat atgagcggtt
    48181 ctgaaggtag agaacctatt gaagattata aagtcattaa tcaagaatta gctgcgtacg
    48241 agcaacgttt agaagataga cctcaaatcg tagtagctaa caagatggat ttacctgaat
    48301 cacaagataa tttaaacttg tttaaagaag aaattggcga agatgtgcca gttattccag
    48361 tttcaacaat aacgcgtgat aatattgatc aattattata tgcaatagca gataaattag
    48421 aagaatataa agatgttgac ttcacagttg aagaagagga gtcagttggc attaaccgag
    48481 tattatataa acatacaccg tcacaagata aatttacaat ttcaagagat gatgatggtg
    48541 cttatgtggt aagtggtaat gctattgaaa gaatgtttaa aatgactgac tttaacagtg
    48601 atccagcagt acgtcgattt gctcgtcaaa tgcgttcgat gggtattgat gatgcgctta
    48661 gagaacgtgg ttgtaaaaat ggtgatatcg ttagaattct tggcggagaa tttgaattcg
    48721 ttgaatagga gcgaaacatg atggacaata aagattataa aaagttttat ttaattagag
    48781 aagatgtctt gcctgaatcc gtggttaaaa cattgaagat taaagatgcc ttaaaaagtg
    48841 atccgacatt gtccatttat gatgccgtta aacagtttga tctatctaga agtgcttttt
    48901 ataaatatag agaaacgata tttccagtag acgataaaat gcttgaccat cgagaattta
    48961 cattaatttt atatgtaact gatattgttg gtatgttggc acgtgtacta gatgttatat
    49021 caaagttaga actatctgta ttaacgattc atcaaagtat tccaatggaa gaaaaagcaa
    49081 caataacatt atcactgaat gctaaatcta aagaaacttc agtagaagat gttattggcg
    49141 ctttgagaaa tttagattat gtatcaaaag tagaattaat tagtatgagt atgtaaggat
    49201 gtgcctataa tgtacgcgta tgtcaaaggt aagttaacac atttatatcc tacacacgta
    49261 gttgttgaaa ctgctggtgt tggttatgaa attcaaacac caaattctta tcgttttcaa
    49321 aagcatctag atcatgaagt tttaattcat acatctttaa ttgttcgtga agatgcacaa
    49381 ttattgtatg gatttagtag tgaagaagag aaagatatgt tcttgagttt aattaaagtt
    49441 actggtattg gtccgaaatc agctttagct attttagcga caagtacgcc taatgaagta
    49501 aaacgtgcca ttgaaaatga aaatgatacg tatttaacta aattcccagg aattggtaag
    49561 aaaacggcaa gacagattgt cttagattta aaaggtaaag tgaaaattac tgaagaagat
    49621 agcgattcat tattacaagt agacgctact tcgacggtgc aagatcaatt cgtgcaagaa
    49681 gcaatgttag cgttagaagc attaggttat tctaaacgag agcttgcaaa agttgagaaa
    49741 acgttaaata aaaataaata tgactcagtt gatgaagctg ttaaggcagg tcttcaatta
    49801 gttgtatctt aattttaaat agattaatag gggaagtgtt gtcatgaatg agcgtatggt
    49861 tgatcaatca atgcatagtg aagaaactga tttcgaattg tcgcttagac ctacgagatt
    49921 acgacaatat attggtcaaa attcaataaa aagtaattta gaagtattta ttaaagcggc
    49981 taaacttcgt catgaaccat tagatcatgt attgcttttt ggcccccctg gattaggtaa
    50041 gacaacatta tctaatatca ttgccaatga aatggaagtt aatatacgta cagtatcagg
    50101 gccttcatta gaaagacctg gtgatttggc tgcaatttta tcaggacttc aacctggaga
    50161 tgttttgttt attgatgaaa tacacagact gagtagtgtt gttgaagaag tgttataccc
    50221 tgcaatggaa gatttctttt tagatattat cattggtaaa ggcgatgagg ctagaagtat
    50281 ccgtatcgac ttacctccat tcactttggt aggtgcaaca acgcgagctg gcagcttaac
    50341 aggtccacta agggatcgat ttggtgtgca cttaagatta gaatattata acgaatcaga
    50401 tttaaaagaa atcattatta gaacagctga ggttttaggc acaggtattg atgaagaaag
    50461 tgccattgaa cttgctaaac gttctagagg gactccaaga gtagcaaatc gactattgaa
    50521 gcgggtaaga gacttccagc aagtgaatga agatgaacaa atatacattg aaacaacgaa
    50581 gcacgcatta ggtttacttc aagttgatca acacggacta gattacattg atcataaaat
    50641 gatgaactgt attattaagc agtataatgg cggacctgtt ggtttagata cgattgccgt
    50701 aacaattggt gaagaacgta ttacaattga ggacgtttat gagccatttc ttattcagaa
    50761 aggcttttta gaacgtacgc cacgtggcag aaaagcaaca ccattagctt atgaacattt
    50821 tgcaaagtcg aatgaggaga gagaataacg tgaatattga agaatttgac tatgacttac
    50881 cagaatcatt aattgctcaa acgcctttaa aagatcgtga tcatagtcgt ttattagtca
    50941 tggatagaga aactggtgaa atgaaacatt tacatttcaa agatatcatt gagtatttta
    51001 gacctggtga tacattagtg cttaacgata cgcgagtaat gccagctaga ctttttggtt
    51061 taaaagaaga aactggtgca aaagttgaaa tgttaatgtt aactcaaatt gaaggtaatg
    51121 attgggaagt cttactgaaa ccagctaagc gtattaaagt tggtaataaa ttgaattttg
    51181 gtaatggcaa aattatagct gaatgcataa aagaaatgga tcaaggtgga cgcatcatgc
    51241 gtttacatta tgaaggtatt ttacaagaaa gattagatga attaggggaa atgccactgc
    51301 caccatacat caaagaacgt ttagatgatc cagatcgtta tcaaacagtt tacgctaaag
    51361 aaagtggttc agcggcagca ccaacagcag gattacattt tactgatgag ttattaattg
    51421 aaattaaaaa taaaggtgtt aatatcgcat ttgttacatt acatgttggg ttaggtacgt
    51481 ttagaccggt gagcgtagac gatgtgaatg accacgaaat gcatagtgaa tattatcaaa
    51541 tgacacaaga aacagctgat ttattaaatg atactaagtc caaaggacat cgcattatat
    51601 cagttggtac aacttcaaca cgtacacttg aaacaattcg acgcgatcat gataaatttg
    51661 ttgaaacgag tggctggact aatatattta tttatccagg atttgatttt aaagcaattg
    51721 atggccagat tactaatttt catttaccaa aatcaacatt agttatgcta gtatcagcgt
    51781 ttagtagtcg tgaaaatgtt ctgaatgctt ataaaacggc agtaaattta gaatatagat
    51841 tctttagttt tggcgatgca atgttaatta tataaaaaga atgtgaggat tttgaatatg
    51901 cctgcagtaa catacgaaca cattaaaact tgtaaacaat caggtgcgcg tttaggtatc
    51961 gtgcacacac cacacggttc atttgaaaca cctatgttta tgccagttgg tactaaagca
    52021 accgttaaaa caatgagtcc agaagagtta agacaaattg aagcaaaaat cattttgggc
    52081 aacacatatc atttgtggtt acaacccgga aatgatatta tcaaacacgc tgggggatta
    52141 cataaattca tgaattggga tggtccgatt cttacagatt caggcggttt ccaagtgttt
    52201 agtttaagta atttacgtaa aattacagaa gaaggcgtgg aatttagaca tcatactaat
    52261 gggtctaaat tatttttgag tcctgagaaa tcaatgcaaa ttcaaaatga tttaggatct
    52321 gatattatga tggcatttga tgaatgtcca ccgatgcctg ctgaatatga ttatgtaaaa
    52381 aaatctattg aacgtacaac acgttgggcg aaaagatgtc tagatgcaca ccaaagacct
    52441 gaagatcaag cattgttcgg cattatacaa ggtggcgaat atgaagattt aagagaacaa
    52501 agtgcaaagg atttagtaga attagatttt cctggttatg caatcggcgg tttgtcagtt
    52561 ggtgaaccta aaccggttat gtataaaatg gttgaacata cagagcagtt tatgcctaaa
    52621 gataaaccaa gatatttaat gggtgtagga tctccagatg cgttaatcga atgtagtatt
    52681 cgcggcatgg atatgtttga ttgtgtctta ccgacacgta ttgccagaaa tggtacttgt
    52741 atgacatcgc aaggtcgttt agttattaaa aatgcaaaat ttgcagatga tttaagaccg
    52801 ttagatgaga attgtgactg ttatacatgt caaaactatt caagagcgta tatacgtcat
    52861 ttaatcaagg cagaggaaac ttttggtatt cgtcttacta ctattcataa tttacatttt
    52921 ctgctaaaat taatggaaga tataagacaa gccattcgag aagatcgtct tttagatttc
    52981 aaagaagaat tcttcgagca atatggatta aatgttgaga acccaaaaaa cttttaagca
    53041 agaggagcgt ataaaatgca attttcatta ctaatatata tagtcgtaat ttttgcggtt
    53101 atgtatttct tgatgatcag accacaacaa aaacgtgcga aacagcatcg tgagttgatt
    53161 aataacattc aatctggtca aagaattaca actattggtg gtattaaagg tactgttaaa
    53221 gcagtagatg aaacaactgt tgttattaca gttaatggtc atggtactga attaactttc
    53281 gaaaaacctg ctattaaaca agttgaccct tcataatgag aagtggtatt aaaaaggatg
    53341 aaacgaatag cgtttcatcc tttttaagtt ataagtagta tgtagaatag cttattttta
    53401 ttccaaaaaa agaaattgtg atacataata attgttaaat ttaaattaaa tatatctatg
    53461 actattttta ctccctgctc tagtatgtta atatgtataa ggctaataaa ttttgtaaga
    53521 aagatttaac ttgcgttata aaatagtatt caagtattct aaatacatac gtaaataatg
    53581 aggtgttcat gtgaagaaaa gtagtagaat aattgcgttc ttgcttttag tcgtattgtt
    53641 gtttgccgga atggctgcaa cttataaaag tgttataaag aatgtcaatt taggacttga
    53701 tttgcaaggt ggatttgagg tgctttatca agtcgatcct ttaaataaag gcgacaaaat
    53761 agataaaaaa gccttacaat caacagcgca aacattagaa aatcgtgtta acgttttagg
    53821 tgtttcggaa cctaaaattc aagttgaaga acctaataga attagagtac aacttgctgg
    53881 tgtaacagac cagaatgaag cacgtaaaat tttatcttct caagctaatt taacaattcg
    53941 cgatgcagaa gataaagtaa aattaagcgg ttctgatatt aaacagggat ctgccaaaca
    54001 agaatttaaa caggaaacaa accagcctac agttactttt aaagtaaaag acaaaaacaa
    54061 atttaaaaaa gtaacggaag aaatctctaa gaaacgtgat aatgtcatgg ttgtttggtt
    54121 agatttcaaa aaaggtgaca gctataaaaa agaagcacaa aagaaaaacc ctaaatttat
    54181 ttctgcagca agtgttgatc aaccaatcaa ttctgatagc gtagaaatct cgggtggatt
    54241 taaaggacaa gaaggcgtta aaaaagcaaa acaaatcgcc gaattgttaa atgcaggttc
    54301 tttaccagtt gacttgaaag aaatttattc aaactcagta ggtgcccaat ttggtcaaga
    54361 tgcattagat aaaactgtgt ttgcatcgtt tattggtgta gcattaattt atttattcat
    54421 gcttggattc taccgattac ctggtttagt agcgattatt gcattgacaa cttatatcta
    54481 tctaacgtta gtagcattta atttcatttc cggggttcta actttaccag gattagcggc
    54541 gttggtatta ggtgtaggta tggctgtaga tgccaatatt atcatgtatg agcgtattaa
    54601 ggatgaactt cgaataggta gaacgataaa gcaagccttt tctaaagcaa acaaaagttc
    54661 attcctaaca atttttgatt ctaacttaac aacagttatc gccgcagcag tattattctt
    54721 cttcggtgaa agttcagtta aaggtttcgc gacaatgtta ttattaggta ttctaatgat
    54781 ctttgttaca gccgtgttct tatcaagatt cttattatca ttacttgttt catcaaatat
    54841 attcaaaaat caattttggt tatttggtgt taaaaagaat aaacgacatg atattaatga
    54901 aggtgtagat gttcatgacc ttaaaacttc attcgagaaa tggaattttg ttaaattagc
    54961 taagccatta attggagtaa gtattttaat tgtagttgtc ggtttagtta ttctttatat
    55021 cttcaagtta aacttaggaa ttgatttctc aagtggtaca cgcgtagatt tccaatctaa
    55081 acaagcaatt actcaacaaa aggtagagca agttgtaaaa gatagtggac ttaaagcgga
    55141 tcaaattcag atcaatggta aagacaataa agtagcaact gttcaattta aagacgattt
    55201 aacgcgtgct caagataata aattgagtga taatattaaa tcgaaattcg gtgacacacc
    55261 tcaaattaac actgtatcac caattattgg acaggaatta gcaaaaaatg caatgcttgc
    55321 attaatctat gcatcgatag gtatcatcat ctatgtatca ttacgatttg aatggcgcat
    55381 gggtctttca tctgtattgg cattattaca tgatgtattc attatagtag cgattttcag
    55441 tttatttaga attgaagtag atttaacatt tatcgccgct gtattaacaa ttgtcggtta
    55501 ttcaattaat gatacaatcg taacgtttga ccgtgtacgt gaaaacttac aaaaggttaa
    55561 agtgattacg acaacagaac aaattgatga tatcgttaat agatcaatta gacagacaat
    55621 gacacgttca attaatacag tattaacagt tattgtagta gtagttgcta tactattctt
    55681 cggtgctcct acgatattca actttacttt agcattattt atcggattga tttctggtgt
    55741 attctcttca atcttcattg ccgttccgct atggggaata atgaaaaaac gtcagttgaa
    55801 aaaatcgccg aaacacaaat tagttgtata taaagaaaag aaatcgaacg atgaaaagat
    55861 tttagtttaa aatgaattaa gcggtatgtg aaacaataaa gagttcacat accgcttttt
    55921 actttattta aatttaacaa aatatatata aataagttcc cgcattcatt tttaaaaaat
    55981 ggtataatta aagtcttgat tatttagata agtgcttttt atttaaagta attttagata
    56041 taatgtcttt tggaaatgag gcgtaaatat atatgattaa accgaagtat aagtggaaat
    56101 taactaaacc agctgaatac ataagtgatg aattaacatc aaaattaaag ttaacgccaa
    56161 tcgtaaaaaa aatattagaa agtaaatcga ttattgatga acaagcaata gaatccatca
    56221 tttcagatac agatatcaat catgatgcac tgcaattgag cgatatgact aagactattg
    56281 aaagaattaa aagagcgata gcgaatgatg agaaaatcct agtttatggt gattacgatg
    56341 ccgacggtgt taccgcaact acaattttag tgataacatt acaactttta ggtgcacaag
    56401 taggatggca tattcctaac cgctttaccg aaggatatgg accaaacgaa ctagcattta
    56461 gaaatgctca tgatgaaggc ataacattaa tcataactgt tgataatggc atacaaggtc
    56521 ataatgaaat taaaatggtt caagatttag gagttgatgt cattgtaact gatcatcatg
    56581 aaattggtag tacattacca gaagcatatg caatcgtaca tccaatgcat ccatcgttta
    56641 attatccgtt ccaacaattg tgtggtgcag gtgttgcata caaattagca caagcattga
    56701 ttgaaaatgt acctgattat tttaaagcat tagttgcgat aggtaccatt gcggatttag
    56761 tatccttaac tgatgaaaat cgatcattag tgaaacaagg attaaaagtc ttaaatgatc
    56821 aatgtccgac ctctgttaaa gcattgctaa aagaagcggg atacaatgac aatattgatg
    56881 aagaaacaat tggttttatt attggaccta gacttaatgc tgtaggtcgt ttagatgacg
    56941 catcattagc ttgcgaattg ttgatgactg acgtcgaaga agaagctgca tttttagcag
    57001 aacaagttga acattttaac cgtgaaagaa aagatattgt agcaactatc acagaagaag
    57061 caatggctat ggcagaaacg aaagttaaaa agggagattt atttttactt ttagcaaaag
    57121 aaaattggca tgaaggtgtc ttaggtattg ttgcttcgaa aattgtcgaa acatttgctt
    57181 tgccaacatt aattttaaat attgataggg agcaaaatca tgcaaaaggc tccgccagat
    57241 caattgatca agtgtcgatg tttgaaatac tatcagcaca tcaagagtta atcgctaaat
    57301 ttggcggcca tcatatggct gcaggtatga cgatggatat tgaaaatata gaatcgttag
    57361 ctgaaggttt aaataaatgg atgaaagaat tatctaaaac aacttcgtta gatcccgtaa
    57421 agcaagtaga tgtattatta acagaaaatg atataacgat taaaaatatt cgtgatatga
    57481 atcgtttaag acccttcgga acagattttt ctagacctat ttttgaaatg gatgatttat
    57541 cagtttcttc agttaaggcg ataggtcaac aaaaaaatca tctaaaattg acacttggtg
    57601 aaagtaatat cgccgcgcta ttttggcaaa atggacactt agaacctgag ttacaagatg
    57661 aacagccaat taatatatta ggatctgttc aaatcaacga atggaatggt aatcaatcac
    57721 cgcaaataat tattcaagat attgcgatga atgaacagca aatattagat tatagaagta
    57781 agcgaaaaag tttacctttt acagaaaatg atgaaaatat tgtcgtgctt attcatccta
    57841 aaagtgataa agtaaatgcg aatgaatatt attatggtga agaaattaaa caacaaactg
    57901 ataaagtagt attaagagat ttaccaacgt caatggaaga cttgtctaat tccttgcaac
    57961 aactgcaatt ttctcaactt tatatagttt tgcaacataa tcattcgatt tacttcgatg
    58021 gtatacctaa tatggatatt tttaaaaagt gttataaagc attaataact aaacaagaaa
    58081 caaatatcca gaaagagggt atgttattgt gtcaacattt aagtgtgaaa ccagatacac
    58141 ttaaattcat gttgaaagtt ttcttagact taaaatttgt aacacaagaa gatggtttaa
    58201 ttcgaatcaa tcaacaacct gataaaagat cgattgattc cagcaaagta tatcaattaa
    58261 gacaacaacg tatggatgtt gaaaagcaat tattatatca agatttttca gaaataaaaa
    58321 attggataaa gtcacaattg tcgtgagcaa tttaggagga aatattaatg gatttaaagc
    58381 aatacgtatc agaagttcaa gattggccga aaccaggtgt tagtttcaag gatattacta
    58441 caattatgga taatggtgaa gcatatggct atgcaacaga taaaattgta gaatacgcaa
    58501 aagacagaga tgttgatatc gttgtaggac ctgaagcgcg tggctttatc attggctgtc
    58561 ctgtagctta ttcaatgggg attggctttg cacctgttag aaaagaaggg aaattacctc
    58621 gtgaagtcat tcgttatgag tatgacctag aatatggtac aaatgtttta acaatgcaca
    58681 aagatgcaat taaaccaggt caacgtgtgt taattacaga tgatttatta gctactggtg
    58741 gtacgattga agcagcaata aaattagttg aaaaattagg cggtatcgta gtaggtattg
    58801 catttataat tgaattgaaa tatttaaatg gtattgaaaa aattaaagat tacgatgtta
    58861 tgagtttaat ctcatacgac gaataataaa taatataatt ttatcaaatg aaatccttca
    58921 tcaaatgtat aagaaccaat gacttaatta aaaaagttgt ttaagttttc ttaacatgag
    58981 atgttaggat tttttattta ctgaaaatgt tagatgattg agcattatac cttaataaca
    59041 tcgtttattt atttcataaa ttgtagtatc atagaactaa tatttaaaaa atgaaacagt
    59101 agatttaggt cgaatttttg taaaagtttt aaaagtagga atagtataca aattaaactc
    59161 gctcaagtaa aattaatatt acgattaatg acgacaggat aaatatttat cgtcgacgga
    59221 cgtatgattg gtgtgggaca aatactattc aacaagagta cctaaatcat tgtttaaggc
    59281 gaagtaataa atatgaatgg ggtgtatcat ataatgaaca acgaatatcc atatagtgca
    59341 gacgaagttc ttcacaaagc aaaatcatat ttgtcagcag atgaatatga gtatgtttta
    59401 aaaagctatc atattgctta tgaagcacat aaaggtcagt tccgaaaaaa cggattacca
    59461 tacattatgc atcctataca agttgcaggt attttaacag aaatgcgatt agacggaccg
    59521 acgattgtcg caggtttttt gcatgatgta attgaagata caccgtatac atttgaagat
    59581 gtaaaagaaa tgttcaatga agaagttgct cgaattgttg atggtgtgac gaagcttaaa
    59641 aaagtaaaat accgctcaaa agaagaacaa caagctgaaa atcatcgcaa gttatttatt
    59701 gcgattgcca aagatgtacg cgtaattttg gtgaaattag cagacagatt acataatatg
    59761 cgtaccttga aagccatgcc gcgcgaaaaa caaattagaa tttctcgaga aacattagaa
    59821 atttatgcac cattagcaca tcgtcttggt attaatacaa tcaaatggga actagaagat
    59881 acggctcttc gttatattga taatgtgcaa tattttagaa tagtcaattt aatgaagaag
    59941 aaacgtagtg aacgtgaagc gtatatcgaa acggctattg atagaatacg tactgaaatg
    60001 gaccgaatga atatcgaagg cgatataaat ggtagaccta aacatattta cagtatttat
    60061 cggaaaatga tgaagcagaa aaaacaattt gatcaaattt ttgatttgtt ggcgatacgt
    60121 gttattgtca attctattaa tgattgttat gcgatacttg ggttggtgca tacgttatgg
    60181 aaaccgatgc caggacgttt taaagattat attgcaatgc ctaaacaaaa tttgtatcag
    60241 tcattgcata ctacagtagt aggcccaaat ggagacccgc tcgaaatcca aatacgaacg
    60301 tttgatatgc acgaaattgc tgagcatggt gttgcagcac actgggctta caaagaaggt
    60361 aaaaaagtaa gtgaaaaaga tcaaacttat caaaataagt taaattggtt aaaagaatta
    60421 gctgaagcgg atcatacatc gtctgacgct caagaattta tggaaacctt aaaatatgac
    60481 ttacagagtg acaaagtata cgcatttacc ccagcgagtg atgttattga gttgccatat
    60541 ggtgctgtgc cgattgattt tgcttatgcg attcacagtg aagtaggtaa taagatgatt
    60601 ggtgccaagg tgaatggcaa aattgtacca attgactata ttttacaaac aggcgatatt
    60661 gttgaaatac gtactagtaa acattcatat ggaccaagtc gtgattggtt gaaaattgtt
    60721 aaatcgtcta gtgccaaagg taaaattaaa agtttcttca aaaaacaaga tcgttcatct
    60781 aatattgaaa aaggccgaat gatggttgaa gctgaaataa aagagcaagg atttagagtc
    60841 gaagatattt tgacagagaa aaatattcag gttgttaatg aaaaatataa ctttgcaaat
    60901 gaagatgatt tattcgcagc tgtaggattt ggcggcgtga catccttaca gattgttaat
    60961 aaattaactg aaagacaacg tattttagat aaacaacgtg ctttaaatga agcacaagaa
    61021 gttacgaaat cattgcctat taaagacaac atcattactg atagtggtgt ctatgtagaa
    61081 ggtttagaaa atgtacttat caagttgtca aaatgttgta atcctatacc aggtgatgat
    61141 attgtaggtt atatcaccaa aggtcacggt attaaagtac atcgcactga ttgcccaaat
    61201 attaagaacg aaactgaacg actaattaat gttgaatggg taaaatcaaa agacgcaact
    61261 caaaaatatc aggttgattt agaggtaact gcgtatgacc gaaatggctt gttgaatgaa
    61321 gtactacaag ctgttagctc gacagccggc aatttaatta aagtttcagg acgttcagat
    61381 attgataaaa atgcaataat aaatattagt gtcatggtga aaaacgtgaa tgatgtttat
    61441 cgtgtggtag aaaagatcaa acaacttggt gatgtttata cagtaacaag agtttggaac
    61501 tagaggtgca aaatatgaaa gtagttgtac aaagagttaa agaagcatcg gtgacgaatg
    61561 atacattaaa taatcaaatc aaaaaaggat attgtttatt agtcggtatc ggtcagaact
    61621 ctacagagca agatgcagat gtaattgcaa agaaaattgc taatgcaaga ttatttgaag
    61681 atgacaataa taaattaaac tttaatatcc aacaaatgaa tggtgaaata ctatcagttt
    61741 cacaatttac tctctatgca gatgtaaaaa aaggtaaccg tccaggtttc tcaaattcta
    61801 aaaatcctga tcaagcggta aaaatttatg agtattttaa tgatgcgcta cgagcgtatg
    61861 gtcttactgt gaaaacaggt gaatttggaa cacacatgaa tgttagcata aataatgatg
    61921 gtccagtcac tattatttat gaaagtcagg acggcaaaat tcaatgaaaa aaatagaggc
    61981 atggttatct aaaaagggtc ttaaaaataa acgtactcta atagtagtga ttgcctttgt
    62041 cttatttatc atctttttat ttttattgct gaatagcaat agtgaagata gtgggaacat
    62101 cacgataact gaaaatgctg aattacgtac aggtccaaac gctgcgtatc cagtcatata
    62161 taaagttgaa aaaggtgacc attttaaaaa gattggtaaa gtaggtaaat ggattgaagt
    62221 tgaagataca tccagtaatg aaaaaggttg gatagctgga tggcacacaa atttagatat
    62281 tgtcgcggat aatacgaagg agaaaaatcc tttgcaaggt aaaacaatag tgcttgatcc
    62341 tggtcatgga ggtagtgacc agggtgcttc aagcaatact aaatataaaa gtttagaaaa
    62401 agattatacg ttgaaaacag caaaagaatt gcagcgtact ttagaaaaag aaggcgcaac
    62461 tgttaagatg acaagaacag acgatacata tgtttcacta gaaaatcgtg atatcaaagg
    62521 cgatgcctat ttgagtatac ataatgatgc gttagaatca tctaatgcaa atggaatgac
    62581 agtttattgg tatcatgata atcaaagagc tttagcagat acgttagacg ctacgattca
    62641 gaagaaaggt ctactttcta atcgcggttc aagacaagaa aattatcaag tgttaagaca
    62701 aacaaaagtt cctgctgttt tattagaatt aggttatatt agtaacccaa ctgatgaaac
    62761 gatgattaaa gatcaattac atagacaaat tttagaacaa gcaattgttg atggccttaa
    62821 aatttatttt tctgcgtagg gcttgcaaaa atatgtgaaa gtagttatca ttgatattga
    62881 attttataac taaaaccgtt agtattcttg aaatggtaaa tgaaataggt agcaatctaa
    62941 ctaagattgt gtaggaatat aatccataga ctgaaagatt atgctgagta gtttatatac
    63001 attgaacaca agaagaggtg ctttatgaaa agtaaagccg ttaaacgtac gttaaacgtt
    63061 ttgagtgggt ttattaaatg cacgcttata aaaagtaatg atgattacaa ttaggcatgt
    63121 tttttaaacc aatgagggtg gcaacacgga tattcgtccc ttgtgcagtt tcaggctgtg
    63181 caagggcttt ttcttttgct tttaagatta tgatttatcg tgcaaagtta agtggtcgta
    63241 tatagtttta gttttaaaaa ggtaattaaa taaaatagtt tgccgaggga gatgtcaaaa
    63301 tgattaaaat acctagaggg acgcaggata ttttacctga agattcaaag aaatggcgtt
    63361 acattgaaaa tcaattagat gaattaatga cattttataa ttataaagaa ataagaacac
    63421 caatttttga aagtacagat ctttttgcaa gaggtgttgg tgattcaacc gatgtcgtac
    63481 aaaaagaaat gtatacattt aaagataaag gcgatagaag tattacatta agacctgagg
    63541 gaacagctgc agttgtgcgt tcatatattg aacataaaat gcaaggtaat ccaaaccaac
    63601 caattaaact ttattacaat ggaccgatgt ttagatatga acgtaagcaa aaaggacgct
    63661 atcgtcaatt taatcaattt ggtgtagaag ctattggtgc tgaaaatcct agcgtagatg
    63721 cagaagtatt agctatggtt atgcatattt atcaatcatt tggattaaaa catttaaagc
    63781 ttgttattaa tagtgtaggg gatatggcgt ctcgaaaaga atataacgaa gcgttagtga
    63841 aacactttga accagtaatt catgaatttt gttcagattg tcaatcacgt ttgcatacaa
    63901 atccgatgcg aattttggat tgtaaagtag accgtgataa agaagcgatt aagactgcac
    63961 ctagaatcac tgatttctta aatgaggaat ctaaggcata ttatgaacaa gtaaaagctt
    64021 atttagatga tttaggtatt ccatatattg aagatcctaa cttagttcgt ggattggatt
    64081 attatacaca tacagcattt gaattaatga tggataaccc taactatgat ggtgccatta
    64141 caacgctttg tggtggtggc cgttataatg gtttattaga attgctagat ggtccaagtg
    64201 aaacaggtat tggttttgcg ctaagtatag aacgattatt gcttgcactt gaagaagaag
    64261 gtatcgaatt agatattgaa gaaaacttag atttattcat tgttacaatg ggtgatcaag
    64321 cagatcgata tgctgtgaag ctattaaatc atttgagaca taatggtatt aaagcagata
    64381 aagactattt acagcgtaaa attaaaggac aaatgaaaca agcagaccgt ttaggtgcca
    64441 agtttacaat cgttattggt gatcaagaat tagaaaataa taaaatcgat gttaaaaata
    64501 tgacaactgg tgaatctgaa acaattgaat tagacgcatt agtcgaatat tttaagaagt
    64561 agagagggcg ttaaaatatg agtaagagaa caacttattg tggattagtt actgaggcat
    64621 ttttaggaca agaaattaca ttaaaaggat gggttaacaa tcgtcgtgac cttggtggat
    64681 tgattttcgt tgatttaaga gatagagaag gaattgtaca agtcgtgttt aatcctgcat
    64741 tttcagaaga ggcattgaaa attgctgaaa cagtacgttc tgaatatgtt gtagaagttc
    64801 aaggtacagt tacgaagcgt gaccctgaaa cagttaatcc taaaattaaa actggccaag
    64861 ttgaagtaca agttacaaat attaaagtga ttaataaatc tgagacacca ccattttcta
    64921 taaatgaaga aaatgttaac gttgatgaaa atattcgatt aaaataccgt tatttagatt
    64981 tacgtcgtca agagttagcg caaacattta aaatgagaca tcaaattaca cgttctattc
    65041 gtcaatattt ggatgatgaa gggttctttg acatcgaaac accagtacta acgaagtcaa
    65101 cacctgaggg tgcacgtgac tatttagtac catctcgtgt tcatgatggt gaattttatg
    65161 cattaccaca atcaccacaa ttatttaagc aattattgat gattagtgga tttgacaaat
    65221 actaccaaat cgtaaaatgc ttccgtgacg aagatttacg tgcagatcgt caacctgaat
    65281 ttacacaagt cgatattgaa atgagttttg tagaccaaga agatgtgatg caaatgggtg
    65341 aagaaatgct taaaaaagtt gttaaagaag ttaaaggcgt tgaaattaat ggcgctttcc
    65401 cacgcatgac atataaagaa gcgatgcgtc gctatggttc tgataaacca gatacacgtt
    65461 ttgaaatgga attaattgac gtttctcaat taggacgtga tatggacttt aaagtattta
    65521 aagatactgt tgaaaatgat ggtgaaatta aagcaattgt cgctaaaggt gcagctgaac
    65581 aatatactcg taaagatatg gatgctttaa cagaatttgt aaacatctat ggtgctaaag
    65641 gattagcgtg ggttaaagtt gtggaagatg gtttgacagg tccaattgga cgtttctttg
    65701 aaacagaaaa tgttgaaaca ttacttacat taactggtgc tgaagctggt gacttagtaa
    65761 tgtttgttgc agacaaacca aatgtcgttg cacaaagttt aggtgcatta cgtgtcaaat
    65821 tagctaaaga attaggttta atcgatgaaa caaaattaaa cttcttatgg gtgacagatt
    65881 ggccattatt agaatatgat gaagatgcga aacgttacgt tgcagcacat catccattta
    65941 catctccaaa agaagctgat attgctaagc ttggcactgc gccagaagaa gctgaggcaa
    66001 atgcttatga catagtatta aatggttatg aattaggtgg cggttcaatc agaattcatg
    66061 atggtgagtt acaagaaaaa atgttcgaag ttcttggatt tactaaagaa caagcacaag
    66121 aacagttcgg cttcttacta gatgcattta aatatggtgc accaccacat ggcggtattg
    66181 cattaggatt agaccgatta gtcatgttat tgactaatag aacaaacttg agagatacaa
    66241 tcgcattccc taaaacagca tctgctacat gtttattaac gaatgcgcct ggtgaagttt
    66301 ctgataaaca attagaagaa ctttctttgc gaattcgtca ctaaatttta aaaaatcaat
    66361 aaactttggt ttgttataat ttcattaatt ttaattatgt ggtaatataa catcataaga
    66421 tagtcatctg agttgttcgt aagtttgctt tttaatttgg gcctaacact ctttgatcaa
    66481 gggagcccaa tgggttttct tgcagcgtac acgccttata tgaaggacgt acaaaacaag
    66541 aaacagggca cccacctgta tataacaggc cgaatgatca agctattata actacggcaa
    66601 tacggactct atcgatacgc aagactttgt cttgcgtatt tttatgcgtt tctttaagtt
    66661 tttaataaag aattagttgg gttttatagc gtttaataaa tgtttcctcg ttgaattttg
    66721 ggaaaataat caaagtgata atgctaaata taaaatttta agttactatg tacttatgta
    66781 taaatcagtg aaagaggtaa aataatgaaa catcaatttt caagaaatga actagcgatt
    66841 ggacaacaag gtttagattt attgaagcaa caaactgttg tcatacttgg cgttggaggt
    66901 gttggttcat ttgctgctga agcattagca agaacgaata ttggtcatat tatattaata
    66961 gataaagatg atgttgatat tacaaatgta aacagacaat tacacgcttt aactacaacg
    67021 gtaggacaaa gtaaagtttc tttgatggaa gaacgtatta aattaattaa tccagattgt
    67081 aaagtcacgc ccttacatat gttttataca gaagagacat atgaagaaat tttcgataac
    67141 tacgatatag attattttgt agatgcaagt gatacaataa tgtacaaagt acacttaatg
    67201 aaagagtgtc ttgaccgagg gattaaagtg atttccagta tgggtgctgc aaataaaaca
    67261 gatccaacac gttttacaat ttcagatatc tctaaaacac ataccgatcc aatggccaaa
    67321 attattcgtc aaaaattgaa aaaactagga attcgtaaag ggattccagt tgtattttca
    67381 gatgaaagtc caattgtcat aagagaagat gtaaaagata tagttggaga taaaaatgct
    67441 atcaatcgaa aaggacaaat gccaccttct tcaaatgcct ttgtgccaag tgttgttgga
    67501 ttaatttgtg caagttatgt ggtgaatgac gtattaaaag atattccagt tcgtcgcatt
    67561 aaagacaaag ggcaataatt cattttgaaa gggataattt tcaacgtaag gcaagtgtaa
    67621 ccacaccata aaactaaaac tgactagttc gcaagcatag tagaatatgc ctcgtgtact
    67681 agtcagtttt gatttgatta tagctaaaat atcaaaggta atcgatttaa tcttattttt
    67741 acggcctttg tttaagtaag ttgttatata tttctttgag ttgttgttca cttttagatg
    67801 tcgtttttgg ttcgtaataa attttgtttt ttagtttatc aggtaaatat tgctgtgaaa
    67861 catagccatt aacatattgg tgtggatatt tgtaaccaat agatcggcct agatctttag
    67921 caccttgata atgtccatct tttaaatggt ttggaatttg gcccacatga ccgtttctaa
    67981 tatcggacaa tgcactatca atggcactca ttgctgaatt tgacttaggt gataagcata
    68041 gttcgattac tgcttggctt agtggaattc tagcttctgg taaacctaga cgttctgctg
    68101 attcaatagc agcaagtgtt ctctgaccag cattaggtga ggctaagcct atatcctcat
    68161 agctaattac aagtaatcgt cgaactattg taggtaaatc tccagcttca attaatcgtg
    68221 ctaaataatg taaagcggca ttgacgtcgc taccacggat agatttttgg aaagcgctca
    68281 taacatcgta atgcatgtca ccatccttgt cacttacaaa tgcacctttt tgtaaacagt
    68341 ctttagcatc ttgcaatgta acatgtcgat aaccgtcttt gtcattatct gcgcttaata
    68401 cagctaattc caatgcattt aacgcactac gaacatcacc ttggctttgt gtagaaaagt
    68461 aggtcatggc atcttcatca attttgggtt gatatgtttt caaaccattc tcatcatctt
    68521 ctattgcacg agttaacgct tggcgcacat cttcgtcatt taaaggatat aactcgaaaa
    68581 tttgcgctct tgaacgaatc gctggattga tagcatgata aggatttgaa gttgtagcac
    68641 cgatcaagac gattttgcca ttttctaaat gaggtaataa aaagtcttgt ttagctttat
    68701 ctagtcgatg tatttcatct aataacaaga taacttgacc agacatttta gcttcttcaa
    68761 caacaagttg catatctttt ttagtgttag ttacagcatt caattgtctg aatttatatt
    68821 gcgtactgcc cgaaattgct ttggcaatac ttgttttgcc tataccaggt ggaccataaa
    68881 aaatcattga agttaatttt tttgtatcaa ccattcttct gataatgcct cttggtccaa
    68941 ctaaatgttg ttgggaaatg atttcatcta tattttttgg gcgcattctc gatgctaatg
    69001 gttctgtact cacatttgtc acacctttct tatactatct taactcaaaa aatgctaaaa
    69061 tgggaatatc aatattcgaa ttaagtgagg ttatacaatg aaaatttcta ctaaagggag
    69121 atatggactt acattgatga tttctcttgc taaaaaagag gggcaaggat gtatatcatt
    69181 aaagtcaatt gctgaagaaa ataatttgag tgatttatat ttagaacagc ttgtaggtcc
    69241 tttaagaaat gcggggttaa ttcgaagtgt acgcggtgct aaaggtggat accaattaag
    69301 agtgccagcg gaagaaatct cagcagggga tattataaga ctgttagaag gtccaattac
    69361 atttgttgaa agtattgaat cagaaccacc tgcgcaaaaa caactatgga ttcgcatgag
    69421 agatgcagtg agagatgttt tagataatac aacattgaaa tatttagcgg aatatgtaga
    69481 tacaagtgaa gatttagacg gatacatgtt ttatatttaa tcgtataaat actatactat
    69541 aacataaaaa cttcatatta taatgtttag cgaacctcct tagtggtata taaatatata
    69601 catccaagga ggtttcacta tggcagacga aagtaagttc gatcaattta aaggaaatgt
    69661 taaagaaaca gtaggtaacg ttactgataa caaagaatta gaaaaagaag gtcagcaaga
    69721 taaagtgatt ggtaaagcaa aagaagttgt tgaaaatgct aaaaacaaaa taactgatgc
    69781 aattgataaa cttaaaaaat aagttcaatt aaactaatca aatataagag gagcgattaa
    69841 tatgagtttt atggataaag ctaaagacgc agtagaaaaa tttaaaaata gcgataatga
    69901 acaagttaaa aatgtaaaag ataagataaa tgaatataca ggatcgaata acgaagagaa
    69961 aaaagaaaat gaagataaag agaaataaat ttctttaaat tttggatatg cttttcaata
    70021 ttttgaaagc gccccatcaa ataaacactt acacagtgta aagttgaagg ggcgctttca
    70081 tatttgcatt agaattttaa gtgaatttga atcattacta aaataaatga ttgaacaata
    70141 aagacatctt tcattcaaaa tgctg
//
LOCUS       DALAAR010000004        66150 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000004 DALAAR010000000
VERSION     DALAAR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 66150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.004"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            34..240
                     /gene="copZ"
                     /locus_tag="O2458_000240"
     CDS             34..240
                     /gene="copZ"
                     /locus_tag="O2458_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCZ3886130.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(330..1328)
                     /locus_tag="O2458_000241"
     CDS             complement(330..1328)
                     /locus_tag="O2458_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ3886131.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(1351..2505)
                     /locus_tag="O2458_000242"
     CDS             complement(1351..2505)
                     /locus_tag="O2458_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ3886132.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(2932..4440)
                     /locus_tag="O2458_000243"
     CDS             complement(2932..4440)
                     /locus_tag="O2458_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3886133.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(4452..5315)
                     /locus_tag="O2458_000244"
     CDS             complement(4452..5315)
                     /locus_tag="O2458_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCZ3886134.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(5352..6479)
                     /gene="crtQ"
                     /locus_tag="O2458_000245"
     CDS             complement(5352..6479)
                     /gene="crtQ"
                     /locus_tag="O2458_000245"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCZ3886135.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(6485..7978)
                     /locus_tag="O2458_000246"
     CDS             complement(6485..7978)
                     /locus_tag="O2458_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3886136.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(7971..8459)
                     /gene="crtO"
                     /locus_tag="O2458_000247"
     CDS             complement(7971..8459)
                     /gene="crtO"
                     /locus_tag="O2458_000247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCZ3886137.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(8634..9401)
                     /locus_tag="O2458_000248"
     CDS             complement(8634..9401)
                     /locus_tag="O2458_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3886138.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(9762..11573)
                     /locus_tag="O2458_000249"
     CDS             complement(9762..11573)
                     /locus_tag="O2458_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ3886139.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(12084..12571)
                     /locus_tag="O2458_000250"
                     /pseudo
     CDS             complement(12084..12571)
                     /locus_tag="O2458_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(12847..13548)
                     /gene="isaA"
                     /locus_tag="O2458_000251"
     CDS             complement(12847..13548)
                     /gene="isaA"
                     /locus_tag="O2458_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCZ3886140.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(14156..15211)
                     /locus_tag="O2458_000252"
     CDS             complement(14156..15211)
                     /locus_tag="O2458_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ3886141.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVLPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(15338..15466)
                     /locus_tag="O2458_000253"
     CDS             complement(15338..15466)
                     /locus_tag="O2458_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886142.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            15621..16190
                     /locus_tag="O2458_000254"
     CDS             15621..16190
                     /locus_tag="O2458_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3886143.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            16388..16888
                     /locus_tag="O2458_000255"
     CDS             16388..16888
                     /locus_tag="O2458_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886144.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            17108..17338
                     /locus_tag="O2458_000256"
     CDS             17108..17338
                     /locus_tag="O2458_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ3886145.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(17488..17865)
                     /locus_tag="O2458_000257"
     CDS             complement(17488..17865)
                     /locus_tag="O2458_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886146.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(17882..18703)
                     /locus_tag="O2458_000258"
     CDS             complement(17882..18703)
                     /locus_tag="O2458_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCZ3886147.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(18723..19022)
                     /locus_tag="O2458_000259"
     CDS             complement(18723..19022)
                     /locus_tag="O2458_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCZ3886148.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            19153..19710
                     /locus_tag="O2458_000260"
     CDS             19153..19710
                     /locus_tag="O2458_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3886149.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            19703..20407
                     /locus_tag="O2458_000261"
     CDS             19703..20407
                     /locus_tag="O2458_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ3886150.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            20505..21515
                     /locus_tag="O2458_000262"
     CDS             20505..21515
                     /locus_tag="O2458_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ3886151.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            21535..22365
                     /locus_tag="O2458_000263"
     CDS             21535..22365
                     /locus_tag="O2458_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3886152.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(22512..23402)
                     /locus_tag="O2458_000264"
     CDS             complement(22512..23402)
                     /locus_tag="O2458_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ3886153.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(23500..24840)
                     /locus_tag="O2458_000265"
     CDS             complement(23500..24840)
                     /locus_tag="O2458_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCZ3886154.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(24834..25940)
                     /locus_tag="O2458_000266"
     CDS             complement(24834..25940)
                     /locus_tag="O2458_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCZ3886155.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(26274..26570)
                     /locus_tag="O2458_000267"
     CDS             complement(26274..26570)
                     /locus_tag="O2458_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCZ3886156.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            complement(26558..26926)
                     /locus_tag="O2458_000268"
     CDS             complement(26558..26926)
                     /locus_tag="O2458_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886157.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(26941..27219)
                     /locus_tag="O2458_000269"
     CDS             complement(26941..27219)
                     /locus_tag="O2458_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCZ3886158.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            complement(27561..28427)
                     /locus_tag="O2458_000270"
     CDS             complement(27561..28427)
                     /locus_tag="O2458_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCZ3886159.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            28645..29709
                     /locus_tag="O2458_000271"
     CDS             28645..29709
                     /locus_tag="O2458_000271"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCZ3886160.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            29864..30139
                     /locus_tag="O2458_000272"
     CDS             29864..30139
                     /locus_tag="O2458_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886161.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(30325..31047)
                     /locus_tag="O2458_000273"
     CDS             complement(30325..31047)
                     /locus_tag="O2458_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCZ3886162.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            31401..31850
                     /locus_tag="O2458_000274"
     CDS             31401..31850
                     /locus_tag="O2458_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCZ3886163.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(31931..32518)
                     /locus_tag="O2458_000275"
     CDS             complement(31931..32518)
                     /locus_tag="O2458_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3886164.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(32496..32594)
                     /locus_tag="O2458_000276"
     CDS             complement(32496..32594)
                     /locus_tag="O2458_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886165.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            32651..34333
                     /locus_tag="O2458_000277"
     CDS             32651..34333
                     /locus_tag="O2458_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCZ3886166.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(34462..34845)
                     /locus_tag="O2458_000278"
     CDS             complement(34462..34845)
                     /locus_tag="O2458_000278"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCZ3886167.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(34847..35698)
                     /locus_tag="O2458_000279"
     CDS             complement(34847..35698)
                     /locus_tag="O2458_000279"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCZ3886168.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(35691..36509)
                     /gene="panB"
                     /locus_tag="O2458_000280"
     CDS             complement(35691..36509)
                     /gene="panB"
                     /locus_tag="O2458_000280"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCZ3886169.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            36582..37442
                     /locus_tag="O2458_000281"
     CDS             36582..37442
                     /locus_tag="O2458_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ3886170.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(37528..38232)
                     /gene="budA"
                     /locus_tag="O2458_000282"
     CDS             complement(37528..38232)
                     /gene="budA"
                     /locus_tag="O2458_000282"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ3886171.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(38510..39469)
                     /locus_tag="O2458_000283"
     CDS             complement(38510..39469)
                     /locus_tag="O2458_000283"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ3886172.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            40156..41604
                     /locus_tag="O2458_000284"
     CDS             40156..41604
                     /locus_tag="O2458_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3886173.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(41685..43022)
                     /locus_tag="O2458_000285"
     CDS             complement(41685..43022)
                     /locus_tag="O2458_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCZ3886174.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            43280..43696
                     /locus_tag="O2458_000286"
     CDS             43280..43696
                     /locus_tag="O2458_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886175.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            43819..44709
                     /locus_tag="O2458_000287"
     CDS             43819..44709
                     /locus_tag="O2458_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCZ3886176.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(44902..46398)
                     /gene="mqo"
                     /locus_tag="O2458_000288"
     CDS             complement(44902..46398)
                     /gene="mqo"
                     /locus_tag="O2458_000288"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ3886177.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            46694..47023
                     /locus_tag="O2458_000289"
     CDS             46694..47023
                     /locus_tag="O2458_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886178.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(47083..48681)
                     /locus_tag="O2458_000290"
     CDS             complement(47083..48681)
                     /locus_tag="O2458_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ3886179.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(48875..49318)
                     /locus_tag="O2458_000291"
     CDS             complement(48875..49318)
                     /locus_tag="O2458_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCZ3886180.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(49599..49811)
                     /locus_tag="O2458_000292"
     CDS             complement(49599..49811)
                     /locus_tag="O2458_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ3886181.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(50089..51798)
                     /gene="betA"
                     /locus_tag="O2458_000293"
     CDS             complement(50089..51798)
                     /gene="betA"
                     /locus_tag="O2458_000293"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCZ3886182.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(52059..53549)
                     /gene="betB"
                     /locus_tag="O2458_000294"
     CDS             complement(52059..53549)
                     /gene="betB"
                     /locus_tag="O2458_000294"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCZ3886183.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            53618..53713
                     /locus_tag="O2458_000295"
     CDS             53618..53713
                     /locus_tag="O2458_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886184.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            53802..54365
                     /locus_tag="O2458_000296"
     CDS             53802..54365
                     /locus_tag="O2458_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCZ3886185.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(54553..55185)
                     /locus_tag="O2458_000297"
     CDS             complement(54553..55185)
                     /locus_tag="O2458_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ3886186.1"
                     /translation="MTKNLYATPTSELPFDNRFLFKSFILKRETGNIVIYHSGHLGDS
                     QQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVAALKHKISVQKQFSNRVML
                     DKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGKRWRTVILGSSDREKSIQS
                     LEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNIIDRVREGENS"
     gene            complement(55275..56897)
                     /locus_tag="O2458_000298"
     CDS             complement(55275..56897)
                     /locus_tag="O2458_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ3886187.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            57029..57190
                     /locus_tag="O2458_000299"
     CDS             57029..57190
                     /locus_tag="O2458_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886188.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(57206..57316)
                     /locus_tag="O2458_000300"
     CDS             complement(57206..57316)
                     /locus_tag="O2458_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886189.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(57416..57952)
                     /gene="nrdG"
                     /locus_tag="O2458_000301"
     CDS             complement(57416..57952)
                     /gene="nrdG"
                     /locus_tag="O2458_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCZ3886190.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(57949..59799)
                     /gene="nrdD"
                     /locus_tag="O2458_000302"
     CDS             complement(57949..59799)
                     /gene="nrdD"
                     /locus_tag="O2458_000302"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCZ3886191.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(60046..61416)
                     /locus_tag="O2458_000303"
     CDS             complement(60046..61416)
                     /locus_tag="O2458_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCZ3886192.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(61738..61833)
                     /locus_tag="O2458_000304"
     CDS             complement(61738..61833)
                     /locus_tag="O2458_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886193.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(61909..62514)
                     /locus_tag="O2458_000305"
     CDS             complement(61909..62514)
                     /locus_tag="O2458_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCZ3886194.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(62577..64457)
                     /locus_tag="O2458_000306"
     CDS             complement(62577..64457)
                     /locus_tag="O2458_000306"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCZ3886195.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            64652..64744
                     /locus_tag="O2458_000307"
     CDS             64652..64744
                     /locus_tag="O2458_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886196.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(64852..65349)
                     /locus_tag="O2458_000308"
     CDS             complement(64852..65349)
                     /locus_tag="O2458_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ3886197.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(65359..65619)
                     /locus_tag="O2458_000309"
     CDS             complement(65359..65619)
                     /locus_tag="O2458_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886198.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(66001..66114)
                     /locus_tag="O2458_000310"
     CDS             complement(66001..66114)
                     /locus_tag="O2458_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886199.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT        21024 a        12456 c         9973 g        22697 t
ORIGIN      
        1 aataataagt ggctctaaaa ggaggtgaat gctatgtcac aagaaatttt aaatgttgaa
       61 ggtatgagct gtggtcactg caaaagtgct gttgaatctg cattaaataa tattgacggt
      121 gtcacttcag ctgacgttaa ccttgaaaat ggtcaagtaa gtgttcaata tgatgacagt
      181 aaagttgctg tatctcaaat gaaagacgca attgaagatc aaggttacga tgtcgtttaa
      241 ttaggcaata ttcaacgtca tcaacaccaa attaaaaaat cgaactgatg agaatcccaa
      301 caatccaaat tatctcatca gttcgatttt taatttactc gtaacctagt atctccagtc
      361 tgcaatacat ctaatgttgc atctaatgca tcgacaatta gatttttaac tgcagcttca
      421 gtataaaacg caatatgtgg tgttaatatg acatcttccc tgtcaatcaa cgattctaac
      481 aatggatcgt tcagtgtttt gcccctttga tcacttggga aaagtttgcg ttcaaattca
      541 tacgtatcaa gtgctgcacc tttaatcaca ccattgtcta atgcgtctaa taacgcctta
      601 gtatctacta aagaacctct cgcacaattg acaaatactg cgcccttttt aaaatgttta
      661 aataattcag cattaaatag ataatgatta tatttcgttg caggtacatg taatgtcacg
      721 atatcagcac cttcaaccgc ttcctcaatc gtatctttgt aatcgacata cgttgcaatt
      781 ttagcattag gaaacgggtc gtatgcgacc acatcacttt gataaccatt ggcaaatata
      841 tcggctacta cacggccaat tcgacctgta ccaataacag ctacttttaa atctttaatg
      901 gatttcgata aaatagtagg ttcccatcta aaatcatgct cccgcacttt cgtttgaatt
      961 tgattaaaat gacgaaccac attaatagcc tggttcacag caaactccgc aattgaattc
     1021 ggagagtatg acggcacatt tgacacaata aagttatact tgtttgctaa ctccaaatca
     1081 tatgtatcaa atccagcact acgttgtgcg atttgtttaa tacctagttc atttaatcgt
     1141 ttataaacat gctctgataa tggtatttgt tgtgatagcg ataagccatc ataaccagcg
     1201 acaccttcaa cattgtcatc agttaatgct tctttagtaa tatctacctc aacatgatgt
     1261 ttctctgccc acgccttgat ataaggcata tcttcatcac gtacactcat gattttaatt
     1321 tttgtcattt taacatcacc cttaacttta ttattcatat aaatatgcta gttctgttaa
     1381 tcttattgca gcttcgtcta atttctggtc atctaacgcc aatgaaattc tcacataacg
     1441 attaccattc tctccaaatg gtttccctgg agcaacaagt attgacttct cttgcactaa
     1501 aaattgctca aattgctcgc tgtcataacc aggcggtgtt tccaaccata catatatgcc
     1561 acctttagca tgaacaaatg gcaaatcagc ttttgcaagc atggcttcga atcggtcacg
     1621 acgtgtttta aatacattgc tttgttcttc taaaaaatca tcataatgat tcaaagcata
     1681 tattgcggca tcttgtaatg caccaaacat cccagcattt gtgtgcgttt ggtacttttt
     1741 caaagcttga atcatatctt tattaccaac tgcaaaaccg actctaaaac ctgacatgtt
     1801 ataaccttta gacaaggaat aaatttcaat cgcaacatct ttcccatttt ccgaagcaag
     1861 tatactagga tttttagcgt cgaaaccgaa agcaccataa gcaaaatcat gcacgatttt
     1921 agtgtctgta cctttaaatt tagctatcgc ttcatcaaaa acttctttcg tagctgtcga
     1981 tccagttgga ttatttggat acgttaaata aatgagtttt gttttatcta ttatttgtga
     2041 atcaactttg gaccaatctg gcaaataatg tggcggttct aaattaagcg ggactggctt
     2101 gccatcagct aaaagtacac ctgctaaata atccgtgtag cctggatcag gtagtaatac
     2161 atagtctcct ggattgataa cacatgttgg tactgccact aatccatttt ttgtaccata
     2221 taaaatgcat acttcatctt ctttatctaa cgtcacatta tattgtcttt gataaaaatc
     2281 tacaatagct tgcttgaacg cttctttacc atgaaaagca ccatattttt gattttcagg
     2341 aatagttagt gctttttgaa aatgatcaat aataccttgt ggcgtgggcc catcagggat
     2401 tccaactgcc atattaatta atggcaatgg tccatgttcg attttacgtc ccatcgtttt
     2461 cccgaaataa ctatcaggga tatttgctaa tttgttagag atcatcaaat tcctcctcta
     2521 tcattaaaca tagcctgggc gactatcata atcctaacaa cttgtatcac tctcatttag
     2581 atggttacaa tgacatcgcc attcaccgtt atgttcaaca gaacttatga cacacgttgt
     2641 attgaatgaa tttattttca ttttaggtag gtataatatt attgtcaata ttaggaattt
     2701 tcagattaat atgcactcaa tcgttatgat ttaactgtca tgcatatccg catgcgcaac
     2761 cagttagata tgcttatata aagtataacg cccatcaagg tacgtattca aacgtgaacc
     2821 ttaacaggcg tcattcattg ttaaataaaa cttcttaagc acatacttat ttcactatgc
     2881 cttttacgtt ccccttatac ttttctcaca tctttctctt agactactcc cttatacgcc
     2941 ccgctcaata tctttaatca tttcatctac agttattttc gcactcgtta agacaatagg
     3001 aacgcctgca cctggatgcg tacttgcacc tgcaaaatat aaatctttat aatctcgcga
     3061 tacattttgt ggacgataat aattactttg cgctaaagtt ggcattaaac cgaatgccga
     3121 accaaatttc gcatgatacg tttgctcaaa atcatttggc gtaaagattg tttctgaaac
     3181 aatatgcgat tttatatctt caaatacttc aatcgttgct aatttacgat aaataatttc
     3241 ctttatttgt tgcgtcaaag cttcatctga ccaatcgatt ccgctacctg ttttaagttc
     3301 cggcgtcggc attagcacat aaataccagt tttgccttct ggcgcaagtg atttatcagc
     3361 gaccgctggt acatacacat aaatagaagg atcatatgat aaacgtccct caaatatttc
     3421 ttcaatattg cctctaaagt catctgaaaa aataacatta tgaagtctca cttgatctgt
     3481 cacatcaata tctataccga tatacattaa aaatgctgaa caagagtaat ctaagtctgc
     3541 aattttatgt ggtggatact ttttaatagg tgcaaaatct ggcattaatg attccgcaac
     3601 actagggaaa tcagccgtac ataaaatttt atcaaatttt cttatgtcac cattcacttt
     3661 tatcgcatcg gcccgtttga atttaggatc aataataatt tgctcaattt cagcatttag
     3721 ttcaatatta acgcctaagt ctttatttaa ttgcgctagc ccttgagcca tgccatacat
     3781 accgccttta ataaaatgca caccaaacat catttcaatc ataggaataa ttgaatatag
     3841 tgacgggcct cgttttggat caattcctat gtataacgtt tgaaacgcta aaagcttttg
     3901 tatcttttcg ttatcaatat aatgttcaat tagctgatct gcatgattta acgtttttaa
     3961 cttagcacct tgcacaagtg acgtcatatt ataaaagtca ctcggtttgc gatacgttct
     4021 ttctaagaaa tagcgacgtg caatttcata ttttttataa acatccgtta aaaaggacat
     4081 aaaaccatgc gttgaaccag gttctatact ttctagcatt tgctgtaatt cagctaaatc
     4141 tgtaggcacc gttatacgat catcgtggtc aaaatacaca tcgtaaatat aacgtaattg
     4201 tctcaattca atataatctt cataattttt accacacgct gtaaaaacat ctttataaac
     4261 atctggcatc atgacaattg tgggacccat atcaaatgta aagccgtctt tctttaattg
     4321 attcatacgc ccgcctacat tattattttt ttcaaatatc gtcacttcat gaccttgaga
     4381 agcaatacgg gctgccgctg ctaatcctgt gacacctgca ccaattactg caatcttcat
     4441 tattcaacca cctatattct atgatattta ctatttattt catgaaacaa ctttgccttt
     4501 ttcctcttat ccacaaaaac acgttcatgt aatgtatagt tagcctgtct cacttcgtcc
     4561 agtatttcaa tatatatacg tgctgctaat tctatgattg gttgtgcttc aatactaaat
     4621 actttgattt gatccataac atcttgaaaa tctttttctg cgatagctgc ataatattcc
     4681 cataagtcaa tataatgatt attaacacca ttttggtaca cttcagcaat atcaacttca
     4741 tattgcttta atcgttgctt actaaaatat atccgttcat tgtcaaaatc ttcaccgaca
     4801 tctcttaata tattaatcaa ttgcaacgat tcaccaagtc ttcttgcgac atcgtatgtc
     4861 tgatgtgttt catgatcact taaaatcggc gtcaatactt cacctactgt accagcaaca
     4921 ccataacaat atccgaataa ttcagcgtcc gtttcaaaca ttgtaaaatg ttgatcttta
     4981 tatacagtat caatgagatt ataaaaagat tgaaaggcga tatttttatg ttgtgcaaca
     5041 tgctgaagcg ccatcatgat tctacgatca ctttgaaagt gatgatgttc atatgggtat
     5101 ttttcaatag attgtatatc ttcttttatt tgatttaaaa attgaatatc gccataaaca
     5161 tctatactgt catcaatttt acgacacaca gcataaattg cccaaaccgc ttttctttga
     5221 tcttctggta acaagtcaaa agcgtaagaa aagctttttg aatgtttctt catgatttta
     5281 tgacaatatt taaaattcat atccatcatt gtcatactag tcctcctata ttgaaatgcc
     5341 attaccttga tttattgttc tttagatata gaatattgac gacctttcca ttcaactaca
     5401 ccataacgat gcgtttgttt ccaagattgg atgaaaattt tagtaaaaaa cataaataac
     5461 aatggatgac ataccattaa taaattagaa aattggccga cccttcgatg cagataaata
     5521 aattgtgtag tatagctcaa gtaaagtgct accatttttc ttacagacat ttggcgatat
     5581 tttaaactaa gacatagccc taaaatactc gctatagaac caaacaacca caacacaatt
     5641 gctgtcatga tcttaggctt tgtgccacct gccccagttg acaaatgctt tgtccatcct
     5701 tcttgtaatg actgaaatcc ttcttgatac atgcgaaatg caacaaatgg aaacccttca
     5761 taaactgtta cgggcaatga ttgtgaagta tatgcacttc ctaaagcaaa tccttcaata
     5821 atatgacggt ttgcactttt atgacctcca gttgcataat aatcttcttt atttgttaat
     5881 gtcaccggtc caaatgcatg ctggttagtc cgaccgtcgt ctaaggtaga aaatacattc
     5941 ataccaacga ctgtcattaa attaaatatc gctgaaaacc cttcgtagaa acgctttgtt
     6001 atatgataag gctgtacgct taacaatcct ttttcacctt gtaattgata ctgattaatc
     6061 aacgtttcaa cagcatcttt ccttaagaaa gttacatcag catctacaaa ggcaatgcga
     6121 ttcgtacatg catgcgtcac accttgataa caagcatgtg atttcccata ccatttgccg
     6181 tcagtatcat caacaacatc tactacagtc gcaccatatg aacgtgctac acgtgctgtt
     6241 tcatctgtcg atccgtcatt cataacaatg acatctactg gaacttgctg ttgtattatc
     6301 gaatgtagta aatgacctat tcttttttct tcgtttctag ccggaataat aattgttaat
     6361 gctttatgat tgaagttcag cgttttcgcc tttagctgat gtctacgatt aaatatcaat
     6421 gcaccacacg ccatagacat ggtcactatt actgttaata ttcgtgataa ccatttcata
     6481 gatatcactt cctattcttc gcttctcgcg cgtttatttt gtctgcgact tgttgcccac
     6541 ttaatgtaac cattggcatg ccaccaccag gatttactga tccacctaca aagtacaagt
     6601 tttcaaaata ctgactttct ttaggaaatt taaatccttt gtttttcttt ttatctgcta
     6661 caacaccata tattgcacca cgattagaac gataattttt ttcaatatcc tccggtgtcc
     6721 aaacatcttc ataaataatg tgttttctta aatcagtaag tcccattttt tctaatttat
     6781 ccaaaatttt atccctaaac ttcgcataat cttcagtggt aaaaggctga tcttgaatat
     6841 atggaatatg tggtaagact ttgatatttt cataacctac tggcgcttgt gtgtgatcag
     6901 ttttattcgt atttactaga taaatggtcg gatcatctgg caataccttt tcatgaaaaa
     6961 cttgttgata attgagataa gcattttccg taaaaaagaa attatgatgt gctaattgcg
     7021 ggtattggca agcaacacct aaatgcatca catatcctga gcttgccggc tcaaattccc
     7081 tctctaattt gtttaatcgt tgagtatcaa ggtgaattaa atatttataa gtaggtatga
     7141 cttccatatt tgaaataata taatctgcct ttacaaactc acctgtatct aatctgacac
     7201 ccgtcacacg tctttgatat gttttaatat tgtccacacg tgcacctgta tgaattgtga
     7261 caccttcttc acgcgctagc ttttccaagg cattggctaa atgatggatt ccaccttcta
     7321 cataccaaag gccttgctct tgttgcatat ggaataacat agataataca gctggcgcat
     7381 cgtatgatga agaacctaca tatttgataa aatagcctaa catttgtcgc aagtatggat
     7441 tcgagatacg cttgtctatg gcctgttgca tagtatgtac ataatcataa ttaataagag
     7501 catttaatgg cccatgaaat ttaataattt gagatagtgt atctaaacca tagttgaaat
     7561 accctttttc agtaatacga tcgattcgtc ttgtataatt caaataattt tgcagttcct
     7621 ctatatcctg tttcgacaat atcgcattat gctgacctgt ttctttaata ccttcataca
     7681 aatcgatagt cgttccatct ggaaaaaagc tacgccattg atgtggcaat cgcttgattg
     7741 taacgtagtc tgacatttgc ttcttgctat attcgaataa tttttcaaaa atataaggca
     7801 tcgttaaaat agatggacct aaatcaaagc caaagccatc tgattcatga cgattcactt
     7861 tgcctcctat atgattattt tgttcatata atgagaccga atagccactt tgtgccattc
     7921 gaattgctgc agaaatccca cctaagccac caccaataac gatgatatgt ttagtcatga
     7981 cgttcacctc ttcgttttaa tatgcgtagt aactgcgtta atctcggtcg attatagcgt
     8041 tgcacaataa tgattggaac attagctatc attgcataga aaatatttat atactttact
     8101 aaacgagggc ctttattgaa tatgatgact ggaagtatcg atatccaatg aatcaactct
     8161 gccctctttg tctctataat cattttttca atttcatcag tattgattgt cattaaatga
     8221 cgctgatcat atatattttg attaagctga tgaccatcta aaatcttatg tttccatttt
     8281 cttacgtaaa accatttgtt ccaaaatttt ccatgcttct caaagttaaa tgatttaaat
     8341 attatgtact tctgacgaaa atacttatca ggaattcttg tacctaaatt tgctatattt
     8401 agttgaacaa ttaaccaata catactacaa aaaaatgctg ttttaatata ttttttcatg
     8461 gttttcatct aaattgaatc actctcaatc atactgacat agtataaatt atcactttat
     8521 tacttagttc cctaaagatc agtgaataaa ccgctctcat ttatatttat ataaaacaaa
     8581 aacgccaata ctctcatgtt agagaatatt gacgtcattt gatttacatt tgattaatga
     8641 atgaaattat atgaacctgc ttggtttgct gaaattgtac gagacgtaac cacaccagca
     8701 ccatgtccat agttcatttc tgaaacacga acagaaccgt tgctgttaac gccttcaacg
     8761 taagcaacat gaccgtaata gccttgtgtt gtttgcatga tagcaccaac ttttggtgta
     8821 ttgttcactg tatagccaga tgatgcagct gcgttagccc aattacttgc gttaccccat
     8881 gttgaaccaa ttttcccacc aacacgatca aatacataat aagtacattg tcctgaagta
     8941 tataagttac ttcctgatgc ataaccatta gaaattgaac gaccatttga agatggcgct
     9001 gcagttgtag tcacatgaac attattactt gttgtgctat aacttgcacc tgaaccacca
     9061 gtataatagt tatttgttgc tgtttgacta ttgtttgtat agttattgta ttgatatgaa
     9121 tgattatagt agttattgta agatgcattg ttataactat aagtattgta gttgttgtag
     9181 tagtatgtgt tgttttgcgt taattgactt ggattccaat ttcctgtcca agtgtaatga
     9241 taattacctt gtgcatcaat tgtatacgta tagctgtatg attgagcgtc attagaatta
     9301 tatccattgt tattttgttc tgcggcttgt gcatcatgtc ctgcaaatgc gatagtggca
     9361 agtcctgctg tagcgattgt agctgtaacg atttttttca taattaaata tcctcccaat
     9421 aatcaaaaca ctctatacat atagtcatta gcgactcaat tattttaaat tttatatttt
     9481 ttattttttt agtatgcaaa caattataac ttttatagtt ttgcgacaaa acaaactata
     9541 acagagatta ggtgtcttgc gggctttgtt atcataatgt aatctgttcc taataaagct
     9601 tcattgaatg taatatacca attagaatgt ttacaacact ttttctgcac cgttactatg
     9661 ggatttgaaa ataaaaaagc actaaacttt taatgaaata catcattttt gataataaat
     9721 atcagaagta atgtaatatt tcaaaagttt agtgcatcaa attatttctt atttgtagca
     9781 tgtgtttcca tcgtttttac aatcaaatca gtcagcgctt tactacctgc atattctaag
     9841 tgaataccgt catatgcaaa gtattccgga tgacctgcag aagctttata ccaatcgact
     9901 agatgtacat tagagcgctt ttcagctgcc tcataaatta atttatttat tctaccttca
     9961 taatctctag gtactctaat agaaactaaa tatatgtctg cttttccaaa actatccaat
    10021 agttcattta attgatcttt cgtaaatgcc ccatttgtac caagctctac tacaactttt
    10081 tgaccttttt tagcatagtc tttatattgc gatttcacaa ttggtgtagc atcaacgagt
    10141 tgccgtccaa ctttaccatc aatttgtgca tttggtattt tcttagtaaa gacattacca
    10201 atatccacca tgaccgagtc accaattaat agtggtgatg actttttaat atcctctttg
    10261 ctattcgctg tctgcttatc ctcttgctta tcttttttaa cgactttttt cttcgttgtt
    10321 ttaaattccg tcttcttctc tttcttctcg gcttcatgtt gtttgccaag tgcatcaaac
    10381 tgtccactga gcacaacgat agacggaacc aataatagga taactaacac tgttctagca
    10441 aattgcccct tctttttagg taaaaatgca aaagctttaa atcctttttt acgtataggt
    10501 gtttcaataa agcgatacga aatttcagcc attaacgctg ttaacaaaat ttctataata
    10561 taaacgtata ccggtatttg tccttgtacg taataactgt tcacaaaaac aatgatagga
    10621 taatgccata agtataagct atatgatcgt ttacctataa ttagtaaagg tttcatactt
    10681 aaaaatttag caaataaact agaaggatgt accgcaattg caataatgaa taaagttgca
    10741 aatgatataa tgtaaaatcc tccattatag atccattgat cttggtctcc aactataaag
    10801 aacaaagtca ttagaaccgc aaaaccagat atccctataa tatctaatga tacgacaatc
    10861 tttttagaaa tatctttttt caaagcaaac ggaggccaaa taaatgctaa tatacaacca
    10921 agcaataaag tttgcagtcg tgtatctgtc ccaaaataca cacgtgaatt atctccagtg
    10981 atgaaatgaa tcactatcat aagtcctaaa gaaatcaacg atacaataaa tagcgtttga
    11041 ataatatttc tcggtttaaa tctatgtaat aagaacgtga taaccaatgg gaaaagcaag
    11101 taaaattgtt cttcaatggc taaagaccat aaatgtttga gtggttcaat agcaaattgg
    11161 ttaaaataat ctacattctg tgaaatgtac caccagtttg aaacatagaa tatagctgca
    11221 atagcatctc gtttcatttg tataatcaat tccggtttaa atatcagtgt gaacgtaagc
    11281 acgacacaga ttaaaaataa cactgccgga atgagtcgtt tcaatcgtcg cttccaaaac
    11341 tctagcaaat cgattttttg cgtccgataa tactcactta tcaacaaact tgttattaaa
    11401 taacctgaaa taacgaagaa tgtatctact cctaaaaagc ccccacttaa ccattgtgca
    11461 ttcaagtgat aaataatgat tcctataact gcgaatgccc tcaatccatc taatccaggt
    11521 aagtatcgcg gggaatacat tttttctaaa cgtttaaagt cttttgtatc catgttaata
    11581 aacgccccat ttatttttct ctattttgta gcatatcaca atatttttga aaataaaata
    11641 ttgcactgat tttcattaat tgatttaacc cttaattaag atagttttaa attttttatt
    11701 aagtagaaaa caattattac agttgatttc attactgcaa accacatata aatttgtcga
    11761 ttttactaca taacatagat tatcatagat tcttgaattt ttagcaaaat aactgttatt
    11821 ttcattatat ttttacaaaa aaggttcgtt ttatatttta tgcatattac tgtaacagaa
    11881 tcattaagat atgctattcg aatatacttt ttcaaaattt atataatgaa taaattaaca
    11941 tgtattgaaa aaaaagcgaa atgcagccta tcctctaatg taaaccaaac gatatatctc
    12001 gtcagacttt atatttaaac gctatgtgtc acttttaaaa tgaatattac taagattgtc
    12061 atatcaatta ttattgcatc gaattaatct tttaaatttc tgtaataaaa cctattatct
    12121 acatctaatt taggaaaact acttggtaaa tctcctcgct taatttcacg gaagccatta
    12181 ttagaataaa aatactgtgc actaataaat ttatcaattg ttcctaaata aataccatcg
    12241 atattttgtt ctttacatgt cattataact ttatctaaaa gttttttacc tatttttaaa
    12301 ttcctataac ctttatcaac aaacattttt taagtgcaga catattatta tctagtctaa
    12361 tcaaacctat agtaccaaca atattttgat gattgtttat tgcaagccaa aattgccctc
    12421 cattattcaa atagttatgt tcgatgttct ccaaatcagg ttgatcatct ctatcaattt
    12481 ttatattaaa ttcatttttt tgaatcgata aaataaactc gattagctct tccttataag
    12541 acctattata ttcaattatg tttatagcca tttttatctc ctttttcatt taatttaatt
    12601 ataaaatgtg cgtttagttt gtatctagtg tactcagtac agcctcaaat gaagtttcat
    12661 tccacttggc acttaataaa gacaagtatt ttagcagtaa tacaataaag tccaataaat
    12721 ttccctaact tcaatatcca ctttttaaaa aatgtatttt taattaataa aaaaactctc
    12781 cccaatttct atgggaagag ctatatattt aatgtctaaa cattactttt atttattatg
    12841 aaggaattag aatccccaag cacctaaacc ttgtgctttg tatgctttaa cagctgcgtt
    12901 gatttgttgg tcaacagtgt ttgttggacc ccaacctggc atagtttgga ataaacctga
    12961 agcacctgat gggttgtaag catttacttg accatttgat tcacgagcga tgattgcagc
    13021 ccatgtagaa gctgaaacac cagtacgttg agccatgatt tgagctgctg atgaaccagt
    13081 agcacctgca gtattaccat tgcttaatct cactgaactt gaagtagttg aagtgctgta
    13141 gttatggtaa gttggagctg aaacagcttc aacgtttgag ttacttgatt gtgcattgta
    13201 gcttactgat tgtacatttg aaccttggtt gtatgaagta gtgtagtctg cacctgcaac
    13261 gtttgagaaa ccagcagttt gaccattagc tgcttcatag ctccatgacc atgtagtacc
    13321 atttgaagtg aagttatatt ggaaaccatc ttttacaaag tggatgtcat atgcaccatc
    13381 tttgattgga gctgcattta attgatcttg gtgattatgc gctaagtcaa ctaagtgtgc
    13441 ttgatcaacg tttacttcag cagcgtgtgc ttgatgtcct gtacctgctg cgtaacctgt
    13501 tacacctaat gccactgcta atgatgatgc cataattgtc tttttcatag taaaaaatcc
    13561 tccagtaata attgtaagtt tatgttttta gtaattatat tttgaatttg aatgtcgtag
    13621 tgcaagttta aattgtcttt tatttctttc aacggtactc actatatcac aaaaaaccag
    13681 ccagtaaatt acactttctt tacaaaacat tacaatatca agtgttattt gtaatgttga
    13741 aatatggctg ttttatactg taatgtgaaa tatgtgccct ttagaatcca atcaaccctt
    13801 gaaatagtct ttaacacata agatttttac tatatttagc tcaactatta cagctttcgt
    13861 aatattacag attgtatttt tgttacatag ctgtaatata tctgacatgt aacttctctt
    13921 attttcgtac attaaacgca attaaaaagc aatcaacaaa tatgtttcta cacatgtatt
    13981 gattgctatt attgttgtat attcaaagtt ttaaaacaca catcttttgt gaattgtctt
    14041 atcttttatt agcgcaaata aactgcagct caattatatt gttcaacttc attctcgcaa
    14101 ttcacaataa cattaaataa tttttggtct catattttca aaaaacatac tgttattatc
    14161 ccatgaattt aaaaatatca ttagtatata aacgaaacac tttacgataa atgatatctg
    14221 caagccaagc tgttacaaat ggtacaacaa agaacgctac tacaattagt aagacactca
    14281 accaagcaga atcaacctcc ataaatttaa atgcattaat cggtcctacc attcctataa
    14341 aaccaaatcc agctgactct ttcgttccat gaatacctac taatgctgat accaaacctg
    14401 atacaatggc tgtcgttaat attggtaaca taagaattgg atatttcacc atattaggta
    14461 tcatcatttt aacgcctcca aagaagacgg ataacggcac ccctaaacga ttcactttac
    14521 ttgtaccaat tatcaatact gcttcagtcg cggagatacc aattgacgct gatccagctg
    14581 ctaaacctgt aatacctatc gcaaaggcaa tggccacagt tgatagtggc gaaataataa
    14641 taagactaaa taccattgaa atcaaaatac tcatgacaat cggttgtaat tctgtaaaac
    14701 cattaaccat attaccgatg gctgttgtaa tcattttcgt atacggcaat attaaaacac
    14761 caattgcacc tgaaataccg ccaacaactg ttggtaatac aatcaatgcc atactaccta
    14821 cgcgatgttg aataagtaaa atgaataaca ctgcaatcgc tgctgtaatc attgtattaa
    14881 ttaaatcacc aatacccgta atcatccaag caccattttt aaactgcgct gcaccgcttc
    14941 ctacatatgc tgcacttgcc acaacagcaa ttgctaatgg cgataggtca aatttcatgg
    15001 caaccaatgc accaatcaaa gcaggtactg taaattgaat tgcaacgaca acgcctaata
    15061 acgttttaaa aatcggatga taatccataa agtatttaaa aatttctcca agtatcgcat
    15121 taggaactaa acccgcaaca atacctatgg cgacacctga taaaactcta aatataaaat
    15181 ctttgggtgt aattgtttta attgatgtca taatatcatc cttccattta tgtatataca
    15241 tctgtatgca aataataaag agccttaagt tataagctgc cactagctta aattctaaga
    15301 tgtgcatgcc gatgttgtta tatttaggct agcagtatca tctataactc aagactatga
    15361 aaaatagtat atcacaaaat tctgaatttt cagataaaga aattggcaat ttttcaaaca
    15421 tattgttaca atacactttt attttatctt catttttaaa atccattaat acaatagaag
    15481 aaagacattc aaatgcttac caaaaaggta cattatttgt taggagcgta tcagcactta
    15541 catatcatca acacaattga caatataata gaagatactg ataataagtg ttaaaacaac
    15601 agatgttagg tagtgaacaa atgatggaaa gtaaatccat agatccaaga atcgttagaa
    15661 ccaaacaatt gcttgtcgat gcttttctta aaatttctag agaaaagaaa ttatctcaaa
    15721 ttacagttaa agatatcact gatattgcta cgctaaatcg tgcaacattt tacgctcatt
    15781 tcactgataa agaagacctc ctagactaca cattatctgt aaccatttta aaagacttga
    15841 atgataattt gagcatttct aatgtcatta atgaaaaggt tctgcgtaat attttcattt
    15901 caattgcgag ttatatcaaa gatgctgcaa agtcttgcga attaaatagt gaagcatttt
    15961 gcaacaaagc acatcaacgt attaataatg aattagaaga tatttttgcg attatgttag
    16021 aaaacagcta tccggagcat caacgagata tcattgtaaa tagtgcgagt tttttagcag
    16081 ctggtatctc aggcttagca ttacattggt ttaacacgag tcaagagaca gccgatgtgt
    16141 ttatcgatcg caaccttcca tttttaattc atcatatagc acatttttaa taaaacttgg
    16201 tatttagtca tgcatcttga aatcactatg tgacttaggt tcatacttgt acacacaata
    16261 aaatttaacg tattacgatt gattagccgt gtctaggaca taaatcaacg tcctatactc
    16321 tacaatgtca tattagcagt cgttaactga atgaaaataa gcttgtcatt aaaacatata
    16381 gattttagtg acaagcattt ttgtttttgc gtacttaaac aacacttcag gcaatatgtt
    16441 gtttaggcaa caaatgatat gtgcgtgttt attggcaaac gtacgacata gtagtatagt
    16501 atgtctaaac aacatatgtt gcatagttga tatgcgttgt ttaaatacta agataggagg
    16561 gattgacgtg agcgagacag atgaacctca ggggtttgaa cgcacgcata atatattaaa
    16621 tattaatcag agtagtctgg gtgtagtgac atacattaca aataaattaa agtcgacgtt
    16681 gaagcaacac ataataattg ctcgtggtaa aaagcgaatc gactatcgac tgtcgtataa
    16741 cttttacact acgtattatg ataatgtaga aatcaagaaa atcgactgtg aatataccta
    16801 tgctatgccc attgcaattt taataagaca cacgatgtca ttcgacaatg ctcatttctt
    16861 tgctcagtta cgtcatcctg tcttataaaa caacattgca gacatgtata tcaaacgaca
    16921 cttcaataac atcactttgc catcgtacta ctagtaaaat cgtgtctcaa atcccttatt
    16981 ttaattccaa aaatctgctg gtcaaaagac cgagaaacta aaaacattac ttaatgtgtt
    17041 gataaattac catataaaaa taatctcaaa atatatcaac acttgattct aaggaggata
    17101 tgacaatatg aaaattttag atagaattaa tgaacttgca aataaagaaa aagtacaacc
    17161 acttactgta gctgaaaaac aagaacaaca tgcattgcgt caagactact taagcatgat
    17221 ccgaggacaa gtattaacaa cattttccac aataaaagtg gttgatccaa tcggtcagga
    17281 tgtcacacca gataaagttt atgatcttcg ccaacaatac ggttatattc aaaattaata
    17341 tttgctcacg aggtattgca cttaaggtgc caactgacct cataaacaaa gcccatactg
    17401 attgaagaca ctaatgtgtc gcaccatggt gcacattacg cttcatctct gtatgggctt
    17461 tttatttatt cttttgagaa tttcatttta gcagaccaaa aaattaaaat ggtgtccatc
    17521 aagatctttg aacattgctc cataaaaccc ttggcttact gttggttctt gaacgactgt
    17581 gccacccgct tctttcactt tattcaccaa ctggtcaact tcttcatttg tgttcacacc
    17641 tagagaaatc atcacttcat ttggttcagt attaaggctt tgctgactta cattttgaaa
    17701 atgcttgttt tctattaaaa ttacggttgt ttgacctatt tgaatgccga ccattttatc
    17761 taacatttgt gggtttctat ttattttaaa tcctaacgct ttataaaact gtgcgctctt
    17821 ttctaaatct tgcacatgca aattaaacca cattgattga atcatgattg caccccattc
    17881 attacttatt atagttttgg actttaagcc aatcacttaa tgataatctt gttggattta
    17941 tttcagccat taattcaaag tctacttcat aacctttttc ttccaaccat tgcttttctg
    18001 caacaccact aacaaattct ccttctataa cagtagattt acctgtcact tcactaaaaa
    18061 ttgttgctgc ttcacttaat gtaacttcat cggaaccaat ctctattgat tgatgcgtaa
    18121 agctttgtgg atgtgcaaaa atatacgatg caattttagc tatatcaata gaagaaatca
    18181 ttgtgaattt tatattcgga ttaataaatt ctggtaatgt aatacgttca tcttcgactt
    18241 tagcaatgcg taaaaaatta tccataaaga atgatggttt gataactgtt gcatttatat
    18301 tagattccat taatctattt tctatttttg ctagtacttc aaagtgtggg ccagttcgat
    18361 ttcgattaac ccctcccgca gtactataca caatatgttg aatattttct tgctcagcta
    18421 tttcaattat cttcatacct tgtcttaatt cttcgctaac atcatcttta acgattggct
    18481 gaatactgta taagccatac ttacctttca tcgctgattg caaactaaca ttatcactca
    18541 gatcaccttc aacgattgat aaatgcggat gtcctatgtc tgaaagttta cgattattct
    18601 tatttctagt taatgcactt acataccatc catcctctaa caactgtttt acaactgcat
    18661 taccttgctt ccctgttgcg cctattacta aaatatcttt cattttagat ccactcctct
    18721 ttttatctta aaaaccaata gtcctctttt tcacctttta aatacgtata tggttctggt
    18781 gtttgtccat ttcctttaat attgtcgttt attacatctc tgacatccca tactaaatga
    18841 ataaatcgtt gtaattgatg actaaaaatg cctagtggcg ctgtcatagc taatactttt
    18901 tccacatctt ctgtagtgac attcagtttt tcagcaatca tttgaaccga taaggtagat
    18961 gcttcgaaat gtgcttgcaa ctcttcagct gtgatgcgtc tttgctcttt atttaaagac
    19021 attatcaaca ctcttttctt ttatgatatt taatagttat tgcaaattca ttgctaactt
    19081 tcttaacacg ttttataata aacggagagt tatccgtttg tcaattttag agaattttaa
    19141 gtaggtgtaa tcatgcgaaa agatgcaaaa gagaataggc aacgaattga agaaatagcg
    19201 cacaaacttt ttgatgaaga aggcgttgaa aacattagta tgaatcgtat tgctaaagaa
    19261 ctaggtatcg gtatggggac attgtatcga catttcaaag ataaaagcga tttatgctac
    19321 tacgtcatac aaagagacct tgatattttt atcactcatt ttaagcaaat taaagatgac
    19381 tatcattcta attatgaggt tatgcaagtg tcgttagatt atcttttaca atttaaaatc
    19441 gataataagg cactactgca atgtattgaa gcaggcaaca ataaattacg tttttaccaa
    19501 agcgcatttt atcaagaatt atttgatttt tattatgact tatttaaaag cgatgacgac
    19561 acttacacga agttcaaaac agatatgctg ttgcaatcat tatcaactag tgtatttgcc
    19621 ttccaaatag agcatcgcca tatttcaatt gaagcgtaca gaaattattt attaaacatt
    19681 tatttagatg aggtgggacg taatgactaa aattgtgtta atcacaggag gcaataaagg
    19741 gttaggctat gcaagtgcag aggcacttaa agcattgggt tacaaagttt atataggttc
    19801 tcggaatgat gtaagaggac aacaagcatc acaaaaatta ggtgttcatt atgtacaatt
    19861 agatgttaca agtgactact cagtcaaaaa tgcttataac atgattgctg aaaaagaagg
    19921 ccgtcttgat atcctcatta acaatgcagg tatatctggt caattttcag caccttccaa
    19981 attgacacct cgtgacgttg aagaagtata tcaaacgaat gtattcggta tcgtacgaat
    20041 gatgaataca ttcgtccctc tcttagaaaa atctgaacaa cctgttgtcg tcaacgtatc
    20101 aagtggttta ggttcatttg gaatggttac aaaccccgaa acagctgaat ctaaagtgaa
    20161 ttcattagct tattgttcgt ctaaatcggc agtaacaatg ctaacgctac aatacgctaa
    20221 aggattacct aacatgcaaa tcaatgctgc tgatccaggc gctacaaata ctgatttagt
    20281 tggtgatttt agtaataatt ccaaacacgt ttctgaagga atcaagccaa tcattcaatt
    20341 agcaacgatt ggtgcagatg gcccaacagg tacatttatt aatggcgatg gagagatgcc
    20401 ttggtagatc aattttatgt tacaaacatt gaaattagag gtatataaaa aatgcttctg
    20461 caatagatgc aataaacatg caatacaata tggaggcgaa gtaaatgaaa agtattacgt
    20521 ttgaagaaca ttatgtcatt gaagatattc aaaaagaaac gatgaatgcg atatcagcag
    20581 atcctaaagg tgtaccgatg aaagtaatgt tagaaggcct tgaaaaaaag acaggtttta
    20641 caaatgccga cgaattatca catcatgatg aacgtatcca atttatgaat aatcaagacg
    20701 ttcaaattca agtcttatct tatggaaatg gttctccttc aaatttggtt ggtcagaaag
    20761 ccattgaatt atgtcaaaaa gcaaatgatc aattggcaaa ctatattgca caatatccca
    20821 atcgctttgt aggctttgca actttaccta tcaatgagcc tgaagccgca gcgcgagaat
    20881 tcgaacgttg catcaatgat ttaggattca aaggtgcgct cattatggga cgtgcacaag
    20941 atggttttct tgatcaagac aaatatgaca ttattttcaa aacagctgaa aatttagacg
    21001 taccgattta tctacatccc gcgccagtta acagtgacat ttatcaatca tactataaag
    21061 gaaattatcc tgaagtaact gcggcaacat ttgcttgttt tggttatggt tggcacattg
    21121 atgtcggcat tcatgcaata catctagtat tatctggtat ttttgatcgt tatccaaagt
    21181 taaatatgat tattggacat tggggtgagt ttatcccatt cttcttagaa cgtatggatg
    21241 aagctttatt cgctgaacat ttgaaccacc ctgtaagcta ttactttaaa aataattttt
    21301 atatcacacc gagtggcatg ttaacgaagc cacagtttga tttagtcaag aaagaagtag
    21361 gtattgatag aattctttat gctgctgatt atccatatat tgagcctgaa aaattaggtg
    21421 tatttttaga tgaactgggt ttaacagatg aagaaaaaga gaaaataagt tatacaaatg
    21481 gtgctaaatt attaggttta tcacctaaca attaacaaat ggaggtcatt gaatatggaa
    21541 actttagaat tacaaggcgc taaattacga taccaccaag tcggacaagg acccgtgctc
    21601 atctttattc ctggtgcaaa cggtaccgga gacatttttc tgcctcttgc agaacagtta
    21661 aaagaccatt ttactgttgt agccgttgat cgtcgtgatt atggagaaag cgagttaact
    21721 gaaccactcc ctgattccgc ttcaaaccct gacagtgatt atcgtgtcaa acgcgacgca
    21781 caggacattg ccgaacttgc aaagtcatta agtgatgaac ctgtctatat attaggttca
    21841 agttcaggtt caatcgttgc gatgcatgtg ttaaaagatt accctgaagt cgttaaaaag
    21901 attgcatttc acgaaccacc aattaataca tttttaccag acagtactta ttggaaagat
    21961 aaaaatgatg acattgtgca tcaaatttta acagaaggct tagaaaaagg catgaaaaca
    22021 tttggcgaaa cattaaatat cgcaccaatt gatgcaaaaa tgatgtctca acctgctgat
    22081 actgaagaag gacgtataga acagtacaaa cgtacgatgt tctggttaga atttgaaatt
    22141 agacaatata cgcattcaaa tattactttg gatgatttca caaaatacag cgataaaatc
    22201 accctactaa atgggacaga ttctagaggc tccttcccac aagatgttaa cttttatata
    22261 aataaagaaa ctggcatacc tattgtagat attccaggcg gtcacttagg ttatattcaa
    22321 aaacctgaag gctttgctga tgtattatta aatatgtggg gttaattgat tgttctctaa
    22381 taaaacagct tggacagttt tcaaattata tttgatggct gaccaagctt atttttattt
    22441 acgcttaaac gttcaaatca actcgttatt atacatgaag gcatgttaac cattctcttc
    22501 cactgaaaac actactgttc caaacattga tagtcttctt ttgaaatacc ctttccgata
    22561 agcaccaaat attctggcac tttttttgaa aatgctacag gtgttaattc atattgtcct
    22621 tgtgtaaatt gaatgagata cgtatgtgcg gtgtcttcaa atgtcataaa ccctttcaac
    22681 ctataaatat gagacggaag gcatgctaaa cgttttataa actctgcttt agtacatttg
    22741 actggtgatt gcacaaattg atgattaatc atttgatgta aagacttatg tttattgtca
    22801 gaagttgctg tcatttgtct aactgatatt ggcaaagtga cgctgccatg cataccgact
    22861 tgtatatcgg cctctgggtt aataacttct aaatctttca atagtttgct cgttgtttca
    22921 acatctgctg aatctatttt attaacaaat aagacagaac aatatgctaa ttgctcataa
    22981 aataagcctt ggatgtcttt agggaatgat ttaatgtgtt tatacatgct tgcgtctatt
    23041 acaccaatca tatgtgtaat tgttgtaaac ggagctaaaa taggcgttaa acaagcatct
    23101 aagacagaga ccggttctgc aatcccacta cattcaataa atacaatgtc tggttgctct
    23161 tttaaatata attgatgtaa ctgttctgat acatctgctt tcattgcaca acagatacac
    23221 ccctctgtca gttcacttaa aggtatgtct tcagatacta aggcaccatc aacactcatt
    23281 ttcccaaatt cattcatgat gactgctggt ttcagcgatt cctttaatga ttcagcgagc
    23341 aaatgattta agacagtcgt tttaccgcca cctaaaaacc cacctataat aactattttc
    23401 atctgcgact ctcctcttat acttcatatg aatagcatat tccaagatag atctcctatt
    23461 gactgacatg actctatatt cagttatagt tcattttttt taaataatca gcattactat
    23521 tttaacaatg atgcctacac caataactaa agacaatgat gtcaccattt gctttccaat
    23581 taattttagt gctgactgac cacctaaatg tttcaaaagc atcgtcattg tgaccgagca
    23641 cgctgtaaat gttgaactaa aaaacacaag tagtagtaac tgtgttgctg tcattccttg
    23701 taataaggcg ccctgatgca aattaaacaa gagcatgccg tcttttcgaa tcattgaaaa
    23761 taaaatccct ggtgacaatt ctgacgagat gcctaataac gataatatag gtgtaaatat
    23821 ttgtgataaa acattcaaaa ttggcgttag tgataaaata ctaacaataa gacagattgt
    23881 gataaaaata ggtagcgctt gaacgataaa catttgtata ttttgccaca tttgtagcaa
    23941 catttgacgt atatttggca tatgtaattg cctatcataa ggtagcggaa cgctaagttg
    24001 ttgatcattc tttttcaacc atatcctatt atgtaagatg ccacctaaaa gtactaatat
    24061 taagtacggc ataaatagcc atgactttcc agctacacta aaaatagata atgtcgcacc
    24121 tatttgataa ctacaagaac taccaaagct tattaaactc atacactgtg tcttcgtgca
    24181 ggtatggcat tggtgtgctg cttgtgaaat agctgctgca ttacatccaa acccttctaa
    24241 tagtggcacg atatcattac cttgtaatcc tatccataac attgacggtt caattgccca
    24301 tatcatccat gatttgagtc ctgtttgatc aattatagca gttgataaac taatcaatat
    24361 tacaaccggc aatgcccata cgagcgaata tgttccaagt gatagcacac cataatcacc
    24421 aaataatata tgctgtaata tatcgtgatt gagcaatgtt gtttgagtta aatattctat
    24481 ccatgctgac acatactgat tttgaaacca atctgaaaat tgataagcga taccgattgg
    24541 caatgcaaac attaaaaata tcaacaacca ggctaaaaat gtcgcactta ttttaggtct
    24601 taaatgtcgt cgctcatgtt gaaatagtgt caattgttct atcacatgtt cactcgctat
    24661 aggcgaagaa actacacttt ctgccaatga aatatctaat ttctgtttta tgattaataa
    24721 tcttaatttc gctttttctg ttgcggtaag tttatccgca tgactaataa ccaaataatg
    24781 aggttcatta ggtatgggcg tataatgccc agttctcata tcatatttca cttctaacat
    24841 gtgcatgaca cttcacaaca tgaataatca tctaaataca tttgattttt ttgataattt
    24901 tcaatgacat cttgtttagc tggtaagcct tcccgctgtg ttaaaagatg tgcaagtact
    24961 gcaaatcgcg ctctaaattt atagatatag cataatttgg cattatcatt ttcaactgtt
    25021 gcaccaatca taaaaatatt cggataacgt gtcgattcat catgtgttgt taatttaata
    25081 tcttgatttg ttgtcacaaa taattgttga acgattggat tttttgttgc atcaaagcca
    25141 gttgctagta ttggttcatg aggtgtaagc acactttgtc cgctatcaaa actgatatga
    25201 tactgtccat tgttaaaatc aatatcttta actgtataat gtacattcat ttcgatgcga
    25261 gcaccttgct taatgacatt acctagtcgc tgacgtgtat aaggtgacaa tctaacacta
    25321 ggatcagcat ccggatcatt taaaccggtt gtgctagtat aaagtgcgat gtcagagcca
    25381 ttttttgcaa gttgatatgc agcatcaaag ccactttcat tacctccgat aaccacatat
    25441 tgccccttat taaagttatc aaagtcttca atttcactat aatgaatacc atatttaaat
    25501 ggctttttag ggaaattata atcacctgtt gcgacaaaga tataatccgc gtgatatatc
    25561 tctgttgtcg ttgcaatcgt ataatatgca tcatctacag atatatttgt gacaactgta
    25621 ttttcaaaga tattcagctc gtaatggttg gcaaccactt gtaaatattc agcatatgtt
    25681 tctccggaaa tatgttcttc attaaatgta aatgctggtg aagtatccat ggaaattgca
    25741 ttcatatcag gcatgccaaa tccattagac gtaaatgatg gcgtgatcgt acgggtcgat
    25801 ttcggccaat gtttaaatga atgtcctact gttccttttt ctaaaataat gacatctgtt
    25861 ataccgaaat cttttaaggt aatggccata cctatacctg cagcaccggc accgataata
    25921 gccactttat gatgttgcat attaacactc cctcaatgat ctatttcaag ttattgtttt
    25981 tgattataaa tcgtaataat tacgatttat aaagtataga cctatccttg agaaatttca
    26041 atattaaaag aaaaacttcc gttattgtag agaattcatc tacatttcgg aagttggtat
    26101 gtgtcttttt ttaaaattta aggctgctac gtttagttca aatgattatg aatgacattt
    26161 catggtatca tccagcctca gtacttggag ataacatttt ggcggtgaga cagaaatcat
    26221 atttttacaa aatttatttc gtcgtcccac cccaataaag aaaaagtatt tctttattgt
    26281 tgtgtaactt ctccacgttg tatttcacta tccaaatttt gtttcatatt ttcaaacaac
    26341 tcttgaaatc taacctcatc ttgatcagaa taacctttaa aaataactga atctatattt
    26401 tcctcgttaa aataataaat ttcatcttct acaacaccaa tcggatattt acatgcagaa
    26461 tagtcaaaca tatacttttg attttcaatt tctacaattg gtcctctatt aataatcatt
    26521 aacttttgcg aaccttcttt taaataaata atgcttccta ttgtttccat ttattttcct
    26581 cctttgtcac cgtctcaatt tcaacattcc ttcgcatttc caagtcaatc atctcttctt
    26641 ccactttacg aatgccatgt tgaatttgct gttgctgctc gtataacgct tccgttgaac
    26701 gtcgtttcgc aaaatgaaat tcatcgcgga actgttccat acgactagcc atattgctac
    26761 tataataacg ctcacctaaa tgttgatata attgatctat cgaatgatta agcgcatcac
    26821 tttcatctat caatgcttta acctctcgct tttcatcata taagtcatcc atttgctcat
    26881 gtttcgctgt gattatttta ttgatttcat ccagtttatt cgacatcatt tcaccacgtt
    26941 ttaattttta ccaatttcat taccaatcat tacgtcagtt tggtcaaaag cctcaccaac
    27001 tttttgtata ttatcgctcg cagttcgtat tgcttcagca acagattgat tcttactcat
    27061 caaatcatct atcacactat gagcattatt atttccactt actgttgtac tttcatcttt
    27121 atccggtttc gacacattat tacatgtttc aacagcgctt ttaatagatg atgatgcact
    27181 attggtctta aaaatatcac tttgaactgt actcatcatt tacacctctc tccatttaaa
    27241 ttattttaaa attattagaa actgtatcaa tatatgatat tcaaacattc aattatactt
    27301 aaatattgtt ttatgattat attcaaaatt attgtatcaa taaatatgta tcattcctag
    27361 aattttattg aacaaaaagt tataaaatca cttttattgt aactcaacct tgttttacat
    27421 tacagaaatt aacaatagcg attaaaattt tattttccaa caataaaaac gcacaaatat
    27481 ttttattgag gcagctgtat cgcttctcat tgtaaaaata tctgtgcata ttatctattt
    27541 atctcaatct aacgtcttaa ctaacttgtt gtatcttgta aaatcttatc catagaatgc
    27601 gcaacactat cacggtacat ctcaccaaat ttcaataaat ggaccatcaa taaatataaa
    27661 cgataaaatt caagtctata ggatgcacct tttgcgagtg gataatgttt attatacgca
    27721 tcataaaatt cgctcgtaaa accaccaaat accgttgtaa taccgatatc gaattctctg
    27781 tcaccatata atggcgctgg atcaaataac gccggacgac catcttgtaa gaacatataa
    27841 ttaccacccc ataggtcacc atgtaataaa gacggtttac tttgatgctt ttctaattcc
    27901 gccacaattt gacgtcgcac tttgtcatat actttgatat cgttggcatc ccatagccct
    27961 ctatttagca actcatcttt caaatggtcc aagcgtttgt cgacaaaaat ggtacaccaa
    28021 tcgtcttgcc aatgattatc aaaagaaata tcgccacctt cataaggtaa tgagaagcca
    28081 aatttgcctt cttcttgttg ctgactgtgt aattgagcta cgagttgccc taattggcgt
    28141 tgactccctg aagccccttc ttctaaatac gtcatcacta aatacgcatc accgttaacc
    28201 tcgccacttg caattactct aggtgccgtg atacctgcac gttcaaattc atttaaacct
    28261 gcaatttctg cagcataaaa tgattcttta cgtccacgtt ggacaagtaa gaaaaatgta
    28321 tccgtatctg tttcgactcg atatgcttcg tttacatcac caccactcac tggtgaaatc
    28381 tctttaatat cttttaaagg taaatgctct aaccattgtt cattcataca aacaccttcc
    28441 aatctactta ctttatacta taatattgcc taattaggat gatttaatct taaaaacgtt
    28501 attcaaactt gcgtcaattt tcaaaacttt ttagttttaa ctaggaaatt atgtatggaa
    28561 atgctagtct attaaaagaa tatatttcac atatattaag ttgaggatag taagcctcaa
    28621 atataatgaa ataggagcat actcatgtac aaattaatta aacctttctt attcaaaatc
    28681 gaacccgaaa aagcacacgg actaactatc gatgcattaa aaacgttaca aaagtttccg
    28741 gttttattcc cagtcgtcga taaactattt acttataaga atccaacgtt atcacaaacg
    28801 atacaaggta atacgtacga caatccaatt ggcttagcag ctggtttcga caaatcttgc
    28861 gaagtaccaa aagcattgga acaccttgga ttcggtgctt tagaattagg tggtatcaca
    28921 cctaaacctc aaccgggtaa ccctcaacca cgcatgttta gattattaga agatgacgcc
    28981 ttgataaatc gaatgggctt caataatatt ggtatgaaca aagcactaag tcatttgcgt
    29041 aaaaatgctt atcaagtacc tgttggtatc aatgttggtg tgaataaaat gacaccttat
    29101 gaagcgcgtt atcaagatta tataaaggtt attgatacgt ttaaacacga cgtttcattt
    29161 ttcacagtca acatcagttc tccaaatact gaaaatcttc aaaacttcca tgataaagat
    29221 gaattttcaa tgttatgcca agctttaaca gcatttaaaa aacaacatga tgtaacagtg
    29281 ccaatttatt taaaactaac gtctgatatg gatttcgatg gcttaaaagc actattacca
    29341 gcgattactg agacatttga cggtatcatc ttagcaaaca caacgcgaca acgagatggt
    29401 ttaacttctg ctaataaagt cgaagaaggc ggtttgagtg gtcgtccatt atttgaacgt
    29461 aatttaaaat tgattaagta tgcttatcag caaacaaatg gtgaattttt aattataggt
    29521 acaggcggcg tattcagtac tgaagatgca atcaaaatga tgcgtcacgg tgcgtcactt
    29581 attcaaattt attcatcact tgttattgaa ggcccaggtt taactaagaa aatgaacaaa
    29641 ggcatcgcac gttacttaaa agatcatcat tttgacaatg tcagtgatat tataggacta
    29701 gatgcctaat ccaacatagt caaataatac tttaaaactt gctgaatcgt cttattaaaa
    29761 ttatgatata actcaagaag atatatttat caccattttt caaaataatg gcttaaatat
    29821 cacatatcat taaatatagc gttttataac ggaggcgttt gttatgacta aagaaacaga
    29881 taataaaaaa acagtagcga cttctgacaa caaagacaca cagcaacaaa atcaacctga
    29941 agaagatgaa cgtcaaggtt tgaatggtta tcgtaaaaca gatcttgatt tagaaatcga
    30001 gcaagagcta cgtgaaatga tgaaaacagg agaaaatgaa acgaaaaatg actataaaaa
    30061 gtttaaagtg ttttcactta tttcaacact tgtcattgtc attttagcaa ttataagatt
    30121 tgttcataaa atgatgtaat tagagtgaga cattgtttta tgtctcagga tccagttatt
    30181 cattatatct acaatattta cgattatata aataacccga gattttagta tgattcattt
    30241 cactaaaatc tcgggtttct atttgataat ttttaatggg atatggcatg tatacgttct
    30301 tgccttttat ctcatttcca atgattaatc tggatattgt tctaaaaatg ctttcgcttc
    30361 tttattaact gttttaaaat caataccttg ttgcatcgct gcaaagacac atccacaata
    30421 acactgccta aagatattat agtcattaca catttctatg gaacgctcat aacctttact
    30481 tttcttaaaa tcacttggca aatagttcac atcgtatatt ttttggacat ccataccaag
    30541 ttcattgatt aattgtgcgt tctttttagg tgataatgtg attgcactgc caaaataatc
    30601 atagccatgt tctacagctg ctttcgctac aatatccaaa cgcatttcaa aacaagcggt
    30661 acaacgtaag ccgccttctt tttcatcagc taattcttta tccttcacca ttttcacaaa
    30721 cttatgcggt tcataaggtg cttcaatata cttcacattc gcaccagtct tgcgattaaa
    30781 atcttccaca aattgttctt gcactttagc acgtcgtaag tactcatttt tcggatgaat
    30841 atttgaattc gcgaaataaa ttgcaatgtc tgcatattgt gttaaaaact ctaatgtata
    30901 tgtactacaa ggtgcacaac aactatgcaa taagatttta ggtctgattg cttctctttc
    30961 ccactgcccg attaatttct tcaacacctt gtcataatta attttttgat ttttcatttt
    31021 actgataata ggttcagcat taatcatgat ttaacctccc acatttaatc attaacttct
    31081 attatatatg attcatatta aatgtcagtc aaaaaagtta gaaattcatt ttaatgcatt
    31141 tatatttcga aaatccctta tgtatctaaa agcatttttt aaacttgaat tttaaaacac
    31201 taaacaacac atacgtctct gtgtcatttt cattttttgt atgtcatata tatgtttact
    31261 tcatttaaat caatttcatc ttataattta tcgtgtattt tacaaaagat tgacttcaat
    31321 tcatcgtaaa agttatactt ttgccatttt ttaatgtaac atggtgttag taataaaaat
    31381 aatacattga ggtgttttac atgacagcat tattccctta tatcgctttt gaaaattcaa
    31441 aagaagccct tgcatattac gaagaagtat ttggtgcaac tgacgttaaa cgtttagaag
    31501 ttggcgaaga acaagcgtca cattttggta tgactaagga agaagcgcaa gaagcaacta
    31561 tgcatgctga atttgaagtg cttggcgtaa aagtgttatg ttctgattct tttggtcgcg
    31621 ctgacaaaat taataatggc atatcattat taattgatta tgatgttaac aataaggaag
    31681 atgctgataa agttgaagca ttctatgagc aaattaaaga tcattcttca attgaaatag
    31741 aattaccgtt tgctgaccaa ttctggggtg gcaaaatggg cgtctttacc gataaatacg
    31801 gtgttcgttg gatgttacat ggtcaagact atactgcaat ccaacaataa tgttattgat
    31861 aaagatatca tttaataagc ttatgttatt tagcaaggca agccactctc gattcatggc
    31921 ttgccttatt ttattgtata tttttaatta tcgttgctgg cccttgattg acaatcgtat
    31981 taaaatgcct tattaagtca actttgtcta tacggtttgg atcttctacc caatgtctta
    32041 taaaagacaa tcccgcacct gaaacataac tcatgaaata agaaaatggt ataccattaa
    32101 tttgatcatt ttcattttta taacgttgta aatgacacat aattaaatca taaagttttt
    32161 cgtggatatt tgaatcttta cccatacgaa acatcaattg ataaaatgcg atgtcttttt
    32221 ctatcatttc tattaaaacg gtcataattt gatgtatgtt atccgtggat aacttaactg
    32281 ctccatttaa cttctcatca tgaatgaagt ctcttatttc ctccaactgc tggtcctcta
    32341 atttttcaag caaatcatac ttatcataat aatgcgtata aaatgtacta cggttaacat
    32401 cagctaaatc tgcaatttgt tgcacagtaa tctcttctaa ttggtgttga tgtaaaagtt
    32461 caataaatgc atttctcatt gcaacttgtg attttctaat acgtcgatct atagtcattt
    32521 atatcaagtc ctccccaatg attataaacg ttatgttcat tatcccacaa atctccaaca
    32581 ttgatgattg gcacacaatg tttacctgtt taatataggt gatacaaaca aacagaaaaa
    32641 ggtgataaca atgaaccaac atttactagg aaatccaaaa ttaactgtaa ctcatgtcaa
    32701 tgaagttaaa gccggtatta accacatcgt tgtcgacagt gttcaatatg gaaatcaaga
    32761 aatgattatg gaaaaagatg tcactgtgga aatgcgcgat ggcgaaaaat tatatattaa
    32821 tattttcaga ccaaataaag atggcaaatt ccctgtagtt atgtctgcag atacttacgg
    32881 taaagataat aagcctaaaa tcacaaatat gggtgccctt tggccaacat taggtaccat
    32941 tccgacatct agttttacac ctgaagaatc accagaccca ggattttggg tgccaaatga
    33001 ttatgttgta gttaaagttg cattacgcgg tagtgacaaa tccaaaggcg tcttatctcc
    33061 atggtcaaaa agagaagcgg aagattatta cgaagtgatt gaatgggcag caaatcagtc
    33121 atggagtaat ggaaatatcg ggacaaatgg tgtttcttat cttgcggtga ctcaatggtg
    33181 ggtcgcatca ttaaatccac cacatttaaa agcaatgatt ccttgggaag gcttaaatga
    33241 tatgtataga gaagtagcct ttcacggagg tataccagat actggctttt atcgtttctg
    33301 gactcaaggt atttttgcga gatggacaga taatccaaat atcgaagatt tgattcaagc
    33361 acaacaagaa catcctctgt tcgatgattt ttggaaacag cgtcaagtgc cattatcaca
    33421 aattaaaaca cctctactaa catgtgctag ttggtctaca caaggtttgc acaaccgtgg
    33481 ctcttttgaa ggatttaaac aagctgcatc tgaagaaaaa tggctatatg tgcatggacg
    33541 taaagagtgg gaaagttact acgctagaga aaatctcgaa cgccaaaaat cattctttga
    33601 tttttacctt aaagaagaaa ataacgattg gaaagatacg cctcatgtca tttatgaagt
    33661 tagagatcaa ttttataaag gcgaattcaa atcagcgtca gcgttccctt tacctaacgc
    33721 agaatataca ccattgtatt tgaatgctga aaatcacaca ttgaatcatg caaagattag
    33781 tagcgcgcat gtcgcacaat atgactctga agataaacaa caagatgtaa gttttaaata
    33841 tacgtttgac aaagatactg agttagttgg aaacatgaac ttaaaactat gggtaagcac
    33901 taaagactca gatgatatgg atttatttgc aggtattaaa aagttagatc gtcgtggtaa
    33961 tgaagttaac ttccctgatt ttaatcatat tgaaaatggt caagtagcta ctggttggtt
    34021 acgcgtatca catcgtgaat tagatcaaga aaaatcctca atcgcgcaac cttggcataa
    34081 acatgaaaca gaattaaagt tgtcacaaga tgagattgta cctgttgaaa tcgaattgtt
    34141 accttcaggc acgctattta aacaaggcga aacattggaa gttgttgtaa agggtagtga
    34201 aattgtaatt ggtaatagta ctcctggcat gaaaacacgt tatgaacatg aagaaaccgt
    34261 aaataaaggc atgcacatga tttatactgg tggtaaatat gattcacaat taatcattcc
    34321 tatcgttaat tgatatgcag caattacggt cgcttttgat taaaagtgac atagtgatag
    34381 gactgtataa caagagaaag ccacacgctt ggaatcttaa accaaggtgt ggcccttttt
    34441 attattgatg gctatttaat tttataacac tatcgtattt tctttttcat gaatcatttc
    34501 aataatgaca ttatcttcat tcattactgc tactttaggt gcatggtttt taatttcttc
    34561 ttcattcaac tgtgcataag tcatgattat gactacatcg cctacttcaa caagtcttga
    34621 cgctgcaccg tttaaacaaa ttttaccact acctctttca ccagctatta cgtatgtttc
    34681 aaaacgtgca ccattattat tattcacgat ggctactttt tcatttggca agatgtctac
    34741 cgcttccaat atatctgaat caatcgtaat gctacctaca taatttaaat ttgactcagt
    34801 cactcttgct ctatgaattt tagcattcat cattgttctt atcactttat tcagctccaa
    34861 ttattatatt atctattaaa cgcgcttttg aaaatttaac agctaacgag ataaatatgc
    34921 gtccagttat ttcgtgttgt tctactaatt gaggataact ataaacagca acttcttcaa
    34981 tgcgtccact tatatgtgat tcaagatatt cagtaaccct gtctataatt actttacttt
    35041 gacgttcacc gtcttgatac aacgcttgtg ctaatagcaa acttttactt aaatgtaccg
    35101 cttcttgtcg ttcttgctcc gttaaataaa catttcttga acttttcgcc aaaccatctg
    35161 cttctcgaac gatatcaata ccaataattt caacggcatg attgaagtct tttaccattt
    35221 gctcgacaat agccaattgc tgggcatctt ttttaccaaa ataagcataa tccggcataa
    35281 caatattaaa tagcttatta actactgtta ccaccccatc aaaatgccct ggacgcttcg
    35341 ctccttctaa cacatcagct aatgggccta ctttgacatc aatacctaat tcacctggat
    35401 acatatcttc tactgcagga tgaaaaacaa tgtccgctcc tacttctgat actaattcta
    35461 aatctttatc aatttgtctc ggataagcat cgaaatcttc gtttggacca aattgtaatg
    35521 gattaacaaa tacactcaca attgtaatat catttgtact aactgattcg cgtaccatcg
    35581 ttaaatgtcc atcatgtaag gcacccattg ttgggataaa accaatcgtt gtgcctgagc
    35641 gtttggctgc tttaacaatg tgttgcatct cttttaccgt agtaatcagc ttagtcattg
    35701 ttattaacct cattcataat tttcttttta tacgtatatt cttctgatgg aaatgcacca
    35761 gatttaactt cttgatcgta ttgttttaaa ccatccacac caacactaaa atcagcaaat
    35821 tgcttcacaa atttcgcttt atgttcaaca ccataattta acatatcgtg ataaaccaat
    35881 acttgaccat ctgtaccttt tcctgcacca ataccaatga ctggaattgt taagtgcttg
    35941 ctaatttctt ctgctaaatc atttggaatt gcttcaagta ctaacgcaac tgcaccagct
    36001 tgttctacat ttttcgcgtc taaaataagt tgctccgctg cttctttcgt tgcaccttgt
    36061 aatttatacc ccataacgcc aacactttga ggtgttaatc ctaaatgtgc aacaacagga
    36121 ataccaattg ccgttgcttt ttcaataaat ggtgtaatat gcgctccttc tgctttaatt
    36181 gcatttgcat tcgtctcctg ataaagcttt agagcatgat ttaagtcttg tgtcatagag
    36241 atgcctactg caccaatcgg catatcaaca actacaaatg tatttggtgc gcctcttctt
    36301 actgcacgac cgtgatgaat catatctgct aacgtcactt gtacggtact ttcataacct
    36361 aatacagtca taccaagtga atccccaaca agaatcatat caatacccgc tgcttccact
    36421 tgtttagcac ttggaaaatc ataagctgtt accatagaaa ttttagtttg cttttgtttc
    36481 atatctatta attgacttac tgttttcaat gttattcaac ctctttttgc agtattatta
    36541 gaaatagtat aatgaatctt ttatagaaag gaaagaagaa gatgttatca gttgcgatta
    36601 tcggcccagg cgctgtcggt acaactattg cctatgaatt acaacaatca ttgcctcata
    36661 caacgcttat cgggagacac gccaaaacaa taacatatta tactgtacca catgcacctg
    36721 cacaagatat tgttgtgaaa ggttatgaag atgtcacaaa cacatttgat gttattatta
    36781 ttgcagtcaa aacacatcaa cttgatgcag ttattcctca tttaactcat cttgcacatg
    36841 aggacacgct catcatttta gcccaaaatg gttatggtca actcgaacat atttcattta
    36901 aaaacgtatg ccaagcagtt gtctatataa gtggtcaaaa gaaaggcgat gttgttacgc
    36961 acttcagaga ttatcaatta cgtatacaag ataatgcatt aactcgacaa tttagagatt
    37021 tggtccaaga cagtcaaatc gacattgtat tagaagcaaa tattcaacaa gctatttggt
    37081 ataaattgtt agttaacttg ggcattaatt ctatcacagc actcggtaga cagaccgttg
    37141 caatcatgca taatcctgaa atacgtacac tgtgtagaca gctattacta gatggttgtc
    37201 gtgttgcaca ggcagagggg ttaaactttt cagaacaaac tgtcgatact attatgacta
    37261 tatatcaagg ttatcccgac gaaatgggaa cgagcatgta ttacgacatt gcgcatcagc
    37321 aacctcttga agtcgaagcg attcaaggct ttatttatcg acgtgcacgt gaacacaatc
    37381 ttgacacccc atatcttgat actatttata gctttttacg cgcgtatcaa caaaatatgt
    37441 aagcacatat ataatataaa aagaccttaa cgatgcattt agaaccacct gttcctaata
    37501 tcgctaaggt cttttaatta tattagacta ttcagcttct ctaatttctt ctgccacatc
    37561 tttatagtct attttcgctt taacaaacgt ctcgttattt accgggaaat gttgttggaa
    37621 tgtttcaaag ttttgtatct caacgacaac gtcatccact tcaaagtcaa gaacatgtcc
    37681 accataagct ctttcatcat ccgcaaaatg tatatgaaaa ccagcagacc ctacgccatg
    37741 aaataattct ggtgtaaaaa atccaacaat ggcaccacga atatcttgtc ttttttcctc
    37801 aggttgtctg cgtgctgaat caatcaaacg tgtatatggc ggttgctgag caggcatcat
    37861 tcgtacatgc atatgtttaa atgtgccata aattttaaca gccgaaaata aattctcact
    37921 taacatttca tttttaattt gtgcaaatac atcatcttgt gataattgtt gcaatggaaa
    37981 tgtcttactc gctttaaaat tagtaatcga tgcatacggt actttctcat cgccttttaa
    38041 ttctataaat tctttatgct cgttagcatg atatgccttt ccgtctaaaa atattacttc
    38101 gccatcagac cctgttaacg ttgcaatccc taaattccca tgttctaaca attcattaat
    38161 tgtagctgtg ccttctagta agccagccat taacgtacct aatgtaccat gttgatacaa
    38221 gacattcgtc attccaaaac catctcccta tagttattcc tcgttgtcat gctctacttc
    38281 gtgattaatg cagaactact ttcctttata ctcaatgacg cctcaacatt tttattcatg
    38341 tcactaaatt ttcacgagtc ataatacttt attcaattat tattttacgc aaaagtattg
    38401 cctctatcaa tgcttgctaa aaaaatggac ctatcagtgc cacgtgttac tgcaacacta
    38461 atagatcctc ttaccattaa aatatttcta attaatactt aaaataggct tagtcttcta
    38521 ataaatattt aattgaatca aatgtatctt ctaatgtttt aacagattta tcgaacaacg
    38581 cttgttcttc ggcacttaat ggcatttcat aaattttaac tgcgccatgt tggttaacta
    38641 atgttggtac acctaggtaa acgcctttgt gaccaccata ttgaccatct aattgtatag
    38701 aaacatttaa gacattattt tcattattta aaatggcttt tgaaatgcgc atcaatgcta
    38761 atgcaatacc atagtatgtt gaccctttag cttggataat ttcataagca gcgtcacgtg
    38821 tattcacata aatttcttcc gctttagctt cgctaccagt ttgttctttt aatgtgtcat
    38881 atactgaaat acctgctaca tttgcttgtg accaaactgc aagttcagta tcaccatgct
    38941 cgccaataat actagcgtca acacttgaag gtgcaacacc aagttcttgg ctaattaaat
    39001 attgtaaacg tgcactgtcc aatacagtac ctgaaccgat aacacgctct gctggtaatc
    39061 cagtatattc ttttacaaat cttgttaaaa tgtctacagg gtttgccgcg attaagaaat
    39121 atccatcaaa gccactatcc ataacactct taacgatgct cttcataatc ttagtatttt
    39181 tttcaactaa ttgtaaacgt gtttcacctg gcttttgagg tgcaccagct gtaataacaa
    39241 ctaaatctgc atctttacag tcttcgtatt cacctgcttt cacatcaact ggtgaaggac
    39301 tgtggactgt accatggttt aaatcttgaa catctgcttt tactttgtct tttgcaatgt
    39361 caataattac aaattcatca gcaacacctt gcgtaaccat tgcaaaggca tagcttgatc
    39421 ctacagatcc atctccgatt aatacaacct ttttaccaaa tgttttcata attatgtctc
    39481 ctaacctaat tgaatttatt ttacacgtga ctacacgtaa aaatatctat actgaaatta
    39541 aagattaaca attttatttt attataatat tatgctacca aattacaaac ataaaactga
    39601 gatgtaagcg gagtcaaatt acactctatc gccaataaat attgatatct caagctttag
    39661 cacgtaattt gaaatttgtg acaattttta gcattctaaa attgcgtaac gacgctattt
    39721 aaaggcttta acaaactatt gagctttcta tttttttcat tttaaaaagg atggctattc
    39781 gacttaaatg acttactata taacctccta ttttccgcct taccctcatc atatttgatg
    39841 catcatttta tagtcaaaga attacatttc atacaaacta gccaatcaga cataaacatg
    39901 aaattgattt taaaaatata atgtgcaaaa ttgttagaaa attagcaaaa aatagtactt
    39961 attcatttaa tatctgttat aatcagtttt atcatttaaa aaatctatgg atgttatcaa
    40021 gacaagtcat taatacgtaa cataaagttg tgatttttca gaatttttag acaaaatatt
    40081 gattatttgt gctatgatat aacatattaa aaaacaccgt atagaattgt ttatgaatgt
    40141 ttgggaggaa agtttatggg aagttttttc aataaaatag cacgaaaaga ggatccggct
    40201 atctatcaaa ataaagatgg tcatttaaag cgtacacttc gggtgcgtga tttcttagct
    40261 ttaggtgtag gaacaattgt atcgacatct atctttacgc tacctggcat tgttgctgca
    40321 gaacatgcag gaccggccgt tgcgttatca ttcttactcg ctgctattgt tgctggttta
    40381 gttgcattta cttatgcaga aatggctgcc gctatgccat ttgcaggttc agcctattct
    40441 tgggtcaatg tattatttgg tgaatttttt ggatgggttg ccggttgggc tctattagct
    40501 gaatatttta tcgccgtagc ctttgttgca tcaggattct cagcgaattt acgcggactt
    40561 gtgaaaccaa ttggcatcga attacctgca gcattatcaa atccatttgg tacaaatggc
    40621 ggttttatcg atattattgc tgctatcgtt attttattaa ctgcattatt actatcacgt
    40681 ggtatgtcgg aagcagctcg tatggaaaat attttagtta ttttaaaagt attagctatt
    40741 attttatttg tcatcgtagg tttaacagca ataaatgtta gtaactatgt gccatttatt
    40801 ccagaacaca aagtaactgc tacaggtgac tttggtggat ggcaaggcat atatgctggt
    40861 gtttcaatga ttttcttagc gtatatcggt ttcgattcta tcgcagcaaa ctcagcagaa
    40921 gcacttgatc ctcaaaagac aatgcctaga ggtattcttg gttctttaag cgttgctatc
    40981 gtattattta ttgctgtagc acttgtgtta gttggtatgt tccattactc acaatacgca
    41041 aacaatgctg aacctgttgg ttgggcttta cgtcaaagtg gtcatggtgt tgtagcagct
    41101 attgttcaag ctatctctgt tatcggtatg tttacagcat taattggtat gatgttagca
    41161 ggctcacgtt tactttattc atttggacgt gacggcttat taccttcatg gttaagccac
    41221 ttaaacgaca aacatttacc taatcgcgca ctagttatac ttactattat tggtgtttta
    41281 attggttcta tgttcccatt cgcattttta gcacaattaa tttcagcagg tacacttgtt
    41341 gcatttatgt tcgtttcatt agcaatgtat cgtttgagaa agcgtgaagg taaagatcta
    41401 ccaattcctg catttaaatt acctttatat cctgtgttac cagcaattac atttgtttta
    41461 gtattgctag tattctgggg attaggattc gaagcgaaat tatacacttt aatttggttt
    41521 attgttggta tcatcctata tttaagttat ggacttagac attctaagaa aaatgacgta
    41581 gctgaatacc acccaccaaa ataaaaaata aaaaaaatca tttcccatct gattaacatc
    41641 cagatgtgga aatgattttt tattttatat taagataatc gctattacca accttgtcca
    41701 gatatgtcat attgatctaa gttacctgct tccaaagcag tcagtgcatc ttctatagtg
    41761 tttaacgccg tgtctaattg ctcatatgtt attaccaatg gcggttggaa tcgcaacaca
    41821 tttcctgcta cagctataat aactacgcca tgctcaaagc agtaattaca aattttaagt
    41881 gccgcactgg catcacgtgt tttgagtttt ttgtcggaaa caatatcaat accaatgctc
    41941 agacctttac ctctaacatc gcctacacta ttgtatttag atacccattg atccattcgt
    42001 ttcctaacat attccccttt ttccgcacta gcctgaagaa gcgactgatc ttcaatcatt
    42061 tgaattgtgg ctaatgcagc ttcacaacta actggattag cacctgttgt aaataaatgt
    42121 gctggtgctt ctaaacaatt cataatctct ttgcgtccaa caattgctga cataggcata
    42181 ccacctgcta aggattttcc gaaagtgatt aaatcaggcg taaaattaaa atgcgagact
    42241 gaactccatg tacctgttct cccaaaacct tgttgaatat catcgacagc gattaaaata
    42301 ccatgttcac gacaaatctt ttctaacgct tcaaaatacc ctggaactgg ttctaaaagt
    42361 ccaccatcgc cttgtatcgt ttcaataaca atacatgcta cttcgtcagc aggtacatac
    42421 ttcgcaaaca tttcttttaa gggtgctaaa tattcttcta ctgaattagc ttgtggctgc
    42481 tcgtacatac cacgatattt atctggaaac ggaatatgat aaaaaccatt caataacgga
    42541 ccataatgtt tgcgcatatt taaactaata gctgacatag acaatgagcc aaaagttgaa
    42601 ccatgatatg cattagtgaa actaatgata taaggacgcc ctgtatatgc tctggcaaat
    42661 ttaatgatgc catcatttgc gtctgatccg gttaatccga aggtcactct tttttcaaaa
    42721 tctccaggtg caatctcaca aagcttctta gctaaacgta ccaacggttc atgatacata
    42781 tatgctggtg tataatgaat aaatttatca acttgtgcct ttatcgcttc tgttacttct
    42841 ctaggtgcat gacctacgtt ttgagaactc gcactcgata acaaatcgat atatgtcttc
    42901 ccctcaatat caaccaatgt tgctccatat ccatgatcaa tcactaacgg ataatattta
    42961 atacgtcctg atttcgcaaa ataatgttca tcctcttgaa ttaattgatg tgctttactc
    43021 atatacattc ccctcccatt gacagtatta atgtgcaagc aaagtggcct ctcttaaatc
    43081 gattataaaa tccgatttaa aatcctgctc aaataccgtc atgcctctat gaatactatc
    43141 caatatacta ataaaattca gatttgtaaa tatattctga taattaagaa ttttatatca
    43201 atctactttg tttatgcgca taattttgtt cgattaggga attgtttact atattccatt
    43261 tgactctgag gtgatacgaa tgcgtttaaa agtattgttc catttcattg ctgctatttt
    43321 catatcattc atgttgctat ggatgaccat gttgtttgac ttaataagca atcaatcaca
    43381 tttaaaagct ttattattga atttagattt tctaattcct tctgacaata caccatatat
    43441 tctagaaata atatgtcact tactaattgg tagcgtcatt tattttgtat ttgtgttgct
    43501 gtttcatact tctaaaaggc tttactatct atgttatatc cctttatttt tcttatttat
    43561 agcattgtat ccctttttag tatttattgc gcaacgtcca atttttcaat ttagtgtgac
    43621 agaattaatc ggttggatta ttacacatat tttctttatg tcattaatgg cgttagtcat
    43681 acctagaata aaataatatt tttaattaaa ctgacaatta tttttcacat tttatacact
    43741 tttcaaggtt tatgacttga gaaatgttct catttttatc atactggagg tgtattgaaa
    43801 agttaaggag tggaaagtat gaataaagag caattagaaa aaatgaaaaa tggaaaaggc
    43861 tttattgccg cattagacca aagtggtggt agtacaccaa aagcacttaa agaatatggt
    43921 gtaaacgaag atcaatatag caatgaagac gaaatgttcc aacttgttca cgatatgcgt
    43981 acacgtgtgg taacttcacc ttcattctca ccagataaaa ttttgggtgc tattcttttc
    44041 gaacaaacaa tggatcgcga agtagaaagc aaatacactg cagattactt agctgataaa
    44101 ggtgttgttc cgttcttaaa agtagacaaa ggtcttgctg aagagcaaaa tggtgttcaa
    44161 ttaatgaaac caatcgacaa cttagacaat ttattagacc gtgcaaacga acgtcacatt
    44221 tttggtacaa aaatgcgttc taacatttta gaattaaatg agcaaggtat caaagacgtt
    44281 gttgaacaac aatttgaagt tgctaaacaa attattgcta aaggtttagt tccaattatc
    44341 gaaccagaag ttaatattaa tgcaaaagac aaagctgaaa ttgaaaaagt attaaaagct
    44401 gaacttaaaa aaggtttaga tagcttaaat gctgatcaat tagtaatgtt gaaattaaca
    44461 attcctactg aaccaaactt atacaaagag ttagctgaac atcctaatgt tgttcgtgtc
    44521 gttgtattat caggtggtta cagcagagaa aaagcaaatg aattacttaa agataacgct
    44581 gaacttatcg caagtttctc acgtgcatta gctagtgatt taagagctga tcaatctaaa
    44641 gaagaattcg ataaagcttt aggtgatgct gtagaatcaa tctacgacgc atctgtaaac
    44701 aaaaactaat ttatacatta aactattttt tgatataaac tactaaaaag gactcataca
    44761 tgtgctttaa aggcattgtg tatgagtctt ttttgattaa agttgatttt gccgtatgta
    44821 ttagattacg taaaaaaatc ggacagctga aaaatcttta agatttctca gttgtccgat
    44881 tttaaatata gctattagaa tttagttttc gtagtaacct aattctaagt cttttgaagt
    44941 ttgtttacgg atttttctca ttaatttttc gtcttcaatt aatgattcac cgtatgatgg
    45001 aatcattttc ttaattttag gtgcccattc agttttgtat tcagggaagt tacgttctaa
    45061 tacttctaac gcaactgaaa ctgaagttga agcccctggt gattcaccta ataatgcaat
    45121 tacagtgtgg tcttgtgagt taaccacttc tgtaccgaat tggatgaatc ctttaccgtg
    45181 ttcaggtgta tctttgataa cttgtacacg tttaccagca gtgtataatt gccaatcttc
    45241 attacgtgct tctggataga aagtacgtaa gtggttcata caaccttctt ttgtcataat
    45301 tacttggtca aatgagtatt taattaaagg taagttttta actgctgctg ctaataaagt
    45361 tgtaatgttg tatgttttaa cagacttgaa taaatctaag ttagaaccat ttttcaagaa
    45421 tttaggtcca acattagcaa atggtccaaa taataatgtt ctttgaccat caatgtaacg
    45481 cgtatctaaa tgaggtacag tcattggtgg tgtaccaggt ggctctttac cataaacttt
    45541 ggcatcgtgt tgttcaataa cttgtgggtt tgtacaagct aagaattgac cactgatagg
    45601 gaatccaccc aaatgtttac tttcagggat acctgttttt tgtaataatg gaattgctcc
    45661 accgccagca ccgatgaata cgtagtcagt tacttgtttg aatttctcac cagttaggcg
    45721 atttttaaca gtaacttccc attgaccatt tgataattgt tcaaaatcaa caacttcatg
    45781 gttaaattgc actgtagcat ttggatgtgc ttcaatgctt ttagccattt tacgtgttaa
    45841 ttcaccgaag tttacatctg taccttcgtc aattttactt gccgccatga taccagggtt
    45901 atcttcacgg cctttcatca tcaatggaat ccattttttc attacttcga tgtcttcagt
    45961 atattcgata ttatcgaaca tagggaaagc tttcatcgct tcgtaacgat cttttaagaa
    46021 tttaacattg tttttacctc taacataact gatgtgtggt aatggattga taaattctct
    46081 tgggttctcg atgctaccgc ttttcactaa gtgaccccag aattgttttg aaatctcaaa
    46141 ctcttcgtta atcactttcg ctttttcgat gtcgatagaa ccatcaggtt gtaaaactgt
    46201 gtagttcaac tcacataatg ctgcatgacc cgtaccagca ttatttcttt cgtttgaact
    46261 ttcgattgca ggacgatcca agcgttcgta aacgtggata ttccagtctg gctcaatttc
    46321 ttttaacatt gaaccaaatg ttgtgctaag tacaccggct ccaattaaaa cgatgtcttt
    46381 actattagac ttagccattg gtttcacctc tccaaaaatt gtaaatgtgt atcatcaata
    46441 tgaaagttac ataaaactga catatttctt taaaatatca acgccattga taacttcctg
    46501 ttttaattga tacgctgtaa caaaatacta tagttagtgc ttacatgtat atgttaaagc
    46561 aagcagtggt aaatgtaaat tataattatt cattaacttt gcaatatatt aaatctttta
    46621 ttcatagaag ataaatatca aatcaatcat aattatttga caacaaatag ctaacgattg
    46681 ttttaatcta catttggctt atagcatttt aaacctatac tctattttga tacaatataa
    46741 gtgtaaaatc aatcataaaa aggatattca atatctgcat ccaagaaaaa cattacaatt
    46801 acctttaata ttgaaaatta ttttgaatct tgcacttata ggtattgtaa ttatcgttct
    46861 aacacatact gagtttcaac ttaatgccgg tgatagtaat gtgtttttat taaactggga
    46921 tattcgaggt ttaacatctt atattttctc attcttaata ctggttgcgc ttgtttctaa
    46981 actaatttta atatttataa ctagacgaca aagaacatca taaataaaca agcagtccag
    47041 tgcgtatcat cataccttac gcattggact gctttgctat gtttattgat tgctatcttt
    47101 ttgttgttgc catttttcta tttcagcatc tcgtagttca acacgacgaa ttttacctga
    47161 gtttgttttt ggtaagtcgt caacgaattc aatctctctc ggatatttat atggtgcaac
    47221 ttcattttta acaaattgtt gtagttcttt aactaacgta tcatcacccg cagtatggtc
    47281 ctgtaaaata acgaatgctt taacaatatt tcctcgtata tcatgaggtt ttgctactac
    47341 agcacattct ttaacggctg gatgattcgt taacgcatct tcaacttcaa aaggcccaat
    47401 cgtatagcct gaactaataa taatgtcatc tcgacgtcct tcaaaccaga aataaccatc
    47461 atcatctaca tgagctaagt caccagtgat gtagtattta cctgtttgcg ctttcgccgt
    47521 acgttctggc tctttataat accctttgaa aagtgctggc aaatcaagtg gtactgcaat
    47581 attccctttc gtattagcag gtacgctatt cccctcatca tctactacag tgaccgaact
    47641 acccggaatg cctttaccca ttgatccaat cctctgtggt gtatctttta aaaagcctat
    47701 aagcaaggta ctttcagtct ggccatatcc atctcttaca gttaaattaa agtatttctt
    47761 gaattgttca actacttctc gatttagtgg ctcacctgca gaaacggcac tatgtaaatg
    47821 cgttaagtca taatcattta agttctgtaa tttagccatc atacgatatt ctgtcggtgt
    47881 acaacataaa acattaattt gatatttttg aagcaattct aagtatgttt caggactgaa
    47941 ccttccatta aatacaaaag cagttgcacc tgaacctaat acagataaga aaggactcca
    48001 tacccatttt tgccaacctg gtgctgctgt tgcccaaact aagtcatctt cattaataca
    48061 taaccaatgt tttggtgcca tttgtaaatg tgcaaatccc catccatgac aatgtgtaac
    48121 ggctttagga ttgccagttg taccagatgt atatgacaga atcgccatat catcacgcgt
    48181 cgtatctgcc atttctagtt tgttacttgc gttttctttt tcagcttcaa gtgaaatcca
    48241 tccatctttt tgaccggcaa taacaaattt agttaacgca tcatattctt taattttttc
    48301 aaattcaact gtgaatggct ctagtgcaat aactgcatta atttcaccat gtgtgatacg
    48361 gtattgtaaa tctttagttc ttagcatttc agaacatgga atgattgcaa cacctaattt
    48421 taaagcagca atatataatt catacgtcgc aatagatcgt ggcatcataa tgagtacttt
    48481 atcgccttta gataaaccgt gcgatgctaa aacattacct actttattag actgttcaat
    48541 gagctgttgg taagtgactg atatatcttc gccttcagta ttatgatata aaattgcctt
    48601 tttatctggt atgtggctat atttttcgat ttccgaaata atgttatatt tttcaggcgc
    48661 gaatagagct gacttttgca taactaactt cctttcatac atccactttt cctgtgatga
    48721 acattgtaat tttataaatg aattatatac atcatacgcc tatctttaca gaattttcaa
    48781 ttaaataggg ttaaatacca aagtcctcga cactacactt tgacatgacg tagcattcaa
    48841 ggactttcaa atgattgagg gttgatatct cgggctagac catatcagct aattcaatac
    48901 gaatattgta tgataattca cgattaatta tttttacatc tgcacctttc gaagtgccac
    48961 gatgcttgtg tgtatgcttg tactcagctg aattttgcca atgataaaat gcttgcctat
    49021 tttcccacag cgtaataatg atatagtgtc taccagctgt tctaggtctt aaaaacctta
    49081 atgctttaaa tccatcaacg ttttttaaat gcttattacg tcgcaaaaat ttttgctcga
    49141 atgtttcttc ttgtccttcg ttaatatata aatgatttaa cacacataat gaatcatttg
    49201 ataaatcatt tatcgcttct agcacatcgt aatatgcagt gtcattattt ttttgtattg
    49261 tgagacaatc atccagttct tctattacat aacttctata ttcatcataa attttcataa
    49321 taaatgcctt catttcattt atatttttgg tcatattact ttatatctat tactaattca
    49381 ttcccgtatt tattaattac aatcatagtt tggcttcttt ttaaaagata agactttgta
    49441 aaaagtatta atatttcatg caaatggggg acaggagtcg cccactattt ttgtgtcttc
    49501 aatttcatga tcattattta acattagtca tgaaaatagc cgacaacttc atctgtgaaa
    49561 tcaccggcct tttattttag ctaactttat ttctgatttt acgattttaa ttgatcatac
    49621 agagaaagtg atctttttac aatttctaaa aactcatgat ctatattgga catttgatga
    49681 aaataagaca aaatgttttc tgttagcttc tcttgttttg ggaatgaatc atcttcttta
    49741 atccaaatcg ctaattcgcc taatggtgtt ttatcatctt taaatgtttg tatatattcg
    49801 taaaagctca tagtattcct tctctcaatt tacttatata aatcctacca cgaaagcttt
    49861 caagaaaaca caattaaatg tctatttagt gaacttttta aggttgtgca ctcttttaat
    49921 gtctgccaat taggtcaatt aatcatcaca atgtacaatt aactctattt tcagttcata
    49981 tactcacaca ccgtttttga acaacacatt aacttctcat ttagataaaa cgcaaaaaag
    50041 cctggcacca atacaataga tgccagacta agagtctact atataaattt atttagcgta
    50101 tggttttact tcgattgcac cttcattttc atcatgaaca ccatgcttat aataatcaat
    50161 atattgtggc tctaaaggct ttctgccacg tataatgtct gctgcttttt cagctaacat
    50221 taaaacaggt gcgtgtatat tgccatttgt cgtacgtggc atagctgatg catcaactac
    50281 acgtaaattt tccataccgt ggactttcat tgttaacggg tcaactactg ccattggatc
    50341 tgaagcagga cccattttag cactacaaga tgggtgtaat gctgtttcac catctctacg
    50401 aacccaatca agaatttctt cgtctgtttg cacttctggt cctggtgaaa tttctccacc
    50461 attgaatgga tccattgctt tttgagataa gatatttctt gctacacgaa ttgcttctac
    50521 ccattctttt ttatcttctt ctgttgataa ataattaaag cggatacttg gtttttcgaa
    50581 tggatcttta gatttgattt tcaagctacc acgagagttt gaatacattg gtcctacgtg
    50641 aacttgataa ccatgtgcga ccgctgcctt ttgaccatca tatcttacag ctattggtaa
    50701 gaaatggaac attaagttag gataatcaac ttcgttattt gaacgtacaa atccgccacc
    50761 ttcaaaatgg ttagatgctg ctgcacctgt acgtgtgaaa atccattgta aaccaataaa
    50821 tggcatgcgc ttgatatcta agcttggctg taatgataca ggttccttac atttatgttg
    50881 aatgtatacc tctaagtgat cttcaaagtt ttcacccaca ccaggtaaat gaacacgtgg
    50941 ctcaatgcct tttgatttta ggaactctga atcaccgata ccagataatt gtagtaattg
    51001 tggcgtattg aatgccccac cagacaaaat gacttcatta gcatcgatgg tatgtagttt
    51061 gccatttttc ttatacgtaa caccagttgc tcttctacct tcataatgaa tttcagttac
    51121 aaaggcacgt gtttcaacgg ttaagttttt acgcttcatc gctggatgta aatatgctct
    51181 tgaagctgac attcggcgac cacgatgtac ttgactatcg aacggtccaa aaccttcttg
    51241 tctaaatcca ttcacatcag gtgttttatg atagcctgct tcaacacctg catcaaagaa
    51301 tgactggaat aaaggattcg ttgctggccc tcgttttaac ttaattggtc catcatggcc
    51361 tctaaattta tcataaggcg ctgcaccgta tgttttttct aattttttaa aatacggtaa
    51421 acagtgcgca aaatcccaag tttccatacc ttctggttct gcccagcctt catagtccat
    51481 tggattacca cgttgataaa tcatgccatt aatcgaactt gatccaccta aaactttacc
    51541 tcgggcatgt gctactttac gaccgcccat atgtggttct tcatctgttg aataaatcca
    51601 atcgtaaaat ttattgcctg aagggaacat taacgcagca ggcatttgga taaataaatc
    51661 ccaaaaataa tcactgcgac ccgcttctaa tactaagact tctttatctt tatcttcact
    51721 cagacgatta cctagtacag aacctgcact gcctcctcca atgatgacat aatcatatga
    51781 tttgtttttg ttactcatcg ttatgacctc caaagtttgt tgtatttcaa agtgagtatt
    51841 gcatgaaata tttgttagtg gctgattggt tatcgttgaa ttgctaaact tagctaacca
    51901 aaacactcgc ttttcctttt acgattgtta ctaacagtaa tgaaataatc tatgattcat
    51961 tttgcgttta gtccaaatta tcaatcaaca tttcgccact tatacaaaac aaagtgagtg
    52021 tcttgtgttc ttacaatggc actcacctta tctaattttt atttgctaaa ccaattcact
    52081 aattgtggat ttgtatttgt taaaatgtgt tttgaaacaa ggtactcttc taagccttct
    52141 ttgcctaatt ctctaccgat acctgattgt ttgtatccac cccatggcgc ttgtgcaaaa
    52201 tatggatgga aatcattaat ccacaccgtt ccaagtttca acttgttagc aacgcgttgt
    52261 gcttttccaa tatctttaga aaatacagca cctgctaaac catatataga atcattcgct
    52321 aattgaatcg cttcttgttc agtttcaaag ccttctacag taacgacagg tccgaaaacc
    52381 tcttcttgta caatacgcat tgacgtatca caatttgtaa tgactgttgg ctcgaagaat
    52441 agaccatctt ttaaatcatc tctatctgga cgtttaccac caacagcaat tgttgcgcct
    52501 tctgctttag ctacatccat ataagattcg atcttattac gatgttctgt tgaaatcact
    52561 ggtcccattt cagtatcagc atcaaaacca ttacctaatt tgattttttt cacgcgatca
    52621 ataagtgctt gctcaaattt gtctttaata ctgttttgta ctaatattct tgatcctgct
    52681 gaacaaactt gacctgcatg gaaatatcca ccatttaacg cttggtctac tgccaattca
    52741 aaatcagcat catcaaagat aatgtttgga tttttaccgc caagttccaa ggcaatattc
    52801 gtaacattat tagcagcatt tttcataata tgcttaccag tctcaatgcc acctgtaaat
    52861 gatacaaggt caacctcttt atgacctgac attacgtcac caacttcaga acctgcacct
    52921 agaataagat taattgttcc tttagggaaa ccaacttctt ccattaattc aaaaacacgt
    52981 attgttgtta atggtgtaat ttcacttggt ttcataacta gtgaacaacc cgtagcaagc
    53041 gctggcgcaa ttttccatga tgcttgtaat aacggataat tccaaggtgt aatttgtgta
    53101 actacaccta ctggttcttt aacaattttg ctttctgtat ctggaattgg tgaatcaatc
    53161 atttcgccac cgtctttatc tgctaatcca gcaaaataca taaacacatt atgaatatca
    53221 tccatatctg catatgattc ttctaacgtt tttccagtat ctaatgtttc taatcgtgct
    53281 aacgcttcgc gatgttcttt aatcttgtcc gcgatggcac gtactttttt acctcttgtt
    53341 tcagcagttt cttgcgacca ttcaccagac tcaaacgcac gtcttgcagc taagattgca
    53401 cgttctgcat cctcttttgt cccttcagaa accgtaaata tcacttcttg attgtaagga
    53461 ttgataatat ctcttgtatt tttattcgcg ctttcaaccc actcaccatc aatatattga
    53521 cgctgagata aatgttttaa aagttccatt caacaacctc cgttaagttt gttaaatttt
    53581 tatttaacga attttacaaa cttatgttag catagaaatg aaaaatgtgt caatttacaa
    53641 aacaactcaa gaattaagtt ttgtaagttc gttagatttt tatttaacga aacatttaac
    53701 tttaatgctt taaatatttt taatgtcttt ttttagaaaa ggttttataa tgtattagta
    53761 tttattttaa cgtgaccaaa atgatgtacg gaggtgcaat gatgactcgt tctcagaaca
    53821 aacagcaaca attagaacaa gcaaaagata ttgtaattaa ttcaattgga cagacaatgg
    53881 atttgtatgg cactaataga agtgtaggaa acttatatgg cacaatggtg tttgaaggca
    53941 gtatgacgct tgatgaaatg cgtcatcaat tgcaaatgag caaaccaagt atgagtgctg
    54001 gcgttaagaa attgcaggaa tttgatattg ttaaacaaca atttacgaga ggaagccgca
    54061 aacaacattt tatcgccgag aaagactttt tcatcttttt ccgaaacttt ttcactaaaa
    54121 aattccagcg tgaaattgac atcaatgtcg aagcagtaaa agatgctcaa gctattatca
    54181 attcattact tgaaagcagt gatttaactg aagctgaaac acaagaggca caaaaaatta
    54241 aatctcagct tgatcataca catgtttatt acgaatggtt agaacagtta accgaagcga
    54301 ttgaaagcgg tgaaattttc aaatatttcc caattccaga acagccttct gattctgaaa
    54361 attaaaattg actcaaacaa cgacaatgat gattatgcat gataaaaaca atgcaatagg
    54421 tccacgaaat aaacggtaat ttctatctca ataaatagaa attaccgttt ttgttttata
    54481 tgacaatcaa ttatggaggt attttctagg gctttcttag attcgttaca ttgcttcgcc
    54541 aatatatttc aattagctat tctcgccctc acgtactcta tcaataatat tttgtatttg
    54601 ttcacgtttt tcatattcat tttctacaaa ataaactaac ggttcatctt tgatagtaac
    54661 ccaaggtaca agtacatcaa aatctaattc tttaaccatc tctaaacttt gaatagattt
    54721 ttctctatca cttgaaccta atataactgt acgccatctc ttcccttcaa aacatataaa
    54781 atctccagta aataagtaac ggtgatgacc atcatcccat aaaaatagtg tcgtccctgg
    54841 tgtatgtcca ggcgcgggaa taacttctaa atccttatcc aacattacac gattactaaa
    54901 ttgtttttga acagaaatct tatgtttcag tgcagccaca tcattttcat gtataaagta
    54961 aggggcttca acttgattag aacctcctat agattcatga tcatgattca tcaatacttt
    55021 tgaaacacca cctaatgatg caatgtcttg ttgggagtca cctaagtgtc cggaatgata
    55081 gataacaata ttacctgttt cacgttttaa aataaaagat ttaaatagaa atcgattatc
    55141 aaaaggcagt tccgaagtag gtgtcgcata taagtttttt gtgatggatt ctaaactgtc
    55201 atgtaatttg gactgtttat ttttaaaatg atattttcac aaaattcatt tcgtcgtccc
    55261 agacccgctt tgaattataa attatcgtct tgttcttctt ctgatacttg aacgattcgc
    55321 aatgaacgac gttcaacttc ttttaatttt tcagcacgcg tttcaagttt aattctatcg
    55381 cgccctaaaa tgattaaaaa tgatatcatc atgaaaataa aaataacaat taatggcaca
    55441 cttgccagta ttgaagcagt tttcaatact tctaatgcac gttcaccacc aactagcatc
    55501 aatgaaaatg gcaataagca caatgcaaat gcccagaata aacgattggc acgtaatggt
    55561 tcgcctacca cttttttctg agatgctgcc gctaaaatat atgaacccga atcaaatgtt
    55621 gttgctaaga ataagaaagc agatactaag aatagtacaa tcatcaatga tgggaatggt
    55681 aaatgatgca ccacttcaat aatggttgcc tctgtaccat gtgtatttaa atattgtgtt
    55741 acattaaact gtccagaaat ttgtaaatac acagcatagt taccaaaaat accaaagaat
    55801 aatacgcatc caagcgttcc ataaataatt gttcctagca cgacttcttt aaggcgtcga
    55861 ccttttgaaa ttctagcgat aaataaaccg ataaatggcg catatactaa ccaccatgac
    55921 cagtagaata ttgtccagtc ttgtgggaaa ttcgtttctt ttcgaccttt aataccaccg
    55981 aatggttcta accatgttgc catatgaaag aaatctctca acatatttcc gaaccctgtc
    56041 actgtcgttt ccataataaa aacagtcggt ccaataataa atataaaggc taaaagtaca
    56101 aaggatagcc aaacgttgat atcacttaac ttttgaatac cttttttcaa tcctgtatat
    56161 gaactaatgg caaatataac cgtgattgtt aataaaatgg ccgaacgtaa aatcatattt
    56221 ttaccatcta aaccagttaa tctttctatg cctgcagaaa ttaatggcac acctaacgct
    56281 agtgatgttg ccgcaccacc tagcaatcca aagataaata agatatctac aactttacct
    56341 acaaatttat ctgtttgacc ttttaaaatc ggacgacaag cttgactaat tttatacacc
    56401 ggttgttttt taacaaatac taaataacca attggtaatg ctggtagaac ataaatagcc
    56461 caagcaattg gcccccagtg gaacatacca tattgcgtcg catattggag tgcttcatca
    56521 ctcatacttt tcgcgccatt tggtggaact tgatagtaaa aagcccattc aataacgccc
    56581 cagtataaaa tatcagagcc tatgcctgca caaaacagca ttgccgccca tgtaaatgta
    56641 ttaaattctg gtttatcact tgctttacca agtgtgacat taccatattt accaaatgcg
    56701 atatacatta caaagcaaaa aatcgccagc cccataaata aatatatcga accaattgaa
    56761 tcagaaatgg cactattaat accagtgatg atatcttcac ttgcttttgg aaaagccatc
    56821 ataggtataa ctgcaaaaag aagtacagct actgtcccta taaaggtcgt ccagtccata
    56881 actttctctt ttttcaattg tgctccccct aattattaat tttatgaatc ctgtttcgat
    56941 ttatctcaaa atgtaataat tatattgatt cacaaaattg acaataacta acatttaata
    57001 ataatgcaaa tttcatacaa tttgaaactt ggcaattatt gaatatttat ataatttttc
    57061 ccgtaataaa caaaacctta atagcgctaa aataacagtg ttaagttacg atttaacgaa
    57121 tttaacaaat tttactagaa tggcatttaa gaatatttat acgttattaa cgaatattta
    57181 tttattgtaa aacgctacca aaaagttaga cttccttccc actaaaatac cacttttctc
    57241 tttcaacttt tttaaaaaac ggatatgcaa cttttagtat tggtatcaaa atgattgtta
    57301 ggtcatattc tatcaatata tttttataaa gaattgcttt tattaacttt caattatgta
    57361 cctaacctaa aaagaagcca aggcaacgaa tgttaccttg acttctaata catattcaac
    57421 taactatata ttcaatcata cgcgcatgcg agagtgattg ttgtacatct ataatgcgtt
    57481 gatttaaaga acctttatat ggtaaatcag gtttgaataa gtgttgtata aatagaccat
    57541 ctactaaaac gtcaatgtat gataataact ctcgacgttc tgtacaatca tttgctaaat
    57601 attcatataa aaatccagtc catacccaaa ttgtctttgt atttccaaaa cgtgctcgaa
    57661 atgctttgac aagatttaat gtaatatcca aattacaaaa tggttcgcca cctaatagac
    57721 ttagcccaga tatataatca tgatcgcaat catctaatat ttctgctaat atttcatcag
    57781 tgtatttctc gccatatctg aacttttgtg aggctttgtt ataacatcca acacaattaa
    57841 atggacatcc tgatacataa acactgcatc ttactccttc accgtcaaca aagctatttg
    57901 attctatttt agcaatataa ccttgtcctt gtttaatgtc taaaagtatc attctttagg
    57961 cgctttcata tgttttactc gtgcgcaaat ttctttatga cggcctttaa ttactggacg
    58021 ttgaactgga ttgcctaggt aaccacatgt tcgtttaacg acatcaactg ttttaggatt
    58081 atcattgcca cagttcgggc atttaaatcc tttttcagtt gcttcaaaat ctccatcgta
    58141 atcacattca taacaatgat caatcggaat atttgtacct aagtaaccaa ctttgtcata
    58201 agagtagtcc cataccgctt ctagtgcttt caaattgtgt tgcaatttcg gatactcaca
    58261 atagtgaatg aaaccaccac tcgcataata aggataatct ttttcaaaat ctaacttttc
    58321 aaaaggtgta acatctttac gtacatcata atggaaagag ttttgataat atcctttatc
    58381 tgtaatgtct ttaatatctc caaatctctc ttggtctaaa cgacaaaaac gatccgttag
    58441 cgattcactc ggcgtactgt aaatactgaa ccaaatgtca tataattctg tccatttcgt
    58501 ttgataacgt ttcatttctt taagaatttc aagcgtaaat gcttttgctt cttgagatgt
    58561 ttcccagtct ggaccataga aaacagtagc tgtttcatac aaccctatat agcccattga
    58621 aatcgttgca cgtttatttt taaataactc agcaacatca tctgtttctt ttaatttata
    58681 gttaaatgcg ccacttttat ataaaatcgg tgcgttattc ggtacagcat ctttcaaacg
    58741 atttatacga taaagtaatg catcatgtaa cacatcgata cgttcataaa agatttccca
    58801 gaatttcgtc atattaccgg cagattctaa tgccattcta ggtaaattaa gtgtaacaac
    58861 accaagatta caacgaccat tattttcaaa atgaccttcc gcatctttcc aacttggtaa
    58921 aaatgaacga caacccattg gcgctttgaa atcacctaat atttctacga gtttgtcata
    58981 atttaaaata tctggataca tacgtttcgt tgaacacttt aatgctagtt gtttaatgtc
    59041 atagttcgga tcttgcggac taaagttggt tcctttttta attgaaaata caagtttcgg
    59101 gaaaatcgct gtcgtgcggt cttttcctaa gcctttgata cgagtattta agatagcttg
    59161 ttgaattttg cgacttaaat gatctgtacc taagccgaat cctaatgtta caaaaggtgt
    59221 ctgtccatta gatgtatata aggtattaat ttcatattct aaactttcaa tcgcatcatt
    59281 gatgtcttta gtgacttgtt gatcaacata acgatcaatt tcagattctt tgacaaattg
    59341 ctttgcgata ttcctatgtt gttcttcatt atgtcgtgca tatgtactaa gtaattcgtc
    59401 aacgcggtca accgtacagc caccatattg actgctagaa acattggcta taatttgtac
    59461 aagctgcgct gatgcagttt gtattgattt tggtgaagtt acattcgcgt tgcctatttc
    59521 aaatccatta tgtagcatat ttttagcatc tattaaacaa cagttagtta acggttggaa
    59581 tggatgataa tctaagtcgt gaaaatgtat gtcccctctt tgatgtgcat ctgcaacgtg
    59641 ctttggtaat aaatgattta atgcgtaaga tttagaaacg atacctgctg ttaaatctct
    59701 cattgttgaa aatgtatcac tatctttgtt agcattttcg ttaacaatag caggatcttt
    59761 agaaatcaaa cccgttaatg cttcttcgat ttgattcatt ttaccccttc ttcctatata
    59821 ttgtgtctac tcattacgtt ataccactat atatagtatt ttcaaatttt tattagtctg
    59881 ttaactaaaa cgtcacaaag ttattaacat ttagtgaaat tcatatttct acacaattat
    59941 ttcattaatt tttaaaattg tttaaagatt cgtccgtcat ttcgacatgt ttatgcaact
    60001 attatttcat tttgcaacgt gaccatagtt tctatttttt catacttaaa tcgtcacaat
    60061 gcccatcaac attgcaatta ctaacataac gatagcgaat ccccaaatcc aaaagaatgc
    60121 atacttaata tacgtgccca tgtttgcctc tgctaaacca attgccaacc ataaggctgg
    60181 tgaaaatggg ctgacaaatg tacctataat attccctatg accattgaat aagctgttga
    60241 tacagacggt acaccaaatt gccctgctgt ttgttcgaca atcggtaaca cagcaaaata
    60301 ataagcgtct gtgctagtta gtaaatctaa tggtacgcca agtaaaccta caataatatg
    60361 caagtatggt cctacttctg caggaatcac tttgattaaa ttggtcgcaa tcgctttaag
    60421 cataccggtt tcatttagta cacctaaaaa catacctgct gcaataatca ctgcagccat
    60481 cattaatgca ttcggcgcat gcgctcttaa tcgttccatt tgttcatcca ctgatttaaa
    60541 attaataaca agtgccaacg aaacacctat catgaatgca aattcaggtg gcgcaatatt
    60601 tattaacatc gatagaataa cagctaaagt taaagctgta ttcacccatt ttatccatga
    60661 ttttgttctt gcacgtcctt ttacaggaaa ccttacatct tgatctcgtt catatacttc
    60721 aactaattta tgtacatcta tatcttgtgt ttgcggtaat tcatttctct ctattgcttt
    60781 tttgatacgt ttctgttctt taaatccaag atataccgca aacaacataa caagaatgaa
    60841 acctattatt tgaataggta ttaatccata ccataattca ttgacacttt tggcttttaa
    60901 cactgcagct acacgagcca ttggacctcc ccaaggtacc atgttcataa tcgccgcgct
    60961 taatgctaat agtaaaatca ataaatattt attcatattt aacgctttat ataaaggtaa
    61021 taatgcagga atagaaagca aaaatgttac cgcaccggcc ccatctaatt gggctattgt
    61081 gccaattaaa gctgtcattg cacagacaat gacgacattg cctcgtgtca ttaatattaa
    61141 gcgtttgaca agcggcttga ataaaccact atcgttcatg atgccaaaga aaataatggc
    61201 aaagataaac ataataacaa cgttgatgac ttgatctaac cctttagcaa aaaatccaac
    61261 caaatctgtc acactatatc ctaaaatcat tgcccctaag caaggtatga ttgtcatacc
    61321 tacaactgga tttatctttt tggcaatgag taaacctaca attgaaataa taataatgag
    61381 ccccattact gttaaccaca tattatcact attcatatta ttttctccct ccacttttaa
    61441 tacaatttac ataatccccc tctttaactt aaatattata attaatataa ttttttatat
    61501 ttaagttatc tcattttatc aaaaaaatct tttttgcgta actatcaata gtgaagtttg
    61561 attttcctac ttgataacat ggcatgacta atccttgtat atcctgtcct gtatttcatt
    61621 ttgatctatc atttttatta ttgctttcta ttgcacaaaa aatctggcta aaggtcgcta
    61681 tgatagtaac gacttcaacc agatttcaat ttatccacct attttattaa gtttgttcta
    61741 cgtatctttt tctctattgt aatattcata tttaaaacgc atatcattat cgcttaaatc
    61801 cttcattatt agactattca caaagccgcc catgtcttaa tttcaatgat taagcgttaa
    61861 agttaaaata agaagactta ccttagacgg ttagacggtc cgctattatt atcttacatc
    61921 caaccacgct aaaaattgag cttgtttgtc atgatttaaa tattcttgtg acacaatttg
    61981 tgacagtaac tgttgctttt cgttatatgt tatatcaagt acttttattt tctgtcggca
    62041 agtatataaa aagtcgatat acgaactgta tgatggtgga tataacgcct caagctctgc
    62101 cataattgat tttgtcaact taggactcgc accatcagtt gatacactga tagttagctt
    62161 gtcgcggtgt agtgcacttg gaaatacaac attgccattt gatgcatctc caacattatt
    62221 aaaaagggca tgctcaggta aggctttttt taccgcttca ttgacacgtg gctcattggt
    62281 tgctgcaata actagatacg cgtctacaat atcgcttggt tcaaattctt tttctttcca
    62341 tatgacgaca ccgttatcta ccatattttg aagtttttca gtgattgtcg gactgatgac
    62401 cgtcatatgt tcaacgtatt gatttaatgt ttgtgcccga cgacttgcaa cgacgcctcc
    62461 accaactatg acgacatttt tatttgttaa atctatcatt aatggcatat tcatgttgag
    62521 caacatcctt tatatctttt tacatcatta caccttaaaa tatttaacac caatcgctaa
    62581 taaacatcac gttgatagcg ttgttgttgt ttcatttgtc gcaataataa ctctgcttct
    62641 tcttgagaaa tatgacgttc ttttaccaat acatctttaa tggcttgatg gacatctttc
    62701 gccatacatt tttcatcgcc acaaatatag attgctgcgc cttgctcaat ccattcattg
    62761 aaacgtttac tttcttccat tatacgatgc tgtacatatt ctttgtgttc ttggtctctt
    62821 gaaaatgcta aatctacgcg tgttaagttt ccattttcaa gccattcttc tatttcttct
    62881 tcatataaaa agtcagaact acggtgttga tcaccaaaga acaaccatgt ttttccagtc
    62941 ataccaagtt cttcacgttc ttgtaaatat gctctaaaag gagcaattcc agtacctggt
    63001 ccaatcataa taaccggtat atcttgcttc atcggaaatt tgaagttcgg atttttcttc
    63061 aaataaattg gtactatatc gcctggttta attcgctcag caaaatgaac cgagcataca
    63121 ccttttctct cacgtccatg tgcttgataa cgaaccgtac caacggtaat atgcacttca
    63181 tctggcgttg ccataaaact actagaaatc gaatactctc ttggtggtaa ttttctaagg
    63241 atttgataca tattctcagg ttgtaattct atagttggaa aatctgttat taaatctata
    63301 aagtcccgat ttataacata ttcacgcgcc catgactcat cttgaatacg ttcagataat
    63361 tcttcattgt caaaatagat atctgcattt ttcaataatg gcaatgttaa tttagtaaat
    63421 tcaaaatgtg atgttagtgc ttcaacaata ggaactgtat caccatgatc attaattggc
    63481 accggagatt gcggatccca acctaacatg aatattagtt tttcaaccaa ttcagggtcg
    63541 ttttgcggta atgctactat acaatctcct ggttcatatg attcactaaa atcatcaagt
    63601 aaaaattcta tatgtcgtgt ttctttattt gaatcggtac catttaaatt gatattcgct
    63661 aatacttctg cttggtatgg atttgattta gaatatttct tttctttggc agacttaatt
    63721 gattcactta tcaccgattc actttgaata ccttctgatg tggtatcaat aatattaatg
    63781 atgtctgcca tccacttttc tgcgtcttct tcataatcaa tatcacaatc tacacgctta
    63841 catatacgct cagcgcctag attttctagt aaaacatcta catctttacc ggcttgacag
    63901 aaaaattcat atgtttgatc acctaaagct agtactgaat atctcacatg atttaaatta
    63961 ggtgcgttat cgtcttctaa aaattcaaag aaatcccatg cattatcagg cggttctcct
    64021 tcaccatgag tagacgtaat aataaataaa tcttctaact gcgcgatgtt tgtcgtatca
    64081 tattcatcca ttgacatcaa aacaacttga tgtccgatat cacttaaacg ttctgaaaaa
    64141 atttcagcta aacgcatggc attacctgat tcagaaccat atagcactgt aacatgccgc
    64201 tgattagctt cgatatgtgg ctctttattt tgtaacatat acgttgttga agttgcttgt
    64261 tcttctgata tttgtgattg ttgccacact tctgtctcta attgttgttg attactatca
    64321 cttgtcgtat cgttactatt agctagtaaa aaaccactaa gccactgttg ttggctctct
    64381 gttaatgttt gcagcagatt gttaatctct gtgacttgtt tttctgtaaa tggactatta
    64441 gatgtattta atttcaaatg ttttcacctc aataaacttg aaatagatga ttgactcttt
    64501 ttttatcgat agtttagtca attttttaat atgtatggta taaattaaaa caaacttgag
    64561 ttgtgtctga tagttatact tgcaaatatt tacaaccttc atgttgcttg aatgacatta
    64621 tcagtaattc agacaaaact ttgattatgt cgtgtcagtt attaatgcat acagtaatgc
    64681 atcgttgtta ttttttattt atttactatt tattatgtac caaatcagcc tttcagtaaa
    64741 ataggtcaaa ttactgattt tctaaatata aaatgcctcc taataacata ctattagtac
    64801 atcattaaga ggctcttgtg ttatttgcat actaagcgct caaatttaaa tttaagatga
    64861 agattcttgc aataatattt ctatatttgt cgatatatcc aatggatctt ccattggcaa
    64921 gaatcgattt acaacatttc cttgtcgatc gattataaat tttgtgaaat tccattttat
    64981 tggggaccca aagattcctg gttgttcatt ctttaaatgc gtaaataacg gatgttcatc
    65041 gttcccgttc acagatattt tagctaacac tggaaatgtt acaccaaatt tctcacgact
    65101 aattttcaag atttcttcat tagaacctgg ttgtcgattg tcaaaattat tattcggaaa
    65161 actcaacact acaaacccac gatccttata tttttgaaat aaagtctcta gttttttcaa
    65221 ttgttcgcta tatatacatt ctgttgcagt attaacaact aaaatcactt tacctctaaa
    65281 tgcttctaat ttataagtta agcctttata atcacttact tcgatatcat acacatttct
    65341 attattcata agacacccct acacagcctt ttttatattg aatatgttct ttttagaatg
    65401 ttctgataaa ataagtgcgc gtttacaccg tgaacacaca ttatatagcg tgatacattt
    65461 ttcgagcaca cgataaataa tgttcgagtt tatgttgttg ctcaacctat ccgatttacc
    65521 gtcttttttc acctgtctta ctttattaaa ataatcacat tgtatagctg tattagtcca
    65581 gctacaatat cttccgttat aacccctcat tataatcatc cttattttct atttttaaaa
    65641 agacaattag accgctcttt aaactataga ttaatactta agttaaactc atacatactg
    65701 ataccatacg ttagatctaa caatttaaaa ttcgttataa ctatggatta aagagctgcc
    65761 caactcatat aatccttaaa aacttcacat gtgattgttt attaagccct cctttatctt
    65821 attaaatatc cttataaccc ttttaaaatt aaactgacac actcatacat tgttacacgc
    65881 gacgataaac gtcatattat tgcatatcat taaaacaagt cactttgtat cgtgtgcgac
    65941 cgaatacata ttaaatcatc aataatgatt attcaattat taaaaataaa aacgaacatt
    66001 ctatatagag tttctactta ttattttttc agcattgtct acattactta acattcgtgt
    66061 ttgtaagtaa tattgaccgc caatatttag acactttata agtatgccat tcatcatttt
    66121 taatcatatt atttttctta ttgatatttc
//
LOCUS       DALAAR010000005        63731 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000005 DALAAR010000000
VERSION     DALAAR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 63731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.005"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(192..695)
                     /locus_tag="O2458_000311"
     CDS             complement(192..695)
                     /locus_tag="O2458_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ3886200.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(688..1275)
                     /locus_tag="O2458_000312"
     CDS             complement(688..1275)
                     /locus_tag="O2458_000312"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCZ3886201.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1287..2087)
                     /gene="racE"
                     /locus_tag="O2458_000313"
     CDS             complement(1287..2087)
                     /gene="racE"
                     /locus_tag="O2458_000313"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCZ3886202.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2323..3138)
                     /gene="sdhB"
                     /locus_tag="O2458_000314"
     CDS             complement(2323..3138)
                     /gene="sdhB"
                     /locus_tag="O2458_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCZ3886203.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3138..4904)
                     /gene="sdhA"
                     /locus_tag="O2458_000315"
     CDS             complement(3138..4904)
                     /gene="sdhA"
                     /locus_tag="O2458_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCZ3886204.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(4956..5570)
                     /locus_tag="O2458_000316"
     CDS             complement(4956..5570)
                     /locus_tag="O2458_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCZ3886205.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5894..7675)
                     /gene="uvrC"
                     /locus_tag="O2458_000317"
     CDS             complement(5894..7675)
                     /gene="uvrC"
                     /locus_tag="O2458_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCZ3886206.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7841..7936
                     /locus_tag="O2458_000318"
     CDS             7841..7936
                     /locus_tag="O2458_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886207.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(7999..8313)
                     /gene="trxA"
                     /locus_tag="O2458_000319"
     CDS             complement(7999..8313)
                     /gene="trxA"
                     /locus_tag="O2458_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCZ3886208.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8486..10834)
                     /locus_tag="O2458_000320"
     CDS             complement(8486..10834)
                     /locus_tag="O2458_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCZ3886209.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10844..12556)
                     /gene="polX"
                     /locus_tag="O2458_000321"
     CDS             complement(10844..12556)
                     /gene="polX"
                     /locus_tag="O2458_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCZ3886210.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12629..13150)
                     /locus_tag="O2458_000322"
     CDS             complement(12629..13150)
                     /locus_tag="O2458_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCZ3886211.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13151..13417)
                     /gene="zapA"
                     /locus_tag="O2458_000323"
     CDS             complement(13151..13417)
                     /gene="zapA"
                     /locus_tag="O2458_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCZ3886212.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13787..14725
                     /locus_tag="O2458_000324"
     CDS             13787..14725
                     /locus_tag="O2458_000324"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCZ3886213.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(14896..17298)
                     /locus_tag="O2458_000325"
     CDS             complement(14896..17298)
                     /locus_tag="O2458_000325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCZ3886214.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17298..18356)
                     /gene="pheS"
                     /locus_tag="O2458_000326"
     CDS             complement(17298..18356)
                     /gene="pheS"
                     /locus_tag="O2458_000326"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCZ3886215.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(18737..19477)
                     /locus_tag="O2458_000327"
     CDS             complement(18737..19477)
                     /locus_tag="O2458_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCZ3886216.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(19717..19824)
                     /locus_tag="O2458_000328"
     CDS             complement(19717..19824)
                     /locus_tag="O2458_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886217.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(19861..20184)
                     /gene="isdG"
                     /locus_tag="O2458_000329"
     CDS             complement(19861..20184)
                     /gene="isdG"
                     /locus_tag="O2458_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCZ3886218.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(20203..20937)
                     /gene="srtB"
                     /locus_tag="O2458_000330"
     CDS             complement(20203..20937)
                     /gene="srtB"
                     /locus_tag="O2458_000330"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCZ3886219.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(20999..21964)
                     /gene="isdF"
                     /locus_tag="O2458_000331"
     CDS             complement(20999..21964)
                     /gene="isdF"
                     /locus_tag="O2458_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCZ3886220.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(21977..22855)
                     /gene="isdE"
                     /locus_tag="O2458_000332"
     CDS             complement(21977..22855)
                     /gene="isdE"
                     /locus_tag="O2458_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCZ3886221.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(22852..23928)
                     /gene="isdD"
                     /locus_tag="O2458_000333"
     CDS             complement(22852..23928)
                     /gene="isdD"
                     /locus_tag="O2458_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCZ3886222.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(23928..24611)
                     /gene="isdC"
                     /locus_tag="O2458_000334"
     CDS             complement(23928..24611)
                     /gene="isdC"
                     /locus_tag="O2458_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCZ3886223.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            24820..25872
                     /gene="isdA"
                     /locus_tag="O2458_000335"
     CDS             24820..25872
                     /gene="isdA"
                     /locus_tag="O2458_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCZ3886224.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            26075..28012
                     /gene="isdB"
                     /locus_tag="O2458_000336"
     CDS             26075..28012
                     /gene="isdB"
                     /locus_tag="O2458_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCZ3886225.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(28166..28339)
                     /gene="rpmF"
                     /locus_tag="O2458_000337"
     CDS             complement(28166..28339)
                     /gene="rpmF"
                     /locus_tag="O2458_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCZ3886226.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(28419..28976)
                     /locus_tag="O2458_000338"
     CDS             complement(28419..28976)
                     /locus_tag="O2458_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCZ3886227.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            29103..30242
                     /locus_tag="O2458_000339"
     CDS             29103..30242
                     /locus_tag="O2458_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCZ3886228.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(30304..30786)
                     /gene="coaD"
                     /locus_tag="O2458_000340"
     CDS             complement(30304..30786)
                     /gene="coaD"
                     /locus_tag="O2458_000340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCZ3886229.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(30788..31330)
                     /gene="rsmD"
                     /locus_tag="O2458_000341"
     CDS             complement(30788..31330)
                     /gene="rsmD"
                     /locus_tag="O2458_000341"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCZ3886230.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            31400..31789
                     /locus_tag="O2458_000342"
     CDS             31400..31789
                     /locus_tag="O2458_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886231.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(31792..32046)
                     /locus_tag="O2458_000343"
     CDS             complement(31792..32046)
                     /locus_tag="O2458_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCZ3886232.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            32285..33211
                     /locus_tag="O2458_000344"
     CDS             32285..33211
                     /locus_tag="O2458_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ3886233.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(33274..33708)
                     /locus_tag="O2458_000345"
     CDS             complement(33274..33708)
                     /locus_tag="O2458_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCZ3886234.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(33724..34761)
                     /locus_tag="O2458_000346"
     CDS             complement(33724..34761)
                     /locus_tag="O2458_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ3886235.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(35088..35549)
                     /locus_tag="O2458_000347"
     CDS             complement(35088..35549)
                     /locus_tag="O2458_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCZ3886236.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(35574..36485)
                     /locus_tag="O2458_000348"
     CDS             complement(35574..36485)
                     /locus_tag="O2458_000348"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCZ3886237.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            36925..37836
                     /locus_tag="O2458_000349"
     CDS             36925..37836
                     /locus_tag="O2458_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCZ3886238.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(37976..41428)
                     /locus_tag="O2458_000350"
     CDS             complement(37976..41428)
                     /locus_tag="O2458_000350"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCZ3886239.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(41982..43208)
                     /gene="ftsW"
                     /locus_tag="O2458_000351"
     CDS             complement(41982..43208)
                     /gene="ftsW"
                     /locus_tag="O2458_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCZ3886240.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(43522..43797)
                     /locus_tag="O2458_000352"
     CDS             complement(43522..43797)
                     /locus_tag="O2458_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCZ3886241.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            43938..44420
                     /locus_tag="O2458_000353"
     CDS             43938..44420
                     /locus_tag="O2458_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886242.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            44422..44589
                     /locus_tag="O2458_000354"
     CDS             44422..44589
                     /locus_tag="O2458_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCZ3886243.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(44729..46576)
                     /gene="typA"
                     /locus_tag="O2458_000355"
     CDS             complement(44729..46576)
                     /gene="typA"
                     /locus_tag="O2458_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCZ3886244.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            46678..46869
                     /locus_tag="O2458_000356"
     CDS             46678..46869
                     /locus_tag="O2458_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCZ3886245.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(47023..47850)
                     /locus_tag="O2458_000357"
     CDS             complement(47023..47850)
                     /locus_tag="O2458_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ3886246.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            48007..48621
                     /locus_tag="O2458_000358"
     CDS             48007..48621
                     /locus_tag="O2458_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCZ3886247.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            48805..50157
                     /locus_tag="O2458_000359"
     CDS             48805..50157
                     /locus_tag="O2458_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCZ3886248.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(50239..50658)
                     /locus_tag="O2458_000360"
     CDS             complement(50239..50658)
                     /locus_tag="O2458_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ3886249.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(50941..51960)
                     /locus_tag="O2458_000361"
     CDS             complement(50941..51960)
                     /locus_tag="O2458_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ3886250.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(52034..53107)
                     /locus_tag="O2458_000362"
     CDS             complement(52034..53107)
                     /locus_tag="O2458_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ3886251.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(53107..53916)
                     /locus_tag="O2458_000363"
     CDS             complement(53107..53916)
                     /locus_tag="O2458_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3886252.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(53922..54719)
                     /locus_tag="O2458_000364"
     CDS             complement(53922..54719)
                     /locus_tag="O2458_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3886253.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(54712..55806)
                     /locus_tag="O2458_000365"
     CDS             complement(54712..55806)
                     /locus_tag="O2458_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886254.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(55819..56358)
                     /locus_tag="O2458_000366"
     CDS             complement(55819..56358)
                     /locus_tag="O2458_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3886255.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(56502..56777)
                     /locus_tag="O2458_000367"
     CDS             complement(56502..56777)
                     /locus_tag="O2458_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCZ3886256.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(56945..58351)
                     /gene="lpdA"
                     /locus_tag="O2458_000368"
     CDS             complement(56945..58351)
                     /gene="lpdA"
                     /locus_tag="O2458_000368"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ3886257.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(58355..59647)
                     /locus_tag="O2458_000369"
     CDS             complement(58355..59647)
                     /locus_tag="O2458_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ3886258.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(59738..60715)
                     /locus_tag="O2458_000370"
     CDS             complement(59738..60715)
                     /locus_tag="O2458_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ3886259.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(60719..61831)
                     /gene="pdhA"
                     /locus_tag="O2458_000371"
     CDS             complement(60719..61831)
                     /gene="pdhA"
                     /locus_tag="O2458_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCZ3886260.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(62002..62628)
                     /locus_tag="O2458_000372"
     CDS             complement(62002..62628)
                     /locus_tag="O2458_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCZ3886261.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            62993..63544
                     /locus_tag="O2458_000373"
     CDS             62993..63544
                     /locus_tag="O2458_000373"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ3886262.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT        20629 a        11730 c         8949 g        22423 t
ORIGIN      
        1 caacatgatt caaccatata gggttatcaa caatcacatt ctccaaaatt acaattcttt
       61 tcataacata aatgtgttat tcattccaat tatcttataa aattgtcaga aatactttta
      121 aacaaacatt catgacacga cctcctagtt aaatcagtct ataaactaaa aaaagccata
      181 gctacttcta tttaaaaagt agtatgactg attgtttcgt gatgtcgatt tttgaaattg
      241 atggtgccat gtaaagtttg atcatcaata ataacttcag catatgtttc ttccattgaa
      301 cttctagatt gagatatact tccaggatta ataacatgaa caccattaat atactcatat
      361 tttgccacat gtgtatgtcc ataaaatgca aacaaacaac ctaattcaag tcctttttca
      421 gctaataaat ctcttgttcg attgacttga tataaatgtc catgagtata aaatgctttc
      481 acgtcattat attttgcgac cgcttcattt tcaaattctg ggtaaaaatc acaattgcct
      541 tttactctat taaataagct aagttccgta tcatcatacg cgaattctga atctcctaaa
      601 tgtaaatata catctgcatc tgggtgcatt tcataaattt gatataaaac gcctgattca
      661 gtatggttat cactcacaat aatccattta gacattttta tcaccttcaa gaaaagcttg
      721 aagtaaatta atcgcatttc gtctatgact aatttgccct ttttgttctt ttgaaagttg
      781 agccatggtt ttatctaatt tcggtacata aaatatcgga tcatatccga aaccattttc
      841 gccatatttt ccatctgcaa tttcacctga aacagtacct ttaaatactt ttgtttccat
      901 atcagggcca ctcatactta tgacacaaac aaattgcgca cgacgatcag ttgtattacc
      961 aagcttattt aataattttt caatatttgc ttcatcgctt ttattttcac cagcataacg
     1021 tgcagagtat atacctggct caccatttaa tgcaaaaact tctagtccac tgtcatcagc
     1081 tatgaccgtt ttattcaatg cttttgcagc agcttctgat tttaatatag cattttcttc
     1141 aaatgttgat cctgtttctt ccacatcaaa atctggtatt agttctgaaa tacctattac
     1201 gtggtaatct ggaaatatta ctttaaagtc atttattttc ccttgattat tcgatgcaat
     1261 aacaatctct ttcattaaaa atcctcctag tcattcactg atatacgttc cacattgaca
     1321 gataaattta accactcttt gataatgtta gtaatgtgag taggatcacc tgttgcaaaa
     1381 aatcgatgat ctggatgttc agtataactt gcatgttcat tactaaatgt tagcaatgca
     1441 ctaacttcac gagccgtttc taatccagac gaaatcactg tctttttacc accaaaataa
     1501 tcatagatag gtttatagag caatggatag tgggtacatc ctaaaatgac agtatcagac
     1561 tcactattac gccaacgttt cagtgtttga tgaatgacaa tgcttgtaat tgttggatca
     1621 ctatatctca tttgttctac aagtggcaca aaacctggac aggcaacgcc atgtacctct
     1681 acatgtggat tgatacgttt aatatgcgta cgatatgctt cagatttaat tgtgccttcc
     1741 gttcctagta ctaatacatt ttgatttcta gtagtcatta ttgctgttct agcacctggt
     1801 tcaattacgc caatcactgg gattgataag gtcttttgta aatattctaa agctacagca
     1861 gttgctgtat tacaagcaat cacgagcatt tttatatcaa attccattaa tttacgagcg
     1921 atttcaactg tatattgttt tacttgttct cctggtcttg gcccatatgg acatcgtcca
     1981 atatcaccta agtaataaat cgtctcattt ggcaactgac gcataatttc tttagctact
     2041 gtcaaacctc cgacaccaga gtctattaca cctattggtt tattcatatt cggtcatcct
     2101 tacttttaat ttaattttta gtttatcata actaagcatt ggattttagt attatgcact
     2161 gtgtttacca tttttgtcat tataatattt attttaaatc agcccactat catattgtca
     2221 tgtaatctgc ttattaaaaa aatcccttcc aagttattgt gtatctccat tcaatttaat
     2281 tttgaaagga acataacatt ttaactcaaa agggattaat ttttattcta cttcatggtc
     2341 tgaaccaaag aatgatttaa acatgtggaa tgttgtttct ctgttcattg ctgcaatgga
     2401 tgttgttaat ggaatacctt tagggcaagc attaacacag ttttgtgaat taccacactg
     2461 ctgtaagcca ccagtaccca ttaatgcatt taaacgttca tctttagtca tagatcctgt
     2521 tgggtgcaaa ttaaacaaac gaacttgcga gattgcttgt gcaccaacga atttattatt
     2581 ttcagtaaca ttaggacaaa cctctaaaca tacaccacat gtcatacatt tagataattc
     2641 ataagctgtt tgacgttttt tctctggcat acgtggtccc ggacctaaat catacgttcc
     2701 atcaattggg atccatgctt tcatacgttt taagttatcg aacattctag aacgatcaac
     2761 ttgtaagtca cggataactg ggaaagtatt cattggctct aaacgaatag gttgttctaa
     2821 ttgatcaaca atcgcagaac aagattgtct tgcacgacca ttgataacca tagaacatgc
     2881 tccacatact tcttctaagc agttcatatc ccagacaaca ggtgttgttt tttcaccttt
     2941 aatattaact gggttacgtc taatttccat taaacaagca atgacgttta aattttcacg
     3001 atatggaatt tcaaatgttt cttcataagg cttagaatca cttgtatctt gtcgtttaat
     3061 aattaatttt actgtttttt gtttcggttt agattgtgtt tcatgttgtg gagtgttttt
     3121 cactgattgt tcagtcatta ttttttaccc cctttagact tacttgtgta atcacgttta
     3181 cgaggtggta ttaaactcac atcgacgtca tcataagtaa actgcggttt ttcaaatgcg
     3241 ccttggaatg aggccattgt cgtttttaac cactcttcat cattacgctc tgggaattct
     3301 ggtttataat gggcaccgcg tgattcgtta cggttatatg caccaatcgt aataacacgt
     3361 gcaagtacta acatgttcca tagttgacgg gtaaagaata ccgcttggtt actccaagtt
     3421 tgagtatctt ccatatcaat atcttcataa cgtttcatca attcaacaat ctttttatct
     3481 gtttctaaca gtttttcatt ttcacgaaca acagttacat ttgctgtcat aatttcacca
     3541 agttcacggt gtaatttata tgcattttct gtaccgcgca tagctaataa tttatcaaaa
     3601 cgttcttgct cttcagcttt acgcttttca aaaatacttt cgtccatatc agtatatgat
     3661 cgatcaatat ttgaaatata atcaatcgcg tttggacctg ctactgtacc accataaatc
     3721 gctgataaca atgaattggc acctaagcgg ttaccaccat gttgagagaa gtcacattct
     3781 ccagctgcaa ataacccttt aatatttgtc atttgatcat aatctacata tagaccaccc
     3841 attgaatagt gaacagctgg gaaaatcttc attggtactt tgcgtgggtc atcaccagtg
     3901 aatttttcat aaatctcaat gataccacct agttttacat ctaactcatg tggatcttta
     3961 tgtgacaaat caagatatac catgttttcg ccatttatac ctaatttttg gttaatacat
     4021 acatcgaaaa tttcacgcgt tgcgatatca cgaggtacta agttaccata atcaggatat
     4081 ttctcttcta agaagtacca aggcttacca tctttatatg tccaaattcg tccaccttca
     4141 ccacgtgctg attcactcat tagtcgcagt ttatcatcac cagggattgc agtaggatga
     4201 atttgaatga actcaccatt agcataaata gcgccttgtt ggtaaacaat ggaagccgct
     4261 gatcctgtat taatcattga gtttgttgtt ttaccgaaaa taataccagg gccacccgtt
     4321 gccataataa ctgcatctga accaaatgtt tcaatctcag cagttgtcat attttgtgca
     4381 acgatacctc ttgcactatc atcgtcacct ttaactatgc caaggaattc ccatccttca
     4441 tacttcgtaa ctaatccatc tacttcatat gcacgaactt gttcatccaa tgcatataat
     4501 aattgttgtc cagttgttgc ccctgcatat gctgttctgt gatgtaatgt accaccgaaa
     4561 cgtctaaaat ctaatagacc ttcatttgtt ctattgaaca ttacgcccat acggtctaat
     4621 aaatgaataa ttttaggtgc tgcctctgtc atcgctttaa caggtggttg gtttgcaagg
     4681 aaatcgccac catacactgt atcatcaaag tgaatccaag gagaatcgcc ttccccttta
     4741 gtattgaccg caccattaat gccaccttgg gcacaaacag agtgcgaacg ctttactggt
     4801 acaactgaga acaaatctac atgtgcacct ttttctgccg ctttaattgt tgacattaag
     4861 cccgctaggc cacctccgac aacaataaga tgtttctctg ccataaaaat ttcactcccc
     4921 taaattttca atctatattt gttaaatgcg atgtattaca taaaggcaat aattgcagta
     4981 acaccaatat acgaaataac taaaaatacg attaatgaaa cccatgtaaa tactcgttgt
     5041 gattttggag attgaagtcc accccaagta actaagaatg accataagcc atttgcaaag
     5101 tggaacacaa cagcaataat acaaataata taaaatattg cccatccagg atgttgcaat
     5161 gtttcgtgca ttaaatcgta attcacttct ttgccgtaaa atgctttttg taaacgtgtt
     5221 tgccataaat ggataccaat aaagataaat gttaagatac cactcactct ttggaagaag
     5281 aacatccagt ttctaaaaat cgagtaatgt ccaacatttt cttttgctgt aaatgcaatg
     5341 tgtataccaa acaaaccgtg atataacaac ggaatgtata taaataaaaa ttctacaata
     5401 attagaaatg gtaatgattc cataaagtta gatgccttat taaacgcttc agcaccttgt
     5461 gttgcttggt gattcactaa taaatgaacg accaaaaatg cacctattgg gataatacct
     5521 aataacgagt gaatacgtct tagataaaat tcattttttg attgagccaa aaggagtccc
     5581 ccctgtgaac gaatatttaa tttattgagc tatttatatt aaacgtacgc ttaaccccct
     5641 aaagtgatat cgtttctagc gttgtcatta tcattaagcg aaacatttta aagacaaata
     5701 cacactgcac gatcaccaaa ctgcatgtcg aacaatgtaa catttggatt cgatatttaa
     5761 aattgcttgt gatgataaac tttctcattt agaaaacgct tccacgtaca ttcaaaaaaa
     5821 taactttgtt aaccatattg taacattatt tcatatattt tggggcatga gaatgattct
     5881 cacgcccagt aatttattta tgcaattgtt catgtaggtt ctttgcgacg ttttcaggaa
     5941 tacctatatt tttaaaatct tcaagtgtag cttccttcat tttcttgatt gaaccgaatg
     6001 aacgcaataa taatgtttta cgtttgttac cgataccatc tatatcatca agtattgatt
     6061 tcaagcctgt cttttgacgt gtttgtctat gaaatgtgat tgcgaatctg tgaacctcat
     6121 cttggatacg gtgcaacaaa taaaatgcct ggctattttt cttcagtggt acaatttctg
     6181 cactagcgcc atataataat tcagatgttt ggtgtttatc atttttctgc aaacctgcaa
     6241 cagggatatc aagacctaat tcgttttgta gcacatcaat aaccccgttc atatgtcctt
     6301 taccaccatc tactattatt aaatcaggta atggtaatcc ttcgtttaaa acgcgagaat
     6361 atcgtcgtct tactacttct ctcattgatt tgtaatcatc tggaccttta accgttttga
     6421 ttttatactt tctataattt ttcttatctg gtttaccgtc gacaaatgta accattgctg
     6481 acactggatc cacaccttga atattagaat tatcgaatgc ttcaattcta attggtgttt
     6541 gaattcccat ttgtgttcca agttcttcaa tagctttaat cgttctggac tcatcacgtg
     6601 atattaattc aaatttatta tttaaggata ctttagcgtt atgtgcagct aggtcaacca
     6661 tatctttttt gggacctcgc gcgggttgaa cgattttagt gtccacaaca gattgaatca
     6721 tttctttatc caaattacgt ggtacatgaa cttccttagg taaaatatgt tggtttaagc
     6781 tataaaattg tccaataaat gtataaaatt cttcttcttc tgtttgctgt aatggaatca
     6841 tcgttgtatc tcgctttatc atattacctt gtcgtataaa gaaaacttgg atacacatcc
     6901 atcctttatc aacactataa ccaaagacat cacgaatcgt tttatctgat gacataattt
     6961 tttgtttgtt tgtcagattt tgaatatgtt gaattaaatc tctatattct ttagcccgtt
     7021 caaaatcaag tgattcactt gcagttaaca ttcgctcttc taaacttttt aaaattgttt
     7081 tgtcttcccc attcagaaaa tcagtaattt ccttcgtcat ttgtgcgtat ttactcaaat
     7141 caacgtcata tacacatggt cctaaacatt gtccaatatg gtaataaaga cataatttat
     7201 ctggcatctt atcacatttg cgatatggat atattctgtc taataacttt ttagtttctt
     7261 gagcagaata tgcattcgga tacggtccga aatatttgcc agtaccttgt tttacagttc
     7321 tcgtcactag tagtctagga tatttctcct tcgtaatttt aataaatgga taacttttat
     7381 catcctttaa taatatatta tatcttggtt gatattgttt aatcagattc aattccagta
     7441 aaagtgattc tgtttcactt gacgtgacaa taaactcaaa gcgacgtatt tcaccaacca
     7501 gtctcgttgt tttagcatca tgagcacccg taaaatatga tcgcaatcga tttcttagct
     7561 ttttagcttt gccaacatat atcacttgat cattacgatc tttcattaaa tagcatcctg
     7621 gttccatagg tacgacattt aatttatttt taattcgttg cttatagtct tccaaaaata
     7681 tccccccttc taattttaaa atgaatgatg ctacaaaagt gcttcaaatt tatcaccact
     7741 aattctagtt aataatcaac atcttcgagt atgacttttc atgcacatca tgtaatattg
     7801 aacatggttt tatcaaacca actgctacaa atctatcagc atgctagatt atcatttaac
     7861 gttcatagat ttattaatcc caccattctc aagtcgtaaa caatatgatg acttatcatt
     7921 caacttttta tattaaaaac acaaacaaaa actggagcaa ttcattaaag aaatgcccca
     7981 gtcgtcattg gttgtaactt ataaatgttt atctaaaact tcagctaagt tttcttttgg
     8041 ttggaaacca acaactttat caactggttg accgtcttta aagacgatta atgttggaat
     8101 actcatcact tcatatttag ctgcagttga tggattttca tcaacatcta attttaaaat
     8161 gtcagcttta ccttcatagt cagctgctaa ttcttctaat accggagcga tcattttaca
     8221 tggaccacac catgttgccc aaaaatctac tagttgtaca ccagattcta cttttgaatc
     8281 aaaatctgca tctgttactt ttacgattgc cataaatgcc aatcctcctt aattattatt
     8341 atgatgttca aattatatca gataacatgc ttcatgtatt tctatttgct caatttttat
     8401 gcttatttca aataacatgt ttgaaatgta ttcttgttgc tttacattat tttaactgca
     8461 gctatttaat ttatcaaatt ataatttatt ttagtgttgc aacggtaacg ccaaatccac
     8521 cttcgcttgg cataccacct ctaaagtcac taacactttt atgctttttc aaatgttgtt
     8581 gtacaccttt ttgaagtgca cctgtacctt taccatgaat gatataaact tgttcgtaat
     8641 tacttaaaac ggcttgatct aaatattgat ctagttcaat taaagcatcc tcataacgat
     8701 agcctcgtaa gtcaagttca gttttaattg tttgacgatt ttgacgtgta accattttcg
     8761 ttggcttaac tttttctttt tgttttttct ctaaatcttc aataggtaac ttcattttaa
     8821 taattcccat ttgaacaatt gcttcttcat cattgacaat ttctaaaact tcaccctttt
     8881 gaccgtaaga taatactttt acttcatcac cagcaacaat tttatcgtat ttttgctttt
     8941 gtacattttg ctttatagat ttcgcttcat aatgatcatc taatcgtttc ttcttatcaa
     9001 tcaattcatg ttctttaaca tctgcacctt tttgttcacg caattgtctt aagtctttaa
     9061 taatatcgtc agcttctttt gttgcagcct taatcttctg atttgctttt tctttcgctt
     9121 cctctattag agacttttca taattttgga attgttgata ctgcttagat aaatcatcat
     9181 gcacttgctc cgcttctttt acaagacggt ccagttctaa cctctgtgtc tctacacgtt
     9241 tgtaattacg ctctaatgat tcaatcattt catttatttc tttttcatca gtaccaatca
     9301 tcgtcttagc cttattaata atattcaaac taagacctaa ctttttagaa atgtcaaaag
     9361 catttgaacg acccggcaca cccattaata acttatacgt tggactcaaa gtatctacat
     9421 caaattctac actcgcattc ataacgcctt ctcgattata actatatgct ttaagttcag
     9481 gatagtgcgt cgttgccatt actagagaac caatttttct aacatgatct aaaatgctca
     9541 ttgctaatgc agcaccttca ctcggatctg tacctgcacc taattcatca aataaaacta
     9601 aactatgttt gtctgcatgc tttaaaattt caactatatt cgtcatatga gatgaaaaag
     9661 ttgataatga ttgttctatt gattgttcat ctccgatatc gcaatataca tttttaaata
     9721 cacttaactg actaccatca agtgtgggaa tcaacaatcc tgattgagcc ataacaataa
     9781 ttaaacctaa tgtttttaat gttacagttt taccacctgt attcggtcct gtaataatta
     9841 ccgtttcaat atcttccata aattcgatgg tattagctac aacagtctca cgatttaata
     9901 atggatggta tgctttaggt aaatatacag tacggtcctc tttaaatatc ggctttgttc
     9961 ctttaatact tctactatat ctcgcttttg cgattaaaaa atctaactga cccatgactt
    10021 gttctgccac aagtagtgca tctttgtccg cagccacata accagttagt tgcgttaaaa
    10081 tgcgttcttt ttcaattgct tcgtcatgac gtaatcgact aatttgatta ttcatttcaa
    10141 caactgatga tggctcaata tacaatgttt gtcctgaagc agattgatca tgtacaatcc
    10201 cattaaaatc ttgtcgatat tcagctttga caggtataac gtttctttca ttcctaactg
    10261 ttacaatagc atctgataat tttttctgat ttgcttggct tttaacaata cggtccaaat
    10321 tttgtctaat acgttgattc gtgctagaaa ttttacttct aatcccttgc aattcataac
    10381 tcgcattatc atataaatca tacgtatcgc atgtttcatt tatttgttga aaaagatcag
    10441 ttaacacagg taattgattc atcttgtcat ctaatattgg gtatttaaca ccttcatctt
    10501 cttcaaccaa ttgattataa aatgtcttga attgattttg tacttgaatt aatcttttta
    10561 tcaagttaag ctctgataca tttaaaacgc cgccaatatc agcgcgatga atgaatgctg
    10621 atactttaga taagccactc aagcttggta aacgatgctt attatagatt tgagcaatct
    10681 catccgtttc ttccatttga aaaacaaccg tttcaaaatt agtagctggc atcatttgat
    10741 tgaccttttc caagcctaag tcactaatag tttcattggc aacgagtgat tttatttttt
    10801 caaattctaa gacgtctaat gttttttgtc tcataaaatc cctctatttc ttaagttttg
    10861 tattattttc aataaagtct ttaaaagctt cacgcgataa tgcgttaatc actctatctt
    10921 ttgttacaaa tcctttttgc gcagttgcta cgccataatt cataaaatct aaatgatttg
    10981 tatgatgcgc atcagtgtta atagttaatt tcacatttgg atatttacga acgatatcag
    11041 cgctcagatc cagtcgatgt ggattggcat taatttctaa tactgtattc gtttcttcag
    11101 ctaatgccat taattgttca atattcggtt tataaccatc tcttctacct ataatacgcc
    11161 ctgttggatg cgctatatgt cgcacgtatg gattgcgaca tgcattagct aatcgttcca
    11221 taatttgttc ttctgattgg ttaaagcttt gatgaatagc tccaattaca taatcaagtt
    11281 gtgctaaaat ttcatcatca taatccagcg agccatcagg taatatatcc atttctgtac
    11341 ctgaataaat atcaatttca ctatattctt tatctaaagc cttaatttct tcgttttgtc
    11401 ttaaaagtct ttctacttgt aagccattag caacacgtaa actttgtgaa tgatcagtaa
    11461 ttaccatgaa tttataacct tttgcgatat ttgcttctac catgtctcga atagaaaacg
    11521 caccatcact atacgttgta tgcatatgaa tatcaccatt aatatcatct attgtaatga
    11581 tattacttag atctttatca aattcgctac catcttctcg catagcaggt ggtataaaat
    11641 tcacattaaa atgttcatat atcttggctt cactatcata ttgaattaat gtaccatcag
    11701 cttgttcaat tccatattca cttacttttt catcacgtgc tttagcaagt tgtcgaattc
    11761 ttatattatg ttcttttgac ccagtaaaat gctgcaatgt atgataaaaa gcacttggtt
    11821 caattaatcg aaaatcgaca ccaatcgttt catcatcata cgctaattct aatgaaactt
    11881 ttgtgttccc cactgcaact tcttttactt tattgggaat atttaataat tgctgctgca
    11941 ctgcttttgg gttatcggta cttattatga aatctaaatc tttgctcatt tctttaaaac
    12001 gacggaagct tcctgcagat gaatattgat cgatataatt taatgtatct atataatcaa
    12061 tgatttcttg attaagtctt ctcatttgat caattggata tctatctttc ttagcaccaa
    12121 gttgtttcac agcttctaat atgttttgtt ccgttttctt agcaaatccg cttaattcac
    12181 taacttttcc attttcacaa gcaacttgaa gtgacgcttt atcaacaata ttcaactctt
    12241 tatatagctt agcaattttc ttgcttccaa gtccttgaat tttcaaaagt ggaataagac
    12301 cttccggaac ttcttcctgt aattgctgta aatactgaga ttcaccggtc tcacggtaat
    12361 cattgattac ttctgcaaca cctttaccaa tgccttttaa ctccgttaca tcagatattt
    12421 catctaatgg tcgttcatct aattcaagac tttgagctgc ttttcgatac gctgatattt
    12481 taaaagtatt ttcccctttt aattccatat aagtagcaat ttgttctaat agtttgataa
    12541 catctttttt tgtcataata acactccata aaaagaagac caggacgtat cattaatata
    12601 tacctttgtc ctgacctctt atgataattt ataaattaat ggtgaaagcc gataaatatg
    12661 ggatgtgaaa aatcaatgaa tgactcacaa tagatatttt aagctgatgt tgtaatgctt
    12721 cgttaggata taatgccaat agatataacg taaagtgtaa gacaattatc gtcataaaca
    12781 caccaactat cattcccatt gcacgactga aaatatgaat gttttgatac gctattattt
    12841 tatcaaaagt tacgataatt agatataaaa tgaacttaca aaacaatgta atcattaaaa
    12901 aagctacaat cgcttcaaat cgattttgta gatgattaaa atgaaacgca aaagttgtat
    12961 taaatgctgt tgttttagga tatggaataa atacaattaa tctttctaca atagatttgt
    13021 aaaattgact ggcaatccac aatgatacaa tcgttgcact caaatgtatc atagataacc
    13081 aaaaacctcg tctgaatcca acgatgacaa aatacacaaa gaaaatgatt atgataaaat
    13141 caatgaccat ttattgctca cgctgctgca atttgtgaat ttgttgtttc aaacgtcgat
    13201 tttcttcttc tagtagtact ttttcatgca taatattcac agcagttagt attgcttttc
    13261 ttgaagtatc taaacctgct gctttatacc ctaattcttt tattttatca tcaactaaat
    13321 gtgctacata tcgtatgtgc tctgggttat cttccccaac aattgtaaaa agctgatcat
    13381 taattgatac atttaccttg tttttaaact gtgtcattta taatttctcc tgatcctttt
    13441 tttaaaatct aaattcacgt tataaaatat gactggatag tttgtctgaa tttgatacta
    13501 atattgttat attgcaatta tgataaaaca acaacacaat ctctatagat gacttaatgt
    13561 tctttttata atgaaataat gtaaagaatt ttctattcaa tactttatca tgtttaaatt
    13621 gtgtcactat aacattttca taaacattat acatgaccac tatgtatttt gtaagtatcc
    13681 gcaattaatt ctttacaaca tacataaatg tttctgacgt tattatcatt tatgatatga
    13741 ttatttttgc taaagacaat gaaattttat gaaaggattt acacaaatgg cgaatatcgt
    13801 ttttaaattg tcggataaag acataacgac attaatgtca cgcatttctt ttgatactga
    13861 gaatttacct caaggtatga aagcacgtgc aaagtatcaa aatacaactg taaatattta
    13921 ccaatcaggt aaagtcatgt ttcaaggtaa tcatgcagaa gctgtgtctg aagaacttct
    13981 accgcagcat tctcaattga atactaataa aactaaaaag aaaaatatgg ctaattcttt
    14041 tcttgaacaa actttaatgt atgatcaatt taactgtatt ggtagtgatg aggctggaag
    14101 tggcgattac tttggtccgt tgactgtgtg tgctgctttc gttaccaaag agcatgtccc
    14161 tattttaaaa actttgggtg tagatgattc aaaaaaatta accgatacca aaattgtaga
    14221 acttgctgag caattggtta ctttcatacc acattcatta ttaacactac acaatgaaaa
    14281 atataatatt cagcaagcaa aaggctggac tcaagtaaaa atgaaagctg cccttcataa
    14341 tgaagccatc aaaaatgtac ttgaaaaaat tgattcgtct caattagact atatcgtcat
    14401 tgatcagttc gcaaaacgcg aagtgtatag ccattacgca ttatcagata taccacttcc
    14461 aaagaaaaca aaatttgaaa caaaaggaga atcgaagtca ttagccattg cagtggcaag
    14521 cataatttct cgatacgcat ttgttacata tatggatcaa atatcaaaaa atattaatat
    14581 gacgattcct aaaggtgccg gtgctaaagt cgatgtaatc gccgccaaaa ttataaaaaa
    14641 atacggtctt tcacgtttag ataccatttc aaaaaagcat tttaaaaatc gtgaaaaagc
    14701 ccaaaaaatt ttaaagcctc tttgattttt aatcattaga ggcttttatg cagttgtgcg
    14761 aagcatcatc tgtattccgc tgttcctctt tgacttttta aatgcattgg aaccaaattg
    14821 gtgaacccaa aaataaaacc ccatgttagg gatatcaatc tacttcagat atccttatac
    14881 atggggttta aatcattatc taataacagc accttgttca attaatgctg cttcaatttc
    14941 cgcttgtact tttgaaacgc gctcatctgt caatgtttct tctgtgtcta aataatttaa
    15001 acgtattgca attgattttt tacctttttc taaatgttcg ccctgatata catcaaagac
    15061 aagtgtatct tttaatatat tgccaccatg tgcatgaatc gttgataata aatcagctgc
    15121 tggaatattt tgatctactt ctaatgcaat gtcacgagac atgcctggga atctcggaat
    15181 tggctggtaa ttaatgtaac ctaccgacac agccattaat gcatcaaaat tcaactcaaa
    15241 aacatacgta cgttttaaat cattatcagc tgctaatatt ggatgtaatt caccaataaa
    15301 accaacaact ttattctcta ataagatttc agcagtacga cctggatgta atccatcaat
    15361 atcagcacgg cgataactaa attcaagatt taacttttca gatactcgat ccacgacacc
    15421 ttttgctaaa tagaaatcaa ccgtttcttt cttatcttgc cattgattga ctacataatc
    15481 tccagttaaa ataccactta aatattcaac ttgatctggt agttcacctt ctccattagc
    15541 aaagaagaca ttgccgattt caaataattt tacatcttta tttttgcgtg ccacattata
    15601 tgatgccgct tcgattaaat gtggtaataa actttgacgt aatgacgcat gcgcttcact
    15661 cattggcatc aataaatcaa ttgtttgacg ctgttgcatc gaaaatgcag tagcatcttc
    15721 tttagaaact aacgaatacg taatagcttg gtctaatcca gcaccttcta acacttcttt
    15781 aaccattcta gttttatatt ggcgatcagt tagctgacca ctagtaactt tatcgaagac
    15841 aggtaacgtt gatggaatat cgtcgtatcc atatatacgt gcaacttctt caattaaatc
    15901 ttctttaatt gtaatatctt tacgacgtga tggtactagc actgtaataa catcatcatt
    15961 tatttctgta tcaaacccta gttgattaaa aatagtaaca atatcatttt gtgacaaatc
    16021 aaatccaata gtgcgattaa ttttatcagc agtgatgtcg attggtgtaa taaatgcacc
    16081 aagttctcct gaagacactc tatcttttag cacttttccg tttgcataag tttgtaataa
    16141 ataacatgca cggtcgactg cttcatctac aaattcagta gctattcctt tttcaaaacg
    16201 actagatgat tcactgcgta aatttaaacg tcttgatgta tgacgaattg aaactggatc
    16261 aaaaatagca ccttcaatca ctatatttga tgtttgttct ttaacttctg aaaaatcgcc
    16321 acccataaca ccagctaatg caattggagt ttgtccatta gtaatgacaa tatcgctcgt
    16381 taataattca cgttctgtat catctaatgt tgtcattttt tcgccttcat tagcttgacg
    16441 aacaacaatt tgttgtgaac caatcgcatc ttgatcaaac atgtgcaatg gttgaccgta
    16501 ttctaataac acataatttg aaatgtcaac aacattatta ataggacgta tacccgcttt
    16561 tattaagcgt gcttgcatcc aaattggcga gggttcaatt gtcacgtcgt gaacaacacg
    16621 tgcactataa tatggtactt tatcttcatt ttcaattgtc acagtcagtt catcatttgc
    16681 agataaatca agctcatttg atgttgtctc tggcttagtc atttttgtat tatataatgc
    16741 tgcaacttca taagcagtac ctatcatact taaagcatct gcacgattcg gcgttaaatc
    16801 aaattccatt acttgatcat ctaaatataa agcttgtaag gcatctgttc ctggaacttg
    16861 ggcttcacta aatacataaa tgcctgattc aaaacttttc ggtatatagt tacttgaaat
    16921 accaatttct tgtaacgaac aaatcatacc ttctgaacgt tcaccgcgta atttggcacg
    16981 cttaatttta ataccaccag gcaatctgcc acctacttta gcaacaatga catattgtcc
    17041 tgcatcaacg ttcggtgcac cacaaacgat ttgtacaggt tcgtcttctc cgatatcaac
    17101 ttggcaaaca tttaatttat cagcatcagg atgtttctct tttgacttaa cgaagccgac
    17161 aactaaattt ttgatatctt ttgtgtagtc aattaaatca tccacttcaa tacctgtgcg
    17221 cgtaatacgt tctgccaaat tacttacaga atcatcgatt gttacatatt ctttcaacca
    17281 ttcatttgat atcaacatta catgtcacct ctatcttcta ccgctttaaa ttgatctaaa
    17341 aatctcacat cattagtata gaaatgacga atatcttcta taccatattt caacattgca
    17401 atacggtctg gtcccatacc aaatgcaaat ccagagtact ctgaagaatc aaaaccagcc
    17461 atttctaata cattaggatg taccattcca gcacctaaaa tttcaatcca tcctgtgtgt
    17521 ttacacacat tacaaccttt tcctttacat ttaaaacatg acacatcaac ttctacagaa
    17581 ggttcagtga atgggaagta acttggacgt aaacgaattt cacgatcagc accaaataat
    17641 ttcttagcta acaattctaa agtacctttc aaatcactca ttttaacgtt tttatcaaca
    17701 actaatcctt cgatttgtgt aaattgatga ctatgtgtcg catcatcaga gtcacgacga
    17761 tacactttac cagggcaaat aattttaact ggaccttgac catgacgtga ttccatcgta
    17821 cgtgcctgca ctggtgatgt atgcgtacgt aataaaattt catccgtaat atagaaacta
    17881 tcttgcatat cacgtgcagg gtgtgattta ggtaaattca gcatttcgaa gttataatga
    17941 tcttgttcaa cttcatatcc attcacaatt tcataaccta aacctaagaa taagtcttca
    18001 atttcttcta ttgtacgtgt taatggatgc tttgaaccga tttcaatatg acgacctggt
    18061 aatgatacat caattgtttc ttcagccaat tgcttattta atttttcttt aactaacatc
    18121 tgttgtcttt catctaattc attttgaatt gtttgacgca attcattcac tttttgacca
    18181 aacgcaggtt tatcttcatt cggcaaatcc ttcatcaatt tcattagtcc gctaactgac
    18241 ccttttttac ctaagtattt cactttaact tcttgcagtg cacgttcatc atttgcttca
    18301 ttaatatcta caagcgcttg ttgttttaac tctgacattg tttgttgttc agacatatgt
    18361 cactgacctc cctattcgaa ctattcattt tctatttatc atatttgttg tttaactaaa
    18421 aaaagacttt catcctctta aaaaataagg gacgaaagtc atttccgtgg taccacccaa
    18481 atttatgcct aagcatacac ttgtttaata ttgataacgg attgtctttc cgacttaaat
    18541 ctctttaagt aaccaaaaag gtgaaaagac tttaaataat tggaataact tgcagtcacg
    18601 attattctcc ctaaaacaat tatttatata aatgtccatg cctttttatt ggtttatatt
    18661 caattgtgta tgcattcacg tagaaatgtt ctgttaaaca attaattata attatatcgg
    18721 aaaactttca acacggtcaa cctttcaaat gataaagtaa aatactacct gcaatcgcta
    18781 catttaaact ttcagcttta ccataaatag gtatgattaa attttgtgta gtatgtgcta
    18841 ataattcagg attaacacct tctccctcat tacctaatag taatgcaaaa gaatcacttg
    18901 aagtaacttc tttgtatgcc actgcgtttt caagtgctgt accataaaca ggaccattaa
    18961 attgagtaat aaacgtatcg agatcttgtg tcataactgg caaatggaaa acactacctt
    19021 gactcgctcg caacacttta tcttgataag gatctgtcgt acccttctcc attattacag
    19081 catccattcc agcagcatcc gcagttctaa ttaatgtgcc taaatttcca ggatcttgaa
    19141 cacgatcgat gagcaatact tgttgtgctt tagaaatatc ataatgcggc ttctcaatga
    19201 ttgcgaaaaa cccttgtggt gtcactgtac ctgataaaga ttcagcaact ttcatgttta
    19261 tttcaaaaac ttcttgcgcg tatgcgataa tttgctgatc taatcttgcc ggttcaattg
    19321 caaataattg tgtaattaca attccacttt gataagcttc ttcaattaaa tgcacacctt
    19381 caattaaagc taatccagtt ttatccctct cacgtttctt ttttagcttg ttcgcttgtt
    19441 taattctatt attttgtgca gaagtaattt gttccattga tagctcctcg ctttattttt
    19501 aaaaataaaa atattaatca ttaataagat gaaaacattt gattgtatag ttaatattaa
    19561 ttaatcgctt ttatcactca taatatttca aattgtataa atttctttta tcgatactac
    19621 tactataaat catacgcccc aaaatatcat tattaattct tttcttcttc aaaataaatc
    19681 aaaatgatat aattgatgat tattttcaaa gcacattcaa atcaaactat gttttagcaa
    19741 tttgttgtta gcatgtttgt gttcattaaa aaaacgacca tcatcggtat catgtatggt
    19801 cgttacaaaa gctaacaata ccaattgtca taacaagtac tgcaacctct ttaaattcaa
    19861 ttatttcatg taactatagc ctatatcata tgtaattact ttgttattta taatcgggct
    19921 actttcatct tcatttttac ttctaacatg tttatgcgct gctttaaaga catcagattt
    19981 taaccaatcc gtaaaagctt gctttgattt ccaaactgtt aaaattttca cttcatcaaa
    20041 atcttcttgt tctaaagttt gtgtaacaaa catgccatca aagccttcta atgtttcaat
    20101 cccatgtctc gtgtaaaatc gttctataat atcttttgct gttccttttg ttaacgtcag
    20161 cctattttct gccataaatt tcataattat cctcttttct gtttaactta ccttaattat
    20221 ttttgcgaca acaacaattc ttttcgtcgt ttcactatat gcatcttcgc acgttgataa
    20281 agtcattatt ctatctttta ccgttacatt aacatctgaa ttaattacag atttacgttt
    20341 tgtctcatct aaaaattgtt gataatcttg atcattttca aaatctgtac gtatgtaatt
    20401 atctttagta gtagttttat atgcactaaa tacttgcaat tgatatttac catatttatt
    20461 gtcaaattca attatcttgt gtttttcata aaacgattgc tttaaataat cttctaacac
    20521 atcaaacatc gtattatcac cgacatggtg cccgtataaa atagtattat gatttaaatt
    20581 cttcaattca tttctaaaat ccataaaaat actaccttta cgtcgatgtt ctcgctcaaa
    20641 atctaaattt aaataatcgt gatttgtctt accttgtagt actggataat ttaatgatgt
    20701 tcctgataat tttatccatc caacaatgtc tttatttatt ttttcaagtg attcaaattg
    20761 tggtctcaca tgttcttgat gtttgctcat cagcatttga aatttttgtt gtaatttctc
    20821 ataatttgcg cgttcttgct tgtcttcaat atatgtttga acaattttgt aaccaaaaat
    20881 gataataatt acaaccaata aaatttgtac aatagttaaa aatcgcttca ttctcataaa
    20941 aatcctcttt tattaacgac gtttcttcag tcatcactaa accagttgtt gtaccgtttt
    21001 agattcgatt tcgttgactt tgacaaatta agtaaattag cattggacca ccgacaatca
    21061 ttaaaatagc attggctgga atttctaaag gaggctgtat cactcgtcct aataaatcag
    21121 ccactaacaa tagccatgca ccaataactg tagaaaacgg aataagtact ctgtaattgc
    21181 ccccaactag ctttctaacc acatgtggca caataatacc taaaaaggct agttgtccaa
    21241 caatcgcaac agttgcactt gctaaaaata ctgctaataa acctgttaac catctgtaac
    21301 gatcaatatt aaaaccgata cttcgcgctt gtatgtcgtc taaatttagt aaattcaatt
    21361 taggggacaa tagtaatgtt aatattaatc ccaataatgc tgatactgct aatatgtata
    21421 cgtcgctcca tattttcatt gttaagcctt gaggaatttt cattaaaggg ttttgagtta
    21481 aaatttctaa aacaccattt aataatacga ataacgcaac acctactaat atcatactta
    21541 cagcattgaa tctaaattta gaatgcaaca atataattat taaaaatggt attaaacctc
    21601 caataaaact taataatggt aagtaaaagt acaattgtgg aataaacaac atacaaagtg
    21661 ctctcattat aagtgcacct gaggaaacgc caatgatatt cgcctctgcc aaaggatttt
    21721 gtagtgctgc ttgtaataat gctccagaaa ctgctaacat tgcgccaacc atcaatgcaa
    21781 ttaatatacg tggcaatcgc aaatcaatga ttgaatccac tgcttcattg ctaccagttg
    21841 taaattttgt aaataggtca ttaaatgaca atttaattgt accggttaca aacgaaatat
    21901 aagcagttgc gattaaaatg actaacaaac ataaaaatag tagtttcttt ttatttttta
    21961 tcatcatatc aactcactat tttttatcct tataaaataa atcatataat tgagtcattg
    22021 ccttatcagc atcaacattt gctgtaatac cgaatggcac ttcctctaag tcataaacat
    22081 gattattttt cacagcttta aaatgtttcc aaatatcatt ttgtttaaat tctttttgaa
    22141 acattttctt aacttcttca ggcattccgt gtggtaatcg taaaataata tctggattga
    22201 tattcaacaa attttcagta ttagacgaaa tatattgacg atctttcact ttaataacat
    22261 tttctccacc tgctatttta actaaatcac caatatatga tttatcagtt gctactaaat
    22321 agctacccgg tacacccatt aatattaata ctttgggatg tttcttttgt ttagctgctt
    22381 tattttcaat tttttgcttt acagaattta aatggtcatt taattctttt gcttgtgctt
    22441 tacgattaaa ttgatcacct aattgtgtaa tcgacttttg catccctttt aaactatcaa
    22501 aatcataaaa gtagcctttc atatttaatt gtttgtaaaa tggttgcatt tcatctttaa
    22561 tcgttgacac actcaaaaca tgtgttggtt ttaacttttt aacagcttca acattcggct
    22621 ccattggttg accaatttcc ggtacatctt tataacgatt aggcaatgtc ttatatgacg
    22681 tgggtttgcc gacaattggt aaatccaatt tgtccaatgt cattgtcaat gcaacagttg
    22741 ttggtacgat tctgaattcg ccggatttag ttgattcttg agaactggaa gattgacagc
    22801 tggttaagat aacgacgctt atcactaaaa tggttaaata ctttatgatt ctcaaacttg
    22861 gttacctctc tttctgctgt ataaatgtgc cgaaataagt aaaatagttg ctaacacaat
    22921 gccagctgca aataacatat attgcttttt atgatttggt tcatcaagtt ctttaatatc
    22981 actagataat tgactactat tcttgctatc atcatctcta ttaaaagctg gtaacgcttt
    23041 ttctttttca actacttttt tcttttcctt ttttgatttt tcatctttat gtttcggttg
    23101 ctctttgtga ttattttcaa ccttttttat tgttggaggg ctattttcaa cttttaatgt
    23161 acctgatgct ttaggtcgct cattttcaat ctttttatta ctgtcttgtt gcactttatc
    23221 tttttgtaca ggaatatcat ttaatccttt ttcagtaaaa aggctagtac gtctttcctt
    23281 agtatctgat ttttgactat cttttttgtc atcaatatct tttttaagct gatcttgatt
    23341 attttcttga ttttgatgtt ttgaaggcac ttcttttgca tcatcatgtt gtactttttt
    23401 ctctttaaaa tgaatagaat acaccatttc tttgttatct agatttttct tttcagccaa
    23461 cgttttaaca tgcatattga ttttatccat atgctgttca attaaaattc ttactattgt
    23521 gtcatccttt tcatctttta taacaaaagg aaattcttca tttttgtcat tttgaaaaac
    23581 aagttgttct ttatctattt gaccttttag ctttacttgc aagtaaattt taccagtcat
    23641 atctgaaatc aattgcgcct tttcgtcaac tagttgttgt tcctcagtta catttttatt
    23701 ttcttgatta aaaattagta actctaacgc tttattctct tttatctttt catcaaaaat
    23761 tgatgtagtt aattctgttt tatcttttgc ttcaggttga ttaaatttgg ctgatggata
    23821 ttttatctca tcagccaaca cgctaccaat cattgttgta tatgtaagta tacctaagct
    23881 aataatagct ataatagatt ttgtagcaat ttgtttaaca tttctcatta ttccacattg
    23941 cctttagatt tttttctaac aaacaatgtg attgcgatta ataatgctaa ggatgcaact
    24001 ggtattgcat atatatatgc aggtgtacca gcatttgttt gtggattttc tactttgtct
    24061 ttattcgaac tattagattc actttgacca gtagtcgttc catcagatcc tttgtcacta
    24121 cctgaagcag aatttttatc atctttacct ggtgcattag cacctgctac atcagttggt
    24181 ccattaaatt tatatgtaat gttgtaatga tggtcatatt tgaatggctt tccatttact
    24241 ttttcatcga tataaacgtc aatttttcca tctattttac cgttcaactt acttacttca
    24301 aattcagaag tgcgttcatc tttggcagtg tttttactaa taatattttc tttatgtcct
    24361 tcgatactca ttccagtaat ccaatgactg tggttgacag ttatttgaac atacaattta
    24421 ccatttttct taatgtactt tgccggttta ttaaaatagt cattagcaat tgacgtgtca
    24481 ttggtattgt atttgtaaac ctcataattc aaagtaccgc tatctgcggc atttgcagaa
    24541 ttactgaatg tcgcgatgat gataattaac gctaaaatcg ttgtattaaa aacttttaaa
    24601 atatttttca aaacataatc ctccttttta tgattgcttt taagtcttta gtaaaatcat
    24661 aaataataat gattatcatt gtcaatattt attttataat caatttatta ttgttatacg
    24721 aaaatagatg tgctagtata attgataacc attatcaatt gcaatggtta atcatctcat
    24781 ataacaacac ataatttgta tccttaggag gaaaacaaca tgacaaaaca ttatttaaac
    24841 agtaagtatc aatcagaaca acgttcatca gctatgaaaa agattacaat gggtacagca
    24901 tctatcattt taggttccct tgtatacata ggcgcagaca gccaacaagt caatgcggca
    24961 acagaagcta cgaacgcaac taataatcaa agcacacaag tttctcaagc aacatcacaa
    25021 ccaattaatt tccaagtgca aaaagatggc tcttcagaga agtcacacat ggatgactat
    25081 atgcaacacc ctggtaaagt aattaaacaa aataataaat attatttcca aaccgtgtta
    25141 aacaatgcat cattctggaa agaatacaaa ttttacaatg caaacaatca agaattagca
    25201 acaactgttg ttaacgataa taaaaaagcg gatactagaa caatcaatgt tgcagttgaa
    25261 cctggatata agagcttaac tactaaagta catattgtcg tgccacaaat taattacaat
    25321 catagatata ctacgcattt ggaatttgaa aaagcaattc ctacattagc tgacgcagca
    25381 aaaccaaaca atgttaaacc ggttcaacca aaaccagctc aacctaaaac acctactgag
    25441 caaactaaac cagttcaacc taaagttgaa aaagttaaac ctactgtaac tacaacaagc
    25501 aaagttgaag acaatcactc tactaaagtt gtaagtactg acacaacaaa agatcaaact
    25561 aaaacacaaa ctgctcatac agttaaaaca gcacaaactg ctcaagaaca aaataaagtt
    25621 caaacacctg ttaaagatgt tgcaacagcg aaatctgaaa gcaacaatca agctgtaagt
    25681 gataataaat cacaacaaac taacaaagtt acaaaacata acgaaacgcc taaacaagca
    25741 tctaaagcta aagaattacc aaaaactggt ttaacttcag ttgataactt tattagcaca
    25801 gttgccttcg caacacttgc ccttttaggt tcattatctt tattactttt caaaagaaaa
    25861 gaatctaaat aaatcatcgt cacactcata acttaatata ttttttattt taaattttat
    25921 ttaacctatg tcatagatat ttcataatat ataacatagg ttattttttt ataaaataac
    25981 gttgcaatta actaacattt caatgtacaa tacaagtaat caattgataa tgattatcag
    26041 ttgataatat acaattagga gttgtttcta caacatgaac aaacagcaaa aagaatttaa
    26101 atcattttat tcaattagaa agtcatcact aggcgttgca tctgtagcaa ttagtacact
    26161 tttattatta atgtcaaatg gcgaagcaca agcagcagct gaagaaacag gtggtacaaa
    26221 tacagaagca caaccaaaaa ctgaagcagt tgcaagtcca acaacaacat ctgaaaaagc
    26281 tccagaaact aaaccagtag ctaatgctgt ctcagtatct aataaagaag ttgaggcccc
    26341 tacttctgaa acaaaagaag ctaaagaagt taaagaagtt aaagccccta aggaaacaaa
    26401 agaagttaaa ccagcagcaa aagccactaa caatacatat cctattttga atcaggaact
    26461 tagagaagcg attaaaaacc ctgcaataaa agacaaagat catagcgcac caaactctcg
    26521 tccaattgat tttgaaatga aaaagaaaga tggaactcaa cagttttatc attatgcaag
    26581 ttctgttaaa cctgctagag ttattttcac tgattcaaaa ccagaaattg aattaggatt
    26641 acaatcaggt caattttgga gaaaatttga agtttatgaa ggtgacaaaa agttgccaat
    26701 taaattagta tcatacgata ctgttaaaga ttatgcttac attcgcttct ctgtatcaaa
    26761 cggaacaaaa gctgttaaaa ttgttagttc aacacacttc aataacaaag aagaaaaata
    26821 cgattacaca ttaatggaat tcgcacaacc aatttataac agtgcagata aattcaaaac
    26881 tgaagaagat tataaagctg aaaaattatt agcgccatat aaaaaagcga aaacactaga
    26941 aagacaagtt tatgaattaa ataaaattca agataaactt cctgaaaaat taaaggctga
    27001 gtacaagaag aaattagagg atacaaagaa agctttagat gagcaagtga aatcagctat
    27061 tactgaattc caaaatgtac aaccaacaaa tgaaaaaatg actgatttac aagatacaaa
    27121 atatgttgtt tatgaaagtg ttgagaataa cgaatctatg atggatactt ttgttaaaca
    27181 ccctattaaa acaggtatgc ttaacggcaa aaaatatatg gtcatggaaa ctactaatga
    27241 cgattactgg aaagatttca tggttgaagg tcaacgtgtt agaactataa gcaaagatgc
    27301 taaaaataat actagaacaa ttattttccc atatgttgaa ggtaaaactc tatatgatgc
    27361 tatcgttaaa gttcacgtaa aaacgattga ttatgatgga caataccatg tcagaatcgt
    27421 tgataaagaa gcatttacaa aagccaatac cgataaatct aacaaaaaag aacaacaaga
    27481 taactcagct aagaaggaag ctactccagc tacgcctagc aaaccaacac catcacctgt
    27541 tgaaaaagaa tcacaaaaac aagacagcca aaaagatgac aataaacaat taccaagtgt
    27601 tgaaaaagaa aatgacgcat ctagtgagtc aggtaaagac aaaacgcctg ctacaaaacc
    27661 aactaaaggt gaagtagaat caagtagtac aactccaact aaggtagtat ctacgactca
    27721 aaatgttgca aaaccaacaa ctgcttcatc aaaaacaaca aaagatgttg ttcaaacttc
    27781 agcaggttct agcgaagcaa aagatagtgc tccattacaa aaagcaaaca ttaaaaacac
    27841 aaatgatgga cacactcaaa gccaaaacaa taaaaataca caagaaaata aagcaaaatc
    27901 attaccacaa actggtgaag aatcaaataa agatatgaca ttaccattaa tggcattatt
    27961 agctttaagt agcatcgttg cattcgtatt acctagaaaa cgtaaaaact aataaatcgt
    28021 ctttatattt aattattaaa ttaacaaatt ttaattggcg gatgaggtat ccagttacct
    28081 cgttcgccaa ttatttttcg caatataaaa agtcccactt aaaacaatca ttttaagcgg
    28141 gactttttat attgagtaac taaaattatt tagctgctac ttcttcgcca ttgtaagaac
    28201 cacagttttt acatacacgg tgtgataatt tgtattcgcc acagtttggg cattcagtca
    28261 tacctggtac tgaaattttg aaatgcgtac gacgtttgtt ttttctagtt ttagaagttc
    28321 ttctttttgg tactgccatg atatatcctc cttagattat aaacgaaaaa tactaaatgt
    28381 tagtttaatt aacaacatta tatcattaat taaactactt attgctcttt atcatataat
    28441 tgttgtaatt tttgaagcct tggatcaact tgtcgtgatt ctgaatcatc ttgttcttgc
    28501 tgtttagcaa gctcatctaa ttgatcctca tcgattactt cccaaccatt acctactgtc
    28561 aacatttggt cactttgctc tgaataagct ctcattggtt tctcaataat aactatatcc
    28621 tcgacaatat cctgaagatt aaccatacca tctttaataa tgtgatagtg ttcatctaca
    28681 tcatcttgat catcgttata ctgattgtac ccttctaaat caaatacttc tgtagtagtt
    28741 acatctagtg ggacttttac tggtacaaga gtacgtgcac aaggcattgt atacgttcca
    28801 gtaatgtgaa tatccgcaac gacttctgtt gacttaatgg ttaactgacc ttggattgta
    28861 attggagata aatcaattaa atctaatgat tcttttaaat tgtcaaaact caccgtttga
    28921 tcaaattcaa atggcttacc ttgatatttc cttaattgcg taattgacca tttcatatgg
    28981 cttcacctct aacaagcaca aaatttattt taactttcta agaacaagtt gtcaagattt
    29041 tttcttaaca tatcatgttc tgttacaata ttaataatga ataccgaaag gacgaattgc
    29101 aaatgaaaag cgttggctta atcacagaat ataatccctt tcataatggg catcaatatc
    29161 atattaatca atctaaaaaa cttacaaatg ctgacgttac tattgcaata atgagtggta
    29221 actttgtcat gcgtggcgaa ccagcaatct ataataagtt tactcgtgca aaaatggcat
    29281 tatcaacagc tgatttagtt atcgaactac cagcaactgc cagtttatca tctggcgatc
    29341 attttgccga actagcagtt aaagtcgcag attatatgag tgtcgataca attgcatttg
    29401 gtagtgaaaa taatgatatc aaaacattaa agcaattagc acacagcatt aatgaaattg
    29461 aacaatctga atccttttca caaaaagtaa aagaaggtaa aagctaccct cgtatcatta
    29521 gcgaattatt agagcatcat gaagcactag caagtcctaa caacatactt ggtattagtt
    29581 acctgaaagc aattgctaaa aatgctaaaa acatcaatgc aatttctatc aaacgagaaa
    29641 atgctcaaca tcatgattca ttaattcaac accatcagtt tgcaagtggt acatctatta
    29701 gaacatcaat cattagtcaa gatgatcatt ggcatcatgt ggtacctaaa gatattcaac
    29761 acttgtacgt aacacctcat ataacattaa atcaaatatt tccatatttg aaatatcaaa
    29821 tcatagctat gactacagat tcattaaaaa atatttatac tgttactgaa ggttttgaaa
    29881 atcgtttgaa atctaacatt tatgaagcaa ctgattttca tcattttgta aaattactaa
    29941 aaacaaagcg ttatacatat acgcatatcc aaagactgtt aatgaatgta ttattaaaca
    30001 ttaaacctac agatgttacg agtaacattc acgcagttaa agtattagcg atgaacgacc
    30061 gtggcagaca atatttaaag catttaaaaa cagcgttccc agaaagacaa tatataacca
    30121 atattaacaa aagcaatgca cattacttca caaatgaaat caaagcaaca cacatataca
    30181 atgcaataag cgggcaacaa caaacagatt tcaacacgcc tgtcatacag caatatcgtt
    30241 aacaaatgaa aacagtattt taggattgta aaccatgata aacctaaaat actgttattt
    30301 ttattactta aatttcttct tcaatgcctt ttcaacataa ggtggaacga attcagaaat
    30361 atctgctcga taagctgcaa cttctttaac aatacttgaa cttataaatg aataattagt
    30421 actagacatc atatataacg tttcaatttc attgttcaac tttttattca ttgaagttaa
    30481 gcgtaattca tattcaaaat cactgactgc tcttaaacca cgtatgattg ttttagctcc
    30541 tacttgttca caataatcga ctagtaaacc actaaattga tgaaccttga cattaggtaa
    30601 atgtttaaca gattgttcaa ttaaatccat acgctcttct aaactaaacg taccttcttt
    30661 tttactattt ttaagaacac agacatgaat ttcatcaaat ctatctgtac ttctctcaat
    30721 aatgtctaaa tgaccataag taatggggtc aaaactaccc ggaatgaccg ctattgtatg
    30781 ttccatgcta ttctcccttt tctaataaca atgtgtctgt caacccataa tggtaacgtt
    30841 taatcatatt aaacggttga taatctattt cttcatgatt gctaaattca caaacgatga
    30901 taccattttc tttcaataaa ttaaactctg aaattagttt taaagcttta tcaatgagac
    30961 ctttattata aggtggatct aagaaaatga catcaaattg aatatcacgt tttgacaatg
    31021 cttttaaagc tctatctgca ttatttttat aaacttcaga ttgtgcctct aaatccaaat
    31081 tcgcaagatt tgatttaata acttttacag ctttaaaatt ttgatcaaca aagattacct
    31141 tatccatacc tcgagagagt gcttctattc caagcgcccc gcttcctgca aataaatcta
    31201 aacctatacc tgacacatca tataaactat taaagatacc ttctttaact ttatccatag
    31261 ttggtctcgt attacggcct tccatacttt ctaaagcttt acttttatgt ttacctgcaa
    31321 tgacgcgcat gttgttcaca cttccaattc atttagttat ttaatataat ttattgagaa
    31381 aaaggagaat gataaaccaa tgaaacaaac atttattaca cttggtgaag gtctaacaga
    31441 tttgttcgaa ttcatgacga tgattgaata taaccatcaa cgtattgata aaattatcta
    31501 ttttcattca ccacaagctg aaaataaaaa gtcatctgta gcaatcatta tgaaccctac
    31561 aactggcaat catttccaag cattttatat catgataaac gctattaaat atccatatcc
    31621 agattcaaat aaaaagtttc aaatgataaa tgattgtgct gaaaaattcg acataccaat
    31681 tttaggtatc gatgtacagc cccctcaagc atttcatgat ttatcgttat attataatta
    31741 tttaattagt gtgttaaggc tccaaaaatg gataccagaa cttcaataat attaattata
    31801 tatttcgtgt ttctcttttt cgtaagtttt ctttaagtat ttatatggtg agccatcaat
    31861 gtgtctaacg tatttaagtt gcattagttt atgtacaatt tgatcaacat cttgctcatt
    31921 cacatacata attacaaatt tacgatctct atttgaatga acgatatgtc catattttcg
    31981 gatttgtcgt tcatgtttca tatgttttaa ataaactaca atactagttc ttgggattaa
    32041 attcatttaa acctcactcc atttattttc agcattaatc atatcatgtc tttattaatt
    32101 ttaaaatatt aagctacctt aattatgtct tatgtaattg ctcgataaat gtatttagat
    32161 tatattaaac atcattatat taattcaatt ctcaagagca tcactatatg attaaccaca
    32221 catttcgctg tagtcagaac tatgaatttg ttaagctata aaagtaaacg aggaggaatt
    32281 ttttatgaaa aaagtaaaca gaaacatttt aaatgcggta ctagtcggtg ctggattttt
    32341 aagtagtcta ttaatgatca ataaaaataa agtgattact aaaaagcaaa ccatccctgc
    32401 ttttttcaaa ggcaatgcac catatatatt tgcgcatcgc ggtggtatgg ctttaagacc
    32461 agagcaaacg caacttgcat ttgactatgc taaacaatta ggcgttgatg gctttgaaac
    32521 agatgtgaga ctcacaaaag atcaacagct tatcgttttt catgacgcaa ctgttgatag
    32581 aacgacgaac ggctctggaa aagttagtgc ccatacatta gcagaattaa agaaattaga
    32641 cgcagcttat cacttcaaag atatcaatgg actaacacca tatcgtggtc atgcacacac
    32701 agctatacta accttcgatg agttgctgaa gcaatatcct gatatgtata taaacgtaga
    32761 cttaaaagat gctcctgaat cctatgaagg ctcaatcgca ccacaaatta tgtttgatac
    32821 aattgcagaa aatcaagcat ttgaccgcgt tcttgtgaca agtttttata aagaacaaat
    32881 cgttcgtttt aataaaattg cacaaggatc cgtcgcaatt ggtgctagcc aacaggaagt
    32941 tacagaagca tttttaaaat atcatttatt aggtggtagg tactaccaac cattagcaca
    33001 aacgttccaa atgcctactc attttaaagg cattgatttg acttcatctc gttttatcaa
    33061 atggttaaat gatatgaata tcattccagg ctattatggc gtaaatagta taaatctgat
    33121 gaatgattta taccaaaaag gtgcacacac gattgtgaca gatcgaccag atttagcaca
    33181 acaattcaaa caaacaatcc cgaataaata aaaacatcga gtcagatgct agttagtggt
    33241 ttcaataacc acctttttct gactcgatgt tttttataat gaacaattac aggatccgcc
    33301 cgtcgcacaa ccactatgtg atgtttgaaa gaatggatta cctgtttcta ttttgacatg
    33361 ttctgaaaca gcgtacgcaa ttttcgtaac aacttcatct attaaatgtt gaagtgccat
    33421 ttccctagct ttgtaatgca tcacaacgtc aagcatctca tatgctcttt tgcgttgtct
    33481 tgtctctaac ataactttct tataatcagg atgatatttc ccgaatcgca ttacttcatc
    33541 atatttctct tttgatttta aaaatgattg ataaagtaaa tgtgcttcgt cattgttttc
    33601 gagcgcctgt ttagcttgct caaatgatgc ataaatatct gatgcaacta tcatatcagc
    33661 tagttcatca atatcatcta atatcgctag tgaagcttca ttaatcataa atacccctca
    33721 ttgttatttt tctaagaaaa ttagtgaata ataatctctc attacatctc cacccatctc
    33781 atcgtatttt gaatttaata gacgagatcg atgtatatct gaattcatcc aactatggat
    33841 taatgttggt acatcattaa acgcataacc aacattttga gcagttgttt tataagtaac
    33901 gtgattttta tctaattgcc ctcttaatgc gtcctctgta aattcaacac tatcagaacc
    33961 attagaggtc gcttcatata agttattaga tgcaatatgt gctaaatcgc tattgatttt
    34021 caatggtttt aatcctttta attttctcat ttcattcgtt acttcataaa gagaaattaa
    34081 ttgatttgga ttttgctcaa ctggacgctt attatgctct tctgacgtag aattagaatt
    34141 taattgataa ggttcaatat ctgctaacat ttcttttgtt aaaaatcgta cacttagcac
    34201 ctttttcgat tgttgatcag aatacacttg tgcatatatg tcgccatatt taatcagtgt
    34261 ttgtgttttt aaatcttcat ctgaaagttc aaattcatat tttttaccat caactttaaa
    34321 ggacggttct ggattaatac ttgtatgatt aaaaatttct gcagaatgtt gtcctatttt
    34381 taacggacta acattgactt tctcacctgt agcatacact gaaacgattt cttcacgttt
    34441 agttgaaaca atgtaataac tgtttttgtc tttaaacaca taatttttgt aaccatctct
    34501 aaaagggtag acacgatctg cttgtccaaa tttagaagta agcactttaa tatctttacc
    34561 tacccatgta ccaacacctt ctttaggctt tggattttca gcatggttat ttgatttatt
    34621 cacctgttca gatctattta ctttattacc tttattcggg ttctctaata catcaaattt
    34681 taatctcggt gaataaaaaa gatatattaa aaatccaact aaaaataata caccgacgac
    34741 tcttataatt aactttttca tcaatcaacc acctaaaaag tattaatact attgtaaaaa
    34801 acaacacatt aattagcaaa ttttcaacac tgacataact gtgtcgtttc gataaaacaa
    34861 aatcatcttc aggcataatt ttaacgtcat aaagattttc cctacactct atatcataac
    34921 ctatctttat gttttcaggt tgaatttcat tattaagatt aaaatatgta taaaaaaatg
    34981 gacagttaag gtatcaaatg aataccatca actgtccgac tattcttcat caaaaaacct
    35041 gataaaacaa attgccttat cagattagta tcatttgtat aagcatatta atgaccaaat
    35101 gttgctttaa tcagtgatgt cgtttctcca cctggatata atacatataa taataagtaa
    35161 actgctacac ctgtaattgc agtacagaac catataactg aagcgaatgg cccgaatttg
    35221 cggtgtacat tatatttatc tttaaatgca gtaataattt gaactaggcc tagaatacca
    35281 ccaattgttg ctaaattaat gtggaaaaat aagaaaatcg tataatattt cttaattgat
    35341 gctggtccgc caaatgctgt attaccgata aaaatcgttc tacttgcata gattaagaaa
    35401 aaagttaaag caaaaacagc tgcagctaac ataacatttt tgtgtttatt tatttccctt
    35461 ttccaaataa gtctccaacc aatggcaatt aaaattgcac taatgacaat acatgtcgta
    35521 ctaatcgttg gtaaaattgg aacgcccata tttttcatcc taacttaatt aatctagatc
    35581 aaagtaagta atgaaacaat cacagctaac acgaaaaaga tcactaaata atttagtgaa
    35641 tatataaaca tttgtgttgc ccattttgtt tgatctgaat ttttcttaaa tgttgttaaa
    35701 cctaatgcaa tccatcctaa atttaataat gtagctaaca ctacgaatac gacacctaaa
    35761 tttattagta ataaaggtac tggcaataaa ataatcaacc agataaacat actgacacgt
    35821 gtacgtttaa agcccttaac tgatggtaac attggaatat ttgcaagtgc atattcatct
    35881 ttacgtttaa tagctaaggc ataaaaatga attggttgcc aacaaaatac aactaaaaac
    35941 agcgcaatcg ctgttaaact aatttgtcct tcaattgcaa cccatccaat tagtggtggt
    36001 actgctccag gaaaactccc aatcactgtg ttccatgttg tatgtctttt agaccatatt
    36061 gagtaataag acacataacc tacaatcccc ataagaccaa gtacgcctga tggtatattc
    36121 aataaaaaca aacaaatttc tccaactaac atcataccaa aacttaatag taataaattt
    36181 tgatctgtaa ttctattatt tacagttggt ctattttgtt tactaggcat aatacgatca
    36241 atatcttggt cgtaataatt atttaacgca catgcgccac ccataattaa agtagatcca
    36301 aatagcatta ataaaatttg aggtattgat gataagaagg aatgatttgt cattacaact
    36361 gctagccatg cgcccgcaaa agccggaatt aagttacctt gaacaagtcc cattttaatt
    36421 atctgttgca attctttgaa gttaactctg ctaatatttt gtgacaaagt atgctctttg
    36481 ctcataatcc ccctccttaa atttgttcat ataagattat gatatcttag attgcataaa
    36541 aagactaggt ttaataaaat taaattgtga caaattaacg acaagagaaa atgtcaattt
    36601 tgtgacacaa ataacattta atttattgct ataatgtata tgttagaaaa ttttaataag
    36661 tagaatcatg catctaaaag agattaatat ttaagcttca aatttgagta aacgtggatt
    36721 acataattat cccaataaaa aaatcattac gattaagttc tttttatgtc gtccacatac
    36781 aatacttgta aaattaaatc atatttcctg cgttggatcc catcttttca tatcctacaa
    36841 tcaggtctat ttatagtatc atctcaaatc cggctattaa ttctaattct cagtgatgcg
    36901 tttttttatg atggggtgta taaattgttt ggcaaaaaga atttaaaatg gttaggtgtc
    36961 gtagcaacgt taatgatgac atttgtacaa cttggtggag ccttagttac caaaaccgga
    37021 tcagctgatg gttgtggttc ttcttggcca ctatgtcatg gtgcgttgat tccagaattc
    37081 tttcctattg atacgattat tgagttaagt catagagccg tttcagcttt gtctttatta
    37141 atggtcttat ggttagttat cactgcatgg aaacatatag gctatattaa agaaattaaa
    37201 cctttatcaa tcattagtgt tggattctta ttattgcaag cattaatcgg agctgctgct
    37261 gttatttggc aacaaaacga ttacgtttta gcattgcact ttggtatatc attaatcagt
    37321 ttctcatctg tatttttaat aacattgatt attttctcta tagatcaaaa atatgaagct
    37381 gacgaattat atatcaaaaa gccattaaga cgtttaacat ggttaatggc aatcatcatt
    37441 tattgtggtg tttatactgg tgcactagtg agacatgcgg atgcaagttt agcatatggt
    37501 ggttggccat tgccattcca cgatcttgta ccacattcag aacaagattg ggttcaactc
    37561 acgcatcgta tcatggcttt tatagtgttt acgattatta tgattactta tattcacgct
    37621 gttaaaaatt atccaaataa ccgtactgtt cattatggtt atacagctgc atttatactt
    37681 gttatattac aagttatcac aggtgcatta tctattatga caaatgttaa cctgataatc
    37741 gcacttttcc atgcattatt tatcacttat ttatttggta tgacaacata ctttatcatg
    37801 ctaatgttac gatcagtaag aagtgacaag caataacaaa aaagcaaacc gtaattttaa
    37861 tggcacgccc attaaaatta cggtttttta tatcaatatt taaaattaaa cctaagccat
    37921 gtaaaaacga gattacacgt caattgttgt gtaatctcgt tttattttaa tcattttagt
    37981 cagttgcttt ttcaatttcg attaataaat cgcctgtcgc tattgtgtca ccattattta
    38041 cagttacttg tttaatcaca ccgtcaaatg gtgcttgaat tgttgtttcc attttcatag
    38101 cttcagtaat tagcaacggc tgattagctt tcacagtttc acctacacta accttgactt
    38161 cagttactga acctggcatt tgagcaccga tatgacttgg attactctta tctgcttttg
    38221 gcttaacgtt cgcatttgta tgcacatttt catctttaat gtaaatacgt ctcgcttgac
    38281 cattcatcgc atagtaaatc gttctattac cattttcatc tggttcacta atcgtttcta
    38341 gtttaataat taatcgttta cctttatcga tttcgatttc tactgtttca ccattacgca
    38401 ttccaaagaa gaatgtaggc gtatcaagta acgataagtt tccgtattga tttctagttt
    38461 gaatatattg ttcatatact tttggatata gtacataact aataatatct tgctccgtaa
    38521 caggaccttg ttgctcttct tcaagcaact cacggacttt ttcaaaatca actggctcta
    38581 gatattcacc tggacgagct gttagtgctt cttggccttt taaaataacc gcttgtaaat
    38641 ctttattaaa accatttaca ggttgtccta tttcaccttt gaagaacgac actactgatt
    38701 ctgggaaatc taatttatag ccatctgtaa tcacggattg ttcatcaaga tcattttgta
    38761 ccatataaag tgccatatca ccaactactt tagacgatgg tgttactttt acgatatcac
    38821 caaataagaa attcactctg cgatacatat ctttgacttc atcaaatctt tcgcctaaac
    38881 ctaaactttt agcttgttga cttaaattcg aatactgtcc accaggcatt tcatgttgat
    38941 aaatttcagt attcggtgat ttgatatcac tttcaaagtc tgaataataa gtacgtacag
    39001 ttgaccaata atgactaagt gactccatac cttcaatatc agttctaagg tggcgtggga
    39061 agccatttaa tgcataatat aacgaattgg cgcttggctg acttgttaaa ccactcattg
    39121 aagcaacagc agtatcaatg atatcgacac cagcatctat tgcttgtttg tatgttaata
    39181 aaccattacc acttgtatca tgagtgtgaa gatgaattgg taaatctaca gctgatttta
    39241 actcaccaat caattcgtaa gcggctttag gttttaataa gcctgccata tctttaatcg
    39301 ctaaaatatg gaaaccttca cgttctaact ctttagctag tttgacataa tactctaaag
    39361 tataaatgtt tgatcgctca ggatttaaaa tgtcacctgt ataacaaata gtaccttctg
    39421 agattttgcc cgcttcttgt actgcttcat tggcaacttt catttgatct acccagttta
    39481 atgaatcgaa aattctaaag acatctatgc ctgctttagc actttcttgt acgaatttat
    39541 gaataacatt atcaggatag tttttataac caactgcgtt tgaagcacgt aacaacattt
    39601 ggaataatac atttggaata gctttacgta gacgttcaag tcgttcccat gggttttcct
    39661 tcaagaaatt atatgccaca tcaaatgtag caccgcccca catttctagt gagaaaccat
    39721 ctttaaatac gtccgctgtt ttggatgcga tattaatcat atccttagtt ctaactcgtg
    39781 tagctaataa tgattggtgt gcatctctaa aggttgtatc tgttagtaag acatcatcct
    39841 gctttttaac ccattcagct acaccttttg gacctacttc atcaagcaat tgtttcgtac
    39901 cactaaatga agcgatttta cttgaagata cagttggaat tgatgctaat tcatagtctg
    39961 gtttcggacg tttctcaaca tttgggaaac cattaattgt tacattacct atatattcta
    40021 atgttttagt acctctatct agagacggct gaatgttgaa aagttctggt gtttcttcaa
    40081 taaatttagt tgtgtaatca ccacttgtga actttttatt cttcattaca ttaattaaga
    40141 atggaatatt agttttaaca ccacgaatac gcatttctcg taatgagcgt accatttttt
    40201 cttctgcttg tttaaatgat atcgcgtgtg tagataattt tacgagtaat gaatcataat
    40261 aaggtgatat ctcagcacct tggaaaccat ctccagcatc aagacgtaca ccaaagcccc
    40321 cgcttgaacg ataagcaatg attgttccag tatccggcat gaaatcattt aacggatctt
    40381 ctgttgtaat acgacattgg atggcatagc ctaatgttgt aatatctttt tgttgcggca
    40441 tattaatctc ttcaccaaat aaatcggcac ctgctgcaac taaaatttgt gtcttaacaa
    40501 tatcaattcc tgttaccatc tctgtaattg tatgctctac ttgtacacga gggttaactt
    40561 ctataaagaa gaattcgtca ccagatacta gaaattcaac agtacctgca ttgacatatt
    40621 taatattttc catcaattga attgcagcat cacaaatacg ttgacgtaat gttgatgata
    40681 atccaactga tggtgcaact tctacaactt tttgatgacg acgttgtact gaacaatcac
    40741 gttcaaataa gtgtacgata tttccatgtt cgtcacctat gacttgtact tcaatatgct
    40801 ttggattatc aatgtatctt tctatgtaaa cttcactatt accaaatgat ttttcagctt
    40861 ctgattttgc tctatggaaa gcatcttcta attcactttc ttcacgaacg attctcatac
    40921 ctttaccgcc gccaccactt gtggctttaa tcattagcgg gaaaccagct tcttctgcaa
    40981 attcttttgc taattcatat gatttaattg gaccgtctgt accaggaata actggtaaat
    41041 ctgccttgat agccgttgta cgagctttaa ctttatctcc aaacatatct aaatgttcta
    41101 aatgaggacc aataaattta attccttctt ctgcacaacg acgcgcaaat tgttcatttt
    41161 cacttaaaaa tccatagcca ggatgaatcg catccacatt cgcttgtttt gctacatcaa
    41221 tgatacgctc aatatttaaa taactttcag caggacctaa atcacttcca actaaatagg
    41281 attcatctgc tttatatcta tgtaatgaac ttttgtcttc attcgaataa attgcaactg
    41341 tgctgatgtc taattctgcc gccgctctga atatacgaat tgcaatttct ccacggttag
    41401 caacaagtaa cttttttatt tgtttcaata gcgatacact cctcaaacta ttagaatttt
    41461 ctaactaatt agataataaa attttatctt aaagcgctct gttttgctat agttatgttt
    41521 caaattttca aaatttaaca ttcttgagac aattaaaacc tccgcttcag aaataataat
    41581 ttcaaaaatg actatgcaac aacaggtagt tcacgttttt gttgtgaaac attttcgatt
    41641 tctacaactc taaaaaatta aaaataaaat tgcaaaacat caacatttat tatcaatagc
    41701 gataacttta tcttatcatc atgattctaa tttcgccacc acatttagta atttttagtc
    41761 ataaaattta gttataatta tacgttgttt tgtttataaa atttgataat catgagtaat
    41821 ctcgtaatat caaaacaaaa aggaagttaa gcgttgtttg gttgcctaac ttccgttatt
    41881 gaactcatca gtccaatttg acaaagagta tataaatagc caatgtttag cattgcttaa
    41941 tgacagaagt catctcgtac taaatattgg ctagtatttt tttaattaaa ttgtcttctt
    42001 atatcaactt tttgttgttg tttctttcgt tgctggtcta ctttgatttg tttacctaca
    42061 atcagaagta aacccatagc aatacttaaa ctaatcattg atgatccacc aaagctgata
    42121 aatggcaatg gcacaccagt taatggaatt gttgccgaaa taccgccaat gtttacaaac
    42181 gtttgacttc caaagtatgt ggcaatccca acacacacaa gtttataaaa atatgatgat
    42241 gttttatttg caaactggaa ggcacgatat acaataaaga actctaaagt aataactagc
    42301 aatcctccga ttaaacctaa ttcttcgcaa ataattgcaa aaataaaatc tgtatgtggt
    42361 tctggtaaat agcccaattt cattgcacta tttcctaatc cttttccaaa tacgccaccg
    42421 ttacctatcg caagcaatga attggaaata tggtatccag ttcctgattc gaattggaat
    42481 ggatctgtta gcgtactaaa tctggcagtt aaataacttg gtaaccaacc agccattaat
    42541 gcaatgacaa atactactaa gaatcctagc actgctggta taccaaatct taggactttg
    42601 tttaccccaa ttcctgaata aaaaatgatc gcaactaaaa taattaatat tagtaatgtt
    42661 tgccctacgt ctttttgtag gaaaactaaa aacgtacaac ctaatgctaa tacaatagga
    42721 cttaaaatta attttggttt acttaatact ctaggcattt ttttactgat cataaatggg
    42781 atatataata tgattgcaat ttttaataac tcagatgcct gtaagttcat aaatcctaag
    42841 tttatccaac ttttagaacc attaatatct ttacctatta ctaacgtcag taataatagt
    42901 gagacgatag ttataatcat acctttttgc actttaatat tactcagtaa tttaacattc
    42961 attaaaaatg caataaaaaa tacaattata aaactcatta tgacatatgc taattgtcgg
    43021 ttgtaaaaat acgttcctgg aacatcgata ccaccagtca atgtgccttt agttgctgga
    43081 accatacttg cactatatac catcactaat ccaattaaac tcaatacaat atatgtaact
    43141 aataacggat aatcaataaa ctttgaggtt ttaccaatat accgtaaaat acttctaaaa
    43201 ttcttcatat attcagtcat ccaattctac aatttatact tcaaatgatt tcgttttata
    43261 ctttaatttt aaaaatccat gttacttatt atatacatga cacagtcata atcacaaata
    43321 ttaacaggtc tcttgaggta atttatagaa tatttgttta cttaatatcg tgaattgcta
    43381 aaacccaata aaagtgactg acatgtcatc aagaagacaa cataacagcc actttaatca
    43441 gtaattaaac tataaatata aaatcggcgc agctcaattg tcgatatctc acgccatttt
    43501 aatatgctta tatattaaac cttaaacaag tgtaaaagct tcatgtaatt ttgaaagttc
    43561 tttctcaaga cgtaacataa tttgtttgcc atcttcgcgg tcaactaaac ctaatttaac
    43621 agcaaaatca acttcttttt gaagtccaaa catttgtgta tctagtactt cttcatataa
    43681 tgggcatgaa ggtaatgtta aattatctag ttgaacttta atcagatgca agatttcatc
    43741 agcatcttta gtcaattgtt tcaaagctgc atttttcatt gttgcttgtt tcgccatgac
    43801 acgctccccc tctatataaa ttctcaagtc ttactaaaag tttatcttac taaatattaa
    43861 aaagcaagta gatttaaaat gtgtcgttag caatttgtgt taaaataatg ctatatacat
    43921 tgatagggag tgggaaaatg atacaaatta aaggagccgt aaagtttcct ataacattag
    43981 atagtacgac ttggattttc gatgatagaa aagttagcat cgaagattta gaaaaaggtg
    44041 tatttgatgg ttcaaaacca atcgactttg aagataatca tgaatggaat cgtgctatcc
    44101 tagaaggaca aactaatccg cctactttaa attcagaaat aaaatataaa aaacgtgcca
    44161 tgttagaagg ttcatttgtc attaatatga caccattttt caaacattcc gaaccacatg
    44221 actctgcaac tactattcga ttatcaaatg agcatgatca tttagatata ccgatagatc
    44281 tattacccta tttatttttc caatttgcaa aagacggtaa acgactttac agcgataatg
    44341 gtgtagatag ttttatttac aatcaagaag atggttattc atatcgaatc aaccatgtaa
    44401 ctcatattga ggtgatttaa tttgagacaa gtgcaatgca ttatctgtga tgcaaaagta
    44461 tttatagatg agcgcacaac tgagtctaaa cgacttaaaa acaatcctat tcgaacattt
    44521 atgtgtgatg attgtaaaag tcgattagac acacctaaac aacgtgcgca acactatccg
    44581 ctcgattaat atctgaataa acaagttaga aatttgaaaa tgtgaaagta acaggaaata
    44641 atagcattta aacacttttt caaatattat atgccaaaag agaagctaag caactttcat
    44701 cgctcagctt ctcttttatt atgttaaatt attcgttttc ttgcatcatt tgtttgatac
    44761 gctttgcttc tttttcacga acatttttgt ttaaaatttt ctttcttaaa cgtatacttt
    44821 ctggtgtaac ctcaacaagt tcatcatcat taatgaattg taacgcttct tccaatgtta
    44881 gaatacgcgg tctattcatt gtttgtgttt ggtctttcgt tgcagaacgt acgttagttt
    44941 gatgttttgt tttagtgatg ttaacagtta aatcattttc acgattatgt tcaccaacaa
    45001 tcataccttc ataaacttca gtaccaggtt ccatgaagtt tacacctcta tcttcaagtc
    45061 ccaaaatggc ataagtactt gcagaacctt gatccattga aattaatgca ccattacgac
    45121 gaccgccaat ttgtgcttta atacgtggtc taaattcttc aaatgtatgg ttaataatac
    45181 cgtaacctct tgtcattgac ataaattcag tcgtataacc aatcatacca cgagccggta
    45241 cattaaagat taaacgtgta agtccattat cagttgtagt catatcaacc atttcacctt
    45301 tacgtgcacc taatgattca ataacagcac ctgcattttc ttgtggcact tcacattgca
    45361 cacgttcaaa tggttcacac attacaccat ctatttcttt aataattact tgtggttttg
    45421 aaacttgtaa ttcataacct tcacgacgca tattttcaat aaggattgat aaatgcaatt
    45481 caccgcgacc agcaactacc catgtatctg gagaatctgt gttagaaact ttcaaagata
    45541 catctgtttc taattgttga tttaaacgtt cttgaatttg acgtgctgtt acaaagtcac
    45601 cttcacggcc agcaaatgga gaattgttaa ctttaaatgt catttcaaga gtaggctcat
    45661 caatacgtag aactggcaat gcttcttgat ggtcatgtgg tgttacagtt tcaccaacat
    45721 taatgtcttc catacctgaa acagcaatta aatctccagc ttgtgcttct tcaatttcta
    45781 aacgttttaa tccaaagtaa ccaaagattt tagttacacg gaagtttttc actgtaccgt
    45841 ctaatttaat tagtgataca ttatctccga cacgcatttt acctctgaat acacgaccaa
    45901 taccaatacg tccaacataa tcattgtagt ccaacaatgc tacttggaat tgtaatggct
    45961 catcactgtt atcaattgga gctggtacat aatcaataat tgtttcatat aatgattgta
    46021 aattatcatc ttgcttttca ggatctaagc tagctgtacc atttactgct gaagcataaa
    46081 caacagggaa ttctaattgt tcatcgtttg cttctaattc aataaataaa tctaaaactt
    46141 catctacaac accctctgga cgtgctgatg gtttatcaat tttattaaca acaacaacag
    46201 gtttcaggtt ttgttctagc gcttttttaa gtacaaaacg tgtttgaggc attgtacctt
    46261 catacgcatc tactactaag acaaccccat caaccatttt cataatacgt tctacttctc
    46321 caccaaagtc tgcatgtcct ggtgtatcca aaatattaat acgtgtacct ttataatcaa
    46381 cagccgtatt tttggctaga atcgtaattc cacgctctct ttcgatatcg ttagagtcca
    46441 ttgcacgttc atcgacatgt tcattttctc tgaatatacc agattgtttt aacaactcat
    46501 ctactaaagt tgttttacca tggtcaacgt gagcaataat tgctatattg cggacatctt
    46561 ctcttttatt agtcatataa atagtttcct tctttcttaa gtaaaatccc acttttaatt
    46621 acaacttttt tattatatca tataattgag aaaagataag caaaaggtgg ggataggatg
    46681 aaacaaaaaa aatctaaaaa tatcttttgg gtattttcta tattagctgt tgttttctta
    46741 gtattattta gttttgctgt tggtgcatca aatgtaccaa tgatgatttt aacatttata
    46801 ttactcgttg caacctttgg aattggattt actacaaaga aaaaatatcg agaaaacgat
    46861 tggctataag ttatatcgac aataacacgc gttgatttaa gaccacacat gttcatgatg
    46921 aacatttaac tggttttata ttggcgcgtg tttttattat ttaaggtata aaagcgtaat
    46981 atgttaccta attgttagga ctacatatta cgcttttggt aattattttg attttctttt
    47041 aaatcgttgt tcatgtaatt gtattaacgc atcatggtgg ggctctaaat aatcattgct
    47101 aatttcttga tggagaccac gatttccaac taagattgaa tttggaccac taattgttaa
    47161 tggttctcct agtaaattgg aagcttgtcc atttacttca tataaaataa ccaatccgcc
    47221 agcaaaatcc catggttgaa gtcttggcgt catatatgct tctaaattac ctgtagcaac
    47281 tgaaacgatt tcaagcgctg cactaccata tgcccttgca cttctagaat cattaacaat
    47341 ttctttaaag atttctccta aaattggttt agttaaccag ttcggattga tcccaataat
    47401 gctttgtctt agattagaat cattcaatgg tttcaagggt tggctaccac gatatgcacc
    47461 ttcccctact ttagcatgat ataagacatc agccataaca tcatatacaa aacctgcata
    47521 aggtttacca tcgatataaa taccaattga aattgcgaaa ttttcttgtt gatgaacaaa
    47581 attcaatgta ccgtctattg ggtcaacaat ccataccgta cctttggaag tatcgatgtc
    47641 atgaccatgc ccttcttcac ctaatacttg atgattggga tatgtttcta aaattgtatc
    47701 aaaaatgaaa tcttctgttg ctttatctac atttgtaaca aggtcattcg gatttgactt
    47761 tgtttcaatt gttaaatttt gctccatcaa ttgtttaatt ctaattcctg cttcttgaat
    47821 aagtccttgg gcaaatccat aaagtgccat aacatcatct cctaacatct ttattataca
    47881 tcaacatttt ataaaaataa cttcttatga taaaaatgaa aatactaatt ttaatgagag
    47941 cgtcttaagc cgcaattgat aaaacatatg ctacaatatt tttaaacact atacaggagg
    48001 tactctatga caaaatatac atttaaacct aaagatttca aagcgttcaa cgtagaaggc
    48061 ttagacgcac gaatggaagc tttaaacgaa tacatacgac cacaactccg tgaattagga
    48121 gaatatttta gtgacttctt tacaagtcaa actggtgaaa cattttatcc tcacgtagca
    48181 aagcatgcta gaagaagtgt gaatcctcct aaagatacat gggttgcttt tgcaacaaac
    48241 aaaagaggct ataaaatgtt acctcatttc caaattggta tgtttgaaga tcaactgttt
    48301 gttatgtttg gaatcatgca tgaagcaaaa gataaagcaa cacgtgcaaa agtttttgaa
    48361 agaaaattta aagctattca acaattacct gatgattatc gtgtttgctt agatcatatg
    48421 aaacctgata aaccatttat taaagattta acggatgatg atttaataga agcgatacaa
    48481 agagccatca atgtgaaaaa aggtgaattc tttatagcgc gtgcaatcac accacaagat
    48541 aaaagattaa aaagtgacaa agcatttatt gcatttttag aagaaacctt cgatcagttc
    48601 ttaccatttt attctgcata aataactttg tttaaataat agagcactta atcacatcca
    48661 tgatttcgtg ctcttttttc ttaatattaa atcgaacgtt caacataata attcatactt
    48721 ttaaaaaaat taaaataaat ttaggttgac ctaaacattt tattaggtta ttatattgtc
    48781 cataagaagt agaggtgagt caaaatgaat aataaacgac attcaacaaa tgaacaatta
    48841 agtttagacg aaataaacaa tacaattaaa ttcgatcatc gcagttcaaa taaacagaaa
    48901 tttttatcat ttcttggacc tgggttatta gtcgctgttg gttacatgga tcccggaaac
    48961 tggataacat caatgcaagg tggcgcccaa tatggctata ctttgctatt cgtaattctt
    49021 atttcaagtt tatcagcaat gttacttcaa agcatgacag tgagattagg aatagcaaca
    49081 ggtatggact tagctcaaat gacacgacat tatttatcaa gacctattgc tataatcttt
    49141 tggatcattg cagaactagc aattatcgct acagatattg ctgaggttat tggtagtgct
    49201 attgctctta atctcctatt taacatacct ttaatcgtcg gtgcactaat aactgtactt
    49261 gatgtatttt tactactttt tataatgaaa tatggtttta gaaaaattga agctattgtt
    49321 ggtacattaa ttttcacagt gttattcatc tttatatttg aagtctatat ttcatcacca
    49381 cagttgaatg ctgtgttaaa tggatttata ccacatagtg aaatcattac aaataacggc
    49441 attctctata ttgcattagg tattattggc gctacaatta tgcctcataa tttgtactta
    49501 cattcatcaa ttgtacaatc tagaacatac tcaagacata acaatgaaga aaaagcgcaa
    49561 gcgattaaat ttgctacgat agattcgaac attcagttat caatcgcatt tgtagtcaat
    49621 tgcttattat tagtgttagg agcatcacta tttttcaact caaatgctga cgatttaggt
    49681 ggtttctatg atttatatca cgccttaaaa actgaacctg tactaggtgc aacaatgggt
    49741 gcaatcatga gtacattatt tgcagttgca ttattagcgt caggtcaaaa ttcaacgatt
    49801 actggtactt tagcaggaca aattgtaatg gaaggatttt taagattaca cataccaaat
    49861 tggttaagac gtttaattac acgttctctt gctgtcattc ctgttatcgt atgcttaatc
    49921 atttttaaag gtaatgcagc taaaattgag caactacttg tattctctca agtattttta
    49981 agtattgctc tgccattctg tttaatacca ttacaattgg ctactagtaa taaagacttg
    50041 atgggacctt tctataataa aacatgggtt aatatcattt catggacact tattattatt
    50101 ctaagtattt taaatgtcta tttaatcgtt caaacattcc aagaactaca aagttaaaaa
    50161 taaaaccatt aaaatgttgg ttgtatctaa taattaacaa cttaacattt taatggtttt
    50221 tcttttgcta tgaacatatt aatgataaat atactcatcg tccttatgtg ttgtttcttt
    50281 tgattctttt ttagaaatca acataatcat cacgataacc cataagacaa ttgcaattaa
    50341 attaagagcc atcaaactta caattatcgc gataacaaaa agtataatcg ataattttct
    50401 acgtccttta atacaaattg tcgcaaatat accataaagt gttgaaatta ataaaataat
    50461 tgctaatgca cccattgttg tctgaagcat atctaaagaa accttgccac cgttctgaga
    50521 taattgcttc gctaattctt cacgttgtga tgcattacca ctttttaaag aaaaatagga
    50581 tagccccata actaataaat aaataatact aatactattt gcaatccaag ccatgattaa
    50641 ttcaggtttt ctattcatct taaaatcctt tctttgttta atattttaat aagtatacta
    50701 cgagcctata gatatacgca cattaaatta cttgtatatt aacatatttt tcatctaatt
    50761 tttataataa acatctgaat tcagattgat gataatttag gtgtatagta atgagaaatc
    50821 gtcatttaaa attatgacac ctaaacactt taattaaacg ttatcgataa ttaacctcat
    50881 ataaaaaccc agattaataa gcaaaagacc tctgcttact aatctgggta tcatcaatat
    50941 ttattcttgc tcttttttgt ccttaacttc acttgaattg cgttcttcag aaatatcatt
    51001 gttgtaaatt tgacgtcgct cttcaaaatt cattcgacgt tgactaactg cgctaggttg
    51061 atttttccta cgttctttca ttcgttggtt ataagcttta cgtttttctt ttttcttagc
    51121 tttaatttca gctttttctc gcttttgttg tgctttcaat tcttttggat caacttcagg
    51181 ttctgcataa taaccatcat cttcaatacc tagttcttca gtactttttt gtttgtattt
    51241 agcctgtctg gataatactg atggttcttc ctcagtgcgc tcatcatgat tatattcact
    51301 ttcaactgca cgtttaggaa attgatcata tttatctctt tcctgttctt gatcatatac
    51361 agtatttgat ttatccaatt ctttttcata tttaggcaca tcataataat catctttcgg
    51421 ttgggggcgc tcatacacag gttcacgata atcataaatc ggtccgttat actcttcata
    51481 atattgtggc gcaaccattt caactttatc ttttcttata aatagcatca tcgcaataat
    51541 aaagaataat atcggaatga ttaaagtaac gataagtaaa actaaaggaa tcgttacaat
    51601 tgctgatatt aagaataaga aacctgacaa gattcttata ttcatagata tgagagctaa
    51661 aaatgaaatc aacaaacaaa caataaagta aacgataata gcccatatac cattttgtaa
    51721 ccatattaca aattgtgttg tattatagcc atttccagct aataattgct ggataaatgc
    51781 attattgttt aatgtatttt caagattagc aatcgatgtg ttattactga atgaaacaag
    51841 tgctataaac atcgtaatga cagtaagcac taataacatc acccaacata accaacctag
    51901 aactttttca gttaatctac ttactggacg tttaatttga gtaaattgtt ctccagtcat
    51961 tcgttacaac tccttatagt acttatcccg ttattataac taaatataca gtaaataact
    52021 actatttatg attttatttt aatgacattt tgaaattcaa aaagttttca ttgtattcac
    52081 ttaaaacttc aggtcctaaa tctttataaa cttcaaggcg ttcttgctct ttcttagtcg
    52141 gataaaaacg atggtcgtct ttaatctctt taggcaacaa ttgtcgagca gccttgtttg
    52201 gcgttgcata gcctacgaat tctgtatttt gcttgttatt tttagcatct aataaaaaat
    52261 tcataaattt atatgcaccc tctttatttt gtgccgtttt tggaattacc atattgtcga
    52321 accataaatt cgatccttct ttaggaataa cataattata tttatcccct tcttgcacta
    52381 gaggtgctgc aacaccactc caaacaaccg ctatgttacc ttcattttgt tgaagcatca
    52441 tggtaatttc atcacctacg acacctctta cttgtggtgc tagtttggtt aaatctcgct
    52501 ctgcttcttt taaatggtgc gaattacggt cattaagatt atacccaagt ttattcaaac
    52561 tcatgcctat aatctctcta gcaccgtcaa ctagtaaaat ttggttttta aatttaggat
    52621 tatacaatga cttccaacta tcaaatgatt catttggata cttttcttta ttatataaaa
    52681 tacctacagt tccaaagaaa taaggtaaag aatatttatt gcctctatca aatgacatat
    52741 tcatataatc tgaatctaaa tttttaatat taggtacctt attatgatct attggtaaca
    52801 ataaatgatc tcttttcaat ttttgaactg tatattcact aggaaaagca acatcataat
    52861 gtgtaccgcc attgcgaatt ttggcttcca tcgcttcatt tgaatcgaaa gtttcataaa
    52921 cgacttgaat gccagtttct ttttcaaatt tcttaattaa ctctggatca atatattcgc
    52981 cccaattgta tacgtaaatt ttttgatttg tatgcacttg ttctttagat ttaaaccaat
    53041 gacttaaagt aagacaaagc atacccacaa ctaatgcacc tataatgagt tgtaaaaatc
    53101 gtttcattat tttacacctc gcttgattag ttttttctta tttatcacgt attgaatcaa
    53161 ataatatcct agtattccta atacaataac agcaaacaat aatgttgaaa tcgcattaat
    53221 ttccatacta attccttttc tcgccatagc ataaacttca actgataaca cactaaagcc
    53281 attaccagta acgaagaaac ttactgtgaa atcgtctagt gaataagtta aagccataaa
    53341 gaatcctcct ataatagaag gtaaaatatt aggaataata atgttgctta ataattgtgg
    53401 ttcagtcgct cctaaatctc ttgcagcatt taacatatta ttattcattt catacagttg
    53461 tggtaagacg ataatcacaa ctataggtat gcaaaatgca atatgagata ttagaactgt
    53521 ccaaaatcct aaaccaagac cagtaaaatg gccaatcgtt gtaaacataa ttaagaatga
    53581 tgcacctatg acaacgtcgg atgataccat caagacatta ttcaatgtta gtaaagttac
    53641 tttaaacttt ttatttctta aataataaat agcaatggca ccaaatgtac caataactgt
    53701 agaaattgag gctgctaaaa gtgctacagc tatcgtatta aaaatgaccg acattaaatg
    53761 atcattttga aataatgatt gataatgctc taatgtaaaa tgttcaaagt gaatcatatt
    53821 accagccgaa ttgaatgaat agaacattaa aaagaatatt gggatgtata aaatcgctaa
    53881 aagtatcccg atatacagct ttccatacca tttcatatga ttcaccctct cccattagat
    53941 gattttgtaa tgattaaaat gaatgccata aatacaatta agaatatagc tatagttgat
    54001 cccataccat aattttgaat tgttaaaaat tgttcctcta ttgccgtacc tatatttatg
    54061 actttattac ctgcaattaa tcttgtaatc ataaataatg aaagtgatgg aataaaggtt
    54121 acttgaatcc cagtcataac accttctttt gttaacggca tgattacttt tctaaaagta
    54181 tagaaaggac tggcacctaa atcacttgag gcctgcaata aattattagg aattgctttc
    54241 atgctattaa atataggtaa aatcataaat ggtatataaa tgtaacttgc cactactaaa
    54301 aacgcaccag ttgtaaataa caaattgaat gatggtaaat taaataagtg gaaaaattga
    54361 ttaatcacgc catcatgact taataaacct ataaaagcat atgtctttaa caataaattt
    54421 atccatgttg gaataatcat tatcattaat aagatatttt gaaatttcga acgagtaata
    54481 taataggcag ctggataact gatagtcaag gtaataattg ttattgaagc ggcatataaa
    54541 attgaatatg caaacatttt caaatatttt gtagtaaaaa tttgttgata attcgtgaaa
    54601 ctaaaatgtc cattgatatc taaaaatgaa aaataaatga gtaatacaac tggtacaata
    54661 ataaatataa ccatccatag taaatacggg attaagagaa atttattagt attacgcatt
    54721 gtctacttcc tcatagcttt caatacgttt atcaaattct tcttctgttt ctccaggaac
    54781 cataatatga atggcttcag gatcaaaata aagaccaact tcactgccta cttcagcttt
    54841 tttagtcgtt tgtattaccc attcataacc tttattgtct atacaacata tttcatagtg
    54901 gacccctcta aataacatag aatcaacagt tgctttaaat aatccttctt cagctttgat
    54961 taatgatata tcttctggtc gaataacgac ttctactttt ttattttcag gaatacccat
    55021 atcgacacat tcgaaatctt gcccataaat attcacgaca taatctctaa ccatgcgccc
    55081 ttcaacaata ttagattctc caataaaatc agctacaaat cgattcactg gttcgtcata
    55141 tatatctgtt ggtgtgccaa attgttgaat tttcccatct tttaatacaa aaagaaagtc
    55201 acttaatgct aacgcttctt cttgatcatg tgttacaaat ataaatgtaa tacctaatct
    55261 agattgcaat tctcgtaatt catattgcat ttcagtacgc aatttcaaat ctaatgcgga
    55321 taaagattca tctaacaata atatttctgg ttcatttacg atagcacgtg caattgcaac
    55381 acgttgcttt tgtccgccac tcatttcatt aatatttctt ttttcataac ctgaaagttt
    55441 tactaatttt aatgcctcag ttactttttg atcaatttcg gtttttgata atttttttaa
    55501 tttcaaacca aaagcgatat tatcatagac gtttaagtgt ggaaataatg cataatcttg
    55561 aaagactgta ttcactttac gtttatttgg tggtaaatta ccaattggtt tgttttgata
    55621 aatcacttca ccactgtcag gatattcaaa ccctgcaatt aattttaaaa ttgttgtttt
    55681 accacaacct gaaggaccta ataatgtata aaagtatcct gattcaatat caatatctat
    55741 gtcatctaag atattaagat catcatagct tttactaact gattttaatg ataataacgg
    55801 ttccaaataa gatcccccct ataaatatga agctgtcgct acaataagta ttcgtgtttc
    55861 attgtttgat tcgtttgaca aacgatgatt atctcgtgct ttaaaataca aaacgtcttc
    55921 ttcttgtgct tgatatatct ctttgcctaa attaagtgtt atctgacctg acatacaata
    55981 aataaacgta tcagactctg atggattaaa atttttatat gatgctccag gctttaaagt
    56041 taatattaat ggctccatat catattcatt tgactttgaa actaaccaat ttaatatata
    56101 accttcatca tactcatcat aaataacttg ttcttccttc ttgtataata ctttttcatt
    56161 ttcactgtct ttaaaaaatt cacttggcgt cgttcctaac acctctataa tatttaagaa
    56221 agtttccata cttggtgagg catgttcact ttctatttgt gaaatgtagc ctttcgataa
    56281 gtctgtacgt tcagcaagtt cttcttgcgt taaattttta attcttctaa gatttttaat
    56341 tttattacct atgttcattt atttttccac ctaaaaaagc tatattaagt ttacttttat
    56401 caaacatttt gtttaacact tgataaaggt atcaaaatat agctttatta gcaatagttt
    56461 taaagaaaaa ttcctattaa attgtgttaa tttcgaacga attatttttt agctgttact
    56521 ctttgttctt cagagtgagt ttttacatct tgaactgctt tgtaactatt atagccacta
    56581 cttttttcga aagtattaaa aatttgtttt tcctctgctt tagcaggcac aatttcttta
    56641 aatcttttat atgcacccat aaagtcgcct gccgtcacgc cggattcata atacttctct
    56701 acatgattaa agaaatttat cactgaaatc atctcttcat tactccagtc taaatcaatt
    56761 ggatactcat attccattgt tgaaccctcc atttcaattc ttttgttagc atcgctagta
    56821 taacatgcat gtcacatgtt aacgatatct caaatcaatt tccttacatt actttaccta
    56881 aaacaaaaaa gccgtagcat atgctacagc ttttaatgac tgaatcttta tagataatca
    56941 gttattacat tgtatggatt gggtatccga tagctttttc tgctgcttcc atagtcatct
    57001 cacctaatgt tggatgtgca tggattgtta atgcgatatc ttcagcattc ataccagctt
    57061 caattgctaa acctaattca gagataatat ctgatgcacc agtaccaact acttgagcac
    57121 cgattaaagt atcatcttct ttaagtgtaa taagtttaac aaatccgtta gtatcatcta
    57181 atgataatgc acgaccattt gctgcatatg ggaatttaga agctttaatt gctaaacctt
    57241 cttctttagc ttgcgcttct gaataaccaa ctgtagctaa ttctggttca gtaaagcata
    57301 ctgctggcat accaatgtaa tcaacttcag cagcttgacc atcaattgct tcagcagcaa
    57361 ctttagcttc atagctagct ttgtgagcaa gtggtaaacc tggaacgata tcaccaattg
    57421 catagatatt gctgatagac gtacggcttt gtttatcaac ttctaataat ccacggtcag
    57481 cgaatttaac acctaattct tctaggccta attcgtctgt gtttggacga cgacctacag
    57541 ttactaatac ataatcagct tcgattgttt tctcttcgcc tttagcttca taagtaactt
    57601 taactccgtt atctgtttct tcagctgatt tagccatagc ttcagtaacg atttcaacac
    57661 ctttttcttt catacctttt ttaacaggtt gtgtcatttg tttttcgaag ccacctaaga
    57721 tatctttagc accttcaagg atggttactt ctgaaccaaa gttagcaaat gctgtaccta
    57781 attctgatcc aatgtatcct ccaccaacta caactaattt acctggtact tcttgtaagt
    57841 ttaaagcacc tgttgagtcg ataacacgtt taccgaattt gaaattagga atttcaattg
    57901 gtcttgaacc tgttgcaatg attccatttt taaagttgta tgtttgtgcg ctcttttcgt
    57961 ccataacacg taagctattg ttatctacga aatatgcttc acctttaacg atgttaactt
    58021 tgttaccttt aagtaagcct tcaacaccac cagttaattt attaacaact gatgatttga
    58081 attcttgaac tttttggaag tttaaagaaa cactttcagc aataacacct aagttttcag
    58141 aatgttgtgc ttcaacaaaa cggtgagaag catgtagtaa tgcttttgaa ggaatacatc
    58201 ctacgtttaa gcaaacacca ccaagattac ctttctcaac gattgttact ttttgtccta
    58261 attgagctgc acgaattgct gcaacgtatc caccaggacc tgctccgatt actatagtat
    58321 ctgtttcaat tgggaaatct ccaactacca tgttttaccc ctccattaat aataattctg
    58381 gattatttaa taaacgttta atgtgattca ttgcattttg gccagttgca ccatcaattt
    58441 gtctgtggtc aaagcttaat gataatgcta atactggtgc tgcaacaatt tctccatctt
    58501 taacgatagg tttttgagca atacggccaa ttcctaagat tgctacttct gggtgattga
    58561 taactggagt gaaccattgt ccaccagctg aaccgatatt actgattgtg catgtagcac
    58621 ctttcatttc atcggctgtt aatttaccat cacgtgcttt aacagctaat tcattaattt
    58681 catctgaaat ttggaaaata gacttacgat cagcatgttt aacaacaggt actaataatc
    58741 ctctatcagt gtctgctgca ataccgatat tccagtaatg tttatgaacg atttcaccag
    58801 cttcttcatt gaatgaagtg ttaagtgctg ggtatttttt caatgcagaa acaagtgctt
    58861 taacaacata aggtaagaat gttaacttag taccttgttc agctgcgatt tctttaaatt
    58921 tcttacggtg atcccataat gcttgaacat caatttcatc cattaatgtt acatgaggtg
    58981 cagtatgctt agagttaacc attgctttcg caattgctct acgcatagca gggatttttt
    59041 cagttgtttc tgggaagtcg ccttctaatg ttactgctgc aggtgctgca ggagtttcag
    59101 caacttcttc acttgtagct gaagcagctg attcatttga agctgttggt gcaccaccat
    59161 ttaagtatgc atctacatct tcttttgtaa tacgaccatt tttaccagat ccagaaactg
    59221 ctttaatgtt aacacctttt tcacgtgcgt atttacgtac tgaaggcatt gctttaacag
    59281 ttctgttttc atctacttct tcagtttgag tagctacagg tgcttgctct gctggcgctt
    59341 cctctttcgc aggttcttct ttagatgatg aatcatcatc atgaccttta aattgcatat
    59401 cttctgcatc aggtgcatcg attttaacaa taacgtcacc aactacagct actgtacctt
    59461 cttctaccat aacttcttct acagtaccag atactggtga tgggatttct actactgatt
    59521 tatcgttttg tacctcagct aaaacatcgt cttcttcaat agtatctcca gctttaacaa
    59581 accattttac aatttcacct tcgtggatac cttccccgat atcgggtaat ctaaattcaa
    59641 atgccacgtt tttgccctcc taagatttcg taaattaaac atttcatttt aatcaatgag
    59701 actaaaatac gctaacttcg ttaactttta aaatgtatta aaattctaaa gtttcttttg
    59761 ctttttcgat gatgtcattt ttgtttggta accaaacatt ttcagcttga gtgaatggat
    59821 aaattgtatc tgctgctgca actcttccaa taggtgcttc taatgaaagg attgcacgtt
    59881 cacttaattc agctacaact gctgcaccaa caccagcttg acgttgtgct tcttgaacta
    59941 caactgcacg accagttttt tcaactgaag ctacaattgt gtcaacatcg attggttgaa
    60001 cagtacgtaa gtcaattact tcaacagaat aaccatcttt ttcaagttct tctgcagctt
    60061 tcattgattc ttgaaccatc gcaccgtatg tgatgattga aatgtcatta ccttcttttt
    60121 tcacattagc cttaccaatg tcaattgtat attcttcttc aggtacttct tcacggaatg
    60181 aacgatacaa tttcatatgc tctaagtata cgactgggtc attacttcta atagaagaaa
    60241 ttaataaacc tttcgcatcg tatgggcctg aaggaataac aacctttaga cctggagatt
    60301 gagctaaaat accttctaag ttatctgcgt gtaattctgg tgtgtgtacg ccaccaccaa
    60361 atgggctacg aattgttaca ggtgcagttt tagtaccgcc tgaacggaaa cgagtacgtg
    60421 caatttgtcc agcaatcgca tcaaatactt cgaatacgaa acctaagaat tgtacttcca
    60481 taaccggacg gaatccttca actgcaagac ccatcgctaa accaccaata cctgattcag
    60541 ctaaaggtgt atcgaatact ctatcttcac caaattcttt ttgtagtcct tcagtaacac
    60601 ggaaaacacc gccgttaaca ccaacgtctt caccaaaaat taaaacatct tggtcatttt
    60661 taagttcagt tttaagcgca tcattaatcg cttgaaccat tgtcatttgt gccatggctt
    60721 acttcgactc cttctctttg taaatttcat attgttctgc taagttttga ggcatatctt
    60781 catacataat ttccattaga gaagtaacag tttgtttttc agtgttatca gcctctttaa
    60841 ttgctgcttt aatatcagct tttgcacgtt caataacttc attttctttg tcttcattcc
    60901 ataaaccttt gttttcaagg aatttacgga aacgtactaa tgggtctttt ttctcccatt
    60961 cagcatcttc gtctgaagtt ctgtaacgag ttggatcgtc accagccatt gtatgaggac
    61021 cataacgata tgtcatagtt tcaattaatg ttggaccttc acctgcaact gcgcggtcac
    61081 gtgcttcttt agttgcttga tatacagcta acgcatccat accatcaact tggataccag
    61141 gaatacctac agcaattgct ttttgagcta atgtttcagc agcagtttgc ttgcttcttg
    61201 gtgttgaaat tgcatagtta ttgttttgaa taacgaaaat tgcaggtgct ttataagctg
    61261 ctgcaaagtt aataccttcg tagaaatcac cttgtgaaga accaccgtca ccagtgtaag
    61321 tgattgcaac tgcattttta ccacgttttt taagtgcaaa tgcaacacca gcagcttgaa
    61381 tgtattgtgc accgataata atttgtgggc ttaatgcatt aacgccttca gggaattgat
    61441 ttcctttgaa gtgacctctt gagaataaga aagcttcagt taatggtaaa ccatgccaaa
    61501 taatttgagg aacatctctg tatcccggta aaatgtaatc ttctttttct aaagcgtatt
    61561 gtgacgctaa ttgtgatgct tcttgaccag cagttggtgc atagaaacct aaacgtcctt
    61621 gtctgtttaa tgagatagaa cgttgatcaa ggatacgagt ccataccatt ctttccatta
    61681 attccactaa ttgttcatcc gtaagatcag gtactaagtc ttcatttacg acgttaccat
    61741 tctcatccaa aatttgaacc atttcaaatt tcgattgagt atcatttaaa acttttactg
    61801 catcgaattg ggcttgtaac ttaggagcca ttcaattcac catacctttc ccataattgt
    61861 atagaaattc attttatcct tactaattgt accacaaata aatgggactg ttaaaccatt
    61921 tgaaaaagtt ctgttttaat aaaaaaagat acagataata ggaactgtat tagttgtttc
    61981 ctaccatctg tattatatta attatttaaa ttgatcaacg tcttgctttt ctttaccaac
    62041 cttatttagt acttttgtat acttatctga tacttctttt aactttttat agttctgttc
    62101 tattgctttt gatttttcgt taacaccttg ttgtgtcgcg tcattttgat ttaaatattt
    62161 aaataatgtt ttctctgagt ttacagcctt tttatatgct ttcgcgtaat cactgtgtaa
    62221 cttatatttt tcttttaata catcatctaa ttgttttact tctttacgtt taacatcatt
    62281 atcaatgtta tcaacgtgac tttttgcttt cttaaagtct tgttcagact tcttaattgc
    62341 gtcttcttct ttttcaaatt cctttagacg atcatcggca tttttaatta aatcctttac
    62401 tgctttaccg cgaactgctg tatctttact attgacatct tcagttaatt ttttcttttt
    62461 ctcatctaat tcagcaattt tcttaccaac agttttaatt ggttcttcat catctttaat
    62521 tttatccact tgctttaaat atgccttaat ttctttttta tccgtagtac atcctgcaag
    62581 caagacacta gatgctaata ctactgcgat tgtttttcca aatttcatta cttgtacctc
    62641 cacttgctat ttaaattaaa tgataaataa aatctgcttg gaaatcaatt ttaaacaatt
    62701 acattacaca tcttcattac taagtatatc tgttgacagt tacgtcattt ataatataat
    62761 ttttaaagtc aagatgataa ataaatttct aaagtatcat tgcttgaaac caacacttta
    62821 atcatttttt aagcaattag catatctgaa aatttacata tactatctaa aataaacatt
    62881 ggcagtagtt ggttaacttt gtagatattt acacataaaa atgtatgagt atcttaacaa
    62941 cctttttact gtcattcaac taaaacaaat taaaataagg aaggtgcaat atatgttaac
    63001 aatgaaagac atcattagag atggtcatcc aactttgcgt caaaaagcag ctgagttaga
    63061 attaccatta actaaagaag aaaaagaaac attaatcgcc atgagagagt ttttagtaaa
    63121 tagtcaagat gaggaaatcg cgaaacgata tggtttacgt tcaggcgttg gtttggctgc
    63181 acctcaaatt aatatttcta aacgtatgat tgctgtttta ataccagatg atggcagtgg
    63241 caaatcttat gactatatgc ttgtgaaccc aaaaattgta agtcatagcg ttcaagaagc
    63301 ttatttacca actggtgaag gttgccttag tgtcgatgat aatgttgctg gtctagttca
    63361 ccgtcataat agaattacaa ttaaagccaa agacatcgaa ggtaatgata tacaattacg
    63421 actaaaagga tatccagcaa ttgttttcca acatgaaatt gaccatttaa atggtgtaat
    63481 gttctatgat cacattgaca aagatcaccc attacaacca catacagatg cagtagaagt
    63541 ttaaaacaca tttttctaat tatcaaagct taggataaat atgatgtcct aagcttttcc
    63601 ttttacaact ttttcgaata aacaacagtt aaatatattc acctttctac caaacttttt
    63661 atcccctcat ttaaatttta ccgtttcata taaaatcctt taattctttc ttaacattaa
    63721 tttataatct c
//
LOCUS       DALAAR010000006        61668 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000006 DALAAR010000000
VERSION     DALAAR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 61668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.006"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            89..436
                     /gene="sarR"
                     /locus_tag="O2458_000374"
     CDS             89..436
                     /gene="sarR"
                     /locus_tag="O2458_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCZ3886263.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(625..1461)
                     /locus_tag="O2458_000375"
     CDS             complement(625..1461)
                     /locus_tag="O2458_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCZ3886264.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1461..2075)
                     /gene="ureG"
                     /locus_tag="O2458_000376"
     CDS             complement(1461..2075)
                     /gene="ureG"
                     /locus_tag="O2458_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCZ3886265.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2088..2777)
                     /locus_tag="O2458_000377"
     CDS             complement(2088..2777)
                     /locus_tag="O2458_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCZ3886266.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2770..3222)
                     /gene="ureE"
                     /locus_tag="O2458_000378"
     CDS             complement(2770..3222)
                     /gene="ureE"
                     /locus_tag="O2458_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCZ3886267.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3235..4950)
                     /gene="ureC"
                     /locus_tag="O2458_000379"
     CDS             complement(3235..4950)
                     /gene="ureC"
                     /locus_tag="O2458_000379"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCZ3886268.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4947..5357)
                     /locus_tag="O2458_000380"
     CDS             complement(4947..5357)
                     /locus_tag="O2458_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCZ3886269.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5371..5673)
                     /locus_tag="O2458_000381"
     CDS             complement(5371..5673)
                     /locus_tag="O2458_000381"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCZ3886270.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5933..6838
                     /gene="yut"
                     /locus_tag="O2458_000382"
     CDS             5933..6838
                     /gene="yut"
                     /locus_tag="O2458_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCZ3886271.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7055..8209
                     /locus_tag="O2458_000383"
     CDS             7055..8209
                     /locus_tag="O2458_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ3886272.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8423..9331
                     /locus_tag="O2458_000384"
     CDS             8423..9331
                     /locus_tag="O2458_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3886273.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9729..10670
                     /locus_tag="O2458_000385"
     CDS             9729..10670
                     /locus_tag="O2458_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ3886274.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10791..11345
                     /locus_tag="O2458_000386"
     CDS             10791..11345
                     /locus_tag="O2458_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCZ3886275.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            11417..12187
                     /locus_tag="O2458_000387"
     CDS             11417..12187
                     /locus_tag="O2458_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3886276.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12359..13156)
                     /gene="fdhD"
                     /locus_tag="O2458_000388"
     CDS             complement(12359..13156)
                     /gene="fdhD"
                     /locus_tag="O2458_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCZ3886277.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13446..14228
                     /gene="modA"
                     /locus_tag="O2458_000389"
     CDS             13446..14228
                     /gene="modA"
                     /locus_tag="O2458_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3886278.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14242..14913
                     /gene="modB"
                     /locus_tag="O2458_000390"
     CDS             14242..14913
                     /gene="modB"
                     /locus_tag="O2458_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCZ3886279.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14914..15519
                     /locus_tag="O2458_000391"
     CDS             14914..15519
                     /locus_tag="O2458_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3886280.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15689..16693
                     /locus_tag="O2458_000392"
     CDS             15689..16693
                     /locus_tag="O2458_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCZ3886281.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16724..17230
                     /locus_tag="O2458_000393"
     CDS             16724..17230
                     /locus_tag="O2458_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCZ3886282.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17232..17726)
                     /gene="moaC"
                     /locus_tag="O2458_000394"
     CDS             complement(17232..17726)
                     /gene="moaC"
                     /locus_tag="O2458_000394"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCZ3886283.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            17789..19048
                     /locus_tag="O2458_000395"
     CDS             17789..19048
                     /locus_tag="O2458_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCZ3886284.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19045..19530
                     /gene="mobB"
                     /locus_tag="O2458_000396"
     CDS             19045..19530
                     /gene="mobB"
                     /locus_tag="O2458_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCZ3886285.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19544..19990
                     /locus_tag="O2458_000397"
     CDS             19544..19990
                     /locus_tag="O2458_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCZ3886286.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            19996..20229
                     /gene="moaD"
                     /locus_tag="O2458_000398"
     CDS             19996..20229
                     /gene="moaD"
                     /locus_tag="O2458_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCZ3886287.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20236..20835
                     /gene="mobA"
                     /locus_tag="O2458_000399"
     CDS             20236..20835
                     /gene="mobA"
                     /locus_tag="O2458_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCZ3886288.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20855..21877
                     /gene="moaA"
                     /locus_tag="O2458_000400"
     CDS             20855..21877
                     /gene="moaA"
                     /locus_tag="O2458_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCZ3886289.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(22272..22442)
                     /locus_tag="O2458_000401"
     CDS             complement(22272..22442)
                     /locus_tag="O2458_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886290.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            22568..22918
                     /gene="sarV"
                     /locus_tag="O2458_000402"
     CDS             22568..22918
                     /gene="sarV"
                     /locus_tag="O2458_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCZ3886291.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(23020..24231)
                     /locus_tag="O2458_000403"
     CDS             complement(23020..24231)
                     /locus_tag="O2458_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3886292.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(24224..24664)
                     /locus_tag="O2458_000404"
     CDS             complement(24224..24664)
                     /locus_tag="O2458_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCZ3886293.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            24826..25590
                     /locus_tag="O2458_000405"
     CDS             24826..25590
                     /locus_tag="O2458_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886294.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(25582..25833)
                     /locus_tag="O2458_000406"
     CDS             complement(25582..25833)
                     /locus_tag="O2458_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886295.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            26222..27487
                     /gene="femX"
                     /locus_tag="O2458_000407"
                     /gene_synonym="fmhB"
     CDS             26222..27487
                     /gene="femX"
                     /locus_tag="O2458_000407"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCZ3886296.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            27604..30771
                     /locus_tag="O2458_000408"
     CDS             27604..30771
                     /locus_tag="O2458_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCZ3886297.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(30958..31275)
                     /gene="mspA"
                     /locus_tag="O2458_000409"
     CDS             complement(30958..31275)
                     /gene="mspA"
                     /locus_tag="O2458_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCZ3886298.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(31520..31684)
                     /locus_tag="O2458_000410"
     CDS             complement(31520..31684)
                     /locus_tag="O2458_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886299.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            31817..32725
                     /locus_tag="O2458_000411"
     CDS             31817..32725
                     /locus_tag="O2458_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCZ3886300.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            32896..33027
                     /locus_tag="O2458_000412"
     CDS             32896..33027
                     /locus_tag="O2458_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886301.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(33144..34007)
                     /locus_tag="O2458_000413"
     CDS             complement(33144..34007)
                     /locus_tag="O2458_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCZ3886302.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(34188..35075)
                     /locus_tag="O2458_000414"
     CDS             complement(34188..35075)
                     /locus_tag="O2458_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3886303.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            35141..35236
                     /locus_tag="O2458_000415"
     CDS             35141..35236
                     /locus_tag="O2458_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886304.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            35262..37397
                     /locus_tag="O2458_000416"
     CDS             35262..37397
                     /locus_tag="O2458_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCZ3886305.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIFTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            37510..38844
                     /locus_tag="O2458_000417"
     CDS             37510..38844
                     /locus_tag="O2458_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCZ3886306.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(39036..39425)
                     /locus_tag="O2458_000418"
     CDS             complement(39036..39425)
                     /locus_tag="O2458_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886307.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            39782..40090
                     /gene="rpsJ"
                     /locus_tag="O2458_000419"
     CDS             39782..40090
                     /gene="rpsJ"
                     /locus_tag="O2458_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCZ3886308.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            40118..40780
                     /gene="rplC"
                     /locus_tag="O2458_000420"
     CDS             40118..40780
                     /gene="rplC"
                     /locus_tag="O2458_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCZ3886309.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            40807..41430
                     /gene="rplD"
                     /locus_tag="O2458_000421"
     CDS             40807..41430
                     /gene="rplD"
                     /locus_tag="O2458_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCZ3886310.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            41430..41705
                     /gene="rplW"
                     /locus_tag="O2458_000422"
     CDS             41430..41705
                     /gene="rplW"
                     /locus_tag="O2458_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCZ3886311.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            41738..42571
                     /gene="rplB"
                     /locus_tag="O2458_000423"
     CDS             41738..42571
                     /gene="rplB"
                     /locus_tag="O2458_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCZ3886312.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            42638..42916
                     /gene="rpsS"
                     /locus_tag="O2458_000424"
     CDS             42638..42916
                     /gene="rpsS"
                     /locus_tag="O2458_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCZ3886313.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            42945..43298
                     /gene="rplV"
                     /locus_tag="O2458_000425"
     CDS             42945..43298
                     /gene="rplV"
                     /locus_tag="O2458_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCZ3886314.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            43322..43975
                     /gene="rpsC"
                     /locus_tag="O2458_000426"
     CDS             43322..43975
                     /gene="rpsC"
                     /locus_tag="O2458_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCZ3886315.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            43978..44412
                     /gene="rplP"
                     /locus_tag="O2458_000427"
     CDS             43978..44412
                     /gene="rplP"
                     /locus_tag="O2458_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCZ3886316.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            44402..44611
                     /gene="rpmC"
                     /locus_tag="O2458_000428"
     CDS             44402..44611
                     /gene="rpmC"
                     /locus_tag="O2458_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCZ3886317.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            44635..44898
                     /gene="rpsQ"
                     /locus_tag="O2458_000429"
     CDS             44635..44898
                     /gene="rpsQ"
                     /locus_tag="O2458_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCZ3886318.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            44930..45298
                     /gene="rplN"
                     /locus_tag="O2458_000430"
     CDS             44930..45298
                     /gene="rplN"
                     /locus_tag="O2458_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCZ3886319.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            45334..45651
                     /gene="rplX"
                     /locus_tag="O2458_000431"
     CDS             45334..45651
                     /gene="rplX"
                     /locus_tag="O2458_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCZ3886320.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            45678..46217
                     /gene="rplE"
                     /locus_tag="O2458_000432"
     CDS             45678..46217
                     /gene="rplE"
                     /locus_tag="O2458_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCZ3886321.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            46240..46425
                     /locus_tag="O2458_000433"
     CDS             46240..46425
                     /locus_tag="O2458_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCZ3886322.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            46457..46855
                     /gene="rpsH"
                     /locus_tag="O2458_000434"
     CDS             46457..46855
                     /gene="rpsH"
                     /locus_tag="O2458_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCZ3886323.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            46880..47416
                     /gene="rplF"
                     /locus_tag="O2458_000435"
     CDS             46880..47416
                     /gene="rplF"
                     /locus_tag="O2458_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCZ3886324.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            47447..47806
                     /gene="rplR"
                     /locus_tag="O2458_000436"
     CDS             47447..47806
                     /gene="rplR"
                     /locus_tag="O2458_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCZ3886325.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            47827..48327
                     /gene="rpsE"
                     /locus_tag="O2458_000437"
     CDS             47827..48327
                     /gene="rpsE"
                     /locus_tag="O2458_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCZ3886326.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            48344..48523
                     /gene="rpmD"
                     /locus_tag="O2458_000438"
     CDS             48344..48523
                     /gene="rpmD"
                     /locus_tag="O2458_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCZ3886327.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            48540..48980
                     /gene="rplO"
                     /locus_tag="O2458_000439"
     CDS             48540..48980
                     /gene="rplO"
                     /locus_tag="O2458_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCZ3886328.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            48980..50272
                     /gene="secY"
                     /locus_tag="O2458_000440"
     CDS             48980..50272
                     /gene="secY"
                     /locus_tag="O2458_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCZ3886329.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            50289..50936
                     /locus_tag="O2458_000441"
     CDS             50289..50936
                     /locus_tag="O2458_000441"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCZ3886330.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            51129..51347
                     /gene="infA"
                     /locus_tag="O2458_000442"
     CDS             51129..51347
                     /gene="infA"
                     /locus_tag="O2458_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCZ3886331.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            51379..51492
                     /gene="rpmJ"
                     /locus_tag="O2458_000443"
     CDS             51379..51492
                     /gene="rpmJ"
                     /locus_tag="O2458_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCZ3886332.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            51515..51880
                     /gene="rpsM"
                     /locus_tag="O2458_000444"
     CDS             51515..51880
                     /gene="rpsM"
                     /locus_tag="O2458_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCZ3886333.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            51904..52293
                     /gene="rpsK"
                     /locus_tag="O2458_000445"
     CDS             51904..52293
                     /gene="rpsK"
                     /locus_tag="O2458_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCZ3886334.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            52368..53312
                     /locus_tag="O2458_000446"
     CDS             52368..53312
                     /locus_tag="O2458_000446"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCZ3886335.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            53329..53697
                     /gene="rplQ"
                     /locus_tag="O2458_000447"
     CDS             53329..53697
                     /gene="rplQ"
                     /locus_tag="O2458_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCZ3886336.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            54235..55044
                     /locus_tag="O2458_000448"
     CDS             54235..55044
                     /locus_tag="O2458_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ3886337.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            55041..55901
                     /locus_tag="O2458_000449"
     CDS             55041..55901
                     /locus_tag="O2458_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ3886338.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            55891..56697
                     /locus_tag="O2458_000450"
     CDS             55891..56697
                     /locus_tag="O2458_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ3886339.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            56702..57505
                     /gene="truA"
                     /locus_tag="O2458_000451"
     CDS             56702..57505
                     /gene="truA"
                     /locus_tag="O2458_000451"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCZ3886340.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            57745..58182
                     /gene="rplM"
                     /locus_tag="O2458_000452"
     CDS             57745..58182
                     /gene="rplM"
                     /locus_tag="O2458_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCZ3886341.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            58196..58594
                     /gene="rpsI"
                     /locus_tag="O2458_000453"
     CDS             58196..58594
                     /gene="rpsI"
                     /locus_tag="O2458_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCZ3886342.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            59139..60236
                     /locus_tag="O2458_000454"
     CDS             59139..60236
                     /locus_tag="O2458_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCZ3886343.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            60233..60526
                     /locus_tag="O2458_000455"
     CDS             60233..60526
                     /locus_tag="O2458_000455"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886344.1"
                     /translation="MKHPKKGVLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQ
                     YNVSIDKKSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            60587..61330
                     /locus_tag="O2458_000456"
     CDS             60587..61330
                     /locus_tag="O2458_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886345.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            61331..>61668
                     /locus_tag="O2458_000457"
     CDS             61331..>61668
                     /locus_tag="O2458_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ3886346.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEI"
BASE COUNT        22050 a         9193 c        11566 g        18859 t
ORIGIN      
        1 ttataacata gttggataga gtttcgattt aataaattac atgtgaacct tgctacaaca
       61 agatgtgcat cagaggagtg gtttaataat gagtaaaatt aatgacatta atgatttagt
      121 caacgcaaca tttcaagtta agaagttttt cagagataca aaaaagaagt tcaatttgaa
      181 ctatgaagaa atttatattt taaatcatat tttaagaagt gagtctaacg aaatctcatc
      241 taaagagatt gctaagtgct cagagttcaa accttactat ttaactaaag ctttacaaaa
      301 gctaaaagat ttaaaattgt tatcaaagaa aagaagttta caagacgaaa gaacagttat
      361 tgtttatgtt acagatacac aaaaagcaaa tattcaaaaa ctgatttcag aattagaaga
      421 atacattaaa aattaaatca aggttaattg cgtttaataa cattgaacga taacaattta
      481 ttaaaacgaa gttatttatt cagcattggg acataaaatt aacttaaaat ttaaatattg
      541 aagatgcttt aattaaagtt aaagactagc cataccttat ttcagcttat taagcttgaa
      601 acaaggtaca ctagtctttt tattttaata ttttcttaga aaatcaagtt tacgatcata
      661 aatattttct gcgatatagc tttggatggt tccaagtatt ttctctataa tttgtgtgcg
      721 ataagcaaaa attctaactg caaaaccatg tgtaggcaat tgagaaatag caacacgaca
      781 atcgaatgta ttgctataag aactaatggt ttcataaact gaatcgatta gtttttggtt
      841 aacatcttcg tgtatgaagt aagcagagcc ataatgagag taatgttcca tataacctat
      901 ttcattgatc gattgtttat taggattcaa taataaatta tcatatgtga ctaactcatc
      961 ttcaatataa atttcattta ttaaatgcat atattgatac ttgaaggctt cgccagtttt
     1021 tgaataacca ggagttaaaa tatcagtata aaataatgaa ctagaattat tgagattgaa
     1081 cgtattgtgt tgataaaatt tagcattttc atatgcgatg attggatcag cgacatattc
     1141 taaatatgcg ttatctttca aattaaaagt ttgatactgc tcaacatgat tggaaggtgt
     1201 cttgtatatt tttgttgcac cttgagatgt caatgtcact ttagcgttat cttcgacatt
     1261 cacattcata cggtaacgat ctccatctaa atagccacca cctacattta ctatataaaa
     1321 tgtaggaatg gtagattgat ttagataaac tggacgtatc actttaagtg ctttttcaaa
     1381 gaaaatatct cttgatactg atcgattgcc atcgaaaaac actgttaaat caagttgccc
     1441 agtccattgt tgttcatcca ttatgataat cctttgagta aagtgtcgcg ttcaatccaa
     1501 tcgataactt catccaaacc ttcgtcggtt tttaagttag taaaagtaaa tggacgttta
     1561 ccacgaaata ctttagtatc ttcagccatt tgttctaatg atgcacctac atagggagct
     1621 aaatcagttt tgttaattac aaagaaatct gacttaatca taccttgacc acctttacgt
     1681 ggaatctttt caccttgagc aacatcgata atatatattg aaaagtcaac aagttctgga
     1741 ctaaatgttg ctgctaagtt atcgccacca gattctatga aaattagttc tatatcgtca
     1801 tgacgttcta ataattcgtc tattgctgca aagttcatag atgcatcttc acgaatcgca
     1861 gtatgaggac atccaccagt ttcaacacca atgatacgac tttcaggtag aactcctgaa
     1921 tttactaata tcttttcgtc ttcttttgta tatatatcat ttgtaataac gccgatactc
     1981 atttcttttg aaagacgttt tacaactttt tcaattaatt gtgttttacc tgcacctaca
     2041 ggaccaccaa taccaatttt aatcggattt gccacaatta taacctccta tgatatgaaa
     2101 attctaacat tgacgttctc atgcgccatt tgatttagtt ctaaaccagg cgctgtcatg
     2161 ccaaaatctg cttcttttaa ttcgaaaatc tgctttcttg ttccttctat ataaggaatc
     2221 atgtgagtaa ctatcttttg accagcagtt tgtccaagtg gaatagcacg aacagcattt
     2281 tgagttaaac ttgaaacatt ttgatataaa tagtaatcaa taatcgtttc aatatctaca
     2341 cctaaatgat ggcctagcat agtaaaacaa atagctggat ttaactttgc tttcttatct
     2401 tgcatttgtt gatgatacca agcaatccat gggctattat aaagttctaa agccaattta
     2461 accatgcgag tccccatttg ttttgcacca acacgtgttt ctttaggtaa gttttgaaca
     2521 aacatcagtt tatctatgtg taatactttt tgtgtatcat cattttccaa tgcatcataa
     2581 actaaacgca tggctaaacc atcagaatag gtaagttgct cttgtaaaaa catttttaac
     2641 caagcaataa aagtatgatc gtcatgaatt atatttcgtt gaatatatgt ttcaagacca
     2701 aatgaatgac tgaaagcacc tgttggaaac tgtgaatcac agaactgaaa taatcttaag
     2761 tgtgtatgat caatcatgag aatgccctat atgtctgaaa gccttattaa ctttacggtc
     2821 ttctcgaaca tatgggatgc ctaaactttt taataaatct tcaactaaat aatcatattg
     2881 tactagcatt tcagtctctg taaattgtgc tggcaaatga cgatttccta attgatgggc
     2941 tatatctccc atttcttgca atgttcttgg ttgaatcact aaaagatctt ctgaattaac
     3001 atccacaata atcatattat ggtcatctgc gtataaaata tctccatatt gtaagtcaat
     3061 aggttgtttt aaacgaatgc ctatttcagt gccatggtct gtaacgactc tttgaatacg
     3121 tttaacaaga tctgaatttt caaggtatac tttttcgacg tgcttttgtt tttctgaatt
     3181 tgacaaattg gcaatattgc cttggatttc ttcaacaatc attctatgtt cctcctagaa
     3241 taagaagtat ctttgagtta atggtaactc agttgctgca ttacttgtaa ttttttctcc
     3301 atctacatat acttcatatg tttgtggatc aacgtctaat ttaggtgttg cactgttatt
     3361 tttcatatct gctttagata attgtctaat atttttaact ggacgcacca tgcgttttaa
     3421 atttaatgca cgattgatac cattttcata agcagtttta gacacgaatg tcattgacgt
     3481 acttgtaagg tttccgccgt attgaccata cattttacgg tacttcatcg gttcagatgt
     3541 aggtatagaa ccatttgcat cgccatttac ggcagagtta attaatccgc cctttacaac
     3601 taattcaggt ttaaccccaa agaaaattgg gtcccataag acaatgtcag ctagtttgcc
     3661 cggctcgata gatcctacat attcagaaat accatgtgta attgctgggt taattgtata
     3721 tttagcgata taacgtttga tgcgattatt atcattatgt tcaaaatcac catctaaagg
     3781 accacgttgt tctttcatgc gatgtgctac ttgccatgtt cgtgtaatta cttcacctac
     3841 acggcccatt gcttgtgaat cggaactaat catactgaat acacccatat cttgcagaac
     3901 atcttctgct gcaatcgttt ctttacgaat acgtgaatct gcgaatgcga tatcttcagg
     3961 aatagccgca tttaaatggt gagtaatcat taccatatct aaatgttcat ctacagtatt
     4021 atgtgtataa ggcaaagttg gatttgtaga tgaaggtaaa atatttgaaa atgcagcgga
     4081 tttaattaaa tcaggcgcat gaccgccacc agcaccttca gtatggtaca tatgaagtac
     4141 acggtcttta acagcagcca ttgtgtcttc cataaatcct gcttcattta aagtatctgc
     4201 atgtaatgca atttgaacat caaattcatc agcaacatct aatgcatgac tcaaagcaga
     4261 tggtgttgca ccccagtctt catgtacttt taatccaatt gctccggcat tgatttgttc
     4321 aatgagtgca gttggatttg ttgcttgtcc tttacctgta aaaccgacat taatcggtaa
     4381 accttcggca gcttctaaca ttctatgaat atgccatgga cctggagtta cagttgttgc
     4441 tttagaacct tctgaagcac cagtaccacc accaatatga gtcgtaatac cactttctaa
     4501 tgcgacctct gcttgttcag gattaataaa atgaacatga gtatcaatac caccagcagt
     4561 gacgatttta ccttcagcgg caatgatatc tgttgttgaa cctataataa tgtcgacatt
     4621 atccattata tctgggttgc cggcattacc tatggcgaaa atataaccat ttttaatgcc
     4681 tatatcagct ttaaccactt tatcgtaatc gataataacg gcattagaaa tgacaaggtc
     4741 tgcaacgttc acgtcatcac gtgttacacg aggattttgc gccataccgt ctctaataga
     4801 tttaccacca ccaaaagtag cttcttcacc ataaaccgca tagtcttttt ctatttgagc
     4861 aaatagattc gtatcaccta aacgaatgga atctccaaca gttggaccgt ataagctcgt
     4921 atattgattt tgcgtcattt taaagctcat gatctttttc ctcctttttt attcacgttt
     4981 tcagcaccgt tatctccgaa tacacctgca tattcatcat tttcatcagt tgggcgatag
     5041 acacgtgact catcgatagg accattgacc ataccacgaa aaccaaaaat tttacgtttg
     5101 ccagcatatt caactaattg aacttctttt ttatccccag gttcaaatcg aacagctgct
     5161 ccagctggaa tatctaaatg ttttccatat gccatttcac gttcgaaatc taatgctgca
     5221 tttgcttcat aaaaatgaaa atgtgagccc acttgaattg gtcgatctcc tgtattttca
     5281 acttcgataa ctgtttcagg atgatggtta ttaatttcaa cctctgtact ttttgtaata
     5341 atttctcctg gtatcatttg actgcctcct ttaaacaata gggtgatgta ctgtgattaa
     5401 cttagtacca tcggggaacg tagcctcgat ttcgatatct gtaatcatgt gttcgacacc
     5461 atccatgaca tcttctttgt ttagaatttg tctaccataa ctcattaact ctgcaacggt
     5521 cttaccatcg cgtgcacctt ctaataattc atcgctgatt aaagctaatg cctcaggatg
     5581 atttagtttc aaaccacgtg ctttacgacg acgtgcaact tccgccgcca ctacaatcat
     5641 taatttgtct tgctctcgtt gtgtaaaatg caaattaaaa cccccaattt catattagat
     5701 acaatttaca aaatttatat taatcctaat tgttgtgata aacaagtaat atacaaagtt
     5761 caatgtgtaa ttagaaaatt atatttttag catatccgat attgaagcaa acaatctaat
     5821 cgaaaacaaa tagtggaata tatttatgta aaaaccaaaa tagtttttaa tataactttt
     5881 catagaatag tagtatatta atgagtaatg attcaaagga aaggtgaaag atttgaagat
     5941 aatagatgtg cttttgaaaa atatatctca ggttgtgtta attagtaata aatggacagg
     6001 attatttatc ttaataggat tatttgtagc cgattggaca attggattag cggctattgt
     6061 aggtagcatc atcgcctata cttttgcgcg ttttataaat tatagtgagg cagagattaa
     6121 tgatgggtta gctggattta atccagtgct aactgccatt gcgttaacaa tctttttaga
     6181 taagtcagga ttagatattg ttataacaat gatagcaact ttattaacgt taccagttgc
     6241 tgctgcagtg agagaagttt taagaccata taaagttccg atgctgacga tgccttttgt
     6301 cattgtgact tggtttacaa ttttactttc aggacaggtt aaatttgtag atacatcgtt
     6361 aaagttaatg cctcaaaaca ttgaaacggt taattttagc aacaatgata gaatacattt
     6421 cattcagtca ttatttgaag gattcagtca agtatttatc gaagcgagtg taattggtgg
     6481 cgtatgtatt ttaatcggca tattgatagc atcaagaaaa gcaacactct tagctgttat
     6541 agctagtttg ttaagcttta tcattgtagc tctattaggt ggtaattatg atgatattaa
     6601 tcagggatta ttcggttata actttgtatt aatggcaatc gcactaggat atacatttaa
     6661 aacagcgatt aacccttata tttcgacttt tttaggtgtg ttattaacag tagtggtgca
     6721 actaggtaca acaacattgc ttgaaccgtt tggcttacct gcattaacat tgccatttat
     6781 tatcgtgaca tggattttat tatttgctgg tattaaacat gacaaagtag atgcttgata
     6841 gttaaatcaa acctaatatt gtttgaatat caccttaaac tatacagcga attgtatagt
     6901 ttaaggtgta tttttatgga taaaattaag tgcatactta atcgaattat cagttttctt
     6961 ttaattgttc acagcacgtt cctttttatt tttaaagtcg agtgttaaac taggaataaa
     7021 taatttatat tgtgtgttgt gtggggtgac taatatgaat gatatggata attccttttt
     7081 aataacaacg gaaattcaaa gaaaatggat tgaaaaattc aaagtaatta gagatacatt
     7141 taaggctaaa gctgaatata atgatcaaca tagccaattt ccatataaaa atattgaatg
     7201 gttaattaaa gaaggttatg gaaaattaac gttaccaaaa gcatatggtg gtgaaggtgc
     7261 gaccatagaa gacatggtta ttttgcaatc atttttaggc gaacttgatg gtgccacagc
     7321 attatctatt ggttggcatg tgagtgtcgt aggacaaatt tatgaacaga aattatggtc
     7381 tcaagatatg ttggagcaat ttgctgttga aattaataat ggtgcattag ttaatagagc
     7441 agttagtgaa gctgaaatgg gtagtccaac aagaggggga agaccaagta cacatgctgt
     7501 taaagctgat gatgggtata ttttaaatgg tgtgaagaca tatacatcaa tgagtaaagc
     7561 actaacacat attattgttg ctgcttatat agaagaatta gaaagtgttg gttttttctt
     7621 agtagacagg aatttgcctg gtgttgaaat tgccgataat tgggacgtat taggtatgcg
     7681 agcgacagaa agtcatgatt taatattaaa tgatgtaaaa gtaccattaa aacatttagt
     7741 ggaaacagag aaaagtaaag cgccaaatgg ttggatattg catataccaa gctgttattt
     7801 aggtatagca caagctgcta gaaattatgc tgtagacttt gcaatccaac atagccctaa
     7861 tagtattgaa ggaacgattg caacattgcc aactgtgcaa caaaatttag ggaaaatgga
     7921 aacgctattg ttatgtgcga gacaattttt atggagtaca gcaaaagggt atcaacaata
     7981 taaagatgac agtcagataa gaaatccaac aagtgcgagt aaagtgatgg taatgaatca
     8041 agggcttgaa gtgattgatt tagcgatgag aatagttggt gctaaaagct tagaaatgaa
     8101 tcgaccatta caacgttact atcgtgacat gcgtgcagga ttacacaatc cacctatgga
     8161 agatgcagca tatacaaata ttgctaagag tattacagat acattttaaa ttttaaaagg
     8221 taaatcgatc gttgattggc ataaagagat gaaatttgac gttcggattt accctttttt
     8281 tgtggggatt tatagggatg ttaagatatt ttgttaaatt ttttgcgaaa ataaaaaata
     8341 aataacacta gctattgtaa atgtatatgc aatcttgtat aatttttgaa tgattgataa
     8401 tcattttcaa taggaggaaa ttatgaaaaa actattatta ccattaataa ttatgttatt
     8461 agtgttagct gcgtgtggga accaaggtga aaaaaataac aaagctgaaa ctaaatctta
     8521 taaaatggac gatggcaaaa cggtagatat tccgaaagac cctaaacgca ttgcagtagt
     8581 tgcgccaaca tatgctggtg gacttaaaaa attaggtgca aacattgtag ctgtaaatca
     8641 acaagtcgat caaagcaaag tattaaaaga taaatttaaa ggtgttacaa aaattggtga
     8701 tggcgatgta gaaaaagttg ctaaagaaaa gccagattta attattgtat actctactga
     8761 caaagatatt aaaaaatatc aaaaagtagc accaacagta gttgttgact ataataagca
     8821 taaatattta gaacaacaag aaatgttagg gaaaattgtt ggtaaagaag ataaagtaaa
     8881 agcttggaag aaagattggg aagaaacaac tgctaaagac ggtaaagaaa ttaaaaaagc
     8941 aattggacaa gatgcaacag tgtcattgtt tgatgaattt gataaaaaat tatacactta
     9001 cggcgataac tggggtcgtg gtggagaagt attatatcaa gcatttggtt tgaaaatgca
     9061 accagaacaa caaaagttaa ctgcaaaagc aggttgggct gaagtgaaac aagaagaaat
     9121 tgaaaaatat gctggtgatt acattgtgag tacaagtgaa ggtaaaccta caccaggata
     9181 cgaatcaaca aacatgtgga agaatttgaa agctactaaa gaaggacata ttgttaaagt
     9241 tgatgctggt acatactggt acaacgatcc ttatacatta gatttcatgc gtaaagattt
     9301 aaaagaaaaa ttaattaaag ctgcaaaata attcagctat ataagttagt gaaatgagag
     9361 tctgaaacat atcaatcttt tgatattgta ttaggctctt atttttatag ctagaaagtt
     9421 agatatttgt attttttaaa taataagtgc cgttgttatc gttcaattta attaatgata
     9481 gattagtatt attatagcta aagtagtata cctgagaaaa tagctcaatg tatctcttta
     9541 ttaataagtt atatcataat tattttagtg catactttat ggaagggata tcagggaatg
     9601 gctttcaatt aaagaagagg tttaaaagga ttacaacaga atgttatgat tttgtagaaa
     9661 gatatataac aacgttttat aaaaacataa tattgttaat ggaaaatgaa atgtaagggg
     9721 gatttcgagt gactaagaaa gtttatttta accacgatgg tggtgtagat gatttagtat
     9781 ctctattttt attattacaa atggaaaacg ttcaattgat aggggtcagt acaattggtg
     9841 ctgattgtta tttagagcca tctttgagcg catcagtaaa aattattaat cgtttttcaa
     9901 atgaagatat tcaagttgcg ccatcatatg aacgaggaaa aaatccattt cctaaagaat
     9961 ggcgtatgca tgcctttttt atggacgcat tgccaatttt aaatgagcca gtcaaacatg
    10021 ttgcttcaaa tgtgagcgac aaagaagcct ttgaagacat tattcaaact ttaaagagac
    10081 aatcagaaaa agtaacatta ttatttacag gcccgcttac agatttagca aaagcactac
    10141 aaaaagattc atctatcgtt cagtatatag aaaaattagt ttggatgggt ggcacctttt
    10201 taccaaaagg aaatgttgaa gaacctgagc atgatggttc tgcagaatgg aatgcatatt
    10261 gggatccaga agcggttaaa attgtttttg atagcgatat agagattgat atggttgctt
    10321 tagaaagtac gaatcaagta ccgctaacgt tagatgttag acaaagatgg gcgaatgaac
    10381 gccaatatac gggcattgat tttttaggag taagttatgc agcggtacca ccattaacac
    10441 actttataac aaattctact tactttttat gggatgtttt aacgactgct tatattggta
    10501 acaaggactt ggttcattca attgagaaaa aagtcgatgt aataagttat ggaccaagtc
    10561 aaggtaagac atttgagtgt aaagatgggc gcaaaattaa tgtcataaat catgtagata
    10621 acaacgcatt ttttgattat ataactgcac ttgctaaaaa agtaaattaa cagctgtgta
    10681 gaataattaa ggttttaatt tatatagaac aacttattgt aaacttttca tttcttaaag
    10741 tttacaatgg tgctataata atggtcatga aatacgaaag gaagtaaaaa atgacaacaa
    10801 aacagttagt atatacagct ttaatgacag cgattatcgc tattttagga ttggtaccgg
    10861 taattccact accattttct tcagtaccaa ttgtacttca aaacattggt attttcttag
    10921 caggtgtgat tttaggacgt aaatatggca cattaagtgt tatcgtcttt ttattattag
    10981 tagttgctgg cttgccattg ttatcaggtg gtcgcggtgg catcggtgta ttcgcaggtc
    11041 cttcagcagg gtttttacta ttatatccag ttgtagcatt catgattggg gcgattcgag
    11101 atagattcat caatgaaatt aatttctgga ttttattcgt tggtatttta gtttttggtg
    11161 ttatagcatt agatgttatt ggtacattga ttatgggcat gattattaac ataccattta
    11221 cgaaagctat ttcaatttca ttagcttatt tgcctggtga tatattaaaa gcaattgtag
    11281 caagtttgat tggtacagct ttacttaatc actcgcagtt tcgtcaaatt atgggaataa
    11341 aataatcata tttaagatag taaagtaatt gaataagttg ctttgaaatt tataaaagtg
    11401 aaaggagtag gtgtcaatgg ctagtataag tatgtcagat atatattgta acggcactat
    11461 atttgaaaat gacgacgagc agttgattta tttaacgcct tcttttccac aacgatacac
    11521 aagtaacaca tggatatata aaaagacgcc tacccaagag cgatggctga aagacttaga
    11581 acgtcaacat caattacata caaatcaagg ttcaaatcat tatgcgttta gtttcccgga
    11641 aaatgaacaa cttgataatc attggatggc tatgtttaaa gatatgaatt ttgaactagg
    11701 tattatggaa ttgtatgcca tagaaagtga tgcgcttgcc aatttgccgc gtaactctga
    11761 cgttgaaatt gccatcgttg acgagtcgca tatagatgcc tatttaaaag ttgcatatca
    11821 gtttagtttg ccatttggaa aagactatgc agatgcacat gaagaaatgg taagggaaca
    11881 ttatcaaaaa gatgtgatta aacgcttagt agcttattta aataatgaac ctattggcgt
    11941 tgtagatgtc attgaaagtg aaaattacat tgaattagat ggatttggtg tattagaaca
    12001 atttcggcac caaggaattg gatctacaat tcaatcgttg ataggtgaat acgccatatc
    12061 aaaaaatcac aaaccaatca tattagttgc agatggtgaa gatacagcaa aagatatgta
    12121 tgcaaagcaa ggttatgtct atcaatcgtt ttgttatcaa atattaaaag aagatattgg
    12181 aaattaacga ttgatgatag gtttgtatta gaataaaaaa gcagtaagct ggtttcgagt
    12241 agaaatcaac ttactgcttt ttaaattgtt ttgagctact tatacttata aaaatagtgc
    12301 gtttaaattg ttgattcatg tagaatatcg ttcattatga cacactataa tgaatatgtt
    12361 attgttcaga atcaatgata cgttctggat gactgtatat attaaagcca ccatttcgaa
    12421 taaatccaac tgccgtaata tttaggtcat tagctaaggt tacagcaagc gttgtcggag
    12481 ctgatttaga taaaatgacg ccaacaccaa tttttgcggc tttaattaaa atttctgatg
    12541 aaatacgtcc actaaaaatt aatactttat ctcggacagt aatatgtcgc tgaatacaaa
    12601 atccatataa tttatctaga gcgttatgtc taccaatgtc ttgtcgatgt acaaaaaatg
    12661 tcaaaccatc gcttatagca gcattatgta agccacctgt ttcttggtaa atatgacttg
    12721 cactttgtaa tcgagtcatc atgttaataa tttgcattgg agttaaagtg attttagaca
    12781 tagatgtttt agcgatagca gcatcatttt gaaaataaaa ctcacgactc tttccgcaac
    12841 aagatgcaat cattcgtttt gtggaatatt gaaagcgatc gcctaaatct ttattaagtt
    12901 caacatgggc aaaaccttta ctatcatcaa tcagtacaga ttttaattca tctcgcttta
    12961 aaatggcacc ttccgaagcc agaaatccaa tgactaactc ctcaaggttt gttggactgc
    13021 atataacagt cgcaaattct tcaccattca ccataattgt aagtggaaat tctgtcacat
    13081 attgatctgt tgtattgaat aattttccat cttcatatct aacaattggt tgacctaaag
    13141 atacatcttt gttcattatc taaccccttt aattagctta aactttattt taaagcaatt
    13201 tgcttaaaat tttaacatat ttgcttaagt ttgaaatttg attgataaaa attaatagcg
    13261 agcaatctgt ttgatttaaa ttgaattcga gaatatacat actagggcat caattaataa
    13321 atatcaatct tatgcaaatt tgacaattgt ttgaatcaat atataaacag gcaacggttc
    13381 ttttcaaata taatagtaag tgtataatga aaatgtaaat attattaaaa atgggggttc
    13441 actcaatgaa attgaaacgt ttatttgctg ttgtgattgc aatgctttta gtattagctg
    13501 gttgctctaa ttctaacgat aataatgaaa gtaaaaaaga tgacgcagac aatggtaaga
    13561 aacaagagat tcaagttgca gcggcagcaa gtttaacaga tgtaaccaag aaattagctt
    13621 cagaatttaa aaaagagcat aaaaatgctg atattaaatt taactatggt ggatcagggg
    13681 cattaagaaa acaaattgaa tcaggcgcac ctgttgacgt atttatgtct gcaaatacta
    13741 aagatgtaga tgcattaaaa gacaagaata aagcgcatga tacatataaa tatgcgaaaa
    13801 atagtctagt attaattggt gataaagatt caaattacac ttcagtaaaa gacttaaaag
    13861 acaatgataa attagcatta ggtgaagtga aaactgtacc agcaggaaaa tatgcgaaac
    13921 agtatttaga taacaataac ttatttaaag aagtcgaaag taaaatcgtt tatgctaaag
    13981 atgtaaaaca agtattaaat tatgttgaaa agggtaatgc gaaacaaggt tttgtgtata
    14041 aaactgactt atataaacaa aataaaaaaa ttgatactgt aaaagtaatt aaagaagtag
    14101 aacttaagaa gccaatcaca tacgaagctg gtgctacatc agatagtaaa ttagcaaaag
    14161 agtggatgga attcttaaaa tcagataaag ctaaagaaat actaaaagaa taccactttg
    14221 cagcataagg agttgtaatc catgcctgac ttaacacctt tttggatatc aatacgagtt
    14281 gctgtaatca gtacgattat tgtaacggtt ttaggtattt ttatatctaa atggttgtat
    14341 cgtcgtaagg gttcgtgggt taaattattg gaaagtttat tgatattacc tattgttttg
    14401 ccgccaacgg tattaggttt tattctatta atcatcttct cgccaagagg accaatcggt
    14461 caattctttg cgaatgtact acatttacct gtagtgttca ctttgacagg tgctgtgata
    14521 gcatctgtca ttgttagttt tccactaatg tatcaacata ctgtgcaagg cttcagaggt
    14581 atagacacga aaatgattaa tacagctaga acgatgggag caagtgaaac gaaaattttc
    14641 ctcaaattaa ttttaccatt agctaaacgc tctattttag caggtataat gatgagtttt
    14701 gctcgtgcat taggtgagtt tggtgctaca ttaatggttg caggatatat tccaaataaa
    14761 acgaatacac tacctttaga aatatacttc ttagtggaac aaggtagaga aaatgaagcg
    14821 tggttatggg tattagtgct agtcgcattc tctattgtgg ttatatctac aattaattta
    14881 ttgaataaag ataaatataa ggaggtcgac tagatgctta aaatcaatgt gaaatatcaa
    14941 ttaaagaaca ctttaattcg catcaatata gatgatactg aaccaaaaat ttatgcagtt
    15001 cgtggtccat ctggcattgg taaaactact gttttaaata tgattgccgg attacgtaaa
    15061 gcagatgaag ctattatcga agtgaatggg caattactta ctgatacggc aaaaaacgtg
    15121 aatgttaaaa ttcaacaacg acgtattgga tatctgtttc aagactacca attgtttcct
    15181 aatatgacgg tctataaaaa tattactttt atggctgaac catctgaaca catcgatcaa
    15241 ttaattcaaa ctttaaacat tgatcatttg atgaaacaat atcctatgac attgtcaggt
    15301 ggagaggcac aacgtgtagc acttgcacgt gcacttagca cgaaaccaga tttaatttta
    15361 ttagatgaac ctttttctag tttggatgat actacaaaag atgagagtat tacattagtt
    15421 aaacgtattt tcaacgaatg gcaaatacca atcatatttg tgacacattc aaactatgaa
    15481 gcagaacaaa tggctcatga aattattaca attgggtaat catttatttg ccattaaaga
    15541 gtttagaacg tatttaaaat tgtagaagtg aatgcttcta tcagcatttt aatgatgttt
    15601 taaactcttt tttaggggca gtttttttga gagacattga cgcgcgtcat ataatgaaag
    15661 taatgataaa aagaaaggat aacttaatgt gagtcaagaa cgttattcaa ggcaaatttt
    15721 atttaaacaa ataggtgaaa taggtcaaag caaaataaat caaaaatgtg cgttgattat
    15781 tggtatgggc gcattaggta cacatgtggc cgaaggactt gttagagcag gcattgccaa
    15841 actaatcatt gttgatagag attatattga atttagtaat ttacaaagac aaacattgtt
    15901 tactgaagaa gatgctttga aaatgatgcc taaggtggtt gcagctaaaa agcatttgct
    15961 agcgttacgt agtgatgttg atattgatga ttatattgcc catgtggatt attatttttt
    16021 ggaaacacat ggacaggacg ttgacgttat tattgatgca accgataact ttgaaacacg
    16081 acaactgatt aatgattttg catataaata tcgtatacct tggatttatg gtggtgttgt
    16141 acagagtaca tatacagaag ctgcatttat acctggtaaa acaccttgct ttaactgttt
    16201 ggtaccacaa ttgccagcat taaatttaac atgtgataca gtaggggtca ttcaacctgc
    16261 cgtgacgatg gcaacaagtt tacaattaag agatgcgatg aaagtattaa cggaacaacc
    16321 aattgacaca aaaataactt atggcgatat ttgggaaggt agtcattatt catttggttt
    16381 cagtaaaatg caacgttcag actgtacaac ttgtggagat gtaccaagtt atccgtattt
    16441 aaacaagaat gaacaacgtt atgcaacatt gtgtggtaga gacactgtac agtatgaaaa
    16501 tgcatcaatt acacacgaca ttcttgttca atttttaaaa caacatcagt taaattatcg
    16561 cagtaattcg tatatggtta tgtttgaatt taaaggacac cgcattgttg cttttaaagg
    16621 tggaaggttt ttaatacatg gcatgacacg cacatcagat gccacacatc taatgaattt
    16681 attgtttgga taaaaaaaga taagacaaaa ggagtgtaat attatgggcg aacatcaaaa
    16741 cgttaaattg aatcgtacag ttaaagcagc cgtactaacg gtatcagata ctagagactt
    16801 tgatacagat aaaggtggtc aatgcgtgcg ccaactatta caagcagatg acgttgaagt
    16861 gagtgacgca cattatacaa ttgtgaaaga tgaaaaagta gccatcacga cgcaggtgaa
    16921 gaagtggtta gaagaagata ttgatgtcat cattacgact ggtggaacag gtattgcaca
    16981 acgtgatgtg acgattgaag cagtaaaacc acttttaact aaagagatag atggctttgg
    17041 ggaattgttt agatatttga gttatgttga agatgttggc acgcgtgcat tattgtctcg
    17101 tgctgtagca ggtacagtta ataataaatt gatattttcg attccaggat caacaggcgc
    17161 agttaaatta gcattagaaa agctcattaa accagaatta aatcatctga ttcatgagct
    17221 tacaaaataa tttattgatt tgattggcgt tgaaaatctc cagatttacc gccagacttg
    17281 ctttcaaggt aggtttcgcc aataatcata cctttatcaa ctgctttcgt catgtcgtaa
    17341 atggttaaag ccgttgctga tgcagcggtt aaagcttcca tttcaacacc ggttttgcca
    17401 gttgtagaga cagttgtttg aatgtttaaa gtataaaggg gtgcatttgt ttcatcccag
    17461 ctgaagtgaa catctatgcc agtcaatggt aatggatggc acatcggaat aagtgttgat
    17521 gtatttttgg cagccataat accagcgatt tgagcagtgt tcaatacatt gcctttggta
    17581 ttggtgttat tagaaatctg cttaaaaata gtttcattta cagtaatgct tgaatgagcg
    17641 acagcagttc tttttgtaat ttgtttgtct gatacatcga ccattttggc gtggccttgt
    17701 tgattaatat gagtaaactc agtcatttta cccctcctag tgcatctagt atatcatgaa
    17761 aaaataaaag ttttggagat gatttttaat ggtagtagaa aaaagaaacc caatcccagt
    17821 taaagaagca attcaacgta tcgttaatca gcagagttca atgccggcaa ttacggtagc
    17881 acttgaaaaa agtctaaatc atatcttagc agaagatatt gtagctactt atgatatacc
    17941 aaggtttgat aaatcacctt atgatggttt tgcaattcgc agtgttgatt cacaaggggc
    18001 aagtggtcag aatcgcattg agtttaaagt gattgatcat attggtgcag gttcagtttc
    18061 tgataaatta gttggggatc acgaagcggt gcgtattatg actggagcac aaatacctaa
    18121 tggcgcagat gctgttgtta tgtttgaaca aacgattgaa ctagaagata catttacaat
    18181 tcgtaaacca ttttcaaaaa atgaaaatat atctttaaaa ggtgaagaaa caaagacagg
    18241 cgatgttgtt ctaaaaaaag gacaagtaat taatccaggg gctatcgcgg tccttgcaac
    18301 atatggctat gcagaggtta aagttattaa gcaaccgagt gtcgctgtta ttgcaacagg
    18361 aagcgaatta ttagatgtta atgatgtatt agaagatgga aaaattcgta actctaatgg
    18421 cccaatgatt cgtgccttag cagaaaaatt aggtcttgaa gttggtattt acaaaacaca
    18481 aaaagatgat ttagatagtg gcatccaagt cgttaaagaa gctatggaaa aacatgatat
    18541 cgttattaca acgggcggag tttctgttgg agattttgac tatttacctg agatttataa
    18601 ggctgtaaag gcggaagtgt tatttaataa agtagcaatg cgtcctggta gcgtaacaac
    18661 ggttgcattt gtagatggaa agtatttgtt tggattatct ggaaatccat cagcttgttt
    18721 tacaggattt gaactatttg tgaaaccagc tgttaaacat atgtgtggcg cactagaagt
    18781 cttcccgcaa ataattaaag caacattaat ggaagatttt accaaggcaa acccattcac
    18841 acgatttata cgtgctaaag caacgttaac aagtgctgga gctactgtag taccttcagg
    18901 attcaataaa tcaggtgcgg ttgtagcgat tgcacatgct aactgtatgg tcatgttacc
    18961 aggagggtca cgtggtttta aagcggggca tacagtagat attatattga ctgaatctga
    19021 cgctgctgaa gaggaacttc ttttatgatt ttacaaattg taggttacaa aaagtctggt
    19081 aagacaacat tgatgaggca tattgtctct ttcttaaagt cacatggtta tacagttgct
    19141 actattaaac atcatgggca tggtaaggaa gatattcaat tacaggattc agacgtcgat
    19201 cacatgaagc attttgaagc gggggcagat caaagtattg tacaaggttt tcaatatcag
    19261 caaactgtaa cacgtgtaga taatcaaaat cttactcaaa ttattgaaaa atctgttaca
    19321 attgacacca atatcgtatt agttgaaggc tttaaaaatg ctgattttga aaaagtcgta
    19381 gtctatcgaa atgaagaaga gttgcaagta ttacaacaat tgtcgaatgt ttgttatagc
    19441 attaatgtaa gggagcatga agattttaca gcatttgagc aatggttatt aaataaaatt
    19501 aaaaatgatt gtgatacaca attaacatag aggattgaaa tgaatgaaac aatttgaaat
    19561 cgtgacagaa ccgatacaaa cagaacaata tcgtgaattc actataaatg aatatcaagg
    19621 tgcagtagtt gtttttaccg gtcatgttcg cgaatggact aaaggcgtca aaacggaata
    19681 tttagaatat gaagcgtata ttccaatggc tgaaaagaaa ttggcacaaa ttggagatga
    19741 aataaatgaa aaatggcctg gaacgataac gagtattgtt catagaatag ggccattaca
    19801 aatttcagat atcgctgtat taattgcggt ttcttcaccg catcgtaaag atgcctatcg
    19861 agcaaatgaa tatgcaattg agcgtataaa agaaattgtt ccgatttgga aaaaagaaat
    19921 ttgggaagat ggttcaaaat ggcaagggca tcaaaaaggg aattatgaag aagcgaagag
    19981 ggaggaataa gagagatgaa ggtactttac ttcgcagaaa ttaaagatat attacaaaaa
    20041 gcacaggaag atattgtgct tgaacaagca ttgactgtac aacaatttga agatttattg
    20101 tttgaacgtt atccgcaaat caataataaa aagtttcaag ttgctgtaaa tgaggaattt
    20161 gtacaaaaat cggatttcat tcaacctaat gatactgttg cattaattcc accggttagt
    20221 ggaggttaag ggagcatgaa agcaataatt cttgcaggtg gtcattcagt gcgatttggt
    20281 aagcccaaag cttttgcgga agtgaacggt gagacctttt atagtagagt aattaagaca
    20341 ttagaatcaa caaatatgtt caatgaaatt attattagta caaatgcgca attggcaacg
    20401 caatttaaat atccaaatgt tgttatagat gatgagaatc ataatgataa aggtccatta
    20461 gcaggaattt atacaatcat gaagcaacat cctgaagaag aattgttttt tgtcgtttct
    20521 gttgatacac caatgattac tggtaaagct gtaagcacgt tgtatcagtt tttagtttct
    20581 catcttattg aaaatcattt agatgtcgca gcttttaaag aagatggacg ttttattcca
    20641 acaattgcat tttatagtcc gaatgcatta ggcgctataa ctaaagcact acattctgat
    20701 aattacagtt ttaaaaatgt atatcatgaa ttatcaacgg attatttgga tgtaagggat
    20761 gtagatgcgc cctcatattg gtacaaaaat ataaattatc agcatgattt ggacgcttta
    20821 attcaaaaat tgtaagctgt taggaggtcc acaaatggta gaacaaataa aagataaact
    20881 aggacgtccc atccgtgact tacggttatc tgtgacagat cggtgtaact ttaggtgtga
    20941 ttattgcatg cctaaagagg tatttggaga tgatttcgta tttttaccta aaaatgaact
    21001 tttaacgttt gatgaaatgg ctagaatcgc taaggtatat gcagaattag gtgtaaaaaa
    21061 aatacgcatt acaggtggag aaccattgat gcgacgggat ttagatgtac ttatagctaa
    21121 attaaatcaa atcgatggta ttgaagatat tggtttgact acaaatggtt tgttattaaa
    21181 aaagcatgga caaaagttat atgatgctgg gctgcgcaga attaatgtca gtttggatgc
    21241 tattgatgat acgctatttc aatcaatcaa taatcgtaat attaaagcga ctacgatttt
    21301 agaacaaatt gattacgcga cgtctattgg tttgaatgta aaagtaaatg ttgttataca
    21361 aaaaggtatt aacgatgatc aaatcatacc aatgcttgaa tattttaaag ataaacatat
    21421 agagattcga tttatagaat ttatggatgt tggtaatgat aatggatggg atttcagtaa
    21481 agttgtaact aaagatgaaa tgcttacaat gatagagcag cactttgaaa tcgatcctgt
    21541 agaaccaaaa tattttgggg aagtagcaaa atattatcgc cataaggata atggtgttca
    21601 atttggtttg attacaagtg tttcacaatc attttgttct acatgtacac gcgcaaggct
    21661 gtcatcagat gggaagtttt acggatgttt atttgcaact gtcgatggat ttaacgttaa
    21721 agcgtttatt cgttctggcg tgaccgacga agaattaaaa gaacaattta aagctttatg
    21781 gcaaataaga gatgatcgat attcagatga gagaactgct caaacagttg ccaatcgtca
    21841 acgtaaaaag ataaacatga attatattgg tggttaatgt gtagggacca ctacatatta
    21901 aatcattaga gatgttttaa tatttctgtc ttactcccta aaatacaata ttatttatta
    21961 aagtaaaaac ggtcatatct atgccagatt taatagaaat gatcgttttt aaagttttta
    22021 caagttggcg gggccccaac acagaagctg acagaaagtc agcttacaat aatgtgcaag
    22081 ttggcggggc cccaacatag agaatttcaa aaagaaattc tacagacaat gcaagttggg
    22141 gaacggggcc ccaacacaga aggtgacgaa aagtcagcat acaataatgt gcaagttggc
    22201 ggggccccaa catagagaat ttcaaaagaa attctacaga caatgcaagt tggggatcaa
    22261 cgaaataaat tttatgagaa tatcatttct atcccactct taagaatcac tacataataa
    22321 atctttagtg gttctttaac attgatgtca cactccatgc cattgagttg taatatatct
    22381 tttttaggta taaatgttgt cgaataaaca acaagttgtc caaaagatat aaatctaaac
    22441 aagatatagc cagcaattta atatttgtaa tagataaaat gctaagtttg atatataata
    22501 aatttaagta attgtataat aatatgaatt acaaacatct aagaagaaac ataggaggca
    22561 tcatattatg agtaataaag ttcaacgttt tatagaagca gaaagggagt taagtcagtt
    22621 aaagcactgg ttaaaaacaa cacataagat ttcaattgaa gaatttgtag tcctttttaa
    22681 agtgtatgaa gctgaaaaga ttagcggtaa agaattgagg gatacattac attttgaaat
    22741 gctatgggat acaagtaaaa tcgatgtgat tatccgtaaa atctataaaa aagagcttat
    22801 ttctaaattg cgttctgaaa cggatgaaag acaagtattc tatttctata gtacttctca
    22861 aaagaaattg ttagataaaa ttactaaaga aatagaagtg ttaagcgtta caaactaaaa
    22921 acttaaaaag catgccaatc agtacatcat aattgcgtct tggggacaga caaatgatga
    22981 atagagattg gcatgctttt tatttttgaa tataaatatt tagttcatgg catttctagt
    23041 tacatgacgt ccatgaatta agaagtaaac aagcatagta atgattgcta aagcggccat
    23101 aaagccgaag atttcactat atgaaaacat atgagtaaat aacccaagga atgatggacc
    23161 gaagccgaca cctgcatcta gaccaacgta aaaagtagat gtcgcgatac catatttaat
    23221 cgggggtgag acttttatcg caatagattg cattgcagat gataaatttc cataccctaa
    23281 acctaggcaa gcaccagcaa gtaatattaa ccagctttga tagcttgaaa ttaagcatac
    23341 aaatgaaagg aaaagcatga taaatgctgg gtagacaata atattttcat ttttatcatc
    23401 catcaatcta ccagcaatag gtctagtaat taacgatgct atagcatagc aaataaagaa
    23461 atagcttgct gcagtgacta ggtgtcgctc taaagcaaat gcttgtaaat aagttaggat
    23521 ggacgcatag gtaacgccaa ttaaaagcat aattacagca acaggaatgg cctcttttgc
    23581 aataaattga tgaatactaa atcttggttt atcaatgaca ttagtttcag ttttgttatt
    23641 tgttacttcg aaatcaactt ttataaataa tgagataatg agtccgagta tgcctaatat
    23701 gacacaaata ataaacagta agtcaattgc gtattttgta ataagtaaca tgcctagaaa
    23761 tgggccaatc gctgtaccta atactaaact taaggaaaat aaactgatgc cttcactttt
    23821 tctattaaca ggggtaacgt atgccgcaat agtacctgtt gcagttgtca caactgcagt
    23881 tgcgataccg tttatgagac gtacaaagat taaaaaagct aaagatccat caataaaata
    23941 aagtaattgc gtgataatta aagcaattaa accaataaat aataatcgtt taggtccgat
    24001 ttgatttaca aatttacctg tagcaaatcg accaattaaa gacccgacaa taaatagtcc
    24061 tacaactaat ccggctaatg aatcggaagc gtcgaaagct tgcttactat agccagcaat
    24121 aactacaagt aacaaataca tgcataggta tacgataaag ttgactaaaa agttaattgt
    24181 aaaacttttg gtgaaaatag gtgatttatt taatgagcct tgattagtca ttgtgtgcct
    24241 cccttaataa tatttcatga atttgattca tcacatttat ggtttcttgc atttgatctt
    24301 gcgaaatgtt tgttttggca actactgctt gttggagctt ttgtacatca agacaaacaa
    24361 tctcatataa ttcatgccct ttaggtgtta aagttaaaaa cttttgtcgt ttatcctcta
    24421 aactgttttc tactgttata aggtcatttt ctattagagc ttttaaagtt tttcttgcag
    24481 taggcttttc aattgcacgt ctatgtgaaa tttcaatgag agtagtgggc tgatgtttag
    24541 cgatatcgcg taagattaac cattggccat aatatatatt gtgtttttct aaaatcggct
    24601 cggctaattt taaatagggg cgatatattg ttataaaact attgaaaaat tcttgtgata
    24661 gcatagtgac atctcctaag acaaaatagt tagcttagct aaccttttta caacaatagt
    24721 aattataaaa cgggagcaat tagaaatcaa tatataatta ttaagagcaa aaataattat
    24781 actttgttaa aataagcgta attacatgta aataggggga tactaatgat attgaaattt
    24841 gatcacatca ttcattatat agatcagtta gatcggttta gttttccagg agatgttata
    24901 aaattacatt caggtgggta tcatcataaa tatggaacat tcaataaatt aggttatatc
    24961 aatgaaaatt atattgagct actagatgta gaaaataatg aaaagttgaa aaagatggca
    25021 aaaacgatag aaggcggagt cgcttttgct actcaaattg ttcaagagaa gtatgagcaa
    25081 ggctttaaaa atatttgttt gcgtacaaat gatatagagg cagttaaaaa taaactacaa
    25141 agtgagcagg ttgaagtagt agggccgatt caaatggaaa gagatacaca taaagatggt
    25201 aaggtaaagt ggcaattgct ttatataatg aatcaggatg atgatgaaat taagccacca
    25261 ttttttattc aatgggaaga aagtgattcc atgcgtacta aaaaattgca aaaatatttt
    25321 caaaaacaat tttcaattga aactgttatt gtgaaaagta aaaaccgatc acaaacagta
    25381 tcgaattggt tgaaatggtt tgatatggac attgtagaag agaatgacca ttacacagat
    25441 ttgattttaa aaaatgatga tatttatttt agaattgaag atggtaaagt ttcaaaatat
    25501 cattcggtta tcataaaaga cgcacaagca acttcaccat attcaatttt tatcagaggt
    25561 gctatttatc gctttgaacc attagtataa atatacgtaa gtgctatgag cgagaatgcc
    25621 catatgaata atgacaagca caatggaaag aatcgttaat atattattta atcgtgatga
    25681 cttaattaaa atgaaaaaga ttgataatat aaatgtgaaa aagataagta taacccgtaa
    25741 actaaagtaa ttcacggtga gaggttgact caatgtcata atgattgcaa cgatgttcat
    25801 aattataaat agacttaaaa taattgttct catatcaaac acctcattgt tagattattg
    25861 acattataac aggggtaatt gtatatgaac attaatgtgg ttgcttgagg aaaaatttat
    25921 tcattgaagt caagttggtt cattttagaa atgaatatcg tgttagatga tgaaagtata
    25981 ttgaagtata ggtaactagt tgaaaagtat taattgtacg ataacattaa atttaacacg
    26041 aaacatagat ataaaatgat tcacaattaa aatgggtaaa tttgaacttg ctaaactatt
    26101 aattggagca tggacatttc aaaaataaga gttcaaatct tacacaagct ctgaatcgac
    26161 actataagat acaaactgta taattaaagg tattgttaaa tagaaggaga tatcataaat
    26221 catggaaaag atgcatatca ctaatcagga acatgacgca tttgttaaat cccacccaaa
    26281 tggagattta ttacaattaa cgaaatgggc agaaacaaag aaattaactg gatggtacgc
    26341 gcgaagaatc gctgtaggtc gtgacggtga agttcagggt gttgcgcagt tactttttaa
    26401 aaaagtacct aaattacctt atacgctatg ttatatttcg cgtggttttg ttgttgatta
    26461 tagtaataaa gaagcgttaa atgcattgtt agacagtgca aaagaaattg ctaaagctga
    26521 gaaagcgtat gcaattaaaa tcgatcctga tgttgaagtt gataaaggta cagatgcttt
    26581 gcaaaatttg aaagcgcttg gttttaaaca taaaggattt aaagaaggtt tatcaaaaga
    26641 ctacatccaa ccacgtatga ctatgattac accaattgat aaaaatgatg atgagttatt
    26701 aaatagtttt gaacgccgaa atcgttcaaa agtgcgcttg gctttaaagc gaggtacgac
    26761 agtagaacga tctgatagag aaggtttaaa aacatttgct gagttaatga aaatcactgg
    26821 ggaacgcgat ggcttcttaa cgcgtgatat tagttacttt gaaaatattt atgatgcgtt
    26881 gcatgaagat ggagatgctg aactattttt agtaaagttg gatccaaaag aaaatatagc
    26941 gaaagtaaat caagaattga atgaacttca tgccgaaatt gctaaatggc agcagaagat
    27001 gaaaacatct gaaaagcaag ctaaaaaagc gcaaaatatg attaatgatg cgcaaaataa
    27061 aattgctaaa aatgaagatt taaaacgaga cctagaagct ttagaaaagg aacatcctga
    27121 aggtatttat ctttctggtg cactattaat gtttgctggc tcaaaatcat attacttata
    27181 tggtgcgtct tctaatgaat ttagagattt tttaccaaat catcatatgc agtatacgat
    27241 gatgaagtat gcacgtgaac atggtgcaac aacttacgat ttcggtggta cagataatga
    27301 tccagataaa gactcagaac attatggatt atgggcattt aaaaaagtgt ggggaacata
    27361 cttaagtgaa aagattggtg aatttgatta tgtattgaat cagccattgt accaattaat
    27421 tgagcaagtt aaaccgcgtt taacaaaagc taaaattaaa atatctcgta aattaaaacg
    27481 aaaatagatt aacgactgaa atctgaacgc tcataagact gtcatttgcg ttcagatttt
    27541 tttacacaat atagaatggt tgagtaaaat atttttgaat atagtgaaag agggggaagt
    27601 actgtgataa aaaagctatt acaattttct ttagggaata agtttgctat ctttttaatg
    27661 gttgttttag ttgtcttggg cggtgtatat gcgagtgcta aattgaaatt agaattacta
    27721 ccaaatgtac aaaatccagt tatttcagtt acaacaacaa tgccgggtgc aacgccacaa
    27781 agtacccaag atgaaataag tagtaaaatt gacaatcaag taagatcatt ggcatatgtg
    27841 aaaaatgtta aaacgcaatc catacaaaat gcttcaattg taacagttga atatgaaaat
    27901 aatacagata tggataaagc agaagaacag cttaaaaaag aaatcgataa aattaaattt
    27961 aaagatgaag ttggtcaacc agaattaaga cgtaattcga tggatgcttt tccggtttta
    28021 gcatattcat tttcaaataa agagaatgac ttgaaaaaag taacgaaagt actgaatgaa
    28081 caattaatac caaaattgca aacggtagat ggtgtgcaaa atgcgcaatt aaatgggcag
    28141 acgaaccgtg aaatcaccct taaatttaag caaaatgaac ttgaaaaata tgggttgact
    28201 gctgatgatg tagaaaacta tctaaaaacg gcaacaagaa caacgccact tggattgttc
    28261 caatttggtg ataaagataa atcaattgtt gttgatggtc aatatcaatc tgttgatgct
    28321 tttaaaaaca taaatattcc attaacgttg gcaggaggac aagggcaatc tcaatcccaa
    28381 agtgacaata aacaaaattc agccatgtca gacgttaatc aggcatcacc acagcaaaat
    28441 tcaaaagcgt cagcaccaaa taatataagt ggtatgccga cagcgaaact aggagattta
    28501 gctgatatta cagttggtga tgtgcgtact tctatttcta aaacgaatgg aaaggatgcg
    28561 gttaatctac aaataactaa agctcaagat gccaatacag ttcaagtagc caaagatgta
    28621 caacgtaaaa ttgatacatt cgttgatgaa aataaagatt ttaatgtcac aaaaacaatg
    28681 gatactgcaa agcctgttga gaaatcactt tatacgatgg ttgaaaaagc atcattaggt
    28741 acaatcgtgg caattatagt tattttgctg tttttaagaa acattcgtac gacggcaatt
    28801 tctattatat cgattccgtt atcacttctt atggcgctta ttgctctgaa attgagtgat
    28861 gtttcattga atatactaac gttaggtgca ttaacagtag cgattggacg tgtgatagac
    28921 gattcgattg tagttgttga aaatatttat cgacgcttaa cagattcaga agaacaacta
    28981 aaaggtgaaa atttaattat cagtgcgaca actgaagtat ttaaaccaat aatgtcatcg
    29041 acactagtta ctattatcgt cttcttacca cttgtgtttg tatcaggttc agtaggcgaa
    29101 atgtttagac cttttgcatt ggctattgca tttagtttat tagcatcgtt attagtgtca
    29161 attacactcg ttccagcgtt ggcagctaca ctatttaaaa aaggcgttaa acgtcgtaat
    29221 aaacaacatc aagaaggatt aggtgttgtt agtacaactt ataaaaaagt attacattgg
    29281 tcattaaatc ataagtggat tgtaattata ttaagtacat taattttggt tgcaactatt
    29341 gtatttggag gaccgagact aggcactagc tttatttcag caggtgacga taaattttta
    29401 gctattactt atacaccgaa gcctggtgaa acggagcaag cagtgttgaa tcatgcgaaa
    29461 gatgttgaaa aatatttaaa acagaaaaag catgtaaaaa caattcaata ctcagttggc
    29521 ggtagtagtc cagtagatcc aacgggtagt acaaatagta tggcaatcat ggttgaatat
    29581 gataatgaca cgcctaattt tgatgtagaa gcggataagg ttattaaaca tgcagatggc
    29641 tttaaacatc ctggagagtg gaaaaatcaa gatttaggaa caggtgcagg taataaatct
    29701 gtagaggtta ctgtaaaagg tccatcaatg gatgccataa aatcaactgt aaaagatatt
    29761 gaacagaaaa tgaaacaggt taaaggacta gccaatgtca aatctgattt atcgcaaaca
    29821 tatgatcagt atgaaattaa agtcgatcaa aataaagcgg cagaaaatgg tatttctgca
    29881 agtcaacttg caatgcactt gaatgaaaac ttaccagaaa aaacagttac gactgttaaa
    29941 gaaaatggta aaactgttga tgttaaagtc aaacaaaata agcaaacaga ctggtcagaa
    30001 gataagttga ataatataac tttgaaaaag ccgactggtg gtacgattaa attgggagat
    30061 atcgctacgt tagttaaaac aacgacacca agtaaattga cgcaagaaca aggagattat
    30121 gcaacgacgg tatctgctaa agtaacaaat aaagatgtgg gtggcacaac acgacaagtg
    30181 atgtctaaaa taaataattt ggacaaaccg aataatgtaa aggttaatat cggtggtgca
    30241 tcagatgata ttaacaatgc aatgacacaa ttagcctttg caatgttagc tgcaatcatt
    30301 atcgtatatt taatcctagt tattacattt aaaggtggcc tagcaccatt tacaatttta
    30361 ttctctttac catttacagt tatcggtgta attattgcac tattaatcac aggagaaaca
    30421 atatcagtac caagtttaat tggtatgcta atgttaattg gaatcgtagt aacaaatgcc
    30481 attgtgttaa tagaccgtgt tattaataat gagcaacagg gcatggagat gaaagaagca
    30541 ttaatcgaag caggcggtac tagaattaga ccgatattaa tgacggcgat tgcaacaatt
    30601 ggtgcattag ttcctttgtt atttggtcaa gatagctcga ttcttatttc gaaaggatta
    30661 gctgctacag ttattggtgg tttaatttca tcaactttat taacacttgt agttgtacca
    30721 gtaatatatg aaattctctt tactttaaaa aaacgattca ctaaacgata gataatataa
    30781 aagccataaa agcggtcatg atattgacta cttttatggc tttttaaaat ttgaaatttt
    30841 agaccttatt tcacgtgcga tttaatcgat gtgttaataa aacaatactg gcattcaaca
    30901 tgtaatccga taataaaata aggttgcaat tatgttattg catattttgt ttattactta
    30961 gaataatacg atggttaaga tgaaatatat gacaaagata aataatgaca ttaaattcaa
    31021 gatgtttacg ccttttagat cttttttaag cattttaaat cctgtcactt cgacattacc
    31081 gactaagagt aataagacga taaataccca ccaattctct tttggaaaac tcatcgtcgt
    31141 taatgctaag acgaataata acaacacacc cataataatt ctcaaaatgc gagtaaatac
    31201 aacttccatt ttaaaaatac taataaaact aacaattaga taaagtattg ctagtaaaat
    31261 cagataaaat tgcatatgat aacttcctct ccgtttcaaa gggttcatgt agtacagcag
    31321 cgtagaaaac ataaatagca gtgttgggat agatatgcgt gtatgttgtt aatgaaacac
    31381 taaatcataa cacgacaaca tcttgtgatt ctagatgtaa ttgagcgaaa aatgaagcaa
    31441 ttcatcaata taacgtattc agagcaatgc tagtcaaatt tatgtaacaa aaagcattta
    31501 ttgcttgaat gatagattat taatcttgca tttgagcacg aacgtttcta atttcatttt
    31561 caatatcttt aagttgacga acaagtgcgg aagtatctaa attcaaagat tcccgttttt
    31621 ctatgtcacc ttgaagacga acataatcat atttaagttc ggctagttgg ttttctaaag
    31681 acatactata acactccttt gaaatataat taagtgcatt taatgtagat tatagcatga
    31741 aacagtagac ttctaaaata tctgtggagt atgttaaagt tataaattat gaagattatt
    31801 gaaagaaggg ataacagtga cagaacaatt tactatgatt attttattaa ttgcgcttgg
    31861 ttactttttg aagcgcatta attttataaa agcaacagat agtcaagtca ttgctacatt
    31921 agttcttaat gtcacactcc catctttagt tattgtgaat ttaaacagtg cagaattaaa
    31981 attgtcattc tccattttac caattttaat gattatatat ggtattgtgg cgaaaatgat
    32041 tgctgtctgg ttttttagaa aatatgataa tcacatgcgt ggatcagtag gtatgatgac
    32101 tggtgctatg aatattggat tgtttgcata tccactaatt gaagtgatat ggcctaaaac
    32161 gggacttatt tattttgtta tggctgatat aggtggtgcc tttgtgatgt ttggaattac
    32221 ttactttgta ggtagctact ttagtgaagg tagtgatcaa ttcgatttta aatatttagg
    32281 taagaaatta ttacaatcag tgccactcgt tacatacatt gtaatgttta tattgaatat
    32341 aagtcacatc catttaccac atgtggcaat cgatttcttt agcatacttt cccatgcaaa
    32401 tatgccgtta tcaatgattt tgctaggtgt catgttgaat tttactctgg aaagaaagta
    32461 tatacctgct actattaaat atttatgttt gcattatgga ttagcattag ttgcgggatt
    32521 gttagtatat gtgttgttac cagtatcaga tgatatgatt aagacgacat tgttggtcac
    32581 ttggatgttc ccggtaggtg ttgcaattat ttcttatggt attcagttta aatatcgaac
    32641 actgacattt ataggtatga cgaccaattt gacaatcatt attagtatca ttattttgta
    32701 cgtgtatcag gcagtgtttg tctagtttac acatttgaaa ataaaatcca ttttagtatt
    32761 cgtagaaaat gagagccccc tttaccacaa tatgtaaatg tatattgtga aaagggggct
    32821 tttatttatg acatagttgt tcaactcata cattcaagtt gataatgcag tgcgttatgc
    32881 agctatccac ttcatatgta tggcgcatac ttgatatatg aatatcatca agattttaaa
    32941 agtatcgcta attttaaagt cttaaaacgc atgattaatg tcatgatgtg ttattgcgtt
    33001 ttgtttttag agccacgtat ttcctaggac atatagagga acattcaacg taaaattccc
    33061 gaaaattaat tttaatcatt ctataagtga atggttattt tcagaagtga ttacattttt
    33121 acgttacatg agtatttaac tttctatttc aaattaccta gaattatagc agctatcacg
    33181 ataataataa tacctgccca aatacccgtc atctgacgac gatcttttct ttctcctaaa
    33241 atgaaaatac cgcctaaggt tgaaacgata acaagtaact gtgataatga gaaacttgta
    33301 gctacaccaa cttttggttg agaatagaac atgaacaagt taccaattcc ccaaatcaca
    33361 cctggcaata gattaagtgc tgttgattta agtgatgttt tatgattcat ggataggata
    33421 aagccaccaa ttgccatacc gacagattgg aagaacaatg catcagttcc accaacacca
    33481 aagatgtcac caagtacaac gaaacctaca tatccaactg tagatacaat taaaataccc
    33541 attgcttttt taaattcagg attatctgat tgacgttcat ttttagcttt aagtgaagta
    33601 agtgctacac cagtaactaa taatatcatg gcgattaaac caaagataat ttgagtcatt
    33661 gaagaccatt cacctaaaaa gataacgctg aataatgttg taccaactaa ttgcatacct
    33721 gtagaaactg gcatagtatt tgatacaccg attaaactaa tagatttaag ctgattcgct
    33781 tgtccaaacg cccataatgc accagaaata agaccgacaa taattacagt aggattattg
    33841 aattttgcga aaccagtaat aagtaaacct aatccaacaa tgagtgcgcc tagcgtcgta
    33901 ccacgaattt ggttgtaagg tccaccgccg acgaacacat taataagaac gacacttccc
    33961 cagaataaag caggtaaaag tgcgattaag aaatcaagaa attgcaagac tactcactct
    34021 cctatactat ttatgcaagt gctcccctta attgacactt gcatgttatt gtaaagcatt
    34081 ctgccctaat agtaaaaaac aactacttat aattttcaat atttacaaaa agtagaaaaa
    34141 cccacaatac atgctaatga tgtactgtag ggtatttgct tggtgtttta ttcagggatt
    34201 gtttgatctt ttacgttacg tgattcgaat ttgaatttaa tgtactcata gttgatatgg
    34261 aaacctagac cattaaatcg ttcgatgagt ttattatttt tattacgaat tttaaaataa
    34321 acttttctaa tatcaaaatt atcaaaagca tattgcaatg catacgcaag caactcgaaa
    34381 gcgatacctt tcaaacggta atctacatga gaactgaagt atttaatctc ggcgattgac
    34441 tgttgtgaat caatttcaag gtataaataa cctttaagta aaccttcgct aacaaataaa
    34501 aacaaatgat gatggtcgtc taaactatca atgatatctt gtggtgactg tgcctgatat
    34561 ttaaatgtag ttgtgtgtaa tttgctgaaa gcacgataaa accctttgtg ataaggaatg
    34621 atatttggtt ggtgcatatc ttcttctaat cttgtacggg cttctatgta atagtcagtg
    34681 aagttataac ttgcttgaat aactttcatt aatggtttgt attgttgaat gccttcttca
    34741 aaagaaaagt taaagacggc atcgtcaggt tggttcgaag tcattgcttt aaatagcgtt
    34801 tcaaacgtat cgacagataa tacataattg tcagccacga aagggcctat gactttgaat
    34861 ttatttttat cgtatgcaaa agacaatatg attgctttga ttgaatcgtt ttctttaatc
    34921 gaaaatacac caggtgattc aatcgcatcg gttattaata catctatttc attgtactgt
    34981 tgaggaagtt tatataagta tgatgctgat tcataattcg aatgttcaat gaacgatttt
    35041 atttgtgtaa tatcatataa ctgaactatg ctcatgtcat tacctccgta ctttttgtta
    35101 cttttattat atagtatttc aactgaaatg aaagttaata gtgatattaa catgttacaa
    35161 tacatttaac acatcatttg caatcattga atttaaatca aagattagtg gaatagatga
    35221 agcacgttcg aaataaaaga acgtatgaga aaggataatt tatgaaatct ttaatattag
    35281 ctgaaaaacc atcagtcgca agagatattg ctgatgcttt acaaataaat cagaagcgta
    35341 atggttactt tgaaaataac caatatattt tcacgtgggc gttaggtcat ctagtgacaa
    35401 atgcgacacc tgaacaatac gataaaaatt taaaggaatg gcgattagaa gaccttccaa
    35461 ttatacctaa atatatgaaa actgttgtta ttggtaaaac aagcaaacaa tttaaaacag
    35521 taaaagcgtt aattttagat aataaagtga aagatattat tattgcaaca gatgctggac
    35581 gagaaggtga actagttgca agattgattt tggataaagt tggtaacaaa aagccaatcc
    35641 gtcgattatg gattagctca gttactaaaa aagctattca acaaggtttt aaaaatttaa
    35701 aagacggtcg tcaatataac gatttgtatt atgcagcgtt agcgagaagt gaggcagatt
    35761 ggattgttgg gattaatgca acgcgtgcac taacaacaaa gtatgatgca cagctatccc
    35821 tgggacgtgt tcagacacca acgattcaat tagtaaatac acgacaacaa gagattaatc
    35881 agttcaaacc acaacaatac tttacattat cattaacggt aaaagggttt gattttcagc
    35941 tagaatcaaa tcagcgatat accaataaag aaactttaga acagatggtt aataatttga
    36001 aaaatgtcga tggtaagatt aaatctgttg ctactaaaca taagaagtcg tatccgcaat
    36061 cactgtacaa tttaacagat ttacaacaag atatgtatag acgttataaa attggaccta
    36121 aagaaacatt gaatacactt caaagcttat atgagagaca taaagtcgta acctatccaa
    36181 gaacagattc aaactattta acaactgata tggtagatac tatgaaagaa cgtattcagg
    36241 cgacgatggc aacaacatat aaagaccaag cacgcccatt aatgtctaaa acattttcat
    36301 caaaaatgtc gatatttaat aatcaaaaag tatctgatca ccatgcaatt attcctacag
    36361 aagtgagacc tgtcatgtca gacttaagta atagagaatt aaagttatac gatatgattg
    36421 tcgagcgttt tttagaagct ttaatgcctc cgcacgagta tgacgcgata actgtaactt
    36481 tagaggttgc agggcacaca tttgttttga aagagaatgt aacaactgtt ttaggtttta
    36541 aatctattag acaaggtgaa tctattacag agatgcaaca gcctttttca gaaggcgatg
    36601 aagtgaagat ttcaaaaaca aatattagag aacatgaaac aacacctcca gaatatttta
    36661 atgaaggttc gttattaaaa gcgatggaga accctcagaa ctttattcaa ttgaaggata
    36721 aaaaatatgc gcaaacttta aaacaaacag gtggtatcgg cacagttgca acaagggccg
    36781 acattatcga taaattattt aatatgaatg ccattgaatc aagagacggt aaaattaaag
    36841 taacgtcaaa aggtaaacaa atattagaat tagcaccaga agaattaacg tcgccacttt
    36901 taactgcaca atgggaagaa aaattacttt taattgaacg tggtaaatat caggcgaaaa
    36961 catttattaa tgaaatgaaa gattttacga aagatgttgt aaatgggatt aaaaatagtg
    37021 atcgtaaata taaacacgat aatttaacaa ccacagaatg cccaacgtgt ggtaaattca
    37081 tgattaaagt taaaactaaa aatggtcaga tgcttgtgtg ccaagatcca tcttgtaaga
    37141 cgaaaaagaa tgtacagcgc aaaacaaatg caagatgtcc aaactgtaaa aagaaattaa
    37201 cgttgtttgg taaagggaaa gaagcggtat atcgttgtgt ttgtggacat tctgaaacgc
    37261 aagcacatat ggatcagcgt atgaagtcta aatcctctgg taaagtatct cgtaaagaaa
    37321 tgaaaaagta tatgaataaa aatgaaggtt tagacaataa tccgtttaaa gatgcattaa
    37381 agaacttgaa tttatagata aaatcgaaca aagttgaatc agaaaaacga aaagttcgct
    37441 tttggtattg ttttttatta agaatgatat taaactatta aggtatttta aaaaaaggag
    37501 catccattcg tgaaaaacta tttccagttc gataaatatg gaacaaactt taaaagagaa
    37561 atcttaggcg gtatcacaac tttcttatct atggcctata ttttagcagt taacccgcaa
    37621 gttttaagtt tagcaggtgt taaaggcgta tcagaagata tgaaaatgga ccaaggtgcc
    37681 atttttgtag cgactgcatt agcagcattt gtaggctcgc tattcatggg gctaatagct
    37741 aaatatccaa ttgcgttagc accaggtatg ggattaaatg cattctttgc atttactgtt
    37801 gtgttaacca tgggtattcc ttggcaagtt ggtttaacag gcgttttatt ctcaggtata
    37861 ttctttgcga tattaaccgt cacagggttt agggaagtta ttattaatgc aattccttat
    37921 caaatgaaga tggctgtttc agcaggtata ggattattca ttacatttgt aggtttacaa
    37981 agttcgggta ttatagttaa aaatgaatct acattagtaa cattgggaca tttaactgat
    38041 ccaccagtac ttttggcgat attcggtatc gttattacag ttattttata tgcaattaaa
    38101 ttgccgggtt ctatctttat cggtatgatc atcacagcaa tcgttggtat gttcactgga
    38161 ttgattcaaa tgccatctgg tattgtgggt aagattccaa gtattgaacc aacgttcggt
    38221 gctgcatttg aagcgtttaa agacccaagt caattattaa cgatacagtt cttaattgtt
    38281 attttaacgt tcttatttat tgatttcttt gatacagctg gtacattagt agctgttgct
    38341 actcaagcag gtattatgaa agataataaa ttaccaagag ctggtagagc attgttctca
    38401 gattctttag ctacgattgt aggatcagtt tttggaacaa ctacaacaac ttcttatatc
    38461 gaatcaactt caggtgttgc tgtaggtgct agaacaggat ttgctagtat cgtaacaggt
    38521 tgttgtttcc tattagcgct gttctttagt ccgttaatcg ctgttgtaac aagtgcagtt
    38581 acgacaccag ccttagtggt ggtaggtgta ttaatggctg caaactttgc tgagataaac
    38641 tggaaaagtt ttgaagttgc agtgccagcc ttcattacaa tcattatgat gcctttatca
    38701 tattcaattg caactggtat tgcgtgtgga tttattttct atccaattac aatgcttatc
    38761 tctaaaaaac ataaagaagt acatccgata atgtatgtat taatggtact attcattctg
    38821 tacttcatat ttgttcatgg ttaaatccag acacaaaaaa cgactgccaa tgattggtag
    38881 tcgttttttt ctttgaagta aggataaaat aagaagcgaa ataacataaa gcgatattat
    38941 gaatgtcttt aaatatccga tataaatata cttcttttaa aattgttgga cgtgtttctt
    39001 ctatattata tacctaatta aaaaggtttt aagaattatt ctctatgtcc atctttgtat
    39061 tgtgtgtaaa tgacacgagc atttttatta ggtatatagg ttggcgctga taatacaact
    39121 aggacaaata gaatcattag gaaaaataaa atccaatgaa tcaaaattcc taagaagggg
    39181 ataatagcta aaaaactacc tactatacca aatattggaa taatcgctat aggtctaatc
    39241 gcattttgtc tattcactaa taatataata gtaagaatta gataaagtaa tgcattaaag
    39301 agtaaaggct gccatccaaa acttaaaata aacgttgcac ctaaaaaagg aatgccatag
    39361 aaaaactcac aaagtattaa aaagatacct ataagtgcta aagataattt tggtccattt
    39421 ttcatattca ttcaccaccg ttcttatgac taattatata gaataatttt caatgataat
    39481 aataaaatga ttcaaggtac gaatttacaa taaagaagga aataaaaaat atctcgaaaa
    39541 tagttgaact gactaagtat ataaagtaaa atataaaagt atgtgttaga cagatagaaa
    39601 aattatgtga aaagccttga tttatcgatg tttttcgtgt aatataacta acgttggact
    39661 ttaagaaaag cgatgaagcg aaaggttact gacacacccg gccgctttgc catggcgctg
    39721 tgtaagatag ttttcgtgga gaagtctatc actaaatgta gacgaataag gagggaaaat
    39781 tatggcaaaa caaaaaatca gaatcagatt aaaagcttat gatcaccgcg taattgatca
    39841 atcagcagag aagattgtag aaacagcgaa acgttctggt gcagatgttt ctggaccaat
    39901 tccgttacca actgagaaat cagtttacac aatcatccgt gccgtgcata agtataaaga
    39961 ttcacgtgaa caattcgaac aacgtacaca caaacgttta atcgatattg taaacccaac
    40021 accaaaaaca gttgacgctt taatgggctt aaacttacca tctggtgtag acatcgaaat
    40081 caaattataa tagacaattt taggaggtgg actttcgatg accaaaggaa tcttaggaag
    40141 aaaaattggg atgacacaag tattcggaga aaacggtgaa ttaatccctg taacagtagt
    40201 agaagctaaa gaaaatgttg tattacaaaa gaaaactgta gaagttgatg gatacaacgc
    40261 aatccaagtt ggatttgaag acaaaaaagc atacaaaaaa gatgcaaaat ctaataaata
    40321 tgctaataaa ccagctgaag gtcacgctaa aaaagctgac gcagcaccta agcgcttcat
    40381 tcgtgaattc cgcaatgtag acgtggatgc ttacgaagta ggtcaagaag tctcagtaga
    40441 tacttttgta gctggcgacg ttattgacgt aacaggcgta tcaaaaggta aaggtttcca
    40501 aggtgcaatt aaacgccacg gacaatctcg tggacctatg tcacacggtt ctcatttcca
    40561 cagagcacca ggttctgtag gtatggcttc agatgcttct agagtattta aaggccaaaa
    40621 aatgccagga cgtatgggtg gaaacactgt aactgttcaa aacttagaag tagttcaagt
    40681 tgacacagaa aacaaagtta tcttagtaaa aggtaacgta cctggaccta aaaaaggttt
    40741 agtagaaatc agaacttcaa ttaaaaaagg taataaataa taagaagtga aaggaggaaa
    40801 tgcataatgg ctaattatga tgttttaaaa ttagacggaa ctaaatcagg ttcaatcgaa
    40861 ttaagcgatg cagtattcgg tattgagcca aataatagcg ttttattcga agctattaat
    40921 ttacaacgtg cttcattacg tcaaggtacg catgctgtta agaatcgttc agcagtaagc
    40981 ggtggcggac gtaaaccatg gaagcaaaaa ggaacaggtc gtgctcgtca aggtacaatc
    41041 cgtgctccac aatggcgtgg cggtggtatc gtattcggac caactccaag aagttatgca
    41101 tacaaaatgc ctaagaaaat gcgtcgttta gctttacgct cagcattatc tttcaaagct
    41161 caagagaatg gcttaactgt agttgacgca ttcaacttcg aagctccaaa aactaaagaa
    41221 ttcaaaaatg tattatctac attagaacaa cctaaaaaag tattagtagt tactgaaaac
    41281 gaagatgtaa atgttgaatt atcagcacgc aacatccctg gcgttcaagt gacaactgct
    41341 caaggtttaa atgttttaga tatcactaat gctgacagct tagtaattac tgaagctgct
    41401 gctaaaaaag ttgaggaggt gctcggataa tggaagcaag agatattctt aagcgccccg
    41461 taatcactga gaaatcttct gaagcaatgg ctgaagacaa atacactttc gacgttgata
    41521 ctcgtgttaa caaaacacaa gtaaaaatgg cagttgaaga aatcttcaac gtaaaagttg
    41581 caagtgttaa tatcatgaat tacaaaccta agaaaaaacg tatgggccgt taccaaggct
    41641 atacaaacaa aagaagaaaa gcgattgtaa ctcttaaaga aggatcaatc gacttattta
    41701 actaataaaa caattaccat taaggaggta agcgacaatg gctattaaaa agtataagcc
    41761 aataacaaat ggtcgtcgta atatgacttc gttagatttc gcagaaatca cgaaaactac
    41821 acctgaaaag tcattattaa aaccgctacc gaaaaaagcg ggacgtaaca accaaggtaa
    41881 attgactgta agacaccatg gtggtggaca caaacgtcaa taccgtgtta tcgatttcaa
    41941 acgtaacaaa gatggtatca atgcaaaagt tgattctatt caatatgatc caaaccgctc
    42001 agcaaacatc gctttagttg tatatgcaga cggtgaaaaa cgatatatca ttgctcctaa
    42061 aggattagaa gtaggtcaaa tcgttgaaag tggtgctgaa gctgacatca aagttggtaa
    42121 cgcattacca ttacaaaaca ttccagttgg tacagtagta cacaacatcg agcttaaacc
    42181 tggtaaaggt ggacaaatcg ctcgttcagc tggtgcaagt gctcaagtac ttggtaaaga
    42241 aggtaaatac gtattaatca gattaagatc tggtgaagtt cgtatgatct tatctacttg
    42301 ccgtgctaca atcggtcaag ttggtaacct acaacacgaa ttagttaacg ttggtaaagc
    42361 cggacgttca agatggaaag gtatccgtcc aacagttcgt ggttctgtaa tgaaccctaa
    42421 cgatcaccca cacggtggtg gtgaaggtcg tgctcctatc ggtagaccat ctccaatgtc
    42481 accatggggt aaacctacgc ttggtaagaa aactcgtcgt ggtaaaaaat catcagacaa
    42541 acttatcgtt cgtggacgta agaaaaaata atataatcaa cttatttggg tgtgcggctt
    42601 aaagctgcac gcacataata agaagggagg cgcccaaatg gctcgtagta ttaaaaaagg
    42661 acctttcgtc gatgagcatt taatgaaaaa agttgaagct caagaaggaa gcgaaaagaa
    42721 acaagtaatc aaaacatggt cacgtcgttc tacaattttc cctaatttca tcggacatac
    42781 ttttgcagta tacgacggac gtaaacacgt acctgtatat gtaactgaag atatggtagg
    42841 tcataaatta ggtgagtttg ctcctactcg tacattcaaa ggacacgttg cagacgacaa
    42901 gaaaacaaga agataatatc tattaagtag aggaggacat cctaatggaa gcaaaagcgg
    42961 ttgctagaac aataagaatc gcacctcgta aagtaagact agttcttgac ttaatcagag
    43021 gtaaaaatgc tgctgaagct attgcaattt taaaattaac aaacaaagct tcatcaccag
    43081 taattgaaaa agtattaatg tccgctttag ctaatgctga acataactat gacatgaaca
    43141 cagatgaatt agtagttaaa gaagcatatg ctaacgaagg accaacatta aaacgtttcc
    43201 gtccacgtgc gcaaggtcgt gcaagtgcga ttaacaaacg tacaagccac attacaatcg
    43261 tcgtaagtga cggtaaagaa gaagctaaag aagcttaatt aacttttaag gagggaatac
    43321 tgtgggtcaa aaaattaatc caatcggact tcgtgttggt attatccgtg attgggaagc
    43381 taaatggtat gctgaaaaag acttcgcttc acttttacac gaagatttaa aaatccgtaa
    43441 atttattgat aatgaattaa aagaagcatc agtttctcac gtagagattg aacgtgctgc
    43501 aaaccgtatc aacattgcaa ttcatactgg taaacctggt atggtaattg gtaaaggcgg
    43561 ttcagaaatc gaaaaattac gcaacaaatt aaatgcgtta actgataaaa aagtacacat
    43621 caacgtaatt gaaatcaaaa aagttgatct tgacgctcgt ttagtagctg aaaacatcgc
    43681 acgtcaatta gaaaaccgtg cttcattccg tcgtgtacaa aaacaagcaa tcactagagc
    43741 tatgaaactt ggtgctaaag gtatcaaaac tcaagtatct ggtcgtttag gcggagctga
    43801 catcgctcgt gctgaacaat attcagaagg aactgttcca cttcatacgt tacgtgctga
    43861 catcgattat gcacacgctg aagctgacac tacttacggt aaattaggcg ttaaagtatg
    43921 gatttatcgt ggagaagttc ttcctactaa gaacactagt ggaggaggaa aataataatg
    43981 ttactaccaa aacgtgtaaa atatcgtcgt caacatcgtc ctaaaacaac tggtcgttct
    44041 aaaggcggta actacgtaac atttggtgag tttggtttac aagctacaac aacgtcttgg
    44101 atcacatctc gtcaaatcga atctgctcgt atagcaatga cacgttacat gaaacgtggc
    44161 gggaaagttt ggattaaaat cttcccacat acaccatata ctaaaaaacc tttagaagta
    44221 cgtatgggtg ctggtaaagg tgcggttgaa ggctggatcg cagttgttaa accaggtaga
    44281 attttattcg aagttgctgg cgtttctgaa gaagttgcgc gtgaagcact acgtttagca
    44341 agtcacaaac ttccagtaaa aactaagttt gtaaaacgtg aggaattggg tggtgaaaca
    44401 aatgaaagct aaggaaatta gagacttaac cacttcagaa atcgaagaac aaatcaaatc
    44461 ttcaaaagaa gagcttttta acctacgctt tcagttagct acaggtcaat tagaagaaac
    44521 tgcacgtatt cgtacagtaa gaaaaacgat tgcacgtcta aaaactgttg ctcgtgaaag
    44581 agaaattgaa caaagtaagg ctaatcaata attaagtaag aggaggttac aaaagtgagc
    44641 gaaagaaacg atcgtaaagt ttatgtaggt aaagttgttt cagacaaaat ggacaagact
    44701 attacagtac ttgttgaaac ttacaaaaca cacaaattat acggtaaacg agtaaaatac
    44761 tctaaaaaat acaaaactca tgatgaaaac aattcagcta aattaggaga cattgttaaa
    44821 attcaagaaa ctcgtccttt atcagcaaca aaacgttttc gtttagtaga gattgttgaa
    44881 gagtcagtaa ttatttaata caagtttaga gataagggag gtttaactaa tgatccaaca
    44941 agaaacacgc ttgaaagtag cagacaactc tggtgctcgt gaagttctta caatcaaagt
    45001 attaggtgga tctggtcgta aaacagcaaa catcggcgat gttatcgtat gtactgttaa
    45061 aaatgcaaca ccaggtggcg ttgttaaaaa aggtgacgtt gtcaaagctg taatcgtacg
    45121 tactaagtca ggtgttcgtc gtaatgacgg ttcatacatc aaatttgatg aaaatgcatg
    45181 tgttatcatc cgtgatgaca aaggcccacg tggtactcgt atcttcggac ctgttgctcg
    45241 tgaattacgt gaaggtaact tcatgaaaat cgtatcatta gcaccagaag tactttaatt
    45301 aataacaacc aatacaaata aggaggtgcc cacatgcata tcaaaaaagg tgacaacgtt
    45361 aaagttatcg caggtaaaga caaaggtaaa gaaggtaaag taattgctac tctacctaaa
    45421 aaagaccgtg tcgttgtgga aggtgttaac attatgaaaa aacaccaaaa accaactcaa
    45481 ttaaatcctg aaggtggaat cttagaaaca gaggcagcaa tccatgtttc taatgtacaa
    45541 ttattggacc ctaaaacaaa cgaaccaact cgtgtaggtt acaaatttgt tgatggtaaa
    45601 aaagttcgta tcgctaaaaa atctggcgaa gaaattaaat ctaataatta ataacaagtt
    45661 gaaaggagga tcccactttg aaccgtttaa aagaaaagtt taacactgaa gttactgaaa
    45721 acttaatgaa aaaattcaat tatagttcag taatggaagt accaaaaata gataaaatcg
    45781 ttgtgaacat gggtgtaggt gacgcagtac aaaattctaa agtattagac aatgctgttg
    45841 aagaattaga attgatcact ggtcaaaaac cattagtaac taaagctaaa aaatcaatcg
    45901 cgactttccg tttacgtgaa ggtatgccaa tcggtgcgaa agtaacactt cgcggtgaaa
    45961 gaatgtatga attcttagac aaattaattt cagtatcatt accacgtgta cgtgacttcc
    46021 aaggtgtttc taaaaaagca tttgacggac gcggtaacta cactttaggt gttaaagaac
    46081 aattaatttt cccagaaatc gactatgata aagtaagtaa agttagagga atggatattg
    46141 ttatcgtaac gactgctaac actgatgaag aagctcgtga attgttagct aacttcggta
    46201 tgccattccg taaataatta tttaaaggag gctaattaag tggctaaaac ttcaatggtt
    46261 gctaagcaac aaaaaaaaca aaaatatgca gttcgtgaat acactcgttg tgaacgttgt
    46321 ggccgtccac attctgtata tcgtaaattt aaattatgcc gtatttgttt ccgtgaatta
    46381 gcttacaaag gccaaatccc tggcgttcgt aaagctagct ggtaataaaa aagagtctga
    46441 aaggaggcaa caatcaatga caatgacaga tccaatcgca gatatgctta ctcgtgtaag
    46501 aaacgcaaac atggtgcgtc acgagaagtt agaattacct gcatcaaata ttaaaaaaga
    46561 aattgctgaa atcttaaaga gtgaaggttt cattaaaaat gttgaatacg tagaagatga
    46621 taaacaaggt gtacttcgtt tattcttaaa atatggtcaa aacgatgagc gtgttatcac
    46681 aggattaaaa cgtatttcaa aaccaggttt acgtgtttat gcaaaagcta gcgaaatgcc
    46741 taaagtatta aatggtttag gtattgcatt agtatcaact tctgaaggtg taatcactga
    46801 caaagaagca agaaaacgta atgttggtgg agaaattatc gcatacgttt ggtaataaaa
    46861 aataaggagg tgccataaca tgagtcgtgt tggtaagaaa attattgaca tccctagtga
    46921 cgtaacagta acttttgatg gaaatcatgt aactgttaaa ggtcctaaag gtgaattatc
    46981 aagaacttta aatgaaagaa tgacattcaa acaagaagaa aacacaattg aagttgtaag
    47041 accatctgat tctaaagaag atagaacaaa ccatggtaca actcgtgctt tattaaacaa
    47101 tatggtacaa ggtgtttctc aaggatacgt aaaagtactt gaacttgttg gtgtaggtta
    47161 ccgtgctcaa atgcaaggta aagacttaat ccttaacgtt ggttattctc acccagtaga
    47221 aattaaagct gaagaaaaca ttactttctc agttgagaaa aacacagtcg ttaaagttga
    47281 aggtatttca aaagaacaag ttggagcatt agcatctaac atccgttcag taagacctcc
    47341 agagccttac aaaggtaaag gtattcgtta ccaaggtgaa tacgttcgcc gtaaagaagg
    47401 taaaactggt aaataataga taaactctaa agaaaggagt attgaaatga tcagtaaaat
    47461 tgataaaaat aaagtgcgtt taaaaagaca tgctcgtgtt cgtactaact tatcaggtac
    47521 agctgaaaag ccacgtttaa acgtatatcg ttcaaacaag catatctacg ctcaaattat
    47581 tgatgataat aaaggcgtaa cattagctca agcttcttca aaagacagcg acattgctac
    47641 tacagcaact aaagttgaat tagcaactaa agtcggtgaa gcaattgcta aaaaagctgc
    47701 tgacaaaggc attaaagaaa tcgtatttga ccgtggagga tatttatatc acggacgtgt
    47761 taaagcatta gctgaagcag caagagaaag cggattagaa ttttaattta aaggagggac
    47821 aaatacatgg ctcgtagaga agaagagacg aaagaatttg aagaacgcgt tgttacaatc
    47881 aaccgtgtag caaaagttgt aaaaggtggt cgtcgtttcc gtttcactgc attagttgta
    47941 gttggagaca aaaatggtcg tgtaggtttc ggtactggta aagctcaaga ggtaccagaa
    48001 gcaatcaaaa aagctgttga agcagctaaa aaagatttag tagttgttcc acgtgttgaa
    48061 ggtacaactc cacacacaat tactggccgt tacggttcag gaagcgtatt tatgaaaccg
    48121 gctgcacctg gtacaggagt tatcgctggt ggtcctgttc gtgccgtact tgaattagca
    48181 ggtatcactg atatcttaag taaatcatta ggatcaaaca caccaatcaa catggttcgt
    48241 gctacaatcg atggtttaca aaaccttaaa aatgctgaag atgttgcgaa attacgtggc
    48301 aaaacagtag aagaattata caattaagga gggaaaacta gttatggcta aattacaaat
    48361 taccctcact cgtagtgtta ttggtcgtcc tgaaacacaa cgtaaaactg ttgaagcttt
    48421 aggtcttaaa aagactaaca gttcagtagt tgttgaagat aaccctgcta ttcgtgggca
    48481 aatcaacaaa gttaagcact tagtaacagt agaagaaaaa taatcaagga ggtgccgaaa
    48541 tgaaattaca tgagttaaaa ccggcagaag gttcacgtaa agaacgcaat cgtgttggac
    48601 gtggtgttgc gacaggtaac ggtaaaacaa gtggtcgcgg acacaaaggt caaaaagctc
    48661 gttcaggcgg tggtgtaaga ccaggatttg aaggtggtca attaccatta ttccgtcgtt
    48721 taccaaaacg tggttttact aacataaatc gtaaagaata tgctattgtt aacttagacc
    48781 aacttaataa atttgaagat ggtactgaag taactccagc tttattagta gaatctggtg
    48841 ttgttaagaa tgaaaaatct ggtatcaaaa tactaggtaa tggttcactt gataagaaat
    48901 tgacagtgaa agctcataaa ttctcagctt cagcagcaga agctattgat gctaaaggtg
    48961 gagcacacga ggtgatctaa tgattcaaac ccttgtgaac ttctttagaa caaaagaggt
    49021 tcgtaacaag attttcttca cactagcaat gttagtaatt tttaaaatag ggacttatat
    49081 accagctcca ggagtaaatc ctgcagcttt tgataatccc caaggttctc aaggtgccac
    49141 tgagttatta aatacttttg gtggcggagc cttgaaacga ttttctattt ttgcaatggg
    49201 tattgtaccc tacatcactg catcaatcgt aatgcaatta ttacaaatgg atattgtccc
    49261 taaattctca gaatgggcaa aacaaggtga agtaggtaga agaaagttaa ataacgttac
    49321 tcgttattta gcaatttctt tagcatttat ccaatctata ggtatggcat tccaatttaa
    49381 taattatctc aaaggtgcgc tgattatcaa tcagtcaatt atgagttatt tattaatagc
    49441 actagttttg acagcaggaa ctgctttctt aatatggctt ggtgatcaaa tcactcagtt
    49501 cggtgttggt aatggtattt ctattatcat atttgcgggt attttatcaa cgttgccagc
    49561 cagtctaatt caatttggac aaactgcgtt cgttgggcaa gaagatacat cattagcatg
    49621 gttgaaagta ttaggacttt tagtgtcact gatactatta actgtaggtg ctatttatgt
    49681 acttgaagcc gttcgtaaaa ttccgattca atatgctaag aaacaaactg ctcaaagatt
    49741 aggttcacag gcaacttatc tacctttaaa agtgaactca gccggtgtta ttcctgtaat
    49801 ctttgcaatg gcgttcttct tactgcctag aacattaacg ttgttctatc cagataaaga
    49861 atgggcacaa aacattgcga atgctgcaaa tccttcaagt aatgttggta tggttgtata
    49921 tattgtacta attatactat tcacatactt ctatgcattt gtacaagtta atccggaaaa
    49981 aatggctgat aaccttaaga aacaaggtag ctatgttcca ggtattagac ctggtgaaca
    50041 aaccaaaaaa tatattacta aagttttata tcgtttaact tttgtaggtt ctatcttctt
    50101 agccgttatt tcaattctac cgatattagc aactaagttt atgggattac cacaatcaat
    50161 tcagattggt ggtacaagtt tacttatcgt tattggtgta gcgattgaaa caatgaaatc
    50221 tttagaagct caagtatctc aaaaagaata taaaggcttt ggtggtagat aattttagga
    50281 gggcacttat gaatatcatt ttgatgggtt tacctggcgc aggtaaagga actcaagcaa
    50341 gtgaaattgt caagaaattc ccaatacccc acatttcaac tggtgacatg ttcagaaaag
    50401 ctataaaaga agaaactgaa ttaggtaaag aagctaagtc ttatatggac cgtggcgaat
    50461 tagttcctga tgaagtgact gtaggtatcg ttaaggaaag aatttctgaa gacgatgcaa
    50521 aaaaaggctt tttattagat ggcttcccaa gaacaatcga gcaagctgag gcattaaata
    50581 atattatgtc tgagcttgac agaaacattg atgctgtcat caatatcgaa gttccggaag
    50641 aagaattaat gaaccgtctt acaggtcgtc gaatctgtga gtcatgtggt acaacgtatc
    50701 atcttgtatt taatcctccg aaggtcgaag gtatttgtga tatcgatggt ggtaaattgt
    50761 atcaacgaga agatgataat cctgaaacgg tagctaatcg tttgagtgtt aatattaaac
    50821 aatctaagcc tattttagat ttctatgatc aaaaaggtgt attgaaaaat attgatggtt
    50881 caaaagatat tagcgacgtt accaaagatg tcattgatat tttagatcat ttgtaataga
    50941 tcaactttta gcggtctatt ttaagtgagg catgatttaa tcatattaga tagatgagag
    51001 tggcaattaa cgataattaa ttcccgtaat ttttgtttaa cttataaaat taatcactga
    51061 tagattcgta acaataaagt tgtctattga cgattacctt actattgcaa aaagggggaa
    51121 gttaatcaat ggctaaacaa gatgtaattg aattagaagg tactgtatta gatactttac
    51181 cgaacgcaat gtttaaagta gaattagaaa atggtcatga gattttagct cacgtaagtg
    51241 gtaaaatcag aatgaattac attcgtattc tacctggcga caaagtaact gttgagatgt
    51301 ctccgtacga tttaacacgc ggaagaatta cttatcgtta taaataatcg tcactccata
    51361 atatagggag gtataaaaat gaaagtaaga ccatcagtaa aacctatttg cgaaaaatgt
    51421 aaagtcatta aacgtaaagg taaagtaatg gtaatttgtg aaaatccaaa acacaaacaa
    51481 agacaaggtt aataaaagag aggtgtaaat taatatggca cgtattgcag gagtagatat
    51541 tccacgtgaa aaacgcgtag ttatctcatt aacttatata tacggtatcg gtacgtcaac
    51601 tgctcaaaaa attcttgaag aagctaacgt atcagctgat actcgtgtga aagatttaac
    51661 tgatgacgaa ttaggtcgca tccgtgaagt tgtagacggt tataaagtcg aaggtgactt
    51721 acgtcgtgaa actaacttaa atatcaaacg tttaatggaa atttcatcat accgtggtat
    51781 ccgtcaccgt cgtggtttac cagttcgtgg tcaaaaaacg aaaaacaacg cgcgtactcg
    51841 taaaggacca gttaaaacgg tagctaacaa gaaaaaataa taggtaaagg aggcaaattt
    51901 taaatggcac gtaaacaagt atctcgtaaa cgtagagtga aaaagaatat tgaaaatggt
    51961 gtagcacaca tccgttcaac attcaacaac actattgtaa ctatcactga tgagttcggt
    52021 aatgctttat catggtcatc agctggtgca ttaggattca aaggatctaa aaaatcaaca
    52081 ccatttgcag cacaaatggc ttctgaaact gcatctaaat cagctatgga gcatggttta
    52141 aaaacagttg aagtaacagt taaaggacct ggtccaggtc gtgaatcagc tattcgtgca
    52201 ttacaatctg caggtttaga agtaactgcg atcagagacg ttactccagt acctcataac
    52261 ggttgtcgtc caccaaaacg tcgtcgtgta taatttatga tggtattgtt acaggtcact
    52321 gagcaaacat tttaaattaa gtcgacgtat ataaggagga tatttaaatg atagaaatcg
    52381 aaaaacctag aattgagaca attgaaatta gtgaagatgc taaattcggt aagttcgttg
    52441 ttgaaccact agaacgtggc tacggtacta cactaggaaa ctccttacgt cgtatcctac
    52501 tatcttcatt accaggtgca gccgttaagt atattgaaat tgagggagtt ttacatgaat
    52561 tctcagcagt agacaatgta gttgaagatg tttctacaat tattatgaac attaaacaat
    52621 tagcattgaa aatttactct gaagaagata aaactttaga aattgatgta cgtgatgaag
    52681 gcgaagtaac agcaagcgac attacacatg atagtgatgt tgaaatttta aacccagagc
    52741 ttaaaattgc aacagtatct aaaggtggtc acttaaaaat tcgtctagtt gctaacaagg
    52801 gtagaggtta cgcattagca gaacaaaata atactagtga tttaccaatt ggtgtaatcc
    52861 ctgttgattc attgtattca cctgttgaac gtgtgaacta tactgttgaa aatacacgtg
    52921 taggtcaaag cagtgatttt gataaattaa cattggatgt ttggactaat ggttcaatca
    52981 caccacaaga atcagtttca ttagcagcaa aaataatgac tgaacacttg aatatcttcg
    53041 ttggtcttac tgatgaagcg caaaacgctg aaatcatgat tgaaaaagaa gaagatcaaa
    53101 aagaaaaagt attagaaatg tctattgaag aattagactt atctgtacgt tcatataact
    53161 gcttaaaacg cgcaggaatc aattctgttc aagagttagc tgacaaatct gaagctgaca
    53221 tgatgaaagt gcgtaattta ggtcgtaaat ctttagaaga agttaaatac aaattagaag
    53281 atttaggatt aggattaaga aaagaagatt gataaaggag gttaactcat gggttacaga
    53341 aaattaggtc gtacttctga tcaacgtaaa gctatgttac gtgacttagc tacatcactt
    53401 attattagtg aacgtattga aactacagaa gctcgtgcaa aagaagttcg cagtgttgtt
    53461 gagaaattaa tcactttagg taaaaaagga gatttagctt ctcgtcgtaa tgcagctaaa
    53521 actttacgta atgttgaaat cttaaacgaa gatgaaacta cacaaactgc acttcaaaaa
    53581 ttatttggtg aaatcgcaga gcgttacaca gaacgtcaag gtggttacac tcgtatcctt
    53641 aaacaaggcc ctcgtcgcgg tgacggtgct gaatcagtaa ttatcgaatt agtataagaa
    53701 tttatataaa tacactaact acttttatat aaagcaaatg agtgcaacga taatgtttgc
    53761 cacatacaaa tattgtctag ctcagagtac cccatcaact aaataaagat ttaaagcgtg
    53821 aactcaacaa acttatgatg gggtgcgcgc ttttttattg aataaaatat cggacttatg
    53881 aacagaaatg tttttaagtc cgatttcttt atatttactt ttttgagtgc gaatctaaaa
    53941 atatgatgaa gctagtttga tcaatttcac tagaactgaa tgaagtgaaa gtattatcgt
    54001 tgaatcaaac gcgaccaccc ctatattagt aaatcattta ttttattaac gtttatacat
    54061 aattgttaga gaggaataaa agtccttatc attagttgtt aaacaacact ttaaagacta
    54121 gaaatgagtc agaaaatact agtaatatat agagaaccca gtcaatgtca tatacagcga
    54181 tttataaata ttgtaaaata tgatagatac acgaatcgta aaggagagag acaagtggag
    54241 gataagaatt cagttattgt atttaaaaat gtttcatttc aatatcaaag tgatgcatcc
    54301 ttcacattga aagatgtttc ttttaatata cctaaaggtc agtggacatc tattgttggt
    54361 cataacggtt ctggaaaatc tacaattgcc aagttaatga ttggcataga gaaagttaaa
    54421 tctggagaaa ttttttataa taatcaagct ataactgatg ataattttga aaagttaaga
    54481 aaagacatag gaattgtatt tcagaatccg gataatcaat ttgttggttc aattgtaaaa
    54541 tacgatgtgg catttggact cgaaaatcat gcggttccat atgacgaaat gcatagaaga
    54601 gtcagcgaag cacttaaaca agttgatatg ttagaacgtg cagattatga acctaatgca
    54661 ttatcggggg gacagaagca gcgtgtggct atagcaagtg tattagcact taacccctct
    54721 gtcattatat tagatgaggc gacttctatg ttagatcctg atgcacgtca aaatttattg
    54781 gatttagtga gaaaagttaa atcagaacat aatattacaa tcatttctat tacgcatgat
    54841 ttatctgagg cgatggaagc agatcatgtt atcgttatga ataaagggac tgtctataaa
    54901 gaaggcacag cgactgaaat tttcgacaat gcagaagagt taacaagaat aggtctagat
    54961 ttgccattcc caatcaaaat aaatcaaatg ctgggacacc aaacatcatt cttaacttat
    55021 gaagggctgg tggatcaact atgactatac ggtttgacaa tgtaagttat acctatcaaa
    55081 aagggacacc atatcagcat caagctattc atgatgttaa tacagaattt gaacaaggta
    55141 aatattacgc catcgttgga caaacgggta gtggtaaatc aacgttgata caaaatatta
    55201 atgcgctgtt aaagccgact actgggacag ttacagttga tgacattact attacacata
    55261 agaccaaaga taaatatatt agacctgtaa gaaaaagaat tggaatggta tttcaatttc
    55321 ccgaatctca attatttgag gacacagtag agcgtgaaat gatatttgga cctaaaaact
    55381 ttaaaatgaa tttagatgaa gccaaaaact atgcccatcg tctgttgatg gatttaggct
    55441 tttcaagaga tgtaatgtca caatcaccat ttcaaatgtc aggtggacaa atgcgtaaaa
    55501 tagcgattgt atcgatattg gcaatgaatc ctgatattat cgtggttgat gaacctacag
    55561 caggacttga tccacaaagt aaacgacaag taatgagatt actaaagtca ctacaaacag
    55621 atgaaaataa ggcaattatc ctaatttcac atgatatgaa tgaagtcgcg cgttatgcag
    55681 atgaagtcat cgttatgaaa gaaggtagta tagtgtcgca aacatcacca aaagagctct
    55741 tcaaagataa aaaaaaattg gcggattggc atattggttt gccagaaatt gttcagttac
    55801 aatatgactt tgaacaaaaa tatcaaacaa aattaaaaga tattgcctta actgaagaag
    55861 catttgtaag cttgtataag gagtggcaac atgaaaaata aattgattat agggcgatat
    55921 ttaccaatta attcttttgt tcatcatctt gatccaagag caaagttaat gtttgttttt
    55981 ctttttatta tattaatatt tttctgtcat tcaccattaa catatctttg ggtgtttgca
    56041 cttatcttat tcattatgag attagcaaaa attcaattat ggttcctaat caaaggttta
    56101 acaccaatat ttttcttttt aatcttcaca ttaatgatgc atatattttt aactaaaggt
    56161 ggatatgtat tagttgaatg gcatggcatc acgattgaaa ctaatggtat tttagaaggg
    56221 ctatatatat cactgcgttt aattgggatt gtaatgattg caacaattat gacactatct
    56281 acaagtccaa ttgatttaac agatgcgttt gaaagattac ttgcaccact aaagatgttt
    56341 aaattaccag ttcatcaatt aagtatgata atgtccattg cgttacgatt catcccgacg
    56401 ttaatggatg agttagataa aatcattttg gcgcaaaagt cgcgtggttc tgaaataagt
    56461 tcagggaaca ttgcaacacg tattaaatca tttattccat tactagtgcc actattcatc
    56521 tcagctttcc aacgcgccga agaattagcg gtcgcaatgg aagttagggg ttatgatgcc
    56581 aatgttaaga gaacgagcta cagacagctt aaatggcaat tgagagatac gatatcttta
    56641 accatgatta tcccaattgc aattatttta ttcgtattaa aatattcagg agtgtaaatt
    56701 catgcgtata ttagtagaaa ttgcgtatca aggaaataat tttctaggtt ttcaaattca
    56761 acaaaatggt cgaacggtac agcaacaatt tgaaaagcta ttacaacgca tgcataaaag
    56821 acatgtgaga atccatcctt caagtaggac tgatagaggt gtacatgcga tacagcaata
    56881 ctttcatttc gacacagagc tgaatatacc tatgtcacaa tggcaatatg caatgaaccg
    56941 aacgttacct gatgatattt atgtcaacaa tgtagttaca gtcgatgacg attttcattg
    57001 tcgttatgat tgtgtaggta aacgttatcg ctacaaagta tatcaagcac aacaccgcga
    57061 tccatttcaa agtggtttga aaacatttat tcctgaaacg ttggatttag gtaaaatgaa
    57121 cagagcggcg caacaattca taggtacaca tgattttacg ggattttgtt cgcaaaaaac
    57181 tgaagtagaa agcaaagtta gaacattata ccaaagtgaa atcgtaaaga cagatgatgg
    57241 ctttgattat attgtcactg gatctggatt tttatataac atggtgcgtg tcttagttgc
    57301 atttttaata gaagttggaa aagggcgaca tgaagtatct gatgtaccaa aacttcttga
    57361 atcaaagaat cgtaagaatg tacctttcac tgcaccggct gaaggattat atttagagaa
    57421 aatttattta gatgaaaatg agttattaaa agactttggt aacgatatca aaatacatcg
    57481 taaaaaatca ttacaaaatg actgattgcc attgacaaaa agcttgtgaa attataagat
    57541 tattaacggt attgttttat atccacccca cgataagccc cggaaactta ttgtgttaca
    57601 agatatataa gcagaaacga acaacagtta acaaaataaa tgaaattaaa cgttttaaaa
    57661 atgaaacaaa tgaaatcatc tattaggtta tgaaactgtt tatagcttga atagaagcat
    57721 ttatttttta ggaggacaat tattatgcgt caaacattta tggcaaatga atcaaacatt
    57781 gagcgcaaat ggtatgttat cgatgctgaa ggccaaacat taggtcgttt atcatcagaa
    57841 gtagcatcta tcttacgcgg taaaaataaa gtaacttaca caccacacgt tgatactggt
    57901 gattatgtaa tcgttattaa tgcatcaaaa atcgaattta ctggtaacaa agaaactgac
    57961 aaagtttact accgtcactc aaatcaccca ggtggtatca aatcaatcac tgctggtgaa
    58021 ttaagaagaa ctaacccaga acgtttaatt gaaaactcaa ttaaaggtat gttaccaagc
    58081 actcgtttag gcgaaaaaca aggtaaaaaa ttatttgtat atggtggcgc tgaacatcca
    58141 cacgctgcac aacaaccaga aaactacgaa ttacgtggtt aattagaagg aggaaatgac
    58201 tttggcacaa gttgaatata gaggcacagg ccgtcgtaaa aactcagtag cacgtgtacg
    58261 tttagtacca ggtgaaggta acatcacagt taataaccgt gacgtacgcg aatacttacc
    58321 attcgaatca ttaattttag acttaaacca accatttgat gtaactgaaa ctaaaggtaa
    58381 ctatgatgtt ttagttaacg ttcatggtgg tggtttcact ggacaagctc aagctatccg
    58441 tcacggaatc gctcgtgcat tattagaagc agatcctgaa tacagaggtt ctttaaaacg
    58501 cgctggatta cttactcgtg acccacgtat gaaagaacgt aaaaaaccag gtcttaaagc
    58561 agctcgtcgt tcacctcaat tctcaaaacg ttaattgtcg gacgatatat acaaaacacc
    58621 tcgatattat gtcgaggtgt ttttttgcgt ttttgcggcg aatatggaat gtgtagaata
    58681 taaatgaatt tttaccttcc caccataaaa gatgaagaac catgaaatgt ggagaacaat
    58741 aaatagttga tattctgtta tttttttgga agtgaagtga tttgaatact ttactctaaa
    58801 cgataaaagg ttaaaaaaac aacaaaaaga aaatattact gtcataattg gaaatacacc
    58861 gtattctgct ggtcaatcta gtgtaaataa tgataataaa aaaatgaata tgaaaaatta
    58921 aatgcaaaaa tagcaaatac ctatgcaaat ttttcaacag caaatttaaa aaaatcatta
    58981 tatgattcgt atatacaggc ttttcgatgg gcaactgata gagttggaaa aaaggtatta
    59041 taggatttgt aacaaatgga tcatttattg atagtcaaag cactgatgga tttagaaaag
    59101 ttttatacga tgaatttaac tatctgtaca taattaattt gagaggcgac caacgaacac
    59161 agggtgaaaa atcaagaaaa gaaggtggaa aaatatttgg atcaggaagt agagcaccga
    59221 tagctatatc tattttagtt aaagatggaa gttacaatca cgatatctat tataatgaca
    59281 ttggtgaata tttaactaga gagcaaaaat tagatacttt aatgaaacat caatcaattg
    59341 tgaatttgaa aagtttgaat gttttacccg ataaaaacaa tgattggatt aatcaacgag
    59401 acataaacta tgagaattat ttaccaatgt atgattccaa ggatattgaa aattctatat
    59461 atttagatca atttaatgga gtaaattcag caagagataa ttgggtaaca aatttttcga
    59521 atgaaaaagc attagtaaat gcaaaattac ttgtagataa ctataattct gaaatagata
    59581 gattaattga tatcttagat tcaagagaac gtataaattt agtgaacaaa gatgaaacat
    59641 ttattagttg gacacgagga cttacccaaa aattttctaa aggaaaaaat atttcaatta
    59701 atccagagag aattgttaaa tttatgcata gaccatttac aaaaaaatgg atagtgtacg
    59761 acaaaaatat tatggaaatg ccaagtagat attataatat aatggagaat actggacagg
    59821 tgatttatat acaaggacag ggaatgaata aagaattctc ggctatgatc acggacatat
    59881 tacctaattt ccaatttatt ggtaatggca aaggctttgc tacatacaaa ggtaaagata
    59941 gtttgaggct tgtagataat atttctaatt cttttaaaaa gaaaataaat cttaattcag
    60001 aagagatagt atattatata tatgcaattt tgcatcataa atattatgtg aacaaatatt
    60061 caagtgattt gagtaaaggt tttccgcgaa taccgatatt aaaggatgta tatggatttg
    60121 ttgaaattgg tagggagtta gtggaattac atttaaatta tgaaaaacaa ctcaattggg
    60181 atggtgtaga aataatttat aataatatga atccgaatta taaagttgaa aaatgaagca
    60241 ccctaaaaag ggcgtgttgg atacagtaat atacaatacc gatattacag ttactaacat
    60301 tccaaataaa gtatataatt atattgtgaa tggcaagcct gctattgaat ggattataga
    60361 tcagtacaac gtaagcatcg acaagaaatc tggaatatta gatgatccta atgaatttag
    60421 tgaagatccg aattacattt taaatctgct tttatcagtt attactgtaa gtatgaaaac
    60481 tcttggatta atagataaat taccagattt tgaagtatta gattaaggag aggatatatt
    60541 gacaaatact atcaaaaaaa taatttattt tgatgaacta tcagcaatgg atttattaca
    60601 gatagagaag aaaggtaacc ttactaaaac agttcagttg atgcatgatg aaaaaggtag
    60661 tgcaagtggt gaatctaaag tgaacatgga atttggtaag caaagcgctg taaaacaaat
    60721 ttttgattct ttacttggga tgtcagcgag tgcttcggct agtgcaggta taaatggaaa
    60781 tataactgga gagcgagtag ccaaaacaat tttagagaat acattaatat atgatttttt
    60841 ggatacagta gagtttagaa agaaaaaaac tattattgat atttcagagg ggtttaaact
    60901 aaatattcaa caagatacga tgacatattt cgcgactatt acccccatag caagtatgtt
    60961 tgaaggatct aaacatctag acgaggagga gctatcattt gatgtttcaa aaatgaatga
    61021 agcgattaga agtattaaag gctattttga attaatagga acaagaggga atgaaaacag
    61081 agtttttaga tttaatatag aatcttttaa aaataactat agaatacaag atttaaaatt
    61141 tatgaattta acactctact cagtacttgt tggtgaaata agcaaagaaa atctaaatta
    61201 cgataaagag tttgatctaa atcaaggtgc caaacctagt ggaattggaa agtttaaggg
    61261 aattagatca gaagcaacta aagaagataa atatcaagtg tatgatgtta ttcttgcagg
    61321 agtgaaataa atggaaattc atgtttatac gggaccgctt tgtggattta atgaatatat
    61381 taattctgaa gttaaagaat atcaccgctt catggaatta atcagagaat acaatatcat
    61441 agtgcgagca agtgatacat ttggaactca tgatgttgat ggtatttttg atactgagat
    61501 agaaaatctt gtgatatttt caaacgactt tgcttctgta acaactcatg ttattactaa
    61561 ttttataaat atcgttactt taggtagaaa tattaagaaa atctatattc agaatcctcc
    61621 aaaaagagta tttcaatcac taaatagttt tcacgattct gaaataat
//
LOCUS       DALAAR010000007        59657 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000007 DALAAR010000000
VERSION     DALAAR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 59657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.007"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            252..743
                     /gene="lspA"
                     /locus_tag="O2458_000458"
     CDS             252..743
                     /gene="lspA"
                     /locus_tag="O2458_000458"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCZ3886347.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            743..1660
                     /locus_tag="O2458_000459"
     CDS             743..1660
                     /locus_tag="O2458_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ3886348.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2059..2586
                     /gene="pyrR"
                     /locus_tag="O2458_000460"
     CDS             2059..2586
                     /gene="pyrR"
                     /locus_tag="O2458_000460"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCZ3886349.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2804..4111
                     /locus_tag="O2458_000461"
     CDS             2804..4111
                     /locus_tag="O2458_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCZ3886350.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4139..5020
                     /locus_tag="O2458_000462"
     CDS             4139..5020
                     /locus_tag="O2458_000462"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCZ3886351.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5038..6312
                     /locus_tag="O2458_000463"
     CDS             5038..6312
                     /locus_tag="O2458_000463"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCZ3886352.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6314..7414
                     /gene="carA"
                     /locus_tag="O2458_000464"
     CDS             6314..7414
                     /gene="carA"
                     /locus_tag="O2458_000464"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCZ3886353.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7407..10580
                     /gene="carB"
                     /locus_tag="O2458_000465"
     CDS             7407..10580
                     /gene="carB"
                     /locus_tag="O2458_000465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCZ3886354.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10690..11382
                     /gene="pyrF"
                     /locus_tag="O2458_000466"
     CDS             10690..11382
                     /gene="pyrF"
                     /locus_tag="O2458_000466"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCZ3886355.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11382..11993
                     /locus_tag="O2458_000467"
     CDS             11382..11993
                     /locus_tag="O2458_000467"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCZ3886356.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12023..12235
                     /locus_tag="O2458_000468"
     CDS             12023..12235
                     /locus_tag="O2458_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886357.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            12672..13073
                     /locus_tag="O2458_000469"
     CDS             12672..13073
                     /locus_tag="O2458_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886358.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13336..15033)
                     /locus_tag="O2458_000470"
     CDS             complement(13336..15033)
                     /locus_tag="O2458_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCZ3886359.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15308..15931
                     /gene="gmk"
                     /locus_tag="O2458_000471"
     CDS             15308..15931
                     /gene="gmk"
                     /locus_tag="O2458_000471"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCZ3886360.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15931..16149
                     /locus_tag="O2458_000472"
     CDS             15931..16149
                     /locus_tag="O2458_000472"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCZ3886361.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16365..17564
                     /gene="coaBC"
                     /locus_tag="O2458_000473"
     CDS             16365..17564
                     /gene="coaBC"
                     /locus_tag="O2458_000473"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCZ3886362.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17564..19972
                     /gene="priA"
                     /locus_tag="O2458_000474"
     CDS             17564..19972
                     /gene="priA"
                     /locus_tag="O2458_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCZ3886363.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            20478..21431
                     /locus_tag="O2458_000475"
     CDS             20478..21431
                     /locus_tag="O2458_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886364.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(21594..21797)
                     /locus_tag="O2458_000476"
     CDS             complement(21594..21797)
                     /locus_tag="O2458_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ3886365.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            22096..22584
                     /locus_tag="O2458_000477"
     CDS             22096..22584
                     /locus_tag="O2458_000477"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ3886366.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            22577..23512
                     /locus_tag="O2458_000478"
     CDS             22577..23512
                     /locus_tag="O2458_000478"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCZ3886367.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            23509..24816
                     /gene="rsmB"
                     /locus_tag="O2458_000479"
     CDS             23509..24816
                     /gene="rsmB"
                     /locus_tag="O2458_000479"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCZ3886368.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            24819..25913
                     /gene="rlmN"
                     /locus_tag="O2458_000480"
     CDS             24819..25913
                     /gene="rlmN"
                     /locus_tag="O2458_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCZ3886369.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            25920..26663
                     /locus_tag="O2458_000481"
     CDS             25920..26663
                     /locus_tag="O2458_000481"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCZ3886370.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            26660..28654
                     /gene="pknB"
                     /locus_tag="O2458_000482"
     CDS             26660..28654
                     /gene="pknB"
                     /locus_tag="O2458_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCZ3886371.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            28882..29757
                     /gene="rsgA"
                     /locus_tag="O2458_000483"
     CDS             28882..29757
                     /gene="rsgA"
                     /locus_tag="O2458_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCZ3886372.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            29758..30402
                     /locus_tag="O2458_000484"
     CDS             29758..30402
                     /locus_tag="O2458_000484"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCZ3886373.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            30409..31050
                     /locus_tag="O2458_000485"
     CDS             30409..31050
                     /locus_tag="O2458_000485"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCZ3886374.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(31431..31619)
                     /locus_tag="O2458_000486"
     CDS             complement(31431..31619)
                     /locus_tag="O2458_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCZ3886375.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            32062..32436
                     /locus_tag="O2458_000487"
     CDS             32062..32436
                     /locus_tag="O2458_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ3886376.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            32451..34097
                     /gene="fakA"
                     /locus_tag="O2458_000488"
     CDS             32451..34097
                     /gene="fakA"
                     /locus_tag="O2458_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCZ3886377.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            34287..36347
                     /gene="recG"
                     /locus_tag="O2458_000489"
     CDS             34287..36347
                     /gene="recG"
                     /locus_tag="O2458_000489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCZ3886378.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            36550..37122
                     /gene="fapR"
                     /locus_tag="O2458_000490"
     CDS             36550..37122
                     /gene="fapR"
                     /locus_tag="O2458_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCZ3886379.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            37127..38113
                     /gene="plsX"
                     /locus_tag="O2458_000491"
     CDS             37127..38113
                     /gene="plsX"
                     /locus_tag="O2458_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCZ3886380.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            38106..39032
                     /gene="fabD"
                     /locus_tag="O2458_000492"
     CDS             38106..39032
                     /gene="fabD"
                     /locus_tag="O2458_000492"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCZ3886381.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            39025..39759
                     /gene="fabG"
                     /locus_tag="O2458_000493"
     CDS             39025..39759
                     /gene="fabG"
                     /locus_tag="O2458_000493"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCZ3886382.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            40002..40235
                     /locus_tag="O2458_000494"
     CDS             40002..40235
                     /locus_tag="O2458_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCZ3886383.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            40351..41082
                     /locus_tag="O2458_000495"
     CDS             40351..41082
                     /locus_tag="O2458_000495"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCZ3886384.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            41229..44795
                     /gene="smc"
                     /locus_tag="O2458_000496"
     CDS             41229..44795
                     /gene="smc"
                     /locus_tag="O2458_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCZ3886385.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            44795..46045
                     /gene="ftsY"
                     /locus_tag="O2458_000497"
     CDS             44795..46045
                     /gene="ftsY"
                     /locus_tag="O2458_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCZ3886386.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            46032..46364
                     /locus_tag="O2458_000498"
     CDS             46032..46364
                     /locus_tag="O2458_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCZ3886387.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            46390..47757
                     /gene="ffh"
                     /locus_tag="O2458_000499"
     CDS             46390..47757
                     /gene="ffh"
                     /locus_tag="O2458_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCZ3886388.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            48192..48467
                     /gene="rpsP"
                     /locus_tag="O2458_000500"
     CDS             48192..48467
                     /gene="rpsP"
                     /locus_tag="O2458_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCZ3886389.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            48655..49158
                     /gene="rimM"
                     /locus_tag="O2458_000501"
     CDS             48655..49158
                     /gene="rimM"
                     /locus_tag="O2458_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCZ3886390.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            49158..49895
                     /gene="trmD"
                     /locus_tag="O2458_000502"
     CDS             49158..49895
                     /gene="trmD"
                     /locus_tag="O2458_000502"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCZ3886391.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            49998..50348
                     /gene="rplS"
                     /locus_tag="O2458_000503"
     CDS             49998..50348
                     /gene="rplS"
                     /locus_tag="O2458_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCZ3886392.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(50592..53198)
                     /gene="yfhO"
                     /locus_tag="O2458_000504"
     CDS             complement(50592..53198)
                     /gene="yfhO"
                     /locus_tag="O2458_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCZ3886393.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            53599..54483
                     /gene="ylqF"
                     /locus_tag="O2458_000505"
     CDS             53599..54483
                     /gene="ylqF"
                     /locus_tag="O2458_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCZ3886394.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            54467..55234
                     /locus_tag="O2458_000506"
     CDS             54467..55234
                     /locus_tag="O2458_000506"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCZ3886395.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            55343..56509
                     /gene="sucC"
                     /locus_tag="O2458_000507"
     CDS             55343..56509
                     /gene="sucC"
                     /locus_tag="O2458_000507"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCZ3886396.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            56531..57439
                     /gene="sucD"
                     /locus_tag="O2458_000508"
     CDS             56531..57439
                     /gene="sucD"
                     /locus_tag="O2458_000508"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCZ3886397.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            57666..58784
                     /locus_tag="O2458_000509"
     CDS             57666..58784
                     /locus_tag="O2458_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ3886398.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            58812..>59657
                     /gene="fmhC"
                     /locus_tag="O2458_000510"
     CDS             58812..>59657
                     /gene="fmhC"
                     /locus_tag="O2458_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ3886399.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKL"
BASE COUNT        21897 a         8200 c        11352 g        18208 t
ORIGIN      
        1 tttttaatta gaaaatatct tactactgtc tttttaggga tttatgttcc agcctgcttt
       61 cctaattttt aatgtcatct taaatatgat aaatgaataa ttaagttcat atttaatgtc
      121 aaaacatagt agtttatcaa gtattgagtt agtaacatta gatttaatgt aatatgctta
      181 ctttttttat tagcaggtgt aagctataat ataaagagtt gtctcatgga cgattgattg
      241 gaggaacgaa aatgcacaaa aaatatttta ttggcacttc cattttaata gcagtatttg
      301 tcgttatatt tgaccaagtt actaaatata ttatagctac tacaatgaaa attggagatt
      361 catttgaagt gataccgcac tttttaaaca taacatcaca tcgaaataat ggtgctgcat
      421 ggggaatatt gagtggaaaa atgacatttt tctttattat taccattatt atattaatag
      481 ccttagtata tttctttatt aaagatgctc aatataattt gtttatgcaa gttgctatta
      541 gtttactttt tgcaggtgca cttggaaact ttattgatag aattttaaca ggagaagttg
      601 ttgactttat tgatacaaat atttttggtt atgattttcc aatatttaat atcgcagatt
      661 caagtttaac aattggtgta atattaatta ttattgcctt attaaaggat acttccaata
      721 aaaaggagaa ggaggttaag taatggagac ttatgaattt aacattacag ataaagaaca
      781 aacaggtatg cgtgtagata agttgctgcc tgaattaaat aatgattggt ctcgtaacca
      841 gatacaagat tggattaaag caggtttagt cgttgcaaac gataaagttg ttaaatctaa
      901 ttataaagtg aaacttaatg atcatatagt tgtcactgaa aaagaagtgg ttgaagctga
      961 tattctacct gaaaatttaa atttagatat ttattatgaa gatgacgatg ttgcagttgt
     1021 atataaaccg aaaggcatgg tagttcatcc atcaccaggg cattatacca atacattagt
     1081 taatggttta atgtatcaaa ttaaaaattt gtcaggtatt aatggagaaa ttcgtccagg
     1141 tattgttcac cgtatagata tggatacttc tggtttatta atggttgcta aaaatgatat
     1201 tgctcatcgt gggcttgtag aacaattaat ggataaatct gttaaaagaa aatatatcgc
     1261 tttagttcac gggaatattc ctcatgatta cggtacaatc gatgcgccaa ttggtagaaa
     1321 caaaaatgat cgtcaatcta tggctgttgt tgatgatggt aaggaagcag tgacacattt
     1381 taacgtacta gaacatttta aagattatac gcttgttgaa tgtcaacttg aaacaggacg
     1441 tacgcatcaa atccgtgttc acatgaaata tattggcttc ccattagttg gtgatccaaa
     1501 gtatggaccg aaaaagacat tggatattgg tggtcaagct ctacatgctg gacttattgg
     1561 attcgaacat ccagtaacaa gtgaatatat tgaaagacat gctgaattac cacaagactt
     1621 tgaagattta ttagatacaa ttcgaaaaag agatgcataa ttgtgtcgtg ctataattac
     1681 gataacatta ttatgtaaac tataagtgtt tatcgttttc gtttttttaa atgaaaacac
     1741 attataaaat agattaaggt taacattttt actgagtatg gttaattttt caatatagat
     1801 tgacaaagcg cctgattagt attatcctta taacagttta agtacaacat aaagagtctt
     1861 taattgagcg tccagagaga cgtcaaagac atgaaaatat agatatatac taaaatgatc
     1921 ataacacttt cacatatgca agaataagta gtaatactac gattcttcta tgtaaatagg
     1981 ctgattgtat atactaaacc tcatgtcgat attaaagaca tgaggttttt tgtttctaaa
     2041 ttttgaaagg gtgagaatat gtctgaacgt atcataatgg atgatgccgc aatacaacgt
     2101 acagtgacga gaatcgctca cgaaattttg gagtataaca aaggtactga taatttaatt
     2161 cttttaggta tcaaaacaag aggtgaatat ttagcgaatc gtatacaaga taaaattcat
     2221 caaattgagc aacaacgtat acctactgga acaattgata ttacatactt tagagatgat
     2281 atagagcaca tgtcatcact tacgacaaaa gacgcaatag acatcgacac agatattaca
     2341 gataaagtag tcatcattat tgacgatgtg ctgtatactg gtcgaacggt tcgtgcttca
     2401 cttgatgcta ttttgctaaa tgctagacct attaaaattg gtttagctgc tttggttgat
     2461 cgaggacatc gtgagttacc aattcgagca gattttgttg gtaaaaatat acctacttct
     2521 aaagaggaaa cggtaagtgt ctatttagaa gaaatggatc aaagaaatgc agttataatt
     2581 aaataatacc ccttttaatt cagtacgaga gactgaaaaa ggtgggggag ttgaatgata
     2641 aataagtttt catatgcttg tttatctaat aataccctca tgttcgacaa ctataagtca
     2701 atttctatgg acgaaaatga cttgtagcaa cttgtcacac tttttgtctc tttacatata
     2761 ctgatgtaaa gagatttttt tattatcgga ggaaatgaat attatgcaaa atgatgaaat
     2821 gtttgaacga acagtaaaac ccgtactaga tgtaaatgaa aaaccacaac cagcgcaatg
     2881 ggcattttta agcttacaac atttatttgc gatgtttggc gcaacagtac tagtaccatt
     2941 cttaacagga ctaccaatat cagcagcgtt actagcttcg ggaatcggta cattacttta
     3001 tatcttaata acgaaggcgc aaataccagc atacttggga tctagctttg catttatcac
     3061 gccaattatc acgggattaa gtacgcatag cttaggagac atgcttgtag cattattcat
     3121 gagtggtgtt atgtacgtca tcatcgggat tctaatcaaa ttaagtggga cagcatggtt
     3181 aatgaaatta ttaccaccag ttgttgttgg accagtcatc atggtaattg gtttaagctt
     3241 agcgcctact gcagtcaata tggcaatgta tgaaaatcct ggagatatga aaggttacaa
     3301 tatcagtttc ttaattgttg caatgataac cttgcttgta acaatagtcg ttcaaggatt
     3361 ttttaaagga ttcctatctt taattccagt tcttgtaggt attatcgtag gttatgtggt
     3421 agcgattttt atggggattg tgaaatttga tgcaattatg tcagcaaaat ggatagattt
     3481 ccctcatatt tatctgccat ttaaagatta tgtaccttca tttcacttag gacttgtact
     3541 tgtaatgatt ccgattgtgt tcgtaacagt aagtgaacat attgggcacc aaatggtatt
     3601 gaataaaatc gtaggtagaa acttctttga aaagccagga cttgataaat caatcattgg
     3661 tgatggtgtt tctacaatgt ttgccagtat tattggtgga ccaccaagta caacatacgg
     3721 tgaaaatatc ggtgtattag cgattaccag aatatacagt atttacgtca ttggtggtgc
     3781 agcagttata gcaattgttt tagcattcat tggtaagttc actgcattaa tttcttctat
     3841 acctacacca gttatgggag gagtatctat attacttttc ggtattattg cagcaagtgg
     3901 cttaagaatg ttagttgaaa gcaaagtaga ttttgcgaac aatcgaaatt tagttatagc
     3961 ttctgtaatt ttagttgtag gtatcggtaa tttagtattt aacttaaaag aaattggtat
     4021 caaccttcaa attgagggga tggcattagc tgcactttca ggaattattt tgaacttaat
     4081 cttacctaaa gagaaaaaac aaaacaatta agatttacaa attaaggagg gcgcttttat
     4141 gaatcattta ttatcaatgg aacatttatc tacagatcaa atatacaaac ttatccaaaa
     4201 ggcaagtcaa tttaaatctg gtgaacgtca actaccaaac tttgaaggga aatatgtcgc
     4261 aaatttattc tttgaaaatt ctactcgaac aaaatgtagt tttgaaatgg cagaacttaa
     4321 gctagggtta aaaacgatta gctttgaaac atcaacatca tctgtttcaa aaggtgaatc
     4381 tttatatgac acatgtaaaa ctttagaaag tattggctgt gatttattag tcattagaca
     4441 tccgtttaat aactactatg aaaaattagc gaatattaac atcccaattg cgaatgctgg
     4501 tgatggtagt ggacaacatc caacacaaag tttacttgat ttaatgacga tatatgaaga
     4561 atatggatat tttgaaggct tgaatgtatt gatttgtgga gacattaaaa attcacgtgt
     4621 cgcacgtagt aattaccata gtttaaaagc attaggtgca aacgtaatgt ttaatagccc
     4681 aaatgcttgg attgatgatt ctttagaagc accttatgta aatatagatg atgttataga
     4741 aacagtagat atagttatgt tattaagaat tcaacatgaa aggcatgggc ttgcagaaga
     4801 aactagattt gcagcagatg attatcatca aaagcatggc ttaaatgaag tacgctataa
     4861 caaattacaa gaacatgcta ttgttatgca tccggcacct gtgaatagag gagtagaaat
     4921 acaaagcgat ttagtagaag cttcaaaatc aagaattttt aagcaaatgg aaaatggcgt
     4981 ttacttaaga atggcagtca ttgatgaatt attaaaatag gtaaggggac gaaaatgatg
     5041 aaattaatta aaaacggtaa agtattacaa aatggcgaat tacaacaagc agatatttta
     5101 attgatggta aggtaattaa acaaattgca cctgcaattg aaccaagcaa tggtgttgac
     5161 atcatagatg cgaaaggtca ctttgtgtca cctggatttg tcgatgttca tgttcattta
     5221 cgtgaacctg gtggtgaata taaagagaca attgaaactg gtactaaagc tgctgctaga
     5281 ggcggattta caactgtatg tccaatgcct aacacaagac cggtaccaga ttctgtagaa
     5341 cattttgaag ctttacaaaa attaatcgat gacaatgctc aagtacgtgt attaccttat
     5401 gcttcaatta caacacgtca attaggtaaa gaattggttg atttcccagc actagtaaaa
     5461 gaaggtgcct ttgcgtttac agatgacggt gtaggagtac aaactgcaag catgatgtat
     5521 gaaggcatga ttgaagctgc aaaagtaaac aaagccatcg tagcacactg tgaagataat
     5581 tcattaatct atggtggtgc aatgcatgaa gggaaacgca gtaaagagtt aggtatacca
     5641 ggtattccaa acatttgtga atctgttcaa atcgcaagag atgtactatt agctgaagca
     5701 gcaggttgtc attatcatgt atgtcatgtt tctactaaag aaagtgttag agtcattcgt
     5761 gacgctaaac gcgcaggcat tcatgttaca gctgaagtta caccacacca tttattgtta
     5821 acagaagatg atattcctgg taataatgcc atttataaaa tgaatccacc attgagaagt
     5881 actgaagata gagaggcttt gttagaaggg ttactagacg gtacaattga ctgtatcgca
     5941 acagaccatg caccacatgc acgtgatgaa aaagcacaac caatggaaaa agcaccattc
     6001 ggaattgttg gtagtgaaac agcattccca ttattatata cgcattttgt aaaaaatggt
     6061 gattggacat tacaacaatt agtagattac ttaacaatta aaccatgtga gacatttaat
     6121 ttagaatacg gcacattaaa agaaaatggt tatgcagatt taacaatcat tgatttagat
     6181 agtgaacaag aaattaaagg agaagatttc ttatcaaaag cagataatac accatttatc
     6241 ggctataaag tttatggaaa tccgatctta acaatggttg aaggcgaagt taaatttgag
     6301 ggggataaat aatatgcaaa gcaaacgtta tctagtgtta gaagacggtt ctttttacga
     6361 aggctaccgt ttaggatctg ataacttaac tgtaggagaa attgtattta atacagcgat
     6421 gacaggttat caagaaacta tttcagatcc atcatataca ggtcagatca ttacttttac
     6481 gtatccatta atcggtaatt atggtatcaa tagagacgat tttgaatcat tagtacctac
     6541 attaaacggt attgtagtga aagaagcgag tgcgcatcca agtaatttta gacagcaaaa
     6601 gacacttcat gacgttttag aattgcatca aattccaggg attgcaggtg ttgatacaag
     6661 aagtattacg cgtaaaattc gacaacacgg tgtgttaaaa gctggtttta ctgatcgaaa
     6721 agaagatatt gatcaacttg tcaaacattt acaacaagta gaattaccta aaaacgaagt
     6781 agaaatcgtt tcgactaaaa caccgtatgt ttcgacaggt aaggatctaa gtgtcgtact
     6841 tgtagacttt ggtaagaagc aaaatattgt tcgagaatta aacgtcagag gttgtaacgt
     6901 cacagttgta ccatatacaa ctactgccga agaaatttta gcaatggctc cagatggcgt
     6961 tatgctatca aacggaccag gtaatcctga agttgtagaa tgtgcgattc caatgattca
     7021 aggaatttta gggaaaattc cgttctttgg tatctgtcta ggacatcaac tttttgcatt
     7081 atctcaagga gcaagctcat ttaaaatgaa gtttggtcat cgtggtgcga accatccagt
     7141 taaaaattta gagactggaa aagttgatat tacgagtcaa aaccatggat atgcaataga
     7201 tatagattcg ttaaaaagta ctgatttaga agttactcat cttgcattaa atgatggtac
     7261 tgtagaaggt ttaaaacata aaacattacc agcattttct gttcaatacc atcctgaagc
     7321 aaatccagga ccgtcagatt caaactatct atttgatgat tttgtagcaa tgatgactaa
     7381 ttttaaggaa aaggagcgtc atatcaatgc ctaaacgtaa tgatatcaaa acaattttag
     7441 taatagggtc tgggccaatt atcataggtc aagcagctga atttgattat gctggaacac
     7501 aagcatgtct agctttaaaa gaagagggat atcgagttat tcttgtaaat tcaaatccag
     7561 cgacaatcat gactgataag gaaattgcgg ataaagtata tatcgaaccg ttaactcatg
     7621 attttatagc gcgaattata cgtaaagagc aacctgacgc tttacttcca actttaggtg
     7681 gtcaaacagg tttaaacatg gcgattcaac tacacgaaag tggtgtgctt caagataata
     7741 acgtccaatt attaggaact gagctaacat caattcaaca agcagaagac cgtgaaatgt
     7801 ttagaacatt aatgaatgat ttaaacgttc ctgtaccaga gagtgacatt gtaaatacag
     7861 tagagcaagc ctttaaattc aaagagcaag tgggataccc gctaattgtt agaccggcat
     7921 ttacgatggg tggtaccgga ggcggtattt gtcataatga tgaagaatta catgaaatcg
     7981 tctcaaatgg tcttcattat agtccagcaa cgcaatgttt attagaaaaa tctatcgcag
     8041 gttttaaaga aatcgaatac gaagtaatgc gtgataaaaa cgataatgcc atcgttgtat
     8101 gtaacatgga aaatattgat ccagttggta ttcatacagg cgattcaatt gttgtggctc
     8161 ctagtcaaac attatcagat gttgagtatc aaatgttacg tgatgtttca ttaaaagtta
     8221 ttcgagcttt aggtatcgaa ggtggttgta atgttcaatt agcattagat ccccattcat
     8281 tcgattatta tattatagaa gtaaatccgc gtgtatcacg ttcatcagcg ttagcttcaa
     8341 aagcaacagg atatcctatt gcaaaattag ctgctaaaat cgcggttggt ctaacattag
     8401 atgaaatgtt aaatccaatt acaggaacat cttatgcagc gtttgaacca actttagact
     8461 atgtgatttc aaaaatacca agatttcctt ttgataaatt tgaaaaagga gaacgagagc
     8521 ttggcacaca aatgaaagca acaggtgaag ttatggccat tggtcgaact tacgaagaat
     8581 cattgttaaa agcaattcga tcacttgagt atggtgtgca tcacttagga ttaccaaatg
     8641 gtgaaagctt cgatcttgat tatattaaag aacgtatttc acaccaagat gatgaacgat
     8701 tatttttcat cggcgaagca attagaagag gcacaacatt agaagaaatt cataatatga
     8761 ctcagattga ttacttcttc ttacacaagt tccaaaacat tattgatatt gagcatcaat
     8821 taaaagagca tcaaggtgat ttagaatatc ttaaatatgc aaaagattat ggatttagtg
     8881 ataaaacaat agcgcatcgc tttaatatga cggaagaaga agtatatcaa ttgcgtatgg
     8941 aaaatgatat taaacctgtt tacaagatgg ttgatacttg cgcagctgaa tttgaatctt
     9001 caacaccata ttattatggt acatacgaaa ctgaaaatga atccatagtt actgacaaag
     9061 aaaaaatctt agtattaggc tctggaccaa ttcgaatcgg ccaaggtgta gaatttgact
     9121 atgcgacagt tcacgccgtt tgggcaattc aaaaagcagg gtacgaagcg ataattgtga
     9181 ataacaatcc agaaacagtt tcaacagact tctcaatttc tgacaaatta tactttgaac
     9241 ctttaactga agaagatgtg atgaatatca ttaatttaga aaaacctaaa ggtgtcgttg
     9301 tacaatttgg aggacaaaca gcgattaatt tagcagacaa attggctaaa catggtgtta
     9361 aaatacttgg tacttcacta gaaaatctaa atcgtgctga agatagaaaa gaatttgaag
     9421 cactattaag aaaaattaac gtgccacagc cacaagggaa aacagctaca tcacctgagg
     9481 aagcattagc gaatgctgca gaaatcggat atccggttgt agtaagacct tcttatgtat
     9541 taggtggtcg cgcaatggaa attgtagaca atgacaaaga gttagaaaac tatatgaccc
     9601 aggctgtaaa agcgagtccg gaacatccgg tactagtcga tagatattta actggtaaag
     9661 aaattgaagt tgatgcgatt tgtgatggag aaacggtcat tattccagga atcatggaac
     9721 atattgaacg tgctggtgtg catagtggtg actcaatcgc tgtatatcca ccacaaactt
     9781 tgacagaaga cgagttagca acacttgagg actatactat aaaattagct aaaggtttaa
     9841 acatcattgg cttaatcaac attcaattcg ttatagctca cgatggtgtg tatgttttag
     9901 aagtaaatcc acgttctagt agaacggtac cattcttaag taaaattact gatattccaa
     9961 tggcacaatt agctatgcga gcaatcattg gggaaaaact aacagatatg ggttatcaag
    10021 aaggggttca accatatgct gagggtgtct ttgtgaaagc accagtattt agttttaata
    10081 aattgaaaaa tgttgatatt actttaggac ctgaaatgaa gtcaacaggt gaagtgatgg
    10141 ggaaagatac tacattagaa aaggcgttat tcaaagggtt aacaggtagt ggcgttgaag
    10201 ttaaagatca cggtacagta ttaatgaccg tcagtgacaa agataaagag gaagttgtta
    10261 aattggcaca acgcttaaat gaagttggct ataaaatttt agcaacgtct ggaacagcta
    10321 ataaattagc tgagtatgac atacctgcag aagtagtagg caaaattggt ggcgaaaatg
    10381 atttattaac acgtattcaa aatggtgatg ttcaaatcgt tataaataca atgactaaag
    10441 gtaaagaagt agaaagggat ggcttccaaa ttagacgtac tacagttgaa aatggtattc
    10501 catgtttgac atctttagat acagctaatg ccttaacgaa tgtaattgaa agtatgacat
    10561 ttacaatgcg tcaaatgtaa atcaatcaaa ctgtatcggt ggggctgtaa ttaaccattt
    10621 acttaaagaa gtttatatta cagcctcatt attttaatga atttcttaat ataaagggag
    10681 acatatatga tgaaagattt accaattatt gcattagatt ttgaatcaaa agaaaaagta
    10741 aatcaatttt tagatttatt tgatgaatca ttattcgtaa aagtaggtat ggaacttttt
    10801 tatcaagaag gtcctcaatt aattaatgag ataaaagaaa gaggccatga tgtattttta
    10861 gatttaaaac tgcatgatat tcctaataca gttggtaagg cgatggaagg actagctaaa
    10921 ttgaatgttg atctggtaaa tgttcatgct gctggtggcg taaaaatgat gtctgaggcc
    10981 attaaaggat taagaaaaca taatcaagat acaaaaatta ttgcagtaac acagcttacg
    11041 tcaacaacag aagacatgtt acgacacgaa caaaatatac aaacatcgat tgaagaggcc
    11101 gttttaaatt atgccaagtt agcaaatgca gctggtttag atggcgttgt ttgttcacct
    11161 cttgaaagtc gtatgttgac tgaaaagtta ggtacatcat ttttaaaagt aacaccaggt
    11221 attagaccta aaggtgcatc tcaaaatgac caacaccgta ttacgacacc ggaagaagca
    11281 agacagcttg gttcgacgca tattgtagtc ggtagaccga ttacacaaag tgacaatcca
    11341 gtcgaaagtt atcataaaat taaagaaagt tggttagtat aatggctaaa gaaattgcaa
    11401 aatcattatt agatattgaa gctgtaacat tatcaccaaa tgatttatat acatggagtt
    11461 caggtattaa atcaccgatt tactgtgata accgtgttac gttaggttat cctttagttc
    11521 gaggcgcaat ccgcgatggt ttaattaact taattaaaga acactttcct gaagtagaag
    11581 ttatttctgg tactgcaaca gctggtattc cacatgcagc ttttattgct gaaaaattaa
    11641 aattaccaat gaattatgtt cgttcatcaa ataagagtca tggtaagcaa aatcaaatcg
    11701 aaggtgctaa aagtgaaggt aaaaaagtag ttgtgataga agatttaatt tcgacagggg
    11761 gatcttcagt cacagcagtt gaagccttaa aactagcagg tgcagaagta ttaggtgttg
    11821 tagctatctt tacttacggt ttgaaaaaag cagatgatac atttagcaat attcaactac
    11881 ctttttacac tttaagtgat tacaatgaat taattgaagt agctgaaaat gaaggtaaaa
    11941 tttctagtga agatatccaa acattagttg aatggagaga caacttagca taatatagac
    12001 actagaagga ggaattcaac aaatgaatga caaaacatct aatgatttat atgggaagat
    12061 aaaacattgt aacgaattta tcaatcattc aaatgattcc aatctatcta gtagtcacga
    12121 tgtcgacgaa agttcaacga agcaaaaaca tataaaaaat aaaacaacta tagatcataa
    12181 tgatgattta tttaaacatg taaaggatat attacgtaaa caagaacaaa tttaaaaaca
    12241 aaaaagctat agtgtttaat atcaggggct gcttaaccgc atccgaatat tacaaacact
    12301 atagctttta ttatataaaa aatttattag cggataatta actttattaa aatccaacca
    12361 attaagaata cgagtaatag gactaatact ggaatcacat aatgtaacat aacgtccctc
    12421 ctttaactta attttaattg taatcaaatt tgacaataag tcaaaacatt aatacctatg
    12481 ataagtatca tttattaaca tatgtatcat atttttaatc ttgcgtaatt tttatcgtta
    12541 actatgtgat ttaatcaaca ggccaataag acgtttgtca ttcgtgcaca taacggtacg
    12601 acgcgctttt gtcattttaa ttatgttaag ataatagtag ttatagaagt tcaatctata
    12661 ggaggcatag catggatatt ccaaaaatca cgacattttt aatgtttaat aaccaagctg
    12721 aagaagctgt taaactatac acaagcttat ttgaagatag tgagattata acaatggcta
    12781 agtatggtga aaatggacct ggtgatcccg ggactgtaca acactcaata tttacattaa
    12841 atggacaagt attcatggcg attgatgcta atagtggcac agaattacca atgaatcctg
    12901 cgatttcatt atttgttaca gtaaaagata ctattgaaat ggaacgacta tttaatggat
    12961 taaaagatga aggtgccatt ttaatgccaa aaacgaatat gccaccatac agagagtttg
    13021 cttgggttca agataagttt ggagtaagtt ttcaattagc attacctgag taaaaggatt
    13081 cgcacagcgt tgaatgataa agaaacactt tttcttatgc atgcgtttta cctatgtatt
    13141 tctattttaa gtacacatta gcattttagt ttcactcatt tttaaaatca caaagataat
    13201 ttgtaactta aatgatgata cgttatttaa aaacacgata cttcgtttca atgaacgcat
    13261 taaataataa ataaacacct caccataaga aaggactact ttcttttgtg aggtgtttat
    13321 ttgtcgacaa cttgattatg attgtttcat tttttgaata agttcataat caggtgtagc
    13381 atacaaagtt ttttgattgt catatgttac aaacccaggc tttgcacctg atggtttatg
    13441 cacattttta attaatgtat aatcaacagg tatttgtcca gaattaccag cttttgaaaa
    13501 gtatcctgct aacatagccg cttccttgat tgtcgtatca cttggtgcat cattaaatat
    13561 aacgacatgt gaaccaggaa tatcttttgt gtgtaaccat gtgtgagttt ttttagcttt
    13621 tttatttgtt aaataatcat tttgcttgtt attcttacca acatatatat cgtcgccatc
    13681 agttgataca taatgttgta attgaatctg cgctttcttt ttcttagttt gatttttacg
    13741 ctgtttcata aagccttgtt ctgctaattc atctctaatt tcatcaatgt catggacaga
    13801 aatatgatgt aattgttgtt cgattgttga aaaataatct atattgtctt tcgtcaattg
    13861 aatttgatgt tgtaattcac gttctctcgt tttcatacga ttatattgtt tataataata
    13921 ttgagcattt gctgatgggg attttgtagg atttaaagga atgacaactt cttcattcgt
    13981 ataataattc aatgccgtca cttctttatc gccttgctta attcgatata tattagcagt
    14041 gatcaattca ccatataact gttcagtatc tttattttta gactgctcat attcttcaat
    14101 caacttcgct aatttatttt gatatttgta caactgctgt tgaacaaatc gaactaaatc
    14161 attcgcacgt tgtttaacgc gttcacgttc gccacgcgca tcataaaaac gatcaagcaa
    14221 atcatttaat gaatcgtatg taactgtatc atcattaaat tgatttaact ttataaaata
    14281 gaaatcctct ttacctgttt catgattttt atgaaaaata ggagtaggtg gtaacttggt
    14341 ttctgccatt acttcgtcaa atgcttctgg taatgttgat gaagtaataa attgacgacg
    14401 actaacgatt tcattcgtaa ttaaagggct aaatccttca aactgattca ataattgttt
    14461 agcaatatta cctgcgttaa aatcgatata tttcaacacc tctgcacctg taatatcata
    14521 cggatttatt ttgtgctgag taggtggtgc ttcataatta aatcctggca ttactgtacg
    14581 atagtgattc gtatttggtg ttaagtgttt aaatccttca attattttgc gattttcatc
    14641 tactaaaatt aagttactat gtttacccat aatctcaagg atgacagtgc ggtaaatagt
    14701 atcgccaatt tcatctttac tctttatatc gatttcaatg cgacgatcat taccaatttg
    14761 cttaatcgat tcgataatac caccttctaa gtgttttcta aaaacacgcg caaacatggg
    14821 tggattaaat ggattatcat attttttagt agtcaattgt aatcttgaaa agtttggatg
    14881 gattgacaat aacaattgat ggttttgtct attttgacgt acaaccatta gtatcgtgtc
    14941 attatcaggt tgattgattt tgtgaacgcg tcctgttgtt aaaaattgta gagactcaac
    15001 cattttcttt gtaaataagc catcataagc cataatttat cttccacctt cttcatatca
    15061 ttcgtttcat tttaacatga ttgtaacttt tcagtatagt tattgaacca tacattatgc
    15121 catacatttt acattatcta aaagtttgca attcaattta tttaattaaa attaatcgtg
    15181 caatataacg atattgttag acttaataga aattatggca tgcaatttca aatatgctat
    15241 acaatataaa gaacaatgtg atatcatatt taaataatag aagattagct tagagaggtc
    15301 gtaaggcatg gataatgaaa aaggattgtt aatcgtttta tcaggaccat ctggagtagg
    15361 taaaggtact gttagaaaac gaatatttga agatccaagt acatcatata agtattctat
    15421 ttcaatgaca acacgtcaaa tgcgtgaagg tgaagttgat ggcgtagatt acttttttaa
    15481 aactagggat gcgtttgaag ctttaatcaa agatgaccaa tttatagaat atgctgaata
    15541 tgtaggcaac tattatggta caccagttca atatgttaaa gatacaatgg acgaaggtca
    15601 tgatgtattt ttagaaattg aagtagaagg tgcaaagcaa gttagaaaga aatttccaga
    15661 tgcgctattt attttcttag cacctccaag tttagaacac ttgagagagc gattagtagg
    15721 tagaggaaca gaatctgatg agaaaataca aagtcgtatt aacgaagcgc gtaaagaagt
    15781 tgaaatgatg aatttatacg attacgttgt agttaatgat gaagtagaac ttgcgaagaa
    15841 tagaattcaa tgtattgtag aagctgagca cttaaaaaga gagcgcgtag aagctaagta
    15901 tagaaaaatg attttggagg ctaaaaaata atgttaaatc cacctttaaa ccaattaacg
    15961 tcacaaatta aatcaaagta tttaattgca acaactgcag cgaaaagagc gcgtgaaatt
    16021 gatgaacaac ctgaaactga attattaagt gaatatcatt catttaaacc agttggtaga
    16081 gcgttagaag aaattgctga cggtaaaatt cgccctgtta tttcaagtga ttattatggt
    16141 aaagaatagt ttcgaccatt aaaaatatgt gaagctagac gtacatcaaa tacgtcaagg
    16201 catatccaaa tcaaacgcac ctatgtaggt gtgttttttt agtttatcca atgattgatg
    16261 ttataataat actaaatttg tatctataaa aaagtaatga gcatttgtgc gcatatgatg
    16321 atgtaaagcg taaatgtcat agtgatattt aagggagatt gaatatgaag aaaatattat
    16381 tagccgttac aggtggcatt gcggcatata aagcaattga tttgacaagt aagttaacac
    16441 aatctgggta tgaagttcgc gttatgttaa cgaatcacgc acaaaaattt gtgacaccat
    16501 tagcatttca agcaataagt cgaaatgctg tttatacaga tacttttata gaagaaaatc
    16561 cttcagaaat acagcatatt gcattaggtg attgggcaga tgcaatcatt gttgcacctg
    16621 caacggcaaa tacaattgca aaattgagtg taggtattgc tgatgatttg gtgacatcaa
    16681 cgttgctagc aacagagaca ccgaaattta ttgcgcctgc tatgaatgtg catatgtatg
    16741 aaaataaacg tacgcagcaa aatattaata ttttaaaaga agatgggtat cattttatcg
    16801 aaccaggaag cggatttcta gcatgtggtt atgttgctaa aggacgtatg gaagaaccgc
    16861 ttcaaatcgt ttctgtaatt gatgctcatt ttcaaaatag taatcgttta gctaatagtt
    16921 catttcaaga taaacgcgca ttggttacag caggaccaac tattgaagtt atcgatccag
    16981 tcagatttgt atccaatcgt tcttctggaa aaatgggcta tgcaatagct gaagcattgc
    17041 gaaatcgagg agctatcgtg acgttagttg ctggtcctac aacactagag gatccaaaag
    17101 atattgaagt tattcatgtt caaagtgctg aagaaatgtt tgaacaagtg acaagccgat
    17161 ttgacgaaca agatattgtt gtaaaagcag cagccgtatc tgactataca ccggttgatg
    17221 tattagaaca taagatgaaa aagcaggatg gtgatttgtc agtatctttt aaacgtacta
    17281 aagacattct taaatattta ggtgaacata aaacatcaca gtatttaata ggctttgcag
    17341 cagagactga agatattgaa aattatgcac aacaaaaatt acgcaagaaa aatgcagatg
    17401 tgattatttc aaataatgtt ggggatatgt ctatcggatt tagttctgat gataatgaat
    17461 tgacaatgca ttttaaaaat aatgaaaaag taaatatcaa gaaaggaaaa aaagtagtat
    17521 tagctgcaca aattttagat gaactagaaa ctaggtggca ataatgatag cgaaagtcat
    17581 agtcgatgtc gcgtcgaaga gcgttgacta taaatttgat tatataattc ccgaacaact
    17641 cgaatctgtc atccaacctg gtgtgcgtgt aattgtacct tttggaccaa gaacgattca
    17701 aggttatgta atggaagtaa cagcagaacc tgatgcacaa cttgacgttt cgaagttaaa
    17761 aaaaatcata gaagtgaaag atatacaacc agaattaaca tcagaattaa tagctttaag
    17821 tgagtggatg ggttcaactc atgtcattaa acgtatttct atgctagaag tgatgcttcc
    17881 gagtgctatt aaagcgaagt ataaaaaagc atttaagatg aaagatgaca tagagctacc
    17941 ttcagcttta ttacaaaaat ttgataagca tggttactat tattataaag atgcgcaaaa
    18001 aaataatgat attcaattgc ttatgaagtt gttaaaagat gatatcgttg aagaaaaaac
    18061 gattctcaca caaaatataa ctaaaaaaac caagcgtgct gttcgtgtca ttgaagggta
    18121 tcatcctgat gaagtattag ctaagttgga gaaagttatt aaacaatacg atttgtatgc
    18181 ttacttgtct gaagaacaac ataaaacaat atttttaact gatattgagg atatgggctt
    18241 ttcaaaatcc agtttagatg gacttatcaa aaaaggttat gttgaaaaat atgacgcggt
    18301 tgttgaaaga gacccattta aagatcgtgt tttcgaacaa gaatcaaaac agcaattaac
    18361 agaagaccaa tataaagcat atgaagcgat taaagctaaa attgtaagcc aagagcaaga
    18421 aacattttta cttcatggtg tgacgggatc aggtaaaaca gaagtatatt tacaaacgat
    18481 agaagatgtt ttaagccaag gaaaacaggc gatgatgtta gttcctgaaa tcgctctaac
    18541 accgcaaatg gttttacgct tcaaacgtcg atttggtgat gacgttgctg tattacattc
    18601 tggcttatct aatggggaac gttatgatga atggcaaaaa attagggatg gtcgtgcgag
    18661 agtaagtgtt ggtgcaaggt caagtgtgtt cgcacctttc aaaaatttag ggttaatcat
    18721 cattgatgaa gaacatgaat ctacatataa acaagaagat tatccgagat atcacgctag
    18781 agaaattgcc caatggcgaa gtgaatatca tcactgtcca gtcattttag gaagtgcaac
    18841 accatgtctt gaaagttatg cacgagctga aaaaggcgtt tatcatttgc tatcattacc
    18901 aaacagagtg aaccaacaag ctttacctga aattgatata gtagacatgc gtgaagaatt
    18961 gagtgaaggt aatcggtcaa tgttttcaaa agatttacgt gaagccatac aattaagatt
    19021 agatcgacag gaacaagttg ttttattttt aaatcgacgt ggttatgcat cgtttatgtt
    19081 atgtcgggat tgtggatatg taccgcaatg tccaaactgt gatatttcat taacgtatca
    19141 taaaacgaca gacttattaa aatgtcacta ttgtggttac caagagacgc caccgaatca
    19201 atgtccaaat tgtgagagtg aacacattcg acaagtaggt actggtactc agaaagttga
    19261 agaactattg caacaagaat ttgaagatgc gcgcataatt aggatggatg tagatacaac
    19321 ctcaaagaaa ggtgcacatg aaaagttatt gactgaattc gaaaaaggta acggtgacat
    19381 tttactaggt actcagatga ttgcgaaagg attagattat ccaaatatta ctttagttgg
    19441 tgtgctgaat gcagatacaa tgttaaattt acctgatttt cgggcgagcg aacgtactta
    19501 tcaactatta acgcaagtgg ctggtagagc tggtcgtcat gaaaaggcag gtcaagtcat
    19561 cattcaaacg tataatccag atcattattc aatattggat gttcaaaaaa atgattattt
    19621 aacattttat cgtcaggaaa tggaatatcg taaattagga aagtatccac cgtattatta
    19681 tttgattaat ttcacaatct cacataaaga aatgaagaag gttatggtag catcgcagca
    19741 tgttcataaa attttattac agcatttaac agaaaaagcg cttgtactag gtccatctcc
    19801 ggcagcactt gcgagaatca acaatgaatt tagattccaa attttagtga aatataaaag
    19861 tgaacctgga ttattacaag ccattcagtt tttagatgac tattaccatg aaaaatttat
    19921 aaaagaaaaa ttagcattga agattgatat tgatccacag atgatgatgt aacattacta
    19981 attattagaa acaagttcag tatgtacgag tatttgaacc agtgtttgaa tatttacttt
    20041 aagtacagaa aaagggcaga atatacaact gttaactatt taaattagca gtttatattc
    20101 tgtcttttta tatggcttta taacttacgt gattttggtt tgataaggaa tttattagta
    20161 ttttcattta cgaattctga ttttaatgca agcgccatac ttactgatga ataaaagtac
    20221 taatgattaa acaaactatt tttaaatggc aaattgaaat agtgcgaatt ttaaacttat
    20281 aaattaatgg ctataaacta ttttatatct atctatgttt taactactta aattattaga
    20341 caaaacaatt ttcttaaatt atcaattaaa gtatttaaaa atattgatgg ttaatgtaaa
    20401 atatcaatat aaataagttt tttaaattta tgtatgttta tttgattcaa acaaaataac
    20461 ttaagaggag aaagtttatg aaaaagacac tgggatgttt acttttaatt atgcttttag
    20521 tcgtagcagg ttgttctttt ggtgggaatc ataaattatc atcaaagaaa tcagaagaat
    20581 caaaacaaga aactgtaaaa aaagaatcgg aagaagagaa agatccagat ttagagaaat
    20641 atgaagaaat agagaagaaa atgaaaggaa ttaaagatgc gccatctctt gataagttgg
    20701 atccattaat gacagaaaag tcgtttacga atagtaaagg gattcaagga tggaaagatt
    20761 acaaagaatt aatgggtaaa gtggaacttg cagattatag atttactaaa gattcaaaag
    20821 gatcttcaat aaaagatgtt gatgcattct ttaaaggtaa gaaaggtata aaaaggaaag
    20881 tgattgaaac acacgatgat gtaaaacaag ttgattattg gtatgtagat ccagatggaa
    20941 agaaaattgg caattcaaac acacctgttt tttacgcaga aattatgaca aaatataaag
    21001 atggaaagtt agtttatgca tcagtcgaac caggatctta cgtaatacat aaagatgatg
    21061 caattaaata tgacgattat tctaagttaa aaaaattaag tcagctaact aaacttgatc
    21121 atccaaaacc agttccatat agcgtagctc aaatcaaatc tttcggagta cctttaacaa
    21181 gcgtttcatt tatgacacat ggatcaaagg atactaaaga tgaagtgttg ccggcattgg
    21241 cctatttcac tttttcacca aaaaattatg aagacaagtc taatccagat ccaaaagttt
    21301 taaatttagt acatatggat ttcttaaatg catctagtga ttttggtaac gcacattttg
    21361 ttgttttaag taaatatatt aaagagtatg aatcaaacta tgaaacagcg tcagatgatt
    21421 ctttaaaata gtatttactg tgtgaaaaat aaatagtgta ctacattaaa taatcgcaat
    21481 aataatcccg ataaacaatc agcattactg cttatcacat agagttcgta ataactataa
    21541 ctctatgatt cgcaaataat aaatgattgt catcgggatt tatttttatc aatttataaa
    21601 gtgacattac cttgttcatc agcaggtttg aaaacagtaa tcactgcact aataattgct
    21661 aaaatgtgtg ggatacctgt ccaacagaat attaagtgta gaataccttg catattcttg
    21721 ccggcataaa atttatgaat accaaaacta cctaagaaca atgctaataa aatataaata
    21781 actttgttta cttgcatttc tttccctcca gttgaattgc ttataatgac attagcttct
    21841 ctttttatta tacccacttt tagttcaaac attctagttt aagcattccc aatcatctaa
    21901 atttcagtta ttcaatcctt acaataaatt taggattaca tttcagttgc attgtattat
    21961 tttacgtgtg aaatatacgt aatgaatcac atgacaatct tcaaattgaa aaatatacat
    22021 tctatgatgt aaggtcgcat ttttaatata tttacgttat aatagtttga tgaattttat
    22081 tgaggagtaa gagttatggc gattaaaaag ttagtaccag catcgcatcc tattttaacg
    22141 aaaaaagcgc aagcagttaa aacatttgat gattcgttaa aaagattatt acaagattta
    22201 gaagatacaa tgtatgcaca agaagctgct ggcttatgtg cacctcaaat taatcagtca
    22261 ttgcaagtgg caattattga tatggaaatg gaaggattat tacaacttgt taatccgaaa
    22321 attattagtc aatcaaatga aacaataaca gacttagaag gttcaattac attgccagat
    22381 gtttacggcg aagtgacaag aagtaaaatg atagttgtcg aaagttatga cgtcaatggg
    22441 aacaaagttg aactaactgc acatgaagat gtagcaagaa tgattttgca tattatagat
    22501 caaatgaacg gtatcccttt tacagaacgt gcggaccgta ttttaacaga taaagaagtg
    22561 gaggcatatt ttataaatga ctaaaataat atttatgggt acaccagact tttcaacaac
    22621 tgttttagaa atgcttattg cagaacatga tgtcattgca gtcgtaacgc aaccagatcg
    22681 acctgttgga cgtaaacgtg ttatgacacc accaccagtt aaaaaagttg caatgaaata
    22741 tgatttacct gtatatcaac ctgaaaaatt aagtggatca gaagaattag aacaattgct
    22801 tcaattagat gtagatttaa ttgtaactgc tgcttttgga caattattac ctgaatcatt
    22861 gttggcatta ccaaatcttg gggcaattaa tgtacatgca tcattgttac cgaagtatag
    22921 aggtggtgca ccaattcatc aggcaattat cgatggtgaa caagaaaccg gcataacaat
    22981 tatgtatatg gttaaaaaat tagatgcggg caatattatt tcgcaacaag caattaaaat
    23041 agaagaaaat gataatgtcg gtacgatgca tgataaatta agtgtattag gggcagattt
    23101 attaaaagaa actttaccat ctattataga gggcacaaat gaaagcgtac ctcaagatga
    23161 tacgcaagca acatttgctt ccaatattcg acgcgaagat gagcgaatta gctggaataa
    23221 accaggaaga caagtgttta atcaaattcg tggattatca ccatggccag ttgcttatac
    23281 aactatggat gacactaact tgaaaatata cgatgctgaa ctcgttgaga ctaataagat
    23341 aaacgagcct ggaaccatta tagaaacgac taaaaaagcc attattgttg ctacaaatga
    23401 taatgaagct gttgcaatta aagatatgca attagctggg aaaaagagaa tgttagctgc
    23461 caattattta agtggtgcgc aaaacacact agtagggaag aaacttatat gatagaaaac
    23521 gtgagaagtc ttgcttttga cacgattcaa gatatattaa atgaaggtgc gtatagtaac
    23581 ttgcgtatca atgaagtgtt gtcagaaaat gaattaaatg caatggataa ggctttattt
    23641 acagaaattg tctacggaac cgttaaaaga aaatatacgt tagattttta tttaaagcct
    23701 tttgtgaaaa caaagattaa ggcatgggtt aggcaattat tatggatgag tatttatcaa
    23761 tatgtttatt tagataaagt tccaaatcat gccattatta atgaagcagt tgaaatagca
    23821 aaagaacgcg gtggctatca taatggtaat gtcgtaaatg gtattttacg tacaatgatg
    23881 cgtagtgact tacctgattt taatgaaatt gcagatccta aaaaaagaat ggcaatcgag
    23941 tatagtatgc cgaagtggat tatagatcat tgggcaacac attatggtct cgaagaaact
    24001 gaaacaattt tacagtcatt tttagaaacg acatcaacaa ctgtgcgtgc caacctgacg
    24061 cgagcatcat tagatgatat tattgaaaag ttgcaagacg aaggttatga cgttgaaaaa
    24121 gatcatgact taccttattg tctccatata ggaggacaac caattattca ttctcgttca
    24181 tttaaagatg gattcgtttc aattcaagat aaaagctcaa tgtttgttgc acacattatg
    24241 aatgtagacc gacatgatca cgtattagat gcatgtagtg cacctggcgg taaagcttgt
    24301 cacattgctg aagttttaat gccagaaggg caagttgacg cttcagatat acatgatcac
    24361 aaaatagact taattaattt taatataaaa aaattacgat taacaaatat taaagctttt
    24421 caacatgatg cgacaaaacc ttatgataaa acatacgata agatacttgt tgatgcacca
    24481 tgtagcggat taggtgtaat gagacataag ccggagatta agtatactca aagcaaacaa
    24541 catattgagt cactagttga attacagctt gaaatattgg aaaatgtaaa aaacaatgta
    24601 aaaataggtg gcgaaatcat ctattcaaca tgtacaattg agcaactaga aaatgaaaac
    24661 gtgatttata cgtttttgaa aaataataaa aacttcgaat ttgaaccgtt tcaacatccg
    24721 ataactggag agttggtcaa aacgttacaa atcatgccgc aagactttaa ttcagatgga
    24781 ttctttatca ctaagataaa aagaaaggac aattaggaat gataactgct gaaaagaaaa
    24841 agaagaataa atttcttcca aattttgaca agcaatcaat atattcattg cgatttgacg
    24901 aaatgcaaaa ctggctcgtt gaacaagggc aacaaaaatt tcgagcgaaa cagatttttg
    24961 aatggttata tcaaaaaaga gtagattcga ttgatgaaat gacgaactta tcgaaagact
    25021 tacgacagct tttaaaagat aactttactg ttacaacttt aacaactgta gtaaaacaag
    25081 aaagtaaaga cggtacaatt aaattcttat ttgaattaca agatggctat acaattgaaa
    25141 ctgttttaat gagacatgat tatggaaatt cagtatgtgt aacgacacaa gtaggttgtc
    25201 gcatcggatg tacgttttgt gcttctacac ttggcggctt aaaaagaaac cttgaagctg
    25261 gcgaaattgt ttcacaagtt ttaacagttc aaaaagccct tgatgctaca gaagagcgcg
    25321 tatctcaaat tgtcataatg ggtatcggtg aaccatttga aaattatgat gaaatgatgg
    25381 actttttaag aatcgtcaat gatgataaca gtttaaatat tggtgcacgt cacattacag
    25441 tatcaacatc aggtatcatt cctagaatat atgactttgc ggatgaagat attcaaatta
    25501 attttgctgt aagcttacac gccgcaaaag atgaagtgcg atcacgcttg atgccaatta
    25561 accgtgcata taatgttgag aagttaatcg aagcaattca atattatcaa gaaaaaacaa
    25621 atcgtcgtgt tacttttgaa tatggtctgt ttggtggtgt gaatgaccaa ctagaacatg
    25681 caagagaatt agcacattta ataaaaggct taaactgcca tgttaactta attcctgtca
    25741 accatgttcc agaaagaaat tatgtgaaaa cggctaaaaa tgatatcttt aaatttgaaa
    25801 aagaattaaa gagactagga attaatgcca caatacgtcg tgaacaaggt tcggatattg
    25861 acgcagcttg tggtcaatta agagcaaagg aacgacaagt agaaacgagg taaagacaaa
    25921 tgctagaggc acaatttttt actgatactg gacaacatag agataagaat gaagatgcgg
    25981 gtggtatttt ttataatcaa actaatcaac aacttttagt tctgtgtgat ggtatgggtg
    26041 gccataaagc aggagaagtt gcaagtaaat ttgttacaga tgagttgaaa tcccgttttg
    26101 aagcggaaaa tcttatagaa caacatcaag ctgaaaattg gttgcgtaat aatataaaag
    26161 atataaattt tcagttatat cactatgcac aagaaaatgc agaatataaa ggtatgggta
    26221 caacatgtgt ttgtgcactt gtttttgaaa aatcagttgt gatagcaaat gtcggtgatt
    26281 ctagagccta tgttattaat agtcgacaaa ttgaacaaat tactagtgat cactcatttg
    26341 ttaatcatct tgttttaacg ggtcaaatta cgccggaaga agcatttaca catccacaac
    26401 gtaatattat tacgaaggtg atgggcacag ataaacgtgt gagtccagat ttgtttatta
    26461 agcgattaaa tttttatgat tatttattat taaattcaga tggattaact gattatgtta
    26521 aagacaatga aattaagcgt ttgttagtaa aagaaggtac aatagaagat catggtgatc
    26581 aattaatgca attggcatta gataaccatt cgaaagataa cgttactttc atactcgcgg
    26641 ctattgaagg tgataaagta tgataggtaa aataataaat gaacgatata aaattgtaga
    26701 taagcttggc ggcggtggca tgagtaccgt ttatcttgct gaagatacga tacttaacat
    26761 taaagttgca attaaggcga tttttatacc acctagagaa aaagaagaaa cattaaaacg
    26821 ttttgaacga gaagtacata actcatcaca gctatcacat caaaatatag taagtatgat
    26881 cgatgttgat gaagaagatg actgttacta cttagtaatg gaatatatcg aaggtccgac
    26941 tttgtctgag tatattgaaa gtcatgggcc attaagtgtt gacacagcga ttaattttac
    27001 gaatcaaata ttggatggca ttaaacatgc gcatgatatg cgtattgtac atagagatat
    27061 taagccacaa aatatattaa ttgacagcaa taaaacgttg aaaatatttg attttggaat
    27121 tgctaaagct ttaagtgaga cgtctttaac tcagactaat catgtgttag gtactgtgca
    27181 gtacttttcg ccagaacaag caaaaggtga ggcaacggat gaatgtacag atatttattc
    27241 tataggtatt gtgttatatg aaatgcttgt tggtgaacca ccctttaatg gagaaactgc
    27301 agttagcatt gcgattaaac atattcagga ttctgtgcca aatgtgacaa cagatgtacg
    27361 taaggatatt ccgcaatctt taagtaatgt cattttacgc gctacagaaa aagacaaagc
    27421 gaatcgttac aaaacaattc aagaaatgaa agatgatttg agtagtgttt tacatgaaaa
    27481 tcgagcgaat gaagatgtct atgaactcga taaaatgaaa acgatagcgg tacctttgaa
    27541 aaaagaagat ctagcaaagc atattagtga acataagtcg aatcaaccta aacgtgaaac
    27601 gacgcaagta cctattgtaa atgggcctgc tcatcatcag caattccaaa agccagaagg
    27661 tacggtgtac gaaccaaaac ctaaaaagaa atcaacacga aagattgtgc tcttatcact
    27721 aatcttttcg ttgttaatga ttgcacttgt ttcttttgtg gcaatggcaa tgtttggtaa
    27781 taaatacgaa gagacacctg atgtaatcgg gaaatctgta aaagaagcag agcaaatatt
    27841 caataaaaac aacctgaaat tgggtaaaat ttctagaagt tatagtgata aatatcctga
    27901 aaatgaaatt attaagacaa ctcctaatac tggtgaacgt gttgaacgtg gtgacagtgt
    27961 tgatgttgtt atatcaaagg gccctgaaaa ggttaaaatg ccaaatgtca ttggtttacc
    28021 taaggaggaa gccttgcaga aattaaaatc gttaggtctt aaagatgtta cgattgaaaa
    28081 agtatataat aatcaagcgc caaaaggata cattgcaaat caaagtgtaa ccgcaaatac
    28141 tgaaatcgct attcatgatt ctaatattaa actatatgaa tctttaggca ttaagcaagt
    28201 ttatgtagaa gactttgaac ataaatcctt tagcaaagct aaaaaagcct tagaagaaaa
    28261 agggtttaaa gttgaaagta aggaagagta tagtgacgat attgatgagg gtgatgtgat
    28321 ttctcaatct cctaaaggaa aatcagtaga tgaggggtca acgatttcat ttgttgtttc
    28381 taaaggtaaa aaaagtgact catcagatgt caaaacgaca actgaatcgg tagatgtacc
    28441 atacactggt aaaaatgata agtcacaaaa agttaaagtt tatattaaag ataaagataa
    28501 tgacggttca actgaaaaag gtagtttcga tattactagt gatcaacgta tagacattcc
    28561 tttaagaatt gaaaaaggaa aaacagcaag ttatattgtt aaagttgacg gtaaaactgt
    28621 agctgaaaaa gaagtcagct atgatgatgt ataaatataa ttgaagtaaa tgtaccgagg
    28681 tttctatttg gaagtctcgg tatttttatg ttgagattgc ggtagtttta aaatgcttca
    28741 tgtcatcata tacaggtgag cattgtctat caattctgta tcactctcac aatatttata
    28801 tcatttgaaa tgatcattta tatattaatt ttccagtctt tgatataata aatgcagtaa
    28861 aattaatcga gaggtgccat tttgaagaca ggtcgaatag tgaaatcaat tagtggggta
    28921 tatcaagtag acgttaatgg cgaacgtttc aatacaaaac cacgaggatt atttagaaag
    28981 aaaaaatttt caccggtagt tggtgatata gtggaatttg aagttcaaaa cattaacgaa
    29041 ggttatattc atcaagtgtt tgagcgggaa aatgagttga aaagaccacc tgtaagtaat
    29101 atagatacac tagtaattgt aatgagtgct gtcgagccaa atttttcaac gcaattatta
    29161 gatcgatttt tagttattgc acattcgtat cagttaaatg cgagaatttt ggtgactaaa
    29221 aaagataaaa caccaattga aaagcagttc gaaataaatg agttgttgaa aatatatgaa
    29281 aatattggct atgagactga atttattgga aatgatgatg atcgaaaaaa aattgtagaa
    29341 gcttggccag ctggacttat agtacttagt ggtcaatcag gtgtcggtaa gtccactttc
    29401 ttaaatcatt atcgtccaga acttaatctt gagacaaatg atatatcaaa atcattaaat
    29461 cgaggaaagc atactacaag acatgtcgaa ctattcgaac gtcaaaacgg ttatattgca
    29521 gacacacctg gattcagtgc tttagatttt gatcatatag ataaagatga aataaaagat
    29581 tattttcttg aattaaatcg atatggtgaa acatgtaagt ttaggaattg taatcatatc
    29641 aaagaaccta attgtaatgt taagcatcaa ttagagatag ggaatattgc gcaatttaga
    29701 tacgaccatt atttacaact atttaatgaa atttcaaata gaaaggttag atattaaatg
    29761 acaaaactat atccatcatt attatctgtt gattttttgg atttacaaca tgaattaaaa
    29821 cgacttgaag aagcaggcgt cgacggagtt cattttgatg ttatggatgg tcaatttgtg
    29881 cctaatatat ctattggttt accaatatta gatgcagtaa gaaaaggcac aacattacct
    29941 atagacgtac atttgatgat tgaaaatcca gaaaagtata ttgcatcatt tgcagaacat
    30001 ggtgccgata tgatttcaat tcatgtcgaa tcaacgcctc atattcatcg tgctattcaa
    30061 atgattaaac atttagataa aaaagctggt gtagtaatta atcctggtac accaatatca
    30121 caaattgaac ctattttaga cattgttgat tatgtactag tgatgacagt taacccaggg
    30181 tttggtggtc aatcatttat tgatcaatgc gtagaaaaaa tagcgggtct taatgctatt
    30241 aaaatggaac gtcaattaaa ctttgatatt gaagttgatg gaggcgtaaa taccgataca
    30301 gcgaaagttt gtgttgaaaa tggtgctaca atgctagtaa caggttcatt tttctttaaa
    30361 caagaggatt ataaaaaagt cacacaagca ttgaaaggtt gagtgtatat gcatataaat
    30421 ttattatgtt ctgatcgaca cttgccgcaa gatatttggg ccaaaagtaa tgaaggtaaa
    30481 tggggcggcg ttgatagagg tgctttgatt ttattgaagc atcaaattat cccttttttc
    30541 tcagtgggag actttgattc agtcagtaaa gaagaacgcc aacttctaac agaacagtta
    30601 caaatcaaac cagttcaagc tgaaaaagct gatacggatt tagctttagc ggttgataaa
    30661 gctgttgcac ttggatttga tagtattaca atttatggtg caacaggcgg acgattagat
    30721 cacttttttg gggcaattca gttattattg aaaaaagcat attataaaca tgatgttcat
    30781 atagaagtta tcgatcaaca aaataaaatt gaattattgc ctaaaggtca acatacagtt
    30841 gaaaaagata agagttatcc gtacatttca tttataccga tgactgatga tgtagaactt
    30901 tctctagcag gttttaaata taatttagct agacaaatgc ttaatatagg ttctacttta
    30961 actatttcaa atgaaattga gtctttgcaa gcgaaagtaa ctgtacatga tgggttgatt
    31021 ttgcaaatta gaagtacaga tttaaattaa gtatattcaa atttgtgagg atttttaata
    31081 aagtgcatga gttatttaat aaagtgacgt ttatagatag aatttataac ataataatag
    31141 atgaatcacg ctgtaattaa tgaagattat ggcgtgattt attattgaaa ggaaaattgt
    31201 aggtcctagt acattgattt atgtgccaaa atcgatattg aacccataag tccaatttga
    31261 cagagaacca ttattaaacg tattaaccaa attatattga tagttcgaat ttcactatga
    31321 accattatgt attgtttggt aacaaaaaaa gaactaagag gttgagctct tagttcttaa
    31381 gtaaatgtgt cctccacata aggtcacatt ttttatgtgc aatatattta ttaaactcta
    31441 gttactttac cagattttaa agcacgtgca gaaacccaaa cttttttagg tttaccgtca
    31501 actaggattc taactttttg aaggttagcg ttccatctac gtttagtaga gtttaaagcg
    31561 tgtgaacgtc tgttaccagt cgaagcttta cgacctgtta cgaaacattg tttacccata
    31621 tgagtacctc ctttaataaa tataaataca cataactact tatatactta attaagatag
    31681 catagtttca tttgaaaaac aatgaataat tttcacataa aagtcaaaaa tactgagttt
    31741 tgtgatataa ttgtagactg tgaagttatg tagtatgata ttttagagaa ataagtgaat
    31801 gaaacattta aaatttttat ttaatgatac tacatctatt aaaagaaaac gcctatagat
    31861 aatcgtcatg agtgtaaatt cagtttactc aaaatataat aggtgattta agcgtaagtc
    31921 attctgataa gttgtaccat tgatatttta taaaaataat caatgaaact taaagcgttt
    31981 atgactacac taagtggtaa aattttaaaa gtgattaggg aaaacgaata ataaactttg
    32041 aaatttagga gggacaaaga tatgacatta gagatttcaa atgattacgg taaaattgat
    32101 atttcaaacg aagtgattgc ttcggttgta ggtggaaagg ccgttgaatg ttatggtatt
    32161 gtaggtatgg catctagaca acaagttaga gatggtattg cggaaatact aggacatgaa
    32221 aattatgcta aaggcatcaa agtaactgaa aataatggcg tagtggatat agatatgtac
    32281 attattgtaa gttacggtgt gaaaatatct gaagttgcca ataatgtaca atcaacagtg
    32341 aaatatactt tggaaaaatc acttaatgta tcagtaaatt caatcaatat atatgtacaa
    32401 ggtgtacgtg tgaataatac aggcaagaaa gcttaggagg acaacttgaa atgattagca
    32461 aaattaatgg taaattattt gccgatatga ttatacaagg ggcacaaaat ttatctaaca
    32521 atgcagattt ggtagattct ttgaatgtgt atccagtgcc agatggtgat acaggaacaa
    32581 atatgaatct tactatgact tcaggtcgcg aagaagtaga gaataatttg tcgaaaaata
    32641 tcggcgaatt aggtaaaaca ttctcgaaag gtttactaat gggtgcaaga ggtaactctg
    32701 gtgtcatctt gtcacaatta ttcagaggat tttgtaaaaa tattgaaagt gaatctgaaa
    32761 ttaattcaaa attgttagct gaaagttttc aagctggtgt tgaaacggca tataaagctg
    32821 ttatgaaacc agttgaaggt acaatactta cagttgcaaa agatgctgcg caagctgcaa
    32881 tagaaaaagc aaataatact gaagattgta tagaattaat ggagtacatt attgtaaaag
    32941 ccaatgaatc acttgaaaac acaccaaact tattagctgt acttaaagaa gttggtgttg
    33001 ttgatagtgg cggtaaaggt ttgttatgcg tttacgaagg attcttaaaa gcgcttaaag
    33061 gtgaaaaagt tgaagccaaa gttgcaaaga tagataaaga tgaatttgta catgatgaac
    33121 atgatttcca tggtgtaatt aatactgaag atattattta tggctattgt actgaaatga
    33181 tggttcgttt tggaaagaat aaaaaagcct ttgatgaaca agaattcagg caagatatga
    33241 gtcaatttgg tgattcttta ttagtcatta atgatgaaga aattgtgaaa gttcacgtgc
    33301 ataccgaata cccaggtaaa gtgtttaatt atggtcaaca atatggtgaa ttaattaaac
    33361 ttaaggttga aaatatgaga gaacagcacc gtgaagtgat tcgaaaagaa cagcacacag
    33421 ctaaaccgaa aatggaaacg gttgaaacag caattattac tatttctatg ggtgaaggta
    33481 tttcagagat atttaaatca atgggtgcca cacatatcat tagtggtgga caaacgatga
    33541 atccttctac agaagatatc gttaaagtca ttgaacaatc aaaatgtaaa cgtgcaatta
    33601 ttttaccgaa taataaaaat atcttaatgg caagtgaaca agcagcgagt attgttgatg
    33661 cagaagctgt tgttattcca acgaaatcta ttcctcaagg tataagcgca ctattccaat
    33721 atgatgtgga cgcaacactt gaagaaaata aagcgcaaat ggctgattca gtaaataacg
    33781 ttaaatctgg ttcattaacg tacgctgttc gtgatacgaa aattgatggc gttgagatta
    33841 aaaaagacgc gtttatgggc ttgattgaag ataagattgt aagcagccaa agtgatcaat
    33901 taacaacggt tactgagttg ttaaatgaga tgttagcaga agatagtgaa atattgactg
    33961 tgattattgg tcaagatgca gagcaagcag ttacagataa catgataaac tggatcgaag
    34021 agcaatatcc agatgtagaa gtggaagttc atgaaggtgg acaaccaatt tatcaatatt
    34081 tcttttcagt agaataaaaa tttaaaataa aaaactacca atgataaatc atcagttggt
    34141 agttttttat tttgctattt tagtgatatt gcgggttaaa agtatcgttc tcgagttgct
    34201 aacaatgtca tgttcaactt agtcatgata aaataaataa catactaaat gatacgtaaa
    34261 atcaaataaa acataggtga tttattttgg ctaaagtaaa cttaatagaa agtccatatt
    34321 ctcttttaca attaaaaggt ataggtccta agaaaataga agtattgcaa caactaaata
    34381 ttcatacagt ggaagatctt gttctttatt tgccaactag atatgaagat aatacagtga
    34441 ttgatttgaa tcaagcagaa gatcaatcta acgttacgat agaaggacaa gtatatacag
    34501 ctccagtagt tgcatttttt ggaagaaata aatcaaaatt aaccgttcat ttaatggtaa
    34561 ataatattgc tgtcaaatgt atttttttca atcaaccgta tttaaaaaag aaaatcgaat
    34621 taaatcaaac tataactgtt aaaggtaagt ggaatagggt taaacaggaa attactggta
    34681 atagggtttt ctttaattca caagggacac aaactcaaga aaacgcagat gttcaattag
    34741 aaccagtcta tcgtattaag gaaggtatta aacaaaagca aatacgagac caaattagac
    34801 aagcgttaaa tgatgtgaca attcatgaat ggttaactga tgaactaaga gaaaaatata
    34861 aattagagac cttggacttt actttgaaca cattacatca tcctaaaagt aaagaggatt
    34921 tattacgtgc tcgtagaacc tatgcattta ctgaactgtt tttattcgaa ttacgtatgc
    34981 aatggctaaa tagattagaa aagtcatctg acgaagcaat tgaaattgat tatgacatag
    35041 accaagttaa atcatttatt gatcgtttac cttttgaact aactgaagca cagaaatcca
    35101 gtgttaatga aatttttaga gatttaaaag caccaatacg tatgcatcga ttacttcaag
    35161 gtgatgtagg ttcaggaaaa acagtagttg ctgcaatttg tatgtatgcg ttaaaaactg
    35221 ctggttatca atcagcattg atggtaccaa ctgaaatttt agcagagcaa catgctgaaa
    35281 gtttaatggc tttatttgga gattctatga acgttgcatt gttaactggg tcagtaaaag
    35341 gtaagaaacg aaagatactt ttagaacaac ttgaaaatgg tacgattgat tgtttaattg
    35401 gaacccatgc tttgattcaa gatgatgtga ttttccataa tgttggttta gtaattacag
    35461 atgaacaaca tcgatttggt gtgaatcaac gccagctttt aagagaaaaa ggtgcaatga
    35521 cgaatgtgtt atttatgaca gcaacgccga taccaagaac actagcaata tcagtttttg
    35581 gtgagatgga tgtgtcttca attaaacaat taccaaaagg tcgtaaacct atcattacta
    35641 cttgggcaaa gcatgagcaa tacgataaag ttttgatgca aatgacctca gagttgaaaa
    35701 aaggtcgtca agcatatgtc atttgcccgc taatagaaag ttctgagcat ctcgaagatg
    35761 ttcaaaatgt tgtcgcattg tacgagtctt tacaacagta ttatggtgtt tcccgtgtag
    35821 ggttattgca tggtaagtta tctgccgatg aaaaagatga ggtcatgcaa aagtttagta
    35881 atcatgagat aaatgtttta gtttctacta ctgttgttga agtaggtgtt aatgtaccga
    35941 atgcaacttt tatgatgatt tatgatgcgg atcgctttgg attatcaact ttacatcagt
    36001 tacgcggtcg tgtaggtaga agtgaccagc aaagttactg tgttttaatt gcatccccta
    36061 aaacagaaac aggaattgaa agaatgacaa ttatgacaca aacaacggat ggatttgaat
    36121 tgagtgaacg agacttagaa atgcgtggtc ctggagattt ctttggtgtt aaacaaagtg
    36181 gattgccaga tttcttagtt gccaatttag ttgaagatta tcgtatgtta gaagttgctc
    36241 gtgatgaagc agctgaactt attcaatctg gcgtattctt tgaaaatacg tatcaacatt
    36301 tacgtcattt tgttgaagaa aatttattac atcgtagttt tgactaattg ccatgctgat
    36361 ttgtcaattt gagtgcaaca cttcgttaat tgagtgatat gacacttgaa ctatttaaat
    36421 gtaaagtggt attttaacaa tttataaatt ttcgactaaa taatagctaa atattacagt
    36481 tatttgttga gtcggttaaa tagaaagtgt tatgatatgt gaggaatgtt taagactagg
    36541 tactaaaaaa tgaggggtga gacgttgaaa ctaaagaaag ataaacgtag agaagcaatc
    36601 agacaacaaa ttgatagcaa tcccttcatc acagaccatg aactaagcga cttatttcaa
    36661 gtgagtatac aaacaattcg tttagatcgc acttatttaa acataccaga attaaggaag
    36721 cgtattaaat tagttgctga aaagaattat gaccaaataa gttctattga agaacaagaa
    36781 tttattggtg atttgattca agtcaatcca aatgttaaag cgcaatcaat tttagatatt
    36841 acatcggatt ctgtttttca taaaactgga attgcgcgtg gtcatgtgct gtttgctcag
    36901 gcaaattcgt tatgtgttgc gctaattaag caaccaacag ttttaactca tgagagtagc
    36961 attcaattta ttgaaaaagt aaaattaaat gatacggtaa gagcagaagc acgagttgta
    37021 aatcaaactg caaaacatta ttacgtcgaa gtaaagtcat atgttaaaca tacattagtt
    37081 ttcaaaggaa attttaaaat gttttatgat aagcgaggat aaaattatgg ttaaattagc
    37141 aattgatatg atgggtggcg acaatgcgcc tgatatcgta ttagaagccg tacaaaaggc
    37201 tgttgaagac tttaaagatc tagaaattat acttttcggt gacgaaaaaa agtataatct
    37261 gaaccatgaa cgaatcgaat ttagacattg ttctgaaaag attgaaatgg aagatgagcc
    37321 tgttagagcg attaaacgta aaaaagatag ctcaatggta aaaatggctg aagctgtgaa
    37381 atctggtgaa gcagatggat gtgtgtcagc aggtaatact ggtgctttaa tgtcagctgg
    37441 tttattcatt gttggacgta ttaaaggtgt agctagaccg gctttagtag taacattgcc
    37501 aacgattgat ggaaaaggtt ttgtcttttt agacgttggt gcaaatgctg atgctaaacc
    37561 tgaacactta ttacagtatg cgcaactagg ggatatttat gctcaaaaaa ttagaggtat
    37621 tgataatccg aaaatctcat tattaaatat aggaaccgag ccagctaaag gtaatagttt
    37681 aacgaaaaaa tcatatgagt tattaaatca tgatcattca ttgaattttg ttgggaatat
    37741 tgaagcgaag acattaatgg atggcgatac agatgttgta gttaccgatg gctatactgg
    37801 gaacatggtc cttaaaaatt tagaaggtac tgcaaaatca atcggtaaaa tgttaaaaga
    37861 tacgattatg agtagtacta aaaataaatt agcaggtgca atattgaaga aagatttagc
    37921 tgaattcgct aaaaagatgg attactcaga atacggtggt tccgtattat taggattgga
    37981 aggtactgta gttaaagcac acggtagttc aaatgctaaa gctttttatt ctgcaattag
    38041 acaagcgaaa atcgcaggag aacaaaatat tgtacaaaca atgaaagaga ctgtaggtga
    38101 atcaaatgag taaaacagca attatttttc cgggacaagg cgcccaaaaa gttggtatgg
    38161 cgcaagattt gtttaacaac aatgatcaag caactgaaat tttaacttca gcagcgaaca
    38221 cattagactt tgatatttta gagacaatgt ttactgatga agaaggtaaa ttgggtgaaa
    38281 ctgaaaacac acaaccagct ttattgacgc atagttcggc attattagca gcgctaaaaa
    38341 atttgaatcc tgattttact atggggcata gtttaggtga atattcaagt ttagttgcag
    38401 ctgacgtatt atcatttgaa gatgcagtta aaattgttag aaaacgtggt caattaatgg
    38461 cgcaagcatt tcctactggt gtaggaagca tggctgcagt attgggatta gattttgata
    38521 aagtcgatga aatttgtaag tcattatcat ctgatgacaa aataattgaa ccagcaaaca
    38581 ttaattgccc aggtcaaatt gttgtttcag gtcacaaagc tttaattgat gagctagtag
    38641 aaaaaggtaa atcattaggt gcaaaacgtg tcatgccttt agcagtatct ggaccattcc
    38701 attcatcgct aatgaaagtg attgaagaag atttttcaag ttacattaat caatttgaat
    38761 ggcgtgatgc taagtttcct gtagttcaaa atgtaaatgc gcaaggtgaa actgacaaag
    38821 aagtaattaa atctaatatg gtcaagcaat tatattcacc agtacaattc attaactcaa
    38881 cagaatggct aatagaccaa ggtgttgatc attttattga aattggtcct ggaaaagttt
    38941 tatctggctt aattaaaaaa ataaatagag atgttaagtt aacatcaatt caaactttag
    39001 aagatgtgaa aggatggaat gaaaatgact aagagtgctt tagtaacagg tgcatcaaga
    39061 ggaattggac gtagtattgc gttacaatta gcagaagaag gatataatgt agcagtaaac
    39121 tatgcaggca gcaaagagaa agctgaagca gtagtcgaag aaatcaaagc taaaggtgtt
    39181 gacagttttg cgattcaagc aaatgttgcc gatgctgatg aagttaaagc aatgattaaa
    39241 gaagtagtta gccaatttgg ttctttagat gttttagtaa ataatgcagg tattactcgc
    39301 gataatttat taatgcgtat gaaagaacaa gagtgggatg atgttattga cacaaactta
    39361 aaaggtgtat ttaactgtat ccaaaaagca acaccacaaa tgttaagaca acgtagtggt
    39421 gctatcatca atttatcaag tgttgttgga gcagtaggta atccgggaca agcaaactat
    39481 gttgcaacaa aagcaggtgt tattggttta actaaatctg cggcgcgtga attagcatct
    39541 cgtggtatca ctgtaaatgc agttgcacct ggttttattg tttctgatat gacagatgct
    39601 ttaagtgatg agcttaaaga acaaatgttg actcaaattc cgttagcacg ttttggtcaa
    39661 gacacagata ttgctaatac agtagcgttc ttagcatcag acaaagcaaa atatattaca
    39721 ggtcaaacaa tccatgtaaa tggtggaatg tacatgtaat atatttgagc taaagctcat
    39781 tgacgcagtg gttgactggt catccaatgg agaattgtct gacctagtca actttgcggg
    39841 ggaaattcta agcaacctag ataaggttcc agaatttctc cctaagaaac actaatcaat
    39901 aaattgataa gtgtttctaa aatttctact tgttttttag aatttaaaat gggaaaatat
    39961 agtagtctat gtataggcat ttttaaagga ggtgaatcga cgtggaaaat ttcgataaag
    40021 taaaagatat catcgttgac cgtttaggtg tagacgctga taaagtaact gaagatgcat
    40081 ctttcaaaga tgatttaggc gctgactcac ttgatatcgc tgaattagta atggaattag
    40141 aagacgagtt tggtactgaa attcctgatg aagaagctga aaaaatcaac actgttggtg
    40201 atgctgttaa atttattaac agtcttgaaa aataataaat cttacatctg ggtcgtcagt
    40261 attgtcgact cagttttttt ctttaattat caatagtttt aacgtaaaat taaagatgat
    40321 tcaagagcaa cacataaagg agataaaata atgtctaaac aaaagaaaag tgagatagtt
    40381 aatcgtttta gaaagcgctt tgatactaaa atgacagagt taggctttac ttatcaaaat
    40441 attgatttat accaacaagc attttcgcat tcgagtttta ttaatgattt taatatgaat
    40501 cgtttagacc ataatgagcg tttagagttt ttgggtgatg cggtattaga attgacggtt
    40561 tcacgatatt tatttgataa acatcccaac ttgccagaag ggaatttaac aaaaatgcgt
    40621 gccactattg tatgtgagcc ctcacttgta atatttgcga ataaaattgg attgaacgaa
    40681 atgattttac ttggtaaagg tgaagagaaa acagggggac gtacaagacc atcattaata
    40741 tcagatgcat tcgaagcatt tattggggca ttgtatttgg atcaaggact agatatagtt
    40801 tggaaatttg ctgagaaagt cattttccca catgtagaac aaaatgagtt attaggcgtg
    40861 gtagatttta aaacacaatt ccaagaatat gtgcaccagc aaaataaagg tgatgtaacc
    40921 tataatttaa taaaagaaga gggaccggca catcatcgtc tattcacttc agaagttatt
    40981 ctgcaagggg aagcaatagc tgaaggtaaa gggaaaacga aaaaagaatc agaacaacgt
    41041 gctgctgaaa gtgcctataa gcaattaaaa caaattaaat agaaatttat atgatacgac
    41101 atataggtgt tcgatttagg atttaaggaa gaatttttaa tattttatgg aacaacttcg
    41161 aaatttaata tacaaatact aaaatatcgt atacatgtgt tctttaaaat tgtgataagg
    41221 agtttaggat ggtttattta aaatcaatag atgccattgg atttaagtct tttgcagatc
    41281 aaaccaatgt tcaattcgat aaaggtgtaa ctgcaattgt tggtccaaat ggaagcggta
    41341 aaagtaatat tacagatgct attaaatggg tgttgggtga acaatcggct aaatcattac
    41401 gtggctcaaa aatggaagat attatcttct caggtgcaga acatcgcaaa gctcaaaatt
    41461 atgctgaagt acagttaaga ttagataatc attctaaaaa gctcagtgtt gatgaaaacg
    41521 aagttattgt aacaagaaga ttgtatcgaa gtggtgaaag tgagtactac ataaataatg
    41581 accgtgcaag attaaaagat attgccgatt tatttttaga ttctggattg ggaaaagaag
    41641 cgtatagcat tatctcgcaa ggtagagttg atgaaatact aaatgctaaa ccaattgata
    41701 gacgtcaaat tattgaagaa tcggctggtg tacttaaata taaaaaacgt aaggctgaat
    41761 cattaaataa acttgaccaa acagaagata atttaacgag agtagaagac attttatatg
    41821 atttggaagg tcgcgtagaa cctctaaaag aggaggcagc tatagctaaa gaatataaga
    41881 cactttcaca tcaaatgaaa catagtgaca ttgtagttac agtgcacgat attgatcaat
    41941 atacaaatga caatagacaa ttagatcaac gtttaaatga tttacaaggc caacaagcaa
    42001 ataaagaagc tgacaagcaa cgtttaagcc aacaaattca acaatataaa ggtaaacgtc
    42061 atcaacttga taatgatgtt gaatcgctta attatcaatt agtaaaagct acggaagcct
    42121 ttgaaaaata tacgggacaa ttaaatgttt tggaagaacg taagaaaaat caatctgaaa
    42181 caaatgcacg atatgaagaa gaacaagaaa atttaatgga gcttttagaa aatatatcaa
    42241 atgagatttc tgaagctcaa gatacttata agtctctgaa aagtaaacaa aaagaactca
    42301 atgctgtcat tcgtgaactt gaagaacaac tatatgtttc agacgaagca catgatgaaa
    42361 aattggaaga aattaaaaac gaatactata cattaatgtc agagcaatca gatgttaaca
    42421 atgatattcg ttttttaaag catactatag aagagaatga ggctaaaaaa tcaagactag
    42481 attctcgatt agttgaagtt tttgagcaat tgaaagatat tcagggtcaa ataaaaacga
    42541 caaaaaaaga atatcaacag accaacaaag aactttctgc tgtagataaa gaaattaaaa
    42601 atatagaaaa agacctcact gatacaaaaa aagcacaaaa tgaatacgaa gagaaattgt
    42661 atcaagcata tcgatatacc gaaaaaatga aaacacgtat tgatagtttg gcaacgcaag
    42721 aggaagaata tacttatttt ttcaatggcg tcaaacatat tttgaaagct aaaaataaag
    42781 aattaaaggg tatttatggt gcagttgcgg aaattattga tgtgccatct aaattaactc
    42841 aggcaattga aacagcatta ggtgcttcat tacaacatgt cattgtagat tcagaaaaag
    42901 atggacgcca ggctattcaa tttttaaaag aacgtaattt aggtcgtgcg acgtttttac
    42961 cattaaatgt tatacagagt agagtggtag cgactgatat taaatctatt gctaaagagg
    43021 caaacggatt tattagtatc gcttcggaag cagttaaagt agcaccagaa tatcaaaata
    43081 ttatcgggaa tttattaggt aatacgatta tcgttgatca tttaaagcat gcaaatgaat
    43141 tggcacgtgc gattaaatat cgaactcgta ttgttacttt ggaaggtgat attgtaaatc
    43201 ctggtggttc tatgactggt ggtggcgctc gtaagtcaaa aagtattctg tctcaaaaag
    43261 acgagttgac aacaatgaga caccaattag aagattactt gcgtcaaaca gaatcatttg
    43321 aacaacaatt taaagagttg aagataaaaa gtgatcaatt aagtgaactg tattttgaaa
    43381 aaagtcaaaa gcataataca cttaaagagc aagtgcatca ttttgaaatg gagctcgata
    43441 gattaactac acaagaaaca caaataaaaa atgatcatga agaattcgaa tttgaaaaaa
    43501 atgatggtta tacgagtgac aaaagtcgac aaactttgag tgaaaaagaa acttatctag
    43561 aaagtattaa agcatcttta aaacgactag aagatgaaat tgaacgctac acaaaacttt
    43621 ctaaagaagg taaggaaagc gttactaaaa cacaacaaac cttacatcag aaacaatctg
    43681 atcttgctgt ggttaaagag cgtattaaaa cacaacaaca gacaatagat cgattaaata
    43741 atcaaaatca acaaactaaa catcaattaa aagatgttaa agaaaaaatt gcattcttta
    43801 attcggatga agtgatgggc gaacaagctt ttcaaaatat taaagatcaa attaatggtc
    43861 aacaagaaac gagaacacgc ttatcagatg aattagataa attgaaacaa caacgtattg
    43921 agttgaatga acaaatcgat gcgcaagaag ctaaactaca agtttgtcac caagatattt
    43981 tagctatcga aaatcactac caagatatta aagctgaaca atcaaagcta gatgtattaa
    44041 ttcatcatgc gatagatcat ttaaatgatg aatatcaatt gactgttgaa cgtgcgaaat
    44101 ctgaatatac gagtgatgaa tcgattgacg cattacgtaa aaaagttaag ttaatgaaga
    44161 tgtcgattga tgaactaggt cctgtaaact taaatgcaat tgaacaattt gaagagttaa
    44221 atgaacgtta tacattttta agtgaacaac gtacagatct tcgtaaagct aaagaaacat
    44281 tagagcaaat tataagtgaa atggatcaag aggttactga aagatttaaa gaaactttcc
    44341 atgctattca aggacatttt acagctgtgt tcaaacaatt gtttggtgga ggcgatgcag
    44401 aattgcaatt aactgaagcc gattatttaa cagctggtat tgatattgtg gtacaaccac
    44461 cgggtaaaaa gttgcaacat ttatcgttac tgagtggtgg tgagcgtgca ttaactgcta
    44521 ttgctttact atttgcaatt ttaaaagtaa gatctgcacc ttttgttata ttagatgagg
    44581 ttgaagctgc actagatgaa gcaaatgtta ttagatacgc aaaatattta aatgagttat
    44641 cagacgaaac acaattcatt gttattacac accgtaaagg aacaatggaa tttgcagata
    44701 ggttatacgg tgtaacaatg caagaatcag gtgttactaa acttgtgagt gtgaatttaa
    44761 atacaataga tgatgtgttg aaggaggagc aataatgagc ttttttaaac gcttaaaaga
    44821 taagtttgca acaaataaag aaaatgaaga agttaaatcc ttaacagaag aacaaggtca
    44881 agacaaatta gaagatacac attctgaagg ttcaacgcag gacgcaaatg atttagcaga
    44941 aaatgctgaa gtgaaaaaga agccacgcaa gttgagtgaa gcggattttg atgacgatgg
    45001 cttaatatca attgaagatt ttgaagaaat tgaagctcaa aaaatgggtg ctaaatttaa
    45061 agcaggactc gaaaaatctc gtcaaaattt ccaagaacaa ttaaataatt tgatagcgag
    45121 atatcgtaaa gtagatgaag acttttttga agctttagaa gaaatgttaa tcactgcaga
    45181 cgtcggtttt aatacagtga tgacgttaac tgaagaatta cgtatggaag cacaacgacg
    45241 taatattcaa gatactgaag atttgcgtga agtcattgtt gaaaaaatcg tagagattta
    45301 ccatcaagaa gatgataatt cagaagctat gaacttagaa gatggtcgtt taaatgtcat
    45361 tttaatggtt ggtgtgaatg gtgttggtaa aacaacaaca attggaaaat tagcttaccg
    45421 atataaaatg gaaggtaaaa aagtaatgtt agctgcgggc gatactttta gagcgggtgc
    45481 tattgatcaa ttgaaagttt ggggcgaacg tgttggtgta gacgtaatta gccaaagtga
    45541 aggttctgat ccagctgctg ttatgtatga tgcgattaat gccgctaaaa acaaaggtgt
    45601 tgatatttta atctgtgata ccgctggacg tttacaaaat aaaacaaatc taatgcaaga
    45661 attagaaaaa gttaagcgtg taattaatcg agcagtgcca gatgcgcctc atgaagcatt
    45721 actatgttta gatgctacaa ctggtcagaa tgcgttgtca caagctagaa actttaaaga
    45781 agtaacaaat gttacaggta ttgtattaac gaaattagat ggtacagcca aaggtggtat
    45841 cgtattagcc attcgtaatg aattgcacat cccagttaaa tatgtaggtt taggtgagca
    45901 attagatgac ttacaaccat ttaaccctga aagttatgtc tacggcttat tcgctgatat
    45961 gattgaacaa aatgaagaaa taacaacagt tgaaaatgat caaattgtaa cagaagaaaa
    46021 ggacgataat catgggtcaa aatgatttag ttaaaacgtt acgaatgaat tatttgtttg
    46081 atttttatca atccttattg acgaataaac aacgtaatta tttggaatta ttttatcttg
    46141 aagattattc tttaagtgaa atcgcagata cttttaatgt gagtagacaa gcagtttatg
    46201 ataatataag aagaactggc gatttagttg aagattatga aaagaaattg gaattatacc
    46261 agaaatttga gcaacgccga gaaatatatg atgaaatgaa acaacattta agtaatccag
    46321 aacaaataca acgttatatt caacaattag aagacttaga atagatataa attgagggag
    46381 gacatcgata tggcatttga agggttatca gaacgcttgc aagcgacgat gcaaaaaatg
    46441 cgtggtaagg gtaaacttac tgaagctgat ataaagataa tgatgcgtga agtaagatta
    46501 gcgttacttg aggctgacgt aaactttaaa gtggtaaaag aatttattaa aacagtatca
    46561 gaacgcgcat taggttccga tgtaatgcaa tcattaacac cagggcaaca agttattaaa
    46621 atagttcaag atgaattaac gcagttgatg ggtggagaaa atacgtcgat taatatgtca
    46681 aataaaccac ctactgttgt tatgatggtt ggtttacaag gtgctggtaa aacaacaact
    46741 gcaggtaaat tagcattatt gatgcgtaaa aaatacaaca aaaaacctat gttagttgca
    46801 gcagatattt atcgtccagc agcgataaat caattacaaa cagtagggaa acaaattgat
    46861 attcctgtat acagtgaagg agatcaagta aagccacaac aaattgtaac taatgcatta
    46921 aaacatgcta aagaagaaca tttagacttt gtaatcattg atacagcagg tcgattacac
    46981 atcgatgaag cattgatgaa cgaattaaaa gaagtaaaag acattgctaa accaaacgaa
    47041 attatgttag ttgtcgattc aatgacgggt caagatgctg tcaatgttgc agaatctttt
    47101 gacgatcaac ttgatgtcac aggtgttacc ttaactaaat tagatggtga tacacgtggt
    47161 ggtgcagctt tatctattcg ttcggtgaca caaaaaccaa ttaaatttgt tggtatgagt
    47221 gaaaagttag atggtttaga gctattccat cctgaacgta tggcatcacg tattttaggt
    47281 atgggtgatg tgttaagttt aattgaaaaa gcgcaacaag atgtggatca agaaaaagca
    47341 aaagatttag agaaaaagat gcgtgagtca tcgtttactt tagatgattt tttagaacaa
    47401 cttgatcagg tgaaaaatct aggaccactg gatgatatta tgaaaatgat tccaggtatg
    47461 aataaaatga aagggctaga taagcttaat atgagtgaaa agcaaattga tcatattaaa
    47521 gcgattatcc agtcaatgac gccggctgaa agaaacaatc cagacacatt gaatgtatca
    47581 cgtaaaaagc gtattgctaa agggtctggt cgttcattac aagaagtcaa tcgtttgatg
    47641 aaacaattta acgatatgaa gaaaatgatg aaacagttca ctggtggcgg taaaggtaaa
    47701 aaaggtaaac gcaatcaaat gcaaaatatg ttaaaaggta tgaatttacc gttttaatgt
    47761 ttaatataac acgctgattt ttgttgcaat gagatgtgca tcccgaaagt tatactaaag
    47821 aatataactt tatggggtgc atatttttag atacacaata ataattaaaa actgaaattg
    47881 ctgatttgct agaacatgag gttattgttg ctaatattaa taagttatat tgggggaacg
    47941 tgtgcggtag aagtggtacc attcatgtcc ttacgagata aattaactta acacgtaggt
    48001 ggtgaaattt actatgaatt tttataacct attaacagtc taatttagta atcacatagt
    48061 tattattgta agaaaaaggt tcaataattt tcctggtaaa gaaaaaactc tttacaaaca
    48121 ttcatacacc tgttaatatt atttcttgta gaaaataaaa attaaaacat gacttaaagg
    48181 agattttata aatggcagtt aaaattcgtt taacacgttt aggttcaaaa agaaatccat
    48241 tctatcgtat cgtagtagca gatgctcgtt ctccacgtga cggacgtatc atcgaacaaa
    48301 tcggtactta taacccaacg agcgctaatg ctccagaaat taaagttgac gaagcgttag
    48361 ctttaaaatg gttaaatgat ggtgcgaaac caactgatac agttcacaat atcttatcaa
    48421 aagaaggtat tatgaaaaaa tttgacgaac aaaagaaagc taagtaattt agcgtaaaat
    48481 tgttctaaca ataagaataa ctcgtttaca ctgacagtta ttactcaatg atacgttggg
    48541 aatatcacat gttagtaata tagaacgtat tgggtaccat aatggtgccc tttttctttg
    48601 aattattttc aattaaaata gaagtgttca aagcatagag ttggaggtaa tagaatgaga
    48661 gttgaagttg gtcaaattgt taacacacat ggtattaaag gtgaaattaa agtaaaatcc
    48721 aattcagact ttacagacgt tcgttttcaa cccggtcaag tgctgacagt tgtgcataac
    48781 aataacgacc ttgaatatac tgttaagtca catagagtgc ataaagggct tcatatgctt
    48841 acatttgaag gtattaataa tattaatgat attgagcatt taaaagggag ttctatttat
    48901 caagagcgtg atcatgaaga tatcgtactt gaggaaaatg aattttatta ttcagatatt
    48961 ataggatgta cagtttttga tgatcaagaa acaccaatag gtcgtgtaat taatatattt
    49021 gaaacaggtg cgaatgatgt gtgggtgatt aaaggatcta aagaatattt gattccttat
    49081 attgctgatg ttgtaaaaga agtggatgtt gaaaataaaa aaattatcat cacgccaatg
    49141 gaaggattgt tggattaatg aaaattgatt atttaacttt atttcctgaa atgtttgatg
    49201 gtgttttaaa tcattcaatt atgaaacgtg cccaagaaaa caataaatta caaatcaata
    49261 cggttaattt tagagattat gcaattaaca agcacaacca agtagatgat tatccgtatg
    49321 gtggcggaca aggtatggtg ttaaagcctg aacctgtttt taatgcgatg gaagacttag
    49381 atgtcacaga acaaacacgc gttattttaa tgtgtccaca aggcgagcca ttttcacatc
    49441 agaaagctgt tgaattaagc aaggccgacc acatcgtttt catatgcgga cattatgaag
    49501 gttacgatga acgtatccga acacatcttg tcacagatga aatatcaatg ggtgactatg
    49561 ttttaactgg tggagaattg ccagcgatga ccatgactga tgctattgtt agactgattc
    49621 caggtgtttt aggtaatgaa cagtcacatc aagacgattc attttcagat gggttattag
    49681 agtttccgca atatacacgt ccgcgtgaat ttaagggtct aacagttcca gatgttttat
    49741 tgtctggaaa tcatgccaat attgatgcat ggagacatga gcaaaagttg atccgcacat
    49801 ataataaaag acctgactta attgaaaaat atccattaac taatgcagat aagcaaatat
    49861 tagaaagata taaaatagga ttgaaaaaag gttagtattg tgctaacata atctgagtgt
    49921 ggaaacacta aaatcactat gatccgctgc tatatatttg tcgaggcaag aacataggtt
    49981 gaagaggaga atttataatg acaaatcaca aattaatcga agcagtaact aaatcacaat
    50041 tacgtacaga cttaccaagt ttccgtcctg gtgatacttt acgtgtacac gtacgtatca
    50101 ttgagggtac tcgtgagcgt atccaagtat tcgaaggcgt tgtaattaaa cgtcgtggcg
    50161 gtggcgtttc tgaaacgttt acagttcgta aaatttcatc aggtgttggc gtggaacgta
    50221 cattcccatt acacacacca aaaattgaaa aaatcgaagt taaacgtcgt ggtaaagtac
    50281 gtcgtgctaa attatattac ttacgtagtt tacgtggtaa agctgctaga atccaagaaa
    50341 ttcgttaatc agcatttaaa caaagctatg tatgagtcaa attcgactca aacaataaag
    50401 ccatctaggt cacttttatg gagtgatata gatggctttt tttgctgttt agttaatgta
    50461 aagttgataa tgtaattgtt tattccttct ctattcataa cgtgtttgct caaaatggtt
    50521 atataaacaa aaatctacta agtgccattg aaaaggactt agtagattta ttaaatatat
    50581 gcgtagcaac attatttttg tcttgcccat ctcgtgaaaa taatactaca tataatgcca
    50641 aatatagtaa ttgttataag taaataatag tatggtgggg tatagctcaa ttgaatcttt
    50701 gttatatttt taggtgcttt aatgccggtc ataacaccat ttacttgttc aactttaaga
    50761 ctttgatcac ctgatgtcgc tttcatacct tgattatatg ctgttggcaa aacaatatac
    50821 ccagatgaat ttttattttt agtaatagta taaccatgct ttgtcttact aactttgaca
    50881 gcttctaatg aatttgaagc gtctttaagc gtggtataat cttcgccgta tatccctttt
    50941 aaatttactc gatacttacc tttaggcaat gataatctaa ttctatctgg agctttaatg
    51001 cgtatcgtta cgggtgttac aacgcgtcga tatttataag tgagtttatt tctttcttgt
    51061 gtatattcat tcactttaac atcatgagct ttatccggcg aaagtaattc taaatccatt
    51121 tcaaaataca aatctttaaa ttgattagaa actgattttg gcaactgtac agttagacca
    51181 ccattatttt gtttaacttg taataaatgt tttgtaggag attgccaggc tgcactattt
    51241 aatttaattg ttgaatctga tagtaaattt ttattggctt taaaatgtgt attaacatct
    51301 ttaatattgt tagaaacaat cccttgcaac attgcttgtt ctttatctaa tggagatttt
    51361 aattctttat tggaaaagac cttatttgta atatgtgcac ttggataatg gatggtattt
    51421 ttagaatgaa tccaacgaac tttattgtct ttgtgttcag acttaatttt aaatccatat
    51481 ggtaagttgt catcatgatt cactctaatt cgatcattaa cattccaaag tgatagtaaa
    51541 ttttgacgat tgccaagtaa tctataagtg ctgtttttat cgattggcat attaatttgg
    51601 agtgtcttgt cataatattt taaaatgtct ccattaaaaa tactagaata taatgaaatg
    51661 ccattataat gatatataaa tggtgaattt aatgcatagt ctgacatata atcaatgcga
    51721 ttaaatgagc ctgttgcatt ttgatttatc ttttttataa gctggtttac atagttacta
    51781 tggtaatcat gttgtttcaa cgttgataat gattgttgat aaggtttgat tgccatgttt
    51841 ttgttgttat ctaaaatgac gatttgttga atcataacga ttaatactaa tattgcaacg
    51901 gttaattttt tataacgcca taaactaaat tttaaaataa cggcaagcac tattagcagg
    51961 ataataccta ctataagtgc aagtgggtgt gtcggtgata gtaatacata aagtattgcg
    52021 atgatgcata cgggtattgt tctgattaaa taatatttca tatttaatgt tgataaatgt
    52081 tgaataaaca atccgcaaag agcacttgat gatagtgcta agatatacac ccaacgcctt
    52141 tctggaaatg aaaaaccatt aaaagcactg tcgaaatact gtgataatga acctataaat
    52201 aatatccatg ttactattgc gaaaagtcta taaaagtaaa aacgatacag tttgaatgac
    52261 aataatgcaa cgatagtaag aattgaaatc gtaatataaa atccatcgct aaagaaaaaa
    52321 taatgataat caagtggtgt caaaaacggt atatcaacat tgggattttg ctttctgtca
    52381 ttttccaaaa acgcagaaat gccagtgaat aaaccaaata cacttgataa cacactcaaa
    52441 actgtagcag atatgacgca aattaatttt tgtgttctag agacaatgtc atatttgtaa
    52501 gtgaaaatga gtcgatataa atagtagcaa cctataataa tagcttgata ataactgaaa
    52561 taaaaattgc taaatagtgt taaggctatc gcaacaatga aaataccgat tttgcgttgt
    52621 tgaaaatatc tttccaaacc aagaatcgat aatggcaata aatataataa atttccataa
    52681 aatgaccaag taaaattaaa gtatataacg acagttgaca tgccgtataa aatcgtagcg
    52741 atcatatttg ctgagcgttt aaagtgtaat attttaaata agtagaaggt cacgacaaat
    52801 gttatgatag ctcgtatcat agccataata agttggtttg tcggccaaaa atgtattgtc
    52861 gtcggattaa atataccaac cgtttctcct attttaatga atagaaaatt tagccacatt
    52921 aaaggtgaca gcgaataata atatgatagt cctttcatat aatcgccacc taatccaaac
    52981 gatgcatcat ataaactaga aaaactacgt agatgttcat acaaatacat ttgaaatggc
    53041 atcatttgac ggaatccatc tccagccccg ctaaaaacag taccattcac aatataatca
    53101 tagatatgag tagaaaataa aataagcgtt aatattacac taatgaaagt tataacaaag
    53161 aattgtttga cgtttgaatt tagccacttt tttaacacaa cattatcctc aactttcaaa
    53221 tttaaaatta agtttaactg aaactaaagt taatgaggtt cttgataggt aaagacgaag
    53281 atgactgtgg aacagatacc ttatcatagt tacttaaact ttggatcatt ttcagtttat
    53341 cattaaacaa atatattgaa taataaaaat gtcatactga taaagatgaa tgtcacttaa
    53401 taagtaactt agatttaaca aatgatgatt tttaattgta gaaaacttga aataatcact
    53461 tatacctaaa tctaaagcat tgttaagaag tgtgacaatg ttaaaataaa tatagttgaa
    53521 ttaatgaatt tgttctataa ttaacaggtt atagatttta ataatgagaa aagaattgac
    53581 gaaagtaagg tgaattgaat ggttattcaa tggtatccag gacatatggc gaaagccaaa
    53641 agagaagtaa gtgaacaatt aaaaaaagta gatgtagtgt ttgaactagt agatgcaaga
    53701 attccatata gttcaagaaa ccctatgata gatgaagtta ttaaccaaaa accacgtgtt
    53761 gttatattaa ataaaaaaga tatgtctaat ttaaatgaga tgtcaaaatg ggaacaattt
    53821 tttattgata aaggatacta tcctgtatca gtggatgcta agcacggtaa aaatttaaag
    53881 aaagtggaag ctgcagcaat taaggcgact gctgaaaaat ttgaacgcga aaaagcgaaa
    53941 ggacttaaac ctagagcgat aagagcaatg atcgttggaa ttccaaatgt tggtaaatcc
    54001 acattaataa ataaactggc aaagcgtagt attgcgcaga ctggtaataa accaggtgtg
    54061 accaaacaac aacaatggat taaagttggt aatgcattac aactattaga cacaccaggg
    54121 atactttggc ctaaatttga agatgaagaa gtcggtaaga agttgagttt aactggtgcg
    54181 ataaaagata gtattgtgca cttagatgaa gttgccatct atggattaaa ctttttaatt
    54241 caaaatgatt tagcgcgatt aaagtcacat tataatattg aagttcctga agatgcagaa
    54301 atcatagcgt ggtttgatgc gatagggaaa aaacgtggct taattcgacg tggtaatgaa
    54361 attgattacg aagcagtcat tgaactgatt atttatgata ttcgaaatgc taaaatagga
    54421 aattattgtt ttgatatttt taaagatatg actgaggaat tagcaaatga cgctaacaat
    54481 taaagaagtt acgcagttga ttaatgcggt taatacaata gaagaattag aaaatcatga
    54541 atgcttttta gatgagcgaa aaggtgttca aaatgccata gctaggcgca gaaaagcgtt
    54601 agaaaaagaa caagctttaa aagaaaagta tgttgaaatg acttactttg aaaatgaaat
    54661 attaaaagag catcctaatg ctattatttg tgggattgat gaagttggaa gaggaccttt
    54721 agcaggtcca gtcgttgcat gcgcaacaat tttaaattca aatcacaatt atttgggcct
    54781 tgatgactcg aaaaaagtac ctgttacgaa acgtctagaa ttaaatgaag cactaaaaaa
    54841 tgaagttact gcttttgcat atgggatcgc gacagctgaa gaaatagatg aatttaatat
    54901 ttataaagct actcaaatcg ccatgcagcg agctattgat ggattatcag tacaaccaac
    54961 gcatttattg atagacgcga tgacgcttga taatgcactg cctcaagtat ctttaatcaa
    55021 gggtgatgca agaagtgtgt ccattgcagc tgcaagtatc atggcaaagg tttttcgtga
    55081 tgattatatg acacagttat ctaaagatta tcctgaatat ggttttgaaa aaaacgcggg
    55141 ttacggtacc aaacaacatt tactagcaat cgatgatatt ggcattatga aagagcatag
    55201 aaaaagcttt gaacctataa aatcgttact gtaaatcgta aaattcaatt aaatgataaa
    55261 aaagtagcat ttaagtttac aatagcgctt acatttttta taattgtaag ggaacttaac
    55321 ctaagtaaca ggaggatgga agatgaatat ccacgagtat caaggtaaag aaatatttcg
    55381 ttcaatgggc gttgcagttc cagaaggacg agtagcattt actgctgaag aagcggtgga
    55441 gaaagcaaaa gaattaaatt ctgatgttta tgttgtaaaa gcacaaattc atgctggagg
    55501 tagaggtaaa gcaggcggag taaaaattgc taaatcttta tctgaggtag aaacatatgc
    55561 aaaagaatta ttagggaaaa ctttggtgac acatcaaact ggtccagaag gtaaagaaat
    55621 taagcgttta tatatcgaag aaggttgtgc tattcaaaaa gaatattacg ttggattcgt
    55681 tattgatcgt gcgactgacc aagtaacatt gatggcgtct gaagaagggg gcactgagat
    55741 tgaagaagtt gctgcgaaga ctcctgaaaa gatcttcaaa gaaactatcg atccagtaat
    55801 cggactttca ccattccaag caagacgaat tgcgtttaat attaatattc ctaaagaatc
    55861 tgttaacaaa gctgctaaat tcttattagc actttataat gtattcattg aaaaagattg
    55921 ttcaatcgta gaaatcaacc cattagttac aacagctgat ggtgatgtat tggcattaga
    55981 tgctaaaatt aattttgatg ataatgcatt attcagacat aaagatgttg tagaattacg
    56041 tgatttagaa gaagaagatc cgaaagagat tgaagcgtct aaacatgatt tatcatacat
    56101 tgcattagat ggtgacatcg gatgtatggt taatggtgca ggtttagcca tggcaacaat
    56161 ggatacgatt aatcatttcg gtggaaaccc agccaatttc ttagatgcag gcggaagcgc
    56221 tactagagaa aaagtaactg aagcatttaa aatcatttta ggtgatgaaa atgttaaagg
    56281 tatttttgta aacattttcg gtggcattat gaaatgtgat gttatcgcag aaggtatcgt
    56341 tgaagctgta aaagaagtag atttaacttt accactagtt gtacgcctag aaggtacaaa
    56401 tgttgagtta ggtaaaaaaa tcttaaaaga ctcaggatta gcaattgaac cagcagcaac
    56461 aatggctgaa ggtgcacaaa aaattgttaa actagtcaaa gaagcataag gaaaggatgg
    56521 gagcactaag atgagtgtat ttatagataa gaatactaaa gtaatggtac aaggtattac
    56581 agggtctact gcccttttcc atacaaaaca aatgcttgat tatggtacga aaatagtagc
    56641 aggtgtgacg cctggtaaag gtggtcaagt tgttgaaggc gttcctgttt tcaacactgt
    56701 tgaagaagct aaaaatgaaa ctggggcaac ggtttcagtc atttacgttc cagcaccatt
    56761 tgctgcagac tcaattttag aagcagctga tgcagactta gatatggtta tttgtatcac
    56821 tgaacatatt cctgtattag acatggttaa agttaaacgc tacttacaag gtagaaaaac
    56881 acgtttagtt ggtccgaact gtccaggtgt gattacagca gatgaatgta aaattggtat
    56941 tatgcctggc tatattcaca aaaaaggtca tgttggtgta gtatctcgtt caggtacatt
    57001 aacatatgaa gcagtgcacc aattgactga agaaggtatt ggtcaaacta cagctgttgg
    57061 tattggtgga gacccagtca acggaacaaa ctttattgat gttttaaaag cattcaatga
    57121 agatgacgaa acgaaagcag ttgttatgat tggtgaaatc ggtggtacgg ctgaagaaga
    57181 agcagctgaa tggattaaag cgaatatgac aaaaccagtt gtaggcttta tcggtggaca
    57241 aacagcacct cctggaaaac gtatgggaca tgctggtgca atcatttcag gtggtaaagg
    57301 tactgctgaa gagaaaatta aaacattaaa tagttgtggt gtgaaaacag cggcaacacc
    57361 ttcagaaatt ggttcaacat taattgaagc tgctaaagaa gcaggtattt atgaatcatt
    57421 attaactgtt aataaataaa gttaaaagat gatataaatg gctatagccc attccatact
    57481 ttataaaagt atcggaaatg ggctatttgt cgtgcgatga attttagtac acttttcata
    57541 ttttggaaag acaagttaat tattttacct cctacaatag ctttataaaa caaagaaaat
    57601 tttaattaaa atatatagcg cactatgtta taatgtttgt atattattgt aaggagtgtt
    57661 tcatcatgaa taaacaacaa agtaaagtac gctattcaat tagaaaagtt agtattggaa
    57721 ttttgtcaat ttcaataggt atgtttttgg cattgggtat gtcgaacaaa gcatatgcag
    57781 atgaaattga taaatctaaa gattttacaa gagggtatga gcaaaatgta ttcgcgaaat
    57841 cagagttaaa tgctaataaa aatacgacaa aagacaaaat aaaaaatgaa ggtgctgtta
    57901 aaacatcgga cacaagttta aagttagaca acaaatcagc aatttcaaac ggaaatgaaa
    57961 ttaatcaaga tataaagatt tcaaatactc cgaaaaactc aagccaaggt aacaatctag
    58021 ttattaataa caatgaactt actaaagaaa ttaaaattgc aaacttggaa gctcaaaatt
    58081 ctaatcagaa gaaaacgaat aaagttacta ataattactt tggttactac agttttagag
    58141 aagctccaaa aacacaaatc tatactgtaa aaaaaggaga cacacttagt gctatagcat
    58201 taaaatacaa aactacagtt tcaaatattc aaaatacaaa taatatagca aatcctaatt
    58261 taatatttat tggtcaaaaa ttaaaagtgc caatgacacc attagtagaa ccaaaaccaa
    58321 aaacagtgtc ttcaaataat aaaagtaata gtaatagcag tacattaaat tatttgaaaa
    58381 cattagagaa tagaggatgg gatttcgacg gtagttatgg atggcaatgt ttcgatttag
    58441 ttaatgtata ttggaatcat ctttatggtc atggattaaa aggatatgga gctaaagata
    58501 taccatatgc aaataatttt aatagtgaag ctaaaattta tcacaacaca ccaactttca
    58561 aagctgaacc tggggactta gtggttttta gtggaagatt tggtggagga tatggtcata
    58621 cagctattgt cttaaatggt gattatgatg gaaaattaat gaagttccaa agtttagatc
    58681 aaaactggaa taatggtgga tggcgtaaag cagaggttgc acataaagtt gttcataatt
    58741 atgaaaatga tatgattttt attagaccat ttaaaaaagc ataatttaaa tcgaaggcag
    58801 gacattgaaa tatgaaattt tcaactttaa gtgaagaaga atttaccaac tacaccaaaa
    58861 agcacttcaa acattataca cagtctatag aattatataa ttatagaaat aaaataaatc
    58921 atgaagcaca tattgtggga gtgaagaatg ataaaaatga agttatagct gcatgtttat
    58981 taacagaggc acgaattttt aaattctaca aatatttcta ctctcataga ggtcctttac
    59041 ttgattattt cgatgctaaa ttagtttgtt acttttttaa agaattatct aaattcattt
    59101 ataaaaatag aggagtattt attcttgttg atccatattt aatagagaat ttaagagatg
    59161 caaatggtag gataataaag aattataata attcagtgat agtaaagatg ctagggaaaa
    59221 ttgggtatct ccatcaaggt tatacaacag gatattcaaa taaaagtcaa attaggtgga
    59281 tttctgtatt ggatttaaaa gataaagatg agaatcaact tttaaaagaa atggaatacc
    59341 aaactagaag aaatataaaa aagactattg agattggtgt taaggttgaa gatttatcta
    59401 ttgaagaaac aaatcgattt tataaattgt ttcaaatggc tgaagaaaaa catggttttc
    59461 atttcatgaa tgaagattat tttaaacgaa tgcaagaaat atataaagat aaggcaatgt
    59521 taaagatagc ttgtataaat cttaatgaat atcaagataa attaaaaata caattattga
    59581 aaatcgaaaa tgaaatgatg actgtgaaca gagcattaaa tgaaaatcca aattctaaaa
    59641 aaaataaatc aaaatta
//
LOCUS       DALAAR010000008        51396 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000008 DALAAR010000000
VERSION     DALAAR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.008"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(128..565)
                     /locus_tag="O2458_000511"
     CDS             complement(128..565)
                     /locus_tag="O2458_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ3886400.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            complement(581..1705)
                     /locus_tag="O2458_000512"
     CDS             complement(581..1705)
                     /locus_tag="O2458_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCZ3886401.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            complement(1743..1994)
                     /locus_tag="O2458_000513"
     CDS             complement(1743..1994)
                     /locus_tag="O2458_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCZ3886402.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            complement(2006..2200)
                     /locus_tag="O2458_000514"
     CDS             complement(2006..2200)
                     /locus_tag="O2458_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCZ3886403.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2445..3149
                     /locus_tag="O2458_000515"
     CDS             2445..3149
                     /locus_tag="O2458_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCZ3886404.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            3690..4091
                     /locus_tag="O2458_000516"
     CDS             3690..4091
                     /locus_tag="O2458_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCZ3886405.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(4150..35415)
                     /gene="ebh"
                     /locus_tag="O2458_000517"
     CDS             complement(4150..35415)
                     /gene="ebh"
                     /locus_tag="O2458_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCZ3886406.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPRKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(35813..37204)
                     /gene="norB"
                     /locus_tag="O2458_000518"
     CDS             complement(35813..37204)
                     /gene="norB"
                     /locus_tag="O2458_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCZ3886407.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            complement(37361..38683)
                     /locus_tag="O2458_000519"
     CDS             complement(37361..38683)
                     /locus_tag="O2458_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3886408.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            complement(38714..39754)
                     /gene="tdcB"
                     /locus_tag="O2458_000520"
     CDS             complement(38714..39754)
                     /gene="tdcB"
                     /locus_tag="O2458_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCZ3886409.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            complement(39849..40967)
                     /gene="ald"
                     /locus_tag="O2458_000521"
     CDS             complement(39849..40967)
                     /gene="ald"
                     /locus_tag="O2458_000521"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ3886410.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            complement(41442..42320)
                     /locus_tag="O2458_000522"
     CDS             complement(41442..42320)
                     /locus_tag="O2458_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCZ3886411.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(42340..45780)
                     /locus_tag="O2458_000523"
     CDS             complement(42340..45780)
                     /locus_tag="O2458_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCZ3886412.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            46084..47424
                     /locus_tag="O2458_000524"
     CDS             46084..47424
                     /locus_tag="O2458_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ3886413.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(47630..47962)
                     /locus_tag="O2458_000525"
     CDS             complement(47630..47962)
                     /locus_tag="O2458_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ3886414.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(48046..49191)
                     /locus_tag="O2458_000526"
     CDS             complement(48046..49191)
                     /locus_tag="O2458_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCZ3886415.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(49400..49779)
                     /gene="rnpB"
                     /locus_tag="O2458_000527"
     ncRNA           complement(49400..49779)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O2458_000527"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(49842..50186)
                     /gene="gpsB"
                     /locus_tag="O2458_000528"
     CDS             complement(49842..50186)
                     /gene="gpsB"
                     /locus_tag="O2458_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCZ3886416.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(50200..50763)
                     /locus_tag="O2458_000529"
     CDS             complement(50200..50763)
                     /locus_tag="O2458_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCZ3886417.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(50756..51106)
                     /locus_tag="O2458_000530"
     CDS             complement(50756..51106)
                     /locus_tag="O2458_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCZ3886418.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            complement(51161..51340)
                     /locus_tag="O2458_000531"
     CDS             complement(51161..51340)
                     /locus_tag="O2458_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886419.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
BASE COUNT        13581 a         9196 c         8537 g        20082 t
ORIGIN      
        1 agaaaatata gttggtaaaa acggacttta tttaacaaat agaatacaac tatattctct
       61 attttcaatg acagacacca tttttaatat tataaaatgt gttaaccttt atatttattt
      121 atgtgtacta tttacaattt tcgtcaaagg catcctttaa gtccattgca atgtcattaa
      181 tatctctacc ttcgataaat tctctaggca taaaataaac taaatcttga cctttgaata
      241 aagcatacga aggactagat ggtgcttgct gaatgaattc tcgcattgta gcagttgctt
      301 ctttatcttg cccagcaaaa actgtaactg tatttgtagg tctatgttca ttttgtgttg
      361 caactgctac tgcagctggt cttgctaatc cagctgcaca gccgcatgta gagttaataa
      421 ctacaaaagt agtgtcatca gcatttactt ggttcatata ctccgatact gcttcgctcg
      481 tttctaaact tgtaaaacca ttttgagtta attcgccacg catttgttgc gcaatttctt
      541 tcatataagc atcatatgca ttcatattta attcctccaa ttaaattgtt ctgtttgcca
      601 tttgtttcca tactgaacca agtgcttcat ctccgttttc aatacgagat atggccattt
      661 caatttgtaa tttaacttca aacgcattgt cattaatatg ggcttttagt gcgggaagct
      721 gctctgcatt accttcatca aagataaaca tagcagcacg ccacctaacg attttctgtg
      781 gatcatctaa tagtagcacc atttctggta gtgcctctgg ataccctaaa tcgcttatgc
      841 aatcccctgc tgttcttctt acaacaggac ttttatcacg aagcccctta tataaatacg
      901 gtaaaatttc tttactttca atcataccta ataatacaat cgcttgacgt ctaaccggta
      961 ctttttcatc agataaagct aaatcaagca gcggtatatc ttcaaaagtc ggctttggaa
     1021 aatggtttaa cattcgtaat cgagtcttcc aattatcagt tgcatgatat tcatccaaag
     1081 agacatgtcg atagaaatga taattattag ttgcgtgatt ttcttctaaa gcatgtttta
     1141 ccaatacggg taactgtgat tctggatagg tagttagcac ttcttctagg acaccatcca
     1201 ttacttcttg aatatttcca tagcgatttc ctaaatctag ccatttacgc ataaaaacaa
     1261 tattgtcatg tgctgtttgc gcttgagtca tatggtcaac atatgtttgt ggtaattgtt
     1321 ctcttaattc ttggtcagca gaagttagct taatttgata cggtataccc ttaaaagtta
     1381 ataattcagc tttaatttca ccaaaatgat tgtcaatttg aggttcattt acagattcta
     1441 aaaccttatt cgcatcagaa aaagcagctt taatatcagg taatatgact tcccaatcag
     1501 cttttggtgc cttatcaaca gctaagaagt tcatgacatg aaaaatggaa gtgataccat
     1561 ctatagataa caactgattt ataaatcttg gttgtgtttc ttctactttt ttataagtat
     1621 tagataactt gtcttctctt gtatatgaca aaacaacttt cattgtattt ggacttggtg
     1681 ttggctctat acgtaaaatt tccatatact ttattccccc cgaatactag ttgatgaaaa
     1741 atttattcga atacagcctc tacttttggc aatactgttt cccaatttgc gtcattttct
     1801 ttatctactg aaataaagtc cataacatgg aaaattgatt taacgccttc aacctttaag
     1861 atgtcattaa taaatgctgg ctgtgaatca tcaactttag tatacgtatc tgatgtcata
     1921 ccttctctgc tttcactaag tgtaatcttc attgtgttgt ggttcggtgt ttctgatata
     1981 gatataattt tcatttgact tcctcctatt gaagttcatt tcctaattgt tttatttctt
     2041 tccctattga acactcattg atacaaaaat gatgcgcctt cgttttcccc tctaatttac
     2101 gcagacgagt tttgattgga cattgattgc aataagtatt cattaaatca tctattttcg
     2161 cgagtgcatt ttgctctgaa aaagttaaaa tacgcgacac ttcctttatt acttcaaaat
     2221 attgcaacct tgtaaataag tgttactgtt ttaaatttga cataaaaata ttataacatt
     2281 tttgacccta agatttgaat gaaacctttt acaattctat acgtaaaatg tatttatatt
     2341 caaatgctat tgcatcgcaa acgatttttc agtaaaatga gttagttatt tattgagcgg
     2401 tttctaaaca cccgcatgat caaaatttag gaggacattt taatatgtat aatgaaattt
     2461 taggactagt tacgtttatt gcaacgttcg tgcttatggt acttatgtat cgcttttttg
     2521 gtaaacaagg tttaattgca tgggttgcaa ttggcacaat cattgccaac atacaagtga
     2581 ttaaaactgt agaaatcttt ggtatttcag caactttagg taatgtcatg tttgcttcta
     2641 tttatttagc aacagatata ttaaacgata tttatgggcg tagagttgca aaaagagcag
     2701 tttggttagg cttttcatca acattaatta tgattattgt tatgcaattg tcattacatt
     2761 ttattcctgc accagaagat atggcacaaa aagcattaca cgcaatcttt gatgttgtgc
     2821 cacgcattgc tttaggctca atcgtcgcat atattattgg tcaacatatt gatgtattta
     2881 tcttttcact aattaaaaaa gtatttagtt ctgataaaac gtttttcatc cgtgcatatg
     2941 gtagtacatt tttaagctca ataattgata cagctttatt tgtagctatc gcatttatcg
     3001 gaagtttacc tggtacagtt gtatttgaaa tatttattac aacgtacgta ttaaaattag
     3061 cttcaacagt tttcaatgta ccatttggat atattgctaa atcattttat cgtaaaggta
     3121 agattgataa gttagatcaa ggctattaac gcacttacta cacaaaagaa taagttttaa
     3181 gggagtggga cagaaataat attttcgcaa aatttatttc gtcgtcccac cccaacttgc
     3241 attgtctgta gaaattggga atccaatttc tctttgttgg ggcccatccc aaacttgcac
     3301 attattgtaa gctgactttt cgtcatcttc tgtgttggga cccacacccc aactcgcatt
     3361 gcctgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga attgaaaaaa
     3421 gcttgttaca agcgcatttt cgttcagtca actactgcca atataacatc gtagagcata
     3481 gaacattgat ttatgtccca gcctgaagat gattttttta ggaagtatat tacagtacta
     3541 cgtattagaa aatcatcttt gaatcagcga gtattttaaa cacgtgttac ttatttaagt
     3601 gatgcgtgtt ttttaatgga ttacccaaca tattttgtat tacagtaata gtaactttaa
     3661 ctaagtattg aatagaaagt aggtttatta tggcgaaaat aaattttgat gctgcgacga
     3721 aaggaaatcc aggcataagt acatgtgcca ttgtaatcaa agaagatgag cagcattata
     3781 catatacaca tgagttaggc gaaatggata accacactgc agaatgggct gcatgtattt
     3841 atgcactaga acatgcacgt gaattaaatg ttcaaaacgc actattatat acagactcaa
     3901 agctaattgc agatagcatt gaagctggtt atgtgaaaaa cgcaaatttc aaaccttatt
     3961 ttgatcaaat agaaatattt gaaaaagatt ttgatttatt atttgttaaa tggataccga
     4021 gagaacaaaa caaagaagcg aatcaacacg ctcaacaggc attgtataaa ttaattaaaa
     4081 agaataaata aaaggagcac cgactgatgt gatgtcaatc ggtgctcctc tatttatcaa
     4141 agaaacaaat tattttttat ttttcttagc tttctttttc ttctttttag ctgctacctt
     4201 tttagaagta ttttttgatg ctgacttttt gtctttggat tggttatctt tttgattttt
     4261 ctttttagcc aataataaag gaacgtcctc atctttagat tcaatactag ttgttgtttc
     4321 aacatcttcc tctttatctt tgcgtcgttt tgcgaataag agtggtgaat gttcgttatc
     4381 tttcaacact ttttcatctg tgttttcatt tgtaacttcc acatcttctt catcttcttt
     4441 acgacgacgt tttggtaaga agaacggcgt atgtttaact ttatcgagtg actcgccatt
     4501 atttagcgaa tctttttctt caacagtaac atcttcttca tcttcttttc tgcgacgttt
     4561 cgcaaataaa agtggtaaat gttttgtatc gtctaaagtc tcttttattg aatctttatt
     4621 attatctctt atttctaatt cttcctcttc atcttcttta cgacgacgtt tggcaatgaa
     4681 gaaccagaaa ctagctagta aaccagagat acccaccaca ccaatagcgt ttttaataac
     4741 attaccgaaa ttattactaa agtgacgcat atgaagtgga tcaatgtcat catcttcatc
     4801 aagtttcttt ttatgtccaa tagtcgaact gttaaatgga tgatttgcag ttccataacc
     4861 aattgttaaa tggctattag attcatttcc agtacttgac gattgtttag cacgatcaga
     4921 tgttacaatt tgtactgctg aaattcgagc aataccattg ttcaatgcag cctcaacttg
     4981 ctgtaatgta tgcgcattat taatatctct aattgtttca agcacaattt cgttaatttg
     5041 attcattgct gcttgttttt catcagctgt gccaatttca ctatttcgaa tactatttat
     5101 tttttcattt gaaaagtctt taattctact aatagcttct tgcttgcgtt tagctagttc
     5161 ttttgctgtt ggattagctg ctttcatttg agctttaaat tgttccaatt gctcacttat
     5221 ttcatcgatg ctttgcgcac gttgaatcgc ttgaattgct tgttctttta attgatttaa
     5281 cttagcaata gcttcttgct tctctttatc tgttagatca gtacgagctt tgatttcatc
     5341 aatcatatgt tgaattgcat cttcaatcga tttaattgca tttgattttg cttgttcaat
     5401 cgtaaattgt tctggattaa attgttcaat atgcgcttgt tcttgttgtt gaatttcttc
     5461 aactgaatga acatcaggtg cattgttaac atgatcaatt gcttgttgtt taagcttatt
     5521 aacttctgca attgcagctt ctttttgttc cagtgttaat gtcacactat tattgatttc
     5581 attaatcttt tgttgtgccg cattttcgat tgattcaatt gcctgttgtt tgactactga
     5641 caattctttt tctacaactt ttacaggaac attaacaatc acttttgatc catcaagcaa
     5701 tgtaacttca acttttgctg ttaagctatc agaaatcgtt gcagttgatg gtgtatcgat
     5761 gacttctgct gaaagctgat caattaagtt tatgtgtgca agaatatctt gttctgtaat
     5821 gatgtcatca cgatgtacaa tgagttcacc attaactagg atttgctcag gtgttgcttc
     5881 aaaaatttca tttacagcag gttgttcatc aacctcccat acatgtgtat ttctaatgtc
     5941 atctaaacct aagtttaatc ctcgatttaa ttgatctata gtttgagcat tctcaacgtt
     6001 ttgtagtgct cgtttttcag cttcgtcaat ttctttgagc gcttttgctt tttgctcagg
     6061 tgttaaatct ggatttgaat tgatttgatc acgcttcgca ttagctaagg cttttattgc
     6121 atttttcgca tcttctttag ctttcactaa atctttgatg tcttgcaatg cttgtgcaag
     6181 ttgtgctttt gcctgctcaa tttcttcttt agtcagcgca ttgttaatgt cgttatgacc
     6241 ttgttgaagt atttgattaa ttcgatattt aagtgcttgt ttttccttat ctgttagatt
     6301 tggattttga tcgatttcgt caattaaagc ttgaacttgt ttatcaacat cttgtttcgc
     6361 atcttcttta gctttcacta aatctttgat gtcttgtaat gcttgcgcaa gttgtgcttt
     6421 ggcttgttca atttcttctt tagtcatcgc attgttaatg ccgttatgac cttgttgaag
     6481 tatttgatta atacgatctt taagtgcttg tttttcctta tctgttagat ttggatttcg
     6541 atcaatttcg tcaattaatg cttgaacttg cttatcaacg tctttattgg catcaatttt
     6601 tgcttttggt atttcattgg catgcacttg ttcaatcgcg tggttgcctg ctgtttgaac
     6661 ttgagataca gcctgattac ttgttgcttt attaatgttg ttgatgatgc tgtttgccaa
     6721 ttcttctgct ttatttttcg caataagctt gtcttgatcc gtcgcatttg aagcttcgat
     6781 ttcttttagc ttattagcta aagcttgatt aatagattga attgccttgt ctttagcatc
     6841 ttgtagtcgt tgatcaccat taagattatg gattgcatca ttgactgctt ggattgcgcc
     6901 attgatatca ttcacatttg tgttatcact atttagcaat gtatttgcta gacgtttggc
     6961 atcatcgaag tttgttttag cattatcgtc agcgttttgg taattgacag tttgctctgc
     7021 atttggaatt tcattgtcaa ctaaatgttt caatgtttcc attgcatcat ttaagtcaat
     7081 ttgattatta acaatatctg ttacatctga tacagtatcg gcattgttaa ttgctttatg
     7141 tgcaagatct tgttgctgtt gatttaatcc atttaacgaa ttaacaaacg catttgcttt
     7201 atctttggca tttgcaaggt tttggtctcc atttaatgca ttttgagcat cgataatatt
     7261 ttgtttcaat tgctctgctt cagcttttgc aattgcatta ccttgcactt tatctaactc
     7321 atgtgctgca ttcgcaattg cattatcata atttgctttc aaattgtcat ctgcattgat
     7381 gtaatttgtg ctgctgataa caccaaggtg ctgttcgtca atatattgtt gtactgcacc
     7441 cattgcatta tctaatttag ttgctctatt aattgctcga gatgctttat ttagcgtttc
     7501 agcattatta atttgttgga ttgctaattg tctttgagca ttgtttagat gtgttaattg
     7561 atccaatctt tgtaatgctt cagctttacg attattaagt ctttcttcac catttaatgc
     7621 tttcttagct gcagtgactg catcatttaa tttgataact tgttttgcag ttaagttttg
     7681 accagtcgat ttatcaagtg cttgtttcgc tttatcaacc gtttcatcat atacttgttt
     7741 gacttgtgta tctgcattga catagttcgt gctacctttc gtgcgtcctt cattgtcagt
     7801 gatttcttgt gacaattgat ccattgcacg attaagtgcc ttagcctcat ctacaatttg
     7861 ttggatttga tttaaatcgt ttgattgatc gatgcgacct ttaaatccat cttgttgagc
     7921 attgtttaat gcattcaatt ggtcgatgtc atgtttacca tttgttttag caagtgctac
     7981 acgttcatca ccatttaatg cttgttttgc atttaaaata ttttgaagtg cttgatccac
     8041 ttgttgctta ttcgcatttt gtttcaatac attttcagca tcagcaatag cttgtttata
     8101 agcattttgt ttatctgaat ctgcttgtgt gtaatctaca gtttgctcaa cgttagcatg
     8161 ttcattaact gcttgttgta attgatccat agattgattc aattgcgttg cttgatctac
     8221 taattcagca attggttgaa gtttcgtcgc ttgatcaatg ttttgtttag cagttgcaat
     8281 ttgatcagca tttaaatgtg ttaattggtc aatagtttgt ttcgcttgtg ttttagcttc
     8341 agcgactctc tcattaccat ttaactcatt ttctttttct tgaagcttag ttaatacttg
     8401 gtctacagcg tctttattcg catttgaacc attagttgga tctgtaatgc tttctgcagc
     8461 ttgtaacgct tgatctactg cttctttttt atcagttgac gcttcagtgt aatttggttg
     8521 agccttatct gtattcactt gatcaacttt atttttcaat gtttccatcg cgtgatcaag
     8581 ttcagtagca gtttgaacat gttgtgcaac ctctgttctt gtaggcgctg catttatagc
     8641 atcagttaat gcttgttgtt gtgcatgatt taagtttggc aatgcattta cagttgttac
     8701 tgcttgttgt tgatcacgag caagtttttg atcaccatgt agattatctt ttgcagttgt
     8761 tactgcttgt gtcaattgtt ctacttgtga tttgtcgagt gttggattac ctgtttggtt
     8821 aattaaatct tttgcatttt gaactgctgc ttggtaagca tctttttgcg gtttatcttc
     8881 attgataaac ttgctaccag attctgtttg ttgttgatct tgaatactat tacgtaatgc
     8941 ttgcattgct tgatcaagcg cttttgcttc agctaatttt tgtgctactt cgccacgagt
     9001 tgctgcgttg tttatttggc tttcaagtgc ttgacgttgc ggattattca aaccatttaa
     9061 ttgatttaaa tcagtaaccg catgttgttt atcgtctgca agtttttgat caccgtgtag
     9121 gttatcttta gcttggttaa cagcttgtgt caattgttca acttgtgctt tatcaagcgt
     9181 tggattgtta gtttgattaa ttaaatcttt tgcattttga actgctgctt ggtaagcatc
     9241 tttttgtggt ttatcttcat tgataaactt gctacccgct tccgtttgct gttgatcttg
     9301 aatgctatta cgtaaagctt ccattgcttg gttaagtgct tgtgcttcag ttaatttttg
     9361 tgctacttcg ccacgagttg ttgcattatt aatttgattt tctagcgctt gttgttgagc
     9421 tggtgttaat tgatctaaat gatttagaga atttccagca gtttgcttat cttgagctaa
     9481 tcgttcaaca ccatctaatg catttttaga tgatattact gcttgagtcg cacttgatac
     9541 attaccttta ttgatagttg gattatttaa tcctgcaatg atagactcag cattttgaac
     9601 tgcttcatca taggattgtt ttttattcgg ttctgcattg acatatgcac tgctcgcacg
     9661 tgttgcatct ttgtcagcaa tactttgttg taatgcatcc ataagttgat ctaatgcttg
     9721 tgcttcagtc aaatcttgct taactgctgt tctagttgtt tcactatcaa ttaacgtatc
     9781 ttccatatgt ttttgtgcat tgtttagatg tgctaattga ctaaccgttt gcttagcatg
     9841 atctttatca ttttgtaact tcacatcacc atgtaatgct gctttcgttg tattcacagt
     9901 tgtcgctgct tgattaatcg cattattatc taatgttgca gtttgttcat tgataatatt
     9961 atttgcggct tgaacagctt gatcatagtt ttgttgctct ggttgatctt cgttgatata
    10021 tttgctattt gctttagttg ctgcgttatc ttgaatgctc tgtcttaaac catgcattgc
    10081 ttggttaagc gtattcgcat tttgttcaat ttgatgaact gcagttacta aagttgcagc
    10141 tgtaacttgg tcttttaatg ctgttttttg aggatcattt aagcttgtta aagcatcaat
    10201 tgcttgcgtc gcatttgttt tagcaacttg taaattatgg tctccattta acgcttgaag
    10261 tgttgtttga actttagtaa tagcttgttc tacttctgtt tggttcgcat tttgaccagt
    10321 ttgtttgtta atcattgctg ctgcttcatt taccgctgta ttataagcag tttgtttatc
    10381 agtatcagca ttgatgaagt taccacgttg ttcaacggct tgatgatctg caatcgcatt
    10441 aattaagtta cccatcgcag tatttaaaga ttctgctgtt tgtttgtgac cattaactgc
    10501 agcaatatca gatgattgcg taatttgtga ttgtaactgt tgcttttgcg cattattcaa
    10561 atgatctaat tgatcaagtc tgttgtttgc ttgttgttta gctgctgcaa ggttttcatc
    10621 accatctaat gcaactttcg tattattcac ttgtgtcgtt gcaccattaa catcatctgg
    10681 attcatcgta ggcgatgtag tttgattaat gatattatta gcagctgtta cagcttggtt
    10741 atagtcattt tgtctttgcg cagttgcatc atgataatct tcagacgcta atgtcgtttg
    10801 attatccgca acactatttc ttaataattc catcgcatga tctaacgttt gtgctttttg
    10861 attaatcgta gtgacatctg taacaagtgg cgcttgtgta atttgacttt caaatgattg
    10921 tttttgtgca tcatttaagt gtgttaagtt cgccaattgt tgttttgctt gttgttttgc
    10981 atttgccaaa ttgtcattac catttaatgc ttgtttcgct gcgttcactt cattagtgat
    11041 gctttcaact tgtgcttgta cttcattagt accactttgt ttattcaata atgcttcagc
    11101 ttttgctact gcctgtttat acgcattttg tttgtcagga tctgcattta agtaattacc
    11161 gtccgctaat gtttgagctt tatcattaat agcttgtgat aattgatcca ttgcttgatc
    11221 taggctcgtt gctgtttgtt taacttggtt cactgtagca atttcaggcg attgatcgat
    11281 ttgacctttt aatgcttgtt tttgagcatc gtttaaatgc gtcatcccac ttacggcatc
    11341 tttagcattt tgcttttcag ttgccaattt agcttcaccg tttaagccat ttttagcatc
    11401 agtcacttga gttaatgcac gtgttatttc atctgggtta agcgttggat ttgtcgtttg
    11461 attgataata ccttccgctt gagacactgc atttgtatat gctgtttgct tatcggcatc
    11521 agcatcatga tagttctcac ttgctttgac agtatctttg tttgcaatac cttgtttcaa
    11581 gttactcatt gctgtattca cattttgtgc attttgttta gtttgttcaa ctgtagctaa
    11641 cgcttgtgct tgattgattt ggttacgtaa tgcatcacgt tgtggttgat ttaaatcgcg
    11701 taacgtatca agatttgcta aagcttcttg tttcgcttgt gctaattttt catcaccgtt
    11761 taatgcatct ttcgcttgat tcatagttgt cactgcttgt gttactgtat caggcgtcaa
    11821 tacaggtgtt ggtatgccat ttgcgatttg ttgcgcttgg ttagccgcat tgttatatgc
    11881 ttgttgtttg tcttgatccg cttgtgtaaa gtcaactgac tgtggtactt gactgttcgc
    11941 ttgaatttgt tgtttcaatg tacccattgc attatttaac gcatcagcat tttgcttaat
    12001 agcattaaca cctgtaacaa gttctgcatg cgacacttgg tcttttaatg ctgttttttg
    12061 tggttgattt aagtttggta actgatcaat ggctgtattt gcattgtctt tagcaacttg
    12121 taagttatgg ttaccgttta aatcaccctt agcttgattc acttgttgta acgcttgagc
    12181 cacttgttgt ggatccacgt ttgcatttgg tgtaccacta attatttgtt cagcatgtgc
    12241 taccgcattg ttgtaagcat tttgcttatc agtatcagca ttgacaaagt taccatttgc
    12301 tttagtttgg tctttatctg caataccttg tttcaattgt gtcattgctt gatttaaatc
    12361 ttgtgctgtt tgcttaatcg tgtttacatt tgcaacagtt tgcgcagcat cgacttgttg
    12421 ttttaatgca tctttttgcg cttgatttaa atcatttgca tttgtaatca cttgtttcgc
    12481 tgcatctttt gcacgttgta cattgtcatt accattcaat gcttgtttcg cttcgttcac
    12541 acgttgcatc gcttgttcca cttcagtttg acttgcattg ctgccatttg ctttagataa
    12601 aataccttct gcatgtgaaa ccgcattagt ataagcatct ttcttagctt gatctgcatc
    12661 agtaaagtta atgctattta atgtctcagt tttatcttgt aatgcattgt ttaagttagt
    12721 catagcatta ttcagatttt gagcattttg cttaacatta ttaactgttg caatatctgg
    12781 tgcttgttca acttgagttg ttagcgcttg tttttgagca tcatttaaat gatctagtgt
    12841 accaagtgtt gtcttagcat cttgtttggc atcagctaat ttttgattac cgttaagtgc
    12901 ttgatttgtg tcattcactt tagttaatgc ttgttgaatt gtatttggat ccattgttgg
    12961 atttgttgtt tgttttaaca attcttctgc ttttgttact gcattatcat atgctgtttg
    13021 cttgtcagta tcagcatcgt gatagttctc gctaccttta atttgtgctt tattcgcaat
    13081 accttgtttc aattgtgtca tcgcgtcatt aagcgttgtc gccgcttgtt gaatagcatt
    13141 gacgtttggt acaagtgttg cttgcgtgat ttgtttgctg tattcatcac gttgcgcttg
    13201 gtttaagttt ggcaattgat caatagcatg ttgaacattt tgtttcgccg ttgctaagtt
    13261 tgtattacca tttaaatcat ttttagcttg cgtaacttta tttaacgctg cagtaatttc
    13321 gctaggtgta acgacaacat caggcgtagc actaattaat gcttcagctt tcgctactgc
    13381 ttgattatat gcattttgct tatcaggatc tgcattgaca aagttaccat cagcttttgt
    13441 ttgttcttta tctgcaatgc cttgttttaa ttgtgtcata gcattgttta actcttgtgc
    13501 tgtttgttta acattgttta caccagctac agtagttgca ttttgaactt gttgttttaa
    13561 tgcgtctttt tgtgcttggt taaggtcatt agagctatta attaatgctg ttgcttcgtc
    13621 ttttgcatgt tgaacgttgg cattaccatt taatgcttgt tttgcagcat taacttgttt
    13681 gattgcttgt tcaacttcag cttgtgttgc attgccgcca ttagcttttg aaatgatatt
    13741 ttcagcattt gtaacagcag tgttatatgc ttgttgtttt gcttgatctg catcagtgaa
    13801 gttaactgtc gctttcgtag catctttgtc agctatcgct tgtttcaagt tacccatcgc
    13861 tgtatttaag ttttgcgcat tttgcttaat ttgatttgct tcatcaacag tatgcgcgcc
    13921 attaatttga tcagtaactg tttgtttttg cgcattattt aagttatcta atgaacctaa
    13981 tgattgtgac gcttgttgtt tcgctgcctc taggttttca ttaccattta atgcattttt
    14041 agttgtgctt acttgtccag cagcttgatt gataacagtc ggatctaaag ttggacttgt
    14101 agtttgatca ataatacctt gtgcagttgt gacagcatta ttgtacgcat cctttttatt
    14161 ctgacttgca tcagtataat tttggttttg ttttgttgtc gcattatctg caatactttg
    14221 acgtaatttg tccattgctg catcaacatt gtttgctttt tgctcattac cttgtgcttc
    14281 tgcaacagta gtcgattgtt gtaccaattg ttttaatgcc tctttttgtg catttgttaa
    14341 atggcttaaa ccgtcaattg ctgtatttgc gtgttgttta gctttttcaa ggttttgagt
    14401 accattaagc gctgcttttg tagcattcac ttgattcata gctgcttcaa cttgatcttt
    14461 aggcacgttc gtacctgtag atttatttaa aatattttca gcattatgaa ccgcttcatt
    14521 gtatgcattt ttcttctctg gatctgcatc tgcaaagttt tgactgccaa gtgtagtgtc
    14581 tttatcattc aagctatttt tcaagttacc catcgctgta ttcagttcct gagcggtttg
    14641 aatggcttgc gttacttcag ctacagtgtg cgcttgattg atgtttccag taacagcacc
    14701 tttttgtgca ttgtttaagt tatctaacgt agataatgtt tggtttgcat tgtttttagc
    14761 atctgttact tttctatcac cattcaacgc ttgtttcgta gttgttacag ttgaaagtgc
    14821 tgcttcaact tgtgattggt tcgcatttgt accatttgct tgattaataa tattttcagc
    14881 agcagttacc gcattgttgt atgctgtttg tttttctgga tctgcatctg taaagttaac
    14941 actttgtttc acttgatctt tgtcttggat tccgttgatt aaacgttcca ttgcttgatc
    15001 taattgttca gctttagttt tcacttgatt tactgtgcta atatttggtg catttgtcac
    15061 ttgcgtattt aaatcttgct tttgagcatt atttaaatga cttaattggt tgatgttagc
    15121 agtcgtttct tgctttttat tcgctaagtt ttgtgcacca ttaagcgcag ttttgttagt
    15181 gctcacttga ttagctgctt gtgttactgc agatggatca agtgtcggct gactcgttgc
    15241 attaatgata ttttcagcat ttgtaactgc tgtattgtat gcattttgtt tatctctatc
    15301 tgcatcaaca tatggttgac tcgcttttac atcatcttta ttcgcaatac tatttcgaag
    15361 ttgattcatt gcattatcaa gatttgtcgc gttgtctttc acactttgaa cgccagttac
    15421 ttgtgtcgcg ctatcaattt gaccagtaat actttgtttt tgtgcatcag ataaatgtgt
    15481 catttgacgt aatgcatctt tagcttgttg cttcgcagtt gctaagtttg tatcaccgtt
    15541 taaacctgtt tcagcattat taacacgttg tgttgctgct gtaacatctg ctgcatttgt
    15601 aatcacatta ggcgaaccat ttacaatgtt tttcgcagca ttatatgcat cagtataagc
    15661 accttgctta tcaggactcg cgttagtgta attaccacta gctacaattg tgtcatgatc
    15721 agcaattgct tgttttaatt gatccatcgc attattaagt gatgtagcac tatttttcag
    15781 atcagttaca cctgatacac gttgcgcagc gttcacttgt tgttttaatg ctgttttttg
    15841 tggtgtattc aaatgtgtca agttatcgat agcttgtata gcttcagttt tagcgcgttg
    15901 taagttcgcg tcaccgttca aagcattttc agtagacgtt acactttgaa ttgcttgttc
    15961 aacagcttgc ttatctttat tcgcaccgtg atctttattt aagtaagatt cagcttgtgt
    16021 gatagcatta tcaaatgctg ttttcttagt tggatctgcg tcgatgtaat tttcgctcgc
    16081 tttagtttga tctttatttg ctatagctga ttgtaatcgt tgcattgcac catctaagtc
    16141 ttgtgctttc gttttaacac catttacacc gttgactgtt gttgcgcctt cgatttgtga
    16201 tgttaaatct tgtttttgtg cattatttaa gtgtgttaat gtacctaagt ttgctttcgc
    16261 atctgatttt gcttgtctta catttgcgtc accgtttaaa gcattcttag cagtcgttac
    16321 ttgatttaat gcagcttcaa cttgtgcttt cgtcatattt tgaccgtgtg ctttatctaa
    16381 aataccttga gcattcgtaa cagcatttgt gtaagcatct ttcttacctt ggtcagcatc
    16441 tgtaaagttc acttgttgtt taacagtgtt ttggtcattt ataccatttt gaagttgacc
    16501 cattgctgta tttaattcat tagcagtatt ttgtgcagca gttacgcctg ctactgtcgt
    16561 tgcattgtca atttgactaa ttgctgcagt tgattgcgca ttattcaagt tactatatga
    16621 agctaatgca cgtttcgctg actcttttgc ttgagcaact ttattatcac cattcaagtc
    16681 atttttagct tgattcaatt ggtttagtgc tgcttgaact tgtgtttcat tcgcgttgct
    16741 gccattagca ttagtaattg cttctgcttg tgtaaccgca gtatcatacg cttgttgttt
    16801 tggttgatcc gcatctgtgt aattgacact acgtttcgta ttatcctttt cagcaagtgc
    16861 atgtttcaaa ttacccatcg ctgtgtttaa cgatgttgct ttatttttag ccgctgtgac
    16921 agctgcaaga tttggtgctt gatccacttc agcatttgcc gcagttcgtt gtgcattgtt
    16981 caagtctgtc aaacgaccga tatctgattt cgcagtttgt tttgctgctg ctaatttttc
    17041 atcaccgttc aatgcagact tcgcactgtt cacttggctc gctttttggt taattgtatc
    17101 aggattcatt tcagggttat tcgttgcact aataatacct tcagcattag ttaccgcatc
    17161 attgtatgca ttttgtaaat ctgcattcgc gtcttgataa tcttcgcttg atttagtcgc
    17221 atctttagaa gcaatacttt gtcttaattg attcattgct tgatctaaag taccggcatt
    17281 agcttgaata ccttgaacac ctgcaacagt cgtaccactt tcgatttgcg atgttaagtt
    17341 tgctttttga gcatcagtta agtgtgacat tgtcgctact tgttgtttag ctgtgttctt
    17401 cgcttcattt aatcgctcgt caccatttaa tgctgtttta gcagtattca cacgttgtaa
    17461 tgcttgttca acagctgctt tattgtcatt tgaaccagct gttttatcta ataacgtttt
    17521 agctgctgtt acagcgtcat tgtatgcagt ttgtttttct ctatctgcat cagtatattt
    17581 ctgagctgct tttgtagctg cttcatcatt aataccattt tgtaagttgc tcattgctgt
    17641 atttaattca gatgccgttg tagacacttg atttacacct gcaactgttg tcgcaccttc
    17701 aatttgtgat ttcaatgctt ctttttgtgc attattaatt gatgttaggt tattcaatgc
    17761 atttttcgca gttgtcttag cgttcgcaac attttgatta ccgttcaatt cgtttttagc
    17821 acgttgtaca ttttctaacg cagtttcgac accgtctttt gaagtatttg gaccttgtgc
    17881 tttatttaaa atttgttcag cttgcgtcac tgcatttgta tatgcattac gtttcgcttc
    17941 gtcggcatca gtgaagttaa caccttgctt aatcgtattc tgatcttgaa taccattttt
    18001 caagttcgtc atagctgtat ttaatgcatc cgcattattt tgtgcttgtg ttacttcatt
    18061 aacatgcgtt gcaccttcga tttgacgttt cactgcatct ttttgagcgt cagttaagtc
    18121 acttaagccg ttcaaatgtt gcttcgcatt tgtttgcgca gttcttaagt tttcttgacc
    18181 gtttaacgct tgttgtttag ctgtcacttg gtctttcgct tgattaattt cttgcgcatt
    18241 cattgatgga ctagttgttt gaccaatgat tgcttttgct gcagtgactg cactgttgta
    18301 gtcagtttgt ttgttttgac ttgcatctgt gtagttttga cctgctttaa tcgttgcttc
    18361 attcgcaatg ctatctcgta gacctttcat tgcagtgttt aatgtttgtg cattatcacg
    18421 aacagtttga acatttggca acgtcgtcgc ttgacccact tgttctttaa gttttgcttt
    18481 ttgcggacca tttaaagaag ttaaaccatc aattgcagtg ttagcatttt gtttcgctac
    18541 ttctaaatta tgattaccgt ttaactgcgt cttagcattc gttacttgcg ttgctgcatt
    18601 tgtaacatct gctggtgtta acgttggtgt tggtgtacca ctaacgatat tttcggcagc
    18661 tgtaactttt tcatcatatg cttgacgttt attcgcatca gcattgacat agttaccact
    18721 agcttttgtc tcagctttat cagcaatggc acgctttaaa gctgtcatcg cagtatttaa
    18781 ttcagtcgca gtatgttcaa caccatttgc tgcagataca cgtcctgcac ttgttacttg
    18841 tgcttttaat gcttcttttt gttttgtatt taagtccgaa gcatttgtaa tcgctgtgtt
    18901 agcagcctgt ttcgcacgat ctaagttttg aataccgttt aatgcagtat ttgcttgtgt
    18961 aacagcttgc attgctcttt caacatctgc tttaggtgta ttaccgccag ctgttttatt
    19021 taaaatagtt gctgctgcat ttactgcttg agaataagca gttcgtttag catcatcagc
    19081 atcttgataa ttttgacttt gtaacgtcgt gtctttatca cgaattgatg tttctaattg
    19141 acccatagca ccatctaatt gttgcgcttt ggctttaact gtattaacac cttcaacatt
    19201 tgttgcttgt gtaatttcat tgtctaacgc tgtacgttgt gcattattaa tgtgtgttaa
    19261 tgtacctaac gtttgtttcg cagctgcttt agcttcattt aatttcgcat caccgttcaa
    19321 cgccgtcttc gtactgttca cattttgcaa tgcttgttca actgctgctt tgtctacatt
    19381 ttgaccacta gcttttgtta aaattgcttt cgctgcattt actgcttgat cataagctga
    19441 tttcttactt ggctctgcat ctaggtattt ctgagtttgt tttgtttgtg tctcatcatt
    19501 gataccattt tgtaaactat gcattgcgtt atttaattct gttgcttttg cagtttcttg
    19561 atttacacca gctactgttg ttgcaccatc aatgctacgc gttaacgcat ctttttgtgc
    19621 attgttaatt gatgttaagt tattcaagtt gtttttcgca gttgtcttag cttgagctaa
    19681 gtttcgcgca ccatttaatg cctgttcttt agttgtcact tgtgatgtta cttgcgtaat
    19741 agtatttggt tccatcgttg ggttcgatgt ttgattaatg attgcttttg ctgcagtaac
    19801 tgcactgtcg tactcgttac gattatttgg acttgcgtca gtgtagtttt gacctgcttt
    19861 aattgttgct tcattcgcaa tactatctct taagcctttc atcgctgtat tcaacgtttg
    19921 agaactattt ttaacagttt gaacaccatc taatgtagtt gcactttgaa cttgttcttt
    19981 taattttgct ttttgtgcat tattcaattg tgctaagccg tcaattgtat tgttggcatg
    20041 ctctttcgct acacgtaagt tgttatcacc atttaatgct gttttagccg cattgacttg
    20101 tgatgccgca ctatttacgt cattgactgt taatgtcgca ttcggtgtac cactaatgat
    20161 attttcagcg ttagtcactt ttgaatcata cgcttgacgt ttttcttgat cagcattgac
    20221 ataattaccg cttgcttttg ttctttcttt atcagcaata gctgctttaa gtgctgtcat
    20281 cgcactgttc aattcagttg cagtgtgttg aacgttattt gcatcagata cacgttgtgc
    20341 attgttaact tgtgttttta atgcatcttt ttgtttttgg tttaagtcat gtgcgttgtt
    20401 aattgtgttc gtcgcatttg ttttagcttg agctaagttt tgtgcaccat ttaacgctgc
    20461 ttttgttgta ttgacttgat ttgccatgcc attaatcgca ttagcatcca tatttggatt
    20521 gttcgttgca ttaataacac catttgcatt atttacagca gtattgtaat tatttttgtt
    20581 gctgtcagat gcatcaagat aattttgact tgttttcgtc gtattatcat tttggatact
    20641 tgtacgtaat gcacccatgg cagtatttaa cgtattacct ttatctttaa caccatttac
    20701 acctgctaca ttttgcgctt gttcaacttg atgcttcaag ttgtcttttt gtaattgtgt
    20761 taagttaggt aaaccatcaa tcgtatttgt tgctgaagtt ttcgcatttc ttaagttgtc
    20821 agcaccattt aaagccgctt tcgctcttgt aactgcattt aatgcattat caacgtctgc
    20881 tttagatgtg ttaccaccag tttgtttatt taaaatgcct tcagccgctg tgacagcttg
    20941 cgtatatgca tttcgttttg attcatccgc atcaagataa ttttgatttc ttaatgtcgc
    21001 atctttatca ttgattgaac cttgtaagct attcattgcg ccgtctaatg tattggcatt
    21061 tgtttttaca gtattaacgc catcaacagt cgtcgcttga ttgatttggc cttctaagtc
    21121 agtacgttgt gcattcgtaa tatggtttaa agtgcctaag ttttgtttag ctgccgcttt
    21181 cgcttctgcc agttttgcat caccattcaa tgcatctttc gtacttgtta cttgttgtaa
    21241 tgcacggtca actgctgctt tatctgaatt tgaacctgtt tgtttattta aaattgcttt
    21301 cgcagcagct acagcttgat cataagcagt tttcttactt tgttcagcgt cacgatattt
    21361 ttgagtttgt tttgttgcat tttcatcatc aataccatgt tgtaattgac ccatagctgt
    21421 attaagttca tttgcagttt gttggatact tgttacattt gcaacacgtt gcgcacttgt
    21481 aacttgtgct tttaacgcat ctttttgcgc tttatttaag ttagtagcac catcgatggc
    21541 atttgttgct gtttgtttcg cttgcgttaa attatgtgta ccatctaatg catttttcgt
    21601 tgatgtcact tgtgtagcga tttggttaat tgcattagca tccatatttg gattgcttgt
    21661 tgcattaatg acaccattag cactatcaac agcattgtta tagtttgttt tgttacgttc
    21721 tgtagcatca agatagtttt ggccattttt cgtagctgtg ttatcttgta tgctatttct
    21781 taacgtaccc atagcaccat ttaatgtgtt ggcacttgat ttaacagtat ctacacctgc
    21841 aacattttgt gcttgttgca cttgttgttt caatttatct ttttgagcag ttgttaaatg
    21901 ttgaagtccg tttatagtag tgtttgcatt tgatttagct tgctctaaat tatggttacc
    21961 atttagggca cctttcgcat tagcaacttg tgacaacgcg ttttcaacgg cagttttgtc
    22021 taaatttgat ccactatttt tatttaaaat attttctgcg ttcgtaacgg cttgagtata
    22081 agcagttttc ttatctgaat cagcatcatg atagttttca cttgctagtg tatttgtttt
    22141 atcattaata ccattttgca agttagccat cgctgtattc aattgtgtac tcgtattaga
    22201 tacagcttca acaccagcta cagttgttgc ttgatcaatt ttagttgtta agtcacgttt
    22261 ttgtgcatta ttaattgacg ttaagttatt taatgcattt ttagctgcat tcttcgcatt
    22321 tgtaacgttt tgatcaccat tcaatgcatt tttagcactt gaaacatttt gaatagccgc
    22381 ttcaacatct tgtttagacg tatttgcacc ttgcgtttta ttcagaattg cttcagctct
    22441 gcttaccgca tttgtatacg catcacgttt agctttatct gcatcagtaa atttaacact
    22501 ttgtttaact gtgttttgat catggatagc ttgttccaag ttacccattt gcgtattcaa
    22561 ctcagttgct gcatttttag tagcagttac ctcactcaca tgacctgcac gatcaatttg
    22621 tgatgagatg gcagattttt ggttatttgt taagtgtgat aatgtattta agttttgctt
    22681 agcagtgttt tgtgcatttc ttaagttttc agcaccgttt aaaccatttt tagcattatt
    22741 cacttgtgtt gtagcttggg taatcgcact agtatccata gtcggattgt tagtttgatt
    22801 aatgatacct ttcgcatttg acacagcgct attatatgtt gattggttat tcggacttgc
    22861 gtctgtatag ttttgacttg ttttgactgt tgtttcgtta gcaatactat ctcttaagcc
    22921 tttcattgca ttgttcaatg cttgtccatt tgtttgaaca gtttgtacgt cttctaatct
    22981 attggcttgt cccacttgtt cttttaattt agcttttcga ggtgtattta attgtgttaa
    23041 tgcatcaata gcagtattgg cgttttgttt tgcttcacgt aatctttcgt caccatttaa
    23101 ttcttgtttc gcgctgttta cttgattagc tgcagctgtt acttctgaag gtgtcgtaac
    23161 aaccgttggc gtaccgctaa taatatgttc agcattggta actttagttg tgtaagcatt
    23221 ttgtttagtg ctatcggcat taacatattt actgcttgct aacgtattcg tcttatctgc
    23281 gatggcatgt tttaatgtgc ccattgccgt gttcagttca gtcgcattgt gctgtacatc
    23341 ttgtgcatta gatacgcgtt gagcaccatt agcttgtgct tttaatgcat ctttttgttt
    23401 ttgatttaaa tcagatgcgc cattgattgc tgtaatcgct gcttgtttcg cattgtttaa
    23461 gttttgcgta ccatttaatg catgtttcgc attattaaca ttattaagtg cttgttcgac
    23521 tgctgttttc gctgtattcg gtccagtttg tttatttaaa atggtttcgg ctgctgatac
    23581 agcttgattg tatgcattac gtttttgctc atcagcatcc aagaagtttt ggctcgctaa
    23641 tgttcctgac ttatcgttga tagccgtttg taagttaccc atagcaccat ctaaactatt
    23701 cgcattttgt ttaacagatt caacaccagc taagtttgta gcttgtgaaa tttgatttgt
    23761 taaatcatta cgttgagctg tagtgatatg cgttaaagta cctaaatgtt gtttcgctgc
    23821 gttttgagct gcaattaatt ttgcatcacc attcaatgca tctttcgcat tattaacacg
    23881 ttgtaatgct gcttcaactg cattttgcgc agtgtttgga cctgtcgatt tatttaaaat
    23941 cgctttcgct gcagtaatag cattatcata ctcttgttgt ttatttgtat cagcatcacg
    24001 atatttctca gatgatttca cttgagattc gttgttaata ccattctgta agctagccat
    24061 agcttggtct agatgttgcg cattttgttt tacagtatca acaccactca ctcttgtcgc
    24121 actagtaatt tgactaatca aattgttctt ttgagcatct gtaatacttg tcaatgtgtt
    24181 taagtacgtt ttcgcatttt gttttgcagc agctaagttt tcatcaccgt taagtgccgt
    24241 tttagaacta ttcacttgct ctgctttttg tgtaatcgta cttggattca tttctggatt
    24301 actattagca ttaataatcg tttcagcagc agctactgcg ttgttatatg ctgtttgctt
    24361 atcattatta gcatctacgt aatcttcact tgctttagtc gcatctttgt tggcaatact
    24421 ttgtcttaac gtattcatgg cttgatctaa tgtattggca tttgattgaa ccgtttgaac
    24481 accagcgaca gtagtaccac tattaatttg gtttgttaaa ttcgtttttt gtgcagttgt
    24541 taaatgcgtc atattattta actgctgttt tgctgtttgc ttcgcttgat ctaataaacg
    24601 cgtaccatct aagttatttt tagcagaatt cacttgattc atcgcttcag taacttgatc
    24661 tttcgtttta ttttgaccat ttgatttatt taaaatatct ttcgcagctt gtaccgcatt
    24721 tgtgtatgct gttttcttac taggtgtcgc atcttgatag ttttgactat taagcgtcgt
    24781 ttgttcatca ttgattgcac cttgcaagtt acccatcgct gtatttaaat ctgtaccttg
    24841 ttgtttaaca gtatttacgc cagcaatatt tgatgcagca ttaattttag atttaacatc
    24901 tgctttttgt gcattattta aatgtggtaa tgcatcaatt gctcttgcag catctgtttt
    24961 agattgtgct aatttttcat aaccatttaa tgcattttta ttagaagtaa cagctgaagt
    25021 tgcacgatta acatcatcaa cagacatcgt tggatttgtt gtttgattaa tgattgtttc
    25081 ggcacttgaa actgcactgt tatatgcatt ttgtttagct gtatctgcat ccgcataatc
    25141 ttctgtacgt ttcacttgat ctttatctgc aacagcttgt cttaagttac ccattgcact
    25201 attcaagttt gttgcatttt gcttaattgt attaactgca tcaatttgat gcgcaccatt
    25261 aatttgcgat tgtaagtttt gacgttgtgc attatttaaa ttgtttaaat gacctaatgc
    25321 agtattcgct tcttgtttcg cagcatttaa cttagcttca ccattcaatg catgttcttt
    25381 acttgtgaca tttgataaag cattgttaac atcacttggt gttataactg gatgttgtgc
    25441 attaccatta ataatgtcat tcgcatggtt gtatgcattg ttgtaatcat ttttcttatt
    25501 agtatctgcg ttgacataat tatcactttg tacgacttgg ttatgattag caacgccacg
    25561 ttttaaacct gtcatagcag tatttaagct ttgagtcgtt tgtttaatat catttactgc
    25621 ttgcacgttt tgtgcactat tcacttgttg tgttaatgca ttcttttgtg cttggtttaa
    25681 atcacttgca tgcgtaatcg catttgttgc ttctgttttc gcacgttgta agttttgttg
    25741 accatctaaa gcatctttcg tcgatttaac agatgctgct ttttggttca ctgtgttaac
    25801 atccatcgtt ggattagtcg tttctccaat gacaccttta gccgcattaa ctgcattatc
    25861 atacgcttgt ttattagctg gtgttgcgtc agtgtaattt tgatctgatt taattgtatt
    25921 attatccgca acactgtctt ttaatttcgt catcgcagta tttaaagccg taatgttaga
    25981 cttaattgtt tcaagcgcag catgattttg agcagcatta atttgttgcg tgaaattatt
    26041 ttgttgtgct tggtttaagt tagatgcacc atgtaatgtt gttaacgctg gctgtctatc
    26101 taatgttaaa ccttgacgtg ctgtatttaa ttgatcctta gcttggtttg cctctgtcac
    26161 tttatcattg atattttgga cagttgggtt gccattcaac acttgattaa tttcagttaa
    26221 cttttgacgc gctgcttgta atttttggtt gtacgcattt aacgaagcgg tcgtcatacc
    26281 tgttgtatcc gttggttgat taatgctttg ttctaattgc gttttcgcag tttgaagcgg
    26341 cgctttatct ggtgttaaag cttgacgtgc atgatctaaa tcagatttag cttgatttgc
    26401 tgtagacgta tttgtattaa tttgttctac agtcggtgaa cctgctaata cttgattgat
    26461 ttgttgaatc ttattacgtg cagctgttaa cttcgcattg tatgcattta tagagtcttg
    26521 tgtcatacca gttgtacttg tttgcgtgtc aatactatgt tgtagttgat ttttcgcatt
    26581 ttctaaaggc gctttatcga ctgttaagcc attacgtgct tgatcaagtg ctgatttagc
    26641 ggcattcgct gctgtcacgt tttgacgtat tgtcgcaaca tctggatgtg aggctaatac
    26701 acgatcaatt tcttgaattt tagttctagc tgctgaaagt ttttcattaa atgctgcaat
    26761 agatgcgctt gtcataccag tcgtactcgt tggctgatca atatcatttt gcaactgagt
    26821 ttttgcagtt tgtaatggtg ccaagtctgg agttaatcca gcaattgctt gttttaagct
    26881 attatatttt tcttctactt ttgttttttc tgcggcaatt tgttggtcag tcgcatcacc
    26941 attgttaata acattttgtg catttgttga ttctgtttga cccgcacgtt tagcattttc
    27001 atatgcttgg attgatgatt gtgtcatacc atcagtagtt actgatttat tgatttcttc
    27061 atcaagtttc gtcttagcag tttttaaagc actattatca gctaaaggta ctaattgatt
    27121 aattgcttgc gttaatcgct cattgacacg atttacattt gttaacgcag attgcacttc
    27181 ttgtactgtt cttattggct tttgaataat agcattagcg ctatttttag cacttgttaa
    27241 gtcactttga agtgcacgaa tcgaattatt gtaagcagta atacttgccg gcttcttacc
    27301 agtcgttgta cctgtgcgat tcaattgttg cactgcttgc tctaaggcat gtgtatctgc
    27361 agttaaatca tgtttcgctt ggtttaatgc tgttaatgcg ttatcgacac gatgtttttc
    27421 atctgaaatt tgttgtgcag ttgcatcgcc attgtcaata acacgttgag ctgcagttat
    27481 ttcagtttct gcttcacgct tcttcgcatt atagttatca atactttgtt gcgtcatacc
    27541 agcagttgat ggtacttggt tcacagaact ttgtaagtta tttttagacg ttactaattg
    27601 gctattatct tctttatttt gaagtaatgc tttagcttga tcaatcttag tttgtgctgc
    27661 acgaacttta gttagtgcgt cagaaacttg ttgtggtgtt gcacgctcat tattaatcac
    27721 ttgttgtgct tctgttttcg cagtattgat ttgttgttgc gcattatgca ttgcattatt
    27781 gtactgcgta attgtacctg gctttttacc ttcagtgctt actggatcat ctaaatgatt
    27841 tttagctgtg attaactcac gtttatccgc ttttgtgaaa atggactctt gtacttcttc
    27901 agtactacca tcattgtaag ttactgtcac aggaatcgtc gttgtgctac caccagctaa
    27961 attagtaggc attgctgtgc catttttaat cgttgcagta cgtttattag caacttgaac
    28021 tgcattgtta atttcagctg ctgttacatt tgaaccataa tctttcacaa tttcagttgt
    28081 gttgacagta tgagctgccg gtgcagttaa tgtacttgga ttactactta ctgagtgacc
    28141 tgtacctgct ttcggagtaa ttgtgattga tgaatttggt tttatagtat tggcattgaa
    28201 cgtcacttta ccagtttgtg catctaacgt tacatagtca ggcttattcg caattgtcca
    28261 ttgattattt tgaccacgaa caacattaat tgtcttacta tgttctgcac cattacccac
    28321 tttttcagtg taagcaatat ccattgcttg agttggatta attaaatgtc ctgatggatt
    28381 attaggcgtg atatcaatat gaccattttg ccaaatctta gtagtcgctt ggttcggttg
    28441 tggtgcgaca actgtgaaat catcactacg ttgctcatca ctcactgtct ctccgcttcc
    28501 ttgcgttgca acaacttgaa ttgtatcagc agggttgaaa gtacctgctg caacagtaat
    28561 accattatta gttccagcaa tacctgctac agttgctgca gatgcttctt tcacccatgg
    28621 actcgtatta ttgcgacgtg taaatgtcgt cacaacgtta ccattacgtt taataactaa
    28681 tttatcagcg tatgtcgtta cgttaccggc atgtgtattc actgtttggt ttgctccagg
    28741 tgcaattgta atcgctcctg ccgctgtttc agtcacagtt ggtttcgctg gttgcacatc
    28801 ttttactaca aatttcgctg gtaaagatgt tgcaaaagta tgtccgttat agatgacgtc
    28861 atattttgcg ttaacgactt tagccacatt cggtttattc atagctgacc agtttgcgtc
    28921 atttgtacct gtagtatcac gattccattt atacgtaaat ccatctgttg gtaaaccagt
    28981 agcattttgc atatgtgcat atcctgatgc ttgcgtacca cttgctaaag tgcctccaac
    29041 cgttgtcgta taagtagttt gagggaattc aaattgatat acattaacag taacaggaac
    29101 tcgtttagca gctgaaatac ctggatatgt gacatcgaca tttaaatgtt gcacgcctgc
    29161 ttgttggtta tttggttgtt gtctatttgc ccatgctgca gtgataccat ttgtgtttgt
    29221 atttggatca aatgtaatgt agttcatcgc atccgttcca ttagtcaaat tttgaccttt
    29281 cacatcacgt gatggcgcct ttgcattagc aactggataa actttgactg gaacttcaac
    29341 attacgcgta ccttgaccat taggtaatgt tacaaccgca gttttatgag tgttaccgac
    29401 tgtattcttc catgtatctg gactatcatg ccatgcaacc gttgccccat gtggcgggtt
    29461 ttgaataaat ctttctacgt gtccgaaatc aaaaccgtcg ccacctttaa taaatacagc
    29521 gccttcagta gttgcttgta attgtggtgt cactgttact gttgcactgt catttgaatc
    29581 aacagattca tttcttgtta ctgtaacaac ttgaccatgt tcgtcttgcg tcgtatacgt
    29641 cacattgttc attgaaattg aagattttgc tttaattccg ccatttggta acgcgtcact
    29701 tactgtcaca accgaactaa aaccgctacc atgagtaata tttgtgacat ttaatggtgt
    29761 attatctgct ttaaataatt ttactgacga gttatttaat acgttattaa ctttaatttc
    29821 ttggtttgta agacctgctt tatatgtcac agagtttgcg tcaattctag gtgggtcagg
    29881 tttaacttta gataaaatag ttaaatcttc tgtttgtcta ttatcatatg tcactctaat
    29941 aagacgagta gatatgccta caacatcttt gttaggacca tatgttccaa caaattggaa
    30001 attcatatgt tgcgcatttg tcgaccaaga attatttact ggcttaacat attgtttcgc
    30061 atcatacata tctgaaacac ctgggtataa aacacctctg gctgtttcaa agacatgttt
    30121 cggtacagtt cttactactt tatatgttgc tgttttcgta atcggcgttg tttcgccatc
    30181 aattaagata tgtgcagtta cagttatatc ttcaccaata cgtgtattat ctttatttgg
    30241 cgcttgtcca cttacccatg taatagttgc atctgcaatg tcactaccat tagataactt
    30301 aaagtaatct gatgcactag aaccattacc tgctgggaag tcttggcctt gtacagtgta
    30361 atgcgaatgt gcaacgattt ctggaatgac atgctttaca ggtacagtca ctgttgatgt
    30421 tgttccatct tgataagtaa cagttactgt gacattggca ttatttccag tacgactgac
    30481 attactaact gttttactag tgatttcatt cgcacttgct cttgcataac ttctatttgg
    30541 tactgtttca gtaaacgtta acgaattaat aattgtcgtt tgatcagctt gtgatactgt
    30601 cgcattattc gaaatattgg caattctcac aggattagca gccgttgatg aagtaccaac
    30661 tcgatattta tcacgcaaag gtttcactgt tacattgaac gaagttgtag ctgtattgcc
    30721 gcttgtatca gttgctaata aattgattgt cttattagtt gctgatgtca catttggtgc
    30781 cgttgcagaa acatgttgat ggttattatc aacagtacct gtaatttgtg atgtatttgg
    30841 tacagttaca gattgcacac caaagttatc attcgctgta atagtatttg taaatgtttc
    30901 acctgagaac acttgatgat tagtgtaatt acctactgaa attgttggat tcactaagtc
    30961 acttggtaca aaataaatgt taatagcgtc agtagtattt cctgtatttt gatttaaatg
    31021 ttcaacatat tgttttggac catatggcgt taagtataac tgtgctttat aaactgcatc
    31081 acttgcatta tgtgtagaat tactttttac aacgtggtca attgtaaatg cgccagcacc
    31141 attagctgcc ggttcgttta cgttaacaac agttgagtta ctatggtttg cacgagtatt
    31201 tgaatttgta acaggttgcc caccataacc gtttgaaggg ttgatgatgt ctaacacttg
    31261 aatcacttga ccattcaacg taaattgacg ttgaccatca gttgtcgcat ttgattgtga
    31321 ataaccagtc gttctaaagt taggtcttcc gccagcttct gcttgtgatt tttcattacc
    31381 agtaatgtta tttcgcaatc caggcactgt agttgttgct tttaacattg ttaaaatatc
    31441 attcgtcgta atttgagtac ctaatgtagc atcataacga taaattaaag atttatggtc
    31501 atcagaccat gataaaccac catctgttgg tctgtttgca atttttgcat tcgtccatgt
    31561 cactgtataa tcttgtggga aattaaccca acgcaagaaa ttcatattag ctaggtttga
    31621 cgcgaatgat ttcgttaatc gacctttatt aatatttact gtaatagtac ttacattagc
    31681 accattaggt gtatttgtaa ctgaaatatc accttcggca agcgtaatta catctctatt
    31741 tgcattaata aatgcttgtt ttacttttga gatttcatca gtagttaaat catatgttga
    31801 tgtacgatat acacgttgat cgccttctgg ttgtttcaca tcgatgattt tcaaactaat
    31861 atagaagtct tcactatctt tatgatacgc atttgtagca gttatatggt ataactgcgt
    31921 gcctgcgttc atttgactaa cttcttgtgc agttggaata gttctatcag atgtaatatt
    31981 tgtaaatgtg ccacttggaa taggttgtcc attacttaaa gtaaaatatt gtttaatatc
    32041 accaatacta aatgattgtc ctttagcaat cgttaccgtt gctgttttag cagcattgac
    32101 agtttggtat tcaggtttca caactggttc gtatgtcatc acatttgtag catcaagcgt
    32161 tgtcgatgag ccatcacggt aatgtaatgt gacattacca ttattatcga cagttactgg
    32221 atcagttgat gctaaatatc ctcttatatt ttggttttta ttcataaagg cagtaattat
    32281 gctttgcttt tcatcattag atacagcagt cggattgact actacaactt tctcagtatt
    32341 tcctaataca atcggatgag catcttcact aattttacct acatgaattg aaacatgttt
    32401 agattgacta tcatttgtcg tattattgac attgtctgtc gctgacactt taaatgtaat
    32461 atcactgtta aatgcctgat tcatactcac tctacctgta atagataatg agccattatt
    32521 cttatccgct tttgttaaat ttgatgtcca accattcggc aatgtcgtaa tagttgtatt
    32581 ttttatacca gaatgtctat cagagacacc cattgtaaag ttgacttcgt cgcctcgata
    32641 gtatttggca tttattccaa caggattatt gattgttggt ggaatgtcat ctggcaaaat
    32701 agtaacagca ttactcatat tagatgcaac tactttatta taataaacta ctgctctaat
    32761 ttgtttacct acaggtggta tgaatacaac ataatcttgc ggtcgtatag taattgtatt
    32821 atgcacccca tcaggtgtac cagtcgcaag tatcgtataa ccagatggta tttgatctgc
    32881 ctcactacca ccattcgtag tttgatttgt tggtgcaact aaataaacca atgccgatgg
    32941 atccaacggt attcccgaaa tattaacagg cactttttgt aatgctgtac ctcttaattg
    33001 ctcagctgtc gttgtaattg ttggtgtatg tggtttaaca cgaatttcga ataattgttc
    33061 tttacgtcca aaacgtatcg ttccaaagta atcccctggt aataagttac caacaagttc
    33121 aatattgcgt tctctggcat tattaggtct atttgtcgag ttttcaatgt gtaatgtacg
    33181 cggaatacct gtaatttgcc aatctttatc tgtaaaattc tcattacttg ttaaattaat
    33241 acgtaatggt tgaatagatg ttacgctacc acctaaattc acggaagcat tcccttggtt
    33301 acctgcatat acatagaaaa ctggatgccc agcttgtttt gaaaatggtt tttcagcaat
    33361 attttgagta tcgtcatttt taaagaattt aatattatgt tggatttcag gtaataccgt
    33421 taagttaaca ggaactaaag tttcactatt accataagca tcaggatagt cgaaaccaat
    33481 cattaatctt attgggtttc tagttcttgc tgcttcttgt aatttttgat cagtaaataa
    33541 ttgacttaat tgatattcat ttaattgttg ggctccaccc ggtctaaaag cattagtatt
    33601 tgcattattc gaataaaatc ctaacacttt attactcggc gtcttaacat aacttaaagg
    33661 atcatcaaca aaatcatcta tatttaggtt tgtactttct aatacctttt tcgtttgtac
    33721 atttccttga ctcacttcta cacgtttagt tgtacctatg cgatatgtac gattaaccac
    33781 ttgtcttaaa ccattaagac gttgttgatg gtcttgcgtt gattcgacgt atagttgact
    33841 gtacataaat aattgattgt actctaaagc acgcccacct gctgcatagt acaatttatt
    33901 aacagttgga ccttgtgttt taaatgaaat tgtataactt gcatcacctt gaccgatgaa
    33961 agtataaact ttaggaacac ccggtgattc agcaggtttt tgaccattaa tatattcaaa
    34021 attttgtcta ccaaaagctt tagtagcagg tgtcgctcca gcttcactaa aataatcact
    34081 cgctctagat aaatcttcta aagaataaat atggacagtt ggccagtcat atattacttg
    34141 gccacttcta tttcttattt taaagtcatg tgaacgatga ggatcattta ctccatattc
    34201 ccacatttgt tgaagtggtt tatttgcacc tgctcctgct ccatgactcc attgtacatt
    34261 tgttccatct gaattaactg ttacaaagtc agttcttcct actggcactt ggtctgctgg
    34321 taatgcaaac caaaagatca tattttgatg tggagcatgt cctttattaa acttaataac
    34381 ccattggtac ccgtctccag tttgtttcgc tgtcataaga gcaatttcct ttgagtcaga
    34441 ggccttcaat gtattccatg gtgtagttgg taaattatat ggcgcccctt tactagaagc
    34501 attgatatat gtaccaacag gatagcccga accatcatta gcattttcaa cataatatga
    34561 tgaatttgca ttttgatagt ctgatactga tctaggctct gcagctcttg actcaaccga
    34621 acgcgtttga attctatttg aacgtcggct agtctgttga cgttgaggca ttgtacgaat
    34681 taaagcaaca gcttgttcca attctttgta tgcaagttgc aagtctttat tgtttattgg
    34741 atcattactg cctaataacg tattaatctt atcaaaaatg ccttttacat agttaaatgt
    34801 gccagaatct gcattttcac ggtcaataaa gcgataatca tgataatttg catcaaaaaa
    34861 cgcttgtaat tcatttcggt catgttgtac gttaccatta tcagatgaat ctgaagctgt
    34921 tactacattt gcttcatgat tttctacatg agccgctcta ttgtcgtttc catttttatt
    34981 agcagattga ctttcgtttt gtttatgctt actttgttct ttatctggtt gtgttgtcgc
    35041 agccgttgcc gaatctttct ttgtatttac attttgagat gctggttgtt catcattagt
    35101 agtagatgat tgcgctactt tttgatctac taaattagct tgactaatat ttggttgctc
    35161 attttcatga gtagcagata atgattgacc attttgtgaa ttttgagaat tttgtacttg
    35221 agattctcga ttctcagtct gttcattatt actttgttgt ttctgtttaa ccacgcttgc
    35281 tggttgattt gtttcagcag catgtgcttg tgatgtattg aatcctaaaa ataccaatgt
    35341 cgcaatgaca gttgaaaatg taccaactgt atatttacga atactaaact tttgaatttt
    35401 atcacgataa ttcacagcta ttgttcactc cttatcttgt tgttatgtct ctgttgtgtt
    35461 gtgaatgtat ttacttttga ttgcttgtgt attgtaatgc tcattccatt ctcatttaaa
    35521 aatttaaaac gatgacgatt caatacaatt tcgcaatcta tccaaaaagt attgaaacac
    35581 attacaacta tgtgttgaac aatttattta tgctccagaa tataataaca caaaatatat
    35641 tgtaaggtaa attgaatttg tgacaattta taataattat aaataaagta gcgtaaaaga
    35701 gtaataaaaa accaatccct atgttcattt aaaataatga cacataagaa tcggtttata
    35761 tttttgttta atattgtagc gacttgtatg atttcaattt aattctcaat tatcataatt
    35821 gagtatcgtt ttgtttaggc acaagtaaca aaatgataac gaatgataat attcccatac
    35881 ctgcatttaa ccataatgca atcattgcac ctgtataaat gtttgtcata tttgatacga
    35941 ttgcatatac tgcaccactc aatgcgacgc caaatgctcc acctaatgca gaagccattt
    36001 tatagatacc tgcagcaacg cctacttttt ctaacggtgc atttgcaatt gctgtatctg
    36061 ttgatggtgt agcatatatc cctagtccta aaccaaagaa taaataacct ataatacaac
    36121 aaatgacata gaatatttct ggcaagaaag ttaatgaaat gagacattct ccgacaataa
    36181 gaactcctgt tccaattaac attggtttct tgcatccgag tgtttgaagt aacttttcac
    36241 caacacgaat cataattagt accattacta aataagtgat tgataaactt cctgcttgca
    36301 atgaagaata tcctaaacct ctttgaacaa atgtgttggc tactattaat gttcctgcaa
    36361 caccatttaa caaaaagttt gaagctgttg cacctgtgta agctttattt ttaaataatt
    36421 taaaatcgat taaaggattt gtagcacgct tttcaagaac tataaataaa ctaaaagatc
    36481 caattgcaat agctaataaa gtaataaaaa gaagtgaggt tacacctaat tctgatcctt
    36541 tagtaattaa aatatttaaa ctgaggagca taatgactaa aagaaccaga cctttaatgt
    36601 caaatttatt tagagaaatc gatttagatt tagtttcagg tgtgccttta ataagaaaca
    36661 gtgcaattaa tgaaattata attgatagga tgaaaatcca acgccaacct aaaagcgttg
    36721 caactgcacc tccaaaaaat gaacaaacac cagagccgcc ccatgagcca attgaccaat
    36781 aacttaaagc gcgttgtcta tctttcccaa tgtaatatga cttaataata gacaaagttg
    36841 caggcataat acatgctgct gaaagtcctt gaattaatct tcctataata agtaataaag
    36901 gaatatttga aataatgatt aataatgaac ctaatatatt taagataata ccaatgttcg
    36961 tgagtttaat tctgccatat ttatcagcaa gaccacctgc tcctactaca aacattcctg
    37021 aaaataaagc agttatacta acggcgatat taaccgttcc aatatctgta ttgaaactat
    37081 cttcaagtat tggtacaacg ttaaccaatg attgtgcaaa tagccaaaac gttattacac
    37141 ttagaacaat tcctattaac aacttattat tgccttcaaa tgcctctctt gacggctttt
    37201 ccatctctat ttgcctccct atacttttga aaaacagaca aattccataa cagaaatttg
    37261 tctgttccat agaataattg aatggttttt gattttaatg tgtaaaactt taaatttatt
    37321 agtagtccat ccatttattc tcaatgctag ttatcttacc ttatgctgct ttttgtttct
    37381 ttttatagta ataaactggt ataccaagtg ctgttattag aattccaatg attgctaata
    37441 taaattgtgt aaacagtgta ttaattaata caaatgatcc tgccaaaata gcaattaaag
    37501 gtatgatcgg atataacggt actttatatg gtcgttccat atttggttca cgttttctta
    37561 aaattattac cgcaacaaat gacatacaat agaacaacca aataacaaag attaacatat
    37621 ttgtaattgt atcaaatgct cccattgaca tcatgatgat agcgattata agttgtataa
    37681 ttgcgccaaa ccatggtgcg ccagattttg ttaattttgc gaataaatgg ctaaatggca
    37741 ataattttct ttcagccatt gcatatggta cgcgcatacc agtcatagta tagccattga
    37801 tcgtaccata aacagaaatt aatataccga ttgtaataat cttaccgcca ttttcaccaa
    37861 ataatatttt tgatgtatct gaagctgcat ttaaattacc agcaagtagt tctattggca
    37921 acgttaataa gaatgtagcg ttaattaata aatacacagc cataatacaa ccgataccaa
    37981 ctgaaatcgc taaaggtaaa tcgcgtttag gatttttaag ttcccccgca acatttccta
    38041 catgaatcca accatcatat gcaaacatag ttgctaataa accactacca attgctgtaa
    38101 agaagccatt tcctgaatta cctgtagttg gaattaatga aaaagtgata tctccagatt
    38161 gaaaaatacc aaaaattaca ataacgatga ttggaatcag tttaattact aaagtaactg
    38221 attgtaaaat tccgcctgct tttgaaccta ggaaatttat caacacaata gataacgcag
    38281 atgcgattgc tattggtatt aacgaaccta tagataaatg gaataaatta attagctgtg
    38341 tcgcaaatac gatagacaat gctgctacgt tagctggaaa ataaataaat gattgcgccc
    38401 aacctgatag gaagccccag aaatcaccgt atgtatattc tatatacttc gttaagccac
    38461 ctgtttcagg gattgcagca gcaagttctg ctgctgttaa ccccgcacaa atggtaatga
    38521 tgccgcctag gaaccataca aacaaggcca ttcctgctgt tcctgttact tctgttacgt
    38581 ttgatatttt aaagaatact cctgaaccaa taactgtccc cataacaata gcaaacgctg
    38641 agaagaaacc tatatttttt tgtaattctt taccatttga cataattaca cctcattttt
    38701 aagattaaat gttttaacct accacaccct tgcttgtatc tgcaatattc agtccatgtt
    38761 caatgacacc tgaaactcta gttaagtcaa cattcccgcc tgaaactaat gcaacaacat
    38821 ttttatcttc aagccattta ttgtttattt ttccacttaa aattgcagct gttggtaatg
    38881 cgcctgcacc ttcagtaata attttggcac gctgcattaa atctttcata gcatgttcaa
    38941 tttcttcttc agtaacaaga ataaattcat ctactaaatg tttaactact tcatatgttt
    39001 gttcaccagg aacttttaca tcacaaccat ctgctattgt gctatccact cgatgttcag
    39061 ttaaatctct cttatagaaa gactcagcca taccatgaac attctcagat tgaacaccga
    39121 taatatgaat tgaagggtta aatgatttta atgcggtggc aatacctgca attaatcctc
    39181 caccgccaac tggtacgatg actgtattca cattccaaat atcatctaaa atttctaaac
    39241 caattgttcc ttggcctgcc attacaaact tatcgtcata tggatgaacg attgtcatgc
    39301 cattttcttt cgctaattct tccatataaa gtctagtttc gttaaagttt ttacctttta
    39361 aaataacctt tgccccatag ccttttgttg cttgttgttt cgcttgtggt gctgtttcag
    39421 gcattacaat cgttgcatca atgcctaata atttagctgt taaagcaaca ccttgtgcat
    39481 ggttcccagc agatgctgcg ataatgcctt tttctttttg ttcatctgtt aagtgattaa
    39541 ttttattgct agcgcctcta aatttaaaag atcctgtgaa ttgcatattt tctaatttta
    39601 gaaatacatt ccctttagtt atactttggc ttaaatacat tgatttaatt agaggtgttc
    39661 gacgaataaa tggtttaatg cttgctttag cttcttcgat atctcctaaa cttacaatat
    39721 gtgctgtttg taatgtaact gtgttggttg tcataataat tacatccttc cgttttgtaa
    39781 atttttagtc caaactataa tgctatattt aaaatattca tttcactcaa tttcatttaa
    39841 attactatct attcgataac atttaatatt tctttataat ctaggtcatg tgatgaagct
    39901 aatccttgat ttgtcacttg tccttggtaa atgtttacac cagtacttaa ggcttcatta
    39961 tctttaattg cttgttctaa gcctttgtca caaatttcta atatataatc aatatttcct
    40021 tgtgctaatg ccattgttga agttcttggg actgctcctg gttgatttgg tacaccataa
    40081 tgaatcacac cttcttcttc atacactgga tcagaaattg tagttggtct aattgtttca
    40141 atagttccac cttggtcaat agctatatcg attaatactg aacctttttt cattgattta
    40201 accatctcac gagtaaccaa ttttggcggt ttcgcacctg gaattaaaat tgtagaaata
    40261 aatacatctg ctttcttaat ttgttctgct aaattttctg gtgttgattt gactactgtg
    40321 acatcttttt ctgcatacat atcttcaaga tatttaatgc ggtcatcgtt taactcgata
    40381 atgattactt tagcatttag tcccaaggca acatttgctg catttgttgc tgctactcca
    40441 ccaccgaaaa tcacatatgt actaccaggt atatccacat tttcatgtac accagtcact
    40501 aaagtacctt gaccaccatg ttgtgcttca gagtagtaag ctcccataat tgctgagcgt
    40561 tgacctgcta tagcactcat tggcgctaat aattctgctt ttccattttt tataatggtt
    40621 tcaccactaa tcgcagttac accaacttct tgcatttttt ctactatttc ttttgaagat
    40681 gctagatgta aaaatcccca gataatttga ttctttttga aatattgata ttcgctttca
    40741 tgaggttctt ttactttgat aacaagatca gcttcccatg cttgttcgtg agttacgatc
    40801 ttagcgcctt ctttttcata catatcgtta gaaaatcctg aaccaatgcc agcatttttt
    40861 tcaacaatta ctttatgtcc tgcatccgtt aacttacgca cattttcggg tgtgcaagct
    40921 acacgtcctt caccttgttt taattctttg actactgcaa ctaacatttg caacactcct
    40981 ctttcatttg tgaataattt cacaattatt ttatcctttg gttatgaata tgtaaatacc
    41041 attaattaat tattcatatt ttatctataa tttgttaata aatatttaat atattattct
    41101 tattcttaaa tattatgtgc aattatacca ctatatttat ttcttatact ccatttgatt
    41161 tatagttgta ttaatacatt gactcaaaaa ctaattaatc aaatatgttt tttagattaa
    41221 taaagttgta actgtactat tttgtagtgt atggtaattt atttggatga aatataattc
    41281 tttatactct attgattaat caaatatgta tctatcaaaa ttcggcttat ttattcactc
    41341 tgacatatcc aaatacgcaa aaagactatt tctaccttgt atcgatagaa atagtctttt
    41401 tatacttaat tggtttaatt tttcataaaa tcttatactt tttaaaaatg ggatgaaata
    41461 tattttcctg aaacatgtaa ttcatgttcg ttacaaattg ataaaatatg attaagcgtt
    41521 gttgcaaatg acattttctc aaaaagcgct tcactatcaa ttggtacttg tgtgtgaatt
    41581 tcagccagtc gcttcgataa atatagttca tctaaattgt cattaatctt attacgttgc
    41641 ccagctgata atgcatcaat gttttcaacc acattttcaa cgctttgata ttgctgaatt
    41701 aacttaatag ccgttttctc gccaatacct ttaacacccg catatccatc tgctgtatca
    41761 cccataaacg ctttaatatc gattagttgt tgtggttcaa gggcatattc ttcgttaaaa
    41821 cgatgtaatg tatatctatt ataaatgtta aaaccttttt taattagcca aacttcaaca
    41881 ttgtcattaa tacattgcag taaatctttg tcgcccgtaa taatatagac atcgttatca
    41941 gttgaatatt gttgtgctaa tgtacctata acatcatccg cttcatagtt tttaacgcca
    42001 atatttacaa agccaaattg ctctgaaatt tctttaacat aatcaaattg tggtatcaat
    42061 tcttctggtg gtgcagaacg attttgctta taaccatcaa acatatcatt tctaaaagtt
    42121 gattgtccca tatcccaaca tacagctaca tgtgtagggc gtatttcatg tattgccgaa
    42181 aagatatgac gcacaaatcc ttgtattcca tttgtaggtt caccttgtga attgtacata
    42241 aattgtttat gaagacttgt agcatagaaa tgtctaaata atagcgccat accatctaca
    42301 agtaatattt tattaggcat ttaaaacgtt ccttctttct taatataaaa tagttttcag
    42361 ttgttgatgt ttatctttca attgattgat aagtgttgta tctaaatcaa atgctaataa
    42421 atcgtttatt tgcgtttgaa tattagcttc aaaactttca aattgcgatt cagcttctac
    42481 agccatttgc tttacatata attcaagctg ttgcctcaat aatcccattt gtggttgtaa
    42541 taattctaac gtactttgac aaattgattc atgtatatct ctttgcccat ttggattcaa
    42601 aattttacgt tttgttaatt gtttaggcaa tgcatttagc atatcgttga aatcgatgtg
    42661 caataatggt tgctctatat tagctgattc aaagttaaac tgaggattaa taatgacatg
    42721 taaatctgct aattgttgaa cgattggtgc aatttgctca gtgagttgct tattaaagta
    42781 tttttttata cgttctgtaa ttaaagattg ctctaaaaac aatcgttgat gaatttgatc
    42841 taagtatact ctcgtagaca ctttcttttc ttcattaaaa tcactatttt gcgtcatttg
    42901 agaattaaac actgatttaa catcgtcaag tagttgtagt tttaaacggg catttaaatg
    42961 ataaacttgt tcttcaactt cgttgtctgt atgttgtaac gtcgcatcaa ttaattgatg
    43021 ttgtaaacgc tgtttatctt tataatctgt taacctttgt tggcgacgtg aaatatcagc
    43081 tttatttgtt tcaaattctg taatcatctc tacataagaa cgatccattt gttgaagctg
    43141 atgaatcatt tgttgttcta aaattgattt agattcaaca tcaacaaatt gttgtatgct
    43201 ttgttttaat tgatcaatgc ccttatcttc agcttgcaat gcatttcgac ttgatacagc
    43261 aaaaatgtct gattgtaagt gtacttgtct taatgcatct gatacatatg tttcaactgc
    43321 ttcaagatca tcttgacttt ctgctaaatc agcagcatta attaccattt ttaatgcttg
    43381 gttttcattc aactggttca tatctttcat gtgttctata aacgctttgt cattatcagt
    43441 aaatgaatga ttaaaataac ttacatacaa tattaagtct gaagaagtta aaatttgttc
    43501 ggtttcattt gtatgccttt ggttatttga gtgtagccct aatgaatcaa caattatttt
    43561 accttttaac caatcatgca ttaatgcaat gtgtactgtc ttaacaaatg ttgcatattc
    43621 atcttctgca ctccactttt tcaattcctg ttgattaatg gcatgttttt ctccattttc
    43681 caacatattg acatacaatt tataatgttt ttcaactgcc tgaataaaag cgagttgatt
    43741 tttatttaaa tgtgatttta acttttctaa atctgaatta ataaagtctt ctatagtcga
    43801 ataagacata tcttggtatt caactactgc attaatttca tctaataatt gcgattgtga
    43861 ttttagcgtt atataactct cgtctccata agatatttct gtagtagctg ctgtagtagg
    43921 atttggagaa ctgactaaaa tatgctcgcc taataatgca tttatcaaac tacttttacc
    43981 agcactaaat gttccaaata cacctatttt tattaattta ttatctatac gtgttaatgt
    44041 ttcgtggata tcttgctttg tacgcttgaa caaaggcaca tccgaaatta tatcaagcgc
    44101 tttttgaata tctatagaca tatttgttgt agctgtattt tgatttagct gctgattatc
    44161 acgattatcc tgagcattat cagtagccac ttgatatgtt gtctcttgtc gacctattaa
    44221 tttatctaga gattcatcta aatgaatata gtagtgacga taattcttag tcgtcaatga
    44281 ctgacgcagc tcatttaatt ctgtataacg ttgatattct tttaaatcat cactttcttc
    44341 tgttggtaat tcatctgcct gcacattttc tattatttct ttaaaaattg gtgttgattg
    44401 ttgttcaaca tatttcttaa tggctttaac cacttcgtct gaaaatgtaa gtacataagt
    44461 attgctgatt gatgtttgtg gttgatataa accttcaatc atttctggct taacgtcata
    44521 atgctgattt aatactttat ctgaagcttc ttttttattg ataaaacgcg taacaaatga
    44581 catatcttct cgcattggtt gacgaatttg ttgattaaca tgttcttgta acgcatctgt
    44641 cgctgttaat aatcgctgtt gttgaatttg tagttttttc ttctttttat taaaaaatcc
    44701 gccaacatta aagtcttgag acatactttc caaataactt cgtaacattt ctctcatatt
    44761 atgcggcata atgtatgcat tttctaaaat atttttacgc ttattcttta aaaatgccat
    44821 taattcatct ggattattta aaagttgtgc ctcttcacta attgcttgat gttgttgact
    44881 atttaaaaat gcttgttcaa actccgcttc ttcgatacct aaatcttcta gtacttcctg
    44941 aatttcagac tgaatgtaat ctagctgagc ttcggtaatg tattcaaccg ttcttgatgt
    45001 ataatcctct attgtctctc tatgttgatc taatgaaatt agataacttg ataatacttc
    45061 aagttcattt tcagggtgat caaatttaga tacataaaag gtgcgttcta atttaatacc
    45121 ccaatctgca attgattttt caactcgaga tttaaacgta gagaatgaca attcatcgtc
    45181 ttgatgcttg tcaatttgat taatgataaa cacaacaggt attccaacat catttatatg
    45241 cttcataaac ttaaagttaa gttcagattg aacgtggtta tagtcaaccg tataaaatat
    45301 catattactt gtatacatat attgttctgt tattgactga tgtgatgcaa catttgaatc
    45361 aacacctggt gtatcttgca acgtaaaccc attttcaaat ttagctgatt gaaaattaat
    45421 ttctacagat tcaacgtcga cattttggcg attcatttcc cttacttcat cataattaga
    45481 taatttggca tacgtttgat tcggcaaatt agcaataata tcgtgattgt ctgaaactga
    45541 cacaatagca gtattacttg tcgttggtac aggtgaactt ggtaagatat cttgttcaat
    45601 taataaattt atcagtgtcg atttacctgc agaaaaatgt ccaacgaacg aacatgtata
    45661 ttgctgcaaa tatactttct taattacttg gttaattgta tgtaaaagtg cttcatttcg
    45721 cgacttttca acttcttttt ttaatttata taaaagatct aattgttctt tattaatcat
    45781 gttactttcc cctttggttc gttcacatag aaatgcttgc ttgttacaac gaattcatac
    45841 aatatcgatg tccatttaaa catagatcgt taaatggctc gcattgtatt atgtttaaat
    45901 tttatcatag ctgcttgata aaacatacga ataatcaatt ttcttcaaac attccaactt
    45961 aaaagttcat ttttctaaaa atcacaaatc atcgttaaat ttttctagaa aataaatatt
    46021 gtcacagttt gttcacacac atcacagaat tgacaccgta taatagccaa ttgggaggga
    46081 aatatgaaaa atacatttaa tccattacaa agattacatt tctatgcagc aatatttatt
    46141 gctccactgt taatcacttt aaccatttcg ggcattggtt acttgttctt tccagaagtt
    46201 gaaaataata tttataagaa tgagtttttt ggtgacagtg atgtaaaaac gcatcaaaca
    46261 ttaaatgatg cagtacatca agttgaacaa caatatgaag gattctttgt aagtaaagtt
    46321 agcatacttg atgaaccata taacaaacga attacactga gtgatatggc aggaaatcaa
    46381 cgttacgtct ttctagatca taacaatcaa tttgttgcag atcaaaatgc gaaacatacg
    46441 tattctaatg tgatgcgaag tatacatagt tctttgttta ctgaaaatac tattattaat
    46501 tatttagtag agttaaccgc atgttggatg atattcatga ttttatctgg tacttattta
    46561 ctcattaaga agcatttaat ttctaacaaa agtaaggcac ttcgttggca aaagtggcac
    46621 gcaatgattg gagttatcat tgcaattcca gtatttgtat tagtcttaac tggattgcca
    46681 tggtctggtt ttatgggcag taaaattgcc ggtatgatgg acacaaacgg tgcccttggt
    46741 caaggtgaat tagcgattaa tccacctaaa tcagatttga acgaattacc ttgggctaca
    46801 cgtaaaaata aacagccagc ttcatccgaa aaaggttcaa gtggtcatca tggtaatgca
    46861 gcaatgcctc aaaccaaatt agattatcaa atatctattg ataaggtcgt tgaacaggcg
    46921 caaaaagctg gtattaaaaa gccgttttca atcgtatatc caagtgataa aaatggtacc
    46981 tttattgtat ctaatactag taattcaggt gttactgggc tagatgtatc accatacaag
    47041 gaacaaacac tttatttcga tcaatatagc ggtaaaaagc taggtacgat taaatatgat
    47101 gactacggta ttattgctaa atggtttaca tggggcattc cgcttcacga aggtcattta
    47161 ttcggcattt taaataaaat cattaattta tttgtatgta tcgctttatt agtagccatt
    47221 ggcatggggt ttgtctcttg gataaagcgt acaaaaaata ctgcaataaa agtaccacat
    47281 cgcgtaaaaa aaccagcatc tatatcactc ataatatgtt taattgtatt aggattatta
    47341 atgccattat ttggattatc acttatcctt gtatttataa ttgaattaat attatatatt
    47401 aaagatcgtc gtgctaaaca ataatgcact taaagttttg aactgacgaa atttacaaaa
    47461 tgattctcgt ctctctaatt acttaaaacg ggttctataa taaatcgtac tgatggaaag
    47521 tttttacttt ttatctgtcc gattttttga atttgaagat aaaaaaacat ctaaaacgca
    47581 taagaagata actctacttc ttcaaaatta cgttatagat gcttttttat taattcatat
    47641 gcatgtcgtg aattttcatc attaaaccgt gtgggacgtc atcatgttta acaatctctt
    47701 gtttataaaa tgtcttccca tcatctttag aaactaattt tacaaagtct ccttttttcg
    47761 gcttgaaatc attagatggt tctactttga tattatgttt gactaatcca tctttttcac
    47821 tgacaacttt ttctgcattt gtattactat tcatataacc aaaataagtc gttttttgac
    47881 tagaaaaaat cattaaacat acaacggcaa taccaataac aaccaatgca atagataaac
    47941 ctagacccat ttttttaccc atttatagac tccttcattt ttcaaaaata cctattatca
    48001 tgagaactta attattacta attttagtca cgcattagaa acactttagt tttcaatagt
    48061 tttacgttct gtctttttac cccagtattg ataataagta cattctatat atccattaaa
    48121 taacttacgt cgctttgttg ctttacgatc tactaaatat tcaaattctt tattacttgt
    48181 taaaatgtat gtagataaaa atggatgttg tttcattagt ttaccaatat aacggtacat
    48241 ttcttcaact tcttcacgat caccaatacg ttcaccatat ggaggatttc caattaacgc
    48301 caccggttct tctgtatcaa ttgttaatgt attgacatct tttacactaa atttaataat
    48361 atcagacaat ccaacttctt cagcgttacg cttagcaatc tctaccattt ctggatcgat
    48421 atcagaagca tatacttcga tttctttatc ataatcagcc atcttatccg cttcatcacg
    48481 gtaatcatca taaatatttg ctggcatgat gttccattgc tctgatacga actcgcgatt
    48541 aaaaccaggt gcgatatttt gagcaattaa acaagcttct atagctattg ttcccgaacc
    48601 gcaaaatgga tcaattaaag gtgtatcacc tttccagttt gcaagacgga ttaaacttgc
    48661 tgccaacgtt tctttaattg gtgcttcacc ttgtgctaat ctataaccac gtctgttcaa
    48721 accagaacct gatgtgtcga tagtcaataa tacattatct tttaaaatgg caacttcaac
    48781 agggtatttg gcacctgatt catttaacca acctttttcg ttatatgcgc gacgtaatcg
    48841 ttcaacaata gctttcttag ttatcgcctg acaatctggc acactatgta gtgttgattt
    48901 aacgcttcta ccttgaactg ggaagttacc ctctttatca attatagatt cccaagggag
    48961 cgctttggtt tgttcgaata attcgtcaaa cgttgttgcg ttaaaacgtc caacaacaat
    49021 tttgattcgg tctgctgtgc gcaaccataa atttgccttt acaattgcac ttgcgtctcc
    49081 ttcaaaaaat atacgaccat tttcaacatt tgtttcatag cctaattctt gaatttccct
    49141 agcaacaaca gcttctaatc ccatcggaca aactgcaagt aattgaaaca tatatgattc
    49201 tccttttata caggtatttt attcttagct tgtgtttttt atacatttcc aacaaattta
    49261 atcgctgata cattaacgca tccgcttact attttaaaac aaggcagtgt cattatatca
    49321 agacaaggcg ttaattttaa gtgttctttt catgaaaaaa gctctccatc atctaggaga
    49381 gctaaactag tagtgatatt tctataagcc atgttctgtt ccatcgtact catcacgtgc
    49441 actagtcaca ctggtactca ggtgataacc atctgtctac accacttcat ttcgcgaagt
    49501 gtgtctcgtt tatacgttga attccgttaa acaagtgctc ctaccaaatt tggattgctc
    49561 actcgagggg tttaccgcgt tccacctttt atatttctat aaaagctacg tcactgtggc
    49621 actttcaaat tactctatcc atatcgaaag acttaggata tttcattgcc gtcaaattaa
    49681 tgccttgatt tattgtttca tcaagcacga acactacaat catctcagac tgtgtgagca
    49741 tggactttcc tctatataat atagcgatta cccaaaatat cacttttaaa attataacat
    49801 agtcattatt agtaagacag ttaaactttt gtatttagta attatttacc aaatacagct
    49861 ttttctaagt ttgaaatacg ttttaaaata tctacattat ttgaagatgt atttgttgtt
    49921 gtattattcg aagaaaaact tttattgtcc tgaggtcttg atgttgctac acgtagtctt
    49981 aattcttcta attctttttt aagtttatga ttctcttctg ataattttac aacttcatta
    50041 ttcatatcgg ccattttttg ataatcagca ataatgtcat ctaaaaatgc atctacttct
    50101 tctcttctat agccacgagc catcgttttt tcaaaatctt tttcataaat atcttttgct
    50161 gataatttca atgaaacatc tgacattttt tccacctcat tagaaacttt gatcttcaga
    50221 ccactgtaag tcattgatga atgctgttaa ttcatcgaac gtcacaatat cacaagtata
    50281 gtttgtttta tccataaaat caactaacat ctgcttgaag aacttaggac tcgcctcttg
    50341 ttcctcatca taaatgagca atgtttgatc cgaatgttca agcataaatt gatctgcttg
    50401 tttaaattga aaaggacctt gatacgacgt atgaaaaata ctatcaacat aatctgcatg
    50461 cttaattatg ttggcatact tactttgatt atgttcattc cacttttctg tatgtccttg
    50521 gaacggtgta attacagcaa actttaacga atcatatgtt cgttgtaatt caataacaac
    50581 ttctgcagtc cataattcta tacccatttg cccttgtatt aacacccatt ctaatccttc
    50641 atccaacagt tgttcaattt tatgttttat aaattgtttt aaataatgta cttcaggtgc
    50701 gtcatcttta aaaatgttta attcgaatga tttgtaacct gttacataaa ctgttttaac
    50761 catcagtacc ctcttttgtt acgccatcta atatattttg taaatcatat tgaacacttt
    50821 taagcttttc tataaataac tttcgacttg ttcgcttaaa atgacattcg acagaaagtt
    50881 gttcaatgtt agcaatcagt agctcaaatt tccttgaatt catataaggt acttctataa
    50941 taaattcacg atgtaatttg atctcattga gtatgctatc aatatgttca gtatatggct
    51001 ttacagtttg gtaaaaatca tgatcttgtt gttgtgattt cacattttca aaattttgtt
    51061 gcatgttgtt aacttcataa attagtgatt cgactacatc attcatgagg acgcctccta
    51121 cattttttaa tttatcacaa tatactgtat tcgtcatgtt ttaacactct atataatttg
    51181 atttaactat tttttcaaat gtgttatctg ttaaatcaag taaatctaaa aacttcctat
    51241 ataaatacaa aattttatcg tgtatgttgt tatacgatga aaatactttt aatctaataa
    51301 aatcatttaa atcaaataca cctctgctga ttaacaacac atacttgtac ttgcctcaaa
    51361 aataaaaatt actaatcatg atttgacttt tataac
//
LOCUS       DALAAR010000009        48274 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000009 DALAAR010000000
VERSION     DALAAR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.009"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            254..958
                     /locus_tag="O2458_000532"
     CDS             254..958
                     /locus_tag="O2458_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ3886420.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1351..1887
                     /locus_tag="O2458_000533"
     CDS             1351..1887
                     /locus_tag="O2458_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886421.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(2000..3541)
                     /gene="guaA"
                     /locus_tag="O2458_000534"
     CDS             complement(2000..3541)
                     /gene="guaA"
                     /locus_tag="O2458_000534"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCZ3886422.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3566..5032)
                     /gene="guaB"
                     /locus_tag="O2458_000535"
     CDS             complement(3566..5032)
                     /gene="guaB"
                     /locus_tag="O2458_000535"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCZ3886423.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5070..6338)
                     /locus_tag="O2458_000536"
     CDS             complement(5070..6338)
                     /locus_tag="O2458_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCZ3886424.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6338..6916)
                     /gene="xpt"
                     /locus_tag="O2458_000537"
     CDS             complement(6338..6916)
                     /gene="xpt"
                     /locus_tag="O2458_000537"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCZ3886425.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7147..7249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7429..7836
                     /locus_tag="O2458_000538"
     CDS             7429..7836
                     /locus_tag="O2458_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCZ3886426.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7979..8641
                     /locus_tag="O2458_000539"
     CDS             7979..8641
                     /locus_tag="O2458_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886427.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8759..9715
                     /locus_tag="O2458_000540"
     CDS             8759..9715
                     /locus_tag="O2458_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886428.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10506..10607)
                     /locus_tag="O2458_000541"
     CDS             complement(10506..10607)
                     /locus_tag="O2458_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886429.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10692..12080
                     /locus_tag="O2458_000542"
     CDS             10692..12080
                     /locus_tag="O2458_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCZ3886430.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12161..12916)
                     /locus_tag="O2458_000543"
     CDS             complement(12161..12916)
                     /locus_tag="O2458_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCZ3886431.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13408..13977
                     /gene="ahpC"
                     /locus_tag="O2458_000544"
     CDS             13408..13977
                     /gene="ahpC"
                     /locus_tag="O2458_000544"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCZ3886432.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13993..15516
                     /gene="ahpF"
                     /locus_tag="O2458_000545"
     CDS             13993..15516
                     /gene="ahpF"
                     /locus_tag="O2458_000545"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCZ3886433.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15646..15888
                     /locus_tag="O2458_000546"
                     /pseudo
     CDS             15646..15888
                     /locus_tag="O2458_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15961..16587
                     /locus_tag="O2458_000547"
     CDS             15961..16587
                     /locus_tag="O2458_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCZ3886434.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16842..17225
                     /locus_tag="O2458_000548"
     CDS             16842..17225
                     /locus_tag="O2458_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886435.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17291..17872)
                     /locus_tag="O2458_000549"
     CDS             complement(17291..17872)
                     /locus_tag="O2458_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ3886436.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            18050..18259
                     /locus_tag="O2458_000550"
     CDS             18050..18259
                     /locus_tag="O2458_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886437.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18298..18549)
                     /locus_tag="O2458_000551"
     CDS             complement(18298..18549)
                     /locus_tag="O2458_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCZ3886438.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            18849..19112
                     /locus_tag="O2458_000552"
     CDS             18849..19112
                     /locus_tag="O2458_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3886439.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(19250..19822)
                     /locus_tag="O2458_000553"
     CDS             complement(19250..19822)
                     /locus_tag="O2458_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ3886440.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(20003..20371)
                     /locus_tag="O2458_000554"
     CDS             complement(20003..20371)
                     /locus_tag="O2458_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ3886441.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(20740..21351)
                     /locus_tag="O2458_000555"
     CDS             complement(20740..21351)
                     /locus_tag="O2458_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ3886442.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            21551..22258
                     /locus_tag="O2458_000556"
                     /pseudo
     CDS             21551..22258
                     /locus_tag="O2458_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            22297..23235
                     /locus_tag="O2458_000557"
     CDS             22297..23235
                     /locus_tag="O2458_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ3886443.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(23501..23743)
                     /gene="rpsR"
                     /locus_tag="O2458_000558"
     CDS             complement(23501..23743)
                     /gene="rpsR"
                     /locus_tag="O2458_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCZ3886444.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(23795..24298)
                     /gene="ssb"
                     /locus_tag="O2458_000559"
     CDS             complement(23795..24298)
                     /gene="ssb"
                     /locus_tag="O2458_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ3886445.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(24319..24615)
                     /locus_tag="O2458_000560"
     CDS             complement(24319..24615)
                     /locus_tag="O2458_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCZ3886446.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            24859..25050
                     /locus_tag="O2458_000561"
     CDS             24859..25050
                     /locus_tag="O2458_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886447.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(25136..26233)
                     /gene="ychF"
                     /locus_tag="O2458_000562"
     CDS             complement(25136..26233)
                     /gene="ychF"
                     /locus_tag="O2458_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCZ3886448.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(26245..26448)
                     /locus_tag="O2458_000563"
     CDS             complement(26245..26448)
                     /locus_tag="O2458_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCZ3886449.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(26478..27359)
                     /locus_tag="O2458_000564"
     CDS             complement(26478..27359)
                     /locus_tag="O2458_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCZ3886450.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(27516..28361)
                     /locus_tag="O2458_000565"
     CDS             complement(27516..28361)
                     /locus_tag="O2458_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCZ3886451.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    28590..28959
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            29025..30128
                     /locus_tag="O2458_000566"
     CDS             29025..30128
                     /locus_tag="O2458_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ3886452.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            30125..31285
                     /locus_tag="O2458_000567"
     CDS             30125..31285
                     /locus_tag="O2458_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCZ3886453.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            31254..33095
                     /locus_tag="O2458_000568"
     CDS             31254..33095
                     /locus_tag="O2458_000568"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCZ3886454.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            33092..35320
                     /gene="metE"
                     /locus_tag="O2458_000569"
     CDS             33092..35320
                     /gene="metE"
                     /locus_tag="O2458_000569"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCZ3886455.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            35363..36115
                     /locus_tag="O2458_000570"
     CDS             35363..36115
                     /locus_tag="O2458_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCZ3886456.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(36198..37379)
                     /locus_tag="O2458_000571"
     CDS             complement(36198..37379)
                     /locus_tag="O2458_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCZ3886457.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            37918..39051
                     /locus_tag="O2458_000572"
     CDS             37918..39051
                     /locus_tag="O2458_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCZ3886458.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(39418..40047)
                     /locus_tag="O2458_000573"
     CDS             complement(39418..40047)
                     /locus_tag="O2458_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCZ3886459.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(40047..40889)
                     /locus_tag="O2458_000574"
     CDS             complement(40047..40889)
                     /locus_tag="O2458_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886460.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(40914..41627)
                     /locus_tag="O2458_000575"
     CDS             complement(40914..41627)
                     /locus_tag="O2458_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCZ3886461.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(41624..41827)
                     /locus_tag="O2458_000576"
     CDS             complement(41624..41827)
                     /locus_tag="O2458_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3886462.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            42068..42457
                     /locus_tag="O2458_000577"
     CDS             42068..42457
                     /locus_tag="O2458_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCZ3886463.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            42557..42781
                     /locus_tag="O2458_000578"
     CDS             42557..42781
                     /locus_tag="O2458_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCZ3886464.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAISS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            42798..43499
                     /gene="tatC"
                     /locus_tag="O2458_000579"
     CDS             42798..43499
                     /gene="tatC"
                     /locus_tag="O2458_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCZ3886465.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(43591..45303)
                     /locus_tag="O2458_000580"
     CDS             complement(43591..45303)
                     /locus_tag="O2458_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCZ3886466.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(45284..46513)
                     /gene="efeB"
                     /locus_tag="O2458_000581"
     CDS             complement(45284..46513)
                     /gene="efeB"
                     /locus_tag="O2458_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCZ3886467.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(46510..47364)
                     /locus_tag="O2458_000582"
     CDS             complement(46510..47364)
                     /locus_tag="O2458_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886468.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(47632..48177)
                     /locus_tag="O2458_000583"
     CDS             complement(47632..48177)
                     /locus_tag="O2458_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3886469.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
BASE COUNT        15938 a         8998 c         7200 g        16138 t
ORIGIN      
        1 ttagattgct tttagcatta ggaattcgct tataattaga tgttcaatat agactttttt
       61 acacatacat gaactataaa aagcgaataa atagcaggtc attgcgaaat ttgctaaata
      121 caaaattgat gagttccacc taataaaaat tgttggtttt atagtaacat ctattttcgt
      181 accattcatg ttaatttcag tgctaataat gctgatataa agaagaataa attgattagg
      241 agaacactat ttaatgtcta aaaatatcaa tcaagcaaat agtaaattaa atacgtcaaa
      301 caaaaaacta aaacaagctt attcaaaatc cgacagtaaa aacatgaaag ttatatacat
      361 gcctcattgg ctagagtttt attcaattgc tatacatagc gatgttacaa gcaataaaaa
      421 aagatattat aaatcatata gtagaggaac agtggtatat gtaaaactag ggagtaatat
      481 tggaagtgag ttttctggta atcatttttg tgtaatatta gataacaaag acaataaagg
      541 aaaagaaaca gttacaatag ttccactttc ttccaaaggt aataaaaatt atttgaagtt
      601 aaacgaatcg gtgctaaatc taacagcaac agatttaaaa aaacagatta tcgatatcag
      661 tagtaaagta caagcattag cccaaaaata tagagaaatt gacattaaac ttagcacaga
      721 agataaaaag cttttgtcta acttaaatca aaaagctaat gaactgtata gagtgatagg
      781 tgtttattcc aagcacaaag ggaaagatac atacgttaat atatcagcaa ttacaacaat
      841 aagcaaaaga cgtataagca aaataaatga ttctgatcct actggaacga taataatttc
      901 agaagatgac atgaatgaaa tcgaaaagca attaaagata aagttctttt ctaattgaat
      961 tgtaatatta catctgatat aattgattta caaattcccg gtaaccattc cggcttcatt
     1021 ctcttgaaga atgacatatt ctcagcgttt tagctgaagg tcagatgata cgtcatctgg
     1081 cctctttttt tatataaatg ccctttaagt gaaatataca acaatctgca atcaatatta
     1141 gacatcacaa aacacacaat aaattacaca atgtgttata agccacatgt cactattatc
     1201 gcagctagct gcgtcactca aaacgaatac ggaacgcgtt ggttatttca atcataatat
     1261 tactctgcaa atacacagcc ctgtaaccga gtaaatggat agagatttga acaaatgaaa
     1321 acaatcaact aatggaaagg ataaaatatt atgcacaaca ataatgaatt caacaaaaaa
     1381 ctgaaagatt ttataggtag cgataaacgg atggctttag taaagggtta tgttaacgag
     1441 tataaattaa caactgtttt aagagcttta aataattccg attacacaaa agggacgata
     1501 cacactcgtt caataggcaa cctaaaacaa atagtagata atcgaattat tcctaaaaat
     1561 ataactcaaa acacaatctt caaattgagt aatttaacct tagaggttaa cctctatgaa
     1621 agaaacacaa tttatcacgg agactttgct atttactatc ctgtagaatc tgctctaata
     1681 atagaaacag atactcctag gctaattaat catataaata acaacccagt ccaaaaaata
     1741 tttataataa caacaaatga ctggagcttt aatacacttg aattagaaaa aatagtagac
     1801 gaaactataa tttatgattt aaaacaagaa gatcctaaac aatatgaaat tttatataaa
     1861 aataaacacg gaaaattgcc ttattgatga attaaaacgc aaacttagaa caccttaccc
     1921 cttttccgtc cttattgtaa ttttccaact actcaaatta aaaaccaccc gtgcaaacag
     1981 gtggttttat tatatataat tattcccact caattgtgct tggtggtttt gatgtaatgt
     2041 catagactac gcggttgacg tgatctactt cgtttacgat acgactagaa atcttttgta
     2101 agacttccca atcgatgcgt gcgaagtcac ttgtcatacc gtcgatagat gttactgcac
     2161 gaatacctac tgtgtgatca tacgtacggt agtctcccat aacacctact gattgaatgt
     2221 ttggtaacac tgtgaagtat tgccaaattt ctctttcaag accttcttct ctaatcactt
     2281 ggcgtaaaat cgcgtctgat tctctaacga tttctagttt atcttcagta atttctccaa
     2341 gtacacgaat accaagacca ggtcctggga atggttgtct ccatactaaa tgttctggaa
     2401 tacctaactc aatacctaat ttacgtactt catctttaaa caatgtattg attggttcga
     2461 ttaattcgaa ttccatgtct tctggtaatc caccaacatt gtggtgtgat ttgattgttt
     2521 gtgctgtttt agtacctgat tcgatgacgt ctgtatatag tgttccttgc gcaaggaagt
     2581 ctacaccttt cagttttgat gcttcatcat caaatacgta tacaaattca ttaccaatga
     2641 ttttacgttt ttgttcagga tctgaaacac cttttaattt attcataaag cgatctttcg
     2701 cattaacacg aataatattc atgttgaaac cttcaccgaa ttgctccata accatgtcgc
     2761 cttcaccttt acgaagtaag ccatggtcta caaagataca tgttagttga tcacctattg
     2821 ctttatgcaa tagtacagct acaacagatg aatctacgcc gccactcatc gcacataata
     2881 cacgacggtc tcctacgcgt tgacgaatct tttcaatttc gatttcgata aagttttcca
     2941 ttgtccattg acctctacaa tcacaaacac gacggacaaa attatttaat aaatcattac
     3001 catattctgt atgacgtact tctggatgga attgaacacc ataaatgcga cgtttcttat
     3061 cttcgattgc tgcatagtct gtgcttgggc tatcagcgat aacttcaaag ccttctggaa
     3121 tttcaataac tttatcagaa tgactcatcc aaacagtttg ttctgctggt aagccagcga
     3181 ataactcatc tgacttcgca ttaatgattg ctttaccgta ttcacgttca ttggcacgtt
     3241 caactttacc acctaataat ttagtagtta attgcatgcc gtaacaaata ccaagtactg
     3301 gaattcctaa attatatatt tccggatcaa ttgtaaatga accttcttca taaactgaat
     3361 ttggaccacc tgataagata ataccttttg gattcatttt cttaatttct tcaattgaaa
     3421 tttcatgatc gtgtaattca ctataaacgc ccatttcacg aattcggcgt gtaattaatt
     3481 ggttgtattg gctaccaaag tctaagacaa ggattaactc ttgttctttt gccatttcca
     3541 tatttgtcgt tctcctttat cttaattaga atgagtagtt cggtgattct ttcgtaattt
     3601 gaatattatg tggatggctt tctgctaaac cagcaggacc catacgtgta aattgtgctt
     3661 cttcgcgtaa ttctcttaaa tcgtgtgaac cagtataacc cataccagca cgcacaccgc
     3721 ccattaattg gtaaattgta tcttgtaacg cacctttata agccgtacgt ccttcgatac
     3781 cttcaggaac aaatttctta ggcgctttgt cctcttggaa gtaacggtcg tttgaacctt
     3841 tttccatcgc acctaaagag cccataccac ggtatacttt atattgtcta ccttggaaaa
     3901 tttctgttgc gcctgggctt tcttcagtac ctgctaataa gctacctaac ataaccgcat
     3961 gtccaccagc agctaatgct ttaatgatat ctcctgagaa tttaatacca ccatcagcaa
     4021 tgatagcttt accatgtttg cgtgcttcag ttgcacaatc ataaattgct gtaatttgtg
     4081 gtacaccaac acctgctaca acacgcgtcg tacaaattga acctgggcca ataccaactt
     4141 taacaatatc tgcacccgct tcaaataaat cttttgttgc ttctgcagtt gctacgttac
     4201 ctgctactaa tgtgatttct gggtaagtct tcttaatatg tttcacttga tcgataacac
     4261 ctttagagtg accatgtgct gtatcgataa ctaagacatc cacacctgct tcgactaatt
     4321 tttgagcacg aatatcagta tcttttgaaa taccaattgc tgcggctaca agtagacgac
     4381 catgttcatc ttttgctgca ttagggaatt cgataacttt ttcaatatct ttaatagtaa
     4441 taagaccttc tagacgtccg tctttaacta atggtaactt ttcaatctta tgtttttgga
     4501 gaattttttc tgcttcttca agtgttgtat tcactggagc tgtaattaaa ttttcttgcg
     4561 tcattacatc tacaatttta atcgagaagt cttcaataaa acgtaagtca cggtttgtta
     4621 aaatacctac taagttgcga tcttctttat tatcaacaat tggtacacct gaaatacggt
     4681 atttacccat taatgcttct gcttcataaa cgctttcttc tggcgttaag aaaaatgggt
     4741 ttgaaatgac accattttct gagcgtttta ctttttgaac ttcgtccgct tgttcttcaa
     4801 cgcccatatt tttatgaata acacctaaac caccttgacg agccatagca atcgccattt
     4861 tagattcagt tacagtatcc ataccagcag aaataactgg aatatttaat ttaactttgt
     4921 ctgataattg tacgcttaaa tcaacgtctt tcggtaaaat atcagattgt gctggaatta
     4981 ataacacatc atcaaacgtt aatgattctt ttgcaaattt actttcccac attaaaaaca
     5041 gcctccattt ttcaaattaa ttagttatat tatttcacat tttcttccgt ttgtttatac
     5101 tttattccat taaaaaagaa attaagaata attgcagaga ttgcgcctaa aacaattccg
     5161 ttttgagtca accatgcaaa ttgttcacct aaacctttaa atgcttgtgg tacagcgctt
     5221 ataccagtac ctaatcctac tgatacagcg ataattaata aattgttttg atttttaaaa
     5281 tcgatatgtc ctaatatact aacaccatat gccattacca tgccaaacat agctatcatc
     5341 gcaccgccta acacaggtag cggtatgata tttgctaatg cgccaagctt aggtatacaa
     5401 ccacatataa gtaataacac gaccatgccg tatataacat tgtttttctt agcgccggat
     5461 aaagaaacaa gtcctacatt ttgcgaatag gctgtatacg gaaatgaatt gaatatagaa
     5521 cctaacacta tcgctagacc ttccgcagta taacctttac gaaaatcttt tctttctaac
     5581 ttcttaccgg taatttcact taacgcatga tagacacctg tcgactcaat taaactaacg
     5641 atagctacaa taaagaacac taacgtcgat gtcacatcaa agctaaatcc agagaatcta
     5701 aacggcactg ggatgcctaa ccaaccggca tgattgactt gattaatatc gaccatccca
     5761 agtaagccag cacctatcgt tcctaaaacg agtccaatta atatggcaat actcttaata
     5821 aatccagttg tgaatctttg taaaagaaga ataatgatta atgtcattaa acctaacaaa
     5881 atgttcttaa catctccata gtcctttgca ccttgacctc cagctaagta attcattgct
     5941 actggcatta aattgatacc aatgatagta acaacactac ccgttactac tggtgggaag
     6001 aattttacaa gatgtgaaaa gaaaggcgcg atgataataa ctaatatccc tgataaaaat
     6061 agcgaaccat aaagtacatc tattcctttc gtttgaccaa ttaaaatcat gggcgcaaca
     6121 gccgtgaatg tacatccaag aacgattggt aatcctgttc ctgttacttt attggcttgt
     6181 aaaaatgtgg caaccccaca cataaatata tctactgtaa ctaagtaagc gatttgttca
     6241 ggtgtaaact tcaaacttgt accaacaatg attggaacta agatagcacc tgcgtacata
     6301 gctaaaagat gttgaacact taggattaaa tttttcatta ttcttctccc accaatgtca
     6361 ctttgtttcc ttctagtgaa gcaaccttgc agagagaaga aactgttaaa cctgcttctt
     6421 ctaaacgttg atgcccattt tggaaactct tttcaacaac aataccaata ccagctgtct
     6481 tagcattcgc ttgctgtgcg atatcgtata atcctaatga agcatcacca tttgctaaaa
     6541 agtcatcgat gataagtaca gtatcttctt ctgataaaaa ctcttttgaa acaatgaccg
     6601 tacttgtttt atttttagta aatgaatgaa tagatgtttc ataataacca tccgtcaaag
     6661 tgctaggttt tgcttttttc gcaaataaac atggcacatc aaaatgcagt gcagccatga
     6721 ttgcaggtgc gataccggaa gcttcaatgg ttaagatttt agtaatccct ttatctttaa
     6781 attgctcgta aaaagtgcga ccaacttcat tcattaactt tgcatcaatt tgatgattta
     6841 aaaatccatc gacttttaaa atcttctcat caatgacaac gccgtcttcc tttacttttt
     6901 gtcctagtaa ctccactctt aaaacctcct cagatttgtg tgaaacattt tgctcttaaa
     6961 ttggtgctag atacaaaaaa atccccaaac taaataatag tttcagggtt tatgagtgaa
     7021 cgaacatgca taacgaattt gtcatgcaat caatgtaaaa gaagtttcat caaataaact
     7081 gtgaccatca tataaaatga tataaatcac ccaccatggt tacaatttaa tggctgaagc
     7141 tactcctagt attgtgttgt tactcatagt catgtcgttc aaggcaacat ggtagaaact
     7201 tctaaagcca tattctttag attatatgag tttatgtaaa ttatttaacg ataatagcaa
     7261 attttcggca ttttttcaat aactgcttag gtaatctttt aatagtttta taaatgtaca
     7321 gagacgttta atgcgaatga agacttataa cttaataatg taagggtttt actttataat
     7381 aagaagggta aaagattaaa taaacaaatt tttaaagggg cgacaactat ggcagatatt
     7441 actgtagtaa atgacactgg cgaattatac aacgtaatca atcaaaagaa atctgaaggt
     7501 tatttagaat cagagctaac aattattagt aaaagtaaat tacacctaaa tgacttacac
     7561 gactctgaaa tctcccttat ctcaacaagt ggaacattta gtgatagaat gactaaactg
     7621 ttaacaggag aagatggtga acatgcagtt ttatcacgct acaacttagc gcctgatgaa
     7681 ttagaaaaat ataaacaact tattttagac gataaaatgt tagttgtagc ggttcgtgac
     7741 aaatcatcac atcaagaagt gcatgaaaat aattcagctt atgaagagat tgatattaca
     7801 cattttgctg aagcatctaa aggtcccaaa gcttagttat cacactgaat tcaaaatgag
     7861 attactgtac tgtctaattt aagatagtac agttttttat accatttaaa agtttatgaa
     7921 cagtctttta cagcaggcga ttatgctata atattattta aattggggag gtgttcttat
     7981 ggaatttaaa accattcaat ctgtcaaaga tccacattac aacgaagcac ttaaacttta
     8041 cgatactaag ttaaatttat ctttaactga agataaacgt atcatcaaac gctcattaga
     8101 aaacaacaaa accgaaaacg attatgtctt tatcgtaggc ttagaaaacg atgttgttgt
     8161 cagtctagca actgcacatt atgaagccac aaccaattca gcatttttaa tttatttatt
     8221 cgcaaaagac ctacccaacc atgatgagtt aatgacgcaa acattacatc aaattgaaca
     8281 tgaactaaac ttattatcaa accttgttca ttctagaagt attaactttt taatgatgga
     8341 agtccctaaa gaacctgcag atttaacaca agataacggc ctcattattg aacaccgtag
     8401 acagttttta tacgaacatc aatttgaaag acaaactgaa attgattaca ttcatccaac
     8461 taatgatggt cacactaaaa aagtggactt atttttaaag aaaaatatta aactgacaaa
     8521 ggatatctac ggtacaagtg ttaaatcaaa ttatattttg aagtatgtat ttgccaatgg
     8581 tatcgcacgt gaaattattt atgctttatt agaagaaatg gatttacgta aaccatttta
     8641 aacaaacttg aaatcactaa caataacgtt taaactatgt atgaagcaag actgacgttt
     8701 cactgcccgt agacaaacgt cagtatacat aaagtatatg cagaaaggac tttaaagtat
     8761 gttaactaaa gaatttgcac aacgcgtaga actaagtgag aagcaagtac gtaaaattgt
     8821 tcaacattta gaagaacgtg gttaccaact aagcaagact gaatatcgtg gtcgtgaagc
     8881 aactgatttc aaagaagaag atatcgagct tttcaaagac attgccgaca aagtaaaaca
     8941 aacaaatagt tatgatctag cgtttgatga attagaaaaa gaaaaagact tcctgcaagt
     9001 cattgtcaaa aacgatgaca aaaacttacc tactaatcaa aatgtcgctc aactagtaga
     9061 agatttacgc ctagaaatcc agaaaatgcg cgaagaacgt cacctacttg gtcaaatgat
     9121 gaatcaagta catcagcaac aacaagaatt aaaagaactt caaaatcaac ttacatctaa
     9181 aatcgattca aatagcgaat ccttaaaagc catccaaaca tcacaagagg ctatccaaga
     9241 agcgcaagcc tctcaagcaa aagcattagc tgaatcaaca aataaagttg aaaagaatgc
     9301 tgttactgaa gataaagctg acagtaaaga ttcgaaggtt gctggagtga atacatcaac
     9361 agacgctaag acggatacta aagcagaaaa tgcaggcgat ggcactgcta ctaaagtaga
     9421 taaagaggat caaatttctg caactgaagc aattgaaaaa gccagcgttg agcagtctaa
     9481 aaatgacaat gcagctgaga cttcaaataa agaagctaca gtagatgcag aagcgcaaca
     9541 tgatgctgaa caacaagtag cagaagccca tgcagaagct tctaaacaag ctacttcgaa
     9601 cgattcattg gaagcgaaag cagaaaatga cagcacagct tcacagtcag aaatgtcaga
     9661 accaaaacct caagaagaga aaaaaggttt cttcgcacgt ttattcaacc tgtaaacttg
     9721 tgatttgaac acaaatttaa tactcaattc atatatagct ttgatttaaa aactgatttt
     9781 tataaaccta tagaactgta atttactttc aaatttaata ctcaattcat aaaacctgaa
     9841 acaagctgat atactccatt caatatctac tttgaagaga gcatatcaaa ctaatttgtt
     9901 tcaggttttt taattattca ttatgattaa tattgaattt tgaataaatt tttatagtat
     9961 gttactttta aatgggatgg cttctgtgta cttctaaata gattcatctt aataagtcca
    10021 ttcaatttaa atgtttggct gtgttagtaa ctgacgaatc gcacaagtgc acactcatat
    10081 tcacatttac tacaaacgcc tttttgctca aacaaaaata tttgtttacg gtagcttttg
    10141 aactaccacc tgcacacacg aacactcaaa agatgctaat tttgattcga cagaattttt
    10201 tacaacgtgt ttgttcgaaa cgtcttttca aggttgatca tttattatct tttcattcaa
    10261 taaatatgag gttagcaatt tttatcaaac cgccatagcc acatctcgat tcaaggataa
    10321 atttgttttc tcatatctta tctctcatga cagtgcacga tatatatctt ttccaaatat
    10381 tcatcacttt tttcctgtta aaatcatttc tattattaat gctttaatga caacttattt
    10441 aatttattgc atttaatgcc ctgcttcatt ttcaaatatt aactttaatc gactagctca
    10501 tcacactatt taccctttcc acctatcact actgaattca aaacgatttg tttacaaatc
    10561 cccttatgcc aacggtcgaa tttcatcatg tttacattta attgcaaatg ccctcttgaa
    10621 atattttaaa attaatatta ttcttagcat actagtcgga attatattca aaaatttgag
    10681 gagtgacgtt catgaatgct tttcttacac tgattaatat tattgtattg gtgattttca
    10741 tcgtaatatt gcacatgatg gctcgcaaac atatatcttt tgcgaaacgt gtgtttacag
    10801 ctttagggat tggtatagta ttcggtgtgt tattgcatct tatttatggc acacattcga
    10861 atgttattac atcaacaagt gactggttca acattgtcgg acaaggttac gttgcattgt
    10921 tgcaaatgat tgttatgcct ttaattttca tttctattgt agctgcattc actaaaattc
    10981 aaatcggaga aaagtttgct aaaattggta gtctcatttt tatcttttta attggcactg
    11041 tcaccattgc tgcgattgtt ggtgttgtgt atgccttggt gtttggttta gatgcatcaa
    11101 caattaacct tggtaacgct gaacaagcac gtggaagcga aattgctaaa caagcgaaag
    11161 atttaacagc acatacgtta ccacaacaaa ttttggaatt attgcctaaa aatccattct
    11221 tagatttcac tggacaacgt gcaacttcca cgattgctgt cgtgatattt gcttcgttta
    11281 taggatttgc ttatttacgc gtagcacgca aacaacctga tcatggtgaa ttgcttaagc
    11341 gtgcgattga cgctatctat tcactagtaa tggcgattgt gacgttcgtc ttacgtttaa
    11401 cgccatacgg tgtattagca attatggcca acacattatc gacaagtgac tttggcgcca
    11461 tttggacttt aggaaaattc ttaatcgcat cgtacgctgc attaattacg atgtatatca
    11521 ttcacttaat tattttaagc ctgttaggta ttagcccaat tcgctatgtt aaaaagactt
    11581 tggaagtatt aatctttgca ttcacatcac gctcaagcgc gggtgcctta ccattgaatg
    11641 tacaaacaca aacaagacgt ctaggcgtgc cagaaggtat tgccaatttc gctgcgacat
    11701 ttggattgtc gattggacaa aatggttgtg caggcattta ccctgccatg cttgcaatta
    11761 tggtcgcacc cgtcgctaat gtagaaattg atttacaatt tattgtaacg ttaatcgctg
    11821 tcgtgattat tagttcattc ggcgttgctg gcgtcggtgg cggggctaca tttgcatcca
    11881 tattggtgtt gtcaacgtta aacttgccag tcgctttagc cggcgtgctc atctccgttg
    11941 aaccattgat tgacatgggt agaacagcat taaacgtgaa cgattcaatg ctagctggta
    12001 caggtaccgc caagttaact aaacattggg ataaagatac tttcgattca aatgataatg
    12061 ctgcattaac ttcacactaa ttcattgcaa gaaatacccg ccccctctag ctatacttat
    12121 cattttaagc tagagggggg cgggttattt tggtatcttg ctatcgctgt attaagcctg
    12181 atttttgcaa ttgttctaac atatctaatc ttgctttgtt tcctaggaac atctcaattt
    12241 gctgcgacca tgtttctttg cgattcccgt ttgtacgttg atcatagtac tcgctgattg
    12301 tctggtcgta atctgccatt tgtgcatact gtgtttcctt atcagcatga tacttattat
    12361 gatggaagac atggtcaaat ggtaagcgtg gcttggctgc accattttcg tcatctgcgg
    12421 gttcccctac tgccatacca aataccggga agacatagtc aggtaagtct aaaatttctc
    12481 gaacgcgttc aacatcattt cttaatgatc ctaaaaagac aatgccatac cccatatctt
    12541 cagcagttac cgcaatattt tcggcaacta atgctgcatc gattgcacct actagcaaac
    12601 cttccgttga accatatgca ttttccatat cagtttcagc atgttgatca actaaatgat
    12661 gacgataata atcaataaca aagacgaata aatagccatt ttctacaaca taaggttgtc
    12721 cagaaacttc tcgtaaattt tctttaatct tctcatcgtc gataccaata attgagtatg
    12781 cttgcaggaa acttgacgtc gaagcgcttt gtccagcttc taccaatttc ttaacaacgt
    12841 cttcacttaa aggtttattc ttaaattttc taacagaatg atgctttttc acaagattat
    12901 atacatgttc tgacacgtta cccactcctc gatactttac aattaattta aaatcttcat
    12961 attgcaattg tacacgtcca aactttttta tagcaattat ttgcttataa gttaataatt
    13021 gaacttttta aaatattgtc tcatacttta aaaatccagt ttctcactca ttaccttcat
    13081 ccatctcgaa caaatattct cacaccatta tcaataaggt tgacttagtt gagaatacaa
    13141 atcttctttt tttacatttt ttacattaaa atcaagatat taaatataga cacaaccttt
    13201 taaatcatca actacaaaat ccattatcaa agcactttat attatctttc tttattttaa
    13261 aagtaatcat tcactttaca aagttatcca gttgactatt ctggatcaaa atgtaataat
    13321 gctaactaag agattagaat tattataatt aagcgcaatc aattgatagt tctaattcat
    13381 acttaaattc ttaggaggaa gatatttatg tcattaatta acaaagaaat cttaccattt
    13441 acagcgcaag ctttcgatcc aaaaaaagat caatttaaag aagttacaca agaagattta
    13501 aaaggttctt ggagcgtagt atgcttctat cctgctgact tctcattcgt ttgtccaact
    13561 gaattagaag acttacaaaa ccaatatgaa gaattacaaa aattaggcgt aaatgtattc
    13621 tcagtatcaa ctgatactca cttcgtacac aaagcatggc atgaccattc agatgcaatt
    13681 agcaaaatca cttacactat gattggtgac ccatcacaaa caatcactcg taattttgat
    13741 gtattagatg aagctactgg tttagctcaa cgtggtacat tcattatcga cccagacggt
    13801 gttgtacaag catctgaaat taacgctgac ggaattggcc gtgacgctag tacattagct
    13861 cacaaaatca aagcagctca atatgttcgt aaaaaccctg gcgaagtatg cccagctaaa
    13921 tgggaagaag gcgctaaaac attgcaacct ggtttagatt tagtaggtaa aatctaagga
    13981 ggcattacaa ccatgcttaa tgctgattta aaacaacaac ttaaacaact attagaacta
    14041 atggagggca acgttgaatt cgttgccagc cttggttcag atgataaatc caaagaactt
    14101 aaagatttgt tgacagaaat tactgatatg tcacctagac tatctctttc tgaaaaatct
    14161 ttaaaacgta caccaagttt ctcagtcaat cgtcctggcg aagaaacagg tgtaacattt
    14221 gcaggtattc cattaggtca cgagtttaac tcacttgttt tagcaatttt acaggttagt
    14281 ggtcgtgcac ctaaagaaaa acagtcaatc attgaccaaa ttaaaaaatt agaaggttca
    14341 ttccattttg aaacattcat tagtttaacg tgtcaaaaat gtcctgatgt cgttcaagca
    14401 cttaacttaa tgagtgtgat caaccctaac atcacgcatt ctatgattga tggtgcagtg
    14461 ttccgtgaag aatctgaaaa catcatggca gtccctgctg tctttttaaa tggcgaagaa
    14521 tttggcaatg gtcgtatgac aatccaagat attctttcga aactaggcag tacggcagat
    14581 gcatctgagt ttgaaaataa agaaccttat gatgtcttaa tcgttggtgg tggtcctgct
    14641 agtggtagtg cagcgattta cacagcacgt aaaggtttac gtactggtat agttgctgat
    14701 cgtatcggtg gccaagttaa tgatactgct ggtattgaga acttcattac tgttaaagaa
    14761 acaactggtt ctgaattttc ttctaactta gcagcgcaca ttgatcaata tgacattgat
    14821 gcaatgacag gtatacgtgc tacagatatc gaaaagactg acgaagcaat taaagttacg
    14881 ttagaaaacg gtgctgtctt agaaagtaaa acagtcatta ttgctactgg tgcaggttgg
    14941 cgtaagctaa acattccagg tgaagagcaa ttgattaata aaggtgttgc attctgccct
    15001 cactgtgacg gacctctatt tgaaaataaa gacgtagcag ttatcggtgg cggtaactct
    15061 ggggttgaag cagcaattga ccttgctggt atcgttaatc atgttacatt attcgaattc
    15121 gctagcgaat taaaagcaga caacgtgtta caagatcgtt tacgttcttt atcaaatgtt
    15181 gatatcaaaa caaatgccaa aactactgaa gttgtcggag aagaccatgt tacaggtata
    15241 cgttacgaag acatgaacac cggcgaagaa catctactta acttagatgg tatctttgtt
    15301 caaattggtt tacttccaaa cacatcatgg ttaaacgatg ctgttgaatt aaacgaacgt
    15361 ggtgaaattg tgattgatcg taacaataat acgaatgttc ctggaatatt tgctgctggc
    15421 gatgtcacag atcagaagaa caaacaaatt atcatttcaa tgggcgctgg tgcaaatgca
    15481 gcattaaatg cctttgacta tattatcaga aactaaagtt tcattcattt cactgacagt
    15541 atcttacatt tgttaagatg ctgttatttt tttatcccga cataaaatca tgttatgata
    15601 aacttaatat atacaacgag atgtgcgata atgcttaaag tatgtacctc tcgcaaacaa
    15661 atcaatatga aaaatgatat tgagaaagaa aagtatgtga acgaaattat tatgctttta
    15721 cgtacgatac ctacgtctaa attagtacaa ccaattatgc agttatttat agataaagct
    15781 atccttaaca acgtccaaaa tgatgcatta ttgatagaaa aagcaactca tgcacgtaat
    15841 catttacgtc tatggtcatt gcaaggtcat cgtgacattc caaactaatt caatgttact
    15901 ctattaaaca tgaatacata ttttttcaat taaagataaa ctaaggagtt atattacatt
    15961 atgaaaaaga gattactact aagtacattt ttagcatcga cattaattct tacaggttgc
    16021 gcgtccgatc aatctgataa cgaagatcat catacaagta ctggcatcca tgcacctaaa
    16081 agcgctaaaa aattagaaac aaaagatatc tttaactcag ataagaaaaa tagtgatatt
    16141 agtgatgcag agatgaagca agctatagaa aagtatttgt ctgtaaatag cgatatactt
    16201 gataacaaat atattatgca acataaacta gataaacaaa ttgacagtca aacaaaagtg
    16261 actgaaaaac aagcagaaac acttagccac ttatctaact tagctgttaa aaacgatttg
    16321 catttcaaga aatttgtcac tgaaaacaat attcctaaag aatataagaa acccgttgaa
    16381 ttaatgatga attacttcaa agctttaaat agtacaatcg ccaatgttga tgaagatatt
    16441 gaaaagctta gctatcaacc gcaaaataaa atcaatgttg tcgacgtgcc aacaaaatat
    16501 gccggtgatg ttaataaaaa gcaacaagat aaaattaaag atttcttaaa atctaaaggt
    16561 atcaagagtg atgttatcga taaataactg aaagccatca ttgcgaaatg taacacatca
    16621 ttacatgagt tttaattcat gacaattctt tatctttaaa agtgactaat caatttagct
    16681 aaagtcatca tttgggcatc aagcagcata ttactgtttg atgccttttt aattgcctta
    16741 aattctctat ctatgccaaa attcccatta gttgacgcat ttgactatat acctaacata
    16801 ttccatactt atattgactc tataaacaaa ggatgatcat gatgaaagtt cgaattactt
    16861 ttattatttt agcaatttta tcgacgattg tttgcttgtt cttagcagca atgcatccaa
    16921 caggaccaaa cacagtaact tttgaacagc catatttatt caccctcaac atcattataa
    16981 tggtattagt cgcattacct tcacttgttt tagctatata tgattatatg agttttagaa
    17041 tttcttctgc tattttacaa tttctagggg ctatctcttg gttcttttta tcattgatat
    17101 tatcgctcac acaatttaca ccttttacat tagcgtcatt tataacttca attattttgt
    17161 tcacaagcac aattatcaca ttagccattg gtggtaagtc tgttgaaaag aatgattccc
    17221 cttaaattcc aaatgaaaaa aaggttctga aggccgctat aaaacacagt ttttcagaac
    17281 ctctatactt ctattcaatg atatatggtt tgcaattttc tacctttaaa tccacagctt
    17341 ctgcccttga aactttgtta aaataaacca tcaaacaacg aatgacaact tgatgtgcaa
    17401 caatgacaat atcatctttt tgtgtatctt cattgacaac atgattcata aaatgttcta
    17461 cgcgttgata tacatcttca taactttctc cttcaggcgc tttttgtgaa aaactatgac
    17521 gaaagtcttt aaagtttgga tcattgaaat atttatcata tttcggattc gcactgattt
    17581 catctttata ttcaccctca aatacgccaa gtgaacgttc tcttaataga ggggtagtcg
    17641 ttgatgcaat gtcatatgga aaaatatgtt caaacgtttg ctgtgttctt aataagtctg
    17701 aaacatatac atgtttaatc tgtttctctt taaaataatc acataaatcg tctgcacttt
    17761 ttgtgccagt atccgttaac ggcacatcta attgtccaca aaaataagat cgaaaatgtt
    17821 tattatcata attcgatttt gattcgccat gtctaactaa ataaatcgtc ataatattac
    17881 tccttacctt atgtatttca tatctaccat aacactttga ctactaattc gatatcaatc
    17941 ttaatattct attctaaaaa aagaattaat tcatatttta aattaatgac tctaaaaata
    18001 gtattgtctt taatcaagtg atgtgttact atatgggtgt agttcttaaa tgactacaca
    18061 gtcttttata ttcatgaaca taatactcct ggatggcgca acccaacgtc atcctttttt
    18121 tatttgtcaa atactcgctt ttttatttcc aaaatttcct catattaatt tattactttt
    18181 taatcctatc ttatctacat tttcaaatat tataatacat attgaccatt ttcacgcaaa
    18241 taaaaaagat gacaactgaa tgccatcttt atttcaattt ccaattgaat ttttttatta
    18301 tttacgcata gctcttaaaa ttaacgttac gattgcaatt aagataattg aaccaattaa
    18361 tgctggcaag atgtaaatac ttcctaattc aggaccccat tgtccgaata gtttgccacc
    18421 tacccatgat ccaataatac ctgcgataat attgcctaaa ataccacctg ggatatcttt
    18481 acccataata gcaccagcag cccatccaat taagccaccg acaattaaca ttccaataaa
    18541 tccaaacata attttcagtc tcctttttct atttattttg cgttattcta agtagtaccc
    18601 cttatttaca attctaaaac aaattcaaat tatttttatc caaatatttt taaaagtagt
    18661 aattgaatat caattttatt caatgtagct atcgttattt aaagtctctg taccgataat
    18721 atcatataca tttacattat tttttctgcc gaattcatag cttgattatt ttatgttata
    18781 ggactagaat atacacatat tattagagca tctttgaatt ttaaatcaag aagcgaggtt
    18841 aatgaacaat gaatatgcat attttatata acttacgaac taaacataat ttagaaattg
    18901 acgaattagc acagcaatta aatgagaaat atggtactaa atatgaagca catcaaattt
    18961 gggaatggga gaatcatcac catgaaccta aatttaaaga tgccatgcat ttagctgact
    19021 tctttgatgc accatatgaa atgtttttag aaagtaaggt taaagaatat cagaaacatt
    19081 tagaagaagt cgatattcgc atggataaat agatgcaaat aaaccctcac aacacgtttg
    19141 gcatatatcc tttcaaatct atactggata tattacatta cgttgtgagg gtattttatt
    19201 aattaatatg aattaagaca ttttacaagc gttaatgcaa cgaatctttt tagtgatctt
    19261 gctcactctt taatacttta ccgttcttag catcaacagt aacttcttgt tttttattac
    19321 cttttttcaa atcgatattg taaacaagtt tgccatcatc tttttcaagt gaccattctt
    19381 taatatcacc atcaaattct ttttgtcctt ctttaatggc ttttttgtaa tctatagcat
    19441 cgctatattt aaagttatca ttttcattca ttgtatcttc tttttcagtc tttttgttaa
    19501 tcactttttt atttttatca gcaacaagta cttctgactc ttcaccagat ttttgttgcg
    19561 tcactttata agcccattca ccattagaat tttcaaatga aattcctttc aacttttggc
    19621 ctttgtaagt ttcttcagct tttttcacag catcttctgg gcttgtttta acatctttta
    19681 aagcaataac atcttttgtt gtattagtgt cttgattagt atttgactct gttgattttg
    19741 tttcatcttt tggagtatca ttgccacatg cagtaagcac caccgctgac attgataaca
    19801 ctgctaatga ttttaatttc ataatatcac tctctcttcc tatttttgaa actcataaca
    19861 aaagcttata tgctatatag attgtattac cccttgtttt taattttatt cataattatt
    19921 acaaatattt ttaaattaat cgtcatgcgt tactttcgtt cgtactcctt ttataaatga
    19981 accatgtaat ataagcatgc tattatcgac tcagtttgtc taatgctttc tttggtactt
    20041 cttccttttc aacttcttca aaagtttcta catgatgacc tttatgtgta attttcaagt
    20101 atctatgcgg tttaacatca aatgtagcag tatacattaa ttgcgtctct ttcccttctt
    20161 tattaaaaac actttgacta taacttcgga gttgatcatc cattctagta gacacctctg
    20221 tcgttttaac ataagaatca tccttcttta ctaatgggtt aaattgatct gttataccat
    20281 gggagtctat tgtttttaaa atgaataaag cagcataagc gccaatgata gtaagtacaa
    20341 gatatgttat tgtttttaaa atcgttttca aaagatcacc tctgacaaaa tataaaatag
    20401 gatgtatctg aaaacaactt acataattgt aattaaagtg taagtattag gagaaaatga
    20461 tggtatttga atctgaaaga tacgaatcgt ttttaaacta agttatttta tagagacttt
    20521 attatgaaat tgaaagaagt taattttaag gttgtcgaat gtaaaaacat attgattaac
    20581 cataaaagag aaattatttt tgtagatttc ggcataaacg tcattaaaga attttctata
    20641 tgatggtgct aatcataaca aagaaagcta ggcaccttta gatacctaac tttcaaagta
    20701 ttagcgataa aacactatat atactaattt agcttaattt caaacttgtt caatgtcatt
    20761 aacacttttc acaagtttga aagttacatt ttttatatcg gctgtattaa tttttacatt
    20821 ttccctatct ttttgtagct ctttatgcga ctctaatgta tatttaccac catcttttgt
    20881 atttatgacg atgttacctt taggcaaatg ttctcccata tacaagccat aggaaatatg
    20941 agcaaatctt atgatatgat ccaattcttt taaagtgaca acttctttat taagagtaat
    21001 attcctctta ggtgtataca ctctgccagc aactgtgtat gttccttcta aatgtatacg
    21061 aacttggttt tcatttaaag gtctctctga ataaacccaa ttcctagatt tattagattt
    21121 tccatttatt gtatccttgc tgtatctatc gtaaagtttt ttaaccaaag ctttttctga
    21181 attatttggt acttcttcaa ctttttgtaa ttgtttatct tgtacacttg aggaattttg
    21241 tgttgacgcg tctgcttttg gatatattcc aaatgttccc ccatagatga tacctagcga
    21301 tagaatagat tttaatacga ttgaattttt tgagtcatat tttttgaaca tatttaatta
    21361 cctccttgat gtaaagcttt atttgctaca attataaaaa taatagacgt gttcatgaat
    21421 taaattcatc ttaactcttg attaacttta atttgctacc actctgaatt taataactat
    21481 aaatcgtcta cacataattg gacaaaatct aagagaataa aatttgttaa tttaaaatag
    21541 caagcaattc aaagttatat gtgtaataga taaaatagat atccctatag tgatgcgtta
    21601 ctagctaaac ataataacac attagaagat aatgaagtta aggagttact ggattgtttc
    21661 gactatgtaa ttaagtataa aaatatccaa cgacaaaacg taattataaa atggtaaaag
    21721 ctatggtaca gtttcaaatt gctaatgaca tgcgtatcgg tgagctactt gcaataaaga
    21781 gagtaaatat aaactatgaa gataaaacgc tagatatcga cggtaaagtt aattggataa
    21841 ctgaaaaaag acgggagcat tcggagtaaa ggagacaact gaaagaagta atagctataa
    21901 ggccacaggg ctcactaccc aaagcatcga cttactaaga acacttatgc ttggaatgat
    21961 aagtttattg atagagagta catattcaca aatacgtctg gtagccctat cgactcgaac
    22021 aaaattagcc acattattaa aggggggcgc tgatattagt tctattaaga aacctataac
    22081 gacgcataca ttacatcatt cgcatatatc tacacttgct caattaggaa ttaacttaaa
    22141 agcaatgcaa gagcatgtag gtcattcaga ttataaaaaa aatctagaga tatacacaca
    22201 tgttactaat cagatggcga aagatatgat gaataaattt gaacgattgg ggagttaaaa
    22261 ttggaaaaag atgatacact agcagaaatt aagcctatgc tcaattttga tgagcaaata
    22321 gcgaaattaa aacagatgaa tatatttttt aatattattg acaccgaaaa agcaaatgaa
    22381 attcttagaa aaaataatta cttcttcaaa ctagcttatt tccgaaaaaa tttcgaaaaa
    22441 aagaatggcg gctatttcat agaatttgct tatttatcag atttagcaac tatagatatg
    22501 aaattaagat acacaatgtt gcatttaact ttagatattg aacatagttt aaagtatcta
    22561 gtcttaaaac taataacaga aaataaccaa gaagatggtt ataaaataat agatgagttc
    22621 ttatgtattg ataaatcata tagcaattca aattttgaca caaattcaag aacaccagaa
    22681 gaagttatgg aaaccaaaat caaaaataaa aacgaaatat tcaagcatat gaataaacga
    22741 ggacaactac ccgagaagtt gaataaatac tatcaaaatc cacccgcatg ggtttgcatt
    22801 gaattcatgc aactaggtca attcgtttcg tttctcaact tctattacaa gaagtacaat
    22861 gacgaagaat tgagagttgc taatatttta atgcctttag ttaaaaatat aagaaacaaa
    22921 tcagctcata accaacccat catagcaaat ctaaattatg acagtagatt acctcaatat
    22981 ttatttgaaa aagggaataa tataggcata tctagaaaca tgttcggaat aaaaaatttc
    23041 atagatactt tcgctacgct agaattacat aatcaagttt gtagtaatgc aattatccaa
    23101 gcaagatatc acgatttgga ccaacttcaa aagcgatata aaagaaacga aagctattat
    23161 aataatgcat tagctatcaa aagatttttt atagctttag ataaaattat tgacttcaac
    23221 agaccaaaag tataaactat ctagtgagga aagagactta taggtctcgc gagttatttt
    23281 aattcgtatg caagaaaaag aagagctatg cattttattt aaaatgcgta gttctttttt
    23341 tatgcatcta aattcatatt atttttgcaa tataaacata tctttgtgca aattccgaac
    23401 acaaaacatt cacatcatcc ttttttgccc tttttctata ccccaaaaca caaaaagccc
    23461 cgtaagccta tgcctacggg gtttgacaat aaattatata ttattgttct tctttaacat
    23521 atggtaataa tgccatatga cgagaacgtt tgatagctgt agtcaacata cgttgatatt
    23581 tagctgaagt accagttaca cgacgtggta aaattttacc gcgttctgag ataaaacgtt
    23641 ttaataattc agtgtctttg tagtcgatat gtgtaatacc atttgctgtg aaatagcata
    23701 cttttttacg acgacgtccg cctcttcttg gtccacctgc catgattgtg tgcctccttt
    23761 gataattttt tcgctttaat ttcgttaatt tttattagaa tggtaagtca tcatcactta
    23821 tatcaatcgg tccgtttgca tttgcaaatg gattatcaga ttgtttcgtg tttgatgaat
    23881 tattgtacga attgttttgt cctgattgtt gaccaccgaa tccttgaccg taatcttgga
    23941 attcattttg ttgacgttgg ccaccatttt gttgcgcatt tttaggttca aggaattgaa
    24001 cgctatcaca cacaacttca gtaacaaaca cacgacgacc ttcttgattt tcataattac
    24061 gggattgtaa gcgaccatct acaccagcta aactaccttt agataaatag ttatttacat
    24121 tatctgcttg tcttctaaaa acaacacagt taataaaatc tgcttcgcgc tccccttgag
    24181 cattcgtgaa cgtacgattt actgcaagag tgaatgtcgc tacactcaca cctgagggag
    24241 tggttctgta ttccggatct ttcgttaaac gacctactaa tacaactcta tttagcattt
    24301 aaacgccccc tctaattatt acttgtcttc gtcttcacga ataaccatgt aacgaatgat
    24361 atcgtcactg attttagcta gacgttggaa ttcgtcagta gctttgttgt tatcagattt
    24421 aacacgtacg atgttgtaga agccatcttt gaaatcattg atttcataag ctaggcgacg
    24481 tttaccccag tcttttgctt ctaaaacttc tgcaccttca gtagctaaga taccgttgaa
    24541 acgttcaact aacgcttttt tagcatcttc ctcaatgttt gggcgtacga tgtacataac
    24601 ttcatatgtt ctcattttat atttgcacct ccttgtggtc tatacggctt atcaatctta
    24661 aaacagataa gcaaggaata attttcatta ctcacaataa agaattatat catgcgccat
    24721 tactttttac aataataatt caaactactc ttcatatcat ttttgatatt aattcatttg
    24781 aaactttcta tgatattttt aaaaatacac ttcacaaaag cgaacatatg ttctataata
    24841 gttgtgaggt ggtaaggaat gaatttaaat attgattggt ctaaagattt tcaagaattc
    24901 caagagatac ttaatagtgg tattcatcct gaatggcttt attgtgcaaa ggctaatctt
    24961 gttttagagc ctgcttatac tggcgaaggc aaacaatttt tcagcactca agatattatt
    25021 aacgcgagta aaattattcc attcttttaa taaattatac gcataattaa aagaggcgaa
    25081 caattgattc cgcctcttct acatatatac tatttaatta acttcactct atcgtttata
    25141 cattaaatct gaaatgaacg atatcgccat cttgcataat atattcttta ccttctaatc
    25201 gttgtctgcc cgcttcttta gcgccacttt cgccaccata ttgtacatag tcgtcataac
    25261 ttgttacttc ggcacggata aatccacgtt caaaatcagt atgaatgata ccagcacatt
    25321 gaggtgcagt cataccttgt ttaaatgtcc aagcacgtac ttcttgcaca ccagcagtaa
    25381 aatatgttga taatcctaat aattcataag ttgttctaat taatcgatct aatcctggtt
    25441 cttcgatacc taaatcttct aagaacattt ctttatcttc atcatctaat gtagcaattt
    25501 cttcttcaat ttttgcacta ataacaatca cttcagagtc ttcttgcgct gcatattcac
    25561 gaatcgcttt tactttatca ttatcatcat caccaatttc atcttcacca acattagcga
    25621 tataaagcat ttttttagaa gtcagtaatt gcgcttgatt cacccatttt tgatcttctt
    25681 cattaaagtc aatactacga gcgggtttac cattttctaa agcttcttta atagttgtta
    25741 aaatacgtac ttccatttca gcagtcttat ctttttgacg tgctaatttt tcaattctag
    25801 gcaaacgttt ctcaacagat tctaagtccg ctagtactaa ttccatatta ataacttcaa
    25861 tatcatcaat agggtctact cgaccagcaa catgagttac gttatcatca tcaaatgcac
    25921 gaacgacctg acaaatcgca tctacttctc taatatgtga taagaattta ttacctaacc
    25981 cttctccctt tgaagcacct ttcacaatac cagcgatatc tgtaaattca aatgtagtcg
    26041 gcaatgtctt tttaggttga accatttctt ctaatttaag taatctagca tctggcactt
    26101 ctactatccc tacattagga tcaatcgtag cgaatggata gttcgctgct aaagcacctg
    26161 cttttgttat tgcattaaat aatgttgatt taccaacgtt tggcaatcca acgatacctg
    26221 ctgttaaagc cattaatcat tctcctatct ttgtgtatca tcatgagatt cgatgatttt
    26281 tttaagtttt ttatcaaacg tttgacgtgg aatcataata cttctttgac aattttcaca
    26341 tttaattctt atgtctgcac ccattctaat aatcttaaaa cggtttgttc cacacgcatg
    26401 ttgttttttc atttctacta tatcatttat tccatatttt gacgccatta aatatgacct
    26461 ccatgtatta taaactacta aacaccgttt tcactatgat tataagcagt cataattggt
    26521 tgaggtgttt taataccttc ttgtaaaaac attttttgta cttctttgcg aataattcga
    26581 gctccagcaa atccttcacc tggaattgtt tctgcagata ttctcaatat tactcttgta
    26641 tcttcaatag catcaatacc aataacaacc ggatcactaa caaataagta atatttacta
    26701 cgtaaagaag taaatagttt gtttagtttt ttatcaacat tatcaatgtt ttcttcgaca
    26761 gacactggaa ttttaacgat agctgtacca tttgtaattg agtagttcgt tatttcaccc
    26821 atactactat ttggtaaaat tgttaattct cctgaaattg tattgattcg tgttgaacga
    26881 agtccaattg atttaactgt tccctctgct acagttgtac caccgttatt tatcttaaca
    26941 taatcaccta catcaaattg gctttcaaat ataataaaga atcctgtaat tacgtcttta
    27001 actatagttt gagcaccaaa acctactgct aagcctacga caccagcact ggcaattacc
    27061 ccttcaacac taatgccaaa tttacttaaa atcgtcgtaa taactataaa ccaaacgata
    27121 tacttcacta cattttgaac aagagatatt aaagttttag agcgcttttt gttactcttt
    27181 ttacttttat tttgaatctt aaatccctgt tcaatcattt tattcagtat tttaataact
    27241 atgagggcta cgataatata aataacaatc atagcgattt tagttgcaat gttttcatat
    27301 gtttctattt ttgttaatgg ctcaaataga gatgaaataa tattcatgac ttgattcatg
    27361 ataagcgtta ctcccttcta tatgatatat caatattgta ttcatatttt tatatacttc
    27421 caacctacga aagtctcaat atatctagaa tcaaatgttc actgattaca atttatttac
    27481 acttgcacaa agagttatta tataaaattg tgtaactatt taccatacct acgatttaat
    27541 tgttcaatta ttctcacaaa atcttcttgt gaatcaaact caaatgagat tttaccaacc
    27601 gattttttta ttgatatatc tactttggta ccatactgtt ctcgcaactg tcgttcttgc
    27661 tgctttataa atttaggctt agttatatct actttgtctg tttccgactt tgacgaaaca
    27721 ttttttaatt cattaacatg gttttctaaa tatctgacac tccacttttc tttaacaacc
    27781 cgtttcgcta acctaagcat ttgttgttca tctttaattg ccaataacgt tcgtccatgt
    27841 gcacttgtca gtcgcccatc ttttaccatg tcagcaatct ttttcggcaa atgtaataac
    27901 ctcaacatat tcgctatata cgggcgcgac ttactcaatc gtttcgctac ttcttgttgt
    27961 gtaattttca aatctgtcat caaacgttga taactttcag cttcttcaat cgcatttaag
    28021 tcttctcgtt gtaaattttc gatgaccgcc agttccatca tatcttcatc tgttaaatct
    28081 ttgataatcg ctgatacgta ttttagacca gcaattttcg aagctctaaa ccttctttca
    28141 ccaactacaa tgtaataacc ttgaactgtt tttctaagca caattggttg caaaattcca
    28201 tattgcttaa ttgaatctgc caaatcattt aaatgatttt catcgaaagt ttttcggggc
    28261 tgatacggat tcggtttgat atccgatata tctatttgct taatttgttc atcttttgtt
    28321 ttagtaatac gttgatcttc actttttgac aattcactca catcactttt tatttgataa
    28381 caacagttaa atatatttta tgtattctaa atgagtcaga caaccattaa ccacacttta
    28441 tttaaaaaat aattcatttt tcaacttcaa gataccaatt acaatttcac aaacaaatta
    28501 acttttaaat caaaattttc tgaataattg ttgacaaatc tttttttact ctgtaagcta
    28561 attaatatct tataacagtt taatgaaacg taaacacaat aaagaggaaa gtaaaacaca
    28621 ccctgcttat acagagagtc tttagtagct gagagaagat tttgaaagcg tgtttgaaaa
    28681 tggccttgga gtgttgatgc caatatgagg tgtctacggg ttcgcccgtt atagcgatac
    28741 agtattaaca ttgatgttaa atggcgtact ggattcttta cgcacgattt tttgttaata
    28801 agtatgggat agcacattac tatatcctta cttactgact ttaattgtga taattgttca
    28861 gtaagcatat ttacttttaa tgcgtactga ataaggttat ttcagcgatg gaataacaaa
    28921 taaaggtggt accgcgaaac ataagctttc gtccttttta tccgattcat tcgggtacga
    28981 aggacggaag ctttttttat tttttcttat aggagggtct taatatgaag gatacacagt
    29041 tagcccaaat cacattaacc gatgattcaa ccggtgctat agcgaatcca atccatttat
    29101 ctactgccta caagcatcca aaactaggac aatcgacagg ttttgattat acacgtacta
    29161 aaaatcctac acgctcaaca tttgaaacct gttttgccaa acttgagcat ggtattgcat
    29221 cattcgctac atcaagtgga atgtcagcca ttcaattaat atgtaatcta tttaaacctc
    29281 atgatgaaat tttagtttca ttcgatttat atggtggcac atttagatta tttgaatttt
    29341 acgagcaaca atacgatatc aaatttaagt acgttgattt tacagattat gaacaagttg
    29401 aaaaagaaat cactgataaa acagttgcat tattcattga accaatatct aacccacaaa
    29461 tgattgctat tgatgtaaag ccatactatc aactttgtaa agctaaaggc ttattgtcaa
    29521 ttatcgacaa tactttttta acaccttatc tttcaacacc actagcagaa ggtgctgata
    29581 tagtcttaca ttcagccacg aaatatattg gcggacataa cgatgtacta gcaggtgtcg
    29641 taaccgtcaa agatgaatca ctcgcgcaac agttgtttga ttttcacaac atgactggcg
    29701 caacactttc accaatagat agttatttgt tgttacgtgg acttaaaact ttgcatttac
    29761 gcattgagcg tgcgcaatca aacgctagaa aacttgctaa aaaatgtcag tcacttcaag
    29821 caattgacga agtactatat agcgggcaaa ctggcatgct tagtttaaga cttaacaagg
    29881 cctatagcgt cgctaaatta ttagaaaatt tagacatttg catttttgca gaaagtttag
    29941 gaggtactga aacattagtg accttccctt acacccaaac acatgttgat atgccagatg
    30001 ctgaaaaaga taaacgtggc attgatgagt atttaatccg cttatcacta ggtgttgaaa
    30061 attatgaaga catcgaacgc gatatcatcc aagcattaga taaagctcag attggagaga
    30121 ttgtatgaca ctttcaaaag agacagaagt gatattcgat tggcgtagag gtgtggaata
    30181 tcattcagct aacccaccac tctatgattc ttcaacattc catcaaacaa gtcttggtgg
    30241 cgatgttaaa tatgattatg cacgaagtgg caaccctaac cgtgaacttt tagaagagaa
    30301 gttagcacga ttagaacagg gaaaattcgc ttttgctttt gcatcaggta ttgctgctat
    30361 ttcagcagta cttttgactt tcaaatctgg tgatcatgtc atcttacccg atgatgtata
    30421 tggcggtact tttcgcctca ctgagcaaat tttgaatcga tttaatattg aatttacaac
    30481 cgtagatact acaaagctcg aacaaatcga gggtgccatt caatcaaaca caaaattaat
    30541 ttatatcgaa acaccatcga atccctgttt taaaattact gatatcaaag ctgtttctaa
    30601 aatagccgaa aagcatgaac tactggtagc tgtggacaat acatttatga caccgttagg
    30661 tcaatcacct ttattacttg gcgctgatat tgtcattcat agtgctacaa aatttttaag
    30721 tggacatagc gatttaattg ctggtgctgt cataactaat aatgaggcaa ttagtgacgc
    30781 tctttattta atacaaaatg gtacaggcaa tatgttatct gctcaggata gctggacact
    30841 tgctaaacat ttaaaaactt ttccaatcag atttaaacaa tctgtcgaaa acgctcaaaa
    30901 aatcgtgtca tttttaataa agcaagatga aatttcagaa gtttattatc cgggactcac
    30961 tactgctcat ttagaacaag ctaaaaatgg cggtgccgtt attggctttc gtttagctga
    31021 tgagtctaaa gcacaacaat ttgtcgatgc actgacatta ccactcgttt cagtgagtct
    31081 gggcggtgtt gaaacgatcc tttcacatcc agcaacaatg tctcacgctg cactacctga
    31141 agaagtgaga caagaacgtg gtatcacttt cggtctattc cgattaagtg ttggtctcga
    31201 agatcctgat gaactcattg cagacatcaa atacgcatta aaggaggcat tcaatgagtc
    31261 aattcctcac acaattgaaa gataatgttt tagtagctga tggcgctata ggaaccattt
    31321 tatactctga aggattagac acctgtccag aagcatataa tcttagccat ccagataaag
    31381 ttgaacgcat ccatcgttca tatattgaag ccggtgctga tgtcattcaa accaatactt
    31441 atggtgcaaa ttttgaaaag ttaaaacgat tcggtcttga agataaagtt aaagcaatac
    31501 atcaagccgc cgttcgcatc gcaaaaaaag cagcaaataa agatacgtat atattaggca
    31561 cagttggtgg gtttagaggt atcaaacaag aggatatcag cttacaaact attctttatc
    31621 atactgaaat tcaaatagac accttaattg aagaaggcgt tgacgcgcta cttttcgaaa
    31681 cgtattacga cctagaagag ttaacaaatg tcatttcacg aacgagaaag aaatacgaca
    31741 ttccaatcat tgctcaatta accgcttcaa acacaaatta cttagttaat ggtcaggcaa
    31801 tcaatgaagg attaaaacaa ctcgttcaat gtggtgcaaa catcgtggga ctcaattgtc
    31861 atcatggccc gcaccatatg caagagtctt tcacacatat tgaattacca gagcacgcat
    31921 tcttatcttg ttatccaaat gccagcttat tagatattga aaatagtgaa tttaagtata
    31981 gtgacaatgc acaatatttc ggtcaagttg ctcaaaatct aattcgcgaa ggtgttcgtt
    32041 taattggtgg ttgctgtggt acaacgccag agcacatcaa atttattaaa gaatctattc
    32101 agacacttaa acctgttaat gacaaaaaag tgattccgat accaacgaaa gcacttttca
    32161 atccatctca aaataaagtt agacaatcat taacatctaa ggttcaagaa cgtccaaccg
    32221 ttattatcga attggataca ccgaaacatt tagacacgga tagatttttt gaaaatatcg
    32281 ctaaacttga taaagctaat gtagatgcgg taacactcgc agataattca ttggcaactg
    32341 tcagaattag caatattgct gctgctagct taattaagca atattacaat attgaaccac
    32401 tcgtacatat tacatgtcga gaccgaaact taatcggctt gcagtcccat ttacttggat
    32461 tatcgctcat tggcgttaac gaaatattag ccataactgg tgatccttca aaagttggtc
    32521 acttaccagg tgcaaccaat gtctatgatg ttaattctaa aggattaact gaactcgctc
    32581 taagatttaa tcaaggtatt aacactgacg gtgatgcgct gaagaaacgt acacacttca
    32641 acatcgctgg cgcctttaac cctaatgttc gtaaattata tggtgccgtc aaaagattag
    32701 agaaaaagat agaaagcgga atgtcttatt ttataacaca acccgtgtac agcaaagaga
    32761 aaatcattga aatttaccat gccactaagc acttgaacaa accatttttc ataggcatta
    32821 tgcctatcgc aagttacaaa aacgcactct ttttgcataa tgaagtgcca ggaatcaaga
    32881 tgtcagatga aattttacaa caatttgaag cagttaaaga tgataaagcc aaaacacgag
    32941 aactaagtct taagctttca aaggatttaa tcgatactgt tcatgaatat tttaatggtt
    33001 tatacattat cacaccgttt caaaatgtcg aagattcatt agaacttgca gcatactcaa
    33061 aatctattac tgctcacaag gaggcaatat tatgacaaca attaaaacat caaacttagg
    33121 attcccaaga ttaggtagaa aaagagaatg gaaaaaagcc atcgaaagtt attgggcaaa
    33181 gaagatttct aaagaagaat tagatcaaac attaactgat ctacataaag aaaatttatt
    33241 attacaaaag tactatcact tagatagcat cccagttggt gatttctctt tatatgacca
    33301 tatattagat acgtcattat tattcaacat catccctgaa cgtttccaag gaagaactat
    33361 tgatgatgat ttattatttg atattgctcg cggtaataaa gaccatgttg caagtgcact
    33421 tataaaatgg tttaacacta actatcatta catcgtacct gaatgggaca atgttgagcc
    33481 taaagtgagt cgcaatgtat tattagatcg ctttaaatat gcccaatctt taaacgttaa
    33541 cgctcaccct gtcattgttg gtccgattac ttttgttaaa ttatcaaaag gcggtcacca
    33601 aacttttgaa gaaaaagtta aaacgttatt accactttat aaagaagtgt ttgaatcatt
    33661 aattgatgca ggtgctgagt acattcaagt tgatgagcca atcttagtta cagacgacag
    33721 cgaaagctat gaaaatatta cacgtgaagc ttatgactat ttcgaaaaag ctggtgttgc
    33781 taaaaaatta gtcattcaaa catactttga acgtgcacat cttaaattct taagttcttt
    33841 acctgttggt ggtataggtt tagactttgt ccatgataat ggctataacc ttaaacaaat
    33901 tgaagctgga gattttgata aatcaaaaac attatacgct ggaattattg atggtcgtaa
    33961 tgtatgggca agtgacattg aagctaaaaa agtcttaatc gataaattgt tagcacacac
    34021 taatgaactt gtcattcaac catcatcttc attattacat gttccagtat ctttagatga
    34081 tgaaacatta gatacaagtg ttggcgaagg cttaagcttt gcaactgaaa aattagacga
    34141 attagatgca ttgcgtcgcc tatttaatca aaacgatagt gttaaatatg ataaattaaa
    34201 agcacgttat gagcgtttcc aaaatcaatc attcaaaaac ttagattatg atttcgaaag
    34261 cgtgcgtact tcaagacaat caccattcgc gcaacgtatt gaacaacaac aaaaacgttt
    34321 gaacttaccg gatttaccaa caacaactat tggatcattc ccacaaagcc gagaagttcg
    34381 aaaataccgt gcagattgga agaacaaacg cattacagac gaagcatatg aaacattctt
    34441 aaaaaatgaa attgctcgat ggattaaaat tcaagaagac attggcttag atgtattagt
    34501 tcacggtgaa tttgaacgta atgacatggt tgaattcttc ggagaaaaat tacaaggttt
    34561 cttagtaact aaattcggtt gggtgcaatc atatggttca cgtgccgtaa aaccaccaat
    34621 catttatggt gatgtaaaat ggacagcgcc tttaactgtt gatgaaacag tttatgcaca
    34681 aagcttaaca gataaaccag ttaaaggtat gttaactgga cctgtaacaa ttctaaactg
    34741 gtcatttgaa cgtgttgatt taccacgtaa agtcgttcaa gatcaaattg ctttagcaat
    34801 caacgaagaa gtattagcac ttgaagctgc aggaatcaaa gttatccaag ttgacgaacc
    34861 tgcattacgt gaaggcttac cattacgctc tgaatatcac gaacaatatc ttaaagatgc
    34921 tgttttatca tttaaacttg caacgtcttc agttcgtgat gaaactcaaa tccatacaca
    34981 tatgtgttat tctcaattcg gtcaaatcat tcatgctatt catgacttag atgctgatgt
    35041 tatttcaatt gaaacatctc gtagccatgg tgatttaatt aaagactttg aagatattaa
    35101 ttatgattta ggtattggtc taggtgtata tgatattcat agtccacgta ttccaacaaa
    35161 agaagaaatt actacagcaa tcaatcgttc actacaacaa atcgaccgct cattattctg
    35221 ggtaaaccct gactgtggtt taaaaacgcg aaaagaagaa gaagttaaag atgcattgac
    35281 tgtgcttgtg aatgctgtta aagctaaacg ccaagaataa tcgcagcatt tatcatataa
    35341 taaactaaaa acgggtgatt ttatgacaca atatccttta tggcaacaat taaatacttt
    35401 aaaacaagca acttgggttg atttaacgca tacatttgat ccggaaatac cacgttttag
    35461 tgagtttgaa aaaggcgaag tttcaacttt attcacggtt aaagatcatg gtttctatgt
    35521 gcaacgttgg aatattgtaa cgcaatatgg tactcacatt gatgcaccaa ttcactttgt
    35581 cgagaataaa cgttatttag aagacattga tttaaaagaa ttggtattgc cgttaattgt
    35641 attagacttt tcaacagaag tagcgaacaa caatgatttc atcgtaacac gtgcgcatat
    35701 agaagcatgg gaaaaagagc atggtactat tgaacctggc acttttgttg cacttcgtac
    35761 cgattggtca aaacgttggc ctaatattga aaagtttgaa aacaaagatg cgaacggaca
    35821 acaacatgca ccgggttggg gattagatgc tttgaagtac ttaattgaag aacgtcgtgt
    35881 tgaagctgtt ggtcatgaaa cattcgatac cgatgcatct gtggacgttg taaaaaatgg
    35941 tgatttagtt ggcgaacgtt atattcttgg tcaggataaa taccaagtcg agttattaac
    36001 taatttagat caattaccaa ctcgtggcgc catcatttac gctatcagcc ctaaaccaaa
    36061 agatgcacct gggttccctg ttagagcatt tgcaattaaa ccatcaaatg actaatatta
    36121 cccaataaat ccataaacaa ataaaaacag gttatgccaa catgtaatta atactgttgt
    36181 gataacctgt tttcttatta tttatacttt gatacaactg cagcgatagc ttgaccgcca
    36241 ccaatacaca atgatgttaa accagtttca acttcatcat tcagttgatg caataatgtc
    36301 actaatactc tagcaccaga tgcaccaata ggatgtccta atgcaatagc gccaccttta
    36361 acattcacct tttcaggagg taattttaat tcacgatcaa cagctaatga ttgtgctgca
    36421 aatgcttcat ttaattcaaa tacatcaata tcgcttaatt cttttttact acgtttcaaa
    36481 gccttttcta cagcgccaac tggtgcaata cccataatag aaggatctac accatgactt
    36541 ccaaagccat caagcactgc caatggttcg atatttaatt ctttagcttt gtcttctgac
    36601 atgactaaca tcatcgcagc accatcattg attcctgatg cattacctgc tgtaactgta
    36661 ccgtcttttt tgaaagctgg tcttaatcga cttaattttt cgactgatac attttcacgt
    36721 acaccttcat ccttagtgac tacgattggt tcacctttac gttgaggaat cgatactgga
    36781 actatttcac tatcaaattc accattttgc tgtgcacgta ctgctttttg ttgtgagttt
    36841 acagcaaatg tatcttgttc ttctcttgaa ataccatatt gctctactaa attttcagca
    36901 gtaataccca tatgatattg attaaataca tctgttaaac catcatatac catgctatca
    36961 accattgatt gatgtcccat tttaaaacca aagcgactgt tgttgacaag cattggtgat
    37021 tgagacatat tctccatacc gccagctagc acgatgtcat tttcaccagt cacaatagat
    37081 tgatatgcta attgaatcga ctttaaccca gaaccacata ctttattcac cgtaaatgca
    37141 ggtactgttt ctggcaagcc acctttcata gcagcaattc gtgctggatt ttgtccttgt
    37201 cctgcttgta gtacgttacc gatgataact tcatcaatct cacttggatt caaacccgtc
    37261 tctttaataa tatgttctat taaagtcgca cctaaatcat aggctggcac gtctttaaac
    37321 gcacctccaa aaacgccaat aggtgtcctg tatgctgctg ctaatacgac tctcgtcatg
    37381 tcatattcat ctccttatga tatatacaat tttattacta atacgtctaa tgaaccaata
    37441 cgccactaac atccaattta tgaaaccaat tgctacgtat gttacatttt cattcttgtt
    37501 atgcatttaa caaatcatat tatacaatga tgtaatttgt ttgggcaatc ggaaacgtaa
    37561 acggtttcac tttaacattt caccctctta aatatgctct atcatcctca tctatagtac
    37621 aaaattatgc tttgatttac cacaaattat cattaatcat ttcgcgtaat gactctaatt
    37681 aaattctatc tatcattgca tcgctattca cagctggttt caccatcaat ttctcatcaa
    37741 ttttttatta ccatgacgtc tccagtcaaa tattaaatcg ctgatgattg ttaattccgc
    37801 aattaccttg cagtcacctc tccttccact tcatcgccaa atctgcccct attcactaga
    37861 aaaaataaat agtatactta gtaaattaat tacacatctt aaaggagtta aaattttatg
    37921 gttaatgcat taacaccaaa acatttagct gaaaagcgtg caggattcca tgagttgttc
    37981 tttgatttaa ttttcgtcta tgccattcaa aaaattgcac atgtgatttt aacaacacaa
    38041 aacggttcca tttcagctga tttatttttc aaatacatcg tcatgagttt gttcttatgg
    38101 ttaatgtggt ctcatcaaac attttttaca aatcgatttg gtcaagttac attcaaagac
    38161 gtctcgttta tgatgtttaa tatgttcatc atggtcttcc tatctaacag cctatatccg
    38221 gactttgaaa agacattctt cccattcttc ttatgcgtgg caattatgta tttaagtatc
    38281 ggattacagt atttattaca tatacgtaca gggttagatt atggcgataa gcgtacatgt
    38341 caagcttttg cgagcgtagc ctttgtgatt tcatttttat catttctatc attagtacta
    38401 ccacaaagta tccattacat ccctggattt ttaggcgtat tcattgcagc gacaggactt
    38461 attccatttc agaaatatct tgtattatca cctgttaata tgatgcattt agtagaacgt
    38521 ttttcgttac taacaattat tatctttggc gaggtactgg ttggtttagc ttcgtcatca
    38581 tttagcattg atcattttag ttacatttat atattccaat ttatgatttt aattagtctt
    38641 ttcggtgtat attggatcat tactgaaaat tatattaatc ataaattaac atctatcggt
    38701 ttccgtttat cgtatacgca tctattaatc aatatcgctt taggtgtgat taatgctgca
    38761 attgttttta gtaataacaa taaactaaat gacttgtttg aaattaatat gatgtatatc
    38821 agtgttttaa tattctatat tggattatgg ttaatcacgc cttatttcca taatgaactg
    38881 acaaacgcga agtatatttc atattcacta ggtatactcg ttatgtctta tattgtcagc
    38941 cttttattta aaggtcacga ccatgtaatg attatctcag tatcagttgc aaccttctgc
    39001 atcatgctga tttattttaa aaatcaacgt ctacgtaata aacatgaata aatagtattt
    39061 acctcggtca aagtattgga actgaatcca aaagtgtcga acttcacatt atacttttgt
    39121 gacaaacttc agttatgcta catgatcgag gtttttaaat atttccgagg aaatcgttag
    39181 cctatacaat catcaaatga tacttataaa taacatatat cctctaatcc ccactatcta
    39241 aaaggaaata attcccacac aacataacca cctcaaaaca aataaatcaa tatagatatg
    39301 tcacctatac ttatccccca catttcattc cacaccaaaa agaacagatg acaatcgtaa
    39361 agatttatca cctgctctaa atattatttc aaatactatc atcaccaaaa tcgacaatta
    39421 tagttcttgt ttgttaaaaa ttttaataga caatatataa gaaacgctcg ttataactat
    39481 accaatgacc gcatatgcta tgacaactag ccatccttca gtgctaattt tttcaaattc
    39541 taatgcagat gcaccactaa ccataccata tataaagaaa aagaccacga aagaaataag
    39601 tagtagtata gatgcacata tcataatgac attggagttt tcagcgccaa atttaaatgt
    39661 caatgggaaa atgatagagt atgcccctat gataccaaaa ctcattaaac ctgacataat
    39721 accaatcatc acactttggg tcacaatcgt agtcacaact aatccaatca ttaaacttgc
    39781 accgaataag attaaataaa aggcaaagta tgacttaata taatcactac gtttaaccgg
    39841 taaagtagat acataataca tccatcttga gtctttttca tgtttaatat tatcagtaat
    39901 aggtgtgatt aacataaccc cagccattgc cgaactcatc aacggattaa atactgcaaa
    39961 gtatcctgca gctataatag cgactataaa ataaatatat gtttgctttc tcgttgcata
    40021 aaaactactt aggaacatac ctttcattat acttcacctc gcattatgat ttttgtagcg
    40081 tcatcaatgt gttttaaagg ctctgcattt tcaatttctg cataatcttt aactaaaatt
    40141 tgatatttcc cctttgacaa tctagaagca atgattaaat gcttaggaat ttcaacatct
    40201 ttatcttctg tcgtaacaat tccatattgt gctaaaagta tatctttctg ttcagttaaa
    40261 atcattcgtc catctttcat aaacactaat ttatcagcta aatgttctat atcttcagaa
    40321 atatgcgatg atattaagat gcctccacct tgtgcgacaa aatcttctag tatttccatt
    40381 acttcttctc ttccagaaac atccatacct gcagttgctt catctaagat taataacttc
    40441 acatcatgag aaagcgcaat cgttaaagct atcttcattc gcatccctct tgaaaaagtt
    40501 ttaattttag tttgtagtgg taactcgaaa tatttgatta aatcaaagaa tttttgacta
    40561 ttccaagtca tataaataga ttgaaatact ttatcaatat ctttaatagt caatttatta
    40621 ggcactctca aatcatcaaa cactacacct atgtgttctt tatattcgac atcatccacg
    40681 gtatgttctt ctccaaaaaa tgtgatacta ccgttatctt tatgtcgatt gcctactaga
    40741 atattaatta atgtcgattt acctgagcca tttttcccaa ttaatccaac tgtctcatta
    40801 gtagaaatcg ttaaagatgc atcttgcaat ttaaaatccg aatctttgta ttgtttgttc
    40861 acactatcta cgtttaacaa tgttgtcata tccatgctcc tctttgttta attttaataa
    40921 aaacgtcttc tcttcaataa gtaactaaag gcgttatata tgaaaatagc aatgataagt
    40981 agcaaactaa agctttgatt aattccagta gtaattgaat aaagcccaat tactacaatg
    41041 gctagtatca atattgataa gttgatcgca tatgttttaa ataatgcaat taattctata
    41101 tggcgttcac cttcatccaa tatttccaat cgcttttcag tgtaattctt atctgcaatt
    41161 tttggtattc tgtcatcaaa ccttctatta aacagtgtaa attgtgtatt gaaaatagca
    41221 ctagcaaaaa atggtattaa aaagaatagt attgcatttg ctgcagcgtg ccctaccaca
    41281 aaaataagca atactaaaaa agcaataact gtctgcagaa tacttaatat acttccattt
    41341 aaaacataac gattcgcaag caactcatat tgatctgttg catcgatatc tacctcttta
    41401 tctacaagtt gcttatactt caatgcacgt ctttgattca tcaaaacaat ggcttctacc
    41461 aatatgataa taatcgtcgc aacaatcgat attacaacga cattgttata tgtcgcaaac
    41521 gtcaagttct caataccaaa tccatcaaaa ttacctaaaa ttccacctat gataccccct
    41581 acaagtccac ccagtaaaag atatccgata tatcttagta ttttcatgcc tcatcctcct
    41641 caataataaa aacagtttcc accgtttcat tgaaaatgcg agcaattttt attgccgtta
    41701 atactgatgg cataaaattg tttcgctcaa ttagcgatat ggtttgtctt gaaacgcccg
    41761 cttgtttagc aagttgcgtt tggtttaagc catctcgtgc tcgtaattct ttcaatcgat
    41821 tacgcacatc gcatcaactc cttaattaca ctgtaaatga tatgcgtctt tttgacaact
    41881 atatttgtca aatctacacc aaaaaatatg attatccacc tatgtatgac attttgaaac
    41941 aaacacctca acgcctacaa gtcataattg tttactttcg ttacaccttc ctgcataatt
    42001 aacagcattc taattttagt atgatgcacg cattttcact aaatcaaacc attcaaagga
    42061 gactattatg gcatttacat tatctgcaat tcaacaagca catcaacaat ttactggtgt
    42121 tgactttcca aaactattca aagcttttaa agatatgggg atgacttaca atatcgtcaa
    42181 cattcaagat ggcactgcaa catacgtaca tcaatcagaa gatgatatcg ttacgtcatc
    42241 tgtaaaaagt aatcatcctg ttgctcaaaa atcaaacaaa acaatagttc aagacgtctt
    42301 aactagacat caacaagggc aaacagattt tgaaacattt tgtgatgaaa tggctgaagc
    42361 tggcatttat aaatggcata tcgatattca agcgggcact tgtacttata tcgacttgca
    42421 agaccaagct gttatttcag aattaatccc tcaataaact atatttatag caacatttta
    42481 attatttcat aaaattttat tgataatcat tatcgttcgg tataaagtaa atactatata
    42541 ctacttatga gtgaggttga ttatcatgat aactaacact tttattttag gcatcacagg
    42601 cccaacaagt cttgtcgtca ttagcattat cgctttaatt atttttggtc cgaaaaaatt
    42661 accacaattt ggccgtgcca tcagttctac tttaaaagaa tttaaatctg caacagaaga
    42721 tttagataaa gagtctcacg atacacccag taaggaatcg aaacaacagc gagagcaata
    42781 gcactgacca caccttactg gttcacttta gcgaactacg ccatcggtta gtaaaaattt
    42841 tattgtcgtt cgtcattacg gtcatcgtcg tatatgtttc atcattttgg tggatgacac
    42901 cattcataac gtatattacc cgtgcacatg tgtccttaca tgcattttca ttcacagaaa
    42961 tgatacaaat atacgtgatg attatatttt tcatcgcgtt ttgtttcatt tcaccagtca
    43021 tgttttatca attgtgggca ttcattgcac ctggattaca taataatgaa cgtcaattta
    43081 tttataaata tagttttttc agcgtgttat tattctgtgc tggcgtcgct tttgcatttt
    43141 atgttggctt tccaatgatc attcaattcg cgttaaaatt atcattaact ttaaacattt
    43201 cgcctgtcat tggctttaaa gcatatttga ttgaactcat acgttggtta ttcacatttg
    43261 gtcttctatt tcaattacca atattattta tgggtcttgc aaaattcggt cttatagata
    43321 ccacatcatt gaaacattat cgtaaatata tttatttcgc ttgctttgta ttagcaagta
    43381 tcattgcacc acccgattta acattaaata tattactcac attgccactc atattgctct
    43441 ttgagttcag tatgttcatc gttaagttca catgccgagg caaaccgcca acacattaac
    43501 cttatacgaa tcaatgctgt gattatcgtt atactgcaat accgaatatt tccaatatag
    43561 caacaaaaaa acaggcactc ggcctgtttt ttatttttta aatttaaaga ttaagatagc
    43621 aactaccata atataagcac cttgtgctat caatggttca taacttggat aaaagcccaa
    43681 ccagttaatc gttgggaatc cttcaataac atgtcttggc atcgcaccta ataattgtaa
    43741 cttttgaata cttacgccaa gcattttgaa tcccataata aaaataaaga tcgacaacac
    43801 tctaaatata tagaaaatag gtattaattt aactataaat ctaaataata atgcaaagat
    43861 gattaaaata acgatagcta aagcaatacc aataataaaa tctttggtcg ctagctcacc
    43921 tatcatcccc atatagaaaa tgataacctc gacaccttca cgcaacacag atattaaacc
    43981 aatcgtcgct aacaatacca aattaccatt actaatcgca ttagcataca tatttttaat
    44041 catgtcattc caacgttttg catttgaacg tttgtgcatc caaacaccaa cgataaacat
    44101 taatatgacc gcaacgatac ctaatcccgc ttccatactt tcacgaagaa tgccactatt
    44161 ccctaaagtt tctacaaacg taattgctaa gataatactc agtacaagtc cggcaattgc
    44221 accaccaatc acacttgcag tccctttctt atcttttaca ttacgcgtca tggtagtcaa
    44281 tgtcattaca attaacaaca cttctagccc ttcacgtaaa aagataatca tcacatcgac
    44341 gaagctataa ctatggccaa caacctcttt aatttggtta tttaaatcta ctaaaccatc
    44401 tttcacatgt gctttattat gttcgtctaa tacactttga taatatggta ttttatcttc
    44461 aattttcgta tacaaagcac cgtctttagt ttgaatttga ccttcaacat acggccaagt
    44521 ttctataaaa tgtgtaagcg cagcatcagc atccgacaat tgattgtcgt cgatagcttt
    44581 aatcgccttc tctaacgcat catttaattg tgatacatgg tattgatcat ttgcagacgt
    44641 attacttttt ttatcgacat gatcaatatt tgatttaaaa gttgtccaag catgtgacac
    44701 ttttgccgta tctaatggtg acttatgaat tgcaattcta agttgtaata atgcgacttc
    44761 aatttgtcca tattgatttg cgtcataatt gcgaatcact gtttcattac ttgtccaaat
    44821 ctgattcaaa ctattgttca aagattctaa ttccgcttta tttttatctt taatcgcttt
    44881 tgtcatcgca gcatctttag catcgacttg ctgttgcaat agtttaattt tagaacccgc
    44941 atctttacta gccaatttct cttcataagc aattaatgac ttcgttaatt gcgaaagtgt
    45001 atctttttga ttatcattcg cttttgcatc ttcaagcttt ctcacatctg atttgacagc
    45061 attactttca ctattatctt caagcgataa tttcttaact gcacttacca cttgctcaat
    45121 tgctttctgc ttattgtcat tcgatatcga attattagaa agtgcagatt tcgcatccgt
    45181 tatcacacta tatacatcac taatactttg ttgttctgct gcctgacttt tcagtaaccc
    45241 aaagctacac accatagcag cagttattag cattgctaca aatttagtca aataatgttt
    45301 caccaaggta tcctccctta ctaacacctg gtaatactaa aaatgaagca gaacctctat
    45361 gtgtaatata ttcatttaat ttatcattac tacctaaatt attttgtata tcgataaatt
    45421 gttttgtcgc tttttgaaaa gcaataaaaa gtaagcctgt ttcgaagtta cctgtgcggt
    45481 catccgtacc atccacataa ttaaaggctc tacgtaaaat tgacgtattt gcttctttcg
    45541 ctagcctcgt atgggcatct ttatcaataa tatactcgcc atgactatct ttcgctttta
    45601 agtcaatttc atcaaactct ttcccacctg ttaacggtgc accactatgt cgtttccgac
    45661 caaatgtagc ctcttgttct tccagcgcag tacgatccca cgtttcaatg tgtatttgaa
    45721 tacgtctgac aacgcaataa gttccatgtt tcgcccatcc gtcatcaata aacacataat
    45781 ctttaagttg attattcttc ctaggattaa ttgttccatc tttaaatgcc attaaatttc
    45841 taggtgtttc cttaccttta gcagagataa aaccagattg cgcccaacgt accttaacaa
    45901 tatctcgaaa tggacgaact aaattatgaa ccgcatgaaa ggatacttgc gaatcatttg
    45961 agcatgcttg aatcataata tcaccatcgc tgtaatcgtc tattaactga tcattcggaa
    46021 aatgcggtaa atctttaaag gcatcgggaa ttttactaga taacccaatt ttcttcatca
    46081 aagacttact aatcccaaag gtaatcgtta acttgcttgc acctaatcct atcgattcac
    46141 cggtatctac tggtggcatt aaaggattgt tactcggctt gccaactgta tcaccatcca
    46201 tcatacgatc agccataacc gtccactttt taaacattgc cttaattgca tctctatctt
    46261 ttgacttcaa atctaacgca acgaaattgc atgttttttg cgtgggtgtg gtaatgcctg
    46321 gttgcacttt accataaaat tcatacgcat ctttttccgg atcttctggt gtattgaaca
    46381 ttgatttgaa agaccacatg ctaccaacac cacttgcgcc aattgcaaca ccggcaccgc
    46441 caatacctaa cattttcaaa aatgtacgtc ttgaaaattg cgtactatcg ttaacttgtt
    46501 cataatttgt cattcagtaa tcacagccat tttacttagt ggctctccta atgcattaac
    46561 agcatccgca aatgctttac ggtctttctt cgatactttt tcaaatgact cataaccgcc
    46621 gttgttatct ttatatttat ccaataattg attcacttta tcgaagttca tttggatatc
    46681 atcacttaat tttttatctt ttttctctaa aataggttta aataagtcat aaattttttg
    46741 tgcgccttca acgttcgctt taaaatcata taaatctgta tgtgaataaa tttcttcttc
    46801 acctgtgatt ttagaagttg caacttcatt taataggtca acagaacctt gtaacattaa
    46861 ttttggtatg atatctaatg tatcagcttt ggcatgcaat tcttttgcat ctttcaataa
    46921 ttgttgtgca tcttttttag tcacatcatc aattttctta tcttcgtata atgctttttc
    46981 aatcttatga tatcctgacc attctttttc ctttttctct tctttcatat ctgcaagacg
    47041 tgcatcaatt ttaggatcta aatctccaaa tgcttctgca actggttcag agcgttcata
    47101 atacatacga acttttggat ataacgcttt tgcttgggcc atatcattat tttcaatcgc
    47161 tttaacaaat ttttctgtac cttttaaaaa ttcatttaac tgttcatcag tatatttatc
    47221 atatgctttt gttgcttgtt ttaattctgc tttaacacca tcatcttttt tcgatgtctt
    47281 tgaatcatcc ttcttactat cgtcgttccc acatgcagca attaataacg ttgatgctaa
    47341 taatagcgtt gttaactttt tcattttgac acctcattat agaaattcta ttatccccat
    47401 agatgtgttc ttataaatat tattttcgca ttccctacac gctaaaataa tacattacac
    47461 ttaaaacggc tgttttaaag catcctccca taaacatcat ctagttgata ataattatca
    47521 aaatcattat attgttataa aataatactg tcaacaacct tttattttta aaaattaaat
    47581 aaaagagcgc tgccatttta aatgtcatca ctcttttgta agtctaattt gtcatttttg
    47641 gtcgtattct gattttaata aactatagat gtaactcgat gaataaatac catttagtaa
    47701 ttcattgtca cgtaacatac cttctctagt aaatcccagc ctttcaggaa tagcccggct
    47761 tttttcatta ttaactgcgg cactaatctc aattcggttt aagtcaagct cttcgaaaca
    47821 atactttatc aatgcctcaa ctgcttgtgt cataatccca tgaccctcaa attctttatc
    47881 taaatagtac cctaatgaag tttttctgtg catgtgatta atttcatgta aaccgatgac
    47941 accaactagc gttccttcat accaaatgcc acactgaaat ccattaccat cagcaaattg
    48001 caaaagtcct cttttaataa atgcacgcgt atctgatggt tgctcagttg catctaccca
    48061 aggtaaccat tccctaagtg aatttcttga acgattgact aaattgaaaa gcgcttctgt
    48121 gtcatgagct tctaaaattt ttaatgttat ttgttcattc actttcattc caaacatacc
    48181 atcacatcct cattcatttt tcatataatt ctgtaaatta tcattataat aacatattaa
    48241 tgttacgcat tgtatcttaa atttgttgtc cttc
//
LOCUS       DALAAR010000010        48003 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000010 DALAAR010000000
VERSION     DALAAR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.010"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            140..2289
                     /locus_tag="O2458_000584"
                     /pseudo
     CDS             140..2289
                     /locus_tag="O2458_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2435..2794
                     /gene="sarX"
                     /locus_tag="O2458_000585"
     CDS             2435..2794
                     /gene="sarX"
                     /locus_tag="O2458_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCZ3886470.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            2984..3700
                     /locus_tag="O2458_000586"
     CDS             2984..3700
                     /locus_tag="O2458_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ3886471.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            3700..4173
                     /locus_tag="O2458_000587"
     CDS             3700..4173
                     /locus_tag="O2458_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCZ3886472.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            4265..4381
                     /locus_tag="O2458_000588"
     CDS             4265..4381
                     /locus_tag="O2458_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886473.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            4526..5170
                     /locus_tag="O2458_000589"
     CDS             4526..5170
                     /locus_tag="O2458_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ3886474.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            5286..6152
                     /locus_tag="O2458_000590"
     CDS             5286..6152
                     /locus_tag="O2458_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3886475.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            6422..7642
                     /locus_tag="O2458_000591"
     CDS             6422..7642
                     /locus_tag="O2458_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCZ3886476.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            7639..8127
                     /locus_tag="O2458_000592"
     CDS             7639..8127
                     /locus_tag="O2458_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCZ3886477.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(8226..8915)
                     /locus_tag="O2458_000593"
     CDS             complement(8226..8915)
                     /locus_tag="O2458_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCZ3886478.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            9042..9485
                     /locus_tag="O2458_000594"
     CDS             9042..9485
                     /locus_tag="O2458_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3886479.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            9552..9947
                     /locus_tag="O2458_000595"
     CDS             9552..9947
                     /locus_tag="O2458_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886480.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            10085..10384
                     /locus_tag="O2458_000596"
     CDS             10085..10384
                     /locus_tag="O2458_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886481.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            10464..11006
                     /locus_tag="O2458_000597"
     CDS             10464..11006
                     /locus_tag="O2458_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3886482.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(11102..11668)
                     /locus_tag="O2458_000598"
     CDS             complement(11102..11668)
                     /locus_tag="O2458_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCZ3886483.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(11670..12128)
                     /locus_tag="O2458_000599"
     CDS             complement(11670..12128)
                     /locus_tag="O2458_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCZ3886484.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(12131..12814)
                     /locus_tag="O2458_000600"
     CDS             complement(12131..12814)
                     /locus_tag="O2458_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886485.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(12986..13861)
                     /locus_tag="O2458_000601"
     CDS             complement(12986..13861)
                     /locus_tag="O2458_000601"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCZ3886486.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFFEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            14074..15705
                     /locus_tag="O2458_000602"
     CDS             14074..15705
                     /locus_tag="O2458_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCZ3886487.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            15702..17375
                     /locus_tag="O2458_000603"
     CDS             15702..17375
                     /locus_tag="O2458_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCZ3886488.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(17502..17945)
                     /gene="mgrA"
                     /locus_tag="O2458_000604"
     CDS             complement(17502..17945)
                     /gene="mgrA"
                     /locus_tag="O2458_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCZ3886489.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            18172..19098
                     /locus_tag="O2458_000605"
     CDS             18172..19098
                     /locus_tag="O2458_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ3886490.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            19201..20109
                     /locus_tag="O2458_000606"
     CDS             19201..20109
                     /locus_tag="O2458_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCZ3886491.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            20144..20431
                     /locus_tag="O2458_000607"
     CDS             20144..20431
                     /locus_tag="O2458_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886492.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            20714..22267
                     /locus_tag="O2458_000608"
     CDS             20714..22267
                     /locus_tag="O2458_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCZ3886493.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            22353..23726
                     /locus_tag="O2458_000609"
     CDS             22353..23726
                     /locus_tag="O2458_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCZ3886494.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(23881..24165)
                     /locus_tag="O2458_000610"
     CDS             complement(23881..24165)
                     /locus_tag="O2458_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886495.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(24162..24779)
                     /locus_tag="O2458_000611"
     CDS             complement(24162..24779)
                     /locus_tag="O2458_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCZ3886496.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            complement(24965..25387)
                     /locus_tag="O2458_000612"
     CDS             complement(24965..25387)
                     /locus_tag="O2458_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ3886497.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            25596..26762
                     /gene="norA"
                     /locus_tag="O2458_000613"
     CDS             25596..26762
                     /gene="norA"
                     /locus_tag="O2458_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCZ3886498.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            27055..27507
                     /locus_tag="O2458_000614"
     CDS             27055..27507
                     /locus_tag="O2458_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886499.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            27681..28163
                     /gene="ybaK"
                     /locus_tag="O2458_000615"
     CDS             27681..28163
                     /gene="ybaK"
                     /locus_tag="O2458_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCZ3886500.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            28413..29177
                     /locus_tag="O2458_000616"
     CDS             28413..29177
                     /locus_tag="O2458_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ3886501.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            29174..30094
                     /gene="pfkB"
                     /locus_tag="O2458_000617"
     CDS             29174..30094
                     /gene="pfkB"
                     /locus_tag="O2458_000617"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCZ3886502.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            30100..32058
                     /locus_tag="O2458_000618"
     CDS             30100..32058
                     /locus_tag="O2458_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ3886503.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            32366..33547
                     /gene="nagA"
                     /locus_tag="O2458_000619"
     CDS             32366..33547
                     /gene="nagA"
                     /locus_tag="O2458_000619"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCZ3886504.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            33768..35117
                     /locus_tag="O2458_000620"
     CDS             33768..35117
                     /locus_tag="O2458_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ3886505.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            35339..36178
                     /locus_tag="O2458_000621"
     CDS             35339..36178
                     /locus_tag="O2458_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ3886506.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            36310..37293
                     /gene="csbB"
                     /locus_tag="O2458_000622"
     CDS             36310..37293
                     /gene="csbB"
                     /locus_tag="O2458_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCZ3886507.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(37365..38420)
                     /gene="saeS"
                     /locus_tag="O2458_000623"
     CDS             complement(37365..38420)
                     /gene="saeS"
                     /locus_tag="O2458_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCZ3886508.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(38420..39106)
                     /locus_tag="O2458_000624"
     CDS             complement(38420..39106)
                     /locus_tag="O2458_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3886509.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            complement(39081..39554)
                     /locus_tag="O2458_000625"
     CDS             complement(39081..39554)
                     /locus_tag="O2458_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ3886510.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            complement(39896..40336)
                     /locus_tag="O2458_000626"
     CDS             complement(39896..40336)
                     /locus_tag="O2458_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCZ3886511.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            complement(40616..41200)
                     /locus_tag="O2458_000627"
     CDS             complement(40616..41200)
                     /locus_tag="O2458_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886512.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            complement(41299..42012)
                     /gene="queE"
                     /locus_tag="O2458_000628"
     CDS             complement(41299..42012)
                     /gene="queE"
                     /locus_tag="O2458_000628"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCZ3886513.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            complement(42016..42435)
                     /gene="queD"
                     /locus_tag="O2458_000629"
     CDS             complement(42016..42435)
                     /gene="queD"
                     /locus_tag="O2458_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCZ3886514.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            complement(42437..43105)
                     /gene="queC"
                     /locus_tag="O2458_000630"
     CDS             complement(42437..43105)
                     /gene="queC"
                     /locus_tag="O2458_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCZ3886515.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            43456..44049
                     /locus_tag="O2458_000631"
     CDS             43456..44049
                     /locus_tag="O2458_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ3886516.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            44033..45184
                     /locus_tag="O2458_000632"
     CDS             44033..45184
                     /locus_tag="O2458_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCZ3886517.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            45184..45792
                     /locus_tag="O2458_000633"
     CDS             45184..45792
                     /locus_tag="O2458_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCZ3886518.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            45858..46064
                     /locus_tag="O2458_000634"
     CDS             45858..46064
                     /locus_tag="O2458_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886519.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            46152..46862
                     /locus_tag="O2458_000635"
     CDS             46152..46862
                     /locus_tag="O2458_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCZ3886520.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            46846..47850
                     /locus_tag="O2458_000636"
     CDS             46846..47850
                     /locus_tag="O2458_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ3886521.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT        16789 a         6679 c         8469 g        16066 t
ORIGIN      
        1 gtttaaaaat taaccattat atagttttat ttatttttga tttggtattc gtattgtgtt
       61 aaatttacgt ttaattaaat aagttaaata ttatagggaa tgataaaagt ggtacttaaa
      121 tatagaaaga ggtaaagtta tggcaaaatc atgcttgcat atacttacta ataatgaata
      181 tgcgacaacg cgttgccaag atggcatagt cttattttgg ccaattgacg gggaaatcga
      241 actacaaaaa tttcgtaaaa gtaaaataat tgaagatgat atatatatta ttaatcatct
      301 ggatgtattt agtattaaga ataataaaaa aacgatcatg ttgtatttga gtagcgattg
      361 gtttgcggaa ttaggcttta ctttctttaa ttaccactat acagcaaagt tgattaaatc
      421 atcctataat ttgaaatgtc tactattaaa attgacatat cgataccttg ataatcagcc
      481 tcttaatgac gctgatatta gaaaattaca ggatattatt aaaatcattg caaaagaagc
      541 aagtatggat aaaaagattg cacaaaatca atatcgatat gcgtattatg gtgatttgcg
      601 tgatgagctc gaatatattt atcaaaatgt aaatcaacga ttgacattaa aaagtgtcgc
      661 tgataaatta tttgtctcaa agtcaaattt gtcatcacaa ttccacttac ttatgggcat
      721 gggttttaaa aaatatattg atactttgaa aattggtaaa tcgattgaaa ttctacttac
      781 tactgatagt actattagca acataagtga acatttaggt tttagtagta gctccactta
      841 ctctaaaatg tttaaaagtt atatggatat aacaccgaat gaatatcgta atttatcaaa
      901 atataataaa tgtttaatgc taaagccaga accactagta ggcaaaatgg tgcaagaagt
      961 aaaagaaatc atattgaatt atattgaaca ttataaaaac cacctaactg atgttataca
     1021 tattgatgaa gacaaatttg aaacacctaa attgtttcaa acggttattc aaataaatac
     1081 ttatacagaa atgaaattag ttttcttaga aggaatcttt aaaaccttat tgaataagaa
     1141 cagtcaagtt gtctttttca tcatgccatc gattctaaaa agtaaaaata ccatgtccga
     1201 agaagaaaaa ttcacaatca ttaaaacaat aattgaaagt gatctaaaga tagcatttaa
     1261 tataaataat attgaaacaa cttattttgt tgaagaagct tttatgagtg ttttcagaca
     1321 aatatctcca aacgaattaa gtaatcataa taattacgaa gtgcattttg tttttgattt
     1381 atcattgatg gaaattagaa caatttatcg aatgatatta aaattacata acatcatgtt
     1441 gaatgtgaaa ttaggattga acattacctg tttatttgaa aaaccttcag tttttaaatc
     1501 actagtatca caaataaagc gacttaaatt cgattcgtta ataatagata atgcaaattt
     1561 aagtagccct tatttgatgg gggaaagtga tgagttacta ttgaaaaata ttttgcattt
     1621 taaaaattta aaacaagtaa ttaatgaatt ggatattgaa caagaaaagc tgatttttct
     1681 aaatgttgaa aatcataaac tgcttaataa taaagaacga gatctaagta atagtgctcc
     1741 attaatttat aagacattaa gtgcgctgta tcacaacttt gatggctttg gattaaacat
     1801 ttttgacaat catcaagcat ttaatgcgat gcatctatat gacaaaaatg gatttaaaac
     1861 aacactaggt cttatattag aaaagtttat cgaatatgtc tcgaaaccaa aatacgaaaa
     1921 cagttattat tctatttttg atatagagaa ttattattgt cttgttattt atgattggcg
     1981 agtgatagag agcgagacaa ttatgagtaa ttttgaggat agtcaagttt atataaattt
     2041 taaaaacaat gttttaaacg ataaatatct aattgtaata gaaacattgg acaaaatagt
     2101 ggcaacatta atcatttgat ttctaaagaa ttaagagata aatatgaatg gaaccctagt
     2161 ttactatcta aaattgacaa ctaccttaaa ccagcaatag agattaaaga gcataatttt
     2221 agtgataatt ccttgaatat taacgttact tttaatgcgt tatacataat taaaatagga
     2281 aaaaaataac accttgatat gtattgcaaa atttaatttg catttgttgg taattttgac
     2341 aacgtattaa aaacaaatga gaaagacaat ggctgaaaaa tttaagataa taatgacaga
     2401 agcattgtct ttatatattt gggggtgcaa cattttgaat actgagaaat tagaaacatt
     2461 gcttggcttc tataaacaat ataaagcatt atctgaatat attgataaaa aatataagtt
     2521 gtcgctaaat gatttagcag tcttagattt aacgatgaag cattgcaaag atgaaaaagt
     2581 acttatgcaa tcatttttaa aaactgcaat ggatgagcta gatttaagta ggacaaaatt
     2641 attagtttct ataagaagac taattgaaaa agaaagactt agtaaagtta gatcatctaa
     2701 agatgagcgt aaaatttata tttatttaaa taatgatgat atatctaaat ttaatgcttt
     2761 atttgaagat gtagaacaat ttttaaatat ttaattgaaa ttgagtgtcg aaagcataga
     2821 atttgcttat cggcacattt ttaatttata catattttaa aactaagtaa cagtttgaag
     2881 aaatcgtagt tcaataatgt taattgtgaa aatgtatata aacataaaaa aatcatgtat
     2941 aatatatgtt gttaattaaa cagttcgaaa gcgagatgac attatgggac gtaaatggaa
     3001 taacattaaa gaaaaaaagg cccaaaaaga taaaaacaca agtagaatat atgcgaaatt
     3061 tggtaaggag atttatgttg cagcaaaatc tggtgaaccc aatccagaat ctaaccaagc
     3121 tttaaggttg gtgcttgaac gcgctaagac atattcagtg ccgaatcata ttattgaaaa
     3181 agcaatagat aaagctaagg gtgctggaga cgaaaacttt gatcacctaa gatatgaagg
     3241 atttggccca agcggatcaa tgctaattgt tgatgcgtta acaaataatg taaatcgtac
     3301 tgcctctgat gtgcgagctg cttttggtaa aaacggcggt aatatgggtg tatctggatc
     3361 agttgcttat atgtttgatc atgtggcaac atttggtatt gaaggaaagt ctgttgacga
     3421 aatacttgaa acattaatgg aacaagatgt agatgtaaat gatgtgattg acgataatgg
     3481 attgacaata gtctatgctg aaccagatca atttgcagtc gttcaagatg cgcttcgtgc
     3541 agcaggtgtt gaagaattta aagttgctga atttgaaatg ttacctcaaa cagatattga
     3601 actttctgaa gcggaccaag taacatttga aaaattaatc gatgcattag aagatttaga
     3661 agatgtacaa aacgtattcc ataatgtgga tttgaaataa tgaaatcagc agaacaatgg
     3721 attgatgaat tgcaacttga atcacatcct gaaggtggtt tctatagaga gacaattcga
     3781 gaagtattga aagatggacg cagagcgccg tttagtagta tttatttttt acttacagat
     3841 gacaatattt cgcattttca tcgaattgat gctgatgaag tatggtacta tcatgctggc
     3901 gattctctaa caattcatat gataaatccg gatggggaat atacgactgc aacattgggt
     3961 actgatatcc aaaatggaga tgtattgcaa tatgtagtgc ctaaaggaac aatttttgct
     4021 tcttcaatcg aaatatcaaa tacttttagt ttagtaggtt gtatgtgtca accggcattt
     4081 gagtttaagc agtttgaatt gtttaagcaa tctgaattaa ttacgcaata tccgcatctt
     4141 aaatcagtaa tcgaaaaata tgctttaaaa taaaagtgat caatgaagtg gtttgaaggt
     4201 tgttaataaa cctttgagtc acttcatttt tatatgtatt cttgattgaa tcagaataga
     4261 tttgatgctt cagctgtttt taatgaaaat agcattaaat gattttgaaa acgataagag
     4321 tgtgttattt atatttttga aaaatcactt ttatgaagaa atgtgtgtac tatctaatta
     4381 atattgtcta ttttaagtaa attgaagcga gttgtagtaa aataataata aatattttca
     4441 tatgattaac aaaaactata aaactgtatc atgacatgac atcatttatg tagttttata
     4501 taataatgac aaggagttgg tgactgtgaa agtaaagtat atagataaac gtcactggcg
     4561 tcgcctaatt gatagggaat acacagaggt aaaagttaat aataataggt ttaagggtat
     4621 tataggctta gtcacgatga aaaaggttcg tgatccttta gaggtgacgg tagttggaca
     4681 aaatatcatt gtcgcagatg acaattataa atggttgcaa atactacctg aaaagaaacg
     4741 ttatagtata actgtaatgt ttgataataa aggcaatcca ttagaatatt attttgatat
     4801 aaatatcaaa aatataacgc aaaaaggtaa tgcgcgtaca gtagatttat gtttagatgt
     4861 tttagcatta ccaagtggtg aatatgagtt ggtagatgaa gatgatttaa tgtttgcatt
     4921 agaaagtgag caaattacaa aaaagcaatt tcatgaagca tatatgattg cacatcaaat
     4981 tatggcagag ttagaaaatg attttaaagg attccaaaag aaaatcatgt actgctttaa
     5041 taaaattaat gcaaaggctc aaaaaaatca tcaaaagcca caaaataaaa ctaatattga
     5101 aaaaagcaaa caaataaagc ctaagcaata taatcaaact aaaaatcacc aacaacaaaa
     5161 gaaaaactaa gtaattcaag ctgcagccat accaataaaa ttggtgaaat tattcgttag
     5221 taatgaactc atgaatgctt gtagccatga aagttcaata attgaataat ttatgggggg
     5281 aaattatgaa gattgaagac tatcgtttac taataacatt agacgaaacg aaaacgttac
     5341 gtaaagcggc tgaaatttta tatatatctc aacctgctgt tacacaaaga ctaaaagcta
     5401 ttgaaaatgc ttttggagta gatattttta tcagaacaaa aaaacaattg attacaacaa
     5461 ctgaaggaac aatgattatt gagcatgctc gtgacatgtt gaaaagagag cgattatttt
     5521 ttgacaaaat gcaggcacat attggtgaag tgaatggaac aatatcaatc gggtgttctt
     5581 ctttgattgg acaaacctta cttcctgaag ttttgagcct atataatgcc caatttccta
     5641 atgttgaaat acaagtgcaa gttggttcaa ctgaacaaat taaagcaaat catagagatt
     5701 atcatgttat gataactcgt ggaaataagg taatgaattt agctaacaca catttattta
     5761 atgatgatca ttattttatt tttccaaaaa atagacgaga tgatgttaca aagttaccat
     5821 ttatagagtt tcaagctgat ccgatttata taaatcaaat aaaagaatgg tataacgata
     5881 atttagaaca agattaccat gcaactatta cagtggatca agtagcaact tgcaaagaaa
     5941 tgttgattag tggtgtaggt gttacaatct tgccagaaat tatgatgaaa aatatcagca
     6001 aagaacaatt tgagtttgaa aaagtagaaa ttgataatga accgctgatt cgttcgacat
     6061 ttatgagtta tgatccgagc atgttgcaat tgccacaagt tgattctttt gtaaatctca
     6121 tggcgagctt tgttgaacaa ccaaaggcgt agttttagac taatttaagg tttgtattta
     6181 attttaaact attcggttaa attgaacgta gttggttgct aatgcaccaa cagcaaaaga
     6241 gcccctaatt aataaattaa aaggggacaa aggaatacag ttggttgcta atgcaccaac
     6301 tgcataagag cccctaatta ataaattaaa aggggacaaa ggaatacagt tggatgctaa
     6361 cgcaccaact gcataagagc ccctaattaa taaattaaaa ggggctctaa gaagcggggt
     6421 tatggatatg tttgcagcgt tattacaaat aaagaattat aaactctttg ttgctaatat
     6481 gtttctacta ggtatgggta ttgcggttac ggtcccatat cttgttcttt ttgcaactaa
     6541 agatttaggt atgacaacaa atcagtatgg attacttcta gcatctgcag cgattagcca
     6601 gtttacagta aattcaatta ttgctagatt ttcggatacg catcacttta atagaaaaat
     6661 tattattatt ctcgcattat taatgggtgc gcttggtttt tcaatatact tttttgtaga
     6721 tacaatctgg ttattcatat tactatatgc gattttccaa ggattatttg caccagcaat
     6781 gccccaactt tacgcatctg ctagagaatc tatcaatgtt tcaagctcta aagatagagc
     6841 tcaatttgcc aacacagtat tacgttcaat gttctcattg ggctttttat ttggtccatt
     6901 tattggtgcc caattaatcg gattaaaagg ctatgctgga ttgtttggtg gaacaataag
     6961 tatcatttta tttactttag tacttcaagt gtttttctat aaggatttaa acattaaaca
     7021 ccctattagt acgcaacaac atgttgaaaa aattgctcct aatatgttta aagacaaaac
     7081 gcttttatta ccatttattg catttatttt attacacatt ggacaatgga tgtatacgat
     7141 gaatatgcct ttatttgtta ctgattattt aaaagaaaat gaacaacatg tcggttattt
     7201 agctagttta tgtgctggtt tagaagtgcc atttatgatc attcttggcg ttttatcatc
     7261 tagattacag actcgaacat tgttgattta tggagcgatt tttggtggtt tattctactt
     7321 cagcattggg gtatttaaaa acttctatat gatgttagca ggacaggtgt ttttagctat
     7381 tttcttagcg gttcttttag gaattggtat tagttatttc caagatatct taccagattt
     7441 tccaggatac gcttcaacac tattttctaa tgcaatggtt attggacagt taggcggtaa
     7501 cctattaggt ggtgctatga gtcactgggt aggtttggaa aatgtatttt ttgtatcagc
     7561 agcatcaatc atgttaggta tgatacttat attctttact aaaaatcaaa aaattacaaa
     7621 agaggatgtg atatcaacat gacaattatt ttatggctac ttatcatcgc tgccttcatg
     7681 ttagcatttg ttgggttgat taagccgatt attccttctg ttttagtatt atgggttggc
     7741 tttttaatct atcaatttgg ctttcataat cagcatttat catgggtgtt ttatgtatct
     7801 atggcattgc taacaatatt aattttatgt gccgactttt tagctaataa atattttgtg
     7861 aatcgcttcg gtggttctaa gtttggagag tatgcagctt taattggtgt ggttattgga
     7921 tgttttgttt taccgccatt tggaattatt attatacctt ttattttggt attcatagtt
     7981 gaattaatac aaggctattc atttgaaaga gcagttaaag taagtatagg ttcaatcgta
     8041 gcatttttaa caagtagtat agctcaagca atcattatgt ttataatgat tgtatggttc
     8101 tttatagatg ctttattgat taattaataa aaagcttatt gcaaaatatg tttttcggta
     8161 actgtaattt agtgatttta tcattaacag taccaaattc gcatatgatg taataagctt
     8221 tttgtttata aaaatgtatg agaatgtttt ttcgaaatat ttctttcaat gcgtaatcca
     8281 ataggcataa ctataaatga aaatataata aatacccaat tgcttacctg aatatatggt
     8341 ccaaaggcaa gtaatgattg tggaataaag aatacataga aaaatcctac aattagtaga
     8401 atgattgcta aataggtgaa tatccatatc caatttgaat tgttaaaaca ctgcagttgt
     8461 attgttttaa aactaaacca tataaataaa aagacaataa agaatccaac gactactgaa
     8521 aacgggaatg aaacaaaata taaattactt ccattttttt ccatgaaaaa tcctaaaaat
     8581 cctttgagaa aactaacaat cccaattaat agaacgatgt gttgatagat atatttaaaa
     8641 atatttttaa atgtttcatt aggcatcgct tttagttctt ttattgcatg tgcttttggg
     8701 tcgtgattga aaaaatctaa ggctaataaa ccatgttgtt ctgcgcttaa taattgtttg
     8761 agtatacggt taataattaa ctctgtatca tgagggttga cgcgaaagtc agagcgcata
     8821 taagtcatat aattctcgaa gatttctcta tcagtattgc ttaatcttaa tgatttaaca
     8881 ttattttctt ttgttaattg cgcagtactt ttcattgtta cttaagcgct cctttaaaaa
     8941 tgtttaattc caaattaaaa tggaaatgat tttatagtat taataaggtc aatcatatca
     9001 tattaaacgc ataaatataa cgattaatat tggagaggaa aatgaggaca cttaataaag
     9061 atgaacataa ttatatcaag caaatagcta atatacatga gacattattg tcgcaagtag
     9121 aatccaacta taaatgtact aaactgagta ttgctcttag gtacgagatg atatgttcaa
     9181 gattagaaca tacaaatgat aaaatttata tatatgaaaa tgaaggtcaa ttaatagcgt
     9241 ttatttgggg acattttagt aatgaaaaaa gtatggttaa cattgaactg ctatatgttg
     9301 aaccacaatt tcgcaaactg ggaatagcta cgcaactgaa gattgcgctt gaaaaatggg
     9361 caaaaactat gaatgcaaag cgaataagca atacaattca taaaaataat ttgccaatga
     9421 tatctttgaa taaagattta ggttatcaag tgagtcatgt gaaaatgtat aaagatattg
     9481 attagaatta ggattatgtt gctaattcat gttaaaatta aaaaagattt aatgacgtta
     9541 aggagtttta tatgaagaaa ttaatcatca gtattatggc ggtcatgcta tttttaacag
     9601 gttgtggtaa aagtcaagag aaagccactc tggaaaagga tatcgataat ttacaaaaag
     9661 aaaataaaga attaaaagac aaaaaagaaa agcttcaaca agaaaaagaa aaattagcag
     9721 ataagcaaaa agaccttgaa aaagaagtga aagatttaaa accttcaaaa gaagataaca
     9781 aggatgataa aaaagacgaa gacaaaaata aagacaaaga taaagataaa gaggcatcac
     9841 aagataagca atcaaaagat caaactaagt catcggataa agataatcac aaaaagccta
     9901 catcagcaga taaagatcaa aaagctaatg acaaacacca atcataatcg aattgcttac
     9961 ttgttataga tgaaaggtac agcgttttaa accttatttt aagggtatgt attaattaaa
    10021 atgtggtcat gattgaaaca gaacgtaaaa atagacaacg taattaataa aggagagaaa
    10081 cggcatgcat gaacaagatt ttagaatttt agagggtcaa gatattactt tgccagaatt
    10141 aggtagagaa ttagaaaata ttacaggaca tacgattgct gattctactg gcgaaattaa
    10201 gcgtgtaatt gcacatttac caaactttga gtccgataca gatacttttg ttgctacata
    10261 tcgtttaaac catcaacaag attttataga tgcaactttt actgcgctga aatcagatag
    10321 agcacgttta aaagaagtgc cagttcatgt tgaacttata agttatattt ctaaatcaaa
    10381 ataaactgct atctaaaacg caaagttgat caaaatatcg attttgtgtt ttttattgag
    10441 aaattatata ggagtgtcaa tcgatgattt attgtgaaac agagcgttta atattaagag
    10501 actggcatga agatgatctg ttaccttttc aaaaaatgaa tgcgaattat gacgtacgta
    10561 aatattttcc aagtttattg agttatcgtc gttcagaatt agatatgaga actatggatg
    10621 cggttattaa agattatggc attggattat ttgctgtaga agataaagag tcccatcaat
    10681 ggataggctt tataggtttg aattatattc cagaaacaag cgattatcca tttaaagaat
    10741 taccgcttta tgaaataggt tggcgcttgt tgccagaatt ttggggaaaa ggattagcaa
    10801 ctgaaggcgc aaaggcaaca ttgaagttag cagaagaaca tcaaatatac gatgtctata
    10861 gttttacagc agaagcaaat aaagcttcac aacgtgtaat ggaaaaaatt ggcatgacag
    10921 tgtatgatca tttcgaatta cccaatctaa gtaagtatca tttattaaaa aggcaagtgc
    10981 gctattacat taatcttcga aagtgaaaaa tttatacata agcgtaacaa acacccctaa
    11041 cattgtttag ctgatgataa caatattagg ggtgtttgtt tattttttaa ccttagaatg
    11101 attaatcgta tgaacgagta cccagaggtt tgaaatttaa tattgattca attaatgatt
    11161 ccttagtgtc gcataacggt gcaagagcac gatacttagg atcaataaaa ccttcctcaa
    11221 tcatatggtc aatcattgtt tgtagtggat tgaaaaagcc attaatatta taaatggcaa
    11281 taggcttttc atggatacct atttgagccc aactatacat ttcgaaaaat tcttctagtg
    11341 aacctgcgcc accaggagcc atgacaaatg catctgcaag ttctgccatt ttatttttac
    11401 gttcatgcat agaatcaact aaaattaatt cagttaaacg ttggcttgtg atttcatgtt
    11461 catctaacat tttaggcatg acgccaatag ctttgccgcc atgatctaat acaccatctt
    11521 gaatggcacc cataatgcca attgaccctg caccaaatac taattcataa ccttgttcag
    11581 caaaatattt acctaaatcg tatgcttttt gtacatatga agggtcatga cctttgcttg
    11641 caccacaata aactgcgatt cgtttcatgt taatccagct ccttaattcg atgaatgact
    11701 tttaatagtg attgttcaaa cactttttga tcttgctttg taaaaggtgg gggacctttg
    11761 tggcgaccac cttgttttct aatttgtgca ttcatatatc gtttatctaa tagttgttga
    11821 atatttttgg aattgtatat cttcccatta tgatgcatga caattaagac tttgtcgact
    11881 aataaacttg cgagtccata atcttgagtg actacgatat catccttcgt tgataattga
    11941 acaattttgt aatcaactgc atctggtcca tcatcaacat ataatgttga tacatgtgga
    12001 ggatataatt ggttcgaaaa atggctgaag ctccgaataa ttgtcacaaa aatgcctgtc
    12061 tcagttgtta aatctataat agaatcaaca acaggacaag catctccatc aataataata
    12121 tgtgtcacaa ttatgcctct gtattgtttt ctttattttg ttgagaggcg cttttggcaa
    12181 cataatcttt atatttttta aatgacttga tgcgtgcttt atcagcttct tgttggcgtt
    12241 tttgttcttc tttgtgtcgt ttttcaatat ttttttgtaa ctttttattc attttagcga
    12301 tttctttgcg atttttttca gctagtttat cgcctttttt ctcagttttc tcatctaatt
    12361 tattaggtgt taagcctgct ttttcttcgt atttttgtga ttttttcata tctttaatac
    12421 gttgtatttc attcttttcg cgggcttttt gctcttcttt atgacgcttt tcgatatttt
    12481 tttgaagtat tttattcatt ttatcagcgt ctttacgatt ttgtttagct aatttttcgc
    12541 cttttttctc aatataggca ggatcatgtt ctctagcaaa ctttttaagt tcacgtttat
    12601 tttcaaaatc ttgtttttta tcgccgacat attctttaac atcactcgct gtgttactga
    12661 ttgctgcaga tgtttttgaa gcaactttac ttgtagcatc tgtaactttt tgtacgtccg
    12721 gatgttgttt gatacgttta cgttcaacaa ttaacggtac caatacaatt ggtaatacat
    12781 taatcataaa tttgatgact tttttcttat ccatagatct tgcctccata attactttat
    12841 taattttaca taccctatga tacatcaata taaacgatga tagtagtgaa tcactattaa
    12901 gtatttcaga tgttttttaa aagaagacaa taaaaactgc caatcaagtg attccttaat
    12961 tgacagtcta tattttaaaa ggaaattaaa tacctttacc aatgccaaat ccgaagtaaa
    13021 gtatagcaat aaagattact aatacaattc tgtaaatggc aaatggaatt agtttgattt
    13081 tgttaattag atgcaagaat gttttgattg caattagtcc aacagtaaat gcagctaaaa
    13141 agcctaaaat ataaaaaggt atatcagcaa tctgaatatc ttgataatgt tttaataaag
    13201 ataaaccact agctgctaac ataattggaa cagccataat aaatgtaaag tccgatgctg
    13261 ctttatgatt taatttcatt aataccccag ttgaaattgt tgagcctgaa cggctgaaac
    13321 caggccacat agctactgct tgagaaatac caattacaaa tgcttggaaa taactgattt
    13381 gatctactgt ttgtgggttt ttaactttag ctgagtattt atcagcaata atcatataga
    13441 tagcacctac gaataagcca atcataacag ttggcacact aaataaatgt tcttcgaaga
    13501 aatcatcaaa tagtaagcct aaaatacctg ctggcaccat acccactaat acatgtaata
    13561 aatttaaacg tcttggcttt gaacgtcttt gttgatcgtt atctccttca acatgtttgt
    13621 gtttaccaat atgtaaaatc tctaagaagc gttcgcggaa cacccatgct gctgcaaaga
    13681 cggatcctaa ttggatgacg attttaaatg taaatgctga ctgagaacct aaaaattcag
    13741 atgattttaa ccacatatca tcaactagga tcatatgtcc agtagaggaa acaggtgcaa
    13801 attctgttaa tccttcgacg acccctaaga taataccttt tattaattca atgataaaca
    13861 taatgtaccc actttcatta ctcaatttaa tttatttaaa tatcaaaatt accatatcat
    13921 gatagcatat tcatttaaag acatgctagt tataatacta gactaaagat gtatatattc
    13981 attttctttt acatgtaaaa ctacaatatt ttattgagct atttaatttg attttaagga
    14041 aaacctttta taataggttt aggtgatata attgtgaaaa aattaacaac aatactgttt
    14101 caatataaaa tttttccggt actcatgttc ttggtcagta ctggtctcgg cataatcgtt
    14161 ataacgcaaa atattttaat agcagatttt ttagctaaaa ttataagaca tcaatttcaa
    14221 ggtttatgga ttgtattatt tattttatta ggtgttttac ttttaagagc aactgtgcaa
    14281 tttctaaatc aatggttagg tgatacatta gcatttaaag ttaagcatat gcttagacag
    14341 cgggttattt ataaaaataa tggtcatcca atcggtgaac aaatgactat actcacagaa
    14401 aacattgatg gtctagcacc tttttataag agttatttgc ctcaagtgtt caaatcaatg
    14461 atggttccgc tcatcataat cattgcaatg tttttcatcc atttcaatac cgcattaatt
    14521 atgttaataa ctgcaccatt tattcctttg ttttatatta ttttcggttt gaaaacgcga
    14581 gatgagtcaa aagatcaaat gacttatttg aatcaattta gtcaacggtt tttaaatatt
    14641 gctaaaggtt tagtgacgtt aaagctattt aatcgtacag agcaaacaga gaagcatatt
    14701 tacgacgata gtactcagtt tagaacttta acaatgcgca ttttacgcag tgctttttta
    14761 tcgggattaa tgctcgaatt tataagtatg ttaggtattg gattggttgc attggaagca
    14821 acgctaagct tagtagtatt tcataatatt gattttaaaa ctgcggcaat tgcgattatt
    14881 ttagcgcctg aattttataa tgcaattaag gacttagggc aagcgttcca tactggaaaa
    14941 caaagtgaag gtgccagtga cgttgtgttt gagtttttag aacaaccgaa ctataataat
    15001 gaatttctat taaagtatga ggaaaaccaa aagccattta ttcagttaac agacatatca
    15061 tttcgatatg atgattctga tagattggta ttaaatgatt taaatttgga aatatttaaa
    15121 ggtgatcaaa ttgcacttgt aggtccaagc ggggcaggta aatccacttt gacacatctt
    15181 attgcaggtg tttatcagcc aacaataggt actataagta caaaccagcg tgatttaaat
    15241 ataggaatac ttagtcaaca gccatatatt ttcagtgctt ctataaaaga gaatattacg
    15301 atgtttaaag atatagaaaa taatactatt gaagaagtgc tagacgaagt aggtttatta
    15361 gacaaagtgc aatctttcac aaaaggcatt aacacaataa taggtgaagg aggcgaaatg
    15421 ttatctggtg gacagatgag acgcatagaa ctttgccgtc ttttagttat gaagccagat
    15481 ctcgttatat ttgatgagcc tgcaactggt ttagatattc aaacagaaca catgattcag
    15541 aacgttctgt ttcaacattt taaagataca acgatgattg tcattgcaca tagagataat
    15601 acaattcgcc atttacaacg acgcttgtat atagaaaatg gaagactgat tgctgatgat
    15661 cgcaatattt cagtaaatat aacagaaaat ggtgatgact tatgaaaaca cgactaaaat
    15721 ttcaagtaga taaggattta ttgttagcta tagttgttgg tgtttgtgga agtttagttg
    15781 cgctcgccat gtttttctta agtggttata tggtgacaca aagtgcactt ggtgcgccac
    15841 tatacgctct gatgatttta gtcgttacag taaaattgtt tgggttttta agagctatta
    15901 ctcgatacgt agagcgcctt atttctcata aagctacatt tacaatgcta cgtgatattc
    15961 gggtacagtt tttcggtaaa ttagtaaatg tcattcctaa tgtttaccgt aaactgagtt
    16021 ctagtgattt aatttcacgt atgattagtc gtgttgaggc attacaaaat atatatttac
    16081 gtgtttatta tccaccagtc gtcatcggtt tgacagcgct agttacagtc atagttttgg
    16141 cgttcatttc aatcggccat gcgctattga ttatggttag catgttgttc actttactca
    16201 ttgttccttg gttaagctca aaaaaagcac gtactttaaa gaaacatgca gctaatgaac
    16261 aggcccgatt tttaaatcat ttttatgatt ataaagctgg tatggatgaa ctacgtcgat
    16321 ttaatcaaat taatcattat cgagataatt tgatggctaa attaaatcat tttgataaat
    16381 tacaacttaa agagcaacgc tttttaacga tttatgattt tatattaaat attattgcta
    16441 tgctttcgat ttttggtagt ttagttctag gattaattca aattaatgca ggccaactaa
    16501 atattattta tatgacgagt atagttttaa tggtcttaac tttatttgaa caagctgtac
    16561 caatgacaaa tgtcgcgtat tataaagcgg atactgacca agcattgcac gatattaatg
    16621 aagtgatatc tgtaccttct actaatggaa aaaaacgtct taatgataag tatgatgcaa
    16681 cgaacattta tgaagttaag gatgctagtt ttaagtattg gaatcagcaa acgtatgtgt
    16741 tgtcggatat taattttaat gttaatagag gcgaaaagat tgcgattgtg ggtccttctg
    16801 gttcaggaaa aagtacatta ctacaaatta tggcagggtt atatcaatta gatagtggct
    16861 ctgttcgttt cgaaaatatg gatatgtttg aaatagatga caaagataag tttgaatcgt
    16921 taaatgtctt gctacaatct caacaattat ttgatggtac aatacgtcaa aatttattta
    16981 ccgatgaaaa agatgaagcg gtgcaagcaa tatttaagca attagattta gaacatttgg
    17041 cactagaacg tcaaattgac ttagatggtc atacattatc tggcggagaa attcagcgtt
    17101 tagcgattac gaggatgtta ttaaaagata ctgcatcaac atggatttta gatgaaccaa
    17161 caactgcatt agataaacaa aatagtttaa aagttatgga tttaattgaa gcacatgcag
    17221 aaacattaat tgttgctaca cacgatttaa ctttattgtc acgttttgag accatcattg
    17281 tgatgataaa tggtaaaata gttgaaaagg gaaactatca acaattacta gctaatcaag
    17341 gtgctttatg gaatatgatt caatataatg cataaaaaaa ctgcttgata ggctaagaaa
    17401 cctttcaagc agttttttaa ctaacaagct tacgcgtgct aattgttttt ctattcttaa
    17461 taaattctaa acattacttt aattgtcatg acaaaagtta attatttttc ctttgtttca
    17521 tcaaatgcat gaatgacttt acctaataag cgattaagtt ctttaacttc atcttgcgat
    17581 aaagaagaag ctgaagcgac tttgtcagat gcattactta attctggtct aatagtttca
    17641 cttttgtcag tcaagtgaat aaatacttca cgttgatcga cttcggaacg ttcacgctta
    17701 attaagtcta cttgttccat tcgttttaat aatggtgata ctgtaccagt atcgagtgct
    17761 aattcagtta cgactttctt gacgtttaca ggagattcat cccataaaat tgttaagaca
    17821 agaaattgtg ggtatgttag attgtacttc ttaaaaactt tgttagagta gtagcgatta
    17881 acttgtcttt gagcattgta caaactaaag catagctgtt cttttaaatt atgttgatca
    17941 gacattaaag ttctcctcca gacatactat ccgttttttt ctcttttcgg attggtaatc
    18001 attaaaaagt tgattgttta ttaattcaca actttctttg attcaatgcc atgctaaaat
    18061 taaagtatgt ttaaagttta gaagatattt ttgattaaat caagcaaaaa gataatttaa
    18121 tatatatgtt atcattttta aaaataactg taatagaaaa gagaatataa aatgaaaaat
    18181 aataaagatg aaaaaataag aatatccata attaacggat ttttgggtag tggtaaaacc
    18241 acgttactga cacattatat tagtgaatta ttaaaaaatg atgagaaaat taaaatcatc
    18301 atgaatgaat tcggtacttt tgatattgat agcaatagta tttcaaatga aattgaagtc
    18361 cattcattga ttaatggttg tgtttgttgc gatcttaaac aagaacttgt ctatgaacta
    18421 aaagccattg ctttaaaagg ggacgttaat catgtcatca tagaagcgac aggcattgcg
    18481 catcctttgg aattactagt tgcatgtcaa gatccgcaaa tcgttaattt ctttgaaaag
    18541 ccgattattt atggtgtatt agatgcgact cgatttttag aacgtcatca atataccgaa
    18601 aatacagttt cgctgatgga agatcagttg aaactaagtg acatgattat tattaataaa
    18661 attgatctta taactgatga cagtcttgag aaaattgata agcaattagg tatgatttgt
    18721 gcaagtattc caacttataa aacaacctat ggaaaagttt cgttggaaga attggactta
    18781 actgttaaag acagagagat atcgtctcat catcaccatc atcatgggat taaaagtatg
    18841 acttacacgt ttacaggtcc gattgatcgt catttgtttt atcaatttat aatgaaatta
    18901 ccggaatctg ttctacgttt gaaaggttat gtgtcattta gagatcaacc aaatgcaatt
    18961 tatgaatttc aatatgcata tggtttacca gactatggaa taattggcat gcaattacca
    19021 ttaacgattg ttattattgg tgaaacttta gatacaaatc acatacgtaa tcaattggat
    19081 atgctacaat ttacgtaaat cagaaaatgt gttgtttcaa tttaacggaa atagcacatt
    19141 aatttcttaa tacttttatt gtgaatattt tgtgacattg agggatggag tggatgagat
    19201 atggaacaaa taatgattaa tcactatgtt catttttcta ggcttgtaca aggtttttgg
    19261 cgtgcaaatg aatggaagat gactgcgaaa gagttaaatt attttataaa tgaattagtt
    19321 gaacgtggaa ttacaacgat ggatcatgct gatatttatg gagattatca atgtgaatca
    19381 ctgtttggta atgctttgga tttatcaccc gaattaagaa ataaaattca aattgttacg
    19441 aaatgtggta tcattttgcc ttctaagcaa tttgatttta caaatggaca tcgttatgat
    19501 ttgagtagta agcacatcgt gaaatctgtt gaacagtcat taatcaattt gaatgtagat
    19561 tatttagata gtctactcat tcatcgtcct tcaccattga tggatccaga acaagttgct
    19621 gatgcattaa ctaaacttgt taaacaaggt aagttgaagt cattcggggt gtcgaatttt
    19681 aatcattcac aataccaatt gttaaatcaa tatattatga aagaaagact acatattagc
    19741 atcaatcaat tagaattatc gccatatcac gttgatagtt tacaagatgg aacaatggat
    19801 tcaatgtatc aaaaccatgt tcaaatcatg gcttggagtc cttttgcagg cggtaaaatt
    19861 ttcgacaagg aagatattaa agcgcaacgt attatgaaag ttgttcaatc aatagctgac
    19921 aaatatggtg tgagtgacac agctgtgatg atagcgtggt tagtaaaaat accgcatcgt
    19981 atcatgccga tacttggaac aagtcagtta aagcgtattg atcaagcaat cgaagggcta
    20041 caacttaatt tagatgatca gtcgtggttt gacatttaca ccgctattat cgtacaagat
    20101 attccgtaaa cttatttact tttaaatcat aaaggagcat atcatgacaa acgaagataa
    20161 acgtttcgaa caattaagat ttgaacgcaa atttatagtt attccgtatt taatttatgc
    20221 agtcattgta ttactattaa atattttcta ttctgatttg aaaataacaa tgacattatt
    20281 cggacttttc tttgcgtata atgtagtcat tttgttcata gcatttgtta aacattataa
    20341 acgcacattg ttactaagtc ttatattaac agtgcttagt ggcgcggcat tctttggaat
    20401 tatttatgtt tatggcatta atcattttta aacattaaaa agagactatc tacaataatc
    20461 tcatttgcat tttatctaac taagtttaac ttttgcttag aaaaatgatg ctatgaggat
    20521 attcagtaga tagttttttt atgatctaat atttaaactt agatatcgtt ttgtaattaa
    20581 ccgagataac tcatcaccta gcaatattat ttacaaaata tttcgataaa attgtgtatt
    20641 ttgcataaat taaaaatgta tgttttaaaa aattgttata attaaattaa gattaaacaa
    20701 aggggtgact gttatgtcag aagaaaaaca tgtagttgaa catgaacaac aaaagaaaga
    20761 aaagacaaaa aagcaataca agccattttg gattgtcatg agttttataa tacttatagt
    20821 tgtactatta ctcccggcac cttcaagtct gccgataatg gctaaggcag tactagctat
    20881 tttagctttt gcagttatta tgtgggtaac ggaagctgta tcatatccgg tgtcagcaac
    20941 tttaattatt ggcttaatga tattactttt aggatttagc cctgttcaaa atttagggga
    21001 gaagctaggt aatccgaaaa gtggcagtgc tattttagct ggaagtgacc ttctaggaac
    21061 taatcatgca ttatcattag cgtttagtgg atttgcaact tcagctgtag ctctcgttgc
    21121 agctgcatta tttttggctg ctgctatgca agaaacgaat ttgcataaaa gactagctct
    21181 tttagtgtta tcaattgttg gtaataaaac tagaaatata gttattggag caattatcgt
    21241 ttcaattgta cttgcatttt tcgttccttc tgcaacagct agagcagggg cagttgtacc
    21301 aatcttgctg ggtatgattg cggcatttaa agtttccaaa gatagcaagt tagcgtcttt
    21361 attaataatt acttcagtac aagctgtgtc aatttggaat attggtatca aaacggcggc
    21421 agcacaaaat atcgtagcga ttaattttat aaaccatcaa ttaggatttg atgtttcatg
    21481 gggcgagtgg ttcttatatg cagcgccttg gtccatagtt atgtccgtag ctttatattt
    21541 catcatgatt aaagtgatgc ctccagaaat taatacaata gaaggtggta aagatttaat
    21601 aaaagaagaa ttgcataaac ttggccccgt tagcccacgt gaatggcgtt taattgttat
    21661 atcgatgtta ttattactgt tttggtcaac tgaaaaagta ttacatccga ttgactctgc
    21721 atccattact attattgctt taggtgttat gttaatgccg aaaattggtg tcatgacatg
    21781 gaaacatgtt gaaaataaaa taccatgggg aacaattatc gtgtttggtg taggtatttc
    21841 actaggtaac gttcttttga aaacaggtgc agctcaatgg ttaagtgatc aaacttttgg
    21901 tgttttaggt ttaaaacatt tacctattat cgcgacaatt gcgcttatca cgctttttaa
    21961 tatattgatt catttgggct ttgcgagtgc aacaagttta tcatcagcgt taatacctgt
    22021 ttttatttcg ctaacctcta cgttacactt aggagaccag tctataggat ttgttttaat
    22081 tcaacaattt gttattagtt ttggtttctt attacctgtt agtgcacctc aaaatatgtt
    22141 ggcttatggc actggtactt ttacggttaa agatttcttg aaggcaggta taccattgac
    22201 aattgtaggg tatattctag tgatagtttt tagcatgact tattggaaat ggttaggttt
    22261 gctttaatta aaaatataaa taagaatcta ggttatttta aagtgacaaa aagcttaata
    22321 aaataaaaag ataattgaag ggtgttttgt ttatggcaat tgctgtgtta ttaaatcgaa
    22381 tgtttcgaat ggaacacaat ccattatttg aatatattta tcaacaaaaa gaagacattg
    22441 atgcatgtta ttttatcatt ccggaagagg acatgtcttc agcttctgat ttgaaagcac
    22501 agttttatcg cggtactttg cagcgctttt accaatcgtt gcacgcagaa aagcttacac
    22561 cttatgttat gtcttatgac gatatcattt cattttgtaa agaaaacaat atctctgaag
    22621 tagtgactgc gggtgatatt atgagttatc atcttgaaga atatgatatt ttacatcaac
    22681 gttctttatt caatgaagca cgcattgccg ttactttgat acgtgggaat cattacttta
    22741 aagcgagtaa aacaatgaat caacaagggg agccatacaa tgtttttact agtttctata
    22801 aaaaatggcg accttacttg aggcatagag acgtatatca ctatgattta aaatcattcg
    22861 aagactttgt cattgcatca cctgatgatt tagtgtttga tgacatagca tttggatcct
    22921 cacaaataat tgaacagaat aaatggcaac attttttaga tcaagatata cagaattacg
    22981 aaagcggaag agactattta cctgaagtat taacaagtca gctaagtgtt gctttagcat
    23041 atggattatt agatattatt gaaattttta atgatttatt ggcgcgttat gatgaagatg
    23101 aggcaaacta tgaagcattt atacgtgaac tcatttttag agaattttat tatgtgttaa
    23161 tgacacagta tcctgaaacc tcataccaag ctttcaaacc taaatatcga cagataaaat
    23221 ggtcgcaaaa tgaagcggat tttaatgcat ggtgcgaagg gcaaacagga tttccaatca
    23281 ttgatgcagc aataatggaa ttgacacaaa ctggttttat gcataatcga atgagaatgg
    23341 ttgtgtcgca atttttaacc aaagatttat ttatagattg gacatgggga gaaaaattct
    23401 ttagaaagca ccttattgac tatgatgcag catcaaatat tcatggatgg caatggtctg
    23461 cttctacagg tacggatgca gtgccgtatt ttagaatgtt taatccaata agacagagtg
    23521 aacgctttga tgctaaagct ttgtatatca aaacatatct tccgattttt aatcaaattg
    23581 atgcaaaata tttgcatgat acacaacgca atgagtccaa cctttttgaa caggggattg
    23641 aattaggtag tcattatcca agacaaatgg tagatcatca agaaaaacgt acacaagttt
    23701 tagctacatt taaagcgcta gactaattcg gctcaattga tagatctggc atgagtaaaa
    23761 tctggagcag aatataaagt tcttaagcaa tataaataag ccaatttcta ttattgatgt
    23821 gatagaaatt ggctttcctc aaatatataa gttgtattta gttttcttat taatttgatg
    23881 ttatcttagt atgtccgtaa ataaagtgag gtatagtacg acatactcta aaaacgtagc
    23941 gagataaata tatttcaatc taacttttat gttttgaggc acttgccatt taggatattg
    24001 tcgttcgtaa tacgacactt gttgtataaa tacacctagt ccaaatggca gcatcatgag
    24061 taagatactt cttaaataac ttaaaccaat atcatgccat atgtgtccaa taatcaattg
    24121 aaagacaatg atagatacta ttaaaacgat tatatttatt gtcacttgtt caaacgcact
    24181 ccttttccaa ataatagaat tgctgcttgc atgacaacca taaaacatac aaacatagca
    24241 gttttaagcg ttagactttc tagaatgtga tttagaacat gtaagggctc attaaagaaa
    24301 taaacggaat gtaagcgtaa gaaacgacca atataaattc cgaatccatt taaaaacatt
    24361 agcacgacaa caattaatct attaagccaa cggtgagaag tcaatgttag tatttcaaaa
    24421 tagattaaaa tcatcacata aaccgctaag aagacaccaa gcagtaaata ggtaaagtat
    24481 ttccactcac ttaaatttag tcctgcgtaa aagttgaatt gaaattggtt caaatggatt
    24541 aaatcagtta ccatataaaa tgtatttggt aaaagtaaca caaatataaa actatatatg
    24601 ataaagagtg gccattcata ctttttattc ggtttgaata atcgtaataa tagactaagc
    24661 tcaaaaggta tatatgctaa aaacaaattt aaagtcataa attgaaaaat tttagtctca
    24721 aaaagggaga cgataaataa aattaaaaag taaattctag cgatgtatcg agattgcatc
    24781 aataataaac actactttct aatgaaaaag ttattcattc aaatgtgata gttataaacg
    24841 cattcaattc ctaattaaaa atttaattta actcgttgaa gtatattaat aaacgagaat
    24901 gcatttatga ttaagcactt atgataactt gtaataaaat ttgattcaac taaaaagtaa
    24961 agtgctattg aggatcaagt aaatttaaac cgactgcatc tttgaatgaa cgttgaatgt
    25021 ccaaatcagt tacagtaaga atgatttcag cagttgcaga aacaattgct ttagcgagat
    25081 gtccgctaaa agtttcatat gattgagtac cgaaagtagc atgcaaatgt gcaaaatgac
    25141 cattgtctag acgagaaata ttacctaata agctcgtcaa ttccagtggc tcagtaatat
    25201 gtttttcttc gtattgtttc gttgttaaat tgaaaaattt taatacaacg tcatcacatg
    25261 caccaatgcc gctgacagat gtaaatgtta agtcttggtc atctgcaaag gttgttatac
    25321 attcaacgat atcttctcct ttttccaaca ctagtagtat agtatgatta cttttttgca
    25381 atttcatatg atcaatcccc tttattttaa tatgtcatta attatacaat taaatggaaa
    25441 atagtgataa ttacaaagaa aaaatattgt caaatgtagc aatgttgtaa tacaatatag
    25501 aaacttttta cgaatattta gcatgaattg caatctgtcg tggaaaagaa gaataacagc
    25561 tttaagcatg acatggagaa aaaagaggtg agcatatgaa taaacagatt tttgtcttat
    25621 attttaatat tttcttgatt tttttaggta tcggtttagt aataccagtc ttgcctgttt
    25681 atttaaaaga tttgggatta actggtagtg atttaggatt actagttgct gcttttgcgt
    25741 tatctcaaat gattatatcg ccgtttggtg gtacgctagc tgacaaatta gggaagaaat
    25801 taattatatg tataggatta attttgtttt cagtgtcaga atttatgttt gcagttggcc
    25861 acaatttttc ggtattgatg ttatcgagag tgattggtgg tatgagtgct ggtatggtaa
    25921 tgcctggtgt gacaggttta atagctgaca tttcaccaag ccatcaaaaa gcaaaaaact
    25981 ttggctacat gtcagcgatt atcaattctg gattcatttt aggaccaggg attggtggat
    26041 ttatggcaga agtttcacat cgtatgccat tttactttgc aggagcatta ggtattctag
    26101 catttataat gtcaattgta ttgattcacg atccgaaaaa gtctacgaca agtggtttcc
    26161 aaaagttaga gccacaattg ctaacgaaaa ttaactggaa agtgtttatt acaccagtta
    26221 ttttaacact tgtattatcg tttggtttat ctgcatttga aacattgtat tcactataca
    26281 cagctgacaa ggtaaattat tcacctaaag atatttcgat tgctattacg ggtggcggta
    26341 tatttggggc acttttccaa atctatttct tcgataaatt tatgaagtat ttctcagagt
    26401 taacatttat agcttggtca ttattatatt cagttgttgt cttaatatta ttagtttttg
    26461 ctaatgacta ttggtcaata atgttaatca gttttgttgt cttcataggt tttgatatga
    26521 tacgaccagc cattacaaat tatttttcta atattgctgg agaaaggcaa ggctttgcag
    26581 gcggattgaa ctcgacattc actagtatgg gtaatttcat aggtccttta atcgcaggtg
    26641 cgttatttga tgtacacatt gaagcaccaa tttatatggc tataggtgtt tcattagcag
    26701 gtgttgttat tgttttaatt gaaaagcaac atagagcaaa attgaaagaa caaaatatgt
    26761 agcataagta ttttggtgta tattgatata aagtaaagcg taatattatg aatgattagc
    26821 atcgtttttc ttatgaattt tattaagaaa attcgatgct ttacatttaa aaagattcga
    26881 ttgactaaat gttttactct ttatatttaa atgttatatg taacaaaaaa tgattttgag
    26941 taataaacat gttacaaata ttacattctt tttaaattgc aatccacata cctaattcat
    27001 taacgttaat gtgttaagat gataaaaaat gagtaaggaa atgtgggtaa ggggatgaca
    27061 gtaaaaaatt tatttttagg ctttgttgct gtaatattaa ccgtttgttt aattggttta
    27121 ttaatattag caacaaatga agatgcgctt gctaaggtac ataaaacaat taatacgctt
    27181 aacgcgataa atgtatcaac tgaagatact tataaaaaga aaatggatat tctcaatatt
    27241 catactgcta aagcatctga agtgaatgaa aatgtgaaaa agcaaaatca ttttaaacat
    27301 cgtgtgaatg caaataaatc aaattctttt aacgaacaag agtgccaagt tattgctgat
    27361 cgttatgcag ataagcatat caatgataat tatggtttag aaagaatttc taagacaaat
    27421 catggatata attatgtgta ttccaatgat aattcaacta gtaagcaaca tgtaagtatt
    27481 tcaaatcaag gcataataac gaaataatag atggaacagt gtattctaat tggatatact
    27541 gtttttattt tgcaataatt taatttaaaa aggtgaattc aacttataaa atgatgtaaa
    27601 tgttatgtca aaatcaacca atccgtaatg tattttaaaa tgttaatata gttctgaaga
    27661 agtataaatg aggtgttgaa atggctaaaa ataagaaaac gaacgcgatg cgtatgcttg
    27721 atcgtgcaaa aattaaatac gaagttcata gctttgaggt accagaagaa catttatctg
    27781 gtcaagaagt cgcagaactc atacaagcaa atgttaaaac agtatttaaa acgcttgttc
    27841 tagaaaatac aaaacatgaa cattttgtat ttgttatccc agtaagtgaa actttagata
    27901 tgaaaaaggc agctgctttg gttggagaga agaaattgca gcttatgcct ttagataatt
    27961 tgaaaaatgt aacgggatac attcgtggtg ggtgttcgcc tgttggtatg aaaacattgt
    28021 ttccaacagt cgttgacaaa tcgtgtgaaa attatagtca tatcagtgtg agtggtgggc
    28081 ttcgaacaat gcaaatcaca atagctgttg aggatttgat tacaataact aaaggcaaaa
    28141 ttggagcagt tatccatgaa tgattaataa caacaaaaag tatggggcaa gattaggagt
    28201 gtgacagaga tgaaattttt ataaaattca tttctgccac actccttttt gattgaatta
    28261 gcattttacg atcataaaca gtcattataa ttgagtattt gaacataaaa atgtaatttt
    28321 atcgtcacaa tttgagtgtt tgtgattgtt tttggtaatt tatgattgaa aagtgaaagc
    28381 gtactcatta taatacaaag tgagatgggg tgatgatgat aattactgaa aaaagacacg
    28441 agttaatatt agaagaactt tcgcacaaag attttttgac tttacaagaa ttaatagatc
    28501 gaactggttg cagtgcttca acaatacgaa gagatttatc taaactacaa caattaggga
    28561 aactgcaacg tgtgcatggt ggtgcaatgt taaaagaaaa tcgtatggtt gaggcgaatt
    28621 taactgaaaa attagcaacg aatcttgatg aaaagaaaat gattgctaaa atagcagcta
    28681 atcaaatcaa cgataatgaa tgcttattta tcgatgctgg ttcatctaca ttggagctaa
    28741 ttaaatatat tcaagcgaaa gatatcattg tggtaactaa tggtttaaca catgtagaag
    28801 ctttacttaa aaaaggtatt aaaacaatta tgctaggtgg tcaagttaaa gaaaatacac
    28861 ttgctacgat tggttctagt gctatggaga tattaagacg atattgtttc gataaagctt
    28921 ttatcgggat gaatggatta gatattgaac ttggattaac tactcccgat gagcaagagg
    28981 cattagttaa acaaacagca atgtcattag ccaatcaatc atttgtactt atagatcatt
    29041 ctaagtttaa taaagtatat tttgctcgtg tacctttgct agaaagtacg acaatcatca
    29101 catctgaaaa agcattaaat caagaatcgt taaaagaata ccaacaaaag tatcacttta
    29161 taggagggac tttatgattt atacagtgac tttcaatcct tcaattgact atgtcatttt
    29221 tacgaatgat tttaaaattg atggtttgaa cagagcaaca gcaacatata aattcgctgg
    29281 ggggaaaggt attaatgtct cgcgcgtctt aaagacattg gatgttgagt caactgcctt
    29341 gggatttgca ggtggatttc ctgggaaatt cattatagat acattaaata acagtgcaat
    29401 tcaatcgaat tttattgaag ttgatgaaga tacacgtatt aatgtgaaat taaaaacagg
    29461 acaagaaaca gaaatcaatg caccgggtcc tcatataacg tcaacacaat ttgaacaact
    29521 gttacaacaa attaaaaata caacaagcga agatatagtt attgttgctg gaagtgtacc
    29581 aagtagtatt ccaagcgatg cgtatgcgca aattgcacaa attacagcac agacaggtgc
    29641 taaattagta gtcgacgctg aaaaagaatt ggctgaaagc gttttaccat atcatccact
    29701 atttattaaa cctaataaag atgaattaga agtgatgttt aatacaacag tgaactcaga
    29761 cacagatgtt attaaatatg gtcgtttgtt agttgataaa ggtgcgcaat ctgttattgt
    29821 ctcgcttggc ggtgatggtg ctatttatat tgataaagaa atcagtatta aagcagttaa
    29881 tccacaaggg aaagtggtta atacagttgg ctctggtgat agtacagttg caggcatggt
    29941 ggctggaatt gcttcaggtt taacgattga aaaagcattc caacaagcag tcgcatgcgg
    30001 tactgccacg gcatttgatg aggacttagc aacacgggac gctatagaaa aaataaaatc
    30061 acaagttacg attagcgtac ttgatgggga gtgaaaataa tgagagtaac agagttatta
    30121 acaaaagata caatagcaat ggatttaatg gcaaatgaca aaaatggtgt tattgatgag
    30181 ttagtaaatc aattagacaa agcaggtaaa ttaagtgatg tcgcgtcatt taaggaagcg
    30241 attcacaatc gagaatcaca aagtacaact ggtatcggcg aaggtattgc cattccacat
    30301 gccaaagtgg ccgcagttaa gtcaccagct attgcgtttg gtaaatctaa agcaggcgta
    30361 gattatcaaa gtttggatat gcaaccagca cacttattct ttatgattgc agcgccagaa
    30421 ggtggcgccc aaacacatct agatgcttta gctaagttgt ctggtatttt aatggatgaa
    30481 aatgtacgtg agaaattatt acatgcttca tcacctgaag aagtactagc gatcatagat
    30541 gaggctgatg atgaagtgac aaaagaagaa gaggcagaag ctgaagcaca acaagttgca
    30601 actgcagaac aatcatctaa acaatctaat gagccatatg tgttagcagt aactgcttgt
    30661 ccaacaggta ttgcacacac atatatggca cgtgatgcat tgaaaaagca agcggataaa
    30721 atgggtatta aaattaaagt agaaacgaat ggttcaagcg gcattaaaaa ccatttaact
    30781 gaacaagata ttgaaaatgc aacaggtatc attgttgctg ctgatgttca tgttgagacg
    30841 gatcgcttcg atggtaaaaa tgtcgtagaa gtaccagtag cagatggtat taaacgccca
    30901 gaagaattaa ttaataaagc attagataca agtcgtaaac cttttgttgc ccgtgatggt
    30961 caaagaaaag gtaactcaaa tgacagtcaa gaaaaattaa gcccaggtaa agcattctat
    31021 aaacacttaa tgaacggtgt ttctaacatg ttgccacttg taatatctgg tggtatttta
    31081 atggcaattg tatttttatt tggagcaaat tcatttaatc caaaaagctc agagtacaat
    31141 gcgtttgcag agcagctttg gaacattggt agtaaaagtg cattcgcgtt aatcattcca
    31201 attttatctg gattcattgc acgtagtatt gcggataaac ctggtttcgc ttcaggtctt
    31261 gtaggtggta tgttagcaat ttcaggtggt tcaggattta ttggtggtat tattgcaggt
    31321 ttcttagcag gttacttaac acaaggtgtt aaagccatga cacgtaagtt accacaagca
    31381 ttagagggat taaagccaac attaatttat ccactattaa cagtgacggc tacaggctta
    31441 ttgatgattt atgcctttaa tccaccagca tcttggttaa atcatttgtt attagatgga
    31501 ttaaacaatt tatcaggttc taatattgta ttattaggtt tagttattgg cgctatgatg
    31561 gcgattgata tgggcggtcc attcaacaaa gcggcatatg tttttgcaac aggtgcgttg
    31621 attgaaggta atgcagcacc aattacagct gcaatgattg gtggtatgat tccaccgtta
    31681 gcaattgcga cagcgatgtt aatttttaga cgtaaattta caaaagaaca acgtggttca
    31741 attatcccta actatgtgat gggtatgtca tttattacag aaggtgcgat tccatttgca
    31801 gctgccgatc cattacgtgt tattccttca atgatgattg gttcaggtat aggtggcgca
    31861 attgctttag gcttaggttc acgaattact gcgccacatg gtggtattat tgtaattgtt
    31921 ggtactgatg gtgcacactt acttcaaact cttattgcac ttctagttgg cacattagtt
    31981 tcagcattaa tttacggttt aatcaaacca aagttaactg aaacagaaat cgaagcttca
    32041 aaatcaatgg acgagtagtt ttaatgatgt aaaatgattg ttagcaaaga gcttcatatt
    32101 aagttgtatg ttcaatgaat atatgttagt tttatatatc gtgttaacgg tagcttatac
    32161 aaagctgtaa aaacactttc tattaattca gtttttatga attgatatga aagtgttttt
    32221 atttttagat aaatgaatga agaaatagac accacaaatg tatagacttt tttaatattt
    32281 tgcaaaaagt tatgccaaac gaagcagata tagtaaaata tgagtgtctt aaagtgaaaa
    32341 tttataaata aagaagggtt tatacgtgtc agaattaatt atatataacg gcaaagttta
    32401 tactgaagat ggcaaaatcg ataatggtta cattcatgtg aaagatggac agattgttgc
    32461 aattggagaa gtggatgata aagcagcaat tgataatgat acgacaaata aaattcaagt
    32521 gattgatgct aaaggtcatc atgtattacc aggttttatt gatatacata ttcatggtgg
    32581 ttatggtcaa gatgcaatgg atgggtcata cgatggctta aaatatctat ccgaaaattt
    32641 gttgtctgaa gggacgacat catacttggc cactacaatg acgcaatcga ctgataaaat
    32701 agataatgca cttacaaata ttgctaaata tgaagcggag caagatgttc acaatgcagc
    32761 ggaaattgta ggtatacatt tagaaggacc atttatatct gaaaataaag ttggtgctca
    32821 acatccgcaa tacgttgtac gcccatttat cgataaaatt aaacattttc aagagactgc
    32881 taacggatta ataaagatta tgacgtttgc acctgaagtt gaaggtgcaa aagaagcgct
    32941 tgaaacgtat aaagatgaca ttattttttc aattggtcat acagtagcaa catacgaaga
    33001 agcagttgaa gctgttgagc gaggagctaa acatgtcacg catttatata atgcagcgac
    33061 gccattccaa catagagaac caggtgtttt tggagcagca tggttgaatg atgctctaca
    33121 taccgaaatg attgttgatg gcactcattc tcatccggca tcggttgcaa ttgcttaccg
    33181 tatgaaaggt aatgaacgtt tttatttaat taccgatgca atgcgtgcaa aaggtatgcc
    33241 tgaaggagaa tatgatttgg gtggacaaaa agtaactgtt caatcgcaac aagcacgtct
    33301 tgcaaatggt gcgcttgctg gtagtatttt aaaaatgaat catgggttac gtaacttaat
    33361 atcatttaca ggtgatacat tagatcattt atggcgagta acaagtttaa atcaagccat
    33421 tgcattaggt atcgatgata gaaaaggtag tattaaagta aataaggatg cagatcttgt
    33481 tattctagat gatgatatga atgtaaaatc tacaataaaa caaggcaagg ttcacacatt
    33541 tagctaataa ataatcataa ttaaatgtat gcaatagatt taatctgtta acataagcac
    33601 tttatattat gataaaatag aagcaataac atttttttct gggggtgtct aaatgggaag
    33661 gcgataacat gtagttgtaa tttaagtcat agtgataaat ttgaatgcgt gttacccatg
    33721 agtgacacat ataacatgga ggtgaatccc tagaaatagg gaattaattg gaaacttcga
    33781 ccataattag tttgattata tttattctat taattgcatt aaccactgta tttgttggtt
    33841 cagaatttgc attagtaaaa attagagcaa caagaattga acagctagca gatgaaggaa
    33901 ataaacctgc taaaatagta aaaaagatga ttgctaatct agattattat ctttctgctt
    33961 gtcagttagg tataacagta acatctttag ggttaggttg gcttggtgaa ccaacgtttg
    34021 aaaagctatt acacccaata tttgaagcaa tcaatttacc aactgcatta acgacgacga
    34081 tttcgtttgc agtgtcattt ataatcgtta cgtatttgca tgtagtactt ggtgaattag
    34141 cgcctaaatc tatagctatt caacatactg aaaagcttgc tttagtatat gcaagaccat
    34201 tgttctattt cggtaacatt atgaaaccat tgatttggct gatgaatggt tctgcacgtg
    34261 ttattattag aatgtttggt gtaaatcctg atgcccaaac tgatgcaatg tcagaagaag
    34321 aaatcaaaat tattattaac aatagttata atggtggaga aatcaaccaa actgaattgg
    34381 catatatgca aaatatcttt tcattcgatg aaagacatgc aaaagatata atggtaccta
    34441 gaactcaaat gattacacta aatgaacctt ttaatgtaga cgaattacta gaaacaataa
    34501 aagaacatca atttacgcgt tatccaatta ctgatgatgg tgataaagac cacattaaag
    34561 gatttattaa cgtcaaagaa tttttaactg aatacgcttc tggaaaaacg attaaaatag
    34621 caaactatat acatgagttg ccaatgattt cagagacaac acgtatcagt gatgcattaa
    34681 ttagaatgca acgtgaacat gtacatatga gtcttattat agatgaatat ggtggaacgg
    34741 caggtatttt aacgatggaa gatattttag aagaaatcgt tggagaaatt cgtgatgaat
    34801 ttgatgatga tgaagtgaat gatatcgtta aaattgataa taagacattc caagtaaatg
    34861 gcagagtact attggatgat ttaactgaag agttcggtat agaatttgat gactctgagg
    34921 atattgatac gataggtgga tggttacaat ctcgtaatac caatttacaa aaagatgatt
    34981 acgtggatac aacttatgat cgctgggttg tttcagaaat cgataaccac caaattattt
    35041 gggtgatatt aaactatgaa tttaatgaag cgagacctac tatcggacag tctgatgaag
    35101 atgaaaaatc agaatagata ttaatatata aaccaactaa gaatgattta attcattttt
    35161 ggttggttat ttttttgact aaaattaatg aaaagtgaaa atagtattgg aactcaatat
    35221 ctttaatgat ttaatgaata atttttattg aaagcgataa ttcgtattaa ttgagtttgt
    35281 tgaaaaattt agggtaatgt aaagatataa aagatacata gattggagag atataaagat
    35341 gttgaatgag atacaaatat taaataatgg atacccgatg ccttcagttg ggttaggtgt
    35401 ttataaaatc tctgacgaag atatgactaa agttgtaaat gctgcaattg acgcaggcta
    35461 tagagcgttt gatacagcat acttttatga taatgaggct tcactaggac gagcattaaa
    35521 ggataatggc gtcgatagag aagatttgtt tataacaacg aagttatgga atgactatca
    35581 aggttatgag aaaacattcg aatatttcaa caaatcgatt gaaaatttac aaactgatta
    35641 tcttgattta tttctaatac attggccttg tgaagcagat ggtctatttt tagaaacata
    35701 taaagctatg gaagaacttt acgagcaagg taaggtaaaa gcaataggtg tatgtaattt
    35761 taatgttcat catctagaaa aattaatggc tcaatcaagt atcaaaccaa tggtgaatca
    35821 aattgaggta catccatatt ttaaccaaca agaattacaa gaattttgtg atcgtcacga
    35881 tattaaagtg actgcatgga tgcctttgat gagaaataga ggactactag acgaccctgt
    35941 cattgttaaa attgctgaaa aatatcataa aacaccagca caagttgtat tacgttggca
    36001 tttagcacac aatagaatta ttattccaaa atctcagaca cctaaacgca ttcaagaaaa
    36061 tatagatatt ttagatttta atttagaatt aacagaagta gctgaaattg atgctttaaa
    36121 tagaaatgca agacaaggta aaaatccaga tgatgtgaaa attggggatt taaaataact
    36181 ggatgttaaa ttttacgttt atgaatgcct tttaatgtgt acattaaaat aaatgagttg
    36241 gtttttacta tttgataaaa caatactcag gtacattcaa aatcttttaa ataaaaagga
    36301 tggacataga tgaaaattag agtcgtcatt ccttgtttta atgaagggga agtcattaca
    36361 caaacacatc aacaattaac tgaaatactt tcacaagata gtagtgtgaa aggctatgat
    36421 tataatatgc ttttcataga tgatggtagt acggatacca ctatagatga aatgcaacat
    36481 cttgccacaa tagataggca tgtcagcttt atttctttta gtagaaattt tggaaaagaa
    36541 gcagctatga ttgcaggtta ccagcatagt actgaatttg atgcagtcat catgatagat
    36601 tgtgatttgc aacatccacc tgaatatatt ccgaaaatgg ttgaaggttt tatggaaggc
    36661 tatgatcaag tgattgcaaa gcgtgataga agtggtgaaa attttagtcg caaaacatta
    36721 agccatttgt attataagtt agttaattgc tttgtagaag aagtacaatt tgatgatggt
    36781 gttggtgatt ttagactttt aagccaaaga gctgttaaat ccattgcatc acttgaagaa
    36841 tataatcgat tttcaaaagg gttatttgaa tggataggct ataatactaa agtgtttacg
    36901 tatcaaaatg ttgagagaca aaaaggggaa tctaagtggt cctttaaaaa gttatttaat
    36961 tatggtattg atggattgat ttcctttaat agtaaacctt tgagaatgat gatttatctt
    37021 ggcttgttta tcttttcaat aagcgtgtta tatattatct atttattcat caatattatg
    37081 atatctggtg ttaatattcc gggatatttt tcaacgattg cagctatttt attattaggc
    37141 ggcatacagt taatttcaat tggtgttgta ggtgaatata ttggcaggat atattatgaa
    37201 gttaaggcac gtcctaaata tattattcaa gctacaaatc tttcaagtct tgaaaatgat
    37261 gagaaggata cccataaagt ttattctaaa taaacaaaaa aagaagccct cattaatggg
    37321 agcttctttt tagtctttgc attttatttt ataaataaat cggattatga cgtaatgtct
    37381 aatttgtgta atgttacagt catcgtagtt cctacatcta tatcactgct tacactgatt
    37441 tttgcgttat tttgttgcgc gagttcatta gctatatata agcctaatcc agaaccaccc
    37501 gtttttgtat tacgagagtt ttctactctg aatgtacgtt cgaatatacg ttcttgtagt
    37561 tctggtataa tgccaatacc ttcatcgcta atagcaatgt cgatagtatc ttgatcttcg
    37621 ttttcactaa tattaatatc aatgcgacta ccaacatttg aaaattttag cgcattatca
    37681 agtaagtttg ttaaaatacg ctcaagtggc gttcgatatt gataaaatgc atcaatttcg
    37741 ttacagaaat tcacttctaa tgtgcggttt tcatgtttga tacgttgctc atatggttgc
    37801 aatattgata caagtaattg gtctagttgt attaattctg ggggatatgt tttacctgta
    37861 tttaaagtga taatatgagt catatcatca aataatgttg ataatctgtt tgcttgttta
    37921 attaatatgt cgtatgactc tttaatctca tgatccttag tgattatacc atcacgtagt
    37981 ccttcagaat atgaaataat gcttgctaaa ggtgttttta aatcatgggc taagttttga
    38041 atcagttctg ttttttcttg ttgttcggat ttaatttgat tcatttgttg cgtaatttca
    38101 gaagccattt tattaaaaga ttgatttaat tcataaattt cttttggtga attaaacgtt
    38161 ttatcattgc ttgcgtaatt tccgttagca aattgcttag tttttatatt aaactgctta
    38221 attttttgta taagtggatt aataaaaata ctacatatta ataaggttaa acagcttgta
    38281 attattgtcg ttaaggtcaa agttagtgtc atatggccgt taaaccacat taaaatatat
    38341 gcaattgcta aaatagttga agttaatagt atactcgata cgacgccaat aatgatttga
    38401 cttctaattg ataacaccat tatcggctcc tttcaaattt atatcctaat ccccatacag
    38461 ttgtgatggt atatgttgta aagctctctt tttctaattt ttctctaata cggtgtatat
    38521 ggacattcac ggtattagca tcttcgtaat agtcatatcc ccaaactttt tcaagtaatt
    38581 ctgatttaga aataacttca ttttctctag aagctaaata ccacaataac tcaaattcct
    38641 taatacgcat agggacttcg tgaccattta cagtcacaac tttacttaag ttaataagtg
    38701 ttaattcatc aaacgacagt tgttcaactg gttgatgatg gtatttcttc attcttgtaa
    38761 gtaaattatt aatacgtaaa acgagttccc ttggactaaa tggttttttg acatagtcat
    38821 ctgcacctaa agttaaggcg taaatggtat catgttcttg tgttttggca gttaaataga
    38881 taaaggggat atctaatttt tgccttttca tttctttgac aatgtcgtaa ccattaactt
    38941 ctggcatcat gatatcaagt accatgatat caatatcatt tgatagtaaa gaaattgctt
    39001 ctttaccgct agttgtcgtt gttactttgt aaccttcata ttcaaaatag gtttgacaaa
    39061 tgtctacaat gtcttgttca tcatccacga tcagtaagtg ggtcatctat tttttcacct
    39121 ctgttcttac gacctctaaa gtaattaatg atttctttaa gtgaaatctg ttttaacaat
    39181 gagtgactca ttagtaaaag gataaagaaa gttaattgaa gaggatacgt aaatatcata
    39241 tctgctaaga tataatttat cataacaaag gctccaaaga aactagcagc atatgcaaaa
    39301 actccaaaaa ttaaacctaa tccaattgca atctctccga gtgggacaac aatatcaaat
    39361 aatgacgtcg tatgtgcaac tatatttgcg aaaaaccact tataccactc tggtgaatca
    39421 gtattgttag cgatgactgg tactaaacct ttcagcgtaa atccgcccgt taatttttcg
    39481 tagccttgca ttaacataac aatacctgaa cccacacgaa tgataaatgt aacgagtagc
    39541 aataatttat tcatgatgtt cacattcttt ctatttattg tgtgtaattt atataaacat
    39601 aagattaagc aacataatgc gatttgtagt gttatgtgaa caggaagtgt ttgagctcat
    39661 gggttgctta agctaaacaa acctcaagct ctaaaaaaat ttacttatgt gctctgcaat
    39721 cttatgggat ttaatagttg tatatatatt gaaaaaagga aagtatgatt tctacaagct
    39781 aggggggctg tgaaatcata cgctttccat tgagtaatga gtgtaatgta tattttaaaa
    39841 atttgtaatg tatagtaata gtatgttgta aataaaagtt taactcagaa attgattatt
    39901 ttaatttagc gccgccgaag atgacgtgag cttttttaca atagattgtt acttcatcat
    39961 atttgcttaa atctacattt ttaagatcaa atgtttgttt ttctttatcg tagtcaacca
    40021 ttgcgatttc tttaccgttt ttaatgtcgc cattttttgt taggtagacg tataaatctg
    40081 gaccttttga tgatttgtag ttagtaagca ttaatttacc atttttaatc tcagctttac
    40141 cttcaacagt ttcaccgttt ttagaactga atgtacctgt taggtgtttt gttttatcag
    40201 ttttaacatt gctatcttct gactttgttt tttgttcagt tttgttacct tgatcttgtg
    40261 aattagaatt accacaagca cctaaagcta atgttgtgat aacagcacca gctgctaaaa
    40321 aatattttgt attcatgcta actcctcatt tcttcaattt gataagttaa gtttaaaatg
    40381 aaggcaaata atatgccatt aactaattct taacttcgtt taaatatcgc ttaactaata
    40441 ttgaagaaag aataatttga aaatgaaaat aattgtctga tttaaataaa taggggtcaa
    40501 aataagagga gggcgttttt ttaagtaagg aaatatttat ctgaactaga taacactata
    40561 aaacacttct gatattttga actaagaata tcagaagtgt ttaatagttt tggtattaaa
    40621 atttataaac cttttgccac aataaaaaca acaagaaaat aagatagacg attaaataca
    40681 agtaccaata tggtgttaaa accatcataa cgataaatga aatgttaata atgataagtc
    40741 ctttaatcat tagtttcttt attttgttat tattttcagc agtttcatct aagctattaa
    40801 atgttgaaat aaacaacata ataccaccaa tgatgaaaat ggcaggggct aaaaatggaa
    40861 tagacatata tattgattgt gtacttgaaa agtcggtaac acgttgcgtt aaactgacaa
    40921 caatcgttaa tatgatagca ataaattttt ggtctgtaat gtatacaggt tttaattttt
    40981 tatcaatgta aaactgtagt atcgtccaaa tgataaacat cgttatagta cacagagtaa
    41041 taaagttata tttatcaatt atgaatgtat ttataaaggc gtttatcgta atagataaca
    41101 acattgcact taatgataaa tgttgcattt gatgcctata taaattgaat ccagttataa
    41161 gtacgacgat agcaatatta attaatatat atacaatcat gatggactcc tgttcgttat
    41221 acttcaatca actattatat caattaatat attgttattc attagatatg acttgcaaaa
    41281 agtaaaataa gttatccttt atacaccttt tttattgctc caaagtaatg tatgaagttg
    41341 tggtaacaca taaacgtgat tcatatcatt actttgcata actaaatcca ccaactgctc
    41401 gtagcgttct aacaactttt cggtatgatt atctacgctg tctgataaat atgggttacc
    41461 aacttgtaaa tagaagggaa tatctggata acggtggtgt atcattttgg caaaatcata
    41521 atctttatcg tcgaatacaa ctacttttaa gtttaatgat gaaggtacgc attgtgtaat
    41581 cacttcatct aactttttta aatcaggtgt catagttgaa cttggtggtt ttggactaat
    41641 cgttaaatca tcaatttgtg tcatccaagg ttggaattta ctgccttgtg tctccagtgc
    41701 gctgaaaata cctttatctt gaaataagtc aactaactct tggatacctt taattaatgc
    41761 tgggttacca ccagaaattg taacgtggtt aaataaatcg ccaccaattc gttttaattc
    41821 atcataaatt tcttcagcgg tcatgagttt tatatcgcct ttagcactac catcccaagt
    41881 aaatgcagaa tcacaccagc tacagcgata atcacatcca gctgttctca caaacatcgt
    41941 ttttctaccg attactcgac cttcaccctg aatggttgga ccgaatattt cgagtacagg
    42001 aattttagcc attagttaca cctgttcctt tggtctaaat acgacataac ttgttggtgt
    42061 ttctcttaca aatacttgaa tacattttgg ttggtgttcg agcgatgcca aattttcttt
    42121 aacaatttga taaattgttt ccgctacgat ttcagttgaa gggattttgt ttttaaaagc
    42181 aggtaagtta tttaacagtt gatggtcaaa tttaccgtgt atcatctttt tcaaatggct
    42241 aaagttcact aagaagccag tgtcatctag tttatcaccg acaattgtta aattaacaaa
    42301 gtaagtatga ccatggacat tttgacaaat acctgcttct tcacatggaa tgtgatgtgc
    42361 agccgaaaaa ttaaaatctt tatttaattc gaattgatat ggatgcgttg tactaggata
    42421 gatttgttgt aacattttaa agcgctcctt tactttcaag atattgattt agtccacgtt
    42481 gacgtaaatg acaagctgga cattcaccac agccatcccc aatgataccg ttatagcatg
    42541 ttaatgtttt tgtacgaata taatctaaaa cttcgagttc atcacttaat ttccacgttt
    42601 ctgctttgtt taaccacatt aaaggagtat gaatgacaaa atctttgtcc atagctaggc
    42661 ttaatgttac gttcattgat tttataaaac tatcgcgaca gtctgggtag cctgaaaagt
    42721 ctgtttcaca tacgcctgta ataatatgct tagccccaat ttgataagct agagcgcctg
    42781 caaacgacaa gaaaagtaaa tttctagctg gaacaaatgt attaggtata ccatcttcat
    42841 tattagtaat ttccatatca tgttgtgtta atgcgtttgg agtaagttgt gataataatg
    42901 acatatctaa aacgtgatgt ttcattcctt gatcttgtgc aatttgtttt gcgacttcaa
    42961 tttcagtatc atgtctttgg ccataattaa acgttacgag ttcaacttct ttgaaatgtt
    43021 tttttgcata aaagagacat gttgtactgt cttgaccacc actaaagaca acgatggctt
    43081 tttcattatt taatacactt tccattttgt aattgctcct atcattaata atattaataa
    43141 agaggttaat ggcattgata agcccgtttt taatttataa aataaaaaaa gcctatctcc
    43201 ataaaagata gacgaaagaa atgggttgct cctataatat atattaaagg tttaccaacg
    43261 aatgtttgaa atttcaatgt ctagtttttt atagagggtt tcagctagga acctctgata
    43321 ttcatttatg tacgatgaat agaatatacc atatattagt taaaatcaag gtcaggataa
    43381 tgacaatgct tttaaagttt tagctacatc caatacatat tataaaaata aaacacattt
    43441 agaacggagt atacaatgat tctagtcata gataataatg attcatttac atataattta
    43501 atagactata ttaagactca aacgaaacta acagttcaag ttgttggtat tgataatctg
    43561 ctgatagaag acgtcattaa tatgaagcca aaagcaattg ttatttcgcc tgggccgggt
    43621 aatccggatg attatcctat cttgaatgaa gtgttagaac aattttatca gcgtgtacct
    43681 atactaggtg tatgtttagg atttcaatgt atcgtgtctt attttggtgg aaatatcatt
    43741 cacggctatc atcctgtaca cggacatact acacagttac gccataccaa tgaaggtatt
    43801 tttcaaggac tgcctcaaaa tttcaatgta atgcgttatc attcattaat tgctgacgga
    43861 gcgacttttc caaattgctt aaagattaca gcaaaaaacg atgaagcgat tattatggca
    43921 tttgagcata ttagatttcc ggtttttggt gtgcaatatc atcctgaatc tattttgagt
    43981 gaatacggtt atcgacaagt tgaattattt ttatcgaagg taggtgatta ctgtgagaat
    44041 agaatataat tatcgctact atttaactga aaatgaatat aagcaatacc atattcaatt
    44101 aaagggattt ataaagaagt atgttgctac taagttggct gatgtgggag aagtgataca
    44161 ctttgcacaa gcgcagcaac gacaaggtag atatgtctcg ttatatttaa gttacgaagc
    44221 ggcaaagtat tttaatcatg ctatgtgtac acattcatta gctaaagatg atatttatgc
    44281 agcagcttat agttttgaaa aagcggaaag cataaattca acatatgaac atcaaacttc
    44341 ttatgtatca aagtatcatt tttcatttgt tgaatcttct gaggttatga tgactaatat
    44401 taaacgtgtc caacaagcaa ttgttgaagg cgaaacgtat caagtgaact atacggcgcg
    44461 cttaacggat aacatttatt atcctattag tactttatat gaacgattaa ctcaatttag
    44521 taatggtaat tatactgcgt tattacaaac tgatgaaatc caagtagcgt ctatctcacc
    44581 agaattattt tttcaaaaag gacaatttaa caatgtcgat aacgttatca taagcaaacc
    44641 gatgaaaggg acaatgccta gaggtaaaac ggaagctgaa gatcaacagt attataaaac
    44701 attgcaaact tcttcgaaag atcgtgcaga aaatgtcatg attgttgatt tactaagaaa
    44761 cgatataggg agaatatcac agagtggctc aattaaggtg tataaactat tttttattga
    44821 ggcatataaa actgtatttc aaatgacttc gatggtaagt ggaactttaa aaaataatac
    44881 agacttaact caaattttaa catcgttatt tccttgtggt tcgattacag gtgcaccgaa
    44941 actgaataca atgaaatata ttaaacaatt agaaagttca cctcgtggta tatactgcgg
    45001 agcaattgga ctattacttc caactgaaga tgataaaatg atttttaata ttccgattcg
    45061 cactattgag tataaatatg gacaagcgat ttatggagtc ggagcaggta ttacaattga
    45121 ttctaagcca aaagatgaag tgaatgaatt ttacgcaaaa accaagattt tggagatgtt
    45181 ataatgcaat tatttgaaac aatgaaaatt gataatggac atatccctag acttacttat
    45241 catactaatc gcataaaatg ttcttctgag cgattaaact ttaaatttga tgaacatgca
    45301 tggcgaaatg aattaaacga tgtaacaaca aagtatcaca gtggtcaata tagacttaaa
    45361 atcgtattaa atgctgaaag caaatttgaa acgatagtgt cacctttacc tgagaaaagt
    45421 agttttacag caaaatttca agtgttgccc aaagtagtta atccaacttt tataaaaaat
    45481 aaaacgacag aacgaaagca tttagcacac aatcatgaaa cagatttaat attgctaact
    45541 tcagaggacg gcaaggtcct tgaatttgat attggcaaca ttgtcattga agaggatgga
    45601 aaatggtaca caccaagtta taaagatgat ttcttaaaag gatgcatgcg tgattattta
    45661 atagatagtg acaaacttgt tgaaaaagac tttaataaaa acgaattgat ttataaatat
    45721 cataacaatg agatacgttt atttttgata aatagtttac gagaggttgc cgatgtccac
    45781 ctttgccttt aaaattagta tgataataaa gtagaaaatg atgtgaaaac aaatttaaaa
    45841 attaaatggg gccaaatatg attattgttt atattgtgct gttgttaatt cttgtatacg
    45901 taaattatcg attagtgaat cgattgctat ctgaaaatag aatatatgtt gttcgtttga
    45961 tagcaacaat tactactgtt ataagcttta tccttgtata cgcattaatt cacgaactta
    46021 tgccttttgt tgtgcgggca atggatttaa tgtaccacca gtaaatttag tgagtaggta
    46081 aaatgaacta ttcaatcgat tctataatta aaaatgaaag taaagtaacc aaatattgaa
    46141 gaggtgtaca aatgaaaata tatagtcaag gtgaccaagc cattgtagtc gcaattgaaa
    46201 aagaagtatc taaaagttta accgaagatt tattaacact tcgctcatat ttaattgaac
    46261 aaaattatcc atttattata gaaattgtgc catcagaatc agacatgatg attgtctatg
    46321 acgcaaggga tatgattaaa caccataata tacaatcacc ttttttatac atgaaagcac
    46381 taatagaatc gattcattta aacataaaac atgattttaa ccagcaagat ttgattgaaa
    46441 taccaattgt gtatggttcg aaatatggtc cggatttaga atcactttta aaacattaca
    46501 aaatcaagct agaaactttt attgaattac attctaaggc gcaatatttt gtttcgatga
    46561 tgggatattc acctgggttt ccttatttaa ctggattaaa taagaaattg tatattaatc
    46621 acacgagtaa acagaaaaaa ttcattccag ctggttctgt agtacttgaa gggaaaaaat
    46681 gcggtattgt aactacggat acaattaatg attggttagt tattggttat acaccattat
    46741 cactttttaa tccgaaagaa tcagatttcg cacgcttaaa gttaggcgat aatattaaat
    46801 ttagacctat caatgaaaat gaattagaag taggagcgtt taaagatgtc aatcataatt
    46861 gaaaaaagtg gcttattcag tagctttcag gactttggca gaaggggata tgaacatgat
    46921 ggtgtaattc catgtggtgc acttgatact ttagcacatg aaattgctaa tcgattagtt
    46981 gcaaatgaca agaatgaagc aactttggaa atgactaata aaatggcaac gattcgtttt
    47041 acagaaccta cgctgattgc attagcaggg ggtaatgtca aagcttacac tgagcatatg
    47101 actatatctc catataaatt gtatttgtta gataaaggcg atgttttaaa gtttagagaa
    47161 acaagttata catcgcgagt gtatttagct gtgggaggcg gatttgaatt agatgcatgg
    47221 ttaggatcta actcaaccga ctttaatgta aaaattggtg gttttaaagg tagaacatta
    47281 caagatggcg atgaaataaa gcttaagaga gattatacag ctcgtcatca taagttattt
    47341 gaaaaccttg ctcacacgaa acaaacagat tggggtattg atggatacgc cttgtcattt
    47401 aattatatgt ctgatgtatt tcatgtcgtt aaaaataaag gtacggaaga ttttaaagaa
    47461 gatgccattc aaagatttgt gaaacatgat tataaagtaa cgagcaaagc aaatcgcatg
    47521 gggatgatgc ttgaaggtga aaaaatcaaa gctttttatg aagatatgcc accgtatcag
    47581 actgtcaaaa aaggaacgat acaaattaag cgtgatggca cacctattat cctattaaat
    47641 gatcattata cgctaggtag ctacccgcaa atcggtacaa tcgcaagtta tcatttaacg
    47701 aaattagcac aaaaaccgca aggatcacgt ttgaaatttc aatttataga tattttaacg
    47761 gctgaaaaga accttgttaa gtatagtaac tggttaaacc aattattcca tggaatagaa
    47821 tatagaatgc aattagaaat gatgaaataa tatttggtac gtagctcata ctcgagtccg
    47881 atgcaaataa tttcctccta atgtataatg aaataatact gtgttttatc tgcgaaatgt
    47941 atcattttct aattcgtttc acagtaaaat gaaaagataa agtgtgtttt tacttgaatt
    48001 ttg
//
LOCUS       DALAAR010000011        47484 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000011 DALAAR010000000
VERSION     DALAAR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 47484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.011"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            53..1408
                     /gene="brnQ"
                     /locus_tag="O2458_000637"
     CDS             53..1408
                     /gene="brnQ"
                     /locus_tag="O2458_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ3886522.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            1661..2845
                     /gene="rocD"
                     /locus_tag="O2458_000638"
     CDS             1661..2845
                     /gene="rocD"
                     /locus_tag="O2458_000638"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ3886523.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            2884..3915
                     /locus_tag="O2458_000639"
     CDS             2884..3915
                     /locus_tag="O2458_000639"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCZ3886524.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            3927..5168
                     /gene="argJ"
                     /locus_tag="O2458_000640"
     CDS             3927..5168
                     /gene="argJ"
                     /locus_tag="O2458_000640"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCZ3886525.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5184..5948
                     /gene="argB"
                     /locus_tag="O2458_000641"
     CDS             5184..5948
                     /gene="argB"
                     /locus_tag="O2458_000641"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCZ3886526.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6218..6697
                     /locus_tag="O2458_000642"
     CDS             6218..6697
                     /locus_tag="O2458_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCZ3886527.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(7025..7669)
                     /locus_tag="O2458_000643"
     CDS             complement(7025..7669)
                     /locus_tag="O2458_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCZ3886528.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7682..14857)
                     /locus_tag="O2458_000644"
     CDS             complement(7682..14857)
                     /locus_tag="O2458_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCZ3886529.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15304..16554)
                     /locus_tag="O2458_000645"
     CDS             complement(15304..16554)
                     /locus_tag="O2458_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3886530.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(16940..17965)
                     /locus_tag="O2458_000646"
     CDS             complement(16940..17965)
                     /locus_tag="O2458_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCZ3886531.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18138..18497)
                     /locus_tag="O2458_000647"
     CDS             complement(18138..18497)
                     /locus_tag="O2458_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ3886532.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(18714..19745)
                     /locus_tag="O2458_000648"
     CDS             complement(18714..19745)
                     /locus_tag="O2458_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ3886533.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(19758..20519)
                     /locus_tag="O2458_000649"
     CDS             complement(19758..20519)
                     /locus_tag="O2458_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3886534.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(20516..21490)
                     /locus_tag="O2458_000650"
     CDS             complement(20516..21490)
                     /locus_tag="O2458_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3886535.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(21504..22244)
                     /locus_tag="O2458_000651"
     CDS             complement(21504..22244)
                     /locus_tag="O2458_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886536.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(22586..23098)
                     /locus_tag="O2458_000652"
     CDS             complement(22586..23098)
                     /locus_tag="O2458_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCZ3886537.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            23277..23480
                     /locus_tag="O2458_000653"
     CDS             23277..23480
                     /locus_tag="O2458_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886538.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(23542..24501)
                     /locus_tag="O2458_000654"
     CDS             complement(23542..24501)
                     /locus_tag="O2458_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ3886539.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(25147..26634)
                     /locus_tag="O2458_000655"
     CDS             complement(25147..26634)
                     /locus_tag="O2458_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ3886540.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            27061..27444
                     /locus_tag="O2458_000656"
     CDS             27061..27444
                     /locus_tag="O2458_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCZ3886541.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            27451..27777
                     /gene="isdI"
                     /locus_tag="O2458_000657"
     CDS             27451..27777
                     /gene="isdI"
                     /locus_tag="O2458_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCZ3886542.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(27842..28987)
                     /gene="cap8P"
                     /locus_tag="O2458_000658"
     CDS             complement(27842..28987)
                     /gene="cap8P"
                     /locus_tag="O2458_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCZ3886543.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(29064..30326)
                     /gene="cap8O"
                     /locus_tag="O2458_000659"
     CDS             complement(29064..30326)
                     /gene="cap8O"
                     /locus_tag="O2458_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCZ3886544.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(30380..31267)
                     /gene="capN"
                     /locus_tag="O2458_000660"
     CDS             complement(30380..31267)
                     /gene="capN"
                     /locus_tag="O2458_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCZ3886545.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(31267..31824)
                     /gene="cap8M"
                     /locus_tag="O2458_000661"
     CDS             complement(31267..31824)
                     /gene="cap8M"
                     /locus_tag="O2458_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCZ3886546.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(31835..33040)
                     /gene="cap8L"
                     /locus_tag="O2458_000662"
     CDS             complement(31835..33040)
                     /gene="cap8L"
                     /locus_tag="O2458_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCZ3886547.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(33041..34246)
                     /locus_tag="O2458_000663"
     CDS             complement(33041..34246)
                     /locus_tag="O2458_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCZ3886548.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            complement(34239..35405)
                     /locus_tag="O2458_000664"
     CDS             complement(34239..35405)
                     /locus_tag="O2458_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ3886549.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            complement(35419..36528)
                     /locus_tag="O2458_000665"
     CDS             complement(35419..36528)
                     /locus_tag="O2458_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ3886550.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            complement(36533..37159)
                     /locus_tag="O2458_000666"
     CDS             complement(36533..37159)
                     /locus_tag="O2458_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCZ3886551.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            complement(37162..38286)
                     /gene="cap8G"
                     /locus_tag="O2458_000667"
     CDS             complement(37162..38286)
                     /gene="cap8G"
                     /locus_tag="O2458_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCZ3886552.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            complement(38290..39399)
                     /gene="cap8F"
                     /locus_tag="O2458_000668"
     CDS             complement(38290..39399)
                     /gene="cap8F"
                     /locus_tag="O2458_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCZ3886553.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            complement(39412..40440)
                     /gene="cap8E"
                     /locus_tag="O2458_000669"
     CDS             complement(39412..40440)
                     /gene="cap8E"
                     /locus_tag="O2458_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCZ3886554.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            complement(40430..42254)
                     /gene="cap8D"
                     /locus_tag="O2458_000670"
                     /pseudo
     CDS             complement(40430..42254)
                     /gene="cap8D"
                     /locus_tag="O2458_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            complement(42274..43038)
                     /gene="cap8C"
                     /locus_tag="O2458_000671"
     CDS             complement(42274..43038)
                     /gene="cap8C"
                     /locus_tag="O2458_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCZ3886555.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            complement(43041..43727)
                     /gene="cap8B"
                     /locus_tag="O2458_000672"
     CDS             complement(43041..43727)
                     /gene="cap8B"
                     /locus_tag="O2458_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCZ3886556.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            complement(43743..44411)
                     /gene="capA"
                     /locus_tag="O2458_000673"
     CDS             complement(43743..44411)
                     /gene="capA"
                     /locus_tag="O2458_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCZ3886557.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            complement(44756..47365)
                     /gene="adhE"
                     /locus_tag="O2458_000674"
                     /gene_synonym="adhC"
     CDS             complement(44756..47365)
                     /gene="adhE"
                     /locus_tag="O2458_000674"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCZ3886558.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT        15109 a         8960 c         7010 g        16405 t
ORIGIN      
        1 atcttaatta agtagatttt tataagttct aaaaaggaga acaaatacat atatgaagaa
       61 gaaactaaca tttaaagaaa acatgtttat aggttctatg ttatttggtt tattctttgg
      121 tgccggcaat cttatcttcc caatacactt gggtcaagct gctggttcta acgtttttat
      181 cgctaactta ggatttttaa ttacagcaat tggcttacca tttctaggta tcattgctat
      241 tggcatttca aagacatctg gtttatttga aattgcatcg cgtgttaata aaacatatgc
      301 ttacattttc acgattgcct tatatctagt tatcggacca tttttcgcct tacctagact
      361 ggcaacgaca tcatttgaaa ttgcattttc gccattttta tcaccaaagc aaatcacttt
      421 atatttattt atttttagct tcgtcttctt tgtgattgca tggttttttg cgagaaagcc
      481 atcaagaatt ttagaatata tcggtaaatt tttaaatccg gtattcttag tattattagc
      541 aattatttta ttatttgctt ttatccatcc attaggtggc atatctgatg cacctattag
      601 taaacaatat caatcacatg ccttatttaa cggcttttta gatggataca ataccttaga
      661 tgcgctagcg tcattggcat ttggtattat cattgttgca acgattaaaa agttaggtat
      721 cgaaaatcca actgatatcg ctaaagaaac aattaagtct ggtactatca gtatcattat
      781 gatggggatc atttataccc tactagcaat catgggtaca ttaagtattg gtcatttcaa
      841 acttagtgaa aatggtggta ttgccttagc gcaaattact caatactact taggtaacta
      901 cggtatcgtc ctgttgtcac ttatcgttat ggttgcttgt ttaaaaacag ccatcggttt
      961 gattacggca ttttcagaaa cattcgaaca ccttttccct aaaatgaatt acctagcgat
     1021 tgcaacagtt gtaagcttta tttcgttctt attcgcgaat gttggtttaa ctaagattat
     1081 tatgtactca gtcccagtgt taatgttctt atatccatta gcaattgcct tgattgtact
     1141 aacattattt agtagcaaat tccatcattc aaaacttatt tatcaatgta ccattttctt
     1201 tacaatgatt gctgcattag tagatggatt aaaagctagt ccagagttca tttcaagcac
     1261 atcattctca caaactttga ttaatttcag ccaaaaatat ttaccattat cagacattgg
     1321 tatgggctgg gttgttctca gtttgattgg tttcattatc ggcttcatta tttataaaat
     1381 taagcatcgt aaaattccac aagcataata ctatgccaca gtcatatgtt aaacatatgc
     1441 ttgtggcatt ttttattcat actacattaa actgcaatcg tatacataca tatcaatgat
     1501 tatccacaaa aaatattagt actttcattt tacaaatcac attaatacaa acacaacctt
     1561 atctttatat tattaaattt acatttgaca cttatattga acaactgtaa tatattaata
     1621 ttaattcttt aaaatgtata aatataaagg agggagaccg atgaattcaa tcattgaatt
     1681 aactgattat tatagctcta ataattatgc accacttaag cttgtcattt ctaaaggtaa
     1741 aggtgtcaaa gtttgggata ctgatggcaa acaatatata gattgcattt cgggtttttc
     1801 agttgcaaac caaggccatt gtcatccaac aattgttaaa gcgatgacag aacaagcttc
     1861 aaagttgtct atcatttcac gtgtccttta tagtgacaat ctcgggaaat gggaagaaaa
     1921 aatttgtcat cttgctaaga aagacaaagt actccccctt aactctggta ctgaagctgt
     1981 tgaagcagcc attaaaattg ctagaaaatg gggctctgaa gttaaaggca ttactgacgg
     2041 acaagttgaa atcatcgcta tgaataacaa ttttcacggt cgtacacttg gctcattatc
     2101 actatctaac cacgacgcat ataaagcagg atttcacccc ctacttcaag gcactacaac
     2161 agtagatttt ggagacattg aacaattaac acaagctatt tcaccgaata cagcagcaat
     2221 tattttggaa ccaattcaag gtgaaggtgg cgttaatata ccaccgaaag gatatattca
     2281 agctgtgcgt caactatgtg ataaacatca aatattattg attgcagatg aaattcaagt
     2341 tggtcttggt agaactggga aatggtttgc tatggaatgg gagcaagtcg ttccagacat
     2401 ttatatttta ggtaaggcat tgggtggcgg cttataccct gtatctgctg tacttgcaaa
     2461 taatgatgtc atgcgtgttc taacaccagg tacacatggt tcaacatttg gtggtaaccc
     2521 tttagccatt gcaatatcga cggcagcgct tgatgtactt aaagatgaac aactggttga
     2581 acgatcagaa cgcttaggtt catttttatt aaaagcgttg ctacaactta aacatcctag
     2641 tattaaagaa attagaggtc gtggtttatt tataggcata gagcttaaca cagatgctgc
     2701 accttttgtg gatcaactga ttcaacgtgg aatcttatgc aaagacacgc atcgtactat
     2761 cattcgattg tctccacctc tagtcattga taaagaggaa atccatcaaa ttgttgcagc
     2821 ttttcaagac gtttttaaaa attaacaatt aatcatttat atatgacata ggagggattc
     2881 atgatgatta aagtaggtat cgttggcggt agcggttatg gcgcaattga attaattcga
     2941 ttgttacaaa cacatcctca tgtaacgatt gcacacatct actcacattc aaaagtagat
     3001 gaaccgttga aattaacatt tccacattta caacatatta tgcaacattt cgaagcactt
     3061 acagtggaca ataatgactg tgatgtaatt ttctttgcga caccagcacc cgtaagtaaa
     3121 acatgtatcc ctcccttagt agaaaaaggt attcatgtta tcgatttatc tggcgcattt
     3181 agaattaaga atcgtgaaat atatgaagca tattacaaag aaactgctgc agcacaagat
     3241 gatttgaatc atgctattta cagcatttca gaatggcaat cgtttgataa caatggaacg
     3301 aagctcattt ctaatcctgg ctgtttccct acagcaacat tattagcatt acatccactt
     3361 attagcgaaa aaatagtaga tttgtcatct attattattg atgctaagac cggcgtgtca
     3421 ggtgctggtc gttcattatc acaacgtgtt catttttcag aaatgaatga aaatctaagc
     3481 gcttatgcaa tcggaaacca taaacacaaa ccggaaatcg agcaatattt atctatcatt
     3541 gcgggtcaag atgtatcagt catatttaca ccacatctcg taccaatgac acgaggtatt
     3601 ttatcaacaa tatatgtcaa attatcatct gaatatacga ctgaatcatt acataaatta
     3661 atgacctctt attatgctaa tcagccattt gtcagaatta gagatattgg gacttttcca
     3721 accacaaaag aagtactcgg tagtaactac tgcgatatcg gcatctatgt agatgaaaca
     3781 acgcaaacag caattttagt atcagtgatt gataaccttg tcaaaggcgc aagtgggcaa
     3841 gccattcaaa atttaaatat attatatgat tttgaagtga cgactggcct aaatcaatca
     3901 ccagtttatc cataaggggt gttagaatga aacatcaaga aacgacatca caacaatata
     3961 acttttcaat tattaaacat ggcgatatca gtacacctca aggcttcacg gctggtggta
     4021 tgcacatcgg tttacgcgct aacaaaaaag actttggatg gatttactca tcgtctttgg
     4081 caagtgcagc tgccgtatat actttaaatc agtttaaagc tgcaccactt attgtcactg
     4141 aagacacttt acaaaagtct aaaggaaaat tacaagcact tgttgttaat tcagctaatg
     4201 caaattcttg taccggtcaa caaggcatag atgatgcacg acaaacacaa acatgggttg
     4261 ctcaacaact tcaaatacca tctgagcatg ttgctgttgc ttcaactggg gtcattggtg
     4321 aatatttgcc tatggataaa attaagactg ggaccgaaca tattaaggat gctaattttg
     4381 caacgccagg tgcgtttaac gaggcaattt taacaactga tacctgtaca aaacatatcg
     4441 ctgtatcact aaaaatcgat ggtaaaaccg ttacaattgg tggtagcacc aaaggttcag
     4501 gtatgattca cccaaatatg gctaccatgc ttgcttttat aacaaccgat gcatcgattg
     4561 aatcgaatac acttcatcaa ttattaaaat cttcgactga ccatacattt aatatgatta
     4621 ctgttgatgg cgatacaagt acaaatgaca tggtattagt catggcaaat caccaagttg
     4681 aacaccaaat acttagtcaa gaccatccac aatgggaaac atttgttgat gcattcaatt
     4741 ttgtctgtac atttttagct aaagctatag ccagagatgg cgaaggcgca acaaagttaa
     4801 tcagcgtcaa tgtgtcaggc gctaaatcaa tatcagatgc aagaaaaatc ggtaaaacta
     4861 tcgtaagttc aaatctagta aaatcagcta tttttggcga agatgccaat tttggtcgaa
     4921 tcattacagc tattggctac agcggatgtg aaattgatcc taactgcaca tatgttcaac
     4981 tgaaccaaat acctgtcgtt gataaaggta tggctgtact atttgatgag caagctatgt
     5041 cgaatacatt aactcatgaa aatgtcacaa ttgacgttca gcttggttta ggtaacgctg
     5101 cagcgactgc atacggttgt gatttatcct atgattatgt gcgtatcaac gcatcatatc
     5161 gaacataagg tggtgttggt tagatgaaat ttattgtcat taaaattggt ggcagtacac
     5221 ttagtgacat gcatccatca attattaaca acattaagca tttacgatca aacaacatct
     5281 accccattat cgttcatggc ggtggcccat ttattaatga agcattatca aaccagcaaa
     5341 tcgagccaca ctttgttaat ggcctaagag tgactgataa agcaaccatg accattacta
     5401 aacacacgct cattgcagac gttaacactg cattagtagc tcaatttaac cagcaccaat
     5461 gttctgcaat aggcttatgt ggtttggatg cacagctgtt tgaaattaca tcttttgatc
     5521 aacaatatgg atatgtcggt gttccgaccg ctttaaataa ggatgcttta cagtatttat
     5581 gtactaaatt tgtacctatc atcaattcga ttggtttcaa taaccatgat ggagaatttt
     5641 acaatattaa tgctgacacg cttgcctatt ttattgcatc atcattaaaa gcgcctattt
     5701 atgtattaag taatattgca ggtgtactca tcaatgatgt tgttatacct caattgccat
     5761 tagtcgatat tcatcaatat attgaacatg gtgatattta tggaggtatg attcccaaag
     5821 tgctagatgc caaaaatgcg attgaaaatg gctgtcctaa agttatcatt gcatcaggaa
     5881 acaagccaaa tatcattgaa tctatttaca ataatgattt tgttggcaca acaatcctta
     5941 attcataact atgaaattaa ggcctaacaa gttttgacac gcgagatgat tccagttcga
     6001 ttatccattg cgctaaaaca tttatttacc gttcatctcg ttaacaattt tgaatacagt
     6061 acgatacaat atgagatgta aaaaactaat aaccttttac aaatttgttt atcaaaatat
     6121 tttaagtttt gcaaagcttt ttattgtgat tattttcaca aaatactata atgaggatag
     6181 taaatagaga ggagtcctta agttgacgaa acgacaaatg ggtatattca tttatgctgg
     6241 aattatcggt ggcttgttat ctggaattgt aaaattaggt tgggaggtca tgtttccacc
     6301 tcgcacacca gaacgtaatg caacgaaccc acctcaagag ttattgcaac aattaggatt
     6361 tagtagtgag tttacgcatc aaacatatac attttcaaat atggaattgc cttgggtaag
     6421 ctttattgtc cactttagtt tttctatcgt cattgcaatt atttactgca tattagttaa
     6481 aaaatacgct tacttagcaa tgggacaagg tgctgttttt ggtattgcta tttgggtatt
     6541 attccacctt atcattatgc caatcatgca tactgtacct gctgtgtggg atcaaccatt
     6601 ccaagagcat ctgtcagaat tctttggcca tatcgtctgg atgatgacaa ttgaattagt
     6661 gcgacaacat tttgtctatc gctataaatt aaattaatac actgactaac attaacgtga
     6721 gttttaaatc atcgtttgag tatgatgatt gatgctcacg ttattttatt aactgacatg
     6781 atatgattcc agccaactta cgtgagcatt aaagtctcaa atgcgtcgta acaaactatt
     6841 attttcggta atttcaatat tgctcagtat atttttacct tatcacttac tttaatctcg
     6901 tcatgatttt gaatgatgcc atcgtgtatt cacctttcat ttttccaata aaaaaacatc
     6961 taacagtaaa catttaggca gtatagttta aaatcactgc gcaatgatac tgtcagacgt
     7021 catattaact actcaataac tgaaatacag acactttttt ataaccccag ggtgcctgtc
     7081 ctaagaaaca tacctgtacc ataaactgat caaaaataaa ttgtttgaac ttcacttcac
     7141 gtgattgata aaagtgtgat tgtgtcatat cataaatgtc caatcctttg attaaacctt
     7201 caccaatcaa ttttgtaaaa ctttcttttt gtgtccatat ttgataaaaa tcatttaaac
     7261 tacatatttg atgtgcttcg tttgtagaga aacacgtcac taacgtacgc cagtctaaac
     7321 gttgtgatat cttttcgata tcaataccaa ctggttcttt atcgacaaca cacacgatat
     7381 aaggataact atatgataag ctcacataga tgggctgtcc atcacgattg tgttgaacaa
     7441 tatctgcctt acctcgtggc gaaatgtgat aatgccattc atgtggtaat aaacctgtgt
     7501 catgttgaat tccatattgc actaaaatat ctcccaatct gtgcatgagt ttatcttgat
     7561 tgtatctata gttgactgta cgcggttttt tatatgacca acgactttgt gatattaatt
     7621 cttcaatact tttcaagtta ctctgtaatt gcattacaaa tactgtcata actttcccta
     7681 cttacttatt gaatattgtt ttgatatatt gtgcccaatg atacagccaa ttgttagtta
     7741 tcgttggcca tttttcactg atgtgattca ttatttttaa tgttaatgtt gtatctatca
     7801 ttgctagttg ttgttcacgg tcaacactag ttaatccaat cgtttcgtac atgtcttgtt
     7861 tctgtaaaat ttcattaaat gattcatcgc tgacgagttc aatttctttg cgcttaacgc
     7921 attctaacaa agatttcacc ggcattttat taggtgatag cacatggtaa atgatttgtg
     7981 gtgtgttgac ctgtgctaat gcgacaattt gtcttgcagt cgtatccaca aaagaaaaat
     8041 ctacaggcat ttcagccatg ctaaccccga tacaatccag ttgtaacaaa tcattcatta
     8101 ccattgaaaa acggttagtc tttatatttc tcatatgcca tcttccattg taaggattcg
     8161 tcaaattacc aacacgtaca atccgaccat ctaagccatt atttacagct tctaatactt
     8221 ttaattcact ataaaatttg ctccgtgtat atggtgatgt tagtagttgc cctttataga
     8281 catccgcttc tgaaaatgtc acatcttctg tgtctatatc aaaataagtt cccacactta
     8341 tcgtagacac atatattaac cttgcatgat gttgttgtgc caaacgtatg acatcaacag
     8401 taccttgaac atttactttt tcaaattcat catcatcacc aaagtgatct gtacgagcac
     8461 ctgcatgaat aatcgtatcc atgttttctg gtaaaacaac atcatccata cactcgaaat
     8521 caccaacaat gacttcaatg tttgataaca ttatttcaac cgtctcttct gaaaaataat
     8581 catttaaatt cgtcatcaac ttataccatg ctatttcctc attatcagca cgtatgaaac
     8641 aataaatgcg atgactgtat ccttgtagta cttcaatcag ataagcacct aaaaaacctg
     8701 tcgcgccagt caatagtgta tttcctagag gtcgatgact tagactatcc tctaaaatac
     8761 ccaagttata acgagacata acaatctttt gtaattccga aagattatcc ggtaatgcaa
     8821 ctaatgattg ttgattttgg tacatataat taacaatctg tcgcacggtt ttatattggt
     8881 ataatgtctg cattgaaata tgatggccaa atcgttttaa atgcgagaca actaacatcg
     8941 cctctaatga gttaccacca agttcaaaga aatcatcgtc aacaccgaca tcattttgtt
     9001 tcaatacctc tccaaataca tcaacaaatg tctgctcaat ttcattagag ggttcgctat
     9061 acactttatt agactgttgt ataggtgatg gatttggcaa acgcgtagta tccaccttgt
     9121 cattcgtggt taatggcata caatcgatat gcgttatagt cttaggaatc atatacttag
     9181 gcagctgatc atttaaatat tgcttcaaat cctgttccac ttgttgctct ccgacataat
     9241 aagcattcaa tatatcatgc gtatcaaagt gacttactgt tacaacacaa tcagatatac
     9301 cacgaatagc taatattgca ttttcaattt catcaagttc aatacggtac ccgttaactt
     9361 tcacttgttt atctattctt cctaaaaatt caatttgacc atcagatgta taacgtgcta
     9421 aatcaccact atgatacaac tttcctttac caaatggatt attttgccat ttatcagcca
     9481 ttaattctgg acgattaata tatcctatcg ctaaactatc acctgcaata cacaactcgc
     9541 ctggcatacc aataccgcat aacaaaccat ctgacataat atacacttgg atgttagata
     9601 agggtttgcc aattggaatc gtctcaggta tcaaatcacc acaatgatgt gaccaatacg
     9661 atgtgatgac tgttgactca gatggtccat aggcattgaa atacgtgcca caatgcttct
     9721 caatatattt aacaaaggat gccgtactag ttgccccgcc tgtaatcaac ttttcaatat
     9781 aaaagtcttc cataacacta cacatctgta acggaatcga cgcaaccgtc acacgatgct
     9841 tattaatgag ttgttgtaac tgttctggat taacacgttc ctctctatct ggaatcacaa
     9901 gcgtatgacc atttaacaaa caacaataaa tctccataac tgatgcatca aaaacaatat
     9961 ttgcatgttg caaaaatact tcattgtcgc ctaattgcaa ttcagttgac catgcatgca
    10021 ctaaattcaa caaatttcgt tgtcgtatgg caaccccttt aggcatcccg gtcgtaccag
    10081 atgtgtaaat agcatacatc tcattatcta acatcgctgt gttttcaagt tgattgccat
    10141 gtaaatcatc atattgttca ttttcctttg attcaacaaa gcctttagca ttttccaatt
    10201 gaatgactgg taatgtcgtt tcaatttcaa cgccgtaaga catgactgca gttactttag
    10261 catcctccaa aattgcacct tgtcgtttat tcggaaaatc aatatcgata ggtatataag
    10321 atgcacctac tttaactgtc gccaacatcg ccgcaatcat ttcaaaacta cgttctgtaa
    10381 acaaggcaac ccgttgacca ttgcccacac catttgatag gagcatgtgc gcaatggcat
    10441 ccacatagtt gcgtaatgtt tcatacgtca ttgtcaaatc attcatgact agcgcaacat
    10501 gattaccttg tcgtgagaca acttcattaa agtaacttat gatagattta tttcccggga
    10561 cattaagcat tcgatcgtta acatgcgtat tgacccaatt tagaagttcc tccgtgccgt
    10621 ttggtatatc acaaatttgt agtgtatctt gatgcttcaa aatataatca atcataatca
    10681 tacattgatt acccatgtga cgaactgttt ctgagtgata taaatcggta ttatactcga
    10741 tattgattgt atagtcatcg cgatcttctt caatgatgaa agataaatca aatttcgccg
    10801 tcactgattt gggttgaatg tgtgttaatt tactatgccc aaaatgagca tgattcgttt
    10861 cattgttttg tagtactaac atgacatcaa ataatggatt ccgtgaggca tcatgtgatt
    10921 gatctaagtc atttactaaa cattcgaatg ggtattcttg atgctcgtat gcctccaaac
    10981 tcatttcctt aacctcttgt aaaaactgtg tccacatttt atcaggtgac ggttgccctc
    11041 tatataccaa cgtattagca aacatgccta gcatttgctc cgtgccttta tgcatacgcg
    11101 cactcatcac actaccgaca acaacatcat cttttcgagc atatctactt aacaacgtca
    11161 tgaccacact cataaagaac ataaaatcag taatttgatg cttttctaca tacttttgaa
    11221 gtagctgtct catttgttga ttcattgtaa atgacatcat tgctccattt gtcgttttaa
    11281 tatttggtct aacatagtct gtcggtaagc ttaaaatagg tacttcatct ttgaattgag
    11341 ataaccaata ttgtctatgt ttcgtcatat cacgatgcga catccactca ctatagtctt
    11401 tatattgcaa tttaagtggt aacaataatt tatgttgata aagtgcgtta agatcattca
    11461 ttaattgtat attactcata ccgtcattaa tgatatgatg cgtatctata aagaggtatg
    11521 catgtaaggg acttctaatg tatctcactc taatttgact tggcttttcc aaattaaaag
    11581 gtgctacaaa ttggcgcatg atttcttgtt catccgtaaa atgcgtgtta acttcttcaa
    11641 agtcaactgc aacatctgcc acaatacgtt gtcgaacctc atcatctaca acaatatatt
    11701 gtgttcgtaa aatctcatgt cgcgctatca aacgctgcac tgcttgtcgc aattgagcta
    11761 cattaagttc tgatgataac cgccataaaa aaggtacgtt atacaccgta tctttatggt
    11821 ttgatttcca taataaatac atacgctttt gtgcagagct cagcacataa tcatctttaa
    11881 ctatagtttc tggaatcact tcatagtttt gttcttgaac cttagcaatc gcttgtgcta
    11941 gttcaaatac agttggcttt tgtaataaat caccaatttg taatcgtttc ccagtagatg
    12001 cctctatccg attcaccact aacgttgctt ttaatgaatg gccacctaat tcaaagaaat
    12061 tatcatgaat acctacttga ttcacatgta aaatatctgc aaaaatttgg catagcaagt
    12121 gttcggtatc tgtactcggt gctacatagg catccgtatc gacatagtcc atgataggca
    12181 atgccttctt atctaatttc ccattaatag taataggaat ttgctcaata tgcatgaaat
    12241 taactggtat catgtactcc ggtaaggtca tacgtaattg tgatttaatc ttattatgtg
    12301 ataatgtatg catcgcttca taataagcaa cgatatactg atcttgatca tgattttgaa
    12361 caataacaac tgctttatta ataccttgta tacgctcgag cgcatgctca acctctgaca
    12421 actcaatcct aaaccctcga atcttaactt gtttgtcctt tcgatataaa taatctatgt
    12481 tgccatcggg taacaaacga acgatatcac cacttctata catcagctga tttatatttg
    12541 aatctttgat aaatttatct gctgtcaatt ctggctgatt taaataacct gcagctaacc
    12601 caaagccact tgtacataat tctccaggaa taccaacgcc acaccgacgc tcgccttgca
    12661 tgatataaac atgagtaccc agaatcggtt taccaatagg aatacgattt ggaactttgt
    12721 taggtatatt atacgtcgtt gtaaatgttg tattttcagt tggtccataa ccattaataa
    12781 tttgaggatg cttcggtttt tgattaagca aatccaccca cttagcattc aatacttctc
    12841 caccaattaa taaatacttt aacggtacca atacttctat tcgttcacta gcaatctgat
    12901 taaataatga ggaggttaac cacatagtat taacgtcatt ttcattgatt aattgttcta
    12961 ccgctattgg atttaataat tgttcttttt tagcaacaat cagctttcca ccattgagca
    13021 atgcaccata tatttcaaat gttgcagcat caaaggctat agttcctgat aacaaaatcg
    13081 tcgtctcttc atttaatggt acataatgat tttgatggac caatcgaaca atacctcggt
    13141 gcggaattag tgtcccttta gggttaccag ttgtccccga cgtgtaaata acataagcat
    13201 gatcttctaa cgtgttacat ttagaaagat tatcaatatt tttccacgct atcttattca
    13261 aatcaatgtg attaatattt tgtttaccat tttcatataa agcttggtac gttattacaa
    13321 ctttaggcgt tacatctttt aaaatgtact cctgacgatc acttggatag ttcggatcaa
    13381 ttggcacgta agccccacca gctttcaaca cacctatcat cgctattatc atctcaatac
    13441 ttttttcagc tatgacagcg acacgatcat taggttcaac accatactgg tttctcaaac
    13501 ggtgtgctaa atcattcgcg cgtgcattca atgtttgata tgttataaac actccgtcaa
    13561 attgcacagc gacatgattc ggcgttgctt caacttgttg ctcaaataag gtaacaactg
    13621 tttgcgcatc atctatctca ggcaaactta aattgatatc gtcatataat tgaatatcac
    13681 gttctgtcat caaattaagt tcatctacag ttgttcgttt atttccattt tcttcagtaa
    13741 tttgcaaata aatatttcga actaagtcac tcagcgtctc gattgagagc aaatcatatg
    13801 ctgcactgtt atatataatt ttgaacccgt gttgatgtgg aaaaatttca atatccgcta
    13861 aagatgtatg tgcatcatgt atttgatgta catcctcaat aacatcatta caacaagaca
    13921 tcatatgatg ataacaatga aaaatagtct ctagtgaaag cgaagacttc gcgcactgta
    13981 attgcgacat attttgcaac acacatttat taaaatctgt tgtaaaacgt tgacatacat
    14041 cttttgcatc gattgttaac gttaacggca caatatttcc gtgtaaatca tttggtaaat
    14101 gtgatggtac atgtatacct aatgtgacat catgttgttg actcattata tgattagcta
    14161 aatacacact aacagccaac gatgccatat ctattgatgt catatcatca atcaaatacg
    14221 tttgatataa agcttgttca aatggatgct taattggaaa ataactatca atatggatgt
    14281 cagagttatt ctctaaccga aaatagtttg agtctaatgc tatatgcgat gcatcttgat
    14341 tgtctttatc atcatttcta tttatatgtg catgctgtcg agtattgtta ataacagtat
    14401 tgccacgata tgcattgcat aaatcatcaa gaaaaatatc aatttgacta tcatcgaaaa
    14461 tggacacatg aaaatctaat agtatatatg cagcatcagc gaactgaaac aatttaactt
    14521 tgaataaagg tgaatcatta aaatggtaag tacttaattc ttgcttaaaa aaagcttcta
    14581 aatcatagtt tgcggaagaa gatggaactt gttttatctc aataaaaggc agaaattcat
    14641 gaagtatcat tgttaaattg tcatcggtag taacatcaaa aaaatgtctt atagatgcat
    14701 gttgttgcac aattgtcgat aatgcataca tcattttagt agcttcaaca tttttagcga
    14761 gtttaaccca atacgcatta cggtgtgtcg ttgattctgt attatttttg tatatacgaa
    14821 aatattcctg ttgaaatctc aaattaccca taatcataaa aagtccttct ttcatatcat
    14881 aatactcatt acttactgaa attgcatgat gatatgataa ccgacgaaat gttaattaac
    14941 tcgttatgta atgattaata taaaacacca ttcgcaacat atgagcgata tattctaccc
    15001 taaaatacat cttgtatcat cgttacaatt ggtatatttt tcaatgtaaa ttacatacat
    15061 cttcgataaa tagcacacta caactcgtta atcactttct gttgttcaca tctcattgca
    15121 aactcaatat tgttgttaca aaatatccat gaagcaagtt tatattaaac aaacaactcg
    15181 cataaaacaa ttgttatcct taaattttaa caaattctta ataaatttat ctctatttta
    15241 attacgacca aattaatagg ttttccatat aaaaagatgc ataaaataaa tatttaaata
    15301 aattcaattt gtattcactt gtttttgtcc cccaaataca ccagcaacaa gcatgctagc
    15361 accaattgtt aaaacgataa acatatacag tcccatttgt aatgacgtta agaaaacacc
    15421 caacacaatc cctaacctag ctagtgtttc tgaaaaatga atacctaatg cattaactgc
    15481 actatatgtt cctcttttag ctttaggaat aattttaaag cgttgttctg aaactatagg
    15541 cgaataaata atttcaccta cagtcgcaat tatcataaaa acaactaata agccaaactg
    15601 attaagatag gttagaccac tatagccaac aatataaatc agcaaaccag tgattaaagc
    15661 ttttttaaaa tctattttca atacaacttt cgagattgaa tacgtgagta aaatgacgac
    15721 gaccgtatta atcattagca agattgctaa catcttagca cctgtaatat catatgaacc
    15781 tatacttatt gtttcaaact gatcctttag tctaatagca atatatgagg agattgaaaa
    15841 ttcacccatc atgatgatac tgaaccccga aatcaataac atataattac ggtctttcaa
    15901 aactaattta taactgcgaa atatattcat tatttgtaat ttttgataac gacttgcatg
    15961 cctcttgtca tcactttgct ttacttgatt tcggtcttga ggtaaccaaa tatataaaat
    16021 aaagagtaca attaaaaata tacaagctgc tattaagaaa agtagtaaca tactgtagcc
    16081 atacatcaag ccacctaaca atgccccaat agctaccgat aagtttgtca tccaatagct
    16141 aatcttgtaa atataatgtt ccacgtcttc ggtaattgca tccataatta atgtgtccat
    16201 aactggaaat tgtaatcccc aaacgattgt aaatatggca tatgcaacac aaaaaccaat
    16261 aatttgccac aattgatgtg acccaaatac gcccatgaac acaagcatta tcaccatcgt
    16321 cgcttgataa ataagtacta gcaacttttt cggaaatatc tcaataaggt aaccagatat
    16381 aatggacaat ggaaatttta gaaccactaa accaacaaga tatataccga caattgattg
    16441 acttaacata tctgttaaat atagtgctat aaacggtata aatgctgtcg taataattag
    16501 ctgtaaaaaa ttgctaatca atcgtacttt caaagtcgta cttaagcgtt tcattaataa
    16561 ctacacccct ttccacatat atacacacta ccatttatat aaaatctatt attagattat
    16621 acgttgaaac aaagaatttt tagttataca aagttattta acaaacaaat gcatcgactt
    16681 tacaatgcat cttgcatata atgcctcaaa aaaattagca ctatatgact atatattatt
    16741 ccaaatgaat gaaatttatc aataattgcg taagtgaaca catatgtgcg taaatgttaa
    16801 atcattgtcc taacatagct gtgactatac ttagatgtaa tcataaaaaa actactgcca
    16861 tctttacaac agcagtagtg cttatatgat tcatttatat aaaattgaaa gcgttaatcg
    16921 ctagcccagc atccttattc tatttagctg tataactttt actagcaata cgaccacttg
    16981 caacaataat atctttatct tggaaaggtt catgattgaa gaaacgctct aaaatatctt
    17041 taactccatc ttcaatacgt ttttgtgctt ctaaagtcat acctgaatag tgaaccgtca
    17101 tagcatttct aggcattgtt ctccatggat gatcagcagg tgcaggttgt ggataccaaa
    17161 catcaccagc atatccttgt aaatgctcgg atgctaacgc ttcaactaac gcatcgcgat
    17221 ttacaatttt accacgtgca gtattcacta aataactgtg ttttttcata cgacttaaaa
    17281 catctttatc aaataagtta tcagtttctg gtgttaatgg tgcatgaatt gtaatcgcat
    17341 cacttgttga aacaagttca tcaaagctta caaatttaga caatttatgg tcttgttgat
    17401 tgattggatc atagtgttgt aatgttacat taaatggcgc taatctttca gcaacaagtt
    17461 gtccaattcg accaaatcca aaaataccaa ttgttttgtg ttgtaattca tgcgcatgat
    17521 tacctacttg agacaagttc cattcacctt ctactgattg acgatgacct tcttcatagt
    17581 ttctaagaag tattaataaa tccataaccg catgttctgc cacactaact gtattacttc
    17641 ctgtaacttc aacgacacca atattgtgtt cacttgctgc cgctaaatct acatggtcag
    17701 atcctacacc tgctgtaatt gctaatttca agttcggtgc tttttcaata cgttcacgag
    17761 tcatatatgc aggataaaat ggcgcactaa taatcacatc catatctggt aaatgtttat
    17821 ctaagtcttc accattatct gctaatataa tgaactcatg tcctctttcc tctaaaaatg
    17881 tttttaatcc taatgctttt ttagtattaa gtaattgatt ttcttgacct tctactgctt
    17941 ctggaaataa tgctacgatt ttcatatcca tctctcctaa atgataaatt ttgacttcct
    18001 taaagtacgt gttgcatgtt gcttgtttta aaaatatgac aaaaaaacgg caccgtttga
    18061 cataccaccc ccttgaatta ttgttaaatt caaagcgaag ttagcaaaca gcaccgtgta
    18121 ccttacacca gcaacactta ttctattgac ttatcgaaaa caaatattga aatgacttca
    18181 tcatcattca aatcaatatc tgtaaataat tttacaaact tagcgcctac aagactttcc
    18241 atctcattta catctatttc tttatacatc tgtttaatct tctctgtgcg tgtataatgg
    18301 agcatcgatt cattgcgttt gtcgtttagg taaaaactct caactttact caaaacacct
    18361 gtcatatgtg caatcgccca attatcttta aacgacactc gaatactatt gggtccttta
    18421 cccacgtatt ctttacgata agatcttact agattcgaga attgttgttc tttagtatgc
    18481 gtatctatag tattcacctt ctttaaaata agaagttcat taattgttca cattttcatt
    18541 atacacctta ttaacacttt ttcaatgata acgattatta ttattacgaa atgtatattt
    18601 tcctagttaa aaggatataa caaattaaca gaaaatgttt ataaactaag atttatatta
    18661 ttttaaaaaa tgtttcaaca aacgcatcta aacaaaaaat cagcttatca cacttacccc
    18721 ttcaactctg cttcaagttt acctaaatgt cttaatgtcg gtgtcaatgc tgcgaagaag
    18781 aatccttctt ttaacttgcg aacttgtggc gaatatggtg tgtacgctct agaaccacca
    18841 ttgacaacag aagcttgatt tacatctaac aataaatagc cgatgtcctt cttcaatgat
    18901 attaattcat ttaaatgtga agttaagtta ccgtcatcta atattgcata atattcctct
    18961 ctaagttgac gataacgttt tttaaaagct tcaacatcat actctaaata ttgatttatt
    19021 ccgttttgca catttgaaaa tgcatcaatt aactctaaag aacttttaat tgagcctaat
    19081 cctattggaa tttggtaagc aataaattgc gggcgaatag ttgccgcaaa ctgcttcgca
    19141 tcatgcgtga taatttgtga ttgtggcact acgacttgat tcaatgtgat ttgatacgtt
    19201 gctgacccgt tgactcctaa aaaattggtt ttttcaacaa gagtgatacc atcttgattg
    19261 gcacgtagaa tgaacatgac aaattcatct gatgattcat gtttcgaaat cgcaccaaaa
    19321 taatggtctt cttgaatatt acttacagct ggcatacgtc cactgacaac caattgtcca
    19381 tcaacataag tgtgttcaag gttcaacttt tctaaatcat taaatgactt catcggatta
    19441 gacaatccgg tagcacctaa tatttctcca gataacaatt gctgttgtaa gtcattattt
    19501 aaatgtggct gcgtggcatt ttctaaatac gttgaaaaag ctaattggca ccataaacaa
    19561 aatcctgttg tcaaacaaga ctgcgataca gcttcgatta cttcagcatt ttttctcaat
    19621 gccgcctcac cgaaataacc atctacaaat aattgctgaa taaatgttct cggataatac
    19681 gtcccttcat caacttctac taaatgagga tctaattgtt ctttgataag cgtttctaaa
    19741 gtcatcatca tctctcctta ttcaccaaat cttctaagta taaactgctc aatataacta
    19801 atgaatcgat caataataaa accaaataat ccgataaaga atattgctgc taaaacatct
    19861 tctaagttca acatatttcg tgcatcaacg attaaaaaac ctaatcccga ttgtgcacca
    19921 atcatttcac cagaaactaa aaatatccaa cttgttccta ccgccatatg tatcccagcc
    19981 atgatttgtt taaatgcccc gggaaataat atattgcgat acaatgacca cccagttaaa
    20041 tttaaatttg ctgctatttt taaatattga ggttcaatgt ctctaacgcc tttaatagta
    20101 ttgaacacaa ttgggaaaaa agcagcgata aaaataatcg caatcgctgg caaactacca
    20161 ataccaaacc atagaacaac aaatggtgcc catgctatcg gagatatcgg cctaatcaat
    20221 tgaaatagcg gttcgatagc gttgtatagc caacgattcc ttccaagcaa gaagcccaat
    20281 ggaatagcaa ccaacaatgc gacaacaaag cccgctacaa atctccataa actaattgct
    20341 aaatgttgga aaatttctcc agtaacaatg aaagaccata tactttttcc tacaagagca
    20401 ggacccggta acaatacagg ttggtaatgc ccaataataa tgaccatttc ccaaatgcct
    20461 aagaaaataa taaatgtgat aataggtaat ataaatttgt tatttgtggg acgtgtcatg
    20521 aacgcgatgc ctccttatac aatgacggtt caacaaagtc atcatatgca ggtggattaa
    20581 acaaatgatg ttgttttacc aatgtcgtaa tttcttgata gccggatggc ttaattgtta
    20641 aatcaccata ggatgtccat gccgctgact gtgttaaaac gtcacgactt tgtttaaaat
    20701 gatgcgtcat aatgtctaca ctttgcttgc gatcattcat tttaaagcca gactttttat
    20761 aatcttgtac aaatgcttgc gctacatcct tgtgttgatc aagcaattcc cctctcagta
    20821 ctagcacaca gcaatacgca tcaggtataa cgtcatcacc atgtttcaaa gtcttacctt
    20881 tgcctaactt ttcacccagt gcaccgaatg gttcggctac agaataccct gtaattctgt
    20941 gttcactcaa tgcggctggc atttctgctg gcgacatttc atgatagcta aaatgccccg
    21001 gtttaatctt taattgttta cgtaattcct caagtaaaag ataatgtgtt gaataacgat
    21061 gtggtatacc aaaatggtaa tcatcgccat tattattaaa ttcatttaag tgcatacctt
    21121 tttgtcccat aatgacattg ccttcatgat ggcccaatgc cacagccttt atatttgagc
    21181 ccttctgttt tgatttcatc gctagctcta ttaaagttga tgcaccatca atacgaccac
    21241 tgtttaatgc gtccattaaa tctggccaat tattgaattt aactaattct agtttatatt
    21301 tcggatgatt gtattgtgat aataattttt tagtcatcat caaattagct gaatgtgtaa
    21361 tcggcaaata tccaatttta atcacttgct gattttgggc atttttagac cgttctttag
    21421 acgtcctttg ccaatcacat cctgtaatta taaagattcc aatgatgacg attatgctta
    21481 accttttcat cgtcactcac tccttataaa taatattcag gttcaacttg atgatgattc
    21541 aatgcaaatg tttccataat ttcattacga atcttaagta ggtggctatc attacgactg
    21601 cgtggatgtg atgctgtaat ttcatattga gaaataatat tgcacccttc acctaacaga
    21661 acaatgcggt cggaaagata aatagcttca tcaatgtcat gcgtcactaa aataatagtt
    21721 gattgcgttt tatgttttag ttgcactagt tgatcctgaa gtttataacg tgtaaatgca
    21781 tctaatgcac ctaatggctc atccatcgat ataacgttag gcttatgcac atgcgctcga
    21841 catagtgcca cacgttgttt catacccccg gacagttgct cgggaaaatg ctttcccctg
    21901 tcttctaaat caactaattt aagctgtgcg ttaatctctt catcactaat tttctgttgt
    21961 aatccaatcc taatgttgtc attaatcgtt ttccatggca gcaaattatg atgttgaaat
    22021 agcattaaac aatctggaga tggctgttgt ttaatttcgt tatcaataat gacacgacca
    22081 gacgatggat gaataaatcc accgataata ttgagtaaag tagactttcc gcaaccactt
    22141 ttccctatga aagtgactat ttctcccttg ctaatgtcca aattaaagtt atgaattact
    22201 ttatgtgatc caaagtgatg ttgtaattgt tgaattttaa tcatgacaag ccccctttta
    22261 acccctacta ttttaatagg aattaagtgt atatctaaag ggctatgcca aacacagccc
    22321 ttatatcgtg aattgttata tctatagatg attatggttt ggtatgagtg ctcttataac
    22381 ataagcgtta ttcaattaac gacgttatat taatttcagc tctactaaat ttcaataaaa
    22441 tgattgtttt agacaatact tgcatttagt tcattagttt cttcatatca actacttaca
    22501 tttatagcat tgagctattc taattaactg caatcgaaat ccatgccaaa cctatataca
    22561 tatatctcat caacttgttg tcttattaaa gtttttcgat gccatcaatc ggtgtatcaa
    22621 ctgctttgcg cgcgcgacgt accgcttctt catcaggtgc gttgtataaa caaatacatt
    22681 tagacatatc ttcacataca tatgtgcgtt taaattcaac ttctggcact tcttcataat
    22741 gaacagaatt tttcttttta cgtgctaaat attgatccat cgtaatgcct tccggaatgt
    22801 tccaagttac aaggtaatca acatgtgcat caccattttt aacttcatct aaatctttac
    22861 caactaagcg tacttctttg actaattgaa tactaatatc tgcttctgtt aaaaattgtt
    22921 tagcttctgc ttcgtcattt gcttccacaa taaaataacc atgagttaaa ttttcagtag
    22981 cttgtacttc aattaatgtt ggaattgtct tcgtagatag tgatgctgcc tttgcttcta
    23041 gttcttcttt cgttgatgca aaatcaagat tgttagcttc taataaaaat aatgtcatta
    23101 ataagacctc ctataacaat ggttgaaaaa atattactat aattcttatc ggattacaag
    23161 gatattaaga tattttttta aatatttaga taaaattctg taaaatatat tttcaaagag
    23221 tataaaaatc atgttagggt ataagtaaat aaacatatat atagggggct atcattatgg
    23281 ataaagaatc tgtcgtagca agtttagcta gaaacaaaaa gattgctgta gaaacaatgg
    23341 ccggtcaaag gtacatcata gaacgcattt tacatacaaa tgatgaaaaa catattcata
    23401 ttttgaaacc taaagatgtc gtgttagatg tagatagcat caaagaaatt gacgaaaatc
    23461 atttaaatga tgcaacataa gtataaaacg aaacgtgcgc ctatgacata gattaatgtt
    23521 ttaggcgcta caatgtttta tttatgttaa tttgtcgaag tatggctcat ctaatggatt
    23581 caattgcaag ttttcaattt gcaacgttga atgtgcaatg ccatattttt ctttcaacaa
    23641 tgatgatact tgatcaatcg cttgataatc atcaccctca tattttttat ctaacacaac
    23701 atgggcactt aatgaataat gctctgttgt aatactccac aaatgaaatt catgtacatc
    23761 taatatgcca tctatgtttt taatatctgc cataatttga tcagtatcca aatgttgagg
    23821 cacactttcc attaaaatta accacgcatt acgcgtaatt ttataaccac cacgtaaaat
    23881 gatgagtgaa attacaatac taatgattgg gtcgatgatg cgccatcctg taaagtaaat
    23941 caatacaact gcaacgatga caccaataga gttcaataag tctcccatga aatgccataa
    24001 tgcactttga atattgatat tgtcttcttg ttttaaagac cttacaagga taacagtcaa
    24061 aataatattg acgagtaaac caatactagc aatcataaac ataatgccac tttcaattgg
    24121 ttgcggataa ataatacgta caatagcttc atataaaatc cagattgaaa ttacaattaa
    24181 tgctaaacca tttaaaaatg cagctaatat ctcaaatctt aaatatccaa atgtgtatcg
    24241 tgcagtcggc tttttacttg caaaataaat ggccaacata gataaaccaa gtgctaatac
    24301 atcactaagc atatgaaatg aatctgacag taatgccaat gaattagata ctaaacctcc
    24361 gacaaattca atcactgtaa ataacaatgt gatgattagt gatgcccaca gcgtcttttt
    24421 agaactttgt tgaatttttt gatgattgac atgatgaaaa tatttgtatc tgtttatcgt
    24481 attcgccttt ttcccattca tacgtttgcc actcctaaat aaatgcgtcg tatttaatat
    24541 tgtcgctttt aaaaataatt ctttatagct tctcaggtta atattaatgc ttccgtaatt
    24601 aaccatgatt tatacatcat cgtcatttac atgacaactc attaatcttt accctttata
    24661 gctttatgta atatcacctt ggaaaacttt tacagcacac tcgcgctttg ctttattcct
    24721 atcatataga aaattttaat aatgtacgag attattgtgt tagttacata aaaatgacaa
    24781 aaaagtatct caaatttatt gtttagtttc attaagttca aaaaatcgca ataactttaa
    24841 tgattgttcc gcatattaaa acaaacgatg actttgcata ttgaacttca tatttctaaa
    24901 tcaccataat taataaacaa ctctgtcctt ataatagccg catcgcaaat tagcataatt
    24961 aaaaacagca gtaagatgat ttgcaattaa aaaccatcct actgctatta ttttagtcat
    25021 tatatcctaa tatcatgata cgtgttccta tatattttgt tttgcttaaa atctaaacaa
    25081 cgccgttaaa tgttaagact taccgctaga ctgacctaca aacacgcttc agaaacgata
    25141 tttattctag tacaaacctt ttaaagcatt gcttgtatca atataaatat ttttaacttg
    25201 ttgatagtta cttaacgcac ctttataagt ttctcgaccg atacctgatt ttttataacc
    25261 accaaatggt gcgccttctg gtacttggtt gtaagtgtta atccaaatac gtcctgtacg
    25321 tacagcttta gcaatattta atgcacgtgt gatattttga gaaaatacac cgcctgctaa
    25381 accatactca gaatcattag ctatatcaat tgcttcttga tcgtccttca ctttaatcac
    25441 tgttaacact ggtccaaata tttcttcttg tgctaatttg tgatgattgt ctggcacagc
    25501 aattaatgtc ggctcaaaga agaacccttt atctaatcca ttttcagtta agcgatgacc
    25561 gcctgctaaa atttgtgcat ctgattcttt tgctgcatca atatatgatt gaattttatc
    25621 taattgatcc ttaccagttt gactacccat ttgtgtagct tcatcttgtg gatttccaac
    25681 tttaatattt gaaaatgcct cttgtaaacg tggcaccaat tgatcataaa ttttttcatg
    25741 aactaataat cgagaacctg cactacatac ttcaccttgg ttgaataaaa tacctaactg
    25801 aataccttca actgcaaggt ctaaattagc atcatctaat atgatattgg cgcttttacc
    25861 accaagctct aatgtagcgg gtactagatg ttttgctgca gcttcggcaa cttgataacc
    25921 tacatcagtt gagcccgtaa atgataattt atctacacca tcatgattga aaattgcatt
    25981 acctgattct gaacctttac ccgttagtat attgacaaca cctttaggta atacctcttg
    26041 gaaaatttta gcaacttcca ataaacttaa tggtgttgaa gacgaaggtt gaatcacaat
    26101 tgtattacct gcagcaatgg ctggcgcaat cttccatgca gctaatagca ttgggaagtt
    26161 ccaagcaaca acagcaccta cgacgccaat cggctcatgt cgtacgatac tcattgtgtc
    26221 tttatcgata tcattcactg taccttcttc tgtttcaata acacttgcga aataatggaa
    26281 atgtcttgca gcaaatggaa tatcaattgc tgttgtctca cgaatcggtt taccattatt
    26341 taatgtttca atcattgcaa ttttatcttt ttgtgccatt aatttatcac caatatcacg
    26401 caacatttgt gcacgttctg atttagaagt taatgaccat gattcaaatg cctcttgcgc
    26461 cactttgact gcatgatcga catctttatc ttttgctctt gtaatatgtg atagtgtttc
    26521 tccagttgct ggattagtca cttcgattgt ttcgtcactg ctacctttaa caaattcccc
    26581 attgataaat aaaccataat tctctgcaat ataatctcga acgtttactg ccatattatc
    26641 tctcctttgt ttattggcta taatataaat ctcttagcat catatttaat aatgctttta
    26701 aacgatatga ttcacattat atagtaagcg tttacatttt ataaaataat cagctcacag
    26761 aagtctcatt tttaaaactt ttgcgtcgtt tttgcacata aaaataatct tattttcgaa
    26821 aacatattgc acttttcaaa aataaaacac ataatataac tataatactt gagaataatt
    26881 ctcattaaat agtttggttg ttatgaaagt cgcgcttaaa atgcgttaat gtgacaagga
    26941 taattcaaca tcgtttcatt ttactgagtc attgcactta tcatacacat tatatttagc
    27001 atgagttata ttactaaaac aatattacat gcgcattagt actagaatgg agttttttca
    27061 atgcgactta ttttaatcgt cattggctta atatttacag cgcttggtat tgcaggtgcc
    27121 gtattacctt tactgccaac gacccctttt ttactcgtag cagttttttg ttttgctcga
    27181 agttcagatc gcttttacaa ttggcttatt aatcaaaaaa tttataaaga atatgtagaa
    27241 aacttttatt tacatcgagg ctacacgcta caacagaaaa ttaaaatttt aattagctta
    27301 tacattgtaa taggtttttc aatttatatg gtggatgttc ttgcagtccg tgtaggatta
    27361 atcataatgg ttatcataca aaccgctgta ctctttacat ttgtaaaaac attacccaaa
    27421 tcaaatcata aaatagagga gtgattgccc atgtttatgg cagaaaatag attacaatta
    27481 caaaaaggca gtgcggaaga aacgattgaa cgtttttaca atagacaagg tattgaaact
    27541 attgaaggct tccaacaaat gtttgtcact aaaacattaa ataccgagga tacagacgaa
    27601 gttaaaatct taactatttg ggaatctgaa gatagcttta ataattggtt gaattccgat
    27661 gtatttaaag aagctcataa aaatgtacgt ttaaaaagtg atgacgatgg acagcaaagt
    27721 ccaatattat caaataaagt attcaaatat gatattggct accactatca aaaataaata
    27781 tattaaaaac ccctagtcac cgctggttgt ctaaaccaac ttcatgatta ggggtttttt
    27841 attatttgtg acgtaaaggt acgaattcat ccggcttgtc tgtgcgcaat ccaaaataat
    27901 attctattgc ttcacaaata cgtcgtgatg cttgtccatc accatatgga ttattcgctt
    27961 gactcatacg ttgatacgct tcatcatcct caatcaattg tttcacattt cgaacaatat
    28021 tgtcataatc tgtgccaatt actctcgatg ttcccgcctc aacgccttcg ggacgctctg
    28081 tatgattcct taataccaac acaggttttc caaatgtagg agcctcctct tgaataccac
    28141 cagaatctgt cagcacgagg tacgattgat ttgtaaaatt atggaactca atcgcatcta
    28201 atggctcaat taattcaatc cgatttctcc cagataaata tttttcggca atcgctctta
    28261 cctttggatt acgatgcatt ggataaatga agacaacatc tttatattca tctgccaaat
    28321 ctcttactgc tttaaaaatc tgatgcatcg gttccccaat attttcacga cgatgcgctg
    28381 ttagtaaaac aactttcttg cctttatgtt tattaataat cgttgaaaca aaatcatttt
    28441 gaactgttgt tgacaatgcg tcaataactg tatttccagt aataaagata cgctctttgt
    28501 ctttgttttc aaaaagtaaa tttttagctg caattactgt tggcgcaaaa ttcaattcag
    28561 caatattact taccatgact cgatttaact cttcaggaaa tggtgagtat ttctgatgtg
    28621 ttcgaagtcc agcttctaca tgtccgaccg gaatttgatg ataaaatgct gccaaacttc
    28681 ctacaaaagt cgttgtagta tcaccatgta ctaaaatcat atccggttgt tcctcattaa
    28741 tgatgctatc aagtttagca agtgcattcg ccgtaaggcc tgctaatgtt tgttgatctt
    28801 gcataatatt taaatcatga tcagcttgaa tatcaaatat acttaacaca ctatctaaca
    28861 tatctctatg ttgtgctgta atcacaatgt tcgcttcaaa gttcccatta tgatcaattt
    28921 cttttactaa tggtgccatt tttattgctt cgggtctcgt accgaaaata accataattt
    28981 ttttcataac attatcctct ctgaagttca aacacatgac agcttatctt tccttaatca
    29041 tgtatgccct agtttgacac attttatttg tcgataaaat taaatatatt gccataatta
    29101 taatacgata catcttcaaa tgatgatttc acaacatttt ttgtatcaaa aatcacttta
    29161 tgcttcattt tatcaaaatg actgtccgat aaatttttaa attctgagtg gtcacttaaa
    29221 atcaatacta gcgatgcgtc tttgacagca tgtgacatat catgttccac aaaatctaat
    29281 tcaacatgtg gatcataagc acatacttct atgtctggtt cttgatttaa tagctcataa
    29341 atatcaaatg ctggtgattc tcttatatca tcaacatcac ctttataagt taaaccaaat
    29401 actgtgactt tattcccgct caacactttg atgatttgct tcgttgtatc aacaacatag
    29461 gccggcattg aattattaat ttcacgtcca gtttgaatta actttgcatt ttcagggtct
    29521 ttagcaataa taaagtacgg atcaacagct aaacaatgac cgcctacacc tggaccaggc
    29581 tgatggatgt taacacgcgg atgtttgttt gccatttcaa tcacatctaa tacattaata
    29641 tttaagttat tgcaaatttt tgttaattca ttagctaaag caatgttcac gtctctatat
    29701 gtgttttcca ttagcttact catttcagca gtacgtgcat ctgtttcaat catttctccc
    29761 tgaacgaatg tgcgatagac acgtttaccc gcttcaatac aagcttcagt cacaccgcca
    29821 atgatacgat tgttatgaac taattcttct aaaatttttc ctggcagtac acgttctgga
    29881 caatgcacta aataaatatc ttcacctatt gtaaacccta aattttcaat gactggtttt
    29941 acaaaatcat ccatcgtttt aggcgcaatt gtcgactcta caataatggt atttcctttt
    30001 tctaaaaatg ataaaatact atctaatgca cgcataacta gcgaaatgtc acatgaccgg
    30061 tactgatcat cattattcgg cgtcggaacg gcaatgataa aaacatcaga tgcatctggc
    30121 gttgtagata ccttcaattt tcccgatgac agtacctctt cataaacctc ttgtaatcca
    30181 ggttcttcaa tactaatttg accactttgt aacttatcaa tcgtttgctg attaatatca
    30241 acaccaagca catcgacgcc atgttttgca aacataattg atgttggtaa accaatataa
    30301 cctaagccaa ctactgttaa cttcatacta taacctccaa ataaaacgat ttgtatgttg
    30361 attttattta atagatgact tatgccttat ctttggttgt cgtttcatcc atgatgtccg
    30421 caataacaag tgacattttt ccaggaataa tttcaagtgc attattattt tcatataacg
    30481 tattgctgta tattaaattg ccgaataatt ttctaaagac cgacaactta ttaaaatact
    30541 tatttagcat tgaaggcatg ttgatcaata ccgttttacg atgtgattgg cgacgtattt
    30601 catacattac tgacgatgta tcaaagtaaa aactatcttg aggatggtac acacctgtca
    30661 cttctaatga tattaattga tcaataaatg ctgtcagatg tttaatatat aatgcactgc
    30721 gctgattgtt aatattggga atgattggca atcgctttga caattgcatt aaccgttgga
    30781 aatttcctgg gcaatgtgca ccataaatca ttggtggtct cacaattgct actttaaacg
    30841 aatcactaat caattcttgt aatgcttgtt cagcgaactt tttggaaata ccatagttgg
    30901 tcgtagggtt cattggtgtt tgtgtatcaa cttgatctga tttaccaaca tgaccttctt
    30961 ttccataaac tgccatagta ctcataaaaa taaattgttt aacgtcttca gctttagcct
    31021 tttgtgccaa ttgtttcgtc agcaacatat tcacttgcat ataatcagat agccttgctt
    31081 gaggtgaatt gttgtgaacc aaagctgctg tatgaattaa aacatcataa tctttgaacg
    31141 aggtcgactt ccataattga ttcctaacat taatttgatc tacttgatgt ccttgttcaa
    31201 taagcttatc ttttaaagca ttaccgatat atccatgtac gcctgtaatt aaaatatttt
    31261 ttctcattag tgatgcacac cttctgaagt aacgatattt ttaattgttt tatatatgat
    31321 atacatatca agcatcatag attgatgtgt taagtaataa tgatcatacg ctactttttg
    31381 atcatcagtg atatcatctc tccccatcac ttgagctagt cctgtcacac ctggtctaat
    31441 cgtatgcacg ttcgcttttg tacgtttttc gattaattcg tattgattat aaagcgctgg
    31501 tctaggacct acaattgaca tttctccttt taaaacattc aataattgtg gcaattcatc
    31561 aatagaggtc ttacgaatga ccttccctgt ctttgttata tacgatgttg aatccattaa
    31621 atcagttgca acattaggtg tgtctatttt cattgatcta aacttataaa tattaaacaa
    31681 ttcattatta atcgtcggtc ttttttgttt gaaaatggct ggtccaggtg attccatttt
    31741 aattagtaat gctgtaatta acagaatcgg acttaaaact actaaaccat atattgaact
    31801 cactacatcg aataatcgct tcataattat ctcctcactt caacagattg taatacttca
    31861 tagtattaat ttctctcgta tatttatctt taaacagttg atgtgcgcgc tcacccatct
    31921 cgtgcaattc cttactcgag tgagtgttga tgaagttata aatggcatgt gcatcgccat
    31981 tatcaatttg gatacccgca tcatattgtt caacatgttg aacgatatca gattgcttat
    32041 ccatgatgag taccaacgct ttcttagctg caagatagcc ataattcttg ctcggcacgc
    32101 ctaaaccgac gccttcttta atcagcgatg caatacatac atccgcaatt tttaatacgt
    32161 cagcatagtc tgtacctgtt aaaaactcaa acattttaac attttcaata cgatggtctt
    32221 ctattgccgt tttgacatct gcaaatttct taccatgacc acaaagtatt gttaacgttt
    32281 gagactgatc cttatttaat tttaaaaatg agataagtgt ctccatatcc tgtaactgcc
    32341 ccatattacc gctatacaat aaaattttgt cgtattgctc acggtaagct ttaaatgtgt
    32401 cattatagat acgattgtct tgtaattgac gcatgtcata ccagttagga atcacatgga
    32461 tattgtcagc atttttagaa atttgatgat ttagtaagta gtttttcatt tccgtaccaa
    32521 ggacaatgac attttcagca ttcttgtaga catgtctatt aatgtaacgc atcagcttat
    32581 caatcatgct acctggacga gttgcacctg tcttaatcgc attatcaggt gctatatcat
    32641 acaccacaaa agaatatttt ttcttaagca gtctgtgtaa aacgtctggt attaatggca
    32701 agattggtgg attagagtaa acaagaatct gatcatattt caacatttta ggtatattaa
    32761 tcacgaattt tgaaaataaa ctaaagaaat tgatgatcct tccaacctta cttttgttat
    32821 taaacctcga atacttgaga cgtcgaatgc gaataccacg atgcatctcg gttttagaaa
    32881 cctgtttatg attactatat tcatatggcc atccacacat gacatcgaca ttaatgtgat
    32941 tcgcaattaa atcttccgcc aattgagttg gtaacgtcgc agaagataca tattccggat
    33001 aaaaatactg acataaaatc aaaatctttt tttcactcat ctaatcgacg tcctttttat
    33061 taatgaattt agactttaaa cctataatat ttaaaatcac atacttaata atgttgctga
    33121 acagcgttgt ccaaaagaat ccatttagac caaacgcaat tgtcattaaa atagttatga
    33181 atataaatgt aatcgtgtga agcgtcatat aattcgcttg taatgttatt gatgcgtgtt
    33241 ttgtatttaa agtttggatc ataatactca ctgcattgaa taaaacacct atattaccta
    33301 aaataataaa cttcgaatat aaacttgaat caatgttata cagtaaagag acaataatta
    33361 ttgtaattgg ataacatata atcatgacta aacatagggc agctatagca attagattag
    33421 ttttcaaata ttgcttcttt atattgtcac tttcatttac agaaatatat gaaagtacta
    33481 cattattaat cggatataga aatgtagcta acattttccc aataaatgtt gaaagaaatg
    33541 atatagttac agctgttcca cctataattg gtaataagat taatctatct aaatagagat
    33601 tcaaattatt aaggctattt gtactcagta gcatcacata atcttttacc acattattat
    33661 cttcactttg atactcgcct atagttaatc cccgtaattt aaccaatgta tatatcgttg
    33721 caaacaattc actggtaata aaacaaacaa tccagttttg ggttaaataa tatagaaata
    33781 gtcctatcag caaacctaaa aattgaataa gagcaatata caaaatctga ttatatttta
    33841 aagtcatcct aaaaaataca ttcagataaa tccttaaaca cattaaaata ttaagtagaa
    33901 ttaaaaagat aatatcgatg atgttcaaat taaaaaagta aagaaataca ataattaaag
    33961 ctatactctc aatcagaatt gagattaaaa gtatcgatgc aaatttccag tagtaatgat
    34021 tggatttgta tagattcata ttaatcaatc gtatattgtt aagcgtattg cctaatacaa
    34081 cactcgtgat tgttattatt gtataaatcg ttaaaataga accgaacgct tcattaccta
    34141 ctcgttgatt aataattgga taagctaaaa attgtaatcc taaagctata agcaacgtac
    34201 caattattgt ctttacgcta tccataataa aaactttctt agccatgatg acgccgtcct
    34261 tttgtaataa aatagaacaa cacaaaccaa aattcactta caactaaata atttgaacta
    34321 aacattaata atgtgattgg atagattgct ataaacataa cgagtaaatc tatagtgttt
    34381 ggatcatagt tcctaatcat tttataaact agtagcaaaa tgcaaatcat tatgataaaa
    34441 aaccctaata agccaaatga tagaatcaac tcaataatga tgttatgtgg tatatttccg
    34501 attagtttat aatagttaaa tggcccatag cctaataacg aactttgttg aataaagtaa
    34561 ataccttttt cataaatcgg tcctcttcca gaagtacctt ctaaattaag tgttccacct
    34621 tgtagatatg aaaatgttct agtattcgaa ccttttgtaa aaagaaagta aatcaataca
    34681 ctagatatgc ttaatgcaaa aatatacata atgcttttta ctgcgatagg tattcctctt
    34741 ttaaacgtaa taagtataaa tgcaaataag ccgtaaagaa ttaataaaat agcacctcca
    34801 cgccctcctg gtataaacac aatagggata tcaattattg taaatagaac atatatccac
    34861 ttatgtttaa ctgaaccttt cataatgaaa taaatgccta atccggcagt aaatgctgaa
    34921 aggtacgaag cgttttgata gttcataagt ccaaaattga tatagctagg tatctcacct
    34981 gtaagttttg gtattaaaat tacaaaaata aatgaaacag aaaatatgaa aaatactaat
    35041 ttaaaaaatc tttctaccgt agccttgttt atatatttaa tataaatacc actaattgcc
    35101 gctggaactg cccatgttaa aaagaataga atattatttt tagctagttt ctcttcctta
    35161 tctggtgaaa aataataaaa agctagataa agcaaacata tagcaattaa taatattaac
    35221 cctcgcggaa tttcttgggt gacaattatc ttataaatag caaacaccgt agtaattaat
    35281 gctataccaa ccatggttga gtaatacacc ggctctatag ggaaccctaa tacttcttta
    35341 gtaaatgtag agattactat aaatatgttc atgctgataa ttgcacaaag tacaaaaaat
    35401 ttcatagtct acctctcttt aaaatttata taattctact aaccgttcac tctctatttg
    35461 ccaattcaaa attttagacg ccttaattgc attttgacgt aaatgattaa acaaatcgtg
    35521 attatctctt aattttctaa ccgccttttc aatttctaac ggcgtaactt cctttagaac
    35581 aatgccaaat ttatattttt cattgagata aatatgctct ttgacaggag ataaaattac
    35641 tggtaaacca gcatgtatac attcaaaaat tttattagat actgtatatt caaaattaat
    35701 agatactggt ttcgtcaaga caacaccaac attactttct gctaacttat caaccaattc
    35761 ttttacttca actggtttat ccaacctaat attttccggg ttataactaa tcagttcttt
    35821 tatcacttct tcatgcggac caaaccctcg aattatgaat gaaggagcat tttgtttaaa
    35881 agctgatgaa gcaataataa actcttcata tcctctgtcc attacaattt gaccttgata
    35941 tacaatttct ttaaagtttt cgatttcttt aaattctctg ctatcattta aaataggtgc
    36001 attcgtaata acattcgctt ccttcttata tcctttagat tgataatatt cttttgctgc
    36061 atgacttact gttacgaagg cattaacacg atgttttact atgtgttttt ctatactttc
    36121 tacaaacttt gaaataagtg gaactttatt aataaaggca tttttcgcat atatttcatg
    36181 cgcatcataa acaatattag cttttttata attgcttaaa tagaccatta ataatacgtc
    36241 gaaatcattt gcatgaatca cgtcaggttt ataagcttta atttctcgga taacacctgt
    36301 tgcaaatctt atacgcttaa ttaatttaga aagaatattt tttggatcta ccttgctacc
    36361 taacaaacga taattacaat ctaaattttc caatcgctta ttagtagctt gtgaattatt
    36421 cattccaaca attttataat catccgtaac gcctttaatt gtttctattt gtttaagtac
    36481 ccttgggtct tgaacaatat tactcgatac aatatttaaa attctcatat cattaagtat
    36541 cctttttatt taaatattca accgaaaatc cttttagttt gtcaggcgtt ttctcccacc
    36601 acttgctttc caaaagtttt tcaattgttt tattgtcaaa tcgcttctta atcacttttg
    36661 caggaacacc accaacaacc tcatatgctc ctacattttt agtaacaact gagccggctg
    36721 ctatgactgc accagtattt attgttaaac catccataat aattacattt gcaccaatcc
    36781 acacatcatt tttaattgtt gtacggcttg gttggtcatt aaagtctata aacttttgct
    36841 ttatgttaaa tggattatta ttagaataaa aaatcggtga tgagctaaaa aagtgtgtag
    36901 gatgttttcc taacccaatt tttacatccg aagatatcga acaatatctt cctacttcta
    36961 cattattaaa atcactacca aatccaatat aactgtattc accaatgtga gaattcctga
    37021 ttttacacca tctatctata tagttattgc catcaaattt tgagtttgta atatacgcca
    37081 agcgatgaat cttaacattc gattctttag aggactggtt tttcagcaaa ccaattatct
    37141 tttcaatcgc tatcctcatc gtcatttcct ccaagtattt cgattgataa tatctttata
    37201 gctttgaata attttaacta cctttgtcga aacgttagtg tctttataat caatagcatc
    37261 aatcatcggt tcgttattgt tttgcatctc tcttgctagt tcaacggatt ggattagatt
    37321 gttataggta ataccaccta caataaccgt acctttatct agtacttccg gtctttctgt
    37381 ggaagttcga ataaggacac cagggaactt caaaatagac gactcttctg acaatgttcc
    37441 actatctgat agcacaacaa atgcatcttt ttgcaatgca ttataatcaa agaaaccaaa
    37501 tggctttaac tgtttaacta atggatcaaa ttcaaattta ctttcttcaa ttttcttcca
    37561 acttcttgga tgcgttgaat aaatcacagg cattttatac tttttggcaa tatcatttat
    37621 cgcattcatt aatgatttaa aattcttttc attatcgata ttctcttctc tatgcgcaga
    37681 tactaaaatg tattgttgcg gttctaatcc tagtttattt aaaacgtcac tgtgattaat
    37741 tttatctcga tgcgcttcta tcacttctgt catcggtgat cctgtcacaa agatattcgc
    37801 tttattgagg ccttcatcta ataaataacg tctgctatgt tccgtataag gtagattcac
    37861 atcactgaca tggtcaacaa ttttacgatt gatttcttca ggtacattct gatcaaagca
    37921 tctattaccc gcttccatgt ggaacacagg aatctttaat cgtttagcag atactgctgc
    37981 taaacaacta tttgtatcac caagaattaa aagtgcatct ggttgttcgc gtaataaaac
    38041 atcatatgtc ttcgcaataa tattccccat cgtttctcca aggttacttc caactgcctc
    38101 taagtagtgg tccggttgtc ttaattccaa atcatcaaag aaaatttgat tcaatgtata
    38161 atcataattt tgaccagtgt gtactaatat ctgattaaaa tattgatcac atgctttaat
    38221 cgttgatgat aaacgaatga tttcaggcct tgtaccaact attgtcatta atttcagttt
    38281 ttccatgcgc tatacctcca agaaatacgt atctggctga tttggatcaa acatttcatt
    38341 cacccacata atagttacca tatctgtgtc gcctaaattt tcaatattat gtgtgtatcc
    38401 tactggtatg tctacaactt ctaatttgtc gccagaaaca taatattcaa tgatttcatc
    38461 atcattaaca tgtctaaaac gaattacccc tttacctgat acgactagaa atttttcgtt
    38521 tttagtatga tgccagtgat tacctttagt aatacctggt ttagaaatat ttacagaaac
    38581 ttgaccacga tccggtgttt ttataaattc tgtaaaagaa cccctatcat ccacattcat
    38641 aagtaaggga taactaaagt ctgtactagg tagatagctt aaatacgtac tatacaaatc
    38701 tttttcaaac aagttatcta atttcggcaa tgttcgatcg agacgtgact gtttgaactt
    38761 gtataataaa tctacaattt ctcccaatgt cactttaaat acgtttggta ctgtaggtac
    38821 accattttca atcgttggag ttccttcaat agcacgcttt atttcagcga cgatatcatc
    38881 cacgtagttt agcgttagtt caacattccg atcattaact tgaatctctt cgttacgtgc
    38941 aattttgtaa caaaatgttg ctatcactga gttataattc ggcttacacc acttgccgaa
    39001 taaatttggc cagcgataaa tataaaccgt attgccatac tcttcggcat actctcttaa
    39061 tagctgttcc ccttgcaact tactctcacc ataaggatta tcttgtgttg cttgtattga
    39121 agacgataat aatatcgctg gctttttcgt atttctagtt aatatatcaa gtacatgatc
    39181 taaataactc acgtttccta agctgaattc tttgtcatgt tcaggtcgat taacacccgc
    39241 taaatgcacg acaaagtctg ctttcaacaa tgctgactct aattcttcct ctttagtttg
    39301 tcgatgtact tcgaaaatat gatgatctgt cgttgaagtt aaatctgctt tcaagttttt
    39361 tcctacaaaa ccttttgctc ctgtaattac aatattcaac gtcaatctct cctatctcat
    39421 tgaagcttta taatcattca attcgtttct aacatattct agtgttaaaa gtttttcttt
    39481 tatctcttcc accgttaaaa tatgtgtatt atcggagtta tattcataag attgcgtaat
    39541 cttttcgtta ccggtttcaa cataattact ataatttaaa tctctggagt ctgccggcac
    39601 tctaaaataa tcacccatat cttcacattg tgcgtattct tctctcgtca acaatgtttc
    39661 tgcttttttc tctccatgtc gcgtaccaat gatttcaatt gcattatcag cttcaaacaa
    39721 ttctaataat gcggtcgcaa gatcccctac cgttgagctt ggtgcttttt gaaccataat
    39781 atctcctgtc tctgcatgct taaatgcatg aacaactagt tctaccgcat cttctaagct
    39841 cattaaaaat cttgtcatat caggatctgt aatcgttaaa ggttctccag ctttgatttt
    39901 gtcgataaac aaaggtatta ctgatcctct tgaagccatc acattaccgt atcttgtacc
    39961 acaaataagc gtttgttcac tacgaatatt tcttgatttg gctacgaata ctttttccat
    40021 cattgctttt gaaataccca tagcattaat aggatacgct gccttatctg tagataaaca
    40081 tatgactttt ttaacatttt gatgaatagc actttgtaag acattttctg taccaataat
    40141 atttgtcttc actgcctcaa ctggaaagaa ttcacatgac ggcacttgtt ttaaagctgc
    40201 tgcatggaat acgtaataaa catctcgcat tgctgtttct acactttgac tatcacgcac
    40261 atcaccaatg tagaacttta attttgaatt attatatttt tttcgaatgt catcttgttt
    40321 tttctcatcg cgtgaaaaaa tacgaatttc tttaatatta gaatctaaaa actgtttcat
    40381 aacagcatta ccgaatgatc ctgtgccccc agtaattaat aaaattttgt catcgaacat
    40441 aattatctcc ctttttgcca ttggcatagt taataatttt ttctttacta aagtcattga
    40501 cgatgtcttg aataatcgct tcaacttcat tacatttcat atgttgtact ttgccacgat
    40561 aaattttttc aaatacttgt tcaggatgaa cctcatcttt attcataagc tcttcaaaca
    40621 ttttttcgcc gggtctaatc cctgtataag taatgcgtat gtcgtcttct tttttaccac
    40681 ttagcttaat taaattacgt gccaaatcta caattttcac tggttctccc atatctagca
    40741 caaatacttc gccaccttct gctaatgccc ctgcctgcaa aactagtcta gaagcttcag
    40801 gaattgtcat aaagtaacgt gtcatttcag gatgtgtcac agtaactggc ccaccttctt
    40861 caatttgact tttgaaaagt ggaatcacag atcctctcga tccaagtaca ttaccaaatc
    40921 tcactgcaac aaaatttgtt cgatgcgttt catcatttaa actttgaata atcatttctg
    40981 caattcgctt tgaagctccc atgacattag gcggattaac ggctttatcc gtagaaatca
    41041 taacgaattt ctttacctct gcatttttag cagcttcagc agtatttttc gtacctaaaa
    41101 tattattacg tactgcttct tcagggttgt cttccattaa cggcacgtgc ttgtgtgctg
    41161 ctgcatgata aactgcgtat ggtttatacg tttccataat ttcaaacata cgcgctctat
    41221 tttgcacatc cgctataata ggaacgatat caacattttt ttccgaagcg atttcgcaat
    41281 tcacgattga ttaaataaat actgttttca ccatggccaa gtagaataat acgttctgga
    41341 tagaaattac aaacttgtct acaaatttct gatcctattg aaccacctgc acccgtaact
    41401 aaaatagttt tattcgtcaa ttcatttgat atcatatcca tatctaattc aacaggatct
    41461 ctgcctagta aatcttctac ttcaactttt ttaagttggt tcacttctaa ctcaccagac
    41521 atgacgtctt ctatatttgg cattttcaat aactcaacgc catccatatg gcaaatatta
    41581 ttaatttctt tcaaacgctc ttgaccaata gttggaattg caatgatgat ttttttaatc
    41641 ttatatttcc tcactagttc tggaatatcc gcaattttac cttggacttt tacaccctca
    41701 gtaattgtga tattgcgttt atgttcgtca tcatcgactg ctaataccgg ttcaagtttc
    41761 atttcgtcac ttttcaacat ttgtctaatc agcattgaac ctgcttgacc agcaccaaca
    41821 actaaagttg gcttcttatt aaatgactta cctccaaggt atttccgata aatacgccaa
    41881 aataaccttg agccacctat taaaatcaag tgcatcatcc aagtaattaa atacaatcta
    41941 aaaaacggtc tattgcctgt aacaattgtc acgaccacca tcgtaataac gatagatgtc
    42001 gtcacagctt taacaattaa aatcaattca ctcacactgg catattccca cgctcgatga
    42061 tacatattaa aaataaatgc tgaaatatga tgcgatatga atagtgatat agctgccaat
    42121 attaataatt tgacagaata tgttttgaaa tacggttcta aaatgtaata acttacgaat
    42181 actgaaaatg tcactatcag tgaatcgatt aatgctagta ttaaaagccg caatttcaca
    42241 gataaatgtg ccataaaacc cctcatttgc tgtttataac ccaaaccatc ttttctgttt
    42301 ataatcttgt tgtggcattc gtttaggaat ttttttatca tcaacaacta acttcgcatt
    42361 actaataaat ccgttcatat cttcataata atcacgtaat ttcttatcat taaataagtc
    42421 tttcattaag aacggtctga tttctgtgtt atgcgcatct gaaccgatga aatgtgtcag
    42481 attgttttca atcatttgaa ttgctaattt tctaattttt ttaccggaaa tacccgctaa
    42541 tgacgccgtt gtcacttgac ttaaagcacc tttgttaatt aaatcgtata gtatgtcaag
    42601 gttttgactt attgctttat tccgctctgg atgtgcaata atcggtacaa agcctttact
    42661 ctgtaattcg aaaaataatt gatcagtata gtgtggaact tcatttgatg gaaattctat
    42721 tagtaaatag cgtgaatcat taataccgtt aataactttt cgatcaatat cattaaggat
    42781 ttgatcggta attcttattt cctgaccata ataaaacttt agatttagtg cttgtacttc
    42841 ctctaagctt tcaatatggt ttaaacatga tttcactttt tctataggtg tggtatatcg
    42901 aggatgtaag tgatgtgatg ttacaatgat ttctgtaaca ccttgtgttg tcgcttgttt
    42961 taaaagatcc atcatctctg tttcatttgt cggaccgtca tcgatattag gcaatatatg
    43021 gttatgaata tcaatcatac ttattcatct ccataatagt gataataact agaagattta
    43081 tcgacctttg tcttgttcaa aatgacacct agaatgttac tgcctgcttt ttccataagt
    43141 gcttttgctt ttttaacttc atttttatca tttttttcac tatcaattac taacagacta
    43201 tctttaatag cacgcgcata tagttgtgca tcagtcacag tattaactgg cggtgtatcg
    43261 acaataataa tgtcgtaacg tttattaaac agatcaacta attctttgaa cctttcagac
    43321 ccaattaact cagatggatt tggaggtaca gggccagctg ttagcaaatc taaattttca
    43381 atttctgtcg acgtaattgc ttctgacata gtcgttcgac caatgattaa gcttgatagt
    43441 ccattattat tttgctcatt aaaaatatag ttttgtgttg gcttacgcat atcgccatca
    43501 ataactaatg tcttatagcc tgcttgtgca taagtaatcg ctacattcga tacaactgta
    43561 cttttacctg caccaggctt ttcagaagta accaataagc gctttacttc accatttgct
    43621 tttgaaaaca tgatgtttga acgtatacct cgaaactttt cactaattgt tgattttggt
    43681 ttttcatata caaatagtgt tgttgtcgta ttttcctttt ttgacataag tagcaaccat
    43741 ccttaattaa atttttgaat tgaacccaat acaggcaatc ctaattcttt ctctacatct
    43801 tcttcatctt taattcgctt atcgaaaatt actttgaaga agatatatat aagcgcgaca
    43861 actaatccta aaaagaatgc accgattaga ttcactacag tttttggtgc gactttaact
    43921 gctgtaccgt ctgctttaga taaaattgat acgttatcca cactcataat cttcggaatt
    43981 tgtttacttg taactttagc gaagctattc gcaatttttt ccgaatcttg tttatgacca
    44041 cttttaactt ggatgttgat aagttgcgta ttttcttggt ttgtaattgt caacatactc
    44101 gacaatttag atggtgaata cttatcattt aagtcctttg acacctcatc taaaattcta
    44161 ggacttttaa caatttcttt atacgtattt acaagttgaa tattactttg aacctcttgc
    44221 gccataaact gaggattgtc acccttagtt tgattcacta aaatttgagt attagcttga
    44281 tatttaggtg ataagacgaa aaatgtaaca atagcgctaa taattaaaaa taatagcggt
    44341 aaaataatta aaatcttcaa gtttttttgt aatacttctt taatttttgt taattctaat
    44401 gtactttcca ttatttacct cccttaaaaa ttttcattaa aattggtatt atatatatag
    44461 tatttacata ttacatatcg tttgaacaat taattacttt taaaccgcat tattttcgcc
    44521 tttttattta tatgaaatta aaaataatta tactctaaag tagtaatagt ttgtgaaatg
    44581 agtatcaatc ttaatatttt ctttatttaa aaaaatgatt gtctagtttg tatctctctg
    44641 aagatttggc aataaataaa agccgataac cgtataatga ttatcgactt aaagtttatg
    44701 tggcattttt tacttttgta atttcaggtg agttagatga ttattatcag atagattatt
    44761 gcttataatc atatgatgtt tgaatgatat ctttgatttc actgattagt gcttctttag
    44821 gattagcagt tgtacattga tcttcaaatg cgagctctgc cattctatca attgactcat
    44881 ttaattcttc ttcagacaca ccttgtgatt tcaaattcat ttcaattccg actgattgac
    44941 ctaattcgta gacagcttta gctaatgatt ctacgagtgc ttctgtcgta ttacctttta
    45001 atcctaagaa tttggcaata tctgcataat ctgtatctgc tctgaagaac tcatatttag
    45061 ggaataatgc atgtttttgc gggtctttgg cattataacg gataatatgc ggtagtaata
    45121 tcgcattcgc tctaccatgc ggaataccat attcgccacc aattttatgc gcaattgagt
    45181 gtgcaatgcc taagaatgca tttgcaaatg ccataccagc caaagttgat gcgttatgca
    45241 ttttctctct tgaaacttta tcaccctttt caacagatga ttttaaatat tcgaacgtca
    45301 atttaatcgc ttgtagactc aaacctcttg tgtagtctga agccattaca gatacatatg
    45361 attccattgc atgcgttagt acatccattc ctgtatctgc tgtaacgctt tttggcacac
    45421 tcatcacaaa ttgagggtca ataattgcaa cgtcaggtgt taaagcaaaa tcagccaacg
    45481 gatattttac atttgtttca ctatctgtga taactgcaaa tggtgttact tctgaacctg
    45541 tacctgatgt cgtagggata caaatgaacg tcgcattttc aggcatgcct attttataag
    45601 tacgtttacc gatgtctagg aacttttgtt tagcaccgaa gaatgatgtc tcagggtgtt
    45661 caaagaacat ccacattgct tttgcagcat ccatcgctga accaccacca agtgcaatga
    45721 ttgtatccgg ttggaaatca accatcattt ccagaccttt atatactgta ttagttgatg
    45781 ggttcggttc gacttcgcta aatattttaa tttgaggctg ttccgttttt tgtcttaata
    45841 cattctcaac tgtttttgta taaccgaatt ctaccatacc agggtcacag acaatcatca
    45901 ctttttcaat cttgtccatt gttgttagac tcatgattgc attttcttca aaataaattt
    45961 gagcaggcac cttgaaaatt tgagtattat tacgtcgttt agcaatcgtt ttaatgttta
    46021 ataaatctgt cgcactaaca ttatgtgaaa ttgagtttct accgtaggaa ccacaaccta
    46081 atgttaaaga cggaatcaat tcgttataca tatcaccaat acctccaacc gctgatggtg
    46141 tatttacaag tacacgacaa gctttcattc ttagtccaaa atctttttgt aatgtttcat
    46201 cttctgtatg gataacggct gtgtgtccta atccaccaaa atgtagtgtg tcttcacaaa
    46261 tttgaaatgc ttgttttgta gattgggctt ttactaaggc taatactgga gataattttt
    46321 cacgagataa cggatagtct gaacctacac cgctaatttc ggctatgata agttttgtat
    46381 tttcggggac aggtatacct gctaattcag ctatttcaac tgcagattta ccgacaatat
    46441 caggcttaat acctgttttt tgttcattca taattgcatt ttctaagcgt tgtaattcat
    46501 cttttttaac aaagtatgct tgatgtgctt taaattcatt agtaacatct ttataaattt
    46561 ctttatcaat gactacaact tgttcagaag cacaaatcat accattatca aatgtttttg
    46621 aaccaatgat atcatttact gcacgtttaa tgtgtgctgt tttttcaatg taagacggca
    46681 cgttacctgg tcccacacct aatgccggtt tgccagttga atatgcagac ttaaccatgc
    46741 ccgaaccacc tgttgctaga actaatgcaa tacctttgtg attcattaat tgttttgttg
    46801 cttcgataga aggcacttca atccactgaa taatatcttt aggtgcacct gccttcattg
    46861 ccgcttctaa tacaacttct gctgcacgct tcgacgattc ttgtgcactt ggatggaatg
    46921 caaaaatgat tggatttcct gtcttaattg caatcatcgc tttaaaaata gttgtcgacg
    46981 taggatttgt tgttggcgta acaccacaaa taacaccaat tggttccgct acatacgtta
    47041 atcctttttc tttatcttca ccaataatcc ctactgtctt attgtctttt attgaattcc
    47101 atatatattc agaagcgtat aaatttttaa tcgctttatc ttcgtatata cctcttccag
    47161 tttcttcatg tgctaatttt gctagcacca tatgttgatc aacagctgct aagctcattt
    47221 gatgaacaat atgatcaatt tcttcttgtg actttttaga taatgcttct aatgcttttt
    47281 tccctttgtc agctagagca tcaatcataa ttgccacttc ttgttctttc gatccacgat
    47341 tttctttttc aggtatagtt aacatataca accactcctt tatactttgt gaattatttc
    47401 acaaacatta tagtacatgt ctctcaggat ataaagaaaa ttctatacaa aaaagtttaa
    47461 tttcgaatat tatttgaaca aata
//
LOCUS       DALAAR010000012        46321 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000012 DALAAR010000000
VERSION     DALAAR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 46321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.012"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(555..1001)
                     /gene="perR"
                     /locus_tag="O2458_000675"
     CDS             complement(555..1001)
                     /gene="perR"
                     /locus_tag="O2458_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCZ3886559.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1098..2048)
                     /locus_tag="O2458_000676"
     CDS             complement(1098..2048)
                     /locus_tag="O2458_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ3886560.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(2054..2509)
                     /gene="bcp"
                     /locus_tag="O2458_000677"
     CDS             complement(2054..2509)
                     /gene="bcp"
                     /locus_tag="O2458_000677"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCZ3886561.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2590..3879
                     /locus_tag="O2458_000678"
     CDS             2590..3879
                     /locus_tag="O2458_000678"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ3886562.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4172..5266
                     /locus_tag="O2458_000679"
     CDS             4172..5266
                     /locus_tag="O2458_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ3886563.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5356..5448)
                     /locus_tag="O2458_000680"
     CDS             complement(5356..5448)
                     /locus_tag="O2458_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886564.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5457..7193)
                     /locus_tag="O2458_000681"
     CDS             complement(5457..7193)
                     /locus_tag="O2458_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCZ3886565.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7484..8026)
                     /locus_tag="O2458_000682"
     CDS             complement(7484..8026)
                     /locus_tag="O2458_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ3886566.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8328..9365)
                     /gene="mutY"
                     /locus_tag="O2458_000683"
     CDS             complement(8328..9365)
                     /gene="mutY"
                     /locus_tag="O2458_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCZ3886567.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9517..10494
                     /locus_tag="O2458_000684"
     CDS             9517..10494
                     /locus_tag="O2458_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ3886568.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10755..11591)
                     /locus_tag="O2458_000685"
     CDS             complement(10755..11591)
                     /locus_tag="O2458_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886569.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11600..13117)
                     /locus_tag="O2458_000686"
     CDS             complement(11600..13117)
                     /locus_tag="O2458_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886570.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(13132..13446)
                     /locus_tag="O2458_000687"
     CDS             complement(13132..13446)
                     /locus_tag="O2458_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCZ3886571.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13424..14242)
                     /gene="recX"
                     /locus_tag="O2458_000688"
     CDS             complement(13424..14242)
                     /gene="recX"
                     /locus_tag="O2458_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCZ3886572.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14503..15312)
                     /gene="sgtB"
                     /locus_tag="O2458_000689"
     CDS             complement(14503..15312)
                     /gene="sgtB"
                     /locus_tag="O2458_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCZ3886573.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(15654..16169)
                     /locus_tag="O2458_000690"
     CDS             complement(15654..16169)
                     /locus_tag="O2458_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ3886574.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(16300..16461)
                     /locus_tag="O2458_000691"
     CDS             complement(16300..16461)
                     /locus_tag="O2458_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886575.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            16788..16889
                     /locus_tag="O2458_000692"
     CDS             16788..16889
                     /locus_tag="O2458_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886576.1"
                     /translation="MRFFAFFIFLIFSVLQQNGIKNGIICSYFSFTY"
     gene            complement(17215..17400)
                     /locus_tag="O2458_000693"
     CDS             complement(17215..17400)
                     /locus_tag="O2458_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886577.1"
                     /translation="MSDYKLKIIELIKSDITGYQIHKQTGVAQYVISQLRQGKREVDN
                     LTLNTTEKLYSYARQVL"
     gene            complement(17402..17512)
                     /locus_tag="O2458_000694"
     CDS             complement(17402..17512)
                     /locus_tag="O2458_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886578.1"
                     /translation="MTEQMYLILFLLSLPLLLFIGRKTHFYCLDKKNGRR"
     gene            complement(17583..17735)
                     /locus_tag="O2458_000695"
     CDS             complement(17583..17735)
                     /locus_tag="O2458_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886579.1"
                     /translation="MCKEVKSLMLDIIKTLLEHQVLAVLIIPEVLKQLREWHLGYLDR
                     KPNNKD"
     gene            complement(17978..19423)
                     /locus_tag="O2458_000696"
     CDS             complement(17978..19423)
                     /locus_tag="O2458_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCZ3886580.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQYNADGWYGFQCFDYANAGWQVLF
                     GYNLKGVGAKDIPSANDFNGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGVQQPGSGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQASKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKFIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYGYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            complement(19404..19841)
                     /locus_tag="O2458_000697"
     CDS             complement(19404..19841)
                     /locus_tag="O2458_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ3886581.1"
                     /translation="MDINWKLRFKNKAVLTSLVGALLLFIKQVTDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSDKEEQQVTWKSSQDSSLT
                     PELSTKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKTN"
     gene            complement(19897..20292)
                     /locus_tag="O2458_000698"
     CDS             complement(19897..20292)
                     /locus_tag="O2458_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886582.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSYFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            complement(20298..21470)
                     /locus_tag="O2458_000699"
     CDS             complement(20298..21470)
                     /locus_tag="O2458_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCZ3886583.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFITYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLNKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVKKIQDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSAEDFEEFKQTLNDLYAPKNHNH
                     DERYVLSSQAFTKQQADNLYQLKSASQPTVKIWTGTENEYNYIYQKDPNTLYLIKG"
     gene            complement(21483..23381)
                     /locus_tag="O2458_000700"
     CDS             complement(21483..23381)
                     /locus_tag="O2458_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCZ3886584.1"
                     /translation="MGLPNPKTRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVENLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVAPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTGSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSSLNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKKIGLKGI
                     YFIRDKYK"
     gene            complement(23518..23817)
                     /locus_tag="O2458_000701"
     CDS             complement(23518..23817)
                     /locus_tag="O2458_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ3886585.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDK
                     LDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWILGLVGTIFSTIVIALLRTIFGI"
     gene            complement(23858..24031)
                     /locus_tag="O2458_000702"
     CDS             complement(23858..24031)
                     /locus_tag="O2458_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCZ3886586.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPETKDEKSQV"
     gene            complement(24035..24412)
                     /locus_tag="O2458_000703"
     CDS             complement(24035..24412)
                     /locus_tag="O2458_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCZ3886587.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASKQQSESDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            complement(24412..26235)
                     /locus_tag="O2458_000704"
     CDS             complement(24412..26235)
                     /locus_tag="O2458_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCZ3886588.1"
                     /translation="MVVDNFSKDDNLIELKTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTVVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQHGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFNK
                     IVEKEQTIFARVNEVEQQINGADLVKGNSTVNWQKSKITDDYGKAIESSEQSIDSVLS
                     TVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNALNQAKQYVD
                     GKFQSTSWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWAAPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            complement(26235..28145)
                     /locus_tag="O2458_000705"
     CDS             complement(26235..28145)
                     /locus_tag="O2458_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886589.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDTFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWIDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENL
                     MIFRREYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDVKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGN
                     SGWSIIEGKEVE"
     gene            complement(28160..30061)
                     /locus_tag="O2458_000706"
     CDS             complement(28160..30061)
                     /locus_tag="O2458_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ3886590.1"
                     /translation="MTITVKPPKGNGAPIPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            complement(30070..31017)
                     /locus_tag="O2458_000707"
     CDS             complement(30070..31017)
                     /locus_tag="O2458_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ3886591.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFSGVINPKQKGNKVAFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRQYTFENVSKDTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDKIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            complement(31030..34497)
                     /locus_tag="O2458_000708"
     CDS             complement(31030..34497)
                     /locus_tag="O2458_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886592.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ADDKKLQKTIKVAKKSLDAFSNKKVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPE
                     IKLQKQKLTKDIAEAEAKLSELEKKRVNIDINADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAENSSVASRFFNMMNTTGVSVFNKLLSAAGDFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKTVASVVWNAILKAITTAVSNVYNFIMIIWNQIVAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVVQG
                     FVNVVNKVSQGMTNAVNKVKSFVDDFVSAGADMIRGLMRGIGNMARDLAEKAASVAKG
                     ALNAAKRALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKIEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            complement(34514..34858)
                     /locus_tag="O2458_000709"
     CDS             complement(34514..34858)
                     /locus_tag="O2458_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886593.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            complement(34888..35253)
                     /locus_tag="O2458_000710"
     CDS             complement(34888..35253)
                     /locus_tag="O2458_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCZ3886594.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVETEEKPAEA"
     gene            complement(35315..35896)
                     /locus_tag="O2458_000711"
     CDS             complement(35315..35896)
                     /locus_tag="O2458_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HCZ3886595.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDSSTVTPGEV"
     gene            complement(35915..36298)
                     /locus_tag="O2458_000712"
     CDS             complement(35915..36298)
                     /locus_tag="O2458_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886596.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     IAQLVDDTTNQELLHTSITISYKTF"
     gene            complement(36310..36657)
                     /locus_tag="O2458_000713"
     CDS             complement(36310..36657)
                     /locus_tag="O2458_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCZ3886597.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            complement(36657..36959)
                     /locus_tag="O2458_000714"
     CDS             complement(36657..36959)
                     /locus_tag="O2458_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886598.1"
                     /translation="MRYEDRVIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            complement(36956..37288)
                     /locus_tag="O2458_000715"
     CDS             complement(36956..37288)
                     /locus_tag="O2458_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCZ3886599.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            complement(37297..37584)
                     /locus_tag="O2458_000716"
     CDS             complement(37297..37584)
                     /locus_tag="O2458_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886600.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYNPTDERIKALA
                     SVENKRNKQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            complement(37606..38580)
                     /locus_tag="O2458_000717"
     CDS             complement(37606..38580)
                     /locus_tag="O2458_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ3886601.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADKRTDSVPGEV"
     gene            complement(38594..39214)
                     /locus_tag="O2458_000718"
     CDS             complement(38594..39214)
                     /locus_tag="O2458_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCZ3886602.1"
                     /translation="MEENKLKFNLQFFADQSDDPDEPGGDGKKGNPDKKENDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAKEKAAEAAKEAAKLAKMNKDQKDEYEREQM
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            complement(39323..39493)
                     /locus_tag="O2458_000719"
     CDS             complement(39323..39493)
                     /locus_tag="O2458_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886603.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKRNETVIIDANLDSLRSAVLA
                     DKEKSKYNEPLF"
     gene            complement(39566..40561)
                     /locus_tag="O2458_000720"
     CDS             complement(39566..40561)
                     /locus_tag="O2458_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCZ3886604.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKEFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPSKKRKTKYFGGKVKSGD"
     gene            complement(40568..42103)
                     /locus_tag="O2458_000721"
     CDS             complement(40568..42103)
                     /locus_tag="O2458_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ3886605.1"
                     /translation="MLKVNEFETDTDLRGNINYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIAGVRYLRTKPIDKTDEDGVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENGFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTWSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            complement(42114..43391)
                     /locus_tag="O2458_000722"
     CDS             complement(42114..43391)
                     /locus_tag="O2458_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCZ3886606.1"
                     /translation="MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNLSSKVDTIKSLGL"
     gene            complement(43378..43818)
                     /locus_tag="O2458_000723"
     CDS             complement(43378..43818)
                     /locus_tag="O2458_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCZ3886607.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES"
     gene            complement(44005..44427)
                     /locus_tag="O2458_000724"
     CDS             complement(44005..44427)
                     /locus_tag="O2458_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RinA family phage transcriptional activator"
                     /protein_id="HCZ3886608.1"
                     /translation="MTKKKYGLKLSTVRKLEDELCDYPNYHKQLEDLRSEIMTPWIPT
                     DTNIGGEFVPSNTSKTEMAVTNYLCSIRRGKILEFKSAIERIINTSSRKEREFIQEYY
                     FNKKELVKVCVDIHISDRTAHRIKRKIISRLAEELGED"
     gene            complement(44451..44597)
                     /locus_tag="O2458_000725"
     CDS             complement(44451..44597)
                     /locus_tag="O2458_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886609.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFED"
     gene            complement(44598..44771)
                     /locus_tag="O2458_000726"
     CDS             complement(44598..44771)
                     /locus_tag="O2458_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HCZ3886610.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEKVYIMTTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            complement(44771..45157)
                     /locus_tag="O2458_000727"
     CDS             complement(44771..45157)
                     /locus_tag="O2458_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886611.1"
                     /translation="MMIKKLKNMDWFDIFIVGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     EVKTIGYRIHFLNLYPVLYEVKKVDK"
     gene            complement(45147..45383)
                     /locus_tag="O2458_000728"
     CDS             complement(45147..45383)
                     /locus_tag="O2458_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886612.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            complement(45408..45644)
                     /locus_tag="O2458_000729"
     CDS             complement(45408..45644)
                     /locus_tag="O2458_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ3886613.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            complement(45641..45886)
                     /locus_tag="O2458_000730"
     CDS             complement(45641..45886)
                     /locus_tag="O2458_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886614.1"
                     /translation="MSYIITLVLILVFIVIFNNLLNRYMVLYKELDLFTCRIGMLLVL
                     IVIVDFAKQQNMLATLSVLLILLFVEKLRIIQRSDKK"
     gene            complement(45923..>46321)
                     /locus_tag="O2458_000731"
     CDS             complement(45923..>46321)
                     /locus_tag="O2458_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCZ3886615.1"
                     /translation="EPQEKAVIKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKID
                     AGYHGNLGINIKNDAIASNGYITPGVFDIKGEIDLSDAIRQYGTYQINEGDKLAQLVI
                     VPIWTPELKQVEEFESVSERGEKGFGSSGV"
BASE COUNT        13672 a         8836 c         6638 g        17175 t
ORIGIN      
        1 taaatttcct aactcaatta attcaacaaa tattatttta ttatgtattt gttcgaattt
       61 caatagtcaa atttacactt tattaatatt ttaaatcaac aacttaaaac atttgttttt
      121 taaatattag tgccgtttga cgacttttat ataatatcaa gttattctag tagagtcaac
      181 ataaaacaac acttattttt gtttaaaaat tactgttgaa gggattattt tctattcgta
      241 agtactatat gtatttaaag tgtaattcct tatgcttttt acttcagttt tacaaacaaa
      301 cttatactca ctttatggat agttatttag tagaccttcg tttgaatatt tttatgttta
      361 tttataaagg atactagtta ttagttaata ccacttccgt agttagaata ttcttttctt
      421 caatttaaat atttttcaga ataagcataa aaaaaagtta aacaactttc atgtcgttta
      481 acttcttaaa tattacgctt acttatatga agtaagctaa cgctttttta atttgtcata
      541 ctaccaaagt taaattattt atcttggcat tctttacaaa ctccataaat ttccattcga
      601 tgatgtgtta cgtcaaagtc agtcatatgc tgagctaatc tttcaatttc atttaactgt
      661 ggatattgaa aatcaacaat cttaccacat tgttcacata taatatgata atgattatgt
      721 gtattaaagt cgaatcgact tgatgagtct ccatatgtta attcttttac aattccaata
      781 tctttaaaca ctcttaagtt attatatatt gtcgcaacac ttatatttgg aaaatcaggt
      841 gaaagtgctt gataaatttc atcagctgtt ggatgagtat gtgaagaaat taaataacgt
      901 aatattgctt gtctttgagg tgtaattctt acgcctgctt gtcgcaatga tgcaattgat
      961 tcttctagtt catgttcaat tgattctatt tcaacactca tctatatcac catctttctt
     1021 atataataat tattattact tactataaat ataactgtag tattgcgcgc gtgtcaatat
     1081 ttacatgatg atttcattta atagcctttt ttagcatcaa cttcattctc aattagacca
     1141 ttcttattga gaaaattaac tagattgttt ttaaaaatat ctaataagtc atactttgct
     1201 tcataatcat taccagttat atgcgctgtt atagttacat tatccaattc atataattca
     1261 tgattaggtt tcaaaggttc attttcaaac acatctaaat atgcatgtcg aataacttta
     1321 ctttttaata cttctattaa gagcgcttct ttaactatgc tacctcgtcc tatatttata
     1381 aaaagtgctt catctttcat taattcaaaa tgttttttct ttagtaaatg aatcgtttct
     1441 tgcgtttctg gtaaagcatt tataataatg tcagcatttg gtaatgtgct ttcaagtgat
     1501 tcgatagtat atatctcgtc aaactcatct ttgttctgac ctgacttgct cagaccaatt
     1561 aaattcatat taaaagcctt tgctaacttc gcagttctag tagcaattgc acctgtacct
     1621 aaaaataaaa ctgtttgtcc tgataggcgt ttaccagtta ttttcgaatc atatatatgt
     1681 tgtcgttggt tatcatatga tagtttcatc tttttataat catctaaaat gaaagctaaa
     1741 atgtattcag ataattgttt agcttgaaca ccttttccat tagttaaaag tatgccgtga
     1801 tctgcaatat aatctaacgg caatgtattt acacccgttg caaaccatgc aatccatttt
     1861 aaattcgggc aacgtcgtaa aaatgcctca ttgataccgc catcataacc aactaatata
     1921 tctaaatcag ccaagtcgct ctcaggtatt tctgatgctt ttttataaaa tttaaaatct
     1981 aaatctgaaa agcgttgttg taattcagtt tcaacttcac gcatacgatt taacccaaca
     2041 actttcatat atgtcacccc aaaatgtttt taagttcttc tatttgtgtt ttaaccttaa
     2101 ccttctcgat aacatctaat actttacctt gttcatctat tataaaagtc gttcttacaa
     2161 tgcccatact ttctttgcca aatgattttt ttaactgata tacgccagtt tctttagcta
     2221 atttaaaatc ttcatctact aataaatcga aattcaatcc gtgtttctca ataaaatttt
     2281 ggtgtttttt ctttgaatca ccgcttatac catatactgc aacatctaaa tcattgaaca
     2341 tttctaaatt gtctctaaag tcacaagctt ctgtggtaca agtaggtgta ttatctctag
     2401 gataaaaata tataatcgcc tttttacctt ttaatgtatc atttgtaatg acagttccgt
     2461 cttgattttc taatttaaat attggaaatt gttctccttt ttgcaacata taatcacctt
     2521 tttcttcatt ataataattt tatgatacga tattagatga aaatattaaa ttaaaagagg
     2581 ttgatataaa tgaattttag tgaaagtgaa cgtttacaac aactttcaaa cgaatatatt
     2641 ctaggcggtg tcaattcccc ttctcgttct tataaagctg taggaggcgg tgcacctgtt
     2701 gttatgaaag aaggacacgg tgcatattta tatgatgtcg atggcaataa atttattgat
     2761 taccttcaag catacggtcc aattattacg gggcatgcac atcctcatat tactaaagca
     2821 attcaagaac aagctgctaa aggtgtttta tttggtacac cgactgaatt agaaattgaa
     2881 ttcagcaaaa aattacgtga tgcaattcca tctcttgaga aaattcgctt tgtaaattct
     2941 ggaacagaag cagtcatgac aacaattcgt gttgcacgtg catatactaa aagaaataaa
     3001 attataaaat ttgctggatc ttatcatggc cattctgatt tagtattggt tgcagcaggt
     3061 agcggcccat ctcagctcgg ttctccagac tcagctggtg ttccagaaag cgtcgcacgt
     3121 gaagtcatta ctgtaccttt caatgatatt aacgcctata aagaagcaat tgaattttgg
     3181 ggtgatgaaa ttgccgcagt attagtagaa ccaattgttg gtaactttgg aatggtaatg
     3241 cctcaacctg gatttttaga agaggttaat gaaatttcac ataacaatgg gaccctagtg
     3301 atttatgatg aagtaattac tgcattccgt ttccattacg gtgccgctca agatttatta
     3361 ggtgttatcc ctgatttaac tgcatttggt aaaattgttg gcggtggttt accaattgga
     3421 ggctatggtg gacgtcaaga tattatggaa caagtagcac ctctaggacc tgcatatcaa
     3481 gctggtacaa tggctggtaa cccgttatct atgaaagcag gtattgcatt actcgaagta
     3541 ctagaacaag acggtgttta tgaaaaatta gacagcttag gccaacaact agaagaaggt
     3601 ttacttaaat taatcgaaaa acataatatc acagctacaa ttaatcgtat ttatggatct
     3661 ttaacattgt actttacaga tgaaaaagtc acacattatg atcaagttga acattctgac
     3721 ggcgaagcgt tcggtaaatt tttcaaatta atgttaaatc aaggtatcaa tttagcacct
     3781 tctaagtttg aagcttggtt cttaacaact gaacatacag aagaagatat taaacaaact
     3841 ttaaaagctg cagactatgc ttttagtcaa atgaaataac ttttaaggaa gtatattttt
     3901 aattgaatta aaataaagca aatgctcaca tagtagattc attttatatc gttaactatt
     3961 aacgtttaac ttgatatgtc actatgtgag tttttatttt acaaatttat actattttaa
     4021 tctctaacta aagttttaca tcgctatcag tattatgtat gatttatttt aacaactata
     4081 aataagcttg aattttgtaa ctagtaagtg tataacagta atgaatacga atattagata
     4141 ttcaataaac ttaaaagggg cgtcattatt tttgagacta ggagctcgga tttttaaaac
     4201 tggtatagcc attattttag ctatgtctat cgcttcttta ctaccggatg atgttggtct
     4261 gaaagcctta gctggtgtca gtgctgttgt tgcaatgcaa cctagtattt atcgttcatt
     4321 taaaacagtt tctgatcaag cattgggtaa cattataggt gccatactat ctgttacaat
     4381 ggtaacaatt tttagtgata attttatcat tatgggtgtt accgtcatag tacttattgc
     4441 tattttattt aaatttaatt tagcacatgt tgctactttg gcgagtgtaa cagcattaat
     4501 cattatggga caacataccg gttctttcta tattaccgcc ttttacaggt ttgtacttgt
     4561 aatgattggt gtcataagtt catctctagt taactttgtg tttctaccac ctaaatttga
     4621 aacaaaaatt tactataatt cattaaatat atcctcagat atttttatgt ggtttaaatt
     4681 agtattaaat gatacgacgg agtttaataa tattaaacaa gatagtcata atttaaaaca
     4741 acgtgtcgaa aagttagaga aaatttatga ttactatagc gaagaacgtc ctattacaaa
     4801 aaaacatatt caccaacaga atagaaagaa aatacttttc agagaagtag ttcagacgac
     4861 tagacaagct tatgaagtat taaacaaatt gtcgcgttat caaaacgact tatatttact
     4921 taataacaat ttcctattac aaatcaaatt agatttagat tcattaactg cttttcacga
     4981 gcaaatttta gctagccttt ctaaaaaagc acgttataat gtgacacatg ttgattatga
     5041 gttagataat ccgcagaaaa aagatttatt gtccacattt cagcatgagt taataaatca
     5101 tccttatcaa acggaatatt cgtttgccaa tgttatgcaa attgttgcag ccatcgaaga
     5161 gtatcgacac catttagagc atttagaccg cataagaatt agctttttca catatcatag
     5221 atctgatgca gacattgaaa ttgttgaaga agactttgat ttataacatt aaaacaattt
     5281 tatcaattaa tgccacaatg agataaacta atattgaatc tcgaaattaa atttaaaaaa
     5341 gcagtaagat tattttcaat taagaaaatc tcttactgct ttttctatgt tatgacccca
     5401 ccatcctgtt acaaattttc tcttcaagat tgtactgaag tcaaacaact aatactttat
     5461 aagttttgaa tgctatataa atgctcgtaa gcaccttgtt ttgcaatcaa ttcacgatgc
     5521 gtacctgttt caacaatatg tccattttca attacgacaa ttttgtcagc atgtgtaata
     5581 gtggacaagc gatgcgctac gataagtgtc gttcgatctt tactcaacac atctaatgct
     5641 tcttgaataa tggattcact ttctaaatca agtgcacttg ttgcttcatc caagataaga
     5701 attggcggat tatttaaaaa tattctagca atcgataatc tttgtttttg accacctgat
     5761 aatttaacac ctcgttcacc tacttcagtg tcatatccct gtggcaagtt cataataaag
     5821 tcatgtgcat tagccatttt cgccgcttca actacttctt catctgttgc tgttggacga
     5881 ccaagtaaaa tattttcctt aactgtatca gagaataaaa tattatcctg ttgcaccaat
     5941 cctatttgat ttcttaaact tcccgttaaa aaatctttaa tgttgtgacc atctattaaa
     6001 atttgcccag aagttacatc gtaaaatctc ggtattaagt taattaatgt tgatttacca
     6061 ccaccactca tacctacgaa agcaactgtt tctccttttt caatactcaa attaatatct
     6121 tttaaaattg gagcttcgtt atcgttatat tgaaaactaa catgatcaat atcaatacga
     6181 ccttgtttaa tttcaatagg ttgagcacca acaccatttt tgatgtcata atcttcatca
     6241 attaattgga atacacggtc cattgaagca aaactttgcg ttaaagttgt aaatgatgcg
     6301 actaaacgac gtaaaggccc gaacaataac tctaagtatc caacaaatgc tgcaagtgta
     6361 cctactgtga ttgatccaga aatagcaaga tatgcaccaa caccgatgac aataattggt
     6421 ccaatatctg taactgtatt aattgcggca aaggaatagg cattccatct tgtatgtttc
     6481 aacgcacgtg ttaggaaatt agtattcttt ttatcaaagt ttttcgcttc attgtcttca
     6541 atcgcaaaac ttttaacgac tgaaatacct tgaacacgtt catgcaagaa tccttgaacc
     6601 tcagctaatg cttgagatct ttcacgtgtc aattttctta atcttccaaa gaaaacgtac
     6661 accgttaaaa tgtaaaatgg gaagataaac agtgctgcta aagtcaattt cacatctaaa
     6721 aagaacatta tggatagtgc aataataatt gttatacaat ctaaccaaat attcattaac
     6781 ccggttaaaa tgaaatcttt tgtttgttca acatcattaa tcactctaga tattacttga
     6841 cctacttgat tattagcata aaatctcgca cttaaagctt gtaaatggtt gtataacttt
     6901 ttacgtatat catacaatat tttattactt gtccattgcg ccaaatattg acgtataaat
     6961 tcaattggtg gtctcactat tacaaaaata aataatgcga taccaatggc aatagttaaa
     7021 tgatgaactt tttcatcagt cgttagtgcg tggttattaa tcacgccatc tattgcatat
     7081 ttaattaata gtggtataag cattggtata ccaaacttaa ttatcccaac aataatcgtt
     7141 gcaaaaatac gatatttgta tggcttaaca aattgcaaat atcgtttaat catacaatcc
     7201 cccctaatct attgccctat cctattcata agcataaaaa tgaatagagg ttggatacat
     7261 aatttgtaga tgtaaattct tcttacaatt tacattttta aaattaagat atctcaacct
     7321 ctgtcatatt catgtgatta aatacgttgt cttattcgta tgttatattc tattcaattt
     7381 aatctatgga tactgtgtcc ccacacgaca gcaaaagtta tcatacttct ttacatcact
     7441 aagtcaatat aaatgattta atcagtattt acactttatt tgcttaatac tgtctaattt
     7501 ttttgtaacg ttctttccaa actttgataa aatctggcgc gaatgggccc ttcttctgtt
     7561 ctatccattg ttgaagaatg tccacgttgc gtcttaaaat aatatcaata tcatgcggat
     7621 aattcatttg attcatatgt tgctcatatt catcttcatc taataaatga tactttccgt
     7681 ttggatatac tttaatatct aaatcatagt ctatatattt taatgcctct tcatcacaaa
     7741 caaatggtga tgacaaattg caatagtaat aaattccatc ttctctaaac atgcagataa
     7801 cattaaacca atattctgag tgaaagtaaa caattgccgg ttcacgtgtt atccaagttc
     7861 ttccgtcact ttcagtcact aacgtatgat catttccacc aatgacaaca tgatcagtac
     7921 cctttaatat tgttgtttca gaccaaacgc gatgaatctt accatcatgt ttataactct
     7981 gaattttaat gttttcccct tctttaggta tggattctct gaccatactc cacaccacct
     8041 tctgttaatt taaccattat aaattatagc atatttcaga aatagtatta tataaataca
     8101 tatttttacg aaataagatt ttactactta ataattaaac tcggtaatat tgctaagtac
     8161 tacaacagag atttacatgt cccatttaaa gtatataaaa tcatcacttt tatatatcaa
     8221 cactttaact ttttgacatt gttattctat gagatttaaa gatatcattt atacttttta
     8281 aaattaatgt cactatgttt tccgataata ttaccaatca tcgaatgtta cccatttata
     8341 aattgataaa tctttgacat aggtacaggg aatgtatatt gatctcgatc acttaaatca
     8401 aaccaaatca tgtcatctgg taatgtttca atgttaattg ctcctgaaac ggcgtatact
     8461 ttaatcttcc atgttaaatg agtaaattga tgcttcaact caaaaatagg tgtttctact
     8521 ggttgaatgt catgaccgat tttttcagtc attttacgtc tagcatgctc actttcaaac
     8581 ataggaaatt gccacatacc atgcaataat ttttcgctac gcttttgcaa cagatattga
     8641 ccttgattat ttctaattaa aaagacggat tgctcaatta cttttttact tacattttta
     8701 gatttaacag gtaacttttc aaatgtacct ttatcaaatg cctcacagtt ttcttgaact
     8761 ggacaaaata agcataatgg attttttggt gtacaaatta acgcccctaa ttccatcata
     8821 gcttgattaa acgttccagc ttctgtagta acatacggta ataattcttg ttcgtacgat
     8881 ttcctcgtcg attgtaattt aatatctcga tagtcatcat tcaatctaga ccatactcga
     8941 aaaacatttc cgtctacagt tgctagtggt acattatatg caatgctcat tactgcagct
     9001 tgtgtgtatg ggccaacacc ttttaacgct ttaaattgat caggatcttt gggaactaag
     9061 ccttcatatt tatcatgaac ttctttaatc gccgtatgaa aatttcgagc tctactataa
     9121 tatcctaagc cttcccaata ctttaacact tcatcttccg aagcttgact caaaacttcc
     9181 acagttggaa atcgttcaac aaaacgatga taatagtcaa taactgtttt aacttgtgtc
     9241 tgttgtaaca tgacctcact taaccaaata tagtacggat tggtcgtttg tcgccatggc
     9301 atttctcttt gattttcatc aaaccagtgt atcaaatttt ctttaaaact agactgctga
     9361 tacatttata aaaccctttc ctcaccaaaa ttaattgtct ttactcataa tgtttttatt
     9421 gtacattaaa atcatggtta gtatgtaagt taatttagtt atttgcgaaa ttggattata
     9481 atagtatata taatattatg aaatgagtga actgatatgg acactgcaac acatatcgca
     9541 attggggtgg gccttacagc acttgcaact caagatccag caatggcttc tacgtttggt
     9601 gcaacagcta caacccttat cgttggttca ttaattcctg atggggatac tgttcttaaa
     9661 ttaaaggaca atgcaacata tatttcgcat catagaggta tcacgcattc catccctttc
     9721 acaatactat ggccaatttt aattacattt ttaatattca cgttctttag tggaaccaac
     9781 ccatttcatg tatggatgtg ggctcagctc gcagtatttt tacatgtctt tgtagatata
     9841 ttcaattctt atggtacaca agcgcttaga cctatcacaa acaaatggat tcaattaagt
     9901 gtgattaaca catttgaccc tattattttc acagttcttt gtattggtat tgtattatgg
     9961 gttataggct tgcatccatt tgcagtcttc tttcctataa tcgctttact aatcatttat
    10021 tacatgattc gttttaaaat gagagccgta attaagcaac aagctttaaa agcaattcaa
    10081 caagagcatc accctgttaa agtatttgtt gcgccaacaa taaaatttat ggaatggcgt
    10141 gtcgcgatac aaactgatgc acatgactat gttggaaaag catatggtag gaatgtggtg
    10201 tttagtgata aagtggaacg tcaaacatta tcaacagact ccattttatg gaaagtcaaa
    10261 ggtaataaag atatacgtac ttttttaaac ttttcatcaa tctatcgttg gcaaacaaca
    10321 acgttagcag atggttctac tgaaattcgt ttgattgatt tgcgttattt aaaaaatgat
    10381 cattattcat ttgtggcaat tgcacatgta acaaacgata atgtcataga ccactcttat
    10441 attggctggg tatttacaga agataagtta caacgtaaac tgtatgctaa ataatttcaa
    10501 gttattattc actaaagtta atctataaaa aatgaacaac cgggcagaat gaaaatcaaa
    10561 acgattttta ctctgtccgg ttttttaatg taaaactatg aatgctttta caaaatctaa
    10621 aatttatatt gttgctaaca aactaccttt aatgactcga aatatcaaaa tcagtatagg
    10681 aaaacaatat ctagatgata ttctaattgt ttctgattct cacagattaa tttacacaac
    10741 aggtcagcta aacatcatga agaagtatcc gcctcgtctg tactatcatt tgaaacatcc
    10801 tgttgattat cagtttgtgt cactttagat gttttattat aaattgcgtg tgtcgtatac
    10861 cttgctaata cgaaatttac tgcagctatt aaacataaga ataaaataaa gtaaaaatga
    10921 tatggaatgt tttccatact tgatatacca aatatgatag attgtgctat accattaaca
    10981 atgtaataca ttggatttag cattaggatg tgattgataa atacatgatt tggatttggt
    11041 atgaaaataa ttggtaacaa taagaaacac aatacacaaa ccccataaaa tatgatattt
    11101 attttttcag ttaacagtcg aataagacca aaagtaacgg atattaatcc tacaaaaata
    11161 gttgccatca caataaaata gaatagcgct atatatgatg tttcgaagtt tactggttta
    11221 accaatgcac taatcatcgt caaaatgact agcataataa aacttaaaat agacataata
    11281 actactggcg tcgctgcgga aatattaaat aacttagtaa ctaaaaagtc attcatataa
    11341 tgatgataag agatataaat tgtgaacatc actaccgcaa aaacaatcaa tacaatgatt
    11401 ctaaataacc atctcacctc tttaacatcg gtcaaatgac taaaggaaaa tagcgtttca
    11461 cttgcaataa taagtattaa acatgcagct aatacgatta gaaaactttt ccaactttgt
    11521 tttaaacgtt ctgttacata ccttttagta tgcggtaagt ttttaaaata taaaataata
    11581 ttatctagca tctttacacc tacaattcaa tataaatcca tttgtcttct ttcaaattgg
    11641 caatacaata tccattccca actttagtaa tccaaatgtt attgttaata ttaaacttat
    11701 cttttaattc ttttttatct acaattggat aaacaaaacc gtataactga ttattatcat
    11761 tgaaaagtaa ttgtatcggt tgttgtgtaa cggatgcaac gaaacggtta tcatcatcag
    11821 gaactaaagt ttttataact gaactatgct ttttatgtaa ttgactatta aagtatgcat
    11881 agtaattact ataatttgat ttcatatatg gtgctaattt tttaaaacta tgcgtttgat
    11941 ataaacctgc cgggttaaaa tattctaatt tatctttact aacatagtaa gtcttattat
    12001 cggcggtaac acgataattt ttactatttt cgccagtaat tgtaactaca ctatattttg
    12061 gaattgtgac ttgtttatcc ccttgcatat ccacactgcc attaaaagca attccataag
    12121 ctaacttttc gccatatgga tctttatttt cattttgcat cgtagcacga ttcgtaaaat
    12181 ctgttatcga aataattcct atattattga aaatgagcaa catcatcaat gctaagccta
    12241 aaatagtacc tgaggccaaa gtccaaaaac gcactaaaaa cttaggcggt tttgcatgat
    12301 tatagcgctc aacacgtttg aaattatagg tcatctctgg tattcttgta cgattctttt
    12361 tccaatctaa atcaaagttt tcaatttctt cttttgagtt tagacttaat cgatcacgtt
    12421 catgttcttt aaaagatgga ataacttgtt taagtgaccc ttccattctt aattgaccat
    12481 gtgaaaacca agctatgtag ttactcactt gtgaaatttt atcaatatca tcaccaattg
    12541 acacaatcgt taaattattt tcaatataat catttgttaa ttcaatcgca cgttccataa
    12601 attgtggtgt taaatagtca ataacatgat ttaaaataat aatatttgat tttgatgagc
    12661 gtgcaatact taatagtaat tgagcgtatg ccgctttcga aatatggtta accggcttcg
    12721 ttttataatc acctaaatgt gcatattgaa taatctgttc agctttatga tcattaattt
    12781 catatggaaa tagttgaact aactgcgctg tataagcttc aacagtttga tgaattaacg
    12841 attgatcttc aatatatccg tagaacaaat cttcagtaca aactacttta cctttatcag
    12901 gtttaattgc acctgccaac aattgaccta ccaacgcttt ggaagattca ggttcaccaa
    12961 ttatacctaa tgcttctcct tgataaatat gtaaactaat attgtttaaa tcgatatctt
    13021 cagcatcata tccaaaaggt aaataccatt tcttattctg tttattccta tagtagtgtg
    13081 ttacttttag taactttaaa acaattgaac ttcccatcta ttttcatcct tctataattg
    13141 taataacgct ataggtaatc cttcttcggg ttcatcgcta ttaaatctaa acccccacgc
    13201 aaaaatacct tttaatcttt ctactttaaa atattgatta ctgccatcag tcagtttata
    13261 aattttacca atttctatct tttttctgtc tactaagtaa ctttcggcaa caattacttt
    13321 actttgataa acgtcatatt catttaaaat accattcatc tcagcttttc tcattttctc
    13381 tttataaagt tgtatttcgt gtcttaattc tggctcagac atctcactca atttcttctg
    13441 ttccatcggc aataccactt tcttctaatt tagctttaat tttatcatat ttatatcctt
    13501 ttctcataag gccttcgata gtttttgaaa ttaatttctg ttgcgtgtac ttctttcgat
    13561 ttttattata aattttttct aaatctcgtt gtaataaatc gtctaaaacc gcttcatctt
    13621 gtgtaaaatc catttcattc aatacagcat gaatggtttc catttcaaac cctttttgaa
    13681 ttaaagattg cattactttt tctttaactt tattttgtgg cccttttttt gttttagata
    13741 ttttttcagc aatttggata atatcatcaa gttcctgttg ttctctataa agttctgtaa
    13801 atatttcaat gatatttggt tctataccaa gttgatatag tttttgttga taaattttag
    13861 gtcctttatc tgtcgtgcga atcattgtat tttttaaact ttccgcataa tcttgatggt
    13921 cgattaactt ttcgcgataa caatattcaa tcacttcaga aatcgcttgc tctgatatct
    13981 cttctttttg taaatattgt ataacttctt tttcagttct ctttttatat gataaatatt
    14041 ggattgcttt atttaaacct atgcgataat gatcatactt ttgaatctct gccatgtcag
    14101 cagcttcaag ttgttgccct ttttttaaat taaatttgac taatgtatca atatctatac
    14161 ccatttcaaa ttgttcgtct aaaaaaagat taaaccgttc tttgtttttc ttttgaactt
    14221 ctattttagt aatcttcggc actggatcac cccgttactt tcttaagttt taatacaata
    14281 ctcttttatt attcaataag ccacttccta tagcaaatgt ttaactttaa atatttttcg
    14341 atgctaacaa aaaatcacac tatcatcttt taaaatgaaa gtgtgattac aagcaaatct
    14401 gtaaaattta taaagcagaa acaattcaac tttatcatta tgacatttca attaaacctt
    14461 ctacattata gttccaagca tcttacacat gaatgcaagt atttaacgat ttaattgtga
    14521 catagcctgt tgatattgtg tttcattgat ataattttgt tgcttcattt tttctaagtt
    14581 cgtgcttaca cgttgcgtaa aattctctga catattattg atattatata cgctaggtgc
    14641 attgacttta ctagctaaaa tagcgctttg taaaactgtt atgtgagaca ttgttgtact
    14701 atttttattc acggttgttc caaagtaatg gtttgctgcg ccctcaagcg tatattgatt
    14761 atccccaaag taaatattat ttaaataaaa gcttaaaatt tcgttcttat tatattgttt
    14821 ttcaactcga tgagctacaa ataattcttt tacttttcta gtaaatgaac gatcattatc
    14881 ataaaaataa tttttgacaa cttgttgtgt aatggtacta ccaccttgca catctctgtc
    14941 gctaatcgtt gaaaataaag ctctagttgt acctttcaaa tcgaatccat gatgattgta
    15001 gaatcgttca tcttccattg aaataaaggc acctttaaca tactctggca tgttatcagc
    15061 tgacacaaaa ctacttttat tttcaatttt tcttagttca tccacattat cgcgtgtaga
    15121 taaaaaatac atgataccaa taaacaatgc gataatgatt agaatggtta ataatatttt
    15181 taatagtatt cgtttacttt ttttcttttt cggcggtttg ccaactggtt gataatacgt
    15241 attatagtga ggttcgtgtt tcatatgctc aaaatgttca tttgagtttg agtacctatc
    15301 gcttcttttc atgcgtttgc tccttctttt aaaactcact tagtatatac cttgagttta
    15361 ccagtactat cacaaatagg ctacactttt tgggaaaatc agtccaaggg cttacaatcg
    15421 tatacgccat catacttact ttttttgttt tttgaaaaaa ttatagataa atcattgcaa
    15481 ttttaaatat taatcatgtc aaatattgtt atattttata aaaataaaag accatcccta
    15541 ttaaatgcca atagagacga ccttttattt gttattcatt tattaaaact aaaatccata
    15601 tttcatttca aacgaaaata tataaatttt aacaatcgat aaccacaata cttctattgt
    15661 aattgtttaa cgatttctcg attaaaatca tctaaatcgt ctggtactcg acttgttaca
    15721 atattgttgt ctacaactac tgactcatca actacatgtg cgcctgcatt tgataaatct
    15781 ttgcgtacat ttaatactgc tgttaacgta cgacctttta aatcgtctgt atctattagt
    15841 atttgtggcc catgacaaat ggcaaatgtt ggtacatcat ttttagtaaa gtatttagca
    15901 aatgtgccat atcgaccttc tgtatctcca cgtaaatgat ctggtgaaaa tcctccagga
    15961 attaataatg catcataatc ttctggttta gcttctgcaa tgcctacatc gacagtaact
    16021 ttttcaccgt gtttaccaac aacttcacta tttgcagtat ctccaatcac tacagtatta
    16081 aagcctgcat tctctaatgc ctctttaggg cttgaatatt ctatatcttc aaattcgttt
    16141 gctagaataa ttgctacttt tttagtcatt gcaaatcacc tttctatata tcattgatat
    16201 aattactata gacaagtaaa tcagtgatta aacatacaag atataaaaaa tattaagcga
    16261 ctgtcgcgat atctaaccct aacacatctt atgtggcatt tacttagata ctaatttaac
    16321 cttttcttca agctgatcta acaatccaat ccattcatct atatcttcaa cacgtacttc
    16381 atcaggattt acatgatcga tatcctcaat aaacttattt aaacgcgctt ttatctgttc
    16441 gattgtttgt tgttcattca taaaaagtta actcctttta ttttgttttc tttttcatta
    16501 ttatcctaac agaaattgcg ttaaagcgat ataatcttag ctatatttat gacattcaaa
    16561 ttattttgac ttttaaaaat ccccttttca attaactaaa attaagagat aatttgttac
    16621 gagtgataat acgaagtggt atcataccga tatgaaccaa atagaaagaa ggaagtttaa
    16681 gacgatgaat agcgtcaaat tgaagcaacc tgttagcatt tacaatgatc catgggaagt
    16741 gaaatttata tacatttaaa tttcatgaga caataaacgt tgatttaatg cgtttttttg
    16801 ccttttttat tttccttatt ttttctgttt tacaacaaaa tggtatcaaa aatggtatca
    16861 tttgtagtta ttttagcttc acatattaaa acaaccacac tcctaaatta ataggtggtg
    16921 tggttttgtt ggttgtgtgg ggataaaaat aaccgcatca gttaagatgc ggttatctag
    16981 caagggccac gtatttataa atacgtttag aatctcttcg gcaactttgc tatagacagt
    17041 ctatgctgtt actaaattat accaccacac aaacctactc ccattcagga acacagagct
    17101 ttgtcgctcg tcagcaacgt catatgaatt ctcagttcat gttgtggtga cactttaaac
    17161 ggtctgtgcc agtagcgacc gagtcatttc aagaatgacc atttcacatt tatattataa
    17221 cacttgtcgt gcgtaactgt atagtttttc agttgtattt aaagttaagt tatctacttc
    17281 gcgctttcct tgccttaatt gtgaaattac atattgcgct acgccagttt gtttgtgaat
    17341 ttggtaacct gttatatcac ttttgatcaa ttcaattatt tttaatttat aatcactcat
    17401 attatctacg tccattcttt ttatctaaac aataaaaatg tgtttttctc ccgataaata
    17461 ataacaatgg taggcttaat aaaaacaata ttaaatacat ttgttctgtc ataattgaaa
    17521 acctccaaat aatattatat tatataagtg taaggaggag ccatcaggct ccaagcataa
    17581 tgttaatctt tgttgtttgg ctttcggtct aggtagccga gatgccattc tctaagttgt
    17641 tttaacactt ctggaattat cagtactgcc aatacttgat gttctagaag tgtttttatt
    17701 atgtctagca tgaggctttt cacctcctta cacataattt gtaagtcatc aactaaccta
    17761 caaatataat tatactaaac aattgtttag taagcaagtg tttttttaaa tttgcataaa
    17821 aaataggcaa gtaccgtagt acctgcctgt tatctacatt taaatcttga gagaaatgtt
    17881 aaaaagttct agtaaaataa tagcacattt tatctttaaa tgtaaataga aagcaggtat
    17941 gtaacgcacc tgctcaaata gacatgacta tgtcattcta actgatttct ccccataagt
    18001 cacccaatat ctgattaggt ggggcagaac cattccatgt tctaataggc aagtaataac
    18061 gttgcccctc ccatgtatat cctacccaaa catgaccatc ttgtaacatc acttctgtat
    18121 aatcacaata tccaccaggt tggaactgat aacccactgg acaagataag aatggcccca
    18181 cttttcttac tgtgattggt tgattgccgt ttgtgaatct agcactttct tccatgtagt
    18241 aagtaccata tttattacgt ttccatgcac ttgcaactgg tttaactgta ttacttgaag
    18301 cgcttgactc attagagaca gtggcaaccg gtattttacc atccatgtac gccctaatct
    18361 gcttgataaa gtagtcttta agttgcaacc gcttgtcttc tggcaataga ccgcgagtta
    18421 ctggatcaaa accagtgtgc aatactgagc ttctgtgtgg gcatgatgtt gaagtgaatt
    18481 cgttgtgtaa tcggattgtg ttacggtttg ctggtaatcc ccattttttc aacaatctag
    18541 cacattcttg gaaagtcgcc tgttcatttt ttaaaaacgt cgcattatct gctcccattg
    18601 attgacacac ttcaataccg taaccatatt tattacctat ttgattcgct gtatgccaac
    18661 ctacttgaga ttcatctaag gcttgccaaa ctgtgttacc tgatacgtaa ctatgcgcaa
    18721 tacctgcctc tagtctcgat aatggcgcgt taactaatcc attacgatat gcttctgctg
    18781 ttgctccttt gcttcctgcg tcgttgtgaa taactataaa cttagggtta ctaccacgct
    18841 taggtaggtc ataaccttta accacatctt tgatgatttt aagttctacc gctttaggtt
    18901 gtggcttagc cgtttccttt ttagatgctt gcgtaggaga ttgtactgat cgtggagctg
    18961 tttcgctttt gaagttagga cggataaacc acatagggaa gtcgtaagcg tgttggcgtc
    19021 ttgtaacttt ttcccaacca gagccaggtt gttgtacacc gtctgtccag ccaccgccga
    19081 gccaattctg ctcatataca atgatataat ctaaagttgc ttcaattacc catgcaacat
    19141 gaccgtatcc tgcaccataa ttactaccga atacaaccat gtcgccaggt tgcgctaaga
    19201 agtctggtgt attttggtat acagtagcta gtccgttaaa atcattagca cttgggatgt
    19261 ctttggcacc tacacctttt aagttgtagc caaataagac ttgccaacct gcattggcat
    19321 agtcaaagca ttgaaatcca taccatccgt ccgcattata ttgttttccc tcagatgttt
    19381 tcaaccactc tataaactct tttttagtta gttttgcttg cattgtcgcc acctccatga
    19441 tgatactcat tcacatcaaa gccaacatcg ttagaggcgt ctgtgaaagg ttgtgatgta
    19501 tcatattctt ttggtgcttt cgtgcttaat tccggcgtta aactgctgtc ttgtgatgat
    19561 ttccacgtaa cttgttgttc ttctttatcg ctatctctag gcgcttgata tgtctgtgct
    19621 atagatgaat ctgagacgcc ttttgacgtt gggtcagtaa taacgccaat acctgtaagt
    19681 aacgtgagga tagcgcctat aattgcgcta gcttgattta attgagtaga taaatctaat
    19741 ccgaataaat ccgtgacttg cttgataaat agcaacaatg ctccaactaa actagttagt
    19801 actgctttgt ttttgaatct caatttccag ttaatatcca tttgtttgct ccttttatcc
    19861 aaaataaaaa aacgactaaa aattagtcgt ttaaaattat tcaatggtca atgtcggaga
    19921 tcctgaataa acatcactta tagtgacata caacgtcccc gaaggattac taaagttgat
    19981 atttttactt gcaactccgc tattgactcc tgatattcct aattcacttg aacctaaatt
    20041 agtttgcgaa atcctcatta taccgctacg tacattttct attgtcacct gataactttt
    20101 attaggttca actccattta ttgtccattt tgctgttgat tcttctatgc tatccggata
    20161 tttattttta ggtaagggtt ttattacaaa atatgaaggc tttttccata cttggatatt
    20221 tccagcatat acttttgtat attcttcgcc ttcgtaaata agtttcttta catttttaaa
    20281 attaccttcc ataaaaatca ccccttaatt aagtaaagtg tattagggtc tttttgatat
    20341 atatagttat attcattttc tgttcctgtc caaattttaa ccgtcggttg agatgcgctt
    20401 tttagttgat ataaattatc cgcttgttgt ttagtaaaag cttgagatga caaaacatac
    20461 cgctcgtcat gattatgatt ttttggagcg tataaatcat ttagtgtttg tttgaattcc
    20521 tcaaaatctt ctgcactaac ttttgagcca atctgttgca atacactttc tgaaatagag
    20581 ttgttttgta ttgcttctgc taattctctt aatgtgttca tagattcagg cgcgctatca
    20641 actagttcag caatttttga atccgtatac gttttagagt cgttgagagt tgtatcttgg
    20701 atttttttaa cttcttgcaa tttatcttct aacccttcaa catttgcgat attgatttta
    20761 tccaataact caggttctgc tttgatatct gtatctttac cgtcaatttg ccacatttta
    20821 gtgtcaggat tgattgatac tacagtaccg tttttaccgg gtgcgccttg ttctcctttt
    20881 ttacctgctt caccttttgc accaggttgt cccggttcgc ctttatcacc tttcgcacct
    20941 ttaaatctac tttcattctt ttcgatgtaa gaaatgacat ctttatctat tttctcttta
    21001 aagtctttgt tcaataaatc tgtcgcgtta tcttttaaga ttctcgtaat agcatcatct
    21061 accaatttaa catcgatttc ttttgctaca gcagattcaa taccactatc aacgatattg
    21121 aaagaaaagt tcgcgacatg tattttttct tcttctttct ctaaaaacag cttacagcga
    21181 acataaccag cgtgtttgat aaccttttta ggtatcttgt aggtaatgaa tccttttaca
    21241 acatcatcga taataagggg ctcatttttg aatatagagc catcttccat aaacaaatgt
    21301 aatctaggtg ttaagccgtg tgcttttaga tcgatacgac cttgtttgtc attgatacct
    21361 attcttatag atgctgtatt ttcatcttca gtgtaaaatc gacagccaat gtcacctaag
    21421 tcaacaccat cattttttat tctcgtttca acatctttta ttttgtacat ttatacacct
    21481 ctttatttat atttatctct tataaagtag atacctttta agccgatttt tttatatagc
    21541 ttagcgattg tacttgcttg atgttggcac cactctatag cagtagcata ttgatgtgta
    21601 gctggatttt taggattcca tctaattcgg tacaatgtgt tttgcccttt gttgatgtaa
    21661 tcctttctta cgaagctagc accgcccatg attgcttttg ctggagatgt ccaaccttta
    21721 ttcctagcaa acgtcattgc gtagttagga ttgttgtcgt aagcgccaat gccgaagtag
    21781 ttgtatactc catcttttcc gttagcgaag ttacttgttc catatccact ttctaagaaa
    21841 gcatgcgcga ttaaataaat ttcattaatg ttgtgctttt tacaagcttc tgcgaacgct
    21901 ttaccttgat tattcaatgt ccccttacct ttaagtatct tattaagcga actaactgaa
    21961 acgccttgat acttgcctaa attaagcatt tggtagcact gcgtgttact ttcccatatt
    22021 cgtttaacat tcattgccga gcccgtttga gctcgtgtag cgttagccca gccccaagca
    22081 ttagattttt tcgggttacc tcttgccatt tgtttatcca gtgcttgttt gaatgtataa
    22141 gggctcgttt cagttataat ctgcggttgt ttagatgccg agccattgtt agctgttggt
    22201 gatgagtctc ttacattcgc tatatcagcg tttttattat ctaccataac ttttattcta
    22261 gattttgtta ctgttggttt agttatagaa tttaataatt tttctctgtt tttaaatata
    22321 ttaagtaatg ccttttctaa tgcttcgtat ttatctttag gggcaacacc gttgtcaatc
    22381 atattccaat taacatgttc caacatagaa cgccaaatgc tgtcgtctac ttttaaattt
    22441 tcaatactta gaggtatctc atatttggcc atcatatcta cagctacaac cattgcgtga
    22501 atctcattaa aaataaattc atttttactc gcactataat cttcacatac gtctataact
    22561 atataatcag gttcattagg aacttcaaat acagctcttc taggtgccca aatattatgt
    22621 ctatcaacat aaaagtgggg atattccaca tcttgtttgt atttcttcct actgttatat
    22681 aaattttcta ctgaactcat tgtttgagca tttctaatca ttatcccttt aggtttttcg
    22741 agtcgtcgat taccctctac tataaagtga taaatatatt ccggataatt aacttcttgg
    22801 ctagaaattg tgtactttat agttgttaca tctttccaaa ttggaacttt tttattattt
    22861 ttttcgttat catcactatc atcttcgggt ttaggtgccg gcgtagattt ctccggatga
    22921 tatggtggtc taacaaaata tttaacccct ccacctggtc catcatgata agagtgctta
    22981 attttatatg gcggacttcc tgttgcgtta tttgtatacc agttttgatc tacgccatac
    23041 caatagtctt ttgtgcatgg tcccactaca atgtttacat gtcctgccca accaccagtc
    23101 caaacacccc agtcgcctgg ttgtggtaca aaatcttttg tatttctaat tatcttgaaa
    23161 tctctacctc tataattgga tttttgagcc atagcatcag catttcccca tgttctaaac
    23221 ccccaatatt tatcgagtaa ataattaggt aaatcccagc attgtgctcc cattccagaa
    23281 ccaggtacat caatagctat tttgttttta gcgatatata acgcccattc aaccacttca
    23341 ctagctgtag gctttctagt ctttggatta ggtaatccca tgtatgcacc tcatttcaat
    23401 caaaataaaa agccagtgcc gaagcactga ctcttaactg ttatttacat ttaccaaacc
    23461 agaagcacgc ccagaagcta tatcctaaaa tccctttaag catggtaatc acctccttta
    23521 aataccaaaa atagttctta gtaaagctat gacgatcgta ctgaagatag tccctaccaa
    23581 accgagaatc cacattttta tgtctctaat attcttggca ttcttttctt tattcttttc
    23641 atcttctacc ttgtcgcgct ttaattcttc aaaatttcta tctaatttgt cataaatctt
    23701 ttcttgcgct ctaagactat cttctattct gtcgaatttt tcaaacatag tcttatcatt
    23761 ttcttctaat cgcgttaaac gccaatcttg ttcatgtcgt ttggtaaaac caaacattac
    23821 gccacctact ttttgttaaa ttaaaaagcc acaagcatta cacctgtgac ttttcatctt
    23881 ttgtttctgg atatttttct ccagtgatca atgcatattc ttctttgtca attacaccca
    23941 tgtctacgta ccacttaatt tgctcatttt tatagcaacc ccacacataa aaagttttaa
    24001 tgtctttaaa agttggataa atcatcttca tcatttaaac gtccccctca gtatttgttt
    24061 tgttagtttt cagttcggtc aactgttgtg ttaacatagc gttttgttgc gtcaattgca
    24121 ttgtcaacat gttcacttgc gtcatctgca tttgcatact tgcaaccatt ccgcgaagtt
    24181 cttcatcact caaatctgat tcactttgtt gttttgatgc attcggtacg tcttcttttt
    24241 cgaaattgct attgtattta atttcgccgt tagtgaaaac aaactttcta ggttcgaact
    24301 cttctttaaa tttaataggc acattgttat catctacatc taaactattg cgtaatccgc
    24361 cagtattaac gtatccgata acttcgtttt tatcgtttac tgtgattttc attatttcca
    24421 ccccacaatt ttatttatcg taactctgtt tgcattagca ccagaacctg ttttactgcc
    24481 taaatcaagg tacacatcgt tatcgatttt taacgtcgta ccactttctt tagttattaa
    24541 gcattcataa ctaccaccac cgttaccgtc tgagtcaact acatttgttt tacttaattg
    24601 aatcgcattt ggtatagagg ttaaactgaa tgcttcaata acaccacctg gataagtacc
    24661 gcttatgaat agaattgcat aatttgtata agcttcggtt aaattaatcc ttgttcctac
    24721 accgtttgca gcaccgtcga ataacacggc tgttttatgt tcgttaggtg cagcccattg
    24781 tgaatctaat cgaccattgg tgattgatcg tgtataaact tttttagagt ttgaaggtgt
    24841 gaagttgaat aacttatttg catcatcttt aacaaatact gataagtagc cttcgtaact
    24901 ttcaacaata cctggtaaat caggtacact tgttacgtaa taattcccag cgcccaatgc
    24961 ttctaaatta cctttggcgt tatataagtt cttttggatt gattgaccgt tatgttctgt
    25021 taatttatgt tgttgccaac ttgtactttg aaacttacca tctacatact gtttagcttg
    25081 atttaaagcg ttgttagata tttcttcaac aaattgctta gttaagtttc catcattctt
    25141 tttataaaac gggtaccacg tgccgtagat tttgtatttt gtgtactcat cgtttgaatc
    25201 atctgggtac catgttgcac gagcagtatt attatcaaca acataaacaa ctaacacacc
    25261 agatttgctt gatgtataag ttgattcatc gaacgaagaa ccgtcatcaa caccatcttg
    25321 tccaggcttc tctaacgtgc ctatatccgt cttttctggc gcatctgttg cgttagtaat
    25381 atgaataatc ctagatgtgt taactgtgct taaaacgcta tctatggact gctcagacga
    25441 ttcaatcgct ttgccataat catcagtaat cttagacttt tgccaattga ctgtcgagtt
    25501 gcctttgaca aggtcagcac cattgatttg ttgctcaact tcattgacac gcgcaaatat
    25561 cgtttgctcc ttttcaacta ttttattgaa ttcagctgta acagcttgtg ttgcactagt
    25621 ttgagtcgca gtaatagctt gtatagcttc gttttgcttg atttcgattt gttgaatgcc
    25681 ttttgtcgca ctatcattca cttttgctat taacgtttgt gtatcagcca tattttgctt
    25741 taattggtta aagtctttac cgacagcttc gatagtatct tgaatagatt tgatataaac
    25801 aagctttgtt atgccatcaa acccactgac taaatcattt tcaatattga aactaaattg
    25861 acgttcaaca actacgttgt tactcccgtg ttgcgtgaag aatgcctgag catgtacctt
    25921 acctgaatgt tttaaaaatt catttggaat cacatattgc aagcgcccat taattgcgtc
    25981 tactaccgtt aattcgtctg aaatataagc gcctctatct acgttataat catcggtttt
    26041 taacacgatg gaagttttaa catgttcaga acttatagat aacggtctgt tattcttagt
    26101 tactgcaaaa tttaaaacac cagttcctct atctgattca tagaaactga tgtttgtgtc
    26161 aataactgga ttatattgtg atgttgtttt taactcgatt aagttatcat cttttgaaaa
    26221 attatctact accattattc aacctcctta ccttctatta tgctccaacc actattacca
    26281 ccagtaccaa agtttctaac gaaaaactgg tgagcagaag caaagttatt acgtcttagc
    26341 acttgtgttg tgttacccgg tgtatttgat tttacttcta acacccagcc tgcaatacct
    26401 ttgtaatctt tagggaagtc agaaaaacgt tttgattctt cagtggtgat atagaagtct
    26461 aaaccaacaa tttttaaatc agacaatttc gtgatgctct tagggatatg ttcccaataa
    26521 ccagcacttt gtgggttaaa attccatgaa ccgttgtttt tcttgttaaa gatgtcgata
    26581 acacgttcaa atttgagcat atttctacct gtgctgtttc tagttagtac ttgtcttaac
    26641 gcaccattat aatgaccagg cagtacatca aagaaccaac ctgcatctct aaacgctttc
    26701 ggtaacggga aatctaacgc attttgtgtg tcttgcgtat agatatagta atgaccaact
    26761 tccgtaatat cacttagata tgctgggttc tgtatcggta acggtttaac acgtccgcct
    26821 gaatcagtca ttgatacttg aggtgcaatg ttttttaaga attggtttac acctctttga
    26881 ccgatagaat aaattgagtg atgtctgttg ttacctggtc caatagttac cccaattaaa
    26941 agtgctttac gtcctgtttc tagatcgtaa tacatatcta gaccctcagc ctcttggaaa
    27001 tctcctttaa agttgttatt cacaccgcct atatcgatac gacgtttaaa taataactct
    27061 tttgttttga catcgaagcc ttgtaagtaa ttagggttgg ctgtattcga atcacctgta
    27121 taccaatata agatacctgc atcataagtg ataccttgca taggttgtgt atctgaagtg
    27181 tattccatag gtatatccat ttgatacaat actttgtcta tacctttatc aatatcgtca
    27241 gcacttctta cttcaatgaa attcaatgaa ttcttagctt gtctttcaga agctttatat
    27301 tcacgtctga aaatcattaa attttctata ggattataaa tcgctgacgt atatctgtcg
    27361 ttaaatatat tcggcatgac gtcttgcatt tcattaccat aagttatttc tccagttcta
    27421 tattggaaac gtacaaactt gttgtttttg ttactgtcca atacagctga ataaatccat
    27481 aattctccat caatgtatct atacgcattg tgtgtaccgt gaccgccgtt tttaacaagc
    27541 aatctatcaa taaattgtcc attaggcttc aatctagata acatgtaatg attgcccgga
    27601 cgcgcttgtg tcatataaat aattttcgtt ctagggtcta tccaaaatga ttgcattact
    27661 gcgttagtat atggcgataa atctgtgatg aattccggtt cttgctcttt tggttcaaat
    27721 cggtattctg tcgcttgata ttctttatag tgttcatcta cagctttctc aaccttttta
    27781 gtgaaagtat ctagtgttga ataatcatga tacaaacgat cttgcaatgt cttatgatca
    27841 taaccagtat tatcaacacg cgcgtctttt acttcgttga taccgtcgcc gttatgacct
    27901 agtaccatat tgctgaaacg gccgtttaga tacgttaaaa aatcagagac gctacttgtg
    27961 acatttaaat gctcatactt tatttgctct ccattatgtg caaatacctc tttatttcta
    28021 tggtattcaa gagagaaatt aaaatccgtc agcatgtctg aaataagttt aaagttatac
    28081 tcattttcat ctacatatct gtagtcaaag actctactta agtctgtaat taatttgtta
    28141 tccatgtctt cctccttttc tatccgtaaa actggtaata atttttaata agttcgtaca
    28201 taataacttc atgacccctc tcgttcggat gcaatccgtc tggcatactt gattttctga
    28261 acgctggatt atatggctta aaataatctg tatgataggc atcatatact ggtacatcca
    28321 attcactaca agccaatatc tgagcattga cataatcctc taaagttaac cctagtttgt
    28381 ttttgtccgt atctttacgg cgtatcgttg taccactcat agggcattgc ctagtagctg
    28441 tcattacaag tatttttgaa gctggattat ttttcctgat aacttcaatt gcagaacaaa
    28501 aggcgccgta aaacgtttta gtgtcggttt tatcagtgcc tatcggtacg cctgcccaat
    28561 aaccatgtaa ccagtcatca tctgtacctt gtaatatgat taggtctcct cttatttgct
    28621 ctgcttgtct ataaatgctg ttttctaccg cttctttacc tattggaact gttgccattg
    28681 ttgcgccacc tcttgcaagg ttggtcgttt tagcttttaa cttcttgcct aacatttctg
    28741 tgaaattagt tttcgcatgt gatcctctag ctacagaatc gccaatcgtt ccaattgttt
    28801 ttacatcttt aatgtttgat ttatctataa aatcatgaac gatagtgcca tctgatgtag
    28861 ttacagtctt agaactcact ttctgttgtt tgtcttcaat tagatcagtt ctactcatca
    28921 aatcgagtgt tgatttagct attgacgcta ctttagactt caagttttct gccgctttac
    28981 taggattaga aaggttaaca tcatttaatc cagaaacata gttagctgca gtattaactt
    29041 ttttcatata tcgttgttct cgattaaact caccaagcgt tacatcttgc ttaacaatta
    29101 cattgtttat acccctaatc gttttaactt gtactatacg gactaaatca ttcaaaccta
    29161 gtttggtaga ttttatttgt actatgtctc cgggttgtgg gtctgcttct ggatatgatt
    29221 ctcttaacac caaaaagtcc aaagacaaag attgttttaa cgactttttc aatctcgatt
    29281 gtaattcttt atccatagtt tcttggtcag tcactttacc atctttaaat ggttctgcgt
    29341 ggatgtcgcc gtatatttca gctaatgcac ttctagcttc cattacgagc ccagcgtgtt
    29401 cgaatgtttc ttctcctgaa taattaccat atcctctaat gaaggtggcg aaatcacttg
    29461 catcttcctc gagttttata gcgttggcgt tgacttcatc agaaataaaa taagacgctt
    29521 tttgatttgc aaaaggcgtc aatacaaact tatatctgtc tttctttttg tcatacgtta
    29581 ttttatattc taaaccgaaa tgttctaacc cctttttaaa catttctaac cttgtgtcgc
    29641 cttcaccacc attttcaaac ttcgaagact taaccttacc ttcgacttca aaaagcattc
    29701 cagtaccttg aaacacaatg ttaaaatatc tttctactgt aaaagatcct gttacattaa
    29761 cataaatcct atcaatcatt aacttgtcta taggaatctc tctagcagta cattcaacca
    29821 gttgtctgtc gccttctgat ttcctatcaa tgacagttat tacatattct ttcttgtcat
    29881 tttcaccttc gacatgacta acaatccatc ttttccctat agcgttaata acttcataag
    29941 tgtatttgtt ttcgagaata tcaaaagtta atacaccgtc agcattaact tttttcacta
    30001 aagttgtttc tactggtatg ggtgcgccat tgcctttagg tggtttaaca gttatcgtca
    30061 ttctgacacc tacttataat aaaatttcaa atcgaactga accttttgaa ctgtttggtt
    30121 aaattcgaat ttattagctc cgtatttaaa ttttggttgg gctatgttcg tttcggtact
    30181 tatttcaaca ccgtttttat aaactcggaa gctatcataa acaattttgt ctccagcttt
    30241 tagtttgata ccttcgattt tcattatttc agcatgcgtt aaattccata caaacgattc
    30301 tgtatcttcg cccaaaataa ttgttatctt tttatacatg ttgaattggt cgttaggagc
    30361 actaccatga tagtaaactg tatctttgct aacattttca aatgtatact ggcgcttatc
    30421 cccacctgca tgccaatcaa tattaaaatc aaacgaccac aatccaacct ttttgttttc
    30481 ttctaactct aggcttgttc caatactttc gccgtatggt aattctgtag tttcaaactt
    30541 caattcgaaa gcaactttgt tacctttttg tttaggattt ataactccgc taaaaataac
    30601 tttatactgt ttaccgttta cataaatttg ttgatcgtgt cttgaatatt cataatccgg
    30661 gaagttgttt ttatctaatt tcacgtaatc atcagaagtt ggttgagtaa acctgtaatt
    30721 caactcttct tttcttctga tttcacgtaa atacattggt tctatatcag tagttaacga
    30781 atacaacata tctcgcatat aagcaatgtc tgaacgattt tttactttac aaaaacaagg
    30841 aacaactata tctctactga tataattgct ccccattaat atgcgaccgt tcatattttc
    30901 tttgtcttga tactttgtgt tgatttgcat gctatcaatt actatatcgt taacgataaa
    30961 cccgtattca cttaatttga ttacagtacc atcttttttt gttaattcta tgtccatttg
    31021 taacctcctt tataagtaat actcagaatt gcgtttagca tttctgccgt taacaatact
    31081 agtaagcgca tcgttattga catcgaattc aattttaaca gttttcatgt tcggtgatgt
    31141 ttcaatagaa tgtgtgtgtt gtacttgcgc atttatattt ccacctaaat tacttaagtt
    31201 tcctgtaata ctagaaatgt caggtgcgtt taatgtaggt tgaaatgcat caactacttt
    31261 atctgcaaca ttagaaacat tacggataac tttacttgaa tgattatcta tacctttaac
    31321 gaaacctaac attgaataca taccaacatc catgaattca cgtgaaggtg agtgaatacc
    31381 tagcgctctt ttggctgcat ttaaagcacc ttttgctaca ctagctgctt tttcagctaa
    31441 gtctctagcc atattaccaa tacctctcat caaaccacgg atcatatcag cacctgctga
    31501 tacaaagtca tccacaaagc ttttaacttt atttactgca tttgtcatac cttgactaac
    31561 tttgtttaca acattaacga atccttgaac aactctatta acaaagttaa ttagcgtact
    31621 tgttatagta gatacccatt gcataccttt agtgacaatg aagttccaag cttgagacat
    31681 tttgtctgat atagttgata caacttgtgt gaatatgctt acaactttat tccaaattgt
    31741 cgttaatata ccagataaga aactccaaat cgtattccat atattagaaa taaaactcca
    31801 tgccgcttgt aacgcagtag atatagttgt agtgatagcg ttccaaacct tagttgccac
    31861 agtaactata gtgttccaca acgtttgtaa gaacgtccaa atagcgttcc aaattgtcat
    31921 tgcgatagtc ataattgtgg taaatactgt agttattaca gtgactaaca aattccaaat
    31981 cgtagtagcg attgtaatta tcgtgttcca gattgtactt aagaatgtcc aaatagctgt
    32041 ccatatcgtc ataactattg tcattatcgt cgtgaaaaca gttgtgatga ttgtaactaa
    32101 aaggttccat actgttgttg caatagcgat aattccattc catagccctt gcaaataagc
    32161 aactatttga ttccaaataa tcattataaa attgtataca tttgatactg ctgtagtgat
    32221 agcttttaaa atagcattcc atacaaccga agctacagtt ttcaacacat tccaaactgt
    32281 aaccataaat gtttttatcg cattccaagc atttataata aagtttctga atccttcatt
    32341 tttattccac aataaaacaa atatagctat taatgcagcg attacaccaa ttactattgt
    32401 tattggacca cctaaaatac caaacacagt tactagtcct gtgatagcat ttctaattaa
    32461 tccaatctta ccgaataaca attggaatat aactgatata atttttaatg gtccttttaa
    32521 taacatgaac gcacctttta aaattgttaa tcccgctctt aataaaccga acttacttac
    32581 taatgcaatg attctaccta ttaatccgcc acccataaag ttagatacag caagaataat
    32641 cggtattaaa aatctaaatg caccaactaa agttataatg acaccaacta attgtgctgt
    32701 agctggatgc gcctcaaaca agttagctat ccaaccagtt attgcaactg caacgcgtaa
    32761 tactgcacta gctataggag ccattgctgt tgcgaatgca actaatcctc ttgcgatgtt
    32821 tccaatcaat tgcattatta gtggtccatt tgtttgtata taactgacaa agtctttaaa
    32881 cccttgagat tgtcctactt gttcagacca ttccctaaac ttagctgtca tttgttcaag
    32941 agattggaat atgccagttg atgatccgct gaatgcattc atcaaattgt taattccaac
    33001 gaaaacattt ttgaaaatat taccaatgat aggtaagttt gtttttgtgt attcaataaa
    33061 acgagttatc gaattttctc cagctgcact attagcccag ttagagaaag attgacctaa
    33121 tctatccaac caatcagccg accattgaaa cagtggtgct aattgcgtga atacattgac
    33181 taatccgtca ccaaaatcac ctgcagcact taatagcttg ttaaataccg aaacacccgt
    33241 tgtattcatc atattaaaga atcttgaagc tacactgcta ttttcagccc atttaagcac
    33301 gctttgagac gcttcttcca ttcctcttga aataccacta aaaaacggtt gtaagctctg
    33361 cattgcagtt ttaacagtat ttaaaccatt tgcaagagtt gtgaagatag cggattgatt
    33421 ttgctttata atatcagtcc atgctgactt tacgccatct aacgcttttt tgtattcgtt
    33481 tgttgctgag ctagcttgta aagtgccatc attaagcatc tttatagcgc tgatagccat
    33541 tgcgccaaac gctacaaatc ctgctcccgc tattgctacg gcaccaccta aagcaagtac
    33601 accaccagtt aacactttga tagcgtttaa tagcgcaaat actacaggta ctacgctcgc
    33661 tattacaggt attaagatac taaaagatga tgtaagtaat ccaccaacca tattagaacc
    33721 tacagtaccg aacacacgga acatattagc taaattcccc atctgtcttt gaaaattgtc
    33781 atttgctttt attatgtagg cataagcttt ctttaaacca ttagtatcga catctacctt
    33841 tgttgttttt ttgttcggca atgcgtctaa tgatttttta aacgcataaa tagttggtat
    33901 agaaagccct gtatctacat ctagtcgaga tctagttttg tttggaatac ttttaagctc
    33961 ttctttagtg cgtttgattt tagagttagc aacaccgttg tccacgtcta taatagcttt
    34021 ggctttagac ctatttaatg cttcaagact agctttagat acttttaaca ctcgattgaa
    34081 tttactgtta tctgcattga tgtcaatatt gacacgcttc ttttctagtt cggataactt
    34141 agcttctgct tcagcgatat ctttagtcaa tttttgtttt tgtaatttaa tctctggagt
    34201 aacttcttta gagtttagtt tgtctagttc aaaattcgat tctagtacct tttgttgcaa
    34261 gtcttgtata ctagcatcta atttagcttt tacttttttg ttactaaagg catctaaaga
    34321 ctttttagca actttgatag ttttttgtaa ttttttatca tcagcattta attcgacatc
    34381 tttagtttga tctgctactc gtttaaatct ttgcacagac ttaaccgcac tatcgatttg
    34441 ccttttgaat ttggctacac tagcttcaat agtcgcttta attttatatt ccgtcacatt
    34501 aacacctctc tttctattgc ttgttaaatt ctgctataac tttaaagaat tcattatttt
    34561 gtggttcgta ttcatcacgt tcgctactaa atcttatatc tttaccttcg ttaagccgtt
    34621 ggatattttc ttcataaggc aatacgtcgt ttgcgttgtt aaaaacatat tcctctttag
    34681 gtttattttc tgtcccaaca tttttagtag ctgcagcatc acgaatagca aacgcaagtt
    34741 tgtaacgttc gaattcttgg gttagcattt catactcttt cgcatacatt cgatagttat
    34801 attctgttaa tgtcatttgc tcaataacgt tcaaatctgt aataccaagt gttgacatac
    34861 aagttataac gattctgtcg taagttatta ggcttccgct ggtttctctt ccgtttccac
    34921 tacttcgact aggtttcggg tcataggtcg ctttcccaac tccgttaaaa tatccgaacc
    34981 aaattcttct agtccaatat tttctgcgat ttcatctagt gcttcatcaa tgttattaat
    35041 agtaattgct tgttttttca agtgagatgt agctgcaatt aaaacttcgc caatcacaac
    35101 aggatttcca ctctctaaac ctacaggcaa cattgataca ccttgaccga tagaagcttg
    35161 ctcaactttt aaacctaatc ggttatcgat ttctcttaaa aatttaaaac caaaacttaa
    35221 ttctaatgac tttccgttaa tttctacatt cataacttaa aatctccatt catgattaat
    35281 ttaaacaaaa taaatagggc ttaacgccct atttttatac ctctcctggt gtaaccgttg
    35341 atgaatctac cttaggttgt ggaattgctg ttaaatcttc gccagttaac gcatctgctt
    35401 ttgtagtgtc atggaatctg tatccagtcg ccttaagttt ctttgttaca gcctcaggta
    35461 gtgttgcaaa tccacgttgg aaacgaccat tcactccata ttcatattca tattcatcaa
    35521 taccgttagc ttctgctttt aattcaaatt tattgtggaa accttggaaa tatttcgctt
    35581 taaatttagt agcatctcca tttttgcctg gtattctact ttcaacttcc caagcctcat
    35641 acaatacgcg atctacaact gcatcttcaa tttcatctgc aaaatcgtca ccataaaaca
    35701 ttttagcagt accagacatt gttgattcaa ctgaaccacc agtgttataa gacccatcca
    35761 tcgtatcctc tgtatctgta tcagcttcat gtgataagcc gtattcagtt aaaaaaagca
    35821 ttttagtagc atctactttt tcgccagctt ttctaaacaa aataatacgg tcattactat
    35881 ttttcatatt cgccattcaa tattcctccg ttttttaaaa tgttttgtaa gatatcgtta
    35941 ttgatgtgtg tagcaattct tgattagtag tatcatcgac taactgtgcg atgttagtat
    36001 catcttcttc aaagtcataa tcgtttgttt taacgctagg tgttaaatca tcgatacacc
    36061 ttttaacaag tccgtcatga tgtcctaaat catcgcttac actccaaata tcaataacta
    36121 aattcgtatc gccagaataa ctatcaaacg tgtacttact tctatttgac tccggcattt
    36181 ttattacaaa aaaaggatac ggaatctctt gttgcatctc tttacgagaa ataacaggga
    36241 atccatatcc ttgtagcgtt tcatacgctt tattataaag ttgtaagttc ggtgtcatgc
    36301 ttttatctcc tattcaaaca acgctttcaa ttcttctaca gttgatttcc taatcacttc
    36361 gtataccggc cacataaaag gttcagcctc catgtatcga gtaccaaatt ctaagaaacc
    36421 actataagct gcatgcgatg tgatagtgta ttgcaaatcg ccagtttttt tatatctgat
    36481 attgcgtgat aaattaccag tccaataacc cttattcatt acttctctag ctttcaattt
    36541 agctcgtact acatattctt tggcgttttc ctgtaaaata tcatctacat catcatcaat
    36601 gttggttttc atatcgtgaa attggtttaa cagtgcgtct aatccatcta tattcatcaa
    36661 ttgacctctt cgatataata tgacgtttcg tgtctgtata tccttgtatc aactatcttg
    36721 tagcgaatgc cattaaccaa cacgtggcta acagggtaag atattgattc ttttatcctc
    36781 agaacactta catcgttttt tacatcacca aattcaagtt gctttcttgc tctagaaatg
    36841 gggttaatat tgcatggtat cgcatcataa gtgattagtg tgttttcttt tttgctagtt
    36901 ttaggattgt aagttgctac ttgttctaat tgaaaaataa ctctatcttc atatctcaaa
    36961 agaacacagc ccttcctttt ttagttctcg ttctagcatt aaagtaatta tcaataatag
    37021 cttcatactc cttgaaatcg ttcaattcat acgcattgct acgtccgtca accgcttctg
    37081 atgtcatacc ttcagcacca atcctgttgt agcgtttaac tgcaacttct ttaatcatgt
    37141 aactaaacct ttccggtatt tgttcaactt caataggtaa cattgataac aactggcttt
    37201 cacaactttt tatgatttct tctaattgtt catcttgctt ttcatcttta agaccaatac
    37261 gtttttttac atcagctagc gtagtcatat aaccacctac tctagtgact caaaagcatt
    37321 gataatttca gcttttgttt gtttttcatc aacttgtaag ccagcaacac ttgctatttc
    37381 gacaagttct tttttggtta atttgtcatt tacaatgtaa atcatttgtt tgttgcgttt
    37441 attttcaaca ctagctaaag ctttgatacg ttcatctgta ggattataac ctttacgagg
    37501 gtagacatgc cctttcatat agacatgtct gttatcttct aaatctgtaa aatctacttt
    37561 aacaattcca atgatttcgg gcatgttacc actcctaatt atttattaaa cttctcctgg
    37621 aactgaatct gttcttttgt cagcaggaac taacttagca aacgctttat catcagcaat
    37681 atgcaatgct acatgcatag ttgcacgtaa tgccaccatg tcttgttcaa acaagtttac
    37741 aggtgtgcca tcttcgtttt taactgtaga taattgtgca gtttcatcga ttttgtattc
    37801 gattaattga gggataccat aaatcaattt gtcgaagtca ccagtgatta attcaccacg
    37861 ttttaagttg cttgatttaa ggttaaccac aggtagaccg tctaacgaat cactgttacg
    37921 gtcataaata cgttctttcg tttcaggatc tacaatttta cgtaacaagc ttctgttttg
    37981 tgtttttgag ataaatgcat ttgcttctaa ttcgtcatct tcaagcaatg cctctaaatc
    38041 aataatgtta tcttgtgtga agtcaccttt aataacctta ttagtttttt caattgattg
    38101 tgcaattgat ttaccgaacg gattgttacc ttgattcaaa atacctgcct cgtcaaactt
    38161 tttatagaaa gcttcagcaa tcataggttt catttcttca aagaattgtg aataagtgta
    38221 attcaagaat tcttttgtta ctggtaagat aacccctaat ttaaacgctc tcattgtagc
    38281 attaacccaa gtagccttag acgtttcgat tttttgacct tcacctaccc agtaagcacc
    38341 tggtttatca gcccaaaaag taaacttctt ctcagtacct tccattggtt cgtacttacc
    38401 taattgcatg attttagagt tttccataac ctcttgtaag ataggtgttg taaagtcgtt
    38461 taacaacgtg ccatctttct tttcatgcat cattacattg tcagggttaa atacttgtgg
    38521 tttaacattg ttacttgcaa aatgttgcaa atttaatttt aatttttgtg tttgttccat
    38581 ttaaatgcct ccgttaattt ttaataattc ttttttgtct agctatttca gctaagtttt
    38641 gcggtttatt tttagtcgag tgattaaatg aatctccacc agtcaatggc gattgtctag
    38701 cgttaacctt aaccgcttca ttaaccgctt tttttactgc attagaaaaa gcttcaacat
    38761 tcaatttagt ttgttcagca gtatctgtta caactaaatt gacaacctca tctgatgaat
    38821 caacttccgc ttcactcaac atttttcgtg cttctgaacg catttcgttt aattgttttt
    38881 ctgaacgtaa ttgttccagt tctttttcca tttgttcgcg ttcatattca tctttttgat
    38941 ccttgttcat tttcgctaat ttagcagctt ctttagcagc ttctgctgct ttttcttttg
    39001 catactcatc agcttttttc ttttcgtggg ctacacgacg ttcaagtatt tcatcaactt
    39061 tcttttgttg ctctggcgtg aaagttattt cagtaccttc gtcattttct ttcttatcag
    39121 gatttccttt tttaccatct ccgcctggtt cgtccggatc atctgattgg tctgcaaaaa
    39181 attgcaaatt aaacttaagt ttattttctt ccatgagata tacctccatt tatagtctgt
    39241 cgactgtttt tccatgcgtg ctttttatgt catcagcacg ttttggacat aaaaaatagc
    39301 caacacaatt aagtgctagc tattaaaaga gaggttcatt atatttcgat ttttctttat
    39361 cggctaatac tgccgacctt aaactgtcta agtttgcatc aataataact gtttcgtttc
    39421 gcttttgtaa ctctttacgt atacctttta attctcttgc tatgtctcta aggtatttgt
    39481 cagtattact catattagta tcctccaaac atttaattta ctgtcataca aaactaactt
    39541 gcctttaaaa aacttcactt ttaaatcaat caccgctttt cactttccct ccgaagtatt
    39601 ttgtttttcg tttcttgctt ggttttttcg gccacataga tttaggtagt aaagcgcaat
    39661 ctgaacgaca attgatatgc ataggataga aattaacacc aattttagcg tctttaactt
    39721 tgaatatttc tccattaagc cctttgcata ctttagttgt tctattatcg atttttgcaa
    39781 tatacatata atatccttcc ggtgaaattt ctttcatgct gtcaatgctt gattgtgcgt
    39841 gaacacgtgc cgattccgta taaagcaatg atttaattgc tgcggtcttt tgtcgtgctg
    39901 tgccttcgaa tttatttaag tgcttgcgca tatctttaac atattcatta ggatgtcgac
    39961 ctctaataac tacattagca attatttctt ctacttcttg tttcattgct tcggtattag
    40021 tccataatcg ctctgaccaa acgacaccat gaaattgtgt atcaacgatt gtatctataa
    40081 cttctttagc tacttgtaca ccttcaccta aaatacccgc ttgatcactg aacacacgat
    40141 aagctgttga ttcgaaatat tccctcatcg ataattctgt ttgagctgtt gcataagcaa
    40201 ttaagaattc tatttgaatc tttaacatct gttctctaga tacatacatt ttcgtgttat
    40261 acttctttaa ttcttcattt gctctatcgc taaactcctt gttttcgacc aatctttttg
    40321 cttcttcttg aaacgctttt acatcgaact catcaataat cttttgtgct tcttgtaatg
    40381 taacgcctgc aaaatctccg tacttgacaa taaacgcatt gatctctttt tcaatgcgct
    40441 taatcatcat attcaatata cgttctattt cttcagcttt agttttatca cgcttcaact
    40501 cattctcgat tgctttgcgt ccgcgttctt cccaatattc ttgagtgttt ttgttaggca
    40561 attacaatca ttccttttta tcaacagtat cttttgtatc atcatcttgt tcgtcatcat
    40621 tgatgtctct agggtcttta taaatacctt tttgagcttt tttaatagat tctttctcat
    40681 cttcttcgat tttcttaact tctaattcag ggtcttggaa gaacgagaat agagacatta
    40741 aagttgtttg gctaatcttc ccaccagaat caatataagc ttttaattct tcaatcaatg
    40801 atttaggtaa gtttctgttg tatacgtatc taacagtatt gaaatctttg ttagcgtcaa
    40861 tcgaccatgt atttttaagt attgtctcta acaacttagc acgacgtctt aaccctttag
    40921 taaacaatcc ttctttagtt ttagtacgtt gttccaatcc aaataatttg tatttcattg
    40981 cctcgcccga ttgagtgccg ctaaagttat catctttcat gttaggcgtg ttggtaaaca
    41041 tgtgtatatc actgtttaaa cggtctttat aagcttcggt accttgtaca tcgtattgct
    41101 tataaatata accaccatca acagagcctt ctgtttctct accttcgcta tcagcataaa
    41161 cagtcggttc taaaaacaac acgttagctt ccttttgttt tctaacttct acaggatcta
    41221 aatttaaatt acctttaata agtaacatag cgtcatttaa atcactcata tagttagcag
    41281 tatctgattc agcattatca tacaaatcaa ttaaagtgat tactttctca taatcccctt
    41341 ttcttctttc gttgttgcta aattctgtaa taggcatacg ttcgaaagag tgtgattcaa
    41401 aaccgttttc acgtggtgtg agcttcaatc catttgttct actggtaaga tatctataaa
    41461 caccgtgtga agtgaataaa tcaactgtaa acactccatc ttcgtcagtc ttgtctattg
    41521 gtttagttct taaatatcta acgcccgcga tactattacg ttcaattgta ttgtcgtata
    41581 tgacaaaagt actcattgca tcactcttgt ataaacgcgt ttcatcatct tggtttctaa
    41641 tcattaactc ataagctttg ccataaattg acaaatctaa tcctaaagat ctattgtgtg
    41701 actcaacatc atttaaatca ttgaacgcct caatagcttc taatacatct ttgtcatcat
    41761 cttgatattg aattggatta cccaagaaat agccgttgat aaaatcgcta atataagatg
    41821 cgtaatcatg cgctacacgg ttatctgcca tgtactcttc tttgcgtcgt gttaactcaa
    41881 ctaagttctt agttttacct tcgtaataat cacttaacac tttcaatcta ggtcgttggt
    41941 aatccatgtg atgttcaatg tatttactta cttcattaac gttttgtaat aaatcggatt
    42001 ccgtcccgtc atatgtgtaa acaacattgg cttcatcatt aaataagtaa tttatgtttc
    42061 cccgtagatc tgtatctgtt tcaaattcgt ttacttttaa catttgttcc ctcctataat
    42121 cctagagatt ttattgtgtc aactttcgaa ctgagatttg tgcgttttct aaccggtctg
    42181 tagaatcgtt ccactgaata acgcaacgaa tcgatacaat gattgtatgt atctactggt
    42241 tcattggtat attcacctgt atctttgtcc ttttgccatg tgtagttgtc aaactcttca
    42301 atagtcttga aacaacgttc atcaacaatg atttcaaatt gcattaagaa ttgtaaccct
    42361 tgtacaaccg agcccttccc ttttttggtt ggtaaaatcc ttttaagccc tagattcctt
    42421 aattcagcta tacttttttg ttctgcacta tctgctgtaa tttcttcttt agcataacca
    42481 agttgcttta tgacattagc tatttcatca ttcagcatac cttgtttaac atactcttca
    42541 atgatgtata acttcttttt ctttacatct attttagaat gtataaaagc actaggatca
    42601 ttaacgtagc caaagtccaa tccaaaataa gaaggtaaat gtcttaactc atctttattt
    42661 attaaacgtt tttcatactt agggaaaacc aatttgtcta gtgtagcaaa ttcacctaac
    42721 gcataaattt tgtaatatgc tggattacga tttgctaaca actctaagtt ttgtcgtgtc
    42781 atttcatcaa gaaacttatt atctcgataa ctagattgtc taatcatgac attttccatt
    42841 ggttcaccat gttcaaagaa atacttataa acccaattca gtttagatac tgggttaaac
    42901 atcaaaaata tttgcttatt cacgtgttta cgctccctca aacgcaacgt taattgcgtg
    42961 taatcattta gtgtgaattc agacgcttct tccatgacta tgtctgatat gccttttatc
    43021 gactttattt tctctgggtt atctaatcct ttaaacaaaa aaactgcgcc gtttggcaat
    43081 tcaactttgt tatcagtctt attccaaagg cacatgtccc aaataccaaa gtttatcaaa
    43141 caatctttaa catcttcgaa caaactatct ttaattgttg attgtacttt tctaagccac
    43201 agtatacgcc taggatattt ccaatcttgc aatgctttga gtacaacttt ttgtataacg
    43261 ccgtgagact taccgctcga acctccaccg taatgtactt cagtgaagtt atcgtaattg
    43321 gttagtattt cgaatatgtt tctattgaaa acattagacg gtttgttaaa gtttaattta
    43381 actttcgtca tcgtactcac caatattaat ctcaatattc ttctgagtaa tttctttttt
    43441 atcgatatac gcaccatgta cttttagtat gtggtcaata gatctctgac gctcttcaaa
    43501 agttggtgtg attgtgtaag taacctcttt ttccacttca tcgtttaaat ggtcatattt
    43561 cttactgtaa gcctcttgag gttctcctct agcaatagaa gcagataacg ctaaagcttc
    43621 tgtaatactc attaaacgct cttcttgtat ctgttctaat cgttctttaa tatattccga
    43681 aacattaaca tttcttaaca atcgacttgc taaagactct gctgttttct tactataacc
    43741 tgctgtaatt gctgcttttt taccattaca tccattcatt atatattcat ctgcgaatct
    43801 cttttgtttt tcgttcattt catttaccac caactctcgc gctatacgct ttttaaaatt
    43861 aaaaaaggat tggctataat cagccaaccc acatagaccc tttattccta attgcgataa
    43921 gggaaacgca gtacgatagt caatatccta cactatcata atatctcatt taaggtatca
    43981 aaaactgcca ctttactgcc aatttcagtc ttcccctaac tcttccgcca atctagatat
    44041 gatttttctt ttgattctat gagcagttct atcagaaatg tgtatgtcaa cacaaacttt
    44101 cactaattcc tttttattaa aataatactc ttgaatgaat tcgcgttctt tcctgcttga
    44161 tgtgttgatt atacgttcaa tagcgctctt aaactcaagg attttacctc ttcgtatact
    44221 acaaagataa ttagttactg ccatttctgt tttcgatgta ttagacggta caaactcccc
    44281 gcctatattt gtatctgttg gaatccatgg tgtcattatt tcacttctta aatcttcgag
    44341 ttgcttatga taattaggat aatcacacaa ctcatcttct aactttcgaa ctgttgataa
    44401 ttttaatcca tatttctttt tagtcatgaa taccctccat acaaatattt ttaatcttca
    44461 aaatgtctca atctacttct taatatctct atctcccgct ctttaacttt cacatcgcct
    44521 tttaactgtt cagcttgcaa catcacacca aacaataaga tgactagtaa tataattgct
    44581 atgactaacc acatcatcta ctctgacacc tccgccctca tcaaatcaga ctgatcgctc
    44641 aactttgcga agtcactcgg cgcctctaca tcatcattag ccgtcgtcat aatatatact
    44701 ttctcagtta catacttacc tagctcatac atcgctagta agaataatag tcttaatatt
    44761 tgtttaatca ttatttatct accttcttta cttcgtataa gaccggatat aaatttaaaa
    44821 agtgtattct ataaccaatc gttttaactt ctactttgtc gcctactttt aacctagctt
    44881 gtatgtctgc gctatcaaat ttctttttga atagtaagtc agagttttcg atgacttgct
    44941 tgttgtctaa cacaatatag aacttgtctt ctttatcttg tctcttgtta tatttatctg
    45001 taattgtccc ttgatgtact tctttgtttt ggtaactagc cactgtatag ataggcgata
    45061 tgacaacaag catcagtgcg attacgccga ataatcgcag tattccaaca ataaagatat
    45121 cgaaccaatc catattttta agttttttaa tcatcattgt catctcctgt atcaatcaaa
    45181 aaaagtacct gtctcaacat actctttaac tgttgttcat ttagactggc taacataggg
    45241 ctgtaaaatt cactatcttc atctttaaca gttttaataa aacagccttc aatctcagct
    45301 ttttcttctg gcgttccatt tttatacgtc ttaaatacct cggtgtgctt ttctggtaat
    45361 ttcattttag gtgtattaaa cattattatc tcccctcttt aatgatttta tttcttttcg
    45421 aacaaagaac ctaatacttc ttcactaggt ctttcgaata aggtcacttt agaattatta
    45481 gtgtagtaaa caataggtgt attttgtgac tcatatttct ctttcgcttc ttctttactc
    45541 tctgcctcaa caactgtaaa cctttgattg cttttagctc gagttatgtg tgtatgttta
    45601 cgtcctgttg aatctttgaa tgttgtgact aagtattgcg tcacttctta tcactccttt
    45661 gaatgattct aagtttttct acgaataaaa gtattagtaa aacactcaat gtagctaaca
    45721 tattttgttg ttttgcaaaa tctactataa cgattaagac taataacatt ccaattctgc
    45781 atgtaaataa atctaattct ttgtacaaaa ccatatatct gttgagtaaa ttgttaaata
    45841 ttactatgaa tacaagtatt agaactaatg taatgatgta actcacttcc ccaaaacctc
    45901 cttgactcga tctaagatgt ctttacactc cgctacttcc gaagcctttt tctccacgtt
    45961 ctgaaacact ttcgaattcc tccacttgct ttagttcagg tgtccatata ggcacgataa
    46021 ccaattgagc tagtttgtcg ccttcgttga tttgataagt tccatattgt cttatggcgt
    46081 cactcaaatc gatttctcct ttaatatcaa aaacacctgg tgtaatataa ccatttgatg
    46141 caatagcgtc attcttgatg ttaatcccta aattaccatg atatcccgcg tctatcttgc
    46201 ctgtttcaat cactaaatgc gttttactgc ttacaccact acggctagtt aatagtccga
    46261 catagccctc tggtatgctc acagctacat ctgttttaat cactgccttt tcttgtggct
    46321 c
//
LOCUS       DALAAR010000013        45954 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000013 DALAAR010000000
VERSION     DALAAR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45954
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.013"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(107..1075)
                     /locus_tag="O2458_000732"
     CDS             complement(107..1075)
                     /locus_tag="O2458_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCZ3886616.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            1270..1725
                     /locus_tag="O2458_000733"
     CDS             1270..1725
                     /locus_tag="O2458_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3886617.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            1965..3347
                     /locus_tag="O2458_000734"
     CDS             1965..3347
                     /locus_tag="O2458_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886618.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            3378..3506
                     /locus_tag="O2458_000735"
     CDS             3378..3506
                     /locus_tag="O2458_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886619.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            3616..4824
                     /locus_tag="O2458_000736"
     CDS             3616..4824
                     /locus_tag="O2458_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ3886620.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(5054..5605)
                     /locus_tag="O2458_000737"
     CDS             complement(5054..5605)
                     /locus_tag="O2458_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3886621.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            5726..6373
                     /locus_tag="O2458_000738"
     CDS             5726..6373
                     /locus_tag="O2458_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCZ3886622.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            6386..8317
                     /locus_tag="O2458_000739"
     CDS             6386..8317
                     /locus_tag="O2458_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCZ3886623.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(8563..9201)
                     /locus_tag="O2458_000740"
     CDS             complement(8563..9201)
                     /locus_tag="O2458_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886624.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            9409..10329
                     /locus_tag="O2458_000741"
     CDS             9409..10329
                     /locus_tag="O2458_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3886625.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(10437..10856)
                     /locus_tag="O2458_000742"
     CDS             complement(10437..10856)
                     /locus_tag="O2458_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886626.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(10853..11344)
                     /locus_tag="O2458_000743"
     CDS             complement(10853..11344)
                     /locus_tag="O2458_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCZ3886627.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            11441..12388
                     /gene="corA"
                     /locus_tag="O2458_000744"
     CDS             11441..12388
                     /gene="corA"
                     /locus_tag="O2458_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCZ3886628.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            12419..13468
                     /locus_tag="O2458_000745"
     CDS             12419..13468
                     /locus_tag="O2458_000745"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCZ3886629.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(13516..14724)
                     /gene="gltS"
                     /locus_tag="O2458_000746"
     CDS             complement(13516..14724)
                     /gene="gltS"
                     /locus_tag="O2458_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCZ3886630.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(15061..15669)
                     /locus_tag="O2458_000747"
     CDS             complement(15061..15669)
                     /locus_tag="O2458_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCZ3886631.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            16068..16715
                     /locus_tag="O2458_000748"
     CDS             16068..16715
                     /locus_tag="O2458_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCZ3886632.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            16946..17845
                     /locus_tag="O2458_000749"
     CDS             16946..17845
                     /locus_tag="O2458_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886633.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            17838..19070
                     /locus_tag="O2458_000750"
     CDS             17838..19070
                     /locus_tag="O2458_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3886634.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            19054..19239
                     /locus_tag="O2458_000751"
     CDS             19054..19239
                     /locus_tag="O2458_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886635.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            19288..19614
                     /locus_tag="O2458_000752"
     CDS             19288..19614
                     /locus_tag="O2458_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCZ3886636.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            19644..20663
                     /locus_tag="O2458_000753"
     CDS             19644..20663
                     /locus_tag="O2458_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCZ3886637.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(20899..21552)
                     /locus_tag="O2458_000754"
     CDS             complement(20899..21552)
                     /locus_tag="O2458_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCZ3886638.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            21726..22412
                     /locus_tag="O2458_000755"
     CDS             21726..22412
                     /locus_tag="O2458_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCZ3886639.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(22478..23737)
                     /gene="sdpC"
                     /locus_tag="O2458_000756"
     CDS             complement(22478..23737)
                     /gene="sdpC"
                     /locus_tag="O2458_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCZ3886640.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            24027..24962
                     /locus_tag="O2458_000757"
     CDS             24027..24962
                     /locus_tag="O2458_000757"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCZ3886641.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(25218..25637)
                     /locus_tag="O2458_000758"
     CDS             complement(25218..25637)
                     /locus_tag="O2458_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ3886642.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(25759..26643)
                     /locus_tag="O2458_000759"
     CDS             complement(25759..26643)
                     /locus_tag="O2458_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3886643.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            26803..28464
                     /gene="hutU"
                     /locus_tag="O2458_000760"
     CDS             26803..28464
                     /gene="hutU"
                     /locus_tag="O2458_000760"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCZ3886644.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            28464..29702
                     /locus_tag="O2458_000761"
     CDS             28464..29702
                     /locus_tag="O2458_000761"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCZ3886645.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            29979..31100
                     /locus_tag="O2458_000762"
     CDS             29979..31100
                     /locus_tag="O2458_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCZ3886646.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            31211..32092
                     /locus_tag="O2458_000763"
     CDS             31211..32092
                     /locus_tag="O2458_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ3886647.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            32579..33148
                     /locus_tag="O2458_000764"
     CDS             32579..33148
                     /locus_tag="O2458_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886648.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      33201..33296
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            33373..34686
                     /locus_tag="O2458_000765"
     CDS             33373..34686
                     /locus_tag="O2458_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCZ3886649.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            34853..35359
                     /locus_tag="O2458_000766"
     CDS             34853..35359
                     /locus_tag="O2458_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ3886650.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(35417..36181)
                     /locus_tag="O2458_000767"
     CDS             complement(35417..36181)
                     /locus_tag="O2458_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ3886651.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            36511..38115
                     /locus_tag="O2458_000768"
     CDS             36511..38115
                     /locus_tag="O2458_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ3886652.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(38181..38711)
                     /locus_tag="O2458_000769"
     CDS             complement(38181..38711)
                     /locus_tag="O2458_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886653.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            38979..39896
                     /locus_tag="O2458_000770"
     CDS             38979..39896
                     /locus_tag="O2458_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCZ3886654.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            39971..40606
                     /locus_tag="O2458_000771"
     CDS             39971..40606
                     /locus_tag="O2458_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3886655.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            40617..40808
                     /locus_tag="O2458_000772"
     CDS             40617..40808
                     /locus_tag="O2458_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886656.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(40904..41089)
                     /locus_tag="O2458_000773"
     CDS             complement(40904..41089)
                     /locus_tag="O2458_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886657.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(41086..41442)
                     /locus_tag="O2458_000774"
     CDS             complement(41086..41442)
                     /locus_tag="O2458_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886658.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            41722..43101
                     /locus_tag="O2458_000775"
     CDS             41722..43101
                     /locus_tag="O2458_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3886659.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(43488..44360)
                     /locus_tag="O2458_000776"
     CDS             complement(43488..44360)
                     /locus_tag="O2458_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ3886660.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(44805..45134)
                     /locus_tag="O2458_000777"
     CDS             complement(44805..45134)
                     /locus_tag="O2458_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886661.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            45183..45920
                     /gene="sdpB"
                     /locus_tag="O2458_000778"
     CDS             45183..45920
                     /gene="sdpB"
                     /locus_tag="O2458_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCZ3886662.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT        15742 a         6379 c         8509 g        15324 t
ORIGIN      
        1 tttatttatt ttcaatataa caattgtcta atcaaggatt aacgaatatt taaagatagt
       61 ttgacgcaat attagaaaca acctataata atagtttgtt tgtggattaa ctattataaa
      121 taaaagcggc gtaaagacat ataaaccaac tacttgaaca atataacgtt aataacaatc
      181 tatactgata cattacgcct agataatctt tgatgagcac atgtaagaaa aagtgatatg
      241 gtgtatgact tccgacacca tcgatagata aacctaattt ttgggctagt cgtaaggcgc
      301 gcaatacatg aaactgactt gttacacaaa caattttaac tgcttcatga tacaaattgt
      361 tgatgatttg tttagaatat aaaaagtttg tgtatgtatt tatagagtga gattccatta
      421 gtatatctgt tttatcaaca ccatgtgcaa tcaaataacg ttgcatagct aaagcttcag
      481 aaattggttc gtctggtcct tgtccgccag atacaatgat ctttgttgct gatgcttgtt
      541 gttgatagat atcaagtgca cgatctaaac gcgctgcaag cattggtgtg acaaattcgg
      601 taaaaatacc agcacctaac acaattatga tatcaacttc tttgttgtat gatctatgtc
      661 tatatgatac tgtccaaacg agataacaaa taaaggttag taacagggaa agacataata
      721 tagctaacca catagacaaa cctttcacaa taggtgactg aatcgtactt ataaatagaa
      781 gtgctgatgt gtagagtaca aatttatatg aaaaagataa taatttttta ataaataagc
      841 gactagaagt atgagaaaat aaatatctat gtttgaatag catgataata ctgattatta
      901 taaatgttac aaacatagac caagggaaag tataggtcat gatgctatag atgagtgaca
      961 aaaatatcga tatgacaact aagatgtagc atgttaaatt taacgtcaga gtatagttga
     1021 aaattaacgg acaaataacg ataagtataa atattaataa taaattcaat aacatactga
     1081 cacctcgctt ataataaata ttaaatataa atgtagatga tttaatttat taaagcaagg
     1141 agaaagcagc aacatgtaaa tcttaatttg ttatattata tatgggtcaa tatttttgtg
     1201 ttttttagat gtgcgataca atgattgctg aggatacttt ataaaaatag taattaggga
     1261 gaaataatta tggtaaaaca tttacaagac catattcaat ttttagagca gtttataaat
     1321 aacgttaacg cattaactgc aaaaatgttg aaagatttac aaaatgaata tgaaatttca
     1381 ttagagcagt ctaacgtatt aggtatgtta aataaagaac ctttgacaat tagtgaaatc
     1441 acgcaaagac aaggtgtaaa taaggccgca gtaagccgac gaattaaaaa gttaatcgat
     1501 gctaaattag ttaagttaga taaaccaaat ttaaatattg atcaacgttt gaaattcata
     1561 accttaactg acaaaggtag agcatatttg aaagaacgta atgcgattat gacagatatt
     1621 gcgcaagata ttactaatga tttaaattct gaagatattg aaaatgtaag gcaagtatta
     1681 gaagtcatca atcatcgcat taaaacatat agcaatcata aataggtttt tgaaggtgtt
     1741 caaatataag actaaatatg tatgtattga acaccttctt tttagttata attgagagag
     1801 tactttcata aataatgtaa taaggtacaa ttctaaagta aagttaatca tttcttgaat
     1861 tgtattcttg ggataatatt tgtaaaataa taatggtaaa ataatgaaat aatttttgtg
     1921 atatacatat aacaattgaa gaggaacaga attgaggtga aagtatgaaa tcttgcccga
     1981 agtgcggaca acaagcacaa gatgatgtac agatatgtac acaatgtgga cataaatttg
     2041 atagtcgtca agcattatat agaaagtcaa ccgatgaaga tatacaaact aataacatca
     2101 agatgagaaa aatggtgcct tgggcaattg gttttttcat tttaatctta attattatat
     2161 tgttcttttt actaagaaac ttcaattcac ctgaagcgca aactaaaata ttagtgaatg
     2221 cgattgagaa taatgataaa caaaaagtag caacattatt aagtactaaa gataataaag
     2281 tagattctga agaagcaaaa gtatacatta actatatcaa agatgaagtt gggcttaagc
     2341 aatttgtcag cgaccttaaa aatacggtac ataaattgaa taagagtaag accagcgtag
     2401 cttcttatat tcaaaccaga tctggtcaaa atatattacg tgtaagtaaa aatggcacac
     2461 gttatatctt tttcgataat atgagcttta ctgcacctac caagcaacca attgttaaac
     2521 cgaaagaaaa aacaaaatat gagtttaaat ctggtggtaa gaaaaagatg gttatagctg
     2581 aagcaaataa agtgacgcca ataggtaatt ttataccggg gacatataga attccagcta
     2641 tgaaatcaac tgagaacggt gattttgcag gccatttaaa atttgatttt agacaaagta
     2701 attctgaaac ggtagatgtt actgaagatt ttgaagaagc aaatatatct gtaactttaa
     2761 aaggcgatac aaaattaaat gatagttcta aaaaagtaac tataaatgac catgaaatgg
     2821 cattttcaag ttccaaaacg tatggtccat atccacaaaa taaagatatt accatttcag
     2881 cttcaggtaa agcgaaagat aaaacattta caacacagac gaagacgatt aaagctagcg
     2941 atttaaaata caatacagag ataactttga attttgacag tgaagatatc gaagactatg
     3001 ttgaaaagaa agaaaaagaa gaaaacagct tgaagaacaa attgatagaa ttctttgctg
     3061 gatattcttt agcgaataat gctgcgttta atcagtcgga ttttgatttt gtatcatcat
     3121 atataaaaaa aggatcatct ttttatgatg atgtaaagaa gcgtgtatct aaaggaagtt
     3181 taatgatgat tagttcacca caaattatag atgctgaaaa acatggtgat aagattaccg
     3241 caaccgtaag attaataaat gaaaacggta agcaagtaga taaagaatat gagcttgaac
     3301 aaggctcgca agaccgcttg caattaatca agacatcaga aaaatagagt ataaaactta
     3361 aataagatgc atgtgtcatg gaatacaaac aacatccttt caaagtattt acaattagtg
     3421 tgaattactt tgaagggatt gttttttgtg gaaaaattta tgaagtgctg aggtttatat
     3481 cattttatac cttttataag ttgtaatgat gttcatgaaa cttgtgacta atgctaatat
     3541 ataaaagagc atatttaaat ttagtttctg ttcgtgatag ttataaacta atcattatat
     3601 aaaggagata gtcatatgaa tgatttaacg cattttaaca agcgttcacc gatatttatt
     3661 attattttag gatctttgac ggccattgga gcattatcga tagatatgtt tttgcctgga
     3721 ttaccagata ttagacatga ttttcaaaca acgacgtcta acgcacaatt aacattatcg
     3781 atgtttatga ttggtttagc gtttggtaat ttgtttgctg gtccaatttc agattcaact
     3841 gggcgcagaa agccgcttat tatagctatg attattttta cattagcaag tttaggtatt
     3901 gtttttgtac ataatatttg gctgatggtt gcattaagat ttcttcaagg agtgacaggt
     3961 ggcgcagcag cagttatttc aagagctata gcaagtgata tgtatagtgg taatgaacta
     4021 actaagttta tggcattatt gatgcttgtt aatggtattg caccagttgt tgcgccaaca
     4081 atagggggca taattttaaa ttattctgtc tggcgtatgg tctttgtcat tctgactatc
     4141 tttggcttcg tcatggtcat aggttcttta ttaaaagtac ctgaatcatt aacagtgaca
     4201 aatcgtgagt caagtagtgg attaaagaca atgtttaaaa acttcaaaat attattgaag
     4261 acgccacgtt ttgtattacc aatgctcatt caaggtatga catttgtaat attatttact
     4321 tatatttctg cgtctccgtt tattattcaa aaaatttatg gcatgactgc aatacaattt
     4381 agttggatgt ttgcaggaat tggtattacg ctcattatat caagtcaact tactgggtac
     4441 cttgttgatt ttatagactc acaaaaattg atgagaggca tgacgatgat tcagattatc
     4501 ggtgtaattt tagtgacgat tgtattgctt aatcattgga acttttggat tttagcgata
     4561 ggatttatca ttttaatagc accagtgaca ggtgttgcga cacttggttt tacaatagct
     4621 atggatgaaa gtagtagtgg tagagggagt tcatcaagtt tattaggttt agtacagttt
     4681 ttatttggtg gtgttgcatc gccattagtg ggggtaaaag gtgaagataa tcctatacct
     4741 tacataatta ttattatagc tacagcagtt atattaatta ttttacagat ttataatatg
     4801 aaggtattta aaacaaatcg atgataaaca tagtttcttc tattatattg aattgaaaaa
     4861 ttaaaaaaca aaacttgagc tattcataga cctatgaatt tagcatcaaa aataaaaatg
     4921 ccgagattat tatgtggaaa tctcggcatt tttatattga tagcaccgtt acatatgtac
     4981 tattatctat ccatttggtt taactttatc tagttgtttt atagcatttc tttattagaa
     5041 tgaaaccgta ttattagttt tgaagtttaa tcgtattagc caataaaatg tcagcaactt
     5101 cttcaggtga ttcattacaa ccattcgaaa gccaattaat aaacacccca gcttgaccgc
     5161 ctatgatata tgtaacataa agctgtttat tggttactgt agtactatag tcattcataa
     5221 tttgttgata gtaatctctc gctaaattta tatagtctat gaataatgtt ttcattggat
     5281 aggtaactaa aatgtctttg aaaaactgct cattgtgctt gatatactta gcaataataa
     5341 tgaagaattt ataaagttta agcttatcct gtttaatatt ttcgaaatta tgatgtagcg
     5401 cgcttagaag aaattggaat ttttccttat gctttgagtt gatggtatcc aataaatcaa
     5461 atttatcttt gtaataatca taaaaagtag aacgattaat attacaatac gcgcaaatca
     5521 ttttagtgga tatttcttga aaaggatatt cagccatcaa atcaacaagt gcgttttgaa
     5581 tttctatttt ggcttgtcgt ttcatattgt tacacctttg tgaattttaa ccaacactct
     5641 tataaaaatt gttggtgttt ttttagttat catgtctcta taataaataa agtgaatttt
     5701 aaaagcaaat aggaggaaat gtttcatgaa aaagatgata ttaattaatg tcattacggt
     5761 tgttgtactg ttagcaattg ggattgctgg cttttatttt tggaataaaa caacaagcta
     5821 tgtaacaact gataatgcaa aagtaaatgg cgatcaaatt aaaatagcaa gtcctgcatc
     5881 tggacaaatt aaatcactta atgttaaaca aggtgacaaa ctcgataaag gtgacaaagt
     5941 agcaattgtt actgtacaag gtcaagatgg cgaaacgaaa gatatggatc ttaaaatgcc
     6001 tcaaaaaggt acaattgcca aattagatgg tatggaaggt tcaatggtgc aagctggtaa
     6061 cccaatcgct tatgcataca atttagatga tttatatgta acagcaaata ttgacgaaaa
     6121 agatattaaa gatgttgaag taggtaaaga tgttgatgtg actattgacg gtcaaaaagc
     6181 ttctattaaa ggtaaagttg atagtattgg aaaagcaact gctgcaagct tttcattaat
     6241 gccatcatca aatagtgatg gtaactacac taaagtttct caagtaatac ctgttaaaat
     6301 cactctagaa tcagaaccgt ctaaacaagt tgttcctgga atgaacgctg aagtaaaaat
     6361 ccataaaaat taagggggtc attaaatgac tacgaccttc attattagct acattatttt
     6421 agcgctcatt attgttgggg ttatcaattt atttttaata agatcaagaa aaaaaggcaa
     6481 acgccaacaa aaggaacaac aatttacgac acgtcaatca aatcaatcta aatttaaagc
     6541 tagtgattta gacaaaacaa ctgatcaatc gacacaacgt atgacgcatg aagagttgcg
     6601 tgttgacaat caagatgatc atagccaagt tagtctaaat ggttacacaa aggggtctga
     6661 gaaagatcaa gaagcattca ctaataataa ggatgaggaa gcagttgctg ctaaaaatcc
     6721 tgaatcagaa gaatataaag tgaatgaaaa aataaaaaaa gaacataaaa actttatttt
     6781 tggtgaaggt gtttcacgcg gtaaaatatt agcggcatta ttattcggta tgtttattgc
     6841 gattttaaac caaacattat taaatgttgc tttaccaaaa attaatacgg aatttaatat
     6901 ttctgcgtca acagggcaat ggttgatgac gggattcatg ttagtaaacg gtattttaat
     6961 accaattacg gcgtatctat ttaataaata ttcatatcga aaattatttt tagttgcgtt
     7021 agtactcttt acaattggtt cattaatctg tgcgatttca atgaatttcc caatcatgat
     7081 ggttggacgt gtattacaag ccattggtgc aggtgtacta atgccattag gttcaattgt
     7141 tattattaca atttatccac ctgaaaaacg tggtgctgcc atgggtacaa tgggtatcgc
     7201 aatgatttta gcacctgcaa ttggtccaac attatcaggt tatattgtac aaaattacca
     7261 ttggaatgtg atgttttacg gtatgttcat tatcgggatt atcgctattt taattggttt
     7321 tgtatggttt aagttatatc aatatacaac aaaccctaaa gctgatatcc caggtatcat
     7381 ttttagtacg attggttttg gtgctttgtt atatggtttc tcagaagctg gcaacaaagg
     7441 ttggggttca gtagagatag aaacaatgtt tgcgattggt attatcttta ttattctatt
     7501 cgttattaga gaattaagaa tgaaatcacc aatgttgaat ttagaagtat tgaaattccc
     7561 aacatttaca ttaacaacaa ttattaatat ggttgtaatg ttaagtttat atggtggtat
     7621 gattttatta ccgatttatt tacaaaattt acgcggattc tcagcattag attccggatt
     7681 gttattatta cctggttctc taattatggg tctactagga ccatttgcag gtaaattgtt
     7741 agatactatt ggtttaaaac cattagctat ctttggtatt gctgtaatga cttatgcaac
     7801 atgggaatta actaaattaa atatggatac accatatatg acaatcatgg gtatctatgt
     7861 acttcgttca tttggtatgg catttataat gatgccaatg gtaactgcag ctattaatgc
     7921 gttaccggga cgacttgcct ctcatggtaa tgctttctta aatacgatgc gtcaattagc
     7981 aggctctata ggtacagcaa tcttagttac tgtaatgaca acacaaacta cacaacactt
     8041 atcagctttt ggggaagagt tagataaaac gaatcctgtt gtacaagatc atatgcatga
     8101 attagcatca caatatggcg gacaagaagg cgcaatgaaa gtgttactac aatttgtaaa
     8161 taaactagca acggttgaag gtattaatga tgcatttata gttgcaacga tatttagcat
     8221 catcgcctta attttatgtt tatttttaca aagtaataaa aaagcaaaag ctacagctca
     8281 aaagttagat gcagataata gtatcaatca tgaataaata aaataaatta attgaagtgt
     8341 gactaatcaa aaattatgtt gtggggacat gatttttaaa gtatcggtgc caaatatggt
     8401 tatcgatact ttttttattt gttgatttat agaatgttag aggaattata ttaaaatttg
     8461 gcattgacgt agtaggtcat taataaagaa aaagcaggaa gtgggtcaac gaaatgaatt
     8521 ttgtgaaaat aacatttctg tcccaatccc tactatataa cattatttta aacgaggcat
     8581 gcgattacgg aagaataagc ttataacaag taaaccgatg ctacagccaa gtaaaatgat
     8641 gccgttatga atagcgtcag cttctgtaat cacttgatct ggtggtacat ttaaataata
     8701 ttttttgaaa acatctgcaa tttcttgtgc gagtgcttta ataatataac tgaaatacaa
     8761 tgtaatgact aacataattg ttaacactaa aatatttact acttttagaa ctgtgcgatt
     8821 aaatacaatt gtcagtattg ttatgactag gctaacgata cataaaataa agaaaccgtt
     8881 aaagactaaa attaagcgat cgattccttg ttgtaaatca ttgatattac ccaaattaat
     8941 atttaattga tgcatttttc caattgtctc gttaaaattt ttaatgccat caaaatctac
     9001 agattctttg gtgaagacaa tattaaaaat gggttgtttc cacatgctgt atccagcgat
     9061 tgcggttaat ataacaacaa tgatttgaat aatcatgctt accaatgaac gttttttctt
     9121 accatatcgc ttaccacgat gaatattcgg ttgttgaaat gtctcatcaa atggttctaa
     9181 tcgttgattt ggatttgtca tgcaaggtac ctccgtataa agttcttttc tttacttatc
     9241 aatatgtcta gttgtctgtt ataatgatga aaaatcactg ttcaattgta gtcttaaatt
     9301 tgaaagtgaa aaagaaaata tcttctacat tatatacttt ttttggcaaa gaatgtaggg
     9361 gatagacaat cattggatac ataaaatata taaaggtagg ttaagacatt gattagaaac
     9421 cgtgttatga atagcgttgt caataaatat ttgcttcaca atcgctcgat tatgtttaaa
     9481 aatgatcaag atgttgaaag atttttttat aaaagagaaa ttgaaaatag aaaaaaacat
     9541 aagcagcctt caacattaaa tgttaaagca aatttagaaa aattgtcatt agatgatatg
     9601 caagtctttc gctttaattt cagacatcaa attgataaga aaattttata tattcacggt
     9661 ggattcaatg cactacaacc atcaccgttc cattggagat tgttggataa aatcacttta
     9721 agtacattat atgaggttgt actgcctatc tatccaaaga caccagagtt tcatattgac
     9781 gatactttcc aagcgataca acgtgtttat gatcaattag tatctgaagt aggacatcaa
     9841 aatgtcgtag tcatgggtga tggttcaggt ggtgcactgg cattatcctt tgtacaatct
     9901 cttttagata atcaacagcc attaccgaat aaattgtatt taatctcacc aattttagat
     9961 gcaacactat ctaataaaga tatttcggac gccttaatcg aacaagatgc ggttctaagt
    10021 cagtttggtg tcaatgagat tatgaaaaaa tgggcgaatg gcctaccatt aacagataag
    10081 cgcatatcgc caattaatgg cacaatagaa ggattgccac cagtttatat gtttggtggt
    10141 ggacgtgaaa tgacacatcc tgatatgaag ctatttgaac aaatgatgtt gcaacatcat
    10201 caatacattg agttttatga ttatcctaag atggtacatg attttccaat ttatccaatt
    10261 agacaatcac ataaagctat taaacaaatt gccaaatcga tagatgagga tgtaacacaa
    10321 aataactaat aacatataaa tgacccagtt acgatttcat gcaagatgat atttgacatg
    10381 aaaacgtaat tgggttttgt ttttgaaaat atagcaaggg ttatatagac taattgttaa
    10441 tgagcttctg agtgaaaatc accaataagt gtattgatat gtgtgatgat aaagtattgc
    10501 cctttaattt gataaaattg atctaaaaaa ttatcgaatt gacgtggaat atcactaagt
    10561 tctgcaaagt tagctgtttt atcataattg cgatgagcaa cctcattttg aaatttatca
    10621 taatgttcta ggaaatgttg atcttcaagc tgttgttcca caatagtcat aatattatcg
    10681 atggcttgct tttgtccttg aacataagcc atatgcacgc cacgcttaag tagctgattt
    10741 gctggtccgt taatgtataa ccctttttgt gcatttaatt ctgttaattc aaacttaaca
    10801 tcatctaata aatctagtaa ttcgatatga atcttttgtt gtaatgcatc tgtcatttta
    10861 atgctccttt gtgtggaatt gctattttat gtcctaattg ttcaggccaa gtgatagttg
    10921 attgattttt ataatagtgt gctatttgtg ttgaaaatga ttcaggaaaa gcatcagaac
    10981 gacgtgtgtg ctgattaatt cccatcatca ttacttcatt tgttgatgct agtgtaccat
    11041 cttctttagt taatgttaaa aataaatgca accgcttata atcgtaatca taaatatata
    11101 aagtaacagt aaatacatcg ccaagagaca attctgagag gtatgtcgta tgttcttcta
    11161 gtgtaaatag cgtatatgct aaattttcgc gttcttttaa agaaagaccg tggctgtaat
    11221 taaaacgatt cacgacgtca ctaaaaatga tattataatt tgcatcatgc atatgattgt
    11281 tatggtcaat gaattcagat gttacggttt gagtatgtgt aaaaagttgt ttgataataa
    11341 ccatctcctt agtgactatc atactaaaat aaattaagac atacacgtta tttaattcat
    11401 gataaaataa tagagaattc gttatttagg gggagtatcg atggctttaa agatacggta
    11461 tcaaactaca tatgaaccgt ttaaagtagt tgatgatatc aaagagatac caaaagatgc
    11521 gactattgtt tggtatgatt ttgatgaacc gaatgaacaa gaaaatgaat ggtttaaagc
    11581 acattttaat tttaatgatt tagaagtgga tgatgcaatt aacggtatgc cacgagctaa
    11641 atataaatct tataaagatt atcaatactt agtattccat agtattatgg ggtcaaacta
    11701 ttcaccaata gcactaaata tttttataca agacaatgta ttagtaacat atcatcatca
    11761 aacgttggaa tcattaaata aagtagtcta taagtatatg aatacattag atgcagaatt
    11821 agattgtgca gatgtcgtca tattaatttt agatatgatg gttgataaat atttcaactt
    11881 tgtatatgca cttgaagata gtgtttatca ttttgaagac cgccatgtag atgatcgctt
    11941 taataaaatg gtcatggata gtgtctttaa gttacgttcg gatttgatta aagtaaaacg
    12001 tgtactgttt cctatgcaag agctaattga tacaatgaaa caaaatggtg atttgattat
    12061 agataataaa cattcgctat acattcaaca tatcgatgac cacttgatta agcaacgtaa
    12121 tatcattcgt actgctcaag aaatgacaaa cgaaatacga gaaaattttg agtcatacac
    12181 gtcatttaga atgaatagta ttatgcaagt actgacatta gtatcagtta tattttcacc
    12241 gttaacattc atagctggga tttatggtat gaactttgta aatatgccgg cattacattt
    12301 acattatgga tattatattt gcttagctgt aatgtttgtt atagctgtcg tattaatcat
    12361 ctttttcaga cgtaaaaaat ggttttaaat tagaaatgac gaaaaggtag gtttaatcat
    12421 gagtgatttt caaagagaac agagaaaaaa tgaacatgtt gaaatagcaa tggcccaatc
    12481 tgacgcaatg cattcagatt ttgataagat gcgttttgtg catcattcta tcccatcaat
    12541 taatgtaaat gatatcgatt tgacatcaca gacgcctgac ttaacgatgg catatccggt
    12601 ttatattaat gcaatgacgg gtggtagcga gtggacgaaa aacatcaatg aaaagctagc
    12661 tgtagttgca agagaaactg gcttagcgat ggcagttgga tcaacacatg cggcattgag
    12721 aaatccacgc atggctgaga cgtttacgat tgcgcgaaaa atgaatcctg aaggcatgat
    12781 ttttagcaat gttggtgcgg acgtaccagt agaaaaggct ttggaagcag ttgaattact
    12841 tgaggcacaa gcgttacaaa tccatgttaa ttctcctcaa gaattagtta tgcctgaagg
    12901 gaatcgtgaa tttgtgactt ggttagataa tatagcgtcg attgtatcac gagtgtctgt
    12961 tccagtcatt ataaaagaag ttggatttgg tatgagcaaa gaattaatgc atgacttaca
    13021 acaaataggc gtcaagtatg tcgatgttag tggcaaaggt ggtactaact ttgtagatat
    13081 tgaaaatgaa cgtcgtgcaa ataaagatat ggattactta tcatcatggg gacagtctac
    13141 agttgagtca ttacttgaaa caacggctta tcaaagcgaa atttcagttt tcgcgagtgg
    13201 tggtttacgt acaccactcg atgcaattaa aagtttagca cttggtgcaa aggcaactgg
    13261 tatgtcacgt ccgtttttaa atcaagttga aaataatggc attgcacata cagtagctta
    13321 tgtagaatca tttattgaac acatgaaatc aataatgacg atgttagatg ctaaaaatat
    13381 tgacgactta acacaaaaac aaatcgtatt tagtccggaa atattgtcat ggatagaaca
    13441 acgtaacttg aatatacatc gaggataaat aaaagaggag gcgaaggcct cctcttttat
    13501 ttatgagttt ggtgtttaac taaaccattg tatgaatccc ataatgacta taacaccaat
    13561 taaatcgatt aagaatgcac caacaatagg tacaactaaa tatgctttag gtgagtttcc
    13621 atatttttta gtaataacat ctaaatttgc catggcattt ggcgttgcac caagcccatg
    13681 accgataaaa ccacctacca ttactgcagc atcataatct tttcctaaac ctctaaataa
    13741 aattaaaaca gcaaataaaa tcataacgac aacttgaact aaaacgataa taataagagg
    13801 tatagcaagt ttataaattt cgattaattg aatgctcatt agcgcaagag ataagaaaat
    13861 acctaatgcg acatcgccaa tttgattagt aatttttaaa tctacaatat taaaattaaa
    13921 actttcagag atatttcgga caataacagc tacaaataat gagccaacgt aaattggaac
    13981 attaatccct gtttgagctg taaacaaatg actgaaataa cttccaactg ccatacagaa
    14041 tacaacgatt gtaaattgaa tgaagaatac ttcagtggca ttaaatttac tatgtaaatg
    14101 ttcgttatat gctacttggc tataatcttt aaatgtatcg tcactatgtt gaggctttaa
    14161 gttataacgt ttgattaaaa attttacaac tggaccaccg ataagccctc caaatacaag
    14221 acctaaagtt gcagctgcaa gagccgctgt cagtgccgaa tcaataccta aatcttgaat
    14281 tgtcttacca taagcagcag cattaccatg accgccttcc atagacatgg aacctgctgt
    14341 taatcctaac aaaggtttaa tatttaatac ttttgctagt gatacaccaa ctatgttttg
    14401 aatgactgaa atgatagcac aaaacataaa gtataatagc aagactttgc cacctaattt
    14461 aaataatttc aatgatgcac caagaccgat tgtcgtaaag aatgctaaca tgaagaaatc
    14521 ttgaatgaat gaagcatcta atttaatctt aaccatgcca aatgaatcca aagccgcaac
    14581 taaaatagca aagattaagc cgccaatcac tggtgctggt atacaaatac gttttaaaaa
    14641 attaacgtga ttaacgatag ccttaccaag taaataaagg atacaagcta aacataatgt
    14701 tgtaattgca ttaagttcta tcattttcaa atcccccttg ttgaatatcc ctctccctaa
    14761 ataagctttt atatgtaagc gttataaaag ggcgctttta acattataca tgcaacaaat
    14821 caatatttct agtttttgaa aataatatca ataatattaa tgcatctaaa tttaataatt
    14881 ataaataatt ctattgttat attttataat taagaacaag ttcaataaga ggagatgaat
    14941 caaaaaatat gatggataca aagaaaaaca atcaacccat ccgcatgttg acggcacgat
    15001 tgattgactt ataaggagat agatttcatt ttcatgatag taatcaatga aagatggcat
    15061 ttatttccaa gtatcttcgg gaaacataca atctgattta cgcatgcgag acacaaatgg
    15121 attacctttc actgtgtaac gtaaagattt atgtgtccaa tcacctttat ttggaatacc
    15181 gattcgtgga ctagcaataa tatctttagg atatttacga ttcttagtat caatagacaa
    15241 tctacagtca tttaacgtag cgccatcgat agcccgtgga atgttaaatg ccttagtcca
    15301 ttttcctggg ccattcgtta cctcgtagcc tttcttacct ctgttacgga acatagcgga
    15361 taaaccttct tctggttcaa ttgcgcggat aagtacgcct tcaggtatac cttcagattt
    15421 tgttacaaaa ttaatgagta aatgcgtatg catgacatgt gcataaattg taccaccacg
    15481 tttatataat gacgtgactt taggtgttat tttaccgcca tagccatgag ccgcacgatc
    15541 attcaaacct aagtaagctt ccgtttccac gatgtagcct gtatacgttt gagtggtatc
    15601 ctgataaatc actttgacac ctaataagtt tttagcaatt tgtcttgtat cattattaac
    15661 gaaatccata atattctcct cgcaattata tgattaattt aaataaaaat gaaaaagata
    15721 tttttattgt ggtaaatgtt tgataaaata gtattgttaa gaattataaa aatgtaaagt
    15781 atatactttg ttatggaata ttttatttgc atagattgag actcttttaa acgtgaacat
    15841 tgcagtatga catattttta gtatagttaa cgaagcgttg actttcaaat gagtctccat
    15901 atagatgtgt acttgtagcg ggttttcatt ttactagtga tgaaatataa agttcatttt
    15961 ccaaagtgat tgacgattga aatgaagtgg cttgataatg aatcttgtaa attataacgg
    16021 taacaataat taaaaatata aatatgatta acgaaaggga gattacaatg gaacaattac
    16081 ctcaagtagg ttttatgaaa tcattcgttt tattttggaa aaattatgta aattttaagg
    16141 ggagatcaag acgtagtgaa tattggtata tggcactatg gcatttgata cttatggttc
    16201 cagctgtgtg tgtgctaata ataaacctat ttctattctt tatttctata gcggcggata
    16261 acgaaacatt aatagttatg agtatattga tgattatcct tacatctgtg tatacttttg
    16321 tctatagttt ggcaacgttt attccaaatt tagcgttgtg cgttcgaaga tttcacgata
    16381 tatcaagaac aatgttattt ccaatgatta tgacagtatt cagtattata ttttatatag
    16441 tattacagat tatcgatagt tattatgaca gtgattttac attaatgccg atgggtctta
    16501 atattttgtt agtacttctt tattttgttt atttaggcct aacaattgtt atgatagtat
    16561 tcctatgctt tgacagtaag ccggcaaata aatatggcga aagcccaaaa tacccgtcta
    16621 caattaaaaa tcaaccagtg acatctgaaa caccggaaaa agcgtgtaca tctgaaacac
    16681 cgaaaacacc ggaagaaaca gataatcaat tgtaatgata taaatatctc ggcacaatga
    16741 tgctgggata tttttttaca aataaggcaa taaaggcatg gaaaaaacat atctagtgct
    16801 aatgatactt aacatgcaaa aatggcacta tttaaatttt tcaatttcag aaattttaaa
    16861 aataaattca ttttaaaaat attgcattta tagagaatgg aaatgttata ttaagataaa
    16921 tacatatttt tttaggaggc aaagtatggg acttgtaatt gaacatgtta cgaaacgttt
    16981 tggcaagatg acggcagtaa atgatatttc attaaagtta gaatcaggta aaatgttagg
    17041 ttttctagga agaaatggtg caggtaaaac aacgacattt cgtatgattt taggcttaag
    17101 tgaaccaact gaaggacaca ttacgtataa cggtaaaaaa ctagataaaa caatgtacaa
    17161 tcgtattggt tatttaccgg aagaacgcgg tttacatggg aaattgacag ttgaagaaga
    17221 attgaaatat ttggcaactt taaagggaat gtcaaaaaca gaaatacaac agcaaatatc
    17281 gtattggctt gagcgttttg atattactga aaaccgcaaa aaacgaattg atagtctatc
    17341 aaaagggaat caacaaaaaa ttcagctgtt agcaagtatg ttacataaac cagaattact
    17401 aatattagat gagcctttca gtggtctaga cccagttaat gttgagttat taaaagaagc
    17461 agttaaagac ttaaatgatt ggggcagtac aatcgtttat agttctcacc gtatggagca
    17521 tgttgaagaa ttatgtgatg atgtttgtat tttagataaa ggtcaacttg ttgtttctgg
    17581 tgatatcaat catgtacgtg catctaatgg taacaagaaa gttgtcattg aatcagaaac
    17641 aacactacca gatttaacaa acattagagg catcattcat agtgaaaaca tgaagcaagg
    17701 tcttcagttg acgattgaga atgaagacgt agccaaagat atttatcaag ttgtggcaca
    17761 tcaaggttat gttaaacgat tccaagttgt agaaccatca ttacaagata tattcattga
    17821 gaaggttggt ggcaaggatg cataagtttt gggcgacatt tactttgaca tatatcaaaa
    17881 aaattaaagc taaatcattt gtaattttta tgattattat tgctgcctta atgattggcc
    17941 tatctaacat tgataagatt attaacatgt ttgatgatgg tccagataaa attggtgtag
    18001 cagcaccgaa tgaacaaatc tataaggttt tcaagcaaca ggcgaataca tttcatagtg
    18061 atgctaagtt tactaaagtt tctatagaag atgctgaaaa agaagtaaaa aaacataaac
    18121 ttgataaagc ttatattatc aaggtgaatc aaaatcgcac gttacaaggg acaatcatta
    18181 gtgagaagcg agtaagtcat gaagattcac aaaaagtaca agcactgctt acagctattc
    18241 aaacaaatat ggtagccggg gaattaaata tcaacaaaga agacttacaa aaattacaag
    18301 cacaaagtaa agttgataat aaggtgattt ctaatgatga agtcgataaa gtatcagaag
    18361 gacaaaagat tttcaactat gctttagctt atggcattat ctttctaatg ttcttcattg
    18421 ttttaaatta tgcatctcaa atagctatgg aaattgctag tgagaaaaca tcgcgtgtca
    18481 ttgaaatgat tattacaagt atttcaccaa tccaacatat ttttgctaaa attttaggcg
    18541 ttatagcagt tgccgtgact cagattattt taattgtatt gatggcagtt atatgcattt
    18601 ttgcctttga cttaaaagac ttgctacaag gttttaacgt ggaaatgaat caattgtctt
    18661 ggcaaattat tattgtcgga attataagta gtattgttgg aattacggcg tatgtattac
    18721 ttgcagccat cctcggatca ttaacatcta gaatagaaga tttaaatcaa tctttgatgc
    18781 cactgacatt acttggtatg attgcatttt atatcgcgat attcacgata aataatcctg
    18841 atggacaact tgctttaatc acaagttata ttccgttttt agcgccattt caattagttg
    18901 tacgcgctca aacttcaggg cttcaaatat atgaggtcat cttaagttct ttaatctcaa
    18961 ttgtaatggt agctgtcttg atatggattg caattaaaac atataaagat tcagtactta
    19021 catttgaacg tggcttattt aattcaatga aacgtgtatt taaaaaataa aattgatgag
    19081 agtgacacac atttatcata taaaatgatg atgaaagcgt gttactctta tttttttata
    19141 tttagctata gaataatgtc taaaaaagtg tacaatatga atgtgaaaac gaacttttta
    19201 aaatgggaat gtgaagtaag aagaaatgta aacaaatgat atgtagccga tagattattg
    19261 atagcaaatg tgtgatggag tgatttcatg ttatatccga tttttatatt tatattagct
    19321 ggcttatgtg aaataggtgg gggatacctg atttggctgt ggcttaggga aggacagtct
    19381 tcacttgttg ggctaatagg cggtgcgata ctcatgttat atggtgtcat tgcgacattt
    19441 caatcatttc catcattcgg aagagtatat gcagcatatg gtggcgtatt tatcatcatg
    19501 agtttgattt ttgcgatggt cgtagataaa caaatgccag ataaatatga tgttatcggt
    19561 gcaatcattt gtattgtagg tgttttagta atgttgctac ccagcagagc ttgaacactt
    19621 gtggttagaa gggagaagta aaaatgatag ttgaagttga gcaccaaaga caaggtgtag
    19681 atttaatcaa aattgataat gatgaaacta aaattatttt tacgaattat ggagcaagag
    19741 tagtgtcttg gaaatatcat gataataata ttgttcttgg aaatgttgtt gaagcggatg
    19801 aattttatca tagtaatcct tttaaatttg gtgcttctat aggaagatat agcggtagaa
    19861 ttgataatgc taaatttaag ttgaaaggta aagaatatca gttagaaaaa aacaacggtg
    19921 aacatcattt acatggtgga tgtcatggtt tggacaataa gttatttgat tatgaaatta
    19981 gaaatgaaat tgctcaaatt aaagttatat ttaagacggt gttgaaatca gctgatgatc
    20041 atttcccggg tgatatcgat gtgacgatta cacatattta tgatgcggat caccaatggt
    20101 cgattgaata cgaagcagtt gcatcagaag acacattgtt tagtcctacg aatcatgtgt
    20161 acttcaattt gaatcgagat aataatgtcg ttgataatca tcgcatttca agtaatcaat
    20221 tagatatgta tgtattagat gagcgaaata ttgttacagg tgatattcta gatttacatg
    20281 aagtgtttga agataacaag ataaaattga gtgatatttt tacatcacaa catgcgcaat
    20341 taagccaaca aatgacacgt tttggtggtc tcgatcaccc atttacagtt ggtgaacata
    20401 aaatgtatgt tgaaaatcat gaatttatgt tagaggtcga tacagatatg cctcatgttg
    20461 tgttctttac ttttaaccag cctgatgagt gggatagtcc atttaatatt tataaaccac
    20521 attctggctt tacattagaa acacaatttt taccaaatga tattaattta tatggtgaaa
    20581 aagcaccgtc aattttaaaa gctggtgaag tgttcaattc gaaaacaagt ttccgtatcg
    20641 aagaaaaagt agaaaatgat taaaagtatc ggcatttata taatatttat gttatttggc
    20701 ggaattttga ataggttatc aagaatcgta tcaaatgaag tgacatcatt aatatacagt
    20761 ttgaaataaa gaaaatatat aacataaaaa aggaatcaat agcatcagtg gttatagata
    20821 agactattat acaaacgatg tgtaagttga ttcctttttg ttttgaaaag ttcatttaac
    20881 acttataatg aaaggtaatt atttcaatgt tctgttatac attttttgaa gtttatcgcg
    20941 tcttgttggt gacagaaatg gattgtttag tgcatatcgt aacatatctt gattttgtct
    21001 atcattataa taaaggtcat taagctgttg tacagataaa cgatgttcga ttggtgcttc
    21061 ttgtattaag taaagctgat catttggaga tacatagcct tgttttaata cccggaaata
    21121 gaaacctgtt ttaccagatg tagacatgcg cttcactaaa tcaggaatat tatattttgc
    21181 ttgaattttc cagcatggtt ctctaatttc agaaacctca attaacgctt cacctaaacg
    21241 atattgatta ccaaagtaaa catcagattc gtctaaataa tcaaatgtta aattctctcc
    21301 aaacatcgca tgagtaggta gtgtaggtaa atcatcttga tacagtgcat aattatgctt
    21361 actaaaccca caaactgctt tatgtggtcc accatgatct ttataagcct gttcatcacc
    21421 agtgaaacca gttgaagata accatgttgt ttgtgaaatc ttagttttat ctaaagcaga
    21481 gcgcatcggt cttttcgagc tataaggcaa atcttggatt tttcctgtcg aaattgcgtg
    21541 aattgatatc atatcattct ccttaacgag tattatttaa aatatattgt actataaaac
    21601 atttttagaa ttgtaaattc tgattgatat gtttaaaaaa atgttagaaa taagggtatg
    21661 ttaatttata cgagtcatgt aaaatatgaa ttgaatagat tacataaata tgggaggcta
    21721 ccttcatgaa agatgtcaaa gcactcaagt taatgacatt aaatgatgtt ttaagtcaaa
    21781 tcaatggtga catgacgctg ggaattggta caggaagtac aatggaatta ctcttacctc
    21841 aaatggcgca actaattaaa gaacgtggtt acaatattac aggtgtatgc acttctaata
    21901 aaattgcatt tttagcaaaa gaattaggta ttaaaatatg tgaaatcaat gatgttgatc
    21961 atatcgactt agcaattgat ggtgctgatg aagtagatcc atctttaaat ataattaaag
    22021 gtggcggtgg tgcgctgttc agagagaaag ttatagatga aatggcatcg cgttttgttg
    22081 tggttgtcga tgaaacgaaa atagttcaat acttaggtga gacgtttaag ttaccagtag
    22141 aggtggataa atttaactgg tatcacatat tacgtaaaat tgaatcatac gctgatataa
    22201 aagtagaacg tcgtgtaaac gaagatgtcg catttataac tgataatggc aattatattt
    22261 tagattgtaa gttaccaaaa ggaattgacc catataagtt ccatgagtat ttaattcatc
    22321 tgacaggtgt gtttgagaca ggatacttct tagatatggc agatcaagtt attgtaggca
    22381 ctcaagaggg tgttaaaatt ttagaaaaat aatatatagt atagaaaagc gcttgctagc
    22441 atcatctttt taagaggtac tagcaagcgc tttgttatta tttgttttta tctgaagatt
    22501 gttcttcagt tttttcgata tgtttttctg catcaattac aactgatgaa cgtctgtctt
    22561 ctttataagt cgcaggttct tttaatgatt gctctatttc atcttcattt gaatcgtttg
    22621 ttaaatcgtc actttgtggt gcttgtttaa ctgattctgc tgattctact gactccgaag
    22681 tgatgtcatg atcttcatca tgattagatt cagtttgatt acctatgtga tgttgaggtt
    22741 ctgttttttc agtaccttca tgaatgcttg gttcgtcagc cactgtattt tcagtatcat
    22801 ttttagcaac accaaccgtt gatgcagtag ctacacctgt tttttcagca tctttaatat
    22861 ttgaagaaga tttttcagcc tcagtatgat tagtttcttc atcgtcattg acatggtcta
    22921 aataattatt aggctcaagt tcttcaaggt ttcgtcttgt tgttaaatat gcaatacctc
    22981 ggataattaa gcttaaacca atgtatccta ctgcaacgat tgctgttgaa atggcgatga
    23041 ctttgattga aaatagatcg ccgatttctt cgctaaacaa gaaaataagt ccgaatgtca
    23101 ttgaagcatg gaatgtcgtt gcaatataaa ttgtacgtcc tttagtcgct ctaattaatt
    23161 caccaagaat catagagaat gagaatgtat aaaggaagtt ataagcagca aattctgtac
    23221 cataagttgt atttgctgag aaaactgaat acatcaaacc aacaacaata cttgcaaaga
    23281 acgtgttcat tttcgtttca acaatatttt gtaagtatga acggaatccg aattctacta
    23341 cgaacgccat cagaatatgt ccaatcagaa tgtgtgtaat aggtactgat aagcctgttg
    23401 attgtaataa aataaagcta tctgcaaatg tattaaagct gtacatacca ataattagaa
    23461 taattaaagg taaaattaaa gctagcaata gacgttcaat tactttcagg ctgattgaga
    23521 attttaaacc tgcaagttgg acctttttat atttgaaaac gagtatacaa atgattgcag
    23581 caattaatgg tgctagatct gtaacttcaa atataaagtg tttgacacca attatagact
    23641 ggaaatctct gagcataatg gataacgcca ttgtaatgac aaagaagacg aaaatcgtca
    23701 ttgcccattg aaaaccagaa attttattgt tcttcattat atgtaacctc cattaggtaa
    23761 caagcaaaat atcttttagt aacaattata catattacaa gccgagagat aaattgtcta
    23821 tgtcaatatt aatgaaaatg taatagtctt gacgtatttt attaacataa atcagctaag
    23881 tattcgttat tacatagaaa ataggggtaa aagtaatgta gataagtatt tttaagatac
    23941 ggatttaact atgaattgaa ttcaaaattt cctatacgtt atactttaat tgttaataag
    24001 ccacataaat aagaggggga aatgctgtgt acaagcaagg tgaaccaaat ttatggactg
    24061 gaaggttaga tagtgaaaca gacccgaaaa aatttagaca ttttcaaaca gtaacatttg
    24121 aagatttgtc taagctggaa aagagtagta tgccatcagg ggtcggtata ttaggctatg
    24181 ctgttgacaa aggtgttgct ttaaacaagg ggcgcattgg tgcaaaagaa ggaccagatg
    24241 cgattaaaca agcatttgca ggtttgccgg atttgaatca atgtgaaact ttagtcgatt
    24301 acggaaatgt ttatcatgat catgaggaat taattgatac tcaaaaagaa tttgctatgc
    24361 ttgcagcgaa gtcaattgct aatcatagac aaacattttt attaggtggt ggacatgata
    24421 ttgcgtatgc tcaatattta gcaacacgta aagtctatcc aacacaatct attggtgtga
    24481 taaatattga tgcgcatttt gatacacgtg ctgaacaaca atctacatct ggaacgagct
    24541 ttagacaaat tttagaagaa gatgaaaaca cagattattt agtgcttggt attgctcaag
    24601 gtggtaatac gcaaagttta tttgattatg ctaaagagaa aaagattgat tatgtctttg
    24661 cagatgaatt attgagtcac gtatcaccaa caattaaaga tatgattgaa cgttttgtac
    24721 atgaacatga tgtcattatg tttacgattt gtatggatgt cattgatagt gcgtttgcgc
    24781 ctggggtaag tgcgccggca gtgttaggtt tgtatccaca tactgttctt gaattagcaa
    24841 aacgtattat tccaagtgat aaggtgtctt cagttagtat tgctgagatg aatccaacat
    24901 atgacgctga caatagaact gctaagctcg ttgctaattt agtgcatcat tttttaaaat
    24961 aattaaaaat atttatatgt gacgagattt gcattgcagg gcagaatgtg agtatttctt
    25021 aatagaagtt atattaacgt tgatatattt gaaaaataat tgcagtaaga agactcttaa
    25081 gttgtagtca tcttactgct tatttatgtt tgttgaaata gcaaaaataa caactgtatt
    25141 gtttgaaaag ttctcttatt atataataat taattaaaat tttaacggag ttcaagaggc
    25201 ctttttataa tgacacctta tttgtaaaat gtcatatgtg gtttagcctc tttataataa
    25261 tttaatctgt tctcaagtgt gccagtatgt aattctagct tatgaccatc agggtcggta
    25321 aagtaaattg attgtctatc tctaatatct ctaactcttc cttctaaaat attcacgtta
    25381 ttatctttta acctctgatg ccaatattta aattcgctgt catctatagt gaaagctata
    25441 tgtgtatatg aaaagtgaat ttcattacgt ggtatatctt tttcttcatt taaagcaatc
    25501 cataggcctg caagctcaaa ataagcagtt tttttaccag tcaatagcaa tttcccaagt
    25561 aaaatatctc tataaaaatg tattgaatcg tttaaatttc tgactgaaaa gcatatatga
    25621 ttaatagatt ttaacatctg cttcacttct ttcttagaat ttttataata aattattttt
    25681 gtatcgattt ttgaaatgac gtcattaatt tttatacgat gatctgcgca actgcataag
    25741 aggctctaat aacttaagtt aaagagcctc taaaagtcca tataaataat cttggcattg
    25801 ttgtacgaat gtatgtactt gttcggagtg tttgcgtttt ttatggtaat tgatataaat
    25861 ttttcggcct aagtttggac gaattttttt atattctaaa ttagacgtgt gaaatgattg
    25921 gtaataaaat ctcggaatga tagcgtaacc taagccgaga tgaacaaatc caactgctga
    25981 ttcgaatcga tctgtttcta caacgacatt tggacgaata tttcttctat taaaatagtc
    26041 atctaagtgt ttgcgcactt gagaattttt gtttggtaat atgagtggca aattttcaac
    26101 atctacccaa ttttgatttt taaatgtttc cttgggtgct aataaaatgt aagattcctc
    26161 atataaagga atggatctta tatcttcgtg ggttattttt tcatttgtta tagctaaatg
    26221 aatattaaaa ttcagtaatt gctctataga ttgatgttta tcatgtattt catataaacg
    26281 atattgctgt tcagggtagt cggaatggtg ctttcgaatt aaattcgcaa tccattgatt
    26341 cgtagattca agagtcccaa tttttatcct tggttctgat gtaacgctta aatcatacat
    26401 tttttccatc gtggatcgat attgttgaac taattcgctc gcataacgat aaaactgtat
    26461 tcctttttcg gtaatcttga tgtcttttgt tgaacgtgta aataagtcat aacctaaatc
    26521 tgcttccatt tttttaatcg tagcagttaa agatggctgg ctaatatgta aaaattgtgc
    26581 agctttagta aaactattat atttcacgat agccaagaag tattctaact gaataatttt
    26641 catgtgtttt gctcctttta tttatagatt caagctatgg ataattagtt tatttatatt
    26701 tgttggctat attttaacat actataatga atattgaaga taataatgta agcgttttaa
    26761 atttagtgga attaacttct taaaagaaca ggagcgaatt ttatgagaaa aattcaagca
    26821 aaaaaaggtt tgagtattga gtgtaaaggt tgggaacaag aagcagtact tagaatgtta
    26881 tacaacaatt tagacccaga agttgctgaa agaccagaag atttagttgt ttatggtggt
    26941 attggaaaag cagcacgtaa ttgggaagct tttgaagcaa tagagaaaac gttacgagaa
    27001 ttagaatcag atgagacaat gttagtacaa tcaggaaagc cagttgctgt atttaaaaca
    27061 catgaagaag cgccacgtgt attaatttca aattcagtat tagtgcctga atgggcaaac
    27121 tgggatcatt ttaatgaatt agataaaaaa ggtttaatca tgtatggaca gatgacagcc
    27181 ggtagttgga tatatattgg ttctcaaggt attgtgcaag gtacttatga gacatttgcc
    27241 gagctaggta atcaacactt taatggagat ttagcaggca ctgttacact gacagcaggt
    27301 ttaggtggta tgggtggtgc gcaaccgtta gcaattacta tgaatcatgg ggtagcaatt
    27361 tgcgtggatg ttgatgaaac acgtgttgat aagcgaattg atacgaaata ctgtgatgtt
    27421 aaaacagctg atttagatga agcattaaaa ttagcagaag aggcgaaaga acgtggagaa
    27481 ggattatcaa tcgggttagt tggaaatgct gtagacatcc atcaagcgat tctagaaaaa
    27541 ggatttaaaa ttgacattat tactgaccaa acaagtgcac atgatccgct aaatggatat
    27601 gtgccacaag gatactctgt agaagaagcg aaagtattgc gtgaaaaaga tccgaaaaaa
    27661 tatgttgaac tgtcacaagc ttcaatggca aagcatgttg aattaatgct tgaattccaa
    27721 aaacgtggcg ctgtagcatt tgattatggt aacaatattc gtcaagtagc cttcaataac
    27781 ggagtgaaaa atgcttttga cttcccaggc tttgtaccag cttacattag accattattc
    27841 tgtgaaggta aagggccatt ccgctttgct gcgttgagtg gtgatccaaa agatatcgag
    27901 cgtgcggatg aagaaatgcg caaacttttc ccagaaaatg aaaagttatt aagatggctt
    27961 gatttagctg aagaaaaaat ttcatatcaa ggactaccat cacgtattgc ttggttaggc
    28021 tatggagaaa gagcgaaaat gggcttagct ttaaatcgtc ttgtacgtga cggtgaaatt
    28081 tcagcaccaa ttgttattgg acgagaccat ttagatgctg gttcagttgc tagtcctaac
    28141 cgtgaaacag aaagtatgaa agatggtagt gacgcggttg gtgattgggc tgtattgaat
    28201 gcactcatta acacagctgc aggtggttca tggatttcat tccatcacgg tggcggtgtt
    28261 ggcatgggat attcacttca tgcgggtatg gttgttgtag cagatggatc agagcgtgct
    28321 gaaagaagat tggaacgtgt attgacgact gacccaggta tgggtgttgc ccgacatgtt
    28381 gatgctggct atgacatcgc tattcaaaca gctaaagaaa aaggtattca tattccaatg
    28441 attgataaag caggtgataa gtaatgaatg atttaataat taatcatata gcagaattaa
    28501 ttttaccgag atcaacagat aaacctttga agggtaagga attagatgaa ttaaatgttg
    28561 tgaaaaatgg tacagttgtc attaaagatg gcaaaattgt atatgctgga acacatacag
    28621 atgactacga tgcgactgag acgattgatg ctagtgggaa ggtagtgtcc ccagcattag
    28681 tagatgcaca tacccattta acatttggtg gatctcgaga acatgagatg tcattaaaac
    28741 gtcaaggtaa gtcttacctt gaaatattag aaatgggtgg tggcatatta tctaccgtta
    28801 atgctactag agaaacatcc gaagatgact tgtttaaaaa agcagagcat gatttgctta
    28861 ctatgattaa acacggcgtg cttgcagttg aaagtaagag tggttatgga ttagatagag
    28921 aaaatgaact gaagcaattg aaagtgtcta atcgtttagc tgagaaatat gatttagaca
    28981 tgaaacatac tttcctaggg cctcatgctg tacctaaaga ggcaagttca aatgaggcat
    29041 ttttagaaga aatgattgcg ttacttccgg aagtaaaaca atatgcagac tttgcggata
    29101 ttttctgtga aacaggtgta tttacaatag aacaatcgca acattatatg caaaaagcca
    29161 aagaagcagg ttttaaagtg aaaatacatg cggatgaaat tgatccgtta ggcggactgg
    29221 aattagcaat tgatgaacaa gcaatatcag cagaccactt agtagcttct agtgataaag
    29281 gaaaagaaaa gttgcgaaat agtgatactg tagctgtcct cttacctgca acgacgttct
    29341 atttaggtaa agaagattat gcagatgcaa gaggcatgct tgacaataac ggtgcgattg
    29401 cattagcaac tgattataac cctggtagta gtgtcacaaa caacttacaa cttgttatgg
    29461 cgattgcagc cttgaaatta aagctatcac ctaatgaagt ttggaatgct gttacggtca
    29521 atgctgctaa agcaatagac attaacgcgg gtacaattaa cacaggtgat aaggctaatt
    29581 tagtaatttg ggatgcacca aatcatgaat atattccata tcattttggt atcaatcacg
    29641 cagaaaaagt tattaaagac ggtaaggtca tcgtagataa cacagtatct tttaaagcat
    29701 aaaataatta gaaagctcta agcattggtt taaactaatg tttagagctt tttgtggttt
    29761 gtaaaaacaa gtatattcga gaacacgttt ttgtatagag tctcaattat aaaagttaga
    29821 atagtttgac aacagtgtta cgatattatt aaacaactgt ctatcattaa cgaaagcaat
    29881 taacaagagt acattgttgt gaaatcatcg tttaatagtc aaagatgatc atcgtagggt
    29941 agacaacata tcattgactt aaggaagggg aatgaccgat gggattaaaa gatcaagctg
    30001 ttacctggag aaggtatttt catcaatttc cagaactatc cgataaagaa tttaagacta
    30061 cacaaaaaat taaagatatt ttaacagaac atcatattag aatattagac ttaccacttg
    30121 cgactgggct tgttgctgaa gtgggacaag gtctgagttg tatagctgtt cgggctgaca
    30181 ttgatgcttt accgattcaa gagcttgttg aacaggattt taaatctgaa aatgaaggtg
    30241 ttatgcatgc gtgtggtcat gatattcaca tggctagtat tttggctaca gctgtaaaat
    30301 taaaagaaat tgagggcact cttactgggc gtgttaaatt catttttcaa tcagcagaag
    30361 aactaggaca tggcgctttt aaaattattg aaacacatgc attaaaagat gtacaagcag
    30421 tattgggttt tcataatgac ccatcgcgtt cggtaggtac atttgcaatc aaaacagggg
    30481 caattacatc agcagtagat cgttttgagt ttcatattaa aggcgtgggt ggtcatgctg
    30541 caaagccaga acaatgcaac gatccagtta ttgtgttggc gcaattgatt aatagtattc
    30601 aatccatagt tagtagaaat ctatctgcgt ttgatgaagc ggtagtaaca attggacaaa
    30661 tatcatgtgg taatacttgg aatgtcattg ctgatcatgc gtatgtacaa ggcacagtgc
    30721 gtagttttga tcctgtggta cgaaagcttg tagaaacacg attacaagat attgcggacg
    30781 gattggctca agcatataat atgaaaatca atttaaatta tacgcattta cctggcgctg
    30841 tgatgaatga tgaggcattg acgcataaag caattgcagt agcccaacat gttggatata
    30901 aggtggaaat gatggaacag ccgcttacga ttggagaaga tttttcaggt tatagtcaac
    30961 attttccaag tgtatttgcg ttaattggtt ctcatagtga atatgattta catcatcctc
    31021 aatataaacc ggatgaaagg attctggaaa aagtacctga gtattttgtt gaatttgtaa
    31081 agagactatt gcatgaataa ttcgagcttg tttttataaa gaagaaagta atttttagtg
    31141 taatcatgat taagttccta ggaagattgg gtaaataata accattaagc gaataaggag
    31201 atgtaaaaaa atggcagctc aagatcctag aacaaaattt aaaacaacgg attatgaaaa
    31261 acaagaacaa gaggtaccgg gtttacaatc tgaaatgacg ccagcacccg attgtggcga
    31321 aacgtcttac caaggtcatc aacgcttaca aggctataaa atattagtca caggtggtga
    31381 ctcagcaata gggcgtgcag cagcaattgc gtatgctaaa gaaggtgcag atgtagcgat
    31441 taactatctt ccgagtgaag aacaagatgc acaagaagta cgccaagtga ttgaagaaag
    31501 tggtcaaaag gcagtgttaa ttcctggtga tataagagat gaacagttca actatgacct
    31561 tgttgaacaa gcatatcaac aattaggtgg cttagataat gttactttag ttgctggaca
    31621 tcaacagtat catgatgata ttcatggatt tacaactgag gcatttacag aaacatttga
    31681 aacgaatgtc tatccgctat tttggacagt tcaaaaagca ttggaatatt taaaaccggg
    31741 tgcgtcaatt acaacgacat catctgtaca aggttataat cctagtccta ttcttcatga
    31801 ttatgccgct tcaaaagcag caattatttc attaactaaa agtttttcag aagaattagg
    31861 accaaaagga attagagtga actgtgtagc gcctggtcca ttctggtcac cattacaaat
    31921 ttctggtgga caacctcaaa gtaaaatccc aacatttggt caaaaaacac ctttaggtcg
    31981 tgcaggtcaa cctgttgagt tatgtggaac ttatgtgcta ttagcgtcag aagaatcaag
    32041 ttatacaacc ggacaagtgt tcggtgttag cggtggagtg caaatagatt aggtttgtat
    32101 gaagctttta attgaacaac ataatattaa tttagttact tcaatccatt aaattaaaat
    32161 gaattggtga agtgtatttt aaatagtcgg taaaggtgtg tatctttatc ggcttatttt
    32221 aatactctgt cataaggagc gtttgctcag ggtgtggatt ataagtttta agcagaataa
    32281 actttcttaa tgaggatgtc aggacaacaa cgaaaaagtt aaaaactgtt gcgggattgg
    32341 gtttagggac ggttagtgga agaaagttaa attcattaac aagtgaaatg cctaaggcta
    32401 gatattaaac gtcagaacgg acaaagaata ataggatatg ttttcaatta aatagcgaag
    32461 tatttatatg ttaagatgat gtaaattaca tagaaacgct acccgtagaa gtactgatca
    32521 tagaaacgct gataaacgta aatttgcaaa ctcgatatta aacaaagggg ttgttattat
    32581 ggcattaata gtatctagag ctatattatt tttgatttta gcattagctt ttggaatagc
    32641 gactgttttt cctgaacaaa aattaccatt atatatagta ttcacattat ttgtagcagt
    32701 tgcgatcgtt tcgaatgtat ggtttacaca tcaaaacgtt aaaaagcata tcattaagtt
    32761 taacaaatac gtcgcatact ttttatcctt tgtctcaggg ttactttaca ttggatttgt
    32821 aatttataca ttaactatag ggagctatga tgttttacca attattatat ttgcaataag
    32881 tatcgtattg gttcaaatat tagaaaatat actgggaatc agtataaaaa atacgcgtct
    32941 atcaaacaaa cagttgaaag tcatctccaa gcaacgtgtt cagcaattta aagtcatatc
    33001 attaatatta gtaagtgtgt ctatcgtgtt catttttatt gcattaatgc ttaaatggtg
    33061 gcttttactt gttattagca ttattgtttt gattattatt acagtagcaa tgattgttta
    33121 tgaaaaatat tatacgactc ctgattaatt tatgattgac gtactaagga ttatcttgta
    33181 acgtaaacgt aaataagcat cacttatcta gagaggtgga gggactggcc ctatgaagcc
    33241 tcggcaacat ctcgaatgtg ccaattccag taaccgtaat ggtttgaaga taagcaggta
    33301 aagcacatga aaacctcttt cttcatcgtt tgtgagaaag aggtattttt aattggaaag
    33361 caggtaaaaa ggatggaagt acataaaaag agcaatgctt gggcattatt ccccttgtta
    33421 ttatttgtgg cgttgttttt aggcgtaggt attatcacag gtgattttac ttcaatgcca
    33481 ttaaatgttg caattacgat aacggtaatt gtggcgttat taatgaatcg aaaagaatca
    33541 tttgctaaaa aagttgaggt cttcacgaaa ggtgcaggcc attcaaacat tgttttaatg
    33601 atgttgattt ttattttagc aggtgcattt tcaaatacag ctgaaaaaat gggtggcgta
    33661 aagtcgacag ttaatttagg actatcatta atcccagaaa atttaattat tgtaggattg
    33721 tttgtgattt gtatgtttgt ttctatatct atggggacgt ctgtaggaac tgttgctgca
    33781 atagcacctg ttggttacgg atttgcacaa gcgacagatg taccaactgc gctagcaatg
    33841 gctacagttg ttggaggtgc aatgttcggc gataatctat caatgatttc agatacaaca
    33901 attgctgcgg ttagaacaca acatacgaaa atgaaagata aattcagagt aaactttaaa
    33961 attgtattgc caggtgctat tttaacgatt attattctgt atttcttaac gaatggcatt
    34021 tctttaaatc atgctaaaaa ctatgattat aatctaatta aagttgtacc gtatatctta
    34081 gtattggtat tagcattatt aggtgtaaat gtcattatcg tattgattgg cggaacatta
    34141 ttagccggaa tcattggtct catagatggt tcattcggtt ggatggggct actagatgca
    34201 gtgtccaaag gtattataag catggaagat attgctatga ttgcgttgct aattggtggt
    34261 ttagtcggta ttattcaaca caatggtggt attgaatggt tgttgcaatt tgttcgctct
    34321 aaagtgaaat caaagcgtgg cgcagaatta ggaattgcta gtttggtaag tgtcgcagat
    34381 attgccacgg caaacaacac catctctatt attatgtcag gtcctttagc taaaaatatt
    34441 gcagatgaat atgatgtaga ttcaaggaaa tcagcaagta ttcttgatat atttggaggc
    34501 tgcttccaag gtttattacc atatagcccg caagtcattt cagcggctgg tgtagctggt
    34561 atttcaccgt taatgttatt cccatacagc atttatccta tattattagg tgtatgcgga
    34621 cttattgcga ttttatttaa ttttccaaag ctaaaaaaga acactagtca agaaatgaaa
    34681 cattaatgct taacaatata tcgatgtagc caagtaaggt ggcttgttat attaacagat
    34741 catgttgtac tcaccgtata acgctaatct tttaaaaatg atgatcatct atgtttggaa
    34801 ctgagcaaaa atacgttaaa ataaaagtaa gagcgaaagt gggtggacaa cagtggcaaa
    34861 atttaatgta gagaatgaac atgtcgaagt tgaaattgaa aagctttata aattttctcc
    34921 agagttagta tacgaagctt ggacaaagaa agatttgttg aaacaatggt ttatgacatc
    34981 agccagaacg aataaagaga ttgaagcgga cgttaaagaa ggcggtaaat atcgtattgt
    35041 agatcaacaa cgtaatggca aagtaaatgt gattgaaggc atttatgaat ctttagttat
    35101 ggatgaatac gttaagatga ctatcggtat gcctggacta agtgaaacgc aagatgtcat
    35161 tgaggtagaa ttttttgaac gtgaaacagg tggcacccaa atgctatttt attatcgttc
    35221 attagttgaa aaagaaagac gatttactaa tttagaatac aagcaaaaga aaaaagaata
    35281 tcatgatgcc atggtgcatg gtttcgaatt gatgtttgat aagatgtacc acgttatcga
    35341 aacatcaaca caacaataaa atgataaaag gaagttatga taggctcaag gccattaaat
    35401 cataacttcc ttttttctaa tttgaaatat cattatgtag taaatactca ttatattttc
    35461 ttacaagtgt ttcaaagaat atatgataac tgatgaagct agaatagtct gtaccatcag
    35521 ttaagtagaa tgtgttactg gatacataga cgttgtaatt tgctttttga gcaagggtat
    35581 tatctttcat tgtagtaaca gaaataaagt atttttgtct aagttgaagc aagtttgtta
    35641 cttctaataa ttcatgtgtt tcacctgata atgaaataat aaagaataaa tcattaggtt
    35701 cactcttgtt caaaatcatt ttaagttcat gttcatcatg aagtacaatg acatgattat
    35761 gtaacgttaa caatatacgc tgtgcctctg aagcaacgtt taactgtgca cgccctgtac
    35821 catacaaata tattgttgaa gctgtatgca tttttttagt aagaagctgg taatcaacgc
    35881 gttctaaata actgaatgta ctatcgattt cttgtttgaa actatcgata gagttagaag
    35941 gtaattggtg tgtcttatta ctttcaaatt taaggtacga tttaaaatca ctataaccgt
    36001 caaaacctaa tttgcgtgta aatctgtgaa tggttgcatt cgaggcatgt gtgaattgcg
    36061 ctaaatcttg tattttcata tttttacatt catctatatg tgtattgata aagtgagcga
    36121 tatgtaaatc attatcatta agtttatcaa agtttcgatt aatgtgttca tctaaaaaca
    36181 tattcaacct ccttgaaaat aaatgtgtaa tagatcgtta ttacattaaa atcttgtatg
    36241 tcattatata tatttgtgta ttgatatagg tttgattatg cgtttaaaac gaatgtatta
    36301 caaatacaac gatgtcatat ataaggtcgt gaaaaaattt tcactatatg aaaagataat
    36361 ctgattatac aaaatacttt caaaatacgt caatgtcgtt gtaggtgtgt aagcgtttac
    36421 tatatattat atttaaatag taagacatct atttataaat gaatctaatt agatgtattg
    36481 ttaggaaaga tgatatggag ggaatagaag atgaatgcga taaagcgttt cggtagtgca
    36541 atgattgtac cggtattaat gttcgctttc tttgggatag tgcttggctt tgcgacatta
    36601 tttaaaaatc ctacaatcat ggggagttta gcagatcaac atacgttttg gtttaaattt
    36661 tggtcagtaa tagaatctgg tggctgggtt atttttacgc atatggaagt tgtatttgtt
    36721 gtaggtctac cactttcttt agctaaaaaa gcaccaggtc atgcagcact tgcagcattg
    36781 atgggttact taatgtttaa tacatttata aatgccattt taacacaatg gccacatacg
    36841 tttggcgcta atttagaaaa aggcgttgaa aatgtacctg gtttaaaatc aattgcaggt
    36901 attgccacat tggatacgaa tattctaggt ggtattatta tttcagctat tattacttgg
    36961 attcacaata gatactatag taaacgatta ccagaaatgg ttggtgtatt ccaaggttta
    37021 acatttgtag ttacgatttc attttttgtg atgttaccat tagcagcaat tacttgtgtt
    37081 atttggccaa ctgttcagca cggtattggt tcaatgcaac actttatcat tgcttcaggc
    37141 tatatcggtg tatggttgta tcacttctta gaacgtgtat taataccaac aggattacat
    37201 cactttattt atgcaccaat tgaagttgga ccagtagtag tcaatcacgg cttgaaagct
    37261 gaatggttac aacatttaaa tgagtttgct aagagtacta agccattaaa agagcaattc
    37321 ccttatggct ttatgcttca aggaaatggg aaagtatttg gatgtttagg tattgcttta
    37381 gcaatgtatg ctacgacacc taaagaaaat cgtaaaaagg tggcggcact acttattcct
    37441 gccacattaa cagcagtcgt tgtaggtatt actgagccat tagagtttac attcttattc
    37501 atcgcaccat acttatttgt tttacacgca gttttagcag catcaatgga tactttaatg
    37561 tacgcatttg gtgttgtagg taacatgggt ggtggcttgt tagactttat ttcaacaaac
    37621 tggttgccat taggtaaaga acattggggt acttatgttg cacaagttat tatcggtctg
    37681 atatttgtag cgatttactt cttcttattc agattcttaa ttttgaaatt tgatattcca
    37741 ttacctggac gtaagaaaac agaagaggaa gttaaattat tttctaagca agattacaaa
    37801 aataaaaaag gtgatagcgt agattctaaa cgtgcttcat ctggtaatga atacgaagac
    37861 aaagctgcat attacctcga cggtttaggc ggcaaagaaa atatcaaaga tgttactaac
    37921 tgtacaacaa gattacgctt aactgtttat gatgaaagta aagttgcaga tacggaatat
    37981 ttcacgcatc aacaaatggc acacggatta gtaaagagtg gtaagagtat ccaagttgta
    38041 gtaggtatga cagtaccaca agttcgtgaa gcatttgaac aaatggttga ggatcaatcg
    38101 tccgaagata aataatacaa aaaaagacac atcactatac gcaatcattg taaatgatca
    38161 tcgtatagat gtgtctttta ttatgatata cgagctttgc tcatagatga cgatctataa
    38221 ttggcagata gaatgtcgat atttctagtt gcaatcgtat gtaacaattt agcaaatatt
    38281 tctggcgtac atataatatg tgcatgagac ttattcaacg tataagtgat atcagtgtga
    38341 ttttggaaca tattcataat atcgatgact ttgtctttaa tgaaagcatc ttgcaagcca
    38401 tcaatattga tatcgaaacg ctttgcagat tgatataaat tatcagaatc aagtaattca
    38461 tttattttct ttggtgaaag tacttggaaa atactatact gatgaaaact taaaaatgaa
    38521 atgaagtatg gaagttgttg ctttggtaag ctaatttcta atacatactg attaaactgt
    38581 ttgtttattg agaattgcaa cgcatcacaa aattgtacac ttttgcataa cttacttaat
    38641 ttttttctag tagtacggtc atttaaaccg gcgatgttga tgatgtatgt ttcgaattta
    38701 tcaattaaca tgtgaggacc tcccgaggaa tacatggcat taaatacacg tttaatattt
    38761 ataaaggtga cttaattttg ttcaagttga ttttaccacg ctttttttct ttattcacta
    38821 agacttttga atgaagttta aaataattgt ttatcagtga taaaatattt gcaataagaa
    38881 gagaatgact aaataatctt aattttcaga aaagtaattg taaccttact ggtcttatgg
    38941 taatattttt caatattatc gacgaggatg tgttaacaat gtttcaaaaa ttgagtatat
    39001 ttgccacgaa gagttttttg gtatggatgt tagtagcagc tgttattgga tttattttcc
    39061 cacaacatgt tgcgacatta ggtaaatggg taccttattt acttggtatt gttatgttag
    39121 gtatgggatt aacaattaca cctaatgatt tcaaaatggt ctttaaagca cctagagcag
    39181 taattattgg tgtctgtcta caattcagta ttatgcccac attagcattt ataattgcaa
    39241 agtcttttca tttaccacct gatattgctg ttggcgtaat attagtcgga tgttgtccgg
    39301 gtgggacatc aagtaatgta atgagttatt tagccaaagc taacgtagca ctttctgttt
    39361 ctattacgac ggtctctacg ttgctagcgc cattcgttac acctgcgtta atatatctat
    39421 ttgcaaatga atggttggaa gtatctttcg tgagtatgtt gtggtcagtt gttcaagttg
    39481 tattaattcc aattgcttta ggtattgttt tgcaaattat taatcgtaaa attgctgaaa
    39541 aagcttctac agctttgcca attatatcag ttgttgctat ttcattaatt ttagcaatag
    39601 ttgtaggtgg cagtaagcac caaatcttaa ctacaggatt attaatattt ttagtagtta
    39661 ttttacataa cgtattaggg tatacgattg gatattggtt agctcgtctt ttaaaattag
    39721 atcgacaaga tcaaaaagca gtcagtattg aagttggaat gcagaactct ggtttagctg
    39781 tgtcattagc agcattgcat tttaatccaa ttgcagcagt accaggcgca gtgtttagtt
    39841 tcattcataa tataacaggg cctattttag caaagtattg gtcaaaaaag ttataattgc
    39901 actaatagaa tgaagtggtc atcggactat gttaagcttt gataaagaga aaaaatagag
    39961 gagtaaatat atgtatagag cagttatatt tgatttcgat ggaacaataa tagatacgga
    40021 acaacattta tttaatgtta ttaataaaca tttagagatg cataatgccg atcctataag
    40081 cattgatttt tatcgttctt ctattggagg agcagctaca gatttgcatg accatttaat
    40141 taaagcgatt ggttcggaaa ataaagataa actttatgaa gaacatcatc ttactagtac
    40201 aacattaccg atgattgata cgattaaatc attgatggca tttttaaagc aacgtcacat
    40261 tcctatggca attgccacaa gtagtgtgaa agcggaaata atgcccacct ttaaagcatt
    40321 aggtctagac gattatatag aggtagttgt tggtagagaa gatgttgaac aagttaaacc
    40381 tgaccctgaa ttatatttat ctgcagtaca acaattaaat tatatgccga cacaatgttt
    40441 ggctattgaa gattctgtaa atggtgcaac agccgcgatt gcagctggat tagatgttat
    40501 tgttaatacg aataaaatga caagcgcaca ggacttttct aatgtagatt atgtagcaaa
    40561 agatattgat tacgatcaaa ttgtagcgcg tttctttacg aaataggagg cgtatcatga
    40621 tgggttacat tatattgttt tttctagctg gtccagtaat tttaggcgtt ggaaatttgg
    40681 tgattggtcc tatatttaac aaacagacac catttcgcgt gcaagtaaga tcttttgttg
    40741 ttggttcaat gatttactta atactcgcaa caattggcta ttttttacta ttacaaggta
    40801 aactttaacg agaaaaccac cttacctcat taaatggacg accatatgta tgtgaaatgg
    40861 tagaacgttc atgtttatgt atgagatagg gtggtttaaa tagttacata tattttaata
    40921 ataacgtcac gatgataagt acaattaaga taatatctat gcctaccata attgtagctc
    40981 ttgttgcatt acttccttgt tcttttgctg atttcatagc acggtagttt ggcacaaagc
    41041 taataattag taagattaat acaattacac caattaatgc tgttgtcatg atgaacgacc
    41101 tcctttattt ttttcaatca attcccaaat aaacgtagca atcacaccga tgattaaccc
    41161 agtgatgaca ccgatttgta ccgttaaaaa tatggcgccg aaaatgatac caataattaa
    41221 tgcaatcggt aaagtcgtac cgagtttaat cttgcgctct ggagaattaa taatagtaaa
    41281 tactgtaaga caaatgagta tgaaagcaag tgttgcaata atagttcttc caactaaata
    41341 taggatgtca ggtttttcca taccgatata atttatgatg aaaaatgcta cagcaaagag
    41401 taccgatatt tttgtagcac gtagcagtat ttgttttaac attgatatac tcctttttaa
    41461 tattattaaa attatatcat aattaccaag aatagctgaa gttgtatgtg actcaacggt
    41521 acttgagcaa cttttttaat tttttagaaa aatcacaaaa taattgtttg caaagttgca
    41581 aaagcctgct atagtagttc tgtaaacgat tgcatggtat gcaaatatta atgtaccaaa
    41641 atcgataatt tatagtataa ttacggcaat aagttttttt atggatttat ttagtatcaa
    41701 tcagagatgg ggtaagaagt tatggagaac aatgaactac aaaggggatt gagtgcccgt
    41761 caaattcaaa tgattgcact tggtggtacg attggcgtgg ggcttttcat gggtgcgaca
    41821 agtacaatta aatggacagg cccatcagtt atccttgcat atttaattgc gggtatcttt
    41881 ttatttttaa tcatgagagc aatgggggaa atgatttatt taaaccctac aacaggatca
    41941 tttgcaacat ttgcaagtga ttatatacat cctgcagcag gttatatgac agcatggagt
    42001 aatatattcc aatggattgt agttggtatg agtgaggtca tcgcagtagg agaatatatg
    42061 aagttttggt tcccggaatt gccaacttgg attcctggtg ttattgctat tttattatta
    42121 atggcagcga atttattctc ggtaaaagcg tttggagaat ttgaattttg gtttgcttta
    42181 attaaagttg taacaattat tttaatgatt attgctggtt ttggtcttat tttctttggt
    42241 tttggaaatg gtggccatgc ggtaggtatt tctaatctat ggacaaatgg cggatttatg
    42301 ccaaatggta ttgttggttt cttctttgca ttatcaattg taattggttc ataccaaggt
    42361 gtggaactga taggtattac ggcaggtgaa actaaagatc ctcagaaaaa tatcgttaaa
    42421 gcagtgaatg gtgttatctg gagaatttta attttctatt taggcgctat ttttgttatt
    42481 gtttcagtgt atccttggaa tcaattagga gacattggaa gtccgtttgt tgcaacattt
    42541 gctaaaatcg gtattacatt tgcagctgga ttaattaact ttgttgtatt aactgcagca
    42601 atgtcaggat gtaactcagg tattttcagt gcgagtcgta tgatttatac acttgctcat
    42661 aaaggtgaaa tgccgaaaat atttactaaa atcatgagaa atggtgtacc attgtacact
    42721 gttgtagcag tatctcttgg tattttaatt ggtgctttat taaacgtaat tttaccttta
    42781 tatatcgatg gagcgaagag tattttcgta tacgtttata gtgcgtctat tttaccaggt
    42841 atgattcctt ggtttatgat tttattcagt catttacgtt ttagaaaatt acaccctgaa
    42901 gaattggaag gtcacccatt caaaatgcct ggtggcgcag taactaacta tttaacaatc
    42961 ttattcttaa tattagtatt ggttggtatg gtatttaatg ttgaaactag aatatcagtg
    43021 cttattggtg tcatcttctt aacgattgta acgatttatt attttattag atataataaa
    43081 aataatgtaa aagcaaaata gtatgattat aatgtgcgct tatcttatga caaacttggc
    43141 cctagcttat taaagttaaa tagccaaaga atgttatgag taggcgcatt tttttataaa
    43201 tatagcttaa atcgaagttg tagtgtgaaa aacttataag tggcaatatt atcgttagtt
    43261 tttactgtgg gctttaaaat aaaaaatagc attattaaaa taacaaacgc aattgatata
    43321 tggaatgaat ccgttaatca actgcgtttg tttttaatat aagaaattga atggccttat
    43381 caaatcattg gtcatatgcg tatgttatac aagataaaag acatgtaagc tttgaaaatg
    43441 atgcataaat tattatatga catgattaaa gaatttatat aagactgtta atgtttaaag
    43501 ttaatatttg ataaatgttt acgatagtta tctaaagcca ttttagattg tgttatgaaa
    43561 tctaatgatg cgtgataatt taatgcaaca taacgataat ataaaatatc aatagtgaac
    43621 atttgagcaa ataatgaagt tgttgctccc atatgcatct cattttcatc agttttccca
    43681 taagttaaaa caatatttga tgcctgtgct acgggattat cccttgtact agtaattgta
    43741 attataggta tatggtagtc atcaataact ttaaccattg attgcatttc actttgcgta
    43801 ccattgttag taataagaat aacactgtcg ttcgaattgt gagttgctaa taatgtagca
    43861 aaaatatgtg tttcttgaac aagttgaata ttaagaccta ttcttgataa cttttggtat
    43921 aagtcggtag caactacaaa agatgcgcca aaaccatata taaaaatcgt ctcagaacgt
    43981 tttaaacaat gacatatttg atcaatagtt ttatcattta attcgttatt tgcatgatta
    44041 agtgcgcgtg tagtacgtgt atggagttta gttcttaaag attctgtgct ttcgttattc
    44101 attaattcaa cattgtaaat tgatgatgct ttaggaacat atttagatat atttattttc
    44161 aagtcgtgaa aaccgccatc agtaattttc ttgctgaatc gaatgactga tgatgtacta
    44221 atatctaata agcttgctaa gtcttgagac ctcattttaa tcaccttttg aggtgaattt
    44281 agtataaatt tagctatctt tttttcattt ttagtcatat atggatattg actatctatt
    44341 tctgttagta cgtttgacat attaatcact ccactttaac gcaatatatt ttattagcgt
    44401 tggctattgt tgtttaacgc gatggtattt acaacttgta acgtaagaga atgaaaaata
    44461 agctgaatat gttgtttgtt taatgatatt gaattgtaaa actgtaaatt tttttaagat
    44521 gtatagataa aaaatagcat actcccatgt gcaaataata tatcaatata ttgatatatt
    44581 accgtaagtc atactgtata ttcccaattt atacattatg tactcatcta tttaaatctt
    44641 agtaaatgta gttatataag actaaatcta ccatcttaat cattatgatt ataactcgat
    44701 taatgcatct ttacaatttg aagaaacttt ttaattgtat aatttatgta ataaagtgaa
    44761 ttttaataaa taacttgttt aaatgacatt ttatgaaaaa taggttaatg ggcaaattta
    44821 attgcaaata atgcgttaga tagttctgta tgataaaaat taagatattg aattttgtta
    44881 ccatagtcga cttgtcggag gggacgttta ttaagtagtg ttataatttc ataataggaa
    44941 acttgatttt cgtttattat atatgatgtt tcaaagcctt tgaacttatt caattcatta
    45001 gtaactattt ggttaaattt ataaatatgc gtttgaatgt tccaatctgt cattaaagta
    45061 ttatatttaa tcgtatcaat gatttgaatc aaattagtaa tttgttgtct catatactta
    45121 gtttcgttta tcatgtagaa gtacctccaa aaatcaatta ttaaacagga aaggatataa
    45181 cagtggaaaa tgaaaaaaag aaatacacgt ttaaagatat agcatggaga gatttgctta
    45241 taattccaat tataggtgtt atattttatg cactcacaat tgcagcttac ataggaccaa
    45301 ttttgtttgg ttattatgaa atcgatagta atctgatatt tgtagcgact atcacacaag
    45361 ctttagcata tacgattggg attggcgcat tttatttatt tcatttaaaa gtgatgccat
    45421 caaggcttcg agctggtttt gcgtatatca aaaaatattg gcttagattg tcaataacgt
    45481 atattattgc tctcatctta atttatacat atgaatatat gacacagttt ctacctaaac
    45541 atttgcaata tagtgaaaca gccaatgaac ttgagctaaa taaaatgttt gaagtgcctg
    45601 catttttacc tgtagcattc ttattaattg tgattgtagg tccaatagtt gaagaaatcg
    45661 tatttagaca tattttaatt ggagaattag gtaagaagtt taattttata gctatgagta
    45721 ttgtttcggt gttcttattt gcatttattc atgtgactga tgcaaagtcg ccgtttgagt
    45781 ttggtccata tctcatacta tccatcatat tagttttcac ttatttaaaa tctggaagaa
    45841 acctaggttc aacaattgca ttacatatag caaataattt tgtttctttc gttatatcag
    45901 taataagtat ttatgcgtag ataaagaggt cgggacatta agtttttagg caat
//
LOCUS       DALAAR010000014        43539 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000014 DALAAR010000000
VERSION     DALAAR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 43539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.014"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(268..1233)
                     /locus_tag="O2458_000779"
     CDS             complement(268..1233)
                     /locus_tag="O2458_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ3886663.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(1447..3084)
                     /locus_tag="O2458_000780"
     CDS             complement(1447..3084)
                     /locus_tag="O2458_000780"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCZ3886664.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(3244..4122)
                     /gene="hchA"
                     /locus_tag="O2458_000781"
     CDS             complement(3244..4122)
                     /gene="hchA"
                     /locus_tag="O2458_000781"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCZ3886665.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(4388..5575)
                     /locus_tag="O2458_000782"
     CDS             complement(4388..5575)
                     /locus_tag="O2458_000782"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCZ3886666.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            5746..6921
                     /locus_tag="O2458_000783"
     CDS             5746..6921
                     /locus_tag="O2458_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ3886667.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(7203..8387)
                     /gene="tuf"
                     /locus_tag="O2458_000784"
     CDS             complement(7203..8387)
                     /gene="tuf"
                     /locus_tag="O2458_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCZ3886668.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(8604..10685)
                     /gene="fusA"
                     /locus_tag="O2458_000785"
     CDS             complement(8604..10685)
                     /gene="fusA"
                     /locus_tag="O2458_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCZ3886669.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(10808..11278)
                     /gene="rpsG"
                     /locus_tag="O2458_000786"
     CDS             complement(10808..11278)
                     /gene="rpsG"
                     /locus_tag="O2458_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCZ3886670.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(11344..11757)
                     /gene="rpsL"
                     /locus_tag="O2458_000787"
     CDS             complement(11344..11757)
                     /gene="rpsL"
                     /locus_tag="O2458_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCZ3886671.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(11855..12109)
                     /locus_tag="O2458_000788"
     CDS             complement(11855..12109)
                     /locus_tag="O2458_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCZ3886672.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(12246..15842)
                     /gene="rpoC"
                     /locus_tag="O2458_000789"
     CDS             complement(12246..15842)
                     /gene="rpoC"
                     /locus_tag="O2458_000789"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCZ3886673.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(16006..19557)
                     /gene="rpoB"
                     /locus_tag="O2458_000790"
     CDS             complement(16006..19557)
                     /gene="rpoB"
                     /locus_tag="O2458_000790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCZ3886674.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(19772..20380)
                     /locus_tag="O2458_000791"
     CDS             complement(19772..20380)
                     /locus_tag="O2458_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3886675.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(20555..20923)
                     /gene="rplL"
                     /locus_tag="O2458_000792"
     CDS             complement(20555..20923)
                     /gene="rplL"
                     /locus_tag="O2458_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCZ3886676.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(20966..21466)
                     /locus_tag="O2458_000793"
     CDS             complement(20966..21466)
                     /locus_tag="O2458_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCZ3886677.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(21504..21650)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21738..22430)
                     /gene="rplA"
                     /locus_tag="O2458_000794"
     CDS             complement(21738..22430)
                     /gene="rplA"
                     /locus_tag="O2458_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCZ3886678.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(22638..23060)
                     /gene="rplK"
                     /locus_tag="O2458_000795"
     CDS             complement(22638..23060)
                     /gene="rplK"
                     /locus_tag="O2458_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCZ3886679.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(23241..23789)
                     /gene="nusG"
                     /locus_tag="O2458_000796"
     CDS             complement(23241..23789)
                     /gene="nusG"
                     /locus_tag="O2458_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCZ3886680.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(23802..23984)
                     /gene="secE"
                     /locus_tag="O2458_000797"
     CDS             complement(23802..23984)
                     /gene="secE"
                     /locus_tag="O2458_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCZ3886681.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(24040..24183)
                     /gene="rpmG"
                     /locus_tag="O2458_000798"
     CDS             complement(24040..24183)
                     /gene="rpmG"
                     /locus_tag="O2458_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ3886682.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(24298..24867)
                     /locus_tag="O2458_000799"
     CDS             complement(24298..24867)
                     /locus_tag="O2458_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCZ3886683.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(24948..25472)
                     /locus_tag="O2458_000800"
     CDS             complement(24948..25472)
                     /locus_tag="O2458_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCZ3886684.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(25472..26218)
                     /gene="rlmB"
                     /locus_tag="O2458_000801"
     CDS             complement(25472..26218)
                     /gene="rlmB"
                     /locus_tag="O2458_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCZ3886685.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(26226..26630)
                     /locus_tag="O2458_000802"
     CDS             complement(26226..26630)
                     /locus_tag="O2458_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCZ3886686.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(26623..28023)
                     /locus_tag="O2458_000803"
     CDS             complement(26623..28023)
                     /locus_tag="O2458_000803"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCZ3886687.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(28007..28654)
                     /gene="cysE"
                     /locus_tag="O2458_000804"
     CDS             complement(28007..28654)
                     /gene="cysE"
                     /locus_tag="O2458_000804"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCZ3886688.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(29077..30531)
                     /locus_tag="O2458_000805"
     CDS             complement(29077..30531)
                     /locus_tag="O2458_000805"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCZ3886689.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            complement(31088..32161)
                     /locus_tag="O2458_000806"
     CDS             complement(31088..32161)
                     /locus_tag="O2458_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCZ3886690.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(32186..33550)
                     /gene="radA"
                     /locus_tag="O2458_000807"
     CDS             complement(32186..33550)
                     /gene="radA"
                     /locus_tag="O2458_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCZ3886691.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(34035..36491)
                     /locus_tag="O2458_000808"
     CDS             complement(34035..36491)
                     /locus_tag="O2458_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ3886692.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIETAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(36505..37512)
                     /locus_tag="O2458_000809"
     CDS             complement(36505..37512)
                     /locus_tag="O2458_000809"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCZ3886693.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(37502..38068)
                     /locus_tag="O2458_000810"
     CDS             complement(37502..38068)
                     /locus_tag="O2458_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCZ3886694.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(38087..38548)
                     /locus_tag="O2458_000811"
     CDS             complement(38087..38548)
                     /locus_tag="O2458_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCZ3886695.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            38706..39920
                     /locus_tag="O2458_000812"
     CDS             38706..39920
                     /locus_tag="O2458_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ3886696.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(40128..40688)
                     /gene="pdxT"
                     /locus_tag="O2458_000813"
     CDS             complement(40128..40688)
                     /gene="pdxT"
                     /locus_tag="O2458_000813"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCZ3886697.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(40692..41579)
                     /gene="pdxS"
                     /locus_tag="O2458_000814"
     CDS             complement(40692..41579)
                     /gene="pdxS"
                     /locus_tag="O2458_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCZ3886698.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            41683..43065
                     /locus_tag="O2458_000815"
     CDS             41683..43065
                     /locus_tag="O2458_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ3886699.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT        13107 a         8444 c         6363 g        15625 t
ORIGIN      
        1 ctgttcaaca aattttctaa aaattcaaaa ttaattaaca gatttctaga aagactatat
       61 cttttagtat aaacgtatta atttcacaga gacaagtaat ctgtgtttta ctaatatact
      121 ttacatacaa aaaactcttt actttaaaat gaactaagct cgcgaattca ataagtataa
      181 tgaataatat tagaattcat gcactagttt attaaaataa agagtaattt aaaatatcat
      241 tccgtgtatt aaagtgaatg gaaatgatta gttattattt ttaacagtat ctttttgttc
      301 aatagcttct aacattaatt tagtcatgct cgctaaatca tatttaggat caaatcccca
      361 ttcgccacgt gaacagcttg tatcaataga atccggccaa ctattagcga taccttgtct
      421 aataggatca acatcgtaat ctaatgtaaa attgggatag tattcttgaa ttgcttcttt
      481 taccatctct ggatcaaaac tcattgcgct caaattataa ccatttctag tttctaattt
      541 agcgtcgtct gcttccataa gtttaataat tgcttcaatt gcatcatcca tatacatcat
      601 atccatatac gtgcctttat ctatgaagct tgtataatga ccctctctta ctgctttgaa
      661 gtatatttca acagcatagt ctgtagtacc gccacctggc tctttaacat gcgagattaa
      721 acctgggaat ctaacacttc ttgtatctac accaaaacgt ttgaaatagt attgacacaa
      781 taattctcca gctactttat ttacaccata cattgtcgta ggttgctgaa tcgttacttg
      841 tggcgtatta actttaggag ttgagtctcc aaatgcacca attgaacttg gtgtgaaaaa
      901 gtgcaaatta taagttcttg cagcttctaa tgcattcatt aatccaccca tatttaaatc
      961 ccaagctaga attggatttt tctcagcagt tgctgataat aatgctgcca tatgcattag
     1021 actatccgct tcaaagtccc taactaactc aaacatacgg tcacgatctg ttacgtctaa
     1081 gatttcaaat ggtccatttt gtacaggtga gtctgcttca ggttccctaa tatctgtagc
     1141 aagaacatta tctgtcccat aaatttctct gcacttaaca actaattctg taccaatttg
     1201 tcctaatgca ccagtaatca taattttttt catagaaata tctcctttgt ctcttctata
     1261 tagctatagt ccatcacaag cggacataat attcattttc ataataatta taatataaaa
     1321 gcgctttctt gtatatatga catgtacatg ttgctgatat ttctgtaaat ggaaattcta
     1381 gttgtattaa ttgattttag taatttatag cgtttattat tgctaattac tgtcaaatta
     1441 aattttttat ccctcaactc ttaaactctg gatatctttc attatattag cttttttata
     1501 accatggata tcatgtaaag ccttataagc tttaaataat gtttcatacc tttgtacttt
     1561 ttccgcttct ggattaattt cgtataaaat tggctctttc atagcatcaa ctgcgtcatt
     1621 aattgaatta tgtgcgccac cactgactgc acctaatatt gctgcgccta ttgctggtgc
     1681 atactcacta tccataacaa ttagtttttt attcagtaca ttcgcataga tatccatcat
     1741 aacagcattc tttttaggta taccgccaca tgcaaatacc ttttctactt ccatatgcca
     1801 atcttgatac tgttgcataa tcatcttagt accaaatgct gtagcttcta aatatgctct
     1861 ataaatatcc tcatgcttag tttgtaaagt taatccaaag atacaacctg ttaaattgct
     1921 atcacttaat acacttcgat ttccattatg ccaatcaaga gcaatgagcc cactttcacc
     1981 tggcatttga tgttttatct tttcattcat taattcaaat acagtcatat ttctattttc
     2041 tgcttcatct acatatgact ttggtgcttg cttagcgaca tactcaaaca aatcacctac
     2101 tgctgattgc cccgcttcat aagcaaataa ttctggaata attgctcctt ttacagaacc
     2161 tgatatacct ggcacttgat gttgcttttc atttaacata agatggcatg tgcttgttcc
     2221 catcaccata gtcatttctt tatctttttc agacccaata cctaataaac tagcatgggc
     2281 atcaataata aaaggactta ccatagtttc ttttgataat cctaatttct gtgccatttt
     2341 atcatccagt ttccctactg cttcaccaat attaacaacc ggtgcagata ctttatcttg
     2401 aattactttt gataatttgg ggtctatttt atcaaataaa tcataatgaa accctgtttc
     2461 ttcttcccaa aatgctttga aacctaatcc acaattcgag cgtacatttt tattagttaa
     2521 tttatttaca atccaatcgc ccgcttccat aatataagcc gttttttcca taatttctgg
     2581 tgctcgattc atgacctcca ttattttggg aatcatccat tcactactaa cattatatcc
     2641 ataatggcct aaccacttat tattattttc aatagcagtt tgatataatt tctctgcctc
     2701 tttatatgca ccatgatgtt tccaaagttt cacatacgca tgtggattgt ttttaaattg
     2761 ttttaagtta tgtaccgggt taaggttttc gtcagtaaaa ataatagtag atgaagtaaa
     2821 gtctatacca atacctacta tattgtctgg atctattttt gattcacgta ctatatatga
     2881 tattccttct tccataattt ctaaataatc attactattt tgaagtgcat atgtatgtgg
     2941 tattttcaaa ccatttaatt cactctcaat gacaccatgt gtatatggtt tcacaaattt
     3001 tgatactact tgaccgttag ttgtattaat taaaaacaca cggcctgaag cagttccata
     3061 atcaattcca atgctataag acatcatgtt tcctccatat cgctagttgc ggttattagt
     3121 tttgcaagcg ttttcttaaa tcatactcat aataggttct attttacaag tatcacttaa
     3181 attttgccaa aaaaaaagag ccctcataca tttacatatg aggctcctct aattaatatt
     3241 taattattta ttttgtattg catttaacat ttcattaact gctaattttc ctaactcatt
     3301 tgaagctaaa ggactgtcac ctgttaataa tttacgatct tttaacgttc ttcctgtcat
     3361 atcgtcgtta actactttta atccttgttt agttaataaa tcagcaacta accatttcaa
     3421 gcgtccaggt aaataaccta tttcaatatt tgcaccttca tctaatgagt cagggaagac
     3481 acaaacagag tatccttcta atggagattt ttctctgtta agccctgctg aaagtagtgc
     3541 tgctggtcca tgacataatg taactataaa gcggtcattg tctaatgccc aatctaatgt
     3601 ttgttgaacg tcctcacttt cagaaatacc aacaactgca gcatgtccgc ctgggataaa
     3661 gacagataaa tagtctgaat caggtcctaa ttcattttta atcacatctg ctaatttttt
     3721 tggctgtttt aatttttctt tcaatttatt ataagtactt ataactgcct cgtcttcagt
     3781 tggcatagcc cataattcta atttaactgg ataaccagat aatgtcgcaa catcaacgtc
     3841 aaaacctgct tccattaaat gatgtaaagg taataacatt tcaacaggat gattacccgt
     3901 agagaacatt tttccatttt ccaataatac atatcgctct tcagcagcaa tcatcaatac
     3961 tttccattta ccatctttat aggcaccttt gtgttcaaca ccatcaaaat ctgttttagg
     4021 tgctgtatat tgactaaggg aatatggtga tgggaaaaat gcgttatctt ctgctttatc
     4081 tggcgttggt tgcttactta attcatttac atcttgtgac atagtttatt cctactttct
     4141 ctatatagtt actactagta tagacatata ctacacacta taaacatgta ttactattaa
     4201 aatgctcatt atcaaaaata atcaaaatga atttctttga aaaaaataca actaaattct
     4261 acaagatgct tttaaaattc aaacttcaat tttggggagc actaaacatt gatatacgaa
     4321 cttactgtta ataaactaaa cattaaaaaa agcccacgac aatatttgcc gtgggaataa
     4381 ataaatatta aatcaacttc atttcttttc ctactttttc ataagccgca attgcttcat
     4441 ctaacatgtc ttttgtatgt gcagctgtag gcatatttct tacacgtcct gtacctcttg
     4501 gtactgttgg gaaaacgata gatttcacat agacaccttc gtcttttaaa cgcttactaa
     4561 attcttgagt tgttttttca tcaccaataa ttactggtgt aattggagtt tctgactcac
     4621 ctgtatcata tcctaatttt gacaatccat tttttaaata ttgtgcattg ttccataatt
     4681 tatcatgtaa ttcagttgaa tccattaact ttttaactgc ttcagttatt gctttggtat
     4741 ccccaggtgc taatgatgta gagaataaga atggtcgtga ttgtgctttt aaccaatcta
     4801 ttaactcttt tgtacctgct acataaccgc caacgacacc aattgcttta gaaagcgtac
     4861 ctatttggaa atcgatttta tcttgtaaac caaaatgttt aaccgtacca gcgcctttac
     4921 ccataacacc tgaaccatga gcgtcgtcaa cataagttaa taaaccaaat tcttctgcaa
     4981 tttctacaat ttcaggtaat ttagccacat caccatccat actaaaaacg ccatcagtga
     5041 tatacatcac tttattgtat tgacctgatt caactgcttc ttttgctttc gcacgtaaat
     5101 catccatgtc tgaatggtta actcgaataa ttttagcttt agataagcga catccatcaa
     5161 taattgatgc atgattaagc tcatctgata aaatagcatc atttttattc atgacagctg
     5221 aaatagcagc catattacaa ttaaatcctg attgataagc tattgcagct tctgttcctt
     5281 taaattttgc tagtgtttct tctaattcgt cgtgtaaatc taatgtacca ttgattgtac
     5341 gaacagcgcc tgcacctaca ccatgtgtat caatagctgc ttttgcagct gatttcaaat
     5401 cttcatttgt tgctagtcct aaataattat ttgaagataa gttaatgtat gatttcccat
     5461 tgattttgat ttctggtccg tttgcacctt caattgtatc tatttcatta tacaaaccat
     5521 tttcttttag ataatttata ttttcctcta aaaactcatg taatgattga accacagtat
     5581 ataatccccc ttaagtattg tttggtaata cctttatcat acagtaattc tacataactt
     5641 gaaagcctta tcattcatag ataaaatatc tcaaagcaat catttagtac gataatttca
     5701 taaaatgtta tactataaac attattttag aaagtagtga taagggtgtt agattggttc
     5761 caactagcaa ataataaaga aaataaaaca attcaactca gacgttattt acaccaatat
     5821 cctgagttat cttttgaaga atttcaaaca catgattata ttgttaacca attaagccag
     5881 ttatcttgtg atattgaaac tcctatcggc cgcaatggta tcaaagccac atttaaaggc
     5941 ttgggcacag gacctaccat tgcattaaga gctgactttg atgcgttacc tgtcgaggaa
     6001 ttaaacgatg tgccttataa gtctaaaaat ccaggatgta tgcatgcgtg tgggcatgat
     6061 ggtcatacag caattttact tactgtagca gagattttag atgaacataa acatttatta
     6121 gaaggtaatg ttgtattaat attccagtac ggtgaagaaa tcatgccagg tggttctcaa
     6181 gaaatgatag atgctggatg tctggaaaat gtagatagaa tttacggtac acatttatgg
     6241 agtggttatc ctactggaac tattcattca cgtgcggggg caatcatggc ctctccagat
     6301 gaatttagcg tcacatttaa aggtcgtggt ggtcatggtg ccaaacctca tgaaaccatt
     6361 gacccaatag taatcatggc agagttcatt ttaagtgcac aaaaaataat ttcacgtacg
     6421 atcgatccag ttaaacaagc tgtactttca tttggaatga ttcaagcagg caccacagat
     6481 agcgttattc ctgatcaagc attttgtaaa ggtacagtta gaactttcga ttcagatata
     6541 cagaatcatg taatggacaa aatggataag cttctacaag gtttagcaat agcaaatgat
     6601 attaactatg atttgaatta tattaaaggg tatttgcctg tccataataa cgagaaagca
     6661 tatcaagtta ttaaagaagc aacaaatgac ttacatgtac gctttaatga atcagattta
     6721 atgatgattg gagaagattt ttcacattat ttgaaagtac gaccaggtgc attctttctg
     6781 actggttgtg gtaatgaaag taaaggtatt acagcgccac accataatcc caaatttgat
     6841 attgatgaaa aatcattaaa atacgctgta gctgtatttt taaaaatcat agaattagag
     6901 caagtattta aaacaaatta agcatttaaa ttaattgaaa gatatgaatc tgagacataa
     6961 actaatgaaa tgctcccttc aaagtagaca ttgaaaaaat gaaaactttg aagggagcat
     7021 tttacaatgt ccaagactca acaattttat ttaaacagta ttatcagtta taagttttat
     7081 tagtcagtct ttcatcaatt attgttcttc taaatcgaat agccattaca aaatttacac
     7141 attaaaaaaa gacccctcga ttgagggatc ttctttttat ataaaatcta agaaattaga
     7201 aattatttaa tgatttcagt aacaacgcct gatcctacag tacgtccacc ttcacggatt
     7261 gagaaacgag taccgtcttc aatcgcgatt ggagcgatta attctactgt catttcaacg
     7321 ttatcaccag gcattaccat ttcagtacct tctggtaagt gaacaacacc agttacgtca
     7381 gtagtacgga aatagaattg tggacgatag tttgagaaga atggagtgtg acgtccacct
     7441 tcgtcttttg ataatacgta tacttctgct ttgaattcag tatgtggtgt aattgaacca
     7501 ggagcagcta atacttgacc acgttgtacg tcttcacgag caacaccacg taataatgca
     7561 ccaatgttgt caccagcttc agcgtagtct aataatttac ggaacatttc aacacctgta
     7621 acagttgttt tagatgtgtc atgtaaaccg atgatttcaa cttcttcacc aactttgatt
     7681 tgaccacgtt caacacggcc tgtagcaaca gtaccacgac cagtgattga gaatacgtcc
     7741 tcaactggca tcatgaatgg tttgtcagaa tcacgttctg gagttggaat gtaagtatct
     7801 acagcttcca ttaattctaa gattttttct tcgtattgag catcgccttc taaagctttt
     7861 aatgctgaac cagcgattac aggtacatcg tcacctggga agtcatattc gcttaataag
     7921 tcacgaactt ccatttctac taattctaat aattcttcat cgtcaaccat gtcaactttg
     7981 tttaagaata ctactaatgc tggtacacca acgttacgtg ataaaagaat gtgttcacga
     8041 gtttgtggca ttggaccgtc agcagcagat actactaaga taccgccgtc catttgagca
     8101 gcaccagtga tcatgttttt aacgtagtca gcgtgtcctg ggcagtcaac gtgagcgtag
     8161 tgacgtttgt cagtttggta ctcaatgtga gaagtattga ttgtgatacc acgttctttt
     8221 tcttctggag cgttgtcaat catgtcatat gattgtgcaa ctgagtcacc attttttgct
     8281 aatacagtag cgattgctgc tgttaatgtt gttttaccat ggtcaacgtg accgatagta
     8341 ccgatattgg catgttcttt agaacgatcg aatttttctt ttgccattat aaaatctctc
     8401 ctattctaat taaaaataat atttaaattt atctctcatg atagtttctc accatcatga
     8461 gaaattattt atagcgatgc aaaaagaatt ttacaattct tttataatgc attcacccca
     8521 aaaaatcaaa ctataaagat gatagcttac atttaagctc accttgtatt ttaacctagg
     8581 ctagctagtc aaaacaagtt atattattca cctttatttt tcttgataat atcttcagcg
     8641 attgattttg gaacttcagc atagtgatcg aagtacatag tgtaagtacc gcgaccttga
     8701 gtgtttgaac gtaatgatgt tgcataaccg aacatttctg aaagtggtac ataagcatta
     8761 acaacttgtg cattaccacg aggttccata ccatcaacac gtccacgacg agatgttacg
     8821 tcacccatga tatcacccat gtactcttca ggcatttcaa tagttacttt catcattggt
     8881 tctaagatta caggatcaca ttttttagca gcttctttaa gtgctaatga tgcagcaatt
     8941 ttgaaggcca tttcagatga atcgacatca tggtatgaac catcatataa tttagcttta
     9001 acatcaatta aaggataacc tgctaaaaca ccattttcca tagcatcttt aagaccagct
     9061 tctactgatg gaatgtattc acgaggaact acaccaccaa cgatagcgtt ttcgaattcg
     9121 aaacctgcgc ctgtttcgtt tggtgtgaat tcaatgtgaa catcaccgta ttgaccacga
     9181 ccaccagatt gacgagagaa tttaccttga acttgtgcag atgatttgaa tgtttcacga
     9241 tatgaaacca ttggagcacc tacgttacat tcaacgttga attctttctt catacggtct
     9301 actaagatgt ctaagtgaag ctcacccata ccaccgatga taacttgtcc agtttcttcg
     9361 tcagtgtgtg catggaatgt tgggtcttct tcttgtaatt taactaaagc ttgagtcatt
     9421 ttatcttggt cagctttaga ttttggctct actgataagt gaataactgg ctctgggaat
     9481 tccattgatt ccaagataat gtcatttttc tcaccacata aagtatcacc agtacctgta
     9541 tctttaagac ctaccgcagc agcgatatct ccagagtata cagtatcgat ttcttgacgt
     9601 gagttagcgt gcatttgtaa taaacgacct acacgttcac gtttaccttt agtagagttc
     9661 ttaacgtatg aaccagatgt cattgtacct gaatacacac ggaagaatgt taatttacca
     9721 acataagggt cagtcataac tttgaacgct aatgcagcga attcagctga atcgtctgct
     9781 ttcgcgatta cttcttcttc agggttgcta gcacggtgac caataattgg tttaacgtct
     9841 agtggtgaag gtaagtaatc aattacagcg tcaagcatta attgaacacc tttgtttttg
     9901 aaagctgtac cacaaagtac tgggtagaat tctacgttag tagtagcttg gcggatagct
     9961 tcttttaatt cagaaactga aatttcttcg tcaccaagat atttttccat taattcgtcg
    10021 ctagtttctg caactgcttc gattaagcta gcacgagctt cttcagctct atctaagtgg
    10081 tcttcgggaa tttcaatttc ttcaatttca gtacctaaat catttgtata tttgaaacat
    10141 ttcatttcaa ctaagtcaat gattgcttcg aattcgtctt ccgcaccaat tggtaattgg
    10201 attggagcag cgttagcttg taaacgatca tgtaatgtac ttacagagta ttcgaagtta
    10261 gcacctaatt tgtccatttt gtttacaaat acgatacgtg gaacaccata agttgtagcc
    10321 tgacgccaaa ctgtttcagt ttgaggttca acacctgatt gtgcatcaag tactgtaact
    10381 gctccgtcaa gtacacgtaa tgaacgttca acttctacag tgaagtctac gtgtccaggt
    10441 gtatcgataa tgtttacacg gtgaccttcc caagctgctg ttgttgcagc agatgtgata
    10501 gtaataccac ggtcttgttc ttgctccatc cagtccattt gtgaagcacc ttcgtgtgtt
    10561 tcaccaattt tgtggatacg gccagtgtaa taaagaatac gttcagtcgt agtcgtttta
    10621 ccagcatcaa tgtgagccat gataccgata ttacgagttt tttctaatga aaattctcta
    10681 gccatgtatt ttttctcctt ccagtttata ttactgaata aatacgatag atatggcgat
    10741 gccctaagca tgaacgctta atgaaaattc attaatatag aacacactca gggtaaaagc
    10801 ttttatctta ccaacggtag tgagcaaatg ctttgtttgc ttcagccatt ttgtgagtgt
    10861 cctcacgttt cttaacggca ccacctgtat tatttgctgc atctaaaatt tcgttagcta
    10921 aacgatcttc catcgttttt tcaccacgaa gacgtgcata gttaactaac caacgtaaac
    10981 ctaaagtagt acgacgctct ggacgaactt ctactggtac ttgatagtta gaaccaccta
    11041 cgcgacgagc tttaacttct aatactggca taatgttgtt gattgcttct tcgaatactt
    11101 ctaatgcatc acgaccactg cgttgttcaa ctaggtcgaa tgctgaataa agaattcttt
    11161 gtgctgttcc acgtttacca tctaacataa ttttgttaat taatttagtt actaacttag
    11221 agttatgaat tggatctggt aatacgtctc ttttaggtac tgatccttta cgaggcataa
    11281 tgtaaatcct cccttcctta tattaaatat tttaagttta agatttaatt aaaaactaaa
    11341 ttcttagttt ttaggtttct tagttccgta taatgaacga ccttgtctac gtccgtcaac
    11401 acctgaagta tcaagtgctc cacgtacaat atggtaacgc acacctggta agtcttttac
    11461 acgtccacca cgtacaagta caacactgtg ttcttgtaag ttatgtccga taccagggat
    11521 gtatgcgtta atttcgatgt tgtttgataa acgcacacgt gcatatttac gtaacgctga
    11581 gttaggtttt ttaggtgtca ttgtacctac acgagtacat acaccacgtt tttgtggtga
    11641 gtttaagtca gtaaattttt tctttttact gttgaaacct ttatttaaag ctggagaatc
    11701 tgattttttg attttgcttt gtcttggttt acgtactaat tggttaatag ttggcatgtg
    11761 atatgtcctc ctctcttcac tttttaatcc cacacatcca ggtggttcat ttttaggtta
    11821 aataaaattt agtaagtaaa acacttacta attctcattt aatcaatgct actattgtcg
    11881 cattgacgtt aatacctaca tgtttaccca aagcatgttt gcttttgaaa aaagatacag
    11941 gtatattttt ctgattgatt tggcttaaca cgcgagtcat taaatatact tcaacgtctt
    12001 cagcaataat caaagatgta acttgatctt tctttaacgc tttaagcgtt tctttaagac
    12061 caactacaaa atgttgtttg ttaaagcgtg caactttttc cttagacaat aatttatcct
    12121 ccaaagttct gcttgcatca acctttaata tattaacatt gaacaagaga attcgtcaac
    12181 attaaatcaa ataatttaca taaaaataac aagaggctaa agcattagcc tctgttatat
    12241 acttgttatt ccgttacttc agtttgagat tcaacttctg caactggttt agctgttttt
    12301 tcgtatttta cgtcgctata acgtctcata ccagtaccag ctggaattaa cttaccaata
    12361 attacgtttt ctttaagacc taataagtca tcacgcttac ctttaattgc tgcatctgta
    12421 agaactcttg ttgtttcttg gaatgatgct gcagataaga aactttctgt ttcaagtgat
    12481 gctttagtaa taccaagtaa tactggttta gctgttgcag gacgcttacg gtgtttaaat
    12541 gcttctctat ttgcatctgt aaagttatga atatcaacta atgaacctgg taataacttc
    12601 gtatcacctg cttcgataat tctaacttta cgtaacattt gtcgaaccat aacctcaacg
    12661 tgtttatcgt cgatttctac accttgcata cggtaaactt tttgtacttc ttttaataag
    12721 tagctttcag tcgcgtttaa tccagcaaca gataagtaat tcttaggttc aatagaacct
    12781 tcagttaata cttcaccacg ttgaactggt tgaccgattt ctacaataat tcttgaagta
    12841 cctgaagcaa ggtatgatct tgtttcatta gcacctttaa caacaatttc ttgttgtcta
    12901 tcttttgcta atttaatatc ttctacgaca ccttcgattt ccgtaattac cgcttgacct
    12961 ttagggttac gtgcttcgaa aatctcttga atacgaggaa gaccttgtgt gatatcgcta
    13021 cctgctaccc cacctgtatg gaatgtacgc attgtaagct gtgtacctgg ttcaccgata
    13081 gattgggctg caattgtacc aactgcttca ccaacttcaa ctttttcacc agtagcaagg
    13141 tttttaccgt aacatttttc acaaacacca tgtcgtgcgt tacaagtaaa tgctgagcga
    13201 atatacattt gttcaatacc agcatctgta attttcttag caatttcagg tgtaattaat
    13261 tcatcaggac gaatgattat ttcatcagtt tcaggatgat gaattgtttc tttagaataa
    13321 cgaccttcaa tacgttcgat aaatggttca atcatttctg taccttcttt aatatcagaa
    13381 actaataaac ctctatcagt accacagtct tcttcacgaa caataacatc ttgtgccacg
    13441 tcaacaagac gacgagtaag atatcctgag tcagctgttt taagtgctgt atcggcaaga
    13501 cctttacgtg caccgtgagt tgagatgaag tattcaagta ctgttaaacc ttcacggaat
    13561 gaagatgtga ttggtaattc gataatctta ccagatggtg cggccatcaa tccacgcata
    13621 cctgctaact gtgtaaagtt agatgcgtta ccacgggcac ctgaatcact catcatgaag
    13681 attgggttag ttttatcaag tgattgcatc aattcacctt gaatttgatc ttttgcatct
    13741 gtccaaattt caacaactgc attatatctt tcttcttcag tgattaaacc acggttgaat
    13801 tgttttgtaa ttctgtcgac taatttttca tgctcatcaa gtatttgttg cttatcaggt
    13861 aatactacga tatcagcaac acctactgta ataccagctt tagatgagaa tttgaatcct
    13921 aagtctttca tacggtctaa catcattgat gtatcagtga tgctaaatct gttgaatact
    13981 tctgcaataa tattacctaa gaattttttg ttgaaaggtt caattaattc ttcattttca
    14041 aagtattctt ttaatccacc ttcacctaaa gttgtaggat cgatgaaata tctgtttggt
    14101 gtctttcttt ctaagttttc ttgcgtaggt tcattaatat aagcaaatga atctggaatg
    14161 atttcattga atataatttt acctactgac gtagcaagaa tctttttgtt ttgttcttca
    14221 gtaaatgttg gattattgaa cgaacttgca tgtacaccaa ttctagtgtg taaatgtaca
    14281 aagccatttg catatgcttt taatacttca tttgtattat taaagattgc gcctgtattt
    14341 actgcatctt ttctttctaa agtaaggtaa tagttaccaa gtaccatatc ttgtgatggt
    14401 gtaactacag gtttaccatc tttagggttc aagatgtttt gtgctgctaa catcaacatt
    14461 cttgcttcag cttgtgcctc ttttgataaa ggaacgtgaa ccgccatttg gtcaccgtca
    14521 aagtcagcgt tataagctgt tgttacaagt ggatgtagac gaatcgcacg accttcaact
    14581 aaagttggtt caaatgcttg aataccaagt ctatgaagtg ttggtgcacg gttaagtaat
    14641 acaggatgtt ctctaattac ttcttccaat acgtcccaaa cttcatcatc catacgttcg
    14701 attttactct tcgcattttt aatgttagtt gcaatttcac gttgaactaa ttctttcatt
    14761 acgaatggtt taaatagttc aagtgccatt tcttttggta aaccacattg gtacattttc
    14821 aagcttggac ctactgcaat aactgaacgt cctgaatagt caacacgttt accaagtaag
    14881 ttttgacgga aacgaccttg tttacctttt aacatatgag ataaagattt taatggacgg
    14941 ttacctgggc cagtaactgg acgaccacga cgaccattat caattaaagc gtcaacggct
    15001 tcttgtaaca tacgtttttc gttttgaacg atgataccag gtgcacctaa atctaataaa
    15061 cgtttcaaac gattatttcg attaattaca cgacggtata agtcgtttaa gtcacttgtt
    15121 gcaaatcgtc caccatctaa ttgaaccatt ggacgaattt ctggtgggat gattggaagt
    15181 acatctaaaa tcatccatga aggtttgtta cctgaattac ggaatgattc aacaacttct
    15241 aaacgtttaa ttgcacgagt aagtctttga ccagtagctg attccaactc atcgcgtaac
    15301 aatttaagtt cttcgtcaag atcaatctct tcaagtaaat ctttaatacc ttctgcaccc
    15361 atttttgcaa cgaattgacc tgggtattta tcataataat ctctgaattc agcttcagat
    15421 aataaagttt tcttttctaa accagttgga cctggatcta caacaacata agaagcaaag
    15481 taaataactt cttctaatgc tcttggtgac atgtcaagta ataatcccat acgacttggt
    15541 atacctttga aataccaaat gtgagaaact ggagcagcaa gttcaatgtg acccattctt
    15601 tcacgacgta ctttagattt agttacttca actccacatc tgtcacagac catgcctttg
    15661 tagcgaacac gtttgtattt accacaacta cattcccagt cttttgtagg tccgaaaatt
    15721 ctttcacaga atagaccatc tttttcaggt tttaatgtac ggtagttgat tgtttcaggt
    15781 tttttaactt caccaaaaga ccaagaacgg attttttcag gtgaagccaa tcctattttc
    15841 atataatgga aattatttac atcaatcaag gagcctacct ccttcaattt agattagctg
    15901 tgctatttga ttgatttgca tttatttaaa atggcttaaa gtactaaact gaatcatcaa
    15961 taaaattcag cttagtatct ttttgcctgt tttgtaaatt gcgtattaat cagtaacttc
    16021 tttttgtgtt tcaggagcat cattttgttg taaatctact ttgcgttcta caacatcatc
    16081 gtcatcaacg tctgtcattt cgatttcatt atcttgctca tccataactt ttacatctaa
    16141 acctaaactt tgtaattctt tcatcaatac tcggaatgat tctggaacac ttggtctaga
    16201 gatgttttca cctttaacaa tagcctcgta tgttttcaca cgtcctactg tatcatcgga
    16261 tttgtaagtt aagatttctt gtaatgtgta tgcagcacca tatgcttcaa gtgcccatac
    16321 ctccatctca ccaaaacgtt gtccaccgaa ttgcgcttta ccgccaagtg gttgttgtgt
    16381 aacaagtgaa tatggtcctg ttgaacgcgc atgtaattta tcatcaacca tgtgcgcaag
    16441 tttcaacatg tacattacac ctactgaaat acggttatcg aatggttcac ctgtacgtcc
    16501 atcataaagt acagttttac catcacgagc cataccagct tcttcaattg ttgaccatac
    16561 atcgtcatcg tttgcaccgt caaatactgg tgatgcaacg tgaataccaa gatttttagc
    16621 agccatacct aagtgtagct ctaatacttg tccgatgttc atacgagatg gtacaccaag
    16681 aggatttaac atgatatcga tcggacgtcc atctggtaag taaggcatat cttcttcagg
    16741 aacaatctta gaaatgacac ctttgttacc atgtcgacca cacatcttat caccaacatg
    16801 aattttacgt ttttgaacga tatatacacg tactaattgg tttacaccag gtgataatgt
    16861 atcgtcgcct tcttcacgat tgaatacttt tacatcaaga acgataccgc cagcgccgtg
    16921 aggtacacgt aatgaagtat ctctaacttc acgtgctttt tcaccaaaga ttgcatgtaa
    16981 caatctttct tcggcagtta actcagttac acctttaggc gttactttac caactaaaat
    17041 atctccatct tttacttctg caccaatata aacgatacca cgatcgtcta agttcttaag
    17101 tgcactttca gaaacattag gaatatctct tgtgatttct tcaggtccta acttagtatc
    17161 acgtgcttct gattcatact cttcaatatg aatagaagta tacacgtcat ctttcacaag
    17221 tctttcactc atgataacgg catcctcata gttgtaaccg tcccaagtca tgaaaccaac
    17281 tactacgttt ctacctaatg ccatttctcc taattccata gatggtccat ctgctaaaat
    17341 ctcgttatac tcaacaacat ctccaactgc aacgattgga cgttggttgt aacatgtacc
    17401 tgagtttgaa cgtttaaatt tagctaatgg atagcgatct aattcacctt catgctcaac
    17461 gccgttctct tcaactagac gacgaacaag aatttcatta gattcaacat gttcaacacg
    17521 acctctgtgc ttagctgtaa tagccgcacc agaatcacgt gctgcaacgt gttccatacc
    17581 tgtaccaaca aatggtgctt ctggattcat caaaggcact gcttgacgtt gcatgttcgc
    17641 acccatcaat gcacggtttg agtcatcatt ttctaagaat ggaatacatg ctgtcgctgc
    17701 tgaaacaact tgcttcggcg atacatccat ataatccatt ttttctttag ccataactgt
    17761 attgttacca cggaaacgac atacaacttc atcatccatg aaacgaccat tttcatctaa
    17821 tttagagttt gcttgtgcta caacatagct atcttcttcg tcagctgtta aatagtcaat
    17881 ttgatcagtg atagcatgtg tatctaaatc aactttacga tatggtgttt caataaagcc
    17941 gaattcattt acacgtgcat aacttgataa tgagttaatc aatccaatgt ttggtccctc
    18001 aggtgtttca attggacaca tacggccata gtgagagtag tgaacgtcac gtacttccat
    18061 ttgagcacgt tcacgtgtta aaccaccagg tcctaatgct gatagacgac gtttatgcgt
    18121 taactcagct aatgggtttg cttggtccat gaattgtgat aattgagagc taccaaagaa
    18181 ttctttaata gatgcaataa caggtcgaat attaattaat tgttgaggtg tgatagactc
    18241 agtatcttga attgacattc tttcacgtac aactctttcc attcttgata aaccgatacg
    18301 gaattggttt tgtagtaatt cacctacaga acgtaaacga cggttaccta aatggtcaat
    18361 atcatctgta tatccaatac cgcttaataa gttaaagaag taactcattg aagcaatgat
    18421 atctgctggt gtaatgcatt taacttctga gtcagggaaa gcattaccaa ttacagttgt
    18481 cgtacgacct tcatcatcgt taggaacata tactttaatt gattgaattt ctactggctc
    18541 gtctataacg ctaccatgca attcaaacac ttcgctgttt gcatttgatt caagtacatc
    18601 catgatttcg tcgattttac gacgatcaag cactgtacct tcttcaacta caatttcacc
    18661 agtttcagta tttacaattg gctcagctaa tttttgatta aataaacgat gttttaaatg
    18721 taattttttg tttgttttat aacgacccac gcttgctaag tcatagcgtt ttggatcaaa
    18781 gaaacgtgaa tacaatagac ttttagcatt ttcaacagtt ggtggttcac ctggacgtaa
    18841 acgttcatag atttctaata acgcttgttc agtgttttca gtgccgtctt tctctaaagt
    18901 attacgtaaa tattcattgt cacctaaaag gtcaacaatt tcttggtcgc ttgagaaacc
    18961 taatgcacgt aacaatactg ttaatggtag tttacgtgtt ctatcaatac gtacgtatac
    19021 aacatcttta gcatctgttt catattctaa ccatgcacca cggtttggaa taattgttgc
    19081 atcatagttt tcacgaccat ttttgtcgat tttttcattg aaataaacgg atggtgaacg
    19141 aactaattga gatacgatta cacgttctgc accattgata acgaacgtac ctgtatcagt
    19201 cattaatggg aaatcaccca taaagacttc ttgttcttta acttctcctg tttctttaat
    19261 gattagacgc acttttacac gaagaggtgc agcataagta gcgtcacggt ttttagattc
    19321 ttctaaatca tattttggtt ctcctaaacg gtaatccaca aactctaatg acaaattacc
    19381 agtaaaatct tcaattggag aaatgtctct aaacatttcg attaaacctt ctcttaggaa
    19441 ccactcgtaa gatttagttt gaatttctat taagtttggt aattctaata cttctgaaat
    19501 tctcgcgtag tttctacgtt tacgatgtct tccatattgg acaacttgac ctgccaaaca
    19561 gattcacccc tcaaaaatta tgtgatccgc ttttagctaa ataccataag catattaata
    19621 aaaagacaaa aagaaaacgg caacactaat gatgacacca ttttcgattc tatatttatt
    19681 cttaattaac ttatctattt aacgtaaaag tacacactta ttgaacataa atttttacat
    19741 tatataacta taacagaata tccatttcat ttcaagcttt tatacttctc agaatgtaat
    19801 atcctttatc tttatttacc acttctacat ttccaaaaag ttcattcatt cttttctttg
    19861 cagatggcat accttgcttc ttctgaatta caacgaatag ttcaccgttc gagtctaatc
    19921 tatgtaatgc ttgctcgaat atacgatgca cggtttcttt ccctgctctt attggtggat
    19981 tggttaaaat aaaatcaaaa cttttgtctt ccacagcaga caaagcatca ctttccttta
    20041 cgatcacatt atcaatacca tttaattttt tgtttttttc aactaaggct agcgctctgt
    20101 gattaacatc tagcattgta attgaatgat gtggtgatac tttagcaatc atcaaaccaa
    20161 ttggtccgta accacaacca acatcggcaa ttcgcttact tggaccaggt ggatgcgctt
    20221 ttaaaaaagt ttgaacaaga acatctgaac cataatctac tttatctttc gaaaacactc
    20281 cgttatcagt tattaaatca attttatgat ggtttaattg atattgaata cgttgttcat
    20341 tgctaattac acttggatct tcatcgtaat aatgactcat tattccacct catcttcact
    20401 ttgtaactcg tcattataat tataacattt cactcagtct ttaaatgaat aaaaccccgt
    20461 tatcaaaata acggggtttt aacttctcaa agaatcatta tattatagca atacttgcta
    20521 taaaactttg attattaagt ttaagatact tgaattattt taattctaca gtagctccaa
    20581 cttcttctaa ttgttcttta agtttttcag cttcttcttt aggtaaagct tctttgatta
    20641 ctttaggagc tccgtctact aattctttag catcttttaa tcctaaacca gttgcttctt
    20701 taacagcttt aacaactttg attttagatg aaccagctga agttaactca acgtcaaatt
    20761 cagttttttc tgctgcagcg tcagcgccac cagctgcacc tgctactgct actggagcag
    20821 ctgcagttac accaaattct tcttcaattg cttttactaa gtcgtttaat tctaatactg
    20881 acatttcttt aatcgcttca atgatttgtt catgattagc cattttataa ttcctccatt
    20941 atttttaatt tttttacgct aaaaattatt cagcgttttc ttctttttgt tctccaatag
    21001 ctttaaccgc ataagcgaag ttgcgtacag gagcttgtaa tactgataaa agcatagata
    21061 caagaccatc gtgtgaaggt aatgaaccaa cagttttaac ttcttctgct gtaataacat
    21121 tgccttccat aacgcctgat ttaatttcta atgcttcatg atctttagca aatccagaaa
    21181 ttactttcgc tgcagctaca gcatcttcac ttgaagttgc aatagcagta ggacctgtta
    21241 agaattcatc taagccttcg ataccagctt tttcagctgc acgacgtacc atagtgtttt
    21301 tgtatacttt atactcaaca ccagcttcac gtaattgtga acgtaagtca gtaacttcag
    21361 ctactgttaa tccacggtag tcaacgatta ctgttgaaac tgaatttgat agtacctcag
    21421 caatttcatc aactagttgt tttttagctt caatgatagc agacattcag acacctccat
    21481 ttaaattttt ggtgcttttt ttaaaattaa taaaaaaagc actttctacc cacggcaaaa
    21541 agtgcttgaa agatcataga tcatcacgtt caagtcaatt ttagcctcgg caggataaat
    21601 tttaagttat aaataactcc tactgtctta ggtaaaataa tcacaataag taatataact
    21661 tggaaattac ttattgtcaa tatattttta cagactgaaa gatatttctt tcagcctgta
    21721 attgtttata tcatttatta tttgaaactt gcagtatcaa ttttaactcc aggacccatt
    21781 gttgtagtta cagcaacaga tttgaagtat gtacctttag cagatgatgg tttagcttta
    21841 gctaatacat cttgtaaagt attgaagttt tcaattaatt gttcatcagt aaatgaaact
    21901 ttaccaattg atgcatgtac gataccagct ttttcagcac gatattctac tttaccagct
    21961 ttgatttctt caacagcttt tttaacatcc attgttacag ttccagtttt agggtttggc
    22021 attaaacctt ttggtcctaa tacacgacct aatttaccaa cttcacccat catgtctggt
    22081 gtagcaacta ctacatcgaa gtcgaaccaa ccttgttgga ttttttgaac gtattctgct
    22141 tcacctacat agtcagcacc tgctgcttca gcttcagcaa ttttgtcacc tttagcgaat
    22201 actaatacac tttgtgattt accagttccg tttggtaata caactgcacc acggatttgt
    22261 tggtcatttt tacgtgtatc aattcctaaa cggaatgcaa cttcaacaga agcgtcaaag
    22321 ttagcaatgc ttgtttcttt agctaattta attgcttctt caacactgta gtgctgagta
    22381 cggtcaactt tactagctgc ttcttgatac tttttacctt ttttagccat ttatagttcc
    22441 tcctttagtg gttttagcgg aatttcctcc cacattactt gctatatgca agttaagagc
    22501 agacaacaga agaattaatc ttatgaataa atcaatcatt aatctagtca ttcagattat
    22561 atttataatt catcattatt atctctgtta tctgcttcat cgtttaaatc atgtaatcaa
    22621 atttaatatt cgtaaaatta ttctacaacg atacccatac tacgtgcagt accttcgata
    22681 atacgcatag ctgcttcttc gtcagcagcg tttaagtctt gcattttgct gttagcaatt
    22741 tcgcgtactt gatctttagt tactgtagca actttagttt tgtttggttc gcctgaacct
    22801 ttttcaatac cagctgcttt tttaagtaat actggagccg gtggagtttt tgtaataaat
    22861 gtaaatgaac gatcttcata aacactgatt tctaccggaa taattaaacc tgcttgatct
    22921 tgagtacgtg cattgaactc tttacagaat cccatgatgt tcacacctgc ttgacctaat
    22981 gctggaccaa ctggtggtgc tggattcgct ttacctgcag gaatttgtaa tttaacaact
    23041 ttatctactt ttttagccac gatgtgcacc tccttgatat cgtgatgtgg tcacagggct
    23101 cagttttgcc ctcccactct taacatttcg tgacgaaatg ggcgctctaa aagcgcgacc
    23161 acataatttt aacacttttc taattaaaaa atcaatactc ttttttatct tttggttagt
    23221 ttaataactt taattgttaa ttaaagcttt tctatttgat cgaattcaac ttctactggt
    23281 gtttctcggc caaacatatc tactaatact gttagcttaa acttatctgt ttcaatttct
    23341 tgaacttcac caacttgatt cgcaaatgga cctgatttaa tacgaacttg ctcgccaact
    23401 tcgagttcaa catcgatagt cttttcttta agacccattt gttttaagat gaagcgaact
    23461 tcttctggta acaatggatt tggcttagac cctgcacctg cagaacctac aaaaccagta
    23521 acgcctggtg tatttcttac cacataccat gattcatctg tcatgattaa ttccactaaa
    23581 acatatccag ggaatgtttt tttaacagtc gttttagctt tgccatcttt tacttgagtt
    23641 tcttcttctt ccggtatgac tactctaaag atttgttcag tcatattcat agattctact
    23701 cttttttcta aattcttttt aactttattt tcatatccag aatatgtatg cactgcatac
    23761 caacgctttg cgccaacttc ttcagacatg tcttcactcc tctaacgaaa taataaattt
    23821 ttcaatgctg taattcctaa atctaaggca tagaaaaaga ctaagaagaa tataacagta
    23881 gaaactacaa ttacagtata tttaaatagc tcttctttcg tcggccaact tgttttttcc
    23941 atttcagact taacgccttt aaagaaactt tcttttttag ccatctgctc aacctccaaa
    24001 tcctttgaac atattatcaa agtattattt cgaatgtatt tatttcgatt ctttatgaat
    24061 tgtgtgcgcg ttacattttg gacaatattt ctttaaggtt aatcttgttg ccgagccttc
    24121 ttgcttagga acattataat ttctattgcc acaagcttca caatttaaag gtatttttct
    24181 cacaataatt cacctttatt cattataata ccagtatact atacataaaa tttataacaa
    24241 atgtcaaacc atcagtaagt aacacgttat aattcaattc acgtcctaag gcgcttttca
    24301 aatcattttg aaataacgtt ttattttatt tttacatcgt tgtatggcat tataaatcac
    24361 tttctctttt acatgcatca attgagcaat ttctctcggc ttatattgtt cacacattaa
    24421 atacatgaca tctttttcaa atgcactcaa atacttaaac tgacaaatca actctttaat
    24481 tgctaattta ttcaaataat tgttttcaac atcaaaacgc tttattgcac atggatacgt
    24541 aactctatat tcattaatca aattatccat tcttttattt gtagctaaat attttctcaa
    24601 ataatcatac tttacagacg taattaaaca ttgaacataa tttgtgaaag gctgctctcc
    24661 actaaaatca aatgtttgga ccgacctata catccgtata agtatttctt gatataagtc
    24721 ttctaaatca taatgattaa atccaaatgt tttgatgcgt cgaataatta atggttgtag
    24781 atccattact aacttttcga agtctttatc atttattgca ttgttacgtt tgattgtact
    24841 gtcttgagtt gtcaaatcgt atttcaaata ttaaactaac cccttctatc taaaatttaa
    24901 ggttagttta atattgttac attcaaaatt tcaagatgac ggaaatgtca tttcttatga
    24961 tgtcctcttc gtattttttc aaattctgca aggatttcag aagataacgg aattcgagtt
    25021 cttggcttgt tttcacttat atcatctaat gatttactca catcaatttc attttctttt
    25081 aaatctctcc acatttcgcg agatgatatt ctatatgcac ctgatccaaa gatagcatgt
    25141 tgctcactca tatcacttgt tacaactgta atatgcttag tatgcttgtc ataaagttca
    25201 taaacataac gttctatgaa actatcagct gtttcttttt ccttggtaaa aatcgtttta
    25261 acgccatgat acatgtattc tctttcaaca cccgattggt cataagcatc gaaaacacaa
    25321 ataatttcat ctgaaataac tgcattataa tttgcaattg catctattaa ttgcattcta
    25381 gcttcttcta aattctcttt tgcaatggcg cttagcgttg gtgattgtcc tatcatattg
    25441 tatccatcaa tgattaagta acgttctttc attatatttc tccaacatca tgtctttttc
    25501 gaaatacttc gtacatcatt aaacttgctg caaccgaagc attcaaactg tttacatgtc
    25561 caaccattgg aatcttaata taaaaatcgc atttatcact tactaggcga ctcataccct
    25621 gtccttcgct accaattaca atagccaatg acatgtccgc ttctagattt ctataatctg
    25681 ttgcattatt agcttcagtg ccagctaccc aaaagccatt atcttttagt tcatcgatag
    25741 ttttagctaa atttgtcact cgaataactg gtacatgttc aattgcacct gttgaggctt
    25801 ttgcaactgt ttgcgttagt gtaactgaac gacgtttagg aataataaca ccatcaactc
    25861 ccgttgcatc ggctgttctt aaaattgatc ccaagttatg tgggtcttct aagccgtcta
    25921 atataagtac tgtcaataaa ccttcttttt ctttttgctg ttttaaaaat tgatcgaagt
    25981 cagcatattc atatggtgca ataagcgctg caacaccctg atgtggtgca tttgctaaaa
    26041 aatctaattt agattttggt acagtttgaa caatgatttt ttgatctttt gcatttttta
    26101 aaatttcatt aatttgttgc tttttaatac cttcttgaat caatatctta tttatcggat
    26161 gcccagtaat aatcgcttct ctaacagcat gcctaccaac aataaccgta tcttccacat
    26221 cgtcactacc tttcgtttac tattgttatt attttattta ataatgcctc taatcgttct
    26281 tctctttttt ctaaataaag aaaacctatc actgcttcta tcgctgaact tttacgatat
    26341 gtttgaacat cagtgttttt agctttagta tgacttttcg cgttacgccc tcgcttcaaa
    26401 atatccattt cttcgtctgt aaaccattct tgctccatta aatattctaa cgtttgcgcc
    26461 tgacttttgg cagatacata ttttttagac atttgatgta gtttattagg cttactttta
    26521 agctttaaaa cgatataggt acgtacatat tgatctaaga ctgcgtctcc catatatgct
    26581 aaggtcaatg gattcaataa tttaatgtga ttatcttgtt gattatccac gtttaaatct
    26641 aaccccttga ggtgtgtctt ctaatataat gttttgtgat ttcagcatgt ctcgaatttc
    26701 atctgctcgt gcaaaatctt tgtttttcct tgcttcatta cgctcttcga ttaatttttc
    26761 aacatcttca tccaataatt catctgcatt tttagatttt aacggtacac ctaaaacatc
    26821 gctgaaaatt tgataaactg ctttaaattt atcaattact tctgttgatg ttgtgttctc
    26881 tagtacatat ttattcgcaa gttttgctaa atcataccaa gctgtaattg cattagctgt
    26941 attaaaatca tcattcataa ctgtttcaaa acgatttaaa atcgcatcaa tttgatcaat
    27001 atatgtctgt tgattttcaa tattagtagc aatttgtgcg cgctcttcaa ttaattgata
    27061 actattgcga atacgctcta gtccactacg tgctgattct accaattcta gattatagtt
    27121 aattgggctt ctataatgta cgctaatcat aaagaatctt agtacatctg gatcaacttc
    27181 tttaataata tcgtgaacta aaataaagtt gcctagtgat ttactcattt tttcattatc
    27241 aatattaatg aaaccattat gcatccaata attagcaaat ggcgcatgat tatgtgcttc
    27301 tgattgtgct atttcatttt catgatgtgg aaattgtaaa tctgaaccac ccgcatgtat
    27361 atcaattgta ggtcctagct catgaaatgc cattacagaa cattctatat gccatcctgg
    27421 tctaccttca ccaaatgggc tatcccaact aatctcgcca ggcttcgctt ttttccacaa
    27481 tgtaaaatca agtgcatctt ctttatgctc tcctgcatct atacgagcac ccacttttaa
    27541 gtcatctatg gattgatgac ttaatttacc ataaccttca aatttacgtg ttctaaagta
    27601 aacatcgcca ccactttcat atgcataacc ttgatccacc aaatctttaa taaattgaat
    27661 aatgtcatcc atatggtcca ttacccttgg atttgaagtc gcttttctaa catttaacgc
    27721 accaacatct tcatgaaaag cagcgatata tttttctgca atttcgggaa cagactgatt
    27781 taattcttga gaacgtttaa ttaatttatc atctacgtct gtaaaatttg atacatattc
    27841 tacattatat ccttggtatt caaagtaacg tctcactacg tcataattaa ttgctggtct
    27901 tgcgttacca atatgaatgt agttatatac agtaggacca catacataca tttttacttt
    27961 ccctggttct ataggcttga acacttcttt ttgacgtgta agcgtattat ataatgtaat
    28021 catcttgaat ctctccattc ctagtctttt caagttgtcg ttctaaatgc ttaatttgtt
    28081 cataaattgg atcaggtaga tggcgatgat caaatgtttt tccaactcga acaccatctt
    28141 gcttaacaat atgtcctggt ataccaacaa ccgttgaata acttggaact gattgtaaaa
    28201 caactgaatt tgcaccaata tttacatttg aatttatttt aatatttcct aaaactttcg
    28261 caccggctgc tattaaaaca ttgtctccta tatctgggtg tcttttccct ctttctttcc
    28321 ctgtcccacc aagtgtcacg ccttgataga ttgtcacatt atcaccaatt gtacatgttt
    28381 ctcctattac aacgcccata ccatgatcta taaatagacg ctttccaatt ttagcacctg
    28441 gatggatttc tatacctgtg aaaaatcttg aaatttgaga tatcgcgcgt gctgcaacat
    28501 attttttttg gttgtataac ttatgtgcaa tcaaatgact ccaaactgca tgtaaacctg
    28561 catacgttgt aatgacttct aatgttgaac gtgccgctgg atcctgctca aataccattt
    28621 ttatatcgtc tctcattctt tttaacaaga tcatttcctc ctcaatgatt gaactacgta
    28681 aatacataat tgaagtacct gcgaaattaa atatcaaaaa agcaccacta acatacaagt
    28741 tgtattgtta gaggcgcttc cgcacggttc cactctgaat ttagcgaata acattaataa
    28801 tattgcgggc gcttccaaat tatcaaggaa actaagtcaa cttaatgctc atcactctca
    28861 ttatatattt aattcatttt acgaaggtgc attcattaat ttctacgttg tactcacagc
    28921 aaccgtacac tctctgcatc gtataaattt aattactaat ccttcgtttt atataaaata
    28981 ctttattcat attgttgtaa tttgttaata caactttatc atatgtggtt acgttataaa
    29041 attcaagtat atactacctt gatcttgtct atttcattac ttatattgtt ttaaacggtt
    29101 tagcactttt tctttaccaa gtacttcaat tgtatttggt aattcaggac catgcatttg
    29161 gcctgttaca gcaacacgaa taggcataaa taattgcttg ccttttattc ctgtttcttt
    29221 ttgaacttct ttaattgtct ttttaatttc agccgcttca aatggttcaa gtgcttctaa
    29281 tttactgaat aagtgcgtca ttaactctgg tacttgctct ccattaatca cttgttgttc
    29341 ttcttcacca agagctggca tttctttaaa gaacatttct gataaaggta caatttcacc
    29401 ggcataactc atttcttttt gataaagcgc aattaatttg cgtccccaag ataaatcctc
    29461 ttctgacggc acctcaggaa tcaaatttgc tttaattaaa tgaggtaatg ctaattggaa
    29521 tactgtttca gtatcttttt gtttcatata ttggttatta acccatgcta atttttgctt
    29581 atcgaaaaat gctggtgatt ttgacaaacg cttttcatca aagattttga taaattcttc
    29641 tttagaaaag atttcttctt caccttcagg agaccaacct aataacgcaa taaaattaaa
    29701 taacgcttca ggtaaataac ctaagtcacg atattgctca ataaattgta aaatttgccc
    29761 atcacgttta cttaactttt tacgttcttc attaacaatt aatgacatat gaccaaaacg
    29821 aggtggctcc cagccaaatg cttcataaat cataatttgt ttaggcgtgt ttgaaatatg
    29881 atcatcacca cgaattacat ctgaaatttg catgtaatga tcatctatag ctactgcaaa
    29941 attgtacgtt ggaatgccat ctttttttac gataacccag tcaccaatac catttgaatc
    30001 aaatgaaata tttcctttta ccatatcatc aaatgaatac gtttggtttt gaggtactcg
    30061 gaaacgaatt gatggttggc gtccttctgc ttcaaattgt tgacgttgtt cttcagtcaa
    30121 atgcgcatgt tgaccaccat agcgaggcat ttcaccacga gcgatttgcg cttcacgttc
    30181 agcttctaat tcttcttctg tcatatagca tttatatgct ttatcttctg ctagtaactg
    30241 atctattaat ggttggtaga tatgttgacg ttcagattga cgatatggtc cgtagccatt
    30301 gtctttatct acagactcat cccaatctaa tcctaaccat ttaagattat caaattgtga
    30361 tgtttctcca tcttctaaat tacgtttttt atcagtatct tcaattcgaa tcacaaaatc
    30421 tccgttgtaa tgtttagcat acaagtaatt gaataatgct gttcttgcat taccaatatg
    30481 aagataccca gttggacttg gtgcatatct tactcttata cgatcgctca tttttttcac
    30541 tcctaaatta aatatcagat tttcaagtta gttcatataa attgttcatt tgctatcttc
    30601 gaccgtcata acaaatgtct aactcgtctt attgttaaaa cgaaacaatg ctttttaaca
    30661 tgaccttaaa ataatttcat tgtttaatca taacataatt ccctgggtaa tatgcttaaa
    30721 ttttaaatag aaagctgttg ttttttcaac actttaaaaa agctatccct aagaatcaag
    30781 actgcactac ataaacattt caacgactta aaaatgaata ttatgtactg tgaaattaaa
    30841 cttcaaatta actattcaaa tacgttaaaa ttgattctaa ttttgtatgt cttgattgct
    30901 ataagaataa ctttattaat atctaaaatt taacacttaa tgaacttgtt tcaatgatat
    30961 attagcacta tttgtatttt ttgataacta atatgttttg catttattta tagttatact
    31021 tcaaattaca aacttcgcca tttcatatac cttttaatat ctattttgtt ttcgtcaact
    31081 acagttttta taatgatact gtatcttcga tttttttagc aaaaacaatt cttcctgaag
    31141 atgtttgcaa taagctgact acttctaaat tgacatgact gccaataaga tttttagcat
    31201 tatcaacaac taccatcgta ccatcatcta gatatcctac tgcctgacca ggctccttac
    31261 ccatttttgt cagtaaaata tgcagttgat caccttgatg tacattaggt ttgattgctt
    31321 ctgataaatc attaacattt aatgctttga taccatgtac atgacaaact ttatttaggt
    31381 tgaaatctgt cgttataata cttgcatgat attgttttgc taattttaat aacatcgtat
    31441 caatatcact atgtgtttta gttggatgta taacctttgt aggatagtct aaatcataca
    31501 attcatttaa aatatctaag cctcttttac ccttttcacg tttaacactg tcatttgaat
    31561 ctgcaacaat ttgtaattca ttaataacac cttgtggaat taaaatattg ccatcgataa
    31621 aaccgcaacg aatgacttct aaaatacgac catcaataat tgcgcttgtg tcgataattt
    31681 ttggcgtagc acttttagta tgttgtgaca tggaacgcgc tatattctca ggtaaaaaca
    31741 ttaacatttc atctcgtttt ttaaggccaa attggaaacc gaaataacat agtaatatcg
    31801 taattatgac aggaatgaaa tgattaaaaa tagagttgcc aattgattct aatataaacg
    31861 acaccataac agaaataagt aatccgatta ttaaacctat tgttgcgaat agtatttcaa
    31921 cagcacttct acgcataata aaatgttcta aaccttttat agcgttagta actcgtctaa
    31981 taaatacacc aaaaattaag aacataaaaa tactaccgat aatgccatct acatagtgat
    32041 tttttaaaaa gctggagttt tgtaatccaa gatcatttgc aatttcagga ataataatta
    32101 ttcctaatgc gctcccaata attaagtaaa taataataac cattagttta acgatattca
    32161 cacaatgtcc tcctttcttg atgttttatg aatgaagagc aaatgacaat acttcatgta
    32221 cagtagttac acctattact tgtatacctt caggatatgt ccatccgcct atattatttt
    32281 taggaataat tacacgtttg aaacctagtt ttgcagcctc ttgcacgcgt tgttctatcc
    32341 gagatacacg acgtacctca cccgttaaac caacttctcc aatatagcaa tctaatccgt
    32401 cgacagcttt atctttaaag ctagatgcag ttgctacaat tacacttaaa tcaactgctg
    32461 gctccgttaa ctttacaccg ccagctactt tgatataagc atcttgttgt tgtaatagat
    32521 aattttcttt cttttccaaa acagccatca acaaacttaa tcgattatga tcaatccctg
    32581 ttgccattcg tctcggattg ttaaaagtcg ttggagttac cagcgcttga acttctatta
    32641 aaagtggtct ggttccctcc atggttgcaa caattgttga acctggaaca tttgttgaac
    32701 gttcttctaa aaacatttca gatggattat ttacaccttt taatccactt tgcttcattt
    32761 cgaagattcc catttcattc gttgaaccaa aacggttttt aacagctcgc aaaattcgat
    32821 atgcgtggtg ttcatcgcct tcaaaataaa gcacagtatc aaccatgtgt tctagcaatc
    32881 ttgggccagc aatttgacct tctttcgtta catgacccac tataaaagtt gcaatgttca
    32941 tttgtttagc aatattcatt aaactttgtg tactttcacg aacttgtgaa acagaacctg
    33001 gcgcagagct gatttcagga tgatatattg tttgaatcga atccactact aataaatcag
    33061 gttgttcttc ttttactgtt tgataaataa cttcaagatc tgtttcagct aatacttgca
    33121 attcacttga atcttcatct aatcgctctg cacgtaattt agtctgacta agcgattctt
    33181 ctccagtaat atatagtact tttttctttt gagataacga tgcacaaatt tgtaaaagta
    33241 acgttgactt accaatacct ggatccccac caataagtac taacgatccg ctcacaatac
    33301 ctccacctaa tacacggttg aattctgctg aatctgttaa cactctcggc gttgtttcat
    33361 gtttaatact atttaatttt tgtactttac ctgctaattc cttggtttta actccatgtt
    33421 taggattggc tgctttttca acaatttcct ccatttgatt ccaagcgcca caattaggac
    33481 atttccccat ccatttagga gattgataac cacaagccat acattcaaaa atcacttttt
    33541 tcttggccaa aattgcacct ccactttcct ttgaacaaat ctattttaaa cgcacattcc
    33601 cattatgaca aattaaatgt gagttacatt tgtttgtatt ttaacatgac tactaacgca
    33661 aactgtttta ccagatttat tttttatgaa tattataaaa taaacttaca tcatgttgat
    33721 agtcggtatc tttcaaagat aaaatcttaa taatttctta gtaaactctt ttctctagat
    33781 ttatcacaat attatataga cctattttat tttgacgtaa gttgctagta tcttcaaaca
    33841 aaaaaccttt ataaaaattc atacctttat gctatcgctg taggctcatt aacttgttac
    33901 atataattct taactatcct ttgatgattg ttttattaga ttgtttcgtt gatggatact
    33961 ttcacgaatt tctatagttc aatgctacta aaaaaacagc ccaaaacttt aatttgtttt
    34021 ggactgtttt ataattatgc ttgcgatggt gttttagttt ctgaagtttg ttcagcaatg
    34081 tcatatttaa actctttacc atcatgatct actgtaactt tcttaccttc aatttgatta
    34141 ccatctaata ttaattcact taaattatct tcgatagttt tttgtatcgc tctaattaat
    34201 ggtcttgcac catattctgg atcatatcct tcttctgcga ttttgtcttt cgctttatca
    34261 gttacaataa tatttatgtt ttgttcagat aatcgatttg ttaatttatt aaccatcatt
    34321 gttacaattt cttttaattc ttcttttgtt agtttatgga atacaatgat atcatctaca
    34381 cggtttaaaa attctggacg gaatgaattt tttaattctt ttaacatcgt ttttcgaatt
    34441 gtttcataat cttgtccatc acttgaacca ccgaatccag caaatcgttg atcttgtaat
    34501 tcttgtgccc caacgtttga tgtcattatg ataattgtat ttctgaaatc aactgtacgt
    34561 ccttttgtat ctgtcaaatg tccatcatct aaaacttgta atagaatatt aaatacatct
    34621 ggatgagctt tttcaatttc atcaaataaa attacagaat atggtttacg tctaactttt
    34681 tcagttaatt gtccaccatc atcatgacca acatatcctg gaggagcacc aactaatcgg
    34741 ctcactgcgt gtttttccat aaattcactc atgtctacac ggatcatcgc atcatcatcg
    34801 ccaaacattg attcagctaa agctctagct aattcagttt taccaacacc agttggtcca
    34861 aggaagataa agctaccaat tggtcgttta ggatctttta accctgcacg ggcacgtcta
    34921 accgctttac tgattgaatt aacagcatct ttttgcccaa taactctctc atgtaatgta
    34981 tcttctagac taagaagttt ttcagattct gtttcattga ttttagttaa tgggatacct
    35041 gtccatcctg caataacttc agcaatatct tcttctgaca atgaagttga catgccattt
    35101 tgtgcattct tccattcatt tttagcttct tcatattgct tttcaagttt tgtttgttta
    35161 tcacgcaggt tagcagcatt ttcaaactct tgagcatgta ctgcggcatc tttttcattt
    35221 ttaacttttt caatttcttg ttcaatttct tttaaattat taggtgtcgt atgactctta
    35281 agtcttactt tagaacttgc ttcatcaatt aaatcaattg ctttatctgg taagaaacga
    35341 tctgaaacgt atctgttact taatttaaca gctgtttcaa tagcttcgtc tgaaatatta
    35401 atacgatggt gtgcttcgta acgatctctt aatcctttta aaatagcaac tgtatctact
    35461 actgaaggtt catcaacttg tacaggttgg aaacgacgtt ctaaagccgc gtctttttca
    35521 atatttttgc gatattcatc taatgtagta gcaccaatac attgtaattc accacgtgct
    35581 aatgccggct tcaaaatatt cgaagcatcg atagcacctt cagcaccacc agcaccaact
    35641 aaagtatgca actcatcaat aaataggatg acattacctg cttgttggat ttcttccata
    35701 acctttttca gacgctcttc aaattcacca cgatatttag tacctgcaac tactgttccc
    35761 atatctaaag acataacacg cttatctttt aatgtctctg gtacctcatt attcactatg
    35821 gcttgcgcta aaccttcagc aatagcagtt ttaccaacac ctggctctcc aataagcaca
    35881 ggattgtttt tcgtacgtct acttaatact tcaattacac gtgtaatttc tttatcacgt
    35941 cctataacag gatctaatgt accgtctttg gcaatgactg ttaagtcacg agctaaacta
    36001 tctaaagttg gagtattatt tgacttacta gcttgtgcat ttttattact catttcaggg
    36061 tttcctaaag ctttcacaac ttgtgcacgt gctttagtaa tatttaaatc tagatttgca
    36121 aaaactcttg ctgcaacacc ttcattttca cgaatcaagc ctaataaaat atgttccgtt
    36181 ccaacaaaat tgtgatgtaa ttttctagct tcatccatcg ataattcaat gactttttta
    36241 gctctaggtg tataatgcaa tgtaccaaca tgatcttgac catgtccgat taatttttca
    36301 acttcttcaa ttactttatc ttcagtgata ttaaaacttt ctaatacttt tgcagcaatt
    36361 ccttcaggtt ctttcattaa ccccaataat aggtgttctg ttcctatatt tgaatgattt
    36421 aaacgaattg cttcttcttg ggcatgtgct aatacgcgct gtgcacgctc agttaatcta
    36481 ccaaataaca taaataatga cctcctactt tatatgttct cttagtatat ctgctcgttt
    36541 ttcttttaca gatttgtcat cttcttcatc taataaaaat ggtgactgta tagctaccat
    36601 caattcatta aatttaaagt tttgtaattc aatgtaattt aaatctatac caagtttaac
    36661 ttcgcttaat ctataagaag cctcttccat agttatcatt ctacagtttt gtaaaatacc
    36721 tagcgagcga aaaacacggt cttgtgtttc taattgatta taagtgtcta acttttgtcg
    36781 tatttgtttt tcttcatgaa tgatttgatt aacaacttct gttaatgttt ctatgatttc
    36841 taactcagat ttaccaagtg taagttggtt ggatacttga taagtatgtc cataaacttg
    36901 cgaaccttca ccgtaaatac ctctgattgt atatccaaaa cgattaatgg tttgagcaat
    36961 ccgtgtcatt cttttcataa tagatagacc tggcaaatgt agcatcacgc ttgctctcat
    37021 accagtacct atattggtag gacatgtagt taaataacca agttgttcat cataacttat
    37081 atcaaggctt cgatctaatt catcatcaat tgatgaagct tgattatata aagcctgtaa
    37141 tgtcgtgtca gttcccatag cttgaatacg aatatggtcc tcttcattta tcatgacact
    37201 taaagattca tcgtcattca ctaatactgc ggctgctggt tgttttatta gttcaggact
    37261 aatcaaatgc tttgcaacca ttttcatttt actttgttga tccatttgat caagacgcat
    37321 taattcaaag tttggcaagg catcttgtac ctcatttata actctaaatc catcattttc
    37381 agtagcatac attagtggat gcacatgatt ttctaaatta cgcgctaacc gaattctaga
    37441 agacataaca attggtgttt cttcattact tttcatccat tggctgatat tatcatgaat
    37501 attatgcgtc atcatgttgc acctcactct cagcttttag tgctttaatt tcatctctaa
    37561 caatggctgc ttcctcaaaa tcttggattt caataagttt tttcaaatat tcattctttt
    37621 cttcgatttt tcgctttaaa gctatctttt tatgtgaaga atgtggtgtc tttccaacgt
    37681 gctcaaattg tccaccttga actctgcgga cgatatcaat gatgtcatct ttaaatgttg
    37741 cataacaatt agcacaccca aatttaccaa catgtgcaat atctttcaaa gtcatatgac
    37801 atgatgggca acgtttttct tctttaaacg ctacttcttg aaaattaatt ccatgtttcg
    37861 tagctaaatg ttgtaaaatt tgcttaacaa caaatgcttc ttcgaaatta tcttgatgtt
    37921 cttgatattc aggttgttca ttagcttgat tccacggatg gtgtccctca gcacaagttt
    37981 gacacaccat tttttcttct gttttatttt tacttgtaac tttaactttt aattccgctt
    38041 cattaagttg acaattttca caaagcactt caacacctca tttcatttag taataattta
    38101 taactggtaa caaacgtttt aaaatatttg ctctaataat atctctagaa accatatcca
    38161 ttgatagcgt ttctctatca ataactgctt gaatcatttt agcttcacgt tcatttatta
    38221 acattttatc taaaagccca tcaataatat aataagcttg ttgttgagaa atagaaggtc
    38281 caatcagctg aagcaaatga ttaatataac ctgttgcatc tttattttca attttagtga
    38341 ttcggatgta accaccacca ccacgtttac tttcgatttc ataaccatgt tcattagtga
    38401 atcgtgtttt gattacataa tttaattgtg atggtacgca atcaaaacgc tgtgcgatat
    38461 tcgctctctg aatttcaacg acatcttcat tcgactcttc aaataaacgt ttgatgtatt
    38521 gttctatgat gtcagacata ttgtgcatgt atatcacccc tttttgacct tctttgacta
    38581 tattatatga cctattttga cctttttcaa ttaatttgtt tttatatttt caaaaattta
    38641 tgatgttagc ccttccattt tattgtatga tagattgtag aaaaaactaa aggaatgaga
    38701 aatatatgca tattctaatt gggattatcg gaattatctt ctttttagca cttgcgtttc
    38761 tatttagctc agacaggaaa aatattcgct ggaaatatgt aggaatttta cttgcaattc
    38821 agcttatctt tgcatttatc ttgcttaaaa ctacaatggg aatcaaggtt attggtggta
    38881 tttcaaacgg ttttaactac ttattattaa aagcagcaga tggtgttaac tttgtattcg
    38941 gtggtattca atatatcgat cctaagcaac caccattctt cttcagtgta ttattaccta
    39001 tcgtatttat ttcagcgatt atcggtatcc ttcaatacac aaagatttta cctttaatca
    39061 ttaatgtttt aggattctta atttctaaaa ttaatggtat gggacgttta gaatcataca
    39121 atgcagttgc tgcagcaatt ttgggacaat ctgaagtatt tatttcaatt aaaaaacaat
    39181 taccatacat acctaagcaa cgtttataca cattaacagc ttcagcaatg tcgactgttt
    39241 ctgcttcaat tatcggtgca tactttacct taattgaacc taaatatgtt gtaacggctg
    39301 ttgtcttaaa cttatttggt ggtttcatca tcgcttcaat tattaaccct tataaagtta
    39361 atgaagaaga tgacaaatta ttagttgaag aaactgaaga aagacaacaa tcattcttcg
    39421 aaatgctagg agaatatatt ttagatggat ttaaagtagc tgtcattgta ggcgcaatgt
    39481 taatcggata cattgccatt atcgctctat taaatggaat tgtaagcaat atctttagta
    39541 cagtttcagg tggcgctatt tcttgggatt tccaaacatt aattggtttt gtctttgcac
    39601 catttgcatt cttagtcggt gtgccatggc aagatgcagt tcaagccggt tcagttatgg
    39661 cgacaaaact attatctaac gaatttgtag caatgcaagc acttggtaaa cttagtgatt
    39721 tatctgaaca cgcaaaaggt gttacatctg tattcttagt atcatttgct aactttagct
    39781 ctatcggtat tatttcaggt gcgattaaat cacttaatga caaaaaagga gatacagttg
    39841 cccgctttgg tttgaaatta ttattcggtg caacactagt atctttcatt tcagctgcaa
    39901 tcgctggatt ctttatttaa caaatacatc aacatcgaat taaccccttc aaaattttga
    39961 ctattctcag tctctttgaa gggttttttt cttatgtata gctttatttt ttatcatttt
    40021 taattttgac ataaaaaata actcctccaa agctaagttt gaaggagata ttttcttctc
    40081 aacaaatcta taaatatcaa taattcgttg atttagcata cattaagcta tgcttttttt
    40141 acaatatgat taataaagta atcagttact ctatagtcat ctgttaattc aggatggaat
    40201 gatacgccta aatatttacc ttgctgaaca gctacaattt tctcattaac cttacatagg
    40261 atatctacgc cttgacctac tttttcaata tgtggggctc ttataaagac accttcaata
    40321 tctgtagcga tacctttaat atctaattct gtttcaaagc tgtcaacttg tctaccgaat
    40381 gagtttcgtt gtacagtaat attcaacttg ttaaggtatc cttcttcacc aactatatct
    40441 tgcgctagaa ctattaatcc tgcgcatgta ccaaacatag gtaaagttga attttgtaaa
    40501 gcctctttaa atccatataa attcattaat cgacgtaacg ttgtagactc gccaccaggt
    40561 aatattaagc cctcgatttc ttctaattgt tcaacttttt taactgcaat accttcatga
    40621 ccacttaatt caatatgtct aatatgttca cgtactgcac cttgtaatgc taatacacct
    40681 attttcatat cttaccaacc acgctcttgc atacgttctt ctaatgataa ttgattgata
    40741 tctaaacctt tcatagcagt gccaagttca cttgctaatc ttccaattag ttcatagtct
    40801 tggtaatgtg ttgttgcttg aacaattgct ttagcaaatt tttctggatc ttctgattta
    40861 aaaatacctg atccaacgaa tacaccgtca gcacctaatt ccatcattaa agcagcatct
    40921 tgaggagtcg caacgccacc agctgcaaag ttaactaccg gtaaacgacc attgtcttta
    40981 atttgtttta aaatttcata aggcgcaccg atatctttcg caaaagtcat aatctcatca
    41041 tcattcatta cagtcaatcg actaacttct gaattaactt gtctcatatg tcttacagct
    41101 tcaacaatat tacctgtacc tggttcacct ttagtacgta acatagcagc accttcacca
    41161 attctacgcg cagcttcacc taaattacga catccacata caaatggtac tgtaaattga
    41221 tcttttctta agtgatattc ctcatctgct ggtgttaaca cttctgattc atcaatatag
    41281 tcaacaccca tcgcctctaa tactcttgct tcagtgatat gaccaatacg tgctttagcc
    41341 atgactggaa tagaaacagc attcattact tcttctacaa ttttagggtt tgccatacgt
    41401 gcaacaccac cagcagctct aatatcagaa ggtactcgtt ctaatgccat aactgctacc
    41461 gcgccagctt cttctgcaat tcttgcttgc tcagcattaa cgacatccat aataacgccg
    41521 cctttttgca tttcagccat acctcttttg actctgtctg atccaataat tttactcatt
    41581 atacataaaa ccccttttca agttgattaa atacattgta atgtataaac tgatatttaa
    41641 aaaggtacaa aataagataa atttaggagg tcagttataa aattgaaaaa cactttatac
    41701 catcaacttt atgaaaaatt aaaaaaacaa ataattgaag gtcaatttaa agaaggcgat
    41761 aaattttatt ctaaaagaca attaagcaaa catttatcta ttagccaaac aaccgtagag
    41821 catgcatatc aacttctatt agacgaaggt tacatttatt caagacctcg ctctggatat
    41881 tttgtgtccg aaattgaatc tttaacaatt ttaaacaatc aacctatccc ctctcttttt
    41941 gatgatgatt catataaacc aaaagcgagt gatgaagctt atgattatgc ttttaatttg
    42001 gatgaaatag atacgaaaca tttccctata gaacttttca gaaagtattc caaagatctt
    42061 tatgatacaa atcatttaaa tcaattacgt agaggacatt ttcaaggaga attacatttg
    42121 cggtttcaac tggcatttta tttatttact aatcgtggcg taatttgtga tcctaatcaa
    42181 attataattg gctcatctac cgaacaatta gtaaatcaat tagtcgattt actttatact
    42241 tcaacattta ttatcgaaaa acctagttat ccaccaatca aaaatatttt agataaaaaa
    42301 caagttgaat atgaacaaat tgaagttgaa gataatggaa taaacgtaga tgaagttatt
    42361 aaatcacaaa aaaatatagt atatataacg ccttcccatc aatttccaac tggatatgtg
    42421 atggacttaa aaaaacgaac tcaattaata caatgggctc aagaaaaaga agaacgattt
    42481 attatagaag atgattatga ttccgagttt agatatttcg gcaaacccat tccagcaatt
    42541 caaggtctat attcaagagg agaaaaagtg atttatatca gtacattttc aaagtctatt
    42601 tttcctagct gtcgagttgc atatatggtg ttaccctatt ctataatgaa aaaatatcat
    42661 tctcaaaatc acattgaagg caacactgta cctgttcata tgcaaaacct tattgcaacg
    42721 tttatatctt ccggtggttt tgaaaggcat ttaaataaaa tgagacgaat ttatcgtaga
    42781 aaactaacct atattttaaa aagattgaaa ccttacaaag agcagcttga tattcaaggt
    42841 gctgaaacgg gtatgcattt tacaattaca gtaaaaaatg gattaacatt acaagagtgt
    42901 cttgatagag caaacaaagt aaagttaaaa ttacaagttt ataattttga tgatgatcaa
    42961 gactataaga aaacccctaa atttatatta ggctttggcg gtatagacga tgatgcttta
    43021 aaaccgcatg tagacgctct aataaaatca ttaattataa agtaacactt ctttgataaa
    43081 agttagtgtt acttatgcaa tagcttgtct attgtataat aattaatttc ctttttgtat
    43141 ttcaataaag tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct
    43201 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
    43261 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
    43321 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
    43381 tgacaatcgc ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa
    43441 actcgttgcg ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
    43501 tttctttgtg tttgcttttt attttgacgt tttagacat
//
LOCUS       DALAAR010000015        42701 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000015 DALAAR010000000
VERSION     DALAAR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42701
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.015"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            115..4425
                     /locus_tag="O2458_000816"
     CDS             115..4425
                     /locus_tag="O2458_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ3886700.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            4715..5182
                     /gene="rimP"
                     /locus_tag="O2458_000817"
     CDS             4715..5182
                     /gene="rimP"
                     /locus_tag="O2458_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCZ3886701.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            5203..6378
                     /gene="nusA"
                     /locus_tag="O2458_000818"
     CDS             5203..6378
                     /gene="nusA"
                     /locus_tag="O2458_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCZ3886702.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            6399..6683
                     /locus_tag="O2458_000819"
     CDS             6399..6683
                     /locus_tag="O2458_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCZ3886703.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            6680..6997
                     /locus_tag="O2458_000820"
     CDS             6680..6997
                     /locus_tag="O2458_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCZ3886704.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            7002..9119
                     /gene="infB"
                     /locus_tag="O2458_000821"
     CDS             7002..9119
                     /gene="infB"
                     /locus_tag="O2458_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCZ3886705.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            9505..9855
                     /gene="rbfA"
                     /locus_tag="O2458_000822"
     CDS             9505..9855
                     /gene="rbfA"
                     /locus_tag="O2458_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCZ3886706.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            10024..10941
                     /gene="truB"
                     /locus_tag="O2458_000823"
     CDS             10024..10941
                     /gene="truB"
                     /locus_tag="O2458_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCZ3886707.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            10956..11927
                     /gene="ribF"
                     /locus_tag="O2458_000824"
     CDS             10956..11927
                     /gene="ribF"
                     /locus_tag="O2458_000824"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCZ3886708.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            12042..12311
                     /gene="rpsO"
                     /locus_tag="O2458_000825"
     CDS             12042..12311
                     /gene="rpsO"
                     /locus_tag="O2458_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCZ3886709.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            12681..14777
                     /gene="pnp"
                     /locus_tag="O2458_000826"
     CDS             12681..14777
                     /gene="pnp"
                     /locus_tag="O2458_000826"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCZ3886710.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            15013..16686
                     /locus_tag="O2458_000827"
     CDS             15013..16686
                     /locus_tag="O2458_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ3886711.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            16942..19311
                     /locus_tag="O2458_000828"
     CDS             16942..19311
                     /locus_tag="O2458_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ3886712.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            19316..20029
                     /locus_tag="O2458_000829"
     CDS             19316..20029
                     /locus_tag="O2458_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3886713.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            20060..21325
                     /locus_tag="O2458_000830"
     CDS             20060..21325
                     /locus_tag="O2458_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ3886714.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            21325..22611
                     /locus_tag="O2458_000831"
     CDS             21325..22611
                     /locus_tag="O2458_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ3886715.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            22611..23315
                     /locus_tag="O2458_000832"
     CDS             22611..23315
                     /locus_tag="O2458_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ3886716.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            23420..24247
                     /locus_tag="O2458_000833"
     CDS             23420..24247
                     /locus_tag="O2458_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCZ3886717.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            24266..24658
                     /locus_tag="O2458_000834"
     CDS             24266..24658
                     /locus_tag="O2458_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3886718.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            24692..25270
                     /gene="pgsA"
                     /locus_tag="O2458_000835"
     CDS             24692..25270
                     /gene="pgsA"
                     /locus_tag="O2458_000835"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCZ3886719.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            25497..26648
                     /locus_tag="O2458_000836"
     CDS             25497..26648
                     /locus_tag="O2458_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCZ3886720.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            26813..27856
                     /gene="recA"
                     /locus_tag="O2458_000837"
     CDS             26813..27856
                     /gene="recA"
                     /locus_tag="O2458_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCZ3886721.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            28210..29769
                     /gene="rny"
                     /locus_tag="O2458_000838"
     CDS             28210..29769
                     /gene="rny"
                     /locus_tag="O2458_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCZ3886722.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(30066..30281)
                     /locus_tag="O2458_000839"
     CDS             complement(30066..30281)
                     /locus_tag="O2458_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886723.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            30455..31252
                     /locus_tag="O2458_000840"
     CDS             30455..31252
                     /locus_tag="O2458_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCZ3886724.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            31392..33152
                     /locus_tag="O2458_000841"
     CDS             31392..33152
                     /locus_tag="O2458_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCZ3886725.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            33153..34019
                     /locus_tag="O2458_000842"
     CDS             33153..34019
                     /locus_tag="O2458_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCZ3886726.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            34113..34406
                     /locus_tag="O2458_000843"
     CDS             34113..34406
                     /locus_tag="O2458_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCZ3886727.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            34540..36084
                     /gene="miaB"
                     /locus_tag="O2458_000844"
     CDS             34540..36084
                     /gene="miaB"
                     /locus_tag="O2458_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCZ3886728.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            36085..36450
                     /locus_tag="O2458_000845"
     CDS             36085..36450
                     /locus_tag="O2458_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCZ3886729.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            36477..36968
                     /gene="thiW"
                     /locus_tag="O2458_000846"
     CDS             36477..36968
                     /gene="thiW"
                     /locus_tag="O2458_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCZ3886730.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            37271..39889
                     /gene="mutS"
                     /locus_tag="O2458_000847"
     CDS             37271..39889
                     /gene="mutS"
                     /locus_tag="O2458_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCZ3886731.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            39902..41911
                     /gene="mutL"
                     /locus_tag="O2458_000848"
     CDS             39902..41911
                     /gene="mutL"
                     /locus_tag="O2458_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCZ3886732.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            41917..42459
                     /locus_tag="O2458_000849"
     CDS             41917..42459
                     /locus_tag="O2458_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCZ3886733.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT        15884 a         5757 c         8193 g        12867 t
ORIGIN      
        1 atgattagcc aaatataaag catttaattt tctatcatta ctatgctcac ataatctaaa
       61 tattgttcga acacgtaaaa gtaatttcta tttaaggtgg taattgtctt ggcaatgaca
      121 gagcaacaaa aatttaaagt gcttgctgat caaattaaaa tttcaaatca attagatgct
      181 gaaattttaa attcaggtga actgacacgt atagatgttt ctaacaaaaa cagaacatgg
      241 gaatttcata ttacattacc acaattctta gctcatgaag attatttatt atttataaat
      301 gcaatagagc aagagtttaa agatatcgcc aacgttacat gtcgttttac ggtaacaaat
      361 ggcacgaatc aagatgaaca tgcaattaaa tactttgggc actgtattga ccaaacagct
      421 ttatctccaa aagttaaagg tcaattgaaa cagaaaaagc ttattatgtc tggaaaagta
      481 ttaaaagtaa tggtatcaaa tgacattgaa cgtaatcatt ttgataaggc atgtaatgga
      541 agtcttatca aagcgtttag aaattgtggt tttgatatcg ataaaatcat attcgaaaca
      601 aatgataatg atcaagaaca aaacttagct tctttagaag cacatattca agaagaagac
      661 gaacaaagtg cacgattggc aacagagaaa cttgaaaaaa tgaaagctga aaaagcgaaa
      721 caacaagata acaacgaaag tgctgtcgat aagtgtcaaa ttggtaagcc gattcaaatt
      781 gaaaatatta aaccaattga atctattatt gaggaagagt ttaaagttgc aatagagggt
      841 gtcatttttg atataaactt aaaagaactt aaaagtggtc gccatatcgt agaaattaaa
      901 gtgactgact atacggactc tttagtttta aaaatgttta ctcgtaaaaa caaagatgat
      961 ttagaacatt ttaaagcgct aagtgttggt aaatgggtta gggctcaagg tcgtattgaa
     1021 gaagatacat ttattagaga tttagttatg atgatgtctg atattgaaga gattaaaaaa
     1081 gcgacaaaaa aagataaggc tgaagaaaag cgtgtagaat tccacttgca tactgcaatg
     1141 agccaaatgg atggtatacc caatattggt gcgtatgtta aacaggcagc agactgggga
     1201 catccagcca ttgcggttac agaccataat gttgtgcaag catttccaga tgctcacgca
     1261 gcagcggaaa aacatggcat taaaatgata tacggtatgg aaggtatgtt agttgatgat
     1321 ggtgttccga ttgcatacaa accacaagat gtcgtattaa aagatgctac ttatgttgtg
     1381 ttcgacgttg agacaactgg tttatcaaat cagtatgata aaatcatcga gcttgcagct
     1441 gtgaaagttc ataacggtga aatcatcgat aagtttgaaa ggtttagtaa tccgcatgaa
     1501 cgattatcgg aaacgattat caatttgacg catattactg atgatatgtt agtagatgcc
     1561 cctgagattg aagaagtact tacagagttt aaagaatggg ttggcgatgc gatattcgta
     1621 gcgcataatg cttcgtttga tatgggcttc atcgatacgg gatatgaacg tcttgggttt
     1681 ggaccatcaa cgaatggtgt tatcgatact ttagaattat ctcgtacgat taatactgaa
     1741 tatggtaaac atggtttgaa tttcttggct aaaaaatatg gcgtagaatt aacgcaacat
     1801 caccgtgcca tttatgatac agaagcaaca gcttacattt tcataaaaat ggttcaacaa
     1861 atgaaagaat taggcgtatt aaatcataac gaaatcaaca aaaaactcag taatgaagat
     1921 gcatataaac gtgcaagacc tagtcatgtc acattaattg tacaaaacca acaaggtctt
     1981 aaaaatctat ttaaaattgt aagtgcatca ttggtgaagt atttctaccg tacacctcga
     2041 attccacgtt cattgttaga tgaatatcgt gagggattat tggtaggtac agcgtgtgat
     2101 gaaggtgaat tatttacggc agttatgcag aaggaccaga gtcaagttga aaaaattgcc
     2161 aaatattatg attttattga aattcaacca ccggcacttt atcaagattt aattgataga
     2221 gagcttatta gagatactga aacattacat gaaatttatc aacgtttaat acatgcaggt
     2281 gacacagcgg gtatacctgt tattgcgaca ggaaatgcac actatttgtt tgaacatgat
     2341 ggtatcgcac gtaaaatttt aatagcatca caacccggca atccacttaa tcgctcaact
     2401 ttaccggaag cacattttag aactacagat gaaatgttaa acgagtttca ttttttaggt
     2461 gaagaaaaag cgcatgaaat tgttgtgaaa aatacaaacg aattagcaga tcgaattgaa
     2521 cgtgttgttc ctattaaaga tgaattatac acaccgcgta tggaaggtgc taacgaagaa
     2581 attagagaac taagttatgc aaatgcgcgt aaactgtatg gtgaagacct gcctcaaatc
     2641 gtaattgatc gattagaaaa agaattaaaa agtattatcg gtaatggatt tgcggtaatt
     2701 tacttaattt cgcaacgttt agttaaaaaa tcattagatg atggatactt agttggttcc
     2761 cgtggttcag taggttctag ttttgtagcg acaatgactg agattactga agtaaacccg
     2821 ttaccgccac actatatttg tccgaactgt aaaacgagtg aatttttcaa tgatggttca
     2881 gtaggatcag gatttgattt acctgataag acgtgtgaaa cttgtggagc gccacttatt
     2941 aaagaaggac aagatattcc gtttgaaaca tttttaggat ttaagggaga taaagttcct
     3001 gatatcgact taaactttag tggtgaatat caaccgaatg cccataacta cacaaaagta
     3061 ttatttggtg aggataaagt attccgtgca ggtacaattg gtactgttgc tgaaaagact
     3121 gcttttggtt atgttaaagg ttatttgaat gatcaaggta tccacaaaag aggtgctgaa
     3181 atagatcgac tcgttaaagg atgtacaggt gttaaacgta caactggaca gcatccaggg
     3241 ggtattattg tagtacctga ttacatggat atttatgatt ttacgccgat acaatatcct
     3301 gccgatgatc aaaattcagc atggatgacg acacattttg atttccattc tattcatgat
     3361 aatgtattaa aacttgatat acttggacac gatgatccaa caatgattcg tatgcttcaa
     3421 gatttatcag gaattgatcc aaaaacaata cctgtagatg ataaagaagt tatgcagata
     3481 tttagtacac ctgaaagttt gggtgttact gaagatgaaa ttttatgtaa aacaggtaca
     3541 tttggggtac cagaattcgg tacaggattc gtgcgtcaaa tgttagaaga tacaaagcca
     3601 acaacatttt ctgaattagt tcaaatctca ggattatctc atggtacaga tgtgtggtta
     3661 ggcaatgctc aagaattaat taaaaccggt atatgtgatt tatcaagtgt aattggttgt
     3721 cgtgatgata tcatggttta tttaatgtat gctggtttag aaccatcaat ggcttttaaa
     3781 ataatggagt cagtacgtaa aggtaaaggt ttaactgaag aaatgattga aacgatgaaa
     3841 gaaaatgaag tgccagattg gtatttagat tcatgtctta aaattaagta catgttccct
     3901 aaagcccatg cagcagcata cgttttaatg gcagtacgta tcgcatattt caaagtacat
     3961 catccacttt attactatgc atcttacttt acaattcgtg cgtcagactt tgatttaatc
     4021 acgatgatta aagataaaac aagcattcga aatactgtaa aagacatgta ttctcgctat
     4081 atggatctag gtaaaaaaga aaaagacgta ttaacagtct tggaaattat gaatgaaatg
     4141 gcgcatcgag gttatcgaat gcaaccgatt agtttagaaa agagtcaggc gttcgaattt
     4201 atcattgaag gcgatacact tattccgccg ttcatatcag tgcctgggct tggcgaaaac
     4261 gttgcgaaac gaattgttga agctcgtgac gatggcccat ttttatcaaa agaagattta
     4321 aacaaaaaag ctggattatc tcagaaaatt attgagtatt tagatgagtt aggctcatta
     4381 ccgaatttac cagataaagc tcaactttcg atatttgata tgtaaaatga aataatcaag
     4441 gtatttattt aatgcgtatg gcgtagtcaa agaaatacaa aattgttgct ggacacaaaa
     4501 ttatgcccgt atttcttttc aatgtcttac gagtctattc aaatgtaatg gtgaaataaa
     4561 ggaacaaact tttacaagaa tctctgatta atagtgaagt catttgtttc aagcataaac
     4621 ttatgctata attaagttgc ttaaaaatta gtgaactcag gcagaagagt gggagattcc
     4681 cgctcttttc tatttgccaa aaagggaggc ctgtatgagt aaaattacag aacaagtaga
     4741 agtgattgtt aaaccaatta tggaagactt gaattttgaa cttgtagacg ttgaatatgt
     4801 caaagagggt agagatcatt ttcttagaat ctctattgat aaagaaggtg gcgtagattt
     4861 aaatgattgt acgctagctt ctgaaaaaat aagtgaagct atggatgcaa atgatcctat
     4921 tcctgaaatg tattatttag acgtagcgtc acctggtgca gaacgtccaa ttaaaaaaga
     4981 acaagatttc caaaatgcaa taactaaacc tgtatttgtt tctttatatg taccaattga
     5041 aggtgaaaag gaatggttag gcattttaca agaagtcaat aatgaaacaa ttgtagtaca
     5101 agttaaaatc aaagcaagaa cgaaagatat agagataccg agagacaaaa tagcaaaagc
     5161 acgtcacgca gttatgattt aacgtgatga ggaggaaaaa acgtgtcaag taatgaatta
     5221 ttattagcta ctgagtattt agaaaaagaa aagaagattc ctagagcagt attaattgat
     5281 gctattgaag cagctttaat tactgcatac aaaaagaatt atgatagtgc aagaaatgtc
     5341 cgtgtggaat taaatatgga tcaaggtact ttcaaagtta tcgctcgtaa agatgttgtt
     5401 gaagaagtat ttgacgacag agatgaagtg gatttaagta cagcgcttgt taaaaaccct
     5461 gcatatgaaa ttggtgatat atacgaagaa gatgtaacac ctaaagattt tggtcgtgta
     5521 ggtgctcaag cagcgaaaca agcagtaatg caacgtcttc gtgatgctga acgtgaaatt
     5581 ttatttgaag aatttataga caaagaagaa gacatactta ctggaattat tgaccgtgtt
     5641 gaccatcgtt atgtatatgt gaatttaggt cgtatcgaag ctgttttatc tgaagcagaa
     5701 agaagtccta acgaaaaata tattcctaac gaacgtatca aagtatatgt taacaaagtg
     5761 gaacaaacga caaaaggtcc tcaaatctat gtttctcgta gccatccagg tttattaaaa
     5821 cgtttatttg aacaagaagt tccagaaatt tacgatggta ctgtaattgt taaatcagta
     5881 gcacgtgaag ctggcgatcg ctctaaaatt agtgtcttct ctgaaaacaa tgatatagat
     5941 gctgttggtg catgtgttgg tgctaaaggc gcacgtgttg aagctgttgt tgaagagcta
     6001 ggtggtgaaa aaatcgacat cgttcaatgg aatgaagatc caaaagtatt tgtaaaaaat
     6061 gctttaagcc cttctcaagt tttagaagtt attgttgatg aaacaaatca atctacagta
     6121 gttgttgttc ctgattatca attgtcatta gcgattggta aaagaggaca aaacgcacgt
     6181 ctagctgcta aattaaccgg ctggaaaatt gatattaaat cagaaacaga tgcgcgtgaa
     6241 gcgggtatct atccagtagt tgaagctgaa aaagtaactg aagaagatgt tgctttagaa
     6301 gatgctgaca caacagaatc aaccgaagag gtaaatgatg tttcagttga aacaaatgta
     6361 gagaaagaat ctgaataata ggttggagtg aagtatctat gaaaaagaaa aaaattccga
     6421 tgcgaaaatg tattctttca aatgaaatgc atcccaaaaa agatatgatt cgtgttgttg
     6481 ttaataaaga aggcgaaatc tttgcggatg ttactggaaa gaaacaaggc cgtggcgcat
     6541 atgtttctaa agatgttgct atggttgaaa aagcacaaca aaaagaaatt ttagaaaaat
     6601 attttaaagc atctaaagag caattggatc ctgtttacaa agaaattatt agattaattt
     6661 atagagaaga gatcccaaaa tgagtataga tcaaatatta aactttttag gattagcaat
     6721 gagagctggt aaagtaaaaa caggtgaatc agtcattgtt aatgagatta aaaaaggaaa
     6781 tttgaagctc gttattgttg caaatgatgc gtctgataat acagctaaat taattacaga
     6841 taaatgtaag agttacaaag ttccattcag aaagtttgga aatcgaaatg aattgggaat
     6901 agcacttgga aaaggtgagc gtgttaatgt agggattact gacccaggct ttgctaaaaa
     6961 gttgctatca atgatagatg aatatcataa ggagtgatta tatgagtaaa caaagaattt
     7021 acgaatatgc gaaagaatta aatctaaaga gtaaagagat tatagatgag ttaaaaagca
     7081 tgaatattga ggtttcaaat catatgcaag ctttggaaga tgaccaaatt aaagcattag
     7141 ataaaaagtt caaaaaagaa caaaagaacg acaataaaca aagcactcaa aataatcacc
     7201 aaaaatcaaa caatcaaaac caaaataaag ggcaacaaaa agataacaaa aagaatcaac
     7261 aacaaaataa taaaggcaac aaaggcaata aaaagaataa tagaaataat aagaaaaata
     7321 acaagaataa taaaccacaa aatcaaccag ctgctccaaa agaaatacca tcaaaagtga
     7381 catatcaaga aggtattaca gtaggcgaat ttgcggataa attaaatgtt gaatcatcag
     7441 aaattatcaa aaaattattc ttacttggta ttgttgctaa tatcaatcaa tcattaaatc
     7501 aagaaacaat cgaattaatt gccgatgatt atggcgttga ggttgaagaa gaagttgtga
     7561 ttaatgaaga agacttatca atctatttcg aagacgaaaa agatgatcca gaggcaattg
     7621 agagaccagc agttgtaaca attatgggac atgttgacca tggtaaaacg actttattag
     7681 attcaattcg tcatacaaaa gttacagcag gtgaagcagg cggaatcact caacatattg
     7741 gtgcatatca aattgaaaac gatggcaaaa aaatcacttt cttagataca ccgggacatg
     7801 ctgcatttac aacgatgcgt gcgcgtggtg cacaagtaac agatattact attttagtag
     7861 tagcagctga cgatggtgtt atgccacaaa caattgaagc aattaaccat gctaaagaag
     7921 cagaagtacc aattattgtt gcagtaaata aaattgataa accaacttca aatcctgatc
     7981 gagttatgca agaattaact gaatatggtt taattcctga agattggggc ggcgaaacaa
     8041 ttttcgttcc actttctgca ttaagtggtg atggtatcga cgatttatta gaaatgatag
     8101 gattagttgc agaagttcaa gaacttaaag caaatcctaa aaaccgtgct gttggtacag
     8161 ttatcgaagc tgaattagat aaatcacgtg gtccttctgc atcattatta gtacaaaacg
     8221 gtacattaaa tgttggtgat gcgattgtag ttggtaatac ttacggccgt atccgtgcaa
     8281 tggttaatga cttaggtcaa agaatcaaaa cggctggtcc atcaacgcct gttgaaatta
     8341 caggtattaa tgatgtgcca caagctgggg atcgctttgt tgtatttagt gatgaaaaac
     8401 aagctcgtcg tattggtgaa tcaagacacg aagctagcat tatacaacaa cgtcaagaaa
     8461 gtaaaaatgt ttcattagat aacctgtttg aacaaatgaa acaaggtgaa atgaaagatt
     8521 taaacgttat tattaaaggt gatgttcaag gttctgttga agctttagct gcatcattaa
     8581 tgaaaattga tgttgaaggc gtaaatgttc gtatcattca tacagcggtt ggtgcaatta
     8641 atgagtcaga cgtgacactt gctaatgcct caaatggtat tatcattggt ttcaatgttc
     8701 gtccagacag tggtgcaaaa cgtgctgcag aagctgaaaa tgttgatatg cgtttacaca
     8761 gagttattta taatgttatc gaagaaattg aatcagcgat gaaaggttta cttgatccag
     8821 aatttgaaga acaagttatc ggacaagctg aagttcgtca aacattcaaa gtttctaaag
     8881 ttggtactat tgctggatgt tatgttactg aaggtaaaat tacgcgaaat gctggtgtac
     8941 gtattattcg tgatggtatt gttcaatatg aaggcgaatt agatacactt aaacgtttca
     9001 aagatgatgc taaggaagtt gcaaaaggtt atgaatgtgg tattacaatt gaaaactaca
     9061 atgaccttaa agaaggcgat gttatcgaag catttgaaat ggttgaaatt aagcgttaat
     9121 taaataaatt acaagctaaa agtatagtta agattgatat gctccctata aatattgcac
     9181 tttttaagtg tctactttat agggagcata tttgatacta gcttttggtt ttttattaga
     9241 atagattacc tattaaaagt tacgttatat ggacatgatt ttgtataaaa ttttgtggtg
     9301 gcctagaatg atttttaatg acaaaatata atgtcgacta ttattggaaa attttctgtt
     9361 gaaatgccta tcttacggca aactttattt gattttatag gcttaattta ttaaaataac
     9421 gtgtgagcta aaataattgt ttaagcattg ttacactaaa aaatgcaaat aacaattgaa
     9481 cttaaagata aagaggtgac aagaatgagc agtatgagag cagagcgtgt tggtgaacaa
     9541 atgaagaagg aattaatgga tatcatcaac aataaagtca aagatcctcg agttggtttt
     9601 attacaatta cagatgttgt tttaacaaat gatttatcgc aggctaaagt atttttaact
     9661 gtattaggta acgataaaga agtagaaaat acatttaaag cacttgataa agcaaaaggc
     9721 ttcattaagt ctgaattagg ttctagaatg cgattacgta ttatgccgga attaatgtat
     9781 gaatatgatc aatcaatcga atatggtaat aaaattgaac gaatgattca agatttacac
     9841 aaacaagata gataatttag tgttaggtat ctggaaaatg tttgataatt tcttaatatc
     9901 ggtatattaa cattaaacag ttaatacata gatgtgtaga aatagttaac attttccagt
     9961 ttttttatga ataaatttag ttgatacgct attaaaatat attttaaaaa agaaggtgac
    10021 tatatgtata atgggatatt accagtatat aaagagcgcg gtttaacaag tcatgacgtt
    10081 gtattcaaat tgcgtaaaat attaaaaact aaaaaaatag gtcacacggg tacgcttgat
    10141 cccgaagttg caggcgtgtt accggtatgt ataggtaatg caacgagagt tagtgattat
    10201 gttatggata tgggcaaagc ttatgaagca actgtatcga taggaagaag tacaacgact
    10261 gaagatcaaa cgggtgatac attggaaaca aaaggtgtac actcagcaga ttttaataag
    10321 gacgatattg accgattgtt agaaagtttt aaaggtatca ttgaacaaat tccgccgatg
    10381 tactcatccg tcaaagtaaa tggtaaaaaa ttatatgaat atgcgcgtaa taatgaaaca
    10441 gttgaaagac caaagcgtaa agttaatatt aaagacattg ggcgtatatc tgaattagat
    10501 tttaaagaaa atgagtgtca ttttaaaata cgcgtcatct gtggtaaagg tacatatatt
    10561 agaacgctag caactgatat tggtgtgaaa ttaggctttc cggcacatat gtcgaaatta
    10621 acacgaatcg agtctggtgg atttgtgttg aaagatagcc ttacattaga acaaataaaa
    10681 gaacttcatg agcaggattc attgcaaaat aaattgtttc ctttagaata tggattaaag
    10741 ggtttgccaa gcattaaaat taaagattcg cacataaaaa aacgtatttt aaatgggcag
    10801 aaatttaata aaaatgaatt tgataacaaa attaaagacc aaattgtatt tattgatgat
    10861 gattcagaaa aagtattagc aatttatatg gtacacccta caaaagaatc agaaattaaa
    10921 cctaaaaaag tctttaatta aaggagatag aatttatgaa agtcatagaa gtgacacatc
    10981 ctatacaatc taaacagtat attacagagg atgttgcaat ggcattcgga tttttcgatg
    11041 gcatgcataa aggtcatgac aaagtctttg atatattaaa cgaaatagct gaggcacgca
    11101 gtttaaaaaa agcggtgatg acatttgatc cgcatccgtc tgtcgtgttg aatcctaaaa
    11161 gaaaacgaac aacgtattta acgccacttt cagataaaat cgaaaaaatt agccaacatg
    11221 atattgatta ttgtatagtg gttaattttt catctaggtt tgctaatgtg agcgtagaag
    11281 attttgttga aaattatata attaaaaata atgtaaaaga agtcattgct ggttttgatt
    11341 ttacttttgg taaatttgga aaaggtaata tgactgtact tcaagaatat gatgcgttta
    11401 atacgacaat tgtgagtaaa caagaaattg aaaatgaaaa aatttctaca acttctattc
    11461 gtcaagattt aatcaatggt gagttgcaaa aagcgaatga tgctttaggc tatatatatt
    11521 ctattaaagg cactgtagtg caaggtgaaa aaaggggaag aactattggc ttcccaacag
    11581 ctaacattca acctagtgat gattatttgt tacctcgtaa aggtgtttat gctgttagta
    11641 ttgaaatcgg cactgaaaat aaattatatc gaggggtagc taacataggt gtaaagccaa
    11701 catttcatga tcctaacaaa gcagaagttg tcatcgaagt gaatatcttt gactttgagg
    11761 ataatattta tggtgaacga gtgaccgtga attggcatca tttcttacgt cctgagatta
    11821 aatttgatgg tatcgaccca ttagttaaac aaatgaacga tgataaatcg cgtgctaaat
    11881 atttattagc agttgatttt ggtgatgaag tagcttataa tatctagagt tgcgtatagt
    11941 tatataaaca atctatacca cacctttttc ttagtaggtc gaatctccaa cgcctaactc
    12001 ggattaagga gtattcaaac attttaagga ggaaattgat tatggcaatt tcacaagaac
    12061 gtaaaaacga aatcattaaa gaataccgtg tacacgaaac tgatactggt tcaccagaag
    12121 tacaaatcgc tgtacttact gcagaaatca acgcagtaaa cgaacactta cgtacacaca
    12181 aaaaagacca ccattcacgt cgtggattat taaaaatggt aggtcgtcgt agacatttat
    12241 taaactactt acgtagtaaa gatattcaac gttaccgtga attaattaaa tcacttggta
    12301 tccgtcgtta atcttaatat aacgtctttg aggttggggc atatttatgt tccaacctta
    12361 atttatatta aaaaagcttt ttacaaatat taacatttat tatatgttaa gctaatattg
    12421 agtgaataat aaggttacaa tgagataaag atgatataag tacacctaga gtaataatca
    12481 agatattaaa aataaagtat gtttttttaa aaaatataac ttatatttat actgataagg
    12541 gtgggacgat aagtctattt tgtaaataat agatggatat cccgctctct ttttttccaa
    12601 ttcaatattt tataactaat attaaaatac gataataaat gatatgatat aactattaga
    12661 ttcaagagag gagatttata atgtctcaag aaaagaaagt ttttaaaact gaatgggcag
    12721 gaagatcttt aacgattgaa acagggcaat tagctaaaca agcaaatggc gctgtattgg
    12781 ttcgttatgg agatacagtc gtgttatcga cggcaactgc atcaaaagaa cctcgtgatg
    12841 gagatttctt cccattaaca gtgaactatg aagaaaaaat gtacgctgcg ggtaaaattc
    12901 ctggtggatt taaaaagaga gaaggacgtc ctggtgacga tgcaacatta actgcgcgat
    12961 taattgatag accaattaga cctttattcc ctaaaggata taagcatgat gttcaaatta
    13021 tgaacatggt attaagtgca gatcctgatt gttcaccaca aatggctgca atgattggtt
    13081 catctatggc gcttagtgtg tcggatattc cattccaagg gccaatcgcc ggtgtaaatg
    13141 tgggttatat tgacggtaaa tatatcatta acccaacagt agaagaaaaa gaagtttctc
    13201 gtttagacct tgaagtagct ggtcataaag atgcggtaaa catggtagag gcaggcgcta
    13261 gtgagattac tgaacaagaa atgttagagg cgattttctt tggtcatgaa gagattcaac
    13321 gtttagttga tttccaacaa caaatcgtcg accacattca acctgttaaa caagaattta
    13381 ttccagcaga gcgtgatgaa gcgctagttg aacgtgtaaa atctttaacc gaagaaaaag
    13441 gacttaaaga aacagtttta acatttgata aacaacaacg agatgaaaat cttgataact
    13501 taaaagaaga aatcgtcaat gaatttatcg atgaagaaga tccagagaat gaattactta
    13561 ttaaagaagt ttatgcaatt ttaaatgaat tagtgaaaga agaagttcga cgtttaattg
    13621 cagatgaaaa aattagacca gacggccgta aacctgatga aatccgtcca ttagattctg
    13681 aagttggtat tttacctaga acgcatggtt caggtctatt tacacgtggt cagactcaag
    13741 cactttcagt tttaacatta ggtgctttag gcgattatca attaattgat ggtttaggac
    13801 ctgaagaaga aaaaagattc atgcatcatt acaacttccc gaatttttca gtaggtgaaa
    13861 ctggtccagt acgtgcgcca ggtcgtcgtg aaattggaca tggtgcgtta ggtgaaagag
    13921 cattaaaata tattattcct gatactgctg atttcccata tacaattcgt attgtaagtg
    13981 aggtacttga atcaaatggt tcatcatctc aagcgtcaat ttgtggatca acattagcat
    14041 taatggatgc gggcgtaccg attaaagcac cagttgctgg tattgctatg ggccttgtta
    14101 cacgtgaaga tagctatacg attttaactg atatccaagg tatggaagat gcattaggtg
    14161 atatggactt taaagtcgct ggtactaaag aaggtattac agcaatccaa atggatatta
    14221 aaattgacgg tttaacgcgt gaaattatcg aagaggctct agaacaagcg agacgtggtc
    14281 gtttagaaat aatgaatcat atgttacaaa caattgatca accacgtact gaattaagtg
    14341 cttacgcgcc aaaagttgta actatgacaa ttaaaccaga taagattaga gatgttatcg
    14401 gacctggtgg taaaaaaatt aacgaaatta ttgatgaaac aggtgttaaa ttagatattg
    14461 aacaagatgg tactatcttt attggtgctg ttgatcaagc tatgataaat cgtgctcgtg
    14521 aaatcattga ggaaattaca cgtgaagcgg aagtaggtca aacttatcaa gccactgtta
    14581 aacgtattga aaaatacggt gcgtttgtag gcctattccc aggtaaagat gcgttgcttc
    14641 acatttcaca aatttcaaaa aatagaattg aaaaagtgga agatgtatta aaaatcggtg
    14701 acacaattga agttaagatt actgaaattg ataaacaagg tcgagtaaat gcttcacata
    14761 gagcattaga agaataatat ttaaagtcat atgacgacaa tgtatcgtca tgtgattttt
    14821 ttatgccact ttttacgaag tgacccgttt tgaatttgtt gtattgaaca ttttaaaacg
    14881 ctttattatt ttgtgtgcaa ctgttaatta tcctgtatgt atagtgatta atagtgtaca
    14941 tcaagtgttt tttaacttat aatgaatagt gagtttatat atggacgggt aacaaattta
    15001 ggaggtaaga ttttgagttt aataaagaaa aagaataaag atattcgcat tataccatta
    15061 ggcggtgttg gcgaaattgc taaaaatatg tatatcgttg aagtagacga tgaaatgttt
    15121 atgttagatg ctggacttat gtttccagaa gacgaaatgc taggtattga tattgttata
    15181 ccagacattt catacgtact tgaaaataaa gataaattga agggtatatt ccttacacac
    15241 ggacatgagc acgcgattgg tgcagtgagt tatgttttag aacaattaga tgcaccagta
    15301 tatggatcta aattgacaat agcgttaatt aaagaaaata tgaaagcccg taatattgat
    15361 aaaaaagttc gctactatac agttaataat gattcaatta tgagattcaa aaacgtgaat
    15421 attagtttct ttaatacgac acacagtatt cctgatagtt taggtgtttg tattcacact
    15481 tcatatggtg ccattgtgta tacaggtgaa tttaagtttg accaaagttt acatggacat
    15541 tatgcaccag atattaaacg tatggcagag attggtgaag aaggcgtatt tgtcttaatc
    15601 agtgattcta ctgaggcaga gaaacctgga tataatactc cggaaaatgt gattgaacat
    15661 catatgtatg atgcttttgc aaaagtgcga ggtcgcttga tagtttcatg ttatgcttcg
    15721 aactttatac gtattcagca agttttaaat attgctagca agctaaatcg taaagtgtca
    15781 tttttaggaa gatcacttga aagttcattt aatattgctc gtaaaatggg gtatttcgac
    15841 attcctaaag atttgctaat tcctataaca gaagttgata attatcctaa aaatgaagtg
    15901 ataattatag ctactggtat gcaaggagaa cctgtagaag ccttaagtca aatggcgcaa
    15961 cataagcata aaattatgaa tatcgaagaa ggcgattctg tatttttagc aattacggct
    16021 tctgctaata tggaagttat cattgcgaat acattaaatg agcttgtacg tgctggcgca
    16081 catattattc caaataacaa aaagattcat gcttcaagtc atggttgcat ggaagaatta
    16141 aaaatgatga ttaatattat gaaacctgaa tactttattc ctgtacaagg tgaatttaaa
    16201 atgcagatag cacatgcgaa gctagcagct gaagcaggtg ttgcaccaga aaagattttc
    16261 cttgtggaaa aaggagatgt cattaattac aacggtaaag atatgatatt aaatgaaaag
    16321 gtaaattcag gaaatatttt aatagatggc attggtattg gggatgtagg aaatatcgtg
    16381 ttgagagacc gtcatctttt agcagaagat ggtatcttta ttgctgttgt aacgttagat
    16441 cctaaaaata gacgtatagc tgcgggacct gaaattcaat ctcgtgggtt tgtatatgta
    16501 cgtgaaagtg aagacttatt acgtgaagca gaagagaaag tacgtgaaat agtagaggct
    16561 ggtttacaag aaaaacgcat agaatggtct gaaattaaac aaaatatgcg tgatcaaatt
    16621 agtaaactat tattcgaaag tacaaaacgt cgtcctatga ttattccagt aatttctgaa
    16681 atttaatcaa aaagtcatta acataaaaga ggtcagaaca agtcactgaa atataatggt
    16741 tgtcatggac aatttactta tattttatga tggtcaattg aaggggtaac gattaatctg
    16801 ttatcttaag taaattgata catagatgat attgttctaa cctctttcat cgtctgtttg
    16861 gactacatat tctaaacatc aaataggaaa ttatatataa taacgtcgtt ttaactaagg
    16921 caacataagg aggtgcgtca attggcacaa gcaaaaaaga aatcgacagc taagaaaaaa
    16981 acaacatcaa aaaaaagaac aaattcgagg aaaaagaaga atgataatcc gatacgttat
    17041 gtcatagcta ttttagtagt tgtattaatg gtgttgggtg ttttccaatt aggaataata
    17101 ggtcgtctaa ttgacagctt ctttaattat ttatttgggt acagtagata tttaacatat
    17161 attttagtac tcttagcaac tggttttatt acatactcta aacgtattcc taaaactaga
    17221 cgaacggctg gttcgattgt attgcaaatt gcattgctat ttgtatcaca gttagttttt
    17281 cattttaata gtggtatcaa agctgaaaga gaacctgtac tttcttatgt gtatcagtca
    17341 taccaacaca gtcatttccc aaattttggt ggcggtgtat taggctttta tttattagag
    17401 ttaagcgtac ctttaatttc attatttggt gtatgtatta ttactatttt attattatgc
    17461 tcaagtgtta ttttattaac aaaccatcaa catcgtgaag ttgcaaaagt tgcactggaa
    17521 aatataaaag cttggtttgg ttcatttaat gaaaaaatgt cggaaagaaa ccaagaaaaa
    17581 caattgaagc gtgaagaaaa agcaagactt aaagaagaac aaaaggcacg tcaaaatgaa
    17641 cagccacaaa taaaagatgt gagtgatttt acggaagtgc ctcaagaaag agatattcca
    17701 atttatgggc atactgaaaa tgaaagtaaa agccagagtc aaccaagtcg aaaaaaacga
    17761 gtgtttgatg cagagaatag ttcgaataac atcgtaaatc atcatcaagc agatcagcaa
    17821 gaacaattaa cagaacaaac tcataacagt gttgaaagtg aaaacactat tgaagaagct
    17881 ggtgaagtta cgaatgtatc gtatgttgtt ccaccgttaa ctttacttaa tcaacctgca
    17941 aaacaaaaag caacatctaa agctgaagtg caacgtaaag gacaagtact agagaataca
    18001 ttaaaagatt ttggggtaaa tgcaaaagtg acacaaatta aaattggtcc tgcagtaact
    18061 caatatgaaa ttcaaccagc tcaaggggtt aaagtgagta aaattgtaaa cttgcataat
    18121 gatattgcat tagctttagc agcaaaagat gttagaatcg aagcgccaat acctggtcgt
    18181 tctgcagtag gtattgaagt gccaaatgag aaaatttcat tagtttcact aaaagaagtt
    18241 ttagatgaaa aattcccgtc taataataaa ctagaagttg gattaggaag agatatatca
    18301 ggtgatccaa ttactgttcc actaaatgaa atgccacact tattggtggc aggatcgacg
    18361 ggtagtggta aatctgtttg tataaatggt attattacaa gtattttatt aaatgctaag
    18421 ccgcatgaag ttaaacttat gttaatcgat ccgaaaatgg ttgaactaaa tgtttataac
    18481 ggaattccac acttattaat tccggttgtt acaaatcctc ataaagctgc tcaagcttta
    18541 gaaaaaattg tagctgagat ggaaagacgt tatgatttat tccaacattc atcaactaga
    18601 aacattaaag gttataacga attaatccgt aagcaaaatc aagaattaga tgagaagcaa
    18661 ccagaattac cttatatcgt tgttattgta gatgagcttg cagatttaat gatggtagct
    18721 ggtaaagaag ttgaaaatgc gattcaacgt attacacaaa tggcacgtgc agcaggtata
    18781 catttaattg tagcgacaca aagaccttct gtggatgtaa ttacaggtat cattaaaaat
    18841 aatattccat ctagaatagc ttttgctgtg agttctcaaa cagattcaag aactattatt
    18901 ggtactggcg gcgcagaaaa gttacttggt aaaggtgaca tgttatacgt tggaaatggt
    18961 gactcatcac aaacacgtat tcaaggggcg tttttaagtg accaagaggt gcaagatgtt
    19021 gtaaattatg tagtagaaca acaacaggca aattatgtaa aagaaatgga accagatgca
    19081 ccagtggata aatcggaaat gaaaagtgaa gatgctttat atgatgaagc gtatttgttt
    19141 gttgttgaac aacaaaaggc aagtacatca ttgttacaac gccaatttag aattggttat
    19201 aatagagcat ctaggttgat ggatgattta gaacgcaatc aggtaatcgg tccacaaaaa
    19261 ggaagcaagc ctagacaagt tttaatagat cttaataatg acgaggtgta aaaaaatgtc
    19321 agaaatgaat gcggtatata acgttaaaca atacatttta aatttgatta agcaaaataa
    19381 attggaatat ggtgaccaac ttccaagtaa tttatcaatt gccagagaat taaatgtaaa
    19441 aaccgacgat gtttatgaag caattcaggc attgattact gaacaagtca ttaaagataa
    19501 ttttgaagag ggcacaagtg ttaagtcact gccccctttc ttttatccat tgaatgaact
    19561 tataagtatt gggcaaatga ttaaaaatgc aggatttgaa tgcggaactg aatacttaaa
    19621 ttttgatgag caaccagcaa ctatgttaga tgcaaatttg ttgagcgttg aagaaggata
    19681 tccagtaacc attatagaac gattacgaac tgccgatgga gaaccggtcg tctattgttt
    19741 agataaaatt gctaaaaaag aattaacatg tacagagtat caaatgagca atggatcgat
    19801 tctaagtgcg ataaaagaac aaagtaatca taatatttgt tatgctgata cagaaattga
    19861 agcggtaaat tatgaacctc gaatatccga agtactgaat gcttcgccac acgaaggttt
    19921 gattttatta aaaattacgc actataatga atcagatgaa cctattttgt attcattaaa
    19981 ttatatgaaa aatagcttag ttcaatttaa aatcactaga aaaatataga tgtaagaaga
    20041 tatagaggag gctatatatt tgagtagaca atctcaacca aatatacata tcaaagtttc
    20101 accaacaact aaatttaaaa caactactat agtttttaaa tttatggcac ctttagaata
    20161 tgacacaata acagctagat cattattaag caaattatta gttcgagcaa ctaagaaatg
    20221 gccaaccgat aagtcgttta ataatcactt agccgattta tatggtgcgt atgtgaatag
    20281 tacaatttca aaattcaaag atcagcatgt cattacattt tcattagaaa ttgttaatga
    20341 acgttattta agaaacggtg aatcattatt taatcaagga ttagatttat tacaagaaat
    20401 catttggaat ccattaattg aaaataaagc attcaatgat aattttgtta accaagagaa
    20461 aacattatta gccaaaaaaa tagaagcaat ggtagataat aaagcacaat attcgttttt
    20521 aaaattactc gaccatatgt ttgaaaatga agcatataaa tacttatcta caggacaact
    20581 agaacaaatc ccacatatta ctgctgaaac actatatcat acatatcaat caatgattaa
    20641 taatgatcaa tgttctgttt atgttgtcgg caatgtagaa cctgaaagtg ttgagaaaca
    20701 aatacgtgaa aaatttgcac ttaaaccatt cgataaacat caattccaac attctactca
    20761 tcatttacac gatgaagaag ttgattatat tgttgaatat gatgacgtgg atcaagctaa
    20821 attaaatatg ggataccgtt ttccaacaca atatggacaa agtggatatg ctgcctttgt
    20881 tgtatttaac atgatgtttg gaggagatcc ttcatctgtt ttatttaatg aagtgcgaga
    20941 aaagcaaagt ttagcgtact ctatacattc acaaattgat ggcaaaaatg gctatttatt
    21001 tgttttgagt ggggtttcaa gtgataagta cgaaactgca aaagacacta ttataagtga
    21061 atttgaaaaa ataaaagcag gagatttcac tgaagaaaaa ttagagttag ctaaaaaagt
    21121 aatcatttct catcgatatg aatctgaaga tcgtccgaaa agtattatag agattatgca
    21181 taaccaaata ttattagagc aaccacaaag caaagaaaca tttataaatg atatacagaa
    21241 ggtaagtcgc gaagatattg tttctgttgc tgaaaaagca tttttagata caatctatgt
    21301 gttgacaaaa ggaggggata aataatgaaa gagcgttatt atgaattaat agacgaaaga
    21361 gtattcgaac aagaattaga aaatggttta cgattattta ttatccccaa accaggtttt
    21421 caaaagacat ttgtcactta cactacacaa tttggttcat tagataatca attcaaaccc
    21481 cttggacaag accaatttgt tactgtacct gatggagttg ctcacttttt agaacataaa
    21541 ttatttgaaa aagaagaaga agacttattt actgcgtttg ctgaagataa cgcacaagca
    21601 aatgcgttta caagctttga tcgtacaagc tacttgttca gtgcaactga taatattgaa
    21661 aacaacatta aacgtttact tacaatggtt gaaacgcctt attttacaaa agaaactgtt
    21721 gataaagaaa aaggtattat tgcagaagaa ataaaaatgt atcaagagca acctggatat
    21781 aaattaatgt ttgatacatt gcgtgcaatg tatcaacaac atccaatacg tgttgatatt
    21841 gccggtagtg tagaaagtat atacgatatt acaaaagatg atttgtatct atgttatgaa
    21901 acgttttatc atccatcaaa tatggtttta tttgttgttg gcgatgtgga tcctgaagca
    21961 atatgtcgaa tagtaaaaca acacgaggat gctcgtaata aagttaacca acccaaaatc
    22021 gaacgaggac ttgttgatga accggaggat gttaaagaag catttgttac tgaatctatg
    22081 aaaattcaat caccaagact aatgcttggt tttaaaaata aaccattaca agaagcgcct
    22141 caaaaatatg tacaacgtga tttagaaatg tcattattct ttgagttaat ttttggggaa
    22201 gaaacagatt tttatcagaa tttattaaac gaaggactta tcgatgatac atttggttat
    22261 caatttgtac tagagccgac gtatagtttt tcaatcgtga caagtgctac tgaagaacca
    22321 gataaattaa aaaaattatt attagatgag ttgcgtgata aaaaaggcaa tttccaagat
    22381 gcagaagcat ttgaactttt gaaaaagcaa tttataggtg aattcatatc aagtttaaac
    22441 tcacctgaat atattgctaa tcaatatact aaattgtatt ttgaaggtgt tagcgtgttt
    22501 gacatgttag acattgttga aaatatcacg ttagatagta ttaacgaaac gtcatcgtta
    22561 tatttaaatt tagatcagca agtcgatagt cgtttggaga ttaaaaagta atgaaagcat
    22621 tagtattagg tggttctggt tcaattggtt ctgagatagt caaacaatta ttaactgatg
    22681 gatttgaagt ttatgtgcaa tattatcgta ctgatataaa tgaattaact agcaaattta
    22741 atgatgataa agttcgtttt atacaagcgg atttatctca aacaattgat attgacaaaa
    22801 catttggtga cattaaatca ttagactgtt taatatatgc aagtggtcag tctttatatg
    22861 gtgttttaca agatatgaaa gaccatgata ttgatgcatg ttatcagtta aatgtcttgc
    22921 aattaattcg attatgtaga tatttcgttg atgttttacg tcaaagtgcc aatggaagaa
    22981 ttattgtaat ttcatcaatt tggggtgaga caggagctag tatggaaact atttattcgg
    23041 cgatgaaaag tgcacaatta ggtttcgtta aggcgcttag tcaagagctt gcactaacat
    23101 cagtgacagt aaatgctatc gcacctggat ttgtagccgg taatatggca agtgagtggc
    23161 aagaagatga acttcaagca atgataactg aattaccaca acagcgattg gttttaccga
    23221 gtgaggttgc tcatacatgc gcctatttat atcacccaaa tgctagaagt gttactggaa
    23281 ctatacagaa agttaatggt gcttggtata tttaaaatat agaactattt ttatgagcaa
    23341 atgtttttat gattgatgtt ataatatgaa tattacaatg aatcataatt tgttaattaa
    23401 acaaggggtg aggttcacca tgacagttgc agagaaaaaa gaatggtacc tagaatacga
    23461 aattgaaatt aatagaccgg gtcttttagg tgatgtatct agtttattag gtatgttagg
    23521 tataagtatt gttacaatta atggtgttga tcaaggtaaa cgaggccttt taattaaaac
    23581 agacaatctt gaaaaagttg aacgttttga gcaaatagct cgtggtataa atgaaattga
    23641 aataacaaag cttaaaaaac cagaattaag agaccgtctt gcagtaagac atggaagata
    23701 tattgagcaa gatgcaaaag ataagaaaac ctttcgattt gagcgtgaag atttaggctt
    23761 gttagtagac tttttagctg aattgttcaa agaagaaggt cataagttga ttggcattag
    23821 aggtatgcca cgggttggta aaactgaatc aattgttgcg ggaagtgttt gtgcacataa
    23881 gagatggtta tttattagtt ctactttaat aaaacaaact gtacgtagct ctctaattaa
    23941 aggggaatat gatgccaatc atgtatacat tattgatggt gcagttactg ccagagaatc
    24001 taatccaaaa catcaagagc ttgttaacga agttatgacg ttaccatcaa tcaaagtcgt
    24061 tgaacatcca gatttatttg ttgaaacaag tacttgtaca atggaagatt ttgactatat
    24121 tatcgaattg agagaaaatg aaaatcaaga aatacattac gaagaaatga agaaacagac
    24181 agtccaaagt aagaataatt tagattttgg agatccgttt ggtggtggtt ttggtttctt
    24241 cgagtaagtg taaggaggct atgaattgaa aacggtcggt gaagcgctaa aaggtagacg
    24301 tgaaaggtta ggaatgactt taacagaatt agagcaacgt actggaatta aacgtgaaat
    24361 gctagtgcat attgaaaata atgaattcga tcaactaccg aataaaaatt acagcgaagg
    24421 atttattaga aaatatgcaa gcgtagtaaa tattgaacct aaccaattaa ttcaagctca
    24481 tcaagatgaa attccatcga accaagccga atgggacgaa gtaattacag ttttcaataa
    24541 taataaagac ttagattata agagtaaatc aaaagagcca atacaattat tagtaatcat
    24601 gggtattaca gttttaataa ctttattgtt atggatcatg ttagttttaa tattttaaca
    24661 gaaataaatt agtgagaaat gaggatgtat aatgaatatt ccgaaccaga ttacggtttt
    24721 tagagtagtg ttaataccag tttttatatt gtttgcgtta gttgattttg gatttggcaa
    24781 tgtgtcattt ctaggaggat atgaaataag aattgagtta ttaatcagtg gttttatttt
    24841 tatattggct tcccttagcg attttgttga tggttattta gctagaaaat ggaatttagt
    24901 tacaaatatg gggaaatttt tggatccatt agcggataaa ttattagttg caagtgcttt
    24961 aattgtactt gtgcaactag gactaacaaa ttctgtagta gcaatcatta ttattgccag
    25021 agaatttgcc gtaactggtt tacgtttact acaaattgaa caaggattcg taagtgcagc
    25081 tggtcaatta ggtaaaatta aaacagcagt tactatggta gcaattactt ggttgttatt
    25141 aggtgatcca ttggcaacat tgattggttt gtcattagga caaattttat tatacattgg
    25201 cgttattttt actatcttat ctggtattga atacttttat aaaggtagag atgtttttaa
    25261 acaaaaataa atatttgttt atactagatt tcattttcat atggaatcta gtttttttaa
    25321 tcccaatttt agaaattagc cacgcaattg tttataatga tatattgtaa aacaatattt
    25381 gttcattttt ttagggaaaa tctgtagtag catctgatac attgaatcta aaattgatgt
    25441 gaatttttaa atgaaataca tgaaaaaatg aattaaacga tacaaggggg atataaatgt
    25501 caattgccat tattgctgta ggctcagaac tattgctagg tcaaatcgct aataccaacg
    25561 gacaatttct atctaaagta tttaatgaaa ttggacaaaa tgtattagaa cataaagtta
    25621 ttggagataa taaaaaacgt ttagaatcaa gtgtacgtca tgcgctagaa aaatatgata
    25681 ctgttatttt aacaggtggc ttaggtccta cgaaagatga cttaacgaag catacagtgg
    25741 cccagattgt tggtaaagat ttagttattg atgagccttc tttaaaatat attgaaagct
    25801 attttgagga acaaggacaa gaaatgacac ctaataataa acaacaggct ttagtaattg
    25861 aaggttcaac tgtattaaca aatcatcatg gcatggctcc aggaatgatg gtgaattttg
    25921 aaaacaaaca aattatttta ttaccaggtc caccgaaaga aatgcaacca atggtgaaaa
    25981 atgaattgtt gtcacatttt ataaaccata atcgaattat acattctgaa ctattaagat
    26041 ttgcgggaat aggtgaatct aaagtagaaa caatattaat agatcttatc gataaacaga
    26101 ctaatcctac gattgcgcct ttggcgggaa gtcatgaagt atatattaga ttgactgcaa
    26161 atgccgactc aaaagaacaa gcacaatcat tgattcaacc tgttaaacaa gaaattcttg
    26221 atcgtattgg agaatattat tatggttcag atgacacatt aattgagcaa gctgtaataa
    26281 agaaaattca tgaacctttt gtaatatatg atggtattac taatggtgct ttatatcatc
    26341 gattgaaaga agtggattta aacgatgttc taaagggtat gattaatcac aatgaaaact
    26401 ttgttgatat taataaacct attgagcagc aattaaaaga tgcagtgcaa tttgttaata
    26461 aattgtttaa tgtgtcatca gcaattattc tattagagta tgatggtgta gttcatatag
    26521 gctatgataa taactttgaa tttaaaactg agcaatttaa aatgtctaaa tctagaaatt
    26581 tattaaagaa cagaagtcaa aattatgcgc tcataagatt attaaattgg cttagaacaa
    26641 caaattaatt gtattatcga taaaaatata agcacgtttg ttcgtttttg cgttttgatt
    26701 tcaagatttt atacgaacaa atattcgcaa aacacttgta ttttattttg aatccttgta
    26761 tagtattggt aagataatta aaagatagca atttcaatta ggaggtctcg ctttggataa
    26821 cgatcgtcaa aaagctttag atacagtaat taaaaatatg gagaaatctt tcggtaaagg
    26881 tgccgtaatg aagttgggtg acaatatagg tcgccgagtt tcaactacat caactggttc
    26941 agttacatta gataatgcgc taggtgtagg tggctatcct aaaggacgaa ttattgaaat
    27001 ttatggtcct gaaagttctg gtaagacaac agtagcgctt cacgctattg ctgaagtaca
    27061 aagtaatggc ggggtggcag catttatcga tgctgaacat gctttagatc cagaatatgc
    27121 tcaagcatta ggcgtagata tcgataattt atatttatcg caaccggatc atggtgaaca
    27181 aggtcttgaa atcgccgaag catttgttag aagtggtgca gttgatattg tagttgtaga
    27241 ctcagttgct gctttaacac ctaaagctga aattgaagga gaaatgggag acactcacgt
    27301 tggtttacaa gctcgtttaa tgtcacaagc gttacgtaaa ctttcaggtg ctatttctaa
    27361 atcaaataca actgctattt tcatcaacca aattcgtgaa aaagttggtg ttatgttcgg
    27421 taatccagag actacaccag gtggacgtgc attaaaattc tatagttcag taagactaga
    27481 agtacgtcgt gcagaacagc ttaaacaagg acaagaaatt gtaggtaata gaactaaaat
    27541 taaagtcgtt aaaaataaag tggcaccacc atttagagta gctgaagttg atattatgta
    27601 tggacaaggt atttctaaag agggtgaact tattgattta ggtgttgaaa acgacatcgt
    27661 tgataaatca ggagcatggt attcttacaa tggcgaacga atgggtcaag gtaaggaaaa
    27721 tgttaaaatg tacttgaaag aaaatccaca aattaaagaa gaaattgatc gtaaattgag
    27781 agaaaaatta ggtatatctg atggtgatgt tgaagaaaca gaagatgcac caaagtcatt
    27841 atttgacgaa gaatagtaca caaatttata tctatagtta aacttagcaa atatccttat
    27901 aggattgatt gaaagtgata ttcatctcat aaagctagaa taatatctaa ctttatggga
    27961 tacactacaa atcgagacta taaggttttt tattttattt attattacat tatcaatagt
    28021 tttataatcg agcttcaaaa ctttagaaaa tagtagaaat agcattcaat atagtgcaaa
    28081 agtgcaaatt gataacttga cacttatctc ctataaaccg tacaattaat ttgtatgatt
    28141 tatatataat ttcataaagt catattgaat ttcatataaa gagcaaaccc tagaaaagga
    28201 ggtgtttgtg tgaatttatt aagcctccta ctcattttgc tggggatcat tctaggagtt
    28261 gttggagggt atgttgttgc ccgaaatttg ttgcttcaaa agcaatcaca agctagacaa
    28321 actgccgaag atattgtaaa tcaagcacat aaagaagctg acaatatcaa aaaagagaaa
    28381 ttacttgagg caaaagaaga aaaccaaatc ctaagagaac aaactgaagc agaactacga
    28441 gaaagacgta gcgaacttca aagacaagaa acccgacttc ttcaaaaaga agaaaactta
    28501 gagcgcaaat ctgatctatt agataaaaaa gatgagattt tagagcaaaa agaatcaaaa
    28561 attgaagaaa aacaacaaca agtagatgca aaagagagta gtgttcaaac gttaataatg
    28621 aagcatgaac aagaattaga acgcatctcc ggtctcactc aagaagaagc tattaatgag
    28681 caacttcaaa gagtagagga agaactgtca caagatattg cagtacttgt taaagaaaaa
    28741 gaaaaagaag ctaaagaaaa agttgataaa acagcaaaag aattattagc tacagcagta
    28801 caaagattag cagcagatca cacaagtgaa tcaacggtat cagtagttaa cttacctaat
    28861 gatgagatga aaggtcgaat cattggacga gaaggacgaa acatccgcac acttgaaact
    28921 ttaactggca ttgatttaat tattgatgac acaccagaag cggttatatt atctggtttt
    28981 gatccaataa gaagagaaat tgctagaaca gcacttgtta acttagtatc tgatggacgt
    29041 attcatccag gtagaattga agatatggtc gaaaaagcta gaaaagaagt agacgatatt
    29101 attagagaag caggtgaaca agctacattt gaagtgaacg cacataatat gcatcctgac
    29161 ttagtaaaaa ttgtagggcg tttaaactat cgtacgagtt acggtcaaaa tgtacttaaa
    29221 cattcaattg aagttgcgca tcttgctagt atgttagctg ctgagctagg cgaagatgag
    29281 acattagcga aacgagctgg acttttacat gatgttggta aagcaattga tcatgaagta
    29341 gaaggtagtc atgttgaaat cggtgtagaa ttagcgaaaa aatatggtga aaatgaaaca
    29401 gttattaatg caatccattc tcatcatggt gatgttgaac ctacatctat tatatctatc
    29461 cttgttgctg ctgcagatgc attgtctgcg gctcgtccag gtgcaagaaa agaaacatta
    29521 gagaattata ttcgtcgatt agaacgttta gaaacgttat cagaaagtta tgatggtgta
    29581 gaaaaagcat ttgcgattca ggcaggtaga gaaatccgag tgattgtatc tcctgaagaa
    29641 attgatgatt taaaatctta tcgattggct agagatatta aaaatcagat tgaagatgaa
    29701 ttacaatatc ctggtcatat caaggtgaca gttgttcgag agactagagc agtagaatat
    29761 gcgaaataat ttttgtctcc ctcacaaatt agtgagggag cttttttaag ttgtagtctt
    29821 aatctagtta gacagcactt tatcggtaat aactatatta aacagtagtt atttgaaagt
    29881 aagacggacc ttatattaaa taagaagtta ttgcttttaa taaaaatgtt ttaggcttcg
    29941 taattactat atttatatta tgtaaaccta taaagatgat tgcttttcta tccaataaaa
    30001 aagaagagaa gatgtaacac atcttctctt ctgcaatatt aattaggatt tatttctaag
    30061 ttgagttatt ttaattgtaa atctgttttc tttaattctt ttataacttc tgcagtatca
    30121 taacaatttg ttgcaattgt tgaatatctc tctgctaaac gatatgcatt aatgtaaagc
    30181 tttaaacttt ctttagctat atcctctgca tcttcgaatt ttgatgggtt agacataacc
    30241 actaattctg caaatttttc tggatcaata ttaatagaca tgtatttatt tacaactcct
    30301 atttattttg atgtcttaat actaacatat tgaagttttc agacaaagta atgtctctct
    30361 ataattgaag aaaaataatt caaagaattt aatatttact ttaaatatta gaacgtatga
    30421 attaaactag tatttatgag aggatgaaca aaacatgaga ataatgttta taggggatat
    30481 cgtaggtaaa attggacgag acgcaattga aacgtacata cctcaactga agcaaaagta
    30541 taaaccaaca gttacaattg taaatgctga aaatgcagca catggtaaag gtttgactga
    30601 aaaaatatat aaacaattac taagaaatgg tgtagatttc atgactatgg gtaatcacac
    30661 atatggtcaa cgtgaaattt atgattttat agatgaagca aaacgactag taagaccagc
    30721 gaattttccg gatgaagcgc cgggaattgg tatgagattt atacaaatta atgatattaa
    30781 acttgcagtt attaatctgc aaggaagagc gtttatgcca gatattgatg atccttttaa
    30841 aaaggcagat caattagtca aggaagcaca agaacaaact ccgtttatat ttgttgattt
    30901 tcatgcagaa acaacttctg aaaagtatgc aatgggatgg catttagatg gtagagctag
    30961 cgctgttgtt ggaacgcata cacacattca aacagcagat gaacgtattt taccaaaggg
    31021 gacagggtat ataacggatg ttggtatgac aggtttttat gatggcattt taggaataaa
    31081 taaaacagag gtaattgagc gttttatcac tagtttgcca caaagacatg ttgttccaaa
    31141 tgaaggtaga agtgtattat ctggtgttgt tattgattta gacaaagaag gtaaaacaaa
    31201 gcacatcgaa cgtatattga taaatgatga ccatccattt tcaacatttt aaaattacgt
    31261 aagtaaacat tcgaattgga ccctatcgtc cattagtatg aatttaatat agtaccactg
    31321 tttacatagt aaatcggtgg ttctttttgt tatcatttaa tatgaaatat atccatagga
    31381 ggcatataac tatgaaacca caattatcgt ggaaagttgg cggtcaacaa ggcgaaggta
    31441 ttgaatcaac tggggaaatc ttcgctacgg ctatgaatag aaaaggatat tatttatatg
    31501 gatatagaca tttttcaagt cgtatcaaag gtggacatac gaataataaa attagagttt
    31561 ctacgacgcc tgttcatgca attagtgatg atttagatat tttgattgca tttgaccaag
    31621 aaacaattga tgttaaccat catgaaatga gagaagacag tattatttta gctgatgcca
    31681 aggctaaacc tgtgaaacca gaaggatgtc atgcacagct tattgaatta ccttttacag
    31741 caaccgctaa agaattaggt acagcattaa tgaaaaacat ggttgcaata ggtgctacta
    31801 gcgcattgat gaatttgaat acaaatacat ttgaagaact tattactaat atgttttcta
    31861 aaaaaggtga caaggtagtt gaagtcaata tccaagcatt aaacgaaggt tatcaattaa
    31921 tgcaatctcg cttacctgaa atctacgggg actttgaatt agagtcaaca gatgcactac
    31981 cacatctata tatgattggt aacgatgcca ttggattagg tgcaattgct gcaggttcac
    32041 aatttatggc ggcatatcct attacacctg cgtctgaagt tatggaatat atgattgcca
    32101 atatatctaa agtaaacgga gcggttattc aaacagaaga tgaaattgct gctgtaacta
    32161 tggctattgg tgcaaattat ggtggagtca gagcgtttac ggctagtgct ggtccaggtt
    32221 tatctttaat gatggaagca attggattat ctggtatgac tgaaacgcca ttagtcatta
    32281 ttaataccca acgaggtgga ccttctactg gattacctac gaaacaagaa cagtcagatt
    32341 taatgcaaat gatttatggt acacatggtg atattccaaa aattgttgta gcaccaacag
    32401 atgcagaaga tgcattttat ttaactatgg aagcatttaa tttagcagaa caatatcaat
    32461 gccctgttat agttctaagt gatttgcaat tatctttagg taaacaaact gttgaaaaat
    32521 tagattataa tcgtattgaa attaaacgtg gtgaaatcat tcaatctgat attgaacgtg
    32581 aagaagatga taaaggttat ttcaagcgtt atgcgttaac atccgatggt gtttctccta
    32641 gacctatccc cggtgttaaa ggaggtattc atcatataac tggtgtggaa cacaatgaag
    32701 aaggtaaacc tagtgaatct gcgtcaaata gacaacaaca aatggaaaaa cgaatgcgta
    32761 aaattgagca gttactaatt gaatcgccag tagaagctaa cttacaacat gaggatgcag
    32821 atattcttta tatcggtttt atttctacaa aaggtgcaat tcaagaaggt agtaaccgtt
    32881 tgaatcaaca aggcataaaa gttaacacta tacaaattag acaattgcat ccattcccaa
    32941 caagcgttat tcaagatgca gttaataaag cgaagaaagt cgttgtagtg gagcacaatt
    33001 atcaaggaca attggctagt attataaaaa tgaatgtcaa tattcatgat aagattgaaa
    33061 attatacaaa gtatgatggg acacctttcc taccacatga aatcgaagaa aaaggcaaaa
    33121 taattgctac tgaaataaag gagatggtat agatggcgac atttaaagat tttagaaata
    33181 atgttaagcc taactggtgc cccggatgtg gcgatttctc agtacaagct gcaattcaaa
    33241 aagcagccgc aaatataggg ttagaacctg aagaagtagc tatcatcacc ggtataggat
    33301 gttctggccg tctttcagga tatattaatt cttatggcgt tcattctatt cacggacgtg
    33361 cattaccttt agctcaaggt gtaaaaatgg cgaataaaga tttaactgtt attgcatcgg
    33421 gaggagatgg tgatggttat gctataggta tggggcatac aatccatgct ttaagaagaa
    33481 atatgaacat gacgtatata gtcatggata atcaaattta tggtttgaca aagggacaaa
    33541 catcgccgtc atcagcagta ggatttgtta ctaaaacaac gccaaaaggt aatatagaaa
    33601 aaaatgttgc gcctttagaa ttagcattat catctggtgc cacatttgta gcccaaggtt
    33661 tttcaagcga tattaaagga ttaacaaaac taattgaaga tgcaattaat catgatggat
    33721 tttcattcgt taatgtcttt tcaccatgtg tgacttataa taaaattaac acatacgatt
    33781 ggtttaaaga acatttaaca agtgttgatg acattgaaaa ttatgattct acagataaac
    33841 aattagcgac taaaactgtt attgaacatg aatctttagt aactggtatt gtttatcaag
    33901 ataaagaaac accatcatat gaatctcaaa ttaaagagtt agatgataca ccacttgcta
    33961 aaagagatat caaaattact gaagacacct tcaatgcatt aactgaacaa tttatttaat
    34021 aattaaaact gagtcgtata aatgtattta taacagatcc atttatgcta ctcagttttt
    34081 tactattaca aaaaataaag gagtttttaa aaatgaaaga cacattaatg agtatacaaa
    34141 taattcctaa aacaccaaac aatgacaatg ttatacctta cgtagacgag gcgattaaaa
    34201 taattgacga atctggtttg cattttagag taggtccgtt agaaacgaca gtacaaggaa
    34261 atatgaatga atgtttaatt ttaatacaat cattaaatga acgaatggtg gaacttgaat
    34321 gtccaagtat tattagccaa gttaagtttt atcatgtgcc agatggcatc actattgaaa
    34381 ctttaactga aaaatatgat gaataacatt aaaagtgaag taaactggat ttgaattggc
    34441 ttgttagaga tgacgtataa ctttaactgt ttttgcactt tatagttaaa tttaatataa
    34501 ttattaaatg atacgggcaa atagaaagga ttttgtaaag tgaacgaaga acaaagaaaa
    34561 gcaagttctg tagatgtttt agctgagaga gataagaaag cagaaaaaga ttatagtaaa
    34621 tattttgaac atgtttatca gccgcctaat ttaaaagaag cgaaaaaaag aggtaaacaa
    34681 gaagttcgtt ataatagaga tttccaaatt gatgaaaaat atcgcggtat ggggaacgag
    34741 cgtacatttt taattaaaac atatggatgt caaatgaatg cacatgacac tgaggtcatt
    34801 gctggtatac ttgaagcatt aggctatcaa gcaacgactg atattaacac tgcagatgtt
    34861 attttaatta atacatgtgc gattagagaa aatgccgaga acaaagtgtt tagtgaaata
    34921 ggtaatttga agcatttgaa aaaagaacga cctgatattt taatcggtgt ttgtggttgt
    34981 atgtcacaag aagagtcagt agtgaataaa attttaaaat cgtatcaaaa tgtagatatg
    35041 atatttggta cacataatat tcatcattta ccagaaattt tagaagaagc atacttatct
    35101 aaagcaatgg ttgttgaagt atggtctaaa gaaggagacg ttattgaaaa tcttccaaaa
    35161 gtccgtgaag gcaacattaa agcatgggtc aatattatgt atggttgtga taagttttgt
    35221 acatattgta ttgttccatt tacaagaggt aaagaacgaa gccgtagacc tgaagacatt
    35281 atagatgaag tacgtgaact tgctcgtgaa ggttacaaag aaataacgct tttaggtcaa
    35341 aatgtaaatt cttatggtaa agatttacag gatatagaat atgacttagg agatctttta
    35401 caagcaattt ctaaaatagc gattccaaga gttcgtttca caacaagtca tccttgggac
    35461 tttacagatc acatgattga tgttatttca gagggtggta atatcgttcc gcatatccac
    35521 ttgccagttc aatctggaaa taatgcagta ttaaaaataa tgggtagaaa atatacacga
    35581 gaaagttatt tggatttagt aaaacgaatc aaagatagaa ttcctaatgt agcattaact
    35641 acagatatta ttgtagggta tccaaatgaa tcagaggaac aatttgaaga aactttaact
    35701 ctgtatgatg aagttggttt tgaacatgca tatacgtact tgtattcaca acgtgatggt
    35761 acgcctgctg ctaaaatgaa agataatgta cctttaaatg tcaaaaagga acgattgcaa
    35821 cgtttgaata aaaaagttgg tcattattca caaatagcta tgagtaagta cgaaggacaa
    35881 actgtaacag tactttgtga aggaagtagt aaaaaagatg atcaggttct tgctggctac
    35941 actgataaaa ataagctagt taatttcaaa gcgcctaaag aaatgattgg taaactagtg
    36001 gaagtacgaa tagatgaagc taaacagtat tcattaaatg gcagttttat aaaggaagta
    36061 gagccggaaa tggtgattca ataaatgtat aataaagatg acgtgttgaa ccaagcggat
    36121 aatattgcaa ataaaattaa aaatttggat actatcaaaa catatcaaca aattgaagca
    36181 cagattcatc agaaccaaac gataaagact aaaatggata tgttaaaaaa gcatcaaaaa
    36241 caagcagtaa actttcaaaa ttacgggaaa caaaatgcgc tagaacagtc ggaacatacc
    36301 attcagagta tagaagcaga aataaataca ttgcccatag ttgaacagtt tcaaacttca
    36361 caatatgaag cgaatcaatt attgaaaatg tttgtatcaa caatggaaac acgtttaaat
    36421 gaccataata aagccaagca tagtgattaa ttacaaacaa aaaggagaac aatcatatga
    36481 aatcaagaaa actggctata actgcacttt taattgcaat aaatgttgta ttaagcagta
    36541 ttatcatcat tcctctagga ccagttaagg cagcaccagt acagcatttt gtaaatgtat
    36601 taagtgcggt catagtaggt ccttggtatg gattagctca agcgcttata tcatcaattt
    36661 taagagttct ttttggtact ggtacagctt ttgcatttcc gggtagtatg attggagttt
    36721 tattggctag tatgttttac atatatcgta aacatatatt tatggccgcg gtcggtgaag
    36781 tacttggaac tggtgtcatc ggaagtttaa tttgtatacc attagcatat ttccttgggc
    36841 ttcaagactt cttcattaaa ccgttaatga ttacgttcat agtctcaagt gctatcggat
    36901 ctattataag ttatttctta ttaattactc taaaaaaacg tggtattctt caaaggttta
    36961 taaaataatt ttatgatata tgatttcaaa cagagtcatt gttcatttta atattgaacg
    37021 cccttactta attaaatgtc ttgaatgtta gaattttaat ttaattctaa acatgctatt
    37081 gacacatttt attaggtttg aggcgttttc ttatacattt aagatgcttc gtgattatat
    37141 ggtatgaata ggtttgtaaa agtaagtatg ttaaaatgta taagttaaat aattaataat
    37201 taaaaagctt acaaacgaat atattgtaaa gacaaatttt aaaaagagac aagggttgtg
    37261 aagaaataat atgtctaatg ttacaccaat gatgcagcaa tatttaaaaa taaaatcaga
    37321 ataccaagat tgcttattat tttttagact aggtgatttc tatgaaatgt tttatgaaga
    37381 tgccaaggag gcatcacgtg tacttgaaat tactttaact aaaagagatg ctaaaaaaga
    37441 aaatccaatt ccgatgtgtg gtgttccgta tcattctgca gatagttata tagatacact
    37501 tgttaataat ggatataaag tagctatttg tgaacagatg gaagatccga aacaaacgaa
    37561 aggtatggtt agacgtgagg tagtaagaat tgtgactcca ggaactgtga tggagcaagg
    37621 tggtgtagat gataaacaaa ataactatat tttaagtttt gttatgaatc aacctgaaat
    37681 tgcgcttagt tactgtgatg tttctactgg cgaattaaag gttacacatt ttaatgatga
    37741 agcgacttta ttaaatgaaa ttacgacgat aaaccctaac gaagttgtta tcaatgacaa
    37801 tatttccgat aatttaaaaa gacaaattaa tatggtgaca gaaacaataa cagtcaggga
    37861 aacgttatca tcagaaatct atagtgtgaa tcaaactgaa cataaattaa tgtatcaagc
    37921 gacacaatta ttgctagatt atattcatca tacacaaaaa cgtgatttat cgcatatcga
    37981 ggatgttgtt caatatgcag ctatagatta tatgaaaatg gatttttatg ctaagagaaa
    38041 ccttgagtta acggaaagca ttcgattaaa atcaaaaaaa ggaacgctac tttggctaat
    38101 ggacgaaacg aaaacaccaa tgggagcacg ccgcttaaaa caatggatag atagaccact
    38161 aataagtaaa gaacaaattg aagcacgatt agatatcgtt gatgaattta gtgctcattt
    38221 catagaaaga gacaccttaa gaacatatct taatcaagtg tatgatattg aacgtcttgt
    38281 tgggcgtgtt agttacggaa atgttaatgc gagagattta attcaactta aacattccat
    38341 ttctgaaata ccgaatatta aagcattact aaattctatg aatcagaata ctcttgtaca
    38401 agttaatcaa ctagaacccc ttgatgattt acttgatata ttagaacaga gtttagtaga
    38461 agaaccacca atttcagtta aagatggcgg actattcaaa gttggtttta atacgcaatt
    38521 agatgaatat cttgaagctt caaaaaacgg aaaaacatgg ttagcagaat tacaagccaa
    38581 agaaagacaa cgtacaggaa taaaatcatt gaaaataagc tttaataaag tgtttggtta
    38641 ttttatagaa ataacacgtg ccaacttgca aaattttgaa ccaagtgaat ttggttatat
    38701 gaggaagcaa acgttatcga atgctgaacg ttttataact gatgaactta aagaaaaaga
    38761 agatatcatt ttaggtgcgg aagacaaagc catcgaatta gaatatcaat tatttgttca
    38821 gctacgtgaa gaagttaaaa aatatactga acgtttacaa caacaagcta aaattatttc
    38881 agagctagat tgtttacaga gctttgcaga aattgctcaa aaatataatt acactaggcc
    38941 ttcatttagt gaaaataaaa cattagaatt agtggaatct aggcacccag tagtggaaag
    39001 agtaatggat tataatgact atgtgcctaa taattgtcga ttagataatg aaacatttat
    39061 atatttaatt acaggtccga atatgtctgg taaatcgaca tatatgagac aagttgccat
    39121 aattagtata atggcccaaa tgggagctta tgtcccttgt aaagaggcag tgttacctat
    39181 atttgatcaa atattcacta gaataggtgc ggcagatgat ttggtttcag gtaagagtac
    39241 gtttatggta gaaatgctag aagcacaaaa ggcattaact tatgcaacag aggatagttt
    39301 gattattttc gatgaaattg gacgtggtac ttcaacgtat gacggtttag ctttagcgca
    39361 ggcaatgata gagtatgtag ctgaaacatc acatgctaaa acgttatttt caacacatta
    39421 tcatgaattg acaacattag atcaagcatt accaagtcta aaaaatgttc acgtcgctgc
    39481 taatgaatat aaaggtgaac ttatattctt gcataaagtc aaagatggtg cagttgacga
    39541 tagttatggt attcaagttg cgaaattagc tgatttacct gaaaaagtta ttagcagagc
    39601 acaagtgatt ctaagcgagt ttgaagcgtc tgctggtaaa aaatcatcga tatcaaattt
    39661 aaaaatggtc gaaaatgaac ctgaaattaa tcaagaaaat ttaaacttaa gtgttgaaga
    39721 aacaactgat actttatctc aaaaagactt tgaacaagca tcatttgatt tgtttgaaaa
    39781 tgatcaagaa agcgagattg aactacaaat taaaaatttg aatttatcta atatgacacc
    39841 aattgaggca ttggtgaagt taagtgaatt acaaaatcaa ttaaaataga ggtgttgcaa
    39901 aatggggaaa attaaagaac tccaaacctc attagcaaat aaaatcgcag caggtgaagt
    39961 agttgaaaga ccgagttctg ttgtgaaaga actgttggaa aatgctatag atgcaggcgc
    40021 tacagaaata agcattgaag tagaggaatc tggcgtccaa tctattcgcg tagtcgataa
    40081 tggaagcgga attgaagcgg aagacttagg attagtattt catagacatg cgactagtaa
    40141 attagatcaa gatgaagatt tatttcatat taggacatta ggattccgtg gtgaagcact
    40201 agccagtatt tcatcagttg ctaaagtaac attgaagact tgcacggata atgctaatgg
    40261 aaatgaaata tatgtagaaa atggtgaaat attaaatcat aagcctgcaa aagcgaaaaa
    40321 aggaacagat atacttgtag aatcattatt ttataataca ccagcacgtt taaaatatat
    40381 taaaagttta tacactgaac taggtaaaat aacagatatt gtcaacagaa tggcaatgag
    40441 ccatccggac attcgaatag cactcatttc agatggcaaa acaatgttaa gtacaaatgg
    40501 ttcaggacga actaatgaag tgatggcaga gatttatggg atgaaagttg cacgagattt
    40561 agtacatata tctggagata caagtgatta tcacattgaa ggttttgttg caaagcctga
    40621 acattctaga agtaataagc actatatttc tatttttatt aatggacgat acattaaaaa
    40681 ctttatgcta aataaagcga ttttagaagg ctatcataca ctcttaacaa taggtaggtt
    40741 cccgatttgt tatattaata ttgaaatgga tccaatctta gtagacgtaa atgttcatcc
    40801 aacaaaacta gaagtgcgtt tatcaaaaga agagcaacta taccaattga ttgtgagcaa
    40861 aatacaagaa gcatttaaag accgtatatt aattcctaaa aataacttgg attatgtgcc
    40921 aaaaaaaaat aaagtattac attcattcga acaacaaaaa atcgaatttg aacaaagaca
    40981 aaacacagag aataaccaag agaagacgtt ttcatctgaa gaaagtaaca gtaagccatt
    41041 tatggaagaa aatcaaaacg atgagatagt tataaaagaa gattcatata atccattcgt
    41101 aacgaaaaca tctgaaagtt taatagctga tgatgaatct tctggttata ataatacacg
    41161 tgaaaaagat gaagactact tcaaaaaaca acaagaaatt ttacaagaaa tggatcaaac
    41221 atttgattca aatgatggta caactgtgca aaattatgag aataaagcgt ctgatgatta
    41281 ttatgatgta aacgatatta aaggaacaaa aagtaaagac cctaaacgaa gaattccata
    41341 tatggaaatt gttggacaag tacatggaac gtatattatt gctcaaaatg aatttggcat
    41401 gtacatgatt gaccagcatg cagctcaaga aagaataaaa tatgaatatt ttcgagataa
    41461 aataggtgag gttaccaatg aagtacaaga tttattaatc ccgttaacat ttcatttttc
    41521 aaaagatgaa caattagtca ttgatcaata taaaaatgag cttcaacaag taggtatcat
    41581 gttagaacat tttggtggtc atgattatat tgtaagtagc tatccagttt ggttccctaa
    41641 agatgaagta gaagaaatta ttaaagatat gattgagcta attttggaag agaaaaaagt
    41701 agatatcaaa aaattacgtg aagatgtagc aatcatgatg tcatgtaaaa aatccattaa
    41761 agcgaatcat tatttacaaa aacatgaaat gtctgattta attgatcaat taagagaagc
    41821 ggaagatcca tttacatgtc cacatggtcg tccaataatc attaattttt caaaatacga
    41881 attagaaaaa ttatttaagc gtgtgatgta gagaggatga atcaagtgaa taacaacata
    41941 ttgcctgcta taagaaacat taaagattta gagaaactga ttaaaacaga ctataaaatg
    42001 tgtgtgcttc tagatatgca tataggacat ataaaaagta ttatggaatt gctgaagcaa
    42061 aatcatatag agtgttttat tcatatagat ttgataaaag gtttaagcca cgatgaattt
    42121 gcaagtgaat ttattattca gcaatacaag ccaaaaggta tcgtatcgac taaatctaaa
    42181 gtaataaaaa aagctaaatc attaaatact ttaacgattt ttagagtatt tattattgat
    42241 agtcaagcat tgaaacgcag tatagatttg ataaaaaaag ttgaacctga ttttgttgaa
    42301 gtacttccag gtgttgcgag taaagcgatt catcatattc agaaagaaac aaacacacaa
    42361 gtcattgcag gtggcctaat taatacaata gatgaagtca atgaagctgt taaaaatgga
    42421 gcgaaatatg taactactag ttatgataaa ctttggtaaa tcataagtct gcaaaaattt
    42481 catttttcac gagatttagt catataacat aataaaaaag ccgttttcat atgggatatg
    42541 cagtaggtat caaacaaaaa ataatcaaat aaaataatta gcacctctaa aacgattaaa
    42601 atcattaaag ttttagaggt gtttattagt aaaaaatgca actagtcata aaaaatatgg
    42661 ctgaagaatg tcaattaaac aaaagagcgt cataaataaa t
//
LOCUS       DALAAR010000016        42316 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000016 DALAAR010000000
VERSION     DALAAR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.016"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(39..1166)
                     /locus_tag="O2458_000850"
     CDS             complement(39..1166)
                     /locus_tag="O2458_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ3886734.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1528..1905)
                     /locus_tag="O2458_000851"
     CDS             complement(1528..1905)
                     /locus_tag="O2458_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCZ3886735.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2321..2914)
                     /locus_tag="O2458_000852"
     CDS             complement(2321..2914)
                     /locus_tag="O2458_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCZ3886736.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2978..3361
                     /locus_tag="O2458_000853"
     CDS             2978..3361
                     /locus_tag="O2458_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCZ3886737.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3492..5897
                     /gene="mnhA1"
                     /locus_tag="O2458_000854"
     CDS             3492..5897
                     /gene="mnhA1"
                     /locus_tag="O2458_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCZ3886738.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5890..6318
                     /gene="mnhB1"
                     /locus_tag="O2458_000855"
     CDS             5890..6318
                     /gene="mnhB1"
                     /locus_tag="O2458_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCZ3886739.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6318..6659
                     /gene="mnhC1"
                     /locus_tag="O2458_000856"
     CDS             6318..6659
                     /gene="mnhC1"
                     /locus_tag="O2458_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCZ3886740.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6652..8148
                     /gene="mnhD1"
                     /locus_tag="O2458_000857"
     CDS             6652..8148
                     /gene="mnhD1"
                     /locus_tag="O2458_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCZ3886741.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8150..8629
                     /gene="mnhE1"
                     /locus_tag="O2458_000858"
     CDS             8150..8629
                     /gene="mnhE1"
                     /locus_tag="O2458_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCZ3886742.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8629..8922
                     /gene="mnhF1"
                     /locus_tag="O2458_000859"
     CDS             8629..8922
                     /gene="mnhF1"
                     /locus_tag="O2458_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCZ3886743.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8900..9256
                     /gene="mnhG1"
                     /locus_tag="O2458_000860"
     CDS             8900..9256
                     /gene="mnhG1"
                     /locus_tag="O2458_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCZ3886744.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9505..10659
                     /locus_tag="O2458_000861"
     CDS             9505..10659
                     /locus_tag="O2458_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ3886745.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10715..11089)
                     /locus_tag="O2458_000862"
     CDS             complement(10715..11089)
                     /locus_tag="O2458_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCZ3886746.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11108..12424)
                     /locus_tag="O2458_000863"
     CDS             complement(11108..12424)
                     /locus_tag="O2458_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCZ3886747.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12835..14310)
                     /locus_tag="O2458_000864"
     CDS             complement(12835..14310)
                     /locus_tag="O2458_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCZ3886748.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14441..15649)
                     /locus_tag="O2458_000865"
     CDS             complement(14441..15649)
                     /locus_tag="O2458_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3886749.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16103..16462)
                     /locus_tag="O2458_000866"
     CDS             complement(16103..16462)
                     /locus_tag="O2458_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCZ3886750.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16475..16711)
                     /locus_tag="O2458_000867"
     CDS             complement(16475..16711)
                     /locus_tag="O2458_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCZ3886751.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            17028..18092
                     /locus_tag="O2458_000868"
     CDS             17028..18092
                     /locus_tag="O2458_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3886752.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(18151..18474)
                     /locus_tag="O2458_000869"
     CDS             complement(18151..18474)
                     /locus_tag="O2458_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCZ3886753.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            18575..18817
                     /locus_tag="O2458_000870"
     CDS             18575..18817
                     /locus_tag="O2458_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCZ3886754.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(19080..20255)
                     /gene="dltD"
                     /locus_tag="O2458_000871"
     CDS             complement(19080..20255)
                     /gene="dltD"
                     /locus_tag="O2458_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCZ3886755.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(20252..20488)
                     /gene="dltC"
                     /locus_tag="O2458_000872"
     CDS             complement(20252..20488)
                     /gene="dltC"
                     /locus_tag="O2458_000872"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCZ3886756.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(20506..21720)
                     /gene="dltB"
                     /locus_tag="O2458_000873"
     CDS             complement(20506..21720)
                     /gene="dltB"
                     /locus_tag="O2458_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCZ3886757.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(21717..23174)
                     /gene="dltA"
                     /locus_tag="O2458_000874"
     CDS             complement(21717..23174)
                     /gene="dltA"
                     /locus_tag="O2458_000874"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCZ3886758.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(23190..23342)
                     /locus_tag="O2458_000875"
     CDS             complement(23190..23342)
                     /locus_tag="O2458_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCZ3886759.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(23390..23500)
                     /locus_tag="O2458_000876"
     CDS             complement(23390..23500)
                     /locus_tag="O2458_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886760.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(23754..24713)
                     /locus_tag="O2458_000877"
     CDS             complement(23754..24713)
                     /locus_tag="O2458_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCZ3886761.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(24746..25525)
                     /locus_tag="O2458_000878"
     CDS             complement(24746..25525)
                     /locus_tag="O2458_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCZ3886762.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(25525..25959)
                     /locus_tag="O2458_000879"
     CDS             complement(25525..25959)
                     /locus_tag="O2458_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCZ3886763.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            26057..26323
                     /locus_tag="O2458_000880"
     CDS             26057..26323
                     /locus_tag="O2458_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCZ3886764.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(26402..26785)
                     /locus_tag="O2458_000881"
     CDS             complement(26402..26785)
                     /locus_tag="O2458_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCZ3886765.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(26915..27832)
                     /gene="lipA"
                     /locus_tag="O2458_000882"
     CDS             complement(26915..27832)
                     /gene="lipA"
                     /locus_tag="O2458_000882"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCZ3886766.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(27916..29235)
                     /locus_tag="O2458_000883"
     CDS             complement(27916..29235)
                     /locus_tag="O2458_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ3886767.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(29262..30089)
                     /locus_tag="O2458_000884"
     CDS             complement(29262..30089)
                     /locus_tag="O2458_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ3886768.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(30102..30950)
                     /locus_tag="O2458_000885"
     CDS             complement(30102..30950)
                     /locus_tag="O2458_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCZ3886769.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(30998..31096)
                     /locus_tag="O2458_000886"
     CDS             complement(30998..31096)
                     /locus_tag="O2458_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886770.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            complement(31163..32230)
                     /locus_tag="O2458_000887"
     CDS             complement(31163..32230)
                     /locus_tag="O2458_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCZ3886771.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(32244..33284)
                     /locus_tag="O2458_000888"
     CDS             complement(32244..33284)
                     /locus_tag="O2458_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ3886772.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            33649..33963
                     /locus_tag="O2458_000889"
     CDS             33649..33963
                     /locus_tag="O2458_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886773.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(33969..34106)
                     /locus_tag="O2458_000890"
     CDS             complement(33969..34106)
                     /locus_tag="O2458_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCZ3886774.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(34293..35690)
                     /gene="sufB"
                     /locus_tag="O2458_000891"
     CDS             complement(34293..35690)
                     /gene="sufB"
                     /locus_tag="O2458_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCZ3886775.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(35841..36305)
                     /locus_tag="O2458_000892"
     CDS             complement(35841..36305)
                     /locus_tag="O2458_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCZ3886776.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(36295..37545)
                     /locus_tag="O2458_000893"
     CDS             complement(36295..37545)
                     /locus_tag="O2458_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ3886777.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(37651..38958)
                     /gene="sufD"
                     /locus_tag="O2458_000894"
     CDS             complement(37651..38958)
                     /gene="sufD"
                     /locus_tag="O2458_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCZ3886778.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(39056..39817)
                     /gene="sufC"
                     /locus_tag="O2458_000895"
     CDS             complement(39056..39817)
                     /gene="sufC"
                     /locus_tag="O2458_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCZ3886779.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            40145..40996
                     /locus_tag="O2458_000896"
     CDS             40145..40996
                     /locus_tag="O2458_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCZ3886780.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            complement(41229..41423)
                     /locus_tag="O2458_000897"
     CDS             complement(41229..41423)
                     /locus_tag="O2458_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ3886781.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
BASE COUNT        13313 a         7616 c         6263 g        15124 t
ORIGIN      
        1 ttgcatacac aatatcatta gtggtaacag ttaaacactt atttatcttt acgtggtaat
       61 ggataaaacc cttcattaaa ttgatgccat aaattaggag ttaagtgatg attatcttct
      121 ctctctggat caaagtaatt aataatttca tcttcccgtc catctttaat tttttcaaca
      181 aattgataat ccagtagtag ctctctacca atggctacta agtcaacacc aacattttca
      241 actgcatcta aagcttcgtc agctgtgaaa attgaaccaa taccgataag tggcatacga
      301 ccatttatcc atttgtgaat taaaggcagt ctttcttgtc cagcgtattt accttcacgt
      361 gtcgttgcat gcgtatccat cattgaaaca tgaatatagt cgattggcat atggctaatt
      421 ttattaacga gttcctctgt aatttccatt gtgattcctg gagactccgc ttcctctgga
      481 gataatctgt atccaactat aaagtcttta ttgccatacg cttctttcgc tttaagtact
      541 tcttcaatca cagcgaccgg gaatttatat tgatttgccc atacatcatt tcttctatta
      601 tagtatggtg atacaaattg atgaattaag taatgattcg cgccatgtat ttcaacacca
      661 tcaaaccctg cttcaattgc acgtcgcgtt gcttcaccaa aatccttgat tgcttgttca
      721 atctcttcat tcgtcatttc tctagcacta tgttcttgtt tctgaccaaa actttttaaa
      781 gaaattggac ttggtgctac gacgtctcca tcaggtgtta attcaggcaa tgcttgtgca
      841 ccgccatgat gtatttgtac gagtgctttg gcaccgtttt tcttcattgc tgtagctaat
      901 cgttttagtc cttcaatatt actgtcatgc gcgattgatg gctgtcctgg aaatgctttt
      961 ccgacatcac tcacattact cgcagcatta attgtaatac caacatcttg tgaacgcttt
     1021 tcaatataag gaagttctac atctgaaata gtaccatcat catttgaaga aatatgtgtt
     1081 aaaggggcta acacaaatcg atttctcaac tctactccat ttggtaattc tactttatca
     1141 aacaatggtt cgtatttact tttcattact taattcctcc ttaaaattat tgagttgact
     1201 ataacatgta attttttcat tctccaatta tctgtttata tagttgcaat tcgtttaata
     1261 ctttaagttg tagttctcat ttctaaaata aataaaaaaa acaaatccac atacaatatt
     1321 gcaaatcaac gattctatct aacaaagata atggatatgt caccttaaac catttgtacg
     1381 tgtaaataga atcaatcata ttacgttatc gtagacaaaa agagattagt atctaataaa
     1441 atttaataga tactaatctc ttttatagtc taagaaactt tcatttcaga ccctttgtat
     1501 gattttcggt acgatttatc tgttccttta gtcgtttcga attgctcgct ttgactgttt
     1561 tatccagatt ggtaagcgtt ctttaagtgt ttgaaaccca tatttttctg tttctctgat
     1621 ttcatctaat actgattgtt tttccttctt acgctcataa tttttgaagt aatcattatc
     1681 ctttaatgat agattaagct ttccttcatc atctatagac aaaattttag ctttaacaat
     1741 ttggccttct gatagaaatt tcttcaaatt atgaacgtag tcatccataa tttctgatat
     1801 atgaatcagt ccttcagtat gattaggggt ctcaacaaac gcaccgtatg gttgaatacc
     1861 agttacacgc accttgatat gttggccaat tttgtagtta ttcaacttaa taacccctta
     1921 tttcgtttac ttaacactat tatcatagca caaatcgaaa tggtggacta attaaaacat
     1981 caaatcatac gaatattatt ttataaatga ataagaattc tacaaacttg gatttacaaa
     2041 taaaattagc aacaaattcc tatctaaatc aatatgatat ccagctactt tgttttcaat
     2101 ttatttacta tttgaatagc aattctctgt aaaaacataa aaagcaacct caactattat
     2161 ctttaaaata cacatctatt taaaaactgt gctttcttga taaatatatt gagattgctc
     2221 atcatattag tttatttatt ttcgttggaa tatatactaa tccattcata aattagcttt
     2281 atccgagttt aaaatgttgg tagttaatta tgtttagata ttattcttca acatcaatag
     2341 attcaattac aacatcatga agtggtttat cttgtggtcc cacttttgta tttgcaatat
     2401 cttctaatgt agtttcacca tcaatgattt gaccgaatac tgtatgtttt tgatctaacc
     2461 atggtgtacc acccttttcg ccatatgcat caacgattgg ttgaggccag ccaccatctg
     2521 caagttgact taacatattt tgaggtactt ctttcatttg aacaatgaaa aattgtgaac
     2581 cattagtatt aggtcctgag ttagccattg ataatgcgcc atataagtta aatgcattta
     2641 atgaaaattc atcttcaaaa gcaccgccat aaatactttc gccacccata ccagtagctg
     2701 ttggatcgcc accttgaatc atgaagtcat taatgacacg gtggaatgtg attccatcat
     2761 aataaccatt ttttgcatgt gtcacaaaat tttcaactgt ttttggtgca atatttggaa
     2821 ataatttgaa tgtcatgtca cctttatttg tgtgcataac cactttgatt tcaccttgtt
     2881 gtacttcttt gtttaactgt ggatagttag ccatttatat tctccattca tgttatgata
     2941 catatatatt aacatacttt taagggagga aaatagcatg aagctatatc gattttcaca
     3001 caaaacaggt agctatggtg tcacgattgt tgaagaaaat ggagacgatg tccttgttaa
     3061 agttgaacaa gttatcaaac acccgaaaca aggtgattta cataacccta ctgaaactga
     3121 aggtgtattt ttccatgacc gcaaagcatt aagccatttt gaaaaacgct atacgaaacg
     3181 ttcacaatta cgcgacttca atgttgatgt attgcgctat gaagattcac ttcaacaagc
     3241 aattacagat ttagaaacta aacttaaagc tcagcaaaca aaacatgctg aaatgtcttt
     3301 agctagttta gcacgcttaa aagaagatta ttcacttcaa tacaaagaaa attttaaata
     3361 aaaaatgacg tgtttcttct tatttaaata cttctgaaaa taaagcacaa caaggagtgt
     3421 gtacgttttt cagaactata tcattcatgt ataatgaata acataaatat tttttaaagg
     3481 aggacaacat agtgagtttg ttacatattg cggtgatatt accgttaata ttcgcactca
     3541 ttattcctat cctgtataga ttttttaaac gtatacattt aggatggttc gtattacctg
     3601 tccctatcgt catatttatt tatatgctta cattaattaa aacgacgatg tcaggtaata
     3661 ctgtaatgaa aaccttaaat tggatgcctc attttggtat gaactttgat ttatatcttg
     3721 atggcttagg tttactcttt agtttgctta tttcaggaat cggcagttta gtagtattat
     3781 attcaatcgg ctacttaagt aagtcagaac aactagggaa tttttattgt tatttattac
     3841 tatttatggg tgcaatgtta ggtgtcgtac tttcagataa tgttattatt ctatatttat
     3901 tctgggagct aacgtcattt tctagttttt tacttatttc attctggcgc gaacgacaag
     3961 catctatata tggtgctcaa aaatcgctca taatcacagt atttggtggt ctatcattat
     4021 taggtggtat catcttactc gctataccaa cacaatcatt tagcattcag tacatgattc
     4081 aacatgcttc tgaaattcaa aatagcccat tcttcatttt tgcgatgatt ttaatcatga
     4141 ttggtgcttt tacaaaatct gctcagtttc cattttatat ttggctacca gatgcaatgg
     4201 aagcacctac accagtcagt gcttatcttc attcagcaac aatggttaaa gcagggttat
     4261 atttaatcgc tcgaatgaca ccaatcttcg cagcatcgca aggttgggta tggactgtca
     4321 cactcgttgg tttaattaca ttattctggg cttcgctaaa cgcaacaaaa caacaagact
     4381 tgaaaggtat actggcattt tcaacagtat cccaattagg tatgattatg gcgatgcttg
     4441 gaatcggtgc cattagttat cactatcaag gcgatgatag caaaatatat gctgctgcat
     4501 ttacggctgc aatattccat ttaatcaacc atgcaacatt taaaggtgcc ctatttatga
     4561 ttacaggtgc agtggatcat tctactggta cacgtgatgt taagaaatta ggtggcttac
     4621 taacaattat gcctatttca tttacaatca ctgttattac agcattaagt atggctggtg
     4681 taccaccgtt caatggtttc ttatcaaaag aatcattttt agaaacaaca tttacagcaa
     4741 gccaagcaaa tttattcagt gttgatacat taggctacct attcccaatc attggtatag
     4801 tgggcagtgt cttcacattt gtatattcaa ttaaatttat aatgcatata tttttcggac
     4861 aatataaacc tgaacaatta cctaaaaaag cacacgaagt atcaatatta atgcttttat
     4921 caccagctat tttagctaca ttagtaattg tatttggttt attccctggc attttaacta
     4981 attcgattat tgaaccagct acatcttcga ttaatcatac ggtaattgat gatgttgaat
     5041 tccatatgtt ccatggatta acgcctgcct ttttatcaac attagtgatt tatattttag
     5101 gtatcttact aattgtgaca tttagttatt gggtgaaatt gttgcaacga caacctggta
     5161 aactaacatt caattattgg tacaacagaa gtgcaaatgt tattccaaac tactctgaaa
     5221 agatgacgaa tagctatgtt accgattatt cacgtaataa tttagttatc atttttggtg
     5281 cattaatttt attaacattt gtcactatat tctcagtacc tttcaacatt aattttaaag
     5341 acgttagtcc gattagaatt ttcgaagttt gtatcgtaat tttattatta agtgctgcct
     5401 ttttaattct tttcgctaaa tcaagattat ttagcatcat tatgttaagt gcagttggat
     5461 atgctgtgtc tgtattgttt atattcttta aagcacccga cttagcattg acacagtttg
     5521 tcgttgaatc tatttcaaca gcattgttct tattatgctt ctaccactta ccaaatttaa
     5581 atcgttataa tgaaaagcgc tcattccagc ttactaatgc tttaattgct ggtggtgttg
     5641 gtttgtccgt cattattatc ggtttaattg catacggaaa tagacatttt gaatcgatat
     5701 ctaagtttta tcaagagcac gtttatgatt tagctcacgg taaaaacatg gtaaacgtca
     5761 tacttgttga tttccgtggt atggatactt tattcgagtc atctgtgctt ggtatcgcag
     5821 gattagctgt atatacaatg attaaattac gtaaaaagcg tcaaacgcaa ggtaacgagg
     5881 tgaaaaatca tgaatagaca acaaaatgat ctaattttgc aatttgctgc agttattatc
     5941 tttttcatgg taatggtctt tggtttttca cttttcttag caggacatta tacacctggt
     6001 ggcggtttcg tcggcggatt attattcgca agttcacttg ttatcattac gattgcattt
     6061 gatatagaaa caatgcgtaa aattttccca ttagatttta aaatcttgat aggtatcgga
     6121 ttagtttttt gtattgcgac gcctatcgca agttggtttt taggcaaaaa tttctttacg
     6181 catgtcacat ttgatatacc gttatttata ttagaacctg ttcatatgac aacagcagta
     6241 ttctttgatt tcggtgtttt atgtgcagta gttggtactg tcatgacaat tattatttcg
     6301 attggagaga acgaatagtg gaaattatta tgatttttgt tagtggtatt ctcacagcaa
     6361 ttagtgtcta tctcgttttg tctaaaagtc tgatacgaat tgttatggga actacactat
     6421 taacacatgc agcaaattta tttttaataa ctatgggcgg acttaaacat ggtactgttc
     6481 caatttatga agcgaacgta aaaagctatg ttgatcctat cccgcaagca cttattttaa
     6541 cagcaatcgt tatcgccttt gcgacaacag cctttttctt agtattagca tttagaacat
     6601 ataaagaatt aggcacagat aacgttgaga gtatgaaagg agttccagaa gatgattgaa
     6661 agtaatatgc ttgtgttaac gctagttatt ccagtaatta cagccatatt acttgtcttt
     6721 attggaaagc gtcctattat aaagcgttat gtcgcactag gcggtacatt attaaccttg
     6781 gtcgcggcaa ttatcaattt agcaaatgtt gttaaacatg gaccaatacg cgtagagcta
     6841 ggttcatgga aagcaccata cagtattgtc tttgtattag acatattcag tgccctacta
     6901 ataattacta gtattattat tacagctatt gtgattttat attcatacca aacaattggt
     6961 attgaacgtg aaagatatta ttattatttt tcagtactat ttatgttaat aggtatcatt
     7021 ggtgcattca caactggtga cattttcaac ctattcgtat atttcgaagt atttttaatg
     7081 tcatcttatt tcttattagt aattggttct acaaaaattc aactacaaga aacaataaaa
     7141 tatgttcttg ttaacgttgt atcgtcatcc ttcttcgtaa tgggtgtagc gattttatat
     7201 tcagtagtag gaacattgaa tctagcagat ataagcaata aattagctaa cctttcagcg
     7261 catgacagtg gcttagtcaa tatcgttttc atactattta tctttgtttt tgctacaaaa
     7321 gctggcgtat tcccaatgtt tgtttggtta cctagtgcat actatgcacc accaattcca
     7381 atcattgcat tctttggtgc tttgttaaca aaagttggtg tttatgcaat cgctagaaca
     7441 ttgagcctat tctttagtga taatgtaagc ttttcacatt atgtcatttt attcttagca
     7501 ctattaacga tcatctttgg ttgtgtcggt gctgttgcat atgccaacat taaaaaaatc
     7561 atcctataca atgtcatgat tgctgtaggt gtgatcttag tcggcgttgc aatgatgaca
     7621 gagtcaggta tgattggggc tatttactat acattgcacg acatgttagt aaaacttgca
     7681 ttattcttgc ttatcggaat tatgattaaa attacaggca ctgctgattt aagacaattt
     7741 ggtggcttaa ttaaacgata tcctgtactt ggttggtcat tctttatcgc agcattaagt
     7801 ttagctggca ttcccccact cagtggcttt tatggaaaat tttttattgt ccaatcaaca
     7861 tttgaaagag gattttattt aagtggtgtc atcgtcttat tatcaagttt agtcgtatta
     7921 tattctgtca ttcgcatatt cctacaaggc tttttcggtc aaccaaaagg atatgatctt
     7981 aataacaaag tcgacgtcaa atatttaact acgattgcaa tcgttgcagt tgtgataact
     8041 gttttatatg gattgtcagc agattactta tatccaatgg taaaagctgg tgcagaaaca
     8101 ttctacaatc catcaaccta cgttaaagct gtattaggag gtaaataaca tggctgtaca
     8161 attagtatta aactttatta tcgcagtgtt ttggttgttt gtgacaaata gttacacaac
     8221 aaataacttt gtgttaggtt tcatctttgg cctagttctt gtttacctct tacacagagt
     8281 gttaccaggt agattttatg tcatcacgct atatcgtatt attaaattag ttattatttt
     8341 cttaatcgaa ctgattaaag ctaattttga tgttttgaaa attattatta aaccttcgat
     8401 taaaaatgaa ccaggattct ttgtttatca cacagattta aaaaaagatt ggcaaattgt
     8461 cttgctgtct aatttaatta cattaacacc tggtactgta gtgcttggtg taagtgacga
     8521 tcgtacaaaa atttatattc atgccataga tttttcaact aaagagcaag aagtagaaag
     8581 tattaaaaca tcattagaga aaattgtaag agaggtgggc gaaatataat gaatcataat
     8641 gttattatcg ttattgcatt aatcatagtt gtcatttcta tgttagctat gctcattcgc
     8701 gttgtgctag gcccatcact tgccgatcgt gttgtcgcat tagatgcgat tggtcttcaa
     8761 ttaatggcag ttatagcatt attcagtatt ttattaaata ttaaatacat gattgtcgtt
     8821 attatgatga ttggtatatt agctttttta ggtactgcag tattctctaa atttatggac
     8881 aaaggtaagg tgattgaaca tgatcaaaat catactgatt agtcttgcac ttatctttgt
     8941 tatcatcggt gctttaatta gcgccctagc agctatagga ttattgagac ttgaagatgt
     9001 atattcacgt gcacatgctg ccggaaaagc atcaacatta ggtgcaatgt cattactatt
     9061 tggtacgttt ttatatttta ttgctactca aggttttgta aatatgcaat taatcgttgc
     9121 gattatcttt gtattaatta caggtcctct ttcaagtcat atgattatga aagcagcata
     9181 taatattaaa acgccttata ctaaaaagac taaagtcgat gaaatttcgg aagacttaaa
     9241 agacacaaaa ttgtagatta tataacaaaa tcatgaatat aaatcaacaa caaacagcag
     9301 taagatgatt ccaaattagg aatgatttta ctgctgtttt cttttgacat tgttacctct
     9361 ttttcaatga ttttttcttt gactacagat tcgccctatc tacatatatc tctttaattt
     9421 aattgccttt catgtcgtta tgtattatga taataataat tataaatcgt aacgattacg
     9481 ttttaaaaag agagaggttt tattatgcat tggacaatta tcggcggtgg catacaggga
     9541 actgcaatcg cacaaaaact attatcaagc ggattaacaa cagaccgatt aacaatcatt
     9601 gacccacacg aaactttttg ccaaaggttt aactcatata caaatcgaat agaaatgcct
     9661 tatttaagat caccgattgt acatcacgta catccacaac cgttccatct aaaacaattc
     9721 gctaaacagc accaatatac aaatgctttt tatggtcctt atcaacgacc tgaattgaca
     9781 atgtttatgg atcatattgc acatgcttct aaacaatatc aattagagga ttgcttggtt
     9841 caaggtttag ttcaaacttt agataaacaa gaagacaaat ggcatatcaa gttagaagat
     9901 ggacaaatta tcactacaga ttgcgtcgtt attgcaatag gcagtacaaa tattccgttt
     9961 atgcctgaca ttttaaaaga caaacagaat gtaaatcata tcttcgagaa agaacttgat
    10021 caagtagtat atgataagac cgatcatatc gttggtagcg gcattactgc tgcacatctt
    10081 gcacttaaat tgttaaatca tgataacgat aaaaagattc atttatggct aaataaagat
    10141 attgaaatac atgactttga tgctgatcct ggttggttag gtccgaaaaa tatgtcttca
    10201 tttttaagta ctaaaagcat gcctgaaaga aatgccattg tacaacgcga acgtcataaa
    10261 ggatcaatgc ctcacgaact gtacttacgc cttaaaaaac atattaaaaa tggtcgtata
    10321 aatgtgcata aaacacctat cactcaaatt agtggtggtg taattaacac tgaaaatgat
    10381 tctgttccat atcaacagat tatggttgca actggttttg aacaagattt tatgtcacaa
    10441 ccacttatta agcaattaat acaaaattat gatgcaccta tcaacgaatg taattaccct
    10501 gttatttccg aaaaattaga atggatacca aatctatttg tcgcaggatg ctttgcagac
    10561 ttagaattag gaccatttgg tagaaatgtt atgggtggcc gtaaagctgc cgaacgcatt
    10621 gaacaagcat ttctaaaact acaacaatat agcgcataaa aaaactgcta gccgtagtta
    10681 tgaatatctc ataacatttc agctagcagt tcttttattt taaaggttta atagcaactg
    10741 taccacgcat aactgtaatt aattgttctt tgtcattctt aatttttata tcccatacat
    10801 gtgtcgactt acctcgatga ataatttcag ctgtcgcagt aacacgacca tctttagcag
    10861 aatgaatatg gttagcattc atctctaaac ctaatggaat aaatttggtt gtatcaatta
    10921 aattagcaga tcctaatgaa catgctgttt cacctaaagc aatcgaagcc ccaccatgta
    10981 aatatccaaa tggttgtttt actttatcag taacaggcat agaaataaca actaaaccat
    11041 cttcctggtg atctattgac atctcaaatg tctctaataa atgagtcata ttttcacctc
    11101 tttatgctta tagtaccata gcagcaatag taccgaaaat cattaaagga atattataaa
    11161 acaagaagtt tggtacacat gtatcacgta tatgatcatg ttggccatca acatttaatc
    11221 ccgcagttgg tcctaatgtt gaatcacttg caggcgaacc tgagtcacct aatgcactcg
    11281 ctgttccaat caatgcgatt aatgccattg tatctagtcc aattgacgct ccaaaaggaa
    11341 tgaataatga tgcgataata ggaattgttg caaatgatga tccaatacct aaagtgacaa
    11401 ttaaacctat cacatacatc atgataatgc taaataattt attatcacca gtaatacttg
    11461 ttaaagtttt aactaattca tctatatcac cagtagcatt cattacacca gcaaatccat
    11521 ttgctgttaa aataactaca ccaatataag ccataatttt aataccttca acaaacttag
    11581 catctaattc ataccaatta tatgcacgtg aaataaagaa tacgagtacc cctgccagtg
    11641 caccaaaaat cattgaatct gtaaatgttt gtactaaaaa tgtagctagt attgctacaa
    11701 ttgttactat taagatatat ggttttaact ctgtaacatt gtcactatct gaaattttac
    11761 gtgtttcata ttcacgtggt ttacgatata catataaacc gataagtaag ccaacaatat
    11821 accccattga aggaataagc attgctttcc aaatcatatt aaactcaatt gggtgatttg
    11881 cctttgcaaa gccactttga ataatttgct ggaaaatttg accgaatcca tatggtaata
    11941 acacatacgg gaaacataaa ccaaaaccga taatcaaacc gattaaacgt ctatctattt
    12001 ttaagtcatt aaacagactt aacaatggtg ggatgacaat tggaatgaat gcaatatgta
    12061 caggaattaa gttttgactc atgatactca tagctaataa tgcaatgatt attgtcactt
    12121 tgactttaac tcttgaccat cgactatttt cagcgtgaat tgcatttata atttttccta
    12181 ctaaatagtc tgtgatacca ctgtatgaaa ttaatgctgc aaatccacct aataaagcat
    12241 agcttaatgc tacctcagca ccatcgacta tatttttccc aaatacattt ataacttttt
    12301 caatgctcat gcctgaaatt aagccaccaa ctagcgcact gataaataag cttataacta
    12361 cgtttaatcg acataaacat agcacaatca ttaaaattac tgctattact actgcattta
    12421 tcatatgaca accatccttt atcactctat cgaactaaag cgtttataga ctacatagta
    12481 tcagtagctt taacacctgt caatagcaaa ctataccaat aatctttatt ttttagttaa
    12541 ttgtctttct taaaataatt ttagctttca ttaaattaaa caattttaca agcttggaac
    12601 accaatcaaa atcctaagtt ctaaaatgca atattagtag tcgttgactg aatgaacata
    12661 tgcttataat atttttttgc aatgctagtc aagttgattt atgctcacaa ggatatgcga
    12721 tttatatttt cttacaacaa tgaaaatgcc tgatacaatg cgatccttta tactttcaag
    12781 agttaaatta gaaatgtata tcaagacaca agttgtatca ggcaaataat taaattattg
    12841 ttgttttaac cattgtacta tcgtcggaat cataaatcct gttggccctt ttggaccatt
    12901 atatgaagct ttattagtcg ttgctggacc tgcaatatcg aaatgaatgt gaggtgtttg
    12961 accactaaaa tgtgttacaa aacttgccgc aaatagcgct ttaccttgtc cattcgtatg
    13021 gttaactaaa tcagcgatat cactgtgttt aatacttgca cgttcggttg cagtaatcgg
    13081 taattcaaat accatttcat cgacttcaga acttatttgt aatatatcgt ttaatatcac
    13141 tttactattc gattcaaatg cagcagcttt atcatcgcct agtgcaacaa ttgctgcacc
    13201 cgttaatgta gcaaagtcca taatcacact aggctgatat tgatttgcat aaaacacagc
    13261 atctgcaagg actaatctac cttcagcgtc tgtattcatt acttctacag tttcaccact
    13321 taatgctgta aatacatcat ctggcttcat tgatgcttca tttatcatat tttcagcaca
    13381 cgcaagcact ccgacaatat ttacaggcag ttgtaaacga ctagccgctt caatgatacc
    13441 aacgacattc gcagcgccac acatgtcaaa cttcattgta gccatgccat tcttcgtttt
    13501 aatactataa ccaccagaat catacgttat acctttacca actaaggcaa ttggtgcttc
    13561 atctttgtct ttgccattat atgtgatggt tactaatctc ggtttatgct tactaccttt
    13621 acctactgct tgtaaaagtc cgaatccttc agaaactaaa gtgtcataat ctttaacatc
    13681 tactttgacc tttgtatttt taaaatgatt aacaatatct tctgcaaatg tttgtggtgt
    13741 taatacattc ggtggcatat tactaaagtc tcttgccaaa ttaatggatt ggccaatact
    13801 gataccctca tgaataaaat ctaattcaat taatgattca ctaataaggt ttaaattcgt
    13861 cttaaacggt gccttcttac ttgatttata atgatcgaat tcatatgttg cacgctcact
    13921 ttgaataccg catgccatta atacatctga taactgatca tattttgaaa taaatgaatc
    13981 cataagtaaa tacgtatctt caatgtgttc tgactttatg tattggaaaa gatgtcccca
    14041 tattttcaac atatcttgat aactacgtgt ctttaagttt cctaaaccaa ctgtaattaa
    14101 acgatatgtt tgatcttgta catcaaatgc agttgtataa atcttcccaa ctttactacc
    14161 aataatatgt tgatgtttta gtctttcaag tgattctgta atatcgatat gattaaaact
    14221 aatgcgctct aactgattta aatgttctgg tataccaata atcaatgtat ttatttcgtt
    14281 gcttagtgtg ttatttaatt taaaattcat aatgtacctc cttagatttg atatgttact
    14341 cacttcaaat tgtacaacaa taaagccctt cagtgacact gaaggacttt ataaatgaat
    14401 aatttaattt tatgtgttta gctgaaactt tatttaaagc ctagaattta ccttttttga
    14461 atgctaaacc gataccaccg attttgaata ccgcacgtgt atcaatcact tttttcatga
    14521 atgctgcttt tttaccagcg ataggtttac caaataccat acctacaccg tcatgtgaac
    14581 ctaaagaaca aacagttcca cgatcaacgt attcgaattc ttcagttgat tcaccgttta
    14641 agatgcgttt aatgttttta gcaacacttt caccttgttg cattgcaatt tgtgctgtag
    14701 ttggtaatgg acgttcttct ccagctggga taaacgctga acagtcacca ataacaaaaa
    14761 tgttgtcgta accattgatt gttaaatctt gctttgtaac gatacgtcca cgtttaacgc
    14821 cttcaaatga ttcttccatt aatttactac cacgtacacc agctgcccat actgaagtac
    14881 ctgcatttaa ttgttgtttt tcaccatcta cttcaactac aaaacctttt tcgttacaag
    14941 caacgattgg tgtagcaatt ttaaattcaa caccgcggtc ttctaagtag ctaactgcgt
    15001 ggttaactaa ttcttctgag aacattggta acattttagg tgctgcttca acacaagtga
    15061 ttttaacttt attttgatcc acaccatatt tgctacataa ttcaggaatt ctgtctgtta
    15121 attcacctaa gaattcaaca ccagtgaatc cagcaccacc aactaagata gataaatcgt
    15181 tatcatcttt ttcttttgat gctgcatagt tagcaaattt gtcttcgata tgacgtgata
    15241 attcacgtgc tgtgataaca ttttcaattt ggaaagcatg atctttcata ccttcgatgc
    15301 cgaatgtttc actaacgaaa cctaatgcta ctactaaaat atcaaagtca taaatacctt
    15361 gatttgtttc taccttttta gcatcacggt caatttttgt tacttctgct tgaacaaagt
    15421 tcactttgtc tttcttcaag acactttcca caggatataa tacatcttca tagtttagtg
    15481 tacctgctga tgcttcatgt aaccatgttg cttcatagtg atattcattt ttattaataa
    15541 gcgtaatttc tgcttcttct gttgatatcg ctttttgcaa tttagttaca gtttgtaaac
    15601 ctgcataacc agcaccaagt acaagtactt ttttacgatc ttgagccatt taatttcacc
    15661 taagctttca tattttttta accaaatgct gataattact agtaacaact ggtcattact
    15721 acttttacca caatgtatac tagtttatca tcattttgtt atttaagtct tttaaattat
    15781 gtaaaaacct gtgatgttca caaaaaagac atttttaatc caatactaat tctatagctt
    15841 ttatgttata ttttcaagca agattcagtc agattgtgaa aaaattaatt aattttttcg
    15901 tagcctgatt tcgattaaaa atgtatccgc tatcacgatc atttaaaata ctatttatag
    15961 tcagattctt aattttcaat aagcttttta ataaaacaaa atatctttac gcaaaaatag
    16021 gacataatca aatatgtcct taatcgttat tgatatcaat ttgtatccca aaaattgggt
    16081 taaccattaa aaagttattt tattagcaat tttcaggatt acctgcaact tttgcagttc
    16141 taaatgaact accacagcca catgaagcaa ttgcattagg attgtcgatt tggaaaccgc
    16201 cacccattaa tgattgctta aaatcaatag tcgtaccatt taatacgggt gcatcttttt
    16261 tgtctactaa tacttttaat ccaaagtatt ctaagacttc atcattttca ccaggcgctt
    16321 cttctgcacc cataccgtat gttaaaccag tgcacccgcc accattcact ttaattttta
    16381 aatagccatc tggcatttca tttgctttaa gcatatcttt tacttcgtaa gcagctgctt
    16441 ctgttaatat aactgttggc atgataactc ctccttaaaa aatccaagtt tcttttatat
    16501 gtgcatatat attttgtaat aattcttccg gcgaatcacc ttcaacaata tcaccattta
    16561 ctaaagcata caacccggct gaacatatac cacaatgtgt caggcaacca tactctaaca
    16621 catcgacatc tgggtcattt tccagttgat taaaaacata atctccacct tttgccatgt
    16681 tagagagaca aaattctacg atcggattca tacttcacct tcttatttca tttgttacaa
    16741 tattatagca ttttaaaact ggtattttaa catgatgtgc tcaattagca acaactgatg
    16801 tttcttatcc cagttatgta atagtgcctt agttagtaca aatatttatt tgtagtcatt
    16861 ttcaaaaaaa tatataataa gcatataaat acatatttac ttttttaggg gttattgatg
    16921 aatcaaagac acttgcattt tataactgtt cattaacaaa tatatctatg cactttgatc
    16981 ttttcaaaat taaagtaaat attaacatat aaaaggggta cttcattatg aaaaacttag
    17041 ttttgttagg cggcggatat ggtaatatgc gtatcatgtc acgcatttta actacttctt
    17101 taccacaaga ttatacagtc acattagttg atcgtatgcc atttcatgga ttgaaaccag
    17161 aattttatgc tttagctgcg ggcacgaaat cagataaaga tgttcgtatg aaattcccta
    17221 atcatccaca agtgaataca gtttatggtg aaattaacga catagattta gatgctcaaa
    17281 ttgtctcagt cggtaattct aaaattgatt atgatgagct aatcattggt ttaggatgtg
    17341 aagataaata tcataacgtt ccaggagccg aagaatatac acatagtatt caaacactct
    17401 caaaggctcg ggatactttc catagtatta gtgaactacc agaaggtgct aaagtcggta
    17461 tcgttggtgc tggattaagc ggcatagaac ttgccagcga attaagagaa agtagatcag
    17521 acttggaaat atatctttat gaccgtgggc cgcgaatttt aagaaatttt ccagaaaaat
    17581 taagtaagta tgttgcgaaa tggttcgcca aaaataatgt taccgttgtt ccaaattcaa
    17641 atattaataa agttgaacct ggtaaaatat ataactgtga tgaacctaaa gatattgatt
    17701 tagttgtatg gacagcagga attcaacctg ttgaagttgt tcgtaacttg ccgattgata
    17761 taaatagtaa tggacgcgtg atagttaacc agtatcatca agtaccaaca tatcgtaacg
    17821 tctatgtagt tggtgattgt gctgatttac cacatgcgcc aagtgctcag ttagccgaag
    17881 ttcaaggtga tcaaattgcc gatgtgctta aaaagcaatg gctaaatgaa ccattacctg
    17941 acaaaatgcc ggaactaaag gtacaaggta tcgttgggtc attaggagat aagcaaggtt
    18001 ttgcatatat catggaccgc actgtaacag gacgactcgc ttctatttta aaatccggcg
    18061 tactttggtt atataaatat cacaatggtt aaaattaaaa agcaatgtca gttcacacta
    18121 ttactgacat tgcttttagt tatgtgtata ttattcattc acaagctttt ggtctataaa
    18181 tcgagtgatt tgctttgttt gtatataacc atctgctaca tattcatcgt tcattgtaat
    18241 taatggataa aatagttcat cttgttctat tctttcaata aattgtaaat catgatctgt
    18301 taagttgtca ttatcttttg taatatctat atacgtatat ttaaatgata tatttgggta
    18361 ttttcttttt aatagcggct gtagccagtc ataaatatct ttcgatgttg gcgcattaac
    18421 gcaacttgca catataacat ctgccccata aactaccaca ctcacgtgct ccatatattt
    18481 atcccccatt ggtttgatag atttttatta cactatctat tataatatac taataaagat
    18541 tatcaaattc atctctcgaa aggagacttg cctgatgcct actgaagata caacgatgtt
    18601 tgatcaagta gcagaagtta ttgaacgtct tcgtccattt ttattacgtg atggtggcga
    18661 ctgctcattg attgacgtgg aagacggtat tgttaaatta caattacatg gtgcatgtgg
    18721 tacatgccca agttctacaa tcactcttaa agctggtatt gagcgtgcat tacacgaaga
    18781 agtgcctggt gtaatagaag tagaacaagt attctaatat aaagatatta agttaacaga
    18841 acatattatg aataacaaca aagacaggaa tactgaatta tttataatca gttattacct
    18901 gtcttttttc gctgtcaaat tggactgatg ggaaagttat tttatactat ttatctgatt
    18961 aattatttta ttattgccac atatccaagc acatattact attctattct atgaaattta
    19021 aaatataaaa atagcacata cgctcaatca aaatcgttga gttatgtgct atttgtattt
    19081 taatttttag gtttatctac ttcaggttgt ggttcacctt tcatatgttt cgcaatttgc
    19141 tcatccatat aaacccaacc tttccaaccg atgtgtacgg catcactgat aacatatttt
    19201 tcatattctt tatcagtcat atcgtaaatt ttaccaccat tatctacaac agtagaatgg
    19261 atttttttat aaactgcttg acgacgttct ttatcgatac caatgtggtc ataccataca
    19321 ccgtttgatg gaatacttac atattgaaca tctgcaccag cagcacgcat tgtttttaca
    19381 agtaattcta aatcttggaa ttctggagaa ttaacattga attcgtagtc acgtctaact
    19441 ttacgcttac tttcttgaat taatttccag tattgatctc taataccaaa tttattcgat
    19501 gtagtatcag ctttaccaat ttcaactgct ttttgtttca tctcatccca agaagcgtct
    19561 ggttttgtag caggtttaac atgttctaat ggagatttat ccattgcaaa caatgattta
    19621 atcgcttcta ttttaatcaa ttgattctct ttaaaaccag aaatataact atctttagtt
    19681 tctttagggt ttttagcata agatttcaag tattctttat tgtgtacatg tggaaactgt
    19741 aataaacgtt gtgcataacg acgttttaat tcagtagaca tgtttttctg ctggaacatt
    19801 tgattaattt gagtttgaga catacgagca tcaaagtttt gattcgttaa accatggttt
    19861 gtaaaccatt gtggtgaaat aataaatgtt aatttcttac cttttaattt atcatactgt
    19921 gatgcaagtt caactgcgtt aattaagtct gtagaaccac cagcacctaa taagaatgct
    19981 tttttgttgg cgttatgctt atttaatgca attgcaggat taaatgggtc atctttacct
    20041 aattcactag agccgtatat aggataatac ttgtttgatt cgtataactt atcttgaatg
    20101 agtgtgcctt ttagtacttg atctgtcaat gaagttctat tatcttctac agtcttttca
    20161 tttactaatc ctgtaaacca actagcaggt aataatagaa agacaatgaa taccgctcca
    20221 ctaattaaaa tgggtaaaaa aggttttaat ttcatcgtaa ctcttctaat gcttcaacga
    20281 ttttatttgg tgttgcccac tcatctctat caaagtccat aatagatact tcgatatcaa
    20341 gtttattttg aatctctaat aataatccaa ctgtttggaa agaatcaata ataccttctt
    20401 caaaaatttc tacgtctgga ttttctttta caatatcatt ttctgctact tctgctaata
    20461 aatttaatac ttgttctcta aattccataa ttaaattctc ctttattata taagtttacc
    20521 tgagaagatt aaaaagccaa atgttacaaa gtggaatgta atcacaatgc taagtgctgt
    20581 tgtgaaacca ttttgccaac gtggcggatg tttcttacgc caacgttcat aatagccata
    20641 acctataaac aatgctgcat ggtataaacc ataaacaatg taatacactt cgataccatg
    20701 ccaaattccc attatgaaga agttgattaa gaatgccacg ttagacattg caaattgact
    20761 cttcaataat tttttacgag acatgtagaa taaagatctc atgtaaatac aatctctgaa
    20821 ccagaatgat aatgtcatat gccatctatt ccagaaatct ttaatatttt tcgctttgaa
    20881 aggtttatcg aagtttggtg gtgttttaat accgaataaa taactaaatg ctatcgcaaa
    20941 taaactataa cctgcaaagt caaagaataa atataagctg tatgcgtaca tatataacca
    21001 caaattgaca aagccatgta agtctaattg taacggcatg attgcatatg tgttaataaa
    21061 gtaagcaaca atatatttat acaagaaacc aagcataatc atgtgaattg cttttaatac
    21121 taattcacga tattcattgc ctgttggtac ttttttatcg tctttaacga aacgtttgta
    21181 acgatcgatt ggtccagatg aaatcgttgg gaagaatgaa ataaattgaa ttaatttcca
    21241 tactttaatt tctttgatag aaccatcacg aatttccata attaactgca cacttttgaa
    21301 tgtaacataa gaaattccta agaaaccaac aaattcaatt aatttacttt catggaagtg
    21361 aatctgatgt ccacctaacc atgtactttg taacactttc acaagtgcta atggcaatat
    21421 tgataaaacc attacagtta caaattttga aaatgaatta ttttttggtt ttgaatgata
    21481 ataaaacatt atcaataaaa cttgccatac gacgtaaata ataaaactaa ttaattgaac
    21541 acttaaatac ttttggtcaa acagattatg tttatcagaa gaaaagatta acacaatcat
    21601 aaatgctgta actacgccat tataaatgta acttcgctta cctaaaaatc caagtataat
    21661 gactggtaat aatgcaatta aagcaattaa gaagaatgta aaatcaccat atggaatcat
    21721 ccgttaatta cctctgcaat tttctttctg tcaattttac catttgaagt caatggcaat
    21781 tgttccatcc attcaaactt tctaggaatc atatactctg gtaagcgtga tttcaagtca
    21841 tttttaatat ttttagtcat ttctgcattg tccgtaactt cagtcgttgg cacaattgca
    21901 ccaattaaat gaataacttt atcattttta tatacaggta caacaatcgc ttcttttacg
    21961 aactcagact ggcgtaattg tgtttcaatt tcttctaatt ccattctgta gccattcaat
    22021 ttgatttgga aatcaatacg accttgaatg aaccattgac cattttcaaa cttcgcttta
    22081 tcaccagtgt gatatgtacg aataccgtca tcgaaattaa atacttcagc tgttttttgg
    22141 tcatttttta agtatcctaa acttacactt tgaccttcga taacaagttc accttcatct
    22201 gtagtagata atcttgcgcc tggtctttca acgccaacag gtaatgtcgg atattgatct
    22261 aagatttctt gtgtaatttg aatacttgta actgctaccg tagcttcagt tggaccatat
    22321 gtgttgtaaa tcgtcgcact tgggaaacgg tttactaacg cttttgctgc tctgtgaggt
    22381 agaatttcac cacagaagaa gaattcgtta agactaccat attgttcttc attaagcgtt
    22441 ggtaataata aacacatttc cataaatgat ggtgttgata cccaaatgtt aatcggtgtt
    22501 gctgttagca tttcatttaa taatttaggt ttattaatca tgtttttatc tacaagattt
    22561 aatgtaccgc ctgatgctaa acatggataa atagccatta cagataaatc aaatgaaaat
    22621 ggcgcttggt taagccattg ttgttcattt cctgatttat taagttctaa catccactca
    22681 gtaaattgaa ctaaacttgc atattcaatt tgaacacctt taggttcccc agtagaacca
    22741 gatgtaaaga ttgtgtatac tgtgtcgtta tctttaatct gactatcaaa aattactggg
    22801 tcttgagatg ttttaatatc ttctattgta aatacttcgc cttctaaact ttcaaatgat
    22861 tcatcagtcg tattaaatac aaactctggt tgaaccttgt taataatcat tttaatacgg
    22921 tcttcaggaa ttgaagtgtc tacaggtaca tatccacatc ctgctttaat ggcaccaatc
    22981 atcccaacaa tcatatatgg tgacatgtga ccgaataaaa tcatcggttt cttactacct
    23041 tgtaatcgat gtgctaattt actagactca tccattaact gttgataagt taattcatca
    23101 gttgtgtgtc taacagcaat gctttgtgga tttgcatccg caaacgcttg cagcttgtta
    23161 ataatatctg tcatattaag tctccctcat tagaactcat tataaatgaa gttattgtgt
    23221 gtgtcgccac tgccataaat taaatataaa gtaataaata ttgccaaata caatagtgtt
    23281 aataaatatg gtttgaatgc ttcaacatat ttattaggtg gctgtttact tttagatttc
    23341 atattgcacc tcttaaagtt cttagtaaaa acgcctttat aaagaccgtt caatataaaa
    23401 tacgttttaa aatttgtttt ttacaattca ttatatcgat attcataatg aaattcaatt
    23461 ttaattttat agattcaaca tagtaattgg ttgtcatcac tcaattattg taaatgatac
    23521 actttgttga gaacatcatt cattttaaag gttatttaaa caataaacaa ttacagtcta
    23581 tataacaatt ttgttatata cgtcaaaatc aaataaactc atcacattaa tatgacgagt
    23641 ttataatgtt attgaattat catcagcgca aatatataca ttcgcaagtc aagcataaca
    23701 tatttaacaa ttgctttgct tgttttacca atgattaaaa accatactta ttttcaattt
    23761 actggagtat gtggtacctg atttgtcata accgcttcta tattattaat acataattga
    23821 atcatattgt cccgtgttgt tactgatgcg ctaccaatgt gtggtgtaat cagaacatta
    23881 tcacgtccca ttaatggatg tgtatgatca atcggttcat ttgctaatac atctaaacca
    23941 catgctaaaa tttctttatt gtctagtgca tcgattaatg ctgtttcatc tacaatttgt
    24001 cctctaccga tattaataaa aattgcatca tttttcattt gttcaaatgc ttcagcatta
    24061 aatttatgat gtgtttcttt tgtaagtggc gctgtacaga tgataaaatc actttctgct
    24121 aacaacgttt caaaagaaac atatgttgca ttaaagtccg cctctgcatc tttatgtctt
    24181 gatcgattat gataaagaat attagtatta aacccttgca accttcttgc aaaagcttta
    24241 ccaatatctc ccataccata tattccaata gttgaattga agacatcttt acctgacaat
    24301 aaataaggac cccagctttg ccatgcatct gcttctacat atttttcagc ttctacaata
    24361 cggcgtgcta tagcaagcat taatgtaaat cctaattctg cagttgtttc agtaagtaca
    24421 tttggtgtat tcgtgacaac cacgttattc gctgttgcac tttcaacatc gatgttgtca
    24481 tatcctacag ccatattcgc aattactttt aaatttggtg attgcgctaa aatttctgca
    24541 tcgatctgtt cacttaatgt aataaaacaa gcatctgcgt cacgaagtgc tgtcacaaat
    24601 tgatctttag gcataggcac taatgatttt tgccacataa caacgtcacc aagtttactt
    24661 aattgttgat aaaatttatc tggaattttc ctcgaaacaa ctatttttac caaatcaatt
    24721 cacctacttt ataaaatgac tatatctatt tttctaattc agctattgct tcatttaaat
    24781 ctttaaaaga atacgttggt ggcacatttt tattttgcac atcttctaac gtagatacac
    24841 ctgtttgtac atgaatcgta tccataccta cgttaatacc agacataata tcggtatcgt
    24901 acaaatcgcc taccatagca acttcggatt tatctaatcc taaaatttct aatgctttaa
    24961 ccataataat cggttctggt ttaccaataa attgtggcga tacacctgtc gatacactta
    25021 caacacttgt aatagcacca ttaccaggta ataaaccacg ctctttagga attgatacat
    25081 caggatttgt agaaataaat gttgcaccat ttcttacacc taacgttgca atcgcaagct
    25141 tttcatatgt aacttgttcg tcaagtccaa taactacata atcaacatgc tcgtcatttt
    25201 taatgacaag tcccgcttcg gttaacgcag tatttaaacc actcccacct aacatatata
    25261 ctgatgctcc tggtgattgt tctgaaatat aatcagcagt ggctaacgct gacgttacaa
    25321 cctcttctgg tttagcatca atgtgcattt cacgtaattt ttcagttact tgctcaggtg
    25381 tttttgttga attattagtt acgtataaat gaggaatgcc ttttacattt aaataatcga
    25441 tgaattgttt tgctccatca atctcatctg ttcccatata cattgtgcca tctaaatcga
    25501 ttaaatacgc tttatactgt ttcattattg attttctcct ttaccaaaag ctgttactgg
    25561 tacattttct tgatgtaaaa atgtcgttac ttctgtaata aattgtttgt aataaggtaa
    25621 tgcgtcatca aacattggca tgataatctt aatatctaag gctgtgtaat catatgtaaa
    25681 ttgtttacga ataccgacag ttttattaat cgcctgctgt gtttctttag taataacatt
    25741 ttctagttct aatatatcaa tcacatcttt ataattacca ggatccctta aaataaatgc
    25801 atcgataatc atattcccta tatctaccga tgattctatc aacatttgag caatgcgttc
    25861 aaatgcataa tgattgtgct tgctctcatg ataatcatca gttaatgctt gtaaataggc
    25921 taatttctga gttagtttat ctttgtctac aaaatacatt ccttgtcacc tctcacttat
    25981 atattatcat aattttgcag tatacttcat ttgtcgttat actaacttta ctacttttaa
    26041 aaggagtcgc tacaaaatga ttgatatgta tttatatgat gacaacgaag agagtcaagt
    26101 tcagtttgtt ggttttgttg gcgagcatag tcgatatgat ttgatgttag tccatacaaa
    26161 tagacattat ggtaagacac tcgtacttaa tatgcaaaca aataaattcg gtattatcgg
    26221 tactgatgat ttaaaagaag aaggctatat cgctcatatt ttaggtgtaa atgctgaaga
    26281 aggcgatgaa attactgagt atttaaatga agtcattcat taaagataaa aacccctaac
    26341 tagattacag gtccatgctt gaattgcatg attcccattt tcaatctagc taagggtttt
    26401 attaatgttc agtatcttga atggttggct taatcttcac atcttgaagt ttattgtcgt
    26461 catctatcac atcagacgga tgaacttctt cgattaactt tttaacctct tgtttagaca
    26521 aatgtcttaa tacaaagtaa ggacaaccaa agttacaata ttcaaatatg taatcttgaa
    26581 tatttgaaaa acgtttactc atctctgctt ttttattaga atctttgtaa aaacctttta
    26641 atcgtaattg atcgtaacca tagtcaccaa ctatgtaatc atatttatct aaaatatctg
    26701 aatacctagc aataaattgt tcttcattaa aacattcgcg ataattttct attaattcaa
    26761 agtaatgttg atctactttt atcaaattct tcacctatct atgacaatga aaggctcagt
    26821 ttatactttt agtgtttcat aaaaataatg attacttcta tgacacgttc gttttgtaca
    26881 aactaagcct ttatgcctta ttaatggtta aatattaact atttaactgt gcctcacctt
    26941 ggcgttgctt ttctttagca gcttcattta cttgctcatc cgcatgataa gaactacgta
    27001 ctaaaggtcc agcttggcaa tgtttaaacc ctttatccat tgccactttt cttaatttac
    27061 caaactctaa aggcgtgtaa tatttttgaa cctttaaatg tttacgtgaa ggttgtaaat
    27121 attgaccaat cgttaaaata tctacatcat tcgcacgtaa atcatccatc gtttcataaa
    27181 tttcttctat agtttcacct aatccaacca taatacttga tttagttggg atatccggtt
    27241 gtaattcttt tgaacgacgt aaaaactcta atgttctgtc gtaagtcgca cgcgcacgaa
    27301 ctctcggtgt taagcgacga acagtttcaa tattatggtt taaaatgtca ggtcttgacg
    27361 ccattaatgt ttctaacgca tcatagtccc cgcccatatc tgatggtaaa atttcaatcg
    27421 ttgtaaatgg atttctttct ctaactttac gtactgtctc agcataaaca tttgaaccag
    27481 catctcttaa atcatcacgc gcaacagcag tgataacaac gtgtttcaaa ttcattaatt
    27541 caactgattc agctacacgt tcaggctcat ttaaatcaag ttcattaggt aaacctgtct
    27601 taaccgcaca aaaacgacaa gctcttgtac atacggcacc taaaatcata aatgtcgctg
    27661 tacgacgtgc accccaacat tcatgtatat taggacattt agcttcttca catacagtat
    27721 taagattttt ttccctcatc atcttcttaa gtcctgtata gttttcgttg gtatttaatt
    27781 ttattttcaa ccaatccggt ttacgtaata tttcctcgtt ttttgtcgcc ataacaacac
    27841 ataccctcct gttaagtgta tctttaaaca ttatatcgaa attcatgaca aatgaaaatc
    27901 caataatctt actttttaat attccaataa tttttcttta aatatatctc ttaaaaactc
    27961 tggcattaaa tactcttttg gtaattcttt caatgttggg aaataacgac cgaacgtata
    28021 catatctatc gtacctagta catattcttt gtcgtcttcg atttcttctc cattcagata
    28081 gtaacgccct gtagaatgaa tgtaccctaa attataaaga atatatcctc caaatatatt
    28141 ccctctgaaa cccaaacctt gtgcatgttc atacatatat tcttgtttat tacttttagc
    28201 tataatctct tttaatttca caccaaaaag tctaaccctt accatattaa tcggatgcgg
    28261 taacatttga tgaatgtcat attctgtcac ttcatctttt acaatatctt taacgagtaa
    28321 gccagcattg atgatggcac attgtgcatg tgtatactca caaacacttt gagctaataa
    28381 gtatgcagct tcagttatgt gattcatact acgcttaagc actactggat gttgaattac
    28441 tgaattggac attaacgttt ttccttcttc ttcaaatgaa gtttcaactt caggtaatgt
    28501 ttctaaagga ataatctttg cagttttatg tactacttta tgtgcctcaa aagttaaatt
    28561 aacctctcca agataattac catactttcc agctgccgcc atcagtacac cattattgat
    28621 ttcaccatgt tcaaaataat gatgcgtatg actaccaaaa atgacatcaa tttcaggcaa
    28681 ttcttggcat aatgtttcat cgaagaaaat gccacaatga cttagcacga ttaatacatc
    28741 aaatttacct cgttgaagtt caatttcttc ttttatagat tcaagtggat cggtaacaat
    28801 ccaatttagt gcacgataaa atggggtaaa aggtgctgtc gctgcaacga atagtatttt
    28861 cacaccgtct atgtccttaa tataagaaga aacgatatta tttggtaaat gacctgattc
    28921 atctataaca ttgctacatg tcactataaa ttttgcttcg tcgtaaaggt gatttaaagc
    28981 ttcgtgtgaa atggtcatcc cttcattatt accgattgtt gcaacatcac attttgcttc
    29041 atttagtaat gccacattct ttttacctaa agttgcttca gttataggtg cggataaatc
    29101 tacatgatca cctagatcaa catataaaga aggatgatta agtcgtggcc gatgttctgc
    29161 catatatgct ttaatgcgtt cgtattcatg taaatgacta tgaatatcgt tcgtatgata
    29221 aattgtaagc ctcaaattga cgtcctcctt ttaaagttgt attaaatcaa cgcacgaata
    29281 attaaatata tacccattaa caacattact gttctcaata atgttactac cgtatctgac
    29341 ttaattgatt gattcacttt gacaccgatt ttcgcaccaa aataactaga aataattaaa
    29401 atgattgcat aaccccaagc tacgtgacct tgagcaatgt gccctataga actcatgaca
    29461 cttgaaaaga aaatcatcat catacttgtt ccaacagcta catgaggtgg aaatctaaat
    29521 acaataagca ttagtggcgt cattagtgcg ccacctccaa taccaaataa acctgtcaat
    29581 ataccaatta aaaacgttgt aataaaagca aacaatggtg gaacactata atgatatgtt
    29641 ttacctttag cgtctacata agtcttttca tacttgggtt tatcgaaaat tttaaacggt
    29701 ttaatcttat ttcttaccat taataaaatg gctacgaaaa ttaaaaagat accaaaatat
    29761 aaattaaatg actcaaatgt taaatatcta ctaatgaagg acccaagcaa tgaacctggt
    29821 aatagtccaa ataaaaagat ggaaccattt ttaatatcaa cttgttttgt ttttaaatat
    29881 ccaagtgatg aagaaagtcc tgtcacaatt agaatgactg aagacgtccc tatcgctact
    29941 tgtgttgtaa tattatgtag taatccatgt tcaacaccga ggtaaaccat tgttggaacg
    30001 ataataatac cgcctccaat gcctacgata gaccctataa tcgctgacaa acctccgatt
    30061 aaaactaata atgtaattgt taataacata gtcgtcgcct cttaaaataa ttttaattgt
    30121 tgtggtgcta acccttcata ttctatattc aataatcgct gatatgtttt ggcattattt
    30181 gctgcatgac caccagaatt attgttaaat atgacgtaaa ctttcttagc tttttgtgct
    30241 aatatttgtg ccttttgtgc caagtctatt aattcttgct cattataatc atataaatag
    30301 cgtacatcgc gccattcttg atctgacata tctttcttag tccaaccgta atgattacgt
    30361 ccatgataac gtacaaacgc aatttcattt gtgattcgat tgactaaagg tacactgcca
    30421 tctttcactt gtggttcatc aactactgca tgaatgattt gatgttctgt taaaaatgcc
    30481 aatgtttgtt ctttaaatgc atcactaaac catgattgat gcctaaattc tacacacatt
    30541 ggaaatgctt gtaattgctg tcttacatac aaaatatatt tgatattttg tgcattgcag
    30601 tcaaaccatg gcggaaattg aaccaatacc attgctaatt ttttctgtgt atgtaagggc
    30661 tctaacatat tcttaaattg atcaaatagt tcttgccttg tatctgcaaa tgttttgtag
    30721 tctgcatgca atgtgagtgc ttgatgaatt ttgaccacaa attcaaatgt atcaggcgtt
    30781 tcttttatcc atttcaatat atttctttcc ggttgtatcg catagtatgt cgcatctaat
    30841 tcgacaaccg gaaaatgtcc agcatatgtt ttaagtttat cggtttggcg ttctaaatct
    30901 tcatataatg aatagtgatc accccaacct gttaatccga tgtttatcat atatatcacc
    30961 aatgtcatca taccatataa cttttatcat aatcatttca gcgaacttta gtttgatgtt
    31021 tttgcttgat taaaatcttt cggcgatctt cagcttgatg ttttcgtttg attaaatttg
    31081 taaaatagaa acgcaatcca caaaaatggc aagcactaaa ataatgtttg ggggtgcttg
    31141 tgcttttgtg gattgcggtc gattatttat attgcatgat ttgattaatt tgattgatta
    31201 tattggacat gatggtgttg gcgggatgcg ttgttgctag tcgcgggctt tgtccactcc
    31261 acatatgtat taactctttg tcgccgatgt ttgctgcggc ttttcttatg ctacttgtta
    31321 gctcattttg tattggataa tctgggatat cgccttcgta ttgggacatt tcttcgataa
    31381 acctattgtt gataccgcgt gcaagctttc cactaaacgc ttttgtaatg actgtatctg
    31441 tttctttact atttataatt gcatctcgca gtagttctga tgcattactg tcttgtgatg
    31501 ttaaaaatgc ggtgcccatt tgtacccctt ctgcacctaa gacaatactt gccaaaactc
    31561 ctctaccatc cataattcca ccagcggcaa tgaccggaat tgaaacgaca tctacaattt
    31621 gtggcactaa agatattgtt ccaaccatag gtaattgatt tttaggtttt aaaaatgaac
    31681 cacgatgtcc acctgcttca ctaccttgag caacgatagc atccataccc gctttttcat
    31741 tcgcaatagc ttcatcaaca cttgttgctg tacctataag tttgacattc gctgctttca
    31801 acctgcttat aatctgttcg cttggaattc caaaagtaaa acaacataca ggcacttgct
    31861 ttttaattat cgtatcaata tgacacttaa attgttgttc ttcggtaatt tttacaaccg
    31921 gctcttctaa atgtaatgcg cgtcgataag gttttaacca tgcattcata ttttcaattt
    31981 gactactggt atatgattgt tgacttggta caaagacatt tacgccaaaa gaatttgacg
    32041 ttaattggcg tacataatct atttcatctt ccaattgctg cgtattaaag taacctgcgc
    32101 ctattgtgcc taacccacca ctgttactta ctgatgcaac taatttcggt gtcgtacttc
    32161 ctgccatacc tgcttgtata attggatatt caatacttaa catttgagta agtcgattct
    32221 tattccacat agctgttcgc tccttatata gatacgttgc gatttttccg ttgttgaaat
    32281 tgaatttgct gttgagaaag tttttctttt tcctttttat ccatctcatc ttcaatttcc
    32341 atacctaata attcttcaat taagtcttca tgtgacacta tcgcttcagt accaccaaat
    32401 tcgtccaaca caattgctaa atgttttcta gaaatagtca tcttacgtaa tacccattca
    32461 gctttattgt gttcattcac aaataatggc ttagctgaat agtttgtaat ttgattttct
    32521 tttttattac tccaagccaa cagatattta gaatgaaaca ccccaataat gttatcaata
    32581 tctccctcgt acactggata tctagtgtat ggcttattca taaccgtttc ataaacttct
    32641 tcgtatgtcg catttgaagc aaatgccgtc acattaattc taggtgttgt atctacatct
    32701 tttactttta aattttcaaa attaatgaca ccttccaacc tactcgtctc aatttcattt
    32761 aaagcacctt catgtccagc aattgctaac attgttttaa attcttcttt tgaaaattga
    32821 tgttcttgag gttggccctt agataaactt cgattaatac tgtccgtcaa cttatttaaa
    32881 agtaatgtga taggacggaa cacaatgaca caaatattaa taattggata tacaagcctt
    32941 gttattttat ctggaaatgt tgcagcgaca gacttgggaa tcacttcgga gatcaaaatg
    33001 ataacaactg ttaaaacagc tgatgcaata ccaacgctaa tcccccaacg taaagccata
    33061 attgtaacaa gtgttggtaa taaaatattc gcgacattat tcccaattag aatcgttgta
    33121 ataaactcac ttggtttttc aagtaacttt acaatgcctt ttgctttttt atcacctttg
    33181 tcagcttcag ttttaaattt tgttttattg gcagccgtta atgccgtctc gcttcctgaa
    33241 aagaaaaacg aaataaatat caatataatt atggcaatga tcacgcgtgt agtctcctta
    33301 ttgtcatatc ttatttttat tgtagttact taattcccga cgttctcgtt taataaacca
    33361 gttaaaattc attatatata atagtcttaa taatgataat taatatgcat ttttagtcat
    33421 agttacttta atgcattcat tagtaaaatg atgctatagt tcgagctgtt tgttattaat
    33481 aaatttgcat aatgaaaaat ttcaacaaat tcagtttatg acatagttat aatcatttga
    33541 aaccattata atgataacaa ccagtttctc ataaatttat tctaattatc ttttaaataa
    33601 ctttagacaa gtacttgttt atatccaaat atagaaagaa ggattaacat gtattttgtt
    33661 ttagcaatat ttacaataat cagtgccagt gtaagtttag gttattcaat tcaagcatgt
    33721 gcatctagcc ataatataaa tgcatattat gcacttagtc gaagcttacc tttattttta
    33781 ttagctattt tttctttagt cattcatagt gctatatttt tgataactat atccattgca
    33841 atgatcttag ttcaattttt agatgcgatt gttggttata aaagtgaaga tgtctttaaa
    33901 acttatggtc cattagcaac atctgtagtg aacttaatat tattaatagt tttcttattt
    33961 taactcactt atacaacgaa tcttaatcga actaatatcg aattatctaa agaatatttg
    34021 atgtagcttt caattaattt aataaagacc agcactctaa tgccacaatc atgttgtatt
    34081 tgtgttgtcg ctttatccac catcaatgat tattttttac accaatcaaa aaatcgaact
    34141 gatataaata agtacaaagc ttatctatca atccgattta gttataaaac aaaaaaagcc
    34201 acagtaatgt ggctttttgt tatattcagt atcaaaatgg tatcaatagc cattttcgga
    34261 agtcaagaat ggcttaacaa cgcggtttaa agctatccaa tactaccttc catttcgaac
    34321 ttgattaaac ggttcatttc gaccgcgtat tccattggaa gttcttttgt aaatggttcg
    34381 atgaatccca taacaatcat ttctgtcgct tcttcttcag aaataccacg actcattaga
    34441 tagaataatt gttcttcaga aacttttgaa accttggctt catgttctaa tgatatttga
    34501 tcgttgaata cttcgttata tggaattgta tctgatgttg attcgttatc taagattaat
    34561 gtatcacatt caatatttga acgagcacct tttgctttac gtccaaaatg aacaataccg
    34621 cgataaataa ctttaccacc atttttagaa atagatttag aaacaattgt agaagatgta
    34681 ttaggtgctt tatgaatcat tttagcaccg gcatcttgaa cttgtccttt accagcaaat
    34741 gcaatagata atgtactacc ttttgcacct tcacctaaaa gaacacagtt tggatatttc
    34801 atcgttaact tagaacctaa gttaccatct acccattcca tatttccgtt ttcataaaca
    34861 aaagtacgtt ttgtaactaa attgtataca ttgttcgccc agttttgaat cgtagtataa
    34921 cgaacgtgcg catctttatg cacaatgatt tccacaacag cagagtgtaa agaactagtt
    34981 gtataaactg gtgcagtaca accttctacg taatgtacag aagcaccttc atcagcaatg
    35041 attaatgtac gttcaaattg acccatgttc tcagagttaa tacggaaata agcttgtagt
    35101 ggcgtatcta gtttgatatt tttaggtaca taaatgaacg aaccacctga ccatactgct
    35161 gagtttaacg ccgcaaattt gttatctgct gcaggtacta cagaagcaaa gtattttttg
    35221 aataattctt cattttcttg taaagcacta tctgtatctt taaagataat acctttttct
    35281 tcaagttctt tttccatatt atggtaaaca acttcagatt catattgagc agaaacacca
    35341 gctaaatatt tttgttcagc ttcaggaatt cctaatttat cgaaagttct tttaatttct
    35401 tctggcactt catcccatga acgttcagct tgttctgaag gctttacata gtaagtaatg
    35461 tcatcgaaat tcaattctga taagtcgcca ccccattgag gcattggcat tttataaaac
    35521 aattttaatg atttaagacg gaaatctaac atccattccg gctcattttt catgttagaa
    35581 atttctctaa cgatattctc agttaaacca cgttctgatc tgaaaatgga cacatcatcg
    35641 tcgtggaatc catatttata atccccaaca tcaggtgctt ttttagccat ttcaatcact
    35701 ccttaaaaat atatttacaa cggcgtgcgt aagtttttaa ttcatgatgt aaaccatatt
    35761 ataacaatga catgacatct tataaaaatt tttatacttt tatatgtcta atatcaaaat
    35821 tatctatgat taacagcatt ctattcttct tcagtcgtac cttctgcttt accttcttta
    35881 gcaacagtac ctttttccaa tgctttccaa gctaatgtgg cacatttaat acgagctggg
    35941 aattgagata caccttgcaa tgcttcaata tctcccattt cttctgtaat cacatagtct
    36001 tcaccaagca tcattttcgt aaattcttgg ctcatttgca ttgcttctcc aagtgaatga
    36061 cctttaacag cttgtgtcat catcgatgca cttgccattg aaatcgaaca accttcacct
    36121 tcaaacttag catcttttat aatgccgtct tctatatcaa atgttagtcg tatacggtca
    36181 ccgcatgtcg ggttattcat atctactgtc atagacccgt tatctaatac acctttattt
    36241 ctaggatttt tataatgatc cataatgaca gatctatata attgatctag attattaaaa
    36301 ttcataagag aaaaactcct tcgtttgttt caaggcattt attaactgat caacgtcttc
    36361 tttcgtgttg tatatataaa aactcgctct agctgttgaa gacacattta accatttcat
    36421 taacggttgc gcacaatgat gcccagctct aaccgctaca ccttctgtat ctacggctgt
    36481 agcaacatcg tgtggatgta catcttgtaa attaaacgtt attacacctg cacgacgatc
    36541 ctttggcggg ccataaattt caattccttc aattgcagac atttgctcat aagcatatat
    36601 cgttaattct tgttcatatt tatgaattgc atcaaaacct atgcgttcta aatagcgaat
    36661 agcttctgca agcccaattg cttgagcaat taatggagta cccgcctcaa atttagtagg
    36721 taaatcagcc catgttgcat catacttact tacaaaatca atcatgtcgc caccgaactc
    36781 aatcggttcc attttttgta gtaactcacg tttaccaaat aatacgccaa tacctgttgg
    36841 tccaagcatt ttatgaccac taaaactata aaaatcagca ttcatttctt gcatatcaag
    36901 tttcatatgt ggtgctgctt gcgccccatc aacactgata attgcaccat gttgatgagc
    36961 tatttctgca atggttttaa catcattaat tgtaccgagc acattagata tatgtgcaat
    37021 agcaacgatc tttgttttat cattaatcgt ttgcttaata tcctcgatgt ttaattcacc
    37081 gtcagctgtc attggtataa atttcaatgt cgcattttta cgctttgcta actgttgcca
    37141 aggaacaata ttggcatgat gttccatttc agtgacaaca atttcatcgc cctcttcaac
    37201 atttgcatca ccatagctat gtgctacaag gttaatcgac gcagttgttc cgcgtgtaaa
    37261 aatgatttct tcaaaatact tcgcattaat aaaacgacga acggtttcac gggcattttc
    37321 ataaccatca gttgccaatg atcctaatgt atgaacacca cgatgaacgt ttgaattata
    37381 acgcttgtag taatcttcta aaacatttaa cacttgcaca ggcgtttgac ttgtcgctgt
    37441 tgaatcaaga tatgctaaac gtttgccatt gactttttga tctaatatcg gaaaatcctt
    37501 gattacttca ttaacgtcaa atgagtgttc ggccacttca ttcacgacct ttcttaaata
    37561 aaaatcctaa tcatttaaat actgacgttg tattagtctt ataccaatat cgacagtcta
    37621 tatctattac aaacttttat tttcaaaata ttatttagaa actttgcgtt caattacttc
    37681 tctcaattga cgtttaacgt cttcgatagg taattcacgt actactggat ctaagaaacc
    37741 atgtataaca agacgttccg cttctctttg agaaatacca cgactcatta aatagtaaag
    37801 ttgatctgga tcaacacgac ctactgatgc agcatgacca gcttgtacat catcttcatc
    37861 aattaataaa ataggattcg cgtcaccacg agcatgttca gataacatta atacacgtga
    37921 ttcctgatta gcaattgatt tagttccacc atgcttaatg tagccgatac cattaaatac
    37981 agacgatgca tgttctttca taacaccatg tttaaggata taaccatctg tttctttacc
    38041 atattgtacg attttagatg ttagattaat tttttgttcg cctgtaccta caactactga
    38101 tttaagtgaa cttgttgaac gatcaccaaa taaatttgtt gtattatcaa taatttggct
    38161 accctcattc attaaaccta gtgcccaatt aattgaggca tccgcttcag taataccacg
    38221 tcgaatgata tgacctgtaa agcctttatc catatagtcc actgagccat atgtgatatt
    38281 tgaatttgca ccagcaatca cttcagaaat aatatttaat tgatttcctt caccagatgc
    38341 atttgataag taattttcaa catatgtgac ttcggcgctt tcttcagtaa cgatgataac
    38401 atggttataa aagcttgcat tttcgtcgtc gtgcaacaca acgtattgta ctggatgttc
    38461 tacaactaca tttttaggaa cataaacaaa tacgccacca ttaactaatg ccgtgtgtag
    38521 cgctgtgata cgatgttcat ctactgttac tgcatctttc ataaagtact tttgtactaa
    38581 atcactatgg ttcataagag cgtctgctaa accttcaacg ataacgccat ctttcgatgc
    38641 attatcatca acttgtgtgt acgcaatcgt attattatgt tgaattacta agtttttaga
    38701 atgatctacg tcaataattt ctcttactga ctcaggtaat tgtgataaag attgataaac
    38761 atcacctttt acatcgtgtt gtttaaaaga atcaaaatcc cattttctta attttgtttt
    38821 atcaggtttt ggcatttcta aagtttctgt taatttcaaa gcttttttac gtaattctgt
    38881 catccaagaa ggttcattgt gggctttaga ataatcaaca agttgttctt cagaaatgtt
    38941 caaaatatca gttgtcataa ctttttcctc ctacttgatg aaatttacta tttcgtataa
    39001 atgttcattt tcatcatata tagatgaaca tctcatggat actgtattaa taagattatt
    39061 cagctgaacc gaactcttct ttaacccatt catatccttc ttcttcaaga cgttttgcta
    39121 attctggacc accagattta acgactttac cagcatacat tacatgtact ttatcaggag
    39181 taatgtaatt taataatcgt tgatagtgtg taatcattaa tgcaccaaag ttttccccac
    39241 gcatttggtt aataccttta gatacaactt ttaatgcatc gatgtctaac cctgaatcga
    39301 tttcatctaa gattgcaaac ttaggttcta acatcattaa ttgtaagatt tcgttacgtt
    39361 tcttctctcc acctgagaaa ccttcattta aataacgttg tgccatgtct ttatctatgt
    39421 ctagaaaatc catgttttta tctaatttct taataaattg cattaagttg atttcttgtc
    39481 cttcttcacg tttcgcattg attgctgaac gcatgaaatc agcatttgta acacctgtaa
    39541 tttctgatgg atattgcatt gccaagaata atcctgcttt tgctctttca tcaacttcta
    39601 attctaaaat atttacaccg tctaaaagta cttctccttt agttacttca tagcttgggt
    39661 gtcccataat tgcagatgat aaagttgatt tacctgtccc gtttggtccc ataatcgcat
    39721 gtatttcatc agtgttaatt gtcaagttaa caccttttaa gatttcttta tcctcaatag
    39781 acacatgtag gtctttgatt tctaatgttg atgccatttt atattccctc cgtaaaatat
    39841 aaagttttct taactagttt ataataattt taatttgtag tcaaaaagac tttgtaataa
    39901 tgcgttcagt taattataac ttacttatac cttaatataa acaacttaaa ccctttttat
    39961 tatttttaat aactctaaag tacaactcta atccgctctc tttaaaaata taaatgataa
    40021 taagtgcaca taatttctca atggatttta tgaatttaaa atatgttatc atttcactag
    40081 gacatttgta atatggtatg atgctattta tgattttatt ccaatcgttt gaaaggagtt
    40141 attcatgcaa caatttaaat ggattaatat tctaaaaggt tttgctatgg gaacgagtga
    40201 cttggtacct ggtgttagcg gtgggactat cgcattattg ttaggtattt acaatcaatt
    40261 tatcgcttca attagcggca tattctcacg acgtttttgg ccaagtttta catttttaat
    40321 ccccattata attggaatgt tactggcaat gggatcacta agtaatcttt ttaattattt
    40381 attaagccaa caccatattc caactatgtt tttctttggt ggattaatca ttggtatcgt
    40441 tccatattta ttgaaaattt ctaactataa aacgtcattt acaactaaac attacatgat
    40501 ggttatagct ggtatcgcta tcttaattgt tattacatta atgaataatg gtgataaaca
    40561 tgctggagaa acacttactc tatcaactag tcttattatt aaatatttta ttgcaggtat
    40621 gtgtgcatct agtgctatgt tactcccagg tatttcaggg tcatttatgt tattagtatt
    40681 tggcgtctat ggtacggtca tgctagcaat atctgaagtt gttaaactta actttgcagg
    40741 tcttcctata ttacttgcag tcggatttgg cgtgcttgct ggattcatta tttcaagcaa
    40801 aattatccag tatttcctaa cacatcataa actaatgact tttgcattaa tcattggttt
    40861 tgttgttggc tccctctttg cagtatttcc aggtttacca actaatatcg tgatgtggtt
    40921 cgtttcatta gtagtattta tcatcggttt tattgtaagt ttgactttag gtcgaattac
    40981 cgctgaaaat gaataatcat tttaggggtg ataaaatatt ttcagtatct cgttaatcat
    41041 atgcaatata tgtctcgttt tgtatacata ttattggata tcattagcga gtttttattt
    41101 agtttttttc taacattaaa ataattatta ataaattttg gtatccactt ttttaataca
    41161 aaaaccacct agccgatggt tataccgact aagtggcatg tgcagtgtat taggaataaa
    41221 aagtaaaatt actcgccttt gttacctttt actttatcaa taaaatcagt tgctttttct
    41281 tttgcatttt caacgaattc tttcgcttta ccagaagctt tatcttcttt accttcgttt
    41341 tctaaatttt tattatcagt aacattacct actgtttctt taacattacc ttttgcttgt
    41401 tcaaatttac tttcgtctgc cataataaat gcctcctcgg aataattaaa tgttatatat
    41461 aatacttacc cactgaaaaa ttatctaaac attttactta aataattttt gatattgatt
    41521 tgacgtcatt ttataactag cgaaatagat tcatcattaa cttgagggag tgggactgaa
    41581 ataataaaga atcactaatg atttatgatg tattagtcac tagccatgtg aaattaaaaa
    41641 taagaataaa tgagtagcac gcatgcatat aggattttac tttatccgta atagcatctc
    41701 attcctaaat atcatataaa tacctgttta aattaaaaag cccagcaaca tcacgttact
    41761 gagccattaa tatgatttat ttagcaggaa taattagcca gattatcaag gaagttcaag
    41821 ggcaatcaaa acattgttat atatgcattt ataaaatttt caagataatt tattattcat
    41881 acccttgccc tttgtttcaa aattatgccc tttttttgcc cttgaaaaca accacactcc
    41941 taaattaata ggtggtgtgg tttgatcatt tataatataa cataaaaaca accacccagt
    42001 aactagtatg agtggcgtag cgactataac aactctatgt tatcaagata tatgtatatg
    42061 agtgatgaca aggaagatgt ctcctgtgag accaacagcc agatatatgg cctctgccgg
    42121 gctatatagt tcactcctac tatatacaca tgtaattata acataaaaaa atagacaagt
    42181 accgaagtac ctgcctaaat aacaacaaga ttaacatgtg aataatggaa ataaaaagtc
    42241 agcccgaagg ctaacttacg aatagatgaa aatttgaaca cattgctgtg tctaaaatga
    42301 ttatagcata aataac
//
LOCUS       DALAAR010000017        41907 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000017 DALAAR010000000
VERSION     DALAAR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.017"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            298..672
                     /locus_tag="O2458_000898"
     CDS             298..672
                     /locus_tag="O2458_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ3886782.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            825..1223
                     /locus_tag="O2458_000899"
     CDS             825..1223
                     /locus_tag="O2458_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ3886783.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1439..2263)
                     /locus_tag="O2458_000900"
     CDS             complement(1439..2263)
                     /locus_tag="O2458_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ3886784.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2500..3807)
                     /gene="brnQ"
                     /locus_tag="O2458_000901"
     CDS             complement(2500..3807)
                     /gene="brnQ"
                     /locus_tag="O2458_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ3886785.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4391..5281
                     /locus_tag="O2458_000902"
     CDS             4391..5281
                     /locus_tag="O2458_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCZ3886786.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5530..6579
                     /locus_tag="O2458_000903"
     CDS             5530..6579
                     /locus_tag="O2458_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCZ3886787.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6592..7269
                     /locus_tag="O2458_000904"
     CDS             6592..7269
                     /locus_tag="O2458_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3886788.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7477..8508
                     /locus_tag="O2458_000905"
     CDS             7477..8508
                     /locus_tag="O2458_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCZ3886789.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8851..9969
                     /locus_tag="O2458_000906"
     CDS             8851..9969
                     /locus_tag="O2458_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ3886790.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9944..10867
                     /locus_tag="O2458_000907"
     CDS             9944..10867
                     /locus_tag="O2458_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCZ3886791.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10878..12098
                     /locus_tag="O2458_000908"
     CDS             10878..12098
                     /locus_tag="O2458_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ3886792.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12203..13735)
                     /locus_tag="O2458_000909"
     CDS             complement(12203..13735)
                     /locus_tag="O2458_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCZ3886793.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13775..14656)
                     /locus_tag="O2458_000910"
     CDS             complement(13775..14656)
                     /locus_tag="O2458_000910"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCZ3886794.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14814..15674
                     /locus_tag="O2458_000911"
     CDS             14814..15674
                     /locus_tag="O2458_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCZ3886795.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15952..16752)
                     /locus_tag="O2458_000912"
     CDS             complement(15952..16752)
                     /locus_tag="O2458_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ3886796.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16892..17560
                     /locus_tag="O2458_000913"
     CDS             16892..17560
                     /locus_tag="O2458_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCZ3886797.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17687..19000)
                     /locus_tag="O2458_000914"
     CDS             complement(17687..19000)
                     /locus_tag="O2458_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ3886798.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            19418..21490
                     /gene="lip2"
                     /locus_tag="O2458_000915"
     CDS             19418..21490
                     /gene="lip2"
                     /locus_tag="O2458_000915"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCZ3886799.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(21732..22559)
                     /locus_tag="O2458_000916"
     CDS             complement(21732..22559)
                     /locus_tag="O2458_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3886800.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(22632..23831)
                     /locus_tag="O2458_000917"
     CDS             complement(22632..23831)
                     /locus_tag="O2458_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ3886801.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            23927..24019
                     /locus_tag="O2458_000918"
     CDS             23927..24019
                     /locus_tag="O2458_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886802.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            24133..25134
                     /locus_tag="O2458_000919"
     CDS             24133..25134
                     /locus_tag="O2458_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ3886803.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            25168..25500
                     /locus_tag="O2458_000920"
     CDS             25168..25500
                     /locus_tag="O2458_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCZ3886804.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            25501..26301
                     /locus_tag="O2458_000921"
     CDS             25501..26301
                     /locus_tag="O2458_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCZ3886805.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            26291..27235
                     /locus_tag="O2458_000922"
     CDS             26291..27235
                     /locus_tag="O2458_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCZ3886806.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            27213..28235
                     /locus_tag="O2458_000923"
     CDS             27213..28235
                     /locus_tag="O2458_000923"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ3886807.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            28550..29575
                     /locus_tag="O2458_000924"
     CDS             28550..29575
                     /locus_tag="O2458_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCZ3886808.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(29668..31014)
                     /locus_tag="O2458_000925"
     CDS             complement(29668..31014)
                     /locus_tag="O2458_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCZ3886809.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(31029..31313)
                     /locus_tag="O2458_000926"
     CDS             complement(31029..31313)
                     /locus_tag="O2458_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ3886810.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(31315..31758)
                     /locus_tag="O2458_000927"
     CDS             complement(31315..31758)
                     /locus_tag="O2458_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ3886811.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(31763..33718)
                     /locus_tag="O2458_000928"
     CDS             complement(31763..33718)
                     /locus_tag="O2458_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ3886812.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            33930..34349
                     /gene="mepR"
                     /locus_tag="O2458_000929"
     CDS             33930..34349
                     /gene="mepR"
                     /locus_tag="O2458_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCZ3886813.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            34456..35811
                     /gene="mepA"
                     /locus_tag="O2458_000930"
     CDS             34456..35811
                     /gene="mepA"
                     /locus_tag="O2458_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCZ3886814.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            35915..36355
                     /locus_tag="O2458_000931"
     CDS             35915..36355
                     /locus_tag="O2458_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCZ3886815.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(36438..37796)
                     /gene="glpT"
                     /locus_tag="O2458_000932"
     CDS             complement(36438..37796)
                     /gene="glpT"
                     /locus_tag="O2458_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCZ3886816.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            38104..39030
                     /locus_tag="O2458_000933"
     CDS             38104..39030
                     /locus_tag="O2458_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886817.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            39044..40105
                     /locus_tag="O2458_000934"
     CDS             39044..40105
                     /locus_tag="O2458_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ3886818.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVKV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            40119..40685
                     /locus_tag="O2458_000935"
     CDS             40119..40685
                     /locus_tag="O2458_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ3886819.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(40745..41740)
                     /locus_tag="O2458_000936"
     CDS             complement(40745..41740)
                     /locus_tag="O2458_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCZ3886820.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
BASE COUNT        14517 a         6477 c         7958 g        12955 t
ORIGIN      
        1 aataaatata taggaggcaa tttttagaga tacatagcaa tgtaaaggta tcaaatagtt
       61 atttcataat ttattatgat gacataattt tcagtagata gttgtaaatt aacactgaaa
      121 tagatgttta tattaagaag actttaatat cataattaat caaaatcttg cgtcaaacat
      181 ttaaacgaat tttaagttga taacaattgg caaaacattg ttcaaacatc acaatgataa
      241 agcatattat cagtattgta gtgtgtggaa aatgacagcc atctaaggag aaaaatgatg
      301 aaaagaatat tggtagtatt tttaatgtta gcaattatat tggcaggttg ttctaataaa
      361 ggtgaaaagt atcaaaaaga tattgataaa gtgtacaaag aacagaatca aatgaataaa
      421 attgcctcga aagtacaaaa cactattaaa acagacatta aacaagaaga cagtaataca
      481 catgtttata aagatggtaa agtcattgtt attggtattc aattatataa agatcgtgaa
      541 aaaatgtatt atttcgcata tgaaataaaa gatggtaagg cagagattaa tagagaaata
      601 gacccaatta agtatatgaa agaccataaa gcagattatg aagatgaaaa tgtagaagtg
      661 gaaaaagatt aacataggct ttctttaaaa cttttaacca gtttcaaaaa gtaaatgagc
      721 tttagcgaca ataattttta aaataaacac gatataaatt taaacattta tatataagaa
      781 cacaaacgca agtttgtaaa taataataat aaaggagtaa gacaatggaa aaatcgatca
      841 aaataatgac aataatagga attgttgttc agggtttagc aacggtattt agtttactat
      901 tgatggtttt agcagcatca ggtgtaatga ctacagatgt gtcaacaaca gttaatggtg
      961 aggttgaccc agttgatgca gaaacagcag cagcaatttt cactgtatta ttcttatccc
     1021 tattcatatt tggaatcatt tcaattattt taggtgcaat cggtatgttt aaagcatcta
     1081 aaaacaaaaa aatgagtggt atattgttga ttattggagc tgtaataagt ggtaacataa
     1141 ttacatttgc tttatggtta atcagtggta ttaaactact tactaataac aagcctaaag
     1201 atgaaataag cgacttatca taaacatcgt atattgaaat ttcaagattt cttaagtaat
     1261 taaataaagt gctctcttag aagtagattt tcagtcaata actgctttta agagagcatt
     1321 ttgtatttga taatgctcat agcatcggat aaaaatcatt gacgaacttt aaaaaaatac
     1381 gttagacaat gcacttctaa atattaaagg catcgactaa cgcattgttc aaatatagtt
     1441 aattattttt ataaaattga tgatgatcat tcaagtaagc atagaataaa cctataatga
     1501 gtccgcctcc aatatagtta ccgataaaag ccgcagcgat attcgaaata gctggtatga
     1561 agtgcaatgt atcaacttga taaattaaac cacccataaa taagcaactg ttgtaaacga
     1621 cgtgttcata acccataaag gcgaatatgg taacaccgaa catcatgaca aacatttttg
     1681 cgagtacatc gtcaatttgc atggcaataa ctaatgaaat attgataaag aaattggcga
     1741 agatcgcttt cattaatata cttacaaaac cagtagacaa cgttttatgc tctataactg
     1801 ctgataactg atttaacata tctggcgtca ttacatttga aaaacgcatg aaactaaata
     1861 aaatagcagc acctaaaata tttcctgcaa agcataataa aaatattttc aatactctag
     1921 ttggtttaat tactttataa tacaggccta cagtaaagta catgaagtta ctggttagta
     1981 gttcggagtt tgtaaataaa atgagtacta acgcaaagct gaatgtaatg gcactggcca
     2041 tattcacaat gcctggcggt aaatctggtt cgtgtgttgc tttaactgat aatacgaaga
     2101 ccgtaataat cccgataata aatcctgcca tcatcgcgcg taataaataa cgttttaaat
     2161 aaacgctttg taatatatct tttgttctta tcgtttcgac tacgttattt acccagtcgt
     2221 ccccataaaa aatcttatcc catttaatat gtttctcctt cacaatgaca tcctcacttt
     2281 tcatttgtta atcagagtat actatgaatt gccttatttg tgaataattt cacaaaatta
     2341 attttaaaaa ctaatatttt tgaataacag ctaggatttt ttaacaaatc atttatttta
     2401 aaataatcaa tagacagacg gtatcactga aaagtcatga atttcatata aaaaagccta
     2461 gtaacgatgg gtcaatcatt actaggcagt gagcatttat taagttgtcg cttgtttcgg
     2521 acggcgtata aatacatcga ttatgaaacc gataatagca aagagcatga atggtacaag
     2581 ccaagctaaa tcgatatctg ctaaaggtaa catcataaac gatttcaaaa taacaccgtg
     2641 taataagttg aaactattta gtatttgtaa aattgaaata atcaatgtaa taacagttgc
     2701 gagtcgatag gcccaactga atctgaatgt gctaaacatg ttagcaaatg atatgagtac
     2761 aagtgcaatc gacacgggat atattaaagt caataatggg acagcaattt ttaaaatcat
     2821 ttctaaacca agtgttgtaa ataagaaccc tatgatagag aaaataagtg cgaatatttt
     2881 ataagaaaac ttaggtacgt gtttcttagt aaatgtggcg caagcattga cgagtcctat
     2941 acatgttgtt aggcatgcaa ggataaccgt cattccaaat acgaggttac cgaacgaacc
     3001 aaataatcgt aatgagttgt acgtcaatat atctgtacca tctttaaagt ttcctggagc
     3061 tgttgatgcc ccaacgtatg caagtgcaaa gtaaatcatt ccaagtaata tggctgcaat
     3121 aagacctgaa aagcagacat attttaaaat tttcatgcga tctgtgaggc ctttaaactt
     3181 atagccattg acaatgacta cggaaaaagc taacgcagca acaagatcca ttgtaaaata
     3241 gccttccaaa cttcctgaaa tgaaaggatg tgttatatat ttatccttag gtgcacttag
     3301 tgcagattca gggttgaaaa tgacagcaat acttaataga gcgaccatta atagtaataa
     3361 cggtgttaat aatttaccta aattatcaac gattttcgat ggatttaaac taatccagta
     3421 aacgatggca aaaaagattg ctgcgaatat aattaaagtc cattggttat gcacaggtaa
     3481 aatgtgtctt gtaccaattt cgtacgcgac atttgcagca cgtggaatac cgtaaaatgc
     3541 tccgatagac atgtaaatca cgacagcaaa aataaacccg aaccatggat gtatacgatt
     3601 gcctacactt tcaacacctt catcataaaa tgcaacaaca ataacagtaa taaaggggag
     3661 taatatgcct gtaagggcaa agcctagcat accaatccac atattttgac ccgctgtatg
     3721 gccaagcatg ggcgggaata ttaaatttcc ggctccaaaa aatagtgaaa ataacatgag
     3781 gcccgaaata ataacttgtt ttttcaatgt aatttccttc ctaaaactag atattcataa
     3841 taatttaaaa aatctgaaaa ataaaaacgt tcttacttta tctttaaatg acaatactat
     3901 gattctatta ttttataaaa aagattgcaa taataacgtc ttgctattat tggataatga
     3961 caattaaagt gtcgttttta tgaattaaaa taggtgaaag taagttggaa atacgttgga
     4021 gcgttgtgat ttttatgaga aattgtatga gtgaggactg ctatttataa aaggtaaact
     4081 atggatgagt ttatggttgt tccaataggt aaataagaga tagcacactg tacacataat
     4141 gatacgtgga tgagtgaata tgtctgatag gaaggcctag tcactcaaat gaatttaagt
     4201 ttattacgtg ataaatcaca aatctctctc atgtgatagg tctcccatta aatcatgctt
     4261 atatgaaatg ttcacatatt tgttagcttt tcaagaaata atattaaata tcttttcata
     4321 gtatagcaat attaaataaa tgcatttata attttaactg tattttaaat attaatcatg
     4381 aggtgataag atgaataaaa tttcaaagta tattgcaata gcatcattat cggtagcggt
     4441 tacagtttca gcaccacaaa cgacaaattc tacagcgttt gccaaaagtt ctgctgaagt
     4501 tcaacaaacg caacaagctt ctataccagc atcacaaaag gcgaatcttg gtaatcaaaa
     4561 tattatggca gtggcttggt atcaaaattc agctgaagca aaagcattat atttacaagg
     4621 ttataacagt gcaaagacac agttagataa agagattaaa aagaataaag gtaaacataa
     4681 gttagctatt gctttggatt tagatgaaac agttttagat aattctccat atcaaggcta
     4741 tgcatcaata cataataaac ctttcccaga aggttggcat gaatgggtac aagctgctaa
     4801 agctaaacct gtctatggcg caaaagaatt cttgaaatat gctgacaaaa aaggtgtcga
     4861 tatctactat atttctgata gagataaaga aaaagattta aaggcaacac aaaagaactt
     4921 aaaacaacaa ggtatccctc aagctaagaa gagtcatatt ttactaaaag gtaaagatga
     4981 taagagtaaa gaatcacgca gacaaatggt tcaaaaggat cataaacttg tcatgctatt
     5041 tggagataat ttattagact ttacagatcc aaaagaagct acagctgaat ctcgtgaagc
     5101 attaattgaa aaacataaag acgatttcgg taagaaatat atcattttcc ctaacccaat
     5161 gtatggtagt tgggaagcta cgatttacaa caataactat aaagcaagtg acaaagcaaa
     5221 agataaatta cgtaaaaatg ctattaagca attcgatcct aaaacaggcg aagttaaata
     5281 atatatgaat tggacgtcta catgtactta aaggtatatg taggcgtttt taaattgatc
     5341 cttaatttta aatgtaccgt acaaaaataa tagacaatta attgcatttt ccataaattt
     5401 gcttttaata taaaaagttc gggtataatg aaggataagt gtatgtataa cattacagag
     5461 cgcaacacga cgtacgtgca tctaagaata taaacaatgt ttaaatcata gtagtaggga
     5521 gagaaatgca tgtttttagc ttggaatgaa atacggcgca acaaattgaa gtttggacta
     5581 attattggtg tgttaacgat gattagttac ttgctatttt tattatctgg attggcgaat
     5641 ggtcttatca atatgaataa agaaggcatt gataagtggc aagcagatgc cattgttcta
     5701 aataaagatg ccaatcaaac tgtgcaacaa tctgttttta acaagaaaga tattgaaaat
     5761 aaatacaaga agcaagctac tttgaagcaa acaggggaaa ttgtgtctaa tggccatcaa
     5821 aaagacaatg ttttagtgtt cggtgttgaa aagtcatcat ttttagttcc gagtttaata
     5881 gaagggcata aagcgactaa agataatgaa gtgttagctg atgaaacgct taaaaataaa
     5941 ggatttaaaa ttggtgacac attatcacta tctcaatcag atgaaaaatt gcatatcgta
     6001 ggttttacag aaagtgcaaa atataatgcg tcatcagtca ttttcacgaa tgacgctacc
     6061 attgccaaga tcaatcctag attgactgga gataaaatta atgcagttgt tgtacgtgat
     6121 acaaattgga aagacaaaaa attaaaccaa gagcttgaag cggtaagtat taatgacttt
     6181 attgaaaatt taccaggtta taaaccacag aacttaacat taaactttat gatttcattc
     6241 ttatttgtca tttcagctac agttataggc attttcctat atgtcatgac attacaaaag
     6301 acgagtttat ttggcatatt aaaagctcaa ggatttacga atggctattt ggcgaatgtg
     6361 gtaatttcgc agacggtcat attagcacta tttggtacgg catttggctt actgttaaca
     6421 ggcgttacag gtgcattttt acctgatgca gtacctgtca aattcgatgt actaacattg
     6481 ctcgtatttg caattgtgtt aatgattgtc tctgtattag gaagtttatt ctccatttta
     6541 acaattagaa aaatagatcc gttaaaggcg attgggtagg aggtgtagca aatgttgaaa
     6601 tttgaaaatg taacaaagtc atttaaagat gggaatcgta acattgaagc ggttaaagat
     6661 acaaattttg agataaataa aggtgatatt atagcattgg ttggaccttc tggctctggt
     6721 aaaagtacat ttctaactat ggcaggtgct ttacaaacac cgacatctgg gcacatttta
     6781 atcaataacc aagatattac gacaatgaag caaaaagcat tggcaaaagt tagaatgtct
     6841 gaaataggtt ttattttaca agctacaaac cttgtaccat ttttaacggt aaagcaacaa
     6901 tttacattat tgaaaaagaa aaataagaat gttatgtcta atgaagacta tcagcaactt
     6961 atgtcacaat taggtctaac ttcattgctt aataagttac cttcagaaat ttcaggtggt
     7021 cagaaacaac gtgtggcgat agccaaagcg ttatatacga atccgtcgat tattttagcg
     7081 gatgaaccta ccgcggcgtt agatactgaa aatgcgattg aagtcattaa aattctacgt
     7141 gatcaagcca aacaaagaaa gaaagcatgt attattgtta cacatgatga acgacttaaa
     7201 gcatattgtg atcgttcata tcatatgaaa gatggcgtcc ttaatcttga aaatgaaaca
     7261 gtagaatagt tttattaagc cggtacatca tgtgccggta tttttatgtt tatgtattat
     7321 ttgaataaac tttcacattc aattaataat aattattatc gaaaatcaga aatattccgt
     7381 gaaatataat attttttgta gtaaaatggc ctctaagtat tcaatattta aatatgggga
     7441 ttgaatataa aattatcgta atgggggtca atggttatgg atttattgat aggtacttta
     7501 tttttatttt tggtcttagt gatttttaca ttatttacat ataaagcgcc taatggtatg
     7561 cgtgccatgg gagcattagc taatgcagca atcgcaacat ttttagtgga agcatttaat
     7621 aaatatgttg gtggcgaagt attcggtatt aaatttttag aagagctagg agacgctgcg
     7681 ggaggtctag gtggtgtcgc tgccgctgga ttaacagcat tagctatcgg tgtgtcacca
     7741 gtatatgcat tagttatagc agccgcgtgc ggtggtatgg atttattacc aggtttcttt
     7801 gcgggttata tgattggata tgtgatgaaa tatacagaga aatatgtgcc ggatggtgtc
     7861 gacttaattg gatcgattgt catcttagcg ccattagctc gtcttattgc agtattatta
     7921 acgccagtag tgaatagtac attgattcga attggtgata ttatccaaag tagtacgaat
     7981 acgaatccaa ttatcatggg tatcatttta ggtggtatta ttacggttgt cggcacagcg
     8041 ccattgagtt caatggcatt gacagcatta ttaggtttaa cgggtgtacc tatggctatt
     8101 ggtgccatgg cagcatttag ttcggcattt atgaatggga cgctattcca tcgcttaaaa
     8161 ttaggtgatc gtaagtctac gattgcagta agtattgaac ctttatcaca agcagatatt
     8221 gtatcagcca atccaattcc aatctatatt acaaatttct ttggtggtgc gattgctggt
     8281 ttaattattg ctatgtcagg tttaattaac gatgcgacag gtacagctac accgattgca
     8341 ggatttttag ttatgtttgg atttaatcat ccgacgacaa ttgtgattta tggtgtagta
     8401 atggcgattg taggtgcgct tgcaggttat cttggttcaa ttgtatttaa aaaatatcca
     8461 attgttacta agcaagacat gattaatcga ggtgcagtag acgcatagca tcatcatatt
     8521 gaatagtaaa aacaaataaa acatagtaac gtgattcagt cgatgtaaca gtcgataatg
     8581 agtcacgttt ttttatagaa aaatacaaga cataaaaatg tcataattta ttgtcgacaa
     8641 atatcatact gtataaacat ttatcatttt ctcaagtacc ttttacacga tggaatgaac
     8701 ttacttttta cgaaattatg cgtattttat aaacaaatat cattgatata acggtaaatg
     8761 taagcgttta caacagaaat aacagcatgc tacgatattt ttgtaaattc actgattcaa
     8821 gtattttaag tcaatatgag gagggatgtt atgagcgatt ctgagaaaga aattttaaaa
     8881 agaattaaag ataatccgtt tatttcacaa cgtgaacttg ctgaggcaat tggattatct
     8941 agacccagcg tagcaaacat tatttcagga ttaatacaaa aggaatatgt tatgggaaag
     9001 gcatatgttt taaatgaaga ttatcctatt gtttgtattg gcgcagcgaa tgtagatcgt
     9061 aagttttatg tgcataaaaa tttagttgca gaaacatcaa atcctgtaac gtcaacacgc
     9121 tctattggtg gcgtagcaag aaatattgct gagaacttag gtaggcttgg cgaaacggtc
     9181 gcttttttat ctgctagtgg acaagatagt gaatgggaaa tgattaaacg attgtccaca
     9241 ccatttatga atttggatca tgttcaacaa tttgaaaatg cgagtacagg ttcatataca
     9301 gctttaatta gtaaagaagg cgacatgaca tatggcttag cagatatgga agtgtttgac
     9361 tacattacgc ctgaattttt aattaagcgt tcacacttat tgaaaaaggc taagtgcatt
     9421 attgtagatt tgaatttagg caaagaggca ttaaacttct tatgtgccta taccacgaaa
     9481 catcaaatca aattagttat caccacggtt tcttccccaa aaatgaaaaa tatgcctgat
     9541 tcattacatg ctattgattg gattatcacg aataaagatg aaacagaaac atacttaaat
     9601 ttaaaaatag aatctactga tgatttaaaa atagctgcta aacgctggaa tgatttaggt
     9661 gttaaaaatg ttattgtgac aaatggcgtg aaagaactca tttatcgaag tggtgaggaa
     9721 gaaatcatta agtcagttat gccatcaaat agtgtgaaag atgttacagg tgcaggcgat
     9781 tcattctgtg ctgcagtagt gtatagctgg ttaaatggga tgtctactga agatatatta
     9841 attgctggta tggttaacgc aaagaaaacg atagaaacga aatatacagt taggcaaaac
     9901 ctagatcaac agcaacttta tcacgatatg gaggattata aaaatggcaa atttacaaaa
     9961 gtatattgag tattctcgag aagttcagca agcacgggag aacaatcaac cgattgtagc
    10021 attagaatca acaattattt cgcatggtat gccgtaccca caaaatgttg aaatggcaac
    10081 aacagtagag caaattatca ggaataatgg tgccattcca gcaaccatag ccattataga
    10141 tggcaaaatt aaaattggtt tagaaagcga agatttagaa atactggcaa ctagtaaaga
    10201 cgttgctaaa gtatctagaa gggatttagc agaagttatt gcgatgaagt gtgttggtgc
    10261 tactactgta gcgacgacga tgatatgtgc tgcaatggct ggtattcaat tttttgttac
    10321 aggaggtatt gggggcgtcc ataaaggtgc agaacatacg atggacattt cagcagactt
    10381 agaagaactg tctaaaacaa atgtcactgt tatctgtgca ggtgccaaat caattttaga
    10441 cttacctaag acgatggagt atttagaaac aaaaggcgtt ccagttattg gatatcaaac
    10501 gaatgaattg ccagcattct tcactcgcga aagcggtgtt aagttaacaa gttcggttga
    10561 aacgccagaa cgacttgctg acattcattt aacaaaacag cagttaaatc ttgaaggtgg
    10621 cattgttgtt gctaatccaa ttccatatga gcatgcctta tcaaaagcat atattgaggc
    10681 aatcataaat gaagctgttg ttgaagcgga aaatcaaggt attaaaggta aggacgccac
    10741 accgttcttg ttagggaaaa ttgtagaaaa aacgaatggt aaaagtttag cagcaaatat
    10801 aaaacttgtt gaaaacaatg cggcgttggg tgctaaaatt gctgtcgctg ttaataaatt
    10861 attgtaggtg atgatacatg aatattttat tcgctatcac agggatagca tttgcactat
    10921 ttgttgcgtt tttattcagt tttgatcgta aaaaaataga cttcaaaaag acgttaataa
    10981 tgatatttat tcaagtgttg atcgtgttat ttatgatgaa cacaacgatt ggtttgacaa
    11041 ttttaactgc actaggttca ttttttgaag ggctaataaa tattagtaaa gcaggcataa
    11101 attttgtttt tggagatata caaaataaaa atggctttac gttcttttta aacgtattac
    11161 tgccattagt ttttatttct gtattaatag gcatctttaa ttatattaag gtattaccat
    11221 ttattatcaa atatgtaggt atcgctatta ataaaataac tagaatgggg cgcttagaaa
    11281 gttattttgc tatttcaaca gcaatgtttg ggcaaccaga agtatattta acaataaaag
    11341 atattattcc aagattatct agagcgaaat tatatacaat tgcgacgtct ggtatgagtg
    11401 ctgttagtat ggcaatgcta ggttcatata tgcagatgat tgaacccaag ttcgtagtta
    11461 cagcagtaat gttaaatatt tttagtgcgc ttatcatcgc cagtgtaatc aatccctata
    11521 aatctgatga tactgatgtt gaaattgata acttaacgaa atccacagaa actaaaacat
    11581 tgaatggaaa aacaggaaaa cctaagaaag ttgccttttt ccaaatgatt ggtgatagtg
    11641 cgatggatgg gtttaaaatc gctgttgtag tagccgtaat gttgttagca tttatttcat
    11701 taatggaagc aattaatatc atgtttggta gtgttggttt gaactttaaa cagcttattg
    11761 gctatgtgtt tgcaccaatc gcattcttaa tggggattcc atggagcgaa gctgttccag
    11821 ctggctcttt aatggcgact aaattaatta caaatgagtt tgtagcaatg cttgatttta
    11881 aaaatgtcct gggtgatgta tcagctcgaa cacaaggtat catttcagtt tacttagtaa
    11941 gcttcgctaa ttttggtacg gttggtatca tcgtaggttc aattaaaggc attagtgata
    12001 aacaaggaga aaaagttgca tcctttgcaa tgaggttgct acttggttca actctagctt
    12061 caatcatttc aggatcaatc attggcttag tattgtaaat gaatcgaagt acctaaatta
    12121 aattcatggc aaagctaaac cccgtcacca agttggcgca acagcgcatc ataacttagt
    12181 gacggggttt tatcataaca atctactttt tcgtagccgt ttttgaaatg tatgttgatg
    12241 gtttatcttt ttcaaaaatt gttaatcccg ttatatcttt tttatgtttt gaagggacaa
    12301 tgaagctaag tatataagca aagacaaaag caactgtaaa tgaaatggta gatacataga
    12361 aaggtgagtt acctttgcca acaccattat agacataagc aaagatgata cccaatatta
    12421 atccacaaat aacaccgaat gtattcgtac gtttagtgaa aataccaact gcaaatacac
    12481 cagccaatgg aacgccgaat aatccagtca caaacaagaa taaatcccat aagtcatttg
    12541 aattagaagc aattaagtat agtgacattc caaaaccgaa aatacctgca atgataataa
    12601 tgaaacgtgc aaagttaact tcgtgtcgct cgctaccttt tccgaagaag cgttgcttaa
    12661 tgtcgattga aatacaagca gatatagaat ttaaactaga tgaaatggta gactgtgcag
    12721 cggcgaaaat ggctgcaata agtaatcctg ctacaaatgg tggcatctca gtcaaaatga
    12781 aatatggcac tacagatgat gtattgaagc cttttggtaa aacagcttca tgtgtataaa
    12841 atgaatacag cattgtaccc ataccataaa ataagggtgc tgaaattaaa gctaggatac
    12901 catttgtcca taacgattta tttgtttctt ttaaactatc agaagcttga taacgctgca
    12961 cgacgtcttg actcgctgtg tattgataca agttgttgaa aatatttcct aggaaaataa
    13021 ttggaatggc agctgccgca gtatttagtt tccaattgtc tgcactaatt aattttttgt
    13081 gctcaatcgc atctgcaaag acagtgccga aaccgccttt aatgttcaca acacctagaa
    13141 taataataac taaagcgccg cctaataaaa tgacgccttg aatgaaatca ctccaaacca
    13201 caccttcgaa accacctaaa aatgtatata aaatacatag taaaccaacg agtgatgcaa
    13261 cgatataagg gttcatgtct gatacagatg tgattgctaa tgttggtaag tagataacaa
    13321 ttgcaacacg ccctaaatgg taaacgacaa ataataatga gccaatgaca cgtatgctag
    13381 ggccaaatct agcttctaaa tattcatatg cagatgttac ctttaacttt ttaaagaaag
    13441 ggacatagaa ataaataagt aatggaataa ttgcgacgat agcaatgtta ccagcgatat
    13501 atgaccaatc tgttaaaaat gctttctctg gtgtcgacat aaatgtaatc gcacttaacg
    13561 tagtagcata aattgaaaag ccaactaccc aagatggcaa gcgaccactt gcggtaaaga
    13621 aactattggt actttggctc gcgcgcttgg taaaataaac gccaatgaac aacatagcta
    13681 gtagataaat gataacggca acccagttta gtgtgccaaa tccaacttct ttcatgggca
    13741 acatcccctt tacaatgtat tgattctttg atgtctataa atcgtatttt gcaatgagtt
    13801 gatctaatgt ttgtcgatgt gcttcgttaa aaggtttgaa aggtcttttc ggtaatcctg
    13861 catcaatgcc acgatgacgt aatatttctt tcaatgttgg ataaatcccc attgataaca
    13921 ctgtttcgat aatgtcgttt gaatcatgtt gcagttggta agcttcttga atttgacctt
    13981 gtcgtgctaa gtcgaagatt tttcttgcac ggcgaccatt aacgttatat gtagaaccaa
    14041 ttgcaccatc tacgccagaa atcgtagctt gaactaacat ttcatcaaag ccagataaga
    14101 ttaatttgtc tgggaatgct tttctaatac gttcgagtag gaagaagttt ggcgctgtat
    14161 atttaacacc aacaattttt tcatgattaa atagctcgct gaattgttca atagaaatat
    14221 tcacacctgt taaatctggt attgcataaa taatcatatt gttctgagtt gcttcgataa
    14281 tatcgaaata gtaatctcta atttcttcaa aagtaaatgg atagtagaat ggtgttacgg
    14341 cagaaagtgc atcataaccg agttctgtgg catattttcc aagttcaatg gcttcattta
    14401 aatctaacga acctacttga gcaatcaatt tcactttatc cccaactgcc tctttggcaa
    14461 ccttgaaaac ttgcttcttc tgctctgtat ttaataaaaa gttttcgcct gagctaccat
    14521 ttacataaag accgtctaat tcttcagttt caatggcatt ttgagcaatt tgtttaagtc
    14581 cttgttcatt tacttgacca ttttcatcaa aaggaacgag taacgctgca tataaacctt
    14641 ttaaatcttt gttcattatg aagtccctcc aaaaatcatt tgataatata gtttacagct
    14701 ataattgtaa acgctatcat aaaatgtaac aatatctttt tgaaaattgt agtcatattt
    14761 atgtataatt aatgaaaatg tttttcaaaa tcaatagaaa tggagtgagt aaggtgtatt
    14821 acatcgcaat cgatattgga ggcactcaaa ttaaatcggc agttattgat aagcaattga
    14881 atatgtttga ctatcaacaa atatcaacgc cggacaacaa aagtgagctt attactgaca
    14941 aagtatatga gattgtaaca ggatatatga agcaatatca gttgatccaa cctgtcatag
    15001 gtatttcatc agcaggcgtt gttgatgaac aaaaaggcga aattgtatac gcagggccaa
    15061 ccattccgaa ttataaaggt actaatttta agcgattatt aaaatcactg tctccttatg
    15121 tcaaagtaaa aaatgatgta aacgctgcat tactaggcga attgaaatta catcaatatc
    15181 aagcagaacg gatcttttgt atgacgcttg gtacaggcat tgggggtgcg tacaagaata
    15241 atcaaggtca tattgataat ggtgagcttc ataaggcaaa tgaagttggg tatttattgt
    15301 atcgtccaac tgaaaataca acgtttgagc aacgtgctgc aacgagtgca ttgaaaaagc
    15361 gcatgattgc cggaggattt acgagaagca cacatgtgcc agtattgttt gaagcagctg
    15421 aagaaggtga tgatattgca aaacaaatat tgaatgagtg ggcagaagat gtagcagaag
    15481 ggattgccca aatacaggtc atgtatgatc cagggcttat attaattggg ggcggtatat
    15541 ctgaacaagg agataatctc attaaatata tcgagccgaa agttgcacac tatttaccaa
    15601 aagactatgt ttatgcacca atacaaacga ctaagagtaa aaatgatgca gcattatatg
    15661 gctgtttgca atgatagttg aaagaaggag tcattctaaa atagaatttg aaaccgttac
    15721 gagagatgag agctgttgtt agttccacac atcacactct atctaggacc aatctaaact
    15781 atatcaacca acagtgtgcc acgggcaaat taaattgaag aagctgagat attaaaattt
    15841 tagaaaatgt aaaaaaatat ttggtattga aattaaaaaa gcacctagca actcgttggg
    15901 acaatcacga tgattgtcta cagttgcagg tggatttgaa tatactacta gttatttgtt
    15961 gtctaggata atagatttag tatgttgata agtttgactc agattcgtat tttctaataa
    16021 atgataactc acgatatcga ttaaaaagag tgtcgcaatt tgtgtgttga taaattgatg
    16081 gtcggtatta cgcgattgat ccgttgttaa aagtactaaa tctgcacaat ctgtaagttt
    16141 actaccttca aaatttgtga tggcaacgac atatgcacca tgagatttgg cgacttccgc
    16201 tgcagaaatt aattccgaag tattaccact atttgacata gcaataaaca tatccgaatg
    16261 agatagtagg gatgccgata ttttcattaa atgtgaatcg gtagtaacat taccttttag
    16321 ccccatacga atcatacgat aataaaattc agtcgctgat aaaccagagc tacctagtcc
    16381 agcaaagagt atatgtcgac ttgattgaag tttgtcgata aaggtttgga taatgtcgtt
    16441 atcaataaat tcaccagttt gttgaatgat ttgttgatga tatttatgaa ttctttgaat
    16501 aattgggcta ttttcaataa ctgtctctgt catttcttgt tgaatattaa attttaaatc
    16561 ttggaaattc tcataatcca gcttatgact aaagcgtgtc atcgttgctg gtgatgtacc
    16621 aatcgcatgg gctaaggagt taatcgttga aaaggcatcg ctataaccat tttgtcttat
    16681 ataattgacg atgcgtttat cagtttttgt aaataaatgt tgataacgtt gaacacgatt
    16741 ctcaaatttc attgtgtcac cccttcatct taatgattac tattatatat gaaaaatatt
    16801 ttcaagatag taaaaagcat tgataaaaat tatcttaatg atatattgta aatgacttta
    16861 cgtgaaaaaa cgacttatgg agtgaggaat aatgttacca catggattaa tagtatcttg
    16921 tcaggcacta ccagatgaac cattgcattc atcttttatt atgtcgaaaa tggcattagc
    16981 tgcgtatgaa ggtggtgctg ttggtattcg cgcaaatact aaggaagaca ttttagcaat
    17041 taaagaaacg gtagatttac cagttattgg cattgtgaaa cgtgactatg atcactcaga
    17101 tgttttcatt actgcaacgt caaaagaagt tgatgaactg atagaaagcc aatgtgaagt
    17161 cattgcattg gatgcaacgt tacagcaacg tccgaaagaa acgttagacg aattagtatc
    17221 atatattaga acacatgcac cgaacgttga aatcatggct gatatcgcga ccgttgaaga
    17281 agctaaaaat gccgcacgac ttggctttga ttatattggc acgacgttac atggctatac
    17341 tagttatacg caaggacaat tactttatca aaatgacttc caatttttaa aagatgtact
    17401 acaaagtgtt gatgcaaaag ttattgcgga aggtaatgtc attacaccgg atatgtataa
    17461 acgtgtgatg gacttaggcg ttcattgttc agtcgttggt ggtgcgataa cacgaccaaa
    17521 agaaattacg aaacgttttg ttcaaattat ggaagattaa atgataacga taaaaaaacg
    17581 agatgaccat cattaattaa aggcacctaa ttatcttagg tggctgaatg aatgtaatgg
    17641 gttcatctcg ttttgtttgt ttatgatagt gattttattt tcaactttat ccaaaaataa
    17701 gtaaagcgac ggggatggtg attaatagcg acaacgccac gcgtaaaaac caaatgatga
    17761 tgagtttcca gacaggtatt ttaatttcag ttgctagtat acatggcact aatgctgaga
    17821 aaaagataat ggctgatacg cttactacac cgacgacaaa tttagtactc attgcagctt
    17881 tagttactaa caaagatggt agaaacatct ctacaataga aatcgctgac gcttttgcta
    17941 gtaaagcctg atcagcaatt gggaaaatat aaataaatgg atagaagata tagccaagcc
    18001 aatcaatgaa tggtgtatag ttcgctacaa tcagtcctaa aaaaccaatc gataatatag
    18061 aaggtaaaat accaacagtc atttctaaac cgtctttcaa attgtcccaa acgttcttca
    18121 cgagagatgg tgttaatgca ttttgtttca tcgcctctgc atatgcagtt ttcagtctgc
    18181 ttccttcaat agcaacttct tgttctcctt cttgtccgtt ataatattct gttgattcat
    18241 tgctgattgg cggtagccat gcagtaattg cagtcacgac aaatgtgatg actaaagtta
    18301 tccaaaagta taaattccaa tgcggcatta atcctaaagt tttagcaacg ataatcataa
    18361 aagttgctga aactgttgaa aagccagtcg caataatcgt ggcttctcgt ttgttgtaca
    18421 tcccttgctt atagacacga ttagtaatca ataatcctaa ggaataactg ccgacaaacg
    18481 aagccactgc atcgacagcg gattttcctg gtgttttaaa aataggtctc ataataggct
    18541 ccatataaac accgacaaat tctaataagc catagcccac taataaagaa agcgcaattg
    18601 cacctactgg aattaagata cttaatggca tcattaattt ttcaaacaaa aacggaccat
    18661 agttagcttt aaatagtatt gatggaccga ttttaaatac atacattata ccgatcattg
    18721 cacctgcaac tttaaataat gtaatgacca agtttgtgat tgaagtcata aaagtacgtc
    18781 tcactattgg taacgctgta ccaattaaaa tcataatcag tgcaacatag ggcataagtg
    18841 gacctatgat tgagcgaatg gctagatgaa catgatcgac gaaaatagtg ttgttaccat
    18901 taatcgtaaa aggaataaag aaacatagta tgcccactaa actatagaca aaaaaacgcc
    18961 atgcacttgg ttgttgtgca ttagaatgat attgattcat taaagcaacc cctttgttta
    19021 aatgaataca caaaactgta tgatgcatct tccccttaat gagatgaatc attattttaa
    19081 tttagaaaaa tctgaaaact tactataatt gtatagtttg aattattttc ataccaatac
    19141 aaattaacta attatatata gattgaaact atattactta ataaaatatt tatcttaaat
    19201 gttgttgtgt tgattcaaca ccacaactaa aagtgtttat aaattatttg gaaatacaca
    19261 tatttgtaaa tgattagtat cgatttaata tcgtattatt aaatttttat taattttgta
    19321 gtcttaatca aaaaataata tatgtcatgt tatattgaag gtgcagttgt ttttcattct
    19381 caagaggggg tcaaaaaaat acttttgagg tgattatatg ttaagaggac aagaagaaag
    19441 aaagtatagt attagaaagt attcaatagg cgtggtgtca gtgttagcgg ctacaatgtt
    19501 tgttgtgtca tcacatgaag cacaagcctc ggaaaaaaca tcaactaatg cagcggcaca
    19561 aaaagaaaca ctaaatcaac cgggagaaca agggaatgcg ataacgtcac atcaaatgca
    19621 gtcaggaaag caattagacg atatgcataa agagaatggt aaaagtggaa cagtgacaga
    19681 aggtaaagat acgcttcaat catcgaagca tcaatcaaca caaaatagta aaacaatcag
    19741 aatgcaaaat gataatcaag taaagcaaga ttctgaacga caaggttcta aacaatcaca
    19801 ccaaaataat gcgactaata atactgaacg tcaaaatgat caggttcaaa atacccatca
    19861 tgctgaacgt aatggatcac aatcgacaac gtcacaatcg aatgatgttg ataaatcaca
    19921 accatccatt ccggcacaaa aggtaatacc caatcatgat aaagcagcac caacttcaac
    19981 tacacccccg tctaatgata aaactgcacc taaatcaaca aaagcacaag atgcaaccac
    20041 ggacaaacat ccaaatcaac aagatacaca tcaacctgcg catcaaatca tagatgcaaa
    20101 gcaagatgat actgttcgcc aaagtgaaca gaaaccacaa gttggcgatt taagtaaaca
    20161 tatcgatggt caaaattccc cagagaaacc gacagataaa aatactgata ataaacaact
    20221 aatcaaagat gcgcttcaag cgcctaaaac acgttcgact acaaatgcag cagcagatgc
    20281 taaaaaggtt cgaccactta aagcgaatca agtacaacca cttaacaaat atccagttgt
    20341 ttttgtacat ggatttttag gattagtagg cgataatgca cctgctttat atccaaatta
    20401 ttggggtgga aataaattta aagttatcga agaattgaga aagcaaggct ataatgtaca
    20461 tcaagcaagt gtaagtgcat ttggtagtaa ctatgatcgc gctgtagaac tttattatta
    20521 cattaaaggt ggtcgcgtag attatggcgc agcacatgca gctaaatacg gacatgagcg
    20581 ctatggtaag acttataaag gaatcatgcc taattgggaa cctggtaaaa aggtacatct
    20641 tgtagggcat agtatgggtg gtcaaacaat tcgtttaatg gaagagtttt taagaaatgg
    20701 taacaaagaa gaaattgcct atcataaagc gcatggtgga gaaatatcac cattattcac
    20761 tggtggtcat aacaatatgg ttgcatcaat cacaacatta gcaacaccac ataatggttc
    20821 acaagcagct gataagtttg gaaatacaga agctgttaga aaaatcatgt tcgctttaaa
    20881 tcgatttatg ggtaacaagt attcgaatat cgatttagga ttaacgcaat ggggctttaa
    20941 acaattacca aatgagagtt acattgacta tataaaacgc gttagtaaaa gcaaaatttg
    21001 gacatcagac gacaatgctg cctatgattt aacgttagat ggctctgcaa aattgaacaa
    21061 catgacaagt atgaatccta atattacgta tacgacttat acaggtgtat catctcatac
    21121 tggtccatta ggttatgaaa atcctgattt aggtacattt ttcttaatgg ctacaacgag
    21181 tagaattatt ggtcatgatg caagagaaga atggcgtaaa aatgatggtg tcgtaccagt
    21241 gatttcgtca ttacatccgt ccaatcaacc atttgttaat gttacgaatg atgaacctgc
    21301 cacacgcaga ggtatctggc aagttaaacc aatcatacaa ggatgggatc atgtcgattt
    21361 tatcggtgtg gacttcctgg atttcaaacg taaaggtgca gaacttgcca acttctatac
    21421 aggtattata aatgacttgt tgcgtgttga agcgactgaa agtaaaggaa cacaattgaa
    21481 agcaagttaa attcatcttc tgaatttaat atgctatgta aatcgtgctg ttatcacggc
    21541 acatcagata taagtagcat cacagtgttg aatttaaaaa tagtaaagtg aaataaagcg
    21601 cctgtctcat tagcgaaaac taaagggaca ggcgtatctg tttatgagct taataaattg
    21661 tatgaataat atggttgatc gaataactgt ttatcatgat gataaattga gttttttaaa
    21721 ataatgatat attacatcat tgttatagcg tttaagaaat caacaacttt acgataaata
    21781 gtgattgctt cgtcattagg tctacgatca aaatcatgct cgtttttatt cacgcgttca
    21841 aatgttgaat gtggaacatg attcatgata tgttcgcttt cctcaacggg aacatcataa
    21901 tcgccattac aatgcgcaat gaaaacaggt ggaagtgttt taagttcatc tggtgcaata
    21961 ttatattttg aattagtata atcagcaatg ttaatcatat ttatccattt acctgtgcca
    22021 cgtgcataaa cgtagattaa aaaacgttgt gcgatttgat cttgaacaac cggtgttggt
    22081 gaagtgagtt gtgcaatcat tgtttcgttt acgctttgag ctatttttgc gtaataacta
    22141 ttagttgttt taaaaggttc agtgttgatg cgactataac cataaaaatc aataacacca
    22201 tcaatatctc tgtctcgtgc aattaataga cttaaatatg cacctgatga tctgccaaag
    22261 gtaaaaatag ggcaattaga atattgtgat tgaatcgcat cgaatgatgc gtagacatcc
    22321 tcaataatgc aatcgagact tacttctggt aataaacgat aacttagttg aattaaatcg
    22381 taatgttccg taaggatatc gatatactgt ggggataaat cgttagcttt accgaacatt
    22441 aatccaccac cgtggatgta gacaatagcg ccttttgttg gttgattttt tgctttaata
    22501 attgtgtaag gtaatgcaaa tgcatcttta gtaattactt tatctttaat ttcagtcacg
    22561 atttaatagg ctccttattt ttgatattga tgtcattata acactgtctt aaatttccat
    22621 gaaaaatagt cttaagacga tgagtcatga taattctgtt ccaattgacg taaagcgtca
    22681 cgggtatgct tctttagacc ttccccataa tccatcattt taacaatatc tttaaaagca
    22741 gcatgtggaa tggctaaatc ttctaaatct gccatagaaa attcaagatt gatatcatgt
    22801 ggtcgctgtt cagcaagttt atgcacaaag tcaggttctg tgacaaaagg cgaagacatg
    22861 ccgaccatat ctgcatgttg taaagcatct aaagcagact ctggagaatt aatcccgcca
    22921 cttgcaatta aagggatacg acctgctaaa tgttcataga caatttggtt aactggtcga
    22981 ccgaaatgat cacctggtgt acgagacgta ttttgataaa tatgtcgacc ccagctagcg
    23041 attgctaagt attggatgtt tgaaacgtcc atgacccaat tgattaattg gttgaactcg
    23101 tcaatggtat atcctaaatc actgcctctg gtttcttctg gcgttgctcg aaatcctaaa
    23161 ataaaattgt caggtgcttc tttatcaatc acttcttgta ccgcacgcat aacttctaaa
    23221 cataatcttg cacgattttt taatgagtcg gcaccgtaat ggtctgtacg tttattcgaa
    23281 aaagttgaga aaaatgtttg aatcagcaaa cgttgtgcaa tcgaaatttc cacaccatca
    23341 aaacctgctt taatcgcgcg taatgtagca tcgcgatact gctgaatgat gctattgatt
    23401 ttctcatgag acatggcgat aacatcgtgt tcaatcggtg aatgcaatgt catagggctt
    23461 ggtccataca cctttccaaa atttaaaatg gcttgatttg aaaaacgacc agcatgcgct
    23521 agctggataa tagcgaggct accatgttgt ttcatcgtag atgccatgtt agttaatcca
    23581 gggatacaag catcatgatc aatattaaag ccatattcaa acaattgacc ataaggttca
    23641 atgtaagcag cgccggtgac ttgcattcca gctgaattag agcgacgtgc agcataagcc
    23701 aagtcttctt ttgtaatata gccttctttt gttgatgtgt ttacggtcat tggtgataat
    23761 acaaagcgat tcgaaatttt gatgccatta ggtaagtgga ttgattgtaa aagtggtttg
    23821 tatcggtaca tactatgatt ccttttctat tcaatattgt tttcaaagta ccatggaaag
    23881 aatgaataat caatgatgaa cagtcttgat agaatagaat tggtacatgg aaagtatttt
    23941 taaaattaaa ctaatgaatg gcatttgtag gtctgaaaat atgaatatga aaaagaaaaa
    24001 taaaggcgaa aagatataaa agttaattga aaaacgttat catatacgtg ggtatatgaa
    24061 gagggaatgg tattaagaac gctaaaatgt tatgtcggtt tgacatgaca ggataagttt
    24121 ggagatgacg gattggttaa attaagcgta ttagactatg ccttaataga tgaaggtaag
    24181 gatgcacaaa aggcattgca agattcagtg acacttgcaa aattagcaga tcgacttggc
    24241 tttaagcgaa tttggtttac ggaacatcat aatgtaccag cgtttgcgtg tagtagtcca
    24301 gaacttttga tgatgcatac attggcgcag acaaatcaca tacgagttgg ctctggtggt
    24361 gtgatgctgc cgcactatcg accttataaa attgctgagc attttagaat gatggcagcg
    24421 ttatatccaa atcgtattga tttaggtatt ggtaataatc caggtactac tatggtaaag
    24481 caagctttag atggaataaa tcctacatat gatagttacg atgaatcgat ttcgttatta
    24541 cgtgattatc ttacaataaa ggataaacca agtgcgcata cgttaggtgt ccaaccacac
    24601 attgatcatt ttccagaaat gtggttatta agtagtagcg caacatctgc caaaatagct
    24661 gccgaactag gtatagggct ttctgttgga acatttttgc taccagatat aaatgcgata
    24721 catgcagcga aggataacat tgatatttac aaaaaacatt tccaagcatc aacgattaaa
    24781 atggacgcaa aggtgatggc atctgtattt gtcattgtag ctgataacga agcggaagta
    24841 gcagcattac aacatgcctt agatgtttgg ttattaggta aattacaatt tgcagaattt
    24901 gaagattttc cttcagtaga cacagcacaa aagtataagc ttaatgatcg agacaaagag
    24961 atgattcaag cacatcaagc acgcatcatt gcaggtacac aagaaaaggt taaagcacaa
    25021 ttagatgatt tcattgctac gtttgaagtt gatgaggtgt tagtagcacc gcttattcca
    25081 ggtattgaac agcgttgtaa aacattaaaa ttactcgcgg aaatttattt gtagcatttt
    25141 aaatagaaga gaaaggatga agataagatg aaaaagttag ccaattattt atgggtagaa
    25201 aaagtaggag atttgtatgt gtttagtatg acacctgaat tgcaagatga tattgggaca
    25261 gtaggttatg ttgaattcgt aagtccagat gaagttaaag tggatgatga aattgtgagt
    25321 atcgaagcat cgaaaacggt cattgatgtg caaacgccat tgtcaggaac gattattgag
    25381 cgaaatacaa aagcggaaga agaaccgaca attttaaact ctgaaaaacc agaagaaaat
    25441 tggttgttca aattggatga tgtcgataaa gaagcattcc tagcattacc ggaggcttaa
    25501 atggaaacgt taaaatcaaa taaagcgaga cttgaatatt taatcaatga tatgcatcga
    25561 gagagaaatg acaatgacgt attggtaatg ccatcttcat ttgaagattt gtgggaatta
    25621 tatcgaggct tagcaaatgt cagaccggca ttacctgtaa gtgatgaata tttagctgta
    25681 caagatgcta tgttaagtga tttgaatcgt caacatgtta cggatttgaa ggatttgaag
    25741 ccgataaaag gtgacaatat ctttgtttgg caaggtgata tcacgacgtt aaaaatcgat
    25801 gctattgtta atgctgcaaa tagtcgtttt ctaggatgta tgcaagctaa tcatgactgc
    25861 attgataata ttattcatac aaaagcgggt gttcaagttc gacttgattg tgcagagatc
    25921 attcgacaac aagggcgcaa tgaaggtgta ggtaaagcca aaataacacg tggatataat
    25981 ttgtcagcaa agtatataat tcatacggtt ggtccgcaaa tacgtcgatt gcctgtttca
    26041 aagatgaatc aggacttgtt agctaaatgt tatcttagct gtcttaaatt ggctgatcaa
    26101 catagtttaa atcatgtcgc tttttgctgt atatctacag gtgtatttgc ttttcctcaa
    26161 gatgaagcag cagaaattgc tgttcgaaca gtagaaagct atctcaaaga aacaaattca
    26221 acattgaaag tcgtgttcaa tgtatttaca gataaggatt tacaactgta taaggaggca
    26281 tttaaccgtg atgcagagta gtaagtggaa tgcaatgtct ctgttaatgg atgacaagac
    26341 aaagcaggct gaagtattgc gtactgcgat tgatgaagca gatgcgatag tgattggaat
    26401 tggtgcaggc atgtctgcat ctgacggatt tacatatgta ggagagcgtt ttacggaaaa
    26461 tttcccagat tttattgaaa aatatcgctt ctttgatatg ttgcaagcga gtttacatcc
    26521 ttatggcagt tggcaagagt attgggcatt tgagagtcgt tttattacat taaactattt
    26581 agatcaacct gtaggtcagt cttacctcgc tttaaaatcc ttggtggaag gtaaacagta
    26641 ccacattata actacgaatg cagataatgc tttcgatgta gctgattatg atatgactca
    26701 tgtatttcat atacaagggg agtatatact gcaacagtgt agtcagcatt gtcatgctca
    26761 aacgtatcgc aatgatgatt taattcgtaa aatggttgtt gcgcaacaag atatgcttat
    26821 accttgggag atgattccaa gatgtccaaa atgtgatgcc ccaatggaag tgaataaacg
    26881 taaagcggaa gttgggatgg ttgaagatgc tgaatttcat gcgcaactac atcgttataa
    26941 tgcttttcta gagcaacatc aagatgataa agtgttgtat ttggaaattg gaattggtta
    27001 tactacacca caatttgtga agcatccttt tcagcgtatg acacgtaaaa atgaaaatgc
    27061 cctttatatg acgatgaata aaaaggcata tcgcattccg aattcaattc aagaacgtac
    27121 catacattta actgaggata tctcaacatt gattacagca gcactccgga acgacagcac
    27181 aacgaaaaat aacaacattg gagagacaga agatgtactt aatagaaccg attagaaatg
    27241 gagaatatat tactgatggt gcgattgcac tcgctatgca agtttatgtt aaccagcata
    27301 tctttttaga tgaagatatt ttattccctt attattgtga tccaaaagtg gaaattggac
    27361 gttttcaaaa tactgctata gaagtgaatc aagattatat agataaacac agtattcaag
    27421 tagttcgccg agatactggt ggtggcgctg tgtatgttga taaaggtgcc gttaatatgt
    27481 gttgtatttt agaacaagac acttcaattt atggtgattt tcaacgattt tatcaaccag
    27541 ctataaaggc gttgcataca ttaggtgcaa cagatgtggt acaaagcggt agaaatgatt
    27601 taacattgaa tggtaaaaaa gtgtcaggcg ccgcaatgac attaatgaat aatcgtattt
    27661 atggcggtta ttcgctatta cttgatgtta attatgaagc aatggataaa gtgttaaagc
    27721 ctaatcgcaa aaagattgca tcgaaaggga ttaaatctgt gcgcgcacgt gttggtcatc
    27781 ttagagaagc actggatgaa aagtatcgtg atataaccat tgaagaattt aaaaatttaa
    27841 tggtgacgca gattttggga atcgatgaca ttaaagaggc gaaacgatat gaattaacgg
    27901 atgcagattg ggaagcgatt gatgaattag ctgataaaaa gtataaaaat tgggattgga
    27961 attatggcaa gtcacccaaa tatgaataca atcgaagtga aagattatct tcaggtacgg
    28021 tagacataac aatttctgtt gaacaaaatc gtatcgcaga ttgtcgtatt tatggggatt
    28081 tctttggaca aggtgatata aaagatgtgg aagaagcatt acaaggaaca aaaatgacaa
    28141 gagaagattt aacgcatcag ttaaagcaat tagacatcgt ttattatttt agcaatgtta
    28201 cggtagaagc attagtggat atgattttaa gttaatattg ttattttatg tatgctgaat
    28261 cattggaagt gtttgcttgc tcttgaaaag gtgacaatag tgtttggtga aggttgaaca
    28321 tatgagtgga aattattgcc tttaactatt caaagtatga tatatatatg gtttttgttt
    28381 ctaaatgatt gggtatttga aaatagatga gtttaatatt ttaaggaata taatgatgtt
    28441 tacttttata attcatatag aatattaagc aatataagtc tgttgatata tacaaaatat
    28501 aatgactgct ataatgagta atcaatagac acaaagagga gattatgtga tgaataataa
    28561 agtattagca accggtggta cagggtttgt tggcatgcga attatttcac gattattaga
    28621 acaaggttat gacgtacaaa cgacgatacg tgatttaagt aaagctgata aagtaattaa
    28681 aacaatgcaa gacaatggca tttccacaga gcgattaatg tttgtcgaag cggatttatc
    28741 acaagatgaa cattgggatg aagcaatgaa agattgcaag tatgtcttga gtgtagcatc
    28801 tccggtgttt ttcggtaaaa cagacgatgc agaagtgatg gcgaagcctg caattgaagg
    28861 tatacaacgt attttaagag ctgcagaaca tgcgggtgtt aaacgtgtgg taatgactgc
    28921 aaactttggt gcagttggtt ttagtaataa agataaaaat tcaatcacaa atgaaagtca
    28981 ttggacaaat gaagatgaac caggcttatc agtatatgaa aaatcaaaat tgttagctga
    29041 aaaggcagcg tgggattttg ttgagaatga aaatacaaca gtagaatttg ccacaatcaa
    29101 tccagttgca atttttgggc catcattaga tgcacacgtt tcaggaagct ttcatttatt
    29161 agaaaattta ttgaatggtt caatgaaacg tgtaccgcaa attccgttaa atgttgttga
    29221 tgtgagagac gtagctgaac tgcacatttt ggcaatgaca aatgaacaag ctaatggcaa
    29281 gcgatttatt gcgacggctg atggacaaat taatttgttg gaaattgcaa aattaattaa
    29341 agaaaagaga cctgaaatag ctcaaaaagt ttctactaaa aaattaccag actttatttt
    29401 gagtctaggt gctaaattta atcatcaagc taaagaaggt aaacttttat tagatatgaa
    29461 ccgcaatgta agtaacgaac gtgcaaaaac attacttggt tgggaaccga ttgcgacaca
    29521 agaagaagca attttagcag ctgtcgatag tatggctaag tatcatttaa tataatacaa
    29581 aacaccgtcc ataagtaagt ggctttcctc ttgataaaag gggcattcac tcaatatgtg
    29641 acggtgtttt atatttctac gttaacacta ttgctgttct ttttgacggc cttttaataa
    29701 aattgttgtc gcacctacga taataataaa tagaatcgca ccaaataatc ccatatattt
    29761 tactgcgtta ccgaacacga taccgacagc taaaaagtct gtatctgaga atgttgttgc
    29821 agcaccacct aattcgccta aaaatggcaa gaataataat ggtaaaaacg tgattaggat
    29881 accatttaga gcggcgccag caatagcacc tttaataccg cctcttgcat taccgaatac
    29941 agcagccgtt gcacctaaga agaagtgtgc aactacgcca ggtaaaatga cgacgccacc
    30001 aaataagaat aagataaaca taccgatgac acctgtaata aagctgacaa agaatccaat
    30061 taatactgca ttttgtgcat aagggaacac aatagggcag tctaatgcag gtttagaatt
    30121 tggtacaagc ttttcagaaa ttcctttaaa tgctgggacg atttcagcta agattaaacg
    30181 aacgcccgtt aaaataataa atacaccagc agcaaatgtc acaccttgaa ttaatgaaaa
    30241 gacaataaag ttttgaccat cactaataga ttcgtgtaca taactaacgc ccgcaaataa
    30301 gcatgcgatg aagtaaagta atgccatcgt aatcgagata ctaattgtac tttctcgtaa
    30361 gaaacttaag ccttttggaa atttaatctc ttccgttgat ttagacttac ctttgaataa
    30421 ttgacctaca gcacctgcgg caaagtaact gattgagcca aaatgaccta aagctacttg
    30481 gtcattccct gtaatttttc gcatcgtagg ttggagtaat gcaggtaata ctgccatgat
    30541 taatcctaat acgagtgcgc cgataacaat cgttagccag cctttaatat gactgactgt
    30601 taaaatgatt gctaaaaacg cagccatgta aaatgtatga tgacctgtta aaaagatata
    30661 ttttaaatta gtgaagcggg caattaaaat attaacaatc atgccacaga ccatgatgag
    30721 tgcagctgtt gttccaaaat cttttaaggc tagtgagacg atagcttcgt tgttaggtac
    30781 gataccttgc acaccaaatg cgtgttggaa tattttgccg aatggttcaa gagatcgaac
    30841 gacgacatca gcacctgcac ttaaaattaa gaagcctaat atcgttttaa tggttcctga
    30901 agtgatcgtt gcggcaggtt ttttctgaac gattaaacct ataaaggcaa tcagtgcaac
    30961 aagaatggct ggttgactta aaatatcgac tataaaatta aggattgctt gcataggtcg
    31021 tacctccttt aaatcatgtt aagttgttgt aatttttctg agagcttttg ttgtaattca
    31081 gctttatcta aaatattatc aagaactaag acatccccta gacgttcggc attttcagct
    31141 aaatctctac cacaaataaa caagtcagcc atctctggac ttgctgtcat aatgtcacta
    31201 tgttcaactt cgatatcaga tggtgcatta agttgcctaa gtgcttcttg tgcgttcatt
    31261 tctaccataa aactacttcc taaaccgtgg ccacatacta ctaaaatttt catattaatc
    31321 atgctccttt aaaatgtttt taatgtcttg tgcatttgtt gcagttaata gttgctggac
    31381 tgtttggtta tcgcccagta cggttgctaa attttgtaat acagataagt gtgaatgatt
    31441 gtcgatggca ctcaatacaa aaatgagaga tgcgtagtga tcttcatcac aaaatgccac
    31501 atgttgattc aactttaata gacttaaacc aacttgatgt acgtcattgt tcggtcttgc
    31561 atgtgcaatt gcaatttcag gtgcgataac gatataaggt ccaagttcat taacgctatc
    31621 aatcattgct tgaacatagc cttgttcaat aatttgttct tgtagtaatg gctgagaagc
    31681 tatagttata gcttcagtcc aatcatttac ttgttctttt acaatgatgc gtgttgttga
    31741 caaaatgtct aatgacacgt tattaagcct cctttgtcat agttaaagca atgtgttgtt
    31801 taattttaaa aatattccca tctaagaaat cttgtcgata taagtcgttg cttaagcatt
    31861 cgcttaactg tcccaatgcc tttaaatgtg cattggggtg gtccgttgct aatgtaatta
    31921 caaggtgaac gggatcgtta gctttactac caaagataat cccttcagtg aaatatgtta
    31981 gtgcgaaacc tacaccattc tgtacataat cagtaccagc gtgaataagt gcaatatgtg
    32041 gactaatgac catatatgac ccgaattgtt caaattgttt taaaattgca gctgtataat
    32101 ttgaatagac aatgccatca ttgattaaag gttgcacagc cactgcaatt gcggattcaa
    32161 ttgataatgg ttgtttattt ataatgatgc gatgttcagg caataaatct gcgagtgact
    32221 tgccatcagt tgccattttc atgactcgtt gttctcttga gtcattgata atttgattca
    32281 atttttgacg agattgttga ttgataaatg gatcgacatg aataactggt acagctgata
    32341 tttcacaagg tactgttgaa atgacataat caatgttatc ttgcaataat cgactttctt
    32401 ccaattgata aatggaatag gcatcccaaa tgtgaaactc aggatacagg tgatttagtt
    32461 ttgattttaa aagttgtgac gtgcctatac cagaaccaca tagtaagaca accttaatca
    32521 ttgattgttt atgtgttgca acacgctcta tacttgatgc gaagtgaatt gtaatgtatg
    32581 ttaattcatc ttcgttgaag cgaatagcag catcttgttc aattggacta atatgcttgc
    32641 taacggcttc aatgatttga ggatagcgac gcataacttc ttgcctcaaa ggattaggtt
    32701 gtagcatatc gtatttaata cgatgtatag ctggtttgat atgtgtgatc agactggtat
    32761 gtaacttgtt gtcttttgac atatcaatgc ctaattcttg gctaacacaa gtgatcaatt
    32821 catgtatatt ttgcgataaa tcatggtatt caaaggtaat tgaagatgct gtatgttcag
    32881 tcattttaga gcctagtaaa tgtaacgtga taaagataat ttcagactct ggaaatgtga
    32941 cattacaact gcgttctaag ttttctatca tttttgaagc aatagcatac tgattagtat
    33001 gtcgccattt atcaatttca ttgataggta tatcgaacga aaaattttca tttaaacgct
    33061 gaatggcaat gagtatatga tagattaagc catcgatagc cgactgaact aaatgataat
    33121 tttcactatt taatgtctta ataatggcac ggcgaaccaa tgcgattgat tctgaattaa
    33181 agatatccgc ctctataaaa ggtgcagctt gtttcatata ttgatgtata aagtgtgcat
    33241 acgctttacg ataatgatct tcctcaccaa taatattgaa tcctttattg tggacataat
    33301 ttaactttaa atggtattga tctagttggg cttgaatcat tttaatatca tctgcaattg
    33361 tccgacgcga aacattaaca tcttgcgcaa gttgctttgt tgaaacagga tcggttgttt
    33421 cgaataactt taaagcgata tgtgtgagtc gttcatcttt tgaaaaatga atttgatttg
    33481 ttagattgtg ctctaattca tttaatagtg tcgcgtgagc tgttgttact ttgatgcctg
    33541 cagctttatt acggctgact tggtaatgat aagtttcagc atattgctca atatatgcta
    33601 tatcatattg aatggtacga ggtgatacac caagttgatt agcaatggta ttgattggaa
    33661 taaacgtttg ctcatgaatt aaaagataca aaatttcgat ttgtctataa cttaacaacg
    33721 taatatcctc ctatttgtaa ttgtaagcga tttcttaaaa acgtagatat gcaatctctt
    33781 tcatatttta atccgaaaaa ttgcatatca aaatgtttat ggcgcaagat tttataggaa
    33841 cttttaaaat aaattagata ttcatgttgt caatttaaaa atgtcgcagt atatttagtt
    33901 agacatctaa cgaaatggtg gtgcaataaa tggaattcac ttattcgtat ttatttagaa
    33961 tgattagtca tgagatgaaa caaaaggctg atcaaaagtt agagcaattt gatattacaa
    34021 atgagcaagg tcatacgtta ggttatcttt atgcacatca acaagatgga ctgacacaaa
    34081 atgatattgc taaagcatta caacgaacag gtccaactgt cagtaattta ttaaggaacc
    34141 ttgaacgtaa aaagctgatc tatcgctatg tcgatgcaca agatacgaga agaaagaata
    34201 tagggctgac tacctctggg attaaactcg tagaagcatt cacttcgata tttgatgaaa
    34261 tggaacaaac actcgtatcg cagttatctg aagaagaaaa tgaacaaatg aaagcaaact
    34321 taactaaaat gttatctagt ttacaataaa tgataagtgt gactggtaga aatcagtcac
    34381 tttgtcttta atattatagt tagatatcta attgttagta agctaattat tggaaaagac
    34441 aaggagtatt gaacaatgaa agacgaacaa ttatattatt ttgagaaatc gccagtattt
    34501 aaagcgatga tgcatttctc attgccaatg atgataggga ctttattaag cgttatttat
    34561 ggcatattaa atatttactt tataggattt ttagaagata gccacatgat ttctgctatc
    34621 tctctaacac tgccagtatt tgctatctta atggggttag gtaatttatt tggcgttggt
    34681 gcaggaactt atatttcacg tttattaggt gcgaaagact atagtaagag taaatttgta
    34741 agtagtttct ctatttatgg tggtattgca ctaggactta tcgtgatttt agttacttta
    34801 ccattcagtg atcaaatcgc agcaatttta ggggcgagag gtgaaacgtt agctttaaca
    34861 agtaattatt tgaaagtaat gtttttaagt gcaccttttg taattttgtt cttcatatta
    34921 gaacaatttg cacgtgcaat tggggcacca atggtttcta tgattggtat gttagctagt
    34981 gtaggcttaa atattatttt agatccaatt ttaatttttg gttttgattt aaacgttgtt
    35041 ggtgcagctt tgggtactgc aatcagtaat gttgctgctg ctctgttctt tatcatttat
    35101 tttatgaaaa atagtgacgt tgtgtcagtt aatattaaac ttgcgaaacc taataaagaa
    35161 atgctttctg aaatctttaa aatcggtatt cctgcatttt taatgagtat cttaatggga
    35221 ttcacaggat tagttttaaa tttattttta gcacattatg gaaacttcgc gattgcaagt
    35281 tatggtatct catttagact tgtgcaattt ccagaactta ttatcatggg attatgtgaa
    35341 ggtgttgtac cactaattgc atataacttt atggcaaata aaggccgtat gaaagacgtt
    35401 atcaaagcag ttatcatgtc tatcggcgtt atctttgttg tatgtatgag tgctgtattt
    35461 acaattggac atcatatggt cggactattt actactgatc aagccattgt tgagatggcg
    35521 acatttattt tgaaagtaac aatggcatca ttattattaa atggtatagg tttcttgttt
    35581 actggtatgc ttcaagcgac tgggcaaggt cgtggtgcta caattatggc cattttacaa
    35641 ggtgcaatta tcattccagt attatttatt atgaatgctt tgtttggact aacaggtgtc
    35701 atttggtcat tattaattgc tgagtcactt tgtgctttag cagcaatgtt aatcgtctat
    35761 ttattacgtg atcgtttgac agttgataca tctgaattaa tagaaggtta aatatttcgt
    35821 ccacttctgg ctgagtatat ttcggtcgga agtgtatttt tcgaaaaaaa taaatatatg
    35881 atacgattat gaaaaaataa agtgagattg gcatatgtgt aaatctaaaa tactgttgaa
    35941 aaatattttt agtgaagaat cagaagttaa agatttaact gaagaaaaat ataatcaaga
    36001 ttacgaagca ttaacattta gctttaaaga ggaaacatat caaagtaggt tagctaagaa
    36061 aacaccgact aaatcgggat atttcgtgac atgttggaca aaagacgaag ataattataa
    36121 tcggccatac aaaattgaag agtttgctga ttacctgatt gttgctgtta tcgatgatga
    36181 attaaatggc tactttctat ttcctaggga attattggta gaaaaaggta tcttagcttc
    36241 atctaagtat caagggaaaa tggcttttag agtttatcct aagtggtgta atcaattgaa
    36301 taaaacagca gggcaaacac aaaagtggca atgtaaatat ttttttgaat actaataaaa
    36361 agtcatatgg ttgaacttat aagagatgaa tatatcatct ttcgataaga taaccatatg
    36421 actttatttt ttatcattta atgatgaacg gtttcttgtc ctactttatt ccaagtgagg
    36481 ataaagctca acattgcaaa cacactaatt gctgttaata aaataaaacc gacatcccat
    36541 ccgaatttat caactacagc acctaagacg atgttggcca ttacagcacc aaacagataa
    36601 ccaaataatc ctgttaatcc agctgctgtg ccagctgctt ttttaggtac ataatctaat
    36661 gcttgtaaac caattaacat aactggtcca tatattaaga aaccaatggc aattaatgag
    36721 acattgtcta accaagcatt gcctggagga tttaaccaat aaattaatac aaatactgtg
    36781 acacctaaca taaagaagaa acctgcaggt ccacgacgac ctttgaataa tttatcagaa
    36841 atgtaaccac ataataatgt accaggaatt ccagcccatt cgtataagaa gtatgcccaa
    36901 cctgatgctt ttaagtcgaa atgtttttct tcacttaagt agactggcgc ccaatcaagt
    36961 acaccataac gcacgaaata aacaaatata tttgcaaagg caattgccca tacccattta
    37021 ttgttcagta catatttaaa taaaatttct tttgtagtta attctgtttc taatgttttc
    37081 ttatcgcttg tagcaaagtc atttttataa atttcgattg gaggtaaacc ttgagattga
    37141 ggtgtgtctc taatcaatac gtatgaaatt gcggcaatga taagtgctaa gagtgcaggg
    37201 taaatgaata caccttcgaa accttttaaa taaccaaagt tgataaatgc agttgttgta
    37261 ataccccaag cagcaatagg tgccataata cctccaccaa cattatgcgc aacgttccaa
    37321 agggcagtct tacttccgcg ttcacttaca ctaaaccagt gaacgagaac acggcctgaa
    37381 ggtggccagc ccataccttg aaaccatcca tttaagaata ataggacaaa cataataccg
    37441 atacctgatg tgaagaacgg tacaaatccc attaacaaat tgacgatagc agtgagtgct
    37501 aatccaagaa ctaagaatat ccgagcattg ctccgatcac ttacagtacc cataaagaac
    37561 ttactaaatc catatgcgat ggaaacagca gaaagtgcaa aacctagttc cgcttttgta
    37621 aaaccttgct cttgcaatgc cggcatcgct aacgaaaagt ttttacgtaa taaatagtac
    37681 ccagcataac cgatgaaaat accaagaaat acttggagac gtaatcgttt ataggtatca
    37741 tctatctgat tttctggcaa aggcttaata tgctttgcag gtttaagaaa attcataaaa
    37801 tcctccttaa tatgtattta tatgcatttt gtgcgataaa cttatattag acatgtatga
    37861 aagcgttgtc aatatgcttt tgtgaaatct tgcatattta gttattgaag tattttataa
    37921 acatttagaa aaaataaatt gctacacata aatttataat tatgcaatta ttatgtcgtt
    37981 atacatacta gctgtgtatc tacgaatgag tacaaacata tttttatttg caaggggtaa
    38041 atggcatata actatctttt ttatgtaagc tggtataaaa tttatactaa taggagggat
    38101 agtatgaata tagtagggca tcatcacata tccatgtata caaaagatgc aaaacgtaat
    38161 aaggattttt acacaaatgt ccttggatta cgattagttg aaaagtcggt taatcaagac
    38221 aatccttcaa tgtatcattt gttttatggg gacgaagtag gtacagccgg aacaatttta
    38281 agcttttttg aaattcccaa tgcgggtcat aagcagccag gtactgaaac gatttatcga
    38341 ttttcattat tagtaccaaa tcaagcggca cttcattatt ttgaaaaacg tcttgagaat
    38401 aatggtatta agtctgaacg tttgtactat cttggacaag aaggtgttgt ctttaaagat
    38461 gaagacgact tagaaatcat attgcttgtt aatgatagtt ttgaagtacc acatcaatgg
    38521 caacataacg cttatagtga aatacctcaa gcatatcaaa ttttaggaat agggccagtc
    38581 gaattaagag ttagaaatgc agcgcgtacg gtagaatttt tggaaaatgt cttaggttat
    38641 cgcaaaagag ataataaatc attcgatgtg ctgacattag caccacaagg tttatattcg
    38701 gattttgtag ttattgagca acagggacaa cgtgaaagac ctggacgagg ttatatccat
    38761 catattgcag ttaatacacc acaaatgagt gacttagatg caatttacaa gaaattacaa
    38821 caacaaccac aaagtaattc aggtataatt gatcgctatt tctttaaatc attatactat
    38881 cgccataatt caattatgta tgaatttgcg actgaagcgc ctggatttac tattgataca
    38941 cctgttgaac aattaggaag tcaattgaac ttgcctgact ttttagaagc agaacgtgaa
    39001 caaattgaaa gtaagttaca cgaaatataa aggagaatgt ttaatggcca aattagaaat
    39061 gaataaaaat acgcctcttg agtttggttt gtattcctta ggtgatcatt tattgaatcc
    39121 attgaaaggt gaaaaagtta gttatgagca acgtattaat gaaattattg aagcaagtaa
    39181 attagcagat gaagcaggta ttgatgtttt tgcagttggt gaaagtcatc aggagcattt
    39241 tacaacacag gcacatacgg ttgtgttagg tgcaattgcc caagcgacaa agcatattaa
    39301 agtttcaagt tcttcaacga ttattagtgc aacagatcct gtaaaagtat ttgaagactt
    39361 cgcgacatta gatttgattt ctcatggtag agccgaaatt gtagctggca gagcatcaag
    39421 aacaggtatt tttgacttgt ttggctatga tttaaaagac tatgatgaat tgtttgaaga
    39481 aaaattaggt ttacttttag agttaaataa aactgagcgt attacttggt ctggaaaata
    39541 tcgtccagaa cttagaaata tgaaaatatt cccaagacca atcgataata tattgccaat
    39601 atggcgtgct gttggtggtc cacctgcaag tgctattaaa gcgggaaaac aaggtgtgcc
    39661 aatgatgatt acaacccttg gtggcccagc aatgaacttt aaaggttcta tagatgctta
    39721 tcgtcaagcg gcaactgaag caggtttcga tgcttcgcct aagtctttac cagtaagtac
    39781 agcgagtctg ttttatacag ctgaaacaac tcaggatgct atgagagaat tttatccaca
    39841 tttgaataca gggatgtcat ttattcgtgg tgttggttat ccgaaacagc aatttgctaa
    39901 ttcgtcagat tatcgagaag cgctaatggt tggaagcccg caacaaatta ttgaaaagat
    39961 attgtatcaa cacgagttgt atggtcatca acgttttatg gcacagcttg attttggcgg
    40021 tgtgccattt gaaaatgtta tgaagaatat tgagttaatt ggcaacgaca ttataccggc
    40081 gattaaaaag catttatcaa aataggaggg gcgtcatcat gaatattgta ttattgtcag
    40141 gttccacagt aggttctaaa acgagaattg ctatggatga tttaaaaaat gaactagaag
    40201 tcatcaatga gggacatcaa atagagttga tggatttacg agaacttgaa ttagaattta
    40261 gcgttggaaa gaattatcta gatactacag gagatgtata taaattaacg acgtcgttaa
    40321 tgcaggctga tgtgattttt attggttttc caatttttca agcttccatc cctggtgctt
    40381 tgaaaaatgt gtttgatcta cttccagtca atgcgtttcg tgacaaggta ataggacttg
    40441 tagcgacagc aggttctagt aaacattatt taattcctga aatgcattta aaaccaatat
    40501 tgagttacat gaaagcacat acgatgcaaa cgtatgtatt tattgaagag aaagattttt
    40561 caaatcaaca aattgtcaat gatgatgttg tatttcggtt aaaagcgttg gcacaatcca
    40621 caatgcgaac tgccaaagta caacaacaag tgtttgaaga agaaaacaac caatacgact
    40681 tttaaagtat aaaaataaga cgctcggcac actaaatttg taagtgtttg agcgtctttt
    40741 catattaact atatagccaa tgaacgacga taaaggcaag tgatgacaag catattgagg
    40801 taataatgat tgtcataagc ggtttaagtg cgcgattttt aagatcttta aatgcaacat
    40861 ttaaccctaa agcaaccatg gccattaata agcaaattgt tgatacagta tttaaaatat
    40921 ttagcaatgc tgacggaata gttacatatg tattcactaa ggccataatg acaaatccaa
    40981 ttaaaaagta tggaatgctt attcgaccct tgctagatga ttctgatgaa cggaaacgca
    41041 taattaaaat aagtacgatg gttaatggaa tcagtaagaa tactctacca agtttaccaa
    41101 gaagtgcaat tttaagtgca tcactaccac caaagccacc agctaagaca acgtgtgcaa
    41161 tttcatgaag actaacacca gaccaagcgc cataaacatt tgtcgtcatt gaaaagatag
    41221 cgtagatagc tgtatatata agtgaaaata tcgtaccaat caatgcgatg ataccgatac
    41281 taatagctgt atccttttca cgtgatttga atattggagc gactgcggca atagcagcag
    41341 caccacaaac gcctgtgccg acacctagta ataatgcgat gtttttgtca ccatgcaaca
    41401 gtttgttgac aaagagcatc attacaatac tgaaaataac gacacctaca tcgatggcta
    41461 atagtttact accttgaccg ataatatcga atatattgag tttaagtcca tataggatga
    41521 ttgcaaatct taataaatat ttagatgaaa acgtaatacc tgagctatat tgttcaggat
    41581 atcctctaaa gtgacgatat agaatagcga ttaatatcgc gatagttaat gcgccaacct
    41641 tatctaggat tggcaattta gctgctaaaa agctaaataa tgcgactata aatgttagtg
    41701 atagcccaat cataaaatgc ttatttttca atgatgccat gagcagtgcc tcctttaata
    41761 gcattttagc actgttttgt cgtattttta aatataaatt tggaatgaat aataaagtag
    41821 tgattaaatt aagttgtgtg ataggaaact tggacatcaa tcaaagtaat aggcactaca
    41881 acgcttattg gcggggcccc aacaaag
//
LOCUS       DALAAR010000018        38953 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000018 DALAAR010000000
VERSION     DALAAR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.018"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            58..273
                     /locus_tag="O2458_000937"
     CDS             58..273
                     /locus_tag="O2458_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886821.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            298..561
                     /locus_tag="O2458_000938"
     CDS             298..561
                     /locus_tag="O2458_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3886822.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            573..734
                     /locus_tag="O2458_000939"
                     /pseudo
     CDS             573..734
                     /locus_tag="O2458_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            827..1144
                     /locus_tag="O2458_000940"
     CDS             827..1144
                     /locus_tag="O2458_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886823.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            1137..1439
                     /locus_tag="O2458_000941"
     CDS             1137..1439
                     /locus_tag="O2458_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ3886824.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            1444..1704
                     /locus_tag="O2458_000942"
     CDS             1444..1704
                     /locus_tag="O2458_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ3886825.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            1718..2080
                     /locus_tag="O2458_000943"
     CDS             1718..2080
                     /locus_tag="O2458_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886826.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            2077..3243
                     /locus_tag="O2458_000944"
     CDS             2077..3243
                     /locus_tag="O2458_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCZ3886827.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            3269..3826
                     /locus_tag="O2458_000945"
     CDS             3269..3826
                     /locus_tag="O2458_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCZ3886828.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            3894..5846
                     /locus_tag="O2458_000946"
     CDS             3894..5846
                     /locus_tag="O2458_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCZ3886829.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5859..6044
                     /locus_tag="O2458_000947"
     CDS             5859..6044
                     /locus_tag="O2458_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCZ3886830.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            6044..6445
                     /locus_tag="O2458_000948"
     CDS             6044..6445
                     /locus_tag="O2458_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCZ3886831.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            6445..6702
                     /locus_tag="O2458_000949"
     CDS             6445..6702
                     /locus_tag="O2458_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCZ3886832.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            6705..6905
                     /locus_tag="O2458_000950"
     CDS             6705..6905
                     /locus_tag="O2458_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886833.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            6920..7162
                     /locus_tag="O2458_000951"
     CDS             6920..7162
                     /locus_tag="O2458_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886834.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7177..7425
                     /locus_tag="O2458_000952"
     CDS             7177..7425
                     /locus_tag="O2458_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ3886835.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            7418..7954
                     /locus_tag="O2458_000953"
     CDS             7418..7954
                     /locus_tag="O2458_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCZ3886836.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            7991..8197
                     /locus_tag="O2458_000954"
     CDS             7991..8197
                     /locus_tag="O2458_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ3886837.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            8194..8397
                     /locus_tag="O2458_000955"
     CDS             8194..8397
                     /locus_tag="O2458_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886838.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            8390..8626
                     /locus_tag="O2458_000956"
     CDS             8390..8626
                     /locus_tag="O2458_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886839.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            8619..9005
                     /locus_tag="O2458_000957"
     CDS             8619..9005
                     /locus_tag="O2458_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886840.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            9002..9154
                     /locus_tag="O2458_000958"
     CDS             9002..9154
                     /locus_tag="O2458_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3886841.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            9222..9422
                     /locus_tag="O2458_000959"
     CDS             9222..9422
                     /locus_tag="O2458_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ3886842.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            9710..12157
                     /locus_tag="O2458_000960"
     CDS             9710..12157
                     /locus_tag="O2458_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCZ3886843.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(12266..12364)
                     /locus_tag="O2458_000961"
     CDS             complement(12266..12364)
                     /locus_tag="O2458_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886844.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            12498..12788
                     /locus_tag="O2458_000962"
     CDS             12498..12788
                     /locus_tag="O2458_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCZ3886845.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            12769..14136
                     /locus_tag="O2458_000963"
     CDS             12769..14136
                     /locus_tag="O2458_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ3886846.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            14149..14586
                     /locus_tag="O2458_000964"
     CDS             14149..14586
                     /locus_tag="O2458_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3886847.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            14743..15057
                     /locus_tag="O2458_000965"
     CDS             14743..15057
                     /locus_tag="O2458_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ3886848.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            15184..15489
                     /locus_tag="O2458_000966"
     CDS             15184..15489
                     /locus_tag="O2458_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCZ3886849.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            15479..17170
                     /locus_tag="O2458_000967"
     CDS             15479..17170
                     /locus_tag="O2458_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ3886850.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            17175..18413
                     /locus_tag="O2458_000968"
     CDS             17175..18413
                     /locus_tag="O2458_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ3886851.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            18397..19170
                     /locus_tag="O2458_000969"
     CDS             18397..19170
                     /locus_tag="O2458_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ3886852.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            19182..20345
                     /locus_tag="O2458_000970"
     CDS             19182..20345
                     /locus_tag="O2458_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ3886853.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            20414..20692
                     /locus_tag="O2458_000971"
     CDS             20414..20692
                     /locus_tag="O2458_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCZ3886854.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            20704..21036
                     /locus_tag="O2458_000972"
     CDS             20704..21036
                     /locus_tag="O2458_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886855.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            21033..21434
                     /locus_tag="O2458_000973"
     CDS             21033..21434
                     /locus_tag="O2458_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886856.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            21435..21830
                     /locus_tag="O2458_000974"
     CDS             21435..21830
                     /locus_tag="O2458_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCZ3886857.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            21865..22506
                     /locus_tag="O2458_000975"
     CDS             21865..22506
                     /locus_tag="O2458_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ3886858.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            22598..23053
                     /locus_tag="O2458_000976"
     CDS             22598..23053
                     /locus_tag="O2458_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCZ3886859.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            23111..23461
                     /locus_tag="O2458_000977"
     CDS             23111..23461
                     /locus_tag="O2458_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886860.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            23503..23661
                     /locus_tag="O2458_000978"
     CDS             23503..23661
                     /locus_tag="O2458_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886861.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            23675..29875
                     /locus_tag="O2458_000979"
     CDS             23675..29875
                     /locus_tag="O2458_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCZ3886862.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            29875..30699
                     /locus_tag="O2458_000980"
     CDS             29875..30699
                     /locus_tag="O2458_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ3886863.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            30708..32291
                     /locus_tag="O2458_000981"
     CDS             30708..32291
                     /locus_tag="O2458_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ3886864.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            32291..32581
                     /locus_tag="O2458_000982"
     CDS             32291..32581
                     /locus_tag="O2458_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886865.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            32597..34507
                     /locus_tag="O2458_000983"
     CDS             32597..34507
                     /locus_tag="O2458_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886866.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            34507..35973
                     /locus_tag="O2458_000984"
     CDS             34507..35973
                     /locus_tag="O2458_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCZ3886867.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            35973..36362
                     /locus_tag="O2458_000985"
     CDS             35973..36362
                     /locus_tag="O2458_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCZ3886868.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            36355..36519
                     /locus_tag="O2458_000986"
     CDS             36355..36519
                     /locus_tag="O2458_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCZ3886869.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            36565..36864
                     /locus_tag="O2458_000987"
     CDS             36565..36864
                     /locus_tag="O2458_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ3886870.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            37000..37302
                     /locus_tag="O2458_000988"
     CDS             37000..37302
                     /locus_tag="O2458_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ3886871.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            37313..38767
                     /locus_tag="O2458_000989"
     CDS             37313..38767
                     /locus_tag="O2458_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ3886872.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT        14941 a         5283 c         7846 g        10883 t
ORIGIN      
        1 atacttatca cctccttagg ttgataacaa cattatacac gaaaggtgga acaacaaatg
       61 aacaaaaaat cagaagggtt agacataatc ataccaagga ttttcagaag agatcatgcg
      121 tcagtagaat ctttaacaga aaatgaacgt cgactaagaa aggaaatatt aggaagtatt
      181 aaaaaaggtg attacagcta cttagaaata aacaaagttt tctatgcatt agatagagaa
      241 cttcaataca gagcgaataa taacaaactt taacatttat cgaaaggagt gatagagatg
      301 ccaaaaatca tagtaccacc aacaccagaa aacacatata gaggcgaaga aaaatttgtg
      361 aaaaagttat acgcaacacc tacacaaatc catcaattgt ttggagtatg tagaagtaca
      421 gtatacaact ggttgaaata ttaccgcaaa gataatttag gtgtagaaaa tttatacatt
      481 gattattcac caacaggcac tctgattaat atttctaaat tggaagagta tttgatcaga
      541 aagcataaaa aatggtatta ggaggatatt aaatgagcga cacatataaa agctaattaa
      601 tagcagtact gtgcttcaca gtcttagcga ttgtgcttat gccattgctg tacttcacta
      661 cagcatggtc aattgcggga ttcgcaagta tcgcaacatt catatactac aaagaatact
      721 tttatgaaga ataaaaaaac tgctacttgc gtcaacaagt aacagtgaca aacatttatc
      781 aaaatataca acttaattaa atcaaaatat acggaggtag tcaactatgg ctgaaaatat
      841 taaaactgaa caacattatt acactaaaga tttctcagga tacagaaatg aagaagataa
      901 ctttgtagca aatcaagaat tgacagtaac aatcacattg aacgagtaca gaaaacttat
      961 tgaaataaag gctgttaaag ataaagaaga agatacttac agaggtaagt attttgcgga
     1021 agaaagaaaa aacgaaaaat tggaaaaaga aaatataaaa ctaaaaaaca aaatttatga
     1081 attacaaaac gaagaagata acgaggagga cgaagaaaac gaggaggaag tcaacaatga
     1141 ctaaaaaata taaagacatg actcaggaag aagtaaaaga cttattatct gaaaaaagcg
     1201 gagaattgta tgaattagcg aaagaaatta agggagaaag taaatttgat attttgcttt
     1261 tctcatcaat aggagttatc gacggagatt atttagcagg ttcaagttct gtgattggtc
     1321 atactttcga tcttgcttcc ttattggata gcactaagag ttataaagac attgtcaatg
     1381 ttctccaaat gtgtaaatca caaaaatttc acggtattga tgataacaag gagggctaaa
     1441 acaatgtatt acaaatttgg tgagataaaa aataaaatta tcagctttaa cgggtttgaa
     1501 tttaaagtgt ctgcgatgaa aaaacatgac ggtatcagta tacaaatcaa ggatatgaat
     1561 aatattccac ttaaatcatt tcatgttgta gatttaagcg aactatattt tgcgacggat
     1621 gcaatgcgtg acgttataaa cgaatggatt gaagagaaca cagatgaaca ggacagacta
     1681 attaacttag tcatgaaatg gtaggaggat ttaatcaatg gcaatattag aagatatttt
     1741 tgaagaatta aaactattaa ataacaattt acgtgtgtta aacactgaac tatcaacagt
     1801 agattcatca attgtacaag agaaagttaa agaagcacca atgccaaaag aagaaacagc
     1861 tcaactggaa tcaattgaag aagttaagga aacttctgct gatttaacta aagattatgt
     1921 tttatcagta ggaaaagagt tccttaaaaa agcagatact tctgataaga aagaatttag
     1981 aaataaactt aacgaacttg gtgcggataa gctatctact atcaaagaag agcattatga
     2041 aaaaattgtt gattttatga atgcgagaat aaatgcatga agctagatca ctcaaataga
     2101 gctcatgcaa agcttagtgc aagtggagca aaacaatggc taaactgccc accgagtatt
     2161 aaggcaagtg aaggtattgc agataaaagt acagtttttg ccgaagaagg tacattcgcc
     2221 catgagttaa gtgagttata tttcagcctt aaatatgaag gcctaacaca gtttgagttt
     2281 aataaggctt ttcaaaatta taagagaaat caatattaca gcgaagaatt gcgtgaatat
     2341 gtggaagagt atgtagctaa tgtagaagaa aaatataacg aagctttgag tagggataat
     2401 gatgtaatag ctttatttga aacaaaatta gatttaggta aatacgtccc tgaatctttt
     2461 ggaactggtg atgtcattat attttcaggt ggtgtacttg aaattattga ccttaaatat
     2521 ggtaaaggca ttgaagtttc agctatagat aatcctcaac ttagattata tggcttgggc
     2581 gcatatgaac tgcttagttt aatgtatgac attcatacaa ttcgcatgac tatcatacaa
     2641 cctcgaatag ataacttttc tactgaagag ttaccaatat caagattact tcaatgggga
     2701 gccgattttg ttaaaccatt agccagactt gcttataacg gtggaggaga gtttaaagca
     2761 ggtagtcatt gtagattctg taagataaat cattcatgta gaacacgtgc agaatacatg
     2821 caaaatgtgc ctcaaaagcc accacatttg ttaagtgatg aagagattgc agaactttta
     2881 tataaactgc ctgatatcaa aaaatgggct gatgaagtag aacaatatgc actaaatcaa
     2941 gcgaaagaaa atgataaaaa ctatcctggt tggaagcttg tagaaggtcg ttcacgaaga
     3001 atgataactg atacaaaagc aatgcttgaa aagttagttg aagcgggtta taaacctgaa
     3061 gatattacag aaaccaagtt acttagcatt acgaatttag aaaaattaat tggtaaaaaa
     3121 gcattttcta aaattacaga gggttttata gaaaagccgc aaggtaaatt aacacttgct
     3181 accgagtcgg ataaacgacc agctataaag caatctgctg aagatgattt tgacaaacta
     3241 taaaaattta aaaggacggt atataaacat gaaagcaaaa gtattaaata aaactaaagt
     3301 gattacagga aaagtaagag catcatatgc acatattttt gaacctcaca gtatgcaaga
     3361 agggcaagaa tcaaagtatt caatcagttt aatcattcct aaatcagata caagtacgat
     3421 aaaagccatt gaacaagcta tagaagctgc taaagaagaa ggaaaagtta gtaagtttgg
     3481 aggcaaagtt cctgcaaatc tgaaacttcc attacgtgat ggagatactg aaagagaaga
     3541 tgatgtcaat tatcaagacg cttattttat taacgcatca agcaaacaag cacctggtat
     3601 tattgaccaa aacaaaatta gattaacgga ttctggaact gttgtaagtg gtgattatat
     3661 tagagcttca attaatctat ttcctttcaa cacaaatggt aataagggta tcgcagttgg
     3721 attgaacaac attcaacttg tagaaaaagg cgaacctctt ggcggtgcaa gtgcagcaga
     3781 agatgatttc gatgaattag acactgatga tgaggatttc ttataagtca ataggtgggg
     3841 ttttagcccc actttaattt taaagaaatt gaggtgtcaa gaatttgaga tttatgaata
     3901 tagatattga aacatatagc agtaatgata tttcgaaatg tggtgcctat aaatacacag
     3961 aagctgaaga tttcgaaatt ttaattatag cttattcaat agatggtgga gcgattagtg
     4021 cgattgacat gactaaagta gataatgagc ctttccacgc tgattttgag acgtttaaaa
     4081 ttgctctttt tgatcctgct gtaaaaaagt atgcattcaa tgctaatttc gaaagaactt
     4141 gtcttgctaa acattttaat aaacagatgc cacctgaaga atggatttgc acaatggtta
     4201 attcaatgcg tattggctta cctgcttcgc ttgataaagt tggagaagtt ttaagactac
     4261 aaaaccaaaa agataaagca ggtaaaaatt taattcgtta tttctctata ccttgtaaac
     4321 caacaaaagt taatggagga agaacaagaa acttgcctga acatgatctt gaaaaatggc
     4381 aacaatttat agattactgt attcgagatg tagaagtaga aatgacgatt gctcataaaa
     4441 ttaaagactt tccagtaact gcaattgaac aagcatattg ggtttttgac caacatataa
     4501 acgacagagg tattaagctt tctaaatcat tgatgttagg agctaatgtg ctcgataagc
     4561 agagtaaaga agaattgctt aatcaagcta aacatataac aggtttagaa aatcctaata
     4621 gtcctacaca attattggct tggttaaagg atgaccaagg attagatata cctaatttac
     4681 aaaagaaaac ggttcaggag tacttaaaag aagcaacagg aaaagctaaa aaaatgctag
     4741 aaattagatt gcaaatgtct aaaaccagtg tgaaaaaata caacaaaatg catgacatga
     4801 tgtgcagtga tgaacgggta agaggtctgt ttcaatttta cggtgccggt actggaagat
     4861 gggcaggtag aggtgtacaa cttcagaatt taacaaagca ttatatttca gatactgaat
     4921 tagaaatagc aagagatctt attaaagaac aacgttttga cgatttagat ttattactca
     4981 atgttcatcc tcaagactta ttaagtcaat tagttaggac gacatttact gctgaagaag
     5041 gtaatgaact agcagtaagt gatttttctg caatagaggc aagagtcata gcatggtatg
     5101 caaaagaaca atggcgttta gatgtgttca acacacacgg aaagatatat gaagcatcgg
     5161 cttctcaaat gtttaatgtc ccggtagaaa gcataactaa aggcgaccct ctcagacaaa
     5221 agggaaaagt gtccgaatta gctttaggtt atcaaggtgg cgctggagct ttaaaagcga
     5281 tgggcgcatt ggaaatgggc attgaagaaa atgaattaca aggtttagtt gatagttggc
     5341 gtaacgcaaa tcctaacata gttaattttt ggaaggcttg ccaagaggct gcaattaata
     5401 ctgtgaaatc ccgaaagacg catcatacgc atggagttag attttatatg aaaaaaggtt
     5461 ttctaatgat tgaactgcct agtggaagag ctttagctta tccaaaagct tcagttggtg
     5521 aaaatagttg gggtagtcaa gttgttgaat ttatgggctt agatcttaac cgtaaatggt
     5581 caaagttaaa aacgtatggt gggaagttag tcgagaatat tgttcaagca actgcaaggg
     5641 atttacttgc gatttctata gctaggcttg aagcatcagg ttttaaaata gttggacatg
     5701 tccatgatga agtaattgta gaaataccta gaggttcaaa tggacttaag gaaatcgaaa
     5761 ctatcatgaa taagcctgtc gattgggcaa aaggattgaa tttgaatagt gacggattta
     5821 cttctccgtt ttatatgaag gattaggagt gtgattgaat gcaacatcaa gcttatatca
     5881 atgcttctgt tgacattaga attcctacag aagtcgaaag tgttaattac aatcagattg
     5941 ataaagaaaa agagaatttg gcggactatt tatttaataa tccaggtgaa ctattaaaat
     6001 ataacgttat aaatatcaag gttttagatt tagaggtgga atgatggcta gaagaaaagt
     6061 tataagagtg cgtatcaaag gaaaactaat gacattgaga gaagtttcag aaaaatatca
     6121 tatatctcca gaacttctta gatacagata caaacataaa atgcgcggcg atgaattatt
     6181 gtgtggaaga aaagactcaa aatctaaaga tgaagttgaa tatatgaaga gtcaaataaa
     6241 agatgaagaa aaagagagag aaaaaatcag aaaaaaagcg attttgaacc tataccaacg
     6301 aaatgtgaga gcggaatatg aagaagaaag aaagagaaga ttgagaccat ggctttatga
     6361 tggaacgcct caaaaacatt cacgtgatcc gtactggttc gatgtcactt ataaccaaat
     6421 gttcaagaaa tggagtgaag cataatgagc ataatcagta acagaaaagt agatatgaat
     6481 gaaacacaag acaatgttaa acaacctgcg cattacacat acggcgacat tgaaattata
     6541 gatttcatcg aacaagttac ggcacagtat ccaccacaat tagcattcgc aataggtaat
     6601 gcaatcaaat acttgtctag agcaccgtta aagaatggtc atgaggattt agcaaaggcg
     6661 aagttttacg tcgatagagt gtttgacttg tgggaggggt aacgatggca acgcaaaaac
     6721 aagttgatta cgtaatgtca ttacaggagc aactggaatt agaagactgc gaaaaatata
     6781 cagacgaaca agttaaagca atgagtcata aagaagttag caatgtgatt gagaactata
     6841 agacaagcat aaggaatgaa gaactatatt acgaatgcat gtcgtttgga ctgcctaatt
     6901 gttaaaagga gtgacgacca tgacagatag cgcacgtaaa gaacgcttaa accaattttt
     6961 cggctctaaa agatatctgt atcaggataa cgaacgagtg gcacatactc atgtagtaaa
     7021 tggcacgtat tactttcatg ggcatatcgt gccaggttgg caaagcgtta aaaagacatt
     7081 tgatactgct gaagagctcg aaatatatat aaagcaacat ggtttggaat acgaggaaca
     7141 gaagcaacta actttatttt agaggagatg gaaatgatga ataaccgcga acaaattgaa
     7201 caatcaatta tcagtgctag tgcgtataac ggcaatgaca cagagggatt actaaaagag
     7261 attgaagacg tgtataagaa agcgcaagcg tttgatgaaa tacttgaagg tttacctaat
     7321 gctatgcaag atgcactcaa agaagatatt ggtcttgatg aagcagtagg gattatgacg
     7381 gggcaagtgg tctataaata tgaggaggat caggaaaatg actaatacat taacaattga
     7441 tcagttacaa gagttattac aaatacaaaa ggagttcgac gatagaatac caacgctgaa
     7501 cttacgagat agcaaaatag catatgtagt tgaattcttt gaatggttta atacattgga
     7561 aacgtttaag aactggaaga agaaaccagg taagccgtta gatgttcagt tagatgaatt
     7621 agcggacatg ttggcgtttg gattgagtat tgcgaatcaa gtaggagtgt catcagaaga
     7681 gataaaagaa gcgattgaat caagttttaa aaatagtgaa tttcacaaca tgtttaattt
     7741 taaagataaa gaatttgctc aagacgcagt tgttagcaca ccacagataa tattcaaaga
     7801 attttatcct gaccaattgg caattgtaat agcgatagac atagctttca acttatattc
     7861 tatcgaccag ctcattgacg catacaaaaa gaaaatgaaa aggaatcatg aaagacaaga
     7921 tggaacagca gacgcaggaa aaggatacgt gtaaagacat cttagatcga gtcaaggagg
     7981 ttttggggaa gtgacgcaat acttagtcac aacattcaaa gattcaacag gacgcaagca
     8041 tacacacata actaaagcta aaagcaatca aaggtttaca gttgttgagg cagagagtaa
     8101 agaagaagct gagcgcaaat acgaggcaca agttaaaaga gatgcagtta ttaaagtggg
     8161 tcagttattt gaaaatataa gggagtgtgg gaaatgacgg aggttaaaat taaaactatt
     8221 tcagatagag tttattacac aacaacagat ctagcttctg acgattatat taatcttgtt
     8281 atgaatctag tgattgagga ttttcttccg gtcaaagatg tgttcaacaa tgaagtatgg
     8341 gttaaaagag atgagattga atcatttaca tttattaagg aggcaaacga tgattaacat
     8401 acctaaaatg aaattcccga aaaagtacac tgaaataatc aaaaaatata aaaataaagc
     8461 acctgaagaa aaggctaaga ttgaagatga ttttattaaa gaaattaaag ataaagacag
     8521 tgaattttac agtcctacga tggctaatat gaatgaatat gaattaaggg ctatgttaag
     8581 aatgatgcct agtttaattg atactggaga tgacaatgat gattaaaaaa cttaaaaata
     8641 tggatgggtt cgacatcttt attgttggaa tactgtcatt attcggtata ttcgcattgc
     8701 tacttgttat cacattgcct atctatacag tggctagtta ccaacacaaa gaattacatc
     8761 aaggaactat tacagataaa tataacaaga gacaagataa agaagacaag ttctatattg
     8821 tattagacaa caaacaagtc attgaaaatt ccgacttatt attcaaaaag aaatttgata
     8881 gcgcagatat acaagctagg ttaaaagtag gcgataaggt agaagttaaa acaatcggtt
     8941 atagaataca ctttttaaat ttatatccgg tcttatacga agtaaagaag gtagataaac
     9001 aatgattaaa caaatactaa gactattatt cttactagca atgtatgagt taggtaagta
     9061 tgtaactgag caagtgtata ttatgatgac ggctaatgat gatgtagagg cgccgagtga
     9121 ttacgaaaaa atcagagctg aagtttcatg gtaatagcta ttatcatttt tgaattaatt
     9181 atattaatgt gtttagcaat agcactggag gtgttgtaaa tatgtggatt gtcatttcaa
     9241 tcgttttagc tatattttta ttgatcttgt taagtagcat ttctcataag atgaaaacca
     9301 tagaagcatt ggagtatatg aattcttatc ttttcaagca gttagtaaaa aataatggtg
     9361 ttgaaggttt agaagattat gaaaatgaag ttgaacgaat tagaaaaaga ttcaaaagct
     9421 aaagaggggg ctaaagccct ctccaggatt aaaagaagcg gatatttttt atttcgtttt
     9481 tgtaaattaa aactaattta tgtgaaattg tctttgaagt cttgataatt aagaaggttg
     9541 aattttctga gattatatta gttacgggaa aggcttttcc tgagtgcaat aaaattaaag
     9601 ttctcaagtt ttcattttcg tatttatcat aaatgatttc gcgtaaagtt ttcaaaattt
     9661 gtcacctctt taattttatt aaattaatta tacaagaaag gagccgaata tgttagacaa
     9721 agtcactcaa atagaaacaa ttaaatatga tcgtgatgtc tcatattctt atgctgctag
     9781 tcgtttatcc acatattgga ctaatcacaa tatggcttgg tctgacttta tgcagaagct
     9841 agcacaaaca gttagaacta aagaagattt aactgagtac aataaaatgt ctaagtctga
     9901 acaagcagat ataaaagatg ttggcggatt tgtcggtgga tatttaaaag aaggcaaacg
     9961 acgtgctggt caagtcatga atcgttcaat gctaacactt gatatcgatt atgctgctca
    10021 agatatgact gacatattat ctatgtttta tgattttgca tattgtttat attcaacaca
    10081 taagcataga gagataagtc caagactgcg tttagtgatt cctttaaaac gaaatgtaaa
    10141 tgcagatgag tatgaagcta ttgggcgtaa agtcgcagat atcgttggca tggattactt
    10201 cgatgataca acttatcaac cacataggtt aatgtattgg ccttcaacta gcaatgatgc
    10261 agaatttttc tttacctatg aagatttacc tttgttagat ccagataaaa tattaaatga
    10321 atatgttgat tggactgaca cattagaatg gccaacgtct tcaaaggaag agagtaagac
    10381 taaaagatta gcagataagc aaggtgaccc agaagaaaag ccgggaattg ttggcgcatt
    10441 ttgtagagcc tatacgatag aagaagctat atcaactttt attcctgact tatacgaaaa
    10501 acattctact aaccgttata cctatcatga aggttcaact gcaggtggat tggtgttata
    10561 cgaaaataac aagtttgcct attctcatca taatacggat cccgttagcg gtatgcttgt
    10621 gaacagtttt gatttagtac gcatacactt atatggtgct caagatgaag acgctaaaac
    10681 agatactccg gttaatcgac tacctagtta taaagcaatg cagcaaagag cgcaaaatga
    10741 tgaagttgtt aaaaagcaat taattaacga caaaatgtct gatgcaatgc aggatttcga
    10801 tgaaatagta aatagcgatg atgcatggtc tgagacgtta gaaattactt cgaaaggtac
    10861 tttcaaagct agtatcccaa atatagaaat tatattgcgt aatgatccaa atttaaaagg
    10921 aaaaatagca tttaatgaat ttacaaaaca aattgaatgc ttagggaaag tgccatggaa
    10981 tactaatttt aagacacgtc aatggcaaga cggtgatgat agcagtttaa gaagttatat
    11041 cgaaaagatt tatgacatac accattcagg taaaacaaaa gatgccatta taagcgtagc
    11101 aatgcaaaat gcttatcatc cagtaaggga ttatctaaat aaaatatcgt gggatggaca
    11161 taaacgtctt gaaaagttat ttatcaaata cttaggtgtt gaagatactg aagtgaatag
    11221 aacaactacc aaaaaagcat tgactgctgg aattgctcga gtaatggagc ctggatgtaa
    11281 atttgactat atgcttacac tttatggtcc tcaaggtgta ggtaaatctg ctttgctaaa
    11341 aaaattaggt ggtgcatggt tttctgacag tttagtttct gttacaggta aagaagccta
    11401 tgaggcctta caaggcgttt ggctaatgga aatggcagaa cttgcagcta caagaaaagc
    11461 tgaagttgaa gctattaagc atttcatatc taaacaagtt gaccggtttc gtgttgctta
    11521 tggacattat attgaagatt ttccaaggca atgtattttc attggtacaa ctaataaagt
    11581 tgatttctta agagatgaaa ctggtggaag acgtttttgg ccaatgactg taaatccaga
    11641 gagagttgaa gtgaactggt ctaaactaac caaagaagag atcgaccaaa tttgggcaga
    11701 agctaaatat tattatgaac aaggagaaga gttattcctc aaccctgaac tagaagaaga
    11761 aatgcgttca atacaaagca aacatactga ggaatctcca tatacaggca ttattgatga
    11821 atatcttaac acaccaattc ctagcaattg ggatgactta actatctttg aacgaagacg
    11881 attttatcaa ggtgatgttg atatgttacc aacaggaaat gtagattacg ttgaaagaaa
    11941 taaggtctgt gcgcttgaag tgtttgttga atgttttggt aaagataagg gagatagtag
    12001 aggatctatg gaaattagaa agatttcaaa catcttaaga caattagaca attggtctgt
    12061 atatgatggt aataaaagtg ggaaaattcg atttggaaaa gattatggtg tacagatagc
    12121 ttatgtaaga gatgaaagtt tagaagattt aatataataa atattgaata aatatacatt
    12181 ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga tgcaacacgt gagtgtaaaa
    12241 agtaatcgta ggtgttgcat catttttagt gatgcaacat tgatgcaaca aatgatacaa
    12301 cacctctttc ccttctcgct gtaaggttca accctgtttg tttccaatgt tgcatcaaat
    12361 tcactataaa gtttaaaaag tagtgttagg gagtaaagag gtataggggt aaccctctaa
    12421 cagctatttt taaaagtttg gcaagaattg atgcaacatc ggaacacaaa tataaatttt
    12481 gtatacaagg tgaatatatg aaagaatcga cattagaaaa atatttagtg aaagagatat
    12541 caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg aacaagaggt gtgccagata
    12601 gaattattat tatgccagaa ggaaaaacat attttgtaga aatgaagcaa gaaaaaggaa
    12661 agttgcatcc tttacaaaaa tatgtgcata gacaatttga aaatagagat cataaagtat
    12721 atgtgttatg gaataaagaa caagtaaata cttttatcag aatggtaggt ggaacatttg
    12781 gcgattgact tcaaaccaca tagctatcaa aagtatgcaa tagataaagt gatagataat
    12841 gagaaatacg gtctgttttt agatatgggt ctagggaaaa cagtatcaac acttacagca
    12901 tttagtgaat tgcagttgtt agacactaaa aaaatgttag ttatagcacc taaacaagtt
    12961 gctaaagata catgggttga tgaagttgat aagtggaacc atttaaatca tctgaaagtg
    13021 tctttagttt taggaacacc taaagaaaga aatgatgcat taaacacaga ggctgatatc
    13081 tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc aatataaaaa agaatggcca
    13141 tttgacatgg ttgtgattga tgaactgtct acatttaaaa gtcctaagag tcaaaggttt
    13201 aaatctatta aaaagaaatt accactcatt aatagattta taggattaac aggaacacct
    13261 agtccaaata gtttacagga tttatgggct caagtttatt tgatagacag aggtgaaaga
    13321 cttgagtctt cattcagtcg ttatcgagaa aggtacttta aaccaactca tcaagttagc
    13381 gaacatattt ttaagtggga gctaagagac ggatctgaag aaaagatata taaacaaata
    13441 gaagatatat gtttaagcat gaaagcgaaa gattatctgg atatgcctga cagagttgat
    13501 actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag tatatgaaga attagaaaaa
    13561 aactatattt tagaatcgga agaagaagga acagttgtag ctcaaaatgg ggcatcatta
    13621 agtcagaaac tacttcaact atctaacggc gcagtttata cagatgagga agatgtaaga
    13681 cttatacatg ataagaagtt agataagtta gaggaaatta tagaggagtc tcaaggccaa
    13741 ccaatactat tgttttataa cttcaaacac gataaagaaa gaatacttca aaggtttaag
    13801 gaagcaacca cattagacga ttcaaactat aaagaacgtt ggaacagtgg agacattaag
    13861 ttgcttatag cacatccagc aagtgcagga catggattaa acttacaaca aggtgggcac
    13921 attattgttt ggtttggact tacatggtcc ttggaattat accaacaagc aaatgctaga
    13981 ttatacagac aaggacaaaa tcatacgact attattcatc atatcatgac cgataacaca
    14041 atagatcaaa gagtatataa agctttacaa aataaagaac taacgcaaga agaattaatg
    14101 aaagctatta aagcaagaat agctaagcat aagtaatgga ggtctaacat ggggaacaca
    14161 atatatgata tcaagccagg cacatttaaa tatattgaat cagaaatata taatttaaat
    14221 gagaacaaga aagaaataaa aagattgagg ttggaaatac ttaatccaac gaaggaacaa
    14281 gattccaata ttgtatatgg accattacaa aaaggcgaac cagttagaac aactgaacta
    14341 atggcaacta gattattaac taataagatg ttacgaaacc ttgaagaaat ggtcgaagca
    14401 gttgagagtg aatacttaaa gttgcctgaa gatcataaga aagtaataag gctcaagtat
    14461 tggaataaag aaaagaagtt aaagatggaa cagataggac atgaatgcca tatgcatcgt
    14521 aatactgtta ctactatacg aaagaacttt gttaaagcgg tagcgtatca tgcaggtatc
    14581 aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat ctgtagtaat atgatagtat
    14641 cggaaagatg tataaagtta tctaaaagtt atacgacaca agtacacgag gcacatcgct
    14701 atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag agatgaccaa gcacaataac
    14761 atttataagc atggtcgtaa gtcatatcaa tacgattggt tctatcattc aaaagcatgg
    14821 aagaagttaa gggagatagc attagataga gataatcatc tttgtcaaat gtgtttacgc
    14881 gaagatattg taacagatgc aaacatagtg catcacatta tttatgttga tgaagatttt
    14941 aacaaagctt tagaattaga taatctaatg tcagtttgtt atggctgtca taacaaaatt
    15001 catgcaaatg gtaatgacaa aagtaatctt aagaaaatta gagttctaaa aatttaaata
    15061 aaaaaacatt taaataaaat tttatagccc cctgcccatt ggcttaaaat gttttttcgc
    15121 cgggtaccgg cgggggccct tcgcttgcaa cgcggataaa cttttatgaa agggggtctt
    15181 tatatgaaat taacaaaaaa acagctgaaa gaatatatag aggattataa aaaatctgat
    15241 gacatattaa ttaatttgta tatagaaacg tatgaatttt attgtcggtt aagagatgaa
    15301 cttaaaaata gtgatttgat gatagagcat acaaacaagg ctggtgcgag caatattgtt
    15361 aagaatccat taagcataga actgacaaaa acagttcaaa cactaaataa cttactcaag
    15421 tctatgggtt taactgcagc acaaagaaaa aagatagttc aagaagaagg tggattcggt
    15481 gactattaaa gttttaaatg aaccttcacc aaaactatta acaacatggt atgcagagca
    15541 agtcactcaa gggaaaataa aaacaagcaa atatgttaaa aaagaatgtg agagacacct
    15601 tagatatcta gaaaatggag gtaaatgggt atttgatgaa gaattagcgc accgtcctat
    15661 tcgattcata gaaaagtttt gtaaaccttc caaaggatct aaacgtcaac ttgtattaca
    15721 accatggcaa cattttatta ttggcagttt gtttggttgg gttcataaag aaacaaaact
    15781 gcgcaggttt aaagaagctt tgatatttat ggggcgaaaa aatggtaaaa caactactat
    15841 atctggtgtt gctaactatg ctgtttctca agatggagaa aacggcgctg aaatccatct
    15901 tttagcaaac gtaatgaaac aagctagaat attattcgat gaatctaagg cgatgataaa
    15961 agctagccca aagcttgata aaaatttcag aacattaaga gatgaaatcc attatgacgc
    16021 aacgatatca aaaattatgc cccaagcatc agatagcgat aagttagatg gattgaatac
    16081 acacatgggg atttttgatg aaattcatga atttaaagac tataaattga tttcagttat
    16141 aaaaaactca agagctgcaa ggttacaacc tcttctcatc tacattacga cagcagggta
    16201 tcaattagat ggtccacttg ttgatatggt agaagcggga agagacacct tagatcaaat
    16261 catagaagac gaaagaactt tttattattt agcatctttg gatgatgacg atgatattaa
    16321 tgattcgtcg aactggataa aagcaaatcc caacttaggt gtctctataa atttagatga
    16381 gatgaaagaa gagtgggaaa aagctaagag aacaccagct gaacgtggag attttataac
    16441 caaaaggttt aatatctttg ctaataatga cgagatgagt tttattgatt acccaacact
    16501 ccaaaaaaat aatgaaattg tttctttaga agagctggaa ggcagaccat gcacgattgg
    16561 ttatgattta tcagaaacag aggactttac agccgcgtgt gctacttttg cgttagataa
    16621 tggtaaagtt gcagttttat cgcattcatg gattcctaag cacaaagttg aatattctaa
    16681 cgaaaaaata ccctatagag aatgggaaga agatggctta ttaacagtgc aagataagcc
    16741 ttatattgac taccaagatg ttttaaattg gataattaag atgaatgagc attatgtagt
    16801 agaaaaaatt acttatgata gagcgaacgc attcaaacta aatcaagagt taaaaaatta
    16861 cgggtttgaa acggaagaaa caagacaagg agctttgacc ttgagccctg cattgaagga
    16921 tttaaaagaa atgtttttag atgggaaaat aatatttaat aataatcctt taatgaaatg
    16981 gtatatcaat aatgttcagt tgaaactaga cagaaacgga aactggttgc cgtctaagca
    17041 aagcagatat cgtaaaatag atggctttgc agcattttta aacacatata cagatattat
    17101 gaataaagtt gtttctgaca agggtgaagg aaacatagaa tttattagta ttaaagatat
    17161 aatgcgttaa ggaggtgaat gttatcgcaa aagagaatat tgtcacacgc ataaagaaaa
    17221 aattgataga caattggatt gatcagtcag cttctaagct ttatgacttt agcccatgga
    17281 aaaataaatc tttttggggt gtaatcaata atacgcttga aactaatgaa acgatatttt
    17341 cagctattac aaagttatct aattcgatgg ctagtttgcc cttgaaaatg tatgaagatt
    17401 ataaagtagt taatacagaa gtatctgatt tacttacagt gtcaccgaat aattctctga
    17461 gcagttttga ttttattaat caaattgaaa caatcagaaa tgaaaaaggt aatgcatatg
    17521 tgctaattga acgagacatc tatcatcaac catcaaagct tttcttatta aatccagatg
    17581 ttgttgaaat gttaattgaa aaccaatcac gtgaacttta ttattccatt catgctgcaa
    17641 ctggaaataa attgattgtt cataatatgg acatgttgca ttttaaacac atcgtggcat
    17701 ctaatatggt gcaaggcatt agtccgattg atgtgttgaa gaatacaact gattttgata
    17761 atgcagtaag aacctttaat cttacagaaa tgcaaaaacc tgattctttc atgcttaaat
    17821 atggttccaa tgtaggtaaa gaaaaaaggc agcaagtgtt agaagatttc aaacagtact
    17881 atgaagaaaa cggtggaata ttattccaag agcctggtgt tgaaatcgaa ccgttaccta
    17941 aaaaatatgt ctctgaagat atagtggcaa gcgagaattt aacaagagaa agagtagcta
    18001 acgtttttca attgccctca gtattcttaa atgcaagatc aaatacaaat ttcgcgaaaa
    18061 atgaagagtt aaacagattt tacttgcagc ataccttatt gccaatcgtc aaacagtatg
    18121 aagaagaatt taatcggaaa ctacttacta aaacagacag agaaaaaaat aggtatttta
    18181 aatttaacgt taaatcttat ttaagggctg atagtgcaac acaagcagaa gtgtacttta
    18241 aagcagttcg tagtggttac tacactataa atgacattag agagtgggaa gatttaccac
    18301 cagttgaagg tggagataag ccgctaataa gcggtgattt atacccaatt gacacgccac
    18361 ttgaattaag aaaatctttg aaaggtggtg ataaaaatgt caatgaaagc taagtatttt
    18421 caaatgaaaa gaaaatcaaa aagtaaaggt gaaatattta tttatggtga tattgtaagt
    18481 gataaatggt ttgaaagtga tgtaactgct acagatttca aaaataaact agatgaacta
    18541 ggagacatca gtgaaataga tgttcatata aattcatctg gaggcagtgt atttgaaggg
    18601 catgcaatat acaatatgct aaaaatgcat cctgcaaaaa ttaatatcta tgtcgatgcc
    18661 ttagcggcat caattgctag tgttatcgct atgagtggtg acactatttt tatgcacaaa
    18721 aatagttttt taatgattca taattcatgg gttatgactg taggtaatgc agaagaatta
    18781 agaaagacag cggatttact tgaaaaaaca gatgctgtta gtaattcagc ttatttagat
    18841 aaagcaaaag atttagatca agaacactta aaacagatgt tagatgcaga aacttggctt
    18901 actgcagaag aagccttgtc tttcggcttg atagatgaaa ttttaggagc taatgaaata
    18961 gctgctagta tctctaaaga gcaatataag cgtttcgaga acgtcccaga agatttaaag
    19021 aaagatgtag acaaaatcac taaaattgat gatgtagata catctgaatt ggttgaaaca
    19081 cctaaagaaa gtatgtcact agaagaaaaa gaaaaaagag aaaaaattaa acgcgaatgc
    19141 gaaattttaa aaatgacaat gaattattag gaggaaatga aatgccgaca ttatatgaat
    19201 taaaacaatc cttaggtatg attggacaac aattaaaaaa taaaaatgat gaattgagtc
    19261 agaaagcaac agatccaaat attgatatgg aagacatcaa acaactagaa acagaaaaag
    19321 caggtttaca acaaagattt aacattgttg aaagacaagt gcaagacatt gaagagaaag
    19381 aaaaagcgaa agttaaagat aaaggagaag cttatcaatc tttaagtgat aatgagaaga
    19441 tggttaaagc taaggcagag ttttatcgtc acgcgatttt accaaatgaa tttgaaaaac
    19501 cttcaatgga ggcacaacgt ttattacacg ctttaccaac aggaaatgat tcaggtggag
    19561 ataagctctt accaaaaaca ctttctaaag aaattgtttc agaaccattt gctaaaaacc
    19621 aattacgtga aaaagctcgt ctaactaaca ttaaaggttt agagattcca agagtttcat
    19681 acactttaga cgatgatgat ttcattacag acgtagaaac agcaaaagaa ttaaaagcaa
    19741 aaggtgatac agtcaagttc actactaata aattcaaagt atttgctgca atttcagata
    19801 ctgtaattca tggatcagat gtagatttag taaactgggt tgaaaacgca ctacaatcag
    19861 gattagcagc taaagagcgt aaagatgcct tagcagtaag tcctaaatct ggattagaac
    19921 acatgtcatt ttataatgga tctgttaaag aagttgaggg agcagacatg tatgatgcta
    19981 ttattaacgc tttagcagat ttacatgaag attatcgtga taacgcaaca atttatatgc
    20041 gatatgcaga ttatgtcaaa attattagtg ttctttcaaa tggaacaaca aatttctttg
    20101 acacaccagc agaaaaagta tttggcaaac cagtagtatt tacagatgca gcagttaaac
    20161 ctattgtggg agatttcaat tattttggaa ttaactatga tggaacaact tatgacactg
    20221 ataaagatgt taaaaaaggc gaatatttgt ttgtattaac agcatggtat gatcagcaac
    20281 gtacattaga cagtgcattc agaattgcaa aagcaaaaga aaatacaggt ccattaccca
    20341 gctaagcccc aaaaggttaa tgtaacagct aaggctaaat cagctgtaat atcagccgaa
    20401 taggggtgat gaaatgagtt tagaagaaat taaattgtgg ttgagaattg actataattt
    20461 cgaaaatgat ttaattgaag gtctcattca atcggctaag tctgaattac tattaagtgg
    20521 ggttccagat tatgacaaag atgacttgga atacccgctt ttttgtacag cgattaaata
    20581 tatcattgca agagattatg aaagtcgtgg atactcaaat gaccaatcta gaagcaaggt
    20641 gtttaatgaa aaaggattgc aaaaaatgat tttgaaatta aaaaagtggt aggtgatttt
    20701 taaatggaat ttaatgaatt taaagatcgc gcgtattttt ttcaatatat aaacaaagga
    20761 ccatatccag atgaagagga aaaaatgaaa ttgtatagtt gcttttgtaa aatttataat
    20821 ccttctatga aagatagaga aattttaaaa gcgactgaat caaaatcagg attaaccata
    20881 attgtcaggt cttctaaaac tgaatatcta ccacaaacaa atcacttagt taaaattgac
    20941 agtgcattat attccgataa attattcaac attgtagaaa taagaattga tacaccagat
    21001 attggctata atacagtggt tttatcagaa aaatgagtgt agaaattaaa gggatacctg
    21061 aagtgttgaa taaattagaa tcggtatacg gtaaacaagc aatgcaggct aagagtgata
    21121 aagctttaaa tgaagcatct gaatttttta taaaggcttt aaagaaagag ttcgagagct
    21181 ttaaagatac gggtgccagt atagaagaaa tgactaaatc taagccttat acaaaagttg
    21241 gcagtcaaga aagggctgtt ttaattgaat gggtaggccc tatgaatcgc aaaaacatta
    21301 ttcacttgaa tgaacatggt tatacaagag atggaaaaaa atatacacca agaggttttg
    21361 gagttattgc aaaaacatta gctgctagcg aacgtaagta tagagaaatt ataaaaaagg
    21421 agttggccag ataaatgaat atattaaaca ccataaaagg aattttatta tctgatgcag
    21481 agctcaaaac acatataaat tctagaatat actattacaa agtcactgaa aacgctgaaa
    21541 cttccaaacc ctttgttgtt attacacctg tttatgattt gccttcagac tttatgtctg
    21601 ataaatatct cagtgaagaa tacttaattc aaatagatgt agaatcttca aataatcaga
    21661 aaacaattga tataacaaaa cgaataagat acctgttata tcaacaaaat ttaattcaag
    21721 catctagtca gttagatgct tattttgaag aaactaaacg ttatgtgatg tcgagacgat
    21781 atcaaggcat acccaaaaat atatattata aaaatcagcg catcgaatag gtgtgctttt
    21841 taatttttaa ggaggaaata agcaatggca gaaggacaag gttcttataa agtaggtttt
    21901 aaaagattat acgttggagt ttttaaccca gaagcaacaa aagtagttaa acgcatgaca
    21961 tgggaagatg aaaaaggtgg tacagttgac ctaaatatca caggtttagc accagattta
    22021 gtagatatgt ttgcatctaa caaacgtgta tggatgaaaa aacaaggtac taatgaagtt
    22081 aagtctgaca tgagtatttt caatattcca agtgatgatt taaacacagt tattggacgt
    22141 actaaagata aaaatggtac atcttgggta ggagagaata caagagcacc gtatgtaaca
    22201 gtaattggcg aatcggaaga tggtttaaca ggtcagccgg tatatgtagc cttacttaaa
    22261 ggtactttta gtttagattc aattgaattt aaaacacgag gtgaaaaagc agaagcccca
    22321 gaacctacaa aattaacagg tgactggatg aatagaaaag ttgatgttga tggaacgtca
    22381 caaggtattg tatacggtta tcatgaaggt aaagaaggag aagcagaatt cttcaaaaaa
    22441 gtattcgttg gatacacgga cagtgaagat cattcagagg attctgcagg ttcgttaccc
    22501 agctaatccc caaaatgttg aagtagcagt taattcaaaa tctgcaacag tttcagcaga
    22561 ataggggctt tcaaaataaa tcaaaggaga ataatttatg actaaaactt taaaggttta
    22621 taaaggagac gacgtcgtag cttctgaaca aggtgaaggc aaagtgtcag taactttatc
    22681 taatttagaa gcggatacaa cttatccaaa aggtacttac caagtggcat gggaagaaaa
    22741 tggtaaagaa tctagtaaag ttgatgtacc tcaattcaaa accaatccaa ttctagtctc
    22801 aggcgtatca tttacaccag aaactaaatc aattatggta aataccgatg acaatgttga
    22861 gccaaacatt gcaccaagca cagcaacgaa taaaatattg aaatatacaa gtgaacatcc
    22921 agaatttgtt actgtagatg aaaatacagg agcaattcac ggtgtagctg aaggtacttc
    22981 agtaatcact gctatgtcta ctgatggaag cgataagtca ggacaaattt cagtgacagt
    23041 aacaaacgga tagggattta aggcgcagta tatctgcgtc ttttttattt gaataaaagg
    23101 agctaataca atgattaaat ttgaaattaa agatcgtaaa acaggaaaaa cagagagcta
    23161 tacaaaagaa gatgtaacaa tgggcgaagc agaaaaatgc tatgagtatt tagaattagt
    23221 aaatcaagag aataaaaaag aagcacctaa cgcaacaaaa atgagacaaa aagagcgaca
    23281 gttattagta gatttattta aagatgaagg attgactgaa gaagatgttc tgaacaagat
    23341 gagtactaaa acttatacaa aagccttaca agatatattt cgagaaatca atggtgaaga
    23401 tgaagaagat tcagaaactg aaccagaaga gatgggaaag acagaagaac aatctcaata
    23461 aaagacattt tatcgaacat taagaaaata caacgtttct gtatggagca gtatgggtgg
    23521 acattaactg aagtcagaaa acagccgtat gtaaaacttt tagaaatact taatgaagag
    23581 aataaagaag agactgaaga aaaacaaagt gaacaaaaag tcattacagg tacggattta
    23641 agaaaacttt ttggaagcta gaaaggaggt taatatgaat gaaaaagtag aaggcatgac
    23701 cttggagctg aaattagacc atttaggtgt ccaagaatgc atgaaaggtt taaagcgaca
    23761 attaggtgtt gttaatagtg aaatgaaagc taatctgtca gcatttgata agtctgaaaa
    23821 atcaatggaa aaatatcagg cgagaattaa ggggttaaat gataggctta aagttcaaaa
    23881 aaagatgtat tctcaagtag aagatgagct taaacaagtt aacgctaatt accaaaaagc
    23941 taaatccagt gtaaaagatg ttgagaaagc atatttaaag ttagtagaag ccaataaaaa
    24001 agaaaaatta gctcttgata aatctaaaga agccttaaaa tcatcgaata cagaacttaa
    24061 aaaagctgaa aatcaatata aacgtacaaa tcaacgtaaa caagatgcgt atcaaaaact
    24121 taaacagttg agagatgcag aacaaaagct taagaatagt aaccaagcta ctactgcaca
    24181 actaaaaaga gcaagtgacg cagtacagaa gcagtccgct aagcataaag cacttgttga
    24241 acaatataaa caagaaggca atcaagttca aaaactaaaa gtgcaaaatg acaatctttc
    24301 aaaatcaaat gataaaattg aaagttctta cgctaaaact aatactaaat taaagcaaac
    24361 agaaaaagaa tttaatgatt taaacaatac tattaagaat catagcgcta atgtcgcaaa
    24421 agctgaaaca gctgttaata aagaaaaagc tgctttaaat aatttggagc gttcaataga
    24481 taaagcttca tccgaaatga agacttttaa caaagaacaa atgatagctc aaagtcattt
    24541 cggtaaactt gcaagtcaag cggatgtcat gtcaaagaaa tttagttcta ttggagacaa
    24601 aatgacttcc ctgggacgta caatgacgat gggcgtatct acaccaatta ctttagggtt
    24661 aggtgcagca ttaaaaacaa gtgcagactt tgaaggccaa atgtctcgag ttggagcgat
    24721 tgcgcaagca agcagtaaag acttgaaaag catgtctaat caagcagttg acttaggagc
    24781 taaaaccagt aaaagtgcta acgaagttgc taaaggtatg gaagaattgg cagctttagg
    24841 ctttaatgcc aaacaaacaa tggaggctat gccaggtgtt atcagtgcag cagaagcaag
    24901 tggtgcagaa atggctacaa ctgcaactgt aatggcttca gcgattaact ctttcggttt
    24961 aaaagcatct gatgcaaatc atgttgctga tttacttgcg agatcagcaa atgatagtgc
    25021 tgcagatatt cagtacatgg gagatgcatt gaagtatgct ggtactcctg caaaagcatt
    25081 aggagtttca atagaggaca cttccgcagc aattgaagtt ttatctaact caggtttaga
    25141 gggttctcaa gcaggtactg ccctaagagc ttcatttatc aggctagcta atccaagtaa
    25201 aaatacagct aaggaaatga aaaaattagg tattcatttg tctgatgcta aaggtcaatt
    25261 tgttggcatg ggtgaattga ttagacagtt ccaagataat atgaaaggca tgacgagaga
    25321 acaaaaacta gctacagtgg ctacaatagt tggtactgaa gcagcaagtg gatttttagc
    25381 cttgattgaa gcgggaccag ataaaattaa tagctatagt aaatccttaa agaattccaa
    25441 tggcgaaagt aaaaaagcag cagatttgat gaaagataat ctcaaaggcg ctctggaaca
    25501 attaggtggc gcttttgaat cattagcaat cgaagtcggt aaagatttaa cgcctatgat
    25561 tagagcagga gcggaaggtt taacaaaatt agttgatgga tttacacatc tccctggttg
    25621 ggttagaaaa gcttcagtag gattagcact ttttggtgca gcaattggac ctgcagttct
    25681 tgctggaggg ttattaatac gtacagttgg aagtgctgct aaaggatatg cgtcattaaa
    25741 tagacgtatt gctgaaaata caatcctttc aaatactaat tcaaaagcaa tgaaatcttt
    25801 aggtcttcaa acattatttc ttggttctac aacaggaaaa acgtcaaaag gctttaaagg
    25861 gttagccgga gctatgatgt ttaatttaaa acctataaat gttttgaaaa attctgcaaa
    25921 gctagcaatt ttaccgttca aacttttgaa aaacggttta ggattagctg caaaatcttt
    25981 atttgcagta agtggaggcg caagatttgc gggtgtagcc ttaaggtttt taacaggacc
    26041 tataggtgct acaataactg ctattacaat tgcgtataaa gtttttaaaa ccgcatatga
    26101 tcgtgtggaa tggttcagaa acggtattaa cggtttagga gaaactataa agttttttgg
    26161 tggtaaaatt attggcggcg ctgttagaaa gctaggagag tttaaaaact atcttggaag
    26221 tatcggcaaa agcttcaaag aaaagttttc aaaagatatg aaagatggtt ataaatcatt
    26281 aagcgacgat gaccttctca aagtaggagt caacaagttt aaaggattta tgcaaaccat
    26341 gggcacagct tctaaaaaag cgtctgatac tgtaaaagtg ttagggaaag gtgtttcaaa
    26401 agaaacagaa aaagctttag aaaaatatgt gcattattct gaagaaaata gcagaatcat
    26461 ggaaaaagta cgtttaaact cgggtcagat atcagaagac aaagcaaaaa aacttttgaa
    26521 aattgaaacg gatttatcta ataaccttat agctgaaata gaaaaaagaa ataaaaagga
    26581 actcgaaaaa actcaagaac ttattgataa gtatagtgca ttcgatgaac aagaaaagca
    26641 aaacatttta actcgaacta aagaaaaaaa tgacttgcga attaaaaaag agcaagaact
    26701 caatcagaaa atcaaagaat tgaaagaaaa agctttgagt gatggtcaga tttcagaaaa
    26761 tgaaagaaaa gaaattgaaa agcttgaaaa tcaaagacgt gatatcactg ttaaagaatt
    26821 gagtaagact gaaaaagagc aagagcgtat tttagtaaga atgcaaagaa acagaaatgc
    26881 ttattcaata gacgaagcga gcaaagcaat taaagaagca gaaaaagcaa gaaaagcaag
    26941 aaaaaaagaa gtggacaagc aatatgaaga tgatgtcatt gctataaaaa ataacgtcaa
    27001 cctttctaag tctgaaaaag ataaattgtt agctattgct gatcaaagac ataaagatga
    27061 agtaagaaaa gcaaaatcta aaaaagatgc tgtagtagat gttgttaaaa agcaaaataa
    27121 agatattgat aaagaaatgg atttatccag tggacgtgta tataaaaata ctgaaaagtg
    27181 gtggaatggc cttaaaagtt ggtggtctaa ctttagagaa gaccaaaaga aaaaaagcga
    27241 taaatacgct aaagaacaag aagaaacagc tcgtagaaac agagaaaata taaagaaatg
    27301 gtttggaaat gcttgggacg gcgtaaaaag taaaactggc gaagccttta gtaaaatggg
    27361 cagaaatgct aatcattttg gcggcgaaat gaaaaaaatg tggagcggaa tcaaagggat
    27421 tccaagcaaa ttaagttcag gttggagctc agccaaaagt tctgtaggat accacactaa
    27481 ggctatagct aatagtactg gtaaatggtt tggaaaagct tggcaatctg ttaaatcgac
    27541 aacaggaagt atttacaatc aaactaagca aaagtattca gatgcttcag ataaagcttg
    27601 ggcgcattca aaatctattt ggagaggcac atcaaaatgg tttagcaatg catataaaag
    27661 tgcaaagggc tggctaacgg atatggctaa taaatctcgc gcgaaatggg ataatatttc
    27721 tagtacagct tggtcgaatg caaaatccgt ttggaaagga acatcgaaat ggtttagtaa
    27781 ctcatacaaa tctttaaaag attggactgg ggatatgtat tcaagagccc acgatcgttt
    27841 tgatgcaatt tcaagttcgg catggtctaa cgctaaatca gtatttaatg gttttagaaa
    27901 atggctatcc aaaacatatg attggattag agatattggt aaagacatgg gaagagctgc
    27961 ggctgattta ggtaaaaatg ttgctaataa agctattggc ggtttgaata gcatgattgg
    28021 cggtattaat aaaatatcta aagccattac tgataaaaat ctcatcaagc caatacctac
    28081 attgtctact ggtactttag caggaaaggg tgtagctacc gataattcgg gagcattaac
    28141 gcaaccgaca tttgctgtat taaatgatag aggttctgga aacgccccag gtggtggagt
    28201 tcaagaagta attcacaggg ctgacggaac attccatgca ccccaaggac gagatgtggt
    28261 tgttccacta ggagttgggg atagcgtaat aaatgctaat gacactctga agttacagcg
    28321 tatgggtgtt ttaccaaagt ttcatggagg tacgaaaaag aaagattggc tagaccaact
    28381 taaaggtaat ataggtaaaa aagcaggaga atttggagct acagctaaaa acacagcgca
    28441 taatatcaaa aaaggtgcag aagaaatggt tgaagcagca ggcgataaaa tcaaagatgg
    28501 tgcatcttgg ttaggcgata aaatcggcga tgtgtgggat tacgtacaac atccagggaa
    28561 actagtaaat aaagtaatgt caggtttaaa tattaatttt ggaggcggag ctaacgctac
    28621 agtaaaaata gctaaaggcg catactcatt gctcaaaaag aaattagtag acaaagtaaa
    28681 atcgtggttt gaagatttcg gtggtggagg cgatggaagc tatctatttg aatatccaat
    28741 ctggcaaaga tttggacgct acacaggtgg acttaacttt aatggcggtc gtcactatgg
    28801 tatagacttt ggtatgcctt ctggaacaaa cgtttatgcc gttaaaggtg gtatagcaga
    28861 taaggtatgg actgattacg gtggcggtaa ttctatacaa attaagactg gtgctaatga
    28921 atggaactgg tatatgcatt tatctaagca attagcaaga caaggccaac gtattaaagc
    28981 tggtcaactg atagggaaat caggtgctac aggtaatttc gttagaggag cacacttaca
    29041 tttccaattg atgcaagggt cacatccagg gaatgataca gctaaagatc cagaaaaatg
    29101 gttgaagtca cttaaaggta gtggcgttcg aagtggttca ggtgttaata aggctgcatc
    29161 tgcttgggca ggcgatatac gtcgtgcagc aaaacgaatg ggtgttaatg ttacttcggg
    29221 tgatgtagga aatattatta gcttgattca acacgaatca ggaggaaatg caggtataac
    29281 tcaatctagt gcgcttagag acatcaacgt tttacagggc aatccagcaa aaggattgct
    29341 tcaatatatc ccacaaacat ttagacatta tgctgttaga ggtcacaaca atatatatag
    29401 tggttacgat cagttattag cgttctttaa caacagatat tggcgctcac agtttaaccc
    29461 aagaggtggt tggtctccaa gtggtccaag aagatatgcg aatggtggtt tgattacaaa
    29521 gcatcaactt gctgaagtgg gtgaaggaga taaacaggag atggttatcc ctttaactag
    29581 acgtaaacga gcaattcaat taactgaaca ggttatgcgc atcatcggta tggatggcaa
    29641 gccaaataac atcactgtaa ataatgatac ttcaacagtt gaaaaattgt tgaaacaaat
    29701 tgttatgtta agtgataaag gaaataaatt aacagatgca ttgattcaaa ctgtttcttc
    29761 tcaggataat aacttaggtt ctaatgatgc aattagaggt ttagaaaaaa tattgtcaaa
    29821 acaaagtggg catagagcaa atgcaaataa ttatatggga ggtttgacta attaatgcaa
    29881 tcttttgtaa aaatcataga tggttacaag gaagaagtaa taacagattt taatcagctt
    29941 atatttttag atgcaagggc tgaaagtcca aacaccaatg ataacagtgt aactattaac
    30001 ggagtagatg gtattttacc gggcgcaatt agttttgcgc ctttttcatt agtattaagg
    30061 tttggctatg atggtataga tgttatagat ttaaatttat ttgagcattg gtttagatct
    30121 gtgtttaatc gcagacatcc ttattatgtt attacttctc aaatgcctgg tgttaaatat
    30181 gcagtgaata cagctaatgt tacatctaat ttaaaagatg gttcttcaac tgaaattgaa
    30241 gtaagtttaa atgtttataa agggtattct gaatcagtta attggaccga tagcgagttc
    30301 ttattcgact ctaattggat gtttgaaaat ggaattcctc ttgatttcac acctaaatat
    30361 actcatacat caaatcaatt tactatttgg aacggttcta ctgatacgat aaatccacga
    30421 ttcaagcacg atttgaaaat attaattaat ttaaatgcga gtggaggatt tgaactggtt
    30481 aactatacaa caggtgatat ttttaagtac aacaaaagta tagataaaaa cactgatttt
    30541 gttttagatg gtgtgtatgc atatcgagat ataaatagag tgggaattga tacaaataga
    30601 ggcattataa cattagcgcc aggtaaaaat gaatttaaga ttaaaggaga cgtcagtgat
    30661 attaaaacta catttaagtt tccttttatt tataggtagg tgatttaatg gattatcatg
    30721 atcatttatc agtaatggat tttaatgaat tgatttgtga aaatttacta gatgtagatt
    30781 atggttcttt taaagaatat tatgaactga atgaagctag gtacatcacc tttacagttt
    30841 atagaactac tcataatagt tttgtttttg atttattgat ttgtgaaaac ttcataattt
    30901 atcatggtga aaaatataca attaagcaga cagcgccaaa ggttgaaggt gataaagttt
    30961 ttattgaagt tacggcatat cacataatgt atgaatttca aaatcactca gtggaatcaa
    31021 ataagcttga tgacgacagt agcgaaactg gtaaaacgcc agaatactct ttagatgagt
    31081 acttaagata tggatttgca aatcaaaaaa cttcggtcaa aatgacctat aaaataattg
    31141 gagattttaa gcgaaaagta ccgattgacg aattaggtaa caaaaacggc ttagaatact
    31201 gtaaagaagc ggtagacctg tttggctgta taatttaccc aaatgataca gagattggtt
    31261 tttattctcc tgaaacattt tatcaaagaa gcgagaaagt gattcgatat caatataata
    31321 ctgatactgt atctgcaact gtcagtacat tggaattaag aacagctata aaagtttttg
    31381 gaaaaaagta tacagctgag gaaaagaaaa attataatcc tattagaaca actgacatta
    31441 aatattcaaa tggttttata aaagaaggta cttatcgtac cgaaacaatt gggtctaaag
    31501 ctactattaa ctttgattgc aagtatggta atgaaacagt tagatttaca ataaaaaagg
    31561 gctctcaagg tggaatatat aagttgattt tagacggcaa gcaaattaag caaatttctt
    31621 gttttgctaa gtcggttcag tctgaaacaa tagatttaat aaaaaatatt gataaaggca
    31681 agcacgtttt agaaatgata tttttaggag aagaccccaa aaatagaatt gatatatctt
    31741 caaataaaaa agctaagcct tgtatgtatg ttggaactga aaaatcaaca gtcttaaatt
    31801 taattgctga taattcaggt cgcaatcaat acaaagcaat tgtcgactac gtcgcagata
    31861 gtgcaaagca gtttgggatt cgatatgcta atacgcaaac aaatgaagat atcgaaacac
    31921 aggataagct gttagaattt gcaaaaaagc aaataaatga tactcctaag actgaattag
    31981 atgttaatta tataggttat gaaaaaatag agccaagaga tagcgtattt tttgttcatg
    32041 aattaatggg atataacact gaattaaagg ttgttaaact tgataggtca catccatttg
    32101 taaacgcaat agatgaagtg tctttcagca atgaaataaa agatatggta caaattcaac
    32161 aagcacttaa cagacgagtt attgcacaag ataatagata taactatcaa gcaaatcgta
    32221 taaatcattt atacactagt actttgaatt ctcctttcga gacaatggat atagggagtg
    32281 tattaatata atggcaacag aagaagttaa aatcaaagcg ctacttgaaa acgataaaca
    32341 gtactttcca gctacacact ggaaagctat aaatgggata ccttatgcag gcagtagtga
    32401 tattgatgga ttgcctcaag acggtatcat ttcggtagat gataaaaata aattagataa
    32461 attaaaaata ggcgaagcag gaattattca aaatagcatt gtacagaaat ccccaaacgg
    32521 taaattgtgg aaaataacag ttgacgatag tgggaaactt ggtacagtgc tattttatta
    32581 gaaaggaagg tgcattatgg aaaatttgta tttaataaag gatttgggag ctttagcagg
    32641 tcgagattat agagctaaag aaattcaaaa cctgcaaaga atagagcaat ttgcgcttgg
    32701 cttgacaaca gagtttaagt tgcatcagaa agctaaaaca atgcaacact tcgctgagca
    32761 aatttattat aatggtagat cgcaagcagc agtaaacaaa tctttacaaa gtcaaattaa
    32821 cgcacttgtt gtggcaccac gtaataacag tgctaatgag attgttcaag ctcgagttaa
    32881 tgtaaacggc gaaacctttg acacattaaa agaacattta gacgattggg aaaccaaaac
    32941 tcaaattaat aaagaggaaa ctataagaga attaaataag accaaacaag aaattcttga
    33001 tatcgagtat cgttttgaac ctgataagca agagttttta tttgtgacag aacttgcacc
    33061 tcttacaaat gcagtaatgc aatccttctg gtttgataat agaacaggca tagtatacat
    33121 gacacaagct agaaataatg gctatatgct aagtcgttta agacctaatg gtcaatttat
    33181 agacagctca ttgattgtag gtgggggtca tggtacacat aacggttata gatatattga
    33241 tgatgagtta tggatttata gttttatctt aaatggtaat aatgagaata cattagttcg
    33301 tttcaagtat acgcctaatg tggaaattag ctatggcaag tatggtatgc aagatgtatt
    33361 tacaggacac ccagaaaaac cctacatcac ccctgtcata aatgaaaaag aaaataaaat
    33421 tctatacaga attgagagac ctagaagtca gtgggaactt gaaaactcaa tgaattatat
    33481 agagataaga agtttagacg atgttgataa aaatattgat aaagttttgc ataaaatcag
    33541 tatccctatg agactaacaa acgaaaccca accaatgcag ggtgtgactt ttgatgaaaa
    33601 atacttgtat tggtatacag gagacagtaa tccaaataat agaaactatt taacggcttt
    33661 cgatttagaa acaggagaag aagcgtatca ggttaatgct gactatggtg gaacactaga
    33721 ttcatttcct ggcgaatttg cggaagcaga aggtttgcaa atatactatg acaaagatag
    33781 tggtaaaaaa gctttgatgc taggtgttac tgtcggtggt gatggaaata gaacacatcg
    33841 tattttcatg attgggcaaa gaggtatttt agaaatactt cactcaagag gcgttccttt
    33901 tatcatgagt gacacaggtg gtagagttaa acctttacca atgaggcctg ataaacttaa
    33961 gaatcttggg atgttaacag agccaggtct ttactattta tacactgatc atacagttca
    34021 aatcgatgat ttcccattac caagagaatg gcgtgatgca ggttggttct tggaagttaa
    34081 gccaccacaa actggcggtg atgtaattca gatattgacg cgtaatagtt atgcaaggaa
    34141 tatgatgact tttgaaaggg tgctttctgg aagaactgga gacatttcgg actggaatta
    34201 tgtgcctaaa aatagtggta aatgggagag agtaccttca ttcatcacaa aaatgtcaga
    34261 tattaacata gtaggcatgt cgttttattt aactacggat gatacaaaac gttttacaga
    34321 ttttccaact gaacgtaaag gggtagctgg ttggaactta tatgtagaag cttcaaacac
    34381 aggtggcttt gttcataggc tagttcgtaa tagtgttaca gcatctgctg agatactatt
    34441 gaaaaattat gatagtaaaa caagttcagg gccatggact ttacacgaag ggagaattat
    34501 aagttaatga gtaatttaga gaaatctgta gctataaatt tagaaaacac agcgcattat
    34561 gaaaatattt caaatctaga tataactttt agaacaggag agagtgattc ttctgttctt
    34621 ctttttaata tcattaaaaa taatcaaccg ttattactga gtgaagaaaa tatcaaagca
    34681 cgaatagcga ttcgaggtaa aggagtaatg gtagttgctc cactagaaat attagatcca
    34741 tttaaaggta ttttaaaatt tcaattacct aatgatgtaa ttaaaagaga tggaagttat
    34801 caagctcaag tttcggttgc agaattaggt aattcagacg tggtagttgt agagagaact
    34861 atcacattta acgttgaaaa aagtttgttt agcaagattc cctctgaaac aaaactacac
    34921 tatattgttg agtttcaaga attagaaaaa actattatgg atcgcgcgaa agcaatggac
    34981 gaggctataa aaaatggtga ggattatgcg agtctgattg aaaaagctaa agaaaaaggt
    35041 ctatcagata ttcaaatagc aaaatcttca agtattgatg aattaaagca acttgctaat
    35101 agccgtatat ctgatttgga aaataaagcg caagcatatt caagaacatt cgatgagcaa
    35161 aagcgatata tggatgagaa acatgaagcc ttcaagcagt cagtgaatag tggtggttta
    35221 gtcacaagtg gttctacttc aaattggcaa aaagctaaga ttactaaaga tgatggtaag
    35281 ataatgcaga ttactggatt tgattttaat aatccagaac aaagaatagg tgattcaacc
    35341 caatttattt atgtttcgca agctataaat tatccaagag atgttagtac taacggtact
    35401 gtcgaatatt tagtagtaac ttcagattac aagcgtatga cttatcgacc gaacggtaca
    35461 aataaagtgt ttgttaaaag aaaagaagcg ggttcatggt ctgagtggtc agaattagct
    35521 attaatgatt acaatacacc ttttgaaact gttcaaagtg cccaatcaaa agctaatatg
    35581 gccgaaagta acgctaaatt atacgcagat gacaagttta ataaaaggta ttcggttatt
    35641 tttgatggaa cagcaaatgg tgtgggctct acattgtact taaatgagag tttagaccaa
    35701 tttattttat taatttttta tgggactttt ccaggtggtg actttacaga gtttggcagt
    35761 ccttttggag gaggaaagat ttcattgaat ccctcaaatc ttccagatgg tgatggaaat
    35821 ggtggaggtg tttatgagtt tggattaact aaatctagtc gtacatcttt aactatatca
    35881 aacgatgtct atttcgactt aggaagtcaa agaggctctg gtgcgaacgc aaatagaggg
    35941 acaattaaca aaattatagg agtgagaaaa taatgcaaat attagttaac aagcgtaatg
    36001 agataatttc atacgctatc attggcggct ttgaagaagg tattgatatt gaaaatttac
    36061 cagaaaattt ctctcaagtt tttagaccta aagcctttaa atattcaaat ggggaaatag
    36121 tttttaacga agattattca gaagaaaaag atgacttgca tcaacagatt gacagtgaag
    36181 aacaaaacac agtcgcttct gatgacatct tacgaaaaat ggttgctagt atgcagaaac
    36241 aagttgttca aagtacaaag ttatcgatgc aagttaataa gcaaaatgca ctaatggcaa
    36301 aacaacttgt gacacttaat aaaaaattag aagaggttaa aggagagact gaaaatgctt
    36361 aaattaattt caccaacatt cgaagatatt aaaacatggt atcaattgaa agaatatagt
    36421 aaagaagata tagcgtggta tgtagatatg gaagttatag ataaagagga atatgcaatt
    36481 attacaggag aaaagtatcc agaaaatcta gagtcatagg ttataatctt atggcttttt
    36541 aatttgaata aagtgggtgg tgtaatgttt ggatttacca aacgacacga acaagattgg
    36601 cgtttaacgc gattagaaga aaatgataag actatgtttg aaaaattcga cagaatagaa
    36661 gacagtctga gaacgcaaga aaaaatttat gacaagttag atagaaattt cgaagaacta
    36721 aggcgtgaca aagaagaaga tgaaaaaaat aaagagaaaa atgctaaaaa tattagagac
    36781 atcaagatgt ggattctagg attaataggg acgattctaa gtacatttgt tatagccttg
    36841 ttaaaaacta tttttggcat ttaaaggagg tgatcaccat gcttaaggga attttaggat
    36901 atagcttttg gtcgtgtttc tggtttggta agtgtaagta atatttaaga gtcagtgctt
    36961 cggcactggc tttttatttt ggataaaagg agcaaacaaa tggatgcaaa agtaataaca
    37021 agatacatcg tattgatctt agcattagta aatcaattct tagcgaataa aggtataagt
    37081 ccgataccag tagatgaaga aagtgtttca tcgattatct taacagttgt tgctttatat
    37141 actacatata aagataatcc aacatctcaa gaagggaaat gggcgaatca aaaattaaag
    37201 aaatataaag ctgaaagtaa atatagaaaa gcaacagggc aagcgccaat taaagaagta
    37261 atgacaccta cgaatatgaa cgacacaaat gatttagggt aggtgttgac caatgttgat
    37321 aacaaaaaac caagcagaaa aatggtttga taattcatta gggaagcagt tcaatcctga
    37381 tttgttttat ggatttcagt gttatgatta cgccaatatg ttctttatgt tagcgacagg
    37441 cgaaaggctg caaggtttat atgcttataa tatcccgttt gataataaag caaagattga
    37501 aaaatatggt caaataatta aaaactatga cagcttttta ccgcaaaagt tggatattgt
    37561 cgttttcccg tcaaagtatg gtggcggagc tggacacgtt gaaattgttg agagcgcaaa
    37621 tttaaacact ttcacatcat ttggtcaaaa ctggaacggt aaaggttgga ctaatggcgt
    37681 tgcgcaacct ggttggggtc ctgaaactgt gacaagacat gttcattatt atgacaatcc
    37741 aatgtatttt attaggttaa acttccctaa caacttaagc gttggcaata aagctaaagg
    37801 tattattaag caagcgacta caaaaaaaga ggcagtaatt aaacctaaaa aaattatgct
    37861 tgtagccggt catggttata acgatcctgg agcagtagga aacggaacaa acgaacgcga
    37921 ttttatacgt aaatatataa cgcctaatat cgctaagtat ttaagacatg caggacatga
    37981 agttgcatta tacggtggct caagtcaatc acaagatatg tatcaagata ctgcatacgg
    38041 tgttaatgta ggcaataaaa aagattatgg cttatattgg gttaaatcac aggggtatga
    38101 cattgttcta gaaatacatt tagacgcagc aggagaaagc gcaagtggtg ggcatgttat
    38161 tatctcaagt caattcaatg cagatactat tgataaaagt atacaagatg ttattaaaaa
    38221 taatttagga caaataagag gtgtaacacc tcgtaacgat ttactaaatg ttaatgtatc
    38281 agcagaaata aatatcaatt atcgcttatc tgaattaggt tttattacta ataaaaatga
    38341 tatggattgg attaagaaaa attacgactt gtactctaaa ctaatagctg gtgcgattca
    38401 tggtaagcca attggtggag tggtagctag tgaggtcaaa gcgccagtta aaaacgaaaa
    38461 gaatccgcca gtgccagcag gttatacact cgataagaat aatgtccctt ataaaaaaga
    38521 acaaggcaat tacacagtag ctaatgttaa aggtaataat gtaagagacg gttattcaac
    38581 taattcaaga attacagggg tattacccaa caacacaaca attacgtatg acggtgcata
    38641 ttgtattaat ggttatagat ggattactta tattgctaat agtggacaac gtcgctatat
    38701 tgcgaccgga gaggtagaca tagcaggcaa ccgaataagc agttttggta agtttagtgc
    38761 agtttgataa ttgtatatga tgaatcttag gcaggtactt cggtacttgc ctattattta
    38821 aaattaataa acagttaatt tttacatgaa tatattaaat tttaaaaaaa caaacgtttt
    38881 tagtatataa attattttgt gttcgtattg tgtgctatga ttaaaaagtt gttatggtca
    38941 actatatcgt ggt
//
LOCUS       DALAAR010000019        38530 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000019 DALAAR010000000
VERSION     DALAAR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.019"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            94..1431
                     /locus_tag="O2458_000990"
     CDS             94..1431
                     /locus_tag="O2458_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCZ3886873.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1433..2050
                     /locus_tag="O2458_000991"
     CDS             1433..2050
                     /locus_tag="O2458_000991"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCZ3886874.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2078..2407
                     /locus_tag="O2458_000992"
     CDS             2078..2407
                     /locus_tag="O2458_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCZ3886875.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2621..3547
                     /locus_tag="O2458_000993"
     CDS             2621..3547
                     /locus_tag="O2458_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCZ3886876.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3548..4351
                     /locus_tag="O2458_000994"
     CDS             3548..4351
                     /locus_tag="O2458_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCZ3886877.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4368..4715
                     /gene="yabA"
                     /locus_tag="O2458_000995"
     CDS             4368..4715
                     /gene="yabA"
                     /locus_tag="O2458_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCZ3886878.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4989..5714
                     /locus_tag="O2458_000996"
     CDS             4989..5714
                     /locus_tag="O2458_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCZ3886879.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5707..5955
                     /locus_tag="O2458_000997"
     CDS             5707..5955
                     /locus_tag="O2458_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCZ3886880.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5957..6796
                     /gene="rsmI"
                     /locus_tag="O2458_000998"
     CDS             5957..6796
                     /gene="rsmI"
                     /locus_tag="O2458_000998"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCZ3886881.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7081..9054
                     /gene="metG"
                     /locus_tag="O2458_000999"
     CDS             7081..9054
                     /gene="metG"
                     /locus_tag="O2458_000999"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCZ3886882.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9085..9858
                     /locus_tag="O2458_001000"
     CDS             9085..9858
                     /locus_tag="O2458_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCZ3886883.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10025..10561
                     /gene="rnmV"
                     /locus_tag="O2458_001001"
     CDS             10025..10561
                     /gene="rnmV"
                     /locus_tag="O2458_001001"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCZ3886884.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10572..11465
                     /gene="rsmA"
                     /locus_tag="O2458_001002"
     CDS             10572..11465
                     /gene="rsmA"
                     /locus_tag="O2458_001002"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCZ3886885.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11565..11828
                     /locus_tag="O2458_001003"
     CDS             11565..11828
                     /locus_tag="O2458_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCZ3886886.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12138..12986
                     /locus_tag="O2458_001004"
     CDS             12138..12986
                     /locus_tag="O2458_001004"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCZ3886887.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13000..13824
                     /gene="purR"
                     /locus_tag="O2458_001005"
     CDS             13000..13824
                     /gene="purR"
                     /locus_tag="O2458_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCZ3886888.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13841..14221
                     /locus_tag="O2458_001006"
     CDS             13841..14221
                     /locus_tag="O2458_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCZ3886889.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14293..14595
                     /gene="spoVG"
                     /locus_tag="O2458_001007"
     CDS             14293..14595
                     /gene="spoVG"
                     /locus_tag="O2458_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCZ3886890.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            14785..14916
                     /locus_tag="O2458_001008"
     CDS             14785..14916
                     /locus_tag="O2458_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886891.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14939..16291
                     /gene="glmU"
                     /locus_tag="O2458_001009"
     CDS             14939..16291
                     /gene="glmU"
                     /locus_tag="O2458_001009"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCZ3886892.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16438..17403
                     /locus_tag="O2458_001010"
     CDS             16438..17403
                     /locus_tag="O2458_001010"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCZ3886893.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17553..18206
                     /locus_tag="O2458_001011"
     CDS             17553..18206
                     /locus_tag="O2458_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCZ3886894.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18517..19089
                     /locus_tag="O2458_001012"
     CDS             18517..19089
                     /locus_tag="O2458_001012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCZ3886895.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19089..22595
                     /gene="mfd"
                     /locus_tag="O2458_001013"
     CDS             19089..22595
                     /gene="mfd"
                     /locus_tag="O2458_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCZ3886896.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKHPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22585..24111
                     /locus_tag="O2458_001014"
     CDS             22585..24111
                     /locus_tag="O2458_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ3886897.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24111..25304
                     /locus_tag="O2458_001015"
     CDS             24111..25304
                     /locus_tag="O2458_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ3886898.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25301..25564
                     /locus_tag="O2458_001016"
     CDS             25301..25564
                     /locus_tag="O2458_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCZ3886899.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25582..25974
                     /locus_tag="O2458_001017"
     CDS             25582..25974
                     /locus_tag="O2458_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCZ3886900.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26079..26480
                     /locus_tag="O2458_001018"
     CDS             26079..26480
                     /locus_tag="O2458_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCZ3886901.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26660..27955
                     /gene="tilS"
                     /locus_tag="O2458_001019"
     CDS             26660..27955
                     /gene="tilS"
                     /locus_tag="O2458_001019"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCZ3886902.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            27960..28499
                     /gene="hpt"
                     /locus_tag="O2458_001020"
     CDS             27960..28499
                     /gene="hpt"
                     /locus_tag="O2458_001020"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCZ3886903.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            28756..30849
                     /gene="ftsH"
                     /locus_tag="O2458_001021"
     CDS             28756..30849
                     /gene="ftsH"
                     /locus_tag="O2458_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCZ3886904.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            31078..31959
                     /gene="hslO"
                     /locus_tag="O2458_001022"
     CDS             31078..31959
                     /gene="hslO"
                     /locus_tag="O2458_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCZ3886905.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            32138..33070
                     /gene="cysK"
                     /locus_tag="O2458_001023"
     CDS             32138..33070
                     /gene="cysK"
                     /locus_tag="O2458_001023"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCZ3886906.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            33286..34089
                     /gene="folP"
                     /locus_tag="O2458_001024"
     CDS             33286..34089
                     /gene="folP"
                     /locus_tag="O2458_001024"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCZ3886907.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            34067..34432
                     /gene="folB"
                     /locus_tag="O2458_001025"
     CDS             34067..34432
                     /gene="folB"
                     /locus_tag="O2458_001025"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCZ3886908.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            34429..34905
                     /gene="folK"
                     /locus_tag="O2458_001026"
     CDS             34429..34905
                     /gene="folK"
                     /locus_tag="O2458_001026"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCZ3886909.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            35299..35391
                     /locus_tag="O2458_001027"
     CDS             35299..35391
                     /locus_tag="O2458_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886910.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            35444..36931
                     /gene="lysS"
                     /locus_tag="O2458_001028"
     CDS             35444..36931
                     /gene="lysS"
                     /locus_tag="O2458_001028"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCZ3886911.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            37489..37603
                     /gene="rrf"
                     /locus_tag="O2458_001029"
     rRNA            37489..37603
                     /gene="rrf"
                     /locus_tag="O2458_001029"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            37616..37691
                     /locus_tag="O2458_001030"
     tRNA            37616..37691
                     /locus_tag="O2458_001030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37649..37651,aa:Val,seq:tac)
     gene            37708..37783
                     /locus_tag="O2458_001031"
     tRNA            37708..37783
                     /locus_tag="O2458_001031"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37741..37743,aa:Thr,seq:tgt)
     gene            37790..37862
                     /locus_tag="O2458_001032"
     tRNA            37790..37862
                     /locus_tag="O2458_001032"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37823..37825,aa:Lys,seq:ttt)
     gene            37896..37970
                     /locus_tag="O2458_001033"
     tRNA            37896..37970
                     /locus_tag="O2458_001033"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37928..37930,aa:Gly,seq:gcc)
     gene            37978..38066
                     /locus_tag="O2458_001034"
     tRNA            37978..38066
                     /locus_tag="O2458_001034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38012..38014,aa:Leu,seq:taa)
     gene            38072..38148
                     /locus_tag="O2458_001035"
     tRNA            38072..38148
                     /locus_tag="O2458_001035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38106..38108,aa:Arg,seq:acg)
     gene            38169..38242
                     /locus_tag="O2458_001036"
     tRNA            38169..38242
                     /locus_tag="O2458_001036"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38203..38205,aa:Pro,seq:tgg)
     gene            38266..38341
                     /locus_tag="O2458_001037"
     tRNA            38266..38341
                     /locus_tag="O2458_001037"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38299..38301,aa:Ala,seq:tgc)
     gene            38464..>38530
                     /locus_tag="O2458_001038"
     rRNA            38464..>38530
                     /locus_tag="O2458_001038"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13804 a         5227 c         7800 g        11699 t
ORIGIN      
        1 aacactaact tttgtcaaag aagtgttact atataattaa tacttttgaa agtaactaat
       61 tccaaaacag tgtaaataaa ggaagcgtat atcatgaagc aacctatttt aaataaatta
      121 gaaagtttaa atcaagaaga agcgatttct ttgcatgttc cgggtcataa aaatatgact
      181 atcggtcatt tatctcaatt atcaatgaca atggataaaa ctgaaatacc tggattagat
      241 gatttacatc atcctgaaga agtcattttg gaaagtatga agcaggtgga gaaacattca
      301 gattatgatg cttatttctt agtgaatggc accacttcag gaatattatc tgtcatccag
      361 tctttttcac agaaaaaagg cgatatctta atggcaagaa atgtacataa atctgtgtta
      421 catgcgctcg atattagcca acaagaaggg cattttattg aaacgcatca aagtccgtta
      481 acgaatcatt ataataaagt taatttaagc cgtttgaata atgacggtca caaacttgct
      541 gtgttgactt atcctaacta ttacggtgaa acatttaatg tagaagaggt tatcaaattt
      601 ttgcaccaat taaatattcc tgtactcatt gacgaagcac acggcgcgca ctttggattg
      661 caaggatttc cagattctac attaaattat caagctgact atgttgttca atcttttcat
      721 aaaacgttac cagctttaac gatgggctcg gtactttata ttcataaaaa tgcaccttat
      781 agagaaacta ttatagaata tctaagctac ttccaaacat ctagtccttc gtatttgatt
      841 atggctagtt tagagtcagc tgccgagttc tataaaacat atgatagtac cgtgtttttt
      901 gataagagag cgcaattaat cgaatgtttg gagaagaagg gttttgaaat gcttcaagtt
      961 gatgatccgt tgaagttgct gataaaatat gaaggtttta caggtcatga tattcaaaat
     1021 tggtttatga atgcacatat ctatttagaa ttagcggacg actatcaagc attagcgata
     1081 ttgccgttat ggcatcatga tgatacgtat ttatttgatt cgcttttacg taaaattgaa
     1141 gatatgattt taccgaaaaa atcagtttct aaagttaaac aaacacaact tttaacaact
     1201 gaaggtaact ataaaccaaa acgctttgaa tatgttactt ggtgtgattt gaaaaaggca
     1261 aaaggtaaag ttctggcgcg acatattgtc ccgtatccgc cagggattcc tattattttc
     1321 aaaggagaaa caataactga aaatatgata gaattggtaa atgaatatct ggaaactgga
     1381 atgatagttg aaggaattaa aaataataaa attttagttg aggatgaata aaatgtcagc
     1441 ttttataact tttgagggcc cagaaggctc tggaaaaaca actgtaatta atgaagttta
     1501 ccatagatta gtaaaagatt atgatgtcat tatgactaga gaaccaggtg gtgttcctac
     1561 tggtgaagaa atacgtaaaa ttgtattaga aggcaatgat atggacatta gaactgaagc
     1621 aatgttattt gctgcatcta gaagagaaca tcttgtatta aaggtcatac cagctttaaa
     1681 agaaggtaag gttgtgttgt gtgatcgcta tatcgatagt tcattagctt atcaaggtta
     1741 tgctagaggg attggcgttg aagaagtaag agcattaaac gaatttgcaa taaatggatt
     1801 atatccagac ttgacgattt atttaaatgt tagtgctgaa gtaggtcgcg aacgtattat
     1861 taaaaattca agagatcaaa atagattaga tcaagaagat ttaaagtttc acgaaaaagt
     1921 aattgaaggt taccaagaaa tcattcataa tgaatcacaa cggttcaaaa gcgttaatgc
     1981 agatcaacct cttgaaaatg ttgttgaaga cacgtatcaa actatcatca aatatttaga
     2041 aaagatatga tataattgtt agaagaggtg ttataaaatg aaaatgatta tagcgatcgt
     2101 acaagatcaa gatagtcagg aacttgcaga tcaacttgtt aaaaataact ttagagcaac
     2161 aaaattggca acaacaggtg ggtttttaag agcgggtaat acaacattct tatgtggtgt
     2221 caatgatgac cgtgtagatg aaatattgtc tgtgattaat caaacgtgtg gtaatagaga
     2281 acagttggtt tcacctatta cacctatggg aggcagtgcg gattcgtaca ttccatatcc
     2341 agttgaagtt gaagttggcg gtgctactgt atttgttatg ccagttgatg cattccatca
     2401 attttaattc tataatacaa tcatcaatta gagatactta aaatagtgta ttaataagtt
     2461 attaacaatt ttgggttgct tgactgcgac tagttcagat gccaatagat ttgatttttg
     2521 tggttctaaa aataatcaca aatcatggtc gctattgttg cagtaattag ttgctctttg
     2581 gcaacctttt tatataaaag caaaagggag tttgtaatga atggatgaac agcaacaatt
     2641 gacgaatgca tatcattcaa ataaattatc gcatgcctat ttatttgaag gtgatgatgc
     2701 acaaacgatg aaacaagttg cgattaattt tgcaaagctt attttatgtc aaacagatag
     2761 tcaatgtgaa acaaaggtta gtacatataa tcatccagac tttatgtata tatcaacaac
     2821 tgagaatgca attaagaaag aacaagttga acaacttgtg cgtcatatga atcaacttcc
     2881 tatagaaagc acaaataaag tgtacatcat tgaagacttt gaaaagttaa ctgttcaagg
     2941 ggaaaacagt atcttgaaat ttcttgaaga accaccggac aatacgattg ctattttatt
     3001 gtctacaaaa cctgagcaaa ttttagacac aatccattca agatgtcagc atgtatattt
     3061 caagcctatt gataaagaaa agtttataaa tagattagtt gaacaaaaca tgtctaagcc
     3121 agtagctgaa atgattagta cttatactac gcaaatagat aatgcaatgg ctttaaatga
     3181 agaatttgat ttattagcat taaggaaatc agttatacgt tggtgtgaat tgttgcttac
     3241 taataagcca atggcactta taggtattat tgatttattg aaacaggcta aaaataaaaa
     3301 actgcaatct ttaactattg cagctgtgaa tggtttcttc gaagatatca tacatacaaa
     3361 ggtaaatgta gaggataaac aaatatatag tgatttaaaa aatgatattg atcaatatgc
     3421 gcaaaagttg tcgtttaatc aattaatttt gatgtttgat caactgacgg aagcacataa
     3481 gaaattgaat caaaatgtaa atccaacgct tgtatttgaa caaatcgtaa ttaagggtgt
     3541 gagttagatg ccaaatgtaa taggtgttca gtttcaaaaa gcgggaaaat tagaatatta
     3601 tacacctaat gatatacaag tagatataga agactgggta gttgtcgaat ctaaaagagg
     3661 catagagata ggtattgtta aaaatccatt aatggatatt gctgaagagg atgttgtgtt
     3721 acctcttaaa aatattattc gcattgctga tgacaaagat attgataaat ttaattgtaa
     3781 tgaacgagat gctgaaaatg cattaatact atgtaaagac attgtaagag aacaaggttt
     3841 ggacatgcgt ttagtcaatt gcgaatatac attagataaa tcgaaagtta tttttaattt
     3901 tacggcggat gatcgtattg attttagaaa attagtaaaa atattagcgc aacatttaaa
     3961 aacacgtatc gagttgagac aaattggtgt aagggatgaa gccaaattgc ttggcggtat
     4021 cggaccttgt ggtaggtcgt tatgttgttc tacattttta ggggattttg aaccagtatc
     4081 gattaagatg gctaaggatc aaaatttatc attaaatcca actaaaattt ctggtgcatg
     4141 tggtcgtttg atgtgttgtt taaaatatga aaatgactat tatgaggaag tacgtgcaca
     4201 attacctgat attggtgaag caattgaaac gcctgatggt aacgggaaag tagttgcttt
     4261 aaatatatta gacatttcta tgcaggtgaa gcttgaggga catgaacagc cacttgaata
     4321 taaattagaa gaaatagaaa ctatgcatta aggaggcatt attacatttg gatcgcaatg
     4381 aaatatttga aaaaataatg cgtttagaaa tgaatgtcaa tcaactttca aaggaaactt
     4441 cagaattaaa ggcacttgca gttgaattag tagaagaaaa tgtagcgctt caacttgaaa
     4501 atgataattt gaaaaaggtg ttgggcaatg atgaaccaac tactattgat actgcgaatt
     4561 caaaaccagc aaaagctgtg aaaaagccat taccaagtaa agataatttg gctatattgt
     4621 atggagaagg atttcatatt tgtaaaggcg aattatttgg aaaacatcga catggtgaag
     4681 attgtctgtt ctgtttagaa gttttaagtg attaatcaag cacactcaaa tagtgttata
     4741 attataaatg aatatggttt ggataagtct gagacaatgc atgtttcagg ctttaattgt
     4801 gtataaagtt ttggtgattg cataagagat ggcggtacta aatgttatta ttaagtgtgc
     4861 acgcagtatc attagttata aaatgtagct gttaaaagtc aaaaatacat cgaatgtagt
     4921 taggcatata atataaaaag agttttcaat tactcaatag aaaaaggttg tcttcatagg
     4981 agttaaaaat gttaaaagag aatgaacgat ttgatcaact aatcaaagaa gattttagta
     5041 ttattcaaaa tgatgatgtt ttttcatttt caacggatgc tttgttgtta gggcatttta
     5101 caaaacctag aacaaaagat attgtgttgg acttatgttc aggcaatggg gtgataccct
     5161 tgttattgtt tgcgaaacat ccacgacata tagaaggtgt tgagattcaa aaaacacttg
     5221 tcgatatggc gcgacgcaca tttcaattca atgatgttga tgaatattta acaatgcatc
     5281 acatggattt gaaaaacgtt actaaagtat ttaaaccttc acaatatact ttagtaacgt
     5341 gtaatccgcc ttattttaaa gagaatcagc aacaccaaca tcaaaaagaa gcacataaga
     5401 tagcgagaca tgagattatg tgtacacttg aagattgcat gattgcagcc cgtcatttat
     5461 taaaagaagg tggcaggcta aacatggtac atcgtgcaga gagactaatg gatgtcttgt
     5521 ttgaaatgag aaaagtgaat attgaaccta agaaagtcgt ttttatatat agtaaagtag
     5581 ggaaatcagc acaaacgata gtagtagaag gtcgaaaagg tggaaatcaa ggtttagaaa
     5641 tcatgccccc attttatatt tataatgaag atggtaatta tagcgaagaa atgaaggaag
     5701 tatattatgg atagtcattt tgtatatatt gtaaaatgta gtgatggaag tttatataca
     5761 ggatacgcta aagacgttaa tgcacgtgtt gaaaaacata accgaggtca aggagccaaa
     5821 tatacgaaag taagacgtcc ggtgcattta gtttatcaag aaatgtatga gacaaagtct
     5881 gaagcattga agcgtgaata tgaaattaaa acttatacca gacaaaagaa attgcgatta
     5941 attaaggagc gatagtatgg ctgtattata tttagtgggc acaccaattg gtaatttagc
     6001 agatattact tatagagcag ttgatgtatt gaaacgtgtt gatatgattg cttgtgaaga
     6061 cactagagta actagtaaac tgtgtaatca ttatgatatt ccaactccat taaagtcata
     6121 tcacgaacat aacaaggata agcagactgc ttttatcatt gaacagttag aattaggtct
     6181 tgacgttgcg ctcgtatctg atgctggatt gcccttaatt agtgatcctg gatacgaatt
     6241 agtagtggca gccagagaag ctaatattaa agtagagacc gtgcctggac ctaatgctgg
     6301 gctgacggct ttgatggcta gtggattacc ttcatatgta tatacatttt taggattttt
     6361 gccacgaaaa gagaaagaaa aaagtgctgt attagagcaa cgtatgcatg aaaatagcac
     6421 attaattata tacgaatcac cgcatcgtgt gacagataca ttaaaaacaa ttgcaaagat
     6481 agatgcaaca cgacaagtat cactagggcg tgaattaact aagaagttcg aacaaattgt
     6541 aactgatgat gtaacacaat tacaagcatt gattcagcaa ggcgatgtac cattgaaagg
     6601 cgaattcgtt atcttaattg aaggtgctaa agcgaacaat gagatatcgt ggtttgatga
     6661 tttatctatc aatgagcatg ttgatcatta tattcaaact tcacagatga aaccaaaaca
     6721 agctattaaa aaagttgctg aagaacgaca acttaaaacg aatgaagtat ataatattta
     6781 tcatcaaata agttaatcac tttatcgatt atatgaaatt ttaaacgatt ttataaacgc
     6841 aagctgtaat tttaaatggt aagttatcat tttgcattga tactgataaa atgatgttga
     6901 ctatgataaa aaaatgatga catcgacgtt ttttaatgta aaataaatac attgaaagta
     6961 ataaatacct taacattgaa taagatgaaa atgagatgac gagataaatg ttcgcgtccg
     7021 ttgaaatgca tagaaatctt agatattatt tgaagtgaga cattacgagg aggaacagtt
     7081 atggctaaag aaacatttta tataacaacc ccaatatact atcctagtgg gaatttacat
     7141 ataggacatg catattctac agtggctgga gatgttattg caagatataa gagaatgcaa
     7201 ggatatgatg ttcgctattt gactggaacg gatgaacacg gtcaaaaaat tcaagaaaaa
     7261 gctcaaaaat ctggtaagac agaaattgaa tatttggatg agatgattgc tggaattaaa
     7321 caattgtggg ctaagcttga aatttcaaat gatgatttta tcagaacaac tgaagaacgt
     7381 cataaacatg tcgttgagca agtgtttgaa cgtttattaa agcaaggtga tatctattta
     7441 ggtgaatatg aaggttggta ttctgttccg gatgaaacat actatacaga gtcacaatta
     7501 gtagacccac aatacgaaaa cggtaaaatt attggtggca aaagtccaga ttctggacac
     7561 gaagttgaac tagttaaaga agaaagttat ttctttaata ttagtaaata tacagaccgt
     7621 ttattagagt tctatgacca aaatccagat tttatacaac caccatcaag aaaaaatgaa
     7681 atgattaaca acttcattaa accaggactt gctgatttag ctgtttctcg tacatcattt
     7741 aactggggtg tccatgttcc gtctaatcca aaacatgttg tttatgtttg gattgatgcg
     7801 ttagttaact atatttcagc attaggctat ttatcagatg atgagtcact atttaacaaa
     7861 tactggccag cagatattca tttaatggct aaggaaattg tgcgattcca ctcaattatt
     7921 tggcctattt tattgatggc attagactta ccgttaccta aaaaagtctt tgcacatggt
     7981 tggattttga tgaaagatgg aaaaatgagt aaatctaaag gtaatgtcgt agaccctaat
     8041 attttaattg atcgctatgg tttagatgct acacgttatt atctaatgcg tgaattacca
     8101 tttggttcag atggcgtatt tacacctgaa gcatttgttg agcgtacaaa tttcgatcta
     8161 gcaaatgact taggtaactt agtaaaccgt acgatttcta tggttaataa gtactttgat
     8221 ggcgaattac cagcgtatca aggtccactt catgaattag atgaagaaat ggaagctatg
     8281 gctttagaaa cagtgaaaag ctacactgaa agcatggaaa gtttgcaatt ttctgtggca
     8341 ttatctacgg tatggaagtt tattagtaga acgaataagt atattgacga aacaacgcct
     8401 tgggtattag ctaaggacga tagccaaaaa gatatgttag gcaatgtaat ggctcactta
     8461 gttgaaaata ttcgttatgc agctgtatta ttacgtccat tcttaacaca tgcgccgaaa
     8521 gagatttttg aacaattgaa cattaacaat cctcaattta tggaatttag tagtttagag
     8581 caatatggtg tgcttaatga gtcaattatg gttactgggc aacctaaacc tattttccca
     8641 agattggata gcgaagcgga aattgcatat atcaaagaat caatgcaacc gcctgctact
     8701 aaagaggaaa aagaagagat tcctagcaaa cctcaaattg atattaaaga ctttgataaa
     8761 gttgaaatta aggcagcaac gattattgat gctgaacatg ttaagaagtc agataagctt
     8821 ttaaaaattc aagtagactt agattctgaa caaagacaaa ttgtatcagg aattgccaaa
     8881 ttctatacac cagatgatat tattggtaaa aaagtagcag ttgttactaa cctgaaacca
     8941 gctaaattaa tgggacaaaa atctgaaggt atgatattat ctgctgaaaa agatggtgta
     9001 ttaaccttag taagtttacc aagtgcaatt ccaaatggtg cagtgattaa ataactgtat
     9061 ttttaaaaat taggagagat aattatgtta atcgatacac atgtccattt aaatgatgag
     9121 caatacgatg atgatttgag tgaagtgatt acacgtgcta gagaagcagg tgttgatcgt
     9181 atgtttgtag ttggttttaa caaatcgaca attgaacgcg cgatgaaatt aatcgatgag
     9241 tatgattttt tatatggcat tatcggttgg catccagttg acgcaattga ttttacagaa
     9301 gaacacttgg aatggattga atctttagct cagcatccaa aagtgattgg tattggtgaa
     9361 atgggattag attatcactg ggataaatct cctgcagatg ttcaaaagga agtttttaga
     9421 aagcaaattg ctttagctaa gcgtttgaag ttaccaatta tcattcataa ccgtgaagca
     9481 actcaagact gtatcgatat cttattggag gagcatgctg aagaggtagg cgggattatg
     9541 catagcttta gtggttctcc agaaattgca gatattgtaa ctaataagct gaatttttat
     9601 atttcattag gtggacctgt gacatttaaa aatgctaaac agcctaaaga agttgctaag
     9661 catgtgtcaa tggagcgttt gctagttgaa accgatgcac cgtatctttc gccacatccg
     9721 tatagaggga agcgaaatga accggcgaga gtaactttag tagctgaaca aattgctgaa
     9781 ttaaaaggct tatcttatga agaagtgtgc gaacaaacaa ctaaaaatgc agagaaattg
     9841 tttaatttaa attcataaag ttaaaagtga gaaagatcac cgccataaat gtaaacgatg
     9901 ctatattcgt ttaatatgct atggttcttt ctcacttttt taaattaaaa tatcgtgcat
     9961 gtggaatacg tgcgatagag atggttagag ctttgaaatt aagaattgta ggaaggcgtt
    10021 ttaaatgaaa atcaatgagt ttatagttgt agaaggacga gatgatactg agcgtgttaa
    10081 acgagctgtt gaatgtgata cgattgaaac gaatggtagt gccatcaacg aacaaacttt
    10141 agaagtaatt agaaatgctc aacaaagtcg aggcgttatt gtattaacag atccagattt
    10201 cccaggagat aaaattagaa gtacaattac tgaacatgtc aaaggtgtta aacatgcgta
    10261 tattgataga gaaaaagcta aaaataaaaa agggaaaatt ggtgttgaac atgccgaatt
    10321 aattgatatt aaagaagcgt taatgcatgt tagttcaccc tttgatgaag cttatgaatc
    10381 aattgataaa tctgtgctaa tagagttggg gttaattgtt gggaaagatg caaggcgccg
    10441 tagagaaatt ttaagtagaa aattgcgaat cggccattcc aatggtaagc agttattgaa
    10501 aaagttaaat gcatttggtt ataccgaagc ggatgtaagg caagctttag aagatgaatg
    10561 aggaagtgaa aatgttggat aataaagata ttgcaacacc atcaagaacg cgagcgttgt
    10621 tagataaata tggctttaat tttaaaaaaa gtttaggaca gaactttttg atagatgtga
    10681 atatcattaa taatatcatt gatgcaagtg atattgatgc acaaactggg gtgattgaaa
    10741 ttggtccagg catggggtca ttgacagaac aattggccag acatgctaaa agagtattgg
    10801 catttgaaat tgatcaacgt ttaatacctg tattaaatga tacactatca ccttatgata
    10861 atgtgacggt gattaatgaa gatattttaa aagcgaatat taaagaagct gttgaaaatc
    10921 atttacaaga ttgtgaaaaa ataatggttg ttgcaaacct gccgtactat attacgacgc
    10981 caattttatt aaatttgatg caacaagata taccaattga tggctacgtg gtgatgatgc
    11041 aaaaagaagt gggcgaacgc ttaaatgctg aagtaggttc aaaagcatat ggttcgttat
    11101 caattgtcgt acaatactat acagagacta gtaaagtatt aacggtacct aaatctgtat
    11161 ttatgccacc acctaatgtt gattcaatag ttgtaaaact gatgcagaga actgaaccgt
    11221 tagtaacagt agataacgag gaagcattct ttaagttagc aaaagcagca tttgcacaaa
    11281 gaagaaagac aattaacaat aactatcaaa attattttaa agatggtaaa caacacaaag
    11341 aagtgatttt acaatggttg gaacaagcag gtattgatcc aagacgtcgc ggtgaaacgc
    11401 tatctattca agattttgct aaattgtatg aagaaaagaa aaaattccct caattagaaa
    11461 attaaatgat tgacaaagca aagcactatt gttaaaattt aaattttgtt tgacgaaaac
    11521 gttgcaaata tggtattatg taacttgtag cgaggtggag caatatgcca aaatcaattt
    11581 tggacatcaa aaattctatt gattgtcatg taggaaatcg tattgtactg aaagccaatg
    11641 gaggccgtaa gaaaacaata aaacgttctg gaattttaaa agaaacatat ccgtcagttt
    11701 tcattgttga gttagatcaa gacaaacaca actttgagag agtatcttat acatacactg
    11761 atgtgttaac tgaaaatgtt caagtttcat ttgaagagga taatcatcac gaatcaattg
    11821 cacactaaat aagacatata gagatgttag acgtttctta gtataagaag taaatattat
    11881 gataattatt tgagtgttgg gcattatgtt caatactctt tttatttaca aaatgtttaa
    11941 cactgatgtt tcgcttatag atttttcagt aaatggatac ttgtatttat aaacacaaat
    12001 acaagtaaat actaagtaat tagatggaga aaattacttt tttattaaaa aaacactaaa
    12061 aaacaaatta aaatgtcaaa tattaattct ctttatgtta aaatcatcat attaagataa
    12121 cgaaaagagg gcggaaaatg atatatgaaa cggcaccagc caaaattaat tttacgctcg
    12181 atacactttt taaaagaaat gatggctatc atgagattga aatgataatg acaacagttg
    12241 atttaaatga tcgtttaact tttcataaaa gaaaagatcg aaagatagtt gttgagattg
    12301 aacataatta tgtgccttct aatcataaaa atctcgcata tcgtgcagcg caactattta
    12361 ttgagcaata tcaactaaag caaggtgtaa caatttctat cgataaagaa atacctgttt
    12421 ctgctggctt agctggaggt tcggctgatg cagcagcaac gttaagagga ttgaatcgac
    12481 tttttgatat aggggcgagt ttggaagaat tggctctact aggcagtaaa atcgggacag
    12541 atattccgtt ttgtatttat aataaaactg cactatgtac tggaagagga gagaaaatcg
    12601 agtttttaaa taaaccacct tcagcttggg tgattcttgc taaaccaaac ttaggcatat
    12661 catcaccaga tatatttaag ttgattaatt tagataagcg ttacgacgta catacgaaaa
    12721 tgtgttatga ggccttagaa aatcgagatt atcaacaatt atgtcaaagt ttgtctaatc
    12781 gattagagcc aatttctgtt tcaaaacacc cacaaatcga taaattaaaa aataatatgt
    12841 tgaaaagtgg tgcagatggt gcgttaatga gtggaagcgg acctactgtg tatgggctag
    12901 cacgaaaaga aagccaagca aaaaatattt ataatgcagt taacggttgt tgtaatgaag
    12961 tgtacttagt tagactatta ggatagaagg gttgaaaaga tgagatataa acgaagcgag
    13021 agaattgttt ttatgacgca atatttgatg aaccatccga ataaattgat tccattaact
    13081 ttttttgtga aaaaatttaa acaggcgaag tcttcaataa gtgaagatgt ccaaattata
    13141 aaaaatacat tccaaaaaga aaagttaggt acagtaatta ctactgctgg cgcaagtggt
    13201 ggtgttacgt ataaaccaat gatgagtaaa gaagaggcga ctgaagttgt taatgaggtc
    13261 attactctat tagaagagaa agaacgtttg ttacctggcg gatatttatt tttatcagat
    13321 ttggtaggta atccatcgct actaaacaaa gttggtaagt taattgccag tatttacatg
    13381 gaagaaaaat tagatgctgt tgttaccatt gcgacaaaag gtatttcatt ggcaaatgcg
    13441 gttgctaata ttttaaattt accagtagta gtgattagaa aagacaacaa ggtgactgaa
    13501 ggttctacag tttcaattaa ttacgtttca ggatcttcaa gaaaaataga aacaatggta
    13561 ctttcgaaga gaactttagc agaaaattca aatgttttag ttgtcgatga ttttatgagg
    13621 gctggtggct ctattaatgg tgttatgaat ttaatgaatg agtttaaagc ccatgtaaaa
    13681 ggggtatcag tacttgtaga atcaaaagaa gttaaacaaa gattgattga agattatact
    13741 tccttagtga aattatctga tgtagatgaa tataatcaag agtttaacgt agaacctggc
    13801 aacagtttat ctaagttttc ataaaaggag ttttagtatt atgaaaatca ttaacacaac
    13861 aagattaccg gaagcacttg gaccatattc gcatgcaaca gttgtgaatg gtatggttta
    13921 tacttctggt cagattccat tgaatattga tggacatatc gtaagcgctg atgttcaagc
    13981 acagacaaaa caagttttag aaaatttaaa ggttgttttg gaagaagcag gatctgattt
    14041 gaattctgtt gcgaaagcga ccattttcat taaagatatg aatgatttcc aaaaaataaa
    14101 tgaagtgtat ggtcaatatt ttaatgaaca caagccagcg cgtagttgtg tagaggttgc
    14161 gcgtttgcca aaagatgtga aagtagaaat tgaattagta agtaaaatta aggaattata
    14221 attttcgatt aatatgttta atcaagcttc taaataaaac agagagatat atactatagg
    14281 ggggctcact acatgaaagt gacagatgta agacttagaa aaatacaaac agatggacga
    14341 atgaaagcac tcgtttccat tacattagat gaagctttcg taattcatga tttacgtgta
    14401 attgaaggaa actctggctt gttcgttgca atgccaagta aacgtacacc agatggtgaa
    14461 ttccgcgaca tcgcgcatcc tattaattca gatatgagac aagaaattca agatgcagtg
    14521 atgaaagtat atgatgaaac agatgaagta gtaccagata aaaacgctac atcagaagat
    14581 tcagaagaag cttaatcaat tttatattta gcgatgtaat acatttgcaa taagttgatt
    14641 tgatactgtc gataaagcat aaagctttgt cggcagtttt tttagtttgt attaatgttt
    14701 ttttattttt aatgaaaggc taataaatat atacgttaac agattatgat gatatgaaaa
    14761 ttattgattc attgctgata aaaattgcgt ttgaatgatg ctcgtatttt tgaagtaaga
    14821 aaaaagttgt ttttaaaatt acaacgaatt aaaaacaatg ccttttatat gttgaaagag
    14881 tattgcagat taaattataa taatgacgaa gtgtaaaatt taatgggggt taatgttcat
    14941 gcgaagacac gcgataattt tggcagcagg taaaggcaca agaatgaaat ctaaaaagta
    15001 taaagtgcta cacgaggttg ctgggaaacc tatggtcgaa catgtattgg aaagtgtgaa
    15061 aggctctggt gtcgatcaag ttgtaaccat cgtaggacat ggtgctgaaa gtgtaaaagg
    15121 acatttaggc gagcgttctt tatacagttt tcaagaggaa caactcggta ctgcgcatgc
    15181 agtgcaaatg gcgaaatcac acttagaaga caaggaaggt acgacaatcg ttgtatgtgg
    15241 tgacacaccg ctcatcacaa aggaaacatt agaaacattg attgcgcatc acgaggatgc
    15301 taatgctcaa gcaactgtat tatctgcatc gattcaacaa ccatatggat acggaagaat
    15361 cgttcgaaat gcgtcaggtc gtttagaacg catagttgaa gagaaagatg caacgcaagc
    15421 tgaaaaggat attaatgaaa ttagttcagg tatttttgcg tttaataata aaacgttgtt
    15481 tgaaaaatta acacaagtga aaaatgataa tgcgcaaggt gaatattacc tccctgatgt
    15541 attgtcgtta attttaaatg atggcggcat cgtagaagtc tatcgtacca atgatgttga
    15601 agaaatcatg ggtgtaaatg atcgtgtaat gcttagtcag gctgagaagg cgatgcaacg
    15661 tcgtacgaat cattatcaca tgctaaatgg tgtgacaatc atcgatcctg acagcactta
    15721 tattggtcca gacgttacaa ttggtagtga tacagtcatt gaaccaggcg tacgaattaa
    15781 tggtcgtaca gaaattggcg aagatgttgt tattggtcag tactctgaaa ttaacaatag
    15841 tacgattgaa aatggtgcat gtattcaaca gtctgttgtt aatgatgcta gcgtaggagc
    15901 gaatactaag gtcggaccgt ttgcgcaatt gagaccaggc gcgcaattag gtgcagatgt
    15961 taaggttgga aattttgtag aaattaaaaa agcagatctt aaagatggtg ccaaggtttc
    16021 acatttaagt tatattggcg atgctgtaat tggcgaacgt actaatattg gttgcggaac
    16081 gattacagtt aactatgatg gtgaaaataa atttaaaact atcgtcggca aagattcatt
    16141 tgtaggttgc aatgttaatt tagtagcacc tgtaacaatt ggtgatgatg tattggtggc
    16201 agctggttcc acaatcacag atgacgtacc aaatgacagt ttagctgtgg caagagcaag
    16261 acaaacaaca aaagaaggat ataggaaata atcatttacg tatttaaaat ggctaggata
    16321 aaaggataat cctatgtaat attaatgtaa tctttatgat ttaatgattc gcatagtaat
    16381 ggagttacat tttatatata atagtaattg cgtaagtaaa taattggagg actataaatg
    16441 ttaaataatg aatataagaa ttcgtcatta aagatttttt cattgaaagg aaacgaagca
    16501 ttagcgcaag aagttgctga ccaagtagga attgaactag gtaaatgttc agttaaacgt
    16561 tttagtgatg gagaaattca aattaatatc gaagagagta ttcgtggttg tgacgtattt
    16621 attattcaac caacatcata tcctgtgaat ctacatttaa tggaattatt aattatgatt
    16681 gatgcttgta aacgtgcttc tgcagcaaca atcaatattg tagtgccata ttatggatat
    16741 gcaagacaag atagaaaagc ccgtagccgt gagccaatca ctgctaaatt agttgcaaac
    16801 ttaatcgaaa cagctggcgc aactcgtatg attgcgttag acttacatgc accacaaatt
    16861 caaggatttt ttgatattcc aattgaccac ttaatgggtg tgccaattct tgctaaacat
    16921 ttcaaagatg atccgaatat taacccagaa gaatgtgtcg ttgtttcacc agaccatggc
    16981 ggcgttacac gtgcacgtaa attagctgac attttaaaaa ctccaattgc aattatagat
    17041 aaacgtcgtc ctagaccaaa tgttgctgaa gtgatgaaca ttgttggtga gattgaagga
    17101 cgtacggcaa ttattattga cgatattatt gatacagcag gtacaatcac tttagctgca
    17161 caagcattaa aagataaagg tgctaaagaa gtatatgctt gttgtacaca ccctgtttta
    17221 tcaggaccgg ctaaagaacg tatcgaaaat tctgctataa aagaattaat cgtaacaaac
    17281 tcaattcatt tagatgaaga tcgcaaacca tctaacacta aagaattatc tgttgctggt
    17341 ttaatcgcac aagctatcat tcgtgtatac gaaagagaat cagttagcgt attatttgac
    17401 taatatttaa aaggcgtttg acgaacatat tccaaacgtg tataatagtt tcgttcgtga
    17461 ttatacgaat aaataaacac ttgcaagcaa cgatgatgtt gatgggtaag tgaggtgctc
    17521 gttttgagca aaaatgaaag gtggaaatga gaatggcttc attaaagtca atcatccgtc
    17581 aaggtaaaca aacacgttca gatcttaaac aattaagaaa atctggtaaa gtaccagcag
    17641 tagtatacgg ttacggtact aaaaacgtgt cagttaaagt tgatgaagta gaattcatca
    17701 aagttatccg tgaagtaggt cgtaacggtg ttatcgaatt aggcgttggt tctaaaacta
    17761 tcaaagttat ggttgcagac taccaattcg atccacttaa aaaccaaatt actcacattg
    17821 acttcttagc aatcaatatg agtgaagaac gtactgttga agtaccagtt caattagttg
    17881 gtgaagcagt aggcgctaaa gaaggcggcg tagttgaaca accattattc aacttagaag
    17941 taactgctac tccagacaat attccagaag caatcgaagt agacattact gaattaaaca
    18001 ttaacgacag cttaactgtt gctgatgtta aagtaactgg cgacttcaaa atcgaaaacg
    18061 attcagctga atcagtagta acagtagttg ctccaactga agaaccaact gaagaagaaa
    18121 tcgaagctat ggaaggcgaa caacaaactg aagaaccaga agttgttggc gaaagcaaag
    18181 aagacgaaga aaaaactgaa gagtaatttt aatctgttac attaaagttt ttatactttg
    18241 tttaacaagc actgtgctta ttttaatata agcatggtgc tttttgtgtt attataaagc
    18301 ttaattaaac tttattactt tgtactaaag tttaattaat tttagtgagt aaaagacatt
    18361 aaactcaaca atgatacatc ataaaaattt taatgtactc gattttaaaa tacatactta
    18421 ctaagctaaa gaataatgat aattgatggc aatggcggaa aatggatgtt gtcattataa
    18481 taataaatga aacaattatg ttggaggtaa acacgcatga aatgtattgt aggtctaggt
    18541 aatataggta aacgttttga acttacaaga cataatatcg gctttgaagt cgttgattat
    18601 attttagaga aaaataattt ttcattagat aaacaaaagt ttaaaggtgc atatacaatt
    18661 gaacgaatga acggcgataa agtgttattt atcgaaccaa tgacaatgat gaatttgtca
    18721 ggtgaagcag ttgcaccgat tatggattat tacaatgtta atccagaaga tttaattgtc
    18781 ttatatgatg atttagattt agaacaagga caagttcgct taagacaaaa aggaagtgcg
    18841 ggcggtcaca atggtatgaa atcaattatt aaaatgcttg gtacagacca atttaaacgt
    18901 attcgtattg gtgtgggaag accaacgaat ggtatgacgg tacctgatta tgttttacaa
    18961 cgcttttcaa atgatgaaat ggtaacgatg gaaaaagtta tcgaacacgc agcacgcgca
    19021 attgaaaagt ttgttgaaac atcacgattt gaccatgtta tgaatgaatt taatggtgaa
    19081 gtgaaataat gacaatattg acaacgctta taaaagaaga taatcatttt caagacctta
    19141 atcaggtatt tggacaagca aacacactag taactggtct ttccccgtca gctaaagtga
    19201 cgatgattgc tgaaaaatat gcacaaagta atcaacagtt attattaatt accaataatt
    19261 tataccaagc agataaatta gaaacagatt tacttcaatt tatagatgct gaagaattgt
    19321 ataagcatcc tgtgcaagat attatgaccg aagagttttc aacacaaagc cctcaactga
    19381 tgagtgaacg tattagaact ttaactgcgt tagctcaagg taagaaaggg ttatttatcg
    19441 ttcctttaaa tggtttgaaa aagtggttaa ctcctgttga aatgtggcaa aatcaccaaa
    19501 tgacattgcg tgttggtgag gatatcgatg tggaccaatt tcttaacaaa ttagttaata
    19561 tggggtacaa acgggaatcc gtggtatcgc atattggtga attctcattg cgaggaggta
    19621 ttatcgatat ctttccgcta attggggaac caatcagaat tgagctattt gataccgaaa
    19681 ttgattctat tcgggatttt gatgttgaaa cgcagcgttc caaagataat gttgaagaag
    19741 tcgatatcac aactgcaagt gattatatca ttactgaaga agtgatcagc catcttaaag
    19801 aagagttaaa aactgcatat gaaaatacaa gacccaaaat agataaatca gtgcgcaatg
    19861 atttgaaaga aacgtatgaa agctttaaat tattcgaaag tacatacttt gatcatcaaa
    19921 tactacgtcg cttagtagcg tttatgtatg aaacaccttc gacaattatt gagtatttcc
    19981 aaaaagatgc aatcattgca gttgatgaat ttaatcgtat taaagaaact gaagaaagtt
    20041 taacagtaga gtctgattcg tttattagca atattattga aagtggtaat ggatttatag
    20101 gacaaagttt tataaaatat gatgattttg aaacattgat tgaaggctat cctgtcactt
    20161 atttttcatt attcgctaca acaatgccga taaaactaaa tcatattatt aaattttcat
    20221 gtaaacctgt ccaacaattt tatgggcaat atgacattat gcgttctgaa tttcaacgat
    20281 atgttaatca aaactatcat atcgtggttt tggtcgaaac cgaaactaaa gttgaacgta
    20341 tgcaagcgat gttaagtgaa atgcatattc catcaataac aaaattgcat cgctcaatgt
    20401 catcggggca agcagtgatt attgaaggca gtttatctga aggatttgaa ctacctgata
    20461 tgggattagt tgtcattact gagcgtgagc tttttaaatc aaaacagaaa aagcaacgaa
    20521 aacgtacgaa agctatctca aatgctgaaa aaattaagtc ttaccaagat ttaaatgtgg
    20581 gagattatat tgttcatgtg catcatggtg ttggtagata tttaggtgtt gagacgctcg
    20641 aagtggggca aacgcatcgt gattatatta aattgcaata taaaggtacg gatcaactat
    20701 ttgttccagt agatcaaatg gatcaagttc aaaaatatgt agcttcggaa gataagacgc
    20761 caaaattaaa taaactcggt ggcagtgaat ggaaaaaaac aaaagctaaa gttcaacaaa
    20821 gtgttgaaga tattgctgaa gagttgattg atttatataa agaaagagaa atggcagaag
    20881 gttatcaata tggggaagac acagctgagc aaacaacatt tgaattagat tttccatatg
    20941 aacttacgcc tgaccaagct aaatctatcg atgaaattaa agatgacatg caaaaatcgc
    21001 gtccaatgga tcgcttgcta tgtggtgatg ttggttatgg taaaactgaa gttgcagtga
    21061 gagcagcatt caaagctgta atggaaggaa agcaggttgc atttttagtt cctacaacta
    21121 ttttagctca gcaacattat gagacgttaa ttgagcgtat gcaagatttt cctgttgaaa
    21181 ttcaattaat gagtcgtttt agaacgccta aagagataaa acaaactaag gaaggactta
    21241 aaactggatt tgttgacata gttgttggta cacacaaatt acttagtaaa gatatacagt
    21301 ataaagattt agggctgttg attgtagatg aagaacaacg atttggtgta cgccataaag
    21361 agcgtattaa aacattaaaa cataatgtag atgtactaac attgactgca accccaatac
    21421 ctagaacatt gcatatgagt atgctaggtg tgcgggattt gtcagtgatt gaaacgccgc
    21481 cagaaaatcg tttcccagtt caaacatatg tattagaaca gaacatgagt tttatcaaag
    21541 aagctttaga aagagaacta tcccgtgatg gccaagtgtt ttatctttat aataaagtgc
    21601 aatccattta tgaaaaacga gaacaactcc agatgttaat gccagatgct aacattgcag
    21661 ttgctcatgg acaaatgaca gagcgcgatt tagaagaaac gatgttaagt tttatcaata
    21721 atgaatatga tattttagta acgacgacga ttattgaaac aggtgtcgat gtcccaaatg
    21781 caaatacttt gatcattgaa gatgcagatc gctttggatt gagtcagttg tatcaattaa
    21841 gaggtcgtgt tggtcgttca agtcgtattg gttatgcata cttcttacat ccagcaaata
    21901 aggtactaac tgagactgca gaagatcgat tacaagcgat taaagaattt acggagttag
    21961 gctcaggatt taagattgcg atgcgtgatt tgaacattcg tggtgctggt aatttgttag
    22021 gtaaacaaca gcacggcttt attgatacag ttggatttga tttgtacagt caaatgttag
    22081 aagaagctgt aaatgaaaaa cgtggtatta aggaaccaga atctgaggtg ccagaagtcg
    22141 aagttgattt aaacttggat gcatatttgc caacagaata tattgcaaat gaacaagcta
    22201 aaattgaaat ttataaaaag ctacgaaaaa ctgaaacatt tgatcaaatt atcgacatta
    22261 aagatgaatt aattgatcgt ttcaatgatt atcctgttga agtagcacgt ttgcttgata
    22321 tagtggaaat aaaagtacac gcattacatt caggtatcac gttgattaaa gataaaggga
    22381 aaataattga tattcattta tctgtaaaag ccactgaaaa tattgatggc gaagtgctgt
    22441 tcaaagcaac acaaccttta ggtagaacaa tgaaggttgg tgttcaaaat aatgcaatga
    22501 caattacttt aacgaaacaa aatcaatggc ttgatagttt gaagttttta gttaagtgca
    22561 ttgaagaaag tatgagaatc agtgatgaag cataaagaag catttaatgg cgttgtcgtg
    22621 ttaactgctg cattaattgt cattaaaatt ctgagtgctg tatatcgaat tccatatcaa
    22681 aatatattag gcgatacagg tttgtatgca tatcaacaag tgtatccaat tgtagcatta
    22741 ggaatgatat tatcgatgaa tgccattcct agtgcaatta cacaaaatat agggaagtat
    22801 catagtgacg aagcatatgc aaaagcagtc gcttatatac aattagttgg tatattatta
    22861 tttattgcta tttttgtgtt tgcgaacaat attgcacata tgatgggtga tggccattta
    22921 acaccaatga ttcaagctgc aagtttaagc tttatattta taggtatgct tggcgtgtta
    22981 agaggttatt atcaatctgc aaataatatg acagttccgg ctatttccca ggttatagaa
    23041 caagttatac gagtaggtat tatcattgtt actattgtta tttttgtaga cagaggttgg
    23101 acgatatatg aagcgggaac aattgctatt ttagcatcaa cgataggttt tttaggttct
    23161 tcaatttatt tagtagcgca ccgacctttt aagtttaaaa tggtaaataa cactgcaaag
    23221 atcgtttgga aacagttcgc actttcggtt ttgattttcg ctatcagtca attaatcgta
    23281 attttatggc aagtgattga tagtgttact attattaagt cacttcaagc gatacgcgtg
    23341 ccattcgatg ttgccataac tgaaaaagga gtctatgacc gtggtgcatc atttattcag
    23401 atgggattga ttgtaactac aacatttagt tttgcgctca ttcctctgtt aagtgacgca
    23461 attaaaatga ataatcaggt acttatgaat cgttatgcaa atgcgtcatt aaagattacg
    23521 attttaataa gtacagcagc aggaatagga ttaattaatt tattgccttt aatgaacggt
    23581 gtgtttttta agacgaatga tttaacctta acgttaagtg tttatatgat tacggtcatt
    23641 tgtgtatcgt taattatgat ggatatggca ttattacaag cgcaacatgc tgtgagacct
    23701 atttttgttg gtatgacggc aggattggtt attaaattta tacttaatat cattttgatt
    23761 cgtttaagtg gcattattgg tgcgagcatt agtactgttg tatcattaat tatattcggt
    23821 acgattatcc atattgctgt cacgagaaaa taccacttat atgcgatgag acgatttttt
    23881 atcaatgttg ttttaggtat ggtatttatg tcgattgttg ttcaatgcgt gttaaacata
    23941 gtgacaacac acggtagaat cactggactc attgaattat tatgtgcagc agtattaggt
    24001 atcattgcat tgtttttcta tatttttaga tttaatgttt tgacatataa agagttaact
    24061 tatttaccat ttggttcaaa gttgtatcaa attaagaaag gaagacgttg atggcacata
    24121 ccattacgat tgttggctta ggaaactatg gcattgatga tttgccgcta gggatatata
    24181 aatttttaaa gacacaagat aaagtttatg caagaacgtt agatcatcca gttatagaat
    24241 cattgcaaga tgaattaaca tttcagagtt ttgaccatgt ttatgaagca cataaccaat
    24301 ttgaagatgt ctatattgat attgtggcgc aattggttga agctgctaat gaaaaagata
    24361 ttgtctatgc ggttccgggt catcctagag ttgctgagac aactacagtg aaattactgg
    24421 ctttagcaaa ggacaatact gatatagatg tgaaagtttt aggtgggaaa agctttattg
    24481 atgatgtgtt tgaagcagtt aatgtagatc caaatgatgg cttcacactg ttagatgcga
    24541 catcattaca agaagtaaca cttaatgtta gaacgcatac attgattacg caagtttata
    24601 gtgcaatggt tgctgctaat ttgaaaatca ctttaatgga acgatatcct gatgattacc
    24661 ctgttcaaat tgtcactggt gcacgaagcg atggtgcgga taacgttgtg acatgcccat
    24721 tatatgaatt ggatcatgat gaaaatgcat tcaataattt gacgagtgta ttcgtaccaa
    24781 aaatcataac atcgacatat ttgtatcatg actttgattt tgcaacggaa gtgattgata
    24841 ctttagttga tgaagataaa ggttgtccat gggataaagt gcaaacgcat gaaacgctaa
    24901 agcgttattt acttgaagaa acatttgaat tgttcgaagc tattgacaat gaagatgatt
    24961 ggcatatgat tgaagaacta ggagatattt tattacaagt gttattgcat actagtattg
    25021 gtaaaaaaga agggtatatc gacattaaag aagtgattac aagtcttaat gctaaaatga
    25081 ttcgtagaca cccacacata tttggtgatg ccaatgctga aactatcgat gacttaaaag
    25141 aaatttggtc taaggcgaaa gatgctgaag gtaaacagcc aagagttata tttgaaaaag
    25201 tatttgcaga gcatttttta aatttatatg agaagacgaa ggataagtca tttgatgagg
    25261 ccgcgttaaa gcagtggcta gaaaaagggg agagtaatac atgagattag ataaatattt
    25321 aaaagtatca cggttaataa agcgacgtac gctagcaaaa gaagtaagtg atcaaggtag
    25381 aattacaata aatggtaatg ttgctaaagc tggatcggat gttaaagttg aagatgtgct
    25441 gacgattcgc tttggtcaaa aattagtaac agttaaagta actgcattaa atgaacatgc
    25501 atctaaagat aacgcgaagg gtatgtatga aatcattgaa gagcgtcgac ttgaagaagc
    25561 gtaaattgga ggtgacaagc aatgaaaaat aaagtagaac atatagaaaa tcagtacacg
    25621 tcgcaagaga acaagaaaaa acaacgtcaa aaaatgaaaa tgcgtgttgt tcgtaggcgt
    25681 attacagtat ttgcgggcgt attacttgcg ataattgttg ttttatcaat cttgcttgtt
    25741 gtccaaaaac atcgcaatga tattgatgca caggagcgaa aagcgaaaga agcacagttt
    25801 caaaagcaac aaaatgaaga aattgcgtta aaagaaaagt tgaataatct gaatgacaaa
    25861 gattacattg aaaaaattgc gcgtgatgat tattacttaa gcaacaaagg tgaagtgatt
    25921 tttaggttgc cagaagacaa agattcgtct agctcaaaat cttcgaaaaa ataaatccaa
    25981 attgattcaa aattatccga gtatagacat tgtgaaaaaa tccaaacaag gatataataa
    26041 gggaaaatcg aatcaaatcg ggaggattta tttaacatat gtcaatcgaa gttggaaata
    26101 agcttaaagg taaagtcact ggtattaaaa agtttggtgc attcgtagaa ttacctgaag
    26161 gaaaaagtgg tttagttcac attagtgaag tcgcagataa ttatgttgaa aacgtagaag
    26221 agcacctttc tgttggtgat gaagtagacg taaaagtatt atctattgct gatgatggaa
    26281 aaattagtct ttcaattaag aaagctaaag accgtccacg tagacaacat acgagtaaac
    26341 caagtcatca aaaaccagtg caaaaagccg aagattttga aaagaaatta agcaatttct
    26401 taaaagatag tgaagataaa ttaacttcaa tcaaacgtca aacagaatct agacgcggtg
    26461 gcaaaggttc aagacgttaa ttaaaataaa taaagactgc ttcgataagg aatatattta
    26521 gaatgatgcg tatcgaataa tcgattgcag cgttagacaa tctaagactg tttcttaaat
    26581 aaggagcagt ctcttttatt tgtaatgata taactaagac ttataccatt tttgaaaatt
    26641 gtaaaagtga ggtgatgtta tgcagttaaa tagtaatggt tggcatgttg atgaccatat
    26701 tgttgtcgct gtttctacag gtattgatag tatgtgttta ttgtatcaac tactaaatga
    26761 ttataaagat agttatagaa aactaacatg cttacatgtc aatcatggcg ttaggtcagc
    26821 ttcaattgag gaagccagat ttttagaagc atactgcgaa cgtcatcaca tcgatttaca
    26881 tatcaaaaag ttagatttgt cgcatagtct cgaccgaaat aacagcattc agaatgaagc
    26941 tcgaattaaa cgttacgaat ggtttgatga aatgatgaat gtattagaag cggatgtatt
    27001 gctaacggcg catcatttgg acgatcaatt agaaactatt atgtatcgta tttttaatgg
    27061 gaaatcaaca cgtaataaac taggatttga tgagttatcg aagcgaaaag gttatcagat
    27121 ttatcgacca cttttagctg tctctaaaaa agaaataaaa caattccaag agagatatca
    27181 tattccatat tttgaagatg aatctaataa agataacaaa tatgttagaa atgatattcg
    27241 taatagaatt attccagcta ttgatgaaaa taatcaactt aaagtatcgc atttattaaa
    27301 attaaaacaa tggcatgatg aacaatatga tattttgcaa tattcagcta aacaatttat
    27361 tcaagaattt gtgaagtttg atgaacagtc aaaatattta gaggtttcta gacaagcttt
    27421 taataactta ccaaactcat taaagatggt tgtgttggac tgcctattat caaagtatta
    27481 tgagttgttt aatattagtg ctaaaacata cgaagagtgg tttaaacaat ttagtagtaa
    27541 gaaagcacaa ttcagtatta atctcacgga taaatggata attcaaatcg catatggtaa
    27601 attaataata atggctaaaa ataatggcga tacatatttt agagttcaaa caattaaaaa
    27661 gccaggtaat tatattttta acaaatatcg attagagata cattctaatt taccaaaatg
    27721 tttatttccg cttacagtga gaacacgaca aagtggcgat acatttaaac tgaatgggcg
    27781 cgatggttat aagaaagtga atcgcctgtt tatagattgt aaagtgccac agtgggttcg
    27841 ggatcaaatg ccaatcgtat tggataaaca acagcgcatt attgcggtag gagatttata
    27901 tcaacaacaa acaataaaaa aatggattat aattagtaaa aatggagatg aatagcgtta
    27961 tgcataatga tttgaaagaa gtattgttaa ctgaagaaga tattcaaaat atctgtaagg
    28021 aattgggagc acaattaaca aaggattatc aaggtaaacc attagtatgc gtgggtatct
    28081 taaaaggctc agcaatgttt atgtcagatt taattaaacg aattgatacc catttatcaa
    28141 ttgatttcat ggatgtttct agttatcacg gaggcactga gtcaactggt gaagttcaaa
    28201 tcattaaaga tttaggttct tctattgaaa ataaagacgt attaattatt gaagatatct
    28261 tagagactgg tactacactt aagtcaatta ctgaattatt acaatctaga aaagttaatt
    28321 cattagaaat agttacttta ttagataaac caaaccgtcg taaagcggac attgaagcta
    28381 agtatgtagg taaaaaaata ccagatgaat ttgttgttgg ttacggttta gattatcgtg
    28441 aattataccg aaacttacca tatatcggta cgttaaaacc tgaagtgtat tcaaattaat
    28501 tttttaatca atttcagtta ttattactat gcgtttgaga aataatagtg tagactcaaa
    28561 aatatgaaaa atgtatttca tatatattta attttagaca agacatatgt cttgaaaagt
    28621 tgaaaaatat agagattgat aaaactaata cgggtgtgaa tgacattgat gttaagctca
    28681 attactagct tataaaacat gtcatatgtt acaatttttg ttagttttat tatgggaagt
    28741 aggaggaaat gacgcatgca gaaagctttt cgcaatgtgc tagttatcgt aataataggc
    28801 gttattattt ttggtctatt ttcatattta aacggtaatg gaaatatgcc gaaacagctt
    28861 acatataatc aatttactga gaagttggaa aaaggtgacc ttaaaacttt agaaatccaa
    28921 ccacaacaaa atgtctatat ggtaagtggt aaaacgaaaa atgatgaaga ctattcatca
    28981 actattttat ataacaacga aaaagaatta caaaaaatta ctgatgctgc taaaaagcaa
    29041 aacggtgtaa aattaacgat taaagaagaa gaaaaacaaa gtgtctttgt gagtatactt
    29101 tcaacattaa ttccagttgt agtcatagcg ttattattta ttttcttcct aagccaagca
    29161 caaggtggcg gtagtggcgg tcgtatgatg aactttggta aatctaaagc aaaaatgtac
    29221 gataataata aacgtcgtgt tcgtttctct gatgtagcag gggcagatga agaaaaacaa
    29281 gaattaattg aaattgttga tttcttgaaa gataataaaa aattcaaaga aatgggatct
    29341 aggattccta aaggtgtctt acttgttgga cctccaggta ctggtaaaac attacttgct
    29401 agagcggttg caggtgaagc tggcgcacca ttcttctcta ttagtggttc agactttgta
    29461 gagatgtttg ttggtgttgg tgcgagccgt gttcgtgact tattcgataa tgctaagaaa
    29521 aacgcgcctt gtatcatctt tatcgatgag attgatgctg ttggtcgtca acgtggtgca
    29581 ggtgttggtg gcggtcatga tgaacgtgaa caaaccctaa accaattatt agttgaaatg
    29641 gatggtttcg gtgaaaatga aggtatcatt atgatagctg ctacaaaccg tcctgatatc
    29701 cttgacccag ccttattacg tccaggtcgt tttgatagac aaattcaagt tggtcgtcca
    29761 gatgtgaaag gccgtgaagc aattcttcat gttcatgcta aaaacaaacc acttgatgaa
    29821 acggttgatt taaaagcaat ttcacaacgt acacctggtt tctcaggtgc tgatttagag
    29881 aacttattaa atgaagcatc tttaattgct gtacgtgaag gtaaaaagaa aattgacatg
    29941 agagatatcg aagaggcaac ggatagagtt atagccggac ctgctaagaa atctcgagtt
    30001 atttctaaga aagaacgtaa tattgttgct catcacgaag ctggtcatac aattatcggt
    30061 atggtacttg atgaggcaga agtagtgcat aaagttacta ttgttccacg tggacaagca
    30121 ggtggttatg caatgatgct acctaaacaa gatcgtttct taatgactga acaagagtta
    30181 ttagataaaa tctgtggttt acttggtgga cgtgtatcag aagatattaa ctttaacgaa
    30241 gtatcaacag gtgcttcaaa tgacttcgaa cgtgcaacac aaatcgcacg ctcaatggtt
    30301 acgcaatatg gtatgagtaa aaaattagga ccattacagt tcggtcatag caatggtcaa
    30361 gtattcttag gtaaagatat gcaaggtgag cctaattatt caagccaaat cgcatatgaa
    30421 attgataaag aagttcaacg aatcgttaaa gaacaatacg aacgttgtaa acaaatttta
    30481 ttagagcaca aagaacaatt aattttaatt gctgaaacat tattaacaga agaaacatta
    30541 gttgctgaac aaattcaatc attattctac gaaggtaaat tacctgaaat tgattatgat
    30601 gcagctaaag ttgttaaaga tgaagattct gaatttaatg atggtaaatt cggtaaatct
    30661 tatgaagaga ttcgtaaaga gcaattagaa gatggacaac gtgacgaaag tgaagatcgt
    30721 aaagaagaaa aagatattgc tgaggataaa aaagaagctg ataaatctga tgaaaaagat
    30781 gaaccagcac atcaacaagc cccaaatatc gaaaaacctt acgatccaaa tcacccagac
    30841 aataaataat cgattatatt cagtacctct ttctatgata aagttataga aagaggtact
    30901 tttatcgttt ttgaaaatac gtattagatt ttaagtcgtt gaattgttat agcagaaaat
    30961 aattgtaaaa caagttactt cattatttag aatgatgggt gtagaataag tacaattgtt
    31021 gcattttatg aagtaaagta attttttaaa tatagagtaa tagaggagat tgaaataatg
    31081 acacacgatt atattgttaa agcattagca tttgatggag agattagggc ttatgctgct
    31141 ttgacaactg aaactgttca agaagcacaa acgagacatt atacatggcc gacagcatct
    31201 gctgcaatgg gaagaacaat gacagcaaca gctatgatgg gcgcaatgtt gaaaggtgat
    31261 caaaaattaa ctgtcactgt agatggccaa ggacctattg gacgaattat tgccgatgca
    31321 aatgctaaag gcgaggtgcg tgcttatgta gaccatccac aaactcattt tccattaaat
    31381 gagcaaggta aacttgatgt aagacgagcg gtagggacaa atggatctat tatggttgtt
    31441 aaagacgttg gaatgaaaga ctatttctct ggagcaagtc caattgtttc aggagaactt
    31501 ggtgaagatt ttacttatta ttatgctaca agtgaacaaa caccttcatc ggtaggtctt
    31561 ggtgtattgg taaatcctga taatacgatt aaagcagcag gaggatttat cattcaagtt
    31621 atgccaggtg ccaaagatga aacaatttca aaattagaaa aagcaattag tgaaatgaca
    31681 ccagtttcta aattaattga acaaggatta acgccagaag gattactaaa cgaaatctta
    31741 ggtgaagacc atgtgcaaat tttagagaaa atgcctgttc aatttgaatg taattgtagt
    31801 catgagaaat ttttaaatgc tattaaagga ttgggcgagg ctgagattca aaatatgatt
    31861 aaagaagatc atggtgctga agcagtatgt catttctgtg gaaataaata taaatatact
    31921 gaagaagaat taaacgtgtt gctagaaagt ttagcgtaat ttaatttaaa tcaatacgct
    31981 aaaatgttta tttttagcgg tttagtgaaa tgtagaacta aatagttgta taatccttag
    32041 tgattttgtt tgctttctag aatttatttg ataaaataat tctatatccg ataaataaac
    32101 taagatttca acaactaact aaaaaggagt gttcttaatg gcacaaaaac cagtagataa
    32161 tattactcaa attattggcg gtacaccggt agtcaaattg agaaatgtag tagatgacaa
    32221 tgcagcagat gtttatgtaa aattggaata tcaaaatcca ggtggttctg taaaggatag
    32281 aattgcttta gcaatgattg aaaaagcaga gcgagaaggc aaaattaaac ctggcgatac
    32341 aattgtagaa ccaacaagtg gtaatacagg tatcggttta gcatttgtat gtgctgctaa
    32401 aggatataaa gcagtattta ctatgcccga aacaatgagc caagagcgtc gtaatttatt
    32461 aaaagcatac ggtgcggaat tagttttaac gcctggatca gaagcgatga aaggtgcaat
    32521 taaaaaagct aaagaattga aagaagaaca tggttacttc gagccacaac aatttgaaaa
    32581 ccctgcgaac cctgaagttc atgagttaac tacaggtcct gagttattac aacaatttga
    32641 agggaaaact atcgatgcgt tcctagctgg tgttggtact ggtggtacgt tatctggtgt
    32701 aggtaaagtt ctgaaaaaag aatatcctaa catcgaaatt gttgctatag agcctgaggc
    32761 ttctccagta ttgagcggtg gtgagccagg tccacataaa ttacaaggtt taggtgctgg
    32821 atttattcca ggcactttga atacagaaat ctatgacagt attattaaag taggaaatga
    32881 tacagcgatg gaaatgtctc gtcgagttgc taaagaggaa ggtattttag caggtatttc
    32941 atcaggtgct gcgatttatg ctgccattca aaaagcaaaa gaattaggaa aaggtaaaac
    33001 agtagtaaca gtattgccga gtaatggtga acgctactta tcaacacctt tatattcatt
    33061 cgatgactaa ttaatgtcat ttaaaagagt gagttatctt tttgagataa cttgctcttt
    33121 ttttctacca tgtatatttt taaaaatatg agcgttaaat taaacatttt tctgataaaa
    33181 atatccagtg aatgataaga taataaacgt acatactaat aactagtaaa tagcaggagt
    33241 aaattttatt agagttaaac aatacataat taaagggtgg ttaacatgac taaaacaaaa
    33301 attatgggca tattaaacgt cacacctgat tcattctcag atggtggaaa atttaataat
    33361 gttgaatcag ctataaatag agtgaaagcc atgatagatg aaggtgctga cattatagat
    33421 gttggaggtg tttcaacgag acccggtcat gaaatggttt cattagaaga agagatgaac
    33481 agagtattac ctgttgttga agctattgtc ggttttgatg taaaaatttc agtcgataca
    33541 tttcgaagtg aggttgctga agcatgttta aaattaggcg ttgatatgat taatgatcaa
    33601 tgggcgggtc tgtatgatca tcgtatgttc caaattgtag ctaaatatga cgcggaaatt
    33661 attttaatgc ataatggaaa tggtaatcgt gatgaaccgg ttgtcgaaga aatgttaaca
    33721 tctttgttag cacaagcaca tcaagctaaa atagctggta taccttcaaa taaaatttgg
    33781 ctagatccag gtataggttt cgctaaaact agaaatgaag aagccgaagt tatggcaaga
    33841 ctggatgaac ttgttgcaac agaatatcca gttttattag cgacaagccg gaaacgtttc
    33901 actaaagaga tgatgggtta tgatacaaca ccggttgaaa gagatgaagt aactgcagct
    33961 acgactgcat atggtattat gaaaggcgtt agagcagtac gcgttcataa tgtcgagttg
    34021 aatgctaaat tagctaaagg tatagatttt ttaaaggaga atgaaaatgc aagacacaat
    34081 ctttcttaaa ggtatgcgct tttatggata tcatggtgct ttatcagctg aaaatgaaat
    34141 agggcaaatt ttcaaagtgg atgtaacttt gaaagtagac ttagctgaag ctgggcgtac
    34201 tgataatgtt attgatacag ttcattatgg tgaagtgttc gaagaggtta aatcaattat
    34261 ggaaggtaag gccgttaatt tacttgagca tctagctgaa cgtattgcaa atcgtataaa
    34321 ttcacaatat aatcgtgtaa tggaaacgaa agtgagaatc actaaagaaa acccaccgat
    34381 tccgggtcat tatgatggag taggtatcga aatagtgagg gagaataaat gattcaagca
    34441 tacttaggat taggtagtaa tattggtgat agagaaagcc agttaaacga tgctataaag
    34501 attttgaatg aatatgatgg tattaacgta tctaatattt ctccgattta tgaaacagca
    34561 ccagttgggt atactgagca acctaacttt ttaaatttgt gtgttgaaat tcaaacaaca
    34621 ctcacagtat tacaactgtt ggaatgttgt ttgaagacag aagaatgttt acaccgtatt
    34681 agaaaggaac gatggggtcc tagaacttta gatgtggata ttttgttgta tggagaagaa
    34741 atgatagatt taccaaaact gtcggtgcca catccgagaa tgaatgaacg tgcatttgtt
    34801 ttaatcccat taaatgatat agcagcaaat gtcgtagaac cacgttcgaa attgaaagtg
    34861 aaagatttag tttttgtcga tgacagtgta aagagatata aataatgtat tgttgagaac
    34921 attcatattt attgggaata gattattgat tattggagat gtttccgcat tttgattatc
    34981 tctaaatgca tttgatttcg aattagtata tgatgattta gtatgtgtta gaattaagtt
    35041 atttcataaa atcaaagtaa gcaatatttg ataaattgct gtaatttatt taggcttttg
    35101 ttgaagtttg tggaagataa tttttataca tttacttagg gttgtattaa tgataatcgt
    35161 attttaaatg aataagtaat tcataaacga aaacagaaat tgctttatta catttacaag
    35221 ctcttgtaaa attaaaaaag tgataaaatt gatgttatga atataagaaa acatgccaca
    35281 tcataacttt aaggtgtaat ggttaatgat aaagtattag aaacatcgaa agagatgtat
    35341 gttgagcaaa aatgtctgat attttataaa actttaaagg aaaatgtttg agtgtaccag
    35401 ttggaatact aaaggattac aacaagttaa aggagagaaa gttatgtcag aagaaatgaa
    35461 tgaccaaatg ttggttcgac gtcaaaaatt acaagaatta tatgatcttg gtatagaccc
    35521 gtttggttct aaatttgacc gttcaggttt atctagtgat ttgaaagaag agtgggacca
    35581 gtattctaaa gaagaattgg tagaaaaaga agcggatagt catgtcgcta tagctggacg
    35641 attaatgact aagcgtggta aaggtaaagc aggatttgca cacgttcagg acttagctgg
    35701 acaaattcaa atttacgttc gtaaagatca agttggcgat gacgaatttg atttatggaa
    35761 aaatgctgat ttaggcgata tcgttggtgt tgaaggtgta atgttcaaaa caaatactgg
    35821 cgaattatcg gttaaagcga agaaattcac gctactaact aaatcattgc gaccattacc
    35881 ggataaattc cacggtttac aggatattga acagagatat cgtcaaagat atttagattt
    35941 aattacgaac gaagatagca ctcgtacatt tattaatcgt agtaaaatca ttcaagaaat
    36001 gcgtaattat ttaaataata aaggtttctt ggaagtagaa acacctatga tgcaccaaat
    36061 tgctggtgga gcagctgcta gaccatttgt aacacatcat aatgcattag atgcaacgtt
    36121 atacatgcgt attgctattg agttgcattt aaaacgttta attgtcggtg gacttgaaaa
    36181 agtatatgaa attggtagag tattccgtaa tgaaggtgta tcaactagac ataaccctga
    36241 attcacaatg attgaattat atgaagcata tgcagattat catgacatta tggatttaac
    36301 agaatctatg gtgagacata ttgccaatga agtgttaggt tctgcaaaag tacaatacaa
    36361 tggggaaacg atcgatttag aatctgcttg gactcgtttg catattgttg atgctgtaaa
    36421 agaagctact ggtgtagatt tttatgaagt taaaagtgat gaagaagcta aagctttagc
    36481 taaagaacat ggtattgaaa ttaaagatac aatgaaatat ggtcatattt taaatgaatt
    36541 ctttgagcaa aaagttgaag aaacacttat tcagccaacg tttatctatg gtcatccgac
    36601 tgaaatttca cctttagcga agaaaaatcc tgaagatcct agatttactg atcgtttcga
    36661 attgttcatt gtaggtagag agcatgcaaa tgcatttact gaattaaatg atcctattga
    36721 tcaaaaaggt cgttttgaag cgcaacttgt tgaaaaagcg caaggtaatg atgaagcgca
    36781 tgaaatggat gaagattaca ttgaagcgtt agaatatggt atgcctccga caggtggtct
    36841 tggtatcggt attgacagat tggttatgtt attaactgac tctccatcaa tcagagacgt
    36901 attattattc ccttatatga gacaaaaata aatgacgttg attgttagta agagctctcg
    36961 tgtatacaac atgtgtatgc gagggctttc ttaattatgg taattagttc gtgtttgaat
    37021 gtttttgata gtaatagtta acgatagtgg tgctattttt gactgttaaa caaggtagtt
    37081 ggctgataga tgaaaatgac gatacgtata tagagtagtt cggatgagaa atgttaacga
    37141 tagacattga gatatcttat aacaaaaatt gctatattag tataatttat cttaatcagc
    37201 tataaaagta ctttaaaatt gtatagaatg tgtatggttt tgtacatatg tgtatgatag
    37261 aatactaaaa gtgttatgaa ttagagggca cgagaaatgt cagttttgaa gaataaaaaa
    37321 gttgattaaa agtgttgact ttatcaattg aatgaagtaa tatataaaag tcgccaaaaa
    37381 cagacgaaac acactaaaag ctgatgtgac aaagtttaca tcaaagtgta aaattaacta
    37441 ttgcacctta ttaattaagc gtgtatcatg aataagtaag ttattttgtc tggtgactat
    37501 agcaaggagg tcacacctgt tcccatgccg aacacagaag ttaagctcct tagcgtcgat
    37561 ggtagtcgaa cttacgttcc gccagagtag aacgttgcca ggcaaatgac aaatcggaga
    37621 attagctcag ctgggagagc atctgcctta caagcagagg gtcggcggtt cgaacccgtc
    37681 attctccacc atttattctt agatatagcc ggcctagctc aattggtaga gcaactgact
    37741 tgtaatcagt aggttggggg ttcaagtcct ctggccggca ccatcttttg agccattagc
    37801 tcagctggta gagcatctga cttttaatca gagggtcaga ggttcgaatc ctctatggct
    37861 cattacgatt taatttttat atttagcaaa ataatgcaga agtagttcag cggtagaata
    37921 caaccttgcc aaggttgggg tcgcgggttc gaatcccgtc ttctgctcca ttattttgcc
    37981 ggggtggcgg aactggcaga cgcacaggac ttaaaatcct gcggtgagag atcaccgtac
    38041 cggttcgatt ccggtcctcg gcaccatttt agcgcccgta gctcaattgg atagagcgtt
    38101 tgactacgga tcaagaggtt atgggttcga ctcctatcgg gcgcgccatt tttaaattaa
    38161 ttgaataacg ggaagtagct cagcttggta gagcacttgg tttgggacca aggggtcgca
    38221 ggttcgaatc ctgtcttccc gattacttct taaattccat tttatggggg cttagctcag
    38281 ctgggagagc gcctgctttg cacgcaggag gtcagcggtt cgatcccgct agtctccacc
    38341 atttattttt tacacgatga acattgaaaa ctgaatgaca atatgtcaac gttaattcca
    38401 aaaaacgtaa ctataagtta caaacattat ttagtattta tgagctaatc aaacatcata
    38461 atttttatgg agagtttgat cctggctcag gatgaacgct ggcggcgtgc ctaatacatg
    38521 caagtcgagc
//
LOCUS       DALAAR010000020        38351 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000020 DALAAR010000000
VERSION     DALAAR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.020"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            61..3315
                     /locus_tag="O2458_001039"
     CDS             61..3315
                     /locus_tag="O2458_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCZ3886912.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTP
                     VKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
     gene            3434..4906
                     /locus_tag="O2458_001040"
     CDS             3434..4906
                     /locus_tag="O2458_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ3886913.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5030..6520)
                     /locus_tag="O2458_001041"
     CDS             complement(5030..6520)
                     /locus_tag="O2458_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ3886914.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6802..7680)
                     /locus_tag="O2458_001042"
     CDS             complement(6802..7680)
                     /locus_tag="O2458_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCZ3886915.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(7693..8358)
                     /gene="bshB2"
                     /locus_tag="O2458_001043"
     CDS             complement(7693..8358)
                     /gene="bshB2"
                     /locus_tag="O2458_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCZ3886916.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(8372..8734)
                     /locus_tag="O2458_001044"
     CDS             complement(8372..8734)
                     /locus_tag="O2458_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCZ3886917.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            9010..9768
                     /gene="nagB"
                     /locus_tag="O2458_001045"
     CDS             9010..9768
                     /gene="nagB"
                     /locus_tag="O2458_001045"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCZ3886918.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            9845..10477
                     /gene="hxlA"
                     /locus_tag="O2458_001046"
     CDS             9845..10477
                     /gene="hxlA"
                     /locus_tag="O2458_001046"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCZ3886919.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            10479..11027
                     /gene="hxlB"
                     /locus_tag="O2458_001047"
     CDS             10479..11027
                     /gene="hxlB"
                     /locus_tag="O2458_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCZ3886920.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            11140..11787
                     /locus_tag="O2458_001048"
     CDS             11140..11787
                     /locus_tag="O2458_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3886921.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            12289..13689
                     /locus_tag="O2458_001049"
     CDS             12289..13689
                     /locus_tag="O2458_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3886922.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            14233..15609
                     /locus_tag="O2458_001050"
     CDS             14233..15609
                     /locus_tag="O2458_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ3886923.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            15611..16750
                     /locus_tag="O2458_001051"
     CDS             15611..16750
                     /locus_tag="O2458_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ3886924.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            16725..17090
                     /locus_tag="O2458_001052"
     CDS             16725..17090
                     /locus_tag="O2458_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCZ3886925.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            17093..17362
                     /locus_tag="O2458_001053"
     CDS             17093..17362
                     /locus_tag="O2458_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886926.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(17563..17730)
                     /locus_tag="O2458_001054"
     CDS             complement(17563..17730)
                     /locus_tag="O2458_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCZ3886927.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(18237..19067)
                     /gene="thiD"
                     /locus_tag="O2458_001055"
     CDS             complement(18237..19067)
                     /gene="thiD"
                     /locus_tag="O2458_001055"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ3886928.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            19251..19907
                     /locus_tag="O2458_001056"
     CDS             19251..19907
                     /locus_tag="O2458_001056"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCZ3886929.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            19908..20288
                     /locus_tag="O2458_001057"
     CDS             19908..20288
                     /locus_tag="O2458_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCZ3886930.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            20421..20789
                     /locus_tag="O2458_001058"
     CDS             20421..20789
                     /locus_tag="O2458_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCZ3886931.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            20910..22394
                     /locus_tag="O2458_001059"
     CDS             20910..22394
                     /locus_tag="O2458_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ3886932.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(22536..22988)
                     /locus_tag="O2458_001060"
     CDS             complement(22536..22988)
                     /locus_tag="O2458_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ3886933.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(23001..23765)
                     /locus_tag="O2458_001061"
     CDS             complement(23001..23765)
                     /locus_tag="O2458_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ3886934.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(24314..25066)
                     /locus_tag="O2458_001062"
     CDS             complement(24314..25066)
                     /locus_tag="O2458_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCZ3886935.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            25234..26220
                     /gene="pta"
                     /locus_tag="O2458_001063"
     CDS             25234..26220
                     /gene="pta"
                     /locus_tag="O2458_001063"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCZ3886936.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            26223..27059
                     /locus_tag="O2458_001064"
     CDS             26223..27059
                     /locus_tag="O2458_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ3886937.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            27646..28566
                     /gene="mvk"
                     /locus_tag="O2458_001065"
     CDS             27646..28566
                     /gene="mvk"
                     /locus_tag="O2458_001065"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCZ3886938.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            28571..29554
                     /gene="mvaD"
                     /locus_tag="O2458_001066"
     CDS             28571..29554
                     /gene="mvaD"
                     /locus_tag="O2458_001066"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCZ3886939.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            29567..30643
                     /locus_tag="O2458_001067"
     CDS             29567..30643
                     /locus_tag="O2458_001067"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCZ3886940.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            30820..31161
                     /locus_tag="O2458_001068"
     CDS             30820..31161
                     /locus_tag="O2458_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCZ3886941.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(31213..32535)
                     /locus_tag="O2458_001069"
     CDS             complement(31213..32535)
                     /locus_tag="O2458_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCZ3886942.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(32522..32962)
                     /locus_tag="O2458_001070"
     CDS             complement(32522..32962)
                     /locus_tag="O2458_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ3886943.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            33020..33121
                     /locus_tag="O2458_001071"
     CDS             33020..33121
                     /locus_tag="O2458_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886944.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            33587..34996
                     /locus_tag="O2458_001072"
     CDS             33587..34996
                     /locus_tag="O2458_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ3886945.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            35000..35626
                     /locus_tag="O2458_001073"
     CDS             35000..35626
                     /locus_tag="O2458_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3886946.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            35752..36393
                     /locus_tag="O2458_001074"
     CDS             35752..36393
                     /locus_tag="O2458_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ3886947.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            36481..37098
                     /locus_tag="O2458_001075"
     CDS             36481..37098
                     /locus_tag="O2458_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3886948.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            37173..37802
                     /locus_tag="O2458_001076"
     CDS             37173..37802
                     /locus_tag="O2458_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ3886949.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            37859..37975
                     /locus_tag="O2458_001077"
     CDS             37859..37975
                     /locus_tag="O2458_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886950.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            37998..>38351
                     /locus_tag="O2458_001078"
     CDS             37998..>38351
                     /locus_tag="O2458_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3886951.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     ICCITFIGILTFYVRL"
BASE COUNT        13606 a         5631 c         6852 g        12262 t
ORIGIN      
        1 aatttttata tgtaaaaata ttaatttttt gcaaatattg atattttaaa ggagatttat
       61 atgattaaca gggataataa aaaggcaata acaaaaaagg gtatgatttc aaatcgctta
      121 aacaaatttt cgattagaaa gtatactgta ggaactgcat cgattttagt aggtacgaca
      181 ttgatttttg gtctagggaa ccaagaagct aaagctgctg aaaacactag tacagaaaat
      241 gcaaaacaag atgatgcaac gactagtgat aataaagaag tagtgtcgga aactgaaaat
      301 aattcgacaa cagaaaataa ttcaacaaat ccaattaaga aagaaacaaa tactgattca
      361 caaccagaag ctaaaaaaga atcaacttca tcaagtactc aaaaacagca aaataacgtt
      421 acagctacaa ctgaaactaa gcctcaaaac attgaaaaag aaaatgttaa accttcaact
      481 gataaaactg cgacagaaga tacatctgtt attttagaag agaagaaagc accaaataat
      541 acaaataacg atgtaactac aaaaccatct acaagtgaac catctacaag tgaaattcaa
      601 acaaaaccaa ctacacctca agaatctaca aatattgaaa attcacaacc gcaaccaacg
      661 ccttcaaaag tagacaatca agttacagat gcaactaatc caaaagaacc agtaaatgtg
      721 tcaaaagaag aacttaaaaa taatcctgag aaattaaaag aattggttag aaatgatagc
      781 aatacagatc attcaactaa accagttgct acagctccaa caagtgttgc accaaaacgt
      841 gtaaacgcaa aaatgcgctt tgcagttgca caaccagcag cagttgcttc aaacaatgta
      901 aatgatttaa ttaaagtgac gaagcaaaca atcaaagttg gcgatggtaa agataatgtg
      961 gcagcagcgc atgacggtaa agatattgaa tatgatacag agtttacaat tgacaataaa
     1021 gtcaaaaaag gcgatacaat gacgattaat tatgataaga atgtaattcc ttcggattta
     1081 acagataaaa atgatcctat cgatattact gatccatcag gagaggtcat tgctaaagga
     1141 acatttgata aagcaactaa gcaaatcaca tatacattta cagactatgt agataaatat
     1201 gaagatataa aatcacgctt aactctatat tcgtatattg ataaaaaaac agttccaaat
     1261 gagacaagtt tgaatttaac atttgctaca gcaggtaaag aaacaagcca aaatgtcact
     1321 gttgattatc aagatccaat ggtccatggt gattcaaaca ttcaatctat ctttacaaaa
     1381 ttagatgaag ataagcaaac tattgaacaa caaatttatg ttaacccatt gaaaaaatca
     1441 gcaaccaaca ctaaagttga tatagctggt agtcaagtag atgattatgg aaatattaaa
     1501 ctaggaaatg gtagcaccat tattgaccaa aatacagaaa taaaggttta taaagttaac
     1561 tctgatcaac aattgcctca aagtaataga atctatgatt ttagtcaata cgaagatgta
     1621 acaagtcaat ttgataataa aaaatcattt agtaataatg tagcaacatt ggattttggt
     1681 gatattaatt cagcctatat tatcaaagtt gttagtaaat atacacctac atcagatggc
     1741 gaactagata ttgcccaagg tactagtatg agaacaactg ataaatatgg ttattataat
     1801 tatgcaggat attcaaactt catcgtaact tctaatgaca ctggcggtgg cgacggtact
     1861 gttaaacctg aagaaaagtt atacaaaatt ggtgactatg tatgggaaga cgttgataaa
     1921 gacggtgttc aaggtacaga ttcaaaagaa aaaccaatgg caaacgtttt agttacatta
     1981 acttacccgg acggtactac aaaatcagta agaacagatg ctaatggtca ttatgaattc
     2041 ggtggtttga aagacggaga aacttataca gttaaattcg aaacgccaac tggatatctt
     2101 ccaacaaaag taaatggaac aactgatggt gaaaaagact caaatggtag ttcggttact
     2161 gttaaaatta atggtaaaga tgatatgtct ttagatactg gtttttacaa agaacctaaa
     2221 tacaacttag gtgactatgt atgggaagat actaataaag atggtatcca agatgcaaat
     2281 gagccaggaa tcaaagatgt taaggttaca ttaaaagata gtactggaaa agttattggt
     2341 acaactacta ctgatgcctc gggtaaatat aaatttacag atttagataa tggtaactat
     2401 acagtagaat ttgaaacacc agcaggttac acgccaacgg ttaaaaatac tacagctgat
     2461 gataaagatt ctaatggttt aacaacaaca ggtgtcatta aagatgcaga taatatgaca
     2521 ttagacagtg gtttctataa aacaccaaaa tacagtttag gtgattatgt ttggtacgac
     2581 agtaataaag acggcaaaca agattcaact gaaaaaggta tcaaagatgt gacagttaca
     2641 ttgcaaaacg aaaaaggcga agtaattgga acaactaaaa cagatgaaaa tggtaaatat
     2701 cgtttcgata atttagatag cggtaaatac aaagttattt ttgaaaagcc tgctggctta
     2761 acacaaacag ttacaaatac aactgaagat gataaagatg cagatggtgg cgaagttgac
     2821 gtaacaatta cggatcatga tgatttcaca cttgataacg gatacttcga agaagataca
     2881 tcagacagcg attcagactc agatagcgac tcagactcag acagcgactc agattcagat
     2941 agcgattcgg actcagatag cgactcagat tcagacagcg attcagactc agatagcgac
     3001 tcagactcag acagtgattc agattcagac agcgactcag actcagatag cgactcagat
     3061 tcggacagcg actcagactc tgatagcgac tcagactcag acagtgattc agacagcgat
     3121 tcagactcgg atgcaggaaa acatacacct gttaaaccaa tgagtactac taaagaccat
     3181 cacaataaag caaaagcatt accagaaaca ggtagtgaaa ataacggctc aaataacgca
     3241 acgttatttg gtggattatt tgcagcatta ggttcattat tgttattcgg tcgtcgcaaa
     3301 aaacaaaaca aataatacaa tatgacccag gtccttgtgg cctggttttt ttataattac
     3361 acatgcaata gatgtatttt tcatataaaa taaacaataa agatacggaa taaaacttat
     3421 atgaggcgat aatatgaatt acattttagg aacaatttta gaaagtaaaa ttacaggtgt
     3481 agaaaaagcg caaataaata gattgaagtt gttcaaacaa cacggcatat cttcaaaatg
     3541 tgtatatgtt aaatggaatc cttattcata cacatatgcg aagcaacatc agattgaaaa
     3601 tgatgtattt acaatgtatg actattttca aaaagcaatc aattataaaa agacaaagca
     3661 agttaactgg atacagtatt gggaaaagtc atgtaggtac acattgaaat ttgtggaaaa
     3721 ttcaaatgat gtcagaatat atgatgaaga gcaatttgta atgtatgctc attttttaga
     3781 taaacagtat catcaattaa attatgtgaa ttattttgat cataaaagaa gaaaagtaaa
     3841 acgcgaattg tatgatggaa gaggcttttt aagttgttct cgaattttag gtgaaggaca
     3901 acggattgta ctcgaaaatt actatacacc taatggggaa atcgtcatcc aaaaatattt
     3961 tgacgatata aaagggaaaa acacgctcac aaaggttatc ttaaatgaag accagcatca
     4021 acaatttttt gatacagaag atgaattagt tcaatatttt ctccatcaat tatgtaaaaa
     4081 taatgatcaa atcatattag atcgtcctca tgaattagga aatgttatag cgggattaaa
     4141 tcaaagtatt ccagttattg ttgtactcca tagtacacat ttatccggtg ccggtaatgg
     4201 tataaaaagt ttttataaaa cagtgtttaa taatttaaca cgttataaag cgattgttgt
     4261 atcaacagaa aagcaatgcc aagatatttc acaatatatt gaaaataaaa taccagttat
     4321 caatattccg gttggctacg tggcaaattt aaagtatcaa tttgacatca atcaaaagga
     4381 gaaaaatcat atcatatcaa ttgctcgcct cgttgaaaat aaacaaatta aacatcaaat
     4441 tgaagtaatc aagcaattag taacaaaaca tcccaatatt caattgaata tttatggaca
     4501 tggaaatggt ttgtcagaat atcgacaact tgtagaagat tatcatttat cggaacatgt
     4561 taaatttcat ggttttaaga cgcatattaa tgaagagatt gctaaagcag aactgatgtt
     4621 atcgacaagt aaaatggaag gttttggctt agcaatttta gagtcgcttt cagtaggtac
     4681 accagtgatc agttatgatg tagattatgg tccatcagaa ctgattcaag atggatttaa
     4741 tggctattta gtacctcaag gtgacatcaa tcaaatggtt gaaaaggtcg accaattact
     4801 aaataatact caaaaattgc aacagttttc aattaatagc atagaatctg cacaacagta
     4861 caatgcaact actatcagta caaagtggca aaatatttta aactaagtca aaagaaaaaa
     4921 gcattttcct agaatcatct atttaacaaa cctcgttcat acgaacttac atgtttagtt
     4981 aaatgacata ggaaaatgct atttttgtaa aaataaaggt aaagtacatt tatataagac
     5041 gaacaaattg gtcccattgt ttaattaacg acgctttact atattgttgt gcttttgcca
     5101 aactaccttt tgacagtcgt tgctgtactt caggatgatc aatcacatat tttactttat
     5161 caaatagggc atcttcatca tttttagtaa ttaaataacc attgaaatct gaagtaatca
     5221 gttcgttagg tccatattta atatcataac taataactgg aacaccatgt gctaaagatt
     5281 caagtagcgc taaagagaaa ccttccatgt tacttgttat taaactcaaa taggcatcgc
     5341 tatattcttg gtctagattg cttaaaaagc cgcgtaagta aacatgattt tccaatccat
     5401 atttttgtat caattcattt aattttttac tttcagaacc aaaaccatac atatgaagct
     5461 ctatttttgg gacatacgat actaagcgtt taattaattc aatttgttga tgtaattgtt
     5521 tttcaggtga ataacgagca acggaaatta atttaacact gcgctgatct aatgtttgga
     5581 ctggtgtatc aattgtttca ctatagccga caggaatatt aacaactgga atagtatggt
     5641 taatacgttt ttcaacatct aatttttgct gctcagtaga aacgataatt gcacgatatc
     5701 gagataaatt ttcaaacatc gctttatata catttttaaa tggcgatgaa tctaatgcat
     5761 caatattttt aatgtgtgta ctgtgaagca cagctactac tgggattgac tcaggcgtta
     5821 agttgaaaat aggtgctgtg tacacattac gatcactgaa aaataaatcc ccatgttgat
     5881 atagttgttt aatgaaaaat gcgcctaatt ccgtttcatt attaaagaaa tattgtttgt
     5941 tagcatagta aacaataatt ttttgtactt ctggtttgcc atccttgtaa gaaaaatact
     6001 tttctaattt tgtgtcacct tctggattat agaaaaattc acataatgtt tgttgtttat
     6061 caacaagaat cctactacaa cttaaaaagc cacgcacatc ataaaaatca cgttttactt
     6121 ttcgtctttg actatcaaaa tgatttacat aatctaatat acgatattta ggatcttgaa
     6181 aatgggcata cattaagaaa cgctcttgat catatattct aaagtcatga ctattttcaa
     6241 catgttttaa agtataatga cattcatcag tccaatacga caaccagtca aatggttcat
     6301 tgcgttctaa atatgttgct tcttggaaga aatcatacat attaatatag tcagaactag
     6361 taatataatt ttgggcattt ctatataaat atctattcca tgacagaaat acacattgcg
     6421 ctggtcttcc catttcttta aataaattta aacgattaat aattgctttc tctatcccag
     6481 ttaaattaac acctaaacta ttacctacaa aataattcat ttacaacacc acttatatct
     6541 attttttata attatatcac ataatattta attacttctt ttaactggaa gatgtgttta
     6601 tttataaaac aacaaatttt gatatttata atgatagtag ttattcaatc aactacgacc
     6661 aatatatcat tgtagagctt aggatattga tttatgactc aggcacatca aatgagaaga
     6721 tttataaaag agatatacaa ctctagaagg tataataaaa acgcgcaact aatgttacgc
     6781 gtttgaatta atcatatgat attatttgcg atactttaat ttagcgaaag catcatgttg
     6841 atggatagac tcttcattac gacattcgat atcgaaaccg tctaaccaat caaattcaac
     6901 taagtccgcg gcaattaaac gaattaagtc ttcgacaaaa cgtggatttt catatgcacg
     6961 ctctgtcaca cgtttttcat caggacgttt taaaataggg tatagaattg aacttgcatt
     7021 agcttccatt gcatctaaaa ttttattttt atagtcatca actatgtctt gatctttatt
     7081 aatatatgtt ttaacagtga caacaccacg ttggttgtgc gctgaatact cacttatttc
     7141 ttttgaacaa gggcatagcg ttgtgacagt tgcttcaata gtaagttctt tacgtgtaac
     7201 tttatcaccg tcaattgcta atccataagt gacatcggca ttaccaactg ctttaatatt
     7261 tgtggttgga ctatagcgat caaagaacca tttcccagaa acatcaacgc ctgccgcatt
     7321 ttgtttcata ttcgtttgta aagtgcgtaa cacctgataa agtgtattaa attcaagttc
     7381 aataccatta tcatagtgct tttcaacact ttcgattata cggctcatat taataccttt
     7441 ttcgtctttt gttaaacttg ttgaaaaact aaatgtgcca gctgtttgat actggtcaac
     7501 aagtacaggg tacactaagt ttttaatacc aacttcttct atttcaaata aaaaatcttt
     7561 atgtgtactt tgtaaatctg tcatttcgtt cttagtagta ggtttcgtgc cttcaatagg
     7621 atctacggaa ccaaagtgtt tccaacgacc ttctcgtgtc gataaatcaa attcagtcat
     7681 ttttttcctc cgttaagatt taaagtgata tgtccaatat ggttcgactg ttaaaaagct
     7741 gtgttgttta ccatcgattt caggacttgc taattgtttt aaaaatggac ctgtttgaga
     7801 agcatgtgct tcaaatgcct taattttaag ttctttaaaa tctgtaatat cattttgaat
     7861 atcaggttct ccaagagctt cggttgcatc attactgaac gcaactaaag ttaaacgagg
     7921 gcgttcttct ttaggcatgc gttcaaccgt tcgaattaca gcgtctgctg ttgcttcgtg
     7981 atcaggatgt actgcatatc caggataaaa tgaaataatc aatgatggat ttgtatcatc
     8041 gattaaagat ttaatcatac catctatatg ttcatagggt tcaaattcga cagttttgtc
     8101 acgtaaaccc atttttctta aatcagtaat accgataact ttacaagctt cttctagttc
     8161 acgctcacga atacttggta atgattcgcg tgttgcaaat gggggattac ctaaatttct
     8221 gcccatttgt cctagggtta aacatgcata tgttacaggt atgccttttt ggatataatt
     8281 tgctaatgtg cctgcagatg agaaggtttc atcatcagga tgtggaaata ttactaatac
     8341 atgtctttcg tcagtcatgt tgatgcctcc tctataaatt aaatggtcgc tcactaattt
     8401 gaagtgctgc agcgagttga ccttcgtaat taaaacctgc aattaaaaat tcatcatgct
     8461 cattgacctc aaaatgcgtt agaccttgta cataaaccca accaccattt gatagtttaa
     8521 gaccaatgcg ataaggttct ttattaccac cttttagttg tgcatgcgta tatgttattt
     8581 gtatgtttct taaaaaagta ccagcattaa aaacacgttg atcgaaatgg ttcgcatagg
     8641 ccccatttgt cgtttcaaca tgcagataca caggtttatg ttcaaaagaa gcaagtaaat
     8701 ctataacttc ttgttcttta attggttcca acacgttcac tccttacact atcaatgtgt
     8761 ttatctttct attttactaa aaactattcg ataattgtat acgattgctc aattatttat
     8821 aaattaattt tcatgaaggg taattactca ggattacgta atcatacagc attagttttt
     8881 tacttttaaa aatcaaaaat ttgttggaat ttgaaaagtg ttaaacatta aaaatgatgc
     8941 tatattaatg gtgtatgaat gaattcataa gtttttaaaa tgtattaaat ttgtggaggc
     9001 atgtaaacaa tgaaagtatt aaacttagga tcgaaaaaac aagcatcatt ctatgttgca
     9061 tgtgagttat ataaagagat ggcatttaat cagcactgta aactaggttt agcaactggt
     9121 ggtacaatga cagatttgta tgaacaactt gttaagttat taaataaaaa tcagttaaac
     9181 gtagacaatg tatccacgtt taatttagac gaatatgtag gtttaaccgc atcacatccg
     9241 caaagttatc actattatat ggatgacatg cttttcaaac aatatcctta ttttaataga
     9301 aagaacattc atattccaaa tggagatgcc gatgatatga atgcggaagc gtcaaaatat
     9361 aatgacgttt tagaacaaca aggtcaacgt gatattcaaa ttttaggtat tggtgaaaat
     9421 ggtcatattg gatttaatga acctggtacg ccgtttgata gcgttactca tatcgttgat
     9481 ttgactgaaa gtactattaa ggctaatagt cgatatttta aaaacgaaga tgatgttcca
     9541 aagcaagcca tttcgatggg acttgctaat attcttcaag ccaaacgtat cattttactc
     9601 gcatttggtg aaaagaaacg tgctgctatt acacatttat taaatcagga aatttctgtt
     9661 gatgttccag ccacattact tcacaaacac ccgaatgttg agatatattt agacgacgaa
     9721 gcttgcccga aaaatgttgc gaaaattcat gtcgatgaaa tggattgatt gcaatgttta
     9781 attaagaaat gcctcgggaa aggttccaat agaaagataa aaagcattgg aaggatgatt
     9841 tttagtggaa ttacaattag caattgattt attaaacaaa gaagacgcgg ctgagttagc
     9901 aaataaagta aaagattatg tagatatcgt agaaatcggt acgccaatca tttacaacga
     9961 aggtttacca gcagttaaac atatggcaga caacattagt aatgtaaaag tattagcaga
    10021 catgaaaatt atagatgcag ctgattatga agttagccaa gcaattaaat ttggcgcgga
    10081 tgtaattaca atactaggtg ttgcagaaga tgcatcaatt aaagcagcta ttgaagaagc
    10141 tcataaaaat aataaacaat tactagttga tatgattgct gttcaagatt tagaaaaacg
    10201 tgcaaaagaa ctagatgaaa tgggtgctga ttatattgca gtacacactg gttatgattt
    10261 acaagcagaa gggcaatcac cattagaaag tttaagaacc gttaaatctg ttattaaaaa
    10321 ttctaaagtt gcagtagcag gtggaattaa accagataca attaaagata ttgtcgctga
    10381 aagtcctgat cttgttattg ttggtggcgg aatcgcaaat gcagatgatc cagtagaagc
    10441 tgcaaaacaa tgtcgcgctg caatcgaagg taagtaatat ggctaaattt agtgactatc
    10501 aattaattct agatgaatta aagatgactt tgtcacatgt tgaagcggat gagttttcaa
    10561 cttttgcatc caaaatacta catgctgaac atatatttgt agctggcaaa ggacgttcag
    10621 gattcgtggc gaatagtttt gcaatgcgct taaatcagct cggcaaacag gcacatgttg
    10681 ttggagaatc aacgacacct gcgattaagt cgaatgatgt atttgtaatt atctctggtt
    10741 caggttccac ggaacattta agattattag cagacaaagc aaaatcagta ggtgctgaca
    10801 tcgtattaat tactacaaat aaagattctg caataggcaa tctagctggg acgaacatcg
    10861 ttttgcctgc aggtacaaaa tatgatgaac aaggctcggc acaaccatta ggaagtttgt
    10921 ttgaacaagc atctcaatta tttttagata gtgttgtaat gggattgatg actgaaatga
    10981 atgttacgga acaaacgatg caacaaaatc atgctaattt agaataaaat aaagatagtc
    11041 gataatatga tgcctaggca gaaatattat cgattatttt tttatttaaa taataaatta
    11101 tagtataata tcaataataa acgaataggg gtgttaatat tgaagtttga caattatatt
    11161 tttgattttg atggtacgtt ggcagacacg aaaaaatgtg gtgaagtagc aacacaaagt
    11221 gcatttaaag catgtggctt aacggaacca tcatctaaag aaataacgca ttatatggga
    11281 atacctattg aagaatcatt tttaaaatta gcagaccgac cattagatga agcagcatta
    11341 gcaaagttaa tcgatacatt tagacataca tatcaatcta ttgaaaagga ctatatttat
    11401 gaatttgcgg gtataactga agccattaca agtttgtata accaagggaa aaaacttttc
    11461 gtggtgtcta gtaagaagag tgatgtatta gaaagaaatt tatcggctat tggattaaat
    11521 cacttgatta ccgaagctgt tggatccgat caagtaagtg catataaacc aaatcctgaa
    11581 ggcatacaca caattgtgca acgctacaat ttaaatagcc aacaaacggt gtatattggt
    11641 gattcaacgt ttgatgttga gatggcacaa cgtgctggta tgcaatctgc agctgtcact
    11701 tggggtgcac atgatgcaag gtcattactt cattcaaatc cggattttat tattaatgat
    11761 ccatcagaaa ttaataccgt attataaaac ttgttaaaac agagaatacc atggttaaaa
    11821 tgcatattca taaatattag attatactta gaaatatttc gctttagatt aggaatttaa
    11881 aataaatatt tattaaacat tatgaatttt taaagagtaa tgtctgactc gttgataatt
    11941 tatttttgta aaaataaatt aaagtaatga caaagttatt gaagtaaatt gagtataaac
    12001 atttaaatac gatgtcgaaa atggcgatag catatcactt acatgaagtt atgtgctatc
    12061 gctattttta gttataattc caaaaagtta atcgttcgat gatttaagaa ttattattgt
    12121 ttaattcaaa tgtatgaggg tataaaatca ttgaatttaa ttcgataaag cgaaattttt
    12181 gaacaaacat acttttgtat ttatataaaa gtttaaattc ttataaattt gacaaaacta
    12241 attaactccg tataattatg aaacatacaa gagggagtgt atgaattcat ggattttaat
    12301 aaagagaata ttaacatggt ggatgcaaag aaagctaaaa aaaccgttgt tgcaaccggt
    12361 atcggtaatg caatggaatg gttcgatttt ggtgtctatg catatacaac tgcgtacatt
    12421 ggagcgaact tcttctctcc agtagagaat gcagacattc gacaaatgtt gactttcgca
    12481 gcattagcca ttgcgttttt attaagacca attggtggtg tcgtatttgg tattattggt
    12541 gacaaatatg gacgtaaagt tgtattaaca tctacaatta ttttaatggc attttcaaca
    12601 ttaaccattg gattattgcc aagctatgat caaattggac tttgggcacc aatactatta
    12661 ttgcttgcaa gagtactaca agggttttca acaggtggag agtatgcggg ggcaatgaca
    12721 tatgttgccg aatcatctcc agataagcgt cgtaactcat taggtagtgg actagaaatt
    12781 gggacattat caggttacat agctgcttca attatgattg ctgtattaac attcttttta
    12841 acagatgaac aaatggcatc atttggttgg agaatcccat tcttactcgg tttattccta
    12901 ggattattcg gcttatattt acgtcgtaag ctggaagaat caccagtttt cgaaaatgat
    12961 gttgcaacac aaccagaaag agataacatt aactttttac aaatcatcag attttattac
    13021 aaagatatat ttgtatgttt tgtagctgtt gtattcttca atgttacaaa ctatatggta
    13081 actgcatatt taccaaccta tttagaacaa gttattaaat tagatgcaac gacaacaagt
    13141 gtattaatta cttgtgtcat ggcaataatg attccattag cattaatgtt tggtaagtta
    13201 gcggataaaa taggtgaaaa gaaagtattt ctaattggta ctggtgggct aacattattc
    13261 agtatcatcg catttatgtt attacattca caatcatttg ttgtaatagt aatcggtata
    13321 tttatattag gatttttctt atcaacttac gaagcgacaa tgccagggtc gttaccaacg
    13381 atgttttaca gtcatataag atatcgaact ttatcagtaa catttaatat ctctgtttcg
    13441 atatttggtg gtacgacgcc attagttgca acatggttag ttacgaaaac tggagatcca
    13501 ttagcacctg cgtattattt aacagcaatc agtgttattg gctttttagt tattacattc
    13561 ttacatttaa gtacagcagg aaaatctcta aaaggttcgt atccaaatgt agataacgag
    13621 caagatagag cttattatgc agaacatcca aaagaagcat tatggtgggt taaagaacgt
    13681 aagaattaga gattttaata aaaagtataa atcaatcgta tataagcact ttaaagctag
    13741 taggttctgc taactttaaa gtgcttttta aattgagaac tgtaattagc cgtaataaag
    13801 tttttgtata tacataaacc cccactgcaa tgattatcgc aatgggggaa agaggggact
    13861 taaagcatat gtttagcttt gaatacttaa aattctcttg ctattgaaat gttaggatgt
    13921 aaatatgtct tagagtattt tgtccaacgc aattaatatt gagactctaa ccttcaatat
    13981 tattatagag aacacaaact taaatagatt gggtgactta tttgtgtcag ttattgcgat
    14041 tgcgataact tcttttctct atatacatat agtaacgtct tatctaataa aaaacatggt
    14101 actacagtat caaatttatc tagggcttaa gtttgatttt tataataggc aggtttacct
    14161 gataaaaata cttattcatt atataatgtt aacaatatgt attttaaagt ttacattgag
    14221 tgagggatat tgatgaacgt aattttagaa cagttgaaaa cacatactca aaataaacct
    14281 aatgacatag cattacatat cgatgatgaa acaattacat atagtcaact aaatgcccgc
    14341 atcactagcg cagttgaatc tttgcagaaa tattcactta accctgtcgt tgctattaat
    14401 atgaaatcac cggtgcaaag tattatttgt tatttagctt tgcatcgttt acataaagtg
    14461 cctatgatga tggaaggtaa atggcaaagt actatacatc gtcaattgat tgaaaaatat
    14521 ggtattaaag atgtaattgg agatacaggt ctcatgcaga atatagactc accgatgttt
    14581 attgattcaa cgcaattaca gcactacccc aatttattac atattggttt tacttcaggg
    14641 acaactggac tgccaaaagc atattatcgt gatgaagatt catggttggc ttcttttgaa
    14701 gttaatgaaa tgttgatgtt aaaaaatgaa aatgcaatag cagcccctgg accactatcg
    14761 cactcgttaa cattatatgc gttattgttt gctttaagtt ccggtcgtac ttttatagga
    14821 cagaccactt ttcatcctga aaagttactt aatcaatgtc ataaaatatc atcatacaaa
    14881 gttgctatgt ttcttgttcc aacgatgatt aaatcattat tgttagttta caacaatgaa
    14941 catacaatcc aatcattttt tagcagtgga gataagctgc attcttctat ttttaaaaag
    15001 ataaaaaatc aagcaaatga cataaatttg attgaatttt ttggtacatc ggaaaccagt
    15061 tttatcagct ataacttgaa tcagcaagca ccagttgaat cagtaggtgt gctatttcca
    15121 aatgtggaat tgaaaacaac gaatcacgat cacaatggta taggaactat ttgtataaaa
    15181 agtaatatga tgtttagtgg ctatgtaagt gaacaatgta taaataatga tgaatggttt
    15241 gttactaatg ataatggcta tgtaaaagag cagtatttat atttaacggg acgtcaacag
    15301 gatatgttaa ttattggtgg tcaaaatata tatccagcac atgttgaacg ccttttaacg
    15361 caatcttcga gcattgatga agcaattatc atcggtattc caaatgagcg ttttggtcaa
    15421 ataggcgtat tgctttattc tggtgatgtg acacttacac ataaaaatgt aaaacaattt
    15481 ttaaaaaaga aagtgaaacg ctatgaaatt ccatcgatga ttcatcatgt agaaaagatg
    15541 tattacactg caagtggtaa aattgctaga gaaaaaatga tgtcgatgta tttgagaggt
    15601 gaattataat atgaatcaag cagtcatagt tgcagctaaa cgaactgcat ttgggaaata
    15661 tggtggcact ttaaaacatt tagagccaga acaattgctt aaacctttat tccaacattt
    15721 taaagagaag tatccagagg taatatctaa aatagatgat gtagttttag gtaatgttgt
    15781 tgggaatggt ggcaatattg caagaaaagc attgcttgaa gcggggctta aagattcaat
    15841 acctggcgtc acaatcgatc ggcaatgtgg gtctggactt gaaagtgttc aatatgcatg
    15901 tcgcatgatc caagccggag ctggcaaggt atatattgca ggtggtgttg aaagtacaag
    15961 tcgagcacct tggaaaatca aacgaccgca ttctgtgtac gaaacagcat tacctgagtt
    16021 ttatgagcgt gcatcatttg cacctgaaat gagcgaccca tcaatgattc aaggtgctga
    16081 aaatgtggcc aagatgtatg atgtttcaag agaattacaa gatgaatttg cttatcgaag
    16141 tcatcaattg acagcggaaa atgtaaaaaa tggaaatatt tctcaggaaa tattacctat
    16201 aaccgttaaa ggagaaatat tcaacactga tgaaagtcta aaatcacata ttccgaaaga
    16261 taactttggc cgatttaagc ccgtgatcaa aggtgggacc gttaccgctg cgaatagttg
    16321 tatgaaaaat gatggtgcag ttttattgct tattatggaa aaagatatgg catacgaatt
    16381 aggtttcgag catggtttat tatttaaaga tggtgttacg gtaggtgttg attctaattt
    16441 tcctggcatt ggtccagtac cagccatttc caacttacta aaaagaaatc aattaacgat
    16501 agaaaatatt gaagtcattg aaattaacga agcgttcagt gcacaggtag ttgcctgcca
    16561 acaagcttta aatatttcaa atacgcaatt aaatatatgg ggtggtgcat tagcatcagg
    16621 tcatccatac ggtgcaagcg gtgcccaatt agtgactcga ttattttata tgtttgacaa
    16681 agagactatg attgcatcta tggggatagg gggaggtcta ggaaatgcag cattatttac
    16741 tcgattctaa ccagcgatta aatgtgtcat tttctaagga tagtgtggct gcatattatc
    16801 agtgttttaa ccaaccttat agaaaagaag taccaccatt aatgtgtgcg tcattatggc
    16861 caaaatttga tttatttaaa aaatatgcaa atagcgaact gattttaaca aaatcagcaa
    16921 ttaatcaaac tcaaaagata gaagtagaca caatatatgt agggcattta gaagatattg
    16981 aatgccgaca gactcgcaat atcacacgtt atacaatggc tttaacatta actaaaaatg
    17041 atcaacatgt cataacggtt acacaaactt ttattaaggc gatgaagtag agatgaagtt
    17101 taatgagata tggataaatg aatatttggc gctcgtaaat gatgataatc caatacataa
    17161 tgagattgtg ccaggacaat tagtgagtca aatgatgctg atggctatgt cattagagac
    17221 aaaccagtgt caaattaact acgttaaacc tattttaata aatgaaaata tcgaattcat
    17281 tgaacaacac gaacacgaaa ttatagcaat taatgacgat ggagagatta aaataaaaat
    17341 ttctttgagc acaaaaaaat aaccgatatt agctgcatga acgcatatta attaggagat
    17401 gaaaggacag ctaatatcag ttatgtattg ttattattat tgggaacaga gatgaatata
    17461 ggttacgttt ctttctttgc acggggatgc attaatctaa aataataata acaactatat
    17521 caatgtttaa taaattctgg attattggaa cgattagtca atttaactaa ctttcatatg
    17581 atctatatcg tcttgtaata aagagagcaa tttgaatatt tcagtatcac taaatgaatc
    17641 gtcacattta attgaaacat gctgaaacgt tttggttata atttcataaa ctggtgcgcc
    17701 ttcatggtga tactgtcgat aaataatcat aacctatatt acctcctttg ctactctatg
    17761 gttatattat aaataacatt tttatgtgtg acatcaacct taagtatcaa ctttttatca
    17821 gacatagaac gtatgattta ctaagactat ttatgtataa aagttctaaa taaatatata
    17881 tttatagagt cgcctggcag tcatttggga aatataacat atatgattag agaggcatct
    17941 atcgcaaaag aatgataatg atagaggtat tgagcatata gatgagttta agttcatctt
    18001 gaaaataaag ggttatttag tcatagatgt agatgtatag gaaatatttg tatgtattgt
    18061 tcgatatgta tgaaattttc aataaaagct aataacgctt atatgtaact ttcaaattta
    18121 aattatatac agagcatgat gattataaaa aaataaccac atcacataaa ttgagttcat
    18181 acccaattta agtggtgtgg ctaataatgt tgatttatag atgaaccgcc taatcgttaa
    18241 acctctgtta cttcaacatc gatatgttca atacggttgt atgcaccgtg atccacagga
    18301 ccaacaaaat cattcatttt ccaaccgttt ttaatagcag aagcgacgaa agctttcgcg
    18361 ctaatcacag cttctttcgg tgacttaccg ttagctaaat atgcagttgt tgccgcagca
    18421 aatgtacaac cagcaccatg gttataactt tgttggaaca tgtctgttgt tagttgataa
    18481 aatgtttgac catcatagta taagtcatac gatttatctt gatctaaagc tttgccacct
    18541 ttaatgatga catgctgtgc gcctttatca aagataattg ttgcagcctt tttcatatct
    18601 tcaattgaat ttaatttacc taatcctgat aattgacccg cttcaaataa gtttggtgtc
    18661 actaccgttg ctttaggtag taaatattta atcatcgcct cagtatttcc aggattaagc
    18721 acttcatctt cgcctttaca aaccatgaca ggatctacta caaaatattg tgcattagat
    18781 gcctcatata cttctccagc acgtttgatt atctcctcag tacctaacat acctgtttta
    18841 atagcatcag gtccgattga taaagccgtt tcaagttgtt tttcaaatac atccattggt
    18901 aatggtgtaa catcgtgtga ccatgtatct ttatccatag taacgatggc agttaaagcg
    18961 accatgccat acgtatctaa ttcttggaac gttttcaaat ctgcttgcat acctgcgcca
    19021 gcacttgtgt cagaaccggc aattgttaaa actttcttta aagccattga gcttcactcc
    19081 tacataataa tattgtattc atcatatcat ttttaaccta attgaaaaat attaagcatt
    19141 caatatttga tgattgttga aatgaatcat tcatactatt gtaacttttg aaaatgtcat
    19201 tcactttaga taagtgtgat atgttaaaat atgtcctgag gtgagattga atggaatggt
    19261 cgcaaatttt tcatgacata acaacgaaac atgactttaa agctatgcat gattttttag
    19321 aaaaagaata ttcgactgca atcgtatacc ctgataggga aaatatatat caagcgtttg
    19381 atttaacacc gtttgaaaat atcaaagttg ttatattagg acaagacccg tatcatggtc
    19441 caaaccaagc acatggatta gcattttcag tgcaacctaa cgcaaaattc cctccatctt
    19501 tacgtaatat gtataaagaa ttagcagatg atattggatg cgttagacaa acaccgcatt
    19561 tacaagattg ggcaagagaa ggcgtcttgt tattgaatac agttttaacc gtaagacagg
    19621 gtgaagcaaa ttctcatcgt gatattggtt gggaaacatt tactgatgaa attattaaag
    19681 cagtgtctga ttataaagaa catgttgtct ttattttgtg ggggaaacct gcacagcaaa
    19741 aaataaagct tatcgataca tctaaacatt gtattataaa atcagtgcat cctagtccac
    19801 tgtctgcata tagaggattc tttggatcaa aaccgtattc caaagcgaat gcctatttag
    19861 agtcagtagg aaaatcacca attaattggt gtgaaagtga ggcgtagatg ttgaatagag
    19921 aaactttaat agcacgaatt gagcaagaat tagtacaagc agagcaggca cagcatgacc
    19981 atgactttga aaaacatatg tatgccatac atatattaac atctttatat gcttcaacat
    20041 caaatacacc acatattggt gaacaacaaa tgaatcgtcg tattgctaac cataatcaaa
    20101 tgccacaatc acaaataacg cagccaactc atcaagtgac agttgctgaa attgaagcga
    20161 tgggtggtaa agtaaatacg cattcagcac atcatcataa taagtcatat tcacaacctt
    20221 caaaccaaca acaaagatta gcgacagatg atgacattgg caatggtgaa tccatatttg
    20281 atttttaaaa agcaacaatg aaacataatt acttaatagc ttgttaagta tgtaggttaa
    20341 taatcaagac gcatatactt ttattcgagt gttcggattt aaacatttat taatactgaa
    20401 ttatataagg agaggtagca atgaaattat ttattatttt aggtgcatta aacgcgatga
    20461 tggctgtcgg tacaggtgca tttggtgcgc atggtttaca aggaaaaata agtgatcact
    20521 atttatcagt atgggaaaaa gcaacgacgt atcaaatgta ccatggctta gcattattaa
    20581 ttataggtgt aattagtggt acaacttcaa tcaatgttaa ctgggctggc tggttaatat
    20641 ttgctggtat tattttcttt agtggatcat tatatatttt agtattaact caaattaaag
    20701 ttttaggtgc gattacgcca attggtggcg tattgttcat cattggatgg ataatgttaa
    20761 tcattgcgac attcaaattt gctggttaaa ttttaaaact ttagattacc tatgtaacta
    20821 aacattaaat ttttaataaa aataatcaag aaaaagagtt acaaactcat cttttgggta
    20881 tagaatacct tcgaggtgag tttttattta tggaaaaaaa gaataagcaa atagatagag
    20941 gcgatttaaa acaaaaccta tctgaaaagt ttgtatgggc gattgcatat ggttcatgta
    21001 tcggatgggg cgcattcatc ttaccaggag actggattaa gcagtcaggt ccgattgcag
    21061 catcaattgg tatagttatt ggtgcattat taatgatatt aattgcggtt agttatggcg
    21121 cattagtaga gagatttcca gtatcagggg gcgcgtttgc ctttagtttc ttaagtttcg
    21181 gcagatatgt gagtttcttc tcatcatggt ttttaacttt tggttatgtc tgtgtcgttg
    21241 ctttaaatgc gaccgcattc agtttactag ttaaattctt attgccagat gtcttaaata
    21301 atgggaaact atacaccatt gcgggctggg acgtttatat tacggaaatc attattgcga
    21361 ccgtattact acttgtattc atgctagtaa cgattcgtgg cgcaagtgta tctggatcat
    21421 tacaatatta tttctgtgtg gcgatggtaa tcgtcgtatt attgatgttc tttggttcat
    21481 tctttggtaa taattttgca cttgaaaatt tacaaccgtt agctgaacct agcaaaggat
    21541 ggttagtgtc tattgtggtt attgtatccg tggcaccatg ggcatatgtt ggatttgata
    21601 atattccaca aacagcagaa gagtttaact ttgcaccaaa caagacattt aagcttatcg
    21661 tgtacagttt attagcagca tcattaactt atgttgtcat gattttatac actggttggt
    21721 tatcaacaag tcatcaaagt ttaaatgggc agttgtggtt aacaggtgct gttacacaaa
    21781 cagcatttgg ttatattgga ttaggtgtat tagcaattgc aattatgatg ggtatattta
    21841 ctggtttaaa tggattcttg atgagttcaa gtcgcttgtt attttctatg ggacgttcag
    21901 gtattatgcc aacaatgttt agtaaattac atagtaaata caaaacacca tatgtcgcaa
    21961 tcatattcct agtaggagtg tcgttaattg caccttggct aggaagaact gcattgactt
    22021 ggattgtaga tatgtcatct actggtgtat ccattgccta ctttattaca tgtttgtctg
    22081 cagcgaaatt attcagttat aacaaacaaa gtaatacgta tgcaccggtt tacaaaacgt
    22141 ttgctattat cggctcattt gtatcattca ttttcttagc gttgttatta gtgccaggtt
    22201 ctcctgcagc actgactgca ccgtcttata ttgcattact tggatggtta atcatcggtt
    22261 taatattctt tgtgattcga tatcctaaat tgaaaaatat ggataatgat gaattaagtc
    22321 gcttgatttt aaatagaagt gaaaatgaag ttgatgatat gattgaagaa cctgaaaaag
    22381 aaaaaactaa ataataaaag aatcgcacaa taaaccttct tcattcggag gcgtatcgtg
    22441 cgattttttg tattataaat tgacatttaa gacgaggcag ctgaacctta tatataattg
    22501 ctaagagtta gggctgagcc atttctaaca aatatttata atcgtttaaa agatttcacg
    22561 aacccagaaa caattaattt ggaaatttgg tcggcgaata ataaacctaa tgcgatggcg
    22621 cctgcaataa gtgtaacctc tagcatggta ttgattgctg tactgaaatt taataagact
    22681 aaattttttg tagcatcgta tgctaagcca ccaggtacta atggaatgat acccgttacc
    22741 ataaaaatga tggcaggttc tttttgttta cgagccatat aatgacttaa caagcctaat
    22801 gctaaactac caaagaaact agagtatata gtgtgcacat taaagccgtt gaagaataag
    22861 gtgtaaacca tccatccaca cgtaccaacg aaaccacatg atagatataa ttttctaggt
    22921 gcatcaaaaa tgacgcagaa gaacattgaa gctaaaaagc taaagataaa gtttaagatc
    22981 caaaacatag tctgatactc ctatactaaa attaatacgc taccaacgcc agcaccgatg
    23041 ccaaacgcag taaccaatgc ttctaatgat ttcgttgtga acatcaacat gtgtccacca
    23101 aataaatctt gtattgcgtt tgttattaat acaccaggaa caataggcat gactgccgca
    23161 atgataatag ttgccaagtc acctgttgga ataagtgtat gtccaataac ggcgataatc
    23221 ccaataacta atgaaccaat gaattctggg ataaactgtg cgtgtaactt acgatctaaa
    23281 atctcagtga ctaggtatcc tagactacct gctaatatcg cagttaaaac atcaatcaat
    23341 ctaccacctt gtaaatataa gaaactcatt gcaatcattg ctgcagcaaa acctttaaag
    23401 ggaagactgc tgtcacgctt agcaacatat attttttcaa gttgcgtttt tgcttcggct
    23461 aaagaaattt cattgtttgt aatttgacgc gaaattttat tagcttgaga aatttttatt
    23521 aagtttgtat ctcgagaggt aattctaaat attctaggaa acgattccga atgtaacgta
    23581 aactggatga cagtgtttgt aacaaagctg ttactttcac tgtaaccaag tttttttgca
    23641 atacgtgtca tggtatcttc tacacgcgta ccttctgcac cagattctaa tagtatgcga
    23701 gcagcaagca tgacaacgtc tttgataagt acctcttgtt tgtattcttc tgaatttatg
    23761 tccatttcat caccattgtt tataagaatt taatactcat tatagtttat acactataaa
    23821 ataaccacat gagctttttg ataagttgtt attgatttta aaaaactaat atttgatact
    23881 attgtaataa taatcatcgg aatttttagt agttaaaatt ctcttgaagt ataaaaagga
    23941 aaaattattg aaaaaaattc gacaaaaata tgtttcaaat atttcaaata ggttaaaata
    24001 atattagtta accattacaa aaattgtata gagtagcgac tgtataattt ctattgaggt
    24061 taacgtttat atgtagtgat agtagttaaa gttctcccaa ggaagactac tcgggtacac
    24121 tttgctatga gcaaagtgta ctttgttatt gataatacat tagcacatat atataagttt
    24181 aaacatacag atttcaacta tttaccaaat cacgtcttat gtatacggga atatactgaa
    24241 gataaacgaa aaattccaag cttaaaccga taagcttgga atttttttat taatttataa
    24301 acgtaccaat gtattaagaa atcgcaaaga attgatcgaa ttcgtttgtg ttaataatat
    24361 gtcctacaaa gaaactaccg aattcaccgt atcgtgctgt tgtttcatca aagcgcattt
    24421 cgtatacaat ttttttgaat tgtaatacgt catctgagaa caatgttacg ccccattcga
    24481 aatcatcaaa ccctacagaa ccagtaataa attgtttgat tttgccagca tattttctac
    24541 caatcatacc atggtcatac attaattttt ggcgttcttc catagttaac atgtaccagt
    24601 tataagtttc attacgacgt ttgttcattg gatagaaaca aatataatca gaatgtggta
    24661 attctgggta taatcttgct ttgatatgag ggttctcata aggatcttca tcagatttac
    24721 cagctaaata attgctcaat tcaatgactg atacatatga atatgtaggg attaggaagt
    24781 cagcaatgcg caatttgtta aattcatttt caatatgatt taaagacttc atttcaggac
    24841 gtaagaacca taataacaaa tctgcttttt gaccagttat attataaata gcttgatcac
    24901 cagattttga tgatcttaca gttgctgtat tttctaaaaa tgattgaaat tcagtgacaa
    24961 gtgcatcgcg ttcgtccttt ggaactatac gtaatgatgc ccaatcaact gcataaaata
    25021 aatgtagact ataccaacca tctaatgttt cggctgcttg actcatgttt atcgctcctt
    25081 ttcaaaaatc atcagttctt tataaatttt atcataaagc aaaggggtaa cgataaaatt
    25141 atgattacaa attggtgacg tggcattatg aaataaaatg gcgtataatt ataccgtgaa
    25201 tgattaataa gatttatatt acaggaggac attatggctg atttattaaa tgtattaaaa
    25261 gacaaacttt ctggtaaaaa cgttaaaatc gtattacctg aaggagagga cgaacgtgtt
    25321 ctaacagctg caacacaatt acaagcaaca gattatgtta caccaatcgt gttaggtgat
    25381 gagactaagg ttcaatcttt agcgcaaaaa cttgatcttg atatttctaa tattgaatta
    25441 attaatcctg cgacaagtga attgaaagct gaattagttc aatcatttgt tgaacgacgt
    25501 aaaggtaaag cgactgaaga acaagcacaa gaattattaa acaatgtgaa ctacttcggt
    25561 acaatgcttg tttatgctgg taaagcagat ggtttagtta gtggtgcagc acattcaaca
    25621 ggcgacactg tgcgtccagc tttacaaatc atcaaaacga aaccaggtgt atcaagaaca
    25681 tcaggtatct tctttatgat taaaggtgat gaacaataca tctttggtga ttgtgcaatc
    25741 aatccagaac ttgattcaca aggacttgca gaaattgcag tagaaagtgc aaaatcagca
    25801 ttaagctttg gcatggatcc aaaagttgca atgttaagct tttcaacaaa agggtctgct
    25861 aaatcagacg acgtgacaaa agttcaagaa gctgtcaaat tagcacaaca aaaagctgaa
    25921 gaagaaaaat tagaagcaat cattgatggc gaattccaat ttgatgctgc gattgtacca
    25981 ggtgttgctg agaaaaaagc gccaggtgct aaattacaag gtgatgcaaa tgtctttgta
    26041 ttcccaagtt tagaagctgg taatattggt tacaaaattg cacaacgttt aggtggatat
    26101 gatgcagttg gtccagtatt acaaggttta aattctccag taaatgactt atcacgtggc
    26161 tgctcaattg aagatgtata caatctttca attattacag cagcgcaagc cttacaataa
    26221 cgatggattt agcgagtaaa tattttaatg gcgtcaactg gcgatatatc gatcattctt
    26281 ctggattaga acctatgcaa tctttcgcat tcgatgatac attttgcgaa agtgtgggca
    26341 aagatatatc agataatgtt gtgcgtactt ggattcatca acatactgtt attcttggta
    26401 ttcatgattc aagattgccg tttttaaaag atggcattga ttatttaacg aatgagattg
    26461 gttataatgc cattgttaga aattctggtg gcttaggtgt cgttctagat caaggtgtat
    26521 taaatatatc gctgatgttc aaaggacaaa cagaaacaac gattgatgaa gcgtttactg
    26581 tgatgtacct cttaattagc aaaatgttcg aaaatgagaa tgttgatatt gatacgatgg
    26641 aaattgaaca ttcttattgc ccaggaaaat ttgacttaag tatcgatggt aagaaatttg
    26701 caggcatatc gcaacgaaga gttagaggcg gtattgctgt acaaatttat ctttgtgttg
    26761 aaggctctgg ttcagaacgt gcattgatga tgcaaacatt ttatgaacat gctttaaaag
    26821 gtgaagtgac taaatttaaa tatcctgaaa ttgaaccatc ttgtatggcc tcattagaga
    26881 cattgcttaa caaaacgatt actgttcaag atgtaatgtt tttactatta tatgcaatca
    26941 aagatcttgg cggtgtatta aatatgacgc caattactca agaagaatgg caaagatacg
    27001 atacgtattt tgataaaatg attgaaagaa acaagaaaat gatagatcaa atgcaatagt
    27061 taaatattta acagcccctt tcaagttatc gtttggggct gttatttttg aaagtatcca
    27121 taaaaagcat aaatatgtta ttgacctaat atttaaattg aacatcatta aattaaaagt
    27181 aatgatatga tggaaataat aaatttaata agaaaatttc atgttttacg tattaaatag
    27241 attgagaata gggtatatat aatgcgtatg actatgaaag aaacgacctt ctattgaaat
    27301 gttctaatac tttagtagta aatctatata ttccaattat tatttgtagt attatagtta
    27361 gggtggaaaa ttataaattg tagggctatt ttataaaaag ataaagaatc tgaaatatca
    27421 ctcgataaga tttaaacttt gaagtaaacg aaagttagtt ttatactaat caagttaagt
    27481 caaatataaa ttcaaaataa agattataca taatatgaat aatgtgagat aaagactgca
    27541 atgaaattta acgagaatgc gtatgcaact tagaatatga tgtcttttac taataaataa
    27601 aaataatgaa tcaagtatag gataaatata aaaggtgata ttgacatgac aagaaaagga
    27661 tatggggaat cgacaggtaa gattatttta ataggagaac atgctgttac atttggagag
    27721 cctgctattg cagtaccgtt taacgcaggt aaaatcaaag ttttaataga agccttagag
    27781 agcgggaact attcgtctat taaaagcgat gtttacgatg gtatgttata tgatgcgcct
    27841 gaccatctta agtctttggt gaaccgtttt gtagaattaa ataatattac agagccgcta
    27901 gcagtaacga tccaaacgaa tttaccacca tcacgtggat taggatcgag tgcagctgtc
    27961 gcggttgctt ttgttcgtgc aagttatgat tttttaggga aatcattaac gaaagaagaa
    28021 ctcattgaaa aggctaattg ggcagagcaa attgcacatg gtaaaccaag tggtattgat
    28081 acgcaaacga ttgtatcagg caaaccagtt tggttccaaa aaggtcatgc tgaaacgttg
    28141 aaaacgttaa gtttagacgg ctatatggtt gttatagata ctggtgtgaa aggttcaaca
    28201 agacaagcag tagaagatgt tcataaactt tgtgaggacc ctcagtacat gtcacatgta
    28261 aaacatatcg gtaagttagt tttacgtgcg agtgatgtga ttgaacatca taactttgaa
    28321 gccttagcgg atatttttaa tgaatgtcat gcggatttaa aggcgttgac agttagtcat
    28381 gataaaatag aacaattaat gaaaattggt aaagaaaatg gtgcgattgc tggaaaactt
    28441 actggcgctg gtcgtggtgg aagtatgtta ttgcttgcca aagatttacc aacagcgaaa
    28501 aatattgtaa aagctgtaga aaaagctggt gcagcacata cttggattga gaatttagga
    28561 ggttaatgcg ttgattaaaa gtggcaaagc acgtgcacat acgaatattg cacttataaa
    28621 atattggggt aaaaaagatg aagcactaat cattccaatg aataatagca tatctgttac
    28681 attagaaaaa ttttacactg aaacgaaagt cacttttaac gaccagttaa cacaggatca
    28741 attttggttg aatggtgaaa aggttagtgg caaagaatta gagaaaattt caaaatatat
    28801 ggatattgtc agaaatagag ctggcatcga ttggtatgca gaaattgaaa gcgacaattt
    28861 tgtaccaaca gcagcagggt tggcttcatc ggcaagcgca tatgcagctt tagcagcagc
    28921 ttgtaatcaa gcgctagaca tgcagctgtc agataaggat ttatcgagat tggcgcgaat
    28981 tggttcgggt tctgcgtcgc gtagtattta tggtggattt gcagaatggg aaaaagggta
    29041 tagtgatgag acgtcatatg ccgttccact tgaatcgaat cattttgaag atgaccttgc
    29101 catgatattt gttgtgatta atcaacattc taaaaaggta cctagtcgat atggtatgtc
    29161 attgacacga aacacatcaa ggttttatca atattggtta gatcatattg atgaagattt
    29221 agctgaagca aaagcagcga ttcaagacaa agattttaaa cgccttggtg aagtaattga
    29281 agaaaatggt ttgcgtatgc atgccacgaa tctaggatca acaccgccgt tcacatatct
    29341 tgtgcaagaa agttatgatg tcatggcgct tgttcacgaa tgccgagaag cggggtatcc
    29401 gtgttatttt acaatggatg cgggacctaa tgtgaaaata cttgtagaaa agaaaaacaa
    29461 gcaacagatt atagataaat tattaacaca gtttgataat aaccaaatta ttgatagtga
    29521 cattattgcc acaggaattg aaataattga gtaaggaaga gataaaatga ttcaggtcaa
    29581 agcacccgga aaactttata ttgctggaga atatgctgta acagaaccag gatataaatc
    29641 tgtacttatt gcgttagatc gttttgtaac tgctactatt gaagaaacag accaatataa
    29701 aggtaccatt cattcaaaag cattacatca taacccagtt acatttagta gagatgaaga
    29761 tagtattgtc atttcagatc cacatgcagc aaaacaatta aattatgtgg tcacagctat
    29821 tgaaatattt gaacaatacg cgaaaagttg cgatatagcg atgaagcatt ttcatctgac
    29881 tattgatagt aatttagatg attcaaatgg tcataaatat ggattaggtt caagtgcagc
    29941 agtacttgtg tcagttataa aagtattaaa tgaattttat gatatgaagt tatctaattt
    30001 atacatttat aaactagcag tgattgcaaa tatgaagtta caaagtttaa gttcatgcgg
    30061 agatattgct gtgagtgtat atagtggatg gctagcgtat agtacttttg atcatgaatg
    30121 ggttaagcat caaattgaag atactacggt tgaagaagtt ttaatcaaaa actggcctgg
    30181 attgcacatc gaaccattac aagcacctga aaatatggaa gtacttatcg gttggactgg
    30241 ctcaccggcg tcatcaccac actttgttag cgaagtgaaa cgtttgaaat cagatccttc
    30301 attttacggt gacttcttag aagattcaca tcgttgtgtt gaaaaactta ttcatgcttt
    30361 taaaacaaat aacattaaag gtgtgcaaaa gatggtgcgt cagaatcgta caattattca
    30421 acgtatggat aaagaagcta cagttgatat agaaactgaa aagctaaaat atttgtgtga
    30481 tattgctgaa aagtatcacg gcgcatctaa aacatcaggc gctggtggtg gagactgtgg
    30541 tattacaatt atcaataaag atgtagataa agaaaaaatt tatgatgaat ggacaaaaca
    30601 tggtattaaa ccattaaaat ttaatattta tcatgggcaa taaatgattt gataggggag
    30661 caatcatcta atttaattag aagataaatg ctccattttt tattgtcagc cgtgttacgt
    30721 atcttgaaaa aaatattaaa aagagtaaaa tagatagagt taacgaaaaa attaatgaaa
    30781 tcgacgatat agatatgata aagaaaggtg ggtagcaata tgaaaaatac attccttatt
    30841 tgtgatgaat gtcaggcagt caatataaga acgttacaaa agaagttgga aaaattagat
    30901 cccgatgctg aaatcgtgat aggttgtcaa tcttattgtg gacctggacg ccgaaaaaca
    30961 ttcacttttg ttaataaccg cccactggct gcgcttactg aagaagaatt aatcgaaaaa
    31021 gtttctcaac aattaaagaa accacgtgat cctgaagaag aagagcgttt aagaaaacga
    31081 catgaagaac gtaaacgtcg taaagtagaa caagatagaa agcttaaaga aaaattagaa
    31141 aagcgaaaag cacaacaata aagcctgatg gcagcatcat tcaatgcgtg ccaccaggtt
    31201 tttatgtttt gtctagaaat taaataaatc attaaatgat tcggccatcg taggatgcgt
    31261 ataaatatta tctcgtaata cggtatatgg aatgttttga tcaatcgcaa gtttaattat
    31321 attaattaat tcttcagatt gcttaccata taatgtagca cctaaaatca tattattttc
    31381 attattaatg actactttaa ataaacctct tggatcattg ttaattttat gacgaggtat
    31441 agcacttact aaaagttgat gttcagtgta atcataatgt tgagcggcag cttctttact
    31501 agttaatcca acacgtgata atggtggatc tataaatact gtataaggca cgctgcctct
    31561 attgtcagtc gtacgtgact gattaccata taacgctgat ttgataattc gataatcatc
    31621 taaagatata tacgtaaatt gaagtccgcc tttaacatca cctgcagcat aaatatgcgg
    31681 cacagttgtt tgaagatgag cattgacttt aatttcgcct ctgtcgccta attcgatatc
    31741 agtattttct aaagctaaat ccgtattcgg tttgcgcccg atagccaaaa gtactgcatc
    31801 agcctcaaag ttatcaacgt tggtatggac tgttgtatga tgattgtcag atgacaattc
    31861 agtcgtttca acatttgtat gcaatgcaat gcctttattt tctaagtcag taataccata
    31921 tgcaacgaca tcttgatctt cgcgtggcat aaatgattcg ccacgttcta atactgttac
    31981 cttactacct aaattcgcaa acattgaagc aaattctaag gcgatataac cgccacctac
    32041 aataacgagg tgcttaggtt gatagctaat gtttaataaa cctgtcgaat cgaagacgtg
    32101 tttagcttga tcaaggcctt taatgttagg aatgacagag gtagcaccgg tattaataat
    32161 gatatgaggt gcagtaatac tatcgacgat atcgtcatgt tgatctaata aattcacttc
    32221 agtattagat ttaaactgcg ctttaaaatc cagtacatca atgttgttat cgtctgctaa
    32281 taagtggtaa tttttattgt ttagcgcatt gacaacatcg tttttacggt tataacttgc
    32341 ttcaaaagat ttgccttcta atccatcatg tacaagtgtc ttcgaaggta tacatcctat
    32401 gtttatacaa gtgcctccat acattttcgg agattgttcg ataactgcga cgtgttgacc
    32461 tgttgatgca gcgtatttcg ctaaagtttt accagctttc ccaaatccta ttacaattaa
    32521 atcatatgtt ttcatgacat aaatcctcct tttgaatgtc ttcaatgaca tctttgattg
    32581 tttttccatt ataaaaatta ataatgatat tctgttcgtc ttgctgataa tgtgacatgg
    32641 tagttgcaat attacgagca atttgacagt gactgccttc gtcgccagta aatagacgtg
    32701 tgtggtgttc tttctctaag acaaaatgtt tatataatgt tgctagagag acatcagcac
    32761 tttgatcatt tgctaaataa ccgccatctt tacctcgtat tgtgtcaatc atttttaaat
    32821 cgacaagttg agtcgtcacg cgtcgtaatt gaacaggatt taaacaagtt aattctgcta
    32881 atgaactact attgaatttt tctgaatgat gcttagttaa aaaagctaat acatgcacgg
    32941 caatgttaaa ttctaaattc aatgtttctc acctcataat tgtaactgta ttagttacaa
    33001 ttaaaaatca ttttgctcaa tgtgtcaata cttatgtttg aaaagtgttg aattaggtgt
    33061 ggttattgtg atgatttatt gtttgaaagt tgatgcatta acggtgagat tgcacaattg
    33121 aaattttaat tatgtatgaa ctgatagata tgaaatgaat attgaaaatg agagcaataa
    33181 aattttgaat gatgatgttg tgtgattgta taaacttatt cgtattttag atacgttttg
    33241 atatttcgca tgatgaggaa atacttttaa caactgatga catattttaa tagtgaatat
    33301 attttgagat attaatatat ttaatgttaa ggatgttgtc gcaataaaat taaaatattg
    33361 aaagataatt tttattgatt aattgcgaat atacaaagac accattatat ccatatgcta
    33421 aaataagaaa aaaggcgaca tgttatctca atttacctgc atctagactc ctttacataa
    33481 gaaataatca tcgccgagtt ttaaaatttt acaactttgt agatgtaaaa agcatttgat
    33541 tttaaatagt ttgaaaatca tatatttttg aaaaaggagc atgccgatga gtattgacat
    33601 gtatttagac agatctcgaa accaagcttc aagtgtgggg aatttgagtc aaacaatgaa
    33661 ttcaaattat gatgcgttgg aaaaagcaat tactcaattt attaatgatg atgcgcttaa
    33721 agggaaagcg tatacgtcag ctaagcaatt ttttagtacg gtgttaattc cattatcaac
    33781 aagtatgaaa acattgagtg atttaacgaa gcaagcttgc gataattttg tgtcacgtta
    33841 tacgagtgag gttgatagca tatctttaaa agaatcagag cttgaagaag atatcagatc
    33901 attaagtcaa caaattacgc gatatgaaaa tttgaataac aatttgaaaa agcatgcttc
    33961 cgataatcag caagccattt catcgaacca acaaataata cgaacattag gtcaacaaaa
    34021 acatgaatta gaagagaagc tacgcaaatt gcgtgagttt aatcaaaaat caccagaaat
    34081 atttaaagaa gttgaagaat ttcaaaaaat tgtccaacaa ggacttaccc aagcgcagaa
    34141 tttttggaac ttttcaacaa atcaatttaa tattccttca ggtaaagaac ttgattgggc
    34201 caaagcaagt catgaaaaat atttgaaagt tgctatgggg aaaattgaac ataaagcaga
    34261 gaaagaaact ttaaataaag cagactttgc tgttataaag gcatatgcca aagaacatcc
    34321 agaagacgat atcccgaaaa gtatattgaa atatataaat gacaataaag acagtattaa
    34381 aagagatata ggattagata ttacgtcaac acttttagag caaggcggta taaatgcaag
    34441 taaattcggt gtatttatca atacagcagg tggagtgaaa ggcccagcag gtccaaattc
    34501 atttgtggaa gtcaaacgta catcaggtaa tgtgtttata gaaaatggta gtaaatttgc
    34561 aaaaggcgga aaatacctag gtaaaggtgt tgctggtgta ggatttggta taggtatgta
    34621 tgatgacctt gcaaatgatg ataaaacatt tggagaggcg ttgtcgcata atggtatgac
    34681 gcttgcagct ggatctgcag ggacagcagt tggagctgga ttagcgactt ttgttttagg
    34741 aagtaatcca gtaggatggg tgattttagc aggtttggct atgagtacag tatttgcatt
    34801 aggaacagat ttaatttatc aaaataatat ttttggatta aaagataaag tagactgggt
    34861 agggcataaa atcgataata gtatagatgt tgttaaaaaa actacagaaa aatccatgga
    34921 tagtgttggg aatgctgtta gtgaagctaa aaatattatt agcaatcata taaatccaat
    34981 gaaatgggcg tggtgagttt tgctttttaa tattaaggtt attaataaaa atccgagata
    35041 taaagtcgtt caatataatg atgagtattt gttaattgat ttagtaagca cttggcttgt
    35101 atactttttt ccttttatta attggttcat tcccaaaagg tatgcaaaac ttagtgagaa
    35161 ggaacttgaa aatttaaatg ttgataaaca aaataaaaat aatattttct ggccagttac
    35221 tggtagttcg tttttatttg tagttatttt acggaaatat gtacatacgt ttgaagttca
    35281 attagataat aagattttaa tatctttatg ttttatagga tttataggta ttgcagcatt
    35341 ttacatttac ttaaataaaa agcttaaact aaagatatat gatgataatc tagataatga
    35401 aaatagggtt atattagtgc ctacttttaa agatggaagt tttatagttt tcacatatct
    35461 attattagga ggttgttcta tattattttt aatatggcta atgactataa aacctcaaaa
    35521 tctactggta tttattatgt ggattattat tacaattttt ttctttctaa taagcatggg
    35581 ctcaattagt aataaaaagg tttatgccaa acttaaaaag caatagaaat tactaaactt
    35641 agattgtagt tcgtaagtta agtaataaac aggaaaatag tatgttacta aaatacaaaa
    35701 cacttataaa gtatctgaaa gtgtattaga tataaaacca acgagacgga agtggagata
    35761 ttgctttgcg aggttagagt tgtaaacaaa aatcccagat atagaattat taaatataaa
    35821 aacgactatc tgatgattga tttagtaagc acctggttag ttttgttctt tccgtttatt
    35881 aattggttaa taccaaaaaa atatgtccaa atcagcagag aagattttga taatttaaat
    35941 attgtcaaac cagttaaaaa taaagcttta tggccagcta taggtagcat acttttattt
    36001 ggcactatgt ttagagataa aatatatata cctgattctc atttagaaaa aaattgcgtt
    36061 attatcattt gttcggtttt attattaagt attctagtgt tttatatata tttaaatcag
    36121 aaagtaaagt tatctatcta taatgatcgg agtagcaatg gaaaaattat gatttttcca
    36181 tcattcaaga atctttgctt tgtactattt tcttattttt tctgtggtgg attatcaatc
    36241 atgttcttag atgttttgat tagtttatct atccaaaata ttatagtatt tattgcttgg
    36301 gttattatga caatgctatt tttctttata aatatgtctt caataataga caaaaagatt
    36361 catgttatat atttaaggtc atataaatat taattttgat acaaaagggc acaagtgttt
    36421 tgagaaacag aaacgataat atagctgtgt gaatttgtta ttaataggaa gtgataatta
    36481 ttgctttgtg aaaccgaaat tatcaataaa aatccgaaat acagagttat taagtatgat
    36541 gatgagtatt taatggtcga tgtaataaga acttggcttg tatacttttt tccatttatt
    36601 aattggttca ttccaaaaag gtgcgccaaa attagtagag aagagtatga aaaactgaac
    36661 actgttaagc cagttaaaaa taaaaatttt tggcctgttg taggaggaac aatcctacta
    36721 ggtgctactt ccagaaagta catacactta cttaatattc aattagaaaa aagatcggtt
    36781 atatttatat gcttcgttgt atttctatgt attttaatct tttttgtgct tctgaatcga
    36841 aaattaaaat tgaaagtttt cgataataaa aaagaagaac aaaaaataat tttagtacca
    36901 acattaaaaa atgctgttct tatattatat ggctatttgt tgattggagg catgtcaata
    36961 ttagctttaa gcatgttgct gacattagaa aaccagaatt taataacttt tatagcttgg
    37021 ggtatggggt taatgttgtt tttcttgatg aatataacac tcattgtcaa taaaacagtc
    37081 aaagttataa aaaggtgaat aaataactaa ttatattgta ttggccgttc ttcaaataaa
    37141 aaataatttg aataaggtgg aagtgataaa cagtgctttg cgaatctaga gtcattaatc
    37201 aaaaccctaa atatagaatt attaaataca ataatgaata tttcatggtt gatttagtaa
    37261 gtacttggat tgcttatttt ttgcccatga ttaattggtt tattcccaaa aagtacgcga
    37321 aaattagtag agaagaattt gaaagtttaa atattgtcaa acccgctaaa aataatactt
    37381 tctggcctgt tgcaggattt gcagtgttat taacaacctt aacaagaaaa tatatctatt
    37441 tgcttaacat ccatttagaa aaagaaatag ttatattaac atgctgtatg atacttctag
    37501 gtgttttcgc attgtttata tatataaata caaaattgaa gttacatatt tttgataaaa
    37561 ataaaagtaa taacgaaaag atcatattaa tacctacatt taaaaatatt tgtttatcct
    37621 tatttgctta tatattattt ggtggattgt caacaatggc tctgagtatg ttagtaactt
    37681 catcccctca aaatataata gaatttcttg ctttaattgg catgactgca tgcttctttc
    37741 tactgaatat gtcatcggtt ctagataaaa aaattcatgt tattttaaaa acaaataagt
    37801 agtaaaattg attaacttag gtagtatcgg atacttaaat gttggttcat aaaaagcaat
    37861 gattttaaat cgaggagcta tcttagaaca gggaaataaa acagccaaag ttataaaaag
    37921 tgaattaata actaattata ttatgttagc cacgcttcaa ataaaaaata attagaataa
    37981 ggtgggattg ataatcaatg ctatgcgaat ctaaaatcat caataaaaac cccaaatata
    38041 gaattattaa atataatgat gagtacttaa tgatcgatat aataagcact tggattagtt
    38101 tattttttcc ttttattaat tggttcatcc ccaaaagata cgtcaaaatt agtagagaag
    38161 agtttgaaaa tttaaatatt gtgaaacctg ctaaaaagaa tgttttttgg ccagttgcag
    38221 gtatctctac tttattcgca gttacattaa gaaagtatac acatttactt gacactcaac
    38281 ttgatagaaa attagttatt gctatatgtt gtatcacatt tatagggatt ttaacatttt
    38341 atgtacgcct a
//
LOCUS       DALAAR010000021        37006 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000021 DALAAR010000000
VERSION     DALAAR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.021"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..173
                     /locus_tag="O2458_001079"
     CDS             <1..173
                     /locus_tag="O2458_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3886952.1"
                     /translation="IVRSVNRLNNKGMLIKERSHADERIVLIKINKIQYNTIKSIFTD
                     TSKILKPRKFFF"
     gene            complement(384..1250)
                     /gene="galU"
                     /locus_tag="O2458_001080"
     CDS             complement(384..1250)
                     /gene="galU"
                     /locus_tag="O2458_001080"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCZ3886953.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1430..4252)
                     /gene="fnbB"
                     /locus_tag="O2458_001081"
     CDS             complement(1430..4252)
                     /gene="fnbB"
                     /locus_tag="O2458_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCZ3886954.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(4934..7990)
                     /gene="fnbA"
                     /locus_tag="O2458_001082"
     CDS             complement(4934..7990)
                     /gene="fnbA"
                     /locus_tag="O2458_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCZ3886955.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(7974..8075)
                     /locus_tag="O2458_001083"
     CDS             complement(7974..8075)
                     /locus_tag="O2458_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886956.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(8459..9817)
                     /locus_tag="O2458_001084"
     CDS             complement(8459..9817)
                     /locus_tag="O2458_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCZ3886957.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(9934..11487)
                     /gene="gntK"
                     /locus_tag="O2458_001085"
     CDS             complement(9934..11487)
                     /gene="gntK"
                     /locus_tag="O2458_001085"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCZ3886958.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(11511..12191)
                     /locus_tag="O2458_001086"
     CDS             complement(11511..12191)
                     /locus_tag="O2458_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3886959.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(12345..13109)
                     /locus_tag="O2458_001087"
     CDS             complement(12345..13109)
                     /locus_tag="O2458_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ3886960.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(13274..13966)
                     /locus_tag="O2458_001088"
     CDS             complement(13274..13966)
                     /locus_tag="O2458_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ3886961.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(14197..14613)
                     /locus_tag="O2458_001089"
     CDS             complement(14197..14613)
                     /locus_tag="O2458_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCZ3886962.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(14752..16575)
                     /locus_tag="O2458_001090"
     CDS             complement(14752..16575)
                     /locus_tag="O2458_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCZ3886963.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(17053..18330)
                     /locus_tag="O2458_001091"
     CDS             complement(17053..18330)
                     /locus_tag="O2458_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3886964.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            18877..19488
                     /locus_tag="O2458_001092"
     CDS             18877..19488
                     /locus_tag="O2458_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCZ3886965.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(19497..19595)
                     /locus_tag="O2458_001093"
     CDS             complement(19497..19595)
                     /locus_tag="O2458_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886966.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(19585..20748)
                     /locus_tag="O2458_001094"
     CDS             complement(19585..20748)
                     /locus_tag="O2458_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3886967.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            complement(20741..21199)
                     /locus_tag="O2458_001095"
     CDS             complement(20741..21199)
                     /locus_tag="O2458_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3886968.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            21633..22328
                     /locus_tag="O2458_001096"
     CDS             21633..22328
                     /locus_tag="O2458_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3886969.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            22330..23100
                     /locus_tag="O2458_001097"
     CDS             22330..23100
                     /locus_tag="O2458_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3886970.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            23473..25437
                     /locus_tag="O2458_001098"
     CDS             23473..25437
                     /locus_tag="O2458_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCZ3886971.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            25781..26860
                     /locus_tag="O2458_001099"
     CDS             25781..26860
                     /locus_tag="O2458_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886972.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(26965..27558)
                     /locus_tag="O2458_001100"
     CDS             complement(26965..27558)
                     /locus_tag="O2458_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3886973.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(27580..28548)
                     /locus_tag="O2458_001101"
     CDS             complement(27580..28548)
                     /locus_tag="O2458_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCZ3886974.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(28626..29060)
                     /locus_tag="O2458_001102"
     CDS             complement(28626..29060)
                     /locus_tag="O2458_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3886975.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            29287..29571
                     /locus_tag="O2458_001103"
     CDS             29287..29571
                     /locus_tag="O2458_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3886976.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            29742..30548
                     /locus_tag="O2458_001104"
     CDS             29742..30548
                     /locus_tag="O2458_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3886977.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(30668..31339)
                     /locus_tag="O2458_001105"
     CDS             complement(30668..31339)
                     /locus_tag="O2458_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ3886978.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            31609..32601
                     /locus_tag="O2458_001106"
     CDS             31609..32601
                     /locus_tag="O2458_001106"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ3886979.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(32850..33650)
                     /locus_tag="O2458_001107"
     CDS             complement(32850..33650)
                     /locus_tag="O2458_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3886980.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            33971..34606
                     /locus_tag="O2458_001108"
     CDS             33971..34606
                     /locus_tag="O2458_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3886981.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            34612..35343
                     /locus_tag="O2458_001109"
     CDS             34612..35343
                     /locus_tag="O2458_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCZ3886982.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(35503..36123)
                     /gene="srtA"
                     /locus_tag="O2458_001110"
     CDS             complement(35503..36123)
                     /gene="srtA"
                     /locus_tag="O2458_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCZ3886983.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            36317..36808
                     /locus_tag="O2458_001111"
     CDS             36317..36808
                     /locus_tag="O2458_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3886984.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
BASE COUNT        11507 a         6808 c         5444 g        13247 t
ORIGIN      
        1 aaattgttag gtcagttaat agattaaata ataaaggtat gctaatcaaa gaacgatccc
       61 atgcagatga aaggattgtg ttaatcaaaa taaataaaat acaatataac actattaaaa
      121 gcatattcac agatacttcc aagattctca aaccaagaaa atttttcttt taaatttaaa
      181 cagatttacc tcttgataaa ataaataagc aatcatacta cttctcaatt tagtataaat
      241 aaaaatacat aattaacttt cttttgtttt tatattattt caatacccta ctatatatca
      301 caacacataa attaagcatg acactcattc aatttagttc accatttcgt gttccaattt
      361 tactgagtat catgctttta atgttataaa cctaatgctt taataaatcg tgttaattct
      421 tctcgcatac tgtcatcttt caatgcatat tctatggtag ttttaacgaa gcctaatttt
      481 tctccaacgt cataacgttc gccttcgaag tcatatgcat acacttggtt atcattattc
      541 atacgttcaa tcgcatctgt taactgaatt tcgttacctg cgccttcttt ttgcgttttt
      601 aaataatcga aaatttcagg cgttaataca taacgtccca taatagctag gtttgatggt
      661 gccgtacctt gtgctggctt ttcaacaaac tttttcactt catactgacg tccgttttta
      721 gttaatgggt caataattcc ataacgatga gtatctgctt ccggaacttc ttggacacct
      781 ataactgagt gccctgtttc ttcataaacg tcaatcaact gtttcactgc tggcacttca
      841 gattcaacaa tatcgtcacc taataatacc gcaaatggtt cattgccgat aaattgacgc
      901 gcagaactaa tcgcatgccc taaacctttt tgttctttct gccttacata aaaaatattc
      961 gcaagttccg ttgaatactg aactttctct agtaattcag atttaccttt ttcttttaac
     1021 accatttcta attctttttg actatcaaaa tgatcttcaa tcgcgcgttt gtggcgacct
     1081 gtcactataa taatatcttc aattccagct cttgcagctt cttcaacgat atattgtatt
     1141 gtgggtttat ctaagatagg aagcatttcc tttggcatcg ctttagttgc tggtaaaaat
     1201 ctagtcccta aaccagcagc gggaatgatt gcctttttta tttttttcaa agttaatgtg
     1261 ctccttttcc taagtattaa atctatgtat caacgtcatt ttaacactaa ttagaacgcc
     1321 ttcatagtgt cattgagtat gtaattattt cttgggaaat ttgttttaat tttaaaaaac
     1381 aggcttactt catataattt atgaaataaa cctgtcaatt ttggattgat tatgctttgt
     1441 gattcttttt atttctgcgt aataacgcta aacctaaaat gctaaataat ccgccgaaca
     1501 acatgccgtt gtttgttgat tcttctccac ctgtttcagg tagttcagat ttcttagatt
     1561 gtgctttttt agttggtacc actgctttaa ccttttcatt gatttcaata acaggtgtta
     1621 ctactttacc ttgttccact ggtttagaag gttttttagg ttcttcttta gcaggtggta
     1681 ttggtttacc aggttcagtt ggtacctctg gcgttggcgg tgttggtgtt tccggctcgc
     1741 ttggtacttc tggtgtcggt ggtgttggtg tttccggctc gcttggtact tctggtgtcg
     1801 gtggcgttgg tggcacgatt ggaggtgttg tatcttcttc aatcgtttgt tgaccttcat
     1861 tatgaccact tacttgtgga agtgtatctt cttcaaagtc aacactattg tgtccaccga
     1921 attgataatt tggtttatct ttatttgtat cttcttcaat aatttcagtg tgcttattga
     1981 atccgtgaat atgtggcaca ctgtcgaagt cgatatcaat gatattacca ccttgttcat
     2041 acttaggttt gtctttctct gtatcttctt cgaatgattg gttaccatta ttttgaccat
     2101 gaatttgagg tacactatcg aaatcgatat ctacgatatt gccaccttgt tcatatttcg
     2161 gtttatcttc ttctgtgtct tcctcaaatg actgattacc gctattttgg ccaccttcgt
     2221 aacctaattc actcttaata tccacgtggc tattttcttc gatttcttca atcacgccat
     2281 aattaccgtg accattttca gttcctaaac cagaatgaga aatatgatga ttgttttcag
     2341 taatttcctc gattggtcct tgcgcttgac catgttcttc aggtagttca tctactagtt
     2401 caatcagatt actttcagtc gtatattctt tcgtatcttc aattgttgta tgatcgctaa
     2461 cagcaccagt tacaatacct tttgtagaat cttcgtcaaa ttcaactagg ttagactcag
     2521 tagtaacctg accaccacct gggtttgtat cttcttcata ttcaacaaca tcagcatgat
     2581 gttttgaatt ttcatgtgtc gattcttcaa agtctacatg aatagaatct tcttcagttt
     2641 caatggtacc ttctgcatga ccttctgcac cttcaacagc tgtatgatat tcaaaatcaa
     2701 ttggcttaga atcattactt tcttcgattg taccagtcaa ttcatgcttc tccactggcg
     2761 gctctgattt aaattcaagt tcgataggag tactatgttc tataataggt tcctttagtt
     2821 tatctttgcc gtcgccttga gcgttattag agtaaaatgc aacgccattt ttccaagtta
     2881 aattacttgt ataataatag ttataatatc caaaaaggtg tgtttgaaat tctaagttgc
     2941 tagcatttga atcataatac ccttcatatt ttattacata atttttactt tggtctaaat
     3001 tattaaagtt taaagaataa ccaccattag tatcaaaatc taaactcata ttatcagtca
     3061 catcttcaaa tttgctgaca tcatcaagct ttgcatatac gctttcagct aaatcgtctg
     3121 aaccaatgtg tttatatacc ttaactgttg gattattaac ccctggttta tttcctttag
     3181 ttacttgacc agttactgtc acagagctta acgactggtt gttaggtttc atgtacgcaa
     3241 aatgactaaa tttcccatct actttattta aagtatcaat tcgaccatta gctgttactc
     3301 cccaattatc tctaactcca cctaaatatt gaatattaaa tattttgcta accgtagtct
     3361 cacccaattt aacttcaaca ttttggttac ctttttgcgt cactgttgta ggatcaataa
     3421 atagatttaa agataattca gcagttaaat ctttcttttc ttgtacatat tctttaaacg
     3481 tatatctaac ttttctttct ccaattattt ctcctgtcgc cataacttga ccatctgtac
     3541 tttttatctc cggaacttta cgcagtgttg agataccatg agtttcaaca ttatcgctta
     3601 atgtgaaatc aaaataatct cccgccttaa ttccttctcc aaatttccat ttatatttca
     3661 aggttactct ttctgcgtta tgaggattta caacattcgt atcttgttta tgtcctacaa
     3721 tttcactacc ttcttctact tccactttat ttgttacatc tgtacctgtc gctttagttt
     3781 cttccactac ttctttctct gcaactgctg taacgtcagt tgatcttttc attcttggtt
     3841 taatttctga gacgttactt ggttgagcta tgtcaacttg agttcctgta gtttccttat
     3901 cagcaacttt ttccgatggc aaatcaactc gcgaagtttc tacttttggt gcttgcacag
     3961 ttttcggtgc ttcttctgtt gttacttgtg ttgattgtga tggttgctca gttgatgtcg
     4021 cgctgtatga ttgtgtttca tctattgtat taacgttatt tgtagttgtt tgtgtttcgc
     4081 ttgctttact ttcagtagct gaactcccac tttcctctac tgtagtattg ttttgttccg
     4141 atgctgcagc ttctttttct tgtcccattc caacaacgat cattgttcct aagaatactg
     4201 aggccgctcc caatttgtgt tttcttatgc cgtatctaag attgcttttc actataatat
     4261 tctcccttaa atgcaaaatt catttatttt taaaactcaa taaatgcaat tctatattgt
     4321 tcggttttta aaagcaatga aaaaaagcga gttaataaaa agttaagatt gttgttaact
     4381 ttatgtataa tgagtttttt attatttgaa actcacatat atattgcata caaagctctt
     4441 gaacaccttg atataacagg cttgtatttt ttatacttac tttttaaatt aaattcaaat
     4501 tcatctaatt taaaacaata tactaaacca tacataataa tcgcctgtac aatgcatcat
     4561 taacaagtca ctgaaacgcc tttcattgta ttaataacgt cactataatt tttatatcgt
     4621 tcggtttttg tttgatttta atgattattt atacaaaaac agccgtattt caagccgaca
     4681 ttttaaattt aactaaattt gcatctagtt aataattgca tttatcaaat ttgtcttatt
     4741 gatccaatct aatttgtact cacaaactag tttaaaattc taactttatc tctcagttcg
     4801 ttatcaatca tcagacataa accaatgaag caatcagaaa acactctaat tttctattag
     4861 aaatttgatt taatataaaa aaacaggctt acttcatata atttatgaaa taaacccgtc
     4921 aatttttgtt taattatgct ttgtgattct ttttatttct gcgtaataat gctaaaccta
     4981 gaatgctgaa taatccgccg aacaacatac ctttgtttgt tgattcttct ccacctgttt
     5041 caggtagttc agatttctta gattgtggtt ttttagttgg tgccactgct ttaacctttt
     5101 cattgatttc aataacaggt gttactactt taccttgttc cactggttta gaaggctttt
     5161 taggttcttc tttggcaggt ggtactggtt taccaggttc agctggtacc tctggtgttg
     5221 gcggtgttgg agtttctggc tcactcggca cttctggtgt cggtggtgtt ggtgtttccg
     5281 gctcacttgg tacttctggt gttggtggcg ttggtgtttc cggctcactt ggtacttctg
     5341 gtgtcggtgg cgttggtggc acgattggag gtgttgtatc ttcttcaatc gtttgttgac
     5401 cttcattttg gccgcttact tttggaagtg tatcttcttc aaagtcaaca ctattgtgtc
     5461 caccgaattg ataacttggt ttatctttat ttgtatcttc ttcaataatt tcagtgtgct
     5521 tattgaatcc gtgaatatgt ggcacactgt cgaagtcgat atcaatgatg ttaccgccat
     5581 gttcatactt aggtttgtct ttttctgtat cttcctcgaa tgactgatta cctttatttt
     5641 gaccatgaat ttgaggtaca ctatcaaaat cgatatctac gatattgcca ccttgttcat
     5701 atttaggttt gtcttcttct gtgtcttcct cgaatgactg gttaccgcta ttttggccac
     5761 cttcataacc taattcactc ttaatatcaa cgtggctatt ttcttcgatt tcttcaatca
     5821 cgtcataatt cccgtgacca ttttcagttc ctaaaccaga atgagaaata tgatgattgt
     5881 tttcagtaat ttcctcgact ggtccttgtg cttgaccatg ctcttcaggt aattcatcca
     5941 ctaattcaat cagattactt tcagttgtat attctttcgt atcttcaact gttgtatgat
     6001 cgctcactgc gccagttaca ataccttttg tagactcttc gtcaaattca actaagttag
     6061 actcagtagt aacctgacca ccacctgggt ttgtatcttc ttcatattca acaacatcag
     6121 cgtgatgttt tgaattttca tgtgtagatt cttcaaagtc aattggattt gattcctcag
     6181 aggactcagt gtatcctcca acgtgacctg cttcgctatc cacagcagta tggtaatcga
     6241 tatcaatagc tgatgaatcc gtttcttcta ttgtttcaat gtatccatca acatatccac
     6301 ctccaccatc tatagctgtg tggtaatcaa tgtcaagagt tgatgaatca tattcctctt
     6361 caacagtagt tactaaattc ttatcatatt gacctgtaag agtttcttta attgtatctt
     6421 ctttatattc aaatttatta ttttgaataa tcggaccatt tttcccattt ccgttcgctt
     6481 tattactgta taaaactaaa ccattatccc aagttaaggt atatcctcta tcataataat
     6541 acttataaag ttgctctgga tgtcctacca tttgtgttct aaaatcaact tcatcagtac
     6601 catttaaata ctctccatca tagtgaacaa cataagtttt atctagattt tctatattca
     6661 atgaatagct tccattattt tgtaaattca aattcccact catattactt gtgacttctt
     6721 taaatttaga agtatctgtc gtatttgcat atacactctt cgctatgtct tcattattac
     6781 ccaagtattc aaatatccta acttttggtt gatttccatt ctgattacta cctttcatta
     6841 aagttccagt aacagtcaca cttgtcgttt taccattatt aggtttaata aatgcaacat
     6901 gcgaaaatct attattcgct ttattaaatg tctcaatcga tccatttaaa ttggcataat
     6961 aattcccaat accatcttta tatttaacat ctaattcctt tgaagtttgt tcttcattta
     7021 gtgttgaagt tatagtttga tttccattag tttgtacagt tttaggatca ataaataaat
     7081 taatttctag ttcagccgtt acatcaacct tatcttcaat atcatttgta aatgtatatc
     7141 taatctttcc accttctaaa acttcacctg tcgccattac gactgaacca tttttaattt
     7201 ctggtacttt tctagcagtt gatacgccat gcgtatttac attatttgat aaagtaaagt
     7261 caaagtagtc accttgatgt aaaccattct caaatttcaa cttatatttt agtaccgctc
     7321 gttgtcctgc atgaggttct actttatttg tattgttatg cccctcaata gaaccaattt
     7381 ctactgtaac tttacttgtt acatctgtac ccgtttccac tttcgcgtta ctagcttcct
     7441 tagcttccgc tacatctgct gatcttgtca cacgtggctt actttctgat gccgttcttg
     7501 gctgtgccac ttcaacttgt gtttctgcga cttgattttg tgtagccttt ttaggtgtta
     7561 aatctacttg tctttgatct ccgctattgt cttgagattg tgttgtttcc ttaacttgag
     7621 gtttcgcttc ttccttaact acctcttctt taactgtttc tatatttgct ggttgtgcag
     7681 tttgtggtgc ttgtactgct tttggtgctt cttcagttgt tacttgtgtt gcgtttgacg
     7741 gttgttctgt tactgttgcg ttatatgatt gagtttcttc tatatgatta acgttagttg
     7801 cagttgtttg tgtttcactt gttttattat cagtagctga attcccattt tcttctactg
     7861 tagttgtctt ttgttctgat gctgcagctt ctttgtcttg tcccatccca acaacgatca
     7921 ttgttcctaa gaatactgat gctgctccca atttatgttt tctaatgccg tacctaagat
     7981 tgtttttcac tataatatct ccctttaaat gcaaaattca ttaatttttt aaacttaata
     8041 aatgcaagtc tatattgttc ggtttttaaa agcaatacaa aaaagcgagt taatgaaaag
     8101 ttaagattgt cattaatttt atgttaattg ataattttat tatttgaaat atacctataa
     8161 attgtattca agtcatcaga aacccttgtc acacaaggct tgtatttttt atacttattt
     8221 ttttaaatta aattcatcat tatctaattt aaaacaatat actaaacgtt tcataattat
     8281 cgcctgtaca atacgcacaa aaacatgtct tgaaacgcct ttcatcactc taaaataccc
     8341 aatatacttt ttatatcgtt cggattctga gtatttcaga cgattttctg cataaaaata
     8401 aacgtgtttc aaggcaatat attgcaatta cctaaaaaca cgtttactta atatttagtt
     8461 aaacaaataa gctaatgaat aaaatgaaga tgatacctga aacggaaata atcgtttcta
     8521 ataatgacca tgttaagaat gtttctttta cagttaaacc aaaatattct ttaaacatcc
     8581 aaaatcctgc gtcatttaca tgagacaaaa tcacactacc tgcacctatc gcaagtacaa
     8641 ctaatgcaac atttacatct gatgattgta ataatggtaa gacaatacct gtagttgaaa
     8701 tcgcagctac tgtagccgaa cctaatgcga tacgtagcac agctgcaaca atccatgcta
     8761 gtaaaatcgg agacatctct gtaccttcaa acattttagc aattgtattt ccgacaccgc
     8821 cgtcaattaa tacttgttta aatgtaccgc caccgccaat aatcaataac atcattccga
     8881 ttggataaat cgcattcgtc actgattcca taatatgatt catcttacgc tttctcatta
     8941 atcccatcgt aacgattgca aataatactg ctattagcat ggctgtccct gctgttccta
     9001 tcatataaat gatagattca aatagatttg taggtttgtc atgcccagtt acaagttgcg
     9061 ttatcgtaga cactaacatt aatatgactg gtaatgttgc tgttaataaa ctcataccaa
     9121 atcctggcat ctcttgatcc gtaaattctt tttgtgcacc taacgctgaa atatcgcctt
     9181 ctcgtgtata cgcagacgga atcatttttt gtgcaacttt gttaaatata ggccctgcaa
     9241 tgagtgtaac tggaatggca ataatcatac catacagtaa tacatctcca acatttgcct
     9301 ttaattcttt tgcgatgact accggtcctg gatgtggtgg taaaaagcca tgtgtcactg
     9361 ataaagctgt taccataggt agtcctagtt ttaacactga aacatttgcg cgttttgcta
     9421 ctgtaaatac taatggaatc agtaagacta aacctacttc aaagaacaat gcaataccga
     9481 cgataaatgc tgcaacaagc attgcccatt gtacatgttt ttgaccaaat ttttgaatca
     9541 acgtgtctgc gattcgagtt gcaccaccac catcagcaag caatttccca agtatggcac
     9601 ctaaaccgaa tatcagtgca atgtggccga gcgtactgcc cattcctttc tcaatcgtct
     9661 ccataatttt agtcaatggt atacctagca ttaacgctgt aatcatcgat gtgataatta
     9721 atgaaataaa tgtatttaat ttaaacccaa taattaatac taataaaata acgataccta
     9781 aaacaacact gattaacggc catatttcgt taaacatgac attcccctct ttctcttttc
     9841 aatagaatgt aacaccgtcg tcgcgcacgt attccacata atatatagta gtcatggcac
     9901 aacaacttta tctaagatgc ttgagtgacg tatttattgt gttttatttt cagcgatatg
     9961 ttggcgttga aaatctgcaa tttgttcata attctctgtt aaagaacgac ttaaattgat
    10021 aaaaatggat acgatctctt ggtaaacagt gacattttct tcaatcggcg tatgattgtt
    10081 tgtggcaccg accatcgatg aaacgattga aaaatcttca atgtcaccta cagctttaag
    10141 tccgagcacg caggcaccta agcatgaact ttcataactt tcaggaacca ctaactctgt
    10201 gtcaaatata tctgacatca tttgacgcca tacttcactt ttcgcaaaac cacctgttgc
    10261 ttttatcatc ttaggtgttt cattcattac ttcaataagc gcaagataga cggtatacaa
    10321 attgtaaaga acaccttcta atgcagcgcg aatcatatgt tcttttttat gagataaagt
    10381 taaaccgaag aatgaacctc ttgcatttgc gttccaaagc ggcgcacgtt ctcctgctaa
    10441 atagggatgg aatattaaac catctgcacc tggtttaaca cgctttgcaa tttgagttaa
    10501 gacatcataa ggatcaacac cgagacgttt cgcagtttcg acttcactcg ctagcaactc
    10561 gtcgcgcaac catctcaata cgacaccacc attatttaca ggacctccga tgacgtagtg
    10621 gtcctctgtt aagacataac aaaatattct acctttgtaa tcagtacgcg gtttatctat
    10681 cacagtacga atcgccccag atgtaccgat tgtgacagca acttctcctt taccaacact
    10741 attgacacct aaattagaaa ggaccccatc actcgcacca ataacaaacg gtgtatcttt
    10801 attaagcccc attaatgttg cataacgttc tttcatacct ttcatcacat acgttgttgg
    10861 aactaattcc ggcaacattt ccttggaaat acccagcagt tctaatgcct caacatccca
    10921 atctaatgtt tctaaattaa acatccctgt tgcggaagcc attgaataat caatgatata
    10981 tgtatcaaat aaatgataga aaatgtatgt tttaatatct gcaaacttag cagtacgttg
    11041 aaatacatct tgccattcat gtttcatcca aaaaatcttc gctaatggcg acataggatg
    11101 aatcggtgtg cctgttcgct ggtaaatcgc attgccatca tgcacttcat ttattactgt
    11161 tgcatatttt gcagcgcggt tatctgccca agtaatatta tttgttaatc tttgatgttg
    11221 ctgatccatc gcaatcaagc tatgcatttg cgcactaaat gacacaaact taatgtcgtc
    11281 tttattaact ttggattctc tcataacata tttaatagtc attagtactg catcaaataa
    11341 ttcatctggg ttttcttctg agacatcaac gtttggtgtg tgtaaatcat agcctatttg
    11401 atgtttcatg ataaaagttc cattttcatc atataagact gacttggtac tcgtcgttcc
    11461 aatgtcgaca ccaatcatat atttcatgat aaatccttct ttctttcatt ttaattcaac
    11521 caaaatcctt caatatcttt accaacatcg tcgaaattta aatgaaacgc ttctttcaaa
    11581 atttgactgt cgtattgttc cactgcatca ataaacactt gatgattatg atgtatgcgt
    11641 tcaaaatctt gcgggttctg ttgcatacgt tgtcgcatgg atgttaaaac gagtgccatc
    11701 attactggtt ttaaatgact ccaaaatgag tttaaatatt gatgattaga tgctttgatt
    11761 aatgtttcat gaaattcaaa gtcatgcttc gtaaatgatt ctgcatcctc aaattttact
    11821 gccactttca tcatttcaag ttgtttcttc atttctttta cgatgggtag tcgctcttga
    11881 tttttaactc ttgaaaatgc aaatgactct aacatcagtc gcaaatcata catttctttc
    11941 ttttcttgtt ccccaaacgg caacacatgt gcacccattc tttctaattg gatgagttga
    12001 ttttgttgca ataatttaaa tgcatctcga attggcgaac gactcacatt aaattgcttt
    12061 gccatttgat tttcagtgag taacgtacct tcagctatgt gaccattcac aatgcctaag
    12121 cgtaattctg ccgcgatacc ttctccagtt gtcatacctt ccaaccattt ctctggatat
    12181 ccatacatca tcaaagtcac tccttcatta cacgacatac ttgtatacaa gtatgttaat
    12241 atagttatta tgagtttgca agcgctttct ttacgagcac taaaatagtg accacccctt
    12301 ttcgatttaa atttaaagga aatggtcact atcacacgaa tgatttaatt gttatgttgt
    12361 atgtgggata tttctaattg ttctgtactc atatgcgctt taggtacttc aatgcaataa
    12421 tgcgtttcat gacagtttgg acattcgaat cgacgtgttg tcgctgtatg tttcgctttg
    12481 ataactgccc acaaagatgg tgagaatata tgctggcagt taggacataa ataggcaacc
    12541 ttttgttggt aataaaaagt aacaccaatg ccataaccaa tcataaatgg taaagcaatt
    12601 aaaaacggcc atttattttt catcaaaatt gcacttataa tgctagaata ttgaattatt
    12661 cctataatac cagcactaat ccaaatgtta cgacgaatac ttttcatttc agctgattta
    12721 ctcatgacat gctctatgtc ttttaagtgt gtgattggag acgtcgacgc ttcatttacg
    12781 taatattgaa catttttaat tttgtttaat accgcttgtt gctgtttaac ttgttggtta
    12841 atttcttgtt gtttcatagt tagtaaagta ttgagcgtct tcaaagtacc ttcacctttt
    12901 agcaacatat ctatatcgct taacgcacaa cctaaatctt taagcaataa gattaactct
    12961 aatgtttgtc gctgttgttc tgtatacaca cgacgctttc cttctgtaaa tccttgtggt
    13021 ttcaaaatac ctttgcgatc ataatattga atcgttcgtg ttgtcacatt gcataatttt
    13081 gcgagttctc cagtcgaata gttagacata gattccacct cctataatta ccatagttga
    13141 tgacccgacg tcacgagcaa gtacaatttc cacattttaa agaaatttat tatactaggc
    13201 gtcttatttt tatgatttcg taccatgttg atttacaaac tcactcaaac taagtaacac
    13261 acctactaaa catctactct gttatttcag aatgaatttg ttgtaattta tcttcaactt
    13321 cagtaatctc tgtcgcacat tctttcagta aatctcgata cttttccgtc tctgcattgt
    13381 ttttataacg tattttatgt tctaaacttg cccacatatc catacctatc gttctaattt
    13441 gaatttcaac aggcaatacc tcaacgcgtt ctgctaaaaa gactggaata gatacaacga
    13501 tatgtaaact gcggtaacca ttttctttag ggtgctgaat ataatcttta cgttttatca
    13561 attgtacgtc ttcttgttta agcaacatct cttcaatcaa ataaatatca tctaagtaat
    13621 tacatactac gcgaattccg gcaatatcca gtatatgttc tttggcagtt tctgcgctaa
    13681 tctgtaatcc tttacgatta agcttttcta ttaaactacg catttctttc acacgtcgct
    13741 ccatatgatg tataggattg tgtttataaa tgtgattgaa attgtcatct aaaatactca
    13801 gtttagtgct tatttccttt agtgctgacg aataaatatg atctaactct acgaaaccta
    13861 ataacgtatc aaatgcttca tcaccatttt caaatttact taagctattt ttaaaatcat
    13921 gtcgcaaatc ctctaaatat agtgatggtt ttcgatctac atacattttt atactaacct
    13981 ccgatatata atcatcttta ttgtacctaa cattttatta agataatact aatacactat
    14041 cgaactttgg gctgatactg aaacagcaca aagaaactca aacaattcga attatgtatc
    14101 aaaaccttca attacaggaa gtgattttaa aatgatttaa cgcaaaaaaa cacctgttac
    14161 cgttatatag gtacaagtgc ttaattgata gagatgttat acgtctttaa atgattccac
    14221 aacttttgga tgtggaccat ccataagcgg ttctctttgt cgaacgccac cactttggtt
    14281 gccaattgat tcgctatcaa agtacgattt atcttgattt gattgttctt gaatatgttc
    14341 ttcattatca gttgttgcat attcactatc cacctctgtt ttttccattg ttgctgtatg
    14401 gaattgtacg aagttctctt cttctaatgc tttgatttct tctttcgata acgctcgata
    14461 ccaatctttc gcttttttcg ccgcaatagg aacaacatct ttttcaagat atccttcttt
    14521 taacattgca ttcgcaattt ctatcgcttt ttgccgttta attacttcaa attctttcca
    14581 tttttcgggg taacctttca ttgtaaaggg catttcctta acctccaata tgttataatt
    14641 catgttatat accaccataa ttataaaatg aaacatatta aacacatttt tttagactcc
    14701 cgtcataata acttcagaca aacgttagtc tgacctgata aatatttaat tttaaacgtt
    14761 cttgtctctc tcacgaaaac cattgtgttg aatattcatc attgctgatt gaatataatt
    14821 atactgtggt aaatctggta atatctttat ttctaattct gtatttaatt cgaaatgctt
    14881 agcgatgttt tcaaatattg ctaaatactc gcccattaat tgttcattta ttgtaagtct
    14941 atcttcatta gccatggctc tatttaacat aaagctaatt tcttctaatg cgaataagct
    15001 aggataataa ttttgaatca atgtcttatc actaaataat tcaccattag ctgcattata
    15061 aacttgcgtc atgttattta atttcacaga taggttcata gccgtgtttt tctgatacac
    15121 atcatcttta tattgatttt cagaaaataa ataatgaaag agcgttgctt ctttacgaac
    15181 aacttcagcc attacattag gaagcaatgc ggatgcggta cgttgtccaa ggacaaataa
    15241 acctatgatt gcaatcacaa tacccattgc aacgtcaata actcttggaa acgcaatgtt
    15301 aattgttaaa ttctgtgatg ctaatccgtt cattaaaata acttgtattg taataaaaac
    15361 tactgcaatc gcataatttg ctcccaccaa tgcttcagta aacaatgctg caatgcccat
    15421 taaaattact gcaacaggtg ttggtattga aaacaacaat atgactgaaa gtactaacac
    15481 acctaaaata gtacctaaac ctcgtgccat acctctctcg attgcatgta tagttgatgt
    15541 tcctagtaat attgtatgtg cagataacgg tatccagtat gctttttcaa aatcaaacat
    15601 taacgcaata aatatcgcta tcatcataat cgctgtatat ctcaatgtat ttctaaaaac
    15661 aatagagtct aatgttaaat tttgataaat gcgtttacta tataaaggtt ttcgcatgtc
    15721 tgcttcatac gcaatacgcg cttcatttgc atgcaccatt tcatcaattt tcaatatatg
    15781 gttgaataaa ttttgaaatt cctctgttac tgtcacttct tttcgccaat atcttacatt
    15841 ttcctcacta ttatctagtt gtgcaatgat atgatccatc atttcaatta actcatctgg
    15901 caatggtcga atttgtttag cgttcaactc taacagttca gaataaatcc cttgggcaga
    15961 tgtgtgtaat aataataatt tttggaaacg tctacttaat ttatcgttac ttgaagtaga
    16021 agtgattaat aatttatcag atgctttaaa actatccacc gctgtttttg ctacttgtaa
    16081 aaaagcagat ttatcattat aagtgtgtaa caacttagat atgagtttaa aatcattttg
    16141 aattgcttgt tcttcagctt tgtttttaga aaatacgatc gtgattaaca ccatcatagt
    16201 ggcaagtata ccgcctacta aaatcgcaaa gcctctatat aatgcttctt cgggagctat
    16261 aggtaagtta atgggtagac tgaatgtcac aatgaagaat gtcgatgacg gtccagctat
    16321 ttttaaggca gtaaatatat aaaatggtat aaccgtaaca attaataatg tcattccaaa
    16381 aacgagtggc gttttggctg ttagcgtacc aagcatcata catattgcaa acgctaaatt
    16441 acaaattatt acagttcgca gcttagatcg cgacggacct ttaaaaacat aaatatgggc
    16501 tagtgttccg gttgcaacta ataatccaaa ttggaaatta ccacataagt aaccgattat
    16561 tgcaggaata atcattaaca gtccctgtct tacaccttta taaaagtcta agttaagtcg
    16621 attgattttt atgagtgatg tcaaaaagat gtcatttcga cttctccttt ccttgtcaaa
    16681 atatttccat aatcttttta ttagcatagt taaaatgaat ttgttgctat aaattttaat
    16741 tacgcaacat atgtcattat aacaccttat gctcaattat ctaaatttaa aaacttttaa
    16801 agtccaatta aaattaattt ctaatgttat ttaacatcgt tgaacctttg gtcgcccacc
    16861 tttgcgctag ttaaaattta tatgtacttt tacaagcgtt acctgactaa tgttttaaaa
    16921 ccttatttct ttacatatat catttcaata acttcatttt catcttaaac catatctgtt
    16981 ttaaaaagtt tcaatttcaa cgcaaaaaaa gcaataagcc ccaactatgt gaaagcttac
    17041 tgcttgttta aattattttt ctattgcttt atcatcacta acaattaaag gttgatctgg
    17101 tttcacatag aaccacataa tcacagcgat tactgctgga acgattaaca attggaatgt
    17161 cattgtccaa cccaaatgtt ctacgaatag acctgctaat agcggactga ttaatgcacc
    17221 tatattaccc cataagttca tccaccctga tactgtacca gagaagttac gtcctaagtc
    17281 agttgctgca gcccaactca tacccattga aataccgata ccaccaagac ctagtgataa
    17341 ccagaaaata cttacatata aatttcctgt gcgtacagca aagataattg aaactgcaaa
    17401 cacaataaat cctgcaattg caattacacc tctagctacg aattttgaac gacctaatcc
    17461 taatacacgg tcagaaattg cacctgctga taagattaag aagaacatta ataaccaagg
    17521 taatgaacta atgctcattt ctttaaagtt aacgtggaat acttctgtta aatacgtcgg
    17581 taaccaaatt aagaataatg taatgataaa ttgtacaaca aagtattgaa ttgcaattgc
    17641 atagaagcta aaacgtttaa agaaatcatt ccatggtggt gatgactttt cagtagctac
    17701 gatatcacga ttttccataa tgaaacgttt ctccgcttca ttaaccattc tatgttgctc
    17761 aggtaagtct ttggcaataa tcgcccataa tacagccatt aaaataccta ctgcaccaaa
    17821 aatgtaaaat actgcttgcc agttaaatgc gttaacaata gcaattgtaa ctattggtgc
    17881 taatacaggt ccgaaatatg atcctgctaa taatgcactt gatgctctac ctttttcatt
    17941 tttagagaac caaaatgaat taaacacagc attagaaggg tacattggcg cctcacctac
    18001 accaaataag aatctcacta aataaattaa accgtggttc ttaatcatac ccgttaagat
    18061 tgtaaatgca ctccaccaaa ctaatgcaat tgtaatcatc ttacgtggac cgaatttttc
    18121 agccaacatc cccgatggta cttgcattaa tgcataacct agagagaaga atgatgccaa
    18181 taaaccaaat tgtggttttg tcatccctaa atcatccatc atttgcttag cgatatatga
    18241 aatgtttgct ctatccatat acgcaataac cccaataaag aagaacgcaa gcgcgaacat
    18301 ccaccttacg ttagttcgtt tttccttcat aactattcct acttccctaa ttaatttttt
    18361 aaaacttacc ctgaaacgtt aaaattttcg gacatctggc atgtccaccc ctactttgtc
    18421 acaaaattgt accaaagtta ttttttataa tattatcaga catggaaagc gtttacaata
    18481 gaatctataa aaattttatt aaattccgct taacttttgc tattaaaacg atataaaggt
    18541 acttttagtt acatagtagc ctatcgtttg ttaattaaaa atactgcaat cctagtgttt
    18601 taaaacgtat attgcaggct ttcttacata ttcgacattc attttaaatt gcatataact
    18661 acattaacca ttcattgtgc agcgcctgtt gtttagatga tcatttattg actttttgct
    18721 cttatagcaa gcaatttttt gatttattgc ttataataca ttttaagaca aatgtttgtt
    18781 tcaacatcgc atgcgacatc attaattatt catttgaatt tatccagttt ctatttgtct
    18841 gcaattgctg aaaattaaag gagcttattt ttaataatgg aacaaattat cactgaattt
    18901 attagccgtt ttgggtatgc agccattttt atattaattt tattagaaaa tgtactacct
    18961 atcgtaccat cggaaattat tctgacattt gctggtctta tgtctgttaa atcacattta
    19021 tcaattttaa ctttatttat tattgcaact atcgcatcgt ttatagggct gttaatttta
    19081 tattatatct gccgtttgat ttcagaagaa cgtctatatc gttttattga tcgacacggt
    19141 aagtggatta aattgaaaag taaggattta aagcgagcaa atgattggtt taaaaagtat
    19201 ggcgtatggg ctgtatttat ctgtcgtttc atacctgtat tacgtgtatt gattaccatt
    19261 ccagctggtg taaaccgcat gaatgttgtg acatttaccg ttatttcatt aataggtact
    19321 acaatctgga atttcggttt aattttacta ggacgcactt tgagcgatag ctttggtatg
    19381 ttgatgactg gtcttcatac atattcacgt attatgtatg ttgtcatcat tatcgcagtc
    19441 atttactttg ctatacgtta cattggcaaa cgtaagagag taaaataata ttgttgttag
    19501 cataaaggca tgaattttaa atttctataa acaaaaagca gtgaatcatt ccatctattg
    19561 aaatgattca ctgctttttt aagtttattt ggcatacttt agctgaaagt caactatctt
    19621 tatataaaga aatattacta ttaacatgat agttatagtt gtgaagtaaa aggctaccaa
    19681 cggctgagaa atgataccaa ttaataatgc accgacagtc attccaatat atctcacaaa
    19741 attaaataca cctagcactt tcccttttat atgttcaaac cttttagaaa ttatagtaga
    19801 cagcaaagct ggcacaatac caacacaagt acctaatata aatataatta ctgataatat
    19861 aattacattt gattcgttta accatccaaa caataacgta aatatagcaa atccagttat
    19921 agtaacacga agtatcatta tttcattata tttttttgaa aatcgcttat aaaataaact
    19981 tcctaatata acactaacag taataggtaa aaacaaaact cctgatattc caacgcctat
    20041 atcataggta ttatttaata atgttggtaa ataaactaat attgaaaagt acgtaaaaaa
    20101 tattaaaaat cccaatgtca ctaaagtaat aaacacttta tcggtcaaat atttttcttc
    20161 aattttttca gttaatttta caatcccttt ttcaacattt ggaatatcaa taaaaaagat
    20221 aatcaaaaat aatacgatag atataacgag caaaaaacta aaaataccaa tataatccca
    20281 tcttgcacct aaagtactcc ctaagattgg tgccaaagct ggtgcacatg ataaaaatat
    20341 ttgatagttc gccatagctt gagctctacc attatctgta cttagtttgg ctaataaagt
    20401 taaagtcacg agaggaataa ttccgcaacc aattgcttga aataaccttg atatagcaaa
    20461 taatataaag ttatgtgtca ctgcacaaat aaagctagat atgattacta ttcccaatcc
    20521 cgttaaaagt acttttttcg aatcacgaga atctatactt ctaccaagta caatttgcat
    20581 aactgcaacg ataaatataa aaccgccaac tgtcgcattt atccaaaata gtggtacttg
    20641 aaaatcgtca tatagtctcg gaataatagg cgtgtatatg ttttgaatca atgatgcgaa
    20701 aaatgctaaa aatcctagaa tatataatct gatattacta ttaatcattt aaaccatctt
    20761 ctttttttaa ttcttctata ctacggttta aaaactctat aataactgcc atttcatcat
    20821 catcaaagac tttaagtacc tcttcatatt tttttaccga tttttcatgt aattcatcat
    20881 gtatataaga taattttttt cctttttgtg ttaatgaata attaacttct cttttgttaa
    20941 attcattgtg cgattcaact actaagtctt tcgctaaaag tttttttatc gatttagtaa
    21001 ttgctggttt tgatacattt aacatttcag acaaaaattt attattcact ttatcattat
    21061 tatcaatcaa ctctatgata tgaaattgtg ttagagataa atctagttcc tcattttcct
    21121 ttttatactg tcttctttta tcggcatttt ctctttctat aataaattgt tgtaatttta
    21181 aaataacttc tctcttcata tccactatta ttcctcctct tcattagtta accggttaac
    21241 tatattattg ttgtcactcc catttttgtc aaatattgtt gtaattaaca tatatttact
    21301 tagttatgca ccatttattc cgcatttcac aaatccacct ataatacaaa ctatcatttt
    21361 aaataagcag aatttaccct gtcttccaca caaattgttt tcgacacttt aatgtgccta
    21421 ctatccaaaa aagttactca taacaaattg cgcattttaa taaatgtagc aatgccattt
    21481 gcatgctgca aaaatactcg tattcatatt tatgttttat attattaaag ttttttaaat
    21541 ccatattttt ataacacttg ctatgtgata aaattaattt tatatataaa atcttaagat
    21601 tcagattatt taatagcaaa ggagatagtg atatggatgt tttaacaata gaacatttaa
    21661 caaagaagat aggcaacaaa acgattctcg aagatgtatc atttaagctg aaacgcggac
    21721 aaatagttgg tctcgttgga gcgaatggtg caggtaaaac aactttaatg aaagttatat
    21781 taggttactc tagtttccaa agcgggaatt ttaatgttat taacagcaag gacagcaaaa
    21841 gcaatatcgg cgcattgatt gaaaatccag gaatatatcc ttttatgtct ggatatgaaa
    21901 acttgaagtt attgaatgaa tcaaaaaaca ctcaagatat cgataaaatt gtctcacaac
    21961 ttcatatgga tgaatacatt cataaaaaag ctaaaacgta ttctcttggt atgaaacaaa
    22021 aattaggaat tgctatagca tttttaaata aacctcaatt cattatctta gatgaaccaa
    22081 tgaatggctt agatccaaaa gctgtgcgag atgtacgtga attgattgtc caaaaagcgc
    22141 aagaaggtgt tactttctta atttcgagtc atattttaag tgaattagtt aaaatcacaa
    22201 actctatcct tattattaac aaaggtaaaa ttgttacaga aacatcggaa gaagaactta
    22261 aacaatttaa agataatgat ttagaaaatg tattactaga aatcatagaa agggaggacc
    22321 aagcataaaa tgggaacttt aattaaacaa gaatgtttca aattatttaa aaagaaatca
    22381 acttttatcg cacctattgt ctttattcta ctaatggttg ctcaaggtta tattgctaca
    22441 aaatacaatg aaatttttac gccacaggaa tctttcacat ctgcttataa tggtttttca
    22501 tggtttgcat ttttattaat tattcaagca agtacaatca tttcaatgga atttcattac
    22561 ggtacgatta aaaatttact ctatcgtgaa tattcaagaa caactatgat tgttagcaaa
    22621 atcatcacat tatttattat ttctttaatt tattttgtta ttacaattat tgcttcaatt
    22681 gttattgggt ctttattctt taatgattta aatatatttg aaagtagcgg taatcaatta
    22741 tctttattga atcaattatt attagttagt ttaggcacat ttgttggcgt ttggttagtt
    22801 ttaagcttaa cgttgctatt atcatctgca acaaattcaa cgggagtagc cattgctgta
    22861 ggtattgttt tttattttgc aagttctatt ttagcagtta ttcaaacggc acttttagaa
    22921 aaaatagact ggctaaagtg gaatcctatt aatatgatga atattatgct tcaaacagtt
    22981 gaaaaaggct ttagtaagtc gacaaaatta gaacttcatg aattgtttat tggtaatatt
    23041 gcttatattt ctattttctt aatacttgta gtatttattt tcaagaagaa aaatatttag
    23101 taacttaaag tattaaatgt ctaaatacac acatattcca tcgtaattca aaatcatttt
    23161 caaatccctt cacccaaata atggtgcggg gattttttca tccaaatttt ggaaattcca
    23221 cattttacat atcgtaattt tttaggaaac tagtgaatat aacaaatccc tcctctcatt
    23281 tttaaaatag atatatcact tccccacttt tacttaacta aactgcaacg gttcctaata
    23341 ccaaaatcct gccctctatt tttatcaatt caagcatact tattgaaaaa tgttaacgtt
    23401 ttcttgataa tcattgtaag cgcatttatt ttataaacta acgtttgaaa tatactacag
    23461 gagtgacacg taatgactca aattactgaa aaagaattaa aaaagaagta tttagattta
    23521 ctatcccaaa attttgatac tccagaaaaa cttgcaactg aaattatcaa tttagaatca
    23581 attttagaat tacctaaagg tacggaacat ttcgtcagcg atttacatgg tgaatatgaa
    23641 gctttccaac acgtattacg caacggttct gggaacgtgc gagcgaaaat caatgatatt
    23701 ttcaaagaga gactttcaac taaggagctt aatgacttaa ctgctcttgt ctactatcca
    23761 gaagacaaat taaaattgat taaaagtgat ttccaaaatt gcggtcaact taatgtctgg
    23821 tatatcacaa caatcgaaca tttaattgag ttaattaaat attgttcttc aaaatatacg
    23881 cgttcaaaac tgcgtaaagc attgccaaaa caatacgttt atattattga ggaactactg
    23941 tataaaagta atgaatatca aaataaaaaa tcatattacg aaacacttgt taaccaagta
    24001 attgaactta aacaggcaga tgatttaatt attggactcg cttattccgt acaacgctta
    24061 gtcgtcgatc atttacacgt tgtcggtgat atttatgatc gtggaccaca accagataaa
    24121 attatggata cactgattaa ttatcattcc ctagatattc aatggggtaa tcatgatgtg
    24181 ctttgggttg gagcctatgc tgggtcaaaa gtatgcttag caaacttact tcgaatttgt
    24241 gcacgttatg ataatttaga tattatcgaa gacgcttatg gcattaattt aagaccactg
    24301 cttactttag ctgaaaaata ctatgacgca gataatcctg cttttaagcc taaaaaaaga
    24361 cctgacaaac acgaacgttt aactcaacgt gaagaaagtc aaattactaa aattcatcaa
    24421 gctattgcga tgattcaatt caagttagaa ataccaatta ttaaacgtcg tccaaatttc
    24481 gaaatggaag aacgtcttgt gcttgaaaag gttaattatg atacaaatga aattacagtt
    24541 tatggtaata catacccatt gaaagacaca tgtttccaaa ctgtcaatcg tgataatcca
    24601 gcagaattac tacctgaaga agaagaagtc atgaataaac tattattgtc attccaacaa
    24661 tctgaaaaat tacgtcgtca tatgtctttc ttgatgcgta aaggctctct ttacttacca
    24721 tataatggca atttactcat tcatggttgt attccagttg atgaaaatgg tgagatggaa
    24781 tcatttgaaa ttgatggtca tacttacagc ggccaagaat tattagatgt gtttgagtat
    24841 catgtccgta aatcatttga tgaaaaagaa aatactgatg acttatcgac ggatttagtt
    24901 tggtatttat ggactgggaa atattcgtca ctatttggta aacgtgccat gactacgttt
    24961 gagcgatact ttattgcaga taaagcttct cataaagaag aaaagaatcc gtactatcat
    25021 cttcgtgaag atgtgaatat ggttcgtaaa atgctcagtg atttcggatt aaatccagat
    25081 gaaggacgca ttattaatgg tcacacacca gtgaaagaaa tcaatggcga agatcctatc
    25141 aaggctgatg gaaagatgct tgtcattgat ggtggctttt caaaagctta tcagtcaaca
    25201 actggcattg caggctatac actattgtat aattcattcg gtatgcaact cgttgcacac
    25261 caacaattta acgctaaaga aaaaatactt tccgaaggta tcgatgaact ttctataaag
    25321 cgcgtcgtag ataaagaatt acaacgtaag aagattcgtg ataccaatat tggtaaagat
    25381 cttcaagctc aaatagatat tttgaaaatg ttaatgcatg atcgttactt agactaaaga
    25441 cgacatttga tgcatgtatc gtgcacttat ttagtggtac tgtgtttacg cattggctac
    25501 gcaaacacga tgataaatag gcgacctagc tacaccgaaa aaaatcccct cactattgcg
    25561 agtagtgagg ggattggtgt atctatgcta attcctgtta actgtattat aacaacttta
    25621 cagcaaaaaa atgcaaaatc aactaataaa ttaatgaaat gaccgacacc ctcgcttcta
    25681 ctccaattta acaaaatact atattagtac tatatttttc attaacatgt atgttacatt
    25741 ttctatatta cttaaaatta cgaagggaca tttttaaatc atgcgtatct taaaagagtc
    25801 cattattgtg gcatttgcct ttgttggtgt tgtcgttggt gccggctttg ctactggtca
    25861 agaaattttc cagtttttca caagtcatgg cgcatatagc atttcaggca ttattgtaac
    25921 aggactattg attactttag gtggaatggt tgtcatgcat acaggtcatc atctaaagtc
    25981 cagaaatcat tctgattcaa ttaactattt cttatacccc tctattgcaa gaggttttga
    26041 tattatttta acaatgttta tgttgtcttt agctattatt atgactgcag gtggtgcgtc
    26101 aaccattcat caaagtttca acttaccgta ttggctgagc gcactcatat tagtcgcctt
    26161 tattttagca acactgtttc taaaattcga tcgtttaatt gctgtgcttg gcggtgttac
    26221 cccattttta attgcgattg tcattatgat tgcggtctac tatttcacaa caagtcatct
    26281 tgattttact gccgctaata atgatgctca gattcataag cagaaatcat tatcacctgg
    26341 atggtggttt gatgcgatta actatgcaag cttgcaaatt gctgctgcct tcagcttctt
    26401 atcagtgatg ggtagtaaag ttaaatatcg tgactcaacg ttatacgggg gcttgattgg
    26461 cggtttaatc attacatttt tactcatgat gattaatcta ggtttaattt ctcaattcga
    26521 taaaattaaa cacgtagatc tacctacatt aaaattagcg acacaaatgt ctccgtcaat
    26581 tggtattatt atgtctgtca ttatgatact tgtcatctac aatactgttg ttggattaat
    26641 gtatgcattt gcgtcacgtt tcagcgttcc attcagcaga cgttacttca tcattattat
    26701 tacaatggct gtcatcactt atattagtac atttatcggt ttcatttcat taattggaaa
    26761 agtattccct attatgggat tgttcggttt catcttactc atacctgtac tctataaagg
    26821 tttaattaag cgtattaccg gcaaatctca tatcgattaa aatatctata ctattgtaaa
    26881 atttagtaca gcacttcaaa atcaaaaaag cgggcagatg aaaagtaaaa atcttttcca
    26941 tctgtccgct tcttaataga actgttattt cccaagtatt tgttgaccag ccgttaatcc
    27001 agtttctgta atttcatggc ccttcaccca aacttcttcg acttgtgccc cacgtgtatt
    27061 aaacaagtta atgacttgtt cacttgcagc taatggcaca attggatcat gtttccccat
    27121 agaaagcaac acactgacat ctgataaatc ctttgttgac gttacttcaa ctgggtataa
    27181 cggtgcatat aacaatgctt ttttaaatgg tgcttctgaa cgcaacatta agttaatcgc
    27241 tatatttgat ccatttgaaa atccaacaag tactgctttt tcaatatcaa aatcataacg
    27301 ttcagcagct tctttaatga acgtcaacaa ttcttgtcca cgaaatgcca aatcttcttc
    27361 atcataaaca ccttcaccaa gacgtttgaa ataacggttc atcccatttt ctgaaacttg
    27421 tcctctaata cttaacaagt gataattttc attcaatgct tcgcctaacg gtaataaatc
    27481 gaactcatca ccacctgtac catgcaataa tattagtgtt ggcgcaccat tttgtccttc
    27541 tctaaaaata tgttccatca caaatcctcc tcattccaat taaccatgtt gacgcttcgt
    27601 attaaaaggt ctaacttccg attcaatata ttctctttta ttttctaaaa atggtggtaa
    27661 ggataaccct tcgcctaatg tttcataagg ttcatcttcc ataaatcctg gtccatctgt
    27721 tgaaatttct attaaaatat gccccacacg tgcatataat gcttcaaaat agaaacgatt
    27781 aacgatgcct gagttattaa tacctacctc tttatatttc gttgcccacg cttctattgc
    27841 atcatgatct ttcacacgaa atgacacatg atgtacctca ccataacctt gacgtgctgc
    27901 tggcccttta tcatctttta ttaagattac ctggccacca ttgcctcctt cgccaacttc
    27961 aagtaatgcg acattatctt catgcgcaat agttgtcata ccgtaaacag tctctaaaat
    28021 atttttaaag tcgtcaaaat aacttacttt aatttcaatg gggcctaatc cataaatcgc
    28081 tttatctact ggaaccggtc cattcttcca aggtacacca ggtgctaccc cttcatttaa
    28141 ctcatctgaa attaattgat acacttggcc atcgacttct tcaaatggca acactttttt
    28201 accaaataat tcttgaatac cttcgtgttt aacaccaaac tcatcaaagc gctgttcata
    28261 atatgttaat gcgtcatcgt taggcactct aaaagacggt cttgtaatgg aatttgttcc
    28321 tgcctgccct tttgtaatat ttggaaaatc aaagaacgtc atgtctgtac ctgccgaacc
    28381 tacatcatct gcaaaaaaag tatgatacgt ataaatatca tcttgattga ctgtcttttt
    28441 aactaaacgc atgcctagta cttctgtaaa aaatttataa ttacgttctg catcatctgt
    28501 cattgcagta acatggtgaa tacctagtaa ttcatgatta tttatcattt tatataccct
    28561 ctttatattt ttagtgcatt tatctcgaaa tcgagatact ttaatgtaaa tttttagtgc
    28621 acgccttaca cttctgtaga ttgtgcactt agtttcttaa acgcttgttg taagattgtt
    28681 aattcatcct ttgttaacac atcaaacgct tttgttaatg tctcagcatg cttagggaaa
    28741 atatctgcca tttgactttg acctttttca gttaaacaag ccatatatac acgtttatca
    28801 tctttatcct tttcacgtgt aatccaacct ttgtcctcta attgacttac aacatatgaa
    28861 atgctgctac ttgcaattaa tacgcggtct ctaatacgtt gaattggttg cggaccttta
    28921 ttataaagca actcgagcac tgcaaattca gtaatattta agccatatcg ctttacgtct
    28981 tcttttgtaa tttgctctaa tgtgtctaac gccctattca ttccgacaaa aacattgaga
    29041 gattgtttcg ttcgatccat atgatcacca acttttttat tttatttcga tttcgagata
    29101 aattatatag ctgaatagat ttaatgtcaa gatacgtgtt ttcatcttca tttcaaactg
    29161 tgcttaataa aaacgactac atagatgtgg caatgtcgtc acatgactgt catgcacctt
    29221 tgacttcgtt taagttaaaa tagaataggg aaatactata atcaatcatg ataggagttg
    29281 atatgtatga gtaaccttga aatcaaacaa ggcgagaaca aattctatat tggtgatgat
    29341 gaaaataatg ctttagctga aatcacatac cgttttgtgg ataataatga aattaacatt
    29401 gatcatacag gcgtatctga tgaacttggt ggtcaaggtg ttggcaaaaa actacttaaa
    29461 gcagttgttg aacacgctcg agaaaataat ttgaaaatta ttgcctcatg ttcatttgcc
    29521 aaacatatgt tagaaaaaga agattcatat caagatgtat atcttggtta aataaatttt
    29581 gcgatggtgc aactgtacca tcgctttttt gttcgcatca atataaaatt gcaaaacgac
    29641 tagttatttt gcatttcaaa ctttatgcta tcaattcatt ttcttttctg gtatactttt
    29701 tatactaact tatagaaaaa tagaaaaaga ggcgatgcat aatggcattt cacgataaaa
    29761 cagcaacaca agtgacaaac attgtactga acgtaagaga cttagactta atgacaacat
    29821 tttacaaaaa cattttagga ctatccgtta aaagttctga cgataataca accgtattat
    29881 ctgttgggac tggtggccat actctaacgt tacatttatt agaagacggc cgtcagactt
    29941 ccccacgtga agcagggctt tttcatatag catttttatt accaactact gaggatctag
    30001 ctaacttctt atatttcgtg gcacaaaaaa atatgggcat cggcgctggt gatcatttag
    30061 taagtgaagc tttatatttc aacgaccccg aaggtaatgg tattgaagtc tatcgcgata
    30121 gaccttcatc ttcatgggaa tggcaaaatg gcaaagttaa aatggatact ttagaagttg
    30181 atagccaaac cttattaaca catcgtactg atgaaggttg gcaaggaatg ccagcaaaag
    30241 gcatgattgg acacttacat ttaaaaacac atgatttaga cgcagcatat caatgttaca
    30301 ttgaacaatt aggattccaa catgtgtctg attttccacg tgcgctattt atgtcaacga
    30361 atcattatca ccatcatatt gctgctaata cttggcaatc caatcaagca agacaaaaca
    30421 attcacaaag ttatggatta acacatgttg atatttatca acccgacgcc tcaacacata
    30481 catttacggc accagaaggg tttgacatca cagttcattc aaatactgac cttgttccag
    30541 aaaaataatc atccatactt tattaataca attttttcac gcaaaagcct gcgattcatt
    30601 tatgtaatcg caggcttttt tattgctaac tttttaattt tatatattta gacatacggt
    30661 attctattta ttcttttggt ccaacccatt caataacatc ttcataagct tggcgtgttt
    30721 tatttttcgg tggctcttgt tgtctgtagc caaatgcgac catcactgat aaaccaaatt
    30781 gctcagtatc taagatccct ttatttgcta aaatgtctgt cactgtatcc agactaaaac
    30841 cttccatcgg acatgaatca atacctaaca atgcggctgt cgtcatcata ttgcctaatg
    30901 caatatacgt ttgtttactt gaccagtcat acaaggcttg atcattatca gaaatatgga
    30961 aatctgcttg gaatgcatcg aatttttgtt caacagctgg aatcgtttgt gcctcatatt
    31021 ttttaatatc tcttaacata tgttgtacat acggtgatct tgacgttaca tttttacgcg
    31081 caaaaattag cacaaaatgg ctcgctgtat ctaattgttt tgctgcgccc caactgtgcg
    31141 cttttaattc atcacgtaac gcttgatctt gaatcacgac aaacttccaa ggttctaacc
    31201 caagagaact tggagacaat ctacctgact ctaatattgt ttcaaaatct tctttcgaaa
    31261 ctttcttttg tggatcgaat tgcttagtcg catgtctgaa atgaaatgca tccataattg
    31321 tttgattcat attgctcatt gaacatagcc tcccattttt atatttattg ctatacaaat
    31381 tatacttcat gttttaaaaa gactaaagta atccgtttgc aattcactat aacttgtgca
    31441 catgttataa gttttctgca ataaaacaca cttttgaatt ttgggaaatt tagtgttata
    31501 atatatttgt gatatttttc acaattaaat tgtggcgtga ttttaatgtg cataatcttt
    31561 aatttcactc atttactttt caatctttta aaagcgaggt ttttaataat gacaaaaatt
    31621 atgttctttg gtacgcgtga ttatgagaaa gagatggcat taaattgggg gaaaaagaat
    31681 aatgtcgaag taactacttc taaagagcta ttatcaagtg ctacagtcga tcaattaaaa
    31741 gattacgatg gcgtaactac aatgcaattt ggtaagttag aaaatgacgt ttatcctaaa
    31801 ttagaatctt acggtattaa acaaattgca caacgtactg ctggatttga tatgtatgat
    31861 ttagatttag ctaaaaaaca caatattgtg atatctaacg ttcctagtta ttcacctgaa
    31921 acaattgcag agtattctgt ttctatcgcc ctacaattag tgcgtcgctt cccagatatt
    31981 gaacgccgtg tacaagcaca tgattttact tggcaagcag aaatcatgtc taaaccagtt
    32041 aaaaatatga ctgttgcaat tattggtacg ggtcgtatcg gtgctgctac agctaaaata
    32101 tatgcaggat ttggtgctac aattacagct tatgacgcct atcctaataa agatttagac
    32161 tttttaactt ataaagatag tgttaaagaa gctattaaag atgccgatat tatttcttta
    32221 catgttccag cgaacaaaga aagctatcat ttattcgata aagcaatgtt tgatcatgtg
    32281 aaaaaaggtg caatcttagt taacgcagca cgtggtgcag tcatcaatac acctgattta
    32341 atcgctgcag tgaacgatgg tactttgtta ggtgctgcga ttgatactta tgaaaatgaa
    32401 gcagcatact tcacaaatga ctggactaat aaagacattg acgataaaac attattagag
    32461 ttaatcgaac atgaaagaat tttagtaaca ccacatattg ctttcttctc tgatgaagca
    32521 gtacaaaacc ttgttgaagg tggtttaaac gcagcattat ctgtcattaa cactggtaca
    32581 tgtgaaacac gtttaaatta atatatatat ttaaaagcaa aggcgcgcca tgtgcctttg
    32641 ctttttattt tgtatatctg tatcaaaatc gatttgatta aaatccgctt tattttatca
    32701 tctattcaaa tgattttagt gcgcttattt tactatggca ttattgccta acctgtttga
    32761 gatatattaa tatttgatga ttcgtattca aagttttcat ttaacatgta tttagtatca
    32821 tgatagctgc ttcattgatg atatactacc tactttttca acacttcttg ttcgataatc
    32881 gttaatacac cctgcttatc attacttgga gcgacagcag aggcaatatt gaacaattct
    32941 tcatcatgac tattttccat cacataacta tgcttagcga acgctaacat atctttatca
    33001 ttattcgcat ctccgaaggc catgagctct gaaggagaca tttcccattt atctaacaat
    33061 cgttttaatg cctgcccttt agtcatgttt ggcataataa tatcaatgct atcgttccca
    33121 cttgagacaa gtttaatatc attgctgaat tgcgttgcta cttcttcgtc aacattcgga
    33181 tgcgtctcac gattaatatt aaatgctatt ttgacataat catcctcagg taattgctgt
    33241 agactgtcaa tttcttttaa ctgatgataa taaaatctcg tatcttcttt aaacgcttca
    33301 gaagtatgtt ttaaaatata cgcgcttttc aaaccacaga tgacgagttg atcaatactt
    33361 tgcttcatat ttaagtaatc gacaacctgt tgaaacacct gacgattaaa gcttttataa
    33421 ttatataact cattgccatt ataaataact gcaccatttt cagaaataaa atacatatct
    33481 ctatccccga aaatagactt caacttcgca tattgattgc cactcgcagc aataaatgta
    33541 atatctctat ttctaagttc agtaaaaatc gcttcaaatc taagtttatc atatgtcttt
    33601 tttgaatcta aaaacgtccc gtccatatct accgcaatag ccttaaccat gattatcctc
    33661 ctcattgctc ttttagtcgt cttcattata tccatctcat gacaagcaat gaagtttgtc
    33721 ataccacaat gaacctatat tcacattttg aaattgcttt catatatgca aaacacatta
    33781 cactggatat agcattcaga ggcgaacata aatttcagaa tattgtaaaa atatcacact
    33841 atatttatca tttagacaaa atatagtgtg atatactaat ttataacatc tatacataat
    33901 gtgaaagaat ttttaaaaat acaattaaat atcggtgtaa taaatccctt tatacatagg
    33961 agttgtatac atgaaactaa ataactattc tttaaaagtt aaaaacaaac aactagttga
    34021 caattgtgat ttaaatttct atcttggtca gatcaatcac attgttggta aaaatggtgt
    34081 aggaaaatct ttattagcta aagatttctt actaaataat agtggaaata tccctaagtc
    34141 catttctcaa aatgtaacct taatatcaag ttcatcaaat attcctaatg atataacaaa
    34201 agatttttta ttatcattgt taaaatcaaa atttgaaaac aatcgacaaa cattcgataa
    34261 gatttataac atactaaaca tcgaagcaat accgtctaac gtactactaa aaaacttgag
    34321 tgatggtcaa aaacaaaagc ttaaattatt aagtttctta ttggaagatc atgatttaat
    34381 catattagat gaagttacaa acgctttaga caagaaaaca gttaatgaaa tttatgaatt
    34441 tttaaatgat tttattcaaa gccatcaaac taaaactatt atcaatatta cacataattt
    34501 atccgattta agtgctttgc cgggtaaata ctttattttt aaagaccttc aaatagaaga
    34561 gtaccaatca aaagaagaag tcataaatga ttacattaat ttataattga ggtgtccact
    34621 ttgaaattag aatttaaaaa aagtatttcc aataaaatta tttacacgct tgttgtgcta
    34681 ttcacattcc ttttcttact tgggtatttt ttaccaatag gtatagacaa agttaaaagt
    34741 ttaagctata gccagttttt cttcagttca tatactgttg caacgcaatt agggttctta
    34801 ctattttcat ttgttattgc atattttatt aataaagagt attcgaataa aaatatcctg
    34861 ttttataaat tgattggcga taatatcttt accttcttct ataaaaaggt agcagtgtta
    34921 ttttttgaat gtttagtgtt cataattcta agtatcacta ttatttcaat tatatattcg
    34981 gatttctcac attacttact attaataatc ttattttcac tcgttatctt acaatatatc
    35041 ttagttgttg gaacgattag tatggtttca cctaacattt taatcagctt aggtatcagc
    35101 attgtatatt ggattggttc agtcatctta gttgccatta ataaaaatat atttggtatc
    35161 gttgctccat ttgaggcgag caatacgatg tatcgtgccg tagaaaaaat attgaataac
    35221 gagtcaacat ttatgtgtcc tactgaaatt ataaatatag tatcattctt cgttcttcta
    35281 tttatagtta atactattgt cttattacta agtagaaaga gatggttgaa aatcggtatg
    35341 tagtataaaa aaggagcggt atacattgcc gcttcttttt attatgtatt tttacaatct
    35401 ccaccaatga cacgtcgtca ttgctacctc ataccaaaca aaaaacagtc tcgcaatcaa
    35461 gactgttttc cactcaatat attcatccat tagcgtaata gattatttga cttctgtagc
    35521 tacaaagatt ttacgttttt cccaaacgcc tgtcttttca ttgtaatcat cacaagtaat
    35581 taatgttaat tgtttatctt tacctttttg ttcatctaga actcctacat ctgtaggctt
    35641 aacatctctt atacttgtca ttttatactt acgtgtttca ttaccaactt taaagtacac
    35701 catactacct tttttggctg ctttaagatt tgtaaattga tagttcggac ggtcaatgaa
    35761 agtgtgtcct gcaattgaaa tattttgatc atctagtgat tcattttctt ctgcaaagct
    35821 tacacctcta tttaattgtt caggtgttgc tggtcctgga tatactggtt ctttaatatc
    35881 agcatctgga atttcaatat agcctgccac tttcgattta tctttcggaa tttgaggttt
    35941 agcttgctgc tttttatctt tactcgcctg ttcttttaca tttttatcat attgttcaat
    36001 cttttcatct ttatctttat cgtgaagata attatcgata tgtggtttag caaacaaata
    36061 tgctgccact aggataagta ccacaccagc gattgtcatt aatcgatttg tccatttttt
    36121 catacgttaa ggctcctttt atacatttca caatgcctgt taacatttca tatagtatac
    36181 cattaatttc aaaatgactc atagcaattc attttatact ataaaattta catgtatact
    36241 tttacgttag atttcattac acatatttgc attcaaataa cgaaacgctt ttaataatta
    36301 ctaaggggga attgatatga ttagatacgc taaaaaagag gatttaaacg ctatattagc
    36361 gatatacaat gatgccatta tcaatactac agctgtttat acttatgaac cacaaaccat
    36421 agacgaacgt gtcgcatggt ttgaaacgaa acaacgtaag catgagccta tctttgtatt
    36481 tgaggaaaat ggaagtgtct tagggtttgc gacgttcggt tcatttagac cttggccagc
    36541 atacctatat acaatcgaac attctattta tgtcgatgct tcagctagag gaaaaggtat
    36601 tgctagtcaa ttactacacc atttaattgt ggaagcaaaa gctaaaggtt atcgtgcgct
    36661 agttgcaggc attgatgctt ccaacaaagc gagtattcag ttgcatcaaa agtttgcttt
    36721 taagcatgcc ggcacactga ccaatgtagg ttttaaattt aatagatggt tagatttagc
    36781 attttacgaa ttagatttac aagactagta atgtttgaat cacataatat aaacaagaca
    36841 accatgttaa ttcccttaac ataacaagcc aacatataaa attttaaact tctcagggga
    36901 gtgggacaga aatgataaag agccactaat gatttattat gtagtggttc ttacacatta
    36961 gccacagcta atgtgtactt aaaaatagga atacatgagt aaaact
//
LOCUS       DALAAR010000022        36896 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000022 DALAAR010000000
VERSION     DALAAR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.022"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O2458_001112"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O2458_001112"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ3886985.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O2458_001113"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O2458_001113"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ3886986.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O2458_001114"
     CDS             complement(1484..1927)
                     /locus_tag="O2458_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCZ3886987.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2127..2540)
                     /locus_tag="O2458_001115"
     CDS             complement(2127..2540)
                     /locus_tag="O2458_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCZ3886988.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2998..4371)
                     /locus_tag="O2458_001116"
     CDS             complement(2998..4371)
                     /locus_tag="O2458_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCZ3886989.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(5043..5273)
                     /locus_tag="O2458_001117"
     CDS             complement(5043..5273)
                     /locus_tag="O2458_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886990.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(5304..6242)
                     /locus_tag="O2458_001118"
     CDS             complement(5304..6242)
                     /locus_tag="O2458_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCZ3886991.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(6677..7342)
                     /locus_tag="O2458_001119"
     CDS             complement(6677..7342)
                     /locus_tag="O2458_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ3886992.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            7494..7814
                     /gene="czrA"
                     /locus_tag="O2458_001120"
     CDS             7494..7814
                     /gene="czrA"
                     /locus_tag="O2458_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCZ3886993.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            7816..8796
                     /gene="czrB"
                     /locus_tag="O2458_001121"
     CDS             7816..8796
                     /gene="czrB"
                     /locus_tag="O2458_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCZ3886994.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            9062..10153
                     /locus_tag="O2458_001122"
     CDS             9062..10153
                     /locus_tag="O2458_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3886995.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(10653..10757)
                     /locus_tag="O2458_001123"
     CDS             complement(10653..10757)
                     /locus_tag="O2458_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886996.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(10918..11401)
                     /locus_tag="O2458_001124"
                     /pseudo
     CDS             complement(10918..11401)
                     /locus_tag="O2458_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(11444..12580)
                     /locus_tag="O2458_001125"
     CDS             complement(11444..12580)
                     /locus_tag="O2458_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCZ3886997.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTD
                     IDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            12869..12961
                     /locus_tag="O2458_001126"
     CDS             12869..12961
                     /locus_tag="O2458_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3886998.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            complement(13666..14523)
                     /locus_tag="O2458_001127"
     CDS             complement(13666..14523)
                     /locus_tag="O2458_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ3886999.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(14591..15373)
                     /locus_tag="O2458_001128"
     CDS             complement(14591..15373)
                     /locus_tag="O2458_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887000.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(15605..17410)
                     /gene="glmS"
                     /locus_tag="O2458_001129"
     CDS             complement(15605..17410)
                     /gene="glmS"
                     /locus_tag="O2458_001129"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCZ3887001.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            18091..19629
                     /locus_tag="O2458_001130"
     CDS             18091..19629
                     /locus_tag="O2458_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCZ3887002.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            19664..21796
                     /locus_tag="O2458_001131"
     CDS             19664..21796
                     /locus_tag="O2458_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ3887003.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            21808..22242
                     /locus_tag="O2458_001132"
     CDS             21808..22242
                     /locus_tag="O2458_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ3887004.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            22242..23348
                     /locus_tag="O2458_001133"
     CDS             22242..23348
                     /locus_tag="O2458_001133"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCZ3887005.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(23667..31103)
                     /locus_tag="O2458_001134"
                     /pseudo
     CDS             complement(23667..31103)
                     /locus_tag="O2458_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(31380..32735)
                     /locus_tag="O2458_001135"
     CDS             complement(31380..32735)
                     /locus_tag="O2458_001135"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCZ3887006.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(32762..33694)
                     /locus_tag="O2458_001136"
     CDS             complement(32762..33694)
                     /locus_tag="O2458_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCZ3887007.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(33696..34505)
                     /locus_tag="O2458_001137"
     CDS             complement(33696..34505)
                     /locus_tag="O2458_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCZ3887008.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(34694..35602)
                     /gene="rocF"
                     /locus_tag="O2458_001138"
     CDS             complement(34694..35602)
                     /gene="rocF"
                     /locus_tag="O2458_001138"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCZ3887009.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            35828..>36896
                     /locus_tag="O2458_001139"
     CDS             35828..>36896
                     /locus_tag="O2458_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCZ3887010.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKS"
BASE COUNT        11233 a         6506 c         5512 g        13645 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaaccgtaa agtgttccgc tatttaaatg cctaaatttt aattcatcat ctaatatatc
     2101 cattatcaat tagcaagtat gttttatcaa tcagtaagtt ttactttcgc attatcatct
     2161 aaaaagaacg atttttcata atcatttggt tggtgccatg ttgttttcca cattctattt
     2221 ctattattag caaacattcg ataaccgaaa ttacggactg gtttaggtac aagccataaa
     2281 cctacgccca ataatttagt tcgattaggt agtgctgtga ttagtttaat aatcgcctgc
     2341 gattcaaaat atatacgatc acctttctgt aaaatcacac tatttttatt tgccgcttct
     2401 ggatgttgtt taaaaaattg ttggccaact tcacctttta acgtggcaaa ttgataattt
     2461 cttggtaggt catgttgaat taaccaaatg acatagttat aacaatatat acagtttcca
     2521 tcataatata cgattggcat aatttattcg ctcctttaat agtacttata gtcatttttc
     2581 ctaatttaaa aacatttaaa caaggttgtc acttgataag tcctcgcgac acgaaattgc
     2641 cataaaattt atttttcagt tttatattta ttgtataatg tattttttat ttactttaaa
     2701 tttctcaatc tacagtgtaa tcttatttct tattattaac ttagcgtttg gtcattcgtc
     2761 ttattcaaca taccactgga aaactcatat aaagttttaa ttctgtaaca taaattcttt
     2821 tatattcata tactgtcttt attatttatt aaaataaaaa cgagtaatct atcataagct
     2881 cttaattagc tcttatggtt gattacccgt cattttttct ttatgcggtt attcaaatat
     2941 gcaattatgc aatttttaat cctatcctta cttcttttca aaaactacca tatatcatta
     3001 gtcatgtttg actttgacta tacgtgtgta ttttaaaaac tgctctgaat atttctcctt
     3061 aatttcctct aaactgtcat atgtaatacc tacaatatgc caattaggat tgaaataata
     3121 gaaagaatct ttttgtcgtg accatttaat catattaccg tctttattat aacgtggtaa
     3181 tgttacttca tagccttcta acacattaat atacgtttgg aaaatgtctg tatctatacg
     3241 attaaaatta tcaataacta aataactttt atccttttta ggcattaatt gagtaataaa
     3301 cccttcacga tagtataatg cccctgtttc atttggtaaa tacttgccat aaagcatatc
     3361 ttctgaaaaa tcaggatgac ctgttgttat aacaggattt gcattcgctt tttgcaataa
     3421 gtcattagcc gcttttaagc cttcttcttt tcgatctaca acaagtaaaa taaatggctt
     3481 taacgcttct tctttttcac tttcaagcat atctggtttt tgaaccattt caaatggaga
     3541 tttcaatcca ttattatcgc tcatttcaat aattgcatca tactgtgctt gtgacatact
     3601 tgcaatagcc tgttttgcat tttcttgaag gaaatataag tttttcaatt taggatgctt
     3661 atttaatgta cttaatgtaa tcggtgtaat gtctttctca taagacactt caatcactgt
     3721 actatttgtt ctaccaggaa ttggtggttt ttcatgaata tgctttgata cttctccaat
     3781 tccaacgaca gattgatttt tcgttcgatt ataaaaaata atattgtcgc cttcttctaa
     3841 ctgagtataa aaatgataac cattacgttt aataccgttg tacgtgtgcg tataaatcgt
     3901 atattggttt ccaggttcaa attcttcagt ttcagctaaa aagaaataac gcggtatctt
     3961 aatttcgcct ttaccaagac cacttattaa atcaaactct tctgcagtga tttgattgaa
     4021 caatgtctct ttcatattac ttatacgaaa ttccaaagct tcactacgct ttaaataatc
     4081 tgctgttaat ggtttcaatt gttcattaaa acgaaactgt acacgtattt tattttgtgc
     4141 acctgtttca acactaataa tttcaccaca tccaagtagt ccagtatccg tctgaacttg
     4201 ataaaagatg acttgatctc ctactttagc ctttttaaac gctctaaatc cttgagatgg
     4261 gttaaaatgt gcgcctgatt caaataaagc tgtttgtcct actaacggtt cattatgatt
     4321 ccaacggtta tatccacaat tcaaccaaaa ataattcgtt tctgctgtca tcttaatact
     4381 ccttaacctg aataaatttt agaaacacta tgaattacat tcttttagtg tttcttatgc
     4441 agttggacgc gtatgcgaac aactgtatac cctttgttca ctgcgatttt aatcgcattt
     4501 cctataacat tgtagcgccc aggacattaa tttacgtccc agacccttat cgttttcact
     4561 tctaagtaag tcgaactatt ttgctttaca acaagtgcga ctctaaatac agttggacac
     4621 acatacgagc aactgtatac cttttaatca gttttctata ttttatttat tatatctgtc
     4681 ttaatgataa aaattgttac aaacagttta acatatttag ctacctattt tgcatataaa
     4741 aaacatgctt gcatacacta tgcaataaaa attacaaatt tatatatgat accactatgc
     4801 ttgcttatct ctatagcgcc attgatacac atttttaaat atctatactg ccgttagaat
     4861 tttatcatgt cttaattttc attaaatatt aattacttca ttttatataa accaacaaaa
     4921 accccctcac tactatgcaa gtgagaggtt atgttgatgt gctttatttt catcaatgtc
     4981 attaacacaa tcatatttct tctgttaatg cactgtatta tattcaaacg cgctatcgac
     5041 aattaatttg aagctacaaa ggtaccgcat aatggcagca cattaattaa caatgcgcta
     5101 aactttaatg aggcgctgat ttgcttgtct ttaaaagtca acatagcact gataattcct
     5161 aaaatagcta ctatacgtgg aattgtcatg ctcggatact gcggtatatg taagaaagca
     5221 ccaagacctg ctatcacgcc acatactaac gcaatatata gaaattgctt caaatcattc
     5281 actcctaaat tgttattaca ctattacaca tagctaatca tcaatgtgaa atcaccttca
     5341 aagacactat ccaaatcttc agaagtcaaa ataaagtttg taccagtagt cagtttgaaa
     5401 atttcaccat cgacaatcat ttgcccttcg ccttccaaca ctgtaactaa acagaactct
     5461 ctaggcttca tataatttaa cgtgccagaa atttcccatt taaccaatgt aaagaaatca
     5521 ttcgatacaa tgtgtgtaca cttatggttt tcaataattt cgctttcagg caaaatatta
     5581 ggtaatggtg cattgtactg aataacgtct aaagcttttt caatatttaa cggtctatca
     5641 ttatattgat tatcttgacg attgaaatca taaagtctat atgtaatgtc tgacgattgc
     5701 atcgtttcgt atgctaatat tcctgaagaa atagtatgta ctgttcctgc tggaataaaa
     5761 tagaattctc caggttttac tttaatatat ctaagtatcg actctatcgt tccgtgttga
     5821 acatgattcg caacttcttc tctagactct gctaatgtcc ctataactat ttctgcatct
     5881 tcttctgcat ctataatata ccaacattca gatttgccat attgcccgtt ttcatgctca
     5941 taagcataag aattatcagg gtgcacatga atagaaagtg attctcttgc atccactatt
     6001 ttagttagaa gcggaaaatc tttgcttggg aaatcaccaa acaattcacg atgttctgac
     6061 caaatacggt ctaatgtttg accttgatat ggtccattaa taatctcgct cgtaccattt
     6121 ggatgtgctg acacacacca acattcccca gttgtatcat tgtctaattg atatccaaac
     6181 tcacttagac gttgaccgcc ccataatttt gtttttaaaa ttggttgtaa aaataatggc
     6241 attgttgcac ctccattgtg attaagtaag caatagaact ctgatgttgt tgttccatta
     6301 tattttgatt ttgttctcat ttacatcgta ttattaactt ccacatttca aattaactat
     6361 tagtgattgt accatattta ctaacattgc agtactgcca attaaagcgc ttcacttaaa
     6421 tttacagtac tttaacattt tcaaaaattt atagcataga gattatatct ctcttacatt
     6481 tgtacatatt tccctttaaa tttactcgcc cattatacca attaataaac aactttaata
     6541 gttgtgccat acattgttca aattctttgt aaaacgcata gacaatacgt acttattcat
     6601 acttataatt catcattttc aaaaaataac gagttacgaa aaagtaaccc gcttcaaatc
     6661 atatttacta tccttattaa tccgtttcat tttcaaattg agttaaagca tctttaatgt
     6721 cctgatcacc actaataatt tgaaactctt ggtgattaaa atgattggat gtgacaattt
     6781 cttttaatac tgtcgcaaca tcttctctag gaatttcacc tttaccatca aaatattgtg
     6841 cagcttctat ctttccagat cctgctgcat ttgtaagtgc ccctggatgt aaaattgtat
     6901 aattcaaacc tgaacgtctt aaatagtcat cagcgtaatg tttagctatt gtatatggct
     6961 ttaaatcacc gctatcatca aaagcctgac gtctcgaatc atatgttgaa accatgacat
     7021 agtgtttaat attggcctct ttactcgcaa tcattgattt aacagcacca tctaaatcga
     7081 caataattgt tttatctgca cccgtgttcc ctccagaacc tactgaaaag ataactttat
     7141 cgaatggttt aaacgtctca gttaaagtct ctattgaatc attttcaaca tcaacaagaa
     7201 ttgctttcat accttgtgat tttaacgcat taagttgatc tgattgccta acaccagcag
     7261 taaatggtac attttctttt gctaattgtt gcactagtaa cgaacctaca ccgccattag
     7321 cacctataac caaaatattc atttacaaca ctctcctatt tattattctc tatgccatac
     7381 cactttatga gatatgtaaa acttgttaca actataaaaa tcaattgaca tactactggg
     7441 aacgtattaa attaatatat gaacaaatat tcacatgaaa ggattgtcat ataatgtcag
     7501 aacaatattc agaaataaat acagatacat tagaacgcgt aactgaaatt ttcaaggcat
     7561 taggcgatta caatcgaata cgtatcatgg aattgttatc agtcagcgaa gcaagtgttg
     7621 gtcacatttc acatcaattg aatttatctc aatcaaatgt ctcgcaccaa ttaaaattac
     7681 ttaaaagtgt gcatcttgtg aaagcaaaac gacaaggcca atcaatgatt tattcattag
     7741 atgacatcca cgtagcaact atgttaaagc aagccataca tcacgcgaat catcctaaag
     7801 aaagtgggtt ataatatgtc tcattcacat catcatcatg accatatgca tagtcatgta
     7861 actacaaata ataagaaagt attgtttata tcgtttttaa taatcggtct atatatgttt
     7921 atcgaaatca tcggcggtct ccttgctaac agcttggcat tactatctga cggtatccat
     7981 atgtttagcg acacattctc attaggtgtt gcacttgtcg catttattta tgctgaaaag
     8041 aatgccacaa ctacaaaaac atttggttat aaacgtttcg aagtactcgc agcgttattt
     8101 aacggtgtaa cgctttttgt aataagtatt ttgattgttt ttgaagcgat taaacgtttc
     8161 tttgttcctt ctgaagttca atcaaaagaa atgttaatca ttagtattat cggtttaatt
     8221 gtcaatatcg ttgttgcatt ctttatgttt aaaggcggcg acacttcaca caatttaaat
     8281 atgcgtggtg cttttctaca tgttatcgga gacttattag gttcagttgg cgccattact
     8341 gcagctattt taatttgggc atttggatgg acaatcgccg atcctatcgc aagtatttta
     8401 gtttccgtta ttattttaaa aagtgcttgg ggtatcacaa aatcttcaat taacatttta
     8461 atggaaggca caccaagtga tgttgatata gatgaagtta taactactat taaaaaggat
     8521 tcacgaatac aaagtgtgca tgattgccat gtttggacaa tttcaaatga tatgaatgca
     8581 ttgagttgtc atgttgttgt agaccataca ttgacaatga aagaatgtga attattatta
     8641 gaaaacattg agcatgattt attacattta aatattcacc atatgactat tcaattagaa
     8701 acgcctaatc acaaacatga tgaatcgatt atatgttcag gaacacatag tcattcacat
     8761 aaccatcatg ctcatcatca cgcgcatgta cattaataat tttaacctac tgccattgca
     8821 tcgattaaac ttttcaatgg cagtaggttt tttatgtctt tatggcgact tgtttggtct
     8881 ttgatgatgc aatgtttatt aacaaatttt caactattat ttcttacatt agtcatattt
     8941 ttgacaattt actattataa ttctctaact ttagtcactt taattaattt ttattagata
     9001 ttaatatgaa aataacgtgt tttttgttat tgtatttata tgaagaaaag gaggcaccac
     9061 tttgtttact tactttaaat cagcatttaa aaatgctaaa ccacaattat taataacatt
     9121 gatatatgca cttatagcat ttgctgtaat tgcagttgta tatttacttg ctaatttcca
     9181 gcttgctaag tatgcacaaa ctattgcaat ttattcacaa tttgggcaaa aaccaccagt
     9241 agatgcatat ttaaaagtta tcgctgtatt acttatagca gctgtagttt cactgtttgt
     9301 tttagttcaa atttttatcg gaattacaaa tgttatgaaa cgagcaatga gtcatgaaaa
     9361 agtaaaattt accgatttat ttattgcatt taaaaaaggt aattatttaa agagcgttct
     9421 gatcggtctt gtatctatag caatgattat cgttctttca cttctgacat cattactata
     9481 caaattattt tctccagtat cagaaatgat tatgaattca gtacaatcat catatgcaga
     9541 tagtacacac ctgattggta ttgctattac aactcaaagc atcataatca ttgttgtttt
     9601 attgattaaa gcaattatta cttggttatt gttaatacct attttcaact ttatgactag
     9661 tttcgttgaa tcaactaacg ataaagtaaa aacacactta gctaatggat ttaaagcaat
     9721 gaaaaatggt caaaaaacat ttttcaaatt tttcattgga atactattat taaacttaat
     9781 tattatttta tttaaaacac ctgttggtta cttgatttca tttaatacgc aatcactctc
     9841 tcaaagtgtt gcagaaaata tcataagagt ttatacagta atgacaatta ttttattcgt
     9901 tgttattcat gcaatcattt taatgggtat cgttcaatat tacttaaaac gtggtcaaaa
     9961 aattactaaa gataaagtaa aaacagctga caagaataaa aaagttgtca ctgaaccaaa
    10021 aaatacaaaa gtagaaaatg ataaagtgac aacatctgtt gaaacaaaaa cagaaaaagc
    10081 acaagattca ttaaatgata atacaacaaa aacaatgact tcagataaac cagaagacaa
    10141 tcaaccaaaa taaattttag agccccttac aactttagtt gttaggggct cttatgcatg
    10201 cgcttagcgc aggcgctcct acttttaaac tttagttgtt aggggctctt atgcagttga
    10261 tgcgtagcat ccaactgtgt tcctctattt ttcaacttta gttgttaggg gcttctttct
    10321 aaaataacta agtgaattat ctatatatga agaggtctcc aatacttttt atataattcg
    10381 tgattacatt ctatactttt aattgtattt tctgctgtat ggaaagttgg ttttattaat
    10441 tctgattatg ttcagaacta caattcataa gaaaaatgtg ttaaaatata tttgtagtaa
    10501 gtagaagcaa aagatgaaaa tcattaactc ttgaaacaca aaaagggcaa cactcggaaa
    10561 catgttaccc taatgagccc gttaaaaaga cggtgacctt atattttatt taaaaatagc
    10621 cttcaaaaat gccggtcaaa gcgaatagaa ggttattttt tatggtcttg agtactaatc
    10681 aatactaaac caatgattgt taatacaacc ataagcattt caaaatcaga catgctagtc
    10741 ctttctagga gtaacaacta taaccatagg catcacccct ttattaagag attagccacc
    10801 atctatccaa cttgctcaat aaatattata acatgcacac ttttaaacag taattcatag
    10861 tataagtttt ttcatttctt aatcaactcg ataaacagtt gagtgacata cccttgtcta
    10921 actagctatt tcttcccctg ttatcgtttg ttcatcatat aaaaatttaa tttctcattt
    10981 tttattctct ggtaaagagg ggcgcccgct ttttcggcct ctcgctcttg ttgctgctaa
    11041 gtctatttta tacactcaca tagtaaatta acttctaatc ctgaaaaatg cactcatcat
    11101 cgtaaaaaac attttgccca ttgtgtcttt cgttgaaatg ttcatgttac taatttgtaa
    11161 gtcaattccg ttatcatcga atcattgtga taattcaatt aatagttttg tcgttctacc
    11221 taaccgatca agttgataga caactaatgt gtcgctctct cgtaaataat ctaaacactt
    11281 aacaagctct gtacgtttga caccacttat tctttcacta tatacacacc cacacccaaa
    11341 ttctttaagt gtatcgatat taatctttcg ttgatacacg atcataacca attttagcca
    11401 tttaaataaa acctctcttc aacatattat atttagttat gactcaaaac tcattgccgt
    11461 atttttgatt ttgtattaat atatcaaggc tatcatcatc taataccaaa tgaatttctc
    11521 cacttcgaat atcaatagca acgtttattt ttgattctaa ctttttcaaa taatctatgt
    11581 cagtaaatat gtacttagaa agataatcat cttctttttt aaattcttct ttatcaattg
    11641 tatatccgtt ctctaaaatt tttgataact ctataagagt attactccta ctttcttttt
    11701 ttacattaga cactacatag tttatgaact tttcaatcaa ttgttcttcc aaatcactat
    11761 aatcttgtgt gtccttttta aataattcaa acataatatt attggataaa ttttcattat
    11821 atattaatcg ctcgtacata ccagaagcgc tagagttgat aaatctattc agatcgtttt
    11881 gtatttcgtt caaatctata ttccctttac tatcgtaata tgaataatta ctcattaatt
    11941 tttttaagaa atctctcaaa ccaaagtaaa gataaatatt tgaatacttt ctggcattat
    12001 cgtagtcttt tacatctctt ttatagtgat ctatcaacat attcaattcg aaattatatc
    12061 catgattaaa atcaaactcg ccattgtcat attttctttg aaattcaaac ttgtatattt
    12121 ctgcaacttt atcatcgcaa ttttcatcta tatagttttc aaccaaataa taagcacgct
    12181 caagagaaat ttcaccataa ctttgtataa aagaggttaa gtactctgtt ttctttaaaa
    12241 tttcttctcc tttcttagtt ggtatatata catatggtaa atctaagtta ttaataatat
    12301 gaaaattgtc gtcaatacgt ttaattaagt caggtttatt acctgttgtt tttataccaa
    12361 aactttttaa aatactttgt aattcaggtc ttgtcttttt atatatacta acttcaaaat
    12421 cattagtcgt tgttaataac tcagcttcaa ttaatttttt tattgaattc ataatttgaa
    12481 catcattatt agcaaggcac ttattagcaa atatctcttc tccaaccaat ctatttacat
    12541 tagtgtgtaa tactaataaa tcacgtgcat ttacgctcac tagctttcat ctcctcattt
    12601 ttgcttttca aatttattaa gtatgaataa aaaatacatc cgaacaaaca aaatctctat
    12661 attctaattc gatttacata gattatacac taaaaaaatg attctcaatg caaatgaatg
    12721 tgtctctcat agtctaaaaa ctcgtatgtt tcgaatttta tgattcattg ataatagtac
    12781 cagttaaaag aactaagatt gtaattatcc ccccttaaaa ttcaagaata tattttctta
    12841 atctatcagc aagaggttat atttcaaagt gtttattttt aaaacaacat ctaaaagcca
    12901 ttttcataat aatgttaaat cattagaatg tataaagatt ccaattaata aaaatagata
    12961 aaatgcaata aagttccaaa tacttattat tattcatcac aaattacgtg atgccctcta
    13021 caacactaaa tcaaggatga tatttatttt atatacatta gcattcattg tttgcttacc
    13081 cacattatcc tgtttagtct tttttataac tctagtcttg aatgtcatat ttacaaagaa
    13141 aaaaacacta aaatatttaa aactaacact atttattcac tttgttcttt tatagcacct
    13201 tttatgttta ttttcttgta ttcaataaat agtatggaga tgataattta aagtagatat
    13261 atttgaagta tactatagag taaattatat tggggaaata tgtaaacgaa ccggttgatt
    13321 ttatagtgga ttaacacttc atagagaata taatcaagaa caaaaacagt caataggtgt
    13381 gaattttttt aaagacggat ctgttgctac taaaaaaaga agtatacaat tcaatagtta
    13441 aaccttaaaa caagaaatat tattcaaaat caatgaattt cctatcttat tagttttaac
    13501 aatatttatt ctcatagagt ttgtccaatt aaatatagat gattcaaata tttataagca
    13561 tacaaaagag cagtaagaca ttttctaata gaaaataact ttactgctct ttgtacatta
    13621 ctcatcaact attgtaaatt aaataataaa taactaccta ttttattatt cagctaaaaa
    13681 atgctctgct aaatattttg ctgcgccatc ctcttcattc gtgtatgccg ttacatctga
    13741 agttaatgct tggatttcag ggcgtgcatt tttcatagca actgtataat gaccaaattc
    13801 aaacattgct ctatcattgt cgctatctcc aataactaac gtttcttctt gatgaatacc
    13861 aaaatgtgca atcatttctt taatgcctgt acctttatca gtttgataag ccattgtttc
    13921 cgcattaaat cttgatgaat ttgaaacact aatctgtagt tgcacatgat tttgctttaa
    13981 ttcatctcta aatgctgtta ttttttctaa attagaactg aatagataaa tttttgaata
    14041 ttcaccttca ggaaacttag ttacccaatc tatcttacca gcaagcgcat cttgtcttga
    14101 agaccattca ctatgactta cgccattaat aggatcttga ctacgaatca tatctcgcat
    14161 ccatgtttca tcttctttta aagaaactct attaccttca aaaggaaata cctcataata
    14221 aatttgttgg cgcttagcta aattagtaat ttgttgcact tgagccaatg ataaaccatg
    14281 cttgaagata atttctccat ctacttctcc aattgttcca tttgaactaa tgatgccatt
    14341 aaccgcaaaa tcttgaggta caagttgatg tatttcagaa tgcgaacgtc ctgttgccaa
    14401 aaatacttta tatcctttct ctctcaattg attaatgaca tctttcgtat acgttgatgc
    14461 ttgattattt tcatgtaaaa ttgttccatc catgtccaaa aatattgctt ttacgttatc
    14521 catattgccc tccactgtcc attttatctt aattaaattt tgtcattatt acttattatt
    14581 tgcaaaatat ttactctaac attgccatag aaaatcgatt attccatctt tgaactgcta
    14641 catttttctg gaaaaatcgt gacatgtttt cagaagttaa tatgtcttct acagcgcctt
    14701 gttgaatact ttggccatct tttagcagta aaattttgga aaagttagca gttatttctt
    14761 caataaagtg cgtcacataa atcatcgcaa gcgttggata tgaatctgac aatgagtcaa
    14821 gtatacttaa caacgattct cgtgcaataa agtctaaacc agctgctggc tcatctaaaa
    14881 ttaaaacctg gggttgcccc attaaagctc gtgcaatcat cactcgttgt ttttcaccgg
    14941 tagataaata accaatatat tgttgcgctt tagcaaacat tccaactaat ttaagtaatt
    15001 gatgtgcttc attacgtatc tcatcatcaa tatcttgata aacaccaatt gatttaaagg
    15061 caccgcttat caccacatcg attactcttt caccctcttg aaacttttcc agtaaactat
    15121 gagatacaaa acctatatgt tgtcgtacag tctctgcaga ataccctacc ttgcctggca
    15181 ttttaccgaa aaggttaaca gttccagatg ttgcaggctc atacgcattt aaaatattta
    15241 gaagtgttgt cttgccagca ccattcaacc catataatat ccatttatca cctttagcaa
    15301 tttgccaaga aatctttttt aaaattgttt ttccttgctt cattcgccca atttgatcta
    15361 attgaattaa catgcgaggt caccccttta ttgctactcc atacttctca taaaatcata
    15421 ttaacataac acccttaatt gtcagactat ttaaataaat aaaacacttc atttttacgc
    15481 atttctgcca aattaagatg aagtaaaagc taagtcgacc taaaaaagca cccttctagt
    15541 cgattaatct aaaagggtgc catatacttt aattttaata catgattgat tctaaaaaag
    15601 tgaattattc cacagtaact gatttagcaa ggttacgtgg tttatcaaca tctaaatctc
    15661 tgtgtaatgc tgcatagtat gaaattaatt gtaatgcaac cactgatact aatggcgtta
    15721 acaattcatg tacatgagga atgacataag tgtcgccttc tttttcaaga ccctccatag
    15781 aaataataca tggatgtgca ccacgtgcta ctacctcttt aacgttacca cgaattgata
    15841 aattaacttt ctcttgtgtt gctaaaccta caactggtgt accttcttcg attaaggcaa
    15901 ttgtaccatg tttaagttct ccaccagcaa aaccttctgc ttgaatgtaa gaaatttctt
    15961 taagttttaa cgcaccttct aaacttacgt tatagtcaat agtacgtccg ataaagaatg
    16021 cattgcgtgt tgtttctaag aaatctgtag caatttgttc cataattggt gcatcgtcaa
    16081 caattgcttc tattgctgtt gttacttttg ctaattctct caataaatca atatctgctt
    16141 cacgaccatg ctcttttgca acgatttgag acaagattga taatactgca atttgtgcag
    16201 tatatgcttt tgtagatgca actgcgattt caggacccgc gtgtaataac aatgtgtggt
    16261 ctgcttcacg tgataaagtt gaacctgcaa cattagtgat tgttaatgat ttatgaccta
    16321 atttattagt ttcaactaat acggcgcggc tatctgcagt ttcacctgat tgagaaatat
    16381 aaacgaacaa tggtttttca gataataatg gcatgttgta gacaaactct gatgcaacgt
    16441 gtacttcagt tggtacgcca gcccattttt ctaaaaattc tttacctact aaacctgcat
    16501 ggtagcttgt acctgctgca ataacgtaaa tgcggtctgc ttctttaaca tcattgatga
    16561 tgtcttgatc aattttcaag ttaccttctg catcttgata ttcttgaata atacgacgca
    16621 ttactgctgg ttgttcatga atttctttta acatgtagtg tgcataaaca cctttttcag
    16681 catctgatgc atcaatttca gcaatatatg aatcacgttc tacaacgttt ccatctgcat
    16741 ctttaataat aacttcatct tttttaacaa taacgatttc atggtcatgg atttctttat
    16801 attcgcttgt cacttgtaac attgcaagtg cgtctgatgc gataacattg aaaccttcac
    16861 caacacctaa taataatggt gatttatttt tagcaacata gattgtgtct ttgtcttcag
    16921 catctaataa acctaatgca tatgaaccat gtaataatga cacaactttt gtaaatgctt
    16981 cttcagttga aagtccttga tttgaaaagt attcaactaa ttgaacgata acttctgtat
    17041 ctgtttctga aatgaatgat acaccttgta agtattcacc ttttaactct tcatagtttt
    17101 caataacacc gttatgaact agagtaaaac ggccatttga tgattgatgt ggatgagagt
    17161 tttcatgatt cggtacaccg tgtgttgccc aacgtgtgtg accgattcca acaggtccat
    17221 caaaatcgct actatcagca actttacgta attctgcaat acgacctttt tctttaaata
    17281 cagttgtatt atcatcattt actactgcga tacctgcaga gtcataacct ctgtattcta
    17341 atttttctaa accttttaat aataattctt tggcattatc atagccaata taaccaacaa
    17401 ttccacacat aacgacattt tcctccatat tggaatagta cgcgtaaatt atgatttatt
    17461 gccgataatt tagattgaca atctgctttc ataatataaa taggaacatg ctatcatcgc
    17521 attcatccat aacaaattaa gcatagttat ttttacaact atacaaattg ctcacactgt
    17581 actttccata ttaatatttt ttatattcaa tttctggcga tcttattaac tttgtccatt
    17641 aagtcaccct aatgttttac ttaataagct aacgaatgag ccacatccgg gatagcatcc
    17701 gccgatctat tcgatcacta tcctcctcgt ctacaaatac atatattgca ctctataaag
    17761 gccactcata tattaacctt taatcttcaa atacaaatat ttatttgcac aggcgcttta
    17821 actgtactgc cgaactttcc ccctttccat taatcattat tgtacaacgg tgttgttttg
    17881 ttttgcaaat attttcacaa taaaatttta aaaatcctaa aacaattttt ttgttttact
    17941 ttttcaaaat atctatactg tcacattgat gacactttat ttaattttgt cacatttatt
    18001 ttgacaaagt tgatttttgt ttatattgag taacaagtaa cctctctata cactatatat
    18061 agtcacatat attaaaaaag aggtgtaaac atgtcacaaa ctgaagagaa aaaaggaatt
    18121 ggtcgtcgtg ttcaagcatt tggatcattc ttaagtagta tgatcatgcc aaacattggc
    18181 gctttcatcg cttggggttt tatcgcggcc atttttatag ataatggatg gttacctaac
    18241 aaagatttag ctactttagc aggaccaatg attacttatt taatcccatt attgatcgca
    18301 tttagtggtg gtcgtttaat ttatgattta cgtggtggta tcatcgcagc aacagcaact
    18361 atgggggtca tcgttgcatt acctgataca ccaatgttac ttggtgcaat gattatgggt
    18421 ccacttgttg gttggttaat gaagaagacc gaccaattga ttcaacctag aacgccgcaa
    18481 ggttttgaaa tgttatttaa taacttttct gctggtattt taggatttat catgacgatt
    18541 gcaggattta aaattttagc accacttatg aaatttatta tgcatatttt atcagtggca
    18601 gttgaagcat tagtacatgc acacctactt ccacttgtaa gtattttagt agagcctgca
    18661 aaaattgtat ttttaaacaa tgcgattaac catggtgtat tcacaccgct tggtgcagat
    18721 caagctgcaa aagctggtca atcaatttta tacacaattg aatctaaccc tggaccaggt
    18781 ttaggtatct tacttgctta tatgattttt ggaaaaggca ctgcaaaagc aacgtcatat
    18841 ggtgctggaa ttatccactt cttaggtggt attcatgaaa tttatttccc atatgtatta
    18901 atgcgtcctt tattatttat cgctgttatt ttaggtggaa tgactggtgt tgcaacttat
    18961 caagcaacag gatttggatt taaaagtccc gcgtcaccag gttcatttat agtttactgt
    19021 ttaaatgcac ctagaggtga attcttgcac atgttactcg gtgtcttcct tgctgcactt
    19081 gtatcattcg ttgtagctgc tttaattatg aagttcacta gagaaccaaa gcaggattta
    19141 gaagctgcga cagctcaaat ggaaaatact aaagggaaaa aatcaagcgt tgcttctaag
    19201 ttagtatctt ctgataaaaa tgttaataca gaagaaaatg ctagtggtaa tgttagtgaa
    19261 acatcttcat cagatgatga tcctgaagcg ctattggata attacaacac tgaagatgtt
    19321 gatgcacaca attacaataa tataaatcat gttatttttg cctgcgatgc gggtatgggt
    19381 tctagtgcaa tgggtgcaag catgttacgt aataaattta aaaaggcggg cattaatgat
    19441 attacagtta caaatactgc gattaatcaa ttgccaaaag atgctcaatt agttatcact
    19501 cagaaaaaac taactgatcg tgctattaaa caaacaccaa atgccatcca tatttcagtg
    19561 gataatttcc ttaattcacc aagatatgaa gaacttttaa ataatctaaa aaaagatgat
    19621 caagcataat aattaaataa attaaaaaat ggaggatacc gccatgttat tgagtacacg
    19681 tgaaaaagaa atgatagccc tattgattaa gtaccacggt caatatatca ctatacacga
    19741 cattgctcag caacttgcgg tgtcctctcg tactattcac cgtgaattaa aaggtgttga
    19801 agcatattta acttcatttt cattaacttt agaacgcgca aacaaaaaag ggctacgcat
    19861 tgctggcaca gattctgatt taaacgattt gaagcaatcg attgcacaac atcaaaccat
    19921 tgacttatct gttgaagagc agaaagtaat tattatatac gctttgatac aagccaagga
    19981 gccagttaaa caatatagtt tagcgcaaga aattggcgtt tctgtccaga ctttagcaaa
    20041 gatgttagat gatttagagc ttgatttaaa taagtaccaa ctatctttat ctcgaaagcg
    20101 tggcgaaggc atttacttgg taggtactga atcaaagaaa cgtgaatttt taagtcaatt
    20161 aatggtgaat aacttaaata gtactagcgt ttattcagta attgaaaatc attttgtctt
    20221 tcattcatta aatcaaatcc acaaagactt tgttgactta gagcgcattt ttaatgttga
    20281 aagactatta atggactacc taagtgcctt accctaccaa cttaccgaat caagttattt
    20341 aactttaact gtccatatcg tgctctccat ttcacgtata aaaaatggag agtatgtcgc
    20401 attaaacgat gatatttatg attctgtaca aaacacattt gaacacaaag tagcaagcga
    20461 acttgctgat aaacttggtc aaatatatga cgtcacgttt aatcaggcag aaattgcttt
    20521 cattactatc catttacgtg gagctaaacg aaaaaatctt aatgatacat cattaaataa
    20581 tcgttgtgaa gaaaacaaaa ttaaagcgtt tgttaacaaa gtagaaatga tttccggtat
    20641 gacatttgca gatttggata ctttagtaga tggactgacg ctacacctta atcctgcaat
    20701 caatcgtttg caagctaata tcgagaccta taatccgtta acagacatga ttaagttcaa
    20761 atatccaaga ctatttgaaa atgtaagatt agctttaaat gattgttggc ctgatttgat
    20821 ttttccagag agtgaaattg cttttatagt tttacacttt ggtggctcga ttaaaaacca
    20881 aggtaatcga tttttaaaca tattagtcgt ttgcagcagt ggtatgggaa ctagtcgtct
    20941 attatcaact cgtctagatc aagtttttag tgagattgag cgtattacac aagcatcagt
    21001 cagcgatttg aagtcactag atttaagtca atatgatggc attatttcta ctgtgaattt
    21061 agacatcgac tccccctatt taacggtaaa cccattatta ccagatagtg atatcagtta
    21121 tgtcgcacag tttttaaata caaagtctac gttccaagag acgcatgata aatcatcaaa
    21181 catgattgat aaggatgatg ttcatgttga aacgaaagat gttgatggca acacatcttt
    21241 tgaaaatgaa caaacttcat acttaacttc agttttcgaa aaacatttaa gtgacgaaaa
    21301 atcagaacaa ttattgcatc atatgcgttc gggtttaact ttgcttgatt cagtgaaaat
    21361 agttagtacc gaagttaaac agtggcaaac atatatcgca gattatctat atcaatgcga
    21421 tgtaataaac gatccaacgt cattcgctga actactagag caacgattga ttgacaatcc
    21481 aggatggata ttaagtccat atcctgttgc aataccacac ctaagagaca atatgattaa
    21541 acaccctatg attctaatca cagttttaga agaaccgtta acattgccta gtattcaaaa
    21601 tgacaatcaa acaattaaat atatgatttc catgtttatt tctgacaatg attttatggc
    21661 atcactggta agtgacttgt ccgaattttt aagtttgaaa ttagaatcta ttgatacttt
    21721 tatggaaaat ccacaggaac ttgaaacatt attaagaaac aaatttttag aacgaattaa
    21781 aaaacaattt atttaggagt gtacaaaatg agcgaattat ttagtaatga caatatcttt
    21841 ttaaatgtaa atgttaacag ccaaaatgaa gcaattgaaa aagcaggtaa agccttagtt
    21901 gatagtggtg ctgtaacaga tgcttatatt caagcaatga aagatcgtga gcaagtcgta
    21961 tcaacattta tgggaaatgg cttagcaatt cctcatggca cagatgaagc taaaacaaat
    22021 gtgattcact caggtttaac attattacaa atccctgaag gcgttgactg ggatggcgaa
    22081 gtagttaaag ttgtcgtggg aattgctggt aaagatggcg aacatttaga cttgttatct
    22141 aaaattgcaa ttacatttag cgaagaagaa aatgtggatc gtatcgttca agcaaaatct
    22201 gcagaagaaa ttaaacaagt attcgaggag gcagatgcat aatgaaagca gttcactttg
    22261 gtgctggtaa cataggtcgt ggtttcattg gttatattct tgcagacaac aatgttaaag
    22321 taacatttgc agacgtcaat gaagaaatca ttaatgcttt agctcatgat catcaatacg
    22381 atgttatttt agctgatgag tctaaaacaa cgacgcgcgt gaataatgtt gatgcaatta
    22441 attcaatgca accttctgaa gcgttgaaac aagcaattct agaagctgat attattacaa
    22501 cagctgttgg tgttaacata ctacctatta ttgctaaatc ttttgcgcct ttcttaaaag
    22561 aaaaaacaaa ccatgttaat attgttgctt gtgagaatgc tattatggca actgatacat
    22621 tgaaaaaagc agtacttgat attactggcc ctcttggtaa caatattcat tttgctaact
    22681 cagcagttga tagaattgta ccattacaaa agaatgaaaa tatattagac gttatggttg
    22741 agccatttta cgaatgggtt gttgaaaaag atgcatggta tggtccagaa ctaaaccata
    22801 ttaaatatgt tgatgattta acaccatata ttgagcgtaa attattaact gtgaatacag
    22861 gacatgcata tttagcgtat gctggtaaat ttgcaggtaa agctacagtt ttagatgcag
    22921 ttgaagatag ttcaattgaa gctggcttac gccgtgtttt agctgaaact agtcaatata
    22981 ttactaatga atttgatttt actgaagcgg aacaagctgc ttatgttgaa aaaataatag
    23041 atcgtttcaa caattcttat ttatctgatg aagtaacacg tgtcggacga ggtacattac
    23101 gtaaaattgg ccctaaagat agaattataa aaccattaac atatctttat aataaagatt
    23161 tagaacgcac tggtttatta aatacagctg cattgttatt gaagtatgat gatacagcag
    23221 accaagaaac tgttgagaaa aataattaca ttaaagaaca cggtttaaaa gcgtttttaa
    23281 gtgaatatgc taaagttgac gatggcttag ccgatgaaat aattgaagcg tacaattcac
    23341 tttcataatt tattgagctt tgtttgaaac aagaagtttc caacgttatt cgttaacaat
    23401 cagtaataat gtagtagttc ccttgaatta acaatattaa atttctgaac ataaaaaata
    23461 ctcccttcaa catagacact taacttgtgt tatgtatgaa aggagtattt ttgcgttaat
    23521 aatttgtttt attttcgagc cacagccacc tattcaatgg ctattggtca ttactaaaac
    23581 aaattcatat taactgttag acttggttac ttagtaagga atatttccct atgaaataac
    23641 tagatgttca cattcttgaa taaattttat tcttcagttt gttggtcttt cttagtgaat
    23701 cttctaatta agaatgccat acctgcacct agagctaatt cagcatatgg taaatcgtca
    23761 ttatgtgaca taccagtatc tggtaaagtt ttagcttgtt gtttagcttt attaactttt
    23821 ccttgttgag ctgattttgt cttagcttgg tggtcgtcag tgttagttac attaagcata
    23881 tcttgattag cactattgct tccatttgaa actgtagctg gagatgcatt ggcaccgtcg
    23941 ttttgcgtag ctttattgtt tgcagctgaa ccaactgatt tttgcgtatc attagtatct
    24001 gctgttgccg tatcatcttt ttggctaaca ttagttgaag tcattttttc ttttgcttca
    24061 gaagatgcag atgttgatgg tttattcgaa acttcagtat cagctttgct tggcgattta
    24121 tctgcttcgt tagatgcaac gttagtttca gacttaagtt gtcctgcatc agtttgattt
    24181 gtcgtacttt cttctttatc ttttgatgta ttagaaggta catttggttc tgttatgtct
    24241 gctgaaggca atgtttcagt tgttgattca accatacttt gatttgttga atcactacca
    24301 tctttttctg ccttagcttt attttcagat tttggttgtg caaccttgtc attagttgat
    24361 tgagattcag cactattatt tacttcagca ttttgttttg aatcatttac agatgcatta
    24421 tctttgctat cagcagatga tgctgcttct gtgctcgcag ttgttggagc cgttgctgtt
    24481 gatcctgttg gtgcattctc gtttgttgct gtagttgtac tattgttatt tgttgtgctt
    24541 tctgctggcg ttgcattatc agtttctgtt acaggtttat cagttgtgcc gttattagtt
    24601 gattctactt ctggtttact agttacatcg ttatccattg tcggactgtt tgttgatgca
    24661 tctacactag aattgttatt agcttgcggt ttatcatttg catcatcagt tgctgatgtt
    24721 gctgttgttt cacctgttgc cgcatcacta ttatttggtg ttgtcggaga agcgtctgct
    24781 ttgccattag ctgtcgtctc agatacgtta ggttgtccag tattttctgg tgttgcatta
    24841 gcatttgaat ttgctgttgc atcattatta tctataccat tattagtatc attagcatct
    24901 ggatcattct gaggcacaat cgcttcaatt gcaggtatcg ttacattttg taattcagca
    24961 acttctgcat ttgtttgtgt tttatctaat ttatcagcaa atctgtcaaa atatctacct
    25021 aaatccgtac gtgcaatttc tttcgccgat gcatctgcat ctgcattttt aattatttct
    25081 atttgcttgt taaccacttc tctgattgct tccaaagcat ttttcttaac ttcaggatta
    25141 atacgttgtg ctttaagttg ttcaagcgca ctatttttga cagtagcgat ttctgcattt
    25201 gtagtttgat cagaaatatc ttcagttgct tttgataaaa tgtcttctaa agcattcgta
    25261 aacgcttctt tttcttcagt tgtagcatca gcgttgacat ttacacctgc ttcaatctgg
    25321 tctagtgcag tttctaattc ttcgatagct ttttgttttt ctgttgagtc gatttgaatg
    25381 ttatcaaatg cttcaagtcc ttgagctttc gctttttcaa cttcagcagt tgttgttgca
    25441 tcagtaatac cttgtttagc ttgatctgta atttgtttaa tcattgctaa tgcttcagtt
    25501 ttttcttcag cagttaactg gtcactttga tcaatagatt ctttcgtatc ttctgcttta
    25561 acttcaatag cttggttcgc tttaggttta acagtagcat ctacttgaat agcatcaatt
    25621 gctgctttac cttgtgttaa tgcatcatca acgtcaccat tatccacacc attattaatg
    25681 ctttctaatg cagtttgaac attttggtca acttgcttaa ttgcttgttg cttttcatct
    25741 tgtgttgcat tagtgtcagc tgaaatatta tttttcttct gatctgcata agcatataaa
    25801 tctgttgtag ctgattcttt tttacctgtt ggaattgtgt aatcgttaat attatctaag
    25861 tcattatgaa tttgaacttc aatgtcatct ttagaagtag cttgattaac attttgatcc
    25921 gcagtttgtt ttaattcagc aagtttttgt ttcgcttcag caatttcact tgaagtcgat
    25981 gcgttagagt tatccgcttc gtttacttta gcattatatg catcttcaat tttagctaaa
    26041 gcatcttttt tgtactcact aaatgtttta actgcattaa ttttagcttt tccttcttta
    26101 actgcattat caacatattc atttgttgat gattgatcga catttgtttt cgcttgattt
    26161 aattcagtat caacttcttg ttttgcatca ttaatttctt gttgtgatgc atttggtgtt
    26221 tgttctattt gtgttttctt atcagctgca gcttgatcta attctttttt acctgctggt
    26281 ttcttaacag gatttgcatg aagttgttga actttttgta ctgctgtatc tttagcagta
    26341 gttacatcac ctgtagtagt tgctgcatta atattattta aaccttcttc atatgctgct
    26401 ctaactggtc caatatcgtt acctttttct tcattagtag tctcattatt attaagtatt
    26461 tcagttattt tattttgcat ttcagttagc aattccgctc ttgcattcgt tttaacatct
    26521 gttgatgctt gaattgggtc aattgcttga attgcattat cttttgcagt gtttacatca
    26581 tcgattgact gtgcattttc aatattttga ttaccttgtg ttaattgtgc gtctacttgt
    26641 tgatttgctt gttctttttc ttcagtagtc gcatctgcag tttgtgcaat aagcgctttt
    26701 tgttcgtttg cttttgttgc taattcatct ttcgcaacat ctttaattgt tgtatctgca
    26761 gtaatacctt gaatatcagc aactgcttga tctttaattt gcgtaacatc attagttgtt
    26821 tgtgcattta agatatcttg atacgctttt tctttagctt ttaaaactaa atcttttgct
    26881 gcatttttct cttcagttgt agcaccagtt gtattatcaa ttgcttgatt ttgagttgtc
    26941 acagcttgat caacatcatt tttagcattt gatttaaccg ctgttgctgg ttgcgtgctt
    27001 tgaattgaat tctttccagc gtctttcgcc tgatctacac cattatcatc agttgcagct
    27061 gtaatattat ttttcgcgtc tgtaactgct gttgctaatt gttgaatagc tgcttctttt
    27121 tcttctgttg tagcgttctg atcattattg ataacatttg tttgcgttgc ttgtaattga
    27181 tcaatttcat ctttagccgc ttgtttcttc acaacttttg gtgttaccgc attaatcgct
    27241 gcttctgcat ttgctttagc ttcatcaact tgtgcgttag tagttgctgc tgaaatggct
    27301 tgatttgctt taccattttc agtatttgct tcagcatcag ctgcttgttt ttcttcatct
    27361 gttgcatctg gcgtagcttg aatctcttgc aatttgttat ttaaaattgc tgtgatttca
    27421 ttacgtgcag ttgctttttt attaactgtt ggtgttactt gatcaatact attttcacct
    27481 gtcgttttcg cttggtctac atcattttga ctattagcag cttcaatgtt gctatttgct
    27541 tgtgtcacag cattatctac gtccgcatta gccgctgcaa tttcttcagc agtaatgtct
    27601 tgcgtttgag cgattgctgt tttacgttca ttcgctttcg tagcaattgc ttctttcgca
    27661 ttatctttag ttgttgttgc tggctgaatc gcttcaattt tagcaattgc tgctttttta
    27721 gccgcttcaa cttccgcatt tgtatgtgct gcatctattg cggcatcagc tgttgttttt
    27781 tcagtttgaa cttgttattt agcagctgct ttttcttcag ttgttgagcc gttatttcca
    27841 tcaattgctg tttcttgagc ttgtacttta tctgcaattg cttgttttgc tgctggttta
    27901 acatttgcat caggtgtaat ggctgcgatt gtagcttcat ttgtagtttt tgcattatcc
    27961 acatcattgt ttgctgcagc attatctata tcagcgtttg cagtaactac tgcttaatcc
    28021 actttgtctt tcgctgcttg ttgttcttca gtagtcgaat cattcattgc ttcaattgct
    28081 gttttacgtt cacttgcttt ttgagcgatt tccgcttttg catccgattt cttagttgtg
    28141 gcagcttgaa cttgattaat tgcagcaata ctattgtctt tagctgttgt tacatcactg
    28201 tttgtatttg cagcatcaag atttgttctt gcttcttgct ttttagtatc taattctgta
    28261 tatgcagctt gtttttcttc tgtagttgaa gcattgctat tttgaatttc ttgtttacgt
    28321 gtattatatg cgttttctac ttccgtatca gctgcaggtt taacttttgc ttgtgtttga
    28381 attgcattga ttttatctaa tacttttgat tttgtatcag caacttcctg ttttgattta
    28441 acaacttgga tgtcatgtaa accttgcttt tgagctgcat ctactgctgc atcagcttct
    28501 gctacttctt catttgttgc atttgaaact gtagcatttt gagcttttct agctgttgca
    28561 gcttgtttaa cttcattata ggcatccatt tttgtttgcg tagagtcagt aatattttca
    28621 atttttgaga tttcttcgtt tttaactctt tctaaatctt gtgcagtcgt tgtcgcttca
    28681 attgctttaa caccagaaac tttagctgca ttaatacgtt caatagcttc cgctacttct
    28741 tcatttgttg tatcaggatt taaaggtgct tgatcaattt gtgcttgaac aacttcgtca
    28801 aattcttcaa gagctgctgc tttagctgtt gtagcaggtg tagtttgatt aatatcattg
    28861 attgcttttg ttttaatggc ttctacttgt gcatttgttg ttgctgcatc aatatcttta
    28921 attgcctttg tttcaatttt accaatttta tcatttgcta catctttttc atcttgtaat
    28981 gatgcatttg actttttaat ttgttgttta cgagttgtaa ctgcttggat aatatcttgt
    29041 ttcgcttgag gtttaactgt tggtgtaatc acatcttgtt ctaacactgc gataccatta
    29101 tctttttcag ttgtgacacc ttgtgctgta gtttgtttat taatttcagc gattgcatta
    29161 tttttatcat tgttaatttt agttacaagt gctgcaactt catcttgcgt aactttttta
    29221 ctttgttgtg ctgctgtaac cttttcttga gctttagtat ctaattcagc aattgctgct
    29281 tgattatcaa ttaatgcagc ttgtaattta gttactaaac catcaatatc cgcttgagat
    29341 gcacgatttg cagttttaac atgattcgca tcttcattta aaatagtatc tgcttgttgt
    29401 ttaagtttat tgtattctgc aattgatgct gttgtgtaat gactattatc aacttgtgag
    29461 tttacttgtt gttgcaacgc atctttgttc atttcaaccg ctactgaaaa tggatctgca
    29521 gttagtgtaa cttctggttg cgcattatta attacaacat ctgaagcagt acgatatgtt
    29581 aatttttcat taaaatcaat atttttaggt gtatcgatat tcgcaacatt aactttatat
    29641 gataatttta aagatttatc tgggaataaa acttctttag tgtgtgtacc acgtgccgtt
    29701 gtcacacctt ggcttgtaaa tgtaacttta tttgcatttt gatcataatt aacagtcata
    29761 tttttcaata ctgtactgtc ttcattacca ttagggaatg ttgtagttaa tgaattatta
    29821 acgtaagtta caccttgtgg taatgttact tcatatttaa attgatctgc ttttaatgaa
    29881 gcaccaaaat taccattgtt accgatttca gtattaatag tatattcttt attgttagtt
    29941 gctttcggtg ctaaatctct atttttgaca tataaatgtg atcctgagaa tagacctaca
    30001 ttatcaatga atgaatagta tttataacca tctttatttg ttgtaattct tgaagcatca
    30061 gcaactgctg tattgtcagg aataaattgt aaatctaaac gatcagcatc agttggtact
    30121 ttaaataaat gtgcagtacc agtcttagca acatttacag ttgctaattc agtatttttc
    30181 ttagcatctt taataactaa atttgttgca ccttgcttac tattcgtttg catagtagta
    30241 aagttaataa ttaattctga atctggtttt acatttacag tttttgaaat accgttaaag
    30301 ttaccatgat ctgtagaatc atttgcattc acacgaccta atgcagccac gtttccttta
    30361 gcttgatagt tttgagggtt attcttatca aacatatcgc ttcgtcttaa ttctgagtta
    30421 acgaaaccaa tcttaccgtt gttaattaat gaataaccat ttactttatc tgtaacagtt
    30481 acagttggat cttgtctatt ctcatctgtt gatatggcag gatcatcaaa tgttaatgtc
    30541 gtattaatac tgccttcacc agtattgcta gcatttggat cttgagtttg tgcgtttgct
    30601 gctacaggtg ctgctggttg cgctgctgct ggagcattcg ctggctgtgt ttgatttgcc
    30661 ggtgttgcat tattattagg tgttgcttgg ttatttcctt gacctgcttg gtttgccggt
    30721 gttgcttgat ttccaggttg tgcttgtgca gcgttattcg gatcagcttg attaccttgt
    30781 ccagctggtt gtgtatttgg ttgtgctgct cctcctgctg gattagcctg tccaccttgg
    30841 tttgctggtt gtactgctgg ttgtccttgg ttggcaggtg cagctggctg tgctgtagga
    30901 ttagcttgag caccagcatt tgcgttaggc tgtgtattgg catcagctgg ttgtgctggt
    30961 tgattttgtg caggctgatt ttgctctgct gcagacgctg ttgtcgggtt agtagatata
    31021 aaagtaacag tggcaattaa agctgaaaaa ataccgacat taaattttct gatactaaat
    31081 ttttgttgtc tgaataaatt cattaagtca tcctcctggt tgattattct cgctgttaaa
    31141 tgatttcact taatcaactg ttaagataag tagtagcatc tgcgttaaaa acacaaagca
    31201 actctatcta attaaaatta attttatcat cattatatat tgagtaccag tgtattttat
    31261 attacatatt gattactttg tttttatttt gtttatatca ttttacgttt gtactataaa
    31321 ttatttctac aaacacaaaa aaccgatgca tacgcatcgg ctcatttgta atacagtatt
    31381 tatttatcta atcccatttt atcttgaacc acatcagcta tttgttgtgc aaatctttca
    31441 gcatcttcat cagttgctgc ttcaaccatg acacgaacta atggttctgt tccagaaggt
    31501 cttactaaaa ttcgaccttc tccattcatt tctacttcta ctttagtcat aacttcttta
    31561 acgtcaacat tttcttcaac acgatattta tctgttacgc gtacgttaat taatgattgt
    31621 ggatattttt tcatttgtcc agctaattca cttagtgatt taccagtcat ttttattaca
    31681 gaagctaatt gaataccagt taataaacca tcaccagttg tattgtaatc catcataacg
    31741 atatgtccag attgttctcc acctaagtta taattaccgc gacgcatttc ttctactaca
    31801 tatctgtcgc caactttagt tttattagat ttaattcctt cttgttcaag cgctttgtaa
    31861 aaacctaaat tactcataac agtagaaaca atcatgtcat tattcaattc ttgattttta
    31921 tgcatttctt gaccaataat aaacataatt tggtcaccgt caacgatttg accattctca
    31981 tctactgcta tgattctgtc tccatcgccg tcaaatgcta acccaaaatc actttcagtt
    32041 tcaactactt tttcagctaa tttttcagga tgtgtagagc cacatttctc attgatatta
    32101 tatccatcag gactacatcc aattgtttca gtatctgctt ctaagtcacc aaataagaat
    32161 ggcgctagtg atgatgttga accatttgca ccatctaaag caattttcaa accttcaaag
    32221 ttaacatcta ctgttgattt taaatagctc aaatattttt gtgccccttc aaagtaatct
    32281 gaataatgta caatatcatt gccaactggt cttggtaatt ctgggttttc ttgatccaat
    32341 aatgcttcaa tttcattttc ttgttcatct gatagtttaa aaccatctga tccaaagaat
    32401 ttaataccat tatctgcaac tggattatga gaggctgaaa tcattacacc taactctgca
    32461 cccatatcgc gtgttaaata tgcaacacct ggtgttgaaa taatacctaa tcgcatcact
    32521 tctgcaccaa ttgaaatcaa accagctatt aatgctgatt ctaacatttc acctgaaact
    32581 ctagtatcgc gacctacaag tacacgtggg tgtttttcac ctttattatg tgctagaaca
    32641 tagccaccgt atcttcctaa tttaaatgcc aattcaggtg ttagttcttg gtttgcgaca
    32701 cctcttactc cgtctgtacc aaaatatttt cccattgttt actctccttt aatttagcta
    32761 tttattttac atttatataa gccttcgttt cacttggttg tgctttagtg acatgttctg
    32821 gtaaattgat ttttacagtc ttttcagttg attctgaaat accatctaaa tctacttctg
    32881 catcaacttc gcttatattt tgtaagtcat ctcgactacc gaagatttca atttctttat
    32941 cttctaaatc aatcgaactt aactctttat catctgctaa actacctttc tgtttaacat
    33001 ttactttaac ctttttgcta aaaggctcta ctttaacttg taaattcact tcattaggtt
    33061 gaatcgatac atttaattta ttcagttttt tatcaaaagc cgttacttct gcgacatctt
    33121 ttgtgtcacc attaatcttt ttattagttt taaaagtggc ttttaaataa gcgatatcat
    33181 tcaattgttc ttctccacct gttactttaa ctgtttgtgg tgaaacttct tgctttgtaa
    33241 ttttataaag tggatcaata tcactctgac ttacatcagg ttgaactgtc atctttttag
    33301 ttactttgtt ttcaagcgta atatttgcta atttaggttt tacagaataa tgaatgtcat
    33361 cagctaaccc tttaacttga tacttctctt gatattttcc agctttagca tttgataaat
    33421 caatcactac tcttaaatct tctggatttt caatttttat tatctttgat tgtggtcctg
    33481 aaatagtcac attaactgtt tcaggcgctt ttgttaaatg caaatcttta gtgttataaa
    33541 gaatttcaac gggtacatct tgaatcgttt tactagactt ttgaccaaga ttaccagtgt
    33601 taaagatatt tccaaaaaca ttgttaacag ataaaaagaa aaacaatgcc aaaagaaagg
    33661 caataaatct caagccccat ttactttcta gcatattatt tcacaccttt cttttgaaag
    33721 cgtgtgccaa accaatgttc agcaagcaac tcttcaaaaa tttcgtttga aatgtctcgt
    33781 cgtaattttc catcaaatgt taccgaaata tcaccagttt cttcagatac aataacggta
    33841 aatgcatcag atacttctga aataccaacc gcagctctat gtcttgtacc caaactttta
    33901 gatatcttag gactatcaga caatggcaaa taacttgctg ctgctgcaat cttcgtgcct
    33961 tgaataatca ttgcaccatc atgtaaaggt gtgttaggta taaagacatt aattaaaagt
    34021 tcttgcgaaa tatttgaatc cattgcaata cctgtttcaa tataatcttg aagacctgtt
    34081 tctttttcaa agacaattaa tgcacctata cgtcttttag ccatatattg cacagccttt
    34141 gaaaccgatt gaatcaattt ctcttcatct ttactatacg tattagaagt atagcgtttt
    34201 aaaaagctac ctctaccaag ttgttctaac gcacgtctaa tttctggttg gaatattact
    34261 attaaagcta atacccccca ttgaataacg atatcgaata atttagatgt tgcagtcaag
    34321 ttcaatatca tacttatctg ctgaccaata acaattacta atatcccttt aagtaattgt
    34381 atcgctttag ttcccttaaa gaccgtgatg agaagataaa gtacatacca aactatcagt
    34441 aaatcaagga tactcgttac aatttttaac gtactgaggt tttgaaaaaa gttggaaaaa
    34501 tccataacat ctcctccggg taatattttt ccataatacc cattatacca atcatttaca
    34561 tcactgtcat acgaacatat atttaaatag aaaaaattta ttttaaagat tataactact
    34621 cttaatcatt ttagtgaatt aaaaaaagta gtgcaaaaag caaaatatac tttatacact
    34681 acaaatcatt tatttataat aaagtttcac caaaaaatgt tccaactaat gaaaccgctt
    34741 gttcagcagt atgattatta ctgtcaatca atggatttac ttcaactaaa tccattgagg
    34801 aaattaaatg tgattgatgc agtaattcca atgcaaaatg gctttctcta taactaagac
    34861 cacccaaaac tctagtacca gtgcctggcg tttcaagcgg atctaaagca tcaacatcta
    34921 aagataaatg aacgccatca acattgcgtg acttcaaata ttctattgta ttttcaatta
    34981 cttcctttat ccccaattta tcaatatctg acatagtaaa tgttttaata ttatgatctt
    35041 tgataaattg tctttcacct ttatctaaat ctctcatacc aattagtacg atgttttctg
    35101 gcttgattac attactattt aattctaaaa gttctttggg accttcgcct gtcaaaatcc
    35161 ttagaggcat accatgaata tttccacttg gtgactcttc aggtatattt aaatcaccat
    35221 gtgcatcata ccaaataaca cctaaattat tataatgttt acttattgct gatactgaac
    35281 ctaccgcaat agaatgatct ccaccaagaa ctagaggaaa tctgttattt tcaattgatg
    35341 ctgaaacctc tttatttaat ttttgattaa catctataat ttcatcataa tttcttaatc
    35401 ctttttgttc actatgaaat ttttcaatgt tcacagcagg taccttaata tcccccttgt
    35461 catatacatc aaggtctaat tgctttaatc ttgaaattaa tccagcatat ctaattgctg
    35521 ttggtcctaa atcaacacct aattttcttt gtccaaatgt tgatggtgca cctataatat
    35581 caattgcttt tgtctttgtc ataagcgtcc ccctttgctc tatgtaatta aagaataatg
    35641 tatgcgctta ccattatcaa gcaatagcta cacatataat ctgtttatct tattacttca
    35701 taaaaaaagg ttcttcatct tttatggtgg gaaggtaaaa cttcctgctt tttttaatac
    35761 acaaaaagcg caattgcctc tataatttaa agtgaccaaa cccaaactaa aggagacaag
    35821 tgcgcctatg tgtaatgata ccttagaatt actaagaata aaagatgaaa atataaaata
    35881 tataaaccaa gaaattgacg tcattatcaa aggaaaaaaa gcaacagtgg ttaatgctgt
    35941 actaacgtat aagccttcgg cctgttattg ttgtggagtt aaaaatgaag gacaaattca
    36001 taaacatggt aagcgtgttt ctcgtattac tttacttaaa actcaagggt ataacacata
    36061 cctcaactta gctaaacaac gttttaaatg cttagaatgc aatggcactt ttactgctaa
    36121 aacgtcaatt gttgatgagt cgtgttttat ctcaagatgt gttactcaaa aagttataga
    36181 agaagctact aaagttaaaa cagagattga tactgcagaa gataactgta tctctccatc
    36241 tactgtaagt cgtattagaa ctaaagcggc taattcatta cgaattaaac cctttaattg
    36301 tttgccagaa cacatcgcta tggatgaatt taaaagcgtt aaaaatgtaa ctggatcaat
    36361 gagtttcatt tttatagata atgatactca tgatgttata gatattttag aaaatagaac
    36421 tacaagattc ttgcgtgcct atttcgagcg attcgattta aaaaatcgac aacaagttaa
    36481 gacggttact attgacatgt atgaacccta tgtccgatta tttcgcgacc tatttcctaa
    36541 tgcagctatt atttttgaca gattccatat cgttcaacat ttaaatagag aacttaataa
    36601 gtatcgtgta caagttatga atgaataccg taataaaaaa ggacctgatt atacaatttt
    36661 taagaataac tggaaagtcc tattgatgga tactagtaaa accatattta gtaaatacag
    36721 atggaataaa tcttttaagg cttataaacg ctcatctgac attgtagaat tcatgctttc
    36781 aaaagacgat atactacgac actcctacga acttgtccaa ggattacgaa aagacctaag
    36841 gttatgtaat tggcctaaat ttattaatcg tttaaattca gttagtaaaa agtctg
//
LOCUS       DALAAR010000023        36140 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000023 DALAAR010000000
VERSION     DALAAR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.023"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            104..550
                     /locus_tag="O2458_001140"
     CDS             104..550
                     /locus_tag="O2458_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3887011.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            766..1194
                     /locus_tag="O2458_001141"
     CDS             766..1194
                     /locus_tag="O2458_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCZ3887012.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1301..2470)
                     /locus_tag="O2458_001142"
     CDS             complement(1301..2470)
                     /locus_tag="O2458_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCZ3887013.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2840..3478
                     /locus_tag="O2458_001143"
     CDS             2840..3478
                     /locus_tag="O2458_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCZ3887014.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            3497..3754
                     /locus_tag="O2458_001144"
     CDS             3497..3754
                     /locus_tag="O2458_001144"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887015.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3733..4473
                     /locus_tag="O2458_001145"
     CDS             3733..4473
                     /locus_tag="O2458_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ3887016.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4731..5384)
                     /gene="nreC"
                     /locus_tag="O2458_001146"
     CDS             complement(4731..5384)
                     /gene="nreC"
                     /locus_tag="O2458_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCZ3887017.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5407..6441)
                     /gene="nreB"
                     /locus_tag="O2458_001147"
     CDS             complement(5407..6441)
                     /gene="nreB"
                     /locus_tag="O2458_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCZ3887018.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6465..6917)
                     /gene="nreA"
                     /locus_tag="O2458_001148"
     CDS             complement(6465..6917)
                     /gene="nreA"
                     /locus_tag="O2458_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCZ3887019.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6937..7614)
                     /gene="narI"
                     /locus_tag="O2458_001149"
     CDS             complement(6937..7614)
                     /gene="narI"
                     /locus_tag="O2458_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCZ3887020.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7607..8182)
                     /gene="narJ"
                     /locus_tag="O2458_001150"
     CDS             complement(7607..8182)
                     /gene="narJ"
                     /locus_tag="O2458_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCZ3887021.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8175..9734)
                     /gene="narH"
                     /locus_tag="O2458_001151"
     CDS             complement(8175..9734)
                     /gene="narH"
                     /locus_tag="O2458_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCZ3887022.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9724..13413)
                     /locus_tag="O2458_001152"
     CDS             complement(9724..13413)
                     /locus_tag="O2458_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCZ3887023.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13722..14699)
                     /gene="cobA"
                     /locus_tag="O2458_001153"
     CDS             complement(13722..14699)
                     /gene="cobA"
                     /locus_tag="O2458_001153"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCZ3887024.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14690..15004)
                     /gene="nirD"
                     /locus_tag="O2458_001154"
     CDS             complement(14690..15004)
                     /gene="nirD"
                     /locus_tag="O2458_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCZ3887025.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15008..17413)
                     /locus_tag="O2458_001155"
     CDS             complement(15008..17413)
                     /locus_tag="O2458_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3887026.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(17479..18210)
                     /locus_tag="O2458_001156"
     CDS             complement(17479..18210)
                     /locus_tag="O2458_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCZ3887027.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18440..18976)
                     /locus_tag="O2458_001157"
     CDS             complement(18440..18976)
                     /locus_tag="O2458_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887028.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19209..20033)
                     /locus_tag="O2458_001158"
     CDS             complement(19209..20033)
                     /locus_tag="O2458_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ3887029.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20250..20432)
                     /locus_tag="O2458_001159"
     CDS             complement(20250..20432)
                     /locus_tag="O2458_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887030.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20516..20983)
                     /locus_tag="O2458_001160"
     CDS             complement(20516..20983)
                     /locus_tag="O2458_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ3887031.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21169..22719)
                     /gene="adcA"
                     /locus_tag="O2458_001161"
     CDS             complement(21169..22719)
                     /gene="adcA"
                     /locus_tag="O2458_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCZ3887032.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(22908..22979)
                     /locus_tag="O2458_001162"
                     /pseudo
     CDS             complement(22908..22979)
                     /locus_tag="O2458_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23014..23280)
                     /locus_tag="O2458_001163"
     CDS             complement(23014..23280)
                     /locus_tag="O2458_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCZ3887033.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23280..23531)
                     /locus_tag="O2458_001164"
     CDS             complement(23280..23531)
                     /locus_tag="O2458_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ3887034.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(23696..23797)
                     /locus_tag="O2458_001165"
     CDS             complement(23696..23797)
                     /locus_tag="O2458_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887035.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(23804..24403)
                     /locus_tag="O2458_001166"
     CDS             complement(23804..24403)
                     /locus_tag="O2458_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCZ3887036.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24422..24781)
                     /locus_tag="O2458_001167"
     CDS             complement(24422..24781)
                     /locus_tag="O2458_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ3887037.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            25036..26286
                     /gene="fmhA"
                     /locus_tag="O2458_001168"
     CDS             25036..26286
                     /gene="fmhA"
                     /locus_tag="O2458_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCZ3887038.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26383..27114)
                     /locus_tag="O2458_001169"
     CDS             complement(26383..27114)
                     /locus_tag="O2458_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887039.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(27111..27830)
                     /locus_tag="O2458_001170"
     CDS             complement(27111..27830)
                     /locus_tag="O2458_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCZ3887040.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27811..28590)
                     /locus_tag="O2458_001171"
     CDS             complement(27811..28590)
                     /locus_tag="O2458_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCZ3887041.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28711..30150)
                     /gene="mdeA"
                     /locus_tag="O2458_001172"
     CDS             complement(28711..30150)
                     /gene="mdeA"
                     /locus_tag="O2458_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCZ3887042.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            30324..30428
                     /locus_tag="O2458_001173"
     CDS             30324..30428
                     /locus_tag="O2458_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCZ3887043.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30582..31268)
                     /locus_tag="O2458_001174"
     CDS             complement(30582..31268)
                     /locus_tag="O2458_001174"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCZ3887044.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(31595..32461)
                     /locus_tag="O2458_001175"
     CDS             complement(31595..32461)
                     /locus_tag="O2458_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ3887045.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(33014..33118)
                     /locus_tag="O2458_001176"
     CDS             complement(33014..33118)
                     /locus_tag="O2458_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887046.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            33213..34523
                     /gene="sbi"
                     /locus_tag="O2458_001177"
     CDS             33213..34523
                     /gene="sbi"
                     /locus_tag="O2458_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCZ3887047.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            35059..35988
                     /gene="hlgA"
                     /locus_tag="O2458_001178"
     CDS             35059..35988
                     /gene="hlgA"
                     /locus_tag="O2458_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCZ3887048.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT        11791 a         6893 c         5105 g        12351 t
ORIGIN      
        1 aacatttaaa ttcaattata gacaattcaa taattgcgat gaaatcagta cttgacaact
       61 attacacttc ataatattgt ttattttaac aaggagtgct tggatgtatg tagaaaacag
      121 ctatcttagc aaacagttgt gttttttatt ttatgtttct tcaaaagaaa taattaaaaa
      181 atacacaaac tatcttaagg aatatgattt aacctatact ggttacattg ttttaatggc
      241 gattgaaaat gatgaaaaac ttaacatcaa aaaattaggt gaacgtgtgt tcttagattc
      301 tggaacactg acaccattac taaagaaatt agaaaagaaa gattacgttg ttcgaacacg
      361 tgaagagaaa gatgaaagaa acctacaaat ttctctaaca gaacaaggta aagcaataaa
      421 aagccctctt gctgaaattt ctgttaaggt ctttaatgaa ttcaacattt cagaacgaga
      481 agcatcagat ataattaata atttgcggaa ttttgtttct aaaaattttg actattccga
      541 caaaaaataa ctccctatca taaggtgatg ggcagaagta tgcatcgata tgtttattca
      601 ttatctttat tgcttgtata aatctcgatg ctcagttctt ttgccctgat tccttatcag
      661 gcacaaatct acccatttat tactttttta tctccccttt tactttaact tttttaatac
      721 tactcatata atggctttgt aatacaaatt tctataatgg aagtgatgat attgaacttc
      781 aatcaattcg agaatcaaaa cttttttaac gctaatccaa gtgatacatt taaagattta
      841 ggtaaacaag tatttaatta cttttcaaca ccttcatttg taacgaatat atatgaaaca
      901 gacgaattat attacttaga agctgaacta gcaggtgtaa ataaagaaga tattagtatc
      961 gatttcaata ataatacgct cactattcaa gctactagaa gcgcaaaata caaatctgaa
     1021 caactcattt tagatgagcg taacttcgaa tcattaatgc gtcaatttga ttttgaagct
     1081 gttgataagc aacatattac tgctagtttt gaaaatgggt tattaaccat taccttgcct
     1141 aaaatcaaac caagcaatga aactacttca tcaacatcta ttccaatttc atagttaaat
     1201 ttaaaatgaa tacactacta aagccttaat gtcatgctac gaatattgcg catgatatta
     1261 aggctttgta tttaactaaa tgaatggcat gtattacata tcaaccgttt ttcaatgagc
     1321 catattcttt ttggtataaa tgacgcatgg taaataatgc aatacatcca aataccgcta
     1381 agaaaataaa tgctaaatga cttgatcctg ttaaattagc tacgtacgtg attactagtg
     1441 gtgggaagaa tcctcctaaa ccacccatca ttgatacgat accatttgcg gatcccgctt
     1501 catttaagaa gtatgatggt actaatttga agattaagcc gttaccaata cctgcacaaa
     1561 tacttattgt taaacagcct acagtgaata atgcgatatg gtctgaaata cctaaaataa
     1621 tggcaccgat aatcataaca acaaaatcaa tcatcaatac tttaactgca ttaaatttat
     1681 cacctaaaat gccacctatt ggtcttaaga atgttgccag cgcaatgaat acacctgatc
     1741 gaataccagc atctactttg tcaattccaa aatgatttac taagtagtta ggtaagaaaa
     1801 taccaaatgc tacaaaagca ccaaaagtaa taaaatacca ataacttaag taatataatt
     1861 tgtagttttt agataatgtt ttcatttgag ccattaatgg tactttaatt ttacgttctt
     1921 gtgtgtcacc aaaaatgaac ataattaatg caaataaagc gataataatt aagtaacttc
     1981 taactgttgt ttgccaacca ataatacccg ctatcggtgg tgctaaaaat gaagaaactg
     2041 ctgtaccgat atttcccata ccataaatac catttgctag acctactttt tctttaggga
     2101 aatatttagg aactgatgta acaccaactg agaaaattgc accacctaca ccaaggaaaa
     2161 atcctgaagc cattaacata cccggtgttt gtgcttggct taagaaaaat atcgggaata
     2221 acaatacgat aaaactagta aagaataccc atttagcgcc aacaatgttt gttaaataac
     2281 caaatggcac acggagcacc gatcccaaaa taactggtat cgctaaaatg attgatattt
     2341 gaccttcagt aacattgaca tcttgtttaa taaagggcat taatggcgca attatactcc
     2401 aagccataaa cccaaccact aaacttaatg tttgtaatgt aagttgaaag ccaccttttg
     2461 ttttgtacat acgtttcacc ttcatcaaaa tcatttattt tttaatagat atcaatactc
     2521 tgcctatata ttagaggtca atgttattta aaaatgcttt catttatatg aaaacattca
     2581 atatagacaa agtattgact acatatatat tttattatga ttgtgaaaaa gtgaacataa
     2641 ggattttccc ttatttatga agtgatttac cctatacaat gtccctttaa gatgctttta
     2701 gacacttatt cgccatcatt ttaatcatat tatttaggaa cactcacata cttttcttaa
     2761 tttttttcaa ttagttacaa ttactgcttg aaaatcacgt tacgttatcc tttatgctga
     2821 caacataagg aggtttatca tgtataaaat caatattatt agatcagatt ccgtctataa
     2881 taatatactc aaggcaccaa taaacgaccg tgattcaatt ttcactaaag aaatattagt
     2941 accttttaaa aagaaatttg aggtacaaca tatgcctatt tataatgatg acaaacagac
     3001 aatgagtgcg attcaatttt tagatgcctt tcaaatttct cccaaagatt tacgcatgtc
     3061 tgaccaaatg tcaatacaat acctgaataa tgatttctgg agtaattgcg aaaaatattt
     3121 aaaagttgct atagatcaat tttcaaatta tagtatctca tctcaagttt ctaactatca
     3181 tttcacagta ttacttggag atcgccaaaa accacttatg tatctaaata aaaatcgcgg
     3241 tggtgatggt ggcataccag gttatattat gatttattta gtgccgagta caagtacaat
     3301 taattctatg aaaagcttaa ttgcacatga agtaaatcat aatatgcgct atcaatatat
     3361 tgattgggat ggcggaagtt tgattgaatt gattattgca gaaggattag ctgaaaacta
     3421 tgtagaatca ttgtatggta aagcgcatat tggaccgtgg gtaacaaata ctaattgaag
     3481 tcgtgataac gtaaaaatta aaaatactat ttataatcat ttacatttaa agcatatatt
     3541 tgaatcgatg ccttatctct atggtgatga tattaataaa cttcaaggta ggcctatcgt
     3601 tggcttatct catgctgccg ggtatgcatg tggctatcac ttggtaaaat actttttaca
     3661 aaaaacaaac atacctattg aagttgctac aacacttcca gcacaaaaaa taataaatga
     3721 ggtgactgaa ttttggcata cacatacact ttaaaagata ttattgaaat tacaggtgta
     3781 actaaaagaa ctttacatta ttacgatgaa ataggattat tagttccaga taaaaatgat
     3841 aaaaattatc gcgtttataa acagcaagac ttagaaaaat tacaaaagat tttaatactc
     3901 aagtcttttg attttgatat cgctaaaata aaacaataca tttcgtatga taatgaacaa
     3961 ttgcgaaagt tattatcaga gcaaataagc aagttagata aaaagatttc tgacttacaa
     4021 ttaattagac gctctgtatg tgaatttatt aaaggactct ctctaataga taccagcatt
     4081 ttaaacaaga cactacagtc acaatatgat aaagaagcat ctataaaata tggtcatacg
     4141 aaagcatatc aatcatttat tagacgtaaa gacagcttac aatcgcagga tatcagacat
     4201 aaattgacaa ctatcttcaa taaatttaat catatgtctt tgagtcatta tccaatccaa
     4261 gattgtagtg atctcgtatt tgagtggaag gcatttatga acactatcgc tgattttgat
     4321 gatgaaacat tatgctgtat tgctaaaaca tatgaagatg atacgcgttt caaagattac
     4381 tttaattcat atgataatca aaatttagca tcatacattt cagaagctgt taattatttt
     4441 ttgagcaatg tgaataagag cgacaatttt taatcctcat acagatgcta caacactgaa
     4501 tacaccaact aaaataattg ttcaaagtag aaaactaatc atttattctc taaatcaata
     4561 actatattaa aagttatacc tttgcaaagc gaattagtat aggttaccga aaggagaaag
     4621 gattaggttc cattcgattt ataaaaaaat atatttcgaa acatacaaaa gcgccagaag
     4681 ataatcattt cctctcgcgc ttttatactg aattatatta atttcaaact ctaaaactct
     4741 aataatttct ttttcaatgc atattcaact agctcaggtt tactctttaa gccaagcttt
     4801 gtcataatat gcgtcttatg tgcttctact gttttcacag atacaaataa tttctctgca
     4861 atttctttat tcccgtaacc tttggcaata agtggcaata tttctagttc tcgttttgat
     4921 aagattttaa aaggatctgt tgtgtttgca gtgtcctgat ttgaattaga tacaaattca
     4981 ttcactaaag atgttgtaag tttcatatct acatatgttt cacctttata tacagttcga
     5041 atagctaaca ataattgttc atcaggtgca tttttcaata tgtaaccttt cgcaccatta
     5101 cgcaacacat ggaacaaata ctcctcatca tcaaacattg ttaatattag tattttagtt
     5161 tcaggaaaac tgtcagcaat tttactcgta gcgataagac ctgactcacc tggtggcata
     5221 cttaaatcca ttagtaacac atcaggttta tattccatta ctttttggta agcttcgacg
     5281 ccatctgcag ccgttgcaac aacttccata tcattttgat aatttaaaat catagagaac
     5341 cccgtacgga caacagcgtg atcatcggca atgactattt tcaattttat tcccccaatg
     5401 tatgtttcaa attggaatgt tcaatgtaac attggtaccc tcaccaattt tcgtttcaat
     5461 attgacgcta ccgctgacta actcagctcg ctcattcatt ccatataaac cgagtccaga
     5521 acctttaggc ttagaacttg gatcaaaacc atttcccgca tctatcactt ctgctaccaa
     5581 atggcgccca gtttgacgga tacctacatt tatttcattt acatcagcgt atttcaacgc
     5641 atttaaaata gcttcttgca ctactcgata aacaaccgtt tcaatatcac tatcaaagcg
     5701 agtattttta atatttgatg tatatatgat ttttattcca taattttctt caaactgttt
     5761 aaaatatgat ttaaaagctg cttcaaggcc tagatcatcc aaagaagcgg gtcttaattc
     5821 aaccgacata ttacgtatat catcaattaa tttagcgaca atatattcaa tattttctgc
     5881 gtcttccaaa agcttagttg tatcttcttg atattttaat aatctcaatt gaacatctac
     5941 attgagcatt tcttgaatca cactatcatg taactctcta gaaattcgct ttctttcatt
     6001 ttcttgggct gagattgttt tacgcatcat acgttgttga tgcaatttct cttgctgttc
     6061 aatttgtgat gaaacatttt gaagcgtaaa tgcatgaatt cccctgtctt gatcaatcaa
     6121 ctgatatgtt gctgtaaatg gcatcacttt ttgatctttc gtcttcataa atacttggaa
     6181 attcgtagct tgtacttgca tcgattctaa gaaacagtct ttacatgatt gtacatcata
     6241 ggcatttgta tacccttcac atctgtgaca aatcgcattc gccacagcac tgtaattatc
     6301 ttcttcagat aatatatctt tagcagcatc attcattgca ataattttac cgttatcatc
     6361 agcaaaaact atcttttcga ttgaatgctc ataatatttt ttcaataaag tatctaactg
     6421 tatactgtcc tcattaatca tgacttacac cctaattcat ctcattattt atcatcattg
     6481 aaaataccaa acttacgttg aatatcatca ttatcaaata tttttggtaa aggacgacca
     6541 tctctttgac caaataatag tacgccatac acttgattct tataccaaag cggcactgct
     6601 aaaactgctg ttaatgattc gctcaataaa attggatagt caatcttttc ttcaggccct
     6661 aaagctaaac caacattggc tattaccata cgctttcctg ttttcataac agttccagct
     6721 aatccacgac cttttcttaa aataatcaat ttaaatcgat tatttttatt acctgaaaca
     6781 tagtgccatt ttattggaga tgatggtttg ttagattcat agaaagcgat tgccgcaaaa
     6841 tcataaccct cttctttgcg tattttatct aatgtctctt gaaatctacg atcttcaatt
     6901 attgcttctg gtgtcaaatc ctttcacctc ttatgcttac actttattct tacggtaaat
     6961 aatatatctg cgatttatat atgtcaaagg tacactccaa acatgcacca aacgtgtaaa
     7021 tggccaacaa gccataatag tgaaacctaa caatatatgc attttaaatg caatcggcac
     7081 accactcatc aatgacgcat ctggttttaa cataaataat tgtctaaacc aaattgataa
     7141 tgaagttctg tagttaaagt ctggatgttg tatatttgtt actaatgttg cgtaacatcc
     7201 cataaatacg ataagtaata ataagaaatt tacaaatata tccgacgctg aacttaatct
     7261 tcgaatactt ttcgtagtaa cacgtctcgc tgttaataaa aacatcccta tcaaagttat
     7321 tataccaaag atgctaccaa tataaacagc gcctatatga tataaatgct cagacacacc
     7381 cactgcatcc atccatggtt tcggtattaa caatccaact acgtgtccaa aaaacactgg
     7441 aataatacct aagtgaaata ataaacttcc ccacatcaac ctttttcttt ctattaattc
     7501 actagattta gctgtccaag aaaatttatc ataacgataa cgtgcaatat gacctgcgac
     7561 aaagacaact aaacataaat acggaaatat aacccataaa aactgattaa gcatgatgtt
     7621 tcactccttt tggtgatgtc aaacataatt tcaatgtttt tctaagtgct tgaatcacat
     7681 aggcatatgg attgttatct tcaccaagtg cattcgccat cacatatgtt ccatcctcaa
     7741 taatcataat gattaattga atattctctt cagctcttgg atcatttcgc cattctgcca
     7801 cttgcaaaaa ttgaagcatc aacggtagat aatcagaaag ttcattatct accatttcta
     7861 gtccaaacat ttcatataat acctttaatt tagctaacat ttgcccacgt tctttttgcg
     7921 tatcaaattt gttatacgtc atatataatg gtgctttttt cgtaaaatca aatgtatctg
     7981 tataaatcgc tttgatttct gataatgaaa atgtcatcat tacatttcta aatgctacta
     8041 aatcttcata tgctggatga tctttcgaaa ttgtttcttc aaaagttttt ggatgaaaag
     8101 ttaatttttc tggaaaacat aactgttgtg ccatatatcc aaaactttct tgatattttt
     8161 taaaattatc gaaattaatc acggaaaatc cctccataga aattctcatt ataaatttct
     8221 tgaccagttt tccctgaacc tactgcaacg ccacagcctt cacagttatc tccaaaatgc
     8281 tcgccgccgt aattgtatcc tgtactacct tgtgcgtgat acgtatctaa ataggtttct
     8341 ttgtgtgatg ttggaataac aaatcgatct tcatatttgg ctagtcctaa taaacgatac
     8401 atgtctttag tttggcgctc ggttatacct aatcgctcta atcgagacgt gtcaaatggc
     8461 tgttgagtaa cttgagatct catataactt ctcatcattg ccatacgttg tagggctcct
     8521 tttactggct ctgtatctcc tgcagtgaaa atattagcta agtattcaat aggtaaacgc
     8581 atttcttcaa tggctgggaa aatcgcatct ggattttgag ttgtattttt accttcaaaa
     8641 tagctcataa ttgggctaag tggtgggcaa taccaaacca tcggcatcgt tctaaattca
     8701 ggatgtaacg gaaatgcaag tttatattca attgctaact tataaattgg agagttttgt
     8761 gcagcttcaa tccaatcgta accaatacca tctttttcag cttgagcaat gacttcttcg
     8821 tcaaatgggt ttaagaatat atctaattgt ttttcatata aatctttctc gtctactgct
     8881 gaagctgctt catgaactcg atctgcatca tataataaaa cacctaagta acgcatacgt
     8941 cctgtacaag tttcagagca taccgtaggc atacccgcct cgattctcgg gaaacagaaa
     9001 gtacactttt cagctttgtt cgttttccaa ttgaagtaaa ctttcttata tggacaacct
     9061 gtcatacagt aacgccatcc acgacatgcg tcttggtcaa ctaatacaat gccatcttca
     9121 tcacgtttat acatagcacc tgaaggacac gatgcaacgc aacttggatt caagcaatgt
     9181 tcacataaac gtggtaaata catcataaaa gtttcgtcaa attggaattt aatatcttct
     9241 tctatttttt ggatgttagg atcttttgga cctgtaacat gaccacctgc taagtcatct
     9301 tcccagttag gtccccattc aatttcaatg ttatcccccg taatttctga atacgctcta
     9361 gcaactggcg aatgcttccc tgatttcgca gttgttaaat gttcataatt atagttccat
     9421 ggctcataat aatctttaat taatggcata tctgggttat aaaaaatttt acctaaagca
     9481 atttttgaaa ttctacttcc agattttaat tcaagtttcc ctttacgatt tagtacccaa
     9541 ccacctttgt agtgttcttg gtcttcccaa cgtttcggat accctacacc tggcttcgtt
     9601 tctacgttgt tgaaccacat gtactcagca cctggacgat ttgtccaagt gtttttacat
     9661 gtcacactac acgtatggca tcctatgcat ttatctaaat ttaataccat cgcaacttgc
     9721 gctttaatct tcaagccaat taacctcctt catctttcta actgctacat ataaatccct
     9781 ttggttccca attggtccat aataattaaa gtgataacta atttgtgcgt atcctccgac
     9841 tagttgtgtt ggtttcaaat ggattctagt cggcgcgttg tgtgaaccac cacgtgtatc
     9901 tgtaatttct gacccaggcg tttgaatatg tttatcttgt gcatgataca taaacattgt
     9961 acctttaggc atacgatgcg aaataactgc tcttgccgtt acaacaccat tacggttata
    10021 cacttctagc caatcattat cttggatatc gtgtttttca gcatcttcat ttgatatcca
    10081 aaccgttgga ccacctctaa atagtgtcaa catatgctta ttatcttgat acattgagtg
    10141 tatattccat tttccatgag gcgttaaata acgcagtacc aaagcatctg taccaccttt
    10201 aattttctta tctctattcc caaataccat tggcggcaat gtcggtttat atactggtaa
    10261 gctctcccca aattgttgga aaacttcgtg atccacataa taactttgac gtcctgttaa
    10321 tgttctaaaa ggtactagac gttctatatt cgttgtaaat ggtgaatatc gtcgaccttg
    10381 tttatttgaa cctgggaata ctgctgtcgg tattacttct cgtggttgtg aagttatatt
    10441 taaaaacgaa attttctcag cagcgcgttc gctagaaata tcttttaacg gcattccagt
    10501 ttgttcttcg agatcttcat atgatttttg tgataattta ccattcgtag cagatgaaat
    10561 acttagtatt gcatcagcta cattacgtgc tgtatcaata cgtggacgat tcgctctcac
    10621 agaatcatca tttgtatcac tccacgtacc taacatactt tttaattctt catattgttc
    10681 actgacaccg aaacttacac catgtgctcc aactttccct ttttcaagta caggaccaag
    10741 cgtgacatat ttgtcgtaaa ttttagtgta gtcgcgttct acaattgcaa agttaggcat
    10801 tgtacgtcca ggtaccgctt caatttcacc cttcgaccaa tctttcacta cgccgtatgg
    10861 tgttgaaatt tcttgctttg tatcatgact aagtggagtt gtcacaacat ctttaaacgt
    10921 tccaggtaaa tagtcttttg ccatttctga aaatgctttt gccaacgttt tataaatatc
    10981 ccagtctgaa cgcgattccc ataacggatc aatggcagga ttgaaaggat gtacatatgg
    11041 atgcatatcc gttgatgata aatcatgttt ttcataccaa gtcgctgccg gcaaaacaat
    11101 gtcagaatat aacggtgttg ccgtcattct gaagtctaaa gagaccacta aatctaactt
    11161 acctgttgtt tcttcacgcc acgtaatttc ttctggcttt tcatcttcat ttggtgtagc
    11221 taataaccct gattttgtgc caagtaaatg cttcataaag tattcttgac cttttgcaga
    11281 acttgaaatt aagtttgaac gccatataaa taatgatttt ggatgattct ttttcaaatc
    11341 aggatcttct attgcaaatt gtgtttgttt tgatttcact tcatcaattg cacgttgcaa
    11401 aatcgcttca tttgaatcta taccttcatc tttagcttct tctgcaaaca acaaactatt
    11461 tttattaaat tgtggatatg atggtaacca accaagtcta gctgctaaaa cattataatc
    11521 agctggatgt tgatgcttta actcctctgt tttagctaat ggagatttta aacgatctac
    11581 atttgactct tcatatttcc attggtctgt tgcaaaatag aaccaacttg ttccgttttg
    11641 caaacgtggt ggtccttgcc agtctttcgc aaatgcgaca gtactccatc cttcaatcgg
    11701 acgacatttt tcttgtccca catagtgagc ccaaccgcca ccgttcacac cttgacagcc
    11761 acataacata actaagttta agattgaacg ataaatcgta tctgagttaa accaatggtt
    11821 aatacccgca cccatgataa tcattgaacg cccttcagta tcgatagcgt tttgcgcaaa
    11881 ttctttcgct acttgaatga caacactttg ttttacgcct gaaatggctt cttgccaagc
    11941 aggtgtatat tttgattctg catcgtcgta tccttttgat tctaatttat gatcaaaacg
    12001 acgcacgcca tattgacttg ccattaagtc aaaaattgta gcaatacgga ctttgtcacc
    12061 atttgctaaa gtgacttgtc gagttggaat tggacgattg aatatcccat ctccatcact
    12121 atcaaagtat gggaattgaa ttgtttctaa ttcgtatcca ccttctgtca ttgataatgt
    12181 agggttaatt ttagaaccat cttctgtttc tagttttaag ttccacttct taccttcttc
    12241 ccaacgttga cccattgtgc cattaggtac tactaaacta tcgctgattg catcatgaat
    12301 aactggcttc cattcgcctt gctctgttgt ttgacctaag tcactcgctc ttaaaaatcg
    12361 acccgcttta tatccatttt catcttcatc cagcatgata agaaacggca tatctgtata
    12421 ttgtttagcg taatttataa agcgttcatt aggttgatta acataatgtt cttgtaaaat
    12481 aacatgcgtc attgcttgtg caattgcagc atctgaacca ggattcggtg ctagccagtt
    12541 atctgcaaat ttcacatttt ctgcgtaatc tggtgctact gaaatgactt ttgtaccttt
    12601 atagcggact tcagtcataa aatgtgcatc cggagtacgt gttaaaggta cattagagcc
    12661 ccacataata atgtatgatg cgttatacca gtcacttgat tcaggcacat ctgtttgctc
    12721 tccccaaatt tgtggagagg caggtggtaa atctgcatac cagtcataaa aactaagcat
    12781 ttcaccacca agcaaattga tgaatcgagc acctgctgca taactaatca ttgacatcgc
    12841 tggaataggt gtaaatcctg cgattcgatc tggaccatat ttttttattg tatacagtaa
    12901 ttgtgctgcg attatctctg taacgtcttt ccaatttgaa cgcacgtgcc ctcccatacc
    12961 tcgggcttgc ttatattgtt tggctttgtc ttcattttca acaatagacg cccatgcagc
    13021 aacgcgatta ccattgtttt cttctaatgc ttcagtccat aaatcccaga gttttccacg
    13081 aatatatgga tatttgattc gaagcggact gtattcatac caagagaatg acgcacctcg
    13141 tggacatcct ctcggttcat attcaggcat atccggacca caacttggat agtcagtttg
    13201 ttgattttcc caggtaatca caccattttt cacaaatact ttccaagaac atgagcctgt
    13261 acagttaaca ccatgtgttg ttcttacttc tttatcgtgg ctccaacgtt ctctgtacat
    13321 tttttcccat tctctacttt tactttctag gatcgaccaa ttcccattaa atttttctgt
    13381 tggcttaaag aaattcaatc caaattttcc catatttata tcctcctacg tataaaaata
    13441 cgatgtgtag atgtcgtgtt tttaaatact ttaaaatgcc caagactatt gctttaatta
    13501 gattgtacat tttttcacaa acataaaata ttagggaatc acctaattac ttaaggaatt
    13561 tccctatcaa taacgggatt tcattgaaat aatacacaat catgtatggt catgcttatt
    13621 gccaatctaa atcgttcaaa tttggcacaa cgacaaataa ggcttcaaca cgaatatatt
    13681 ctctcggttg aaaccttact tattcattta ttttttataa attagtgaca taacactgta
    13741 ttagcatctg cacgatcggt tgaaatatat gttacatttt cttgctgctt aataaatgca
    13801 tcatagtaat catattgcga cgaatgatat gtgccattcg atgtatcatt tgggtttagc
    13861 aaacagccat aaccttcgtc atataaatgt tcacagagca taagggcgtc atgtttagaa
    13921 ccacttacta cataaaattg cttcatagga tcatatgatt taggagtgtt ttcagtataa
    13981 tcaacaactt cccctataat acatatacct ggtttcgcct caattgaata gtgttgcaat
    14041 tttgaaataa tattacttaa acgcccctta acaacaaact cgttaaaaca cgatgcttga
    14101 aagacaatcg ctatcgggta atcaatatct gtgtattgtt gtatctgtgt gataattttc
    14161 cctaaacgtt ttacccccat ataaattgct aacgtgccac cattcactaa ggaattgaca
    14221 tccacttcat tttcttctga atctttaaag tgacctgtag aaaatgtcac acttttagca
    14281 actgtacgca ttgtcaaacc tgtctgcata gtagcaactg ctgcgctcgc tgatgtcacc
    14341 cctggtacaa tttcaaacgc aatatgatgt tcatttagtg tgtcgacttc ttcttgcaca
    14401 cgaccaaata tcgctggatc gccaccttta agtctaacaa ccttgttata tcgacgcgct
    14461 gcttccacga tacagtcatt tattttttct tgctgaatat gttttgcata cggcttttta
    14521 ccaacatcga taatttcagt agtcaaattc gcatattgta aaattaacgg attcactaat
    14581 cgatcatata gaatgacatc cgcttcacgt attaaacgct cagccttttt cgtcaaataa
    14641 ttcggattac ctggacccgc acctatcaag taaaccttgc catattcctc tacagacata
    14701 tatatacgtt cccgtctgta acttctacct cataaacatc tacacaacct tcatcaggtt
    14761 cttgaacaat acctgtattt aaatcaattt tttgatcgtg gagcgggcaa aatacatatt
    14821 ccccactcac tgtcccttca gacaatggtc cttgtttgtg tggacagata ttgtgaatcg
    14881 catgaatttt gccactttct gttaaaaaca accctacctc tttgcctttg acaataacct
    14941 tttttccaat taggggtgtt aattcatcta tagttgtcac tttaattttt tcttttgttt
    15001 ccatgtatta caccttctcc acttcaaaaa ttctacgtgc ttgcgcattg ctagttattg
    15061 cttcccaagg ttcagcttcg actgcttttt tagcatccat aatgcgttca aatagttcat
    15121 tttgtctttc tgggtcaagt aagacttctt ttacattttc aaatccaagt cttcttaacc
    15181 atggcgctgt tctttcagca tatatacctg tttcgcgata atattgcatc aaagcaccac
    15241 ataatttgat tacttcatct tctgtttcta cagttgttaa aaattcagct ttttcaactt
    15301 ctgtaccacc attaccaccg atatagattt ggaatccatt ttcaactgag ataataccaa
    15361 aatctttaac acctgattca acacaacttc ttgggcagcc tgatacaccc attttgaatt
    15421 tatgaggtgt atcgatgtat tcaaatgttt tttctaaacg aatgccaagt cgtgtcgtgt
    15481 attgcgtacc aaatcgacaa aactctttac caacacagct tttaactgag cgtgttttct
    15541 taccataagc tgatgctgaa cgcataccta ggtcttccca tatatttggt aattcttctt
    15601 ttttaactcc atacaaacca acacgttgtg aacctgtcac tttaactagt ggcacatgat
    15661 atttcttagc cacttctcct agacgaatca gttggtctgc atctgtaaca cccccacgca
    15721 tttgaggtat aacagaaaat gtaccatcat tttgaatatt cgcatggtaa cgttcgttag
    15781 caaatcttga ttctctttca tcttcatgat catgtggata aaccatgttt aaataatagt
    15841 tgattgctgg tcgacatttt ggacatccac ctttattttt aaagtttaaa acatgtcgaa
    15901 cttctttaga tgtttttaaa cctttcgctc ttatttgcgt tactatttga tcgcgtgtca
    15961 aatcagtaca accacatata ccagcaggtt ttgcggcaac aaagtcatct cctaaggtgt
    16021 gctgcaatat ttgagcaatt tgcggtttac atttaccaca tgaattcccc gcttttgttt
    16081 tagccgttac ttcttcaact gttgtaaagc cattttccgt aatcgcattt actatagtac
    16141 ctttatcaac accattacaa ccacaaattg tttcatcatc agccatatca gcaattgata
    16201 gcgatgcctc ttctccacct ttagtaagca atgatacaag tgtgtaatct tcagtggatt
    16261 cacctttttt catcatgtta taaaagcgtg aaccatcatc gatatcacca tatagtactg
    16321 caccaactac attaccgtct tttaaaaaga tttttttata gttattatca acactattaa
    16381 atatttcaat acctttaatt tctgcatttt ctacaatttg accagcacta tacaagtcac
    16441 acccagaaac ttttaatgac gtaaatgttg ttgatccctt gtatccgttc gtttctttat
    16501 ttgttaaatg atcagctaat actttacctt gttcatatag tggtgcaacg agtccataaa
    16561 ctttgccgtt atgttctgca cattcaccaa ctgcatatac attgctatca cttgtttgca
    16621 tcacatcatt gacaacaata ccacgattaa catctagacc tgattctttg gctacttctg
    16681 tgtatggtcg tatacctact gccataacaa ctaagtctgc cggaatctcg cgtccatcag
    16741 ccaatttaac accctcaaca tcatcttctc ctaagatttc agttgtgttg gcttgcattt
    16801 caaacttcat accttgcttt tctagatctg ctttaagcat atttccagct ttacggtcta
    16861 gttgcatttc catcaaccat tcagctaaat gtaacaccgt tacttccata ccttgatcta
    16921 ataaaccacg tgcacactct aaacctagta atcctccacc aattacaatt gctttctttt
    16981 tagtcttagc aatgttcatc atttgttcag tatcatcaag cgttctccaa ccaattacac
    17041 tcggtaatgt cgaacctgga actggaatga cgaatgcttt agaacctgtc gcaaaaatca
    17101 atttatcgta tgatacttca ataccatttg cagtagtaac tgattgattt gctctatcta
    17161 cttcaattac aggatcattt gtaattaact cgataccatg ttcctcatac cactcatatg
    17221 gattcataat tgtttcttca actgtcattt tattttgtaa aatatttgaa agcatgatgc
    17281 ggttatagtt tggataaggt tctttaccta ttaccgtaat atcatataaa tcgttggcgc
    17341 gctctaatat ttcttcgatt gttcgaatgc ccgccatacc gttaccaatc attactagtt
    17401 tttgctttgc cataaaatat gcccctttac tccataatat ttatttcaaa aaaaggtatt
    17461 aatttttcgt tagtgctttt atattttcat tggaatcatt aagctttcta atctatcgtt
    17521 aatgatttgc tttaaaattg ggtcgaagtt aattgaaggt gtgaagtgta tatctgtatt
    17581 aataaccatg tcattcattt gctgcttcac tttgttaaca agtcttccgt catataaaaa
    17641 taatggtacg acaatcaatt tttgataccg tttcgagatg ctttctaaat catgtgtaaa
    17701 actaatctct ccatatagcg ttctcgcata tgtcggcttg ctaatttgca aattttgagc
    17761 gcatatttgt aactcttcgt gtgccttagt aaactttcca ttaatattgc cgtgtgcaac
    17821 aaccataact ccaacttgtt gttcgtcacc tgctaatgcg tcacaaatac gttgttcaat
    17881 taatcgtctc attaaaggat gtgtgccaag tggctcgctt acttctacct ttatgtctgg
    17941 ataccgtcgt ttcatttcat gaacgatatt cggtatatcc ttgagataat gcattgcact
    18001 aaagattagc aatggtacaa ttttaaaatg gtcaacccca ctttgaatca acgtcgtcat
    18061 taccgtctct aaatcctgat gctcactttc taaaaacgca atatcatagt gatgtatatc
    18121 atcttttact aattcagaaa taaatgcttc taacgcttga ttctgtcgtc cgtgcctcat
    18181 gccatgtgca acaatgatat tcccattcac atttaccaac cctttcacac gtattgtata
    18241 ccaaatcatt ttgtttttgt gaaaagaatc acattataat gtaaaatcag ggaattccct
    18301 gatgcctgta gtcatgcata ttccttatac attttccctt tttgttaaat caaaaaaagc
    18361 gaccgatata tgaatcccta ctcaacattt atttgagcaa gcattaatat atcggtcgct
    18421 tgtagtgtat attattatct taaaatggtg gttggcctaa tattgtttcg tcaaagcgct
    18481 cgggtatcaa tactttgcgc atgatcacac ctaaatcgcc atcatcattt tcatgttcgc
    18541 tgtatatttc ataacctctt ttttcataaa ttttaagtaa ccacggatgc aatcttgcag
    18601 atgtacctaa agtaactgcc gctgacttta acgtatctcg caaaaatgct tcttcaacat
    18661 aagtaagtaa ttggctacca tagcctttcc cttcatactc aggatttgtc gcaaaccacc
    18721 agacaaaagg ataacccgaa atacttttca cacttcccca aggatatcta accgtaatcg
    18781 tagatataat ttcatcatca attgtcatga caaatgtagt atttttatct atattttctt
    18841 taacagcatc taaattagca ttaaccgaag gccaatcaat acctagttct cttagaggcg
    18901 taaatgcttc atgcatgagt tgttgcaatt tttctgcatc ttgttcactt gcgagtcgaa
    18961 tcatcgtttt tgtcatatta atccccactc ttttttaaat gatttaacca tattttattt
    19021 ttaaaataaa tatccatcaa agtgtatcaa taaatttatc acatgtcaga aagtatgctt
    19081 catctgaata caccaatact ctcatgaaac ttattaaaaa ttactctctc aacgtaaaaa
    19141 accattcaaa ttcatgaatg gtttggaaga atgattcatt gttacgctat ttaatcacta
    19201 catcttaatt attgttgctc taaacgatta cgcttaccat ttaagaaagc ataaacgaga
    19261 cctacaaaaa taccgccacc gacaaagtta cctaagaaag caaaaacgat attttttaaa
    19321 acgtgtaacc atgaaactgc atcaaggtta aagaatacca tacctgcata tagacctgca
    19381 ttgaacacaa cgtgctcata tcccatgtat acaaagacca cgacaccaca agctatgaag
    19441 aatgcctttg ttaagccgcc tttgaattgc atagagatga aaataccaat attaataaag
    19501 aagttacaga aaataccttt tgtaaaaata ttcaaccatg ttgaatcaac agtctttttc
    19561 tgaactaaag ctgttaaagc ttgtgtcatt tcaggtgtca tgacatgcgc aaatttcatt
    19621 aagaaaaata acacaaaccc acctaaaata tttcctaaga aacaatataa taaaatccaa
    19681 gtcatcttct taacagaaac gactttataa taccagccta ctgtaaagta catgaagtta
    19741 cttgttaata attcagagtt agttaataca actaaaatca aacctaaact aaacgcaatg
    19801 gctcccatta aattgataag tccatctacg tgagtactcg caaactgcgt ctttattccg
    19861 aacataaata cagtaacgat tgagagtaaa aatccggcca tcattgcttt aagcatatat
    19921 cggccaggtg tttgctgtac cattacttgc ttcatttgta ctgaagatga tattgaatca
    19981 acaattgcgc ctgttgaata tgtatcttca actgttttct tattttctat cacagtactt
    20041 cccccattta ctttgatcaa ataaaattta ctttctaatt atattagtaa atcaatgtaa
    20101 ttgatagatt aaatttcaga aaaatctttt tattctctcg aaaatgtagt tttctttcat
    20161 aaaaacagcg agataaatcg cgtgattggc taatatcttt atgatttaag atattacgac
    20221 gctcatttta tctcgcttta tgtaaagtat tacttattct tttttaaatt tgaaaagtaa
    20281 tgtaaaccat atgcaaatac actgtaacca attgcagtac gtaaaataaa ccttataact
    20341 ttattaggta cacattttga cgttactgaa atagaagata gtagtgttgt gaaaatatat
    20401 aatattaaaa ctttgatata acgttctacc atcgaatcat ccctcatttt tataatatta
    20461 caagattatt ataaaccgaa tgaaacaaaa aagtttttac atattttcaa caactttatt
    20521 tcaataaagc atcaagttga tctaaagttt gattcatacc ttgttcaaca cccatgttta
    20581 tgacttgttg agcggcttct tttgttggaa ataccgatgt tgatgtcact gtcgttttcg
    20641 ttgtattgct cttggaaaat gacaaagtaa ttttcatgct tggcattttt gtatcttttg
    20701 caccttgagg tgtcgcaaat gagtcaatat attcaattaa atatggacgc ttaaccgttt
    20761 tatattctgc taatgtatag ctggtcattg taggtgtttt aatcgcataa aatgcatcac
    20821 cgcctgaaac agcattaaag cgaaacactt tggtactagc gtcttttgga tgaaaccatt
    20881 tttcaaataa tgcttttgtc gtataggcat caaacacctt ttcaatcggt gcttctatcg
    20941 ttcttgaaaa aataatttta ttatcttcaa ctttaatcgt catcttccca ctccctatct
    21001 tttatattta ctcatcttaa catgcattga gtaacatatg ttaccaaatc atttgttata
    21061 aactatagta atattaatca ttccctctgt tgaaattgat aactacgaaa taaaaaacac
    21121 actctattca gctattaaca gttgagtaga gtgtgcaaac ctgttgaatt aatgcgctaa
    21181 catttcttct ttgatttggt ctttatttaa ttttgaagga taatatgttg gccagttatc
    21241 catttctttt aataacgcgt cattatcatt acccataaag atatggaagt gttctgcctt
    21301 tttaggtgca atgttgtgat cgctaaattg gatgaatttc ggtaagtctt tatcattacc
    21361 atcgaccaat ttaaacataa atctcacgcc acggttacct ttaggatatt tcaatgtttt
    21421 cttaccattg tattcatatt taccagtgtg tttcttacca tctttagtaa atgtaatttc
    21481 atttcctttt atatcaatgt tagtaatatc agttttatat cctttgtcat aataagcttt
    21541 taaatcttta gcagatttct tcggatcatt ttcagcttta tgttccatca cttcatcaag
    21601 cgtaccgtct tttaagtaag ggtaaacaga ttgccattca ccagcataat cgcttaattc
    21661 acggtcttta acttgctcat ctttaaaata cccatcagaa attgctttgt catgtttact
    21721 ttcagctttg tcgtctttca ctttaacacc actgtctaaa gctttaccga tattttcaat
    21781 attagatttc attaatgatt gatacgtaat attatctttt ttctgttgtt ctttatttaa
    21841 agactccatg ttgtagaatt ttaaaggctt cgcatctgtt tctttacgaa ttgtttcagt
    21901 cactttattc gcaacattat cttcgtaaag aatatattta gcattgctat ctctaatttc
    21961 tttaacaatt ttagttaatt ctttttgtga tggatcttca gcattcatgt tttgaatacc
    22021 tttttgaaca aaaccataac aatcagctaa gtatccaatt gattcatgtg aaatgaatac
    22081 tgcattacct tgcttatctt ttgtaacttg cttcatatcg ttatcaattt tcttaagatc
    22141 gtcgtttaat tttttgtagt ttttctcata gtcatcttta tgttttggat cttttttcac
    22201 taattcatct ttaatttctt tagcgaaagt ttggttaatt ttaggatcta accatacgtg
    22261 tggatcatat ccaccatgat gatgatgatg ttcttctttc tcatgatcat gtccctcatg
    22321 ttcatgctct tcaccatgct cgtgttgatc agttaaaagc tttgctttat ctaatttatc
    22381 ctctaaagac agttttttat ctttatcttt gatagtagat gcaactttct tagcaaccgg
    22441 atctaaatta tcccctgtat acataaacaa gtctgacttg cttgcactta atatatcttt
    22501 ttgtgttggt tcatagctat gtaaatctgt ccctgctgga tagattgatg ataccttcac
    22561 gtgttttcca ccaatttgct ctgcaaatga ttgcaatgga taaactgtcg ttttaattgt
    22621 taccttgcca tctttatctt ttccatcatc attcccacat gcggctaatg ataaagttac
    22681 ggctggtaca agaagtaaca tacctaattt ctttttcata gtcaccctcc taaatagtaa
    22741 tctttacgtt ttaaaattat atactcatgc tacttaaaat acaagcactg tttttataaa
    22801 taatataaag tcgaatcata ctttaggtat ttaccacttt tcgttttatc taatacatga
    22861 ctattgtaca aatatgatac agaaaacttt atagtattta cgaacgtcat aatataagaa
    22921 aaaaagacaa tctctatcat attgctaata gaaattgcct tttcatattg tcctaaaata
    22981 acttacccta tttttatagt gtgactttta gatttaataa tggtatttac atgatgctat
    23041 ttttatatga ttgtcatcta ctgtataaac caatctatgt tcgtgattga tacgtctact
    23101 ataataccca gtcagattcg attttaactt ttcaggctta cctatacctt ccaatgcacc
    23161 atttctgtca atacttttaa gtaactcatt aatcttcttc accatttttt tatcgttctg
    23221 ctgaaaatac ttataatctt caaaggcttg aggcgaaaac gtaatattta acctagccat
    23281 tatacatcta tatctttcgt tatagttttc ccacgttcta gatctgcaat tgattgagct
    23341 aagtgttcag cattatttgg gttctgttgg aggtaaagtg tttccatcat ggagttataa
    23401 tctgattctg acatgattac tacgttttta tcatcagtag atgttacagt taccataaca
    23461 ctatcatcat ttacttttgt cataagtgcc tttaaattct gtcgagcgta tgaataattt
    23521 ttaataatca tatttatcgc ctccttttaa cacacctcaa ttgtacaaat atctgtacaa
    23581 taaaacaagc gacacagaaa aaaactacta atcaagattt cgcacgggga agaatcaata
    23641 cctatcctgt actgattccc tactaaacaa tgtttacatg ataatttcca cctatttaga
    23701 cttcatacat taaaaagaca atctctatca tatcgctaat agaaattgcc ttttcgtatt
    23761 gtcctaataa attggtatca cttactgatc tcaacaatta agactatttg attttatctt
    23821 ttaataactt ctcataactt tcataatcat atggatcttc aactttctcg ccattaataa
    23881 atgcagttgg tgtcgttttt atatgattat ctttcgctat ttttttatct ttctctgcag
    23941 ctttccaaga cttgctatct tttgtcttgt aatcttttat aattttattt tccgtctctt
    24001 tatctaaatg cagttgttta atatgtttat ctaatagttc ttttgttaac cattctttat
    24061 tttcatcttg ctgggcagca aataattgct tttgaaaatc taaaaatgat ttaggtgcat
    24121 acatcaatac tgcatgactc gcacgcgaac caacaattga gtctttacct aagaaagcta
    24181 aattgacaaa ttggtattcc actttgtgat tatctatata atttttacgc aactttggca
    24241 tgactttttc atctaattct ttacaataag gacatttata gtcgccatat acgacaacta
    24301 atggtttgcc gtttttcgaa gatgtcgttg ctgattcttt tttaccgcaa gctgttaaaa
    24361 ttaacatgct cactataaat aatgttagta attttttagt cataataact cctattcata
    24421 tctagttcgt catattttct tcatgataat ccgcatcatg ttgttgataa tatcgtctcg
    24481 aatcaaaatc ttgcttatat gatacacgtt tatcactaaa atcatatgca taataatgca
    24541 cttcatcatt actcaaaggt ttataagcta aaatgataat tttatcctta tcatagacat
    24601 aaatattagc atctttgcgt tcaaaatgat caacacgatc accgttgcct tttttggcaa
    24661 gttgttcttg tttttgattt tgaattttaa ctgcttcatc tatttctttc acatatttat
    24721 cattaccaca tcctgatata aagactagta ataataatac cgtcgccaca aatcgcttca
    24781 atgctcccac tcctactatc aacttccaac taactttcaa tataattaat actatacacc
    24841 gtcaaacatt aatgcgctac aactactacc aatgcattta atataaatat ccaaagaaat
    24901 aacagttatg tatagtcaaa gaaaaatata tttcattttc tatgtgtatt tgtaatcagt
    24961 ttttgataaa ataaaaaaat agcattatta ataaattcaa aaattatagt tcaattcaga
    25021 gagggatggg ataaaatgaa ctttgtaacg ttgacttcgg atgagttcaa tgcgtttaca
    25081 acaaagcatt tttcacatta cacacaatca gctattcatt acaatcatag agttgattta
    25141 aaaggcgatg tgcatcttgt aggggttaaa gatgacaatg gtcaagtgat tgcaggatgc
    25201 ttattgacag aagcacgcac acttaaattt ttcaaatatt tttatacaca tcgcgggcca
    25261 gtgatggatt atacaaatca atcattagta gcatttttct ttaaagcatt aacgtcatat
    25321 ttaaagaaac acaattgttt atatgtcctt gtagatccat atttaattga aaatttacgc
    25381 aatgcagacg gtgaaattgt taaatcttat gataaccgag catttgttag aacaatggat
    25441 aaattaggtt ataaacacca aggtttccct gtaggttatg attcaatgag ccaaatccgt
    25501 tggctgtcag tgttagattt aaaagataag actgaagacc aacttttaaa agaaatggat
    25561 tatcaaacga gacgtaatat taaaaaaaca tatgatattg gtgtcaaaac taaaacgtta
    25621 acgattgatg aaacgcaaac ttttttcgac ttattccata tggctgagga aaagcacggt
    25681 ttcaaattcc gtgagttacc atactttgaa gaaatgcaaa agttatacga tgaccacgcc
    25741 atgttaaagt tggcgtatat tgatttaaac gagtatttaa aaacgttaca attaaagcaa
    25801 caacaattaa cagctgaact ctcaggtgtt gaagaagcat tggaagaaag tcctaattca
    25861 aagaaaaata aaacgaaacg cacacagcta gaacaacagc taaatagcaa taagcgtaaa
    25921 atcgacaata caatagaaca aattgaacaa gatggtgcag tcttaaactt agcttctgct
    25981 ttatttatct acaatgagca tgaagtttat tacttatcta gtggttcaaa ccctaaatat
    26041 aatgcttata tgggtgccta ccatttgcag tgggaaatga ttaagtttgc taaagcgcat
    26101 catattgatc gctataattt ttatggtatt accggtgact tttcagagtc atcagaagac
    26161 tatggcgtcc aacaattcaa aaaaggtttt aacgcacatg tagaagagta tatcggtgac
    26221 tttattaagc ctatcaaacc attactatat aaggtacaaa cgtacttaaa tcataaacgt
    26281 cgttaaaata tcgctccaag attatgacaa cataacaaag aaccacgcat catgtatatg
    26341 gcttcatcgc catactaaaa catgatacgt ggttctattt gattattctt catttataac
    26401 atttaagaaa cgtcttaatt cttctgtttt cggacgattg aacatctctt ctggagcccc
    26461 ttgttctccg atcatgcctt catgaataaa tacaatgtta ttagatactt ctttagcaaa
    26521 acgcatttca tgtgtcacaa tgaccattgt catgccttca ttagccaaat ctttaataac
    26581 ctttaaaaca tcattcacaa gttcaggatc aagtgcagat gttggttcat caaacaacat
    26641 cactttaggg ttcattgcta gtgctcttgc aatagcaaca cgttgttgtt gaccacctga
    26701 taatgcatgt ggacgttgat ctttgacatg tgttaaacca actttctcaa gtaactcaag
    26761 tgatttccca cgtgcctcat cctttttcaa ctttttaact gtgataagac cttccattac
    26821 attttctaat gccgtcttat gcggaaaaag gttataactt tgaaatacca taccagactg
    26881 tttacgaact tctatttgtg attttttatc tttagatgta tatgttttgc cgttaacata
    26941 aactgtacct tcagttggaa tttctaatgc attaatcata cgtaacaatg ttgttttacc
    27001 tgaaccagat cgaccgatta aggttacaac ctcaccttgt tcaacagata aatcaatacc
    27061 tttgatgact tcaacatcat taaatgattt atggatattg ttcaattgaa tcatgagcga
    27121 taccctcttt caatataaga ttcatagata ccttggataa ttgaaataat aaagcatacc
    27181 acccagtaca ttagcgcaac taacacataa attgttaaat attcatatgt tgtagacgca
    27241 acttcttgag cttttctaaa catttcagcc actaaaataa atcctaataa tgatgtatct
    27301 ttgattaaac ttaaaaatgt attacctagt gcaggaatcg acactcgaat tgcctgcggt
    27361 aaaatgatac gttgtatcgt ttgaccatac gtcataccga tggaatatgc agcttctgtt
    27421 tgtcctttcg gtatagaaat aataccgcca cgaataattt ccgaagcata cgcacctaca
    27481 tttaatgata aaccaataat agcagccact acaggtgcca atgtccattg gttgtcagcg
    27541 tcatttgtta ataatctacc taattctgga ataccataaa atatgataaa tagttgtact
    27601 atcattggtg ttcctcgaat aatagataca tagacacgtg aaatacttct taaaatttta
    27661 ctcgttgaaa ttcgcattaa tgctgtaaat aatgcaataa tcaatcctaa aacaaatgta
    27721 actaatgtaa taggaattga atatttgacc aaaccttcta gcataggtcc gaaagcttgt
    27781 tttgcagcat ctagcgcatg ttgttgttcg ctatttagat ttagaaacat cttgaccaaa
    27841 ccatttctta cctattttag ctaattcacc gttttcctcg atttttttca agccatcatt
    27901 gaatttttga actgtttcat catctgcttt tttagaaaat gcaaatgcag atctactttg
    27961 ttcagcatta cctttgattg ctttgatctt agcattaggt ttttgttttt tataatccaa
    28021 gtatgacaga ctatcattaa atgtaccatc aacacgctta gacaataata aatccattga
    28081 ttggttaaag ccatcaactt ttgtaatatc agcaccttta tccttagcta atttaccata
    28141 attagatgtg aatgtttgtg ctaacttctt acctttaaca tcatcaaaat ctttaatatc
    28201 tttttcattt tcacgaataa ctaaaacagc acttgagaat gtgtaaggct tagaaaattt
    28261 atatttcttt tctctatcag gattaatacc tacttggttc gcgattacat caaaacgccc
    28321 tgcgtctaaa cctgcaaaca tagaatccca agaagtttca ttaaatttaa gttttaaacc
    28381 ttcttcttta gccactgctt taataacatc aatatcgtaa ccagttaatt taccatcttt
    28441 atcgtggaaa ctaaatggtg cataagtccc ctcagtccca acattaattg tcttgctatc
    28501 tttactagct tccttgtctt tcgacgaatt gtttccgcat gcagccaata tgaaaacgaa
    28561 agctatcatc acaaataaaa gtcttttcat agaatatatc tccttattct tattattcta
    28621 atcggaataa aaatatttta atctgaaatg ccggataagt caatatgttg aacttttgct
    28681 aaaaagcttt atatagctag caacttatat ttattgacga gaatcaactt cttttttctt
    28741 accttgtaac ataaatgaac aaaagaaact aacaactgta atcaataatg caaataaaaa
    28801 tccagcgtga taaccatgca acatcgtatc aactttaatt tgattcatca tatcaatttt
    28861 gttcatgcca ttatagtctg acatagttgg agaaaagttt ttgcttgcat gggataaaat
    28921 agtaattaat gcagctgttc cgatagaacc agcaatttgt tgtactgtgt ttgtcataga
    28981 tgaaccatgt gcattcattt ctggctttaa ttgattcatc gtatgggtca ttactggaat
    29041 taatcctaac gcgataccaa ccattcgaat cgcataaact gttgccaaca tgattgttga
    29101 tgtttgttca tccattacaa caaaataagc cgtagtaata acaacaatcg ccatacctac
    29161 taaagcaaga tttctaggac caaacttttc atataaagca cccgaagtca tcgacataaa
    29221 tgccataaca attgctcctg gtaataaagt taatccagaa tctaaagctg aacgatgcaa
    29281 taaattttgc acaaagatag gtaaaatcgt ttccgaacca atcatcgata ccattgtgac
    29341 acccataata atgataccaa ctgtaaattg tttattttta aatacagcaa aatttaataa
    29401 tggtgttgat agccgatatt gacgtaatac aaataatgcg actaaaacaa cgcctccaat
    29461 catagatact aatacgatag gattatcaaa acctttttct gaaattgaac tcgttccata
    29521 caataacccg ccgaaaccta gtacagaaaa aatgacagaa ataaaatcaa taggtacttt
    29581 aattgtagtt ccaacatttt ttatcgtttt aaatccaatt aaaaatgtca cagctgcaat
    29641 tggagcgaca acatggaaaa gcgatctcca gttaaaatat tcaacaaggt aacctgaaag
    29701 agtaggtcca atagctggtg ctaaaccaat taccaaacca aatatcccca tatatttacc
    29761 gcgttcatgt ggttggaaaa catccaaaat cgttgtcatc atcaatggca tcataatacc
    29821 tgcgcctaac gcctgaataa ttctagcaac taataatgtt gcaaagtgcg gactcaagcc
    29881 gccgagtaat gtacctaaga aaaagataga aatacccact aaaaacactt gtcttgttgt
    29941 ataacgttgt ataacaagcg cggacaaagg aataacaatc ccattagtca ataaaaaagc
    30001 tgttgtcagc cattgaactt gtgtatatga aatttcaaaa tctttcataa tacttggtaa
    30061 agccgtcact aatagtgttt catttaataa tccaaaaaat ccacccatca gcatagtaat
    30121 aatcatcgtt aatttttgtt tcttcgacat ttaaagcttc cccctcacca tctaaaaaac
    30181 gacacacatt tttagatatc cggtaattat acccattttc caaaaaatta gcacgacttt
    30241 attacaattt ctttaaaacg taatcattac tatttacata tgagtagtta tcggtcataa
    30301 tatatttaca aggagggata gagatgaaat tagatttaca gactgctcgt cgtaatttaa
    30361 atagccctaa cattaaaaca agaaagcgtg ctttaaagat tattaagcaa cataaaagag
    30421 cgaaataatt gttttaattt gactatattg tatgtctaac tatattttcg agtatcccta
    30481 gctcgttaat ataaactcta tacttaacta tttccttaca ttacttcaca tatttaagag
    30541 ctgcatatac actcactcct tgtgtacatg cagctctttt tttataagta gtatttatct
    30601 ataacttcta aatcatccgt taattcataa acaagcggtg cacctgtttt aatttcataa
    30661 ttaatgatat cttcatctga cacatcttca agatatttaa tcaatgcgcg aattgaattt
    30721 ccgtgtgcag aaactaatac cgtttgacca tctagcaaat attgtgaaat atgatctgtc
    30781 caaaatggta tcactcgaac taaagtatct ttcagacttt cagaataagg catcatacgt
    30841 ttatctaaat gattatatcg acgatcagct aagtaagctt cacgttgttc ttcggtttca
    30901 gcaggtggtt tcacatcata agaacgacgc caaatatgta cttgttcttc tccaaattct
    30961 tttctagcat catctttatt taagccttgc aatccaccat agtggcgttc atttaaacgc
    31021 cagcttttat atacaggaat ccattgttgt ttagattcag ttaaaatata atgcgttgta
    31081 tctaaagcac gtgttaataa cgatgtaaaa gctacatcga tggcaatgtt attttctctt
    31141 actttttcac ctgctctagt cgcttcatta ataccttgtt cagataaatt aacatcttcc
    31201 catccagtaa ataagttttt agcattccac tcgctttgtc catgacgaca taaaattaat
    31261 tttggcataa cttataacct ctttccttta atctttagga cgttatttca gggatatttt
    31321 aagatgattt aatcttagta attttctttt caatatattt taaaattcaa tgattaaatc
    31381 aattatcact agacattaaa tacataaatc ctattcccca ttttcatttt ttaattcata
    31441 aatgaatcaa taaccaccta ataacaaatc atattataca cctttgttcg ctatttttct
    31501 aaggtttaaa aaatattttt aggtaaacct aaaaatagat gtaataaaaa cgcctcctca
    31561 gatatttata tatctatgaa gacgtttaaa tacattatag atggtctggt tctgggtgaa
    31621 cgtatactga ggaaatacct tttttgtgca aatgatgttc gacattgtca caaatttgat
    31681 gcgcttctac taaggaaagg ttagcatcta caacaattgt gacatcaata aacacactac
    31741 ttccatggta acgcccttta atacttttaa cttcttgtac ttcatcaact tctaaaatat
    31801 cattgcgata cgcttctaat tcagtttcat tgaaaccatc actcaacata aaaattgctt
    31861 ctttaaaaat accaaaacca gtataaacga ttagtaagcc tagtaatgtt gctaaaataa
    31921 tatcgacaat tgggaaaccg atttgcgtaa aaattaatcc tatcgctgtt ccaatgctga
    31981 ctaaactatc cgataaatta tctttggcag ccgaatttaa agaactactt ttcgttcttt
    32041 tcgctagtct ttgattgact gcaaatacaa tcaacattac aagaccactg attaagctga
    32101 cgataattgt tattgcgtta ggtacaacgt catcttcttt gaacaaacga ggtgcatttt
    32161 gaataactac ttggatacct acaaacataa tgacaaatga caccaataat gaagaaatat
    32221 tttcagactt caaatggcca taaggatgat ttcgatcggc aggtttaatt gaaattttca
    32281 atccaataat aacagctaaa gaaacgataa tatctgtcat attgtttaat gcatcggctc
    32341 ttacagctgc agagttaaag acaaaacccg tgacatactt aacaatagat aagattatat
    32401 atacaattaa actcaaataa gcaccgcgtt gcgccaattt aagattttca ttatgagaca
    32461 tgcgttgaac caccttgaat tagtatagta acaatattat gaatgattca ttttaatttt
    32521 acaacgtttt taatttttat aaatttttat aaaattaaac taatttattc attcgcaacc
    32581 cctaaaaata atttttagcc tttctgcgaa ttttatgagc tagaaaggcg cccaactctc
    32641 cctgtttgtt aactttcgcc tcgaaagttt ctatgttggg accctatgca ttagtagcga
    32701 agctatctaa tgtgttcctt tattttctgc gaattttatg agctagaaag gcttatgcag
    32761 ttgacgtttt acgtccaact gcgttcctcc gtcttcttca aatttatttg ttagaaaggc
    32821 acccaactct ccctgtttgt taactttcgc ctcgaaagtt tctatgttag aaccctatgc
    32881 atgagttgcg aagctatcta atgtcgtgaa ctaattatat agaagaaaaa gtgcatcaat
    32941 gacaaattaa atgagatttc tactctacca aactctcttc gaaagacaat tttctcctct
    33001 atttattagc aaactattgc atttctccat atagtacttc cttacttaaa atacgctgaa
    33061 tgttctgaat taaacgcttt tttacatagt taacactagt taatctatta gttaacatta
    33121 gttaataatt agttaatttc catttgtatt ctcatgtgat aaattctaaa agcatacaat
    33181 aaatttaata tgtaaaaaga aagggaatac acatgaaaaa taaatatatc tcgaagttgc
    33241 tagttggggc agcaacaatt acgttagcta caatgatttc aaatggggaa gcaaaagcga
    33301 gtgaaaacac gcaacaaact tcaactaagc accaaacaac tcaaaacaac tacgtaacag
    33361 atcaacaaaa agctttttat caagtattac atctaaaagg tatcacagaa gaacaacgta
    33421 accaatacat caaaacatta cgcgaacacc cagaacgtgc acaagaagta ttctctgaat
    33481 cacttaaaga cagcaagaac ccagaccgac gtgttgcaca acaaaacgct ttttacaatg
    33541 ttcttaaaaa tgataactta actgaacaag aaaaaaataa ttacattgca caaattaaag
    33601 aaaaccctga tagaagccaa caagtttggg tagaatcagt acaatcttct aaagctaaag
    33661 aacgtcaaaa tattgaaaat gcggataaag caattaaaga tttccaagat aacaaagcac
    33721 cacacgataa atcagcagca tatgaagcta actcaaaatt acctaaagat ttacgtgata
    33781 aaaacaaccg ctttgtagaa aaagtttcaa ttgaaaaagc aatcgttcgt catgatgagc
    33841 gtgtgaaatc agcaaatgat gcaatctcaa aattaaatga aaaagattca attgaaaaca
    33901 gacgtttagc acaacgtgaa gttaacaaag cacctatgga tgtaaaagag catttacaga
    33961 aacaattaga cgcattagtt gctcaaaaag atgctgaaaa gaaagtggcg ccaaaagttg
    34021 aggctcctca aattcaatca ccacaaattg aaaaacctaa agtagaatca ccaaaagttg
    34081 aagtccctca aattcaatca ccaaaagttg aggttcctca atctaaatta ttaggttact
    34141 accaatcatt aaaagattca tttaactatg gttacaagta tttaacagat acttataaaa
    34201 gctataaaga aaaatatgat acagcaaagt actactataa tacgtactat aaatacaaag
    34261 gtgcgattga tcaaacagta ttaacagtac taggtagtgg ttctaaatct tacatccaac
    34321 cattgaaagt tgatgataaa aacggctact tagctaaatc atatgcacaa gtaagaaact
    34381 atgtaactga gtcaatcaat actggtaaag tattatatac tttctaccaa aacccaacat
    34441 tagtaaaaac agctattaaa gctcaagaaa ctgcatcatc aatcaaaaat acattaagta
    34501 atttattatc attctggaaa taatcaatca aaaatatctt ctctagtttt acatcatttt
    34561 ttaaataatt ttcgtaacaa accgtgatta aaaagaaccg ttgattctca atcgaatcta
    34621 cggttctttt ttcattttcc atcaattaaa tgcttcttcg ctatttgtca gcccactttt
    34681 ttacctgcaa cttgttaaat aatccttaca tcgttaacga atagttcatc atttagttga
    34741 atcagctcaa ctttattaac ttcatatttt cacaaactat tgcgcaatcc attccttttc
    34801 cactacaagc accataatta aacaacaatt caataaaata agacttgcaa agcatagtta
    34861 tgtagctata taaacgcctg cgaccaataa atcttttaaa cataacataa tgcaaaaaca
    34921 tcatttaaca atgctaaaaa tgtctcttca atacatgttg atagtaatta acttttaacg
    34981 aacagttaat tcgaaaacgc ttacaaatgg attattatat atatgaactt aaaattaaat
    35041 agaaagaaag tgatttctat gattaaaaat aaaatattaa cagcaacttt agcagttggt
    35101 ttaatagccc ctttagccaa tccatttata gaaatttcta aagcagaaaa taagatagaa
    35161 gatatcggcc aaggtgcaga aatcatcaaa agaacacaag acattactag caaacgatta
    35221 gctataactc aaaacattca atttgatttt gtaaaagata aaaaatataa caaagatgcc
    35281 ctagttgtta agatgcaagg cttcattagc tctagaacaa catattcaga cttaaaaaaa
    35341 tatccatata ttaaaagaat gatatggcca tttcaatata atatcagttt gaaaacgaaa
    35401 gactctaatg ttgatttaat taattatctt cctaaaaata aaattgattc agcagatgtt
    35461 agtcagaaat taggctataa tatcggcgga aacttccaat cagcgccatc aatcggaggc
    35521 agtggctcat tcaactactc taaaacaatt agttataatc aaaaaaacta tgttactgaa
    35581 gtagaaagtc agaactctaa aggtgttaaa tggggagtga aagcaaattc attcgttaca
    35641 ccgaatggtc aagtatctgc atatgatcaa tacttatttg cacaagaccc aactggtcca
    35701 gcagcacgag actatttcgt cccagataat caactacctc ctttaattca aagtggcttt
    35761 aatccatcat ttattacaac attgtcacac gaaagaggta aaggtgataa aagcgagttt
    35821 gaaatcactt acggcagaaa catggatgct acatatgctt acgtgacaag acatcgttta
    35881 gccgttgata gaaaacatga tgcttttaaa aaccgaaacg ttacagttaa atatgaagtg
    35941 aactggaaaa cacatgaagt aaaaattaaa agcatcacac ctaagtaaac agttcaatca
    36001 tcttaaaaaa tcctgggaca cttcatactt gtctcaggat tttttaacaa attgaatcag
    36061 ccttataaca ttaaattatt ttatcgtaca ttaaatttaa taataacaac tgatttttat
    36121 aagaataaag tatcgaacca
//
LOCUS       DALAAR010000024        34025 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000024 DALAAR010000000
VERSION     DALAAR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.024"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            318..671
                     /locus_tag="O2458_001179"
     CDS             318..671
                     /locus_tag="O2458_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ3887049.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            739..1134
                     /locus_tag="O2458_001180"
     CDS             739..1134
                     /locus_tag="O2458_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ3887050.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1394..1963
                     /locus_tag="O2458_001181"
     CDS             1394..1963
                     /locus_tag="O2458_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3887051.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(2090..2290)
                     /locus_tag="O2458_001182"
     CDS             complement(2090..2290)
                     /locus_tag="O2458_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ3887052.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(2683..2874)
                     /gene="vraH"
                     /locus_tag="O2458_001183"
     CDS             complement(2683..2874)
                     /gene="vraH"
                     /locus_tag="O2458_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCZ3887053.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            complement(2903..3229)
                     /locus_tag="O2458_001184"
                     /pseudo
     CDS             complement(2903..3229)
                     /locus_tag="O2458_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3244..3330
                     /locus_tag="O2458_001185"
                     /pseudo
     CDS             3244..3330
                     /locus_tag="O2458_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3332..3829
                     /locus_tag="O2458_001186"
     CDS             3332..3829
                     /locus_tag="O2458_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ3887054.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(3889..4389)
                     /locus_tag="O2458_001187"
                     /pseudo
     CDS             complement(3889..4389)
                     /locus_tag="O2458_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4437..4628)
                     /gene="vraH"
                     /locus_tag="O2458_001188"
     CDS             complement(4437..4628)
                     /gene="vraH"
                     /locus_tag="O2458_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCZ3887055.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(4721..6601)
                     /gene="vraE"
                     /locus_tag="O2458_001189"
     CDS             complement(4721..6601)
                     /gene="vraE"
                     /locus_tag="O2458_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCZ3887056.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(6591..7349)
                     /gene="vraD"
                     /locus_tag="O2458_001190"
     CDS             complement(6591..7349)
                     /gene="vraD"
                     /locus_tag="O2458_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCZ3887057.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7632..8144
                     /locus_tag="O2458_001191"
     CDS             7632..8144
                     /locus_tag="O2458_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCZ3887058.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(8278..9072)
                     /locus_tag="O2458_001192"
     CDS             complement(8278..9072)
                     /locus_tag="O2458_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCZ3887059.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(9315..10331)
                     /locus_tag="O2458_001193"
     CDS             complement(9315..10331)
                     /locus_tag="O2458_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCZ3887060.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(10496..11071)
                     /locus_tag="O2458_001194"
     CDS             complement(10496..11071)
                     /locus_tag="O2458_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ3887061.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            11296..12204
                     /gene="rarD"
                     /locus_tag="O2458_001195"
     CDS             11296..12204
                     /gene="rarD"
                     /locus_tag="O2458_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCZ3887062.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(12617..14035)
                     /locus_tag="O2458_001196"
     CDS             complement(12617..14035)
                     /locus_tag="O2458_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCZ3887063.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(14273..14737)
                     /gene="bstA"
                     /locus_tag="O2458_001197"
     CDS             complement(14273..14737)
                     /gene="bstA"
                     /locus_tag="O2458_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCZ3887064.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            14904..15242
                     /locus_tag="O2458_001198"
     CDS             14904..15242
                     /locus_tag="O2458_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCZ3887065.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            15232..15999
                     /locus_tag="O2458_001199"
     CDS             15232..15999
                     /locus_tag="O2458_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887066.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(16118..16756)
                     /gene="pcp"
                     /locus_tag="O2458_001200"
     CDS             complement(16118..16756)
                     /gene="pcp"
                     /locus_tag="O2458_001200"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCZ3887067.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(16908..17864)
                     /locus_tag="O2458_001201"
     CDS             complement(16908..17864)
                     /locus_tag="O2458_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCZ3887068.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            18108..19079
                     /locus_tag="O2458_001202"
     CDS             18108..19079
                     /locus_tag="O2458_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCZ3887069.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(19228..19743)
                     /locus_tag="O2458_001203"
     CDS             complement(19228..19743)
                     /locus_tag="O2458_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCZ3887070.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            20123..20971
                     /locus_tag="O2458_001204"
     CDS             20123..20971
                     /locus_tag="O2458_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCZ3887071.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            21000..21554
                     /locus_tag="O2458_001205"
     CDS             21000..21554
                     /locus_tag="O2458_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCZ3887072.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            21607..23319
                     /locus_tag="O2458_001206"
     CDS             21607..23319
                     /locus_tag="O2458_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887073.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            23316..24149
                     /locus_tag="O2458_001207"
     CDS             23316..24149
                     /locus_tag="O2458_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ3887074.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            24196..24693
                     /locus_tag="O2458_001208"
     CDS             24196..24693
                     /locus_tag="O2458_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887075.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            24811..25935
                     /locus_tag="O2458_001209"
     CDS             24811..25935
                     /locus_tag="O2458_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCZ3887076.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            26240..27058
                     /locus_tag="O2458_001210"
     CDS             26240..27058
                     /locus_tag="O2458_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCZ3887077.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            27076..27690
                     /locus_tag="O2458_001211"
     CDS             27076..27690
                     /locus_tag="O2458_001211"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCZ3887078.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            27683..28933
                     /locus_tag="O2458_001212"
     CDS             27683..28933
                     /locus_tag="O2458_001212"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCZ3887079.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            28949..29962
                     /locus_tag="O2458_001213"
     CDS             28949..29962
                     /locus_tag="O2458_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCZ3887080.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            29931..30509
                     /gene="hisB"
                     /locus_tag="O2458_001214"
     CDS             29931..30509
                     /gene="hisB"
                     /locus_tag="O2458_001214"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCZ3887081.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            30506..31084
                     /gene="hisH"
                     /locus_tag="O2458_001215"
     CDS             30506..31084
                     /gene="hisH"
                     /locus_tag="O2458_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCZ3887082.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            31077..31781
                     /locus_tag="O2458_001216"
     CDS             31077..31781
                     /locus_tag="O2458_001216"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCZ3887083.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            31778..32536
                     /gene="hisF"
                     /locus_tag="O2458_001217"
     CDS             31778..32536
                     /gene="hisF"
                     /locus_tag="O2458_001217"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCZ3887084.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            32533..33165
                     /locus_tag="O2458_001218"
     CDS             32533..33165
                     /locus_tag="O2458_001218"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCZ3887085.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            33358..33537
                     /locus_tag="O2458_001219"
     CDS             33358..33537
                     /locus_tag="O2458_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887086.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT        11702 a         5132 c         6133 g        11058 t
ORIGIN      
        1 atgttctatg ctctacgaag ttatattggc agtagttgac tgaacgaaaa tgcgcttgta
       61 acaagctttt ttcaattcta gtcaaccttg ctggggtggg acgacgaaat aaattttgcg
      121 aaaatatcat ttctgtccca ctccctaatt tgagctggat atactttcat ttgaaccctt
      181 tattgctagt ttatgaaagt atcatgaaag ctttatgaac atcgcttgag ttgcctttac
      241 agtagaaaat ttaagtttta cactttgtgt gaatgatacg ttttgtattg aattaattat
      301 agaaaggtac gttgaagatg ttttcaattg gaagtgcaat tcttcatttt gtcattggtg
      361 gtatcgctgt tgcattagct tcaattattg ctgataaggt aggtggtaag ttaggaggta
      421 ttatagctac tatgccggca gtctttcttg cggctattat cgcattagct ttagatcatc
      481 gtggtacgca attagtggag atgtcgatga atcttagtac tggagcaatt gtcggtattc
      541 tgtcttgtat attaactgta tttttgacat ctctctacat taagcataaa ggttatcgga
      601 aaggcgcaat attcacagtt gtttgttggt ttgtcatttc cctcgcaata ttcagtatta
      661 gacatttata gtttggaaaa tgcgtgataa ttagttgtat tcagttatta agtaataaat
      721 tattggaggc agaacatcat gaaattaaca ttaatgaaat tttttgtggg gggatttgca
      781 gtattattaa gttatattgt atctgtaaca ctaccttgga aagaatttgg cggtatattt
      841 gcaacgtttc cggcagtatt tttagtgtct atgtttatta caggtatgca atatggtgat
      901 aaagtcgctg tgcatgtaag tcgtggcgca gtgtttggta tgacaggggt attagtttgt
      961 attttagtta catggatgat gttacatatg acgcacatgt ggttgattag cattgttgtt
     1021 ggtttcctaa gctggttcat cagtgcagta tgtatttttg aagcggtaga atttatagca
     1081 caaaaaagat tagaaaagca tagttggaaa gctggaaaat cgaatagtaa atagtgtgaa
     1141 cgtaatctct taactaggac taactttgca agcattgaat agcatggaaa agttgcatca
     1201 ttaataagtg aaattcaagt tggcattgag aaaattacaa gcgcgtaatc atacaggtct
     1261 gtcttaaggg agtcttcgaa ccccgatgtt gtcgtatgtc aaaacattta gtcaatcata
     1321 aaggtgactt gatttaactt tatctgatag tctgattgta atgattgtac taattgactg
     1381 gaggcgtatg taattgaatc tgagtaaaca aattaaaaag tatagggaac gagatggtta
     1441 ttcacaagaa tatcttgctg aaaagttata tgtatctagg cagagtattt ctaattggga
     1501 aaatgacaaa agcttaccag acatacataa cttattaatg atgtgtgaat tgttcaatgt
     1561 aactttagat gatttagtaa aagggaccat tccatttgta cctgatatta aagcgcaacg
     1621 aagtcttaac ttatggacat atgtgatgct tattttcatg acattagctg caattttaat
     1681 gggaccttta gttgtttatt ggaattggac ttggggtgta acggtggcaa tcattttggg
     1741 aataggtttt tatgcatcta tgaaaataga agatttaaaa aaagtgcata aaatggacaa
     1801 ctacgatcga attgttgctt ttatgaatgg aaaagatcct agtgaagtac aaacgactaa
     1861 agctagaaat acgatgacaa atgcgctttc tattatatca gtaattggta tactcagcct
     1921 cataattttc cttagtgtgt atttggcaaa taagttttta taaatcatcg tggtatcgtc
     1981 tcatattatt tatattatcc aaaatagcat aaaaaaatac caacaagatt tagaaccttg
     2041 ttggtaatca aagcgattca tttataatga gtcgttttat gttgtaagat taaacagttt
     2101 gtacgttaac tgcttggtct ccacgttgac cttcagtgat ttcgaaagta actttttgac
     2161 cttcttctaa agttttgtag ccatcgctag cgatacctga gaaatgtacg aatacgtctc
     2221 cgccattttc ttgttcgatg aaaccaaaac ctttttctgc gttaaaccat tttactgtac
     2281 cgttattcat atagaaaacc tccgtgtgct tttgcactta atatttgtaa caaattcata
     2341 actaaaaaag aggatattct aaacaaatac actacaattt aattcacgag cttttattac
     2401 gtaagaccaa ctatacgctc atattggcat aatgtacagt gttttttgaa aataaattaa
     2461 aaaagatttt taaaaacctt agaaacgttg atttaaaggg gtttataaaa ataaaaaaat
     2521 tgtagtcttt tatggtgttt gctagttttc aaagtgacat atcgtttaaa catgatgatt
     2581 ttataagcaa tccataaaaa acaagcagcg ataaacgcta cttgttgata ttaaaatctg
     2641 acttgaaagg tcatagcaat gttctatacc gatggaatgt gcttacttgc ctttttcttc
     2701 acgacgtttt aaataataag agccacctaa taaaccagct ggaatgccta tcattggtgt
     2761 tgtgaatgag cttaatacaa taacaagtat tgttaaagca atgacgttat accaagttac
     2821 agtcaaattt ttcaaatcct catatgattg ttttactaat tctctaaatt tcatgattca
     2881 atctctcctt ttttataaat ctttagattg tcaaattaag ctggacaagt ttatcatctt
     2941 cgatgaaact cataaatact tccaatggtg ttctataacc taaagatttt ctaggatgat
     3001 gattgatttt ttgagcaaca ctgatgatat gatcatccgt tactaaattg aaatctagtt
     3061 ctttatctaa tccactgcgt ctaattattc cgttagaatg ttcatttaaa cctcgctgtg
     3121 atggcgtgcc tggatcagca aaaaatatag atatatcgtg acagttacat attgttttcc
     3181 agtttgagaa ctccttacca caatcaaaag taattgattt aaatatatga gaagataaaa
     3241 tgaaaaggtg caagcagtat taagaattta ggtagcaaga aaacaagaag tggtacatac
     3301 gatgctaacc taaaaagaat tggagattaa aatgattatt aaccttgaaa cacaacaaat
     3361 acattttgat ttagaacatg aaattcccta ttttcaagca cctgagaaaa ataggatacg
     3421 cttagatatt gatgttctca ataaaaagca aatttctaat attattaatg ttatatttaa
     3481 taaccaatca aagacgaaat gtacattttt gtctgaatac ttatatcctg ttaaatttag
     3541 agaaaaaaca agaattggtc gtttttttaa tattactaac tggtacgaag aaatccattc
     3601 taccgatgag aagtatgtta ttgcaactat taaaaatata aaaaacaaag acttaattga
     3661 ttattcttta tatattaaaa aaggtttagt ggagccattt attgtattct ataatcaaga
     3721 aaatatattc tacattagca atgctgttat agatatcatc agtgtatcca aagatactct
     3781 tgattatata aagtcagaat tcaatgatca ctgtgcttcg ccatactgat tttatagcac
     3841 agagaaaagc cgtagtttcg aagagaccaa cggctttttt ataaatcttt agattgtcaa
     3901 attaagctgg acaagtttat catcttcgat gaaactcata aatacttcca atggtgttct
     3961 ataacctaaa gattttctag gacgatgatt gattttttga gcaacactga tgatatgatc
     4021 gtctgttact aaattgaaat ctagttcttt atctaatcca ctgcgtctaa gtattccgtt
     4081 agaatgttca tttaaacctc gctgtgatgg cgtgcctgga tcagcaaaaa atatagatat
     4141 atcgtgacag ttacataatg ttttccagtt tgagaactcc ttaccacaat caaaagtaat
     4201 tgatttaaat atatgagaag gtaaagatga aaacattgag tgtagagcgt tttctatatc
     4261 agttgcctta cgacctaagg gtcgtaaggt gataatcatc ttggatactc tttctaccaa
     4321 agtaatgatt gcactaccat gacgacgacc aacaatagta tctccttcaa gatgtccaaa
     4381 ctccttatgt tttctttggt aggaaataca tattgagttt agcacataag gcttatttat
     4441 ttaccttttt cttcacgacg ttttaaataa taggcgccac ctaataaacc agctggaatg
     4501 cctatcattg gtgttgtgaa tgagcttaat acaataacaa gtattgttaa agcaatgacg
     4561 ttataccaag ttacagtcaa atttttcaaa tcctcatatg attgttttac taattctcta
     4621 aatttcatga ttcaatctct ccttttctta aatcaattaa tagttatact gcattgctta
     4681 aaatcaaaat atctaatgaa gcatctttta atcgtaagtg ttaaatggtt ttcttaatca
     4741 atttgtttga atgtacaaaa gcaattaaag caaaagtaat gtaaatgagg gtgtatacaa
     4801 caattactac aataaccggc atgaaggaaa tattacccat caatttcata aatgctattg
     4861 cggcgaatac tgcatgtaat atagcaatca ataatggtaa accgaagttg aaagtaattt
     4921 ttaataataa tcccttaagc atatctgtat gcgtaaagcc tatgcgtttt aatattctga
     4981 agttacttag ttcatcctca gtttcatcca tttgtttaat ataaataata catccagctg
     5041 ctactaaaaa tgctaatcct aaaaatgatg taacaaatat tagaataccg ttagtagcat
     5101 cgacctcttt tttcatttca tcatacgtga tgactttgtc tccaaactgt tttgcaattg
     5161 cttgagcttt ttccttttgt gatgtttgtt taatatcata tccataaaaa gtatgaacgt
     5221 tattttgtgt tttcaactgc tgatactttt caggacttac ttcgatgaca ggtgagttga
     5281 agcttagatt taaaggataa accttacctt tgtcttcttg tgtaacacgg aaagtttcat
     5341 tcttagtacc ttttactact aaatctttgt ttaaatggat attaataatg ttaggcagcg
     5401 attttgtatt tgtaatgatg gcattgttgc ctgttaactt gttatttgca cttaaaatag
     5461 aattcgtgcg acctgaatca ctaccatttt ccaaagtaat aacctgatca ttaacattat
     5521 ctacagtaat agtttcgtaa gcatttttag aaaatgtaat ttgttgttgg cttagtttag
     5581 tttcaaattg tttagcatct tgagtagcga ccacgttaaa ttcatttggt gccatagatg
     5641 taagggtttg atctgtattt gatttagata atgccgcaaa acacaataca gttactgtaa
     5701 ctgcagaaat aattgcaatg atagttaaag acatggcatt tttcttcatt ctgtacataa
     5761 tagacgatgt gaatacaaca tcggtaatag atacgcgtcc attttttgat tttttcaatg
     5821 ttttaaaaat aagtgacacg gaacttctga agaataaata ggcgcctaca accgttaaaa
     5881 ataaaatgat aaacggtgat gtcatagcca tagttagtgc tttgaacgta ccaaacattt
     5941 ctgtcgccat ataatagcct agtgcaatca tagcaatacc taatacgcct gaaataacct
     6001 ctgcagtcgt tactttagca gttgtggcat cagttttaat tgaatctttc atcattgata
     6061 agatactacg tctttttaga aataaagcac ttttaaataa aatcagtaca tacgcaataa
     6121 ttagcatgaa aatagttaaa acaagggcca taggttcgaa atgtatcgat aagttaatcg
     6181 ataacgacat caatttagat actatggaaa gcaataattg tgcacctgca atgccacata
     6241 atacaccgac aacacctgtg attaaaaata cgatcatttg ttcaagtgct aacattttca
     6301 aaatgttttg tcgtgttaaa ccaatcaact gaaatagcgc aaattcacgt gtacggcgtt
     6361 ttacgaataa atgattggca tacattaaaa agatgacaat aatgataaat aaaaatattg
     6421 atccgactaa agcacctttc ttaatgatgg ccatcgagtc gtcattattt acacctttag
     6481 taaactgtaa ggttgtaaaa ctgaaatata agacgatgct aaaaaataat gaaaataaat
     6541 acattgcata atgttttaag ttttgtcgta agtttttgaa aacgatatgg ttaaatgtca
     6601 tttgagacac cacctaatac tgattgaaga tgtacaatgt cttcataaaa ggcctgttta
     6661 gaacgtcctt cctgataaag ttgtgtatga atttggccat cttttagcat gatgacacgt
     6721 tctgcaaaac ttgctgcaac cggatcatgt gtaaccatga caatagttgt atcaaacgat
     6781 ttattcattt cttccaaacg ttgtaatagg tcatttgcac ttttcgagtc gagtgcgcct
     6841 gttggctcat ctgcaaatat gatttgtggt ttgtgaacaa atgctctcgc tgctgcagtt
     6901 ctttgttgtt gaccaccaga taattcgcta gggtatttat ttcctaggtc ataaatacct
     6961 aatgctgtcg tgatcgcttt ataattttct tccattgttg ccttcgacat tttttgaaca
     7021 gataaaggta acataatgtt ttctttaacg gttaatgtcg gcagaatact gtaatcttgg
     7081 aagatgaaac ctaatgattc tttgcggaat ttggcaagtg ctttttgatt aagtttatta
     7141 agctcttgtc cgttagcaat cacgctaccg ctagaaattt ggtcaattga acttagtaca
     7201 tttaataagg ttgtcttacc tgatccagaa ggccccataa tcgcaacgaa ttcgcctttt
     7261 tgtatgtcaa agttaatatc tttaagtgct tgaaatgtgt gctttttacc gtatgttttt
     7321 gaaacatgtt gcactgataa tatcgtcata gtctcactcc ttttgtattt aatttcattt
     7381 taaataatgt ttggagtagt agcctttatc taaacttaca attcaatgaa tgaaccttac
     7441 agagttgaaa gctatcgcta cttagtagat ttttgagtga ggatacagat tcatcgtaca
     7501 tattagacaa aagcaatggt gctttctaag tgatgatgtt tgtgtaaatt gagaaaaggg
     7561 aatttaatta ttgtataata aattttttgt aaaaattaaa agagggtttt atttgaaagg
     7621 aattgattgt tatggaaaaa ggaaatcaag gtattaaatg gtctagttta ataatgggtg
     7681 tattattatt aatgttggca gtcgttattt ttacatttcc aattgaaaat ttttatgcta
     7741 ttacctggtt gattggactg tttgtattaa ttaacggtgt gattcaaatc gtttaccgta
     7801 gaaaagcaaa agctttagta ggtggtaacc aaaattggat tctgtttatg gggattgtag
     7861 atattctatt tggtctatta gttattttta atgttggcgc aagttcagca ttctttattt
     7921 atatgtttgc tttttggttt atttttagtt ctatctctgg attatttacg ttttcgggta
     7981 gtggtagctt aaaactaatt tcagtgattt ttaatttatt aggtattgtt ttcggtgtca
     8041 ttttattatt taatccatta atgggtatcg tctttatttc gacgatgatt gctattgcat
     8101 ttgtattcgt aggtgtcatt tatgttgtag atgcacttgc ttaagtaaaa tgaagcggtt
     8161 caaaagaagg gtgtgacatg aagtttgtgt catatccttt ttgttgtgtt tatgaagcat
     8221 aaaaaagggg cgctacctac aataagtaag atacgcccat atttttatat tttactatta
     8281 ttgtttttca atacgattaa tagttacatt tagtccaaaa tatttttcta aaaaatgttt
     8341 atagttatct ttagtgacat caaatttttc tgagctacca ttccttgtta aagttaaatg
     8401 attttcagac attgtagcac ggccaaatga ttgtggcatt gtaattaata aatgctgtac
     8461 aaatattgaa tctggatgcg tttgattata ttcgatgttg ctattaaaat cttctatttg
     8521 tttaggtttg aattcagctt catattttgt atgccaatga tcattttcga atttttgaac
     8581 atagaaaata tccttgtctt cgttgttaaa aatagcgcgg aatgtaccac tgatatcagt
     8641 aattggttgt gtatgctcag atgaagtaat aggaatggca tgtagaggta agtctccaaa
     8701 gccaacatca gttacataga atacatcatt tatagaaaca acaagtgaag catgtgaacc
     8761 gttcagacta cgaccgccac cgggtgtgtg aatagtagct gacattaatt caggattaaa
     8821 tcctttttgt tgtaaatagg ctttgaaaaa tgtatttaat tcataacaaa aaccaccacg
     8881 tttatcatga acaattttat taaaaagtgc atcgatattt atagatatcg gcttactatt
     8941 ttgaacatca atattttcaa aaggtacagt taacataaaa cgtgttgcat aataatttaa
     9001 tgcttcaata ctcggtcgat tataacgaga tgaatcaatt tgtaaataat tctctaactt
     9061 cgcaatattc ataagcatag cgcctcctgt attaaagatt ataattaaat tttaaacaga
     9121 aatactgaaa ttttaaattc gaaagcattg aattttggat aaatacattt taaatagaaa
     9181 aatacgctct caaaatgaag tcatctctaa aagaaacgat ttaaagatga ctactgagag
     9241 cgtagcataa tggaagaagt gtgcagggtg tctaaaaatg caacaataca aaggtagttg
     9301 caagacaagt tgccttatct agaccatttg tgttctatgc gaccaaactt ccaaattaaa
     9361 cttgaaataa gccaagtaat taaaaataat gcaactaaaa tatagcctaa ataatcaaat
     9421 tcgatcgaac caatgaatgc ccaaaacgca ccatgtaaat ctaacttatc agcaagaatt
     9481 tgtagcaatt caatcatccc aatcactaat gctgccatga ctgatatcgc agtaatcgtt
     9541 atattgtaat agattttgcg aataggattg aagaatgccc aattataggc atacttcatt
     9601 acaacaccat ctaatgtatc caataaactc atacctgatg cgaataaaat tggtaaagat
     9661 aagattccga taaatgaaat ggcttgttgt gatgcgcctg aagaaagagc gagtaacgca
     9721 atttcactag ctgtatcaaa accaagtcca aataaaaagc caagtggcaa tacgtgccaa
     9781 ctacgcgtga ttaatttgaa ataaggtcct acaaatcgag aaaccaatcc tctagattca
     9841 agtaatgcat cgacttcagc ttcttcaatg tgttcacgac gtaatttagc gaacaagtta
     9901 attaaagaga ttaaaataat tagattcaac acaccgataa gcactaaaaa gaaacctgaa
     9961 actagtgtac caatcgttcc accaatatct tggaaatgcg gtaattcatc tttagcccat
    10021 tttacagata cccctaaaaa aacagccatt aaaaatacga cagatgaatg tccaattgaa
    10081 aaatagaaac ccacaccaga tggatctttg cgttgctgta ataatttgcg aaccgtatta
    10141 tctattgcag caatgtgatc tgcatcaaat gcatgacgca aacctaatgt atatgcaaga
    10201 atccccatac caaataagat atgatggtct tttccagcaa tccataaaaa actaaaccca
    10261 ataacgtgta acaaaatgac aatagctatg tatggtaacc agcttaagcg ctcattttta
    10321 aaaacagtca aaatatatcc ttctttcata acatgatatt taatcatact gtatgttcaa
    10381 tgggcactct agtaataagt gttcatataa caaaaatgtt atgccaaatt atttgttata
    10441 taaaaatata catgtaacca caaaagattt tttgcgatat atataatttg ataaattaac
    10501 caacaacaat gtaagatgtc actttgctta acttggcatc ctttttatga ttttcaaatt
    10561 caaaaaaatg agcaaaatga atctctttac cagtttttaa tatttcaata ccatgcatgg
    10621 aacctaagca cccatgtgtg atgctggaat ggatattgag actagcaacc tgattgtaat
    10681 gattagatag ttcttgaatt aatatttgag gtccgtatat gtcaaagcgg ccagggacag
    10741 accaaataaa ttctgttgta accagtgaac gtaataattc aatatctaat gctgctgtaa
    10801 caactataaa atctatcatt tgttgacgtt taggcgcatg attgcatgac acatctcctg
    10861 ttaacttaaa aggtaatgat gactgaactt ccgttttaaa atgtagttgg tgctgaaata
    10921 aagcttgttc caatattgat atagctttag gaccaacgcc atgcatctcc attagagatg
    10981 acttcgtata ttgtgatact gcttctaatg tgtatatacc ttgtgaattt agcgcgcgtg
    11041 ttgcaggctt tccaatttta ggtaaagtca tttgtatcca tcctttatgt tgtatggtag
    11101 tctttctggc aatataccct gatttgtaca gaattattta taaaattaat attgtttagt
    11161 ggttggtgaa taaatgtggg aaatataaac aaaatgatat ctttatcgaa atattattag
    11221 taaaatcgtt atattagcat aaccaagtga gcgtatgcag taaattaaaa attaggaaag
    11281 taaggaggtt tgaaggtgaa ttcagagtat aaaaaaggca tatttttagc actcagtgca
    11341 tacattctgt ggggaatact acctatatat tggcagttcg ttgatgcaat aggcgcattt
    11401 gaaattttag cctttcgtat tatattttca gcaatattca tgattttcat actcgcggtt
    11461 ggacaaaaac aacgcaatgc atttcaacga gatatgaatc aattgttagg caagcccatt
    11521 cagctattag cgattgtcgt agcaggctat gtcattacat taaattgggg tacatttatt
    11581 tgggctgtaa cgaacggtca cgtcctacaa acaagtttag gttattatat aaatccactt
    11641 gttagcattt tgctcgcact tatcttttta aaagaaagat tcaataaatt tgaatggcta
    11701 gccattttat tcgcattcat cggtgtatta tatatgacgc tcaagattgg agaattccca
    11761 atcgtctcta ttatattagc gttatccttt ggtacatacg gattattgaa aaaagtagta
    11821 catattgatg ccatcagcag tattacgatt gaatgtattg ttaccgcacc tgctggacta
    11881 atatacgtta tttatttatg gcagcaacat cagatgtcat ttggattgaa catgtcatca
    11941 ttttggttgt tattttctgg tgctattacg gcaataccac taatcctatt ctcagccggg
    12001 gcaaaacgta ttccactttc gctaatagga tttattcaat acgttggacc aacaataatg
    12061 tttgtactcg gcatatttgt tttcaaagag ccttttagta tagatcaatt aattacgttt
    12121 atatttattt ggacaggtat tgtgttatat agtctttctc aatacattaa attgaagaaa
    12181 catccggtcg caaaaaccct ataaaatacg aggaaagata attatacgtg ttttcaatat
    12241 cctttgtgtc atgatgaggt gagactgagg tatgtgtgca gtaacagtga cagacgtata
    12301 aatttgaaac attaaatatg aaataaggca ctttcgttga attcgcaatt aaaatgaatt
    12361 taaacgaagg tgttttttgt atgatgattt atgagggcat acgtaatgtt aatcaatgaa
    12421 gcggaatagt atagaaatgt attttatgtg atgttacgta atgacttaaa tctttataca
    12481 aattttcagt gaagcacgca atgatttaaa ggataaacgt attttaaaaa gggagtcttg
    12541 atttagcaaa ggttgatttt aaatacaaat taaaaagcgc ccaaatcaat gagattcgag
    12601 cgctaattta aatattttaa aatataccaa ttactttcat ccaaagtgat cctaaaccaa
    12661 tccagataat aaagtagacg aaacctaata ttaagttcat tgtccaccaa cgtttttgag
    12721 tcacgtaacc tgaagagaat agaatcggcg ctggaccact actatagtgt gttgttgaag
    12781 ctaataggtt accgaagaaa cctaacatta atgcactgaa taatggtggt gcaccggctg
    12841 cgatggcaac gcctagtaat gctgcataca tcgcactgat atgtgctgta gaacttgcaa
    12901 ataagtaatg tgaatagaag tagaacaata ttaaaatgac caggactata ggccagctta
    12961 agccaccaag acttgtagca atggatttac ttaaccaagg aataaatcca agcttgttta
    13021 attggtcggc cattaacact aatactgaga accatactaa tgtgttccaa gcacctgttt
    13081 cgtttaaaat gtcttgccat gttaagacgc ctgtcaataa taacaatgct agcgcaataa
    13141 aggccgttaa agttgcatca atatgaatga aacttccgac aatccatagt gttaacgcaa
    13201 cgacaaaaat accaatcata aatttttcag ctaaagcgat tttacccata gtcgctaatt
    13261 cattttcagc ccaactctta gcatttggtg tttctttaac agttggtgga taaattttat
    13321 aaataataaa aggtacaaca attaaggaaa caagtccagg aactaaagca gctagaaacc
    13381 aattcatcca tgtaatgtga acattagatg tgctagatgc taaattttgt gcaagggggt
    13441 taccggccat tgcagttaaa aacatagccg cagtaattaa attaccttgg aattctgtga
    13501 aaacaagaaa tgcacccatt ttgcgtgctg atccgtcttt cggtttcgaa ccaaatgatt
    13561 cagaaagtga tttgataatt gggaacatga ttccaccagc acgcgcggta ttacttggtg
    13621 tagcaggcgc tagaattaaa tctacaccga cgatagaata tgctaatcct aatgtttttt
    13681 taccaaataa tttgacgaaa tgaagtgcga tacgtctacc aagacctgtt ttcacaaatc
    13741 ctctcgaaat gaaaaatgcc atagcaatta accaaatgct attattacca aaaccagcga
    13801 cagccgtttt catgtcaaca atgccaacga gtaccatgat tgtaaatcca attatagaga
    13861 cggccccaat tggcatcggt tgtgtaatac aagcaatgat tgtcgcgacg aatattgcga
    13921 acatatacca tgctgttgga tccacagcat ccggtttaaa aggtgtaagt gcccaaataa
    13981 ggagacctac aacgataggg agtataaact tacgatattt aaccgtgttt tccatgttaa
    14041 aacgtccttc tttctatgtt ttatacatat ttcaatttaa gaataaagct aactacaaaa
    14101 gatgtacagt aataattaaa tataaaatta aattaacgaa atcattaata taattatttt
    14161 tcgagaatcg gtgaagaact ggtatagatg gtgtttatta aattcaaaaa agtgaccttt
    14221 taaacaaata aaaaacccgt taacgtgttg ttaacggtta ataagcacat atttatttta
    14281 aattttttaa tatacgagac atctcttcaa ttttaccagc atgaagtggg atgtgaatgg
    14341 catttaatac taataactct tctaagttat tacaaccgac aattggctgt ttcaattcaa
    14401 ttgctaaatc atcttcagtt agatttcgtg cacgttcagg taaagtttgg agaccttcta
    14461 aaatcctttc aatactcggt acttcatcct tccattcatc tggagacgaa ccatttccga
    14521 ataaaggtct atagatattt aaatcaatat tctctttacc agcaacagct aaggccgatt
    14581 caaaaattgt taatacatga ccaaattgcc aatgaatggt attagaaaaa ggcttatgat
    14641 aatcatcaag taccttttca actttccaag catcatacac atttactaaa tatcctaacc
    14701 ccatatcaat gacatcaaat actgtttttg tagtcattat taattcctcc cccataaata
    14761 aaatatactt ccgtaagtat taccctatta acaaagttgt tagtcattta aggaatgtgt
    14821 acaacaacta ccttacaata aaagatagtt gacgaaaagt tctcaatgta ctatcttatt
    14881 atgtaagata gtaggtgatt aaaatgtcag tgtcagatca gattatgaaa ggtctcttag
    14941 atggggcgat attaggtctc attgcccaag gcgaaacgta tggatatgaa attatgacga
    15001 aacttaagaa tcaagagttt ccagagataa gtgagggtag tatttatcca gttttaatgc
    15061 gtttaaacaa aaaaggatac gttgatacga caataaaaaa atctagtaat ggcggcccga
    15121 ggcggaaata ctatacgatt accgattcag gtttaaagga actaaaagta tttaaatcga
    15181 aatggcaagt tttgaataaa gggatgatta acttgtttgg aggggaaaac gatgttaagt
    15241 aaacaatctc aagatttttt agaaaaatta cgtgttgaat tgttatttag aggtaagact
    15301 gaagcagaag tagaagagtt attggaagag ctcgaagatc atttaactat ggctgaaaaa
    15361 aataacgaag atacaagttc aattattaat acaccgatta agtcaatggc tgatcaatta
    15421 tcacctgaga taagtcttac taccggtttg tataaatata ttactttgta cttcgctttt
    15481 attttagcag tcattataat tcctaggttt ttagatttgg gtacatttga tgtgacaatt
    15541 gcatttttac tttatatagc tagtatcgtt attttaggtg ttgtcattgg tatgctgatt
    15601 ctacgaaata cattagtccg atttggtgac caaaaaataa cgtatgttat aaatgtgact
    15661 tatggaatta tcgtattttt atggatgatt ttaggtggct tgattattaa aaaatatcct
    15721 atctatagtt tctttgagtt aagtgacaaa cagagtttca tcgttgggtt agtactttta
    15781 attgcgctca ttggaacttg tattttaatt aaacagaaaa tttatgcgct catcgtgttg
    15841 gtaatttcat tgccaagttt gatagcaagg atagcagcta tctttagtaa agaaccagac
    15901 acttttaatt tagtttcagt aagtatactc atcattctaa atatagcatt tattatttat
    15961 acatttgtaa attttaggaa gttaaaaaaa gaagactaaa caatgtcgtt tagctaaaat
    16021 acatcattat agtaaaactg tataccaatc atccaaaagc accctaaagt tagacttaaa
    16081 ctaacaaatt agggtgcttt tgtttgtgca cttataacta ttccgttgtg cccagggcta
    16141 ggtgtaaatc ctcatcatta gaaatagctt caatggctgc agtcaaacct gcaactatct
    16201 tttctaatgg catagatggt gtatcgggtt taccaatgac ctgctctggt atgtatggca
    16261 catgaataaa tccgaatcgt aggtgagggt aatgcttatc ttgtaaataa cctaagtgat
    16321 aaagtgtgtg attacaaaca aatgtacctg cgctatttga aagtgctcca ggaagtcctt
    16381 gattaataat actttgagtc attgctttaa ctggtaaatt tgaaaaataa gctggcgcac
    16441 cgtctaagtg aatggcttga tcaataggtt gaaaatcatc attatctgga atacgtgcat
    16501 catcaatatt aatggcgaca cgttctgggg taatggcatt tctaccacca gcttgtccta
    16561 tagctagtac aacatcataa tgattagatg ccaacgtttt atttataata ttatctactt
    16621 tcttaaaaga ggttggtagt tttaatttat cgattgtatg tgtgccaata atatcttcta
    16681 gttgagtcac agcttcccat gagggattga tattttgatt gtcaaaaggc gcaaaccctg
    16741 ttactaaaat gtgcatttta agacaccccg cagtattaaa atttaattta gtataacacg
    16801 cgcataatta aaaacatatc aggttatgta tgcgcataaa aaacagcctt gaaaaacctt
    16861 tataaatcgg attttcaaag cgtgttttga aaggtattta attaaaacta agcatgttga
    16921 tgtaaatcat caaagtttgt taaacgttgt tgccactcat tatcactaat attatttgct
    16981 tgaacataac gattacgctc atgtttagca cattcataag agcatgcacc taaatattta
    17041 gtttcgtttt cttcagaaac taatatttgt ttattacatt ctgggttagc gcaattaata
    17101 taacgttcac atggtttgcc atcaaaccaa tccttaccaa taattgtttt ttcaacttgg
    17161 ttgatatcaa cactgatacg gtcatcaaat acgtacattt taccgtccca atattcacct
    17221 tttgtttcag gatctttacc atatgtagca ataccgccat gaagttgagc tacatcttcg
    17281 aaaccttctt ttaaaagcca tccagaaaat ttttcgcatc gaatgccacc agtacagtac
    17341 gtaaccactt ttttatctgc aaataacgct ttattctctt taatccagtc aggcaaatct
    17401 ctaaaacgtg tgatatttgg acgaattgca cctcggaaat gacctaaatc aaattcataa
    17461 tcattacgtg catcaataat gactgtgtca tcatcttcaa gagcttttct aaattctaca
    17521 ggtgataaat attggccagt tgtgtgtctt ggatcgacgt catcttctaa atctaaagca
    17581 acgatttctt ttcgaggacg tacatgcatt ttcttaaaag catgtccttc agcttcatca
    17641 attttaaaca ccatatcttt gaatcgttca tcggcatgca tatgtgccat atattgttcg
    17701 gtttcttctt ttgtaccaga taatgtgccg ttaatacctt ctgtagaaac aagaattcta
    17761 ccttttaaat ggtgtgcttt acaaaaggct aagtgatcct gagcaaactg ttcagggtca
    17821 tcaatcgtca tatatttata atataaaaga acttgatagt tcataggtta atctccttct
    17881 ataaacatgt tataagcatg ttaattttta aaataaagcg tcaatacgca tatttgtcta
    17941 cgtccatcat tttaacagag ttgtattgtc attcaatatt tctagcttga aatcagccac
    18001 tagaccgata aaatagatag atgaaaccgt tatcatttta tttgatagtt atcttgttta
    18061 aaataaaatt gatagttaaa acgaatgaaa tgaaaggaat gatcatgatg tcacaacaag
    18121 atttacctac attattttat agcgggaagt ccaatagtgc tgttccaatt atatctgaaa
    18181 gtgaattaca aacaattaca gctgaaccat ggcttgaaat ttccaaaaaa ggattgcaac
    18241 tagaaggatt gaactttgat cggcagggac aactcttttt attggatgta ttcgaaggca
    18301 atattttcaa aatcaatcct gaaacgaagg aaatcaaacg accttttgta agtcacaaag
    18361 cgaatcctgc agcaatcaaa atacataaag atggccgatt attcgtttgt tatttaggag
    18421 attttaaatc tacaggaggc atttttgcag ctacagaaaa tggtgacaac ttacaagata
    18481 ttattgaaga tctttcaaca gcatattgta ttgatgacat ggtatttgat tctaaaggtg
    18541 gattttattt tacagatttt agaggatact ctaccaatcc actaggaggc gtttattatg
    18601 tttcgccgga ctttagaaca gtgacgccta tcattcaaaa tattagcgta gcaaatggta
    18661 ttgctttaag tacagatgaa aaagtactat gggtaacaga aacaacagcc aatcgattac
    18721 atcgcattgc acttgaagat gatggtgtga cgatacaacc atttggagct accataccgt
    18781 actattttac aggtcatgaa ggaccagact catgttgtat tgatagtgac gataatttat
    18841 acgtagcaat gtatggtcaa ggtcgagtgt tagtttttaa taaaaggggt tatccaatag
    18901 gacaaatatt gataccaggc cgagatgaag ggcatatgtt acgttctact catccgcaat
    18961 ttatacctgg aacaaatcaa ctcatcattt gttccaatga tatagaaatg ggcggaggat
    19021 ctatgcttta tacagttaat ggctttgcaa aaggtcatca aagttttcag tttcaataag
    19081 acctacctga atgaaaggcg tctaaaatta gggtgaacag ttagtgcttg ttacacatga
    19141 gttaaacgaa aaatgccaac tatacctagg gatgatatag ttggcatttc gtatatatat
    19201 taaaacaatg aggattgtgt aatgagatta ttctgagatt gagaattcag ctgatgcttc
    19261 gaatttaaca tctttgccta gcattacgcc accagtttca agtgcttggt taaagttaat
    19321 gccatatttt tctctattga ttgtaccagt aacaataata cctgttactt gagaaccatc
    19381 cataggattc ttacttactc cgttgaattc aacatcgaat gtttcttcgt tagtgatgcc
    19441 tttaattgtt aaatcaccaa caactttgct ttcagatact gattttgtca caaatgtaat
    19501 tttatcaaat tcgtcagtac caaagaaatc accagatttt aagtggttat cacgtgcttc
    19561 gtttttagtg ttaattgagc ttggaataat tgttgcagta gctttcaaag tactgaagtc
    19621 attaatatct ccttcaacag ctacatcaaa ttgatcaaat gaacctttca ctttagaaac
    19681 cattaaatgt ttaatttgga attctaaact actgtgtgca ccatcaaaag taaagttagt
    19741 cataatataa aacctccata tttttatctc ttagatatat tttttatact tgatatacat
    19801 acaataccac ctaaaaaaca aagatgcaag cattttattt cgaattcgaa tattttctgt
    19861 gatgtattta aagaatgacc tataaaacac cacactattg ttatacccta gtcaattaat
    19921 tcaaacataa agaagtgatt acaagtccgt gaaatttagt taatgagata aatctgacta
    19981 ttattatcat ttttatagtc actactattg actttgcatc aagtgactcg tactatttta
    20041 atataattct gaaaattcaa aatatagaac attaaatatg tagataatat ttttttgaag
    20101 ttttaaaaaa aggaggcatt ttatgggaaa ttatttggta ttgcaatcag attttggact
    20161 tagtgatggc gctgtaagtg caatgtatgg agttgcatat actgtaagtg atgatatacg
    20221 tgttgaaaac ttaacgcatg atataccacc gtatgacatt tgggtagcgt catatcgttt
    20281 atatcaaact gtaaaatact ggcctaaagg cacagttttt gtatcagtgg tagacccggg
    20341 tgtaggtagt gataggcgga gcattgcttg cttaacatat tcaggtcatt acattattac
    20401 acctgataat ggttctttaa gtcatattaa acattacgaa ggtattaaaa aagttatcga
    20461 aattgatgaa gtgaaaagcc gattgcctca ttcggaagaa agtcatactt ttcatggtag
    20521 agatgtttat gcatacaatg gtgcgagatt agctgcaaat aagatagcgt ttgaacgtct
    20581 cggtcaggca attaatgttg atagtattga ggcattagaa ataagagaag tgacaaaaaa
    20641 tgaggattct gttacaggaa gcattgatat tttagatatc agatttgggt cattatggac
    20701 aaatattcca ttggcattct taaaatcctt ggaaattgtt catggtaata atttggtggt
    20761 aacaatttat catcaagata agaaagtcta tcaaaatatt attaaatttg cgcgatcgtt
    20821 tgcagatgtt aatgtgggtg aacctcttgt gtatgttaac tcgctagtaa atattggtgt
    20881 agcagtcaat caagactcat tttcagattt atatcatatt ggtactggaa atgattggac
    20941 gattcattta tctaaagcac catcaatata atgaattttt tatgtaagga gtaatgaaga
    21001 tgaaaaaaca agatatttca gttaaaactg ttgttgcaat tggtatagga gcagcggtat
    21061 ttgtaatttt agggcgtttt gttgtaatac caacaggttt tcctaatacg aatatagaaa
    21121 catcatatgc atttttagca ttaatatctg caatatttgg accttttgct ggtttaatga
    21181 ctggattagt tggacatgct attaaagatt ttacgacata cggtagtgcc tggtggagtt
    21241 gggttatttg ctcgggaatt ataggctgtt tatatggctg gattggacta aaattaaatc
    21301 tttcttcagg ccgattttct aggaaatcga tggtttattt taatatcggt caaattattg
    21361 cgaatattat ttgctgggca cttattgcac caacattaga tattttgatt tataacgaac
    21421 cagctaacaa ggtttataca caaggtgtta tctctgcagt attaaatatt atttcagttg
    21481 gcattattgg gacaatatta ttaaaagcat atgcttcatc tcaaattaaa aaaggtagtt
    21541 tacgtaagga atagttattt tgttgaatca gatatgtaaa tgaatataga aaggtaatga
    21601 tatatcatga ctgaaccaat tatctcgttc aaagacttta gttttcaata tcatagtcaa
    21661 gcaacaccta cattacagaa tataaatgtt gatatttatc caggagaaaa agtattagta
    21721 gttggtgctt cgggtagtgg taaatcgact tttgcaaatt gcataaacgg attaattcca
    21781 tttaaaacta aaggtaacat aactggggaa ctatatataa ataatcaaga tgcaaccgtt
    21841 agttgtttac atgatagatc taatgttgtt ggtacagttt taaaagatac agatggacag
    21901 ttcataggct taacagcagc tgaagatatg gcctttttat tagaaaataa ttgtgttgaa
    21961 caagatgata tgaagaaaaa tgtaagttat tgggctgaaa aagttggcat gatagaacat
    22021 ttaaatcacc gaccgcaaga tttatctgga ggtcaaaaac aacgcgtttc attaggtggt
    22081 atattaatcc atcgtacgcc tattttaata ttggatgagc cactggccaa tttagatcct
    22141 gcgacaggac atgaaacgct gagattgtta aacaatattc atgaagaaac aaagtcaacg
    22201 atgattattg tcgaacatcg attagaagaa tcattagatg atacgtttga tagagttctt
    22261 ctttttaaag atggaaaaat catcgctaat acaacgccta gtgatttgct aaaatcatct
    22321 aagcttaaag aagcaggcat acgtgaacca ttatactgta cagcgcttaa atatgcagaa
    22381 gtagatgtcg aatccattga taacttagcg aatttacgtg acgtttgtat gagtgaacat
    22441 gttaaattta aagtgaaaaa atggatagat gaaacatctg caaataatga taataagtac
    22501 aaatctgaac cgctattaga attaaatgag gtatgtgttc aatatagtga ctatagtaat
    22561 agtgtactta ataatgttca attaaatgtt tatagaagag aaatgcttag tatagttggt
    22621 cacaatggtg ccggcaaatc aacacttgct aaagcaattt gtggattttt agatataaca
    22681 ggtaatatcc aattttgtaa taggggattt aatcaattgt caattagcga acgatctgaa
    22741 ttcgtaggtt atgtgatgca aaatccaaat catatgattt ctgaaaaaat gatttacgat
    22801 gaagtagcat tagggttaag agcacgaggt atgaaagaat cagatataaa gatacgagta
    22861 gagaatgtgc tcaaaatatg cggactttat gcatttcgga attggccaat tgcagcttta
    22921 agctatggtc aaaaaaagag ggtcactata gcatctgttt tagtcttaaa tccggaaata
    22981 atcatattgg atgaaccgac tgctggtcaa gatttctatc attataatga gataatgtca
    23041 tttttaattg aactaaacag acaggggaag acgattatta tgattacgca tgatatgcat
    23101 ttattgtctg agtatagttc aagaacagtt gtattatcaa aaggtcaagt cgttgctgat
    23161 accacgccag tattggtttt aaatgataaa aaaatctgtg agattgcatc attgagacaa
    23221 acatcgctat ttgaaatggc cgaatatata gggattagcg agccacagaa attagtacaa
    23281 ttatttatta accatgatag gaaggtgaga cgccaatgaa tcaatataat actataggtt
    23341 ttcacccggg aaatagtcgt attcatcaat taaatgcgac tgttaaactt ttattcttat
    23401 tagttgtttc tatttctgca atggtgactt atgacacaag atatttaatt ttaattagtg
    23461 cttcatctat tttattggtc aaatatgctc atattgaatg gaaacaagtt cgctttgttg
    23521 ttaaattcat tctgtttttc acaatattaa atattattgc cgtgtacata tttgaccctg
    23581 aatatggtgt gaagatttat aatcagcgta cagagttagt caatggtatt ggtcgattta
    23641 cgctaacatc acaggaatta ttctatcttt ttaatctaat attaaaatat attagtacag
    23701 ttcctttagc gttaatattt ttattcacaa cgaatccgag tcattttgct gcaagtttaa
    23761 atcagctagg tgtgaattat aaaatcagtt acgcagtctc actagcatta aggtatattc
    23821 cagatattca agaaacatat tttaatattt cacaagcgca acaagcaaga ggatatgata
    23881 attcgaaaaa agcaaaattt actagccgtg ttaaaggtat caaacgcatt gtgttacctt
    23941 taatattttc tagtatcgaa agaattgaca ctattagtac tgctatggag ttaagacaat
    24001 tcggacagta taaaaggaga acctggtacg tcaaaaaaca attaaaaaaa gatgattatg
    24061 ttgttttgtg tttgacgtta atacttctga tgttagtagt tacattattc tttttaaata
    24121 atagtcgata tttcaacccg tggcattagt attcatataa atagtcttta aatagaaata
    24181 ggagggagac atttaatgat aaatactgaa agattaaatt taatgattcc aagttcctcg
    24241 catttaattg aactttataa tatttgtagt catccacaag caaatatata cactcccaaa
    24301 ggtttacata attccaaatt agacacacaa cggtggattg aaaaatggcg aaaccattgg
    24361 caacaatatc aatttggtta ctttgtattg gtaaaaaaaa tagattgtag tgttattggt
    24421 atttgtggat atgaatatcg acaattaaag caagaaacag tattaaattt attttataaa
    24481 ttacatccaa gttttgaagg acaagggtac gcatgtgagg ctattacagc aatcacaaat
    24541 tttgtgaatt atatcgatca agaaacagta aaagttatca ggacaaataa gtgtaaccaa
    24601 cgttcaataa atttagcaga aaggcttaaa ttcaagcgag acgatactat ggacgacatt
    24661 atcaatcaag gagatattgt gttttataaa taaaatacta tgacattatc taaaaaataa
    24721 aattaaaatc ccaacagttt cttaaaaagt tatgagccac ataataaaaa tgttataatt
    24781 gtgcataata aaacataggg agaatgttta atgcataaaa aatatttatt gctatttttt
    24841 ctgttattat cagttgcatt gttatatagc tgttccaagc aaaatgagaa agctcaaaat
    24901 aaatctgtta atccaaatga gtatgcaaag cataaatcga aaatggatga acattgggaa
    24961 acatatcacg gccaagttta tcatgtgttt tatcacccat taattacaga cccaaaagtt
    25021 gcgtttacag gagatacaca ccaagctaag ggcaacaatg attggatgat tactgtaagt
    25081 gaatttaaaa aatcattgaa cgaattatat aaaaataatt acatcatcgt taatccacat
    25141 gatgtatacg acttatcatc tcatcatgta aaagccaaaa aaataaaatt gccgaaaggt
    25201 aaaaagccac taatcatctc aattgatgat atgaattact atagttacat gagacaccat
    25261 ggttatgctg accgtttagt actcgataaa aataaacatg ttgtttctga aactacagac
    25321 aaaaaaggcc atgctactta ttctgatgac aatgatatcg tgccaatttt aaataaattt
    25381 gtaaaagagc atccagattt ttctttaaat ggtgaaaaag gtgttgttgc tttaaccggt
    25441 tatgagggcg tattaggtta tcgcacgaat gaattaacga gtaaagatta tacaaagaat
    25501 aaaaaggcag ctgaggaagt tgttcgcgca atgaaacggg atggatggtc atttgcgagt
    25561 cattcatatg gtcacattaa ttttgaaaaa acatctttag aaggtattaa acgtgatact
    25621 aaaagatgga aagatgaagt tgaaccaatt gtaggtaaaa cagatatgtt tgtatttcca
    25681 catggcgcac aggatagaca tacacaagcg tatgattatt tggtagatga agcagagttt
    25741 aagtttattg caggtgtcgg tccaaacaat tttacagata tatcagcaac gaacgtttac
    25801 caagataggg ttgcgattga tggtctaaac ttatttgaat ttaaatataa actcaaaccg
    25861 ttttttaatc ctgaaaatgt atatagtaaa caagatagac gttactttaa agggaatcgg
    25921 gattatgaag aatagttaaa tttaataaaa agatagaaag aaaataactt tacataataa
    25981 ggcaaggaca atatgtgcaa gaagtacaac ttcaatataa aaatcttggt taagcatttt
    26041 tataggcaca aatgtcttgc cttttttatt ttaaaaaatt ttaaaatcaa tacaccgaat
    26101 tttctgaaaa aggttgtaat gtattgctaa aaatgataag ttattaatca tattacactt
    26161 tagcgtggta gagaagtaaa gcaaacaacg aaaataccaa gtgattttaa atacagtatt
    26221 gaaagtaaag ggtggtcgag tgaataattc agaacaatta attgcgttaa aagaatcgga
    26281 aacagcattt ttaaaatatt ttaataaagc ggattatgag ttagttgatt ttagtgtggt
    26341 tgaaaagttg gactggaaac aattgaacca tgaggatttg cagcagatgg gtgagcgaaa
    26401 tttttggcaa catgagcatc aaatttatgc acttcgaaat gattttacag atcaattatt
    26461 gcgttattac tcaatgtatc caactgcagc aacaaaagtg gcatacacag gtttaatcat
    26521 tcggaataat gaagcggctg tacaagttgg tttggaaaac tatgcaccat cattagcgaa
    26581 tgtgcaacag agcttaaagc tctttattca gtttattcaa cagcagttga gagacaacgt
    26641 gcattttgtt gtattgggac attatcaatt gcttgatgcc ttactcgata aatcgttaca
    26701 aacaccagac atactatcaa tgattgagga acgtaattta tcgggactgg ttacatattt
    26761 gtcgactgaa catccaatcg tgcaaatctt gaaagagaat acgcaacaac aattaaatgt
    26821 attggaacac tatattccaa atgaccatcc agcactcgtt gaattaaaaa tatgggaacg
    26881 ttggttacat aaacaaggtt acaaagacat ccatttagat attactgcgc agccacctag
    26941 atcctattac acaggtttat ttattcaatg tcattttgct gaaaatgaat ctcgagtttt
    27001 aacaggtggt tattacaaag gaagcatcga agggtttgga ttaggattaa cactttaagt
    27061 aagggagtat gcacaatgtt aagaatcgcc atagccaaag gacgtctaat ggatagttta
    27121 attaactatt tagatgtaat tgaatatacg acattatcag aaacattaaa aaatagagaa
    27181 cgccaattat tattaagtgt agataatatt gaatgcattt tagtaaaagg aagtgacgtg
    27241 ccaatctatg tggaacaagg aatggcagac ataggcattg ttggtagcga catattagat
    27301 gagcgccaat ataatgttaa taatttgttg aatatgcctt ttggagcatg tcattttgcg
    27361 gttgcagcga aacctgaaac gaccaattat cgtaaaatcg caacgagtta tgttcatact
    27421 gctgaaacat attttaaatc aaaaggtatt gatgtcgaat tgattaaatt gaatggctct
    27481 gttgaattgg ccggtgttgt agatatggta gacggaattg tcgacatcgt tcaaacaggt
    27541 actacgctaa aagcgaacgg actggttgaa aagcaacata ttagtgatat caatgcaaga
    27601 ttaataacta ataaagcagc ttattttaaa aaatcacaat taatagagca atttattcgc
    27661 tctttggagg tgtctattgc caatgcttaa tgcacaacaa tttttaaatc aattttcatt
    27721 agaagcacca ttagatgagt cattgtatcc aattattcgc gatatttgtc aggaagttaa
    27781 agttcatgga gataaggctt tgaaaatgta taatctaaca ttcgatcata cgaaaacaga
    27841 tcatttagaa attagtcatg aacaaattaa agcagcattt gacacattag atgaaaaaac
    27901 aaaacaagca ttacaacaaa gttatgaaag aattaaagca tatcaagaaa gtattaaaca
    27961 gacgaatcaa cagttagaag aatcagtgga gtgttatgaa atataccatc cactagaaag
    28021 tgtcggtatt tatgtgcctg gtggtaaagc aagttatcct tcaacggtat taatgacagc
    28081 gacgttagca caagtagcag gtgtggaaaa tattgtcgtt gtgacaccac ctcaacctaa
    28141 tggtgtgtct caagaagtat tagcagcatg ctatattacg caagttgatc aagtatttca
    28201 agttggtggt gctcaaagta ttgctgcgtt aacttatggc acagaaacga tacctaaagt
    28261 tgataagatt gtaggtccag gcaatcaatt tgttgcatat gccaagaaat atttatttgg
    28321 acaagtaggt attgaccaaa tagctggacc aacagaaata gcacttatta ttgatgaaac
    28381 agcagattta gatgccatcg tatatgatgt ttttgcacaa gcagaacatg atgaattagc
    28441 acgtacatat gccattagtg aagatgcgca agtccttaaa gatttagaat cacgtattgc
    28501 taaagcattg cctaatgtgg acagatacga cattgtttct aaaagtatcg ctaatcaaca
    28561 ctaccttatc catgctagta attttgatga agcatgccat gtcatgaata caatcgcgcc
    28621 tgaacatgcg tcgattcaaa cagtaaatcc tcaaccatat attgaaaaag tgaaatatgt
    28681 gggtgcattg tttattggac attattcgcc agaggtcata ggagattacg ttgcaggtcc
    28741 aagtcatgta ttacctacaa atagaacagc tagatttacc aatgggttat cggtcaatga
    28801 tttcttaaca cggaacacgg tcatccattt atcaaaagat acgtttgaac aaattgctga
    28861 ttcagcacaa catattgctc atgttgaagc attatacaat caccagcagt ctattttaat
    28921 acgtcagtct taggggagtg taattgaaat gatttatatt gataaaaatg aaagtccagt
    28981 tacgccgttg gatgaaaaaa caatgacgtc tattattagt gcaacgccat ataatttata
    29041 tcctgatgca gcatatgaac aattcaagga agcttatgct aagttttacg gattatcgcc
    29101 tgaacaaatt attgcaggaa atggatctga tgaattgatt caaaagttaa tgctgatcat
    29161 gccagaaggt ccggcattaa cgctaaatcc tgattttttt atgtatcaag catatgcggc
    29221 acaagtaaat cgtgaaattg catttgtaga tgcaggatca gatttaacgt ttgatttgga
    29281 aaccatttta acgaaaatcg atgaagtaca accatcattt tttattatga gtaatccaca
    29341 taacccttca ggcaagcaat ttgatacggc atttttaaca gctattgcag ataagatgaa
    29401 agcattaaac ggatactttg tcattgatga agcatattta gattatggta cggcatatga
    29461 cgtggaactg gcaccacaca tcttaagaat gcgtacatta tcaaaggcgt ttggaattgc
    29521 cggcttaaga ttaggtgtct taattagtac tgctggaacg ataaagcata ttcaaaaaat
    29581 agaacatcca tatccattaa atgtatttac gctaaatatt gcgacttata tttttagaca
    29641 tagagaagag acaagacaat ttttaacgat gcaacgacag ttagctgagc agttaaaaca
    29701 aatatttgat acacatgttg cagataaaat gtcagtgttc ccatcaaatg ctaattttgt
    29761 acttactaaa ggctcagcag cgcaacaatt aggacaatac gtatatgaac aaggatttaa
    29821 acctcgcttt tatgatgagc cggtgatgaa gggctatgta agatactcaa ttgcaacagc
    29881 atcacagtta aagcaattag aagaaattgt taaagaatgg agtgcaaaat atgatttatc
    29941 aaaaacaacg aaacacagct gaaacgcaac taaatatttc aatatctgat gatcagtcac
    30001 catcgcatat taatacaggt gtgggctttt taaatcatat gttaaccttg tttacatttc
    30061 atagcggtct gtcattaaac attgaggcac aaggtgatat tgacgtagat gatcaccacg
    30121 taactgaaga tatcggcatt gtcattggcc aattgttact tgaaatgatt aaagataaaa
    30181 agcatttcgt tcgttatgga acgatgtaca ttccaatgga tgaaacatta gcacgtgtcg
    30241 ttgtggatat aagtgggcgc ccatacctat cattcaatgc atcattaagt aaagaaaaag
    30301 ttggtacgtt tgatacggag ttagtagaag aattttttag agcggtcgta atcaatgcaa
    30361 gattaacaac gcatattgat ttaattcgtg gaggtaatac acaccatgaa attgaagcta
    30421 tattcaaagc gttttcccgt gcattaggca tagcgctaac tgcaactgat gatcagcgtg
    30481 tgccgtcatc gaaaggtgtg attgaatgat tgtcatcgtt gattatggat tagggaatat
    30541 tagtaatgta aaacgcgcta ttgaacattt agggtatgag gtggttgtct caaatacctc
    30601 aaaaataatc gatcaagcag aaacaatcat attgcccggt gtcggccatt ttaaagatgc
    30661 gatgtcagag ataaaacgat taaatctcaa tgcaatattg gctaagaata ctgataagaa
    30721 gatgattggt atttgtttag gcatgcaatt aatgtatgag catagtgatg aaggcgatgc
    30781 atctggatta gggtttatcc caggaaatat ttcgcgtatc caaacagaat acccagtgcc
    30841 acacttaggc tggaataatt tagtgagtaa gcaccctatg ttaaatcaag atgtttattt
    30901 cgtacattct tatcaagcgc cgatgtcaga aaatgtaatt gcatatgcgc agtatggggc
    30961 tgatattccg gcaattgttc aatttaacaa ttatattggt attcaattcc atcctgaaaa
    31021 aagcggtaca tatgggttac aaattttgcg tcaggcaata caagggggat ttataaatga
    31081 ttgaattatg gccagcgatt gatttgattg ggtcaacaag tgtgaggtta acagagggta
    31141 aatatgatag tgaagaaaaa atgtcacgct cggctgaaga aagtattgct tactatagtc
    31201 aatttgaatg tgtgaatcgt attcatatcg tcgacttgat aggtgctaag gcacagcatg
    31261 cccgagagtt tgattatatt aagtcattga ggagattaac aaccaaagat attgaagtag
    31321 gtggtggcat tcgtacgaag tcacaaatca tggactactt tgccgcaggg attaattatt
    31381 gcatagttgg aacgaaaggt attcaagata ctgattggct taaagagatg gcacatacat
    31441 ttccaggtcg catttattta tctgttgatg cctatggaga agatattaaa gtgaacggat
    31501 gggaagagga cacagagtta aatttattta gttttgtgag acggttatcg gatatacctc
    31561 ttggcggcat tatatatact gatattgcta aagatggcaa aatgtccgga cctaactttg
    31621 aattaactgg tcaattagta aaggcaacga cgattcctgt cattgcttcc ggtggtatta
    31681 gacatcagca agatattcaa cgattagcgt cgctaaatgt tcacgctgct attataggaa
    31741 aggctgcaca tcaagcatct ttttgggagg ggctaaaatg attaaaaaac gtatcattcc
    31801 atgtttagat gtcaaagatg gtcgtgtcgt taaagggatt caatttaaag gattaaggga
    31861 tattgggaat cctgttgatt tagcaatgta ttacaatgaa gcgggtgctg atgaattagt
    31921 atttttagac atctctaaga cggaagaggg tcatagctta atgctagaag tgattgaaca
    31981 gacagcgtca cgcttgttta tccctcttac tgtagggggt gggattcaaa gtctcgatga
    32041 tattacccaa ttgctaaatc atggtgcaga taaagtatca ttaaattcaa gtgctttaaa
    32101 aaatccacag ctcattaaac aagcgagtga taaattcggt agacaatgca tctgcatagc
    32161 aattgatagc tattatgatc ctgaaagaaa agcacattat tgttgtacga ctggtggtaa
    32221 aaaaatgaca aatattaaag tatatgactg ggtacagcaa gtagaacagt taggtgcagg
    32281 tgagctcctc gttacaagta tgggacatga tggtatgaaa caaggctttg atattgaaca
    32341 cctagcaaat attaagtctc ttgtaaatat tccaatcatt gcttctggtg gtggtggcaa
    32401 tgcacaacac tttgtagaat tatttgatca gacggatgtt tctgcaggtt tagctgcaag
    32461 tatattacat gatcgagaaa cgacggttca atctattaaa gaagtgatac ggcaaggggg
    32521 tatagcagta agatgaccaa atataaaatt gattttagca aaggtttagt gccagcaatt
    32581 ttacaagata atcaaacaaa acaagtattg atgttgggtt atatgaacca agaagctttt
    32641 gataaaacga tagaagatgg tgtggtatgt ttctattcgc ggtcgaaaca acggctatgg
    32701 acaaaaggtg aaacatctgg tcatacgcaa cgtgttaaag atattcatgt agattgcgac
    32761 aatgacacta ttttaattga tgtcatacca aatggaccaa catgtcatac aggcagtcaa
    32821 agttgtttca acacagaagt tccattttca gtgcaaacat tagcgcagac agttcaagat
    32881 agtgcccaat ccaataatga aaagtcatat acaaaatatt tattaacaga aggtatagaa
    32941 aagattacaa aaaaatacgg tgaagaagct tttgaagtcg taattgaagc aattaaaggt
    33001 gacaaaaaag catttgtaag tgaagtagca gatgaacttt atcatttatt tgtcttgatg
    33061 catgcgcttg gcgtcgattt ttcagaaatt gaggcggaat tagcgcgtag acatcataag
    33121 cgcaataact ttaaaggtga acgacaaaat atcgaacagt ggtaaagcaa gtatggacta
    33181 agatataagg aaaaggatca tggcttatac acttacaaat attgtggaaa acgtgacatt
    33241 ttcaagttta aaatacgaca ccaacatatt ttaactatga atgctgtgat ggtactaaag
    33301 ttgcgaactc gttatagata agtagtggat aatcacaata cgaaatcaaa aataattata
    33361 aaaagtaaat tgagcaactc aggaatagat gtcactgtta aagatgtcga aaagtatatg
    33421 aatcgatata atgaagttat gaagggaaaa aatggcgaaa aagctaaaga gttatgtttg
    33481 tcgttactac ctattaatat catagttgtc tttacattct ttgtatttat actataaata
    33541 caaatatatc tagcctgaaa tagaaatgtc atagcctatt taaaagacaa tctccattag
    33601 aactaagata tgcatcccga aagttagact aaaaaactaa ctttatggga tgtattttta
    33661 tgctaatcat cataaattcg agattaagtt aaaggtagtt caagagtaat taaacaataa
    33721 attaaaaata gtaggatact tactttgagg gaagaaaatt aactgtatat atttagttta
    33781 ggaacaagta ttacggttta tcctgataca attattgtgg atgggatgat atttttaggt
    33841 ttaaaatacg acaccagcaa acataataac tgtaatagct cataaatctc cccatatagc
    33901 taatctaaaa aaataataca tcattggaat taagccccaa gcatgtaaat attaaaaatc
    33961 aaaaaagata tatgtaaaaa agttacaatt tgcataatta aattgtgtct aattattgac
    34021 taatt
//
LOCUS       DALAAR010000025        33574 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000025 DALAAR010000000
VERSION     DALAAR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.025"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            102..194
                     /locus_tag="O2458_001220"
     CDS             102..194
                     /locus_tag="O2458_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887087.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            326..559
                     /locus_tag="O2458_001221"
     CDS             326..559
                     /locus_tag="O2458_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCZ3887088.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            670..1935
                     /gene="murA"
                     /locus_tag="O2458_001222"
     CDS             670..1935
                     /gene="murA"
                     /locus_tag="O2458_001222"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ3887089.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1969..2409
                     /gene="fabZ"
                     /locus_tag="O2458_001223"
     CDS             1969..2409
                     /gene="fabZ"
                     /locus_tag="O2458_001223"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCZ3887090.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2468..2908)
                     /locus_tag="O2458_001224"
     CDS             complement(2468..2908)
                     /locus_tag="O2458_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCZ3887091.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            3102..3449
                     /locus_tag="O2458_001225"
     CDS             3102..3449
                     /locus_tag="O2458_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ3887092.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            3886..4581
                     /gene="sceD"
                     /locus_tag="O2458_001226"
     CDS             3886..4581
                     /gene="sceD"
                     /locus_tag="O2458_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCZ3887093.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4785..4887
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4967..5656
                     /gene="tenA"
                     /locus_tag="O2458_001227"
     CDS             4967..5656
                     /gene="tenA"
                     /locus_tag="O2458_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCZ3887094.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5649..6479
                     /gene="thiD"
                     /locus_tag="O2458_001228"
     CDS             5649..6479
                     /gene="thiD"
                     /locus_tag="O2458_001228"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ3887095.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            6463..7254
                     /gene="thiM"
                     /locus_tag="O2458_001229"
     CDS             6463..7254
                     /gene="thiM"
                     /locus_tag="O2458_001229"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCZ3887096.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            7256..7897
                     /locus_tag="O2458_001230"
     CDS             7256..7897
                     /locus_tag="O2458_001230"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCZ3887097.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7983..8855
                     /gene="yidC"
                     /locus_tag="O2458_001231"
     CDS             7983..8855
                     /gene="yidC"
                     /locus_tag="O2458_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCZ3887098.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(9668..10315)
                     /locus_tag="O2458_001232"
     CDS             complement(9668..10315)
                     /locus_tag="O2458_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ3887099.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(10343..11827)
                     /gene="cls"
                     /locus_tag="O2458_001233"
     CDS             complement(10343..11827)
                     /gene="cls"
                     /locus_tag="O2458_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ3887100.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            11993..12286
                     /gene="csoR"
                     /locus_tag="O2458_001234"
     CDS             11993..12286
                     /gene="csoR"
                     /locus_tag="O2458_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCZ3887101.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            12298..12507
                     /locus_tag="O2458_001235"
     CDS             12298..12507
                     /locus_tag="O2458_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCZ3887102.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            12635..12772
                     /locus_tag="O2458_001236"
     CDS             12635..12772
                     /locus_tag="O2458_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887103.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(13036..14238)
                     /locus_tag="O2458_001237"
     CDS             complement(13036..14238)
                     /locus_tag="O2458_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCZ3887104.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            14556..15626
                     /locus_tag="O2458_001238"
     CDS             14556..15626
                     /locus_tag="O2458_001238"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCZ3887105.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            15641..16999
                     /locus_tag="O2458_001239"
     CDS             15641..16999
                     /locus_tag="O2458_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCZ3887106.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            17516..19036
                     /locus_tag="O2458_001240"
     CDS             17516..19036
                     /locus_tag="O2458_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ3887107.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(19405..20100)
                     /locus_tag="O2458_001241"
     CDS             complement(19405..20100)
                     /locus_tag="O2458_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3887108.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(20100..22757)
                     /locus_tag="O2458_001242"
     CDS             complement(20100..22757)
                     /locus_tag="O2458_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCZ3887109.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            22895..23005
                     /gene="kdpF"
                     /locus_tag="O2458_001243"
     CDS             22895..23005
                     /gene="kdpF"
                     /locus_tag="O2458_001243"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCZ3887110.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            23029..24705
                     /gene="kdpA"
                     /locus_tag="O2458_001244"
     CDS             23029..24705
                     /gene="kdpA"
                     /locus_tag="O2458_001244"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCZ3887111.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            24724..26751
                     /gene="kdpB"
                     /locus_tag="O2458_001245"
     CDS             24724..26751
                     /gene="kdpB"
                     /locus_tag="O2458_001245"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCZ3887112.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            26771..27331
                     /locus_tag="O2458_001246"
     CDS             26771..27331
                     /locus_tag="O2458_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCZ3887113.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            27539..28018
                     /locus_tag="O2458_001247"
     CDS             27539..28018
                     /locus_tag="O2458_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887114.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            28011..29594
                     /locus_tag="O2458_001248"
     CDS             28011..29594
                     /locus_tag="O2458_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ3887115.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            29581..30072
                     /locus_tag="O2458_001249"
     CDS             29581..30072
                     /locus_tag="O2458_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ3887116.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            30076..30435
                     /locus_tag="O2458_001250"
     CDS             30076..30435
                     /locus_tag="O2458_001250"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCZ3887117.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            30501..31649
                     /gene="alr"
                     /locus_tag="O2458_001251"
     CDS             30501..31649
                     /gene="alr"
                     /locus_tag="O2458_001251"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ3887118.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            31734..31904
                     /gene="mazE"
                     /locus_tag="O2458_001252"
     CDS             31734..31904
                     /gene="mazE"
                     /locus_tag="O2458_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCZ3887119.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            31901..32263
                     /locus_tag="O2458_001253"
     CDS             31901..32263
                     /locus_tag="O2458_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ3887120.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            32612..>33574
                     /locus_tag="O2458_001254"
     CDS             32612..>33574
                     /locus_tag="O2458_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCZ3887121.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKK"
BASE COUNT        11738 a         4953 c         6104 g        10779 t
ORIGIN      
        1 gggccccgtc aactactgcc aaatacaaca ctatagagtc tagacattga tttatgtccg
       61 actcccaaga atagttttac ttttttacaa tcactaatag attgctaaaa tcaaaatttc
      121 cttcaccact atctacagtc gacatttcat tttttgaaat tatctacatt ttttcatacc
      181 aagatatttt atagttatga tatttatgta aaaagaatta tatagtaagt tagcttaaac
      241 tttactaaaa acgggtatta aactttgtat cattatttaa atttttcatg tacaatgtaa
      301 tacagtaatc ttatgaggtg ataaaatgga ttatatcgga caatatgcag ttatccattt
      361 agtgttacat gttgtatgta tttgtattgc ctattgggct ttacaatcaa ttagattaga
      421 tcaatttttt aaaaaaggat acgccactca attacaagtg tgtatgatat ttgttgctat
      481 tttattaggc actgcagtaa gcaattttat tgtagatttg ttacaatact cgacgcaggt
      541 aaaatattta ataaaataag tctaactcta tgatttgtaa tcaaaactag atataattaa
      601 ataatgactt aaaataattt taaaataggg aaatgtaaag taataggagt tctaagtgga
      661 ggatttacga tggataaaat agtaatcaaa ggtggaaata aattaacggg tgaagttaaa
      721 gtagaaggtg ctaaaaatgc agtattacca atattgacag catctttatt agcttctgat
      781 aaaccgagca aattagttaa tgttccagct ttaagtgatg tagaaacaat aaataatgta
      841 ttaacaactt taaatgctga cgttacatac aaaaaggacg aaaatgctgt tgtcgttgat
      901 gcaacaaaga ctctaaatga agaggcacca tatgaatatg ttagtaaaat gcgtgcaagt
      961 attttagtta tgggacctct tttagcaaga ctaggacatg ctattgttgc attgcctggt
     1021 ggttgtgcaa ttggaagtag accgattgag caacacatta aaggttttga agctttaggc
     1081 gcagaaattc atcttgaaaa tggtaatatt tatgctaatg ctaaagatgg attaaaaggt
     1141 acatcaattc atttagattt tccaagtgta ggagcaacac aaaatattat tatggcagca
     1201 tcattagcta agggtaagac tttaattgaa aatgcagcta aagaacctga aattgtcgat
     1261 ttagcaaact acattaatga aatgggtggt agaattactg gtgctggtac agacacaatt
     1321 acaatcaatg gtgtagaatc attacatggt gtagaacatg ctatcattcc agatagaatt
     1381 gaagcaggca cattactaat cgctggtgct ataacgcgtg gtgatatttt tgtacgtgat
     1441 gcaatcaaag aacatatggc gagtttagtc tataaactag aagaaatggg cgttgaattg
     1501 gactatcaag aagatggtat tcgtgtacgt gctgaagggg aattacaacc tgtagacatc
     1561 aaaactctac cacatcctgg attcccgact gatatgcaat cacaaatgat ggcattgtta
     1621 ttaacggcaa atggtcataa agtcgtaacc gaaactgttt ttgaaaaccg ttttatgcat
     1681 gttgcagagt tcaaacgtat gaatgctaat atcaatgtag aaggtcgtag tgctaaactt
     1741 gaaggtaaaa gtcaattgca aggtgcacaa gttaaagcga ctgatttaag agcagcagcc
     1801 gccttaattt tagctggatt agttgctgat ggtaaaacaa gcgttactga attaacgcac
     1861 ctagatagag gctatgttga cttacacggt aaattgaagc aattaggtgc agacattgaa
     1921 cgtattaacg attaattcag taaattaata taatggagga tttcaaccat ggaaacaatt
     1981 tttgattata accaaattaa acaaattata cctcacagac agccattttt attaattgat
     2041 aaagtagttg aatatgaaga aggtcaacgt tgtgtggcta ttaaacaagt atcaggaaac
     2101 gaaccattct ttcaagggca ttttcctgag tatgcggtaa tgccaggcgt attaattact
     2161 gaagcgttag ctcaaacagg tgcggtagct attttaaata gtgaagaaaa taaaggtaaa
     2221 atcgctttat ttgctggtat tgataaatgt cgttttaaac gtcaagtagt acctggtgat
     2281 actttaacgt tggaagtaga aatcactaaa attaaaggac caataggtaa aggtaatgct
     2341 aaagctactg tcgatggtca acttgcttgt agttgtgaac ttacatttgc aattcaagat
     2401 gtaaaataaa acaaaaaaaa cattcaaaga tttaatgtgt tggcataatc tttgaatgtt
     2461 ttttatttta ctcttctaat ttttcatcct ttaactttgg tttagactgc atcattcgat
     2521 taaatgattt ttttaattct tcaccagata atccatcatc aataagttgt tctaataaac
     2581 tttcagcata ctgttgacgt aaagtgtaaa tatgacattc aaatacgata gacattgttg
     2641 ttttaaaatt tttaatactt ttcacagtag attcaaataa agctggatca ttggctggac
     2701 gcgtgatgac gacacgaata tccaataact gttcattgtc ttgatattcc ttaaatgttt
     2761 cataatgctc attagaaatg acaatttcaa gcaaccaacc cgtgccactt tcctctttat
     2821 taattattac gccatcttct aattcatatt ctataattct gccgtgttca ttcacaattt
     2881 gaaaccttac tgctttaaaa gttttcatat tcccacatcc cattagttta atatttatga
     2941 tttttgaatt atttagaaga tttaaggtca tttcatgact aatttcttta aatataacaa
     3001 ataacgagat gctatagcta ttaattatat aaaatttgcc attttgacca tttttaagct
     3061 agtaattaag atataggcat aggaggtgaa tcacacacta catgctaaat aaaatcgtaa
     3121 ttgtcgggag actgacgaaa gacgcacaaa tatttgaaaa ggaggataga aaaattgcaa
     3181 cgttttgtgt tgcaacgcac cgaaattata aagatgaaaa tggagaaatc gtctgtgatt
     3241 acttattctg taaagcattt ggcaagttag cttctaatat agaaaaatat actaatcaag
     3301 gtacattggt tggtataact ggtcaaatga gatcaagaaa gtatgataaa gacggacaaa
     3361 cacactttgt cactgaatta tatgttgaaa caataaaatt tatgtcccct aaatcccaaa
     3421 ataatgaaat tctctcagat agtatttaga tattgactct caaaatatag ataatcatga
     3481 cttattagaa atttaaatcc aattatccta aacatcctta atatacattt aatccatcct
     3541 aaatttttta tagatctaat taacttgaaa tatactcact taaaaacgac ttacatgact
     3601 agtactgttg tgtaggtcgt ctttttgtgt agtttttcta atagaaaata gacatagatt
     3661 gcaaaacgat tacataaaaa attgcaaata aaaaaacatt tttactgtca tgacaattct
     3721 cgatagaatc atatctgaaa ttatggggga tttcgttgta atcactgtgt aaaaaacaag
     3781 gctatttttt tattttgttg taacctgcac ggattttata gatgttacag tagtctctgt
     3841 aaaactttta gagaaacaaa ttacttgtag gaggatttta agattatgaa gaaaacatta
     3901 ctcgcatcat cattagcagt aggtttagga atcgtagcag gaaatgcagg tcacgaagcc
     3961 catgcaagtg aagcggactt aaataaagca tctttagcgc aaatggcgca atcaaatgat
     4021 caaacattaa atcaaaaacc aattgaagct ggggcttata attatacatt tgactatgaa
     4081 gggtttactt atcactttga atcagatggt acacactttg cttggaatta ccatgcaaca
     4141 ggtactaatg gagcagacat gagtgcacaa gcacctgcaa ctaataatgt tgcaccatca
     4201 gctgttcaag ctaatcaagt acaatcacaa gaagttgaag caccacaaaa tgctcaaact
     4261 caacaaccac aagcatcaac atcaaacaat tcacaagtta ctgcaacacc aactgaatca
     4321 aaatcatcag aaggttcatc agtaaatgtg aatgctcatc taaaacaaat tgctcaacgt
     4381 gaatcaggtg gcaatattca tgctgtaaat ccaacatcag gtgcagctgg taagtatcaa
     4441 ttcttacaat caacttggga ttcagtagca cctgctaaat ataaaggtgt atcaccagca
     4501 aatgctcctg aaagtgttca agatgccgca gcagtaaaat tatataacac tggtggtgct
     4561 ggacattggg ttactgcata agccatttat gcatagctaa ttaatagtta tataagtaac
     4621 ttttagattg gaatatatcg gggaattctc gatatgttcc ggttttttta tttcgcaaat
     4681 atatgtcatt atagaattat tatttaaaga taataataca actgagcaac aataattgaa
     4741 aatgttgaaa ccgtttgcta agattaattt gcaattgaat actaaggaac tactagggga
     4801 gcctaatgat atggctgaga tgaattgttc agacccttat gacctgattt ggttagtacc
     4861 aacgtaggaa agtagttatt gaagtgatgg ggctagtata gcaacattgc catcaaacta
     4921 ctttcatgtt ggaagtagtt tttttattta gggaggattt tttacaatgg aattttcaca
     4981 aaaattgtac caagctgcaa agccaatcat taatgatatt tatgaggatg actttattca
     5041 gaagatgtta tccggagata taggagctga cgcattacgc cattacttaa aagctgatgc
     5101 tgcgtattta aaagaattta caaacttata tgctttgtta attccaaaaa tgaatagcat
     5161 gaatgacgta aaatttttag tagagcaaat agaatttatg gttgaaggtg aagtacttgc
     5221 gcatgatatt ttagcgcaaa ttgttggtga atcatacgaa gaaatcatta aaacaaaagt
     5281 ttggcctcca agtggcgacc attacataaa acatatgtat ttccaagcgc atagtcgtga
     5341 aaatgctatt tatacaattg ctgcaatggc accttgtcca tatatttatg cagaattagc
     5401 taagcgctct caaagtgatc ataagcttaa tagagaaaaa gatacggcaa aatggtttga
     5461 tttttatagt actgaaatgg atgacataat taacgtattt gaggcattaa tgaataaatt
     5521 agccgaatca atgtctgaca aggaattgga acaagtaaag caagtatttt tagaaagttg
     5581 catacatgag cgtcgatttt tcaatatggc tatgacacta gaacaatggg aatttggagg
     5641 aaaagtaaat gattaaacct aaaatagcat taaccattgc aggtactgat ccaacaggtg
     5701 gtgccggcgt aatggctgat ttaaaatcat ttcattcatg tggtgtatat ggtatgggcg
     5761 tcgttacaag tattgttgct caaaatacat tgggcgtaca acatattcat aatttaaatc
     5821 atcaatgggt agatgaacaa cttgatagtg tcttcaatga taccttacct catgctatta
     5881 aaacggggat gattgctaca gcagatacta tggaaacgat tcgtcattat ttaatgcaac
     5941 atgaatctat tccatatgta attgatcctg ttatgttggc gaaaagcggt gattcattaa
     6001 tggataatga cacaaagcaa aacttgcagc atacgttatt gccattagct gacgtagtaa
     6061 caccgaattt accagaagct gaagaaataa cgggactaac cattgatagt gaagaaaaaa
     6121 ttatgcaggc tggccgcatc tttattaatg agattggtag taaaggtgtc atcattaaag
     6181 gcggtcattc aaatgatact gatatagcaa aagattattt atttactaac gaaggtgttc
     6241 aaacatttga aaatgaacga tttaaaacaa aacatacgca tggaacaggg tgtacatttt
     6301 cagcagttat aacggcagaa cttgcaaaag gtagaccatt atttgaggct gtacacaagg
     6361 ctaaaaagtt tatttcaatg agtatacaat atacgcctga aatcggccgt ggtagaggtc
     6421 cagtgaatca ttttgcatat ttaaagaaag agggattaga cgatgaatta tctaaataaa
     6481 atacgtattg aaaacccatt aacaatttgt tatacaaacg atgtagttaa aaattttaca
     6541 gcgaatggtt tattaagtat tggtgctagc cctgcaatga gtgaagctcc cgaagaagct
     6601 gaagaatttt acaaagttgc acaagcgcta ttaatcaata tcggtacttt aacagcagaa
     6661 aatgaacaag atattattgc gattgctcaa acggcaaatg aggcaggctt acctattgta
     6721 tttgaccctg tagctgttgg tgcttctaca tatcgaaagc aattttgtaa attattattg
     6781 aaatcagcga aagtatcagt aattaaaggc aatgcatctg aaatattagc gttgattgat
     6841 gatacagcaa ctatgaaagg tacagatagt gatgctaatc ttgatgcggt tgcaatagcg
     6901 aaaaaggctt acgcaacata taaaactgca atagtaatca caggtaaaga ggacgttatt
     6961 gttcaagata ataaagcctt cgtattagct aatggatctc cattattagc acgagtaact
     7021 ggagctggtt gtttattagg aggcgttatt gctggatttt tatttagaga aacagaacca
     7081 gacatagaag cgttaattga agcggtaagc gtatttaata ttgctgctga ggtagctgct
     7141 gaaaatgaaa attgtggtgg tcctggtacg ttttcaccat tgttgcttga tacgttatat
     7201 catttaaatg aaacaaccta tcaacaacgg attcgtattc aagaggtgga ataatatgtt
     7261 taaccaatcg tatctaaatg tgtattttat ttgtgggacc tccgatgttc cgagtcatcg
     7321 aactattcat gaagtattag aagcagcatt aaaggcaggg attacacttt tccaatttcg
     7381 agaaaagggt gaatcagctt taaaaggaaa cgataaactt gtattagcta aagaattaca
     7441 gcatctctgt catcaatatg atgtgccttt tatcgttaat gatgatgtat ccttggcaaa
     7501 agaaattaac gcggatggta tacatgtcgg tcaagatgat gcgaaagtca aagagatagc
     7561 acaatatttc actgataaaa ttattggact tagtattagt gatttagatg agtatgcaaa
     7621 atctgattta actcatgttg attatatagg tgttggacca atctatccta cgccgtcgaa
     7681 gcatgacgcg catataccag taggtccaga aatgattgca acgtttaaag aaatgaatcc
     7741 gcaactaccg attgtagcta ttggcggtat caatactaat aatgtggcgc cgattgtaga
     7801 agcaggtgcc aatggaattt cagtcatttc agccatttct aaaagtgaaa atattgaaaa
     7861 gactgttaat cgattcaaag atttttttaa taattagtta aatttttcca cttattgttt
     7921 catacgtact cattaatgtg atagaataaa tcctatgtga atataatgat agaggtggaa
     7981 aaatgaagaa aaaagcgtta ctaccattat ttttaggtat tatggtcttt ttggctggtt
     8041 gtgactattc taaacctgaa aaacgtagtg ggtttttcta caatacattc gtagatccaa
     8101 tgaaaaatgt attggattgg ttgggaaata acttattaaa cgacaattat ggtttagcta
     8161 ttattatcct tgtattggta attcgtatta ttttattacc attcatgttg tcaaactata
     8221 aaaatagtca tatgatgcgt caaaaaatga aagttgcaaa gccagaagtt gaaaaaattc
     8281 aagaaaaagt gaaacgtgcg cgtacacaag aagaaaaaat ggctgcaaac caagaattaa
     8341 tgcaagtata taaaaagtat gacatgaacc cgattaagag tatgttgggt tgtttaccaa
     8401 tgctaatcca attaccaatc atcatgggat tatactttgt acttaaagac caacttgtag
     8461 atggtttgtt taaatatcca cacttcttat ggttcgattt aggacgtcct gatatttgga
     8521 ttacaattat tgccggtgtt ttatacttta tccaagcata tgtatcaagt aaaacgatgc
     8581 cagacgaaca acgtcaaatg ggttacatga tgatggtcat ttcaccaatt atgattatct
     8641 ggatttcatt aagctcagca tcagcacttg gtttgtactg gtcagtcagt gcggcgttcc
     8701 ttgtagttca aacacacttt gcgaacattt attatgaaaa agtcgctaaa aaagaagtac
     8761 aacctttcat tgaagcgtat gaaagagagc acaacggcgg cagcaataaa aaaggcaaaa
     8821 acacacaagt tgtgtctaaa aagaaaaaga aataacctta gcaagtccaa ataatgtgca
     8881 ccctaaaagt tgaagataat tttaacttta tagggcgcac attatttttc gcttcaatag
     8941 cgaaacttag tgtcacatta tacaatatgt gataaataga aacagcagta agatgatttc
     9001 aaatgtgaaa gtcatcctac tgctgttttt atttgaaata atattaacga gttttcagta
     9061 tgtaaaaagt ggttaataac agagttgtca ttcttcatcg caatgtcatc ttgacacaat
     9121 gattcatcag tgtagcagta tatgtcgcaa atttccacag caattcactt atacatgtaa
     9181 cagtgtgaaa taataacgtc acaacggcat aatacacatt gcaacgtccc aacaacatat
     9241 catagaatcg taacggcatg acatcaacac caaacctggc tataacacca taacagcgca
     9301 acgccacatc atagcactac atcaagtcgc atcgtagcac cagcacatca aagtaacatg
     9361 gcatcaacat caaaccggtc tataacacca taacaccgca atgccacatc atagcactac
     9421 atcaagtcgc atcgtggtac cagcacatca aagtaacatg gcatcaacgc caaacctgtc
     9481 tataacacca taacaccgca atgccacatc atagcactac atcaagacgc atcgtggtac
     9541 cagcacatca aagtagcatg acatgtcacc aagctcccac aacaacacat caaagtaaca
     9601 tggcatgaca ccaagcttcc acaacaacac gccattacat cgcatcatca tactacaact
     9661 tcaatgtcta gtcgtgacaa ttccattccg taaaaaactg tttcaagtac atcatcataa
     9721 agtcgtgacg ttctttagca atcatcttcg ccgtatctgt atgcattaaa gattctaact
     9781 taagtaattt ttcaaagaaa tgtttaattg cagatggtgg caactgttca actaaatcat
     9841 cattaatctt atctagtgac atatgttctg tccacatagg ttcaccaaag tgtcctgcaa
     9901 attgaaatgt tcgtgcaaca cctatagcgc ctatagcatc aagacgatct gcatccctga
     9961 caatttgacc ttctaaagat aaagtgacat gatcattttt gccattgcga tagctcatat
    10021 tattaataat aaataaaatg tgctcttgat cttcggttga tagtgataaa gaagataaaa
    10081 atgacttcaa ttcaacatat tgtttgttag catctacaac tttttcgtca acggtatcat
    10141 gaagcaaaca tgcgagttca atgactaaag tatcattaac accttcattt tcagcgattg
    10201 atttagctaa agcagtgaca cgatatacat gcgcaatatc atggcctgta taatcatttt
    10261 gatgaataga ttccatatat tttttggcag cactaatgac gtctgattgt tgcattaaag
    10321 ttacactcct catatttcta ttttataaga taggtgacaa taattgtgaa ataccttctt
    10381 taaatttaat ccaaagactt cgcttagcat aacgtgcttt tgttaattca gaagatactg
    10441 ctaaatcatc tataaaagct tgttttaatt ttttggcaat ttgttggtca taaataaaag
    10501 cgttgacttc gaaattcaat gtgaaactgc gatggtccat attagctgtt cccacacttg
    10561 caatttcatc atctataaca agtgtttttg agtgtaaaaa gccattgtcg tagtgaaata
    10621 ctttaacacc ggcatctaat aaggatgctg cattttttaa agtagcccaa aaaacaaacg
    10681 gatggtcagg tttattagga atcatgatat tgacatcaac accacctaat gccgcaattt
    10741 taatagaatc taaaaaggct tgatcaggta tgaaataggg agattgaata taaatcgatt
    10801 ttttagcaga tgaaatcatt ttcaaatagc cgtatttaat ctgttcccat tcttcgtcag
    10861 gaccactaga agctatttga acgccaattg ttccaccaga atttacatct gggaaataac
    10921 gatcatcata ggagatgtgg tcacgtgtgg cttgtgaatt ccaatctaga ataaatcgta
    10981 attgcaatgc attcactgca tccccgacaa ttcgtaaatg cgtatctcgc caatagccga
    11041 atttttttga tttacctaag tactcatcac caacattaaa accaccaaca tatccaattt
    11101 gcccatctat tacaacaatt tttcgatggt ttcgattgtt catacgcaag ttaattaaag
    11161 gtaattttga tgggaaaaat gcttcagcat gtccaccttt attgcgaaac gggcgtaagc
    11221 cttttttacg cagtccacga gaacccatgt catcataaag aattttaact tctacacctt
    11281 gttccgcttt tttaccaagt tcatttaaaa tggtacgacc taattcatca ttttgaataa
    11341 tatagtactg aaaatgaata taatcagtag cattacggat gtcttgtatt aggtcatcaa
    11401 atttttcttg gccgtctgtg tatattttta aatcgttgtc tgttgttaaa aatgctgcgt
    11461 tattatataa caacatttga atcatttctt taaattttac aatttgataa ttggaatttg
    11521 aaaagttttc attttttaaa gcagctaatt gctcatcaac gattaactct aatccttttt
    11581 tatcttcctt atcaattttg aaaatttggt cacgttgaat ttgtcgtcct aatagtaagt
    11641 ataaaatgaa gccgaataaa ggcaagaaaa ctaagactag taaccaagcc cagatagaat
    11701 tggcagaacg tctttccatg aaaataatgg taaaggcgaa taataagttt aaaataaatg
    11761 caccaataaa tattgaattt aaaataatat tagaatgctt gagtgcaatg gataataact
    11821 ctatcatttg aaacctccca tcgaaaatct taaacgagta gtatatctta ttaattgtat
    11881 catgaagcaa tttaaatatc atgacaattt agtttgaaaa acacactttg tgttgacgtt
    11941 gtgttttatg ggatatagta tagtaaggaa tgtaaatgaa ggagtgaatg ctatgactga
    12001 acaagataat gcacatcatt ctgaacaaat aaaaacgaat cttaaatcac gtttaaatcg
    12061 aattgaagga caagtgagag cgattaatcg catgattgaa gaagatgtct attgtgatga
    12121 tgtccttacg caaataagag cgacacgttc ggcgttaaac agtgttgcga taaagttatt
    12181 agaacaacat atgaaaagtt gtattatgaa taaagttaat caaggtgctc aggaagaggc
    12241 aatggaagag ttattagtga cttttcaaaa attgattaaa gactaaagga gtttaagatg
    12301 atacatcaaa atacgattta cacagcggga attgaaacag aagaacaagt aagtcaattg
    12361 acagaacgca tttcaaatat gataggtgtt catcaagtga atattaatat aatagatggt
    12421 caagtaactg tatcgtatga gacaccagca aatttgaata gtattgaaaa agaaatctat
    12481 gatgaaggat acaaaattgt attttagggt ataatgtaga gtgcgctata gattttaatt
    12541 ttgaaaataa attaaaaatt ttgtaaatga tgtagtaaag gtatgtcgaa tagggtaaga
    12601 taatattgaa agcgatccaa acgaggtgat aataatgaaa cgtcctgaaa agattcaaaa
    12661 tgtagtcaaa ctattgtcat cattaggtgt gaatattaaa aaaactaaat ctcgtttaga
    12721 cattattaat actttgccag catctaataa agtaagtcac gaattaaaat aattgactgt
    12781 gattgtgaat cacacaccgt taaagctatc gtcggactat tcgatgatag cttttttgac
    12841 tggatttact aaaatgaaag caagtactat ataacaaaat ccctctttag agtgtacatt
    12901 ttacaatgtg tacttcaaag agggattgtt attatcgagt aatatctata tatgacattt
    12961 taaatcgacg acgtaaatag actatataac gtcatcttga aaagttatga tatttgaaac
    13021 tcaaaatagt ttaaattaat tactttttgg atggtataaa tcgacatatc gttttggttc
    13081 atgataataa attgataaga ctatacctat tccagtcatc atactccata gcgcactacc
    13141 accataacta ataaatggta atggaatacc agtgattggt aacaactgaa ttgtcatacc
    13201 aatattttgt aaaatatgga acacaagtaa agtgacgaaa ccaacgataa agattttgtt
    13261 aaattgatct tcaattttcg cagctaatct tattagatgg aagattaaaa ataaaaatat
    13321 taagatcaat atgacagaac cgataaagcc aagttcctct ccaatcactg aaaagataaa
    13381 gtcagtatga ttttcaggta tataaacttc accgtgattg tatcctttac ctagtaactg
    13441 tccagaaccg atagctttaa gtgattcagt taaatgatag ccatcaccac tactatatgt
    13501 ataggggtca agccatgaat tgattcgtcc catttgatac agttggacac ctaataaatt
    13561 ttcaattaat gcgggtgcat atagaatacc taaaatgact gtcattgcac caacaatacc
    13621 tgtaataaag ataggtgcta agatacgcca tgttatacca cttactaaca tcacacctgc
    13681 aataatagca gctaatacta atgtagttcc taggtcattt tgcagtaata ttaaaatact
    13741 tggtactaac gagacaccaa taattttgaa aaataataac aaatcacttt ggaatgattt
    13801 attgaatgtg aattgattat gtctagaaac gacacgcgct aatgctaaaa ttaaaataat
    13861 tttcatgaat tcagatggct gaatactgat agggccaaac gtgtaccaac ttttggcacc
    13921 attgataata ggtgtaatag gtgactcagg aataacgagc aagcctatta ataatagaca
    13981 gattaagaaa tacaataaat atgtataatg tttaatcttt ttaggtgaaa taaacatgat
    14041 gatacctgca aaaattgcac ctaaaatgta ataaaaaatt tgtctgatac cgaaattagc
    14101 actgtattga ccaccgccca ttgccgagtt aataagcaga acactgaaaa ttgctaaaac
    14161 agctatagtg gctactaata cccagtctac tttgcgaagc caatgcttat ccggctgttg
    14221 acgagatgaa taattcattg caaactcctt ttatactcac taatgtttat atcaatttta
    14281 catgactttt taaaaattag ctagaatatc acagtgatat cagctataga tttcaatttg
    14341 aattaggaat aaaatagaag ggaatattgt tctgattata aatgaatcaa catagataca
    14401 gacacataag tcctcgtttt taaaatgcaa aatagcatta aaatgtgata ctattaagat
    14461 tcaaagatgc gaataaatca attaacaata ggactaaatc aatattaatt tatattaagg
    14521 tagcaaaccc tgatatatca ttggaggaaa acgaaatgac aaaagaaaat atttgtatcg
    14581 tttttggagg gaaaagtgca gaacacgaag tatcgattct gacagcacaa aatgtattaa
    14641 atgcaataga taaagacaaa tatcatgttg atatcattta tattaccaat gatggtgatt
    14701 ggagaaagca aaataatatt acagctgaaa ttaaatctac tgatgagctt catttagaaa
    14761 atggagaggc gcttgagatt tcacagctat tgaaagaaag tagttcagga caaccatacg
    14821 atgcagtatt cccattatta catggtccta atggtgaaga tggcacgatt caagggcttt
    14881 ttgaagtttt ggatgtacca tatgtaggaa atggtgtatt gtcagctgca agttctatgg
    14941 acaaacttgt aatgaaacaa ttatttgaac atcgagggtt accacagtta ccttatatta
    15001 gtttcttacg ttctgaatat gaaaaatatg aacataacat tttaaaatta gtaaatgata
    15061 aattaaatta cccagtcttt gttaaacctg ctaacttagg gtcaagtgta ggtatcagta
    15121 aatgtaataa tgaagcggaa cttaaagaag gtattaaaga agcattccaa tttgaccgta
    15181 agcttgttat agaacaaggc gttaacgcac gtgaaattga agtagcagtt ttaggaaatg
    15241 actatcctga agcgacatgg ccaggtgaag tcgtaaaaga tgtcgcgttt tacgattaca
    15301 aatcaaaata taaagatggt aaggttcaat tacaaattcc agctgactta gacgaagatg
    15361 ttcaattaac gcttagaaat atggcattag aggcattcaa agcgacagat tgttctggtt
    15421 tagtccgtgc tgatttcttt gtaacagaag acaaccaaat atatattaat gaaacaaatg
    15481 caatgcctgg atttacggct ttcagtatgt atccaaagtt atgggaaaat atgggcttat
    15541 cttatccaga attgattaca aaacttatcg agcttgctaa agaacgtcac caggataaac
    15601 agaaaaataa atacaaaatt gactaactga ggttgttatt atgattaatg ttacattaaa
    15661 gcaaattcaa tcatggattc cttgtgaaat tgaagatcaa tttttaaatc aagagataaa
    15721 tggagtcaca attgattcac gagcaatttc taaaaatatg ttatttatac catttaaagg
    15781 tgaaaatgtt gacggtcatc gctttgtctc taaagcatta caagatggtg ctggggctgc
    15841 tttttatcaa agagggacac ctatagatga aaatgtaagc gggcctatta tatgggttga
    15901 agacacatta acggcattac aacaattggc acaagcttac ttgagacatg taaaccctaa
    15961 agtaattgcc gtcacagggt ctaatggtaa aacaacgact aaagatatga ttgaaagtgt
    16021 attgcatacc gaatttaaag ttaagaaaac gcaaggtaat tacaataatg aaattggttt
    16081 acctttaact attttggaat tagataatga tactgaaata tcaatattgg agatggggat
    16141 gtcaggtttc catgaaattg aatttctgtc aaacctcgct caaccagata ttgcagttat
    16201 aactaatatt ggtgagtcac atatgcaaga tttaggttcg cgcgagggga ttgctaaagc
    16261 taaatctgaa attacaatag gtctaaaaga taatggtacg tttatatatg atggcgatga
    16321 accattattg aaaccacatg ttaaagaagt tgaaaatgca aaatgtatta gtattggtgt
    16381 tgctactgat aatgcattag tttgttctgt tgatgataga gatactacag gtatttcatt
    16441 tacgattaat aataaagaac attacgatct gccaatatta ggaaagcata atatgaaaaa
    16501 tgcgacgatt gccattgcgg ttggtcatga attaggtttg atatataaca caatctatca
    16561 aaatttaaaa aatgtcagct taactggtat gcgtatggaa caacatacat tagaaaatga
    16621 tattactgtg ataaatgatg cctataatgc aagtcctaca agtatgagag cagctattga
    16681 tacactgagt actttgacag ggcgtcgcat tctaatttta ggagatgttt tagaattagg
    16741 tgaaaatagc aaagaaatgc atatcggtgt aggtaattat ttagaagaaa agcatataga
    16801 tgtgttgtat acgtttggta atgaagcgaa gtatatttat gattcgggcc agcaacatgt
    16861 cgaaaaagca caacacttca attctaaaga cgatatgata gaagttttaa taaacgattt
    16921 aaaagcgcat gaccgtgtat tagttaaagg atcacgtggt atgaaattag aagaagtggt
    16981 aaatgcttta atttcataga gattagtcga gggacctttt acttataaaa atgatttgaa
    17041 ttaatactaa aagattacaa agaagaggtg gttttgtgtg taaatacaaa attgcctttt
    17101 tctttttatg ttaaatctat aaatttgaaa ctaaatcaag gttaattcta tgtacacact
    17161 ttatatagga agtagtttga atgtttatat aatgttttac aaaaagatgt agtattataa
    17221 tgtctaattt cacatgtgtt tcagtaaaat ttgttgtgga atgttaacga tatacgtatt
    17281 ttataaaaaa ttttttataa tgattattcg aatgatgcgt aacgcttaca tcttatctaa
    17341 tgctagcttt ttgacaaaaa tatgacaatc aattaatgtg attctaataa atattcgcaa
    17401 attgctttat tgcgattaaa tttttttggt ggtactatat agaagttgat gaaatattaa
    17461 tgaacttata tgcaaaagta tattgagaaa taaacaggta aaaaggagaa ttattttgca
    17521 aaattttaaa gaactaggga tttcggataa tacggttcag tcacttgaat caatgggatt
    17581 taaagagccg acacctatcc aaaaagacag tatcccttat gcgttacaag gaattgatat
    17641 ccttgggcaa gctcaaaccg gtacaggtaa aacaggagca ttcggtattc ctttaattga
    17701 gaaagtagta gggaaacaag gggttcaatc gttgatttta gcacctacaa gagaattggc
    17761 aatgcaggta gctgaacaat taagagaatt tagccgtgga caaggtgtcc aagttgttac
    17821 tgtattcggt ggtatgccta tcgaacgcca aattaaagcc ttgaaaaaag gcccacaaat
    17881 cgtagtcgga acacctgggc gtgttatcga ccatttaaat cgtcgcacat taaaaacgga
    17941 cggaattcat actttgattt tagatgaagc tgatgaaatg atgaatatgg gattcatcga
    18001 tgatatgaga tttattatgg ataaaattcc agcagtacaa cgtcaaacaa tgttgttctc
    18061 agctacaatg cctaaagcaa tccaagcttt agtacaacaa tttatgaaat caccaaaaat
    18121 cattaagaca atgaataatg aaatgtctga tccacaaatc gaagaattct atacaattgt
    18181 taaagaatta gagaaatttg atacatttac aaatttccta gatgttcatc aacctgaatt
    18241 agcaatcgta ttcggacgta caaaacgtcg tgttgatgaa ttaacaagtg ctttgatttc
    18301 taaaggatat aaagctgaag gtttacatgg tgatattaca caagcgaaac gtttagaagt
    18361 attaaagaaa tttaaaaatg accaaattaa tattttagtc gctactgatg tagcagcaag
    18421 aggactagat atttctggtg tgagtcatgt ttataacttt gatatacctc aagatactga
    18481 aagctataca caccgtattg gtcgtacggg tcgtgctggt aaagaaggta tcgctgtaac
    18541 gtttgttaat ccaatcgaaa tggattatat cagacaaatt gaagatgcaa acggtagaaa
    18601 aatgagtgca cttcgtccac cacatcgtaa agaagtactt caagcacgtg aagatgacat
    18661 caaagaaaaa gttgaaaact ggatgtctaa agagtcagaa tcacgcttga aacgcatttc
    18721 tacagagttg ttaaatgaat ataacgatgt tgatttagtt gctgcacttt tacaagagtt
    18781 agtagaagca aacgatgaag ttgaagttca attaactttt gaaaaaccat tatctcgcaa
    18841 aggccgtaac ggtaaaccaa gtggttctcg taacagaaat agtaagcgtg gtaatcctaa
    18901 atttgacagt aagagtaaac gttcaaaagg atactcaagt aagaagaaaa gtacaaaaaa
    18961 attcgaccgt aaagagaaga gcagcggtgg aagcagacct atgaaaggtc gcacatttgc
    19021 tgaccatcaa aaataattta tagattaaga gcttaaagat gtaatgtctt gagctctttt
    19081 ttgttttcaa taattgattc tctgtagata tcaaagtact aacgttttaa aggttaaata
    19141 tttaattgga ttgagatctg tatgcggtta tatcattctg tgtaaatatg gttctccacc
    19201 aaatgtggtg agtatataat ttaaagaact atttttaaat taagaataat cgaacataaa
    19261 taaactttat gaaatttcag tatcatgttc ttataaaaaa caatagggct ttttgctgac
    19321 gctagtgcgc gataaataat aagttgaata taaaaaagat cactgccaat cattcgttta
    19381 atggcagcga tcttttttat ttaattattt ctctttccac tgcaacattt gataaccaat
    19441 gcgtggatgt gttttaataa tatcttttgc gtcctcatga cattgtgaaa gtttttgtct
    19501 taatgatgtc atatgcactc ttaaactagg catttcagtc ttattaacat agccatatat
    19561 tcgttttaat atcatctcat aagtgagtac ttttccttta tgatttgaca atagttctaa
    19621 caagctaaat tcatttggcg tcaaatgtac ctcctgatta ttaataacaa cagatttgga
    19681 gccaaagtcg atgcttagca aaccgttagt aaatacaatg ttagtttctt gatgtgactt
    19741 agcgattctc tcgatgactc gtattcgtgc ccgaagctca tcaacattaa aaggtttagt
    19801 catatagtca ttcgcaccgt tatctaaagc ttgaataatt gtttgttctt cttgtcttgc
    19861 acttattaca atgataggaa tgtcagtatg ttgcctgatt tctgaaatca aacataatcc
    19921 atctttatct ggtaaaccta aatctaataa aatgacatct ggtttatcaa tttgaatttt
    19981 aaagtgtgct tgtgtggcat tgtcggctgt agttacattg taataatcta aagttaatgc
    20041 aacatcaagt aaatgtgtga ttgcgtgatc atcttcaatt atcaatattt tagattgcat
    20101 tatacgtctc cttcgttaaa gtctgtatat atattgaaat agaatatact gccgtgtggt
    20161 tggttcggtt tatattgtaa gtttgattga tgtttgtgta ggatagtctg tactaaatat
    20221 aagcctagtc ccatgctttc tttttggtta tctttaaaat atttatttga tcctgtgtaa
    20281 aaaggctcga atatcttttg ttgttcttct aaactaattc caggtccttc gtctataacg
    20341 gcaaattcga tttgttcata gctagcataa cgaatagata aattgatttt ggtgtcagta
    20401 gaagtgtgtt taactgcatt ttcaatcaaa ttgaataaag cttgtaaaat caacttactg
    20461 tcaatgtgta taaactgtaa atttactgag gatgatacag ttatacgctt ttttaaatgg
    20521 cgacgttcta aaatcatatc gatttcttct actaattcac taacgagata aggttgcaat
    20581 tttatctgaa catttgatga ctgtaatttt gttaatgata aaatatttgt cactaataga
    20641 tataaatact gactttcttg aaaactatgt acaagtaatt gttccttttc tatgatagac
    20701 atatctttac tatgtgatac taaaatatct aaatttccca taattgttgt taacggtgta
    20761 cgtatgtcat gcgaaattga tcttaaaaaa tttgaatgtg tcagttgacg ttcagcctgt
    20821 aacatggatt ctctcgtttg tttaagtaac gtcacatttt caacggcgag agaaagttca
    20881 tttaacattg attctaatat tgatgcatca tatggattaa tcacttgaga actttggtaa
    20941 tcaatggcta gaatgccttt aatcggagat gtgccaattg gtatcaacca tttattaatg
    21001 cctggaaatg tatctgttgt tgcaccagct tgtctttcat ttttaattac ccagcttaat
    21061 gcttgttcat gctgttgagt cgtattatcg atatggtttt gcaatggtat tgttttaatt
    21121 actttcgatt gattgataac gtatatagta attgattgtt gcaataattg attaatttgg
    21181 tatccagcat ttattagtaa gttttcaact gtataagttt gtttaatcga atcattaaat
    21241 tgaaataata aatctgtacg ataaagttgc tttttagtaa tggagtattg gaatttaatt
    21301 tgttttaata aagcactcgt taaaatactt gttaaaatgc taacgataaa tgtaatagga
    21361 tagtcaaagc gatatacttc aaatgtatat ctaggttccg taaaaaaata attaaaaaca
    21421 aatacgttaa taattgctgc taaaaagcca atgatgaagg accgcgtcca aatggataat
    21481 aaaatgatgc cgatgagaaa aatcattaaa ataatcgtac tagactcatg cttatcaagt
    21541 tgataaatcc acagtcccat cattacacaa attatttgaa tcaaaatcat ttttaacata
    21601 tctatggcga aacgtttgcc tttaggacga tagggtgatt tgttcatttt caattcaaca
    21661 ggtatttgtt tgattggaac gatttcgatt ttatagctat gttcaaagga cattaaatgg
    21721 tcaattaaag gtgtattgaa aaagtctcgc cacttatttc taatatgttg tccgataatt
    21781 aatttggtta catcttgatt tttacaccat tcgtctaatc ctaatgcaac ggtttggcta
    21841 taaactactt ttacttttgc tcctaatgat tttgcaagca tgagatgttg atgcacttgc
    21901 ttttgactat ctttatattg cctgtttttt tcgaatacat ctatataaat agcagtgaac
    21961 ttcgcatgtt ctttttgagc aatatggaat gcctctttaa ttactgcttc attataaatg
    22021 ctcccactaa ttgccacagc aatatgaggt ttgagtgacg ttttatggtt gtgtcggact
    22081 ttttctttat cactcatcaa gtcggcaact gttcttaacg tcaatgtacg cagttcgctt
    22141 aggtgggcat acgtaaagaa attactaaat gctacatcta gcctatcctt tttatatacc
    22201 ttgccagctt ttaagcgttt aattaattgt tcaggtgaga tatctacgac ttctaataca
    22261 tcggcgctca ttatgaaata gtcgggtaca cgttctttaa catgtacacc ggtcatcagt
    22321 tcaatttgac tacttaaact ttcaatatgt tgaatgttca aagtggtatg aacatcgata
    22381 ccatgattta aaatttcttc aatatccata tatcgtttct catgacgatc tctagaaata
    22441 ttcgtatgtg ctaactcatc gataagtact attgtcggcg attcttcgat tatgcggtcg
    22501 acatctaaat attgaaaatg atgactacca tgtttagtaa aattggttgt gattttaggt
    22561 aattgttcag ctaatgcatt tgtttcatca cgctgatgcg gttcaatata tcctatttta
    22621 atatcaacgt tactttgaaa tagttcaatg gcatttgaaa gcatctcaaa tgttttacca
    22681 acacctggac tgtaaccgat gtaaattgtt aaagatccac gcttttttcc tatgtttagc
    22741 gattcagtgt ttgacataac cttcacctcg atagcaatat cataatattc ttcacaatta
    22801 aatgctataa aatttatttc tgcatacaca tcttaatgat ttcttaataa ctaagtttga
    22861 aaattaatat ttctctcata aacaagttgt tacgatgttg ttgtgttgta aggaggttat
    22921 ggcaatgctc atcgttttat tactggtagt tattgcatta gttttgtatt tattttatgc
    22981 cttaattcgt agtgaaaaat tttagaaata tattaagtga gggttatcat ggaaataatt
    23041 ttgtttctaa caatgatggt tatgattacg tatgttttta gtggatatct atatcgcgta
    23101 gcattggtgc aatcgtcaag agtagattta atatttacta gatttgaaaa tatgtgtttt
    23161 aaaattatcg gcacagattt agaacacatg tcagctaaaa catatgtcaa acattttctg
    23221 gcatttaatg gatttatggg gtttataaca tttgtattgc taatagttca gcaatggctt
    23281 tttttaaatc caaatcataa tttaaaccaa tcgatagatt tagcgtttaa tacagcaata
    23341 tcttttttaa caaatagtaa tttacaacac tataacggtg aatcagatgt gacatattta
    23401 acgcaaatga ttgtaatgac atatttaatg tttacatcta gtgcatcagg ttacgccgtt
    23461 tgtatagcga tgttgagacg tttaactgga ttaactaata tcattggtaa tttttatcaa
    23521 gatattgttc ggtttattgt ccgagtactt ttaccattat catgtttaat tagtatttta
    23581 ttgatgactc aaggtgtacc acaaacgttg catgctaatt taatgattcg gactttaagc
    23641 ggacatattc aacatattgc atttggacct attgcatcac ttgaatcaat aaaacatctt
    23701 ggtacgaatg gtggaggatt tttagcagga aattctgcga caccttttga aaatccaaat
    23761 atttggagca attttataga aatgggcagt atgatgttac ttcctatgtc aatgttgttc
    23821 ttatttggtc gcatgttaag tagacatggt aaacgagtac atcgtcatgc gttgatatta
    23881 tttgtcgcaa tgtttttcat ttttatagca attcttacat taactatgtg gagtgagtat
    23941 cgtggtaatc caatactagc gaatttaggc atttatggac cgaatatgga aggtaaagag
    24001 gtacggtttg gagcaggttt gtcagcacta tttacagtta ttacgacggc atttacaacg
    24061 ggttctgtta ataacatgca tgatagctta acgcctatag gtggattagg accaatggta
    24121 ttaatgatgc taaatgttgt atttggtggc gaaggcgtag gactcatgaa tttattgata
    24181 tttgtcttac tgacggtgtt tatatgcagt ttgatggttg gtaaaacacc agaatattta
    24241 aatatgccaa ttggcgcccg tgaaatgaaa tgtattgtct tagtctttct catacaccca
    24301 attttaattt tagtattttc agcacttgct tttatgattc ctggagcaag tgaaagtata
    24361 acgaatccgt cttttcatgg tatttcacaa gttatgtatg aaatgacatc agctgctgcg
    24421 aacaatggat cagggtttga aggactgaaa gatgatacaa cattctggaa tatctctaca
    24481 ggaatcatta tgttgctttc tcgttatata ccaattattt tgcaattaat gattgcatca
    24541 agtttagtga ataaaaaatc ataccatcaa gataaatata caatagcgat tgataaacct
    24601 tattttggcg tatcactaat cgtatttatc gttttactaa gtggtttgac atttattcca
    24661 gtactattac ttggtcctat tggtgaattt ttaactttaa aataaagtaa agggatggaa
    24721 aaaatgcatc atgtaaataa atatttcaat caaacaatgg tgatagaagc tttgaaaatg
    24781 agtttttata agctgaatct taaacaatta ataaaaaatc cgataatgtt tgtcgttgag
    24841 gttggaatga ttttaacgtt gatattaata tgttttcctg acatatttgg aactagctat
    24901 ctatcgcgag gttatttgat tacaattttt atcatattat taataactat attgtttgct
    24961 aatttttcag aagcctttgc tgaaggtcgt ggaaaagcgc aagctgatag tttaagacaa
    25021 gcacaatcaa atttaactgc tcgattgata gaagaaaatg gtgcgtatcg gatagtgaac
    25081 gccacagagt taaaagccgg acaaaatatt cgggtagaaa atggagaaac aatccctgct
    25141 gatggcgttg taataaatgg attagcaacc gttgatgaat ctgcaattac tggtgagtct
    25201 gcacctgtga taaaagaatc aggtggcgat tttgatggag tcattggcgg tacacttgta
    25261 acatctgatt ggttagaaat acgtgttgag agtgaagcgg gtacatcttt tttagataaa
    25321 atgattgcct tagttgaagg ggcggaaaga aataaaacac caaatgaaat tgcacttttt
    25381 acattattaa caacgttaac aattatattt ttagtcgtta ttgtaacgtt atatccaata
    25441 gcatcttacc tgcatttaat tttacctatt gcgatgctca ttgcgttaac agtatgttta
    25501 ataccaacga caatcggtgg tttgttatcg gcgattggta ttgcaggcat ggatcgtgtt
    25561 actcaattca atgtattagc gaaaagtggg cgtgctgtag aagtatgtgg agatgtcgat
    25621 gtaatgattt tagacaagac aggcacaatc acttatggca atcgaatcgc tagtgaattt
    25681 ttaccagtga atcaacaaat gcttgaaaaa ttgatagtcg ctgcttatat gtcatcaatc
    25741 tacgatgata cgccagaagg aaaaagtatt gtacgattag cgaagcagat gtatataaat
    25801 gagctgccta aagacattga tggcacatat aagcctttta cagctgaaac tagaatgagc
    25861 ggaatcatta caaatgaaat atcggtattc aaaggtgcac ctaatagtat gataaatctt
    25921 gtcaaacaac aacaaggcaa tattcctctt aatattgagt ctctctgtat ggatgtatcg
    25981 agtaaaggtg gcacaccatt aatagtcatt gaaaacaatg tcatgcttgg cgtgatttat
    26041 ttaaaagatg tgattaaaga tggtcttgta gaacgattta ctgaattgag aaaaatgggg
    26101 attgaaacag ttatgtgtac aggtgataat gctttaacag cagctacaat tgcaaaagaa
    26161 gccggggttg atagatttgt tgcagagtgt aaacccgaag ataaaattaa agtcatcaaa
    26221 gatgaacaag cgaaaggcca tattgttgcg atgacaggag atggtacaaa tgatgcacct
    26281 gcgctagcac aagcgaatat aggattagct atgaactcag ggacaattag tgctaaagag
    26341 gcagctaatc taattgattt agattcgaat cctacaaagt taatagaggt tgtaaaaata
    26401 ggcaaacaat tattaatgac gagaggtgca ttaacgacat ttagtttggc taatgacgta
    26461 gcaaaatact ttgcaatttt acctgcacta atgatgtcaa caattccaga aatgacttca
    26521 ctaaatatta tgcatttatc gtcacctaaa tctgcaatta tatcagcttt gatattcaat
    26581 gcattaatca ttgttgcctt aattccaatt gccatgaaag gtgtaaaagt aaagggttac
    26641 agcattgata gaatctttat aaataatatg ttgatttatg gattaggtgg attaatcgtc
    26701 ccgtttttag gcattaaatt aatagatatg attgtacaat tttttgtgta aagaggaagg
    26761 agcctgtacc atgaatacga taagaaatag tatttgttta acgataataa ctatggtatt
    26821 atgtggattt ttattcccgt tagctatcac gttaattggt caaatatttt tttatcaaca
    26881 agcaaatggc agtttaatta cgtatgacaa tcgtatagtt ggatcaaaat tgattggaca
    26941 gcactggaca gagacacgat attttcacgg gagaccgagt gcagttgatt ataatatgaa
    27001 tcctgaaaaa ttatataaaa acggcgtatc ttcaggtgga agtaatgaat cgaatggaaa
    27061 caccgaatta atagcacgca tgaaacatca tgttaaattt gggaattcaa atgtaacaat
    27121 agatgctgca acaagttctg ggtcgggctt agatccgcat attacggtcg aaaatgcatt
    27181 gaagcaagca cctcgtattg ctgatgctcg tcatgtatct acttcaagag tggccgactt
    27241 gattcaacat cgtaagcagc gtggtgtgtt aacaaatgat tatgtgaatg tgcttgaact
    27301 aaatatcgct cttgataaga tgaaagattg attatagatg aaaggcatca tgttgataaa
    27361 attatacatg gtgtcttttt agcaattcaa gcactttctg cttaattaaa tttttagatg
    27421 tacctttagg aagaataaat tagcatgaaa tattccatat gctataatta tcatcatata
    27481 aaaataaaat gaaatagatt ataaatccgt ataatttttt atttggggag aggggtatat
    27541 gtcagattat actaagttga ctaagagtcc taagtcagca ttgttgtatt actacattac
    27601 gaatggctta gaatttatat tgagtgtagc tgtgtatgtc attttttatt ttatatggtt
    27661 aagatttgaa tggccacatt acttacttta tattttgttg gttttaggtg ctctgagtac
    27721 attaaaatta tttattaagc ctttatggca atatcattgt cgcttttata aagttgatca
    27781 actaagtgta caatatcgta catcattttt tatatataaa gaagaaacga gtcgaattga
    27841 acgtttacaa tacttgtcga ttaaatctaa tccaattagt aaagtgctta atttgtataa
    27901 agttggattt atgactgcag gtcatactat ttatttaccg atgatgtctc atgacgatgt
    27961 taaaataatc gaagagagaa ctatgtcgaa tttgagaggg gttgaaagtg atgtctgaac
    28021 ctcaaaaatt acatccgatt tcatatttta atggcataat caatgctatc aaacaaaaca
    28081 tagtcgtctt ttttattttc attatctttc aattgaaaga ttttgattat acgaatcccg
    28141 aatcatattt atggataggt attgtatttg tattcttttt aatatcatac atttctcaaa
    28201 ttgtcggaat tatgaacacg cgatattgga ttgaggataa ttatttcatt ttaacaacag
    28261 gtatttttaa taaaaagcgt aaagaattaa atataaaacg tattcagtcc gttgatatga
    28321 cacaaggggt cgtcaatcaa attattggtg gtgtggattt acaaatcaaa acaccaagtg
    28381 atggtattgt cttaagtgtt atttcgaaaa aacaaggtga atatcttgaa cgatatattg
    28441 atcaattgca aacagagttg aagacagaag tggcgcaaaa gtctagtgct gagaagaatg
    28501 acaactgtaa tcatgacatt gaaggtaatg tggaagacat cgcagaaggt cgcttagatg
    28561 aagtgcagaa cactgatagt atgcttaatc agaaaaataa acgcattcct atatatcaga
    28621 tgaattttaa agaatcacta tttatggcaa tgaccagtgg cgccattggt gtaacgttag
    28681 cagctttagt accgatttac ggggctgtaa gagagttgat cccatggtcg aagctaaatc
    28741 atgaaattag tcaatgggtt caaattgtaa gtatttcagt attaatcatc attttactag
    28801 tattaatagt ggcatatatt attggaacga ttataacaat gataagattt tatggctttt
    28861 cggtgatgct tgaaaatgag caacttaaaa ttgaatatgg tctctttaac aagaagaaag
    28921 taactgtacc aacgaaacga ttgcaagctg ttgttgaacg tcagtcatat atacgaagat
    28981 tatttggcta cacagcaata cattttatca tcacaagtga ttttgaaaat aaaaaaatcg
    29041 aagatgatga caatgattca ggtagtgtgc cgatattgcc gtttattaaa cgagacaaag
    29101 catatcaaat tatccagaat ttagtaccag atttatctta tcaacaagtt aatgaaggta
    29161 tgcctttgtc aggtttccac agacactttt taatacctag tattattgtc atagctatgg
    29221 caggtatagg ctgttatttt tggaatcaat tattattgtt atttacgatt ataacaatag
    29281 tcattattgg tttatttgtg atcaaaggat atctttatgt caaacatgcg ggttttaaaa
    29341 tgacagaaga acaaattact atccagaatt tcgagctatt gaaacgaaat atttattatt
    29401 ttaaagcgca tcacattata ggatttgaca cttggcaaca tacattactt aaacgcaaca
    29461 atttaaataa ttttagcttt attgttgcta aaggtgtggg agcccaatcg attaacttaa
    29521 aatacgcaag aagtgaagat gcaaataaat tgaaatcatg gtatttaaga ggtgaaaatg
    29581 atgagggatt ataattttat gtcaccacac gctaaaaaag tgatgaggtt aagtgctgtg
    29641 ctgttttgga tacccatagc gcttgtactt ttaatcgcat ttaatatttt gaactggcta
    29701 ttttgggaga ttttagataa ccatttatcc attgttagta gtgtgattat aatattactt
    29761 gttgctgttt tcacgcttgt tatagcgcca gtctatcgat ataagcactg cagatatgca
    29821 tttgaaaaac accatttgcg tgtacgaaac ggcattttat ttttggatga aaaggtaata
    29881 ccatattttc gtattcaaaa tattgatatc gatgttggtc ctataatgag acgctatcga
    29941 ttagcaactt tgacccttta tacagcgggt ggtcaagctg aaattgaatt aatagataga
    30001 agtgaagcac gtagattgaa acgatggtta aataacgaga gacatttaga aaacaataaa
    30061 aatgaggaat aatcaatgat acatggaatt ggtgtagatt taatcgaaat cgatcgaata
    30121 caagcgttat atagtaagca accaaaattg gttgagcgga ttttaactaa aaatgaacag
    30181 cacaaattca acaatttcac acatgagcaa cgtaaaattg aatttttagc tggcaggttt
    30241 gctacaaaag aagcgttcag taaagcatta ggcacaggct taggaaaaca tgtagctttt
    30301 aacgatatag actgttacaa cgacgaactt ggcaaaccaa agattgatta cgaagggttt
    30361 atcgtacatg ttagtatctc acacactgag cattatgcga tgagccaagt tgttttagaa
    30421 aagtcagcat tttaaatttg ttatacaatg ataattaaga tctagtttta aaacgtgtgg
    30481 gaattacagg aggaagtaat atgtcggata aatattatag atctgcgtat atgaatgtag
    30541 atttaaacgc tgttgcatca aatttcaaag tattcagtac attgcatcca aataaaacag
    30601 tgatggctgt cgttaaagcc aatgcctatg gactaggtag tgttaaagta gcacgtcatt
    30661 taatggaaaa tggcgccaca ttttttgctg tagcaacgtt agatgaagcg atagaactta
    30721 gaatgcatgg gattactgct aaaattttag tcttaggtgt gttaccagct aaagatattg
    30781 ataaagcgat acaacaccga gttgccttaa cggttccgtc taaacagtgg ttgaaagaag
    30841 caattaaaaa catttctggt gagcaagaga aaaagttatg gttgcacatt aaattagata
    30901 caggaatggg acgtttaggt attaaagata ctaaaacgta tcaagaagtg attgaaatca
    30961 ttcaacaata tgagcaactt gtatttgaag gcgtgtttac acactttgcc tgtgctgacg
    31021 aaccaggaga tatgacaact gaacaatatc aacgttttaa agatatggtc aatgaagcaa
    31081 ttaaacctga atatatacat tgtcagaact cagcaggctc tctattaatg gattgccaat
    31141 tctgtaatgc aataagacca ggaatttccc tttatggata ttatccatca gagtatgtac
    31201 agcaaaaagt taaagtacac cttaaaccaa gtgtgcaatt aattgctaat gtagttcaaa
    31261 caaagacgct acaagcgggt gagtctgtaa gttatggtgc aacttataca gctactgacc
    31321 caactacaat agcattgtta cctattggat atgcagatgg ctatttacgc ataatgcaag
    31381 gtagctttgt aaatgtaaat ggtcatcaat gcgaagttat tggtcgcgta tgtatggatc
    31441 agacaattgt taaagtgcca gatcaagtta aagctggaga ttcggtgatt ttaatagata
    31501 atcatagaga aagtccacag tcggtagagg tggtagctga aaagcaacat actattaatt
    31561 atgaagtgct ttgtaacttg tcgagacgtt tgccgcgaat ctatcatgat ggtgatcaac
    31621 gttttgtaac aaatgaattg ttaaaataat atggtcagtg catataaaat ttgttatcat
    31681 tagagtaatt aaaggtcatt taataacttt tggaatcaat tggaggttct catatgttat
    31741 cttttagtca aaatagaagt catagcttag aacaatcttt aaaagaagga tattcacaaa
    31801 tggctgattt aaatctctcc ctagcgaacg aagcttttcc gatagagtgt gaagcatgcg
    31861 attgcaacga aacatattta tcttctaatt caacgaatga atgattagac gaggagatgt
    31921 ttatttagca gatttatcac cagtacaggg atctgaacaa gggggagtca gacctgtagt
    31981 cataattcaa aatgatactg gtaataaata tagtcctaca gttattgttg cggcaataac
    32041 tggtaggatt aataaagcga aaataccgac acatgtagag attgaaaaga aaaagtataa
    32101 gttggataaa gactcagtta tattattaga acaaattcgt acacttgata aaaaacgatt
    32161 gaaagaaaaa ctgacgtact tatccgatga taaaatgaaa gaagtagata atgcactaat
    32221 gattagttta gggctgaatg cagtagctca ccagaaaaat taggcgtcta ttatatgtat
    32281 ttttcagaga taaataaaat attgatataa aagacaataa ctttataata attataacta
    32341 tttctaaatt ctgtacgaag aattttctta taaacaaaga ttttagcaaa taccagttat
    32401 gatattcata ttttttatta taaaaggatg tcttaagttt tttaggcttt aggtattcca
    32461 tcctaaagtt tttttagctt aaaagtatca tctacagcaa aattgcaaac gacaaaattg
    32521 ataagtgcaa ttaaataaat gttagtaagt gaatcataat tatccttgct taagcatttg
    32581 ctttgtaagg gaagtgagga ggcaactaat cgtggaagaa tttaagcaac attataaggg
    32641 tttaattgat gaaagtttaa cgtgccaaga taaagtagaa ttgataaaaa agtgtgagaa
    32701 atacactgac gaagtgattc gtaaggacgt cttgcctgaa gacattgtcg atattcacaa
    32761 aaactatata ttgacgttaa acttaacgcg tgaagatgtg ttcaagacat tagatgtctt
    32821 acaagaaatc gttaaaggct ttggttatag ttatcgagat tatcaaagat tggtagataa
    32881 acttcaagtt cacgataaag agatagactt agcttctagc ttacaacaaa caatgcttaa
    32941 aacagatatt ccacaatttg atagtattca aattggcgtt atttcagtgg cggcacaaaa
    33001 agtaagtgga gattatttta atttaattga ccataacgat ggcacaatga gctttgctgt
    33061 tgcagatgtc attggaaaag gtataccagc tgctttagca atgagtatga taaagtttgg
    33121 catggattct tatggacact cacaattacc gagtgatggt ttaaaacgtt taaatcgtgt
    33181 tgttgaaaag aatattaatc aaaatatgtt cgtcacaatg ttttatggtt tatatgaaga
    33241 aatgaaccat ttattgtatt gtagttcagc tggtcatgag cctggatata tttatcgcgc
    33301 tgaaaaagaa gaatttgaag aaatttcagt tagaggtaga gtgttaggaa tcagttcaca
    33361 aacacgatat caacaacaag aaattccaat ataccttgat gatttaatta tcattttaac
    33421 ggatggtgtg actgaagcta gaaatagtga aggtaccttt atagataaac aaaaactttt
    33481 agaatatatt aaaaaacata aacatatgca cccacaagat attgttcaaa ttatctatga
    33541 agcaatttta aagcttcaaa acccaaataa aaaa
//
LOCUS       DALAAR010000026        32716 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000026 DALAAR010000000
VERSION     DALAAR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.026"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(278..1207)
                     /locus_tag="O2458_001255"
     CDS             complement(278..1207)
                     /locus_tag="O2458_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCZ3887122.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(1204..2040)
                     /locus_tag="O2458_001256"
     CDS             complement(1204..2040)
                     /locus_tag="O2458_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ3887123.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(2034..2777)
                     /locus_tag="O2458_001257"
     CDS             complement(2034..2777)
                     /locus_tag="O2458_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887124.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            2899..3543
                     /locus_tag="O2458_001258"
     CDS             2899..3543
                     /locus_tag="O2458_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCZ3887125.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(3623..4375)
                     /locus_tag="O2458_001259"
     CDS             complement(3623..4375)
                     /locus_tag="O2458_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCZ3887126.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            4611..5375
                     /locus_tag="O2458_001260"
     CDS             4611..5375
                     /locus_tag="O2458_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCZ3887127.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(5436..6230)
                     /gene="tagH"
                     /locus_tag="O2458_001261"
     CDS             complement(5436..6230)
                     /gene="tagH"
                     /locus_tag="O2458_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCZ3887128.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            6577..7389
                     /gene="tagG"
                     /locus_tag="O2458_001262"
     CDS             6577..7389
                     /gene="tagG"
                     /locus_tag="O2458_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCZ3887129.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            7488..8591
                     /locus_tag="O2458_001263"
     CDS             7488..8591
                     /locus_tag="O2458_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ3887130.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            8588..9649
                     /locus_tag="O2458_001264"
     CDS             8588..9649
                     /locus_tag="O2458_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCZ3887131.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            9712..10110
                     /gene="tagD"
                     /locus_tag="O2458_001265"
     CDS             9712..10110
                     /gene="tagD"
                     /locus_tag="O2458_001265"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCZ3887132.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(10227..11522)
                     /gene="pbp4"
                     /locus_tag="O2458_001266"
     CDS             complement(10227..11522)
                     /gene="pbp4"
                     /locus_tag="O2458_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCZ3887133.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            11943..13670
                     /locus_tag="O2458_001267"
     CDS             11943..13670
                     /locus_tag="O2458_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887134.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            14029..15258
                     /locus_tag="O2458_001268"
     CDS             14029..15258
                     /locus_tag="O2458_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ3887135.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            15513..15605
                     /locus_tag="O2458_001269"
     CDS             15513..15605
                     /locus_tag="O2458_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887136.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            15783..16616
                     /locus_tag="O2458_001270"
     CDS             15783..16616
                     /locus_tag="O2458_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCZ3887137.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAKGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            16898..17695
                     /locus_tag="O2458_001271"
     CDS             16898..17695
                     /locus_tag="O2458_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887138.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            17731..18735
                     /locus_tag="O2458_001272"
     CDS             17731..18735
                     /locus_tag="O2458_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ3887139.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            18732..19748
                     /locus_tag="O2458_001273"
     CDS             18732..19748
                     /locus_tag="O2458_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ3887140.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            19982..20950
                     /gene="dhaK"
                     /locus_tag="O2458_001274"
     CDS             19982..20950
                     /gene="dhaK"
                     /locus_tag="O2458_001274"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCZ3887141.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            20992..21576
                     /gene="dhaL"
                     /locus_tag="O2458_001275"
     CDS             20992..21576
                     /gene="dhaL"
                     /locus_tag="O2458_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCZ3887142.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            21569..21931
                     /locus_tag="O2458_001276"
     CDS             21569..21931
                     /locus_tag="O2458_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCZ3887143.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            22047..22544
                     /locus_tag="O2458_001277"
     CDS             22047..22544
                     /locus_tag="O2458_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887144.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            23000..24067
                     /locus_tag="O2458_001278"
     CDS             23000..24067
                     /locus_tag="O2458_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887145.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            24218..25261
                     /locus_tag="O2458_001279"
     CDS             24218..25261
                     /locus_tag="O2458_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3887146.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            25590..26018
                     /locus_tag="O2458_001280"
     CDS             25590..26018
                     /locus_tag="O2458_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887147.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(26227..26733)
                     /locus_tag="O2458_001281"
     CDS             complement(26227..26733)
                     /locus_tag="O2458_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887148.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            26846..27769
                     /gene="graX"
                     /locus_tag="O2458_001282"
                     /gene_synonym="apsX"
     CDS             26846..27769
                     /gene="graX"
                     /locus_tag="O2458_001282"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCZ3887149.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            27785..28459
                     /gene="graR"
                     /locus_tag="O2458_001283"
                     /gene_synonym="apsR"
     CDS             27785..28459
                     /gene="graR"
                     /locus_tag="O2458_001283"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCZ3887150.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            28452..29492
                     /gene="graS"
                     /locus_tag="O2458_001284"
                     /gene_synonym="apsS"
     CDS             28452..29492
                     /gene="graS"
                     /locus_tag="O2458_001284"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCZ3887151.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            29636..30397
                     /gene="vraF"
                     /locus_tag="O2458_001285"
     CDS             29636..30397
                     /gene="vraF"
                     /locus_tag="O2458_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCZ3887152.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            30387..32276
                     /gene="vraG"
                     /locus_tag="O2458_001286"
     CDS             30387..32276
                     /gene="vraG"
                     /locus_tag="O2458_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCZ3887153.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT        11386 a         4568 c         5806 g        10956 t
ORIGIN      
        1 ctttgattta aagagtgaca aatgtttaca gtttaattaa aactgcataa gaacttctag
       61 cttttctctt tcgttcaaag agaagttcta ataccaccat atcgtgcgat cgggaacggt
      121 atatatatta ataggagggt aatatatatt taacgcacga tatgggacta ttagccttcg
      181 actttgttat gttgatgtgt ggcctaaaat attggagata ccaatatttt aggttgcatc
      241 aacatcaatt catcttactt cattaaaaca cagcgtgtta tttcatgctt ccgtgtacag
      301 tttcaatatt tgatttcatc attttgtagt aagagtcacc tttagtgcct tctttaccga
      361 ttgaatctgt gtacacttca ccaaagatat ctttcttcgt ttcttcagat aaactttcca
      421 ttgctttctt atcaacactt gtttctacta ataagtgttt taatttgtgc tttttaacaa
      481 actcaatagc ttgtctcatt tgttcaggtg taccttgttt ttcagtgtta atttcccaaa
      541 tataacctgg tgtaataccg tattgttttg agaagtactt gaaggcacct tcacttgtaa
      601 tcatggcacg ttgttctttt ggaatgtcat taaatttgtc tttactgtca ttatttaatt
      661 tttccaattg agcaatgtat ttgttacctt gcttttcata atctgcttta tgttttttgt
      721 cgttatcgat aaatgtttgt tgaattgttt ttacgtattt aataccgtta tctaaactta
      781 accatgcgtg tggatcttgt ttatctttgt tgccttcttc accgtttaaa tagataggtt
      841 taacatcttt tgatactgcg ataacttttt tatcttttaa tgatttacca gcctgttcta
      901 aggctttttc aaaccaaccg ttaccagtct ctaaatttaa tccgttgtat aaaataacgt
      961 cagcgtcagt taacttttta atatctttag gtttaacttc atattcatga ggatcttgac
     1021 caacaggtac aatactatga atatcgacgt tgtctccacc aacattttta gccatatcat
     1081 ataaaattga attcgtcgtt actactttta atttgccatt tgacttatca ctgctttgtt
     1141 taccaccagt accacatgca gcaactagaa gtaataaggc taataataaa ggtactaatt
     1201 ttttcatgtt aaacttcctc gtttctttct attcgtaaat tttgtgaaaa ataatgtgat
     1261 gatataaatt acaaacgtac aaagtacgat tgtcgcacca ctaggaatgt tgtaaatata
     1321 gctgtaataa agtccgacaa ttgaacttat gacacttatt aaacttgcta taatcatcat
     1381 tgagtatagt tttttactaa ttaaaaatgc tgtagatgca ggtgtaatta ataatgcaac
     1441 tacaagaata atacctaccg tttgaatact tgctactgtt actaatgaga gtaacaacat
     1501 cacaaagtaa tgtaataacg tcgtatttag accactcatt ctactaaacg ttggatcgaa
     1561 tgtagaaatc attaatggac gatagaaaat aatgattaga ataaggacga ttgaaccaat
     1621 cacaatagtt gttaaaaatg cactatttgt gattgccagt aaattaccaa acagaatatg
     1681 gtacaaatct gtcgtagtgt ttattaagct aataataata atccccgaag ctaagaaagc
     1741 ggtaaaacta attccaatag cggcgtcagg tttcgtttta ctactagatg tgatataacc
     1801 gataaaaata cttgcgatca taccagttat aagtgcgcct acaaacattg gaataccaaa
     1861 taagaatgat agggcaacac caggtaatac tgcgtgactc attgcatctc ccattaatga
     1921 aagaccacgt aatacaatta aactaccaac tgtaccacaa actatcccta caataattga
     1981 agttatcaat gctcgattca agaattgata tgtaaataaa tgttcgacaa actctaacat
     2041 gttatattgc tcctttgact agggtcacta cagtcagtgc tactcataaa tgtttcgttt
     2101 aagcgagtga cactcatagc ctcttcacta tcaccaaagt atcgtaatgt ttgatttaat
     2161 agaataatgc gatcaaagta ttgctttgct tttgatagat catggtggat gataagaata
     2221 agttttcctt gttgttttaa gttctcgatt tttgtcatga ttaatttttc gctactaaaa
     2281 tcaattccga caaacggctc atctagaaaa taaacttcac tttcggacat caatgctctt
     2341 gctactagca cacgttgtaa ttgtccacca cttaattctg aaatttgtcg atgacgtaaa
     2401 gattctaatt ctaaatcgct taataactgt ttgagtttat cccttgctga tttattaggt
     2461 cgtctaaacc atccaatttc tttgtagcaa cctgataaaa tcacttgttc cacacttata
     2521 ggaaaatcta aatcaatatg tgctttttgt ggaatatatg taatatgttg cagttgttgt
     2581 tgtataggtt tgttatataa caatttagta ccggtagcat taaattcacc aattaaagac
     2641 ttgataaggg aagatttacc agcaccattc gggcccatga taccaattat ttcgccgcgt
     2701 actggtatcg ataaggaaat gtttttaagt acatgcttat tacctaaaaa cagatttaaa
     2761 tcttttgttt ctaacaaacg tttatacctc ctaattaaaa gtttaggcta acctaattaa
     2821 ttgtataata aactgagaat atttatcatg tcaagtaaat tcgtgatata atatagacaa
     2881 tgtatgtgag gtgaaagtat gttaactgaa gaaaaagagg actatttaaa ggcaatcctt
     2941 acgaataatg gcgataaaaa ctttgtgaca aataaaatct tatctcaatt tttaaatatt
     3001 aagcctccat ctgtaagtga aatggtagga cgtcttgaaa aagcaggcta tgttgaaaca
     3061 aaaccataca aaggtgttag attaacagag gatggtttaa cgcatacgct tgatatcatt
     3121 aagagacatc gactattaga attattttta atagaaatat tgaaatataa ttgggaagaa
     3181 gtacatcaag aagcagaaat tttagaacat cgaatttcag atttatttgt tgaaaggctg
     3241 gatagcctgt taaatttccc agaaacttgc ccgcacggcg gtgtgattcc tagaaataat
     3301 gaatataaag agaaatatat aacaacgatt ttgaattatg aacctggtga tatcgttaca
     3361 atcaaacgtg tgagagataa gaccgatttg ctaatatatt tgtctagtaa agatatttct
     3421 attggtaatg aagtggaaat tgtatcgaaa gatgaaatga ataaagtaat tatcattaaa
     3481 cgtaatgata atgtaattat tgtcagttac gaaaatgcaa tgaacatgtt tgctgaaaaa
     3541 taaaataaag aagccataaa gatatccatg attgaactga taaagacata tggataattg
     3601 ctttaggctt cttttttatt agttaattta tcaagtgagt atatttgagt aaaatattca
     3661 ctgcataaag attgaagata atccagattg tactataaat gaagataggt acatgactga
     3721 gttctttaag tgcactacca tcccactgtg gactcggacg ctggaaagtc aatttagcaa
     3781 tcgtccaact agattgtaga acttcgccta ataatacacc taaaatatat tgataactca
     3841 ttgtgacaag tagttgaatt tctactatat tttcatcttt taatataaaa tacaacatga
     3901 tagaaattaa agttataaca acaatgggtg agccttttct agatgttaaa attaaaaaat
     3961 aaataaatat caataaatag gtaaatataa agaaactagg tatctgataa tggctcgacg
     4021 ctaaacctat caataacata ataggtggca taaaataacc accaatcgtt gtaagccatt
     4081 ggcctgctag atgtctagat tgtgtaattg cgaatccttg ttgtaatgtc tgttgtcgct
     4141 ctcgtggact tgttacaatg actaaatctt ttgcacggcc accagcgagt ttattaaaca
     4201 gtacatgacc aaattcatgt gttaaaacag ggatatagtt taaaatgaca tctaaatagt
     4261 tcaaaacagg cttatgtcta tattgatgaa tagcaatata acaagctgca acaataacga
     4321 taatgtatat attaagttga attgtcgtat taaaaaagtt tgataaataa ttcattgtta
     4381 acctcatata agatattaat ttaaagtttg cttatcactt attataaatg atattggcat
     4441 caatagcgtt agactttaga cttaccttag ttaaactaat tttaattttt gaaaaggtga
     4501 atatgtgtta aaataaagca aaatcatttc gatataaata ggatgaatat aaatactgtt
     4561 aatattgatt acactaacat aataatgaaa taagatagga gattcctgtt atgactgttg
     4621 aagaaagatc caatacagcc aaagttgaca ttttaggggt cgattttgat aatacaacaa
     4681 tgttgcaaat ggttgaaaat attaaaacct tttttgcaaa tcaatcaacg aataatcttt
     4741 ttatagtaac agccaaccct gaaatagtga attacgcgac gacacatcaa gcgtatttag
     4801 agttaataaa tcaagcgagc tatattgttg ctgatgggac aggagtagtc aaagcttcgc
     4861 atcgtttaaa gcaacctcta gcgcatcgta tacctggtat tgagttgatg gatgaatgtt
     4921 tgaaaattgc tcatgtaaat catcaaaaag tatttttgct aggggcaact aatgaagttg
     4981 tagaagcggc acaatatgca ttgcaacaaa gatatccaaa catatcgttt gcacatcatc
     5041 acggttatat tgatttagaa gatgagacag tagtgaaacg aattaaactg tttaaacctg
     5101 attacatatt tgtaggtatg ggattcccta aacaagaaga atggattatg acacatgaaa
     5161 accaatttga atctacagtg atgatgggcg taggtggttc tcttgaagta tttgctgggg
     5221 ctaaaaagag agcgccttat atctttagaa aattaaacat tgaatggata tatagagcat
     5281 taatagattg gaaacgtatt ggtagattaa agagtattcc aatatttatg tataaaatag
     5341 ccaaagcaaa aagaaaaata aaaaaggcga aataatcatg atgacaaaaa taaaaccgag
     5401 gaaatcctta aatggagatt ctcggttttt tcggtttatt taataacgaa gcgggactca
     5461 tcgagtttgt ttctaaattc tttttgttcg gctttggatt tctttttaaa atcgttaagg
     5521 aaagcttcat atttaggtaa tacatcatca agttcaccgt aatcttttaa ctttccgcct
     5581 tcaatccaag caatcttagt acaaaattgt ctcacttgtc ctaagttatg actaacgaaa
     5641 aagatggttt tgttttgctc tttaaactcg taaattttat ctaaacattt ttgtgcaaaa
     5701 gtttggtcac ctacagataa agcttcgtca atgactaaga tatctggatt aactgtgata
     5761 ttaattgaaa aaccaagttt tgcacgcata ccacttgaat actttttaac tggttgataa
     5821 ataaactcac caagttcact aaattcaata atcttaggtg tcatcgcttt aatttctttt
     5881 cgcttaaagc ccatacataa cattttaaat tcgatatttt caatccctgt aagttgtcca
     5941 ctcaagccag cactaattgc gataacgctg acttcaccat tacgatccac tttgccaaca
     6001 gtaggcgaca aagaaccgcc aatgatattg ctcaacgttg atttgccgga accattgatg
     6061 ccaacaagcc ctatgacgtc accttcatat gcttttaaac taatgtcatc taaagcgaaa
     6121 aatgttttgt ttttatgttt gggaatgagc gcatctttca tacgttcttt atttgtacga
     6181 taaatacgat attcttttgt tacattttta atgtttaccg aaacgttcat ttgtagacct
     6241 tccttattca catttatcta gattataata tactactcaa cagttgttaa attttaaaac
     6301 ctgttgtaaa gtgtatagaa gattttgtta ttatcagagt gggtgttttg acacaaaatg
     6361 ttaatcatca atgataacaa tgatatttaa aaactaaact tatttcaact tacatgattg
     6421 tatactataa tgtatttgta ataaactaat attttaaaga actagacaat aattttgata
     6481 gcatccatgt atagtgatag tatttacaac aattattata atactattta gttaagtaga
     6541 gaaatagtta aacatttgaa agtgtggttt aatggaatgt cagcaatagg aacagttttt
     6601 aaagaacatg taaagaactt ttatttaatt caaagactgg ctcagtttca agttaaaatt
     6661 atcaatcata gtaactattt aggtgtggct tgggaattaa ttaaccctgt tatgcaaatt
     6721 atggtttact ggatggtttt tggattagga ataagaagta atgcaccaat tcatggtgta
     6781 ccttttgttt attggttatt ggttggtatc agtatgtggt tcttcatcaa ccaaggtatt
     6841 ttagaaggta ctaaagcaat tacacaaaag tttaatcaag tatcgaaaat gaacttcccg
     6901 ttatcgataa taccgacata tattgtgaca agtagatttt atggacattt aggcttactt
     6961 ttacttgtga taattgcatg tatgtttact ggtatttatc catcaataca tatcattcaa
     7021 ttattgatat atgtaccgtt ttgttttttc ttaactgcct cggtgacgtt attaacatca
     7081 acactcggtg tgttagttag agatacacaa atgttaatgc aagcaatatt aagaatatta
     7141 ttttactttt caccaatttt gtggctacca aagaaccatg gtatcagtgg tttaattcat
     7201 gaaatgatga aatataatcc agtttacttt attgctgaat cataccgtgc agcaatttta
     7261 tatcacgaat ggtatttcat ggatcattgg aaattaatgt tatacaattt cggtattgtt
     7321 gccattttct ttgcaattgg tgcgtactta cacatgaaat atagagatca atttgcagac
     7381 ttcttgtaat atatttatat gacgaaaccc cgctaaccat taataaatgg aagtggggtt
     7441 catttttgtt tataatttaa gtaaataaca tattaagttg gtgtattatg aacgttttaa
     7501 taaagaaatt ttatcatttg gtagttcgaa tactttctaa aatgattacg cctcaagtga
     7561 ttgataaacc gcatatcgta tttatgatga cttttccaga agatattaag cctatcatca
     7621 aagcattaaa taattcgtcg tatcagaaaa ctgttttaac aacaccaaaa caagcgcctt
     7681 atttatctga acttagcgac gatgttgatg tgatagaaat gactaatcga acattggtaa
     7741 aacaaattaa ggctttgaaa agcgcgcaga tgattattat cgataattat tacctattac
     7801 taggtggata taataagact tctaatcaac acattgttca aacgtggcat gcaagtggtg
     7861 cattaaaaaa ctttggctta acagatcatc aagtcgatgt gtctgacaag gcaatggttc
     7921 agcagtaccg taaagtttat caagcgacgg atttttactt agtgggttgt gaacaaatgt
     7981 cacaatgttt taaacagtct ttaggtgcaa cagaagagca aatgctgtat tttgggcttc
     8041 cgagaattaa taaatattac acagctgata gagcaacggt taaggcagag ttaaaggata
     8101 aatatggaat tacaaataag ttggtattat atgtaccaac atatagagaa gataaagcag
     8161 ataatagggc tattgataaa gcttattttg aaaaatgttt accaggatat acactgatta
     8221 ataaattaca tccatcaatt gaagattcag acattgatga cgtatcttca atcgacacgt
     8281 ctacattaat gctaatgtca gatataatta ttagcgacta tagttcgctg ccaatagaag
     8341 ctagcttgtt agatattcca actatatttt atgtgtatga tgaaggaaca tatgatcagg
     8401 tgagaggcct gaatcaattt tacaaagcaa taccggatag ctacaaagtg tatactgaag
     8461 aagatttaat aatgacgata caagaaaaag aacatctatt aagtccgtta tttaaagatt
     8521 ggcataagta taatactgat aaaagtttac atcagctcac agaatatata gataagatgg
     8581 tgacaaaatg aggtttacga taatcatacc tacatgtaat aatgaggcaa caattcgaca
     8641 attgttaata tctattgaga gtaaagaaca ctatagaatc ctttgtattg atggtggttc
     8701 tactgatcaa acaattccta tgattgaacg gttacaaaga gaactcaagc atatttcatt
     8761 aatacaatta caaaatgctt cgatagctac gtgtattaat aaaggtttga tggatatcaa
     8821 aatgacagat ccacatgata gtgacgcatt tatggtcata aaaccaacat caatcgtatt
     8881 gccaggtaaa ttagataggt taactgctgc tttcaaaaat aatgataata ttgatatggt
     8941 aatagggcag cgagcttaca attaccatgg tgaatggaaa ttgaaaagtg ctgatgagtt
     9001 tattaaagac aatcgaatcg ttacattaac ggaacaacca gatttgttat caatgatgtc
     9061 ttttgacgga aagttattca gtgctaaatt tgctgaatta cagtgtgacg aaactttagc
     9121 taacacatac aatcacgcaa tacttgtcaa ggcgatgcaa aaagctacgg atatacattt
     9181 agtttcacag atgattgtcg gagataacga tatagataca catgctacaa gtaacgaaga
     9241 agattttaat agatatatca cagaaattat gaaaataaga caacgagtca tggaaatgtt
     9301 actattacct gaacaaaggc tattatatag tgatatggtt gatcgtattt tattcaataa
     9361 ttcattaaaa tattatatga acgaacaccc agcagtaacg cacacgacaa ttcaactcgt
     9421 aaaagactat attatgtcta tgcagcattc tgattatgta tcgcaaaaca tgtttgacat
     9481 tataaataca gttgaattta ttggtgagaa ttgggataga gaaatatacg aattgtggcg
     9541 acaaacatta attcaagtgg gcattaatag gccgacttat aaaaaattct tgatacaact
     9601 taaagggaga aagtttgcac atcgaacaaa atcaatgtta aaacgataac gtgtacattg
     9661 atgaccataa actgcaatcc tatgatgtga caatatgagg aggataactt aatgaaacgt
     9721 gtaataacat atggcacata tgacttactt cactatggtc atatcgaatt gcttcgtcgt
     9781 gcaagagaga tgggcgatta tttaatagta gcattatcaa cagatgaatt taatcaaatt
     9841 aaacataaaa aatcttatta tgattatgaa caacgaaaaa tgatgcttga atcaatacgc
     9901 tatgtcgatt tagtcattcc agaaaagggc tggggacaaa aagaagacga tgtcgaaaaa
     9961 tttgatgtag atgtttttgt tatgggacat gactgggaag gtgaattcga cttcttaaag
    10021 gataaatgtg aagtcattta tttaaaacgt acagaaggca tttcgacgac taaaatcaaa
    10081 caagaattat atggtaaaga tgctaaataa attatataga actatcgata ctaaacgata
    10141 aattaactta ggttattata aaataaatat aaaacggaca agtttcgcag ctttataatg
    10201 tgcaacttgt ccgtttttag tatgttttat tttctttttc taaataaacg attgattatc
    10261 atatgaacaa taagtgctaa tccagcgaca aggcatgtac caccaatgat agtgaataat
    10321 ggatgttctt cccacatact tttagcaaca gtatttgcct tttgaataat tggctgatga
    10381 acttctacag ttggaggtcc ataatcttta ttaataaatt ctcttggata gtccgcgtgt
    10441 actttaccat cttcgactac aagtttataa tcttttttac taaaatcact tggtaaaaca
    10501 tcgtaaagat cattttcaac ataatatttc ttaccattta tcctttgctc acctttagac
    10561 aatattttta catatttata ctgatcaaat gagcgttcca ttaatgcatt ccccatcata
    10621 ttacgttgct tctcgccacc aaggttttta tagtctcctg cacccatgat aacttgatta
    10681 attctaaatt tacctcgttt ggtagtaatc gtatggttgt aatttgctgt atcacttgat
    10741 ccagttttta aaccatctgt acccggcaaa ctcatttttg caccttccaa tgaaaagttg
    10801 aatgtgtaat acgtaactgc atgcgttgtt ggtgctaact gctttgtaaa gtctaatatt
    10861 ttaggtgtct ctttaatcac gtgtaaatct aaaatggcat agtctctagc agtcgttaca
    10921 gtacgttctt ggtctttata ctttgttggt gcaaatgtac gtaatcttga attttcagca
    10981 cccgttggat tgacgaaatg tgtatttttc attccgatag ctttagcttt gttattcatt
    11041 aaatcaacga aatcgctggt gttttttgaa accttcttag ctaaaattaa tgccgcggca
    11101 ttactagaat tagatactgt aatttgtaat aggtctgcga ttgtccatac ttgtccagga
    11161 tatagtttcg tattactcaa ctcaggtagt gtagacataa tatattcttt gttcgtcatt
    11221 gtgactgtgt catcaagtga aagctgcccc ttatttacag cttccaatgt taagtacatt
    11281 gtcattaatt tagtcataga cgctggattc cacttagtat cgatattgta ttgatacagt
    11341 aattgtccag tttgacttac attaacagca ctcgtcggtt cgtatgcagc cgacaaacct
    11401 gcataaccat attgatttgc tgcttgtaca ggggttacgt cactgttagt agcttgtgca
    11461 tatggtgtca taatacttaa tgttaaacat aaaatgatga taatagatat taaatttttc
    11521 ataaagcgtt aatcttccct tttccaattc ttaaatattc cctaaaagca atggttattc
    11581 ctacttacgg aaatcattgc taattcactt caccttaatt aaattgttga aaataaagtt
    11641 ttctgcagtt aatttgaaaa ataatgcaaa tatattacgt gtgtagctaa aggtgttata
    11701 atgtttgtac gaagagcaaa cttactcaaa agcgattaat tttcatgttt taatataaag
    11761 actttgagaa gttattacaa aaaatgcaat agaaatattc tatcatataa atgttatgag
    11821 cggtattttg gggcaacact ttatttgatt tttaaagttt tgttgggaga aagtatatga
    11881 tagaaatgca tgtatctatc taaatgaatt aactataaat ttcaaacaga agaggtaaaa
    11941 ctatgaaacg agaaaatcca ttgtttttct tatttaaaaa actatcatgg ccagtgggtc
    12001 ttatcgttgc agctatcact atttcatcac tagggagctt aagtggacta ttagtgccac
    12061 tgtttactgg acgaattgta gataaatttt ccgtgagcca tatcaattgg aatctaatcg
    12121 cattatttgg tggtatcttt gtcatcaatg ctttattaag cggattaggt ttatatttat
    12181 taagtaaaat tggtgaaaag attatttatg cgatacgctc agttttatgg gagcatatca
    12241 tacaattaaa aatgccattc tttgacaaaa atgaaagtgg tcaattaatg agtcgattaa
    12301 ctgacgatac gaaagtgata aatgaattta tttcacaaaa gctacctaac ttattaccat
    12361 caatcgttac attagttggg tcactaatca tgttatttat tttagattgg aaaatgacat
    12421 tattaacatt tataacgata ccgatattcg ttttaattat gattcctcta ggtcgtatta
    12481 tgcaaaagat atcgacaagt acacaatctg aaattgcaaa cttcagtggt ttgttagggc
    12541 gtgtcctaac tgaaatgcgt cttgttaaaa tatcaaatac agagcgtctt gaattagata
    12601 atgcacataa aaatttgaat gaaatatata aattaggttt aaaacaggct aaaattgcgg
    12661 cagttgtaca accaatttca ggtatagtta tgttgctaac aattgcaatt attttaggtt
    12721 ttggtgcatt agaaattgcg actggtgcaa tcactgcagg tacattaatt gcaatgatat
    12781 tttatgttat tcagttatct atgcctttaa tcaatctttc cacgttagtt acagattata
    12841 aaaaggcagt cggtgcaagt agtagaatat acgaaatcat gcaagaacct attgaaccga
    12901 cagaagctct tgaagattct gaaaatgtat taattgatga cggtgtattg tcatttgaac
    12961 atgtagactt taaatatgat gtgaagaaaa tattagatga tgtgtcgttc caaatcccac
    13021 aaggtcaagt gagtgctttt gtaggccctt ctgggtctgg taaaagtacg atatttaatc
    13081 tgatagaacg tatgtatgaa attgagtcag gtgatattaa atatggcctt gaaagtgtct
    13141 atgatatccc gttatctaag tggcgacgca aaattggata tgttatgcaa tcaaattcga
    13201 tgatgagtgg tacaattaga gacaatattt tatacggaat taatcgtcat gtttcagatg
    13261 aagaacttat taattatgct aaattagcga actgtcatga ttttatcatg caatttgatg
    13321 aaggatatga cacgcttgta ggtgaacgag gattgaaact gtctggcgga caacgtcaac
    13381 gtattgatat tgctagaagt tttgttaaaa atcctgatat tttgttactt gatgaagcaa
    13441 cagctaatct cgatagtgaa agtgaattga aaattcaaga agctttagaa acattgatgg
    13501 aaggtagaac aacgattgtc attgcgcatc gtttgtctac aattaaaaaa gccggtcaaa
    13561 ttatattctt agacaaagga caggtaacag gtaaaggtac gcattcagaa ctgatggcat
    13621 cacatgcgaa gtataaaaac tttgtagtgt ctcaaaaatt aacagattaa ttttatatat
    13681 ataagtaagc ttggagcaaa tacacatata ccatcgagga aattaaagtg tggcacattg
    13741 atggatatag atgttaataa attgcttcaa gcttttgtct attttaaatc atttgagaag
    13801 ttacgacata ataattctta aattaatgaa atcgatattt taagaaaaaa atgctcatgg
    13861 tataatacaa gttataagca aacatacata tattaaatac tgtagccacg agtcataatt
    13921 cttcgtattt tacatagcaa tttaactgat tttagagtcc acggtacaga agtttgatat
    13981 ttcaatgttt ctaaattttt aaaaaattaa atcataggtg ggtgccaaat gtttttatta
    14041 atcaacatta ttggtctaat tgtatttctt ggtattgcgg tattattttc aagagatcgc
    14101 aaaaatatcc aatggcaatc aattgggatc ttagttgttt taaacctgtt tttagcatgg
    14161 ttctttattt attttgattg gggtcaaaaa gcagtaagag gagcagccaa tggtatcgct
    14221 tgggtagttc agtcagcgca tgctggtaca ggttttgcat ttgcaagttt gacaaatgtt
    14281 aaaatgatgg atatggctgt tgcagcctta ttcccaatat tattaatagt gccattattt
    14341 gatatcttaa tgtactttaa tattttaccg aaaattattg gaggtattgg ttggttacta
    14401 gctaaagtaa caagacaacc taaattcgag tcattctttg ggatagaaat gatgttctta
    14461 ggaaatactg aagcattagc cgtatcaagt gagcaactaa aacgtatgaa tgaaatgcgt
    14521 gtattaacaa tcgcaatgat gtcaatgagc tctgtatcgg gagctattgt aggtgcgtat
    14581 gtacaaatgg taccaggaga actggtacta acggcaattc cactaaatat cgttaacgcg
    14641 attattgtgt catgcttgtt gaatccagta agtgttgaag agaaagaaga tattatttac
    14701 agtcttaaaa acaatgaagt tgaacgtcaa ccattcttct cattccttgg agattctgta
    14761 ttagcagcag gtaaattagt attaatcatc atcgcatttg ttattagttt tgtagcgtta
    14821 gctgatctat ttgatcgttt tatcaatttg attacaggat tgatagcagg atggataggc
    14881 ataaaaggta gtttcggttt aaaccaaatt ttaggtgtgt ttatgtatcc atttgcgcta
    14941 ttactcggtt taccttatga tgaagcgtgg ttggtagcac aacaaatggc taagaaaatt
    15001 gttacaaatg aatttgttgt tatgggtgaa atttctaaag atattgcatc ttatacacca
    15061 caccatcgtg cggttattac aacattctta atttcatttg caaacttctc aacgattggt
    15121 atgattatcg gtacattgaa aggcattgtt gataaaaaga catcagactt tgtatctaaa
    15181 tatgtaccta tgatgctatt atcaggtatc ctagtttcat tattaacagc agctttcgtt
    15241 ggtttatttg catggtaata tgtcgaagag tgactatgat aatacatttt aactaataaa
    15301 tatgtccagg catgtcgtct attgatatag gtgagatgct tggacttttt tattattgat
    15361 ataaaggtat ttaaatattt ttaaagttac cgaaattgaa gcattataaa aaccaagtgc
    15421 acatggtaat acacttggct tttatgggaa atgaatatta ttgtacatat gacagtaagg
    15481 actaggtaca gtcatagtac ttcgagcaaa atttgttttg ttattataaa caacacaaag
    15541 gagataactt ctctattgaa gaagttaaaa acattatagc agacaatgaa atgaaagtaa
    15601 attaaaaatt cagaatattt ttaattatta tattgtgagt gatatttatt agggaaagct
    15661 attcttcata taaattagtt aaatagtaaa tctttgttag aactctttcc atagagttgt
    15721 gacagcattt ttgttgtgct actattttta tagtctaata aatatataaa ggggatggtt
    15781 tcgtgaataa aacggttaaa gatttaatac tagttgtctt aggttcattt atctttgctg
    15841 caggtgtaaa tgcatttatt atttctggta acttaggtga aggcggggtt acaggtttag
    15901 caattatttt atattatgcg tttcatattt caccagccat cactaacttc ttggtcaacg
    15961 cagtattgat tgccaaaggt tataaatttt tgagtaagag aagtatgtac ttaactattc
    16021 ttgtaacaat tcttatttca atatttttga gtttaacaga atcatggcaa gtagaaactg
    16081 gaaacagcat tgtgaatgcc atttttggtg gtgtaagcgt tggactagga atcggagtaa
    16141 ttatccttgc aggcggtaca acagcaggta caacaatttt ggcgagaatt gcaacgaaat
    16201 acctcgatgt aagcacgcca tatgctttgc ttttcttcga tatgatcgtt gttgcaattt
    16261 cacttacagt tattccactt gataaagtat tagtaacagt aatatcactt tatataggaa
    16321 caaaagtgat ggaatatgtc atagaaggtt taaacactaa aaaagctatg acgattattt
    16381 caactaatcc cgacaaactt gccaaagcaa tagacgagca aattggaaga ggtttaacca
    16441 ttttaaacgg acatggctat tatacgcgtg aagaaaaaga tgtcttatac gttgttattt
    16501 ctaaaacaca agtttcaaaa gcaaagcgat taattaaaca aatcgataaa gatgcattcc
    16561 tcgtaattca tgatgtaaga gatgtctatg gtaatggctt tcttgcagat gaataaataa
    16621 atggtatgag cacacatact taaatagaag tccacggaca agtttttgaa ctatgaagac
    16681 ttatctgtgg gcgtttttta ttttataaaa gtaatataca agacatgaca aatcgagcta
    16741 tccaatttaa aaagtaatgt tagtcaataa gattgaaaaa tgttataatg atgttcatga
    16801 taatcattat caattgggat gtctttgaaa attgataatt taaaaataga aattattttt
    16861 tataaacaga aagaatttta ttgaaagtag ggaaattatg aatcgtttgc atggacaaca
    16921 agttaaaatt ggttacgggg ataacacgat tataaataaa ttagatgttg aaataccaga
    16981 tggcaaagtg acgtcaatca ttggtcctaa cggctgcggg aaatctactt tgctaaaggc
    17041 attgtcacgt ttattggcag ttaaagaagg cgaagtattt ttagatggtg aaaatattca
    17101 tacacaatct acgaaagaga ttgcaaaaaa aatagccatt ttacctcaat cacctgaagt
    17161 agcagatggc ttaactgttg gggaattagt ttcatatggt cgttttccac atcaaaaagg
    17221 atttggtaga ttaactgctg aggataagaa agaaattgat tgggcaatgg aagttacagg
    17281 aactgataca ttccgacacc gttcaatcaa tgatttaagt ggtggtcaaa gacaacgtgt
    17341 ttggattgca atggcattag cacaaagaac tgatattatc tttttagacg aaccaacaac
    17401 atatttagat atctgtcatc aattagaaat actagaatta gttcagaagc taaatcagga
    17461 acaaggttgt acaattgtca tggttcttca tgatatcaac caagcgattc gtttctcaga
    17521 tcatcttatt gcgatgaaag aaggggatat catcgctaca ggttcaacag aagacgtatt
    17581 aacacaggaa atattagaaa aagtttttaa tattgatgtt gttttaagta aagatcctaa
    17641 aactggaaaa cctttactgg taacttatga cttatgtcgc agagcttatt cttaattaag
    17701 taagttaata tgataaaaag gacaattaac atgacaaata gagagaaccc aacgccattg
    17761 aagtttttat cctatattat aggtttaagt atgatactac taatcacact atttatttct
    17821 acattaatag gtgacgccaa aattcaagcc tctacaatta tagaggctat ttttaattat
    17881 aatcctagca atcaacagca aaacatcatc aatgagatta ggattcccag aaatatagca
    17941 gcagtaattg taggtatggc gcttgcagtt tctggtgcga ttatacaagg tgttactcgt
    18001 aatggtcttg ctgatccggc gctcataggt ttaaattcag gtgcttcatt tgctttagca
    18061 ttaacatatg cagttttacc aaacacttca tttttaatat tgatgtttgc tggattttta
    18121 ggtgctattc taggaggtgc tattgtatta atgataggcc gatctagacg tgatggattt
    18181 aatccgatgc gtattatttt agcgggtgca gcagtaagtg ctatgttaac agcgctaagt
    18241 caaggtattg cattagcttt tagactaaat caaacagtaa cattttggac tgctggaggc
    18301 gtttcaggca caacatggtc acaccttaag tgggcaattc cattaattgg tattgcgtta
    18361 ttcattatat taacaattag taaacaactt accattttaa atcttggtga atcattagct
    18421 aaaggtttag gtcaaaatgt aacaatgatc agaggcatat gtttaattat tgctatgatt
    18481 ctagcaggta ttgcagttgc tatcgctgga caagttgcat ttgtaggttt gatggtacct
    18541 catatagcaa gatttttaat tggaactgat tatgctaaaa ttctaccatt aacagccttg
    18601 ttaggtggga tactcgtgct tgttgccgat gtgatagcac gatatttagg agaagcgcct
    18661 gttggtgcaa tcatttcatt tatcggtgtt ccttactttt tatatttagt taaaaaagga
    18721 ggacgctcaa tatgattagt tcaaataata aacgcagaca attgatagca ctggctgttt
    18781 ttagcattct actatttcta ggttgtactt ggagtattac ctcaggtgaa tacaacatac
    18841 ctgttgaaag atttttcaaa actttaattg gacaaggtga tgccattgat aagttaatct
    18901 tattagattt caggttacct cggatgatga ttactatttt ggctggcgca gcgcttagta
    18961 ttagtggtgc aatagtgcaa agtgtcacaa aaaatccaat agctgaacca ggtatattag
    19021 gtattaacgc aggtggcgga tttgcaatcg cattatttat tgcaattggt aaaattaatg
    19081 ctgacaactt tgtttatgta ctgccgttaa taagtatact aggtggtatc accactgcat
    19141 tgattatttt tattttcagt tttaataaaa atgaaggtgt tacacctgcg agtatggtat
    19201 taataggtgt aggtttacaa acagcattat atggtggctc aattacaatt atgtcaaaat
    19261 ttgatgataa gcaatctgat ttcatcgctg cttggtttgc aggtaatatt tggggtgacg
    19321 aatggccatt tgtcattgca tttttaccgt gggtgttgat tattattcct tacttactat
    19381 ttaaatcgaa tacactaaat attattcata cgggtgataa tattgcacga ggtctaggtg
    19441 taaggttaag cagagaacgt ttaatattat tctttatcgc agtgatgtta tcatctgctg
    19501 ctgtagcagt agcaggttca atttcgttta tcggattaat gggtccgcat attgccaaac
    19561 gtatcgttgg accacgtcac cagttgtttt taccaattgc cattttagta ggggcatgtt
    19621 tacttgttat agctgataca attggcaaaa ttgtattaca accaggtggg gttccagcag
    19681 gtattgtcgt agcaattatt ggtgcaccgt atttcttata tttaatgtac aaaacgaaaa
    19741 atgtatagtg tcaatggaca caacttattg ctatgaaagg cactttatta taaggctttt
    19801 catagcattt tttatttaat gagccactca agactattta ttttttcaat aatgaaccat
    19861 taagttatca agaggatctt atcaaaaata tatttgataa cggtatcagg ttaattcttt
    19921 atgatagcgc attcatttat tctgttttat actatgactg ataataccaa ggaggtacaa
    19981 catgatgaaa aagttaatca ataaaaaaga aacattttta actgatatgc ttgaaggatt
    20041 gttaattgcg cacccagagt tagatctgat tgctaataca gttattgtaa aaaaagctaa
    20101 gaaagaacat ggtgtagcaa tagtctctgg aggtggaagc ggacatgaac ctgcgcatgc
    20161 cggttttgtt gcagaaggta tgctagatgc agcggtttgt ggcgaagtat ttacatcacc
    20221 tacacctgat aaaatattag aagctattaa agcagtagat actggtgatg gtgtattact
    20281 agttgtaaaa aactatgcag gtgacgtgat gaatttcgaa atggcacaag agcttgcaga
    20341 aatggaaggt ataaatgttc aaactgttat tgttcgtgac gacattgctg tgacaaacga
    20401 agtacaacgt cgtggtgttg caggaacagt gtttgttcat aagcttgccg gttatcttgc
    20461 tgaaaaaggt tattcattaa cagagataaa atcgcgtgta gaagcgttgt tacctgaaat
    20521 taaaagtatt ggtatggcaa ttgagccacc gcttgttcca actactggaa aatatggctt
    20581 tgatattgaa gacgacaaaa tggaaatcgg tattggtata catggtgaaa aaggtattca
    20641 tagggaagaa gtaaaggata ttgatcatat tgttggaaca ttgttagacg aattgtataa
    20701 agaagttact gccaatgatg tcatattaat ggtaaatggt atgggtggta cgccgttatc
    20761 tgaattaaat atcgtaacta aatatattca acaaaattta gctgcaagaa cggttaatgt
    20821 tgctaaatgg tttgttggtg attatatgac atctttagac atgcaaggtt tttctataac
    20881 tatcgtgcct aataaaccag aatatttgga agcattttta gcaccaacaa caagtcaata
    20941 ctttaaataa gaagtgaata tgaataaata tacatttatg aggtggcaca aatgaaagtg
    21001 aatgatatga aagcacgttt attaaattta gaagaaacgt ttaaaaaaca tgaatctgaa
    21061 ttaactgaat tagatcgagc aattggtgat ggtgaccacg gggttaacat ggttcgtggg
    21121 tttagtagtc ttaaagacaa acttgatgat agctcaatgc aatcattgtt caaatcaact
    21181 ggtatggcat tgatgtcaaa tgttgggggt gcatcaggac cactgtatgg ctttagcttt
    21241 gttaaaatgt ctgcagtcac caaagatgat atggataatc aagatttcat tacactaatt
    21301 caggcatttg ccgaagcggt tgaatcacgt ggtaaagtta ctttaaatga aaagacaatg
    21361 tatgatgtag tagcgcgagc agcagagaag cttaaaaatg gtgaaacttt aacattcaat
    21421 gatttacagc aattagcaga taatacaaaa gatatggtag caacgaaagg tagagctgca
    21481 tattttggag aagaatcaaa aggttatatt gatccaggtg ctcaaagtat ggtttatatt
    21541 ttaaacgctt tgattggaga tgaagataat gcctaaaatt atacttgtta gccacagtaa
    21601 agaaattgca agtggtacaa aatctttgtt aaagcaaatg gcaggtgacg ttgatattat
    21661 accaatcggg ggattaccag atggttcaat tggaacttca tttgatatca tccaagaagt
    21721 tttgactaaa ttagaggatg atgcattgtg tttttacgat attggatctt cagaaatgaa
    21781 tgtagatatg gcaattgaaa tgtatgatgg taatcatcgt gtgttaaaag ttgatgcacc
    21841 aattgttgaa ggcagtttta tcgcagcagt aaagctatca atcggcggtt caattgatga
    21901 tgcattagca gaaatcaaac aatcatttta gttaaaattt actaataatg aaaaatgtaa
    21961 acctttttca aatgaaactt tataaaaaat atgatagtat atatgtaaat gtttaataaa
    22021 atctggagaa ataggaggac attgccatgc aacaccttat aaaaaaacat gtattgaatg
    22081 gcgagtttga tttagtacga caattgatgt ccgaaacaga ttttatggaa tttgaagaag
    22141 catatatttc aagtgcgcat gaagtagaaa gtatgatgtt ttatacatgt attttagata
    22201 tgattaagta cgaagaatca tctgaaatgc atgacttagc atttttattg cttgtgtatc
    22261 cactaagtga atatgaaggt gctttggatt ctgcttatta tcatgcagac gcttccataa
    22321 aacttactga cggcaaagaa gttaaaagtt tgttacaaat gttattattg catgcgatac
    22381 caacacctgt tatttcagat aagaaggctt ttgatatcgc caagcaaatt ttaaaattag
    22441 atcctaataa taatgttgct cgtaacgtct taaaagacac tgccaaacgt atggacaacg
    22501 ttgttgttga tataaatgaa ttacaccaac gtaatgcacg ttaattacat ttcaattata
    22561 ttagcttaat aatagtttta acatttggtt gggttgggca tatgttccag ccttttttaa
    22621 tacttaaaaa ctaacgaagt atacttgtgt gcacaaatgg tttttataca acattttata
    22681 aatttataca ttttaataaa gaacatacga tagatggttt aaaccttgtt aactgagaaa
    22741 ttttgatatg tattcttcga aatttaacta aatatacgaa attcaagaag cacaataatt
    22801 aatcattttt cctatacaaa agttcgtatg actgcattat aaaagcataa atttataatt
    22861 tttttaaatg tcattgaacg tgataatgtg aatggattga gcaattttga aaaagtgaaa
    22921 aataacctat gcggcttgca attaattttc agtacgttat aatgcacact gtgcaaaatt
    22981 aaggaggtct attattcaca tgatgatgaa taaagaagca acaaaaattg gatttgccta
    23041 cgtcggcatt gtagtgggcg caggattttc aactggacaa gaagttatgc aatttttcac
    23101 taaatatggc ttgtgggctt atttaggtgt tattatatct ggttttattt tagcttttat
    23161 tgggcgccaa gtagcaaaaa ttggtactgc ctttgaagcg acaaatcatg aatcaacatt
    23221 acaatacgta ttcggtgaaa agtttagtaa agtctttgat tatattttaa tcttcttctt
    23281 atttggtata gctgtaacca tgatagctgg tgcaggcgca acatttgaag aaagttataa
    23341 catacctaca tggctaggtg ctttaattat gacattagcg atttatatta cgttgctatt
    23401 agactttaat aaaatagtac gtgcactagg tatcgttaca ccatttttaa ttgttttagt
    23461 tgtattaatc gctggcgttt atttatttaa aggtcatgtt tcattagcag aagttaacca
    23521 agtagtgcct gaagcaagta tttggaaggg aatctggttt ggtacaatat atggtggatt
    23581 agctttttct gtaggtttta gtaccatcgt agcaatcggt ggggatactg aaaagcgtac
    23641 agtgtcaggt gcaggcgcga tgtatggtgg tattatctat actgtattac tagcattgat
    23701 caactttgca ttgcaaagtg aatatccaac tattaaaaat gcctcaattc ctacattgac
    23761 gttagcaaat aatatccatc ctttaatagc aacagtgtta tctgttatta tgctggcggt
    23821 tatgtataat actattctag gactaatgta ttcatttgca gcacgtttta cagaaccata
    23881 cagtaaaaat tatcatatct ttattattat aatgatggta gcaggttatt tattaagttt
    23941 cgtaggattt gctgaattaa ttaataagtt atatacaatt atgggatatg taggcttatt
    24001 tattgtagta gctgtaatta ttaaatattt caaacgtaaa aatgcggata aaaaacatat
    24061 tgcttaatat catatgaggg atatccgaaa ctttacaatt gaatcacttt gttttaacct
    24121 taaaagcaat tcgtctctac tcttatcggg cgaattgctt tttatattta ttcagtctat
    24181 taatatgagc gtctaacaaa tagagaggta cgatgtaatg aataaagata ataaatggac
    24241 gatgataact gcgcttttta taactgtaat cagtgtattg ttagcatttc atctgaaaca
    24301 acattatgac caaattacaa atgagaacca tgctaataaa gacaaaatta atattaaaaa
    24361 taaaaatgtg cgcatttatc aaaaccttac atacaataga gttttcccta acagtaaatt
    24421 agatattatt acacctgttg atatgtcttc taatgccaaa ctgccagtta ttttttggat
    24481 gcacggtggt ggttatattg cgggtgataa gcagtataaa aacccattat tagcgaaaat
    24541 tgctgaacaa gggtacattg ttgtgaatgt aaattatgca ttggcgccac aatataaata
    24601 tcccacacca ttaattcaaa tgaatcaagc aactcaattc attaaagaaa ataaaatgaa
    24661 tttacctatt gattttaatc aagtaattat tggcggtgat tctgcaggtg ctcaattagc
    24721 tagccaattt acggcaatac agacgaatga tcgcttaaga gaagccatga aatttgatca
    24781 gtcattcaaa ccatcgcaaa ttaaaggtgc tatactattt ggtggttttt ataatatgca
    24841 aacagttaga gaaactgagt ttccaagaat acagttattt atgaaaagtt atactggcga
    24901 agaagattgg gaaaagagtt ttaaaaacat ttcacaaatg tcgacagtaa aacaatcgac
    24961 aaaaaattat ccaccaacat ttttatctgt tggagatagc gatccattcg aaagtcaaaa
    25021 tatagaattc agtaagaaat tacaagaatt gaatgtacca gtagatactt tgttttatga
    25081 tggtacgcat catttacatc atcagtatca atttcacctt aataaacctg aatcgataga
    25141 taatatcaaa aaagtgttac ttttcttaag tcgtaataca tcctctagtg gtattcaaac
    25201 tgaagagaaa ccacaaatag aaaatccgag taatgaatta ccgttaaatc ctttaaacta
    25261 atgataaaca gtagtaattt attgcttaag caacatttaa gattttcaaa ttaaaaacga
    25321 agaatttaaa acatgtggtg ctaatgtgta agagtctgag atataataaa ttgttagcgg
    25381 ttctttatca tttctatctc actctattat gatgtgacat ttattttaca aactaatttg
    25441 ttttgaacct gaaaataact ttttacaact aaattgtcga aaaacagtgt gtacatgata
    25501 taataaattt ataaattgaa aagaattcaa aagaaaaatt aaaatataga ttgagcacga
    25561 agtgatttga aataaggttg tgaaagggaa tgacaaggtc agcattaaaa ccatttaaaa
    25621 ataaacgcgt tatggttact ggacgtatac aacgtgtttt gtttaaaaat tatttagata
    25681 gacatagcac atttaagccg aatgtaagga tattattaaa agatgtattt gtttcaggtg
    25741 tatcaataga tcatttatgg ttatatgaga caaataaata ctatgcattg gcaatggaac
    25801 ttattcatca acgagtaaaa tttagtgcga atgttgtacc gtattacaaa ataaatagaa
    25861 ataataattt attcgtacaa gattatggaa ttaagcgtaa aggtaggtta attactgaag
    25921 aagcttacaa tcaaaacaat cagtatcagg ataagatata tgaaaaatta ccggatatag
    25981 attttagact cgaagatttt tatagtaagg aaaactaaac aacatatata aagcaccact
    26041 aatcgtagtt gatagttaat caattacaca ttagtggtgt tttattttga atttaaattt
    26101 gataaatgaa taagagtgta cgtatttatt tgagattaga taacattgat aagtatgagt
    26161 aatgtattag gtaaccatta aattcgttgt tatacgattc tgatacaaga ttatgataaa
    26221 atagctttaa ttaagaattt tagccatcat atagtcatca taatatttac catcgataaa
    26281 taacttatct tttaaaacgc cttcgatttg aaaatcggca cttttaaaaa gctcgagggc
    26341 aggttggtta ttgagtggta catttgcttc aattcggtgt atttgattgt ttaaacacca
    26401 agccataatg gcatcaagaa gtgcttggcc aattccacga tgttgatata atttctttac
    26461 acctaaatca attttagcaa catgtttaat gcgttgaaat ggtgtcgtat taacaaaggc
    26521 aaagccaacg agttgttcat cactttcagc aacgaagatg actttatgcg gagaagtgat
    26581 atattcttct aattgtttac tagccgatgt gacgctagga tcatattctc ctggtgtgta
    26641 gaacatatac ggagattcgt cgtatatgtt cgctaacatt gaaatgaaat tttctacatc
    26701 tttgatacta actctacgta taatatgggc cataaaaagc ctccagtatt ttgaaatttt
    26761 aaaacatttg ctactattat aatatatatg taactaaaag gtggagtaat atgttttttg
    26821 gttacagttt aaaggagtat tttagatgaa acctaaagtt ttattagcag gtggaacagg
    26881 atatattggt aagtatttaa gtgaagtgat tgaaaatgat gctgaacttt ttgctatatc
    26941 aaaatatcca gacaataaaa aaacagatga tgttgaaatg acttggattc agtgtgatat
    27001 atttcattac gaacaggttg ttgcagcaat gaatcaaata gatattgctg tattctttat
    27061 cgacccaaca aagaattctg ccaaaataac acaatcatca gcaagagatt taacattaat
    27121 cgcagcagat aattttggtc gagcagcggc tatcaatcaa gtaaaaaaag taatctacat
    27181 acctgggagt cgttatgata atgaaacaat tgaacgccta ggtgcatatg gtacacctgt
    27241 agaaacaaca aatttagttt ttaaacgttc tttagttaat gtagaattac aagtttcaaa
    27301 gtatgatgat gttagatcaa cgatgaaggt agttttacca aagggatgga cattaaagaa
    27361 cgttgtaaac cattttattg catggatggg ttacactaaa ggaacttttg tgaaaacaga
    27421 aaaatcacat gatcaattta agatatatat taagaataag gtgcgaccgc tcgcagtatt
    27481 taaaatagaa gaaacagctg atggaataat aactttaatt ttattgagtg gaagtttagt
    27541 gaaaaaatat acagttaatc aagggaagtt agaatttaga ttaatcaaag agtcggcagt
    27601 cgtttatata catctatacg attatatccc tcgattattt tggccgattt attactttat
    27661 acaagcacca atgcaaaaaa tgatgattca tggctttgaa gttgactgcc ggattaaaga
    27721 ttttcaaagt cgattaaaat caggagaaaa tatgaaatat actaaatgat attgggtgat
    27781 atggatgcaa atactactag tagaagatga caatactttg tttcaagaat tgaaaaaaga
    27841 attagaacaa tgggatttta atgttgctgg tattgaagat ttcggcaaag taatggatac
    27901 atttgaaagt tttaatcctg aaattgttat attggatgtt caattaccta aatatgatgg
    27961 gttttattgg tgcagaaaaa tgagagaagt ttccaacgta ccaatattat ttttatcatc
    28021 tcgtgataat ccaatggatc aagtgatgag tatggaactt ggcgcagatg attatatgca
    28081 aaaaccgttc tataccaatg tattaattgc taaattacaa gcgatttatc gtcgtgtcta
    28141 tgagtttaca gctgaagaaa aacgtacatt gacttggcaa gatgctgtcg ttgatctatc
    28201 aaaagatagt atacaaaaag gtgatcagac gattttcctg tccaaaacag aaatgattat
    28261 attagaaatt cttattacca aaaaaaatca aatcgtttcg agagatacaa ttatcactgc
    28321 attatgggat gatgaagcat ttgttagtga taatacgtta acagtaaatg tgaatcgttt
    28381 acgaaaaaaa ttatctgaaa ttagtatgga tagtgcaatc gaaacaaaag taggaaaagg
    28441 atatatggct catgaataat ttgaaatggg tagcttattt tttgaaatct cgcatgaact
    28501 ggatattttg gatattgttt ttaaacttcc ttatgttagg cattagtcta atcgattatg
    28561 attttccaat agacagttta ttttatattg tttctttgaa tttaagttta acaatgattt
    28621 ttcttttatt gacatatttt aaagaagtaa aattatataa gcattttgac aaagataaag
    28681 aaatagaaga aattaaacat aaagatttag cggaaacgcc atttcaacgt catacagttg
    28741 attatttata tcgtcaaatc tcagcgcaca aagaaaaggt tgttgagcaa cagttgcaat
    28801 tgaacatgca tgaacaaacc attacagaat ttgtgcacga cataaaaaca cctgtgacag
    28861 ctatgaaatt attaattgat caagaaaaaa atcaagaaag aaaacaagca ttactatatg
    28921 aatggtctcg tataaactcg atgctagata cacagctgta tattactaga ttagaatctc
    28981 aacgtaaaga tatgtatttt gattacgtgt cacttaaacg catggtcatt gatgaaatac
    29041 aattaacaag acatattagt caggttaaag gtattggttt tgatgttgac tttaaagtgg
    29101 atgattatgt ttatacagat ataaaatggt gtcgtatgat tattagacaa attttgtcaa
    29161 acgcattgaa atatagtgag aattttaata ttgaaattgg gacagaatta aatgatcaac
    29221 atgtttcgtt atatattaaa gactatggca gaggtattag taaaaaagat atgccgcgaa
    29281 tatttgaacg aggatttacg tcaacggcta acagaaatga aacgacgtct tcaggtatgg
    29341 gtctatattt agtaaatagt gtaaaggatc aattaggtat tcacctgcaa gtcacgtcga
    29401 ctgttggtaa ggggacaact gtcagattga ttttcccatt acaaaatgaa attgttgaac
    29461 gcatgtcgga agtgacaaat ttgtcatttt aaacatgcgt tttgttactt agaattgata
    29521 catcaatgca gcttcaacgt tataataaga tagatgttag tcatatgtta aattgaagat
    29581 acaagtgcca aagcctaaag gaaatgaagt taagataaat tataggagtg ttaaagtggc
    29641 aattttagaa gtaaaacaat taacaaaaat atatggaact aaaaaaatgg cacaagaagt
    29701 gttgcgagat atcaatatgt ctattgaaga aggcgagttt attgctatta tgggtccctc
    29761 tggatctggg aaaacgacat tattaaatgt tttaagttca attgattata tttcacaagg
    29821 ttctattaca ttaaaaggaa aaaaattaga aaagctttca aacaaggaat tatctgatat
    29881 acgcaagcat gatattggtt ttatttttca agagtataat ttactgcata cattgactgt
    29941 taaagaaaac ataatgttac cactaacggt acagaagtta gataaagaac atatgttaaa
    30001 tcgttatgaa aaagtagcag aagcattaaa tatattggat attagtgata aatatccctc
    30061 tgaattgtct ggtggacaaa ggcaacgaac atcagctgcc agagcattta taacattgcc
    30121 ttctattata tttgctgacg aaccaacagg tgcattggat tctaaaagta ctcaagattt
    30181 attaaaacga ttaacaagaa tgaatgaagc atttaagtct acaattatta tggtaacgca
    30241 tgatcctgtt gcagcaagct atgcaaatcg agtagtgatg ctaaaagatg gtcaaatttt
    30301 cactgaatta taccaagggg atgacgataa acataccttt ttcaaagaaa taatacgtgt
    30361 acaaagtgtt ttaggtggcg ttaattatga cctttaacga gataatattt aaaaatttcc
    30421 gtcaaaattt atcacattat gccatctatc ttttttcgtt aattacgagt gtagtattgt
    30481 attttagctt tgtagcatta aaatacgctc ataaactaaa catgacagag tcatatccaa
    30541 ttataaagga aggctcacaa gtcggaagct actttctatt tttcatcata attgcatttt
    30601 tgttatatgc caatgtgtta tttattaaac gacgaagtta tgagcttgca ttatatcaaa
    30661 cattaggttt atctaaattc aacattattt atatactaat gctcgaacaa ttactaatat
    30721 ttataattac ggcaatatta ggtattatta ttggtatttt tggttcgaaa ctgttattaa
    30781 tgattgtctt tacattatta ggaattaaag aaaaggttcc aattattttt agtttgaggg
    30841 cggtatttga aacattaatg ttaatcggtg tcgcttattt tttaacatct gctcaaaatt
    30901 ttatattagt gttcaaacaa tctatttcac agatgtcaaa gaataaccag gttaaagaaa
    30961 caaatcataa taaaattaca tttgaagagg ttgttttagg catcttaggt atagtattga
    31021 ttaccacagg atactatcta tctttgaaca ttgttcaata ttatgattct atcggtacac
    31081 ttatgtttat tttattgtca actgtgattg gggcatactt attttttaaa agctctgttt
    31141 ctctagtttt taaaatggtg aagaagttta gaaaaggtgt tataagtgta aatgatgtca
    31201 tgttctcatc atctattatg tatcgtatta agaaaaatgc tttttcactt acggtcatgg
    31261 caatcatttc agcgattact gtttcagttc tttgctttgc tgctataagt agagcgtcct
    31321 tatcaagtga aataaaatat actgcaccac acgacgttac aattaaagac caacaaaaag
    31381 ctaatcaatt agcaagtgaa ttaaacaatc aaaaaattcc tcatttttat aattataaag
    31441 aagtaattca tacgaaattg tataaagata atttatttga tgtaaaagcg aaagaaccat
    31501 acaatgtaac aattactagt gataaataca tccctaatac tgatttgaaa cgtgggcaag
    31561 ctgatttatt tgtagcggaa ggttctatca aagatttagt gaaacataag aagcatggta
    31621 aggcaattat aggaacgaaa aaacatcatg ttaatattaa gttacgtaaa gatattaata
    31681 aaatctattt tatgacagat gttgatttag gtggaccaac gtttgtctta aatgacaaag
    31741 actatcaaga aataagaaag tatacaaagg caaagcatat cgtctctcaa tttggattcg
    31801 atttgaaaca taaaaaagat gctttagcat tagaaaaagc gaaaaataaa gttgataaat
    31861 ctattgaaac aagaagtgaa gcgataagct caatatcaag tttaaccgga atattattat
    31921 ttgtaacatc atttttaggt attacattct tgattgctgt atgttgcatt atatacataa
    31981 agcaaataga tgaaaccgaa gatgagttag agaattatag tattttgaga aagcttggat
    32041 ttacacaaaa agatatggca aggggactaa agtttaaaat tatgtttaat tttgggttac
    32101 ctttagttat tgcactatca catgcatatt ttacatcatt agcatatatg aaattaatgg
    32161 gtacaacgaa tcaaataccg gttttcatag taatgggatt atacatttgt atgtatgctg
    32221 tttttgcagt gacggcttat aatcattcca agcgaacaat tagacattcc atataaaata
    32281 tacagatggc tttcagtaga gtagtggatt cggattcacg aactatactg gaagcttttt
    32341 attataaatg aagagaagtt atatttttag catgtatagt tgaatactgg gttaaaatac
    32401 catattaata atgaagtaaa ggtatgagtg attatgaaag tgttttgaat gaaatatatt
    32461 taattggtga tgcttttaat tgaaaagatt aacaggattc aactttgtaa attgtattaa
    32521 atgtgagaaa ataaaagtat attcattgag agatatatga gtcaatgatc gttttaaaca
    32581 agataagtgt attttaatat gtaaaagtta tgtaataaat attgtatcgt tgcaaatttc
    32641 ccaactatat ccatttacaa tttttagagt tgtatagata aaaaattgcc tatacaataa
    32701 ctttttcaaa acaaat
//
LOCUS       DALAAR010000027        32420 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000027 DALAAR010000000
VERSION     DALAAR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.027"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            111..923
                     /gene="lpl10"
                     /locus_tag="O2458_001287"
     CDS             111..923
                     /gene="lpl10"
                     /locus_tag="O2458_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCZ3887154.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            913..2241
                     /locus_tag="O2458_001288"
     CDS             913..2241
                     /locus_tag="O2458_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887155.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2238..2561
                     /locus_tag="O2458_001289"
     CDS             2238..2561
                     /locus_tag="O2458_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887156.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2580..2894
                     /locus_tag="O2458_001290"
     CDS             2580..2894
                     /locus_tag="O2458_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887157.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3184..3444)
                     /locus_tag="O2458_001291"
     CDS             complement(3184..3444)
                     /locus_tag="O2458_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887158.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3563..4765
                     /locus_tag="O2458_001292"
     CDS             3563..4765
                     /locus_tag="O2458_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ3887159.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5424..5486)
                     /locus_tag="O2458_001293"
     CDS             complement(5424..5486)
                     /locus_tag="O2458_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCZ3887160.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5550..5618)
                     /locus_tag="O2458_001294"
     CDS             complement(5550..5618)
                     /locus_tag="O2458_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCZ3887161.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5670..5735)
                     /locus_tag="O2458_001295"
     CDS             complement(5670..5735)
                     /locus_tag="O2458_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCZ3887162.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5767..5832)
                     /locus_tag="O2458_001296"
     CDS             complement(5767..5832)
                     /locus_tag="O2458_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCZ3887163.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6353..7837
                     /locus_tag="O2458_001297"
     CDS             6353..7837
                     /locus_tag="O2458_001297"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCZ3887164.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7850..10555
                     /locus_tag="O2458_001298"
     CDS             7850..10555
                     /locus_tag="O2458_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCZ3887165.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10717..11079
                     /locus_tag="O2458_001299"
     CDS             10717..11079
                     /locus_tag="O2458_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ3887166.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11308..11724)
                     /locus_tag="O2458_001300"
     CDS             complement(11308..11724)
                     /locus_tag="O2458_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887167.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11762..12436
                     /locus_tag="O2458_001301"
     CDS             11762..12436
                     /locus_tag="O2458_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ3887168.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            12473..13207
                     /locus_tag="O2458_001302"
     CDS             12473..13207
                     /locus_tag="O2458_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ3887169.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            13385..13498
                     /locus_tag="O2458_001303"
     CDS             13385..13498
                     /locus_tag="O2458_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887170.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            13663..15000
                     /locus_tag="O2458_001304"
     CDS             13663..15000
                     /locus_tag="O2458_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCZ3887171.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15217..16131
                     /locus_tag="O2458_001305"
     CDS             15217..16131
                     /locus_tag="O2458_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCZ3887172.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            16115..17257
                     /locus_tag="O2458_001306"
     CDS             16115..17257
                     /locus_tag="O2458_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCZ3887173.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            17551..18576
                     /locus_tag="O2458_001307"
     CDS             17551..18576
                     /locus_tag="O2458_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887174.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            18580..19239
                     /locus_tag="O2458_001308"
     CDS             18580..19239
                     /locus_tag="O2458_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887175.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19276..20118
                     /gene="gmpC"
                     /locus_tag="O2458_001309"
     CDS             19276..20118
                     /gene="gmpC"
                     /locus_tag="O2458_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCZ3887176.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            20450..21454
                     /gene="aaa"
                     /locus_tag="O2458_001310"
     CDS             20450..21454
                     /gene="aaa"
                     /locus_tag="O2458_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCZ3887177.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21639..21908)
                     /locus_tag="O2458_001311"
     CDS             complement(21639..21908)
                     /locus_tag="O2458_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887178.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            22057..22452
                     /locus_tag="O2458_001312"
     CDS             22057..22452
                     /locus_tag="O2458_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ3887179.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            22442..22927
                     /locus_tag="O2458_001313"
     CDS             22442..22927
                     /locus_tag="O2458_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887180.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(23096..23878)
                     /locus_tag="O2458_001314"
     CDS             complement(23096..23878)
                     /locus_tag="O2458_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ3887181.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(23875..24993)
                     /locus_tag="O2458_001315"
     CDS             complement(23875..24993)
                     /locus_tag="O2458_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ3887182.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(25108..25992)
                     /locus_tag="O2458_001316"
     CDS             complement(25108..25992)
                     /locus_tag="O2458_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3887183.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            26173..30672
                     /gene="gltB"
                     /locus_tag="O2458_001317"
     CDS             26173..30672
                     /gene="gltB"
                     /locus_tag="O2458_001317"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCZ3887184.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            30690..32153
                     /locus_tag="O2458_001318"
     CDS             30690..32153
                     /locus_tag="O2458_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCZ3887185.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
BASE COUNT        11034 a         4733 c         6522 g        10131 t
ORIGIN      
        1 ttgaataagt tagttttgtt tgataacata aaagttgaat aatcactatt ttaataagtt
       61 gtatgaaata ttcaaatgtt tataaaatta aaacagagag atgtgaaatg atggagtata
      121 taaaaaaaat tgctttgtac atgagtgtat tacttttaat catttttatt gggggatgtg
      181 gaaatatgaa agatgaacag aaaaaagagg aacaaacgaa taaaacagat tcaaaagaag
      241 aacaaatcaa aaagagtttt gcgaaaacgt tagatatgta tccaattaag aatctcgagg
      301 atttatacga caaagaagga tatcgagatg gtgagtttaa aaaaggcgat aaaggtacgt
      361 ggactatact tacaggtttt tcaaaaagta acaaaccagg agtattagat gatgaaggca
      421 tggtgttata tcttaataga aatgccaaaa aggcaacagg ttattatttt gtaaataaag
      481 tttatgatga tattagcaaa aatcataatg agaaaaaata tcgtgttgaa ctaaaaaata
      541 ataagattgt tcttttggat aatgtagaag acaaaaaact taaacaaaaa attgaaaatt
      601 ttaaattttt tagtcagtat gctgatttta aagatttaaa aaattatcaa gatggaaata
      661 taacaactaa tgaaaacgta ccgagctatg aagcacaata taaaatgaac aatagtgata
      721 aaaatgtaaa aaaacttaga gaaatttatc caattacaac taataactct ccaaatctaa
      781 aattatatat agatggtgat ataaaaggaa gctcagtagg atataaaaaa atagaatata
      841 aattttcaaa agataaaggt caagagacaa cattaagaga ttatttgaat tttggaccgt
      901 ctgaaggtga gaatgttgag taggaagtat aaaatagatt taaaagcaat taatcaaaat
      961 actgagtcta caagtgctat ttcgaaagcg tcatacgaag ttgagaatgc taataataat
     1021 ggtctatcaa aaagagacgt gattaatcaa tttaatgatt tgaaaaaaat gaaaaaattc
     1081 ccttctaatc ttgaatatgt tgatagttac actgattctc ttactggagt aacaacttct
     1141 gcttttttaa ataaagatac aggcaaagta actctcggga tgactgggac taatttacaa
     1201 gacgaagcct ttaaaaagtt aaaagaaggt gaattttcaa gacaaaatgt taccaatgct
     1261 ttggaaacag ttaaagatgg atatgcagat cttaaaatat tatattctcc tgcatctgat
     1321 caaaactata gatatgcgaa tacacaagaa tttataaata aaataaaaag taagtatgac
     1381 attgatttta ttactggaca ttcactaggt ggaagagatg cggtagttct aggaatgagt
     1441 aatggtattc cgaacattgt ggtttataat ccagctccta tttctataac tagtttgaat
     1501 cctaattccc cagatggaaa acgtttatta gaattatata aaaattataa aggtaatatt
     1561 actaggtttg ttgcagaaaa tgatgcattg acagaaaatc tgaagaaata taagcattat
     1621 gtttttttcg gtaatgataa agtctttaaa aatggtaaag gtcatgaaat ggaaggcttt
     1681 ctgaccgaag aagaacaaaa agctataaaa aaagaactta aaaaactaca aggttatgca
     1741 gaagaaaata ataagtcatt tgtaaagaat tcaaataatg ctatctctaa attagctagt
     1801 atagaattac ttagagcaaa tatgatgact acaaatggag gaggattatc ttcttcgcag
     1861 caaaaagttt tagaaagttt aacagcttta acaattgcgc agtcattcag tcaactgata
     1921 gacgatgaaa ttaatcaaat caaaaaaatg tataatgaaa agaaaaagaa atttggaaaa
     1981 aattgggaag acgctcaaaa agctggaaaa gctgtaggtg aagatttgag tgtaaatgga
     2041 gttctcaatg ctttagatga aggtcaagtg aatgaaagta gtatggtaag agaacctgaa
     2101 caaatgatat ctgcaaaaga aagacaactt tcaacgatag ggtcttctgt atcaaattat
     2161 attatgagag ttagactcag tattaatgaa atcgttgata aagatcaagt gcttgcatca
     2221 caaataggtg gattactatg atgtttaatc aaattaataa taaaaatgaa ttagaagaat
     2281 catatgaatc tgagaaaaaa cgtatagaga atgaactgca aaatttaaat gaacttaggc
     2341 atagaactcg aaaagaaaat gagcgtagtt atgatgtttt tcaatatttg aagcacgaaa
     2401 tgaattatag tgaagatgcc caaaggaaaa tgacgagaaa tatagaagcg tatgagcaag
     2461 aaatcaatga gataattaga aagcaagaat ggaaattaga agaatataaa gaagacttaa
     2521 aaaagtctta tgaaaagcag ttagataagc taagtgactg ataatttgga ggagattaaa
     2581 tgactctaat agaaccagat atgaccttaa gaatgccaga tataagtact acagtagaaa
     2641 cacttaatct catatctaaa atggaagcgc aaaaagaaaa tattcgcaca gttattgcac
     2701 ctgaacataa gcataaatac aaagatattg aaaacggatt aaaaggtgaa gaaaaagtat
     2761 taattgaaca aatggcgcaa cattgcgaag cttttaaagc taattttaaa ggtgcagctc
     2821 aaggagattg ggttaaaagt gccatgtctg agatagacag cattaaggat gacctgaaaa
     2881 aaattaatag ctaaactaca tatcatacta ctaaataaag cgaccaatgt tcagtatatt
     2941 cacaactgac actaggccgc ttctttttaa tttatattat tcccatgtaa agtaattcga
     3001 accgaatcga aatatcacac tctcaattgt aaaaaaacaa cttaaattac tgtcccgcat
     3061 taaacatgac cacattcgta acagcaacaa taaaaatcct caagtactaa aagctatgat
     3121 cactttatca aaatcaacgc aagttaacct ctcccgaact acccgttact catcaccttc
     3181 accttacaaa ccataaagcg ctgcagttaa gtattgaatg gcatagccaa ttgaaatgaa
     3241 aatagcaatc gttataatag cactgccaat ggtatataaa gtatgtttcg tagataatcc
     3301 ttcagtcttt gaaccaaaaa cactgagtag aataaatgca ataccagtaa catataaaac
     3361 taaaatgaca aataacatgt ttatacacct cgattctctt tcgtgagata tatctttgtc
     3421 tccattataa agtaaagcgc tcaataattt aattaagtat actatttttt tattatcgaa
     3481 gattgcgaaa gaggaaacgc tcctatataa tgtaaaacgg aattattcct atttacaaat
     3541 aaattgaagt gagggtatat atatggctaa aattccagtt acggtattaa gtggttattt
     3601 aggctcgggg aagacaacgt tgttaaatca tattttacaa aatcgagaag gtcgacgtat
     3661 cgcggtaatt gtaaatgata tgagtgaagt aaatatcgat aaagatcttg tcgcagatgg
     3721 tgggggacta tcgcgtacag atgaaaaatt agtcgaactt tctaatggtt gtatctgttg
     3781 tacacttaga gacgatttat taaaagaagt tgagcgttta gtgaaaaaag gtggcatcga
     3841 tcaaattgtt attgagtcaa cagggatttc agagccagta cctgttgcac aaactttctc
     3901 atatattgat gatgaacttg gcattgatct tacagcgatt tgccgtttag atacaatggt
     3961 tacagttgtg gatgctaacc gcttcgtaca tgacatcaac tcagaagatt tattgatgga
     4021 tcgtgatcaa agcgttgacg aaacagatga gcgttcgatt gctgatttat taattgacca
     4081 agttgaattt tgtgatgtat tgattattaa taaaattgat ttaattagtg aagaagaact
     4141 agcgaagtta gaaaaaatgt taagcgcatt gcaaccgact gctaaaatta ttaagacaac
     4201 aaattctgaa gtagatttaa aagaagtctt gaatacgcag cgttttgatt ttgaaaaagc
     4261 gagcgagtca gcaggatgga tcaaagaact tgagtctggt gggcatgcat cgcatacacc
     4321 tgaaacagaa gaatatggta tatcatcgtt tgtatataaa cgtcgtctac ctttccatgc
     4381 taaaaggttc aatgattggt tagaaagcat gccaaataat gtcgttcgat caaaaggtat
     4441 cgtatggcta gcacaataca atcacgtagc atgtttatta tctcaagcag ggtcatcttg
     4501 caatattcat ccagttacat attgggtggc tagtatgtct gaagcgcaac aaacacaaat
     4561 attagcagaa cgtcaagatg tcgcagctga atgggatcca gaatatggcg atcgtcatac
     4621 acaatttgtc attattggta cagaattaga tgaagaaaaa ttaacaaaag aactcgacgc
     4681 atgcttagtc aatgcgcaag aaattgatgc agattggcaa caatttgaag atccatatca
     4741 atggcaaatt agaccagcac gataagttga atgaagtata atcatttttg aattgtggct
     4801 taattgtttg caatttatga caattaataa aaagttaagg tgttcttagg atctttttgt
     4861 tttcaatcta tctaaagtgt gatagttttg ataaagcaga aatttgcatt ttatccatag
     4921 ggctaggaca tgtatgtgtc ttagtccttt ttatatttac gttgatgaaa aatggcaaaa
     4981 ccatcgtacc aactgttaat tgaaggtaca atcattgttt aacagtcata aaaatgagtg
     5041 aagtaaatgc agctatcaat gtactattaa cacttacggt cacacgagtg tttcagtcta
     5101 aacgtgcaac gcaattccgc acgtggccaa tatatcttgc gctagatcat gaaaatcatt
     5161 aagatttcgc atgccaaatc atgaatgtct cagaactcga gtatcaaaca taaaacacgc
     5221 cacaaaaata taacgcgtca gactacaaag atcacgaata ttccgtatgt cccactaaga
     5281 acgtcctata caaaaaacac caagaccatt tatttgcaac aaactaacaa gactcacctc
     5341 acatcaataa atcaacacaa agcaaagcca ccatccctat tggtatagtg gcctgagaat
     5401 tttaagtatt caattcgctt aaattatttt gcgaaaatgt cgataattgc tttgatgatt
     5461 ttaatgatag tacctacaat agccatcgtt ttgtcctcct gtatgttgat ttaacatagt
     5521 gatgagttgt tgatcgttaa tgtttgagat tagttgttac ctaaaaattt accaagtaaa
     5581 tctttaaaga atttgaataa ttttgctacg aattccatgt gaatggcccc cttcaaataa
     5641 gatgttcata tctatgagat ttttataact tacttaccag tgaatttctc aattaatcct
     5701 ttaatgaatt taatgattcc tgcaatgata cccatgtgaa agacctcctt tgtttgttat
     5761 gaaatcttat ttaccagtga attgttcgat taagcttttg ataactttaa tgatgccagc
     5821 gatgataccc attaagatta cctcctttgc ttatgagtta acttcattgt acatagttat
     5881 cttgtgcgta attgattttt ttcctaactt gaattgttat gatcataact gcgaattttt
     5941 tattttaaaa ggacgggaaa tactgcctaa ctgtacaggt gcatttacaa aatctttaca
     6001 atcattggtt taatagaggt taagctcatg aatttgacat gaagcagaaa tttaattata
     6061 ttttatgtta taagtttaaa acgaataaca cgttaggtct cgtctaggca aagcatattt
     6121 atgaagatga attgaacaga cagaagtaac attttatata catatgtgca tgggagcacg
     6181 cttaaagaga acactgcaac aaggtatatc aacgtaccgt gatgcagtgt ctagttaaac
     6241 tgaaacctgc aatagtaaat tttctgccat tatgtacaga atctactatt gtaggttttt
     6301 atttttatac ataagcaatt atgcgagaga ttagaaataa ggaggttatg cagtgttaag
     6361 ttttcaattg ctattttcac tgtttgttat tgcgcttatc attgcattga taagtggctt
     6421 gttgttttta gcaccagtta tgccaatgag atatattaaa ttacatttat acatactagt
     6481 catgccagta ttatttgcag tcattggctt tttcggtatt catggtcaac atgtcttagg
     6541 tccatttaaa atagatcgtt tatcttggtt attagctggc tttgtaatgg cgcttggctt
     6601 tattattcaa aagttttcaa tgcgatattt actaggtgat catcattata gacattactt
     6661 tccattgttc actgcgatta cgtcgtttgc atctttagca tggatgtctg aagacttaag
     6721 actgatggca ctctgctggg gtattacatt attatgttta acattgctga tgaacgttaa
     6781 tcgtttttgg aaagtgccac gtgagtctgc gaaattatca agcatgacat ttttatgtgg
     6841 ttggcttgca ttcgttggag caattgtaac tatttatatt gcgactggcg agtggcgggt
     6901 accacaacat atagttcatc cgacatggtc attgttgacg aatgtactac ttgtattagc
     6961 tgtcatgata ccggcagcac aatttccttt tcatcgatgg ttgattgaat ctgtaacggc
     7021 accaacgcca gtatcggcaa ttatgcatgc aggaattgtg aatgcaggtg gtgttattct
     7081 aactcgtttt gcgccgatat ttgataatgg atttgcgtta tcattattac ttatcctttc
     7141 tagtatttct gtattgttag gatcgggtat tagcttagtt caagttgact ataaacgcca
     7201 attagtgggc tctacgatga gtcaaatggg ctttatgtta gttcaatgtg cattgggtgt
     7261 atattcagca gcgattattc atttaatatt gcacggtatt tttaaagcaa cattattttt
     7321 acaatcaggt tctatcgtga agcgattcaa tattccaaaa caagcatctg ctaaagacgc
     7381 ttatggctgg attgtcatgg gacgtgtatt agctattatc gtggcatttc tattttggat
     7441 gagtagtgac agaagtgcat atgaagtgtt aagtgcactc attctaggat ggtcattact
     7501 tgtatcttgg aatcaaatgg tagcctttag taaaggacgc atggcacgtc ttgttggtat
     7561 gattttgatt gcaatcgtga catttatcta cgtcatcaca cataattatt tttacgctgt
     7621 attacaaaat ataacaacac atgcgacaac gccgcctaca gtgagtgtca tcattagtgt
     7681 tgttatttta atctttggca gtttattaag tatttgggtg gcgcggcatc gatactctaa
     7741 ggcttttgcg gtattgtacg tgtggttagt taatctaggt gaagcacgct cgaaagcgat
     7801 agaaagtcat ccgaattatt tgaagaagta tttataggag gtgaaaggta tgacaacaca
     7861 gttaaatatc aattcagtca ttgaaaatgc gaaacgtgtt attacaccat tatcaccaat
     7921 ttcgattttt gcagcacgaa atccatggga aggattagaa gcggatacgt ttgaagatgt
     7981 cgcaaaatgg ttacgtgatg ttcgagatgt ggatattttc ccaaataaag cattaataga
     8041 aagcgctgtg gcacgtggtg aattagatga aagtgtcttt aatcaacttg ttactgatat
     8101 gttacttgaa catcactaca atatcccgca acactacatc aatctttata ttgataacat
     8161 taaaacatta aaagacgtac ctgcatcata tatgaatcat tcaaatgttg atgttgttgc
     8221 tgatctacta ttagaaaaat caaaacgtga tatggctgaa tcatatcatc actatgatgt
     8281 acgtccgatg agtgatgcaa taatagatga acaaggtgag ccacttagcg aacaagtgaa
     8341 tcgtcaaatg attaaatgga cgaaacttta tatcgatcaa tttctatcga gttggacaat
     8401 gccgaagcgt gagcaaagtt tttaccatgc atggttgcat ttagcgcaac atgaccatag
     8461 ttttactaaa gcacagcgcc aagtgattaa aggcttaccc aatgatcctg aaatgacgat
     8521 agagtcagta ttaactcatt tttcaataga tcaggaagac taccaagctt atgttgaagg
     8581 acatcttttg gcgttaccgg gttgggcagg tatgttgtat taccgttcac aacagcatta
     8641 ctttgaacaa catttgttaa cggattattt ggcaattcgg ttagttgtcg aacaattgct
     8701 agttggtgat gagtttaagt cagtcgctaa agattgtgaa agtagatcgg aaaattggtt
     8761 taagcaaact gttgcatcat ggtgttacta cagtgatatg cctagcgatg tattactaca
     8821 acatgacgtc aatgaaattc aaacgtttat tcattttgca gcaactatga ataaaaatgt
     8881 atttaaaaat ttatggctaa ttgcctggga aatgacatac gaatctcagt taaaacaaaa
     8941 aattaaagca ggtcatgaaa gtgtggcggg cgcattagat gtaaaccaag taaatgtctc
     9001 agaaaatgat aacgctaatc agccacattc agtattgtta aatgacacac aagcagttga
     9061 tgaaaataat agcgagctaa atcagatggg cacatcaacg aaagcgcaaa ttgcattttg
     9121 tatagatgtt cgttcagaac catttcgtag acatatcgaa gcagcagggc cctttgaaac
     9181 gattggtatt gcaggcttct ttggattacc tattcaaaaa gatgccgtag acgaacaatt
     9241 caaacatgat tcattacctg tcatggtacc gccggcatat cgcattaaag aatttgcaga
     9301 ccgctacgat atgaatgttt atcgacaaca gcaacagaca atgtcatcga tgttttacac
     9361 atttaaattg atgaaaaata atgtcatgcc tagtctgtta ttgcctgaat taagtgggcc
     9421 atttttaagc ttaagtacca ttgtcaattc gattatgcct agaaaaagtc gcgcgtcttt
     9481 acaaaaaata aaacaaaagt ggttgaaaaa gcctgaaaca aagttgacga ttgatcgtga
     9541 gtttgaccga acatcagact tacctgttgg atttactgag caagagcaaa ttgatttcgc
     9601 gttacaagcg ttgaaattga tggatttaac cgaagcattt gcgccgttcg ttgtgttagc
     9661 aggtcatgct agtcattctc acaataatcc acatcatgca tcacttgaat gtggggcttg
     9721 tggtggcgca tcaagcggtt ttaatgctaa gttattagcg atgatatgta atcgtccaaa
     9781 tgtcagacaa ggattaaaac aatcaggtgt gtatattcca gagacaactg tttttgcggt
     9841 agcagaacat catacgtcta ctgatacgtt ggcatgggta tatgtgccag acacattatc
     9901 ttctattgct ttagatgcat atgaatcatt gaatgacgcg atgccgatga tttctgaaca
     9961 cgcgaatcgc gaacgtttgg acaaactgcc aacgattggt cgtgtgaatc atccagtgga
    10021 agaagcgcag cggtttgcga gtgattggag tgaggtacgt ccagaatggg gattggctaa
    10081 aaatgcatca tttataattg gacgacgcca attaacaaaa ggcattgatt tagaagggcg
    10141 gacattttta cacaattatg attggcgtaa agataaagat ggcacattat taaataccat
    10201 catttctggt ccggcacttg tggcacaatg gattaattta caatattatg cgtcgacagt
    10261 tgcgccgcat ttttacggaa gtgggaataa agcgacacaa accgtcacgt caggtgttgg
    10321 tgtcatgcaa ggtaatgcga gtgatctgat gtatggctta tcatggcaat ctgttatggc
    10381 tgctgatcgg acgatgtatc attcgccaat tcgtttactt gtcgttattc aggcacccga
    10441 ctatgttgta gcaagactac tcgcgaataa tgagcatttc gctaggaagg tgtctaatca
    10501 ttggctgcgt ttaatgagcg ttaatgagga agggcgtttt aaaagttgga tttaacgatg
    10561 atatgttgcg atgctaagta actaatatat catcatatct acaatgttct agcttcttaa
    10621 aatatactaa aaatgataaa cttaatgtaa tagttgatgt cgtgataacg atacaaagat
    10681 gaagtaatca attttaatat tattggagtt agtgatatga aaagaacgaa aggtgaaatc
    10741 gaagctgaaa tcagtaaagc cattacgcaa tgggaaaaag atttccttgg cagaggttcc
    10801 ttgtcagtta aatcagacat tttaagagat atggtgatta ttagtttaca aggtatctta
    10861 acgccagctg aatatcgtgt atgtagtacg aatgaaggat tattaaatat taaacgaaca
    10921 cgttctgaat tagttgaatc cggtgagcaa gatttgaatg atatcatttt taaaattaca
    10981 ggtatcaaag tgatgagctt ccatagtgat ttaagtacag ttacaggtga acgtattatc
    11041 gtattcaaac ttgaggataa tttggaaaag catatttaag aaggttaggt atgcttctgc
    11101 tggcttatta aagctataga agtatgcttg gttaaacagc gcaacgccat atatttgggt
    11161 gtgctgtttt tctattacat gatgtgttta ttacgtggat gaatgcgtgg gtctgaatat
    11221 agcccgtgaa aggcgtatgt cataaaattt gcgaatggtg acaagtcgac gcaagcatat
    11281 ttgttgcgag acatgcccac tatcctttta cataaagagt atatgtgtga cgtaggcata
    11341 taatcgataa agtattccta aaaaattagg tataatgacc attgttgcaa taagtttttc
    11401 ggccttgaaa ccgataacgc atagtataat aggaataaaa tataatacaa atatccagta
    11461 aatcaaattt gaaaatgatg gatgaatagg ggtaaagaat ctaactaatg taatgattaa
    11521 cgctatatag acaaaaataa ctctattgat acgccttacc ccctctgtat aataaatata
    11581 aatcgtacta aattgaaaat agcaaaatat tgttatttca attatacaat gttttatttg
    11641 caatatacat aacaatatga actttataat tttgatattg tcggtaaagt gaaatttgtg
    11701 gcacattgat attatggata acatttgtaa aatgaaaatg atgatacgag gagattgtaa
    11761 gatgatagat aaaaaattaa catcaccgaa aatgacagtg cctttatttt taatcgcgct
    11821 gattgtattt ataggtatgt tttacagtgt agtgacaaat caagaatggc ttaaaaatat
    11881 agatatggga tcattaacat ggtttacaga ttatttcggt gagccacaac gtcagtatgt
    11941 taacaatttg tttaattact atatgacgtt tagtgcggaa attggagatg tcaaaggtgt
    12001 cgtgttgatt tctattatcg tcacaatcat actgtttatt aaacagaggc atttagcggt
    12061 ttggtttgtg acatatttgg tttcaggtgt catcatgaac aaattaatta aagatactgt
    12121 attacgtcca agaccatata atcatttagc cgttgataca ggcttttcat ttccgagtgg
    12181 acattccaac gccagcacat tattatattt cgccttaatg atcattatta tttcacttgc
    12241 tgctaagaca ataacaaaag tgttgagtgc gttggttatg ggaatattat ggcttagcat
    12301 attattttgt cgcctttatt ttcatgcaca ttacttttca gatgtcattg gcggcacgtc
    12361 actagcaatc atttgggtag cgttattctt aatggtatac ccatacttta ttaatcatcg
    12421 acgacaacgc gtttagcaaa catctttaga catagtggag gtatatagaa aaatgagaat
    12481 taaaacaccg agtccatcgt atttaaaagg cacaaatgga catgcgatat tattattaca
    12541 ttcatttaca ggtacaaatc gggatgtgaa gcatcttgca gctgagttaa atgaccaagg
    12601 atttagttgt tatgcaccga attatccagg tcatggttta ttgttgaaag atttcatgac
    12661 atataatgta gatgattggt gggaagaagt tgagaaagct taccaatttt tagtcaatga
    12721 aggttatgaa tctatcagtg caacgggtgt gtctttaggt ggattaatga cattaaaatt
    12781 ggcgcaacac tatcctttga aacgtatcgc tgtcatgtca gcaccaaagg aaaagagtga
    12841 cgatggctta atagaacatt tagtttatta tagtcaacgc atgtcgaata ttttaaattt
    12901 agatcagcaa gcatcgagtg cgcaattagc agcaattgat gattatgaag gtgaaattac
    12961 gaagtttcaa cattttattg atgatatcat gacaaattta aatgttatta aaatgccagc
    13021 taatatatta tttggtggta aagatgcgcc atcctatgaa acaagtgcac attttattta
    13081 tgaacattta ggatcagtag acaaagaatt aaatggtctg aaggattcgc atcatttaat
    13141 gacgcatgga gaaggcagag atattttaga agaaaatgtt attcgctttt tcaatgcttt
    13201 aacataattg aagattttaa aaattaacac ttgagtgacg atgttattat atttgtcatt
    13261 tgagtgtttt ttgtggcgtg atagtatagg gaacttagct aaagttaatt tgtagtgtga
    13321 tgtcgtggaa atattatgga gttataccag aagttgattt gtattgtaat gtcgtggaaa
    13381 ttttatgggc attgcacctg aagttgttat gtggcgagat gttggggata tagatggtgt
    13441 tgtatgtggc gagatattgg cgcacgtata cagcgttgaa ccagaaaatt taatgtagcg
    13501 taatgacaac acattacaag agttgattat attatgataa atccaggtgc taccgtatta
    13561 atgaaacaat tttgatgcag gtgtattgca tgttatttac atacatgtta gagtgttact
    13621 taaacacgta gacgctttaa cgctttaaag gagatgtgaa tgatgaaaag acaacaatca
    13681 caatggaagt catcaactgg atttatttta gctagtgcgg gttctgcaat cggtcttggt
    13741 gccatgtgga aattcccata tatggcaggg atttatggcg gcggtgcctt tctagctatg
    13801 ttcttaatat tcaccatttt tgttgggttg ccattactca ttatggaatt cactgttggg
    13861 aaaatgggac ggacatatac aacacaaata tatagtaaat taactggtaa aaaatggctc
    13921 aatatcattg gctggaacgg taatttggca gtgtttattt tatttggctt ctatagtgtt
    13981 atcggtggtt ggattgtcat ttacatcgga caagtattat ggcaattagt tatatttcaa
    14041 cgcatcaatc atctccaaga aatgaatttt gaagcggtaa tatcaaatcc ttggttaacc
    14101 gttctagggc aaggtatatt catattcgct acgatgatta ttgtcatgtt aggtgttgaa
    14161 aaaggattag aaaaggcatc gaaagttatg atgccattgc tgtttgtctt tttaatcgtc
    14221 attgtgatta agtctttaac attagatggc gccttagaag gtgtgaaatt tattttacaa
    14281 ccaagagtat cagagattac tgctgatggc atcttgtttg cgctaggtca atcattcttt
    14341 acgttatcat taggaactac aggtatgatt acttatgcga gttatgcctc taaagacatg
    14401 acgattaagt catcagctat ttctatcgtt gttatgaata tctttgtatc tgtattggca
    14461 ggtctagcta tatttccggc tttacatagt tttggctatg aaccacaaga agggcctgga
    14521 ttattattta aagtactgcc aatggtcttt agtcaaatgc atctaggcac attattctat
    14581 ttgggattct tagtgctgtt cttatttgcg gctttaacgt catctatttc tttattagaa
    14641 ttaaatgttt ctaacttcac gaagaatgac aatacaaaac gtaaaaaagt cgcagtgatc
    14701 ggtagtattt tagtatttat cattagtatt ccagcaacct tatcttttgg tatcttaaaa
    14761 gatgtaagat tcggtgcggg aacgattttt gataatatgg atttcatcgt ttcgaatgta
    14821 ttgatgccat taggcgcatt aggtactacg cttgtcgtag gacaattatt agataaaaaa
    14881 ttattacaac aatattttgg taaagatcga tttagattat tcagtggttg gtattactta
    14941 attaagtatg cgatgcctgt cgttattatt ttagtcttta tcgtgcaatt atttagttaa
    15001 tatatagaag catctgacac acaagtattt gtgttgctca ggtgtttttt tgtttggaat
    15061 tgaaaattta gtactggttc tgaattaatc gaaacttaga taattgcaaa ataacaaaat
    15121 gtcgtttata attcttatct gaaaagtaag aattaaaatt gagacatgaa gaaaggtttc
    15181 attcatttga tcggcatgtc agatgagggt gcatctatga ttacttatga tttaattggc
    15241 aatacaccat tagtactgtt agaacattat agtgatgata aagttaaaat ttatgccaag
    15301 cttgaacaat ggaatcctgg aggcagtgtt aaagacagac tcgggaaata tttagtagag
    15361 aaggcaattc aagaagggcg tgtgcgtgca ggtcaaacta ttgttgaagc gactgctggt
    15421 aatacaggca tagggttagc tattgcagcg aatagacatc atttgaaatg taagatcttt
    15481 gcgccgtatg gtttttcaga agaaaagatt aatattatga tagcgcttgg tgcagaagtt
    15541 tcaaggacga gtcagtctga aggtatgcat ggggcacaat tagctgcacg ttcctatgct
    15601 gaaaaatatg gtgccgttta tatgaatcaa tttgaatccg aacataatcc ggatacatat
    15661 tttcatacat tgggacccga attgacttca gcattacagc aaattgatta ttttgtggct
    15721 ggtattggct ctggcggtac atttacaggt accgcacgtt atttaaagca acatcacgtg
    15781 caatgttatg ccgttgagcc agaagggtcc gtgttaaatg gagggccagc tcatgcacat
    15841 gacactgaag gtatcggttc tgagaaatgg ccgatatttt tagagagacg tcttgtagat
    15901 gggatattta cgattaaaga tcaagatgcc tttcgaaatg tcaaaagttt ggctataaat
    15961 gaagggttgt tagtaggcag ttcttcaggt gcagcattac aaggtgcatt gaatttaaaa
    16021 gcgcaattat ctgaaggtac gattgttgtc gtatttccag atggtagtga tcgatatatg
    16081 tctaagcaaa tatttaatta tgaggagaat aataatgaac aagaaaacta aattaattca
    16141 tggtgggcac acaacagacg attatacagg tgccgttaca acaccaattt atcaaacaag
    16201 tacatattta caagatgata ttggtgattt acgtcaagga tatgaatatt ctcgtactgc
    16261 gaatccaaca agaagttctg tagaaagcgt tattgcgaca ttagaaaatg gcaaacatgg
    16321 ctttgcattt agttcaggtg ttgcagcaat cagtgcagtt gttatgctgt tggacaaagg
    16381 agatcatatt attttaaatt cagatgtata cggcggtact tatcgcgcat tgacaaaagt
    16441 atttacacga tttggcattg aagtggattt tgtagataca acgcatacag attcaattgt
    16501 acaagcgata cgcccaacaa caaagatgtt gtttattgaa acaccttcta atccattatt
    16561 acgtgttact gacattaaaa agtctgctga aattgcgaaa gaacacggtt tgatttcagt
    16621 tgttgataac acatttatga caccttatta tcagaatcca ttagatttag gtatcgatat
    16681 tgtcttacat tctgcaacga aatatttagg tggacatagt gatgtcgttg ctggtttagt
    16741 tgcaacatcg gatgacaagc ttgcagaacg tttagcattt atttcaaatt caacaggtgg
    16801 cattttagga cctcaagata gctatttact tgtgaggggt attaaaacat taggtttacg
    16861 tatggaacaa attaatcgca gcgttattga aattattaaa atgttacaag cacatccagc
    16921 tgtgcaacaa gtgttccatc caagtattga aagtcattta aatcatgatg tccatatggc
    16981 tcaagcggat ggccatacag gtgtgattgc atttgaagtg aaaaatacag aaagtgccaa
    17041 acaattgatt aaagcaacat cgtattacac attagctgaa agtttaggtg cagtggaaag
    17101 tttaatttca gtacctgcat tgatgacaca tgcatccatt ccagcagata ttcgagctaa
    17161 agaaggtatt acagacggac ttgtaagaat ttctgtaggt attgaagata ctgaagattt
    17221 agtcgatgat ttaaaacaag cactagatac tttataaata atagcagcac tggcatatat
    17281 tttgagttca acgtttgttg ttcttgaata tgctagtgct tttttgtgcg atgttaatat
    17341 ttagtaattg cgttgtggag gattggtgga catttttatg gatttaattt gataaatgtc
    17401 atagtagtct cacaattcgt cattgtcaca tgaattactt attttttaat tttttagaaa
    17461 attcggcaaa ttgtattgac gatatatgca attttacata taatgctctt attcctagtg
    17521 gattaataag aatttgtagg aggggcgatg atgattgagt ttcgacaggt aagtaagacc
    17581 tttaataaaa agaagcaaaa aatagatgct ttgaaggacg tatcatttac ggtcaatcgc
    17641 aatgatattt ttggtgtgat tggatatagt ggtgcaggaa aaagtacgtt ggtaagactc
    17701 gtgaatcatc ttgaagctgc ctcgaatgga caagtgattg tagatggaca tgatattacg
    17761 aattatagcg ataaaatgat gagggatatt aagaaagata tcggtatgat atttcagcat
    17821 ttcaatttat taaattcagc taccgtattt aaaaatgtag caatgccact cattttaagt
    17881 aagaaaagca aaacagaaat taagcaacga gtaacggaaa tgcttgaatt tgtaggattg
    17941 agtgataaaa aagaccaatt tcctgatgaa ttatctggtg ggcagaagca aagggtggct
    18001 attgcaagag cgcttgttac taatccgaaa atactcctat gcgatgaagc aacaagcgca
    18061 ttggatccag caacgactgc ttcgatattg acgttattaa agaatgtcaa tcaaaccttt
    18121 ggcattacaa ttatgatgat tacacatgaa atgcgcgtta ttaaagacat ttgtaatcgt
    18181 gttgctgtaa tggaaaaggg gaaagtggtt gaaacaggaa ctgttaaaga ggtgtttagt
    18241 catcctaaaa cgacgattgc tcaaaatttt gtgtctacag ttatacagac tgagccaagt
    18301 acatcattga ttcgtcgatt gaatgacgaa caagttggcg attttaaaga ttataaaatc
    18361 ttcgtcgagg aaactcaggt gacacaaccg attataaatg acttgattca aatttgtggc
    18421 agagaggtta aaattttatt ttcatctatg tcagaaatac aaggtaacac cgtatgttat
    18481 atgtggcttc gatttaatat ggatcaacaa tttgaagaca cggcaataaa tcaatatttc
    18541 aaagagaaaa atattcaatt tgaggaggtg cattaacata tgtttggttc tgatttagac
    18601 agtgcacagt tattacaagc attgtacgaa acgctatata tggtatctat tgctttattt
    18661 ttaggagcag tgattggtat tccattaggt gtcttattgg taattactcg aaaacaaggc
    18721 atatggccca atatagtgat acatcaagtt ttaaatcctt taatcaatat tttaaggtca
    18781 ctaccattta ttattttgtt aattgcgatt gtgccattca caaaattagt agtaggtact
    18841 tcaattggta cgactgctgc catcgtgcct ttaacagtat atgtggcacc ttacattgca
    18901 agacttgttg aaaactcatt attggaagta gacgagggga ttattgaagc ggcgaaagcg
    18961 atgggcgctt caccactaca aatcattaga tattttttaa ttcctgaagc tttaggttcg
    19021 ttagtattag caattaccac tgcgattatt ggacttattg gaagtacggc gatggcagga
    19081 gctgttggcg gtggtggtat aggagactta gctttagtgt atggttatca aagatttgat
    19141 acgacggtca ttattattac cgttattgta ttagtcatta ttgtccaagt gattcaaacg
    19201 ctagggaatg ttctagctag attcatacgt agacattaat gatatatagt gaagattttg
    19261 aaaggaattg atagaatgaa aagattgatt gggttagtta tcgtagcact tgtattatta
    19321 gcagcgtgtg gtggtaacaa tgataaaaaa gtaacaattg gtgtcgcatc aaatgacact
    19381 aaggcttggg agaaggttaa agaattagct aaaaaagatg atattgatgt ggagattaag
    19441 cacttctcag attacaattt accgaataaa gcattaaatg atggtgatat tgatatgaat
    19501 gcattccaac attttgcatt tttagatcaa tataaaaagg cgcataaagg aacaaagatt
    19561 tcagcattaa gtacaacagt tttagcaccg ttgggcattt actcagataa aattaaagat
    19621 gtcaaaaagg ttaaagatgg tgctaaagtt gtcattccaa atgatgtgtc aaaccaagca
    19681 cgtgcactta aactattaga agcagctggt ttaataaaac tgaaaaaaga tttcggatta
    19741 gcaggcacgg tgaaagatat aacgtcaaat ccaaaacatt taaaaattac tgcagtagat
    19801 gcacaacaaa ctgcacgtgc tttatctgat gtcgatattg cagttattaa taacggtgta
    19861 gcaactaaag cgggtaaaga tcctaaaaat gatccgatat ttttagaaaa atcaaattca
    19921 gatgcagtaa agccatatat taatattgtt gcagttaatg acaaagactt ggataacaaa
    19981 acatatgcta aaatcgtaga attgtatcat tcaaaagaag ctcaaaaagc gttgcaggaa
    20041 gatgtcaaag atggagagaa acctgttaat ttatctaaag atgagattaa ggcaatagaa
    20101 acgtcattag caaaataaat tatattgcgt cctacaagca aagttcatgc ttatgtttgt
    20161 agggcgttat tgttggagaa taaaattatt tccaatagag aaagggattg taatcatttt
    20221 atagtgaaat attatgaaat tgtaataatt tagatattgt aaaatctaat aagttgtaat
    20281 aattttaagg ggtaattata aaatttgatg atacagtata tgattttttt gtaatcataa
    20341 tgtcatcaaa catcaaccta ttatacataa taaaatcgta taatgatgta gtattcataa
    20401 attcggataa aagaatgtta ggaaagttaa gcaagaggag gattttaaag tgcaaaaaaa
    20461 agtaattgca gctattattg ggacaagcgc gattagcgct gttgcggcaa ctcaagcaaa
    20521 tgcggctaca actcacacag taaaaccggg tgaatcagtg tgggcaattt caaataagta
    20581 tgggatttcg attgctaaat taaagtcatt aaacaattta acatctaatc taattttccc
    20641 aaaccaagta ctaaaagtat ctggctcaag taattctacg agtaatagta gccgtccatc
    20701 aacgaactca ggtggcggat catactacac agtacaagca ggcgactcat tatcattaat
    20761 cgcatcaaaa tatggtacaa cttaccaaaa cattatgcga cttaatggtt taaataattt
    20821 ctttatttat ccaggtcaaa aattaaaagt atcaggtact gctagctcaa gtaacgctgc
    20881 gagcaatagt agccgtccat caacgaactc aggtggcgga tcatactata cagtacaagc
    20941 aggtgactca ttgtcattaa ttgcatcaaa atatggtaca acttatcaaa aaattatgag
    21001 cttaaatggc ttaaataatt tctttatata tccgggtcaa aaattgaaag taactggtaa
    21061 tgcatctacg aactcaggat ctgcaacaac gacaaataga ggttacaata caccagtatt
    21121 cagtcaccaa aacttatata catggggtca atgtacatat catgtattta atcgtcgtgc
    21181 tgaaattggt aaaggtatta gtacttattg gtggaatgct aataactggg ataacgcagc
    21241 ggcagcagat ggttacacta tcgacaatag acctactgta ggttctatcg ctcaaacaga
    21301 tgtaggttac tatggtcatg ttatgtttgt agaacgtgta aataacgatg gtagtatttt
    21361 agtttcagaa atgaactatt cagctgcacc aggtatttta acttacagaa cggtaccagc
    21421 ttaccaagta aataattata gatatattca ctaaagtctt acgtatataa atatataatg
    21481 aattcctatt acatttcaca agctgaaaag ttggattgaa tcctaaattt tatggcgggg
    21541 ctatcaaagt cgtgattgtt gtaataggaa tttttgtata tgaaaaagga ttggtcgaat
    21601 gaacttcatg ttctatgttc gaccaatcct tttctgaatt agtattcatg ttttactttg
    21661 cgaatgcttg taaataatct agcaccgttt gttattaaag taacaactgc cactgctttt
    21721 ttgaacttac gtggtgactt aattgaaatg taaaagtcta acacggttcc tacagtgctt
    21781 aaacctaatg aaagatatac taatttttta tttttagcat gatatttata gccattgtag
    21841 ccgtcgacta tgaaacctgc gacatttagt aaacttgata aacgttgtga tttggaacgt
    21901 tttgccataa taataattcc ccctagtatc ttctgtttat atgaaaagtt gggtgcgctg
    21961 aattgctaac gttttgcgct ataactactc atatatgata acataattgt acagtataat
    22021 ttgaaaaatt gatttcacaa agttggggtg tcaaagatga ttaaatgtgt ctgtttagtt
    22081 gaagaaacag ctgataaaat attgcttgtt caagtaagga atcgcgaaaa gtattatttc
    22141 ccgggtggta aaatagaaga aggggaatca caagtacacg cgctgttaag agaagtaaaa
    22201 gaagaattaa atttaacatt aacaatggat gaaattgaat atgtcgggac aattgtaggt
    22261 cctgcatatc cacaacagga tatgttaact gagttaaatg gatttcgcgc attaaccaaa
    22321 atcgattggg aaaacgtaac tatcaataat gaaattacgg atatacgctg gattgataaa
    22381 gataatgatg cgttgattgc gcctgctgtc aaagtttgga ttgaaactta tggtggtaaa
    22441 catgacaaat aatgacacca tcatgttacg acattatgtc ccacaagatt attcgatgtt
    22501 agaagctttt caattaagtg aaagtgattt gaagtttgtt aaaacgccag aggaaaatat
    22561 tacagctgca atgtctgata atgaaaggta tcccatcgtt gtaatggatg gcaggcaatg
    22621 tgtggccttt tttacattac atcgtggaaa aggggtcgca ccatttagcg ataaccaaga
    22681 tgcagtattt ttcaggtcat ttagtgttga tcaacgttat cgtaatagag gaataggtaa
    22741 agtggtaatg gaaaaattgg cgtcatttat cacttcaaca tttcaggata ttaatgagat
    22801 tgtgttaacg gttaatactg acaatccaca tgccatggca ctttatcgcc aacaaggata
    22861 tcaatatatg ggagatagta tgttcgtcgg aagacctgtt catattatgg cgttaactat
    22921 aaaataaatt aaatttaaaa gcatctttac tcatcgtcga ccacaacaat taatgatgaa
    22981 taaaggtgct ttttgttata gatcatcgga caatttacta tagtaaaaag cgacctagtg
    23041 aacaattgac atatatccac aggtcgctta acttaagtta tattgctagt tgcgattaat
    23101 tgatagactc atcatttttg cgctgtcgag atggtctttt tattaaaaat gccgtaatcc
    23161 aagccgtaat cggaatactg attgcaacgg caataccgcc taaaataata gaaataaatt
    23221 cttgggcaaa tattttcgag tttataatat gaccaaatga atatttaagt ttgaaaaacc
    23281 aaataaataa agcaagttgg ccaccaaaaa aggcaaggta aatcgtgttc gcagatgtcg
    23341 ctaaaatttc tctaccaaca cgcatgccag attggaataa ttcgtattgc gtaagcgttg
    23401 gattcacttg atgcaattca taaatgggtg aactaatggt aattgttaaa tctatcacag
    23461 ctgcaataac agcaagaata atagtgaaca ccataaattg aaccatatca atgccaatat
    23521 tcattgaata cacatatgtt tcatcttgtt gttcggttga aaagccttgt agatgaccga
    23581 agtagaccga taaataaatg agtgtaatca acaatattgt tgtaacgata gtgctgataa
    23641 atgcagcttg tgttttaaca ttgtaactat tgagtacgaa taaattacaa gcgccaataa
    23701 taatgcagaa aaagaatgtg acgacataaa tcggtacgcc aaaaataatc aatacaatac
    23761 taataattaa aatagcgaaa tttaaaaata gggttaaata agagatgaat ccctttttac
    23821 ctccgaaaat tatcatcaga aagaggagca ataacgccaa tataaataca gcattcattg
    23881 tttcgccctc cttaatgttt caaatatttc cataaacaat attgtgatag gaattgtaag
    23941 tacgatacct ataccacctg ttagtgcgcg cgcaatttct aacgaccaat tcatcgaaat
    24001 agtataagtc actgtattgg cattttttaa aaagattaaa aacataggta gtgcaccgga
    24061 taaatatgag aataataaga tgttagtcat tgttcccata atatcttggc cgatgtttcg
    24121 cccagcaagc gcccatctcc tcattgaaat gtgtggcgta cgctgtaaaa tttcatgcat
    24181 accactagca attgtaattg caacatccat aatagcgcca agtgaaccta ttaacactga
    24241 ggctaggaag atatctttcg gtggtaatga taaaaagttc atcgtttcat atttaatgcc
    24301 tttaccatct gccatatata tgattaattc tgttaaacct atactcaaaa aagttccgat
    24361 aattgtactg gctatggtaa tgagtgtacg catatgccag cctgtaacga gcaataaagt
    24421 gagtattgtt gaacagatca tggcaatggt catgagtaag aataaattaa tattgctatg
    24481 ttgaatatga atgtaaattg cgattaatat ggcaatagaa ttcaagatta acgataaaat
    24541 cgattgcagt ccgactttgc gaccaaccaa taatacagtt aataagaaca aaccagtgat
    24601 gataaccgtt aaggtatcac gcttcttttc tataatataa gcatcactcg gcttgttaga
    24661 aatatgtaat aatacttttt cgtgtgtgcg aaatgcctca gaatctgctt gcgatttgac
    24721 gtactgatga ttaatcgtcg tcgtttctcc agcaaattga ccatttaata ttttgacttt
    24781 taattgattt ttatatttaa tatcacgatt attttgtgca tcttttgtag gtgtcgaaga
    24841 aacatgtttg acatctataa tttgaccaat tggtttgttg taaaagttct cattattgaa
    24901 tgtaaataaa atagcaccaa tgaatgcgat gcagaacaaa cctaaaatta tattaaatgg
    24961 ctttgtaaat aaatttctat atttcaaaaa caaaacccca attctatgaa tgaattaata
    25021 tggtgattat acgcccttaa ttttttattt tcaaagatat tactgctaag tgtaaaacga
    25081 aaatcatcat tgatagcatc gaattactta atggaatgta gacgttttag tcattaattg
    25141 ctgaataagt gttaataata tgccaatatc actctttgta taaggctcct ttgtaatagc
    25201 acatatcgtt ctttttaatt cagtatgatc taattttata tctatccatg atttagattc
    25261 tggtaaatgt atattttgtg atgaaatgat gtaaccttct ttttgacgaa ggagatactg
    25321 cgcaagtggt tggctactga ttgtgtatac atctgattta gtaatcttgc gcaattgttt
    25381 ttttacagtt tcggcaaatg gtgccaagca ataaatatga ctatgctcaa actgaattaa
    25441 tggtgggtgt gtcgccatcg taattggatc gtctgaaggc gcatataaat gatagtgctc
    25501 ttcgaataaa ggtagcatat gtaattgttt gtgtttacgt atttctggtg taagttccgt
    25561 gaaaccaatg tctatattcc catttaatac gctatttata attgtgtcat gttctaataa
    25621 gctcggtatg acatgtgtat cattttgtaa atgaaacgtt tggataagtg gtagtaacat
    25681 gtgggatacg tcactctcat catagccaat gtagatactt ttatttttag ttaatccatg
    25741 gctttgaaat tgttcaatcg tgctatctaa atgttcaata atacgcagag cttcattaaa
    25801 taataatttc ccttcagatg tgagcgtaat attgcgtcct tgctttttaa ataaagacac
    25861 attaagttct tgttctaata atgtaatttg acggcttatc gctgattgag caatgtttag
    25921 ttcaagtgct gtttcggaga tatgttctct tttagcgacc tcgataaaat atcttaattg
    25981 tttaatttcc atagcgatat aggcacctcc aaaaatgagt gttttgtaac tattatagca
    26041 atattattga taaatgttct attttttaga tgaatatctt ctattttata tattgaaaag
    26101 ataaattttt tagattatag taattatcat taataactaa tatcagaata ttctaaaaaa
    26161 ggggtgtgca tcatgcacaa tgagaaatta attaaaggct tatatgacta tcgtgaggaa
    26221 catgatgcgt gtggtattgg tttttatgcg aatatggata ataaaaggtc tcacgacatc
    26281 attgataaat cgcttgaaat gttgcgacgc ttagatcaca ggggcggggt cggcgcagat
    26341 ggcatcactg gtgatggcgc aggtattatg actgaaatac cttttgcatt tttcaaacaa
    26401 catgtaacgg actttgatat cccaggtgaa ggtgaatatg ccgtggggtt atttttttcc
    26461 aaagaacgca ttttaggttc tgaacatgaa gtagttttta aaaaatattt tgaaggcgaa
    26521 gggttatcaa ttcttggtta tcgtaatgta ccagttaata aagatgccat tgctaaacat
    26581 gtagcagata cgatgccagt cattcaacaa gtgtttattg atattaggga cattgaagat
    26641 gttgaaaagc gtttgttttt agcgagaaaa caattagagt tctattcgac tcagtgcgat
    26701 ttagaattgt attttacgag cttatcacgc aaaacaattg tatataaagg ttggttacga
    26761 tcagaccaaa ttaaaaaact atatacagat ttatcggatg atttatatca atcaaagcta
    26821 gggttagtgc attcgagatt tagtacgaat acattcccga gttggaaaag ggcacatcct
    26881 aaccgtatgt taatgcataa tggtgagatt aacacgatta aaggtaatgt aaactggatg
    26941 cgagcacgcc aacataaatt aatcgaaaca ttatttggcg aggatcaaca taaagtgttt
    27001 caaattgtcg atgaggatgg tagtgactct gccattgtag ataatgcgct agagttctta
    27061 tcgttagcca tggagccaga aaaggcagcg atgttactca tacctgaacc ttggttatat
    27121 aatgaagcga atgatgcaaa tgtacgtgcg ttttatgaat tttatagtta tttaatggaa
    27181 ccgtgggatg gtcctacaat gatttcgttc tgtaacggtg acaaacttgg cgcgcttaca
    27241 gatagaaatg gattacgtcc aggtcgttat acgattacta aagataactt tattgtcttt
    27301 tcatctgaag tgggtgttgt ggacgtacct gaaagtaatg ttgcttttaa aggtcaattg
    27361 aatcctggaa agttattgct tgttgatttt aaacagaata aagtcattga aaataatgat
    27421 ttaaaaggtg cgattgctgg agaattacca tataaagcgt ggattgataa ccataaagtt
    27481 gactttgatt ttgaaaatat acaatatcaa gattcgcaat ggaaagatga gacgttattt
    27541 aaattacaac gtcagtttgc atacacgaaa gaagagattc ataagtatat tcaggaactt
    27601 gtagaaggta agaaggatcc tatcggtgca atgggatatg atgcgccaat tgcagtgttg
    27661 aacgagcgac cagaatcact atttaattac tttaaacagc tgtttgcaca agttacgaat
    27721 ccaccaattg atgcgtatcg tgaaaaaatc gtaacgagtg aactttctta tttaggtggc
    27781 gaaggtaact tactagcacc tgacgaaacg gttttagatc gtattcaatt gaaaaggccg
    27841 gtattgaatg aatcacactt agcagcgatt gatcaggaac attttaaatt aacttattta
    27901 tcaacggtat atgaagggga tttggaagat gcgttagaag cattaggccg agaagcagtg
    27961 aatgctgtaa agcaaggcgc tcaaattcta gtgttagatg atagtggatt agttgatagc
    28021 aatggctttg caatgccgat gttactcgca ataagtcatg tgcatcaatt acttattaaa
    28081 gcagatttac gtatgtctac aagtttagtc gctaaatctg gtgagacacg agaagtgcat
    28141 catgttgctt gtttactcgc atatggcgcg aatgcaattg tgccatacct agcgcaacgt
    28201 acagttgaac aactgacatt gacagaaggg ttacaaggca ccgttgtcga taatgttaag
    28261 acatatacgg atgtattgtc agaaggtgtc attaaagtaa tggctaagat gggaatttcg
    28321 acagtgcaaa gttatcaagg ggcacaaata tttgaagcga ttggcttgtc tcatgatgtg
    28381 attgatcgtt attttactgg gacacagtct aagttatctg gtatttcgat tgatcaaatt
    28441 gatgctgaaa ataaagcacg tcaacaaagt gatgataatt atcttgcatc aggtagtaca
    28501 ttccaatgga gacaacaagg tcaacatcat gcttttaatc cggaatctat tttcttattg
    28561 cagcacgcat gtaaagaaaa tgactatgcg caatttaaag catactctga agcggtgaac
    28621 aaaaatagaa cagatcacat tagacattta cttgaattta aagcatgtac accgattgac
    28681 atcgaccaag ttgaaccggt aagtgacatt gtcaaacgct ttaatacagg ggcgatgagt
    28741 tatggatcga tttcagcgga agcacatgaa acgttagcac aagccatgaa ccaattaggt
    28801 ggaaagagta atagtggtga aggtggcgaa gatgcaaaac gttatgaagt acaagttgat
    28861 ggaagcaaca aagtaagtgc gattaaacaa gttgcttctg ggcgttttgg tgtaactagt
    28921 gattatttac aacatgccaa agaaattcaa attaaagttg cgcaaggtgc aaagcctggt
    28981 gaaggtggtc aattacctgg tactaaggta tatccgtgga ttgcgaagac aagagggtca
    29041 acgccaggta tcggtctgat ttcaccaccg ccacatcatg atatttattc aatagaagat
    29101 ttagcgcaac tgatacatga tttgaaaaat gcgaataaag atgcagatat cgcggtaaaa
    29161 ttagtttcga aaacaggtgt tggtaccatt gcatctgggg tggcaaaagc atttgcagat
    29221 aaaattgtca tcagtggtta cgatggtggt acaggggctt cacccaaaac gagtattcag
    29281 catgccggtg ttccttggga gattggttta gcagaaacac atcaaacatt aaaactaaat
    29341 gacttaagaa gtcgtgttaa gttagaaaca gacggtaagt tattaactgg taaagatgta
    29401 gcgtacgcat gtgcgcttgg agcggaagaa tttggatttg caactgcacc attagtggtg
    29461 ttgggctgta ttatgatgcg tgtatgccat aaagatacat gtccagtagg agttgcaact
    29521 caaaacaaag atttacgtgc tttatataga ggtaaagcac atcatgttgt taattttatg
    29581 cattttattg cacaagaatt aagagaaatt ttagcatctt taggtttgaa acgtgtagaa
    29641 gacttagttg gaagaactga tttattacaa cgatcatcaa cattaaaagc gaatagcaaa
    29701 gcggctagta ttgatgttga aaaactgtta tgtcctttcg atgggccaaa cacaaaagaa
    29761 attcaacaaa atcataatct tgagcatgga tttgatttaa caaatttata tgaagtaacg
    29821 aagccatata ttgctgaagg gcgtcgctat acaggtagct ttacagtaaa taatgaacaa
    29881 cgtgatgtag gggttattac aggtagtgag atttcgaaac aatatggaga agcaggactt
    29941 cctgaaaata caattaatgt ttatacgaat ggtcatgctg gtcaaagtct tgcagcatat
    30001 gcaccgaaag gcttaatgat tcatcatact ggagatgcga atgactatgt tggtaaagga
    30061 ttatctggtg gtacggtcat tgtcaaagca ccttttgaag aacgacaaaa tgaaattatt
    30121 gctggtaacg tctcattcta tggtgcgaca agtggtaagg catttattaa cggtagtgca
    30181 ggagaaagat tctgtattag aaatagtggt gtagatgttg tcgttgaagg tatcggcgac
    30241 catggattag agtatatgac tggtggacat gtcattaatt taggtgatgt aggtaagaac
    30301 ttcggtcaag gtatgagtgg tggtattgct tacgttatcc cgtctgatgt agaagctttt
    30361 gttgaaaata atcaactaga tacgctttcg tttacaaaga ttaaacacca agaagaaaaa
    30421 gcattcatta agcaaatgct ggaagaacat gtgtcacaca cgaatagtac gagagcgatt
    30481 catgtgttaa aacattttga tcgcattgaa gatgtcgtcg ttaaagttat tcctaaagat
    30541 tatcaattaa tgatgcaaaa aattcatttg cacaaatcat tacatgacaa tgaagatgaa
    30601 gcgatgttag ctgcatttta cgatgacagt aaaacaatcg atgctaaaca taaaccagcc
    30661 gttgtgtatt aaggaaaggg ggagatacga tgggtgaatt taaaggattt atgaagtatg
    30721 acaaacagta cttaggtgaa ttatcactgg tagaccgttt gaagcatcat aaagcatatc
    30781 aacaacgatt tactaaagaa gatgcctcta tccaaggtgc acgatgtatg gattgtggaa
    30841 cgccgttttg tcaaaccgga caacagtatg gtagggaaac aataggttgt ccaattggaa
    30901 actacattcc tgaatggaac gacttagtgt atcatcaaga ttttaaaact gcttatgaac
    30961 gcttaagcga aacaaataac tttcctgact ttacagggcg tgtatgtcct gcaccatgcg
    31021 aaagtgcttg tgtgatgaag attaatagag aatcgattgc gattaaaggt attgaacgca
    31081 caattattga tgaagctttt gaaaatggtt gggtagcgcc gaaagttccg agtcgccgta
    31141 gagatgaaaa agtggcaatc gttggaagcg gtccagcagg attagctgct gctgaagaac
    31201 ttaatctact aggatatcaa gtaactattt atgaacgtgc tagagaatca ggcggtttat
    31261 taatgtgtgg tattccgaat atgaaacttg ataaagatgt ggttcgacgt cgtattaagt
    31321 taatggaaga agcgggcatt actttcatta atggtgttga agtcggcgtt gatattgata
    31381 aagcaacgtt agaatctgag tatgatgcca ttatattatg tactggtgca caaaaaggta
    31441 gagatttacc tttagaagga cgcatgggtg atggtataca tttcgctatg gattatttaa
    31501 ctgaacaaac gcagttgtta aatggagaaa ttgatgatat aacaataact gcaaaagata
    31561 agaatgtcat tatcattggt gctggtgata caggggcaga ctgtgtagcg acagcattaa
    31621 gagaaaattg taaatcgatt gttcaattta ataaatatac gaaattgcca gaagcaatta
    31681 catttacaga aaatgcatca tggcctttag caatgccggt gtttaaaatg gactatgcgc
    31741 accaagagta cgaagctaag tttggtaagg aaccacgtgc atatggtgtt caaacaatgc
    31801 gttacgatgt tgacgataaa ggacacatac gtggtttgta tactcaaatt ttagagcaag
    31861 gcgaaaatgg tatggtcatg aaagaaggac ctgaaagatt ttggcctgct gaccttgtat
    31921 tattatcaat cggcttcgaa ggtacagaac caacagtacc gaatgctttt aacattaaaa
    31981 cggatagaaa tcgaatcgtg gcggatgata caaactatca aactaataat gaaaaggtat
    32041 ttgctgctgg agatgctaga cgtggtcaaa gtttagttgt atgggcaatt aaagaaggta
    32101 gaggcgtagc gaaagcagta gatcagtatt tagctagtaa agtttgtgta taatctttgt
    32161 atggaaatgg tggttacgtt gacgttgtga catgctgaat cgagtttgaa aaaatctagt
    32221 atctatcaac gtcacatgcc atctttgtaa cctaaaaaca aaggtttgta agacaacaaa
    32281 tagattaatt ataagtagtg attttttaca ttcgtttata ggtcaactgt agtggaagac
    32341 aatgatttgt ggtaatcatg taatgcttaa aaacaatatt gacttttaca gaacgttcat
    32401 atatgataaa tattgtgttt
//
LOCUS       DALAAR010000028        32213 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000028 DALAAR010000000
VERSION     DALAAR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.028"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..771
                     /locus_tag="O2458_001319"
     CDS             <1..771
                     /locus_tag="O2458_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ3887186.1"
                     /translation="FFSSYYSQINDWLNSLPKKIPSMLSEFTDGFGSKIATFAETIAN
                     IGVVIVTTPFVLFFMLKDGHHFKEFSTNIMPPKFRKDFHDLLEKMSVQVGSYIQGQII
                     VSFCIGILLFIGYSVIGLKYSLVLASIAAVTSVVPYLGPTIAISPAIVIAAITSPWML
                     LKLAVVWTLVQFVEGHFISPNIMGKTLKIHPLTIIFILLCAGKLLGIVGVILGIPGYA
                     ILKVLVTHLFQLFKRRYNRFYGNDVGEYDIKESNKIVE"
     gene            1252..3774
                     /gene="mprF"
                     /locus_tag="O2458_001320"
     CDS             1252..3774
                     /gene="mprF"
                     /locus_tag="O2458_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCZ3887187.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(3977..4486)
                     /gene="msrA"
                     /locus_tag="O2458_001321"
     CDS             complement(3977..4486)
                     /gene="msrA"
                     /locus_tag="O2458_001321"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ3887188.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            4623..5606
                     /locus_tag="O2458_001322"
     CDS             4623..5606
                     /locus_tag="O2458_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ3887189.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(5694..5879)
                     /locus_tag="O2458_001323"
     CDS             complement(5694..5879)
                     /locus_tag="O2458_001323"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCZ3887190.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            6027..7289
                     /locus_tag="O2458_001324"
     CDS             6027..7289
                     /locus_tag="O2458_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCZ3887191.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(7426..8517)
                     /locus_tag="O2458_001325"
     CDS             complement(7426..8517)
                     /locus_tag="O2458_001325"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCZ3887192.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            8682..9713
                     /locus_tag="O2458_001326"
     CDS             8682..9713
                     /locus_tag="O2458_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ3887193.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            10205..11611
                     /locus_tag="O2458_001327"
     CDS             10205..11611
                     /locus_tag="O2458_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCZ3887194.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            11608..12174
                     /locus_tag="O2458_001328"
     CDS             11608..12174
                     /locus_tag="O2458_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ3887195.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            12180..13178
                     /gene="trpD"
                     /locus_tag="O2458_001329"
     CDS             12180..13178
                     /gene="trpD"
                     /locus_tag="O2458_001329"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCZ3887196.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            13180..13962
                     /gene="trpC"
                     /locus_tag="O2458_001330"
     CDS             13180..13962
                     /gene="trpC"
                     /locus_tag="O2458_001330"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCZ3887197.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            13962..14594
                     /locus_tag="O2458_001331"
     CDS             13962..14594
                     /locus_tag="O2458_001331"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCZ3887198.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            14587..15801
                     /gene="trpB"
                     /locus_tag="O2458_001332"
     CDS             14587..15801
                     /gene="trpB"
                     /locus_tag="O2458_001332"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCZ3887199.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            15794..16522
                     /locus_tag="O2458_001333"
     CDS             15794..16522
                     /locus_tag="O2458_001333"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCZ3887200.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            16844..18106
                     /gene="femA"
                     /locus_tag="O2458_001334"
     CDS             16844..18106
                     /gene="femA"
                     /locus_tag="O2458_001334"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCZ3887201.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            18125..19384
                     /gene="femB"
                     /locus_tag="O2458_001335"
     CDS             18125..19384
                     /gene="femB"
                     /locus_tag="O2458_001335"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCZ3887202.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            20073..20840
                     /locus_tag="O2458_001336"
     CDS             20073..20840
                     /locus_tag="O2458_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ3887203.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(20888..21361)
                     /locus_tag="O2458_001337"
     CDS             complement(20888..21361)
                     /locus_tag="O2458_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCZ3887204.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(21485..22186)
                     /locus_tag="O2458_001338"
     CDS             complement(21485..22186)
                     /locus_tag="O2458_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887205.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(22179..22952)
                     /locus_tag="O2458_001339"
     CDS             complement(22179..22952)
                     /locus_tag="O2458_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887206.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(22939..23769)
                     /locus_tag="O2458_001340"
     CDS             complement(22939..23769)
                     /locus_tag="O2458_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887207.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(23762..24748)
                     /locus_tag="O2458_001341"
     CDS             complement(23762..24748)
                     /locus_tag="O2458_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887208.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(24919..25104)
                     /locus_tag="O2458_001342"
     CDS             complement(24919..25104)
                     /locus_tag="O2458_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887209.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(25052..25396)
                     /locus_tag="O2458_001343"
     CDS             complement(25052..25396)
                     /locus_tag="O2458_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887210.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            25601..27415
                     /gene="pepF"
                     /locus_tag="O2458_001344"
     CDS             25601..27415
                     /gene="pepF"
                     /locus_tag="O2458_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ3887211.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            complement(27555..28196)
                     /gene="phoU"
                     /locus_tag="O2458_001345"
     CDS             complement(27555..28196)
                     /gene="phoU"
                     /locus_tag="O2458_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCZ3887212.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(28203..29054)
                     /locus_tag="O2458_001346"
     CDS             complement(28203..29054)
                     /locus_tag="O2458_001346"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887213.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            complement(29101..30018)
                     /gene="pstA"
                     /locus_tag="O2458_001347"
     CDS             complement(29101..30018)
                     /gene="pstA"
                     /locus_tag="O2458_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCZ3887214.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            complement(30020..30946)
                     /gene="pstC"
                     /locus_tag="O2458_001348"
     CDS             complement(30020..30946)
                     /gene="pstC"
                     /locus_tag="O2458_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCZ3887215.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            complement(31137..32120)
                     /locus_tag="O2458_001349"
     CDS             complement(31137..32120)
                     /locus_tag="O2458_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCZ3887216.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
BASE COUNT        11150 a         4931 c         5425 g        10707 t
ORIGIN      
        1 tttttctctt cgtattatag tcaaattaat gattggttaa attctttacc taagaaaata
       61 ccatctatgt taagtgaatt tacagatggc tttgggtcta aaattgcaac gtttgcagaa
      121 acgattgcta atattggcgt tgtgattgtc acaacaccat ttgtactatt ctttatgctt
      181 aaagatggac atcacttcaa agaattttca acgaatatta tgccaccgaa attccgaaaa
      241 gattttcatg atctacttga aaaaatgagt gttcaagttg gttcatacat tcaaggacaa
      301 attatcgttt cattctgtat cggtatactg ttgtttatcg gttattcggt tatcgggttg
      361 aaatatagct tagtattagc tagtattgcg gcagttacaa gtgttgtacc atatttaggg
      421 cctactatag cgatttctcc agctattgta atagctgcta taacatcgcc gtggatgctc
      481 ttaaaattag cagtagtatg gactttagta caatttgttg aagggcactt catttcacca
      541 aatatcatgg gtaaaacact taagattcat ccacttacaa tcattttcat tttactgtgt
      601 gcaggcaaat tgcttggtat tgtaggcgtt attttaggta ttccgggata tgctatttta
      661 aaagtattag ttactcattt attccaatta tttaaacgtc gatacaatcg tttctatggt
      721 aatgatgtag gtgaatatga tattaaagaa agtaataaaa tagttgaata agtaataaaa
      781 aataccaatg actatatgtt tgttggtatt ttttatggtg cttataatgt tgggcagtta
      841 catttatgat tatcagcaca tgtttagctt ataaattttt acgaaagttt agccgtttat
      901 aaagcacatg cataatgaaa cgagtatttg ccacttgatt agtacttcat tattatgtcg
      961 aaaataaaaa taagtggtat ttttaatata ttaagaagca ctcataatcg gctgttaatt
     1021 aataatattt ttcataagta ttgattcatc attttcttta tgttaaatat gaatcagtat
     1081 agataaccat attgttctgt ttgagataga aatattttct atttacttct taatacagat
     1141 atcaatatga caaaagtgtt atataaatca aaggtaaatg atatgctatt tttatggttt
     1201 atatgatata ttttgattta taacagaaat aattagaatt gatgtgaaaa aatgaatcag
     1261 gaagttaaaa acaaaatatt ttcaatctta aaaattacgt ttgctacagc tttatttatt
     1321 tttgtagcaa tcacattgta tcgggagtta tctggtatta actttaaaga tacgttggtt
     1381 gaatttagta agattaaccg tatgtcctta gtgttactat ttattggtgg tggggcatcg
     1441 cttgttattc tatcaatgta tgatgtgatt ttatctagag ctttaaaaat ggatatatcc
     1501 ttaggcaaag ttttaagagt aagttatatc atcaatgcat tgaatgcgat tgtaggtttc
     1561 ggtggcttta ttggtgcagg cgttagagca atggtttata aaaactatac gcatgataaa
     1621 aagaaattag ttcactttat atccttaata cttatttcaa tgttgacagg tttaagctta
     1681 ttatcattgc taattgtatt ccatgttttc gatgcatctt taatcttaga taagattaca
     1741 tgggtaagat gggtattata tgtagtgtca tttttcttac cattattcat tatttattca
     1801 atggttagac cacccgataa aaacaatcgt tttgtaggat tgtactgcac tttagtgtcg
     1861 tgtgttgaat ggttagcagc tgcagttgta ttatatttct gtggtgtaat tgttgacgct
     1921 catgtatcat tcatgtcctt tattgcaata tttatcattg ctgcattatc aggtttagtc
     1981 agctttattc ctggtggttt cggcgctttc gatttagttg tattactagg atttaaaact
     2041 ttaggtgtcc ctgaggaaaa agtattatta atgctacttc tatatcgttt tgcgtactat
     2101 tttgtaccgg taattattgc attaatttta tcatcatttg aatttggtac atcagctaag
     2161 aagtacattg agggatctaa atactttatt cctgctaaag atgttacgtc atttttaatg
     2221 tcttatcaaa aggatattat tgctaaaatt ccatcattat cattagcaat tttagtattc
     2281 tttacaagta tgatcttttt tgtaaataac ttaacgattg tttacgatgc tttatatgat
     2341 ggaaatcact taacgtatta tattctattg gcaattcata ctagtgcttg tttattactt
     2401 ttactgaatg tagttggtat ttataagcaa agtagacgtg ccattatctt tgctatgatt
     2461 tcaattttat taatcacagt ggcgacattc ttcacttacg cttcatatat tttaataaca
     2521 tggttagcta ttatttttgt tctgcttatt gtagctttcc gtagagcacg taggttgaaa
     2581 cgcccagtaa gaatgagaaa tatagttgca atgcttttat tcagtttatt tattttatat
     2641 gttaaccata tatttattgc tggaacgtta tatgcattag atatttatac gattgaaatg
     2701 catacatctg tattgcgcta ttacttctgg cttacgattt taatcatcgc tatcatcata
     2761 ggtatgattg catggttgtt tgattatcaa tttagcaaag tacgtatttc ttctaaaatt
     2821 gaagattgcg aggagattat taatcagtac ggcggtaatt atttgagtca cttgatatat
     2881 agtggtgaca agcagttttt cactaatgaa aataaaacag catttttaat gtatcgttat
     2941 aaagcaagtt cattagtggt tcttggagat ccgttaggtg atgaaaatgc ctttgatgaa
     3001 ttgttagaag cattctataa ttacgctgag tatttaggct atgatgttat attctatcaa
     3061 gttacagatc aacacatgcc tttatatcat aatttcggta accaattttt caaattaggt
     3121 gaagaagcaa ttattgattt aacgcaattt tcaacttcag gtaaaaaacg ccgtggattt
     3181 agagcgactt taaataaatt cgatgaactt aatatttcgt tcgaaattat tgaaccaccg
     3241 ttttcaactg aatttataaa tgaacttcaa catgtaagtg atttatggct agataatcgt
     3301 caggaaatgc atttctctgt tggtgaattt aatgaagaat acttatctaa agcgccaatt
     3361 ggtgtaatgc gaaatgaaga aaatgaagta attgcatttt gtagtttaat gccaacatac
     3421 tttaatgatg ccatttcagt cgatttaatt agatggttgc cagagttaga tttaccatta
     3481 atggatggtc tatacttgca tatgttactt tggagtaaag aacaaggtta tacaaaattt
     3541 aatatgggta tggcaacgtt atcgaacgtt ggtcaattgc attattcata tttaagagaa
     3601 cgacttgcag gccgtgtctt tgaacatttc aacggtctat atcgtttcca aggattacgt
     3661 cgttataaat ctaaatataa tccgaattgg gaaccacgct ttttagttta tcgtaaagat
     3721 aattcgcttt gggaatcact ttctaaagta atgcgtgtaa tacgtcacaa ataattaaaa
     3781 tccaagtgct aagaggtata cagttatgcc tgatgcgctt ggattttttg gtttctataa
     3841 agaatgattt ataaaaaacg gtacagataa gaatatttaa gtttcctatc tgtaccgtat
     3901 tttaatattg aaatgagcta aagttaagtc gcctaacttt tattatcagt ccaatcagtt
     3961 tcatcaagta taaatgttat tgcttatttt tgtattcttg gcgtattttt tgttcttctg
     4021 catagcgctc tggatttttc ttataaaaat cttggtgata gtcttcggct ttgtaaaatt
     4081 gtgacgctgg taatattttt gttgcaattg ccttatcagc attaatcgta tttttaagct
     4141 gctcgatata agtctcagcg agttcttttt gatgatcatt agtgtagaaa atagctgttt
     4201 gatattgagg accacggtct tgatattgac cacctgtatc taatgggtca atgactgaga
     4261 aaaatatttc taataactta ttgtatgaga ataatgcaac atcatattga atttcaacag
     4321 tttctaaatg accactcgta cctgatttta cttgttcgta agtaggattt tcaatatgtc
     4381 cgcccatata tccagaagtt actttttcta tgccgtcaaa ggtgtcaaat ggtttcgtca
     4441 tacaccaaaa gcaacctccg gcaaaataag ctgtattaat attcattttt gacatccttt
     4501 cattagacct tagtacgatt tattaagaaa tcacttgctt tttgaattgt ttttatataa
     4561 cgttaatatg tgattattat agtacacaaa aatctatttt tgaagatata aggtaggtaa
     4621 atatggataa agaaactaat gacaacgaat atagacgtca aagtgaacat cgcacttcgg
     4681 cgcctaagcg aaaaaagaag aagaaaatta ggaaattacc tatcattctt ctgattgttg
     4741 taattttact tatcgcatta gttgtatata ttgtacatag ttacaatagc ggtgtagaat
     4801 atgccaagaa acatgcgaaa gatgttaaag tacatcaatt taatggacca gtaaaaaatg
     4861 atggtaaaat ttctattctt gtactcggtg cagataaagc acaaggtgga caatcaagaa
     4921 cagattctat catggttgtt caatatgact ttatcaataa aaagatgaaa atgatgtctg
     4981 tcatgcgtga tatttatgca gatattccag gatatggaaa acacaaaatt aattcagcat
     5041 acgctttagg tggtccagag ctacttagaa aaacacttga taaaaattta ggaattaatc
     5101 ctgaatatta tgcagtagtt gattttactg gatttgagaa aatgattgat gaattaatgc
     5161 ctgaaggtgt accaattaat gtcgaaaaag atatgtcgaa aaatattggt gtatctttga
     5221 aaaagggtaa ccataggttg aatggtaaag aattacttgg ttatgcaaga ttccgtcacg
     5281 accctgaagg tgacttcgga cgcgtgcgac gtcagcaaca agtgatgcaa acattgaaaa
     5341 aagaaatggt taattttaga acagttgtta aattaccaaa agttgcaggt attttaagag
     5401 gctatgtgaa tacaaacatt cctgattcag ggattttcca aacaggtttg agttttggta
     5461 tccgaggtga aaaagatgtt aagtcattga ctgtgccaat caagaactca tacgaagatg
     5521 tcaatacaaa tactgatggt agtgcattac agattaataa aaacacaaat aaacaagcta
     5581 ttaaagactt tttagatgaa gattaaaaat aaacaaggcg atttctatca tactcagata
     5641 gaagtcgcct tgttcgtttt caaagaatta attggcatgc atcttatgaa gaattattga
     5701 tctgactttc ttacgccagc cacaccataa tggtttggtt tcatttcttc tataacaacg
     5761 tgaattgctt gtctatttgc ccccgttgtt ttttctacgg cgtcagttac ttcgctaact
     5821 aaatttttta attgttcatc cgaacgacct tctaataatt ttacattgac gattggcatc
     5881 atccaaacct cctaatcatt attaatttga gtatatcatg taatacttaa aaattcattt
     5941 gcaaaataac agaacatttg ttctaaaata gttgaaatag aacacgtgtt cgtatataat
     6001 aagattcaag taagggagga ataacagtgt ataattatca tttattagaa gatagggatg
     6061 ttctatgtat tgaccaaaaa agtttttttg cgagtgtttc ttgtattgaa aaggggctag
     6121 atccattaga aacaaagcta gctgttgttg cagatactaa gcgtcagggt tctgtaatat
     6181 tggctgcgac acctaaatta aaagaattag gcatcaagac agggtcgcga ttgtttgaaa
     6241 taccacatag aaatgatatt tacattatca atccaagtat gcgtaaatat cttaatgttt
     6301 cagttgctat ttctaagatt gcattgcgtt atattccacc tgaagattta caccaatata
     6361 gtattgacga attttttatg gatgttactg atagctatca tagatttagt tctacagtac
     6421 atgcattttg cgaaagactt aaacgtgaaa tttatgaaga aacaggcatt tattgtactg
     6481 tgggcattgg ttctaatatg ttattaagta aaattgctat ggatgttgaa gcgaagcata
     6541 gtcaaaatgg tatagctgaa tggcgatatc aagatgtacc aacgaaatta tggccaattc
     6601 agcccttgcg agatttttgg ggtattaatc gtcgaacaga agccaaattg aataaaagag
     6661 gaatttttac tataggagat ttagcgaaat atccatataa atttttaaaa aaagagttcg
     6721 gtattttagg tgttgatatg catctacatg cgaatgggat agatcagagt aaagtacgtg
     6781 aaaagcacaa gatcagcaat ccatcgatat gcaaaagtca aatattaatg agagattatc
     6841 attttgatga agcaaaagta gtaatgcaag agttaattga agatgttgct agcagagttc
     6901 gagcaagaaa aaaagtggca agaacgatac attttgcctt tggctatagt gatgaaggcg
     6961 gtgtacataa gcaatatact ttgaaagatc caacaaactt agaaaaagat atttataaag
     7021 tagtaatgca tttcgcagat aaattatgta ataaacaagc actatatcgt acgctaagta
     7081 tatctttgag tcaatttatt aatgaggatg agcgacagtt aagtctgttt gaagatgaat
     7141 accaacgcaa acgtgacgaa tgtctagcta aaacgataga ccaattacat ttgaaatacg
     7201 gcaaaggtat tgtgtccaaa gcagtatcgt ttacagaagc aggtacaaaa cacggcagat
     7261 taggtttaat ggctggacat aaaatgtaat gactatacgg tttaagtaat atataactgt
     7321 gattcgtata aaataagtct ctaaagataa atatttcata tatcacaata gattttcaca
     7381 ataatatcta agaatacatg gaatttatca aaagagactt aataattatt ggatataaca
     7441 atcaaaatca ctcaatgctt gcataccgcg ttctcggtca gtagggtttt tgaaactaat
     7501 ttttaaagca ccgtatatat cttcgcgtac ttctaagatt cttaagttgc ttatagatat
     7561 gttatgtaaa ctcaggatat aagtcacttt acttatcata cctgattcat ccggaatgtc
     7621 tacatataga tcatacgcag tatttagtcc acctagttgt ttagcgggta gtgcgtcgcg
     7681 atacgattta gcttgggcaa aaaatgataa caatttttca gaatcattgc tttcaattag
     7741 tctttctaaa tcttgaaact gactttttag ctgtcgaatc atttctaaaa tatacgtttt
     7801 attactcaag gtgatatctt tccacatttg tgcattacta ctagctatac gagtgatatc
     7861 acgaaaacca ccagctgcaa gtttattaac taaatgatgt tcttgaccgt tcttttgact
     7921 aacatgaact aaactagatg caacgatatg aggtaaatga cttacgacgc ttgttacgta
     7981 gtcgtgttct tcagcagtag ttacaataaa tttagcaaga gtaggtgata acagttcttt
     8041 taacgtgttt gctgcttgct catttcttgg ctcattgtag actaaaatat aataagcgtt
     8101 ttcaaataag tgctttttag catttagtac accagattta tgactaccag ccattggatg
     8161 accactgact aaatgaatat tatgctttaa taaattgcat tcgtgttgct gtatcattgc
     8221 tttagtacta ccagtatcag aaacaataac accaggttta gttggcatat ctataagctc
     8281 gctaagatat ttatttgtga tagcaacagg tgttgcataa ataattacat cggctttttt
     8341 aatagcttca ctataattta aacatttttc attaataatg ccgattgatt tagctttatc
     8401 taactgagaa gtatctgcat cgtatgcaat aatattagta ttagggttat ggtattttat
     8461 attgctagca agacttccac caattaatcc aagcccaaca aataaaactg ttgtcatata
     8521 aatcacctta tttcgaaatt ttcagaataa taacattgta aatgagctgt tgacacagtg
     8581 caatagtaaa taaaaatcga taatagcatt aatagataaa cggagataaa tcatctacaa
     8641 taaagagtat agtaacacaa tggcaacgga ggggtaaatc aatggaacca atattagaaa
     8701 tgattaaaac attaacaggt attaatagtc cttcaggaga cacagaagaa gcaattcaat
     8761 ttgtcgaaaa atatgcaaaa gacttgggtt atcaaacaac actaacaaat aaaggtgcgt
     8821 tattaataac agtgccaggc aaaaatgatg aagtacaacg ctgtattact gctcatgttg
     8881 atactttagg tgcaatggtt aaagaaatta aagaagatgg tcgcttagca atagaattaa
     8941 ttggaggatt cacgtataac gcgattgagg gtgaatattg ccaaattaaa actgatgctg
     9001 gtcaaatata tacaggaaca atttgtctgc atgaaacaag tgttcatgta tatagaaata
     9061 atcatgaaat acctagagat caaaagcata tggaaataag aattgatgaa gtaactacat
     9121 cagaagaaga tacaaagagt ttaggtattt cagtaggtaa ttttgttagc tttgatccac
     9181 gtacagttat cacgtcatca ggttttatta aatctcgtca tttagatgat aaagctagcg
     9241 tagcgatgat actacaatta ctaaagaaat taaaagaaga gcaaataata ttaccacata
     9301 caacgcaatt ttatatttct aataacgaag aaataggtta cggtgcaaat gcatcaattg
     9361 attcgaaaat caaagaatat attgcattag atatgggcgc gttgggagac ggtcaagcat
     9421 cggatgaata tacagtttct atttgtgcca aagatgcttc aggtccatat cataagcaat
     9481 tgaaatcgca cctagttaat ctttgcaaaa taaataacat tccatataaa gtagacatat
     9541 atccatatta tggttcagat gcttcagcag ctttacatgc tggtgcggat atcagacatg
     9601 gtttatttgg cgctggcatt gaatcatctc atgcaatgga acgaacacat attgattcta
     9661 ttaaagcgac agagaaatta ctatatgcat attgcttatc accaattgag taaacaatta
     9721 gtgttgacaa atgtgaacga cctatgtaat ataatgaact ataaaaataa ttagaatttt
     9781 ctaaagaaat agtagcagat atgaaacgta gcaaatagaa agctaatggg tgatgggaat
     9841 tagcacgcca tatcttgtga attggacttt ggaaaacaat tgaatgagtt ttgaaagtga
     9901 acatgaatta tgttaactaa ggtggcacca cggtaacgcg tccttacagg tatatgcgtt
     9961 atgtggtgtc tttttattta gacaaaatgt agtagttaat taaaggtagc aacagaaagt
    10021 tagtggatga tgtgaactaa caccgagatt aatgaaattg ggttttgtct gcaacagaaa
    10081 aattatatat agtaaagagt gaactatgaa tatttcgaat attcggttaa tttaggtggt
    10141 accacgcgtc agcgtccttt atattgataa ggatgctggc gcttttttga aaggagcgta
    10201 tagaatggat atattttata aaaaaataaa agcaaatgta acgcccgaag ttttagcaca
    10261 acttcattcc aagaagatca ttttggaaag tacaaatcaa caacaaacta aaggtcgcta
    10321 ttcagttgtt atttttgata tttatggcac tttaacttta gataatgatg tattatcagt
    10381 aagtacttta aaagaatcgt atcaaatcac tgaaagaccg taccattatt taacgactaa
    10441 aataaatgaa gactaccata atattcaaga tgagcaactt aagtcattac catttatatc
    10501 tggatatgtt gggacgtgta gctttgattt agtaagacat gaatttccta aattgcaatc
    10561 aatacaatta gaagatcaca agcagcacga tgtaaggtta tatatggttg aacaagttta
    10621 tgtatttgac cattacaaag atgagttata tatcatcgcg acgaatcaat tttcaaattc
    10681 aacaaaatca gatcttgaga atcgagttat taagtctatc gaagacttaa ctaaaatcca
    10741 accattcatg cctacacaag attttgattt taaaactaaa gaaattcaat caaacatttc
    10801 tgaagaaaga tttatcgaaa tgattcagta tttcaaagag aaaataacag aaggggatat
    10861 gttccaagtt gtgccatcaa gaatttacaa atatgcgcat catgctagtc agcatttaaa
    10921 tcaactttcg tttcaactgt atcaaaattt aaaacgacaa aacccaagtc catatatgta
    10981 ttatcttaat atcgatcaac catatattgt cggtagttct cccgaaagtt tcgtaagtgt
    11041 caaagatcaa attgtaacaa ctaatcctat tgcaggtacg attcaacgtg gtgagacgac
    11101 acaaatagat aatgagaata tgaaacaact acttaatgat ccaaaagaat gcagcgaaca
    11161 tcgtatgcta gttgatttag gacgtaatga tattcataga gtaagtaaaa tcggtacctc
    11221 aaaaattact gaattaatgg ttattgaaaa atatgaacat gttatgcata tcgtaagtga
    11281 agtcacaggt aaaataaatc aaaatttatc gccaatgaca gttattgcga atttattacc
    11341 aacaggtacc gtttcaggtg caccaaaatt acgtgcaatt gaaagaatat atgaacaata
    11401 tccacataaa cggggcgttt atagtggtgg tgttggatac ataaattgta atcataactt
    11461 agattttgca ttagcaattc gaacgatgat gatagatgag cagtatatca acgtagaagc
    11521 tggttgtggc gttgtatatg attctattcc tgaaaaagaa ctgaatgaaa cgaaattgaa
    11581 agctaaaagc ttattggagg tgagcccatg atcttagttg tagataatta tgattccttt
    11641 acatataacc tagtggatat tgttgctcaa catactgacg tcattgttca ataccctgat
    11701 gatgataatg tgctgaatca atcggtggac gctgttatta tatctcctgg tccagggcat
    11761 ccattagacg atcaacagtt aatgaaaatc atatcaacct atcaacacaa acccatttta
    11821 ggtatttgtt taggggctca ggcactgact tgttactacg gtggagaagt cattaaaggc
    11881 gacaaggtta tgcacggcaa agttgataca ctaaaggtta tatcgcatca tcaacatctg
    11941 ttatatcaag atataccaga acagttttca attatgagat atcattcatt aataagtaac
    12001 cctgacaatt ttccagaaga attgaaaatt actggacgta ccaaagattg tatacagtca
    12061 ttcgagcata aagaaagacc gcattatggt attcagtacc atcctgaatc atttgctaca
    12121 gactatggtg tcaaaataat tacaaatttc attaatctag tgaaggaagg atgaaaacca
    12181 tgacattact aacaagaata aaaactgaaa ctatattact tgaaagcgac attaaagagc
    12241 taatcgatat acttatttct cctagtattg gaactgatat taaatatgaa ttacttagtt
    12301 cctattcgga gcgagaaatc caacaacaag aattaacata tattgtacgt agcttaatta
    12361 atacaatgta tccacatcaa ccatgttatg aaggggctat gtgtgtgtgc ggcacaggtg
    12421 gtgacaagtc aaatagtttc aacatttcaa cgactgttgc ttttgttgta gcaagtgctg
    12481 gcgtaaaagt tataaaacat ggtaataaaa gtattacctc aaattcaggt agtacggatt
    12541 tgttaaatca aatgaacata caaacaacaa ctgttgatga tacacctaac caattaaatg
    12601 aaaaagacct tgtattcatt ggtgcaactg aatcatatcc aatcatgaag tatatgcaac
    12661 cagttagaaa aatgattgga aagcctacaa tattaaacct tgtgggtcca ttaattaatc
    12721 catatcactt aacgtatcaa atggtaggcg tctttgatcc tacaaagtta aagttagttg
    12781 ctaaaacgat taaagattta ggtagaaaac gtgcaatcgt tttacatggt gcaaatggta
    12841 tggatgaagc aacactatct ggtgataatt tgatatatga attgactgaa gatggagaaa
    12901 tcaaaaatta cacattaaat gcgactgatt atggtttgaa acatgcgccg aatagtgatt
    12961 ttaaaggcgg ttcacctgaa gaaaatttag caatctccct taatatcttg aatggtaaag
    13021 atcagtcaag tcgacgtgat gttgtcttac taaatgcggg tttaagcctt tatgttgcag
    13081 agaaagtgga taccatcgca gaaggcatag aacttgcaac tacattgatt gataatggtg
    13141 aagcattgga aaaataccat caaatgagag gtgaataata tgacgatttt atcagaaatt
    13201 gttaaatata aacagtcact tttacaaaat ggctattatc aagacaaact taataccttg
    13261 aaaagtgtga agattcagaa taaaaaatct tttataaacg caattgagaa agaaccaaag
    13321 ctagcaatta ttgcagaaat taaatcgaag agtcctacag ttaatgactt acctgaacga
    13381 gatttatcgc aacaaatctc agattatgac caatatggtg caaatgccgt gtccatttta
    13441 actgatgaaa agtactttgg tggtagtttt gaaagattac aagcattgac gacaaaaaca
    13501 acattacccg tattatgcaa agactttatt atagacccgc ttcaaattga tgttgctaaa
    13561 caagctggtg catctatgat tttattgatc gttaacatct tatctgataa acaattgaaa
    13621 gatttatata actacgctat atcgcaaaat ctagaagtgt tagttgaagt acatgatcgc
    13681 catgaattag aacgtgccta taaggttaat gctaaattga ttggtgtaaa taacagggac
    13741 ttaaaacgat ttgttacaaa tgtggaacat acaaatacta ttttagaaaa taaaaaaaca
    13801 aatcattatt atatttctga aagtggtatt cacgatgcat ctgatgtaag aaaaatcttg
    13861 catagtggta tcgatggctt actaataggt gaggcgctta tgcgttgtga caatctatct
    13921 gaatttttac cacaactgaa aatgcaaaag gtgaagtcat gatgaaattg aaattttgtg
    13981 gctttacatc aataaaggat gttacagcgg ccagtcaatt acctattgat gcgataggtt
    14041 tcatccatta tgaaaaaagt aaaaggcatc aaacaattac ccaaataaaa aagttagcgt
    14101 ctgctgttcc aaatcatatc gataaagtat gtgtcatggt aaatcctgat ttaacaacaa
    14161 ttgaacacgt attaagcaat acgtcaatta acacaataca gttacacggc acagaatcta
    14221 ttgattttat acaggaaatt aaaaagaaat attcaagcat taaaatcact aaagctttag
    14281 ctgcagatga aaacataatc caaaacataa ataaatataa agggttcgta gatttattta
    14341 ttatcgacac accctcagtg tcgtatggtg gtaccggtca aacatatgac tggactattt
    14401 tgaagcacat aaaagacata ccttatttga tagcaggagg cattaactct gaaaatattc
    14461 aaacagttaa tcaacttaaa ttatcacatc aaggttatga tcttgcatca ggtatagaag
    14521 taaatgggcg aaaagatata gaaaaaatga cagcaattgt aaatattgtg aaaggagata
    14581 gagacaatga ataaacaaat acaaacagaa gcagatgaat taggtttctt tggtgaatat
    14641 ggagggcaat atgttccaga aacattaatg ccagcaatta ttgagttgaa aaaagcttat
    14701 aaagaggcaa aagcagaccc agagtttcaa agagaactgg aatactattt atcagagtat
    14761 gtaggacgcg cgacaccact tacatatgct gcatcatata ctgaaagcct aggtggcgct
    14821 aaaatatatt tgaaacgaga ggatctaaat catacaggcg cccataaaat taataatgcg
    14881 ttaggtcaag cgttgcttgc taaaagaatg ggcaagaaga agcttgttgc tgaaactggt
    14941 gcgggtcaac atggtgtagc tagtgctacg gttgctgcat tatttgatat ggaacttgtt
    15001 gtctttatgg gaagtgaaga tattaaaaga caacaactta atgtatttag aatggaatta
    15061 cttggtgcaa aggttgtggc agttgaagat ggtcaaggga ctttatcgga tgcagttaat
    15121 aaagcattgc aatattgggt aagtcatgta gatgatacac attatttatt aggttctgca
    15181 ttaggtccag acccgttccc aacgattgtt agagattttc agagtgtgat tggtaaagaa
    15241 ataaaatcac agatattgaa gaaagaaggt cgacttccgg atgcaattgt agcatgtatc
    15301 ggtggtggct caaatgcaat cggtacattt tatccattta ttaaagatga tgttgcatta
    15361 tacggtgttg aagccgcagg tcaaggcgat gatactgata aacatgcact tgcaattggc
    15421 aaaggatcac ctggcgtatt acatggtact aaaatgtatt taattcaaga tgaagatggg
    15481 caagtgcaac tagcacattc tatttcagca ggacttgatt atcctggtat tggaccagaa
    15541 cattcttatt accacgacat tggtagagta acttttgaaa atgctagtga tacacaagca
    15601 atgaatgctt taatcaactt tacaaaacat gaaggtatta tacctgcaat tgaaagtgca
    15661 catgcactga gttatgttga aagactagcg cctacgatgt cgaaagaaga tattattgta
    15721 gtaactattt ctggacgtgg cgataaagat atggaaacaa ttagacaata tatggtagag
    15781 cgaggtcttg caaatgacta aattatttat accttatatt atgggcaata aagatttgat
    15841 tgaaaatgca acattgttga gtgaaaatgg tgcagatata attgaaattg gagtaccttt
    15901 ctctgatccg gttgctgatg gtccagttat catggaagca ggtcaacaag cgattaaaca
    15961 aggcatcacg atagattata ttttcaatca attagaaaaa catggtgatc aaattaagtg
    16021 taactatgta ttaatgacgt attataatat tatttgtcat tatggagaac aagcgttttt
    16081 tgaaaaatgt cgagatactg gtgtctacgg cttaattatt cctgatttac catatgaatt
    16141 atcgcagcgt ttaaaacaac aatttagtca ctatggcgtc aaaatcatat cgttagttgc
    16201 gatgactact gatgacaaac gtataaaaga tatcgtatcc catgcggaag gctttattta
    16261 tactgtgacg atgaatgcga caacagggca aaacggtgcg tttcatccag aattaaaacg
    16321 aaaaattgag tcaattaaag cgatagccaa tgtgccagtt gtcgcaggat ttggtataag
    16381 aacaccacaa catgttgcag atataaaaga ggttgcagat ggcattgtca ttggtagcga
    16441 aatcgttaag cgatttaaat ctaacacgcg tgaggaaatc attaaatatt tacaatctat
    16501 ccaacaaaca ttgaataatt aagtttactt gatttaaaaa aattaggcga atactgtttg
    16561 aaaaagtgaa aaacggtgaa ttataaaatt gaatagaaat gatacaaaag taatatgagc
    16621 aaacccaaac gttcatatta ctttttttga aattgtattc aaaaatctaa atattactat
    16681 aaaagtatac gcaattaaag cgtttatgtt ttagttttaa cattaactat tgtatactta
    16741 tttagattag atttattatt tttgacattt gcagagggga aatagaaaaa ctgcaaatac
    16801 ggaaatgaaa ttaattaacg agagacaaat aggagtaatg ataatgaagt ttacaaattt
    16861 aacagctaaa gagtttggtg cctttacaga tagcatgcca tacagtcatt tcacgcaaac
    16921 tgttggccac tatgagttaa agcttgctga aggttatgaa acacatttag tgggaataaa
    16981 aaacaataat aacgaggtca ttgcagcttg cttacttact gctgtacctg ttatgaaagt
    17041 gttcaagtat ttttattcaa atcgcggtcc agtgattgat tatgaaaatc aagaactcgt
    17101 acactttttc tttaatgaat tatcaaaata tgttaaaaaa catcgttgtc tatacctaca
    17161 tatcgatcca tatttaccat atcaatactt gaatcatgat ggcgagatta caggtaatgc
    17221 tggtaatgat tggttctttg ataaaatgag taacttagga tttgaacata ctggattcca
    17281 taaaggattt gatcctgtgc tacaaattcg ttatcactca gtgttagatt taaaagataa
    17341 aacagcagat gacatcatta aaaatatgga tggacttaga aaaagaaaca cgaaaaaagt
    17401 taaaaagaat ggtgttaaag taagattttt atctgaagaa gaactaccaa tttttagatc
    17461 atttatggaa gatacgtcag aatcaaaagc ttttgctgat cgtgatgaca aattttacta
    17521 caatcgctta aaatattaca aagaccgtgt gttagtacct ttagcgtata tcaactttga
    17581 tgaatatatt aaagaactaa acgaagagcg tgatatttta aataaagatt taaataaagc
    17641 gttaaaggat attgaaaaac gtcctgaaaa taaaaaagca cataacaagc gagataactt
    17701 acaacaacaa cttgatgcaa atgagcaaaa gattgaagaa ggtaaacgtc tacaagaaga
    17761 acatggtaat gaattaccta tctctgctgg tttcttcttt atcaatccat ttgaagttgt
    17821 ttattatgct ggtggtacat caaatgcatt ccgtcatttt gccggaagtt atgcagtgca
    17881 atgggaaatg attaattatg cattaaatca tggcattgac cgttataatt tctatggtgt
    17941 tagtggtaaa tttacagaag atgctgaaga tgctggtgta gttaaattca aaaaaggtta
    18001 caatgctgaa attattgaat atgttggtga ctttattaaa ccaattaata aacctgttta
    18061 cgcagcatat accgcactta aaaaagttaa agacagaatt ttttaggaag ggaattatca
    18121 aaacatgaaa tttacagagt taactgttac cgaatttgac aactttgtac aaaatccatc
    18181 attggaaagt cattatttcc aagtaaaaga aaatatagtt acccgtgaga atgatggctt
    18241 tgaagtagtt ttattaggta ttaaagacga caataacaaa gtaattgcag caagcctttt
    18301 ctctaaaatt cctactatgg gaagttatgt ttactattcg aatcgtggtc cagtaatgga
    18361 tttttcagat ttaggattag ttgattatta tttaaaagag ttagataaat atttacagca
    18421 acatcaatgt ttatatgtta aattagatcc gtattggtta tatcatctat atgataaaga
    18481 tatcgtgcca tttgaaggtc gcgagaaaaa tgatgcccta gtaaacttgt ttaaatcaca
    18541 tggttacgag catcatggct ttacaactga gtatgataca tcgagccaag tacgatggat
    18601 gggcgtatta aaccttgaag gtaaaacacc cgaaacattg aaaaagacat ttgatagtca
    18661 acgtaaacgt aatattaata aagcgataaa ctatggtgtt aaagtcagat tccttgaacg
    18721 tgatgagttc aatcttttct tagatttata tcgtgaaact gaagagcgtg ctggatttgt
    18781 atcaaaaaca gatgattatt tttataactt tattgacaca tatggagata aagtattagt
    18841 accattagca tatattgacc ttgatgaata tgtgttaaag ttgcaacagg aattgaatga
    18901 caaagaaaat cgtcgtgatc aaatgatggc gaaagaaaac aaatcagata agcaaatgaa
    18961 gaaaattgca gaattagata agcaaattga tcatgatcag catgaattat tgaatgcaag
    19021 tgaattgagc aaaacggacg gcccaattct aaaccttgct tctggcgttt attttgcaaa
    19081 tgcatatgaa gtgaattatt tctctggtgg ttcatcagaa aaatataatc aatttatggg
    19141 accatacatg atgcattggt ttatgattaa ctattgcttc gataatggct atgatcgtta
    19201 taatttctat ggtttatcag gtgattttac ggaaaacagt gaagattatg gcgtataccg
    19261 ctttaaacgt ggatttaatg tacaaatcga agaattaata ggggatttct ataaaccaat
    19321 tcataaagtg aaatattggt tgttcacaac attggataaa ttacgtaaaa aattaaagaa
    19381 atagatgtaa atgatgttag ggcttatagt tattgatact ataggctctt ttttatatgt
    19441 tttttaataa agcctcgaat aatgatatat taaatgacaa gtttaacaag tgattgcaat
    19501 aaatataaat atgaattgca caacctattt gtaaatgatt gagttgagta tactttttaa
    19561 ttcattcaat atgactcaaa acaagccaat atgtattata tttttggtga ataaacctta
    19621 gcataacaat ttatgtctga ttatatatgt gtgtgtttgc aagtgtgtga tatcaaatgt
    19681 catatttgtc taattgtaat ttataaaatg catgagcaat gattgctgta acaaattcta
    19741 gcaaagtatt cagtttgtat tttagaaata aaaatgatga tagatgtatg atcgatataa
    19801 aagatttaat atgcaccaaa atgataacgt gagtaattgg tttaatgaaa cataacatga
    19861 atgtaaattt agcttactga cataaacact acacaaacat acagttatat aagtcccctt
    19921 atggaatttc acattctagt ttacataata tatattatag gaagttatat gtgtgtaacg
    19981 caaaaggtac cctacatcat aatcattatc taatatcgtc acataactta cttatgctat
    20041 aatcatggta ttatattgtt tggagtgatt tgatgagatt tgtctttgat attgatggta
    20101 cgctttgttt tgacggccga ttaattgacc agactattat tgatacattg ttacaattac
    20161 aacatgatgg tcatgaactt atatttgcat cagcacgtcc gattcgtgat ttgttgccag
    20221 ttttaccatc agtatttcat cagcacacat taattggcgc aaatggtgct atgatttcac
    20281 agcaatcaaa gatttctgtt atcaaaccaa ttcatactga tacatatcat catatcttca
    20341 aaataattca aaagtatgag ttagattata ttattgatga tgattggaat tatgctgcac
    20401 aacttgacgc tgagaacgcg atttttgagc gtttagatcc acataagctg gccagttgta
    20461 ttgatgttgc aaatatcgac acaccaatca agattatttt attaaatata gacccggcac
    20521 aaattacaac tatattagac gagctagata aataccatca agaattggaa atgattcacc
    20581 attcaaatga gtataacatt gatataacag cgcaaaatat taacaaatat actgcattac
    20641 aatatatatt tgatgcagat gttaaatata tagcatttgg taatgaccac aatgatattg
    20701 tcatgttaca acatgctagt agtggctata ttataggacc atcagaagca tacacacacg
    20761 caatattgaa acttgataaa atcaaacaca tcaataataa tgcacaagct atttgcaaag
    20821 tcttaaaatc atataaataa aaacacccct atcaaatgat aatcattatc aatcgatagg
    20881 ggctatttta ataaaattcg tcctcgaaca tttcttcctc ttcatctaat ccaaataatt
    20941 ctgccatttc tccatgttca attaacatgt ttaaatatgc atcgcggagt tcttcttcac
    21001 tcatatcatt aatcatttct ttaagactat caatccacat atttctgcgt aattgatagt
    21061 cttcttcaac ttcgtttaac atcattatat gtttatttgc tgcttctgga ctagctgtaa
    21121 agagtaatgc aatcatatgt ttacatatca ctcgtcttcc atcagcatga ggacaattac
    21181 atatggattt tctaggatgt tccatatcaa tataacaacg atatactttg ttgccactgc
    21241 cctttacttc agcctcatgc tgcgtttctg aaaatgattt taagttaatg acgcattcac
    21301 tttgataata attaaagcct ctttctatag aacgaatact tgcaatatca agtaatccca
    21361 ttaatgatac tcctttttat tattattttt aaataaagaa aataaaatag ataagtgtct
    21421 agattaaaat acttgattta tctatatttt ataacaagtc tagaattatc gcattcttaa
    21481 ataactaata tgaaaatgct tgcactaatt cttttgtata agggtgtcta tcaacattaa
    21541 ataattcctc tattgcaaaa tcatcgacta tcatgccatc cttaagaacg ataattctat
    21601 taactaagcg ttgtaacacg gataaatcat gagaaataac gataaaatga tttaagttcg
    21661 taatcgtttg cgcttttaat atattgatta cattttgttc agctataaca tctaaatttg
    21721 aagttatctc atcacatatt aaaacgcgag gctgtgctaa taacgaacgc atgacattaa
    21781 atctttgtaa ttgtccgcca ctcacttcgc ttggtaattt agtcaataat tgcgcgttta
    21841 actcaaaagt agataaatgt tgtaataata attgatcctg agcagtatta tcagttagac
    21901 ctctgtaata atataacgct tcttttaatg aggtctcaat cgtccaatca gggttaaagc
    21961 tagttaaagg gtgttggaaa atcggtaaca cagcattgtc acttaagtaa atctctcctt
    22021 taacaggttt aaacaagcca agaaccaatg aagcgagcgt acttttacca cagccacttt
    22081 cgcctaaaat accaacattt tctccatcag gtatagtaat attgatatct tgtagcacca
    22141 tctgcttttt attataacca aaagtcacat gttttaactc aatcatagta atccctcttt
    22201 aattgtgttc tatatttaat tagacgttca gtatacggat gcaaatgctc atacttgaaa
    22261 tgattaatat tacctcgttc aatgatttga ccttctttta aaacataaat gtactgacaa
    22321 tatttcaata catgacttaa gttatgtgtg ataataaata atgtttgacc atgttctaat
    22381 acaatatgct gtaataaatc catcacttga ttaccgttca aagcatccaa tgatgcaact
    22441 ggttcgtctg caatgattaa tttaggctcc aacatgagaa cgcttgctat gtatacgcgt
    22501 tcaagttggc ccccagaaag ttggaaacta tatttattta atatatcttt gctttgtaaa
    22561 ttaacccacg acaaagcctt atcaactttg gacaaagcct cttctttact acctttataa
    22621 tgcttacgat aaatcgcagt taactgttta cctaatttag tatggtcgtt aaaactttct
    22681 gcataatttt gagaaatata gccaattgta tgaccataat attgactcaa tctactaaca
    22741 ttttccccat caaattggta cgaatcatac gtgcagctta aatcaaatgg taaatattca
    22801 agtaaagctt tagcaatcaa actttttcca gcgccgctct ctccaatcaa ggcattaatc
    22861 tgttgactaa aaattttcaa atcaatccct ttaataagag atttctcact agtattcttt
    22921 attgttaaat tttgtatatc aatgagactc atcatattca ccccgttgtt tcagcaatct
    22981 atctcttagt gcatcaccgg ttaaattaaa aattaaaata gttatagcaa tgactgaagc
    23041 aggtgcaatc aacataattg gatgagacga aataaaatca cgaccttgtt gcaacatagc
    23101 gccccactct ggtgttggcg gttgtgcacc taacccaata aatgatagtg aacttatata
    23161 tagaatgatt ttaccgaaat caacgaccat caaaacgata atagccggta taattttagg
    23221 tgttaaatga cgtattaata ttgttcttgt tggtacatga aataattgtg ccatttttat
    23281 ataaggctta ttcatttcgc tattaactat acttctagtc aaccttgtgt aattcatcca
    23341 ttttattaat gtaattgaga taactaaatt ccataaagat ggttgaaaaa aacttgctaa
    23401 agcaatcatg atgataaatt ctggaatact tagaccaaca tcaataaacc ttaacactaa
    23461 tcgttcaatc cacccttttt tgtatccggc aaatagacct agtgtaacac ctatgacaac
    23521 gatagctatt aatgttaaaa cagtaacaaa caatgttgaa cgtgcaccga taataattcg
    23581 ggtaaataaa tctctcccat aatcatcagt tcctaataaa tgcaaccaac taataggttc
    23641 aaaagtttgt gataaattga ctttggttgc attttcacta ctgacaaaga attgcagtac
    23701 aattaccaca aaaataaatg caacgaatac aaaaaatatc aggttattct ttgaaaatat
    23761 tttatgcatg acggtcacta ctttctgata tcaatggtgt attggttttg atttttggat
    23821 ttcctaattg taaacgctgc ttcggatcaa gtaataacgt taataaatca gcaatcgtat
    23881 tgataataac aacgaagaag ccaataaata acacgcatcc ttgaataaca ggataatctc
    23941 gagatttaat actatccatt aatagataac caataccagg tatatcaaat aaattttcaa
    24001 tcactacagt accacctatt agactgccaa gtgaaatccc tagtaatggg ataatcggca
    24061 aaattgttgg ttttagtaaa tcatgaatta aaatataacg ttcattcata ccgcgtaatc
    24121 ttgatgcttg tacgatatta ctttgcaata acatcaataa attagaacgc actaaacgaa
    24181 tgatgtatgc acacatacct aaagatagcg tgattacagg taatataaac tgacttagta
    24241 taacgctatc tatattcatt aaatttgtga caataaataa taaaataata ccgataaaga
    24301 acgctggtaa actaatcgat agtgttgaga tcactctaat cactttatcc gtccacttat
    24361 gaaatcgttt ggctgctata atgccgagcg gtatagatat gcataacgac actactaatg
    24421 ttgaaaatga tatgagtaat gttatgggtg catagttgaa taatatctgt gttaccggtt
    24481 cttttgattc aaaacttttt cctaaattaa aatgtaataa atgattcatc caatgccacc
    24541 actgtaccaa taaagaatca tttaatccca atttatcttt ggttgcattt atttgttccg
    24601 tcgacacttg tgctacatca agatgtaata ttttatcaac aggattgcct ggtgataatt
    24661 tcattaaaat gaatgtaagt gtagaaataa caaataaaac aactatcatt tgcatcagtc
    24721 tatacaacat agactttatt atgaacataa tagtccccct ccttgtgtaa gttactaaca
    24781 ctttcttttt acatgagaat ggcgcatgta tatgcaactt acatattaag aactaacgtt
    24841 cattatagta ttatccataa agaaattgaa gtatatttaa ttttttaaca aaatcattat
    24901 aaaatataat attttgaatc aagtcaacca tgtaaaatat aaaaaagtca aaacaaaaac
    24961 aactatagca ctgtattcca tctctttcga aataattgtt actgcagtgt aacttaaaag
    25021 tcgatgattt tgtgcatata gttgtcgaat attatttttt atctttacgg cgaagttcag
    25081 cgccctcata gccgtatttt tcaatttgct tttctaattt acgcgctttt ctttctttac
    25141 gccaatttct agtaaaatac cataatagaa aactaattaa taaactcata atcgctaaaa
    25201 atgcagcgta tcctaataat ggttgatatt ttatatcttg aaaatttgga ataaaaaatg
    25261 caagcacacc taatataaca aatgtaatta ctgcagatac aaaccattta tttaaaacta
    25321 agcaacagaa tattgttaat aaaatcatta ttaatgttgt gatccataaa taattaggca
    25381 tatcgaataa tgtcatattc attctccttt tatttcatta ctttccttgt atacatttta
    25441 ttataaattt ttaaaaactt aaacaatagc agtcagtttc aagcaatatt ctatctacta
    25501 atagaaaaat cattgttcct tgcgacatgg aaatcgtaac attatcgttt aggagacaaa
    25561 attatgtata atgaatgtat tataccaaag gagtgattat atgtctcaag gtttaccttt
    25621 aagagaagat gttcctgttt cagaaacatg ggatttagta gacttattta aagatgatca
    25681 acaatattat gaaagtattg acgctctagt acaacaagca aatcaatttc atcatacata
    25741 tgcaacaaca ttaaattcaa tcgaacaaat taatactgct ttagctgaat tagaaaatat
    25801 tttaattgcc ttagatcgct taagtaatta tgcagaacta cgtttaagtg tagatactag
    25861 taatatcgag gcacaagtat tgagcgctaa attatctact acatacggta aaattgttag
    25921 ccaattatca tttgtagagt cagaaatact tgaattacca gaagaaatac ttcaacaatt
    25981 agaagaatca tgtccatatc aacactatat taaacagtta ataaaacaaa agccattcca
    26041 attatctgcg tcggtagaac aagtattagc aactttatca cctacgctaa acagtcctta
    26101 cgatttatac ggcacgacaa aaatgctaga tattacattc gattcatttg aacatgatgg
    26161 tacaacgtac cctgtcgact atgctacgtt tgaaaatgat tatgaagata ataaagatcc
    26221 tgagtttaga cgtaaaagtt tcaaatcgtt tagcgatggg attcgaaaat atcagcatac
    26281 taccgcggct acatataata tgcaagtaca acaagaaaaa attgaagctg atttacgtgg
    26341 atttgaatca gtcatcgatt atttattaca tagtcaagaa gtaacgcgtg atatgtttga
    26401 ccgtcaaatc gatatgatta tgcgtgactt ggcaccagtt atgcagaaat atgctaaact
    26461 tttacaacgt attcacggat tagataacat gcgttttgaa gacttgaaga tttctgtaga
    26521 ccctgattat gaaccagaga tttcaattga agactcaaaa aattatattt tcggtgcgtt
    26581 aagtgtttta ggtgatgact atacaaacat gttacgtgaa gcatacgatc agcgatggat
    26641 tgattttgca caaaataaag gtaaagatac aggcgcattt tgtgcaagtc catactttac
    26701 acattcatat gtgtttattt cttggactgg taaaatggct gaagcatttg tcttagcaca
    26761 tgaattaggt catgcaggtc attttacatt agctcaaaaa catcaaccat atcttgaatc
    26821 agaagcatca atgtactttg ttgaagcccc ttctacaatg aatgaaatgt tgatggccaa
    26881 ttatttattt aacacaagtg ataatccaag atttaagcgt tgggttattg gctcaatttt
    26941 atctagaaca tattatcata atatggttac ccatttatta gaagctgctt atcaacgtga
    27001 agtgtatcac aaagtagatc aaggtgaatc tttaaatgcg ccgacattaa atgaaataat
    27061 gctaaatgtt tataaacaat tttttggaga tgcagtagac atgactgagg gtgctgaatt
    27121 aacatggatg cgtcaacctc attactatat gggattatat tcgtatacgt attctgctgg
    27181 cttaacaatc ggaactgtcg tttctcaaaa gattaaaaat gaaggccaac cagctgttga
    27241 tgcttggtta gaaacattga aaaaaggtgg tagtgtatca cctgtcgaac ttgcaaacat
    27301 tgcaggtgta gacattacta cagaacagcc acttaaatct acaattcaat atatttctga
    27361 tttagtcgat gaagttgaaa aattaacaga tgaaattgag caagcaaata actaagatgc
    27421 gagacaattt atagtagaac aatataaacc aagattttca cattactaat tgtgagaatc
    27481 ttggtttttt tgagggttta tgaataatag aaatatatat ttatcgtaag ggattttata
    27541 gtaataactt taagttattg ttcgtaatgt gtacctgtta aataaaaata aacactttca
    27601 gcgatgttaa taatatgatc accaatacgt tctaaatgtc ttgctgctaa atgagcttgt
    27661 gcagcgacaa atggatcgtt atcaataaga tacgttgcgt taataatatg actatataag
    27721 tcatcgatat cttcatcacg ctcaattatt tctcttatta atacggtatc tttcttttta
    27781 aatgcttgat ctaagtcctt taacattaac atagctaatt tacccattgt ctttaaacgg
    27841 gttaacacat aatcatctgt aatctttgta cgcaatcgaa tattggcaat actcgaggca
    27901 ttatctccta ttctttctaa atcggaggcg atttttaatg aagaaatcat catacgcaaa
    27961 tcactcgcaa tgggctgttg ctttgtaatt aacatgataa ctcgctcatt aatatcataa
    28021 tttaattgat tgatatgttt atcgttttta actgtttgtc gtgcaaagcc tctatcgtca
    28081 atacttaatg attttatacc attttcaata ctcacataga catttgcacc taaccgacgt
    28141 aattctttta ttaaatcatc aagttgctcc tgatatcgtt gtctaattat tgccattata
    28201 tatcaaccaa accttcctga aatataatct tctgttttct tgtttgatgg gttagagaaa
    28261 attttatcag tatcatcata ttcattgaca taaccattta agaaaaatgc agttttatct
    28321 gatacacgag ctgcttgttg catattatgt gtaaccataa taattgtata cttttctttt
    28381 agttcttgaa ccaactcttc tactcttaat gttgagattg gatctaatgc tgatgtcggt
    28441 tcatccatta aaatgacttc aggttcaatt gctaaacaac gcgcgataca aacacgttgt
    28501 tgttgcccac cggataaact atatgcattt gtgtgcaacc tatcctttaa ttcatcccaa
    28561 attgcagcgc cacgtaatga tttctcaacg atttcatcaa gaactttttt atttttaata
    28621 ccgtgaatct ttggaccgta agtaatatta tcgtatattg attttggaaa tggattaggt
    28681 tgttgaaaga ccatgcccac atttgtacgt aattgttctt tagaatattt ttgatcaaaa
    28741 atgtcttgat ctcgatataa tattttacca gctgttttca cagaaggtac taactcaacc
    28801 attcgattca aagttttaat atatgttgat ttgccacaac cagatggacc tataatggca
    28861 gtaatttggt tttcataaat atctaaatta atattttgta atgcatgatt ttcgccatac
    28921 cataagtcta aattttgtgt tgaatatata acagaatgtg agtttgtatc atctggtgtt
    28981 ttatgatgac tttgtgagac atcaaacgta tgactttgag atatttgttt agtttgtgca
    29041 agtgtttgcg ccatatttaa aactcccttt ttaatagata aatgagattg attgtttaaa
    29101 ttagaatttt ttactaaatt tgttacgtaa aataatcgca acgccattca ttaagattaa
    29161 gataactagt aaaacgataa tgcctgccga tgcaacattc tggaattctt cttgaggcat
    29221 tttcgcccaa gtaaatattt ggataggtaa tgctgaaaat tgatccaata tacttctagg
    29281 tgttgccaat aatatagtcg gtataccgat tagcacaagt ggcgctgttt ctcccagtgc
    29341 tcttgaaaga gacaaaatga atccagttaa aataccaggt aacgctgctg gtaagacaac
    29401 acgtcttatc gtttgccatt tattagcacc taagccgtaa gaagcttcgc gtactgagtt
    29461 aggtacagct ctaattgctt cctgacttga aacaataata attggtaata tcagtaaggt
    29521 cattgttagc gctgctgcca atatactgtt acccattttc aaggcttcaa tccctgcacc
    29581 accaacgaac aaagtataac ctaataaccc aaatacaact gatggtacac cagctaaatt
    29641 ggaaatactg attttaacaa actgagtaaa tttgttgttt ttcgcatatt cttctaagta
    29701 tatagctgta cctattccta ggatgattga taatggaatg atacttaaca ttaaccaaag
    29761 tgaaccgatt aacgcgcctt taacgccagc catagatggt gttgaagaag aaaaattagt
    29821 gaaaaactgt aaatttaaat gacttacccc tttaatcaat gtttgagtta acaacgcaat
    29881 aagtacgaca agtcctaata atgtacatgc taaaaatatg agtttgaaca ctttattttt
    29941 aaccgttctg gatgataaat gtttttggac aagttgttga tcgacgagtg attgtctatt
    30001 attatctgtc gtttccatat taatactcct ccctaaaacg cttagaaatc cactgagaaa
    30061 gtaaattcat gattaaggta aagataaata gtgtgaaccc tacagcataa atactgtaat
    30121 aaatatttga tccaaatgtt gcatcacctg tcgctatctc aacaatatat ccagtcattg
    30181 tttgaatcga acttgttaaa cttaatgaag ctgttggcga actacctgcc gctaatgata
    30241 caatcatcgt ttctccaatt gctcttgaaa tcgcgagaac gattgaagct acaatacctg
    30301 atgttgctgc gggaagtacg actttagttg ctacttctaa tttagttgct ccaagtccat
    30361 aggcaccttc tcgaatttta tttggtacag atgccattgc atcctcactc aaacttgtga
    30421 tgagagggac aatcataata ccgacaacta agccgggact tatagcatta aactctccaa
    30481 gacctggtat gaaagatctt aatactggtg taacaaaggt taatgcaaag aaaccaaaca
    30541 caattgttgg tattcctgct aaaatttcta atatcggttt aattatgcgt cgtgcacggt
    30601 cacttgcata ttcacttaaa taaattgctg caccaagccc gactggaact gcaaatatag
    30661 tcgcaataac tgtgattttt aaagtcccta ttatcaatgc ccagatacca aacttagggt
    30721 ctgaaccggt aggattccaa gtagtagaaa atagaaattc agttattgga attctggtga
    30781 aaaaagtgat ggtttctaaa agcaatgtga ttaatatacc tagtgttgtt aaaatggaaa
    30841 tcgctgaaat tgcggctaaa ataactggta taattttgtc attatgcttt ccttttttat
    30901 tattattttt ttcgattaaa gctttaacat tagtagatga agtcataatc aaacctcttt
    30961 ctattactcc caaaataatt agatttgtaa aataatgatg aaccactatt ttaagttgat
    31021 aagcttgtag actaactgaa tgtcaaaatc agcgtctcga taaatcaagc tttgtactct
    31081 aaataatgat tcaccatcaa aatcatgttt caagacacat ttgaaattgc gtcttattat
    31141 tttttgtctt cagacttttt atcatcagat ttcttgtcgt ctgatttttg atttttatca
    31201 ataaatgctt ttaaatcatc taattgtgat ttgtatgttt tctctggtgc tgctacatat
    31261 ccagcttctt cagctgcttt acctttatct tctaagacga atttgataaa ttctgacatt
    31321 actttattat ctttcaatgc tttttcattt acataaatga ataatggtct acttaatgca
    31381 taagagttat cttgaattgt ttttttcgta ggctctgttg ctttaccatt ttcatctttg
    31441 attttaactt cttttaattt atctttattt tgtacgtaga agttatatcc aaagtatccg
    31501 attccctctt tgtttttcgt tacagaagaa acgatagcat ttgtatcagc atttttttct
    31561 gctttaatat cttctttatt cattacttca ttttcaaaga agtcataagt accatgactt
    31621 gagtttggtg atacagcatt tattttttta tctggccatt tactattaac atctttccat
    31681 gttttagctt ttccagaata aattgctttt aattgctgtt tgtctaattc atctacaaaa
    31741 tcattttctt tatttacagc aaccgttaca ccatcttgcg caattttgaa ttctttgtat
    31801 ttgatattct tatcttgtaa tttttgcttc tcttcatctt taattggtct agaagcatca
    31861 gcgaagtcga tatctcctgc aatgaatttt tggaaaccag cacctgtacc agcttgtcct
    31921 gctgagattt tagcatccga gtgatcttga gcccattttt catttaattt ctccacaatt
    31981 ggtgctactg ttgatgagcc atcaccttta gcttcccctt ttaaatcact attaccactg
    32041 ccaccattac cgccaccaca agcacctaat aatagtgttg cacctaaagc tgtagtacca
    32101 acaaattgcc attttttcat tgaaatatcc tccctgtatg aacaacaaat agtatttgta
    32161 ttgattacat ctttatctta accttgaatt attaacctca tttttagaaa ctg
//
LOCUS       DALAAR010000029        31657 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000029 DALAAR010000000
VERSION     DALAAR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.029"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            174..6989
                     /gene="sasA"
                     /locus_tag="O2458_001350"
     CDS             174..6989
                     /gene="sasA"
                     /locus_tag="O2458_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCZ3887217.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            7119..8330
                     /gene="secY2"
                     /locus_tag="O2458_001351"
     CDS             7119..8330
                     /gene="secY2"
                     /locus_tag="O2458_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCZ3887218.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8341..9894
                     /gene="asp1"
                     /locus_tag="O2458_001352"
     CDS             8341..9894
                     /gene="asp1"
                     /locus_tag="O2458_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCZ3887219.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9884..11452
                     /gene="asp2"
                     /locus_tag="O2458_001353"
     CDS             9884..11452
                     /gene="asp2"
                     /locus_tag="O2458_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCZ3887220.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11430..12389
                     /gene="asp3"
                     /locus_tag="O2458_001354"
     CDS             11430..12389
                     /gene="asp3"
                     /locus_tag="O2458_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCZ3887221.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12379..14769
                     /gene="secA2"
                     /locus_tag="O2458_001355"
     CDS             12379..14769
                     /gene="secA2"
                     /locus_tag="O2458_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCZ3887222.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14787..16295
                     /gene="gtfA"
                     /locus_tag="O2458_001356"
     CDS             14787..16295
                     /gene="gtfA"
                     /locus_tag="O2458_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCZ3887223.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16288..17646
                     /gene="gtfB"
                     /locus_tag="O2458_001357"
     CDS             16288..17646
                     /gene="gtfB"
                     /locus_tag="O2458_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCZ3887224.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17888..19795
                     /gene="sasF"
                     /locus_tag="O2458_001358"
     CDS             17888..19795
                     /gene="sasF"
                     /locus_tag="O2458_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCZ3887225.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            19963..20523
                     /locus_tag="O2458_001359"
     CDS             19963..20523
                     /locus_tag="O2458_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ3887226.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20790..22649)
                     /locus_tag="O2458_001360"
     CDS             complement(20790..22649)
                     /locus_tag="O2458_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCZ3887227.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22860..25841
                     /locus_tag="O2458_001361"
     CDS             22860..25841
                     /locus_tag="O2458_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ3887228.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(25953..26891)
                     /gene="manA"
                     /locus_tag="O2458_001362"
     CDS             complement(25953..26891)
                     /gene="manA"
                     /locus_tag="O2458_001362"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCZ3887229.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26905..28857)
                     /locus_tag="O2458_001363"
     CDS             complement(26905..28857)
                     /locus_tag="O2458_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCZ3887230.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(28934..30808)
                     /locus_tag="O2458_001364"
     CDS             complement(28934..30808)
                     /locus_tag="O2458_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ3887231.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(31048..31506)
                     /locus_tag="O2458_001365"
     CDS             complement(31048..31506)
                     /locus_tag="O2458_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887232.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT        10941 a         5024 c         5908 g         9784 t
ORIGIN      
        1 cgagaaaaag taattttaaa aatatgaaaa cgattacatt tagacgaata aaaatgaatt
       61 tttacaaatt ttaacaaaat gagtattttt gtatcaaaaa agagttgtat taaattgaat
      121 agtggcatat attataatct aatgaatgta aagataatac aaggagttat tacatgagta
      181 aaagacagaa agcatttcat gacagcttag caaacgaaaa aacaagagta agactttata
      241 aatctggaaa aaattgggta aaatccggaa ttaaagaaat agaaatgttc aaaattatgg
      301 ggctaccatt tattagtcat agtttagtga gtcaagataa tcaaagcatt agtaaaaaaa
      361 tgacgggata cggactgaaa actacggcgg ttattggtgg tgcattcacg gtaaatatgt
      421 tgcatgacca gcaagctttt gcggcttctg atgcaccatt aacttctgaa ttaaacacac
      481 aaagtgaaac agtaggtaat caaaactcaa cgacaatcga agcatcaaca tcaacagccg
      541 attccacaag tgtaacgaaa aatagtagtt cggtacaaac atcaaatagt gacacagtct
      601 caagtgaaaa gtctgaaaag gtcacttcga caactaatag tacaagcaat caacaagaga
      661 aattgacatc tacatcagaa tcaacatcct caaagaatac tacatcaagt tctgatacta
      721 aatctgtagc ttcaacttca agtacagaac aaccaattaa tacatcaaca aatcaaagta
      781 ctgcatcaaa taacacttca caaagcacaa cgccatcttc ggtcaactta aacaaaacta
      841 gcacaacgtc aactagcacc gcaccagtaa aacttcgaac tttcagtcgc ttagctatgt
      901 caacatttgc gtcagcagcg acgacaaccg cagtaactgc taatacaatt acagttaata
      961 aagataactt aaaacaatat atgacaacgt caggtaatgc tacctatgat caaagtaccg
     1021 gtattgtgac gttaacacag gatgcataca gccaaaaagg tgctattaca ttaggaacac
     1081 gtattgactc taataagagt tttcattttt ctggaaaagt aaatttaggt aacaaatatg
     1141 aagggcatgg aaatggtgga gatggtatcg gttttgcctt ttcaccaggt gtattaggtg
     1201 aaacagggtt aaacggtgcc gcagtaggta ttggtggctt aagtaacgca tttggcttca
     1261 aattggatac gtatcacaat acatctaaac caaattcagc tgcaaaggcg aatgctgacc
     1321 catctaatgt agctggtgga ggtgcgtttg gtgcatttgt aacaacagat agttatggtg
     1381 ttgcgacaac gtatacatca agttcaacag ctgataatgc tgcgaagtta aatgttcaac
     1441 ctacaaataa cacgttccaa gattttgata ttaactataa tggtgataca aaggttatga
     1501 ctgtcaaata tgcaggtcaa acatggacac gtaatatttc agattggatt gcgaaaagtg
     1561 gtacgaccaa cttttcatta tcaatgacag cctcaacagg tggcgcgaca aatttacaac
     1621 aagtacaatt tggaacattc gaatatacag agtctgctgt tacacaagtg agatacgttg
     1681 atgtaacaac aggtaaagat attattccac caaaaacata ttcaggaaat gttgatcaag
     1741 tcgtgacaat cgataatcag caatctgcat tgactgctaa aggatataac tacacgtccg
     1801 tcgatagttc atatgcgtca acttataatg atacaaataa aactgtaaaa atgacgaatg
     1861 ctggacaatc agtgacatat tattttactg atgtaaaagc accaactgta actgtaggca
     1921 atcaaaccat agaagtgggt aaaacaatga atcctattgt attgactaca acggataatg
     1981 gtactgggac tgtgacaaat acagttacag gattaccaag cggattaagt tacgatagtg
     2041 caacgaattc aatcattggg acaccaacaa aaattggtca atcaacagtg acagttgtgt
     2101 ctactgacca agcaaataac aaatcgacga caacttttac aataaatgtt gtggatacga
     2161 cagcaccaac agtgacacca ataggagatc aatcatcaga agtgtattca ccaatatccc
     2221 cgattaaaat tgctacgcaa gataacagtg gaaatgcggt gacgaataca gtgactggat
     2281 tgccatccgg actaacattt gatagtacaa ataatactat tagtggtaca ccaacaaaca
     2341 ttggtacaag tactatatca atcgtttcta cagatgcgag cggtaacaaa acgacgacaa
     2401 cttttaaata tgaagtaaca agaaatagca tgagtgattc cgtatcaaca tcaggaagta
     2461 cacaacaatc tcaaagtgtg tcaacaagta aagctgactc acaaagtgca tcaacgagta
     2521 catcaggatc gattgtggta tctacatcag ctagtacctc gaaatcgaca agtgtaagcc
     2581 tatctgattc tgtgagtgca tctaagtcat taagcacatc tgaaagtaat agtgtatcaa
     2641 gctcaacaag cacaagttta gtgaattcac aaagtgtatc atcaagcatg tcggattcag
     2701 ctagtaaatc aacatcatta agcgattcta tttcaaactc tagcagtact gaaaaatccg
     2761 aaagtctatc aacaagtaca tctgattcat tgcgtacatc aacatcactc agtgactcat
     2821 taagtatgag tacatcagga agcttgtcta agtcacaaag cttatcaacg agtatatcag
     2881 ggtcgtctag tacatcagca tcattaagtg acagtacatc gaatgcaatt agtacatcaa
     2941 catcattgag cgagtcagct agcacctcgg actctatcag tatttcaaat agcatagcca
     3001 actctcaaag tgcgtcaaca agcaaatcag attcacaaag tacatcaata tcattaagta
     3061 caagtgattc aaaatcgatg agtacatcag aatcattgag cgattcgacg agcacaagtg
     3121 gttctgtttc tggatcacta agcatagcag catcacaaag tgtctcaaca agtacatcag
     3181 actcgatgag tacttcagag atagtaagtg actctatcag tacaagtggg tcattatctg
     3241 catcagacag taaatcaatg tccgtaagta gttcaatgag cacgtctcag tcaggtagta
     3301 catcagaatc attaagtgat tcacaaagta catctgattc tgatagtaag tcattatcac
     3361 aaagtactag tcaatcaggt tcaacaagta catcaacgtc gacaagtgct tcagtacgta
     3421 cttcggaatc acaaagtacg tctggttcaa tgagtgcaag tcaatccgat tcaatgagca
     3481 tatcaacgtc gtttagtgat tcaacgagtg atagcaaatc agcatcaact gcatcaagtg
     3541 aatcaatatc acaaagtgct tttacgagca catctggttc ggtaagtact tcgacatcgt
     3601 taagtacaag taattcagaa cgtacatcaa catctatgag tgattccaca agcttaagta
     3661 catcagagtc tgattcaata agtgaatcaa cgtcaacgag cgactctata agtgaagcaa
     3721 tatctgcttc agagagcacg tttatatcat taagtgaatc aaatagtact agcgattcag
     3781 aatcacaaag tgcatctgcc tttttaagtg aatcattaag tgaaagtacg tctgaatcaa
     3841 catcagagtc agtgagtagt tcgacaagtg agagtacgtc attatcagac agtacatcag
     3901 aatctggtag cacatcaaca tcattaagta attcaacaag tggtagtacg tccatttcaa
     3961 catcgacaag tatcagtgaa tcaacgtcaa cgtttaagag cgagagtgtt tcaacatcac
     4021 tgagtatgtc aacgagtaca agtttgtctg actctacaag tttgtcaaca tcattaagtg
     4081 attccacaag tgatagtaag tctgattcat taagtacatc aatgtcgaca agtgattcaa
     4141 tcagtacaag taaatctgat tccattagta catccacatc attaagtggt tctacaagtg
     4201 aaagtgaatc cgactcaaca tcatcaagtg aaagtaaatc cgattcaaca tcaatgagca
     4261 taagtatgtc tcaatcaaca tcaggaagta caagtacgtc aacgagtaca agtttgtctg
     4321 actcaacgag tacatcattg tcactaagtg cctcaatgaa tcaaagcgga gtagactcaa
     4381 actcagcaag ccaaagtgcc tcaaactcaa caagtacaag cacgagcgaa tccgattcac
     4441 aaagcacatc atcatataca agtcagtcaa caagccaaag tgaatccaca tcgacatcaa
     4501 cgtcactaag cgattcaaca agtatatcta aaagtacgag tcaatcaggt tcggtaagca
     4561 catcagcgtc attaagtggt tcagagagtg aatctgattc acaaagtatc tcaacaagtg
     4621 caagtgagtc aacatcagaa agtgcgtcaa catcactcag tgactcaaca agtacaagta
     4681 actcaggatc agcaagtacg tcaacatcgc tcagtaactc agcaagcgca agtgaatccg
     4741 atttgtcgtc aacatcttta agtgattcaa catctgcgtc aatgcaaagc agtgaatccg
     4801 attcacaaag cacatcagca tcattaagtg attcgctaag tacatcaact tcaaaccgca
     4861 tgtcgaccat tgcaagttta tctacatcgg taagtacatc agagtctggc tcaacatcag
     4921 aaagtacaag tgaatccgat tcaacatcaa catcattaag cgattcacaa agcacatcaa
     4981 gaagtacaag tgcatcagga tcagcaagta catcaacatc aacaagtgac tctcgtagta
     5041 catcagcttc aactagtact tcgatgcgta caagtactag tgattcacaa agtatgtcgc
     5101 tttcgacaag tacatcaaca agtatgagtg attcaacgtc attatctgat agtgttagtg
     5161 attcaacatc agactcaaca agtgcgagta catctggttc gatgagtgtg tctatatcgt
     5221 taagtgattc gacaagtaca tcaacatcgg ctagtgaagt aatgagcgca agcatatctg
     5281 attcacaaag tatgtcagaa tctgtaaatg attcagaaag tgtaagtgaa tctaattctg
     5341 aaagtgactc taaatcgatg agtggctcaa caagtgtcag tgattctggc tcattgagcg
     5401 tctcaacgtc attaagaaaa tcagaaagtg taagcgagtc aagttcattg agttgctcac
     5461 aatcgatgag cgattcagta agcacaagcg attcgtcatc attaagtgta tcgacgtcac
     5521 taagaagttc agaaagcgtg agtgaatctg attcattaag tgattcaaaa tcaacaagtg
     5581 gttcgacttc aacaagtaca tctggttcat tgagtacctc aacatcatta agtggttcag
     5641 aaagcgtaag cgagtctacc tcgctaagtg attcaatatc aatgagtgat tctactagta
     5701 caagtgactc cgactcatta agtggatcaa tatctttaag tggttccaca agtcttagca
     5761 cttcggattc attaagtgat tcaaaatcat tgagtagctc gcaaagtatg agtggatcag
     5821 aatcaacgtc aacaagtgtg agcgattcgc agtcaagctc aacaagtaat agtcaatttg
     5881 actctatgag catcagtgca tcagaaagcg actcaatgtc tacaagtgat tcgtctagca
     5941 tcagtggatc aaattcaacg agtacatcac tttcaacatc tgactcaatg agcggaagcg
     6001 tatcagtttc aacatcgaca agtttaagtg actcaatatc aggttcaaca agtgtaagtg
     6061 actcgagctc aacaagcaca tctacatcat taagtgattc aatgtcacaa agccagtcaa
     6121 caagtacaag tgcatctggt tccttaagta catcgatatc aacatcaatg tcaatgagtg
     6181 ctagtacatc gtcatcacaa agcacatcgg tgtcgacatc attatcaaca tcagacagta
     6241 tcagtgattc tacttcaata agtatcagtg gttcacaaag tacagtagaa tcagaatcta
     6301 caagtgattc aacttctatc agtgactcag aatcattgag tacatcagat tcagactcga
     6361 catcgacaag tacatcggac tcaacaagtg gttcaacttc aacaagcata tctgaatcat
     6421 taagtacgtc tggttcaggt tcaacgagcg tatctgactc aacatcaatg agtgaatcta
     6481 attcatcgag tgtttcaatg tcacaagaca aatccgactc aacatcaatt agtgactcag
     6541 aatcagtgtc aacaagcaca tcaacgtcat tgagcacatc cgattcgaca agcacatccg
     6601 aatcactgag tacatctatg tctggttcac aaagcatttc tgactcaaca tcaacaagta
     6661 tgtccggctc aacaagtaca tccgaatcta actcaatgca tccgtcagac tcaatgagta
     6721 tgcatcatac tcacagcacg agcacatctc gcttatcaag tgaagcaaca acgagcacga
     6781 gtgaatctca gtctacatta agtgcaacat ctgaagtgac taaacataat ggcacaccag
     6841 cacaaagtga aaaaagattg ccagatacag gtgactcaat aaaacaaaat ggattactag
     6901 gtggcgttat gacattatta gttggtttag gtttaatgaa gagaaagaaa aagaaagatg
     6961 aaaatgatca agatgattct caagcataaa ttgaatttat agctggctaa aattaaaaag
     7021 actgaggcac caatcactag aaaaatcgat ataggatttt aataattagt gatgaaccct
     7081 tagtcttttt aatgtttttg atataaaaga ggtgcaacat gttaaaactt ttacaacaat
     7141 acgaatataa aattatatat aaacgtatgc tatacacatg ttttatcttg tttatttata
     7201 tattaggcac aaacatttcc atcgtttcct ataacgatat gcaagtaaaa catgagtcat
     7261 tttttaaaat agctatttct aatatgggtg gagacgttaa tactttaaac attttcactt
     7321 taggattagg tccgtggtta acatcaatga ttatcttgat gctgatttct tatcggaata
     7381 tggataaata catgaaacaa acaagtcttg agaagcatta taaagagcgc atcttaacat
     7441 taattttaag tgttattcaa agttattttg tgattcatga atatgtttcg aaagaacgtg
     7501 ttcatcaaga caatatttac ttaacaatat tgattttggt tactggaacg atgttattag
     7561 tttggttagc tgacaaaaat agtcgctacg gcatcgcagg accaatgccg attgtaatgg
     7621 ttagtattat taaatcaatg atgcatcaaa aaatggaata tatagatgca agtcatattg
     7681 tgattgcgtt gttaatcatc cttgtgataa tcacattgtt tatcttatta tttattgaat
     7741 tagtagaagt gagaatacca tatatcgatt taatgaacgt ttccgcaaca aatatgaaat
     7801 cttatttatc ttggaaagtt aaccctgcag gcagtattac tttaatgatg agtatttcag
     7861 cttttgtttt cttaaaaagt ggcattcatt ttattttatc tatgtttaat aaaagcatat
     7921 cagatgacat gccaatgctg acatttgata gtccagtagg tatttcagta tatttagtga
     7981 ttcaaatgtt attgggttat tttttatcga gatttttaat caatactaaa caaaaatcca
     8041 aagatttctt aaagagtggc aattattttt caggagttaa acctggtaag gatacagaac
     8101 gttatttaaa ttatcaagca cgacgcgtat gttggtttgg attggcatta gttacagtca
     8161 ttattggtat accgctttat ttcacattgt ttgtaccgca tttatctact gaaatttatt
     8221 tctcagtaca actgattgta ttagtttaca tcagtattaa tattgcagaa acaattcgta
     8281 catatttata ttttgataaa tataagccat ttttaaacca gtattggtaa ggaggtaatt
     8341 atgaaatact ttattccagc ttggtacgat gaccaacgat ggtggcaaga cacgactgtg
     8401 ccgtattatc aactacaaaa taagacggaa tttgacgata tgattagttt aatgggaatg
     8461 caccttgaaa atgacttaga ttatcaactg attgttctca atcatgcacc aaatttaaga
     8521 acatttttac atcgatatga cttatatgag acaaagtatt cgtctgtgtt tgatgaaatt
     8581 caaggattca gtcaccatgc gccacaagcg attaattatc atcacttaaa atggccggat
     8641 gatgttgagt ttgtgtacac accgtattta ttaaaatgtg tgacgagtga acagacctat
     8701 acaaatattt attttagtca agaagggtat tcaatttggt ttgaagaatt tgaaagagat
     8761 cagttacaac ggcgttatat ttttgatgac agaggttatt tatcagcgat acgttatttt
     8821 gatgatcagg gagaggcttc ttaccaagaa tatttaacga ttaatggaga ttgtgtactt
     8881 catgaagatt tgaaaaatgg cagagtcact gtttcaaaaa gatatcaaca tcactatcaa
     8941 caaacagaat ataacaatat ggctcaacta attgaagaaa aatttcaagc aatgattgca
     9001 caacaaatac atgaagatga tcatgtgatt gtggcttcag atgctaggca caatcgacaa
     9061 atagccaatc atattccagc gaaatcatta agttattcat tttttaaaaa tagaaatgaa
     9121 actgtgtcag atgaggaata tcaatctatc gtaaagaatg cccatttaat tgttgatagt
     9181 gtgcaactag aacgtgattt aattagtcat caagagaagt atcagcggga gaatacaatg
     9241 attcgaatca caccatttga aacgagacaa tcacctaata taagtagtca attgatggaa
     9301 acatttatag gtgtatggat agatggtatg agtgacgctg atttgcaaca aatgatgcaa
     9361 cgacttgtgg attatatcgc acaggaagat tattaccgtt taattttatt atcgcgccat
     9421 caaaatgaca taccgatgtg gcttcgtgaa tgtattacgt cggtaaatga ggaataccaa
     9481 gctaaacaga atgcggatgt taatgtttca gcattaatga cacctgaaga tcaagatgac
     9541 atcattgctg ttaagacgat acatgctgaa catgatgttg tagaagcatt gcggacgttg
     9601 cgacttgtga tagatatgtc aaaagaacct gacttgtatt tacaaattag tgcaattagc
     9661 gctgggattc cacaaattaa tggtcaacaa acagattacg tctctgatta tgacaatggc
     9721 cgtattataa atacagttga tgaattagat gatgcgttaa attattattt attttatttg
     9781 aaaaattgga attatgcgta cgcctattct ttaaaattaa tagatgcata tgcttctaag
     9841 aatattatta atcagctcga tgagttaata gaaggtgaaa atgatgccac gtaaatttag
     9901 agttttgcaa attggaggag acgatttaga acctattttt caacacaaaa aaggtgtgag
     9961 ttgggattac ttcgatattg gattgtttga atttgatagt ggttatgtag aggctattga
    10021 agcgattgtt gaagcagaag ggcgctttga ttttatctat attcaagcac catactcgga
    10081 gacattaacg aatttattac aaatgataag cgaaccatac aatacgtatg ttgatgaatc
    10141 attttggtca gttgaatatg aacaagacga aaatgtccaa aaatacgttg ttcaaccatt
    10201 acattaccgg aatattgaag aacgtaataa taaattagag gcagttagct tctcaggaca
    10261 gtatggagat aaggtttctc ctaagttagc acttgtgcat ccgaatttta aaggagatgt
    10321 cgtttaccaa ggtaattcag agctcacatt gagtggagaa tttgggaaag aatttaaacc
    10381 tatcgcatct tggcaaaata atctcgttta cgataaagat aaagtcattc aaatatggcc
    10441 agaatttgat attgatggtg cggtcgagtt gcaatataca tttagattga ttcagactgg
    10501 cgctgatggt gcattaattg aacaaatcgt attgactgat gatatgttag acagtccttt
    10561 agagatacct gcgaaaccat ttgatgctta tataagtgta actgttaagg cgcgtgggaa
    10621 cgggacggta catttaggac ctatacacaa acgatggtct agattagaca tgggtcaatt
    10681 tttacttggt ggtagtcgtt tcgtagatag tcaacgacag gaatttattt attatttcca
    10741 ccctggtgat atgaaaccgc cactaaacgt atattttagt ggttatcgaa cagcggaagg
    10801 tttcgaagga tattatatga tgaaacgtat gaatgcaccg tttttactta tcggcgatcc
    10861 tcgtgttgaa ggtggtagtt tttatatcgg ttcatctgaa tatgaacaag gtattatcaa
    10921 tgttattgac gagacattag aaaagctgaa ttttaaatca catgaattaa tattatctgg
    10981 tttatctatg ggttcatttg gtgccttgta ttatggtgct cagttaaatc ctcaggcaat
    11041 tattgttggt aaaccacttg ttaatattgg aacgattgct gaacatatgc gattacttag
    11101 accagaagag ttcggtacag cattagacgt cctggtgtct aatgaaggtg atacatctca
    11161 agcgtcgata caggcattaa atcaaaaatt ttggcaaaca tttcaaaaga aatcattgtc
    11221 acaaactgtt tttgccatag catatatgca acatgatgat tatgatccac atgcatttca
    11281 agagctatta cctgtattga cagcacacca agcaagagtg atgaatcgca gtattcctgg
    11341 gcgacataat gatgattcac ctaccattgc aagttggttt gttaattttt acaacattat
    11401 tttggaagac aagtttggga gagttcaaca tgctgaaaaa caaaacattt aaagttactt
    11461 ggaattatat ttcacaaact acatttatgt atggctctaa agtatcattt tcaaatggtg
    11521 aagtgacttt tataaatcct ttgatgccct ctggattacc gattcatgag tggttgatgt
    11581 taaagcaatt ttcaaaatat aaaagtgcgc caagtttacc aattttgcgt agaggacagc
    11641 attataaatt gcattttgat tttgatgcaa cgcctgctgg ttcagtgtat tttatcatca
    11701 ttttttataa taaaaatggc acaaaactaa gtactgagat tgtaaagtca aatagtatta
    11761 cgattcaata tcctgatgaa gcctatgctt ataaaataaa aatgatgaat gcagcatcga
    11821 cgagtcttat atttagatgt ttaacaatca cagagatgac gcatcaatat gatttagagt
    11881 ataaaagcat gcgggtgacg aagataggcg atgaccaata tggaaatgat atgatcaatg
    11941 tcattattgc agaaccatct gacacctatc caactatttc gaatgatttt ttgaaactgt
    12001 ttggtcatgt ttggctcgtt gaacgttgga tggacgacaa tatcgaggga aatattaagc
    12061 aactaaagaa tgatttgcaa agtcaagata ccctaaaggc tattaatttg attagttatg
    12121 ggtcaaagtc aaatgttttt gcaacttatg tcgctcagca tttgggttgc aaagtgtatc
    12181 gtacaagcca tgaagatgat gatttaaaag gatggcttac agaacatgtc ccaggaaatc
    12241 atgaactaaa agatacaaat gtcgaggttt attttaaaga agagcaagac aaacatctaa
    12301 attatatgtc acgtttaatg aatccagtac gatttttaga ttacttagat gtatcgaaac
    12361 tgaatggtgg ggaagtaaat gaaacataaa ctagatgtta cgataaacga attaagacta
    12421 aagtcgatta gaaaaatagt gaaacgcatc aatacatgga gcgatgaagt taaatcatat
    12481 tctgatgatg cgttaaaaca aaagacaata gaatttaaag aacgtttagc atcaggagtt
    12541 gatacactag atacattgtt acctgaagct tatgcagtgg cacgtgaagc gagctggaga
    12601 gtattaggta tgtatcctaa agaagttcag ttaatcggag cgattgtatt gcatgaaggt
    12661 aacattgcgg agatgcaaac aggcgaaggt aaaacattaa cggcaacgat gcctttatat
    12721 ttaaatgctc tttcgggaaa aggaacatat ttaattacaa cgaatgatta tttagcgaag
    12781 cgagattttg aagagatgca accattatat gaatggttag gtttaactgc ttcattaggt
    12841 tttgttgata ttgtggatta tgagtatcaa aaaggggaaa aacgtaatat atatgaacat
    12901 gatattatat atacaactaa tggacgtctc ggttttgatt atttaattga taatttggct
    12961 gatagtgcgg aagggaaatt tttaccacaa ttaaattacg gtattattga tgaagtggat
    13021 tcaatcattt tagatgctgc tcaaacacca ttagttattt cgggtgcacc aagattacaa
    13081 tcaaatctat ttcatattgt gaaagagttt gtagatacat tgattgaaga cgtgcatttt
    13141 aaaatgaaga agaccaaaaa agaaatatgg ctgttaaatc aaggtattga agcggcacaa
    13201 tcatacttta atgttgaaga tttatatagc gaacaagcga tggtcctagt gcgtaatatt
    13261 aatttagcac tgcgcgcaca atatttgttt gaatctaatg tcgattactt tgtatataat
    13321 ggtgatattg ttttaattga ccgtattaca ggtcgtatgt taccgggaac taagttgcaa
    13381 gctggacttc accaagctat tgaagcgaaa gaaggtatgg aggtttcaac agataaaagt
    13441 gttatggcaa caattacctt ccagaattta tttaaacttt ttgaatcatt ttcaggtatg
    13501 acagctacag gaaaattagg cgaatcagag ttctttgatt tgtattcaaa aatagtcgta
    13561 caagtaccaa ctgataaagc gattcaacgt atcgatgaac cagataaagt gtttcgttca
    13621 gttgatgaga aaaacatcgc gatgattcat gatatagttg aacttcatga aacggggcga
    13681 ccggttttac tcataacgag aactgctgaa gcggctgaat acttttcgaa agtattattc
    13741 caaatggata ttcctaataa tttactcatt gcgcaaaatg ttgcaaaaga agcgcagatg
    13801 atagctgaag cagggcaaat tggttccatg actgttgcga ctagtatggc aggtcgaggc
    13861 acagatatta aacttggtga aggtgtcgaa gcattaggtg gattagctgt tattattcat
    13921 gaacatatgg aaaatagccg tgtagacagg caattacgtg gtcgttctgg tagacaaggg
    13981 gatccgggat catcttgtat atatatttca ctagatgatt atttagttaa gcgatggagc
    14041 gatagtaatt tagcggaaaa taatcaatta tattcattag atgcacaacg attatcgcaa
    14101 agtaatttgt ttaatcgcaa agttaagcaa attgtagtta aagcgcagcg tatctcggaa
    14161 gaacaagggg ttaaagctcg tgaaatggct aatgaatttg aaaaaagcat tagtatacag
    14221 cgagatcttg tatacgagga acgcaatcga gttttagaaa tagatgatgc tgagaatcaa
    14281 gattttaaag cgttagctaa agatgtattt gaaatgtttg taaatgagga aaaggtgcta
    14341 acaaaatcgc gtgttgtcga gtatatttat caaaatttaa gtttccaatt taataaagat
    14401 gtggcttgtg ttaattttaa agataagcaa gcagtagtga catttttatt agagcaattt
    14461 gaaaagcaat tagctttgaa tcgtaaaaac atgcaaagtg catattatta taatattttc
    14521 gttcaaaaag tctttttgaa agcaattgat tcatgttggt tagaacaagt cgactattta
    14581 caacaattaa aagcaagcgt taatcaacga caaaatgggc aacgtaacgc tatttttgaa
    14641 taccatagag ttgccttaga ttcatttgaa gttatgacac gaaatatcaa aaaaagaatg
    14701 gtgaaaaata tttgtcaaag catgattaca tttgataaag agggtatgcc agttatacat
    14761 tttccataaa tgaaaagggg tagcgcatga caatatataa tataaacttt ggtataggct
    14821 gggcgagtag cggtgttgaa tatgcacaag cttaccgtgc taaattattg agacgtacaa
    14881 atcaagacac taaatttgtg tttttagatt ttatacagtc agagaacata cagacattaa
    14941 cgcaaaacat tgggtttaaa gatcaagaag ttatttggtt gtatcagtat tttagtgata
    15001 tttctttagc accaacaacg tacacgttag atgacattga acgtgagctt ggtcatgaca
    15061 ttagttttag agaaagaaat ggtaagatag tccgcctata ttttaatggg aaatcatcat
    15121 ttgtaacatg ttatctgcaa aatgaacaaa aagatattgt ggatcgtgca gaatttgtaa
    15181 ttaatagtat gttagtacgt aaagattttt atagttatac gcgcatattt tctgagtatt
    15241 atgcaccagc tgataataaa gcgaaattat atatgcgaca attttataat caagatggtt
    15301 ccatcgctta taacgaatat attgatggag acagctctat atatgtgttt gaagatgcgg
    15361 tgttgtataa caaaacggaa tttattgcat actttttaca acgattgaat ttaacccgag
    15421 acgatattgt catattagac cgcgcttctg atattggtca ggctgttttg cagcataaag
    15481 gtgatagtaa agttggtgtt gtgattcatg ctgatcatta tagtaataat atgatgagtg
    15541 agcagcatat actatggaac aattattatg aatatcaatt ttcaaaagct aaatacattg
    15601 atttctttat tacagcaaca gatattcaaa atcatatggt ttgtcgacaa tttgagcaat
    15661 atcaaggtta tcgcccacgt gtttatacta ttcctgtggg aagcattgat gcattatcat
    15721 atccaacatt atcaagaaag ccatatgcga tgattagtgc atctcgttta gcgaatgaga
    15781 aacatattga ttggcttgtc aaagcggtta tcgtggctaa acgacaagtg ccagaattaa
    15841 catttgatat ttatggagaa ggtagcgaga agacgcgact gcgtaaaatt attgatacac
    15901 accgcgcaca agattatatt cgtttactag ggcatgttaa acttgatgaa atttataatg
    15961 attatgagtt atttctttct gcatcaacta gcgaaggttt tgggttaaca ctaatggaag
    16021 cggtaggctc aggactaggt atgattggat taaatgtgaa ttatggtaac ccaaccttta
    16081 ttcgagatgg ggaaaatggc tacttggtac cttttgatac cgacgaagat agtgtggatg
    16141 atgttatcgc aaaactagct catgccattg taatgtattt taacaatggt ccccagacac
    16201 cacatgacat ctcttatgaa gtggcacagc aatttatgac tcaagatatt attttaaaat
    16261 gggaaacatt agttcaagag gtgctacatg attaaattat ttgattactt taacgatcat
    16321 tcacgaaagt tatatgagtc ttttaaagcc agtaagttgg aagaagattt aacaattgta
    16381 ctcaatgaca atggcttttt accggacgat gttatatcac cgtatcaatt ttttgcagat
    16441 aatcataata cagaaaatat gaaaccacga ttttttaatc aggttactgt accagctttt
    16501 tgggaaatta aaggtaacaa caattcggct acaattaatg atatgggacg tctacgaggt
    16561 aaaatatttt atcaaagtgg agagcgccct agaattgtta gtcgtgtcga atggtttgat
    16621 gaccaacaac gtgtccgatt tgtcgattat tattctaaga atggtattaa atttgcacaa
    16681 actgtttacg atttaaatcg taaagcgatc ttgaagaaat atatgacagc agaaggtaaa
    16741 gaagtgattt atgaaaattt tgtaacatct gatgtcatat tggattggca agggaagtct
    16801 tactttttcc cttcaaagct cgcattcgca ttatttttta tcaagcaact tgaaattacg
    16861 gaacatcatt tcgttattaa ctcactagcc ctaccatttt cagtgttata taatttgcca
    16921 tcaaaaggta gtgatgtttt ggtatggcaa gaacactgta atggtaatgt cccgggaaat
    16981 atgcaattga tgtgtaaagg tgatatgaaa cgacattgta atattattat tccagataaa
    17041 aacgaatatg aaacgatgct caatatagca gacgcaaagg ttcaatctcg tattttacaa
    17101 ggcggatatc tatataacta tcgtagtcgt aatagatata ctaaagaaat tgtaatatta
    17161 actaactcgg atcaacttcg taacatcaag gtattggttg aaactttacc agattttaag
    17221 tttcacatcg ctgctattac agagatgtct gataagctaa tgcaacttga tcaatatgct
    17281 aatgtgcatt tatacccatc tattaatatt gacagggtaa atgaactata ccagttatgt
    17341 gatatttatc ttgatatcaa tgagggtaat gaaattttaa atgcggtgga acaagcgttt
    17401 gactatgagt tgttgatttt aggttatcgt caaaccgcac accatgctaa agtaacatta
    17461 tcagagcact tatttgagca taatgatgag ataacaatgg aaagtaaaga ccaattaata
    17521 caaatgttag aaagtttgaa agatcaacaa cagtttcgag atgctttatt agcacaaaaa
    17581 gcacatgcac acgaaatctc gcgtgaacaa ttcgagcaag tttttaaaca ggcgctcgaa
    17641 agctaaaagg aaaaagcagt ctaaaataaa acatcattta atataaaaga tttgacgtaa
    17701 tatgatgtta gcgacatgga caatatgata ttacgtcatg cttgttttgt ttacttaaat
    17761 ataagctaag taataagtag ataattacta acaacaataa ctagatagat aagtgtaaat
    17821 ttcttgtaaa caggtatata atagtatgta attctttgaa ggaagtaagt gaggaaggag
    17881 tatgttgatg gctaaatatc gagggaaacc gtttcaatta tatgtaaagt tatcgtgttc
    17941 gacaatgatg gcgacaagta tcattttaac gaatatcttg ccgtacgatg cccaagctgc
    18001 atctgaaaag gatactgaaa ttacaaaaga gatattatct aagcaagatt tattagacaa
    18061 agttgacaag gcaattcgtc aaattgagca attaaaacag ttatcggctt catctaaaga
    18121 acattataaa gcacaactaa atgaagcgaa aacagcatcg caaatagatg aaatcataaa
    18181 acgagctaat gagttggata gcaaagacaa taaaagttct cacactgaaa tgaacggtca
    18241 aagtgatata gacagtaaat tagatcaatt gcttaaagat ttaaatgagg tttcttcaaa
    18301 tgttgatagg ggtcaacaaa gtggcgagga cgatcttaat gcaatgaaaa atgatatgtc
    18361 acaaacggct acaacaaaac atggagaaaa agatgataaa aatgatgaag caatggtaaa
    18421 taaggcgtta gaagacctag accatttgaa tcagcaaata cacaaatcga aagatgcatc
    18481 gaaagataca tcggaagatc cagcagtgtc tacaacagat aataatcatg aagtagctaa
    18541 aacgccaaat aatgatggtt ctggacatgt tgtgttaaat aaattccttt caaatgaaga
    18601 gaatcaaagc catagtaatc gactcactga taaattacaa ggaagcgata aaattaatca
    18661 tgctatgatt gaaaaattag ctaaaagtaa tgcctcaacg caacattaca catatcataa
    18721 actgaatacg ttacaatctt tagatcaacg tattgcaaat acgcaacttc ctaaaaatca
    18781 aaaatcagac ttaatgagcg aagtaaataa gacgaaagag cgtataaaaa gtcaacgaaa
    18841 tattattttg gaagaacttg cacgtactga tgataaaaag tatgctacac aaagcatttt
    18901 agaaagtata tttaataaag acgaggcagt taaaattcta aaagatatac gtgttgatgg
    18961 taaaacagat caacaaattg cagatcaaat tactcgtcat attgatcaat tatctctgac
    19021 aacgagtgat gatttattaa cgtcattgat tgatcaatca caagataagt cgctattgat
    19081 ttctcaaatt ttacaaacga aattaggaaa agctgaagca gataaattgg ctaaagattg
    19141 gacgaataaa ggattatcaa atcgccaaat cgttgaccaa ttgaagaaac attttgcatc
    19201 aactggcgac acgtcttcag atgatatatt aaaagcaatt ttgaataatg ccaaagataa
    19261 aaaacaagca attgaaacga ttttagcaac acgtatagaa agacaaaagg caaaattact
    19321 ggcagattta attactaaaa tagaaacaga tcaaaataaa atttttaatt tagttaaatc
    19381 ggcattgaat ggtaaagcgg atgatttatt gaatttacaa aagagactca atcaaacgaa
    19441 aaaagatata gattatattt tatcaccaat agtaaatcgt ccaagtttac tagatcgatt
    19501 gaataaaaat gggaaaacga cagatttaaa taagttagca aatttaatga atcaaggatc
    19561 agatttatta gacagtattc cagatatacc cacaccaaag ccagaaaaga cgttaacact
    19621 tggtaaaggt aatggattgt taagtggatt attaaatgct gatggtaatg tatctttgcc
    19681 taaagcgggg gaaacgataa aagaacattg gttgccgata tctgtaattg ttggtgcaat
    19741 gggtgtacta atgatttggt tatcacgacg caataagttg aaaaataaag cataattata
    19801 ttgggggaag agcatctata tattttttta agtatataag acgtcttatt tccccttaat
    19861 ttattgtgaa gtatatgcaa aatgcaatga atagattgtc catcatttta acgttataat
    19921 gaatttaacg acttagaact acacaagtaa aggagaatga agatgtctcg aaaaacggcg
    19981 ctattagttt tggatatgca agaaggtata gcgagtagtg tacctagaat aaaaaatatt
    20041 attaaagcga atcagagagc aattgaagca gcaagacaac atcgaatacc agtcattttc
    20101 atacgtttag tgttagataa gcattttaat gatgtctcct cgagtaataa agtgttttca
    20161 acaattaaag ctcaaggata tgcgattact gaagcagatg catctacacg aatacttgaa
    20221 gatttagcac cactagaaga tgagccgatt atttctaagc gacgctttag cgcatttaca
    20281 ggtagttact tggaagttta tttacgtgca aatgatatta atcatttagt attaacgggt
    20341 gtctctacaa gtggagctgt attgagcacg gcattagaaa gtgtagataa agactattat
    20401 attactgttt tagaagatgc tgttggtgat agatcagatg ataaacatga ctttattatt
    20461 gaacaaattt tatcacgctc atgtgacatt gaatccgtag agtcatggaa aagtagttta
    20521 tagttaatat aacgtcaatt aaagctcggc agtaatgttt gagaataagt acatttgctc
    20581 atatttataa aatgtgtgag atggcaattg aaacggatat gatgaggaac atttgaacat
    20641 aaaataatat atttatataa aacgacccga ggcgttcgaa ctgaatgcct cgggtttaat
    20701 tgaataagaa atcagactta tgaacagaaa tatgtttaag tccgaactcc ttgtttatac
    20761 ttataaattt tacgggttta atataatact tatttacctg taatatatga taattcttca
    20821 gcggcagctg cgttgatagt tctatgagaa atgataccta atcctttaac attggattct
    20881 gaaataacga tagaaccatc actgttaact ttttcaacaa atgctacatg accgtaatgt
    20941 tgatctgcac caaattgtcc agcctcaaat acaacagcag catgacgttt tggtgtatga
    21001 cttacttgat aatcacggta ttgagctcga ttattccaat tatgtgcatc acctaaatca
    21061 cctgagatag atgtaccaaa ttgtttcata cggttatata cgtaccaagt acattggcca
    21121 tgtggatatg gcatactatc agatacctca cggaaaggtt tgaattcatc tgatgaatca
    21181 tcataatcct tgatagaacg ttcatattta tctaaatctg gcatgcgttc atcgtcaaac
    21241 tgagttaatt gatagtgttt aataatactg tttaatttct tagcatagtt tggatctgta
    21301 gcatatgttt tagataagtg tgatgttgca tctttataag aatcggcttc cgatttccat
    21361 gttggtttat aaattgttcg attgccatca ataccatttt taataaggtc agagtaatct
    21421 tttagtgatt ctttcgtgct tggatatttt cggaatccag cattaatact atacaattga
    21481 ttaccatcag cttctaatgt gttaaaagga acagaattcc cttcaaaagc acctttgata
    21541 ccgaataaat tatggtttgg tgacttagct aaagcactac gacctgagtc agattctaag
    21601 attgcttggg caatcatgac agacgcataa atatcgttat cttgaccaat gcgatgtgca
    21661 tctttagcaa ttgatttgac aaattgacgt gtatcttttg agtcaacaac gttaaattgt
    21721 ccgctatcat cattgttaga tatactagga tctgtttcga ataatgatgt tgcacgtgta
    21781 tccttttgat taacatcgtt attgaatgat tgagcaggtt tagatttatg tttcaattca
    21841 tcttgtgttg gtaactgtgg attctttgta ttagattttt catttttgtc ttttttagat
    21901 tgagatgcat aatctttttg tgctttcttt gcatcttcac tgtattgatc caaaatagag
    21961 tctaaagccg aatctgacat tgattgatta tctttcgatg aagatttttg atttgcttta
    22021 tcgtcacttg ctggttgact atttgattga ttaggttgtg ttggctttgg cgaatttggt
    22081 tgcttattag atgtacttgg ttttgtattg tttgatttag gtgctttttg attgtctgct
    22141 ttatcttgtt tagatgattg cgtatcagtg tcatttttga tgctattgtc actgttttta
    22201 ttcgaatcat ttgttgactt ttcgccatta cgaggttgtt cgtaatcaga aatatccgaa
    22261 tttaaattga ataagttttg gattaaagtt gttaatgagt aattatcatc gtatttattt
    22321 ttggttagca attggtttat attggtttgt ggtaaattct tataaataaa atcaatgata
    22381 ttgttagagt ctgaagtgct gtcgtctata gttttaaatt ttttgtcgtt attgtcttgg
    22441 ttacttgtat tatttttgtc tgctttatca atatctttac ttgtagtatc cttagaagtt
    22501 tcatcgtcat tagatttttt tgaatcatga gatgttgtct tagctgtagt atctttttga
    22561 ggtgtatcag cataagcggt aggtgaaact aaagtaggta atacgagcgt agttgatagc
    22621 aaataaatta aaattttatt tttaggcata tttcgtattc tcccttgaaa aatataataa
    22681 ttaagtgtga taataaacta tgatttgtta taatttatcg tatgctgaaa atagttgata
    22741 ggtatcaatc gactaaatat cttccagtaa attgattata ctaattcaca acgcaaaaat
    22801 aaattaattt acaaaaaata tataaaaaat atgaataatt cctacatagg agtgtgacaa
    22861 tgaagaacgc atttaaatta tttaaaatgg atctgaagaa agtagctaag acgccagctg
    22921 tgtggattat cttagcaggc ttagctattt tgccatcgtt ctacgcttgg tttaacttat
    22981 gggcaatgtg ggatccatat ggcaacacgg gacacatcaa ggtcgcagtc gttaatgaag
    23041 ataaaggcga cacaatcaga gggaaaaaag ttaatgtcgg taatacgatg gttaatacac
    23101 tcaagaaaaa taaaagtttt gattggcagt ttgtaagtag agagaaagct gatcatgaga
    23161 taaaaatggg taaatatttt gcaggtattt acatcccatc taagtttaca catgaaatta
    23221 cagggacact acgtaagcag cctcaaaaag cagatgtaga atttaaggtg aatcagaaga
    23281 ttaacgctgt tgcgtctaag ctaacagata ctggttcgtc agttgtcgtt gaaaaagcga
    23341 atgaacaatt taataaaaca gtaactcgag cattattaga agaagctaac aaagcaggtt
    23401 taactattga agaaaatgtg ccgacaatta acaagataaa aaatgcggta tattcagcag
    23461 ataaagcttt acctaagatt aatgactttg cgaataaaat tgtatatttg aataaccacc
    23521 aagcggattt agataaatat gccaatgatt ttagaaaact aggaaattat aaaggtgata
    23581 ttttagatgc tcagaaaaaa ttaaacgaag tcaatggtgc tattccgcaa cttaatgaaa
    23641 aggctaagtt gatattagct ttaaataatt atatgccgaa aattgaaaaa gcgttaaatt
    23701 ttgcagctga tgacgtgcca gcgcagttcc ctaaaattaa tcaaggactt aacattgcga
    23761 gtcaaggtat tgatcaagct aatggacagt taaatgatgc caaaggcttc gtcacacaag
    23821 ttagaagtag agtcggtgat tatcaagatg caattcgacg cgcgcaagat ttaaatcgaa
    23881 gaaaccagca acagattcct caaaatagcg cggcgaacaa cgaaacatca aatagtgcac
    23941 ctgcagctgg taatggtgta gcatcaacgc caccaagtgc accaagtggc gatactgcac
    24001 caaataataa tgttacgcaa aataccgcac caaatagtaa taatgcgcct gtatcgacta
    24061 caccacaaag tacaagcggg aaaaaagatg gtcaaagttt tgtagatata acaacaacac
    24121 aagtcagcac agctaacgag aacacacaaa acattacaga taaagatgtt aaatcaatgg
    24181 aagcggcatt aacgggctct ttattatcat tatcaaataa tttagatacc caagcgaaag
    24241 ccgcacaaaa agatagtcag gcattacgta atatttcgta tgggatttta gcatcggaca
    24301 agccttctga ttttagagag tctttagata atgttaagtc cggtttagaa tacacaacgc
    24361 aatataatca acaatttatc gatacattaa aagagattga gaagaatgaa aatgttgatt
    24421 tatcaaaaga aattgataag gtaaaagcag ctaataatcg aattaatgaa tcattaaggt
    24481 tagttaatca attaagcaat gcattaaaga atggtagttc aggaactgct gaagctacta
    24541 aattactaga tcaactttca aaactagatt catcattatc atcatttaga gattatgtta
    24601 aaaaagatct taacagctct ttagtatcaa tatcacaacg tattatggat gaattgaaca
    24661 aagggcaaac tgcattatcc aatgttcagt ctaaattaaa tacaattgat caagtcatta
    24721 acagtggaca agctatttta aaaaatggta aaacacgtat tgatcgctta caaacagtat
    24781 taccaagtat tgaacaacaa tacattagtg ctgttaaaaa tgctcaagca aacttctcga
    24841 aagtgaaaag tgatgtagct aaagctgcta actttgtgcg caatgactta ccacagttag
    24901 aacagcgatt aactaatgcg acagcaagtg tgaataaaaa tttaccaacg ttattaaatg
    24961 gttatgatca agcggtagga ttactaaata aaaatcagcc acaagcgaaa aaggctttat
    25021 cagatttagc tgatttttct caaaataaat tgcctgatgt tgaaaaagat ttgaaaaaag
    25081 cgaataaaat tttcaagaaa ttagacaaag atgatgcagt cgacaaatta atcgacacac
    25141 ttaagaatga tttgaaaaag caagcgggta ttattgcaaa tcctattaat aagaagactg
    25201 ttgatgtttt cccagttaag gattatggtt caggtatgac accattctat actgcactgt
    25261 cagtatgggt aggtgcactc ttgatggtaa gtttattaac ggttgataat aaacataaga
    25321 gtctagagtc agtcttaacg acaagacaag tgttcttagg taaggcagga ttctttataa
    25381 tgcttggtat gttgcaagca ctcattgtat cggttggaga tttgttaatc ctaaaagcag
    25441 gagttgagtc acctgtatta tttgtactta taacgatttt ctgttcgatt attttcaact
    25501 caatcgtata tacgtgcgta tcattacttg gtaacccagg taaagccatt gcaatcgtat
    25561 tgcttgtatt acaaattgca ggtggtgggg gaacattccc aattcaaact acgccacaat
    25621 ttttccaaaa catttcgcca tacttaccat ttacgtatgc aattgattca ttacgtgaaa
    25681 cagtaggcgg tattgttccg gaaatcctaa ttacaaaatt aattatatta acgttatttg
    25741 gtataggatt cttcgttgta ggtttaattt taaaacctgt aacagatcca ttgatgaagc
    25801 gcgtatctga aaaagttgac caaagtaacg ttacagaata aaaattaaat ccacacatta
    25861 gggttatagc tccttaatgt gtggattttt atgtttttag acagaagaga tagtaatttc
    25921 tgtcttttat gggacggttg ttatcattgc tattatccag gatgacttac tatagcacta
    25981 atattaccga caaagtgaat atcctcgtct tccgtagtta aaataaagct agaacctttt
    26041 tggatgtcat agtgcttatc gtttactgtt aaagtaccag taccatcgat aattgtaact
    26101 aagcaataag catgtggttt attgaatttt aaatctccat gaatatccca tttatatact
    26161 gcaaaatatt gattatctac aaattgagtt acagtgtgtg tgtcgatgtg agttgttata
    26221 ggagtagtat ttggttcatg attgcctaat tcaatcacat ctttactttg ctctaagtgc
    26281 aaatcacgca attgaccatt ttgatctcgt ctatcatagt cataaatacg gtatgtcgta
    26341 tcggaggatt gttgtgtctc taaaattaaa atacccgaac caatggcatg gacagtgcca
    26401 gcaggaacat aataaaagtc accgggctta acaggtatac gtttgaaaag actgtcaaat
    26461 tcatgattat caatcatgtc tattaacgtc tgtttattat gtgcatgtac gccatatata
    26521 atttcagcac ctggggctgc atctaaaata taccaacatt ctgttttacc tagttcgcct
    26581 tcgtgtttta aagcgtagtc atcatctggg tgaacttgaa cagataattt atcattggca
    26641 tctaatactt tagttagcag agggaaacta tctcgtgaat cattatcgaa taattcacga
    26701 tgttgtgacc aaagttgatc tagggtcata tccttgtatg gaccattgat aattgtatta
    26761 ggaccatttg gatgtgcaga aattgcccag cattcaccag ttgtttcatt agggatatca
    26821 tagttaaatg cttttaatgc atgaccgccc caaattctgt ctttaaaaac gggttgtaaa
    26881 aataatgcca tagttaaaac tcctctatat tttcattaat aagttataaa tttctgtagt
    26941 actgtttgca ttaattagtg attggcgtgt ctcatcattc attaacgctt tagataagcg
    27001 ctgaagtatt tttaaatgtg tatcctgact gttgtttggt acggcaatta agaatatcaa
    27061 ttgaggtaga ctaccatcta gactgtccca tttaacacca tgattatttt tcataacagc
    27121 tacaatcggt tgttttacaa catcagactt tgcatgtgga atggccacgt tcatgccaat
    27181 agctgtcgta gaccccattt cacgttctag tattgcattt tttaaatgcg atgtgtgctc
    27241 tacataacgg caaattttaa gtttatgaat caacatatca attgcttcgt ttcgagacat
    27301 gtcgtgatca gtaattatca tagtttgttg atcaaaaaca tgagaaggtt tattgagatg
    27361 tgaatgtttc gcggtgttat ctacattgtc aacctctgta tcatgttgtg taatatctgt
    27421 atcatgaagt tgcgtgtgtt gcgctggtgc atctactgct ataactggtg tattgcgttt
    27481 taataatagt acagtagtca ttgtgacaag actacctact atcactgcaa agataaacca
    27541 taatacatga tcaataccac ctaatacagc cacgattgga cctccatgtg cgactctatc
    27601 gccgacacca ccaatggctg caatgactga tgcaatcatt gcaccaatga tgtttgcagg
    27661 tataatgcgc aatggatctt gggctgcgaa aggaatagca ccttcagtaa taccaaatag
    27721 tcccatagtg aaggaagcct tacccatttc tctttcggaa tgattgaatt tatacttttg
    27781 aacaaacgtt gctaaaccta aaccgattgg tggtgtacat acagcaactg cgaccatacc
    27841 cataacggcg taattacctt cagcaataag tgctgagcca aataaaaatg ctaccttgtt
    27901 tactggaccg cccatatcga aggcaatcat cgcacctata atcatcgcaa gtataataat
    27961 attagcacct tgcatacttt ttaaccaggt tgttaatgcc tcaaaaatat tagaaattgg
    28021 tgcaccgatt aaaaatataa atatcaatcc tacaacgacc gatgaaataa tgggaataat
    28081 aatgataggc ataattggtg ccattgcttt tggaacttta atatctttaa tccactttgc
    28141 gatataacct gctaagaaac cagcaacaat accacctaaa aatcctgcgc ctgcatcact
    28201 gccataaaaa ctaccgtcag cagcgatagc gccgccaatc ataccaggaa caagaccggg
    28261 cttgtcagcg atactaacag cgatatatcc agctagtatt ggaaccataa atttaaaggc
    28321 taaactacca atgttttcaa tggatttcca aaatgaatca tctgggatga ctaatccttt
    28381 tgatgtcgtt tcaccgccta gagtcagcgc gatggcgata aggagtccac caactacgat
    28441 aaaaggaacc ataaacgata caccgttcat taaatgttga tacaccattt gaataccact
    28501 tttagactta ccgcgatctt tcgaatgata atttgtttca gattgataaa taggcgcatc
    28561 ttgattaatg atacgttgaa ttagacctct cggattatga atcccttcgc gaacattttc
    28621 attaatcaac cgtttaccaa caaatcggga cagatcaact tgtttatcag ctgcaattat
    28681 gacaccgtca gcttcttcga tgtcttgcgt agttaaaaca ttttcagcac caacaccgcc
    28741 ctgtgtctct actttaatat ccacacccat ttcttttgct acctgctcaa gcttttcttg
    28801 agccatatat gtatgtgcaa tgccatttgg gcatgaggta atagctacaa ttttcataaa
    28861 atcatctcct tttctatatt gtaagcgtat tctcgatact aaaaaaaaga ataattaccg
    28921 ttactagtgg caattattct tgtaagtatt caaataactg ttgctttaaa ctatgatcat
    28981 ctaaactaca taaatggttc actgaatcat catccaagtt agcaattaat tgcatcattt
    29041 gttttgtaaa agctttgtct ttatgcgaaa tcgctaagaa aaagacaagt ttgacatcgt
    29101 gttgtcgcca aggaaaaaca tcttttgtgc gaaaaataag cacatgtgat tgtaaaactt
    29161 tttcaggatc tccatgagga atcgccataa aattacctat gtatgtagaa gatgatttct
    29221 cacgctctaa agctgattcg atatatcctt ctacaatcgc atgatgtgct tgtaatattt
    29281 tttgagcttc ttcaaaaatt tgcacagtat gccgtgattt ttgttcagta tttacgacaa
    29341 ggaaattgac agtgtccata tgatgatgtg cttgaaccgg attttgcttt tgcttcacaa
    29401 cgtgtctgat tttgtgacga tcatcttcag aaaataatgg tgcaaccttg atagtcgtca
    29461 ggtgcttagg aagtatgttt agcgtttgtt taggaatatc atgggtcgtt attaataaat
    29521 ctacattgtc aaagtgatag tgtgttatat tttctagttt aatcgtattt atcactgaca
    29581 actcttcgga taagttattt attttagttt ctaaaaaatt cgacacacct agaccataat
    29641 aacaagcaat gactacattt aattgtgttt tggtacgacg ctcgatggca gcttgaaaat
    29701 gaattgttaa aaatgcaatt tcatcttcgc tcatctctat atcagtatca attgctaatt
    29761 tatcaatcgc ttcaaaaagt gtgttaaaca caaagggata gagtttttta atctctataa
    29821 ctaaaggatt gtttaaataa atgttttgag tgatacgtaa atatgcttta ctaaaatgat
    29881 tatataaatt ttgttgtaaa atcgaatctt cattgaaagg tacatgaata cgttgctgca
    29941 tcaattcgat taagcgatca atataacttt gtataaatat acgttctatg ccaatatcga
    30001 gtttattaaa atgataagca ataaagaatg aaaacatatt gattactttt tcgttcaagt
    30061 cataacctaa tctttcgttg atttgcttaa tgcaagattg agatatcaat tttgaatcgt
    30121 ggtttaacgt gtctagctca cttaaagatt gcttacgttt aatgattaaa atgagatgaa
    30181 ttaaaagctg ttgtatttga atatcagttg tttcaatact atgttgttga agtgtctctt
    30241 gtataatatg cgaaatcatc ctttggtgtg aatcaggtaa ttcatttaaa attaggtctt
    30301 caacatgtac atgccctgat gataattgat ttaaatggat gatggcatta gtgatatcat
    30361 tatctgttcc atcgaccaca ataccttgtt ttttcttgat tgttaaagcg atattaaatt
    30421 tttgacacca ttgttgaatt cgaacgaaat agtcgttgag cacagtttta gataatagat
    30481 aatcttgctc tagttgttgc aatgtctgcg atttattgtg cattagcagt tgatacgcca
    30541 tagataataa tagtttttct ttatatgatt gaatatgcgt gtaacgttca gttatcgttt
    30601 ctaaggtata ctgagatgta tttaattgat atcctttaga tttaatactt acgatgatat
    30661 catccataaa attgctattg ataacatgga tatcattgcg aactgtgcgg tttgaaacat
    30721 tgacatgttt agcaatttca ttagaactaa tgtgctttga tggattttta ataaaaaact
    30781 ggagtagttt taagtgtcta tcaagcattt aacatgtacc tcctttctaa atttttcgtg
    30841 taagcgtttt taagggtgta ttaatattat taaacatgag agcttataca tacgtcaatg
    30901 acattaaagc gaacttttat atgattttaa cagagtgcga attatgcaaa taaagaacag
    30961 cagtaagata tttcaaatag aaaaatatct cactgctgtt tttctgaatt tatgcatctg
    31021 gtacttgtgg acgtatcagg caaatgatta atttttaggt gattgtgctt gaggtgtttg
    31081 tttagaaggc gtattggtat tatttgattt attagcaggt ggtgtgttct gttgttgatt
    31141 attttggttg tttgttgacg attgactgcc accaccgtga gtattatttt tatttgaatt
    31201 tgagtctttt ggctgagctg gttgcgtatt cgatggtact gttgaatggt tgttaggaac
    31261 atttgacggt gaagatggtt gattgtcatt agtaacgtta ttgccattat tttgattttt
    31321 atctgttttg tctgcactat catcttgttg atcattttct tcggtttctg tctttttatg
    31381 cgtagattta ttttcttgtt cttggttatt acttgatgat gtagaatgat ttattgcagc
    31441 ataaattgcg aacccaagtg ctccgattaa tacgataatt acgatggtaa taagaagttt
    31501 tttcaaatat aacgcctcca tgttaatgaa aagtagttga tacgtgaagc taaataattt
    31561 atttcaatat tgccataatc attttaaaaa ataaatatta gaaaagtagg cgatattaat
    31621 attttcattg ttttttgtgt acttcaatta gtataac
//
LOCUS       DALAAR010000030        30163 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000030 DALAAR010000000
VERSION     DALAAR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.030"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(125..964)
                     /gene="noc"
                     /locus_tag="O2458_001366"
     CDS             complement(125..964)
                     /gene="noc"
                     /locus_tag="O2458_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCZ3887233.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="O2458_001367"
     CDS             complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="O2458_001367"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCZ3887234.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="O2458_001368"
     CDS             complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="O2458_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCZ3887235.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="O2458_001369"
     CDS             complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="O2458_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCZ3887236.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="O2458_001370"
     CDS             complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="O2458_001370"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCZ3887237.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="O2458_001371"
     CDS             complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="O2458_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCZ3887238.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6530..7891
                     /gene="dnaA"
                     /locus_tag="O2458_001372"
     CDS             6530..7891
                     /gene="dnaA"
                     /locus_tag="O2458_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCZ3887239.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8169..9302
                     /gene="dnaN"
                     /locus_tag="O2458_001373"
     CDS             8169..9302
                     /gene="dnaN"
                     /locus_tag="O2458_001373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCZ3887240.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            9683..9928
                     /gene="yaaA"
                     /locus_tag="O2458_001374"
     CDS             9683..9928
                     /gene="yaaA"
                     /locus_tag="O2458_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCZ3887241.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            9925..11037
                     /gene="recF"
                     /locus_tag="O2458_001375"
     CDS             9925..11037
                     /gene="recF"
                     /locus_tag="O2458_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCZ3887242.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            11047..12981
                     /gene="gyrB"
                     /locus_tag="O2458_001376"
     CDS             11047..12981
                     /gene="gyrB"
                     /locus_tag="O2458_001376"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCZ3887243.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            13018..15681
                     /gene="gyrA"
                     /locus_tag="O2458_001377"
     CDS             13018..15681
                     /gene="gyrA"
                     /locus_tag="O2458_001377"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCZ3887244.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(15768..16580)
                     /locus_tag="O2458_001378"
     CDS             complement(15768..16580)
                     /locus_tag="O2458_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCZ3887245.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            16906..18420
                     /gene="hutH"
                     /locus_tag="O2458_001379"
     CDS             16906..18420
                     /gene="hutH"
                     /locus_tag="O2458_001379"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCZ3887246.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    18493..18709
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18799..20085
                     /gene="serS"
                     /locus_tag="O2458_001380"
     CDS             18799..20085
                     /gene="serS"
                     /locus_tag="O2458_001380"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCZ3887247.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            20735..21430
                     /locus_tag="O2458_001381"
     CDS             20735..21430
                     /locus_tag="O2458_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCZ3887248.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            21427..21756
                     /locus_tag="O2458_001382"
     CDS             21427..21756
                     /locus_tag="O2458_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCZ3887249.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     regulatory      21961..22059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22119..23087
                     /locus_tag="O2458_001383"
     CDS             22119..23087
                     /locus_tag="O2458_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCZ3887250.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            23378..24316
                     /locus_tag="O2458_001384"
     CDS             23378..24316
                     /locus_tag="O2458_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCZ3887251.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            24331..26298
                     /gene="gdpP"
                     /locus_tag="O2458_001385"
     CDS             24331..26298
                     /gene="gdpP"
                     /locus_tag="O2458_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCZ3887252.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            26295..26741
                     /gene="rplI"
                     /locus_tag="O2458_001386"
     CDS             26295..26741
                     /gene="rplI"
                     /locus_tag="O2458_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCZ3887253.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            26773..28173
                     /gene="dnaB"
                     /locus_tag="O2458_001387"
     CDS             26773..28173
                     /gene="dnaB"
                     /locus_tag="O2458_001387"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCZ3887254.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            28452..29735
                     /locus_tag="O2458_001388"
     CDS             28452..29735
                     /locus_tag="O2458_001388"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCZ3887255.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
BASE COUNT        10218 a         4856 c         5514 g         9575 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaaaagac
       61 aaagctgtta tgatcttagc tttgtctttt atgtgttagt catcatgtat acgacatcct
      121 actactaacg tttatatatt cgaattttta tttcataata atcttcatga tctttgtctt
      181 tatgctcaac atgtaatcct gtttgttgaa tcgcttggat acttttgcct acctcatctc
      241 gtgcttgagt gacatcttgt gcaaagcgaa ggttttgtgc tttgactttt tcgggccccg
      301 ttttttggcg tactctatct tcagtctgtt tcacatttag cttttgtgca atgacttgct
      361 caatcaacgc ttcttgttcg ctatcagaca atgataatac cgctctcgca tgacgttcag
      421 taattttacc ttctcttaag cgaagtagta ctttcggcgc caacttcaat aaacgcaact
      481 tatttgcaat aaagctttga cttttaccta aactttttgc caattcactt tgcgttgtat
      541 caccaatttc caataatttc ttataggctt ccgcttcttc aacaacagac aaattttctc
      601 tttgaatatt ctcaattaat gcaacaacag ccgtctcttc atcatccata tcacgaataa
      661 taacgtctgc ttgaggtaaa tttagtgatt gtattgctct aaatcggcgc tctccagcaa
      721 taatttcaaa catatcttct tcaatcggtc ttacaacaat aggttgtagt aaaccatgtt
      781 catgtattga ttcagcaagt tctttaattt tatttggttc aaacacctgt cttggttgat
      841 aacggttggg aacgatacgt tcaatttgaa tggattcaac attactattg cgatcttctt
      901 caatatgtcc aatgatgtca tctttgtttt tcaaaccaaa taattttgaa aaaggttttt
      961 tcattatcca ttcgctcctt ttatgttagt tgtaaaggat tatgcattat ttttcaagta
     1021 aaggagtctt attaggcgtc cctggttttc ttggatactt tttcggcgtc tgtctttttt
     1081 tatcaataat gaacatctgg cgctctccag catcttctgg caattcaaag gtatgtgttt
     1141 ctgtaacatt accacctaac acactaattg caaattttgc ttcttctaat tcttcttcac
     1201 cttttgaaga ttttaatgca acaaactgtc cacctttttt aactagcggt aaacacaatt
     1261 cacttaacac ggataatcta gctactgctc ttgcagtaac aacatcataa gactccctgt
     1321 agacaccctt accaaatgtt tctgctctat cgtgtataaa gctgacatcc tgtaattgta
     1381 attctgacgc taaatggttt aaaaattgaa tacgcttatt taatgaatca acaatcgtca
     1441 cttttaactg cggaaacatt atttttaacg gaatacttgg aaaaccagct ccagcgccta
     1501 catcacatat acttataggc tgattaaaat caaaataaaa actaggtgca atggaatcat
     1561 aaaaatgttt caaatataca tcgtgttcat ctgtaatact tgtcaaattc atcttttcat
     1621 tccattcaac aagtaaacga taatatgttt gaaactgttg tttttgagtc tcagttaatt
     1681 gaatattatg ttcttttaat tgttctgcta accattctac agtcattagt cactcaccct
     1741 ttggagttta ccttgttcta agtaaatcaa taatatagat atgtctgctg gatttacccc
     1801 tgatattcta gaagcttgtg caatatttaa aggttttact tctgataatt tttctcgcgc
     1861 ttcagtcgcc aaactatcaa tcttactata atctaagtct tctggaattt tcttctcttc
     1921 catacgctta actttctcaa cttgttgtag tgatttattg atataacctt catattttgt
     1981 ttgtatttct acttgttctt caacatctgc attcaattga tgttcttctt ctaaaagttc
     2041 taaaattata tcgtaagtca tttcaggtct gcgtaataaa tcgatagcta aaataccatc
     2101 ttttaagcga gaaccaccat gttgttcaat aatcgcttgc gtatgttcgt ttggtttaat
     2161 acgaatatct gataaacgct taatttccgc atcaatttgc tgacgttttt cattaaaacg
     2221 tgcatatctt tcttcagaaa tcataccaag ttcatatccc atatccgtca atctcaaatc
     2281 agcattatca tgacgtagta acaaacgata ttctgcacgt gatgttagta aacgataagg
     2341 ttcattagta cctttagtta caagatcatc gattaagaca ccaatatatg catctgaacg
     2401 acttaatatc ttttcgcctg tgtttaacac tttacctgca gcgttaatac ctgccatcaa
     2461 tccttgtcct gctgcttctt cataaccaga tgtaccatta atttgacctg cagtatataa
     2521 gtttttaatc attttcgttt caagtgtagg ccataactgc gttggcacaa tcgcatcata
     2581 ttcaattgcg tagccggcac gcatcatatc tgctttttca agacctggta tcgtctctaa
     2641 catttgacgt tgcacatgtt caggaagact tgtagacaat ccttgcacat atacttcatt
     2701 tgtattacga ccttcaggct ctaagaaaag ttgatgtcgc ggcttatcat taaatcgaac
     2761 aaatttatct tcaattgaag ggcaataacg tggcccggtt cctttaatca tccctgaata
     2821 cattgcagat agatgtaaat tatcatcgat aactttgtgt gtttcagcat tagtatacgt
     2881 tagccaacat ggcaattgat ctaatatata ttctgttgtt tcaaagctga atgcacgacc
     2941 tacatcgtca cctggttgta tttcagtctt cgaatagtca attgtttttg aatttacacg
     3001 cggtggtgta cctgttttaa aacgaacaat atcaaaacca agttctctta aattgtctga
     3061 taatgtgatt gatggtaatt ggtgatttgg tccacttgaa tacttcatat tacctaaaat
     3121 gatttcacca cgtaaaaatg ttcccgttgt aataattact gctttagata aatactctgt
     3181 accaatattt gtacgtacac ctttaacttc attatcttct ataataagtt cgtctaccat
     3241 accttgcatt atatgcaaat tttcttcatc ttcaatcacg cgtttcattt cttgttgata
     3301 aagtacttta tctgcttgcg ctcttagtgc tcttacagca ggtcctttac ctgtatttaa
     3361 cattctcatt tgaatgtgtg ttttatcgat tgtttttgcc atttgtccac ctaaagcatc
     3421 aatttcacga acaacgatac ctttagctgg tccacctaca gatgggttac atggcataaa
     3481 tgcaatatta tctaaattta ttgttagcat taatgtttta gcaccacgtc ttgcagatgc
     3541 taaacctgct tctacacctg catgtcccgc acctataacg attacatcat attcttgaac
     3601 cacaatataa acctccttat ttgatatctt actagccttc ttaagacggt attccgtcta
     3661 tttcaattac tatttaccta agcagaattg actgaataac tgatcgatga gttcatcact
     3721 tgcagtctca ccaataattt ctcctaatat ttcccaagtt ctagttaaat caatttgtac
     3781 catatccata ggcacaccag attctgctgc atcaatcgca tcttgtatcg tttgtcttgc
     3841 ttgttttaat aatgaaatat gtcttgaatt agaaacataa gtcatatctt gattttgtac
     3901 ttctccacca aagaacaaat ctcgaatttg tatttctaat tcatcaatac cttcttgttt
     3961 taacattgaa gtttgaatta atggcgtatc acctatcata tctttaactt cattaatatc
     4021 tatgttttgc tctaaatcca ttttattaac aattacgatt acatcttcat ttttaaccac
     4081 ttcatataat gtgtaatctt cttgagtcaa tgcttcgtta ttgtttaata caaataaaat
     4141 taagtctgct tggctaagag cctttctaga gcgttcaaca ccaatcttct ctactatatc
     4201 ttctgtctca cgtataccag cagtatcaac taatcttaat ggcacgccac gaacattgac
     4261 gtactcttct aagacatctc tagtagtacc tgctacctca gttacaatcg ctttattatc
     4321 ttgtattaaa ttatttaaca tcgatgattt acctacgttt ggtttaccaa caataactgt
     4381 agataaacct tcacgcataa ttttaccctg cgcaccggta tctaataaac gattaatttc
     4441 ctgtttgatt tctttagact gctctaaaag aaattcagta gtcgcatctt caacatcatc
     4501 gtattcagga taatcaatat tcacttccac ttgagcgagt atctctaata tagattgacg
     4561 ttgttttttg attaagtcac ttagacgacc ttcaatttga ttcatcgcaa ctttagaagc
     4621 tctatctgtc ttcgagcgaa taaagtccat aactgcttca gcttgagata aatcaatacg
     4681 accatttaaa aaggcacgtt ttgtaaattc acctggctca gccattctag cgccatatgt
     4741 catagtaagt tccagcactc tattaatcgt taaaatacca ccatgacaat taatttctat
     4801 aatatcttcg cgtgtaaatg tttttggcgc tcttaacaca gacaccataa cttcttcaat
     4861 cacttcttta gactctggat caataatatg accgtaatta atcgtatgtg atggaacatc
     4921 atttaaaaga tgttttcctt tatataattt gtcagcaatt tcaacggctt gcggtccaga
     4981 caatcgaaca attccaattg ccccttcacc cattggtgtt gaaatactcg taattgtatc
     5041 taaatccata ttgctactcg cctccttcaa cgatgtgaat acattttaaa gtaagttatt
     5101 ataaccctaa ggtcagtctt acgtttgtct gaggtaagac ttcgggatgt gttgagtggt
     5161 taatgttttc cttcccctac cctatcctta cttaatcttt ttattaaaaa ctttggcaat
     5221 tttaagtacg tgctcaagac tattctgtat ttgtaaagtc gtcatatctt tagctggctg
     5281 tcttgctatt acaataatat ctttggccaa tatatgcgac ttatgtactt tgaaattttc
     5341 acgtattgct cttttaatct tgtttcttaa cactgcatta cctagttttt tagaaacact
     5401 aatacctaag cgaaaatggt ctatttcttt attattacaa gtgtatacaa caaattgtct
     5461 gttggctaca gaatgacctt ttttatatat tctctgaaaa tctgcattct ttttaattcg
     5521 gtaagctttt tccaataaca tcactcgctt atttatcgtt tttatttgaa gctatattta
     5581 aacttctatt gagcttataa cataaatttc tatttattct taatttaaac gaaaaaaaag
     5641 atcactgatg agtcagtgat cttatgcaga taaaacttta cggcctttac gacgacggcg
     5701 cgctaaaact ttacggccat tttttgtgct catgcgtttt ctgaaaccat gaactttact
     5761 atgtttacgt ttatttggtt gataagtacg ttttaccatg caaaacacct ccatctttat
     5821 acagttatct aatataccag attatgcaga ctctgatcac tttaatagat aagattttgc
     5881 gtttcttaga ttaaaattct cgtattagct cattgattat ctagtcataa ttcaagcaac
     5941 tactacaata taacaaaatc ctatttataa cgcaagttca ttttatacta ctgctcaatt
     6001 tttttacttt tatcgattaa agatagaaat acacgatgcg agcaatcaaa tttcataaca
     6061 tcaccatgag tttggtccga agcatgagtg tttacaatgt tcgaacacct tatacagttc
     6121 ttatacatac tttataaatt atttcccaaa ctgttttgat acactcacta acagatactc
     6181 tatagaagga aaagttatcc acttatgcac atttatagtt ttcagaattg tggataatta
     6241 gaaattacac acaaagttat actattttta gcaacatatt cacaggtatt tgacatatag
     6301 aggactgaaa aagtataatt gtgtggataa gtcgtccaac tcatgatttt ataaggattt
     6361 atttattgat ttttacataa aaatactgtg cataactaat aagcaagata aagttatcca
     6421 ccgattgtta ttaacttgtg gataattatt aacatggtgt gtttagaagt tatccacggc
     6481 tgttattttt gtgtataact taaaaattta agaaagatgg agtaaattta tgtcggaaaa
     6541 agaaatttgg gaaaaagtgc ttgaaattgc tcaagaaaaa ttatcagctg taagttactc
     6601 aactttccta aaagatactg agctttacac gattaaagat ggtgaagcta tcgtattatc
     6661 gagtattcct tttaatgcaa attggttaaa tcaacaatat gctgaaatta tccaagcaat
     6721 cttatttgat gttgtaggct atgaagttaa acctcacttt attactactg aagaattagc
     6781 aaattatagt aataatgaaa ctgctactcc aaaagaaaca acaaaacctt ctactgaaac
     6841 aactgaggat aatcatgtgc ttggtagaga gcaattcaat gcccataaca catttgacac
     6901 ttttgtaatc ggacccggta accgctttcc acatgcagcg agtttagctg tggccgaagc
     6961 accagccaaa gcgtacaatc cattatttat ctatggaggt gttggtttag gaaaaaccca
     7021 tttaatgcat gccattggtc atcatgtttt agataataat ccagatgcca aagtgattta
     7081 cacatcaagt gaaaaattca caaatgaatt tattaaatca attcgtgata acgaaggtga
     7141 agctttcaga gaaagatatc gtaatatcga cgtcttatta atcgatgata ttcagttcat
     7201 acaaaacaag gtacaaacac aagaagaatt tttctatact tttaatgaat tgcatcagaa
     7261 taacaagcaa atagttattt cgagtgatcg accaccaaag gaaattgcac aattagaaga
     7321 ccgattacgt tcacgctttg aatgggggct aattgttgat attacgccac cagattatga
     7381 aactcgaatg gcaattttgc agaagaaaat tgaagaagaa aaattagata ttccaccaga
     7441 agctttaaat tatatagcaa atcaaattca atctaatatt cgtgaattag aaggtgcatt
     7501 aacacgttta cttgcatatt cacaattatt aggaaaacca attacaactg aattaactgc
     7561 tgaagcttta aaagatatca ttcaagcacc aaaatctaaa aagattacca tccaagatat
     7621 tcaaaaaatt gtaggccagt actataatgt tagaattgaa gatttcagtg caaaaaaacg
     7681 tacaaagtca attgcatatc cgcgtcaaat agctatgtac ttgtctagag agcttacaga
     7741 tttctcatta cctaaaattg gtgaagaatt tggtgggcgt gatcatacga ccgtcattca
     7801 tgctcatgaa aaaatatcta aagatttaaa agaagatcct atttttaaac aagaagtaga
     7861 gaatcttgaa aaagaaataa gaaatgtata agtaggaaac tttgggaaat gtaatctgtt
     7921 atataacagc actaatgata acaatcattt tttacatttc tatatgctaa tgtggcaaga
     7981 tgagcaaaac tcattttgtg gataatgttt aaaagtcata cacaccatac acaagttatc
     8041 aacatgtgta taacttcgcc aaatctatgt ttttaagact tatccaccaa tccacagcac
     8101 ctactactat tactaagaac ttaaaaccta tataattata tataaacgac tggaaggagt
     8161 tttaattaat gatggaattc actattaaaa gagattattt tattacacaa ttaaatgaca
     8221 cattaaaagc tatttcacca agaacaacat tacctatatt aactggtatc aaaatcgatg
     8281 cgaaagaaca tgaagttata ttaactggtt cagactctga aatttcaata gaaatcacta
     8341 ttcctaaaac tgtagatggc gaagatattg tcaatatttc agaaacaggc tcagtagtac
     8401 ttcctggacg attctttgtt gatattataa aaaaattacc tggtaaagat gttaaattat
     8461 ctacaaatga acaattccag acattaatta catcaggtca ttctgaattt aatttaagtg
     8521 gcttagatcc agatcaatat cctttattac ctcaagtttc tagagatgac gcaattcaat
     8581 tgtcggtaaa agtgcttaaa aacgtgattg cacaaacaaa ttttgcagtg tccacctcag
     8641 aaacacgccc agtactaact ggtgtgaact ggcttataca agaaaatgaa ttaatatgca
     8701 cagcgactga ctcacaccgc ttggctgtaa gaaagttgca gttagaagat gtttctgaaa
     8761 acaaaaatgt catcattcca ggtaaggctt tagctgaatt aaataaaatt atgtctgaca
     8821 atgaagaaga cattgatatc ttctttgctt caaaccaagt tttatttaaa gttggaaatg
     8881 tgaactttat ttctcgatta ttagaaggac attatcctga tacaacacgt ttattccctg
     8941 aaaactatga aattaaatta agtatagaca atggggagtt ttatcatgcg attgatcgtg
     9001 cctctttatt agcgcgtgaa ggtggtaata acgttattaa attaagtaca ggtgatgacg
     9061 ttgttgaatt gtcttctaca tcaccagaaa ttggtactgt aaaagaagaa gttgatgcaa
     9121 acgatgttga aggtggtagc ctgaaaattt cattcaactc taaatatatg atggatgctt
     9181 taaaagcaat cgataatgat gaggttgaag ttgaattctt cggtacaatg aaaccattta
     9241 ttctaaaacc aaaaggtgac gactcggtaa cgcaattaat tttaccaatc agaacttact
     9301 aaaaataaat ataaataaag gatgacgtga ttaattaaaa cgtcatcctt tattttttgg
     9361 caaaaataat tctaggtgcg tatgtaaaat aaatttggca gcattttaaa cagcaaataa
     9421 aagacgccaa ttaaatttat gacaaatgta tccaaaattt aataagtgtg cttatatgcc
     9481 ctttaaattt aaaattttaa tagtcaataa caagttgaat ataaaagtta aacgccgtta
     9541 aatagcgtta aaaaattgaa aatgacagta ttgccaaaaa ataagaatta attatttata
     9601 tgtaaacggt ttctacctct attttaaatg aaatttgtga caaaaaaagg tataatatat
     9661 taatgacata caaagaaatg gagtgattat tttggttcaa gaagttgtag tagaaggaga
     9721 cattaattta ggtcaatttc taaaaacaga agggattatt gaatctggtg gtcaagcaaa
     9781 atggttcttg caagacgttg aagtattaat taatggagtg cgtgaaacac gtcgcggtaa
     9841 aaagttagaa catcaagatc gtatagatat cccagaatta cctgaagatg ctggttcttt
     9901 cttaatcatt catcaaggtg aacaatgaag ttaaatacac tccaattaga aaattatcgt
     9961 aactatgatg aggttacgtt gaaatgtcat cctgacgtga atatcctcat tggagaaaat
    10021 gcacaaggaa agacaaattt acttgaatca atttatacct tagctttagc aaaaagtcat
    10081 agaacgagta atgataagga actcatacgt tttaatgctg attatgctaa aatagaaggt
    10141 gagcttagtt atagacacgg cacgatgcca ttaacaatgt ttataactaa aaaaggtaaa
    10201 caagtcaaag tgaatcactt agagcaaagt cgtctaactc aatatattgg acacctcaat
    10261 gtggttctat ttgcgccaga agatttgaat attgtaaaag gctctcctca aataagacga
    10321 cgctttatag atatggagtt gggccaaatt tctgctgttt acttaaatga tttagctcaa
    10381 taccaacgta ttttaaagca aaagaataat tacttaaagc agttacaatt aggccaaaaa
    10441 aaggacttaa caatgttgga agtattaaat cagcagtttg ctgaatatgc aatgaaagta
    10501 actgataaac gtgcacattt tattcaagag ctagagtcgt tagctaaacc gattcatgct
    10561 ggtatcacaa atgataaaga agcgttgtcg ctgaattatt tacctagtct taaatttgat
    10621 tatgctcaaa atgaagcggc acgacttgaa gaaattatgt ctattcttag cgataatatg
    10681 caaagagaaa aagaacgagg cattagctta ttcggaccac atcgagatga tataagtttt
    10741 gatgtgaatg gcatggatgc tcaaacatat ggttctcaag gacagcaacg tacaacggct
    10801 ttgtccatta aattagctga aattgagtta atgaatatcg aagttgggga atatcccatc
    10861 ttattattag acgatgtact cagtgaatta gatgattcgc gtcaaacgca tttattaagt
    10921 acgattcagc ataaagtaca aacatttgtc actacgacat ctgtagatgg tattgatcat
    10981 gaaatcatga ataacgctaa attgtatcgt attaatcaag gtgaaattat aaagtaacag
    11041 aaagcgatgg tgactgcatt gtcagatgta aacaacacgg ataattatgg tgctgggcaa
    11101 atacaagtat tagaaggttt agaagcagta cgtaaaagac caggtatgta tataggatcg
    11161 acttcagaga gaggtttgca ccatttagtg tgggaaattg tcgataatag tatcgatgaa
    11221 gcattagctg gttatgcaaa tcaaattgaa gttgttattg aaaaagataa ctggattaaa
    11281 gtaacggata acggacgtgg tatcccagtt gatattcaag aaaaaatggg acgtccagct
    11341 gtcgaagtta ttttaactgt tttacatgct ggtggtaaat ttggcggtgg cggatacaaa
    11401 gtatctggtg gtttacatgg tgttggttca tcagttgtaa acgcattgtc acaagactta
    11461 gaagtatatg tacacagaaa tgagactata tatcatcaag catataaaaa aggtgtacct
    11521 caatttgact taaaagaagt tggcacaact gataagacag gtactgtcat tcgttttaaa
    11581 gcagatggag aaatcttcac agagacaact gtatacaact atgaaacatt acagcagcgt
    11641 attagagagc ttgctttctt aaacaaagga attcaaatca cattaagaga tgaacgtgat
    11701 gaagaaaacg ttagagaaga ctcctatcac tatgagggcg gtattaaatc gtacgttgag
    11761 ttattgaacg aaaataaaga acctattcat gatgagccaa tttatattca tcaatctaaa
    11821 gatgatattg aagtagaaat tgcgattcaa tataactcag gatatgccac aaatctttta
    11881 acttacgcaa ataacattca tacgtatgaa ggtggtacgc atgaagacgg attcaaacgt
    11941 gcattaacgc gtgtcttaaa tagttatggt ttaagtagca agattatgaa agaagaaaaa
    12001 gatagacttt ctggtgaaga tacacgtgaa ggtatgacag caattatatc tatcaaacat
    12061 ggtgatcctc aattcgaagg tcaaacgaag acaaaattag gtaattctga agtgcgtcaa
    12121 gttgtagata aattattctc agagcacttt gaacgatttt tatatgaaaa tccacaagtc
    12181 gcacgtacag tggttgaaaa aggtattatg gcggcacgtg cacgtgttgc tgcgaaaaaa
    12241 gcgcgtgaag taacacgtcg taaatcagcg ttagatgtag caagccttcc aggtaaatta
    12301 gccgattgct ctagtaaaag tcctgaagaa tgtgagattt tcttagtcga aggggactct
    12361 gccggggggt ctacaaaatc tggtcgtgac tctagaacgc aggcgatttt accattacga
    12421 ggtaagatat taaatgttga aaaagcacga ttagatagaa ttttgaataa caatgaaatt
    12481 cgtcaaatga tcacagcatt tggtacagga atcggtggcg actttgatct agcgaaagca
    12541 agatatcaca aaatcgtcat tatgactgat gccgatgtgg atggagcgca tattagaaca
    12601 ttgttattaa cattcttcta tcgatttatg agaccgttaa ttgaagcagg ctatgtgtat
    12661 attgcacagc caccgttgta taaactgaca caaggtaaac aaaagtatta tgtatacaat
    12721 gatagggaac ttgataaact taaatctgaa ttgaatccaa caccaaaatg gtctattgca
    12781 cgatacaaag gtcttggaga aatgaatgca gatcaattat gggaaacaac aatgaaccct
    12841 gagcaccgcg ctcttttaca agtaaaactt gaagatgcga ttgaagcgga ccaaacattt
    12901 gaaatgttaa tgggtgacgt tgtagaaaac cgtagacaat ttatagaaga taatgcagtt
    12961 tatgcaaact tagacttcta agcgctgtga actgaacttt tgaaggagga actcttgatg
    13021 gctgaattac ctcaatcaag aataaatgaa cgaaatatta ccagtgaaat gcgtgaatca
    13081 tttttagatt atgcgatgag tgttatcgtt gctcgtgcat tgccagatgt tcgtgacggt
    13141 ttaaaaccag tacatcgtcg tatactatat ggattaaatg aacaaggtat gacaccggat
    13201 aaatcatata aaaaatcagc acgtatcgtt ggtgacgtaa tgggtaaata tcaccctcat
    13261 ggtgactcat ctatttatga agcaatggta cgtatggctc aagatttcag ttatcgttat
    13321 ccgcttgttg atggccaagg taactttggt tcaatggatg gagatggcgc agcagcaatg
    13381 cgttatactg aagcgcgtat gactaaaatc acacttgaac tgttacgtga tattaataaa
    13441 gatacaatag attttatcga taactatgat ggtaatgaaa gagagccgtc agtcttacct
    13501 gctcgattcc ctaacttatt agccaatggt gcatcaggta tcgcggtagg tatggcaacg
    13561 aatattccac cacataactt aacagaatta atcaatggtg tacttagctt aagtaagaac
    13621 cctgatattt caattgctga gttaatggag gatattgaag gtcctgattt cccaactgct
    13681 ggacttattt taggtaagag tggtattaga cgtgcatatg aaacaggtcg tggttcaatt
    13741 caaatgcgtt ctcgtgcagt tattgaagaa cgtggaggcg gacgtcaacg tattgttgtc
    13801 actgaaattc ctttccaagt gaataaggct cgtatgattg aaaaaattgc agagctcgtt
    13861 cgtgacaaga aaattgacgg tatcactgat ttacgtgatg aaacaagttt acgtactggt
    13921 gtgcgtgtcg ttattgatgt gcgtaaggat gcaaatgcta gtgtcatttt aaataactta
    13981 tacaaacaaa cacctcttca aacatcattt ggtgtgaata tgattgcact tgtaaatggt
    14041 agaccgaagc ttattaattt aaaagaagcg ttggtacatt atttagagca tcaaaagaca
    14101 gttgttagaa gacgtacgca atacaactta cgtaaagcta aagatcgtgc ccacatttta
    14161 gaaggattac gtatcgcact tgaccatatc gatgaaatta tttcaacgat tcgtgagtca
    14221 gatacagata aagttgcaat ggaaagcttg caacaacgct tcaaactttc tgaaaaacaa
    14281 gctcaagcta ttttagacat gcgtttaaga cgtctaacag gtttagagag agacaaaatt
    14341 gaagctgaat ataatgagtt attaaattat attagtgaat tagaagcaat cttagctgat
    14401 gaagaagtgt tattacagtt agttagagat gaattgactg aaattagaga tcgtttcggt
    14461 gatgatcgtc gtacagaaat tcaattaggt ggatttgaaa acttagagga cgaagactta
    14521 attccagaag aacaaatagt aattacacta agccataata actacattaa acgtttgccg
    14581 gtatctacat atcgtgctca aaaccgtggt ggtcgtggtg ttcaaggtat gaatacattg
    14641 gaagaagatt ttgtcagtca attggtaact ttaagtacac atgaccatgt attgttcttt
    14701 actaacaaag gtcgtgtata caaacttaaa ggttacgaag tgcctgagtt atcaagacag
    14761 tctaaaggta ttcctgtagt gaatgctatt gaacttgaaa atgatgaagt cattagtaca
    14821 atgattgctg ttaaagacct tgaaagtgaa gacaacttct tagtgtttgc aactaaacgt
    14881 ggtgtcgtta aacgttcagc attaagtaac ttctcaagaa taaatagaaa tggtaagatt
    14941 gcgatttcgt tcagagaaga tgatgagtta attgcagttc gcttaacaag tggtcaagaa
    15001 gatatcttga ttggtacatc acatgcatca ttaattcgat tccctgaatc aacattacgt
    15061 cctttaggcc gtacagcaac gggtgtgaaa ggtattacac ttcgtgaagg tgacgaagtt
    15121 gtagggcttg atgtagctca tgcaaacagt gttgatgaag tattagtagt tactgaaaat
    15181 ggttatggta aacgtacgcc agttaatgac tatcgcttat caaatcgtgg tggtaaaggt
    15241 attaaaacag ctacgattac tgagcgtaat ggtaatgttg tatgtatcac tacagtaact
    15301 ggtgaagaag atttaatgat tgttactaat gcaggtgtca ttattcgact agatgttgca
    15361 gatatttctc aaaatggtcg tgcagcacaa ggtgttcgct taattcgctt aggtgatgat
    15421 caatttgttt caacggttgc taaagtaaaa gaagatgcag aagatgaaac gaatgaagat
    15481 gagcaatcta cttcaactgt atctgaagat ggtactgaac aacaacgtga agcggttgta
    15541 aatgatgaaa caccaggaaa tgcaattcat actgaagtga ttgattcaga agaaaatgat
    15601 gaagatggac gtattgaagt aagacaagat ttcatggatc gtgttgaaga agatatacaa
    15661 caatcatcag atgaagaata ataaaaaata agacttccct atatgtaggg gagtcttatt
    15721 tttatgctag aaagtaatgc tttactatat tcaatgatta gtaatgacta actttctaat
    15781 tgtttcattg cgtaaggtat ttcattgata agtcttgatg gtggcaccac atacatatct
    15841 tttgcaaggt tttcgccaat aaaactatgt gtatatgtgg cactcataac cgcttctttt
    15901 aagttatcaa attgaccgac aaagcttgta atcataccag caagtgtatc gcccatacca
    15961 ccagtcgcca ttgctgggct accgattgtc aatttaaagt cttcatcttt aaagaaaatt
    16021 tcagtaccat gttttttaag tacaacagtt gcacctaaac gatcaactgc ttcacgatta
    16081 cgctcatatg tctgttcctc aataggaata ccacttaatc gttcccattc tttgaggtgt
    16141 ggtgtaaaga tcacacgaca tgtaggtaat tgcggtttca gtttactaaa gattgtaatc
    16201 gcatcgccgt ctacgattaa attttgatgc ggttgtatat tttgtagtag gaatgtaatg
    16261 gcattatttc ctttgaaatc aacgccaaga cctggaccaa ttagtatact gtcagtcatt
    16321 tcaatcattt tcgtcaacat tttcgtatca ttaatatcaa taaccatcgc ttctgggcaa
    16381 cgagaatgta atgctgaatg atttgttgga tgtgtagcta cagtgattaa accactaccg
    16441 ctaaatacac atgcacgagc cgctaacata atggcaccac ctaagttagc agatccacca
    16501 attaataaaa ttttgccata atcaccttta tgtgaatctt ctttacgctt aggaatgtta
    16561 atagaattta acgtttccat agtgatataa cctcccatgt aaaagccttt tccgaattta
    16621 ttcaatttta aaaatatata gtaactttta acaaaatgta ttataaattt ctgaattcat
    16681 tattatttgt cgttaaatac aatagaaaat actatacctg tatatgcaat tcgtcaatag
    16741 ataaattatt aaatatgctt acaacaatct taatatcctt taacgcacta caatagtgct
    16801 ctgataatag gttataaatg tacgtaaaac cattgtttca ataaaaatga aaacgtatac
    16861 ttcaagaagg atgggttact taatataaac aagggggtaa catatatgac tttatattta
    16921 gatggtgaaa cactaacaat tgaggatatt aaatcatttt tacaacaaca atcaaagatt
    16981 gaaattattg atgatgcgtt agaacgtgtc aaaaaaagta gagcggtagt tgaacgtatt
    17041 attgaaaatg aggaaacggt ttacggtatt actacaggtt ttgggttatt tagtgatgta
    17101 cgtatagacc cgacgcaata taatgaatta caagtgaatc tgatacgctc acatgcctgt
    17161 ggactaggtg agccattttc aaaagaagta gcattagtca tgatgatttt acgattgaat
    17221 acattattaa aaggtcattc aggtgccact ttagaattag tgagacaatt acaatttttt
    17281 ataaatgaac gtattatacc gataatccca cagcaaggct ctctcggtgc atcaggagat
    17341 ttagcgccat tatcacattt agcattagca ttaattggtg aagggaaagt attgtacaga
    17401 ggggaagaaa aggatagtga cgatgtatta agagaattaa atagacaacc tttgaacctt
    17461 caggctaaag aaggtttagc attgattaat ggtacgcaag ctatgacagc tcaaggtgtc
    17521 attagttata tagaagcaga agatttaggt taccaatctg aatggattgc tgcattaacg
    17581 catcagtctc ttaatggcat tatagatgca tatcgacatg atgtgcacgc agttcgtaat
    17641 tttcaagaac agattaatgt ggcagcgcgt atgcgtgatt ggttagaagg atcaacatta
    17701 acgacgcgac aatcagaaat acgtgtacaa gatgcatata cgttgcgttg tataccacaa
    17761 atccatggcg cgagttttca agtattcaat tatgttaaac agcaattaga atttgaaatg
    17821 aatgcggcta atgataatcc acttatattt gaggaagcaa atgaaacgtt tgttatttca
    17881 ggtggtaact tccatggaca acctattgct tttgcattag atcatcttaa attaggtgta
    17941 agtgaattag caaacgtatc ggaacgtcgt ctagagcgac tagtaaatcc tcaattaaat
    18001 ggtgatttac cagcatttct tagtccagag ccaggattgc aaagtggcgc gatgattatg
    18061 caatatgctg ctgcaagtct cgtttctgaa aataaaactt tagcgcatcc agcgagtgtt
    18121 gattctatca cttcatctgc gaaccaagaa gatcacgtat ctatgggaac tacagctgct
    18181 agacatggtt atcaaattat tgaaaatgca agacgtgtgc tggcaatcga atgtgttatt
    18241 gcattacaag cagcagagtt aaaaggtgtt gaaggattat caccaaaaac acgtcgcaag
    18301 tatgatgagt ttcgaagtat cgtgccatcc attacacatg atcgtcaatt tcataaagat
    18361 attgaagcgg ttgcacagta tttaaagcaa tcaatttatc aaacgactgc atgtcactaa
    18421 atcgacatga gtgatgtttg aaatcgttac ttgaaaaagc aaatatagtt tgctatatta
    18481 aaaataactt aataagacat tgttcgtagg acaagtaata tatagtgttc gatatcagag
    18541 agcttgtggt tagtgtgaac aagaatcaac atatatatga atctacctac ttaatttaaa
    18601 agaacaatcg gtgataaccg ttattttagt gaagtgcaat ttaggtttag tgtatcttta
    18661 taacttaaat tgttaaatag ggtggcaacg cgtagaccac gtcccttgta gggatgtggt
    18721 ctttttttat tttctaaaaa ataaagacgt acgtcattcc ataataaatg ataattattt
    18781 gggaaaggat gaaggttaat gttagacatt agattattca gaaatgagcc tgacacagtt
    18841 aagagcaaaa ttgaattacg tggagatgat ccaaaagttg tagatgaaat tttagaattg
    18901 gatgagcaac gacgtaaatt aattagtgca acagaagaaa tgaaagcacg tcgtaataaa
    18961 gtaagcgaag aaatcgcatt aaaaaaacgt aataaagaaa atgctgatga tgtgattgct
    19021 gaaatgcgca cattaggtga cgatattaaa gaaaaagata gtcaattaaa tgaaattgat
    19081 aataaaatga caggtatcct ttgtcgtatt ccaaatttaa taagtgatga tgtacctcaa
    19141 ggtgaatctg atgaagataa cgttgaagtt aaaaagtggg gtacaccacg tgagttttca
    19201 tttgaaccca aagcacattg ggatattgta gaagaattga aaatggctga ttttgatcgt
    19261 gcagcaaaag tttcaggtgc gcgttttgta tatttaacaa atgaaggtgc gcaattagag
    19321 cgtgctttaa tgaactatat gattacaaaa catacaacac aacatggtta tacagaaatg
    19381 atggtaccac agcttgtgaa cgcagataca atgtatggta caggtcaatt acctaaattt
    19441 gaagaagatt tatttaaagt agaaaaagaa ggattatata caattccaac tgctgaagta
    19501 ccattaacga atttctaccg taatgaaatt attcaaccag gtgtacttcc tgaaaaattc
    19561 actggtcaat ctgcatgttt ccgtagtgaa gcaggatcag caggtagaga tacaagagga
    19621 ttaattcgtt tacatcaatt cgataaagtg gaaatggtac gttttgaaca acctgaagat
    19681 tcatggaatg ctttagaaga aatgacaaca aacgcagaag caattctaga agagttaggt
    19741 ttaccatacc gtcgtgttat tttatgtaca ggtgatattg gatttagtgc aagcaaaaca
    19801 tatgatttag aagtttggtt accaagctac aatgattata aagaaattag ttcatgctca
    19861 aactgtacgg atttccaagc gcgtcgtgct aacatccgct tcaagcgtga caaagcagct
    19921 aaaccagaat tagcacatac attaaatggt agtggtttag cagttggacg tacatttgct
    19981 gctattgttg aaaattacca aaatgaagat ggaacagtaa caattccaga agcattagta
    20041 ccatttatgg gtggtaaaac acaaatttca aaaccagtta aataaaggct ttagctacaa
    20101 gctttaaaaa gtatatatct acgtatactt aaagcaaggg caagatactt taaataatat
    20161 tttaaaaagt ggtgacgaag ctgtcgccac tttttttgtg ctgtaaaagt ataatagtga
    20221 gccaatgcac ataacaacaa taaattaagt ttgtggttta atggggtgaa cgcatttcat
    20281 tatagcaaca atacgggata attatgatga actaaaacaa tctaaaacgt aacaagtttg
    20341 agcatcacca atataggaaa ggaagcgata aaatactgat ttcgttgata tgtagtatga
    20401 gttatatcga tggagtaggg tagggggagg ggatgattat aagggagtgg tacatgaatc
    20461 aatatcccag actcatcatc agatataaaa atttataaaa ttgatactta aaaacaacta
    20521 caaatccata gaaaatatgg aggtagtctt aaataaaaaa ttgaaaattc tcaaaaataa
    20581 aaagttaata tgaagctgac taaagactcc ggtatgtcta acctcagaca aactgatgtc
    20641 taatgttatt gcttagggta tagaactgta ttagactagg tatattattt tttcgtaatt
    20701 atataaatat aaagtggcaa aggaggtaat tgagatgaca acacatttaa gttttagaca
    20761 aggcgtgcaa gagtgtatcc caacattatt gggttatgcc ggtgttggta tttcatttgg
    20821 tattgtggct tcgtctcaaa actttagtat tttagaaatt gtcttgttat gtcttgttat
    20881 atatgccggt gctgcgcaat ttattatgtg cgcgttgttt atagcaggta cacctatatc
    20941 agcgattgta ctaactgtat ttatcgtaaa ttcaagaatg ttccttttaa gtatgtcgct
    21001 tgcaccaaac ttcaagacat atgggttttg gaaccgtgtt ggattaggtt cattagtaac
    21061 tgacgaaacg tttggcgtcg ccattacacc ttatttaaaa ggagaagcta tcaatgatcg
    21121 ttggatgcat ggtcttaaca tcacagcata tttattttgg gcaatttcat gtgtagctgg
    21181 ggctttattt ggcgaatata tctcaaatcc gcaaacgcta gggttagatt ttgctatcac
    21241 ggctatgttt atctttttgg ccattgcgca atttgaatca attactaaat cgcgattaag
    21301 aatttacata gtactcatta ttgccgtcat agtaatgatg ttatcgctaa gtatgtttat
    21361 gccttcatat ctagcaatat taattgcagc cacaatttca gcagcgttag gagtgatgat
    21421 ggaacaatga taactcatat gaacatgtta atacttattt tattgtgtgg tatcgtaacg
    21481 ctattaattc gaattatacc ttttatcatg atttcaaaag tgcaattgcc tgatgtcgtg
    21541 gttcgatggc tatcatttat cccaatcaca ctatttacgg cacttgtcat tgacagcatt
    21601 attcaacaga cgcctcatgg tgaggggtat acattaaaca tcccttacat tatcgcgctc
    21661 attccgacgg ttattttatc tataatcacg cgtagtttaa ctattacaat tattagtggg
    21721 attgttatca tggcagcatt acgatttttc ttttaaaaat aactgaaaat cattgaacag
    21781 atatttaaac ttaggtaaac tgttagtaat cagaaaattc tgatatacaa atcgtctatg
    21841 ttaagattgg gaatcgtgta tacgtaagcg atttggctaa cgcatgacaa tgataacaac
    21901 attttaaatg ataaaagtaa ttcatcactg aatctcaact aacacataac aatttcatat
    21961 ttcttattgt gagaagttga gggacttggc cctgtgatac ttcagcaacc gactttatag
    22021 cacggtgcta aaaccaacga gttactcgaa tgataagtat aaagacttct tacttttcaa
    22081 tagggtgaga agtttttttg tttaaggagg aaagaacaat gacaaattac acagtagata
    22141 ctttaaattt aggggaattt attacagaat ctggggaagt catagataac ttgcgtttga
    22201 gatatgagca tgtcggttat catggacaac cattagttgt agtttgtcat gcattaactg
    22261 gcaatcattt aacatatgga acagatgatt atccgggttg gtggcgagaa attattgatg
    22321 ggggatatat acccattcac gattatcaat ttttaacatt tgatgttatt ggtagtcctt
    22381 tcggttcaag ttcaccttta aacgaccctc attttcctaa aaaattaaca ttaagagata
    22441 tagtcagagc gaatgaacga ggtatacaag cccttggtta tgataagatt aatattttaa
    22501 tagggggaag tcttggaggt atgcaagcaa tggaactact ctacaatcaa cagtttgaag
    22561 tagataaagc cattatcctt gctgcaacaa gtcgaacatc atcttatagt agagctttca
    22621 atgaaattgc aaggcaagcc attcatcttg gtggtaagga aggtctaagt attgcacgcc
    22681 aattaggttt tttgacatat cgatcatcaa aaagttatga tgaacgtttc acgccggatg
    22741 aagtagtcgc ataccaacaa catcaaggta ataaatttaa agaacatttt gatttgaatt
    22801 gttatctgac actgctagat gtattggata gtcacaacat tgaccgaggt cgcacagacg
    22861 taacgcatgt ttttaaaaat ttagaaacaa aagtgttaac gatggggttc atagatgatt
    22921 tgctatatcc ggatgatcaa gttcgtgcat taggtgaacg ttttaaatat catcgtcatt
    22981 tcttcgtgcc tgataatgtt gggcatgatg gattcctact aaactttagt acctgggcac
    23041 ctaacttata tcatttctta aatttaaagc attttaagcg taagtaataa tatatatttt
    23101 caaagaatga agccactaaa tcacagttat catattcaaa aacatctaga gtacatgaga
    23161 gtggaaatgt attgtaaatc taaatcaagc ttgattgcta tgtagtggga ttaaataatc
    23221 atgttgaaaa tgaaagcaca caaaagcgat tcaaatgagt atgttaatta cttataaaac
    23281 atgaaagtta ttattcaatt aaatgaaata gaagcataca atctataaat tgttaatttt
    23341 cattaaagag gttaaaataa tagctatagt taaaaatatg ggagtggctt atgtgttttc
    23401 aaaaatacaa cctaaagcaa caataattgc aacgattgcg ttggtatttg tcgctttagc
    23461 tttatatcta gtgcctggtt taggactaat atttgcatta tttgcaacca taccaggtat
    23521 cgttttatgg aataaatcaa tacaatcttt cgggattagt gcacttatta cagtaattat
    23581 aacaactgtt ttaggtaata ctttcgtttt aagtgccatc atattagtct taattgcaag
    23641 tttaattatt ggtcaattgc tcaaagaaag aacgtctaaa gaaagaatat tatacgtaac
    23701 aacagtagcg atgagcttaa tttcattaat cgcttttatg ttactacaaa cattcggaag
    23761 gattccacca tcagcgagca tagtaaaacc tttcaagcaa acattacatg aagcgattac
    23821 gatgagcggt gccgatgcga atatgaccca aatattagaa gaagggttta gacaagcgac
    23881 cgttcaatta ccaggtttca tcattatcat tacattttta atcgtcttaa ttaacttaat
    23941 cgttacattt ccgattttac gaaaatttaa aatcgctaca cctgtattta agccactttt
    24001 cgcgtggcaa atgagcggta ttttattatg gatatacatt attgttatca tatgtttatt
    24061 atttacaggt caaccgagtg tgttccagag cattctttta aacttccaac ttgtgttatc
    24121 attagtaatg tatattcaag gtttaagtgt tattcatttc tttggtaaag cgaaaggttt
    24181 gccgaatgca gtaacgattt tactattggt tatcggtaca atactgacac ctacgacaca
    24241 tattgtagga ctacttggtg ttatcgattt aagtttgaat ttgaagcgaa tcatgaaaaa
    24301 taattctaaa aagtgaatag aggtggaata atgaatcggc agtccactaa gaaagcttta
    24361 ctaataccat ttgtcatcat gatcatcaca gcaattgttt taatgggtgt atggtttatc
    24421 tttaatagtc ttatagcact aattgcatct atcgttcttg tcgtgatgat tattgttagc
    24481 atcattttat tcagacaagc tttaatgaaa atggatagtt atgtagatgg tttgagtgct
    24541 caaatttcaa caacaaataa taaagcaatc aaacatttac caattggtat cattgtttta
    24601 gatgaaaatg atcacatcga atgggttaac caatttatga cagatcatat ggaagcgaat
    24661 gtcatttctg aatctgtaaa tgaagtattt ccaaacattt taaagcaatt agatagagtg
    24721 aaatccgttg aaatagaata taatcagtat catttccaag tacgttattc tgagaatgat
    24781 cactgcctct atttctttga tataactgaa caagtacaaa caaatgaact atatgaaaat
    24841 tctaaaccaa tcattgcgac attattttta gataactacg atgagattac gcaaaatatg
    24901 aatgatacgc agcgttcgga aatcaactca atggtaacgc gtgtcattag tcgatgggca
    24961 actgagtata atatattttt caaaagatac agttccgatc aattcgtagc ctatttaaat
    25021 caaaaaatat tagctgactt agaagaatct aaatttgata tcttgagtca attacgtgaa
    25081 aaaagtgttg gttatcgtgc ccaattaaca ttaagtatcg gtgttggtga aggtactgaa
    25141 aatttaatcg acttaggtga attatcacaa tcaggcctag acttagcatt aggacgcggt
    25201 ggcgaccaag ttgcaattaa aagtattaat ggtaatgtgc gtttctatgg cggtaagact
    25261 gacccgatgg agaaacgtac tcgtgtaaga gcacgtgtga tctcacatgc gttaaaagat
    25321 atccttgcag agggtgacaa agtcattatc atgggacata aacgtcctga cttagatgca
    25381 attggtgcag caatcggtgt gtctagattt gcaatgatga ataatttaga agcatacatc
    25441 gtattaaatg agactgacat tgatccaaca ttacgacgcg tgatgaacga aatagataaa
    25501 aagccagagt taagagagcg atttattaca tcagatgatg cttgggatat gatgacatct
    25561 aagacaaccg tagtgattgt tgatacgcat aaaccggaac tggttttaga tgaaaatgtc
    25621 ttaaataaag caaaccgtaa agttgttatc gatcatcata gacgtggtga aagcttcatc
    25681 tctaatccat tgttgatata tatggaacca tacgcaagtt cgacagctga attggtaaca
    25741 gagttactgg aatatcaacc aacagaacaa cgtttaacac gtcttgaatc aacagtgatg
    25801 tatgcaggta ttattgtaga tacaagaaac tttacattac gaacaggatc aagaacattc
    25861 gatgcagcga gttatttacg tgcacatggt gcagatacga ttttaacgca acatttctta
    25921 aaagatgatg tggatactta cattaatcga tctgaattaa ttcgaactgt aaaagttgaa
    25981 gataatggca tagccattgc gcatggttca gacgataaaa tttatcatcc agtaacagtt
    26041 gcacaagcag cagatgaact gttaagttta gaaggtattg aagcatcata tgttgttgcg
    26101 agacgtgaag ataatctgat tggtatatct gcgcgttcac tcggttcagt aaatgtccag
    26161 ttaacaatgg aagcacttgg tggcggtgga catttaacca atgcggcaac acaacttaaa
    26221 ggtgtgacag tcgaagaggc gatagcacaa ttacaacaag caattacaga acaattaagt
    26281 aggagtgaag atgcatgaaa gtaattttta cacaagatgt taaaggtaaa ggtaaaaaag
    26341 gtgaagttaa agaagtacca gtaggttatg caaataactt cttattgaaa aagaattatg
    26401 ctgtagaagc aacaccaggt aaccttaaac aattagagtt acagaaaaaa cgtgcaaaac
    26461 aagaacgcca acaagaaatt gaagatgcta aagcattaaa agaaacgtta tcaaacattg
    26521 aagttgaagt atcagcaaaa actggtgaag gtggtaaatt gtttgggtca gtaagtacaa
    26581 aacaaattgc cgaagcacta aaagcacaac atgatattaa aattgataaa cgtaaaatgg
    26641 atttaccaaa tggaattcat tccctaggat atacgaatgt acctgttaaa ttagataaag
    26701 aagttgaagg tacaattcgc gtacacacag ttgaacaata aagttggatt gaaataagag
    26761 gtgtaaccat tcatggatag aatgtatgag caaaatcaaa tgccgcataa caatgaagct
    26821 gaacagtctg tcttaggttc aattattata gatccagaat tgattaatac tactcaggaa
    26881 gttttgcttc ctgagtcgtt ttataggggt gcccatcaac atattttccg tgcaatgatg
    26941 cacttaaatg aagataataa agaaattgat gttgtaacat tgatggatca attatcgacg
    27001 gaaggtacgt tgaatgaagc gggtggcccg caatatcttg cagagttatc tacaaatgta
    27061 ccaacgacgc gaaatgttca gtattatact gatatcgttt ctaagcatgc attaaaacgt
    27121 agattgattc aaactgcaga tagtattgcc aatgatggat ataatgatga acttgaacta
    27181 gatgcgattt taagtgatgc agaacgtcga attttagagc tatcatcttc tcgtgaaagc
    27241 gatggcttta aagacattcg agacgtctta ggacaagtgt atgaaacagc tgaagagctt
    27301 gatcaaaata gtggtcaaac accaggtata cctacaggat atcgagattt agaccaaatg
    27361 acagcagggt tcaaccgaaa tgatttaatt atccttgcag cgcgtccatc tgtaggtaag
    27421 actgcgttcg cacttaatat tgcacaaaaa gttgcaacgc atgaagatat gtatacagtt
    27481 ggtattttct cgctagagat gggtgctgat cagttagcca cacgtatgat ttgtagttct
    27541 ggaaatgttg actcaaaccg cttaagaacg ggtactatga ctgaggaaga ttggagtcgt
    27601 tttactatag cggtaggtaa attatcacgt acgaagattt ttattgatga tacaccgggt
    27661 attcgaatta atgatttacg ttctaaatgt cgtcgattaa agcaagaaca tggcttagac
    27721 atgattgtga ttgactactt acagttgatt caaggtagtg gttcacgtgc gtccgataac
    27781 agacaacagg aagtttctga aatctctcgt acattaaaag cattagcccg tgaattagaa
    27841 tgtccagtta tcgcattaag tcagttatct cgtggtgttg aacaacgaca agataaacgt
    27901 ccaatgatga gtgatattcg tgaatctggt tcgattgagc aagatgccga tatcgttgca
    27961 ttcttatacc gtgatgatta ctataaccgt ggcggcgatg aagatgatga cgatgatggt
    28021 ggtttcgagc cacaaacgaa tgatgaaaac ggtgaaattg aaattatcat tgctaagcaa
    28081 cgtaacggtc caacaggcac agttaagtta cattttatga aacaatataa taaatttacc
    28141 gatatcgatt atgcacatgc agatatgatg taaaaaaagt ttttccgtat aataatcatt
    28201 aagatgataa aattgtacgg tttttatttt gttctgaacg ggttgatata tgttaagttt
    28261 gtgtattgaa agtgataaat tagtactgtc aacgcctctg ttaaagggtt tttaggacgt
    28321 tgaaaacgat ttgttaaaat gatttttctt ttaaaaaggc cgaaaatcaa tgttcgattt
    28381 ttatttgcat tatggtctcg atattggtag aatatcaaat ggttaaatga gaaaaacttg
    28441 gaggtgctca catgtcatca atcgtagtag ttgggacaca atggggagac gaaggaaaag
    28501 gaaaaataac ggatttcttg gcagaacagt cagatgttat cgcgcgtttt tcaggtggta
    28561 ataatgcagg ccataccatt caatttggcg gagaaacata taaattacat ttagtaccat
    28621 ctggtatctt ttacaaagac aaattagcgg taatcggtaa cggagtcgtt gttgatccag
    28681 ttgcactatt gaaagaatta gacggattaa atgaacgtgg cattcctaca agtaatttac
    28741 gtatatctaa tcgtgcgcaa gtgattttac catatcactt agcacaagat gaatatgaag
    28801 aacgtttacg tggtgacaat aagattggta caactaaaaa aggtatcggt ccagcatatg
    28861 tagacaaagt tcaacgtatc ggtattcgta tggcagattt acttgaaaaa gaaacattcg
    28921 aaagattatt aaaatcaaac attgaatata aacaagcata tttcaaaggt atgtttaacg
    28981 aaacatgtcc atcatttgat gatatctttg aagaatatta tgcagcaggt caacgtctaa
    29041 aagaatttgt aacagacaca tcaaaaatct tagacgatgc atttgtagca gatgaaaagg
    29101 tacttttcga aggtgcgcaa ggtgtaatgt tagatatcga ccatggtaca tatccattcg
    29161 ttacatcaag taatccaatt gcaggtaacg ttactgttgg tacaggtgta ggtcctacat
    29221 tcgtttcaaa ggtaattggt gtatgtaaag cttatacatc acgtgttggt gatggtccat
    29281 tccctactga attattcgat gaagatggac atcatattag agaggttggt cgtgaatacg
    29341 gtacaacaac aggacgtcca cgtcgtgtag gttggtttga ttcagttgta ttacgtcact
    29401 ctcgtcgtgt aagtggtatt acagatttat ctattaactc aatcgatgtt ttaacaggcc
    29461 tagacacagt gaaaatctgt acagcttatg aattagacgg taaagaaatt actgagtacc
    29521 cagcaaactt agatcaatta aaacgttgta aaccaatctt tgaagagtta ccaggttgga
    29581 cagaagacgt aacaaatgtg cgtactttag aagaattacc tgaaaatgca cgtaaatatt
    29641 tagagcgtat ttcagaatta tgtaatgtac aaatttctat cttctcagtt ggtccagata
    29701 gagaacaaac aaacctatta aaagaattgt ggtagaactt tatataagtc atacacaatg
    29761 attataaata catgagcctt ctatctttat tggtaggagg cttttgttat gcttgcttct
    29821 gtatcgattc gattatttag ataaaaaata ctaacgtaaa ggcgatattt gctagtcata
    29881 atttagaaga ttagatgata tttaacgaaa attaagatga aatacttgaa tgtaagaagt
    29941 ctgatgtcga aaatagctat taaaatagag tagacgtaag tgtaaatgaa agcacctaaa
    30001 atagaaaaat ttcaaaaata gcgtaattat tataatgaat agactgccaa taaaatgcaa
    30061 tttttcactt ataacattct tcaaaaaata atagcaaaat tatgtaaaaa atatcttgtc
    30121 atggcaagat tggctgtgct ataatctatc ttgtgcttaa gaa
//
LOCUS       DALAAR010000031        30126 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000031 DALAAR010000000
VERSION     DALAAR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.031"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            41..1441
                     /locus_tag="O2458_001389"
     CDS             41..1441
                     /locus_tag="O2458_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887256.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            1667..2602
                     /locus_tag="O2458_001390"
     CDS             1667..2602
                     /locus_tag="O2458_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCZ3887257.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            2924..3775
                     /locus_tag="O2458_001391"
     CDS             2924..3775
                     /locus_tag="O2458_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ3887258.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(3867..5363)
                     /locus_tag="O2458_001392"
     CDS             complement(3867..5363)
                     /locus_tag="O2458_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCZ3887259.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            5595..7424
                     /locus_tag="O2458_001393"
     CDS             5595..7424
                     /locus_tag="O2458_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ3887260.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(7564..9642)
                     /locus_tag="O2458_001394"
     CDS             complement(7564..9642)
                     /locus_tag="O2458_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ3887261.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(9780..11189)
                     /locus_tag="O2458_001395"
     CDS             complement(9780..11189)
                     /locus_tag="O2458_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3887262.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            11475..12281
                     /locus_tag="O2458_001396"
     CDS             11475..12281
                     /locus_tag="O2458_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ3887263.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(12344..13000)
                     /locus_tag="O2458_001397"
     CDS             complement(12344..13000)
                     /locus_tag="O2458_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCZ3887264.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            13113..13586
                     /locus_tag="O2458_001398"
     CDS             13113..13586
                     /locus_tag="O2458_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ3887265.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(13709..14920)
                     /locus_tag="O2458_001399"
     CDS             complement(13709..14920)
                     /locus_tag="O2458_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ3887266.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(15102..15761)
                     /locus_tag="O2458_001400"
     CDS             complement(15102..15761)
                     /locus_tag="O2458_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887267.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(15821..16963)
                     /locus_tag="O2458_001401"
     CDS             complement(15821..16963)
                     /locus_tag="O2458_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ3887268.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            17231..17617
                     /gene="gtxA"
                     /locus_tag="O2458_001402"
     CDS             17231..17617
                     /gene="gtxA"
                     /locus_tag="O2458_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCZ3887269.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            17751..17858
                     /locus_tag="O2458_001403"
     CDS             17751..17858
                     /locus_tag="O2458_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ3887270.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
     gene            18486..20249
                     /locus_tag="O2458_001404"
     CDS             18486..20249
                     /locus_tag="O2458_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887271.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            20274..22007
                     /locus_tag="O2458_001405"
     CDS             20274..22007
                     /locus_tag="O2458_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887272.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            22238..22405
                     /locus_tag="O2458_001406"
     CDS             22238..22405
                     /locus_tag="O2458_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887273.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            22459..23145
                     /gene="bioD"
                     /locus_tag="O2458_001407"
     CDS             22459..23145
                     /gene="bioD"
                     /locus_tag="O2458_001407"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCZ3887274.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            23123..24481
                     /gene="bioA"
                     /locus_tag="O2458_001408"
     CDS             23123..24481
                     /gene="bioA"
                     /locus_tag="O2458_001408"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCZ3887275.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            24483..25490
                     /gene="bioB"
                     /locus_tag="O2458_001409"
     CDS             24483..25490
                     /gene="bioB"
                     /locus_tag="O2458_001409"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCZ3887276.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            25468..26583
                     /locus_tag="O2458_001410"
     CDS             25468..26583
                     /locus_tag="O2458_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCZ3887277.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            26594..27286
                     /locus_tag="O2458_001411"
     CDS             26594..27286
                     /locus_tag="O2458_001411"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCZ3887278.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            27297..27764
                     /locus_tag="O2458_001412"
     CDS             27297..27764
                     /locus_tag="O2458_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCZ3887279.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(27816..28793)
                     /gene="hlgB"
                     /locus_tag="O2458_001413"
     CDS             complement(27816..28793)
                     /gene="hlgB"
                     /locus_tag="O2458_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCZ3887280.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(28795..29742)
                     /gene="hlgC"
                     /locus_tag="O2458_001414"
     CDS             complement(28795..29742)
                     /gene="hlgC"
                     /locus_tag="O2458_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCZ3887281.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
BASE COUNT        10205 a         4326 c         5503 g        10092 t
ORIGIN      
        1 aaaatttgtt tttcttaaac ataggaggct tatctaattc atggacacat caaaacaatt
       61 tagaggtgac aaccgattgc ttttgggtat cgttttaggg gttattacct tttggctatt
      121 cgcgcagtca cttgttaatc ttgttgtccc attacaatca acatatagta gtgacgttgg
      181 aacgataaat atcgctgtta gcttatctgc cttatttgct ggtttgttta tcgtaggtgc
      241 tggtgatgtt gctgataaat ttggtcgcgt caaaattact tatgtaggat tgatattaaa
      301 tgttgtaggt tcattactca tcatcattac acctttgcca gcatttttaa ttataggtag
      361 aataattcaa ggtttgtctg cagcatgtat tatgccatca acacttgcta ttattaacga
      421 atattatatt ggtacaagaa gacaacgtgc cttaagctat tggtctattg gttcttgggg
      481 tggtagtggt atttgtacgt tgtttggtgg cttaatggct acatatatag gttggcgttc
      541 aatatttgtt gtttcaattc tattaacatt attagcaatg tacttaatca aacatgcacc
      601 tgagactaaa gcagaaccaa tcaaaggtat gaaagcagaa gctaaaaagt ttgacgttat
      661 tggtttagtc attttagtag tgacgatgtt aagtttaaat gtaatcatca cacagacgtc
      721 tcattttggt ttagtttcac cgttaattct aggtttaatt gttgtgttta tctgttcatt
      781 agttggtttt gtatattacg aaaataaaat aaagcatcca cttgttgatt tttcaatttt
      841 taaaaataga ggatacagtg gtgcaactat ttcaaacttc ttattaaatg gtgtagcagg
      901 tggtgcactt atcgttatta acacgtatta tcaacaacaa ttaggattta attcttcgca
      961 aacgggttat atttcattaa cgtatttaat aacagtgttg tcaatgattc gtgtaggtga
     1021 aaagatttta tctcaacatg gtccgaagcg cccactatta ctaggaagtg gctttacagt
     1081 gattgggtta atcttattgt cgttaacatt tttaccagaa gtgtggtata tcatatctag
     1141 tatagttgga tatttattgt ttggtactgg tttaggatta tatgctacac catcaactga
     1201 tacagcagtt gctagtgcgc cagatgataa gtcgggtgtt gcttcaggtg tgtataaaat
     1261 ggcgtcatca ttaggaaatg catttggagt agcagtatct ggtacggttt atactgtgtt
     1321 agcagctaat ttaaatttga acttaggtgg tttcacaggt atgatgttta atgccttgct
     1381 agcaattgtt gcatttttag tcattttact attagttcct aaaaatcaaa cgaatttgta
     1441 aaactgaaat gaaagcaagt tattatgtag ggattttaaa ggaaattttg tgaaagtaag
     1501 tttatcatac acacttaatg ttgcgtattg acgtttaatg ttaggtgtgt tcttttatag
     1561 acgataaaag ctgtgtgcat attaagcgaa tgattttcaa attgacgcta atatgcgaaa
     1621 gtagtatttt taaaatgaac aacaacgatg aagaggggtt tataggatga aaattgcaat
     1681 tgctggatcg ggtgcattag gtagtggctt tggtgccaaa ctatttcaag caggatatga
     1741 tgtcacactt attgacggat atacatctca tgttgaagcg gttaagcaac atggattaaa
     1801 tataacgatt aatggagagg cattcgagtt aaacattccg atgtatcatt ttaatgatca
     1861 accggacgaa agcatttacg atgttgtctt tctatttcca aagtctatgc aattaaaaga
     1921 agtgatggaa gatatgaagc cacatattga taatgaaacg atcgtcgtat gtacgatgaa
     1981 tggtctgaag catgaagaag tcattgcgca gtatgttgct caatcacaaa ttgtcagagg
     2041 tgttacgact tggacggcag gtcttgaaag ccctggacac agtcatttac ttggtagtgg
     2101 accagttgaa ataggtgaac tagtggatga aggtaaagaa aatgttataa aagttgctga
     2161 tttacttaac gaagcggaat tgaatggtgt cattagtaaa gatttatacc aatcgatttg
     2221 gaaaaagatt tgtgttaatg gtacggcaaa tgcattaagc acagtgttgg agtgtaatat
     2281 ggcatcgctg aatgaaagta gttatgcgaa gtgtttgatt tataaattaa cgcaagaaat
     2341 agtgcatgta gcgacgattg ataatgttca tttaaatgtt gatgaagtat ttgaatattt
     2401 agttgattta aatgaaaaag ttggtgcgca ttatccatcc atgtatcaag atttaattgt
     2461 taataataga aaaactgaaa ttgattatat taatggcgca gttgcaacat taggtaaaca
     2521 acgtcatatt gaagcgccag tcaatcgctt tattactgat ttaattcata ctaaagaaag
     2581 tcagcgacac gcacaggatt aattggtgtt ttaggtatga ttacaaggaa atgacaatca
     2641 cgtgatatta cggtcattat taagattgaa atgtaataaa taaagaacag cagtaaggta
     2701 ctttcaaatt gaaatgatct tggtgctgtt tttcttgatt gatcttcgtc ataattcaga
     2761 tttgtcatag gtctacgaca tactattagt atttactaga cagtttttac gacgacactt
     2821 tgaaaaattt tgaggcaaat catttggaag tctcacgtga attttgtaaa ctcatcaagc
     2881 aagtaattat attaaaaaga caaatagaga aaaggtgttt ataatgagta aaatttttgt
     2941 aactggtgca acgggcctta ttggcattaa attagttcaa agactaaaag aagaggggca
     3001 tgaggttgct ggttttacta catctgagaa tggtcaacaa aagctagctg ctgttaatgt
     3061 aaaagcatat attggtgata tattaaaagc tgatactatt gatcaagcgt tagcagattt
     3121 taaaccagaa atcattatca atcaaattac ggatttaaaa aatgttgata tggcagcaaa
     3181 tacgaaagta cgtattgaag gttctaaaaa cctaattgat gcggcgaaaa agcatgacgt
     3241 taagaaagta attgcccaaa gtattgcctt tatgtatgaa cctggcgaag gattagcaaa
     3301 tgaggaaact tcacttgatt ttaactcaac tggcgataga aaagtaacgg ttgatggtgt
     3361 ggttggttta gaagaagaaa cggctcgtat ggatgaatac gttgttttac gttttggctg
     3421 gttatatggc ccaggtactt ggtacggaaa agatggcatg atttataatc aatttatgga
     3481 tggtcaagtg acactttcag atggcgtaac atcatttgtg catcttgatg atgcagttga
     3541 aacatctatt caagctattc attttgaaaa tggtatctat aatgtagcag atgatgcacc
     3601 tgttaaaggt tctgaatttg cagaatggta taaagaacaa cttggtgttg aaccaaatat
     3661 tgatattcaa cctgcgcaac catttgaacg tggcgtaagc aatgagaagt ttaaagcgca
     3721 aggtggtact ctgatttatc aaacttggaa agatggcatg aatccaatta aataataatt
     3781 tatccgttta atatacaaag aataaagact tggtcgaatc gtggatgata tattatcaaa
     3841 cgcacggctc gaacaagtct tttttattat gtcttcgtta tctttgtatg aaggaataac
     3901 agaattacaa ttaatgtact gaataatgca attaatgttg tgattagtgc taatttaatt
     3961 tctattggta gccaagtcag tacaaaagac caattattgc taccgagaat gagatatggt
     4021 aatgcatata atatgagcgc taaagcgata catatacata atgataacca actcaataca
     4081 gcaatccgtt ttgatctacg caaaagaatt tgaccatggc gcatttgcca agcacgataa
     4141 gctgagaata taaatgctaa taaaataagt gttgtcatca aaacggtaac aatattaaat
     4201 tgatctttat attgattgag catagacgca accgtcgaat atcgcttgtg atttacaatt
     4261 tgtgtattaa gatgctcaac taatttgggt acatattccg aatttagatt tgcaagtaca
     4321 acaattccat aattttgttt tggatttagt aaaataaatg atgaaaagtt atctagcgtt
     4381 cctgaatgaa acactaaatg ttcatcatta ttggtaaacc agccggaagc atatgcattg
     4441 gcattaggtt caccaattgt tgaagataaa tttttatgtg attgttgaac taatgatttg
     4501 tatttatcag gtggattaag ttggaatttt atccaatgtt ccaaatcttc agttgatgtc
     4561 atcatatatg ctgatggtgt atcccaaagg ttaaattcag gtttagagac gacaggtgtc
     4621 gaaccttgta attcatagcc aatagcatca tgttttgatt tgtaattggt ttgtttgaat
     4681 gatgtatgtg tcatatgcaa aggcttgagc catgaatttg taatatattt tgtataggat
     4741 tgcttcgtaa cgttttggat aattaaacct aataaatcat agttcatatt tgagtattca
     4801 aattcttctc cgggcttatg atgtaattca tcacccataa ttgcacgggt tacatcattt
     4861 aaacgattat ttttgcttgt cacagaatct tcgcttgtaa tatcactagg tataccactt
     4921 gtttgagcca aaagttgctt aatcgtaata gtttcatttt gaccattata gttcatttta
     4981 aaatgaggca catgtttgga tacggcatca tttaagttta atcgaccttc ttgagctaat
     5041 tttaaaattg caagacctgt gaaagctttc gtattagaag caatttcata ctttgttgtg
     5101 ggagaagctt tgactttttt atcaacatct tgataaccat aacctttatt taaaaaaact
     5161 ttgccatttt ttactattaa aattgatgct ccaggaatgt gtcccttttg taaatcatgc
     5221 tcgataattg tatctatttg ttgttgcgaa tcattggtta accgtgtctt cgtattgcta
     5281 tttaatgtta tatatatagc aattgaaatg gcgactaaaa tgataataga aatggataga
     5341 aaatacagtt ttttagtagt cataaacgtt cctttttata tgttaaattt acttaagtgc
     5401 ctaatgagat ttattttatt ataccagttc tattgaataa attaattaaa gaggtatatt
     5461 taccattatt tttgacacct tatatgttaa taggtgtata attacataaa aaatattaat
     5521 taactctcta tactttttgg aaagataggc gtatagtgat atccatcaaa aactataaaa
     5581 aggaatagga gattatgttc aatcaattta aaagacttat tatagggcaa cctaaaaaaa
     5641 acagagaact aaaagacgaa aagatatcga agtttaaagg gttagcgatt ctttcgtcgg
     5701 atgcattatc ttcagtggct tacggtcccg aacaaatact gattacacta tcagtagtgg
     5761 gtgcagttgc aacttggtat actttaccga ttgcaggtgc tgttctgatc ttattagctg
     5821 ctttaattat gtcttatagg caaattattt atgcatatcc taaaggtggc ggcgcgtata
     5881 tggtgtccaa gaccaattta ggagaaaaat ggggattgct tgcaggaggc tcattattag
     5941 tcgattacat actgactgtc gctgttagta tatcatctgg tgctgatgca tttgttgctg
     6001 cattcccaag tttatatggg cataaagtac tgattgcatg tttacttgta ctttttattt
     6061 taatattaaa tttacgtggt ttaactgaat ctgccaccgt attatcctat ccagtatatc
     6121 ttttcattat agggttagtg atattaatat ttataggtac tttccgtgtg gcgacaggag
     6181 atattcaacc acatatgcat gcatcagtag gaactgcggt tcctggagta acattattct
     6241 tgctattaaa agcattttca tcaggggctt catcgttaac gggtgttgaa gctatttcaa
     6301 atgcggtaac caattttaga gagccaagtg ctaaaaatgc tgtgaaaacg ttaattgcta
     6361 tgggttcaat tttagcgttt ttattagttg gtatcgttgg tttagcatat gtttatggaa
     6421 ttttgccaca aacggaaacg acagttttat cacaattagc tatgcaaata tttggtgata
     6481 atgctgcctt ctattttgta caggcaacga ccgtgatgat tttggtatta gcagccaaca
     6541 ctggatttac agcttttccg atgttagcgg cttcaatgtc taaagataaa tatatgccta
     6601 gaatgtttac tgttagaggt gatcgcttag gctactctaa ctcgattatt attttaggtg
     6661 tattagccat tattttaatt attgtatttg atggtatgac tgaagattta attccattgt
     6721 atgcggttgg tgtatttatt ccatttacac tagcacaatt cggtatggtt attaaatgga
     6781 ttcatgaacg tcctaagaat tggttaagta agttgtctgt gaacttactt ggtggtatcg
     6841 tgacatttat tgtttttatg attttactca taactaaatt tagccaagta tggccgatac
     6901 ttattttctt gccgttcgtg gtgattttct tcttgaaaat taacaaacat tatcgagata
     6961 tcgcagaaca attacgttct gacattgatg ttcttaatgt ggacgtggta gatcgtaatt
     7021 tagcaattgt gccgataaca agtattacaa cagcagttga taagtctatt tattacgcac
     7081 aaatgcttgc gaataatgat gtcatagcag tacatgtatc atttggagat gaagatgaaa
     7141 aagcattcca agagaaatgg aaacgtcatt tcccagatgt aagattagtt attttacatt
     7201 cggaatatcg cagtattata cgaccaattt cacgttttat cgataagatt aatcgtaaag
     7261 ccaacgatca aaactatatg attacagtag tgataccaga atttattact aagaaacgtt
     7321 ggcataattt attacacaat caaactagtt tgcgtatgaa attgtattta atttatcaaa
     7381 agaatgttaa cgtatgtaca attccattta aacttaaaaa atagattcta taatcaaatc
     7441 ggattgatga aaggttattt acttttcatc aatctgattt tttgatgttg aatataaaaa
     7501 ccccgtacat atgagatgat aatgactttg tctcatatgt acgggggtat tgttatgtct
     7561 tgcttaatgt ttggactgta aagacaagac aatttcgttg taattgatat tttctcgaat
     7621 ttgagctgca acgatattgt tgattttatt tttgcgaatt aattcatcta aaagatgacg
     7681 ttgtaagtac aatgcttcca atttgtggtt atggaacata gcttgcggta tgatttgatt
     7741 gctgttttct tgacgttctt ttcgacgttg tgcacgccac tttaatgagc gactcatatc
     7801 atagtaatga ttaatgacta agctaacctc aagtacattt gtactatttt gttcttcttt
     7861 taagcgtaaa ataatattgt gattcacaat acgcataatt ttttgaacat ctttgaatga
     7921 attttcaaat tcttgcttct tagattcttt atttaaggct ttgtagtgct tcatcgcttt
     7981 aagtctaagc cataacatct tgaagaagtt tgcaaatttt tcaacaaatg atgcagtacg
     8041 gtgtgtctct gttaattcga caatattgcg gtaattatta atatcctgat atgttgcctt
     8101 acctttatca attaaacgct caagtgtaga tgtttcgatt acttttgcaa tatcttctaa
     8161 acgtttgagt tcttttgtat tttgattatc aggttctgaa tttaataaaa ataacaattc
     8221 tccatagtat tggtttaata ctgggcgata atttgtatcg tttttgtctt ttttactttc
     8281 gtttttaaaa tgctggatca ctttttgaac aatgaaaatt ttggcagatt gataagtcat
     8341 acctttaaaa gtagtatctt cggcagatgg tgtaattaaa ggtaaaacaa tttgcgctaa
     8401 gattaaacta attaatacca tgaaagatgc aataaacaat aaatcattac ggtattcgaa
     8461 tgcttgtcct tttgtaatga taaatggtaa tgtaagtgcc attgaaagtg aaattgtacc
     8521 gtgaatacca cacatggtca taataaatgc gtaacgcact cgagaaggtg gtgtttcatg
     8581 tgaatcatgt tcctcgtcta gataagattg tatatttttc gggaaataaa aatctttgta
     8641 ccagaaataa acccaaacaa atcgacatgc ataaattgca atagcgatta atatagtgat
     8701 cactattaaa aatgaaaagt tgtcaggctc tgtttggaaa atatctatga ctacagtagg
     8761 aatcataaaa cctaatacaa caaatacaaa accatttaag gcataactaa atgtgttcca
     8821 aatttgatgg taattcattt gtagttccgt ttgtgctcta attaaacggt cgcgttctaa
     8881 accatggatt agacctgcga ttacaactgc aatgatacct gaagcatgaa cttcttctgc
     8941 taaaaagtat acgacaaaag gagttaataa ttgaataaaa gttaaggtat tgttatcttt
     9001 taaaccttta ttagcggtta agtctatacg tattctaacg acaacgaatc caataattgc
     9061 accaataagt acacctagta ttgttgaaat gataaattgt tcaacagctt ggaataatga
     9121 aaaggtacca gttactaatg cagtaacagc aattttaaat gaaatgatac ctgctgcatc
     9181 attgagtaaa gattcacctt ctaaaatcgt catagaacct ttaggtaata attttccgcg
     9241 tgtaatagca gatactgcta ctgcatcagt aggacataaa attgctgcta ttgcaaaagc
     9301 ggctggcatt ggtaaggcag gccaaatcca atgtataaaa tagccaacac cgactacagt
     9361 tgcaaacact agtgccattg acattaatag tataggttta cgatattcta ataattttgt
     9421 tcgagagacg tgggtacctt ccacaaaaag tagtggcgcg ataacggcaa acataaatac
     9481 ttcagaattg aattggaaat caacttgtat tggaataatg aaaataacga cacctaatgc
     9541 aatttgaata aaggcagtag gaatttgtgg gaatcgatta ttgataaccg aactaataat
     9601 cacagcaaaa ataaaaatta aaaatgcttc taatagtgcc atacaatact cctcaaaatt
     9661 ttaatagtta atattttatc acttttaagg cataatgaca tagatatatt gataaaatga
     9721 agttattttc aaaaaaactc tagtatcggt tgaactgata ctagagcgag atgtttaaat
     9781 tattgattgt catatctgaa atgaccgctg tcattttgtc gttgttcata cgcgagcttt
     9841 tcagcattcg ttttgtattt tttataaaag aaaaataaaa atataaacca gaatggcgaa
     9901 atataaatag ctgctcttgt ttcgtcacta aagaataata aaatgaatac aaagaagaag
     9961 aacgctagaa taatgtaagc aataggctta ccaccaatca acttaaattt actgttttta
    10021 tgtgcctcag gatgcttttt caaatacatt agataagcga caataatcat tgcccaaaca
    10081 actaaaaaca acacagttga taacgttgta acgtatatga atagttgaat tgcattaggg
    10141 aaaatgtagt ttaacaaggc tgctataagt aatgcaattg atgaaactaa cattgataaa
    10201 taaggcacgc catgactatt cgttttattt aaaactttag gacctaaccc ttgttgtgac
    10261 agtccgaata agatacgact attcgaatag ataccactat ttgtagcaga ggccgcggca
    10321 gttagcacga caaagttaac gacacctgct gcaaatggta cgccgattaa tgtaaagagt
    10381 ttaacgaaag ggctactatt tggatcgata tcattccaag gtatgactga cataattact
    10441 aatagaccac cgatatagaa taataaaata cggataggta cattattaat tgctttcggt
    10501 aaggtttttt caggatcttt cgtttcacca gcagttacac ctataagttc aataccaatg
    10561 aatgaatata cagcaatttg gaatgacatt aagaaaccaa atgttccacc agggaacatg
    10621 ccaccgtgac tgattaagtt tgtgaatgat gcatgtccat aatgtgtttt aaatgagaaa
    10681 aagattaata caagaccaac gataaccatc gcaataattg tgacaacttt aataatcgag
    10741 aaccaaaact ccagttcacc aaataatctg gcacctaata agttgaagct aatcaagatt
    10801 aaaacaataa atagcacggt aatccaattt gggacttgtg gataccaaaa tgcaaagtat
    10861 tgtcccatcg cagttaggtc tgacatactt gatacaatcc aacataacca gtaagtccag
    10921 ccaatgacaa agccaccaaa agggcctaaa tattcatttg caatatcaac aaatgaatta
    10981 aatcttgtat tgctcaacaa caattcgcct aatgcgcgca taaaagcgaa tagtacaacc
    11041 ccaataatca tgtatgtgaa taacagtgat ggaccagtta aagatattgt ttgacctgat
    11101 cctaggaata acccagttcc aattgcgccc ccaattgcta ttaattggat gtgtctgtta
    11161 ttcaactctc tatgcaattt tcttgccata attttcctcc tcaggataaa gcaaataaaa
    11221 aagttgtgtg taagcgtttt cttaattata aatactaaag aaatccattt aataaagcaa
    11281 ttattttttt aaatataaga aaaaacattt gataggtaag ggtatgataa ctaaaactta
    11341 tcgatttttt gaaaagatat aagtttttat gcaaaaatat aaaataatca tctaaatgaa
    11401 gcagttaact atatatatta gataagctat ggcataataa ataacatttt aatttgagta
    11461 aagaaggtaa gggtatgaag atagggacaa tcgccgattt acatattgat cgtcataata
    11521 aaaaaacatc cgaagattac ttagaggcat tggttgaaat agttaagtat aagaaattgg
    11581 atatattgtt gattgcgggg gatatctcaa atcattatca attgacgcac caatttataa
    11641 cgcaattgac aaaacaatta gatatacctg tgaaatttgt acctggaaac catgatttat
    11701 gggaagttga aagtatgact acgcaagaca tttggaataa ttataagagt atgtcacagt
    11761 gcttggtagg aaaaccattt atagtaaatg aagaatgggc aatcatagga catactggct
    11821 ggtatgatta tagctttgca gcacaacgat tttcattaga tgagttacaa aaaggaaaac
    11881 attatggtgc gacttggcaa gataaagaac gaatatcttg gggcatatca gatcaaaatt
    11941 tatctaaaat agcggctgaa caagtgaaga aagatatatt agaagtagga aatagacgag
    12001 tgattttagt cacacatgtt gtgacgcacc ctgatttcat tgttcctatg ccgcatcgta
    12061 tattcgattt ttataatgca tttattggga caagtgattt caatcctttg tatgcgatgt
    12121 tcgatatacc atatagtatt atgggccatg ttcattttcg taaaagtgtg atagatgatg
    12181 gcagatgtta tctctgtccg tgtctaggct atccaagaca atggcgttca gaagatattt
    12241 accaggaaat aaatgagacg atacaaataa tagaaattta aaatgcgcaa acctgaccca
    12301 gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt tacttacgtg tgtttatttt
    12361 gttgctgatt ttcaattgta tatgaatgtg gttgcacata aatgcacttt cttcctggtg
    12421 aattaaagct gtattccatt ttctctttac gaattttaat aatttgtttg cgatttggaa
    12481 tgatggcagg taaaactagg ccacgacgaa tatgactcca aagtggatcg atataatagt
    12541 aatagtaatc atcgtaccca atgagtaacg ttacgtgaat atttgaaact tgtttcgctg
    12601 gcttgttatc aaagcggaaa acacgtagta atggtttaga accaagatta gtatggtata
    12661 ttaacacagg ttgaccttga tcgataatac ctttaagatc ttctaacgat ttaccagtgc
    12721 cgtctacgat attaggattg tatttttgta aaaatggtac atatgcttct ggaaatatcg
    12781 tttgatgata attgccaagc ttaatgaata agtgatgtcc aacataacct ttatgtggat
    12841 tgttcggatg tgtcggccaa tgtctcataa tttctgtagc agggatatgt tggttgttgt
    12901 attgcaacat catggctgcg gaaacacctt cacaccccat gaccataggg ataggaaata
    12961 gctgactgat aggtttaact ggtaatattt ttcggttcat aatatagtcc tcgcattgat
    13021 tcaataaata tttaatataa ttatatagcg tcaatgcaaa atgtcctaaa catatgtttt
    13081 acatgagtga ataaaattaa tggagtgata aaatggaata tcaattacaa caacttgcgt
    13141 cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg acgacaggct cagcaacata
    13201 tagcagggtt ttggcaaaga tgttatcaag agggagtaat tgcggattta cagttaaaaa
    13261 ataatggtga tttagccggg atacttggct tatgtatacc tgaattagac ggtaagatgt
    13321 catatatgat tgcagttacc ggagataata gtgctgatat tgaaaaatat gatgtcataa
    13381 cattagcaag ttcaaagtat atggtatttg aagcacaggg cgcagtacct aaagcagttc
    13441 aacaaaaaat ggaagaggtt catcactaca tacatcaata tcaagcagat acggtaaaat
    13501 cagcaccatt ttttgagttg tatcaggatg gtgatactac aagtgaaaaa tatattacag
    13561 aaatttggat gcctgttaaa gggtgattga atagaaatgt ccttgctatg aaggcgtaat
    13621 tttagttgaa gattcgatct cataattcat aaagaagaac cctgacaaag atagatatct
    13681 ttgctagggt tctaattatg ttgaagtgtt atgcgacatg ttgcttttta atcattttaa
    13741 agtaaatgat ttgtagactg actaatagaa tggctgtaat gaagataata atcatatatg
    13801 gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc aacagcgcca cctaagataa
    13861 attggaataa gccaagtaaa cttgatgcgt taccactgcc acctgttcgt tcttccatag
    13921 ccattgtgaa accaagcggt ccaattgacg tcacaggaca cacatttaag aagaatgcga
    13981 tgagtaagac ccaaagtggt aaatggaatg taagtgtcag gataattaaa gcaacaccta
    14041 ccacttgtat aatagttaaa atgattaata atatgtggcg atgtaatttt tctactaata
    14101 aagcaacgac ttgactgaca atgattaaac ctacaccgtt aacagcaaac atgatactaa
    14161 attgttgggg tgtcatatta tacaattttt gagtaataaa tggcgatgca gatgaataac
    14221 taaatagcat tacataagtt aaaccttgta atagcattgg aataataaat gctggttttt
    14281 tcaaaagact tccaaaatct ttaatgacgc tactaaaatt cacctgcttt aattcatctt
    14341 tagatgtttt aggtaattga gaagcgacgc caattaaaat gatgagtgcc acaatagtta
    14401 aaattgtgaa aatagaacgc catgttgcta cggataaagc taatccaccg gctaatggtg
    14461 caagaatagt gatgatgcca ttaactacca ttaaagatgc taaaaattta gcgagtgcgt
    14521 tgccactaaa tttatcacca gcagaggctt ttgcaatcac gatgacgcca ccaccagtta
    14581 aaccttgaat aaatcgtaaa gttaagaata atggcaattg atcaacaaac atagacaaac
    14641 ctgataccaa aatgaaaatg atcaaaatgg atacagcaat ccgtttgcga ccaaaagcat
    14701 cggataaagg tccaaagata aattggccta gtgcaagacc aatcattgtg aatgataatg
    14761 ttaattgaat ttctgatgta gtagaaccaa aatcaccttg cactttaggt aatgatggta
    14821 cgtacatatc tatagttagt gggccaaaag cggtcatgac gcctaacgta attagcaata
    14881 aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat gatcagactc ctttaaatga
    14941 tataattata taaattatat acgttttgtg tgtatttgta tgtacttaga atacaagttt
    15001 atataaaaaa tccctatact cttaatgcat catcacgaat aacgtagtga tacagataaa
    15061 gtatagggaa ttaatgttta tcatcaaagc aggtgtaata attaattagc gccaccattg
    15121 tcatctttaa ataagctttc taatttagat ttaatactgt cgaattttgt attaatatca
    15181 tttaacatgc tttcaatttt accttggctt gcattgtttt taagtgcatt atttgcctct
    15241 gtcaatttgt tgatttggtc ttgataaaat tgagttagct tagaatcagc tttgttttgt
    15301 tcttgctgct tcaagttgtc gatttgattt tgtaaatttt taataatgtc atcagttttt
    15361 tggttgtttt taatattttc tttagcatta tctacttgct ggttaatttg gccgtcttga
    15421 ttttcaactt gttttttata attttgagag actttagggt cattactaac tttatcgcta
    15481 tcattgttaa gtaaggcgcg tgtgacaaaa atagcgacaa taattaagag aatggcaata
    15541 atgatatata accatttttt tgaacggcgg gatttctcgt tttcttcttc aagaatttgt
    15601 tctcttcgat aatctctacc attatagtat cgttccgggt gttcttcagg atcgccttta
    15661 gcatattgtt gtctatacga tgcgtcttca gattgacgac gatgattttg gttttgattg
    15721 tcgtattgat atgaatcacg gtatttatca gttcttttca tgggtttcac ctcattcaag
    15781 ttttaactgc aaataataaa gctgatgatt tactttgaca ttattgtaat ttcagaatac
    15841 gagcaatgtc agttgcagtg tttaataaat tttgttcgct attttgtagg acatcttcta
    15901 aagtgctagg tgaagagatt atagaatagg cactatcaat accgaaatcg taaacatgtt
    15961 gataattttc gcctagactg ccacaaatcg cgatgacagg aatatgatat tgttttgcag
    16021 ctaacgcaac gcctacgggt gttttaccaa agatggtctg ataatccatg cgtccttctc
    16081 cagtaataac gaggtctgca tctttaattc tttgatgaaa atctgtaatg tcaaagacga
    16141 catcaatacc ttttgttaaa gttgtctcac aaaacgctaa taatgctgcg cccataccgc
    16201 cagctgcacc agaacctggt atttgattaa cggactttcc tgtgcacatt tttatcttat
    16261 catgataatg cgacattgcg aaatccaact ttggtatcat ctttgcatca gcgccttttt
    16321 gaggaccata aatataggta gcaccatttt cacccaataa aggatttgaa acatcacagg
    16381 ccactttaaa ggtcacctct tttaatcgcg aatctagatt ggttatatcg atttgtgcaa
    16441 tgtgagcaag attagcacca ttcatttgta ataagtcccc gtttacatca gtaaacttta
    16501 cgcctagtgc acttagcata cctgtaccac catcatttgt tgcactgcca ccaatcccta
    16561 aaataatggt cttagcacca tgatttaatg catctttaat tagttcaccg gtaccatatg
    16621 atgatgtgta taaaggatta cgttcctctt tttctaataa atccaaacct gacgctgccg
    16681 ccatttcaat aattgcaatt tgttgttcgt ctgcgcgtgc ataacatgct tcaataggtc
    16741 gcattaaagg gtcattaacg atgactgtat acttagtggc acctgttgca tgcattaaag
    16801 catctgtggt accttcacca ccatcagcca tcggaatgat atcataatga agggtattcc
    16861 cataaacatt agtaaaagcc tgttttataa tattgccaac ttgctgtgcg gtcatacttt
    16921 ccttaaatga gtcaggagcg atgacaattt tttgtaattg catagtagac atcctctcat
    16981 tactgttgtc gctcttattg tatataaaat tactagtgaa ctaaagtaat ctctatagtg
    17041 ctaatgagtc acgcgataaa tcagttacat tataatggcg tataaggatg aggggaaaga
    17101 gactaaattt gctgtgaaag ggtataaaga ttgaaataag catatttgaa gtgaagcgga
    17161 ttggctcgca tattcgagta tgttcgagaa cgtagttatg catatatttt gatgttgagg
    17221 agttacacat atgaaactaa cacaaacgca tgctgaaatt ttaaaattta tcattgttgg
    17281 cggcattaat acgttaaatt attatgttgt ctatttattg ctgttaaaat tattacacat
    17341 tgaatatatg attagtcata ttaccggatt tctagtcgct tttgtgattt catattattt
    17401 gaattgttat tttgtttaca gggtaaaacc tacttggaga aaattcatta gttttccaat
    17461 tacgcagatt gtcaacgtaa gcttacaaac agttttatta tatgtatttg tatcctggct
    17521 aaatttgcca gcagaaattg caccatttgc tggtttaatt attacgatac ccatcacatt
    17581 tatattatct aaatggattt taaaagatag caattaaagt agcgtaaacc actaaaaaaa
    17641 actttactta agccttaata ttgcgtgaaa tataataaat aaatgatata atatgatggt
    17701 cgcctaacat ctcgggcgtt actttaacgc agagaggagg tgtatgacgg atgttcaatt
    17761 tattaattaa catcatgact tcagctataa gcggctgtct tgttgcgttt tttgcacatt
    17821 ggttacgaac gcgcaacaat aaaaaaggtg acaaataacc aacacctaaa taaatcccct
    17881 cactatggca gtagtgaggg gatttggtgt atttagatgt tcaatttatt atgtaattaa
    17941 ttatagcatt gatgaacaag gtgtgcaatg tatttttgca atattacaat aaatgaattt
    18001 aattatatta gaattaagta tttcgaaaaa tgatttaata gttgagtcat aggatcatta
    18061 gataaagcga ataatcaatt atagggacat tgggatacaa atcaatgtac tagactctac
    18121 aaggctatat tcagggaccc aacaaagaga aattggattc ccaatttcta gagacaatgc
    18181 aagttggggt gggacgacga aataaatttt gtgaaaatat catttctgtc ccactcccag
    18241 aaccactaca taataaatca ttagtggctc tttatcattt ctgccccata accttgtgaa
    18301 tttttaaaag aaatgtgtca caaatccacg cgacattgat aatcgttatc aactaatata
    18361 tgatgtactt gtaataagta ttaattttta acaaataaca aatttatatt atgtatatca
    18421 tccagtgaaa taaataatat aagcggctta atttagatta ttaattgaaa ggagttacat
    18481 atacgatgaa caatcagaca aattggataa agatactcag cggatttgct tctgatagta
    18541 aatggaaaat tatgttatca attttgttgt ctatcatcag tgtcttttct ggattagttc
    18601 cttattgggc agtatttaaa attattttaa tgatgattaa caatacatat acgattaatt
    18661 cgattatggt ttatatcttt attgctttaa tcgcttatat ttcacaagtg tgttgttttg
    18721 gagcgtcaac gatgctatca catattacgg catatgaaat tttatctgaa attcgtaaaa
    18781 aattagctca aaaattaatg cgtctccctt taggcgtagt ggaatctaag aaaataggtg
    18841 aattaaaaaa tatatttgtc gataaggttg aaacaataga attaccttta gcacatatga
    18901 ttcctgaagt tataggaaac ttacttttgt cagctgctat ctttttatac ataatgctca
    18961 ttgattggcg tatggctagt gccttattag taacgatacc aatttcaatt ttcgctttta
    19021 aaaaagttat gtctggattt aatgagacat atgctgaaca aatgaaatcg aataattata
    19081 tgaatagcgc gattgtagag tttatcgaag gcattgaagt gattaaaaca tttaatcaat
    19141 ctcaaagttc atataaaaaa tataaagatg cagtagataa ttataagatt cacactttga
    19201 attggtttaa aaatacatgg gggtatatga atttaggagc tagtgtatta ccttcaacat
    19261 ttttagggat tttaccggtc ggcatgtatt tgatatctat caaccaatta aactatgcgg
    19321 aatttttcct atgcatcgta ttatctttag gtgtagtagc accgattaag aattttacta
    19381 attatgtaaa tcatttaaag tctatacaat acgcattaac tgaagtgaat caaatattaa
    19441 gtctagaaga gttagtattg tcaacaaaat ttaagaagcc tcaacattat gaaattgctt
    19501 ttaataatgt tggattttca tataacaaag ataaggacga tctggtcttt aagcatttat
    19561 catttacagt accagaaaat aatttcacag ctatcgtcgg ggcatctggt agcggtaagt
    19621 caaccattgc caagcttata tcacgatact gggatgtgac ttcaggtgaa attacgattg
    19681 gcggtataaa tataaaagat attgaatcga aacaacttaa cgatttagtt ggttttgttg
    19741 gacaagataa ctttttatta aatcttactt ttaaagaaaa tattaaactt ggcaatccag
    19801 aagctacgga tgaggcagtt gaaaaagctg ccaagttagc acaatgtcat gaatttattg
    19861 aaaagttgcc agatggatat gatacgaatg ttggtacagt gggagataaa ttgtctggtg
    19921 gtgaaaaaca acgagtcact attgcaagaa tgatattaaa agatgcgcct atcattgtat
    19981 tagatgaagc gactgtttac gttgatccag acaacgaaca aaaaattcaa gaagcattaa
    20041 atgtgttgac gcaggataaa acattgattg ttattgcaca tcggttatct acgattcaac
    20101 atgcagatca aattattgtt ttaggtaaac aacaaatttt agaaaaaggg tcacatcact
    20161 tattgctaaa attaaacggt aattataaga aaatgtggga tacgcacatg catacaaaag
    20221 attggggaat caatactgga cataattaaa ctaatgagaa aaggaggtca aatatgtttc
    20281 aaattacatt taaaatttta aactggataa gaccatataa agcaagaatg attttaggat
    20341 ttagcatgtc atttttaaat gctattttta ttgcgttacc tatcttttta gctgcaaaaa
    20401 tatttaataa tgtgctatct cataaaccta tttatatgaa agatatattg aatgttgtaa
    20461 ttatcatggt tttattagtg attggacgat ttattacagc atatttcaaa agcaaaagcc
    20521 atgagagcat cgcttatgaa atgagtgcga aagaacgttt agatataggg gataaattga
    20581 aaaatgtaag gttaggttat tttaattcgc atcattcaaa tgagttaaca acaatagtaa
    20641 caacagattt aaccttttta gaaaactttg ctatgaaaat ggtggacgtt gttgttaatg
    20701 gatacatatt aattacagta ctcatattgt ctctacttgt agtttcgtgg caagtatcat
    20761 tattagcatg cattggcgta ttactatcat tttttgcgat tcaattatta gaaagaaaga
    20821 gtcgacaaaa tgcgccagcg tatcataatg tacaaaacca attagtggaa aaggtattgg
    20881 aggttattcg tggtattcaa gtaataaaat cattcgcgaa agaaaatacg agtcttaaaa
    20941 gttttaacca agcagtcaat gaaagtaaac gtataaatac aaaaatagaa atgcaatata
    21001 tcccattcaa tttattgcat ttacttagtt taaaagttgt ttcaataatg attgtattag
    21061 ttgcatgctt attatatatg aatcatagta ttgatttacc tacccttatt atgatttcaa
    21121 ttttttcatt tgtgatattt gatagtgttg aaaatattaa tagtgctgca cacgtacttg
    21181 aaatgataga tatgacgata gatgatattg aaaagataaa aaatgctcca gaactggatg
    21241 agaatggaaa aaatttgacg attaaaaatg aaaatatcgc ttttcaaaac gtgaactttt
    21301 catatgatga taaacaagtg ataaagaatg tgaattttga gatacctaca caaacatcaa
    21361 cagcaataat tggaccttca ggaagtggga aatctacatt atgtcactta ctcttgcgct
    21421 tttatgatat cgatgatgga aatattcgca tcgatggtgt tgatattaaa gatatgacat
    21481 taagtacgtt aatgtcgaaa attagtgcag tatttcaaaa ggtgtattta tttaatgata
    21541 cgattgaaaa taacatattg tttggcaatc caggtgcaac gaaagaagaa attattcgtg
    21601 cagcgaagca agcatgttgc cacgacttca tcatgtcatt acctgaggga tatcaaacaa
    21661 tgctaaatga aaaaggtagt aatttatctg gcggagaaaa gcaaaggatt tctattgcta
    21721 gggcgatatt aaaagatgca ccaataatta ttttagatga agcaactgca agtattgacc
    21781 ctgaaaatga acagctgatt caaacggcaa ttaatgaatt aagtaaaggc aaaacagtaa
    21841 ttacaattgc acataaactt gaaactatta aaaatgcaga tcagattata gtgctcaatg
    21901 aaggtgaaat aattcaaaaa ggtagtcatg atgaattaat tcgaaaacca ggaatgtatc
    21961 aagactttat aagaataaaa agtaaatcag caggctggaa attataaata atagtttaat
    22021 atggaatcag aatttggaaa taaaaagggt gatagataat caacagcaaa ataaatcccc
    22081 tcactagggc aatagtgagg ggatttggtg tatttagatg ttcaatttat tatgtaacta
    22141 attatagcat tgatgaacaa ggcgctcaac gtaatattac aattaatgga ttgtttcaca
    22201 agatggtaaa gctaggatgt ctttttatta agaaataatg caattattaa ttttaactga
    22261 catattttat cgaatcgctg tttatatctg cccgaattcc ttaagaagtc ttagtcattt
    22321 caatagcaag gtcaaaattt tgccaaaatt tatagttata ctgagaagtt taaattcatg
    22381 ttttcttgaa aaacatgcgc cttaaatgta aacttattaa ttataaaagt ttacatttgg
    22441 attgaggtgg ttatttttat gaggattttt attacaagta cgaatactga tgtaggcaaa
    22501 acctatgtta caaagcattt ataccatgct ttgaaaacac gtggtcatcg agtttgtatt
    22561 tttaaaccat ttcaaactga ggaacgccaa gacgggacgt ttccagattt agaagtattt
    22621 aaaaatgaat gtgatttaag ctatgacata acgtcacttt atacttttaa gcaacctgta
    22681 tcaccacacc ttgcatttaa aatgacagat caaatttttc taaataagca gcgtgtatta
    22741 gataaggtaa aagttttaga taaggaattt gattttatct taattgaggg tgctggggga
    22801 attgccgtac caatatatga aggtacagat gatttctaca tgactaaaga tctaatcaat
    22861 gattgtgcag attgtgtcat cagtgtgttg ccatcaaaat taggtgctat tagcgatgcc
    22921 attgttcacc aagattatgt taatcagaat gtatcggcga gtaatttttt aataatgaat
    22981 cgctatacag acagctatat tgaaaaagac aatcaaatga cgattggaaa attaacaaat
    23041 aaaacagtct atacatttga agaacatgcc acgtatgaaa atttctcaga agcattttta
    23101 aaacaattaa taggagttaa aaatgaatta cacacaacaa cttaaacaaa aagactcaga
    23161 atatgtttgg catccattta cacaaatggg tgtatatagc aaagaagaag caatcatcat
    23221 tgaaaaagga aagggtagtt acctttacga tacgaatggc aataaatatt tagatggtta
    23281 tgcatcgttg tgggtcaatg tgcatggtca taataacaaa tacttaaata aggtaattaa
    23341 aaagcaactc aataaaattg cccattctac gctgctagga tcatcaaata ttccgtcaat
    23401 agaacttgcg gaaaaattaa tcgaaatcac gccaagtaat ctaagaaaag tattttattc
    23461 tgatacaggc agtgcgtctg ttgaaatcgc aataaagatg gcatatcagt attggaaaaa
    23521 tattgataga gaaaaatatg ccaagaaaaa caagtttata acgctaaatc acggttatca
    23581 tggggatacg attggtgcgg taagtgttgg tggtatcaag acctttcata aaatatttaa
    23641 agacttaata tttgagaata ttcaagtaga aagcccatct ttctatcgca gtaattacga
    23701 tactgaaaat gaaatgatga cagctatttt aacgaatata gagcaaattc taattgaaag
    23761 aaatgatgaa atcgcagggt ttatattgga accgttgatt caaggtgcga caggcttgtt
    23821 tgttcatcct aaaggctttt tgaaagaagt cgagaaattg tgcaaaaaat acgatgtctt
    23881 attaatttgt gatgaggtag cagttggttt tgggagaact ggaaagatgt ttgcatgcaa
    23941 tcatgaagat gttcaaccgg atattatgtg tttaggtaag gcgattactg gtggctactt
    24001 accacttgca gctacattga catctaaaaa aatatacaat gcatttttaa gtgattcgca
    24061 tggtgtgaat acctttttcc atggtcatac atacaccgga aatcaaatcg tttgtacggt
    24121 tgcattagaa aatataagac tttatgaaaa acgtaagtta ttgtcacata ttgaaacgac
    24181 atcatcaaca cttgagaaac agttacatgc gctgaagcgt catcgaaatg ttggtgatgt
    24241 aagaggacga ggcttaatgt ttggagttga attagttaca gataaagata gtaaaacgcc
    24301 gttagaaatt gaaaaagttg aacgtattgt acgtaattgt aaagaaaacg ggctaatgat
    24361 tagaaattta gaaaatgtca ttacgtttgt gccagtgtta agtatgtcaa ataaagaagt
    24421 gaaaacgatg gtacgtattt ttaaaaaggc agtacataac attttagata ggaagtgtta
    24481 atatgaattt ggctaaacgc atattacaag gggaacaatt aacaaaagag actgtattga
    24541 aaatttatga ggatactaat attgatacct tagatttatt aaatgaggcg tacattttaa
    24601 gaaaacatta ttttggtaaa aaagtaaaat taaacatgat tttaaatgct aaaagtggta
    24661 tatgtcctga aaattgtggg tactgtggac aatcacgaga tattaaacaa aaacagcgat
    24721 atgctttaat tccagaggaa caaattatcg atggagcaaa ggtggcacat gataatcata
    24781 ttggaacata ttgtattgtt atgagtggta gaggaccgag cgataaagaa gttgatcata
    24841 ttagtaatac tgtaagaacg attaaatctc aacacccgca actaaaaatc tgtgcatgtt
    24901 taggattaac gaatgacgaa caagctaaga aacttaagtc agctggtgta gacagatata
    24961 accacaatat taatacaagt gaaaattacc atgataacgt cgtgacaacg catagttata
    25021 aagatagaac agatacgata gaactaatga aagcgaataa tatatcacca tgttctggcg
    25081 tgatttgtgg tatgggagaa tctaatcaag atattgttga tatggcattt gctttaaaag
    25141 aaatggatgc cgacagtatt ccgattaatt ttttgcatcc aatcaaaggc acaaagtttg
    25201 gaagcatgga tgatttaaca ccaatgaaat gtttaagaat cgtagcatta ttccgattaa
    25261 tcaatcctac gaaagaaatt cgtattgctg gaggaagaga ggtcaactta cgttcgttac
    25321 agccattagc attaaaagcg gcgaattcaa tatttgtcgg tgattatcta attactggtg
    25381 ggcaaccgaa ccaattagac tacgatatga ttaatgattt aggctttgaa attgattatg
    25441 acacttgtga aaataaggaa aacaagaatg aagtttcaag agcaaattga tttattacaa
    25501 cagaaagggt tatatcggtc gcttaaatcg gtagggtata tagatagaca gtatattgaa
    25561 gtagaaaata agagatgtac gaactataca tctaatgatt atttaggatt aggtcaaata
    25621 gcgtttgata aggatgattt cgaaagattt atgcggaagt atagttatca cttatcaagt
    25681 tcaagattaa ttagtggaag ttcgacagct tatgaagaaa ttgaaacaat gttagcaggt
    25741 tggctcggat atagtgcatg tactatctta aatagtggtt atgatgctaa tttggcgtta
    25801 tttaatattt tcaaaaatac aaattgtgtc gtgttttcag atcaagaaaa tcatgcgagt
    25861 attattgacg gtattaagtt aagtggttta gaaaaagtga tatataagca tttagatatt
    25921 gctgatttag aaaaaaggtt agagaaatac cctaatcaaa atataccaaa aataatcata
    25981 tctgatagtg tattttcaac gaatggcgac gttgtggata ttggtcaatt agtctcatta
    26041 aagcataaat ataacgcaac actgatactc gatgtttcac atagttttgg aatagagaat
    26101 tactcgaatt atcaaggtgt agatatactc acttctagtt tatctaaagc atgtggtgca
    26161 tacgggggtg tgattttaag ttcaaatgat gtgaaggata tgttaattaa tcacggtaga
    26221 ccactcatct actcaagtag tttgccaatt tataatttgt attttataaa aagaaacatt
    26281 gaaaagttaa taaatgctga tgatagacgc actaaattaa atagtttgag taaatatttt
    26341 aaccaaaagt taaaagcact caatgttaat tataatagtt caaactcgcc gattaagttt
    26401 attgagtttg atgacataga agcggctgag aacattcatc aaacattatt aaagcatcat
    26461 gtgtttacaa gttatttaag gtatccaact gtgactaagc caatgttaag aatatcattg
    26521 tcgtattttc atactgaaca agatatagat aggttgtttg aaattttgca tcaagaagat
    26581 tgaggtgatc gtcatgtata gtataaaaat gcgttctagc aatcaagatg ttcatattag
    26641 tggtgctgaa acgatttgtg agtttgacaa aatagaacag acggtacaaa ggttttataa
    26701 taaaggtttt tttcatgaaa atgggcaacc ggattttctc aatataaaaa tacaaaaaat
    26761 catggaaccg attcaacaaa taaaagcatt acaaattatt gaggatgata aagcaaatct
    26821 ccaacattta acacaagaat gtggtgtcac tgaacaagcg ctaaatcaag ggatgacata
    26881 tattaaaaat gaaacggttt atacaggagc tattattcta tctgctatat ctggtaaacg
    26941 tttagattca tttggacaaa gggggattag ggcaacacat ttttcgtttg aagatataaa
    27001 taataaaggt gatcttaatg aaagagtgac tgatgcctta gcaattgcta gttgtatcaa
    27061 tgcgcatccg tatgtcaaag gagaactttg cgtgtccgat gacttaacgt atacgacagg
    27121 ttattttgcc gctgctaaaa ttggttacca tcgattattt gatattaaac cagttaatac
    27181 gagatatgga ggcagaataa tatttgtgga cgattgtatt gatttaaatc attacatatc
    27241 atttttagaa agcacaccga agcaagttgt ttatgaaacg gtataggggt tttagtatga
    27301 catcaaaaga tattactcaa attagtgtca ttgctgcgat tttaaccatt ttggcagttt
    27361 tgaaaatacc gtccattata ccaggattag attttcaatt atctgcaccg gcagcattat
    27421 tgatattagc tttctttgga attaaaaagt actttttagg tggattatta tctagcctat
    27481 tattactagt atttggcgta tttaatccaa ttaatgtgat tatctctatt atatttagag
    27541 ttatagctat tgcagttgtt tatttattga aaataaatgt actatcatta gttttagcaa
    27601 gtgtattagg cagtttggta tctaggctac tattatctat tattttaaat ttacctgtgt
    27661 gggtagtgtt gttaaacgcg attccaggcg taatattcac tttaattgta gctattcctt
    27721 tatatctcac attgagaaaa agaatggcag tattactaag ataataaatc aaaacacggt
    27781 cgtcacaatt actgttggcg accgtgtttt actagctatt tattgttttc agtttctttt
    27841 gtatctaaca atttcacttt gtgattttcc caatcaattt catatgttga tttaaatgtt
    27901 ctagttttaa agtttttata atttgcgcct gcccagtaga agccattcca acgaatttgg
    27961 tataaatcca tttcacgttg ataagttact gtaattttag attttttagc gccatcttgt
    28021 ctgtgtgata gtacgcttaa aaattctgga ttgaagttac ttctagataa taatggcatt
    28081 tggtgttgcg ctatgaagtt ttggccagcg tatgcactgc tttgtctgcc agctaagaag
    28141 agttcattac catatgttgg gtggaagcta tctcttccat aaggtcccca accattattc
    28201 ataattttat gtgcttcaac tccccagcca acatttttat aatttgtgtt gcgacttaat
    28261 gttgttctgt aactttcttg tttataatta attgtttcag aaaaagctgt atttccatta
    28321 agtccaccag ataaaccatt agagatacta atgtcaccac caaatgtata gcctaaagta
    28381 ttttgaactt gaaactcttc attttgattt tttggtgcat aatcaacgac gtttactgaa
    28441 tcattagatt gtgagcttat agatacattg tatttagctc cccaatataa ttttgaaaag
    28501 tcatagtcat taggattagg tttcacaaag cctgagttaa tattcccagt agctttaagt
    28561 actaaagtat ctttatcata acttttatct ttgatgaaat taaatgttaa aatctgtgaa
    28621 attttaaatt tatcagaatc tgctgtggct gttgttttgt ataaagtaac tttgtcatcg
    28681 acttttttta cgctgactgg tgttatttta ccttcagcat tagcagtacc agaaagtaat
    28741 aataatgcca tagatgtagc aacggatgat ttgactaatt tattcatttt catatcaatt
    28801 ctgtcctttc accttgattt catgagtctt ccaattgacc tcgtatttca cagtatagtt
    28861 tctatttaca aatgcattat ggactctatg tccgtctaaa taactgttgc cataatgcgt
    28921 tgatctttta atggcatgag tgacatccat gtttcttccg taagtaattt caaattcgct
    28981 tgtatcgctt gaaccttttt catgagatac tgtggcgata aatgaagggt taaatccact
    29041 ttgtacaaga ggtggtaact cactgtctgg aacgaaataa tctctaggat ctttactatg
    29101 aggtttgtag cctacaaata aatcgctatc aaaggctgat ttttgacctg attcagtggc
    29161 gaatgaattc gctttgacgc cccataaaac actttttgag ttttgttgtt ctacttcact
    29221 tacataattt tgttgtgtat agctaatcga tttagaatag ttaaatgatc cattaccacc
    29281 gagtgatggg gctgattgga aattaccacc gatattgtat cctaatgtct gactcacgtt
    29341 tgtagattca attttatttt taggtaaata attaattaaa gaaacatatt tatcatttgt
    29401 ttttaaacca atattatatt ggaatggcca tcgcatagct ttaacatgat tagttttttt
    29461 atagttgtaa tatgttgttc tagagctaat gaatccttgc atctttaata tcaaagcatc
    29521 tttgttatat tttttatcct ttacaaaatc aaattgaata ttttgagtca cgccccattt
    29581 attacttgtt ttatcttctg tccttttgat aatttctata tcgcttcctt taccgatgtc
    29641 ttcagtatcg ttagcagctt tagcattttc taataacgga ttggcaagag gggcaagtaa
    29701 gctcacagat aaagttgtag ttaatatttt atttttaagc ataagtttca ctttatttct
    29761 ataattttat ttaacttaat taaataataa tatattttgg gcgttattat aattaactgt
    29821 tcattaacaa ttgaccatta tcaacttttt ttacaattaa gttaaaaatg ccataattcc
    29881 gttcgattta accttttttg tttggaatgc taaaagctaa ttttgtgaga aatatagtta
    29941 ataaaacctt ggtattgttg acagttgtaa acaatttaag gtaaccgatt gcacgaatgt
    30001 cttgctttct tttttaatta atgtttgtaa aatacaaatt gtgcttttta cgatttttga
    30061 tgaatccatc taaaaattaa ttgtttatta acttgataat atttatttaa gctttcaagt
    30121 taaatt
//
LOCUS       DALAAR010000032        29661 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000032 DALAAR010000000
VERSION     DALAAR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.032"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            205..1395
                     /locus_tag="O2458_001415"
     CDS             205..1395
                     /locus_tag="O2458_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ3887282.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1504..2748
                     /locus_tag="O2458_001416"
     CDS             1504..2748
                     /locus_tag="O2458_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCZ3887283.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3251..4180)
                     /locus_tag="O2458_001417"
     CDS             complement(3251..4180)
                     /locus_tag="O2458_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ3887284.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4422..5801)
                     /gene="argH"
                     /locus_tag="O2458_001418"
     CDS             complement(4422..5801)
                     /gene="argH"
                     /locus_tag="O2458_001418"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCZ3887285.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5791..6996)
                     /locus_tag="O2458_001419"
     CDS             complement(5791..6996)
                     /locus_tag="O2458_001419"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCZ3887286.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7347..8678
                     /locus_tag="O2458_001420"
     CDS             7347..8678
                     /locus_tag="O2458_001420"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCZ3887287.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            9003..9578
                     /locus_tag="O2458_001421"
     CDS             9003..9578
                     /locus_tag="O2458_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCZ3887288.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9583..10107
                     /gene="lepB"
                     /locus_tag="O2458_001422"
     CDS             9583..10107
                     /gene="lepB"
                     /locus_tag="O2458_001422"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ3887289.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            10123..10698
                     /gene="lepB"
                     /locus_tag="O2458_001423"
     CDS             10123..10698
                     /gene="lepB"
                     /locus_tag="O2458_001423"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ3887290.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10858..14334
                     /gene="addB"
                     /locus_tag="O2458_001424"
     CDS             10858..14334
                     /gene="addB"
                     /locus_tag="O2458_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCZ3887291.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14335..17988
                     /gene="addA"
                     /locus_tag="O2458_001425"
     CDS             14335..17988
                     /gene="addA"
                     /locus_tag="O2458_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCZ3887292.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18154..19056
                     /locus_tag="O2458_001426"
     CDS             18154..19056
                     /locus_tag="O2458_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCZ3887293.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            19385..19774
                     /locus_tag="O2458_001427"
     CDS             19385..19774
                     /locus_tag="O2458_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCZ3887294.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(19945..21261)
                     /locus_tag="O2458_001428"
     CDS             complement(19945..21261)
                     /locus_tag="O2458_001428"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCZ3887295.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(21313..22137)
                     /locus_tag="O2458_001429"
     CDS             complement(21313..22137)
                     /locus_tag="O2458_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ3887296.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            22251..22559
                     /locus_tag="O2458_001430"
     CDS             22251..22559
                     /locus_tag="O2458_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCZ3887297.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            23106..24920
                     /locus_tag="O2458_001431"
     CDS             23106..24920
                     /locus_tag="O2458_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ3887298.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            25123..27732
                     /gene="clpB"
                     /locus_tag="O2458_001432"
     CDS             25123..27732
                     /gene="clpB"
                     /locus_tag="O2458_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCZ3887299.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(27791..28660)
                     /locus_tag="O2458_001433"
     CDS             complement(27791..28660)
                     /locus_tag="O2458_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3887300.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            28770..>29661
                     /locus_tag="O2458_001434"
     CDS             28770..>29661
                     /locus_tag="O2458_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ3887301.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNI"
BASE COUNT        10373 a         4264 c         5336 g         9688 t
ORIGIN      
        1 gtaaaattat caattctttt gcttgtaatc atttaagtaa ttatgtcatt ataaatgtaa
       61 gggttttcaa aatagacaaa tttaatgaaa cttagtgatt atttcatctt ttttggtgga
      121 aaagtatctg ctttgaaaac taatgtaatt atttggataa tgtttcatgt ataatagtta
      181 taaatgatga aaaggggaga tatgatgact aaatctgaaa aaattattga gttaacaaat
      241 cattacggag cacataatta tttaccattg ccaattgtca tttcagaagc tgaaggggta
      301 tgggttaaag atcctgaagg caataaatat atggatatgt tatctgcata ttccgctgtt
      361 aaccaaggtc atagacatcc gaaaattatt caagcattaa aagatcaagc tgataaagtg
      421 actttagttt cacgtgcttt tcatagtgat aacttaggtg aatggtacga aaaaatttgt
      481 aaactggcag gtaaagataa agctttacca atgaatacag gtgctgaagc agtagaaaca
      541 gctttgaaag cagcacgacg ctgggcatac gatgttaaag gaattgagcc aaataaagca
      601 gaaatcattg catttaatgg taacttccat ggtcgaacaa tggcgccagt ttcattatct
      661 tcagaagcag aataccaacg tggttatggt ccgttattag atggatttag aaaagttgat
      721 tttggagatg tagatgcatt gaaagctgca attaatgaaa atactgcagc agttttagta
      781 gaaccaattc aaggtgaagc gggtataaat ataccgccag aaggatattt gaaagcaatt
      841 agagaattat gtgatgaaca taatgtctta tttattgctg acgaaatcca agcaggatta
      901 ggtcgttcgg gtaaattatt tgctacggat tgggataatg taaaacctga tgtctatatt
      961 ttaggtaaag cactaggtgg tggagtcttc ccaatttctg ttgtattagc agataaagaa
     1021 gtattagatg tctttacacc tggctcacat ggttcaacat ttggtggtaa tccacttgct
     1081 tgtgctgcat caattgctgc attagatgtt atcgttgatg aggatttacc aggccgctct
     1141 ttagaattag gagattattt taaagaacaa ttaaagcaaa ttgatcatcc atcaattaaa
     1201 gaagtccgtg gacgtggttt gtttataggt gtggaactta atgaaagtgc tagaccatat
     1261 tgtgaagctt tgaaagaaga aggcttatta tgtaaagaaa cgcatgatac tgtcattcgt
     1321 tttgcaccac cattaattat tactaaagaa gaattggacc ttgcacttga aaaaataaga
     1381 catgtatttc aataatgtca aaaagttgta acaatcagtt tgtaagggct ttaaaaagga
     1441 atacgttatg ttacaatatg tttgaaagcg aaatcattag gtaaagaaga ggcgaaaagg
     1501 atcatgactg agaacaataa tttagtaact tctactcaag gaattattaa agaagcattg
     1561 cataaattgg gatttgacga aggaatgtac gatttaatta aagaaccttt aagaatgtta
     1621 caagtgcgta tccctgtacg aatggatgat ggcacagtaa aaacattcac aggttaccgt
     1681 gcgcaacata atgatgctgt tggaccaaca aaagggggcg tgcgtttcca cccagatgtt
     1741 gatgaagaag aagtaaaagc attatcaatg tggatgactt tgaaatgtgg cattgtaaac
     1801 ttaccatacg gtggtggtaa gggtggtatc gtttgtgatc cacgtcaaat gagcattcat
     1861 gaagttgaac gtttatcacg cggatatgta agagcaattt cacaattcgt aggtccgaac
     1921 aaagatattc cagcaccaga tgtatttaca aactcacaaa ttatggcttg gatgatggat
     1981 gaatatagtg cattagataa atttaattca ccaggtttca tcacaggtaa accaattgta
     2041 ttgggtggtt ctcatggacg cgacagatca actgcactag gtgtagttat tgcaattgaa
     2101 caagctgcaa aacgtcgtaa tatgcaaatt gaaggtgcca aggttgttat tcaaggtttc
     2161 ggtaatgccg gaagtttctt agctaaattc ttatatgatt taggtgcaaa aattgtaggt
     2221 atctctgatg cttacggtgc attacacgat ccaaatggct tagatataga ttatttatta
     2281 gaccgtcgtg atagttttgg tacggtaaca aatttatttg aagaaacaat ctcaaataaa
     2341 gaattgtttg aattagattg tgacatttta gtaccagcgg ctatttcaaa ccaaattaca
     2401 gaagacaatg cacatgatat taaagctagt atcgttgttg aagctgctaa tggacctaca
     2461 acaccagaag caacacgtat tttaactgaa cgtggtatat tattagttcc agacgtatta
     2521 gcaagtgctg gtggtgtaac ggtttcttac ttcgaatggg tacaaaataa tcaaggttat
     2581 tattggtctg aagaagaagt tgatgaaaag ctacgtgaaa aattagaagc ggcatttgat
     2641 acgatttacg aattgtctca aaaccgaaaa atagatatga gacttgcagc atatatcata
     2701 ggtattaaac gtacagcaga agcagctaga tatcgtggtt gggcataatt aattatcata
     2761 tgtgatttaa cgagcttggg acagaaaaca aagccctaag ctcgttaatt ttattttagc
     2821 agtagttgac tgtaaaacaa tgcccgtgta acacgctctt ttcaaaaata gtcggggccc
     2881 caacacagag gctggtggaa agtcagctta caatagtgtg caagttggcg gggccccaac
     2941 acagaggctg gcggaaagtc agcttacaat agtgtgcaag ttggggtgga gccccgacac
     3001 agagaaatta gctcctcaat ttctacagac aatgcaagtt ggcggggccc caacacagaa
     3061 gctggcgaaa agtcagctta caatagtgtg caagttgggg tgggacgacg aaataaattt
     3121 tgcgaaaata tcatttctgt cccactccca aaaagacagc agtaggataa tttcaatttg
     3181 gaaatatctt actgctgttt tttatagtaa tagctatatt tttgtattta tgttttctag
     3241 tttaacatta ctacttaatg acttctttat atttatcagc gaaatttgta aagacaccat
     3301 caacgccata tttatttaat cgtaacatat cagctttttc attcactgta taaggatgta
     3361 ctataaatcc taagtctttt aaatgatggg tattttgttc agttaaatct gtataatcag
     3421 gacctaatcc aatcgcataa gagcgtatct cttttaagcg ttggtcgtta aattgttgta
     3481 gttcaccttt atcaactaat tttactaatg gcacatgctt attttgacga tgaattttct
     3541 ttaaactttc gtcagaaaat gattgaatca ttacatgtcc attttttaat ttattgttat
     3601 ttaaaaggtg atgctttttc aatgaagcta ataattgttc ttccattcct gggtatacat
     3661 caggtgactt tgtttcaata taatagtttg cattcgggcc ataacgttct aaaatttcat
     3721 ctaaagtggg tactttagca tttttataac ttgctcttgc gtattttgga tattttttat
     3781 taaaccaact tcctgcatct aactgtttta attcatcaag ggtataatcc tcaactttac
     3841 cgtgtccatt tgttgtacgg ttaacagttt catcatgcat agcaactaaa tggccatctt
     3901 tggtacgttg taaatcaatt tcgatataag atgcttttaa ctcattatga ctcttatcat
     3961 atgcttgaaa cgtatgctcg ggtgcatagc cacttgcgcc acgatgtgcg atagtagtga
     4021 atcgctcatt cgttaaattt gtatgccatt gaatagcctg aggtttattt gcaatttgat
     4081 ttgtttgttc agcgccagca gtaggtactg ataaaaatcc catagtaaaa acagcagaag
     4141 cagccataaa tttagtgaag cttttcgaag agttagtcat agtctttatc ctttcatctc
     4201 tttatttgta gtctaacaat tttaaaatac tacaaaaacc tgcttgtgaa aacgctttac
     4261 acatttcttt tacaatttat aacatttttg taatataaat ttaaaagaat tatcaaacat
     4321 tttaggaatc ttaaaaatgt gatgttcaac aagacgaaga gagcattaaa atacccacaa
     4381 tgtaatttta atagacattg tgggtaggta gattaacgta tttattgtga tagtaattgt
     4441 ttagcaacat caagttgttg tttgaccgat gattgacctg ttgaaccgta actttgacgt
     4501 cgttttaaac aattttcagg ctgcaaataa tcgtaaatat cggcatcaat actagaatga
     4561 tgttgttgat atgttgctaa aggaacatct aataaataat gaccttgttg tatacattct
     4621 aagacgattt ttcctacaat ttcatgtgca gttctaaatg gaatattttt agttactaaa
     4681 taatctgcta gttccgttgc atttgaaaaa tcttctttaa cagtttgatt gagtcgttct
     4741 ttattaattg tcatcgtttg aatcatacct tcgaaaatac gtaaagaacc tttaattgta
     4801 tggacagcat cgaataaacc ttctttatct tcctgcatat ctttgttata tgctagaggt
     4861 aatcctttta aagtcataag catgctcatt aaatgaccag tcgttcgacc aactttacct
     4921 ctaattaatt ctgccatatc aggatttttc ttttgtggca taatagatga gccagttgaa
     4981 aatgcatctg ataatgtaat gaatttagct tcgtctgtgg accagaaaat aatttcctct
     5041 gcaaagcgtg ataagtgaac catcgttaaa gaaatattat gcaatgtttc aataatatag
     5101 tctctgtcac taacagcatc taggctattc tcatagagac tgccaaagtt caacaatgct
     5161 gttgtctcgt gtctatcgat agggtatgtg gtaccactta aggctgctgc acctaaagga
     5221 ttaatatcga ttcgttttaa actatcttca aatcgttgtt ggtctcgttg taacatccaa
     5281 aaataagtca taatatgatg tgcaaatgaa attggctgtg cacgctgtaa atgagtataa
     5341 ctaggcataa ttgtatcaac attattggaa gcgatgtcta caattacact ttgtaacgac
     5401 ttaattaatg cgatgatatc ttgcacttgt ttcttagtgt acaagtgcat gtctgttgca
     5461 acttgatcgt ttctactgcg tccagtatgc aacttaccac cagcatcacc gatacgttta
     5521 attaattcat gttcaatatt taaatgaata tcttctaatg atgcactaaa ttgaatttga
     5581 tcttgatgat aatcatgttg aatagatttt agtccttgta taatttgttc gctgtcttgt
     5641 tgactaataa tgccttgatt cgcaagcata gttgcatgtg caatgctgcc ttcgatatct
     5701 tgatctatga gcgtttgatc aaaagtaatg gatgcgttaa agtcgtcaac ccactcttca
     5761 ggttgtactt caaatctacc gccccaagct ttattgctca ttgctatagc ctccatgcaa
     5821 catcgcattt acttgagtag gtaaaccata gatatcgata aagccaacag cagcgtcttg
     5881 attaaatgca tcttcttttg tataagttgc taatttttca tcatataatg tgtaaggtga
     5941 ttttctacca ttcacgatgg cattaccttt gaataattta attctgacat caccacttac
     6001 gtattgctga gtactatcaa taaataattt caagctatca gttaaaggtg agaaccaaag
     6061 tccattgtat agttgttcag caaattgctt ctcaatgatt ggtttaaagt gtgcgacatc
     6121 tttcgttaac gtaatcgttt ctaatgcttt atgcgctttt aaaataactt ctgcagcagg
     6181 tgcctcataa atttctcttg atttgatacc tacaagtcta ttttctacat ggtcaattct
     6241 tccgatacca tgcttaccag ctaatgcatt caacgttaaa attaaatcgt ctaattcata
     6301 tgttttgcca tcaatttgaa ctgggatgcc tttatcaaac gttaaaatga tttcatcagc
     6361 agtatctggt gtttcttcta aagcatttgt tagatcgaac gcatcctctg gtggcgcagc
     6421 ataaggatct tctaaaatac cacattcatt cgctctgccc catagatttt gatcgataga
     6481 ataaggtgaa tcatggttga ttgatacagg gatattatgt ttaattgcat aatcgatttc
     6541 ttcttcacga ctccatgccc actcacgtac aggtgcgaat gctttcaatg atgggtttaa
     6601 tgctttaatg gcaacttcga aacgtacttg gtcattccct ttaccagtac aaccatgtgc
     6661 aatacctact gaatttgttt tctcagcaat ctctactaat tttttagcga ttaatggtct
     6721 tgataaagct gaaactaatg gatatgcatt ttcatacatt aaatttcctt tgattgcata
     6781 acttacatac tcatcactaa attcttttgt tgcatcaata atatgacatt caactgctcc
     6841 catatctaaa gcttttttat aaacgatgtc taaatcttta ccttcaccaa catctaggca
     6901 acaagctaca acgtcgtatc ctttgtcgat aagccattga acggccacac ttgtatctag
     6961 tcctcctgaa tatgctaaaa caattttctc tttcataaaa ttcacctcat tgtagttatc
     7021 tgaatatttc gtatgaacac atgatagcaa acttttaatg aaaataaaac cattatttta
     7081 catatttatt cataaaatgc gatagagata gttagagttg taagtgtaaa tgcttattta
     7141 tcaatgttta aaagtatttt aaaaaacatg caaattgtat aaaaaatgtt tgagtgtttg
     7201 acgccagttt gaaatgtttg ttgaaaaata ggtgtgaaac gtcacataaa tatcaatttt
     7261 attgattaag tacaaacttg tgaattgaat cgcaaacgta aacttagtaa aattaagata
     7321 ttaaatgaaa caggaggctt ttatagatga ctcatattca attagatttt agtaaaacgt
     7381 tagaattttt cggtgaacac gaattaaaac aacaacaaga aattgttaaa tcaattcaca
     7441 aaacaattca tgaaggtact ggtgcaggta gtgacttctt aggctgggtt gatttaccag
     7501 ttgattacga caaagaagaa ttttcaagaa ttgttgaagc atcaaaacgc attaaagaaa
     7561 attctgatgt tttagtagtc atcggtattg gtggttctta cttaggtgca cgtgcagcaa
     7621 tcgaaatgtt aacgtcatca tttagaaaca gcaatgaata ccctgaaatt gtatttgttg
     7681 gtaatcactt atcatcaaca tatacgaaag agttagttga ttatttagca gacaaagatt
     7741 tctctgtaaa cgttatttct aaatctggta caactacaga accagcagtt gcatttagat
     7801 tgttcaaaca attagttgaa gaaagatacg gtaaagaaga agcacaaaaa cgtatatttg
     7861 caacaacgga taaagaaaaa ggtgctttaa aacagttggc tacaaacgaa ggttatgaaa
     7921 cgtttatcgt acctgatgat gtaggtggaa gatattctgt tttaacagca gtaggattat
     7981 taccaattgc aacagctgga attaacatcg aagctatgat gattggtgct gcaaaagcac
     8041 gtgaagaatt atcttcagat aaattagaag aaaacattgc ataccaatat gcgacaattc
     8101 gaaacatttt atatgcaaaa ggttatacaa cagaaatgtt gattaactat gaaccatcta
     8161 tgcaatactt taatgaatgg tggaaacaat tatttggtga atcagaaggt aaagacttca
     8221 aaggtatcta tccttcaagt gccaactaca caactgattt acattcttta ggtcaatatg
     8281 tacaagaagg ccgtcgcttc ttattcgaaa cagttgtaaa agtaaatcat cctaaatatg
     8341 atattactat tgaaaaagat agtgatgatc tagacggatt aaattattta gctggtaaaa
     8401 caatcgacga agttaacaca aaagcattcg aaggtacatt attagcgcat actgatggtg
     8461 gtgttcctaa catggtagtg aacattccac aattagatga agaaactttc ggttacgtcg
     8521 tatacttctt cgaacttgct tgtgcaatga gtggatacca attaggcgta aatccattta
     8581 accaacctgg tgtagaagca tataaacaaa acatgttcgc attattaggt aaacctggtt
     8641 ttgaagactt gaaaaaagaa ttagaagaac gtttataaaa tacattactt caaagattag
     8701 tgaagtttga aaagatagaa ctagacgtta actatttaaa gcatattttc gaggttgtca
     8761 ttacaaatgt aaaaatgtaa tgacaacctc gtttttattt atatgcaaga actaggttac
     8821 tagctaatgt gacaagatgt taagagaaaa ttaaagataa aataatatct gccttacaat
     8881 aatattgtta tactactaga gactgattta ttagcatgat tacatgttaa tgtttcttta
     8941 cttagtaatt aactttataa tgtaagaata attatcttca accaaagaaa gggattgatg
     9001 atttgtcgtt tcatcaagta gaagaatggt ttgagatatt tcgacagttt ggttatttac
     9061 ctggatttat attgttatat attagagcta taattccagt atttccttta gcactctata
     9121 ttttaattaa cattcaagct tatggaccta ttttaggtat attgattagt tggcttggat
     9181 taatttctgg aacatttaca gtctatttga tctgtaaacg attggtgaac actgagagga
     9241 tgcagcgaat taaacaacgt actgctgttc aacgcttgat tagttttatt gatcgccaag
     9301 gattaatccc attgtttatt ttactttgtt ttccttttac gccaaataca ttaataaatt
     9361 ttgtagcgag tctatctcat attagaccta aatattattt cattgttttg gcatcatcaa
     9421 agttagtttc aacaattatt ttaggttatt taggtaagga aattactaca attttaacgc
     9481 atcctttaag agggatatta atgttagttg tgttggttgt attttggatt gttggaaaaa
     9541 agttagaaca gcattttatg ggatcgaaaa aggagtgaca tcgtgaaaaa agttgtaaaa
     9601 tatttgattt cattgatact tgctattatc attgtactgt tcgtacaaac ttttgtaata
     9661 gttggtcatg tcattccgaa taatgatatg tcaccaaccc ttaacaaagg ggatcgtgtt
     9721 attgtaaata aaattaaagt tacatttaat caattgaata atggtgatat cattacatat
     9781 aggcgtggta acgagatata tactagtcga attattgcca aacctggtca atcaatggcg
     9841 tttcgtcagg gacaattata ccgtgatgac cgaccggttg acgcatctta tgccaagaac
     9901 agaaaaatta aagattttag tttgcgcaat tttaaagaat tagatggtga tattattccg
     9961 ccaaacaatt ttgttgtgct aaatgatcaa gataataaca agcacgattc aagacaattt
    10021 ggtttaatcg ataaaaagga tattattggt aatgttagtt tacgatacta tcctttttca
    10081 aaatggactg ttcagttcaa atcttaaaaa gaggtgtcaa aattgaaaaa agaaatattg
    10141 gaatggatta tttcaattgc agtcgctttt gtcattttat ttatagtagg taaatttatt
    10201 gttacgccat atacaattaa aggtgaatca atggatccaa ctttgaaaga tggcgagcga
    10261 gtagctgtaa acattgttgg atataaaaca ggtggtttgg aaaaaggtaa tgtagttgtc
    10321 ttccatgcaa acaaaaatga tgactatgtt aaacgtgtca tcggtgttcc tggtgataaa
    10381 gtagaataca aaaatgatac attatatgtc aatggtaaaa aacaagatga accatattta
    10441 aactacaatt taaaacataa acaaggtgat tacattactg ggactttcca agttaaagat
    10501 ttaccgaatg cgaatcctaa atcaaatgtc attccaaaag gtaaatattt agtgcttgga
    10561 gataatcgtg aagtaagtaa agatagccgt gcgtttggcc tcattgatga agaccaaatt
    10621 gttggtaaag tttcatttag gttctggcca tttagtgaat ttaaacataa tttcaatcct
    10681 gaaaatacta aaaattaata tgaaacaaat acaacatcgt ttgtcggttt taatactgat
    10741 aaacgatgtt ttattttgtt agtaccacaa taaaagctaa gttcgaaatg aacttataat
    10801 aaatcaatca caatcacttt gtgttaaaat atgtgtcaaa ggaagtgagg gtttgtcatg
    10861 acattacatg cttatttagg tagagcggga acaggtaagt ctacgaaaat gttgaccgaa
    10921 ataaaacaaa aaatgaaagc agatccgctt ggagatccaa tcattttaat tgcgccaact
    10981 caaagtacat ttcaattaga acaagccttt gtcaatgatc cggaattaaa tggtagttta
    11041 agaacagaag tgttgcattt tgaacgatta agtcatcgta ttttccaaga agttggtagt
    11101 tatagcgaac aaaagttatc taaagctgca acggaaatga tgatttataa cattgttcaa
    11161 gaacaacaaa agtatttaaa actttatcaa tcacaagcaa aatattatgg gtttagtgaa
    11221 aaattaacag aacaaattca agattttaaa aaatatgcag taacgcctga acatttagaa
    11281 cactttattg ctgataaaaa tatgcaaact cgaactaaaa ataagttaga ggatattgct
    11341 ttaatatacc gtgagttcga acaacgcatt caaaacgagt ttattacggg tgaggattca
    11401 ttacaatatt ttattgattg tatgccgaaa tcagagtggc taaaacgtgc tgatatatat
    11461 attgatggtt ttcacaactt ttcaacgatt gagtatttaa taatcaaagg attaattaaa
    11521 tatgcgaaga gtgtcacaat tatattgacg acagatggta accacgatca atttagttta
    11581 tttagaaaac catcggaagt gttacgacat attgaagaaa tagcaaatga actcaatatt
    11641 tctattgaac gtcaatattt caaccaatta tatcgcttca ataatcaaga tttaaagcat
    11701 cttgaacaag aatttgatgc acttcaaatc aatcgagtgg catgtcaagg tcatatcaat
    11761 attttagaat ctgcgactat gagagaggaa ataaatgaaa ttgcgcgacg tatcatcgtt
    11821 gatattcgtg ataagcaatt acgatatcaa gatattgcta ttttatatcg tgatgaatct
    11881 tatgcttatt tatttgattc catattaccg ctttataata ttccttataa tattgataca
    11941 aagcgttcga tgacacatca tccggtcatg gaaatgattc gttcattgat tgaagttatt
    12001 caatctaatt ggcaagtgaa tccaatgcta cgcttattga agactgatgt gttaacggca
    12061 tcatatctaa aaagtgcata cttagttgat ttacttgaaa attttgtact tgaacgtggt
    12121 atatacggta aacgttggtt agatgatgag ctatttaatg tcgaacattt tagcaaaatg
    12181 gggcgtaaag cgcataaact gaccgaagat gaacgtaaca catttgaaca agtcgttaag
    12241 ttaaagaaag atgtcattga taaaatttta cattttgaaa agcaaatgtc acaagcggaa
    12301 actgtaaaag attttgcaac tgctttttat gaaagtatgg aatatttcga actgccaaat
    12361 caattgatga cagagcgaga tgaacttgat ttaaatggta atcatgaaaa ggcggaggaa
    12421 attgatcaaa tatggaatgg cttaattcaa atccttgatg acttagttct agtatttgga
    12481 gatgaaccaa tgtcgatgga acgtttctta gaagtatttg atattggttt agaacaatta
    12541 gaatttgtta tgattccgca aacattggac caagtaagta ttggtacgat ggatttggct
    12601 aaagtcgata ataagcaaca tgtttactta gtaggtatga atgatggaac gatgccacaa
    12661 ccagtaactg cgtcaagctt gattacagat gaagaaaaga aatactttga acagcaggct
    12721 aatgtcgagt taagtccaac atcagatatt ttacagatgg atgaagcatt tgtttgttat
    12781 gttgctatga ctagagctaa gggagatgtt acattttctt acagtctaat gggatcaagt
    12841 ggtgatgata aggagatcag cccattttta aatcaaattc aatcattgtt caaccaattg
    12901 gaaattacta acattcctca ataccatgaa gttaacccat tgtcactaat gcaacatgct
    12961 aagcaaacca aaattacatt atttgaagca ttgcgtgctt ggttatatga tgaaattgtg
    13021 gctgatagtt ggttagatgc ttatcaagta attagagata gcgatcattt aaatcaaggt
    13081 ttagattatt taatgtcagc attaacgttt gacaatgaaa ctgtaaaatt aggtgaaacg
    13141 ttgtctaaag atttatatgg taaggaaatc aatgccagtg tatcccgttt tgaaggttat
    13201 caacaatgcc catttaaaca ctatgcgtca catggtctga aactaaatga gcgaacgaag
    13261 tatgaacttc aaaactttga tttaggtgat attttccatt ctgttttaaa atatatatct
    13321 gaacgtatta atggcgattt taaacaatta gacctgaaaa aaataagaca attaacgaat
    13381 gaagcattgg aagaaatttt acctaaagtt cagtttaatt tattaaattc ttcagcttac
    13441 tatcgttatt tatcaagacg cattggcgct attgtagaaa caacactaag cgcattaaaa
    13501 tatcaaggca cgtattcaaa gtttatgcca aaacattttg agacaagttt tagaaggaaa
    13561 ccaagaacaa atgacgaatt aattgcacaa acattaacga caactcaagg tattccaatt
    13621 aatattagag ggcaaattga ccgtatcgat acgtatacaa agaatgatac aagttttgtt
    13681 aatatcattg actataaatc ctctgaaggt agtgcgacac ttgatttaac gaaagtatat
    13741 tatggtatgc aaatgcaaat gatgacatac atggatatcg ttttacaaaa taaacaacgc
    13801 cttggattaa cagatattgt gaaaccaggt ggattattat acttccatgt acatgaacct
    13861 agaattaaat ttaaatcatg gtctgatatt gatgaagata aactagaaca agatttaatt
    13921 aaaaagttta agttgagtgg tttagttaat gcagaccaaa ctgttattga tgcattggat
    13981 attcgtttag aacctaaatt cacttcagat attgtaccag ttggtttgaa taaagatggc
    14041 tctttgagta aacgaggcag ccaagtggca gatgaagcaa cgatttataa attcatccaa
    14101 cataacaaag agaattttat agaaacagct tcaaatatta tggatggaca tactgaagtt
    14161 gcaccattaa agtacaaaca aaaattgcca tgtgcttttt gtagttatca atcggtatgt
    14221 catgtagatg gcatgattga tagtaagcga tatcgaactg tagatgaaac aataaatcca
    14281 attgaagcaa ttcaaaatat taacattaat gatgaatttg ggggtgagca atagatgaca
    14341 attccagaga aaccacaagg cgtgatttgg actgacgcgc aatggcaaag tatttacgca
    14401 actggacaag atgtacttgt tgcagccgcg gcaggttcag gtaaaacagc tgtactagtt
    14461 gagcgtatta tccaaaagat tttacgtgat ggcattgatg tcgatcgact tttagtcgta
    14521 acgtttacaa acttaagcgc acgtgaaatg aagcatcgtg tagaccaacg tattcaagag
    14581 gcatcgattg ctgatcctgc aaatgcacac ttgaaaaacc aacgcatcaa aattcatcaa
    14641 gcacaaatat ctacactcca tagtttttgc ttgaaattaa ttcaacagca ttatgatgta
    14701 ttaaatattg acccgaactt tagaacaagc agtgaagctg aaaatatttt attattagaa
    14761 caaacgatag atgaggtcat agaacaacat tacgatatcc ttgatcctgc ttttattgaa
    14821 ttaacagagc aattgtcttc agatagaagt gatgatcagt ttcgaatgat tattaaacaa
    14881 ttgtatttct ttagcgttgc aaatccaaat cctaaaaatt ggttggatca attggtgaca
    14941 ccatacgaag aagaagcaca acaagcgcaa cttattcaac tactaacaga cttatctaaa
    15001 gtatttatca cagctgctta tgatgcttta aataaggcgt atgatttgtt tagtatgatg
    15061 gatagcgtcg ataaacattt agctgttata gaagatgaac gacgtttaat ggggcgtgtt
    15121 ttagaaggtg gctttattga tataccttat ttaactggtc acgaatttgg cgcgcgtttg
    15181 cctaatgtaa cagcgaaaat taaagaagca aatgaaatga tggtcgatgc cttagaagat
    15241 gctaaacttc agtataaaaa atataaatca ttaattgata aagtgaagag tgattacttt
    15301 tcaagagaag ctgatgattt gaaagctgat atgcaacaat tggcgccacg agtaaagtac
    15361 cttgcgcgta ttgtgaaaga tgttatgtca gaattcaatc gaaaaaagcg tagcaaaaat
    15421 attttggatt tttctgatta tgaacatttt gcattacaaa ttttaactaa tgaggatggt
    15481 tcgccttcag aaattgccga atcataccgt caacacttcc aagaaatatt ggtcgatgag
    15541 tatcaagata cgaaccgagt tcaagagaaa atactatctt gcatcaaaac gggtgatgaa
    15601 cataatggta atttatttat ggttggagat gttaagcaat ccatttataa atttagacaa
    15661 gctgatccaa gtttatttat tgaaaagtat caacgcttta ctatagatgg agatggcact
    15721 ggacgtcgaa ttgatttgtc gcaaaacttc cgttctcgaa aagaagtact gtcaacgact
    15781 aactatatat tcaaacatat gatggatgaa caagtcggtg aagtaaaata tgatgaagcg
    15841 gcacagttgt attatggtgc accatatgat gaatcggacc atccagtaaa cttaaaagtc
    15901 cttgttgaag cggatcaaga acatagtgat ttaactggta gtgaacaaga agcgcatttt
    15961 atagtagaac aagttaaaga tatcttagaa catcaaaaag tttatgatat gaaaacagga
    16021 agctatagaa gtgcgacata caaggatatc gttattctag aacgcagctt tggacaagct
    16081 cgcaatttac aacaagcctt taaaaatgaa gatattccat tccatgtgaa tagtcgtgaa
    16141 ggttactttg aacaaacaga agtccgctta gtattatcat ttttaagagc gatagataat
    16201 ccattacaag atatttattt agttgggtta atgcgctccg ttatatatca gttcaaagaa
    16261 gacgaattag ctcaaattag aatattgagt ccaaatgatg actacttcta tcaatcgatt
    16321 gtaaattaca ttaatgacga agcagcagat gcaattttag ttgataaatt aaaaatgttt
    16381 ttatcagata ttcaaagtta ccaacaatat agtaaagatc atccggtgta tcagttaatt
    16441 gataaatttt ataatgatca ttatgttatt caatacttta gtggacttat tggtggacgt
    16501 ggacgacgtg caaatcttta tggtttattt aataaagcta tcgagtttga gaattcaagt
    16561 tttagaggtt tatatcaatt tattcgtttt atcgatgaat tgattgaaag aggcaaagat
    16621 tttggtgagg aaaatgtagt tggtccaaac gataatgtcg ttagaatgat gacaattcat
    16681 agtagtaaag gtctagagtt tccatttgtc atttattctg gattgtcaaa agattttaat
    16741 aaacgtgatt tgaaacaacc agttatttta aatcagcaat ttggtctcgg aatggattat
    16801 tttgatgtgg ataaagaaat ggcatttcca tctttagctt cggttgcata tagagctgtt
    16861 gccgaaaaag aacttgtgtc agaagaaatg cgattagtct atgtagcatt aacaagagcg
    16921 aaagaacaac tttatttaat tggtagagtg aaaaatgata aatcattact agaactagag
    16981 caattgtcta tttctggtga gcacattgct gtcaatgaac gattaacttc accaaatccg
    17041 ttccatctta tttatagtat tttatctaaa catcaatctg cgtcaattcc agatgattta
    17101 aaatttgaaa aagatatagc acaaattgaa gatagtagtc gtccgaatgt aaatatttca
    17161 attgtgtact ttgaagatgt gtctacagaa accattttag ataatgatga atatcgttcg
    17221 gttaatcaat tagaaactat gcaaaatggt aatgaagatg ttaaagcaca aattaaacac
    17281 caacttgatt atcgatatcc atatgtaaat gatactaaaa agccctcaaa acaatctgtt
    17341 tctgaattga aaagacaata tgaaacagaa gaaagtggca caagttacga acgagtaagg
    17401 caatatcgta tcggtttttc aacgtatgaa cgacctaaat ttctaagtga acaaggtaaa
    17461 cgaaaagcga atgaaattgg tacgttaatg catacagtga tgcaacattt accattcaaa
    17521 aaagaacgca tatctgaagt tgagttacat cagtatatcg atggattaat cgataaacat
    17581 attatcgaag cagatgcgaa aaaagatatc cgtatggatg aaataatgac atttatcaat
    17641 agtgagttat attcgattat tgctgaagca gagcaagttt atcgtgaatt accgtttgta
    17701 gttaaccaag cattagttga ccaattgcca caaggagacg aagacgtctc aattattcaa
    17761 ggtatgattg acttaatctt tgttaaagat ggtgtgcatt attttgtaga ctataaaacc
    17821 gatgcattta atcgtcgccg tgggatgaca gatgaagaaa ttggtacaca attaaaaaat
    17881 aaatataaga tacagatgaa atattatcaa aatacgcttc aaacgattct taataaagaa
    17941 gttaaaggtt atttatactt cttcaaattt ggtacattgc aactatagta ttttgatttt
    18001 caaaagaata aaaaataatt tcgattaagt gcaaagtcct tgtagcagaa tgaacacaac
    18061 tcattttcaa aattgtctta cttatttatt tgttatttga taacgaaaaa agttataatg
    18121 tgaattaaga taaagatgag gagttgagaa tgaatgaaat tcttatcatt caagtataat
    18181 gacaaaactt catatggcgt taaagtaaaa cgcgaagatg ctgtatggga tttaacacaa
    18241 gtatttgctg actttgcaga aggagatttc catcctaaaa cattgttagc tggtttacaa
    18301 caaaatcata ctttagattt tcaagaacaa gtacgtaaag cagttgtagc agcagaagat
    18361 agcggcaaag ctgaagacta taaaatttca tttaatgaca ttgaattttt accaccagta
    18421 acacctccga ataatgtgat tgcttttggt agaaattaca aagatcatgc gaacgaatta
    18481 aatcatgaag tggaaaaatt atatgtattt acaaaagcag cgtcatcttt aacaggagat
    18541 aatgcaacaa ttccaaatca taaagatatt actgatcaat tagattatga aggtgaatta
    18601 ggtattgtta ttggtaagtc tggtgaaaag attccaaaag cattagcttt agattatgtt
    18661 tacggctata caattattaa cgatatcact gatcgcaaag cacaaagtga acaagatcaa
    18721 gcatttttat caaaaagttt aactggcggt tgcccaatgg gtccttatat cgttactaaa
    18781 gacgaactac cattacctga aaatgtaaat attgttacaa aagttaacaa tgaaattaga
    18841 caagatggta acactggcga aatgattctt aaaattgatg aattaataga agaaatttca
    18901 aaatatgttg cactacatcc gggagatatt attgcaactg gtacaccagc aggcgttggt
    18961 gcaggtatgc aaccacctaa atttttacaa ccaggtgatg aagttaaagt gactattgat
    19021 aatattggaa cgctgacaac ttatatcgct aaataattat catttaaaaa gctaaccagt
    19081 ctttatatag attggttagt tttttcttgc ttttctaaaa aggtgttaaa gataaattat
    19141 ttataatgtt accattttga gatgaaagtg aaatattgat attaagaagt agttgattat
    19201 tttacagcag attcacaata ttctaataag gaataaaatg tcatgttctt cctctcaaat
    19261 atagaagtgt ggtagaatat atattcgtgt ataatcaaat catgattaaa ttacaagcaa
    19321 gtgggtatta atcccaagaa gctttaattt aataatataa ataattaaag tggggggaat
    19381 cagtatgtta catttacata tattaagttg ggtattagcg attattttat ttatcgctac
    19441 atacttaaac atttcaaaaa atcaaggcag atcaccattt ttcaaaccgt tgcacatgat
    19501 tttacgctta tttatgctgt tgacgttaat ttcaggattt tggatattaa ttcagtcatt
    19561 tatgaatggc ggggcaaatc atatgttgct tacattgaaa atgctgtgtg gtgttgcagt
    19621 agttggattg atggaagtgt cgattgctaa aagaaagaga catgaacaaa gtcacacaat
    19681 gttttggata acaattgcat taattatcat cacaatggta ttaggtgtca ttctaccgtt
    19741 agggcctata tcaaaattat tcggtattgg ctaattgata agacatatag tatatgacat
    19801 aaggcatgga caattattag tccaagacct tatgtctttt ttgattctaa aaatgttaaa
    19861 cataaaaaat ggactggata gtagtaccag tccaacataa ttctaaccta gacaattaag
    19921 attagattga atcgcatgat tcatttattt agctttgtaa ccaatcatat tgattaaatc
    19981 tttagggtgg ctatatggtg gtgcataagc cacttcaaac tcagttaact catctacagt
    20041 tagctggttc atcattgcca tcgatagtac atcaatacgt ttatctgcac cttcttttcc
    20101 tactgcagct gctcttaaaa tctgacggtt tgaagtgtca taatatactc ttaagtgtaa
    20161 aggggaattt cctgggtaat aattcgcgtg tgcaccttga gtgacttcta ccattttata
    20221 gtcaaattgc tttagttcgt ttggtttaac gccgacactc gcaaatgtat aatcaaagaa
    20281 cttcacaata ttgttgccta agaagccttt gaattcaata gtgtcatttc cagcaatttg
    20341 ttcggcaaca atacttgctg cacggtgagc gccccaagct aaaggaacac tagccggtag
    20401 atcgacatgt cgataatgtg atgttgcaat atcgcctatt gcataaatgt ttggaacatt
    20461 tgtttcaaat ttatcgttta ccggtatgaa accttttcga tcaagtttga tatttgaact
    20521 ttcgataaat tttgaattgg ggtgagtacc gacaccttca ataatcatat cgtaatgttc
    20581 aacttttcct gatttaaatg taatttcatt tccattgata gcattaattt cctcatttaa
    20641 acggtatgga atctcccgct tatctaattc atcaagtata ggttgattca tgtcggcatc
    20701 cattaattta tttatcttat cagatcgatg aattaaagta gggtgtaaac cacgttcata
    20761 aagattttca agaacttcta atgaaacata acctgcacct acaaccaata ctttatcaac
    20821 ttgatttgct ttgatgaatt gatcgatagc atcagtgtct tctaaatttc taagtgtaaa
    20881 tgtaatatca ctttcaaagc caaggctatt tgcacttgca ccagggctta aaatgagttt
    20941 atcgtaagat tcttcaaatt gttcgtttgt ctttctattt aatacagata cagtttgtct
    21001 ttcatcattg attgcaataa cttcatgata agtttttact gtaatttgct ttctatcata
    21061 aaatttttca ggtgtatacg ctaaagcata tcttctatct tcaacaactt cgccaatgac
    21121 ataaggcaat gcacaattag caaagctcat atcacgatct ttttcaaaaa taataatgtc
    21181 actttcttta tctaaacgtc gaatttggct ggcacatgtt gcaccgccag cgactgctcc
    21241 gactacgact attttgggca tcgtaactta cctcctattg ttttaaaata ttataaaatg
    21301 attagttata aattaacagt aatatctaat atttaaatta aagaaatcat tcaaatatcg
    21361 accaatgcca tcttcattat tgttgaatgt aatattgttc gctacatctt taagttcttg
    21421 caaaccattt tccatagcaa caccgtgacg cgcgtactca atcatttcaa tatcattatc
    21481 ttcatcaccg aatgcaataa tattatttcg gtcaatattt aaaaattgtc taacttgctc
    21541 aatgcctctt gctttattaa taccaagttt tacaatttca atgacaggga atggtgcgcc
    21601 ccagcgtcga tgctcaatat gatcggcata aaaatgagta agcatatttt tgatttcagg
    21661 tattttactt tcttcggctt caattaaaat tgaggtaggg gattctttca agtggacaag
    21721 taaattacca gtttgaattc ttggattacc cattgaaaaa ccttcaaata atcttggatc
    21781 atgattgtta atgaaaacat aatctttcac ttctgctata atattcgata cttgatattg
    21841 ttgtaatcct tgaataatgt tttgtgcgat gcctaaatct aaaatttcat ggcaagtttt
    21901 gaagttttta tctttagggt gatgtacgta agcgccatta aaattaacaa ttggtgtcgt
    21961 taaatttaat tcatgataat acatttgact tgcacgataa ggtctgccag tcgcaatcat
    22021 aatttggtgt ccacgttgtt gtaattcatt taatacttgt ttagtatatg atgaaatttc
    22081 tttgttatcg tttaataatg ttccgtctaa gtctagacat attaaatgtg gttgcatatc
    22141 tctctcctca taagttgctt agttcatata gatacatcat agcattttat tggcgttttg
    22201 tcggatattt gatatattat gaataatatg acattaaaga ggtgatatgg atggaagagg
    22261 cattgaaaga tagtatctta ggtgcattag aaatggtaat tgaccctgaa ttaggaattg
    22321 atatcgttaa tttgggttta gtatacaaag tgaatgttga tgatgaaggc gtatgtacag
    22381 ttgatatgac tttaacatca atgggatgtc caatgggacc tcaaattatt gatcaagtta
    22441 aaacagtatt agcagagatt cctgaaattc aggatactga agtgaatatc gtatggagtc
    22501 caccttggac aaaagatatg atgtcacgtt acgctaagat tgcacttggt gtgagctaac
    22561 ataaacttgc attgcctgta gaatttcttt tcgaaattct ctgtgtttgg gcccgcagtc
    22621 aatataacat tgtagagtat aattcattgt gaataagctc ccttcaaagt agacattgaa
    22681 aaatgaaaat tttgaaggga gccttattta tgttctagtt tctgatttta ttttgaaaat
    22741 ttatatctaa cttgtttcaa aaaatatgaa ttaagacagt gacttgcact tccaagagtg
    22801 tacaattcat tttctagttg aatattttct tgaaaatttt tataataaat ttaatttata
    22861 ttacagttat attacaatac ataatacttc atagttttta agttgccgag ttgcattaca
    22921 aattggattt cgaagtaaga atcatcattg gtaatttttt taatttaaca gtattttagt
    22981 caatatgatt ttgatgatgt cgtaattcaa taatatacat atgcatttca atattctttt
    23041 aaacagcata actaaattta attaagcatc taagaagaac agttttaaaa gggaagaatg
    23101 caaaaatgaa caaaacaaag ggttttacaa agtataagaa aatgagatat attccagggc
    23161 tcgatggttt gagggcaatc gctgttctag gaattattat ttaccactta aataagcaat
    23221 ggttgacagg tggcttttta ggtgtggata cattttttgt gatctctggt tatttaatta
    23281 caagcttatt actcaaagag tatgatgaca caggtatcat taaattgaaa agcttttgga
    23341 tacgtcgttt aaaacgttta ttaccagcag tcatagtttt attaatggtt gtagggacag
    23401 caaccttatt attaaaatca gataatatca ttagggttaa acatgatatt attgctgcga
    23461 tattttatgt atcaaactgg tggtatatag caaaagatgt taattatttt gagcaatttt
    23521 catttatgcc attaaagcat ttatggtctt tagcaattga agaacagttt tacatatttt
    23581 tcccagttat tttggttaca ttattgttaa caattaaaaa gcgatacaaa ataggattta
    23641 ttttttgggg agtatcaata atttctttag ggttaatgat gtttatctat agtattaatg
    23701 gggatcattc acgagtgtat tttggtacag atactagatt acagacattg ttactgggtg
    23761 ttattttagc ttttttatgg ccaccgttta aattgaaaaa tgatccacct aaagttgtaa
    23821 aatatgttat tgatagcata ggtagtttat catttatagt acttatatta ttatttttca
    23881 ttattaatga tgagacgaat tggatatatg atggtggttt ctatttaata tccatattaa
    23941 cgttatttat tattgctagt gtcgttcatc catctacatg gatagcgaag atattttcaa
    24001 atccagtgtt agtatttatc gggaaaaggt cttatagttt atatttatgg cattttgcag
    24061 taattagttt cgtacatagt tactatgtag acggacagat acctgtatat gtgtacttta
    24121 tagatataag tttaacaatt atatttgcag agctatcata tcgctttata gaaactccat
    24181 ttagaaaaga aggtattaaa gctttaaatt ggcgaccttc ttatatacca caatttataa
    24241 gaatggcaat tgtagtaacc ttgttaattc catttatgtt gattttagta ggtgcattca
    24301 ataaatatgg taaagacatt attggagaaa aagcgaatag ctttgatacc acaattgaag
    24361 ataattattt aatgcggata gcaccaattg ataacataca cattgatggc ttagtaagtg
    24421 agaagaaaaa ggaatcttcc gacgtatata ataatattaa acctctttta atcggtgatt
    24481 cagtaatggt tgatatcggt gagtcattta agtcatcagt tcctaagtct agaattgatg
    24541 gaaaagtagg gcgtcaattg tatcaaacct tacctttagt taaagcgaat tattcacaat
    24601 ataaaaaatc atctgatcaa gtcgtattag aattaggtac aaatggcgac tttactgtca
    24661 aacagctcga cgatttactt aatcaatttg gaaaagccaa gatttattta gttaatacac
    24721 gtgttccaag aatttatgag gcaaatgtaa atcgattatt agctgacgcg gcgaaacgaa
    24781 agtccaatgt cacattaatt gattggtata agcgatcaca aggacatagt gaatattttg
    24841 caccagacgg tgtacattta gagtacaaag gagtcctagc tttaaaagat gaaatattaa
    24901 aagcacttaa aaagaaataa catatgttta ataagtctag ttaatgtgta acgtaacatt
    24961 agctagattt ttttattcaa aaaaatattt acaaatatta ggaaatttaa gtgtaaaaga
    25021 gttgataaat gattatattg ggactataat ataattaagg tcaaagaaag tcaaagtcaa
    25081 aagtgcaact tgaccaactc tacaaattag aggtgaaatt atatggatat aaataaaatg
    25141 acatatgctg ttcaaagtgc tttacaacaa gcagttgaac tgagtcagca acataaatta
    25201 caaaatatag aaattgaggc aattttaagc gctgccttaa atgaaagtga aagcttatat
    25261 aaaagtattt tagaacgagc aaatattgag gtagatcaat taaacaaagc ttatgaagac
    25321 aaactaaaca cgtatgcatc tgtagaaggt gacaatatac aatatggtca atatattagc
    25381 caacaagcaa accaattgat aactaaggct gaatcataca tgaaagaata tgaagatgaa
    25441 tatatttcaa tggagcatat tttacgttcg gcaatggaca ttgatcaaac aacaaaacat
    25501 tatataaata ataaagtaga agttatcaaa gaaattatta aaaaagtaag agggggaaat
    25561 cacgtgacat cacaaaatcc agaagttaat tacgaagcat tagctaaata tggccgcgac
    25621 ttagtagaag aagttagaca aggtaaaatg gatcctgtta taggaagaga tgaagaaatt
    25681 cgaaatacga ttcgtatttt aagtcgtaaa actaaaaaca accctgtgct cattggtgaa
    25741 ccaggtgttg gtaaaactgc aattgttgaa ggattagcgc aacgtatagt taagaaagat
    25801 gtgccagaat cattattaga taaaactgtt tttgagttag atttaagcgc attagtagcg
    25861 ggcgctaaat atcgtggtga atttgaagag agattaaaag cagtcctaaa agaagttaaa
    25921 gagtctgatg gtagaattat attatttatt gatgaaatcc atatgcttgt aggtgctggt
    25981 aaaacagatg gtgccatgga tgcaggcaac atgctaaaac caatgttagc acgaggagag
    26041 ttacattgta ttggtgcaac aactttaaat gaatatcgag aatatattga aaaagattcg
    26101 gcattagagc gtcgtttcca aaaagtagca gttagtgagc ctgatgttga agatacaatt
    26161 tcaattttac gtggtttaaa agaacgatat gaagtgtatc atggtgtgcg tattcaagat
    26221 agagccttag ttgctgccgc tgaattgtct gatcgttaca tcactgatcg ttttttacca
    26281 gataaagcga ttgatttagt tgaccaagca tgtgcaacaa ttcgtacgga aatgggatca
    26341 aatccaactg aattggatca agttaataga cgtgtcatgc aattagaaat tgaagaaagc
    26401 gcacttaaaa atgaatctga caatgcgagc aaacagagat tacaagaact acaagaagag
    26461 cttgccaatg aaaaagagaa acaagcagca cttcaatctc gtgtagaatc agaaaaagaa
    26521 aaaatagcaa atttacaaga aaaacgtgcg caactagatg aaagtagaca agcgttggaa
    26581 gatgcacaaa caaataacaa tttagaaaaa gctgctgaac tacaatatgg aacaattcct
    26641 caattggaaa aagaacttag agaattagag gataatttcc aagatgagca aggtgaagat
    26701 acagatcgaa tgattcgtga agttgtaaca gacgaagaaa ttggtgatat tgtcagccaa
    26761 tggacaggca taccagtttc aaaattagtt gaaacagaac gtgaaaaatt acttcactta
    26821 agtgacatct tgcataaacg tgttgtaggt caagataaag cggttgacct ggtttcagat
    26881 gcagtagtta gagcaagagc aggtattaaa gatccaaaca gacctattgg tagtttctta
    26941 ttcctaggtc caactggagt aggtaaaact gaattagcta aatcattagc tgcatcatta
    27001 tttgattctg aaaaacatat gattcgtatt gatatgagtg aatatatgga aaaacatgca
    27061 gtatcaagat tgataggggc acctccagga tatattggac atgatgaagg gggtcaatta
    27121 actgaagcgg ttcgtcgtaa tccatactca gttattttat tagatgaggt tgaaaaagcg
    27181 catactgacg tctttaatgt attattgcaa attttagatg aaggccgttt aactgattct
    27241 aaaggacgta gcgttgattt taaaaatact attattatta tgacaagtaa tattggatct
    27301 caagttttat tagaaaacgt aaaagagact ggtgaaatta cagaatcaac agaaaaagct
    27361 gttatgacaa gtttaaatgc atatttcaaa ccagaaattt tgaatcgtat ggatgatatc
    27421 gtattattta aaccattatc tattgatgac atgagtatga ttgtagataa aatcttaacg
    27481 caattaaata taagattatt agaacaacga atctcaattg aagtttctga tgatgctaaa
    27541 gcttggctag gtcaagaagc ttatgaacct caatacggtg caagaccatt aaaacgtttt
    27601 gtacaacgcc aaattgaaac accattagca cgtatgatga ttaaagaggg attcccagaa
    27661 ggtacaacga ttaaagttaa tttaaattca gacaataact taacgtttaa tgttgaaaaa
    27721 attcatgaat aaaattgaac caagaatgtg atgattaatc gcattcttgg ttttttagat
    27781 ttgaaattta ttataattgt tcattggcaa tatacttttt aaatgattta ataaacacca
    27841 atatttctgg gctttctttt ttgctgtata tatatgtaaa tgaaataggt gcttgtaaaa
    27901 ttttcgtgtt aataaccgaa atattgtaat cactatttgt tgttatataa agaggcagga
    27961 atgatatacc ttgattcatt tcgattaatt taattgaagt atgcacatca ttgatagata
    28021 gaaattgtgc tttttcataa atatttaaaa tattattttt aagtgatgac caatattctg
    28081 gatggttatc acttattatt ttgtattttt caaataaaga tgcctcagtt agaagatgat
    28141 tattctcttt attgggagca atcaatacaa ttttaccttc gcatactttt tcagaatgaa
    28201 cttctcttag tttaggttga tttctgctaa tcccgatgtc atacgtatga ttattaatat
    28261 ctttttcaat attttcattt ttgacatgaa gagaaacatc gataaaagga tgctcgttaa
    28321 agaaagattt taaaaatttg ggcataatga atgtcgcgat atatgaagac acgacaacat
    28381 ttaatttcga ttgaaacatc gtttttttaa gttggatatg tttgatgcca ctttcatatt
    28441 gttcaataaa actttgcgca attggaagaa atgtatgacc atcttcagtc aagataattt
    28501 gatttttata agtttcaaat agtttcacat tgagatgctg ttctaaattt ttaatttgct
    28561 tatgtataga aggtatagtg agattaattt cttcactagc taatcgatag tttaacgtct
    28621 tcgctaaaat gacaaatgta tagtaccaat ctaaattcat gaacttacct ctttcaaaaa
    28681 agttaataat tcattagata atatttaatt tttattgcat aaaaataata ttaatattat
    28741 atcagaggtt atcgaggggg tgaagtaata tgattcgaat tcaagataat acaataaggg
    28801 atggtatgca acaaagtaat gttcgaaaaa gtctaattat aaaaaaagaa gtattgaaac
    28861 aaattaacaa gttaaatata aattctgttg aagtaggcat gtgtacaact atcgaggatg
    28921 aatttaatat tcatcaattc agagacattt taagtcctga aaaagaatta gtagtattga
    28981 ctaggcttaa tgaaaaagaa ataaaaaaaa tagtcaaatt gaaaattcat aatttagtgg
    29041 taaaaatact attgccaata tctgacttgc atataaaaga aaagcttaat ttttcaaata
    29101 aatattatat tcagaaaatc aaagactgct tggatatatt aaagaaagat aaaaaaggag
    29161 tagatatttg ttttgaagat ggaacaagga cttctagaga aaaattgaaa gaatacatgg
    29221 aaattatttc aaaatatcaa gttagaacag ttacatttgc ggacactgta ggatgttcga
    29281 caccattaga atacggagat atttttaatt actttgtaaa aaaatattct aacataattt
    29341 tttctgctca ttgtcataac gatctagggt tggctactgc aaatacatta gctgcaattt
    29401 taaatggtgc aaagcaaata gaaactacat ttttgagaat tggtgagaga gcgggtaatg
    29461 ctcctattga ggaaataatt actattttga caaaaaaaca aatagaaagt acggaattca
    29521 ctttacccga cgtatataaa actagtatta atatttctaa aattctcgat tttcaaatat
    29581 cagaaaacaa acctataatt ggtgaaaata tatttaaaca tgaatcagga attcatcaag
    29641 atggtactaa aaaaaatata a
//
LOCUS       DALAAR010000033        28546 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000033 DALAAR010000000
VERSION     DALAAR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.033"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(220..1218)
                     /gene="sirC"
                     /locus_tag="O2458_001435"
     CDS             complement(220..1218)
                     /gene="sirC"
                     /locus_tag="O2458_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCZ3887302.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1215..2210)
                     /gene="sirB"
                     /locus_tag="O2458_001436"
     CDS             complement(1215..2210)
                     /gene="sirB"
                     /locus_tag="O2458_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCZ3887303.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2226..3218)
                     /gene="sirA"
                     /locus_tag="O2458_001437"
     CDS             complement(2226..3218)
                     /gene="sirA"
                     /locus_tag="O2458_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCZ3887304.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3449..4429
                     /gene="sbnA"
                     /locus_tag="O2458_001438"
     CDS             3449..4429
                     /gene="sbnA"
                     /locus_tag="O2458_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCZ3887305.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4426..5436
                     /gene="sbnB"
                     /locus_tag="O2458_001439"
     CDS             4426..5436
                     /gene="sbnB"
                     /locus_tag="O2458_001439"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCZ3887306.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5457..7211
                     /gene="sbnC"
                     /locus_tag="O2458_001440"
     CDS             5457..7211
                     /gene="sbnC"
                     /locus_tag="O2458_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCZ3887307.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7204..8460
                     /gene="sbnD"
                     /locus_tag="O2458_001441"
     CDS             7204..8460
                     /gene="sbnD"
                     /locus_tag="O2458_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCZ3887308.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8450..10186
                     /gene="sbnE"
                     /locus_tag="O2458_001442"
     CDS             8450..10186
                     /gene="sbnE"
                     /locus_tag="O2458_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCZ3887309.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10095..11945
                     /gene="sbnF"
                     /locus_tag="O2458_001443"
     CDS             10095..11945
                     /gene="sbnF"
                     /locus_tag="O2458_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCZ3887310.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11920..12696
                     /gene="sbnG"
                     /locus_tag="O2458_001444"
     CDS             11920..12696
                     /gene="sbnG"
                     /locus_tag="O2458_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCZ3887311.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12696..13898
                     /gene="sbnH"
                     /locus_tag="O2458_001445"
     CDS             12696..13898
                     /gene="sbnH"
                     /locus_tag="O2458_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCZ3887312.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13902..14666
                     /gene="sbnI"
                     /locus_tag="O2458_001446"
     CDS             13902..14666
                     /gene="sbnI"
                     /locus_tag="O2458_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCZ3887313.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14862..15269
                     /locus_tag="O2458_001447"
     CDS             14862..15269
                     /locus_tag="O2458_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887314.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15319..15945
                     /locus_tag="O2458_001448"
     CDS             15319..15945
                     /locus_tag="O2458_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887315.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16157..16933
                     /locus_tag="O2458_001449"
     CDS             16157..16933
                     /locus_tag="O2458_001449"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCZ3887316.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17077..17199
                     /locus_tag="O2458_001450"
     CDS             17077..17199
                     /locus_tag="O2458_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887317.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17269..18249
                     /locus_tag="O2458_001451"
     CDS             17269..18249
                     /locus_tag="O2458_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ3887318.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18212..18904
                     /locus_tag="O2458_001452"
     CDS             18212..18904
                     /locus_tag="O2458_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCZ3887319.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19114..20280
                     /locus_tag="O2458_001453"
     CDS             19114..20280
                     /locus_tag="O2458_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCZ3887320.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20261..21499
                     /locus_tag="O2458_001454"
     CDS             20261..21499
                     /locus_tag="O2458_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ3887321.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21489..22919
                     /locus_tag="O2458_001455"
     CDS             21489..22919
                     /locus_tag="O2458_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCZ3887322.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            23187..23786
                     /locus_tag="O2458_001456"
     CDS             23187..23786
                     /locus_tag="O2458_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ3887323.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            24154..24879
                     /gene="sasD"
                     /locus_tag="O2458_001457"
     CDS             24154..24879
                     /gene="sasD"
                     /locus_tag="O2458_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCZ3887324.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(25071..25826)
                     /locus_tag="O2458_001458"
     CDS             complement(25071..25826)
                     /locus_tag="O2458_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3887325.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            26062..26769
                     /gene="deoD"
                     /locus_tag="O2458_001459"
     CDS             26062..26769
                     /gene="deoD"
                     /locus_tag="O2458_001459"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ3887326.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            26776..28128
                     /gene="tet(38)"
                     /locus_tag="O2458_001460"
     CDS             26776..28128
                     /gene="tet(38)"
                     /locus_tag="O2458_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ3887327.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            28209..>28546
                     /gene="deoC"
                     /locus_tag="O2458_001461"
     CDS             28209..>28546
                     /gene="deoC"
                     /locus_tag="O2458_001461"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ3887328.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT         9587 a         4406 c         5444 g         9109 t
ORIGIN      
        1 aagaaatagt tatcgatatt tgaaaaatcg aagaatattg attgatatta tagaatatga
       61 gtcggtgtta tttgtatata aagtattaat atgtaattaa gaaagtcgac gattgttaac
      121 aataacaaca actatatgta taaaaaaacc tcgcaacggt tagttaactg aatcatttag
      181 ctaacttcgt tgtgaggtca ttttgtttta ataatatcgt tataactttt tcacggttaa
      241 taataagtat atgaagaatg gggcaccaaa agcagcaata aatacacctg ctggcacttc
      301 tttaggcaag aataaggtac gcccaattaa gtctgcaata acaattgata tggcaccaat
      361 cattgctgac attagtaact ttttagcata acttccgcga acgattgttt tcgcgatatg
      421 tggtgcgatt aaaccgacaa acccaatgtt acctactaaa ctgattgcca tagatacgag
      481 tatagtagaa gtgattaatt ggattagttt catacgttgt acatgtaagc ctaagccaat
      541 cgctacaggg tcatcaagta tagatatttt catttttggt ataacaagaa ataacaacgg
      601 cacaacagct aaaataacca tacccaaaat gattgtatct ttaaacgtag caccgtaaag
      661 acttccgact agccatgtat aagctttggc agcagataat tgcttcgttg taatgagtaa
      721 tccttggaca agcgcaataa acaacgtttg catcgaaata ccgatgatta tgagtgttgt
      781 cgggcgtatt tgtcctttcg tttgaaacac taatagtatc atcattgcaa ctgcgccacc
      841 taatactgca aatagtggaa gtaaatgtat tgttaaatgg ctgaaaaatg caataaagac
      901 aacagcactt aagctagcac cacctgtgat accgataata tcaggtgagg caattggatt
      961 ttttaataca ttttgcaaca ttaaaccact cattcctagt gcggcacctg ctaaaatcgc
     1021 aagtgtaatg cgaggtaagc gtaatacttc taaagtgaat tgatccatac tgtcatttgg
     1081 atttataaag tacatcagta cgcgttgtaa tggtataaag cttgaaccaa tcatcatact
     1141 taccactgaa acgatggcta aaaagattaa cgcgaagatg agatggtaat tgtctttttt
     1201 attaatcttt tcggtcataa gcgttgacgt cctttcttca taatatagat taagacaata
     1261 gcgccaatga cagcggtaac gacaccgata ggcaactcta gtggcttaat tattatacga
     1321 gcaacaatgt ctgaaatgat cattaggatt gctccagcta atgcagtaaa aggaattaaa
     1381 tacttatagt ttggtggtaa taatcgtttg ctaatattcg gtacgataag acccacaaag
     1441 acgattgatc cagctacggc taccgaaata ccggctaaca tactgatgag cataataatc
     1501 atccatttga ttaattttat gttttgaccg aggccggttg caatgtcgtc acttgtcatc
     1561 aagatgttga tgtgtgcagc catgctaaat gcaattaaaa taagtatcaa tacaagcgga
     1621 ataatccatg ggatatccca aatattacgt aatgaaacgg agccacttaa ccaaaataat
     1681 aggccttgta agtctgtttc gttcataata agtatgcctt gagtaaaggc tgtaaatagc
     1741 atcgcaatcg cagcacctgc caaaatgaca cggtgaggtg agaatagtgt ttgtctaaac
     1801 atacctagtg caacaactaa tacagtaaca acaatagccc ccaaaaatgc aataactaca
     1861 atcattttaa aagattgaat ttggataaat gtaatactaa aaatgacaaa aaatactgcg
     1921 cctgcattga caccgaaaag ccctggtgag gctattgggt ttcgtgtaag tgcttgcatc
     1981 aacaaacctg agacagcaag ggcagcacca gtcaataacg caatgattgt tctcgacgcc
     2041 cgtgcaccag tgacaacatc atgtaaatcg ttttcactat caaagttgaa taacgcctgt
     2101 atcaccgtac ctggtgacac aagcgtattt ccaatcatta aacttaagat agctactatt
     2161 gcaagacata aaccagcaat aacgatttgg tattttggtt taagtagcat cgtaaaactc
     2221 cttaattatt ttgattgttt ttcaatattt aacttttcat ataaatcgtc aataagtttt
     2281 aatgaagatt tatatccgcc agctaagttc caagtgattt catctaaatc atcagatact
     2341 tggttgtttt taactgcgtc taaatttttc cactctttac ttgaagtcca ttcgctttca
     2401 gtctttttaa ctaatgcagc atctttcgca tttggatctg attttactac aaaaatatga
     2461 tcagcgttca ttaatggaat gctttcttta gatgtaagtt ggataatatc tttaccatta
     2521 tcaacttgtt tttgtaagtc tttattacgt ttgaatccta aatcatttaa gatttcacca
     2581 gcatatccac cagcataaat tcttgtatga tcagcacgga agttaacaac tgaagctttc
     2641 aatggccatg catctttata ctttgctttt gcatcttttt ggaatgcagc tactttatca
     2701 tcgtactttt taagtaaatc ttcagcttct ttttctttcc ctaaagcttt ccccattaac
     2761 ttagttgtat ctttgaattt gaaaactgta tcagtagaaa ctgttggtgc gattttagat
     2821 aattgatcgt aaactttttc atttctaact tttgacgcga caattaagtc cggttttaat
     2881 ttagagattt cctctaagtt aggtgcaggt tcttgaccta caatcttagt atcttttaaa
     2941 tcatttttta tgtattcgaa tttcggtttt tgtgtccatg attctacagc acctacaggt
     3001 ttaacaccta aagatacagc gacgtcagtg gcaccttgat atagcgtaac aacacgcttt
     3061 ggtttccctt taatttcagt tgtacccatt gcatgtttaa ttgaagttgt ttccttatct
     3121 ttgttatcag atgattgttt atttgaattc ccactacatc ctgctaaaac aagtaggaaa
     3181 gcaagcgtaa caacaagcat tttaattact ttattcattg actaattagc ctccttcgtg
     3241 atgtatgaca atgagaatca ttatcacgat ttagtatgaa ttaaattttt tcctaagtca
     3301 ataaaatatt tatgatttac atgcaactta taattatttg acatataaat gcataaaaaa
     3361 tataatccta attacttgat agtgagaatc attatcaatt aggtaacaca caatattata
     3421 gaattttaaa tttgaggagg aagcgctttt gattgaaaaa agtcaagcat gtcacgattc
     3481 attgttagat tctgtagggc aaacacctat ggttcaactt catcaactat ttccgaaaca
     3541 tgaagtgttt gcaaagttag agtatatgaa tcctggaggc agcatgaaag atcgacctgc
     3601 caagtacatc attgaacatg gtattaaaca tggtttaatc actgagaata cacatttaat
     3661 tgaaagtact tctggtaatt taggcattgc gttggcaatg atagctaaaa tcaagggatt
     3721 aaaactcacg tgtgttgttg atcctaaaat atcaccaaca aatttgaaaa ttattaaaag
     3781 ttatggtgcc aatgtagaaa tggttgaaga acctgatgca catgggggtt atttaatgac
     3841 tcgtattgca aaggtgcaag aactgttagc cactattgac gatgcatatt ggattaatca
     3901 atatgcgaat gagttaaatt ggcaatccca ttatcatggt gcaggcacag agattgttga
     3961 aacaattaag caacctatag attattttgt cgcgccagtc agcacgacag gtagcattat
     4021 gggtatgagt agaaaaataa aagaagtgca tccaaacgca caaattgttg ctgttgatgc
     4081 gaaagggtca gtcatttttg gtgacaaacc tattaataga gaattacctg gtatcggtgc
     4141 tagtcgtgta cccgaaatat tgaatagatc agaaattaat caagtgatcc atgtagatga
     4201 ttatcaatct gctttgggct gtcgaaaact gattgattat gaaggcatat ttgccggagg
     4261 ttcaacaggt tcgattattg cagcgattga gcagttgata acgtcaattg aagaaggtgc
     4321 aacaattgtc acgattttac cagatcgagg cgatcgttac ttagatttag tttattcaga
     4381 tacatggtta gaaaaaatga aatcaagaca aggagttaaa tcagaatgaa tagagagatg
     4441 ttgtatttaa atagatcaga tattgaacaa gcgggaggta atcattcaca agtttatgtg
     4501 gacgcattaa cagaagcatt aacagcccat gcgcacaatg attttgtaca accgcttaag
     4561 ccgtatttaa gacaggatcc tgaaaatgga cacatcgcag atcgaattat tgcaatgcca
     4621 agtcatatcg gtggtgaaca cgcaatttca ggtattaagt ggataggtag taagcacgac
     4681 aatccatcga aacgtaatat ggagcgtgca agtggcgtca ttattttgaa tgatccagaa
     4741 acgaattatc caattgcagt tatggaagca agtttaatta gtagtatgcg tactgcagca
     4801 gtttcagtga ttgcagcaaa gcatttggct aaaaaaggat ttaaagactt aacaatcatt
     4861 ggatgcgggc taatcggaga caagcaatta caaagtatgt tagagcaatt cgatcatatt
     4921 gaacgcgtgt ttgtttacga tcaattctct gaagcatgtg cacgctttgt tgatagatgg
     4981 caacaacagc gtccggaaat taattttatt gcgacagaaa atgctaaaga agcagtatca
     5041 aatggtgaag tagtcattac atgtaccgta acggatcaac catacattga atatgattgg
     5101 ttacaaaagg gtgcatttat tagcaacatt tctatcatgg atgtgcataa agaagtcttt
     5161 attaaagctg acaaagtcgt agtagatgac tggtcacaat gtaatcgaga aaagaaaact
     5221 attaaccaat tggtgttaga aggtaaattc agcaaagaag ctcttcatgc tgaactagga
     5281 caacttgtga caggtgacat accaggacgt gaagacgatg atgagatcat attacttaat
     5341 ccgatgggta tggctatcga agatatttca agtgcttatt ttatttatca acaggcacaa
     5401 caacaaaata ttgggacaac attgaaccta tattaagaat gcgaggtgtc tgaacattgc
     5461 agaatcatac agcagtcaat acagcacaag cgataatatt aagagattta gttgatgcat
     5521 tattatttga agatatagcc ggaattgtat cgaatagtga gattactaaa gaaaacggac
     5581 aaacgctttt gatatacgaa cgtgaaacac aacaaataaa gatacctgtt tattttagtg
     5641 ctttaaatat gtttcgttac gaaagttcac aaccaattac gatagaggga agggtgtcta
     5701 agcaaccttt aacggcagct gaattttggc aaacaattgc taatatgaat tgtgatctaa
     5761 gtcatgaatg ggaagtggct cgcgttgaag aaggactgac tactgctgcc acacagcttg
     5821 ctaaacaatt atcagaatta gatttagcgt cacatccttt tgtgatgtca gagcagtttg
     5881 caagtttaaa agatcgtcca tttcatccat tagctaaaga aaaaagagga ttaagagaag
     5941 cggattatca agtgtatcaa gctgaattaa atcaatcatt tcctttaatg gttgcagcag
     6001 ttaaaaagac acatatgatt catggcgata ctgcaaatat cgatgaatta gaaaatttga
     6061 cagtacctat aaaagaacaa gcgacagaca tgttaaatga tcaagggtta tcaatagatg
     6121 actatgtact atttccggta catccttggc aatatcagca tattctgccg aacgtctttg
     6181 cgaaagagat tagtgaaaag ttggttgtac tattaccgtt aaaatttgga gattatctgt
     6241 cgtcttcaag tatgcgttca ttaattgata ttggcgcacc gtataaccat gtcaaagtac
     6301 catttgcaat gcagtcatta ggcgcattaa ggctaacgcc tacgcgttac atgaaaaacg
     6361 gagaacaagc agaacaatta ttacgtcagc ttatagaaaa agatgaagca ctagctaagt
     6421 atgtcatggt ttgtgatgaa acagcttggt ggtcatatat gggtcaagat aatgatattt
     6481 tcaaagatca attaggtcat ctaactgttc agctaagaaa gtatcccgaa gtgctagcca
     6541 aaaatgatac gcaacagcta gtgtcaatgg cagcactcgc ggcaaatgat cgcactttat
     6601 atcaaatgat ttgtggaaaa gataatattt ctaaaaatga tgtcatgacg ttatttgaag
     6661 atatcgcgca agtcttttta aaggtaacac tatcatttat gcaatacggc gcattaccag
     6721 agttgcatgg tcaaaatata ttgttgtcat ttgaagatgg acgtgtacaa aaatgcgtgt
     6781 tacgtgatca tgatactgtc agaatttata aaccatggct aacagcacat cagctttcat
     6841 tgccgaagta tgtcgtcaga gaagatacac ctaatacgct aattaatgag gatttggaaa
     6901 cattctttgc ttattttcaa acattagctg tatcggtaaa tctatatgcc attattgatg
     6961 caattcaaga tttatttggt gtaagtgagc atgaacttat gtcgttgtta aaacaaattt
     7021 taaaaaatga agtggcaact atttcctggg ttacaactga tcagctagct gtcagacaca
     7081 ttttatttga taaacagacg tggccattca aacaaatttt attaccattg ctatatcaac
     7141 gtgatagtgg tggaggtagt atgccttcag gtttaactac cgtaccaaat ccaatggtga
     7201 catatgatta atcagtctat atggcgcagt aactttcgca ttttatggct cagtcagttt
     7261 atagcgattg ctggactgac agtacttgtg ccattattgc caatttatat ggcatcacta
     7321 caaaatctat cagtcgtaga aatacagttg tggagtggta tagcgattgc tgctccagct
     7381 gtaacgacga tgatagcttc gccgatatgg gggaagctag gtgataagat cagccgaaaa
     7441 tggatggtgt taagagcgtt acttggtttg gcggtatgct tatttttaat ggcattgtgt
     7501 acgacaccat tacagtttgt acttgtgagg ttattgcagg gactatttgg tggtgttgtt
     7561 gatgcatcaa gtgcgtttgc gagtgcagag gcgccagctg aagatcgtgg aaaggtatta
     7621 ggaagactgc aaagttcagt cagcgcaggg tctcttgtgg ggccattaat tggcggtgtt
     7681 acagcttcga tattaggttt tagtgcgtta ctgatgagta ttgccgttat tacttttatt
     7741 gtctgtattt tcggtgcatt aaaattgatt gaaacgacac atatgccaaa atcacaaaca
     7801 ccaaatatta ataaaggtat tcgccgttca tttcaatgtc tattatgcac acaacaaaca
     7861 tgtcgattta ttatcgttgg cgttttagca aactttgcta tgtatggcat gctaactgca
     7921 ttatcaccac ttgcttcatc agtgaatcat acagcgatag atgaccgtag tgtgattgga
     7981 tttttacagt ccgcattttg gacggcttcg atattaagcg cgcctttatg gggacgcttt
     8041 aatgataaat catatgttaa atcagtatat atatttgcca cgattgcatg tggttgtagt
     8101 gcgatactgc aaggtttagc gacgaatata gagtttttaa tggctgcaag aatacttcaa
     8161 ggattaacat atagtgcatt gattcaaagt gtcatgtttg ttgtcgtgaa tgcgtgtcat
     8221 caacaactta aaggcacatt tgttggaacg acgaacagta tgttagttgt tggtcaaatt
     8281 attggcagtc ttagtggcgc tgccattaca agttatacta caccagctac tacgtttatc
     8341 gttatgggcg tagtatttgc agtaagtagt ttatttttaa tttgttcaac catcactaat
     8401 caaatcaacg atcacacatt aatgaaatta tgggagttga aacaaaaaag tgcaaaataa
     8461 agaattaata caacatgcag cgtatgcggc tatcgaacgc attttaaatg aatattttag
     8521 agaagaaaat ttatatcaag taccacctca aaatcatcaa tggtctatac aattatcaga
     8581 gctcgaaact ttaacgggtg aatttcgcta ttggtctgcg atggggcatc atatgtatca
     8641 tccagaggta tggcttatcg atggaaaaag taaaaaaata acaacttata aagaagcaat
     8701 tgcgcgtatt ttgcaacata tggctcaaag tgcagataat caaacggcag tgcaacaaca
     8761 tatggcgcaa attatgtctg acatcgataa tagcattcat cgcacggcac gttatttgca
     8821 aagtaacaca atagactacg tagaggatcg ttatatcgtt tcagaacaat ctttatactt
     8881 aggtcatcca tttcatccga ctcctaagag tgcaagtggg ttttcagaag cagatttaga
     8941 gaaatatgca cccgaatgtc atacatcatt ccaattgcat tatttagctg tgcatcaaga
     9001 tgttctgctc acgcgctatg tagaaggtaa agaagatcag gttgagaaag tgttgtatca
     9061 attagcagac atagatatat cagagatacc caaagatttt attttattac caacacatcc
     9121 ttatcaaatc aatgtgttgc gacagcatcc acagtatatg caatatagtg aacaaggttt
     9181 aataaaagac cttggcgttt ccggtgattc agtgtacccg acgtcttcgg ttagaactgt
     9241 attttcaaaa gcattaaaca tttatttaaa attaccgata cacgttaaaa tcactaattt
     9301 tatacgtacg aatgaccttg aacagattga acggacaatt gatgccgcgc aagttatcgc
     9361 atcagtcaaa gatgaggttg aaacacccca ttttaaattg atgtttgaag aaggatatcg
     9421 tgcattgtta ccgaatccat tagggcaaac agttgaacct gaaatggatt tattaacaaa
     9481 tagtgccatg attgttcgtg aagggatacc gaattaccat gctgataaag atattcatgt
     9541 attggcgtca ttatttgaaa cgatgcctga ttcaccgatg tctaagttat cacaagtgat
     9601 tgagcaaagt ggtttagcac cagaagcatg gcttgaatgt tatttgaatc gtacattatt
     9661 gccgatatta aagctgttta gtaacacagg cattagtcta gaagcacatg tacaaaatac
     9721 attaattgaa ttaaaagatg gcatacccga cgtatgcttt gtcagagatc ttgaaggcat
     9781 ttgtctatct agaacgattg ctactgaaaa acagcttgtg ccaaatgttg tggcagcatc
     9841 aagccctgtt gtatatgcac atgatgaagc atggcatcgt cttaaatatt acgttgtagt
     9901 aaatcactta ggacatttag tatcaactat tggtaaagcg actagaaatg aagttgtgtt
     9961 atggcaactt gtagcgcatc gtcttatgac ttggaaaaaa gaatacgcga ataacgcagt
    10021 atttgttgac tgtgtagaag atttatatca aacgccgacc attgcggcta aagcgaattt
    10081 gatgagtaaa ttgaatgatt gtggtgcaaa ccctatttat acacatatac caaatccaat
    10141 ttgtcataac aaggaggtat cgtattgtga atcaaacaat tcttaatcgt gtaaagacta
    10201 gagtgatgca ccaactggta tcatcactta tttatgagaa tattgttgtg tataaagcgt
    10261 catatcaaga cggtgtcggt cattttacaa tagaaggaca tgattcagag tatcgtttta
    10321 ctgctgaaaa gacacatagc tttgatcgta tacgtatcac atcaccaatt gagcgtgtcg
    10381 taggagatga ggcagataca acaacagact atacacaatt attgagagag gttgtattta
    10441 catttcctaa aaatgatgaa aagctagaac aatttattgt cgagttatta cagacagaat
    10501 taaaagatac gcaaagtatg cagtatcgag aatcaaaccc accagcaaca cctgagacat
    10561 ttaacgacta tgaattttat gcgatggaag ggcatcagta tcatccaagt tacaaatcac
    10621 gtttaggatt tacgttgagt gataatttga aatttggtcc tgattttgta ccaaacgtta
    10681 aactgcagtg gttagctatc gacaaagata aagtagaaac gacggtatca agaaatgttg
    10741 tagttaacga aatgttacgt caacaagttg gcgataagac ttatgaacat tttgtacagc
    10801 agattgaagc atctggcaaa catgtaaatg atgttgagat gatacctgta cacccatggc
    10861 agtttgaaca tgtcatccaa gttgatttgg ctgaagaaag gcttaatggc acagtactat
    10921 ggttagggga aagtgatgag ctatatcacc ctcaacaatc gattcgtacg atgtcgccaa
    10981 tagacacgac aaaatattat ttaaaggtac caataagtat aacgaacact tcaacgaaac
    11041 gagtgttggc gcctcataca attgaaaatg cagcgcaaat tacggattgg ttaaagcaga
    11101 tacagcaaca agatatgtat ttaaaagatg aattaaagac agtttttcta ggggaagtct
    11161 taggacagtc ttatttaaat acacaacttt cgccttataa acaaactcaa gtttatggtg
    11221 cgttaggtgt tatatggcgt gaaaatatat atcatatgtt aatcgatgaa gaggatgcga
    11281 taccatttaa tgcactttat gcaagtgata aggatggtgt accattcatt gaaaattgga
    11341 ttaaacaata tggttctgaa gcttggacaa agcaattttt agctgtagcg attcgtccaa
    11401 tgattcatat gctttattat cacggtattg cctttgaatc gcatgcacaa aatatgatgc
    11461 tcattcatga aaatggttgg cctacacgta ttgccttaaa agatttccat gatggtgttc
    11521 gttttaagcg tgagcattta agtgaagctg cttcacacct gacattaaag ccaatgccag
    11581 aagcacataa aaaagtgaat agtaattcat ttattgaaac agatgacgaa cgtttagtac
    11641 gcgacttttt acatgatgca tttttcttta ttaatatcgc cgaaatcatc ttatttattg
    11701 aaaagcaata tggtatcgat gaggagctgc aatggcaatg ggttaaaggc atcatcgagg
    11761 cgtatcaaga agcatttcca gagttgaata actatcaaca tttcgatttg tttgaaccta
    11821 cgattcaagt tgaaaagtta acgacacgtc gattattaag tgactccgag ttaagaattc
    11881 atcatgttac aaatccatta ggtgtaggag gtatcaatga tgcaacaact atctctgaaa
    11941 catagattaa acaatggtga ttcagtttat ggcattttta attctatacc ggacccattg
    12001 atgatcgagg ttatcgcagc aagcgggtat gactttgttg tgattgatac agaacacgtg
    12061 gcgattaatg atgagacact agcgcattta attcgtgcag ctgaagcagc gcatattata
    12121 ccaattgtac gtgtcactgc agtgatagat agagatatca ttaaagtgtt ggatatgggt
    12181 gcgagaggta ttattgtgcc acacgttaaa gatcgtgaga cagttgagca tattgtgaaa
    12241 ttaagtcgtt attacccgca aggattaaga agtttgaatg gtggtcgcat ggcaagattt
    12301 ggacgtacac cattacttga tgcaatggag atggctaatg agcatattat ggtgattgcc
    12361 atgatagaag atgttgaagg ggttatggcc attgacgata tagcacaagt cgaaggttta
    12421 gacatgatag tcgaaggtgc cgcagattta tcgcagtcac ttggcatacc atggcaaacg
    12481 cgtgatgatc aagtaacatc acatgttcaa catatttttg aagttgtgaa tgcacatggt
    12541 aaacattttt gtgcattacc acgtgaagat gaagatattg caaaatggca ggcacaaggt
    12601 gtacaaacat ttattttagg tgatgatcgc ggaaaaatat atcgccattt aagtgcatct
    12661 ctagcgacgt ctaaacagaa aggggatgaa ggctaatgcg tatagttcaa cctgttattg
    12721 aacaattaaa agcacaatct catccagttt gtcattatat ctatgattta gtcggactgg
    12781 aacatcattt gcaacatatt acatcgtcat tgccgagtaa ttgtcaaatg tactatgcaa
    12841 tgaaagcaaa tagtgaacga aaaatcctag atacaattag tcagtatgtt gaaggattcg
    12901 aagttgcatc tcaaggtgaa atagcaaaag gtcttgcttt taaaccagca aatcatatta
    12961 tttttggtgg ccctggtaag acagacgagg aactaagata tgcagtaagt gaaggtgttc
    13021 agcgtattca tgttgaaagt atgcatgaat tacaacggct aaatgccatc ttagaagatg
    13081 aagataagac acaacacatt ttattgcgtg ttaatttagc aggaccattt cccaatgcaa
    13141 cgttgcatat ggcaggacgc ccaacacaat ttggtatttc tgaagacgaa gttgatgatg
    13201 tcattgaagc tgcgctcgca atgccaaaga ttcatctaga tggatttcat tttcattcta
    13261 tttctaacaa tttagactcg aatttacatg tcgatgtagt gaaactttat tttaaaaaag
    13321 caaaggcatg gtctgaaaaa catcgatttc cactcaaaca tatcaatctt ggtggtggca
    13381 taggcgttaa ctatgcagat ttaactaacc aatttgaatg ggataatttt gtagaacgtt
    13441 ttaaaacact tatcgttgag caagaaatgg aagatgtgac attgaacttt gaatgtgggc
    13501 gctttattgt ggcacatatt ggttactatg tgacagaagt gctagacatt aagaaagtac
    13561 atggtgcttg gtatgccata ttaagaggag gtacgcaaca atttagactg ccggtatctt
    13621 ggcaacataa ccatcctttt gacatttatc gctataagga caatccatat tcatttgaaa
    13681 aagtttcaat ttcgagacag gacacaacgt tagtcggtca attatgtaca ccgaaagatg
    13741 tctttgctag agaagtacag atagacgcaa tcagtacagg cgacgttatt gttttcaaat
    13801 atgcaggtgc atacggatgg tctatttcac atcacgattt cttaagccat ccacatcctg
    13861 aatttattta tttaacgcaa acaaaggagg atgaataact attgaatcat attcatgaac
    13921 atttaaaatt ggtaccagta gataagattg atcttcacga aacattcgaa cctttaagat
    13981 tggaaaaaac gaaaagtagt attgaagcag atgattttat acgtcatcct attttagtga
    14041 cagcgatgca acatggtaga tatatggtta tagatggtgt gcatcggtat acaagtttga
    14101 aagcgttagg atgtaagaaa gttccagtgc aagaaatcca tgaaacacaa tattcaatta
    14161 gtacatggca acataaagtt ccatttggtg tgtggtggga aacgttacaa caagaacatc
    14221 gcttgccatg gactactgag acaagacaag aagcgccatt tattacaatg tgtcatggtg
    14281 atacagaaca atatttgtat acaaaagatt taggcgaagc acattttcaa gtatgggaaa
    14341 aggttgtcgc aagttatagt ggttgttgtt ctgtagagag aattgcacaa ggtacatatc
    14401 cttgtctttc tcaacaagat gtactcatga agtatcagcc attgagttat aaggaaattg
    14461 aagcggttgt tcataaaggg gaaactgtgc cagcaggtgt gacacgcttt aatatttcag
    14521 gacgatgtct taatcttcaa gtaccactgg cattacttaa acaagatgat gatgttgaac
    14581 aactgcgcaa ttggaagcag tttttagcag ataagtttgc caatatgaga tgctatactg
    14641 aaaaagtata cttggtggag caatagtttt actgtgatgt tgagggaaat atgatgattt
    14701 agcgtattga tagcgaaaat ataataaaac aatatagtgt ggagaacttt tgatatttta
    14761 taaatattga agttctccat ttttgtattt tgcatataaa aattaaataa aataaggtat
    14821 attaaggtaa agtataaatt ttaaataaat ggggaatgag tatgagctca attataggaa
    14881 aaatagcaat ttggataggc atcgtagctc aaatatattt tagtgtcgtt tttgttagga
    14941 tgatatctat taatattgct ggaggatctg attacgaaac aattttttta ttaggattaa
    15001 tattggctct tttcactgtt ttaccaacca tctttactgc gatttatatg gaaagttact
    15061 ctgtaatcgg aggtgcactt tttattgttt atgctattat tgcactgtgt ttatataatt
    15121 tcctttcgtc aattttatgg ctgattggtg gtattttgct gatttggaat aaatactcaa
    15181 aagatgaatc gacagacgaa aatgaaaaag ttgatattga aagtacagag aatcaatttg
    15241 aatctaaaga taaaatcact aaagaataaa gagaatattt aaggtaaagt ataaatttta
    15301 aataaatggg gaatagacat ggaaaaaaat gtagaaaaat cattcataaa gataggttta
    15361 tattttcaaa tagcttatat agtactcatg gctataactt tatgtgggtt tgtaatttgc
    15421 tatggactaa ttttcggcct tttctattta ttatcaggta gcagagctga ttatttaata
    15481 gtaacaatag ttatatcggc aataatttct atatttgtaa ttatactttc aatcgtacct
    15541 gtcatcgtat tggcatctga cttatttaaa gaaaggattt caaaaggtgt catattaatt
    15601 gtattggcta ttatcgcttt agtattatgc aactttgtat ctgcaatact ctggtttgtt
    15661 tcagccatat ctattttagg tagaaaaaaa ttagtagctg cagcagatac taccactatt
    15721 caaaaaagta aagggaacgc aaatcaagca tcacataaag acacgtgtaa aaaggaactt
    15781 gatagtcaag acatgatgga acatcctgag gttaaaaatc ccacgactaa aaaccttgaa
    15841 ggatttaacg aagaaataca taaagatgaa gctacaacta aagttgtcag tgataacacg
    15901 gaaccgccta ttgaatcaaa agaccatgtc tcgaaaaaag attgatgaca aactaatcga
    15961 gagacttaaa aaaataatat tcaacataag aacttttaaa acgacattta aacgcattgc
    16021 caatcactaa tggtagtgcg tttaactata ccttaaatat ctgaatattt tgttaaatgg
    16081 agctaccttt gttgtactat tcaaatgaag aggagtaaaa tgtaattaaa ggaaagaaat
    16141 ttgaggagtg atctttatga caaacaacaa agtagcatta gtaactggcg gagcacaagg
    16201 gattggtttt aaaattgcag aacgtttagt ggaagatggt ttcaaagtag cagttgttga
    16261 tttcaatgaa gaaggggcaa aagcagctgc acttaaatta tcaagtgatg gtacaaaagc
    16321 tattgctatc aaagcagatg tatcaaaccg tgatgatgta tttaacgcag taagacaaac
    16381 tgccgcgcaa tttggcgatt tccatgtcat ggttaacaat gccggccttg gaccaacaac
    16441 accaatcgat acaattactg aagaacagtt taaaacagta tatggcgtga acgttgcagg
    16501 tgtgctatgg ggtattcaag ccgcacatga acaatttaaa aaattcaatc atggcggtaa
    16561 aattatcaat gcaacatctc aagcaggcgt tgagggtaac ccaggcttgt ctttatattg
    16621 cagtacaaaa ttcgcagtgc gaggtttaac acaagtagcc gcacaagatt tagcgtctga
    16681 aggtattact gtgaatgcat tcgcacctgg tatcgttcaa acaccaatga tggaaagtat
    16741 cgcagtggca acagccgaag aagcaggtaa acctgaagca tggggttggg aacaatttac
    16801 aagtcagatt gctttgggca gagtttctca accagaagat gtttcaaatg tagtgagctt
    16861 cttagctggt aaagactctg attacattac tggacaaaca attattgtag atggtggtat
    16921 gagattccgt taataatcat ccactaatga taaataaatc cttattgtta agtttaatca
    16981 cttagcagta aggatttttt agtgcactta gaagggagtg tattggtaga aaattaataa
    17041 gcgaagttct taagtgagtt atgatgtcac agtctaatgc atcagttgaa agcattatta
    17101 gtattaacac acccaagata ttataaaaca tcacaaaaac accactatct aatttatctc
    17161 aataaaaatt cacaaagtta tctcatttta tttttataaa taaaaaatat cgataaaaag
    17221 cttacaatac tttatgtttt tatgatatat ttttaatgta taaatgaggt ggaagatttg
    17281 gaaagagttt tgataactgg tggggctggt tttattgggt cgcatttagt agatgattta
    17341 caacaagatt atgatgttta tgttctagat aactatagaa caggtaaacg agaaaatatt
    17401 aaaagtttgg ctgacgatca tgtgtttgaa ttagatattc gtgaatatga tgcagttgaa
    17461 caaatcatga agacatatca atttgattat gttattcatt tagcagcatt agttagtgtt
    17521 gctgagtcgg ttgagaaacc tatcttatct caagaaataa acgtcgtagc aacattaaga
    17581 ttgttagaaa tcattaaaaa atataataat catataaaac gttttatctt tgcttcgtca
    17641 gcagctgttt atggtgatct tcctgatttg cctaaaagtg atcaatcatt aatcttacca
    17701 ttatcaccat atgcaataga taaatattac ggcgaacgga cgacattaaa ttattgttcg
    17761 ttatataaca taccaacagc ggttgttaaa ttttttaatg tatttgggcc aagacaggat
    17821 cctaagtcac aatattcagg tgtgatttca aagatgttcg attcatttga gcataacaag
    17881 ccatttacat tttttggtga cggactgcaa actagagatt ttgtatatgt atatgatgtt
    17941 gttcaatctg tacgcttaat tatggaacac aaagatgcaa ttggacacgg ttataacatt
    18001 ggtacaggca cttttactaa tttattagag gtttatcgta ttattggtga attatatgga
    18061 aaatcagtcg agcatgaatt taaagaagca cgaaaaggag atattaagca ttcttatgca
    18121 gatatttcta acttaaaggc attaggattt gttcctaaat atacagtaga aacaggttta
    18181 aaggattact ttaattttga ggtagataat attgaagaag ttacagctaa agaagtggaa
    18241 atgtcgtgaa aatgacattg aagctgtcca taataataag ggttatgcct atcaaagaaa
    18301 attagacaaa ctagaagaag tgagaaaaag ctattaccca attaaacgtg cgattgactt
    18361 aattttaagc attgttttat tatttttaac tttaccgatt atggttatat tcgccattgc
    18421 tatcgtcata gattcgccag gaaaccctat ttatagtcag gttagagttg ggaagatggg
    18481 taaattaatt aaaatataca aattacgttc gatgtgcaaa aacgcagaga aaaacggtgc
    18541 gcaatgggct gataaagatg atgatcgtat aacaaatgtc gggaagttta ttcgtaaaac
    18601 acgcattgat gaattaccac aactaattaa tgttgttaaa ggggaaatga gttttattgg
    18661 accacgcccg gaacgtccgg aatttgtaga attatttagt tcagaagtga taggtttcga
    18721 gcaaagatgt cttgttacac cagggttaac aggacttgcg caaattcaag gtggatatga
    18781 cttaacaccg caacaaaaac tgaaatatga catgaaatat atacataaag gtagtttaat
    18841 gatggaacta tatatatcaa ttagaacatt gatggttgtt attacagggg aaggctcaag
    18901 gtagtcttaa tttacttaat aagttcaaat aaaagttata ttttaaagat tgtgaccaat
    18961 tgttacagta taacgaggaa tcccttgaga cagtatcaaa tggcattaag aaatatgtgc
    19021 catcattgat ttgcatggct ataaatacta ttcatctgat gagatagcca tgttaagaaa
    19081 ttgaaagtat agcattaaag gggtttgtaa cagttgaaaa ttatatattg tattactaaa
    19141 gcagacaatg gtggtgcaca aacacatctc attcaactcg ccaaccattt ttgcgtacac
    19201 aatgatgttt atgtcattgt aggcaatcat ggaccaatga ttgaacaact agatgcaaga
    19261 gttaatgtaa ttattatcga acatttagta ggtccaattg actttaaaca agatatttta
    19321 gctgtcaaag tgttagcaca gttattctcg aaaattaaac ctgatgttat ccatttacat
    19381 tcttccaaag ctggaacggt cggacgaatt gcgaagttca tttcgaaatc gaaagacaca
    19441 cgtatagttt ttactgcaca tggatgggct tttacagagg gtgttaaacc agctaaaaaa
    19501 tttctatatt tagttatcga aaaattaatg tcacttatta cagatagcat tatttgtgtt
    19561 tcagatttcg ataaacagtt agcgttaaaa tatcgattta atcgattgaa attaaccaca
    19621 atacataatg gtattgcaga tgttcccgct gttaagcaaa cgctaaaaag ccaatcacat
    19681 aacaatattg gcgaagtagt tggaatgttg cctaataaac aagatttaca gattaatgcc
    19741 ccgacaaagc atcaatttgt tatgattgca agatttgctt atccaaaatt gccacaaaat
    19801 ctaatcgcgg caatagagat attgaaatta cataacagta atcatgcgca ttttacattt
    19861 ataggcgatg gacctacatt aaatgattgt cagcaacaag ttgtacaagc tgggttagaa
    19921 aatgaagtca catttttggg caatgtcatt aatgcgagtc atttattatc acaatacgat
    19981 acgtttattt taataagtaa gcatgaaggt ttgccaatta gcattataga agctatggct
    20041 acaggtttgc ctgttatagc cagtcatgtt ggcggtattt cagaattagt agctgataat
    20101 ggtatatgta tgatgaacaa ccaacccgaa actattgcta aagtcctgga aaaatattta
    20161 atagacagtg attacatcaa aatgagtaat caatctagaa aacgttattt agaatgtttt
    20221 actgaggaga aaatgattaa agaagtggaa gacgtttata atggaaaatc aacacaatag
    20281 taaattacta acattgttac ttatcggttt agcggttttt attcagcaat cttcggttat
    20341 tgccggtgtg aatgtttcta tagctgactt tatcacatta ctaatattag tttatttact
    20401 gtttttcgct aaccatttat taaaggcaaa tcatttttta cagtttttca ttattttgta
    20461 tacatatcgt atgattatta cgctttgttt gctatttttt gatgatttga tatttattac
    20521 ggttaaggaa gttcttgcat ctacagttaa atatgcattt gtagtcattt atttctattt
    20581 agggatgatc atctttaagt taggtaatag caaaaaagtg atcgttacct cttatattat
    20641 aagcagtgtg actataggtc tattttgtat tatagctggt ttgaacaagt cccctttact
    20701 aatgaaattg ttatattttg atgaaatacg ttcaaaagga ttaatgaatg accctaacta
    20761 tttcgcgatg acacagatta ttacattggt acttgcttac aagtatattc ataattacat
    20821 attcaaggtc cttgcatgtg gtattttgct atggtcttta actacaacgg ggtctaagac
    20881 tgcgtttatc atattaatcg tcttagccat ttatttcttt attaaaaagt tatttagtag
    20941 aaatgcggta agtgttgtga gtatgtcagt gattatgctg atattacttt gttttacctt
    21001 ttataatatc aactactatt tattccaatt aagcgacctt gatgccttac cgtcattaga
    21061 tcgaatggcg tctatttttg aagagggctt tgcatcatta aatgatagtg ggtctgagcg
    21121 aagtgttgta tggataaatg ccatttcagt aattaaatat acactaggtt ttggtgtcgg
    21181 attagtggat tatgtacata ttggctcgca aattaatggt attttacttg ttgcccataa
    21241 tacatatttg cagatctttg cggaatgggg cattttattc ggtgcattat ttatcatatt
    21301 tatgctttat ttactgtttg aattatttag atttaacatt tctgggaaaa atgtaacagc
    21361 aattgttgta atgttgacga tgctgattta ctttttaaca gtatcattta ataactcaag
    21421 atatgtcgct tttattttag gaattatcgt ctttattgtt caatatgaaa agatggaaag
    21481 ggatcgtaat gaagagtgat tcactaaaag aaaatattat ttatcaaggg ctataccaat
    21541 tgattagaac gatgacacca ctgattacaa tacccattat ttcacgtgca tttggtccca
    21601 gtggtgtggg tattgtttca ttttctttca atatcgtgca atactttttg atgattgcaa
    21661 gtgttggcgt tcagttatat tttaatagag ttatcgcgaa gtccgttaac gacaaacggc
    21721 aattgtcaca gcagttttgg gatatctttg tcagtaaatt atttttagcg ttaacagttt
    21781 ttgcgatgta tatggtcgta attactatat ttattgatga ttactatctt attttcctac
    21841 tacaaggaat ctatattata ggtgcagcac tcgatatttc atggttttat gctggaactg
    21901 aaaagtttaa aattcctagc ctcagtaata ttgttgcgtc tggtattgta ttaagtgtag
    21961 ttgttatttt tgtcaaagat caatcagatt tatcattgta tgtatttact attgctattg
    22021 tgacggtatt aaaccaatta cctttgttta tctatttaaa acgatacatt agctttgttt
    22081 cggttaattg gatacacgtc tggcaattgt ttcgttcgtc attagcatac ttattaccaa
    22141 atggacagct caacttatat actagtattt cttgcgttgt tcttggttta gtaggtacat
    22201 accaacaagt tggtatcttt tctaacgcat ttaatatttt aacggtcgca atcataatga
    22261 ttaatacatt tgatcttgta atgattccgc gtattaccaa aatgtctatc cagcaatcac
    22321 atagtttaac taaaacgtta gctaataata tgaatattca attgatatta acaataccta
    22381 tggtctttgg tttaattgca attatgccat cattttattt atggttcttt ggtgaggaat
    22441 tcgcatcaac tgtcccattg atgaccattt tagcgatact tgtattaatc attcctttaa
    22501 atatgttgat aagcaggcaa tatttattaa tagtgaataa aataagatta tataatgcgt
    22561 caattactat tggtgcagtg ataaacctag tattatgtat tattttgata tatttttatg
    22621 gaatttacgg tgctgctatt gcgcgtttaa ttacagagtt tttcttgctc atttggcgat
    22681 ttattgatat tactaaaatc aatgtgaagt tgaatattgt aagtacgatt caatgtgtca
    22741 ttgctgctgt tatgatgttt attgtgcttg gtgtggtcaa tcattatttg ccccctacaa
    22801 tgtacgctac gctgctatta attgcgattg gtatagtagt ttatctttta ttaatgatga
    22861 ctatgaaaaa tcaatacgta tggcaaatat tgaggcatct tcgacataaa acaatttaag
    22921 taccggtaat gctatacttt agaaaattaa gattaagaag aaaaggcaat ttcttattga
    22981 aaaatggaag ttgtcttttt taattctctt taaaagcggg aaacaaaagc agttaaatgc
    23041 ctttttgcat tcaatattaa atattatatc aatttcgaat atttaaattt tatataattg
    23101 gatataacaa ataaataata attattgcaa aacacacccg aaattaatta ttataaaagt
    23161 atattcataa aaggaggaat atacttatgg catttaaatt accaaattta ccatatgcat
    23221 atgatgcatt ggaaccatat atagatcaaa gaacaatgga gtttcatcac gacaaacatc
    23281 acaatacgta cgtgacgaaa ttaaacgcaa cagttgaagg aacagagtta gagcatcaat
    23341 cactagcgga tatgattgct aacttagaca aggtaccgga agcgatgagg atgtcagtcc
    23401 gtaataatgg cggtggtcat tttaaccatt cattattctg ggaaatacta tcacctaatt
    23461 ctgaagaaaa aggtggcgta atagatgaca tcaaagcgca gtggggcact ttagatgaat
    23521 ttaaaaatga atttgcaaat aaagcaacaa cattatttgg atcaggttgg acttggttag
    23581 ttgttaatga tggcaaatta gaaattgtga caacgccaaa ccaagataat ccattaacag
    23641 aaggcaaaac accaatctta ctatttgatg tttgggagca tgcctactat ctgaaatatc
    23701 aaaataaacg tccagactat atgactgcat tttggaacat tgttaactgg aaaaaagttg
    23761 atgaattata ccaagcagca aaataatata acttaatata attgaggtgg agcatctaca
    23821 aggtgttcca cttttttgta cttaatatct ttacttattt gtttttgtgt aatgggatag
    23881 cacgtaaaag tggaaaaata ataagataaa tttcaactcg aaatacctta ttattgttga
    23941 ctataacgat gacctaaata ttatatacat tagacaacac tattaattaa tcaacatatt
    24001 gacattaaat aaattgacaa aataagtaat tattgtgaat ctaaagtgaa atttttataa
    24061 aaaaatgtaa tgattcaaaa attttgttgc atttcttttg taatcgtatg ataatgtaaa
    24121 tgtaatcaaa ttgtaatata aggggacaag acaatgaaaa aattagcaac agtaggttct
    24181 ttaattgtaa caagcacttt agtattctca agtatgcctt ttcaaaatgc gcatgccgac
    24241 acaacttcaa tgaatgtgtc gaataaacaa agccaaaatg tacaaaatca tcgtccttat
    24301 ggcggagtag taccacaagg aatgacgcaa gcacaatata ctgaattaga gaaagcttta
    24361 ccccaattaa gcgctggcag taatatgcaa gactataata tgaaattgta tgatgcgacg
    24421 caaaatattg ctgataaata caatgtgata attacaacta atgtaggggt atttaaacca
    24481 catgctgtta gagatatgaa tggccatgcg ttacctttaa caaaagatgg caatttttat
    24541 caaacgaatg tagatgcaaa tggtgttaat catggtggta gtgaaatggt gcaaaataaa
    24601 acaggtcata tgagtcaaca aggccatatg aatcagaaca cacacatgaa ccaacagcca
    24661 cacatgcaac aaggtcatat gcaatcatca aaccatcaaa tgatgagtcc aaaagcaaat
    24721 atgcattcat caaatcatca aatgaaccaa agtaacaaaa aagttttacc agctgctggt
    24781 gaaagtatga catcaagtat tcttactgca agtattgccg cactactatt agtatctggg
    24841 ttattcttag catttagacg acgttcaaca aataaataaa cataatacga ttaataatag
    24901 aaaaatcgtg tgattatctg agcgagccta ggacataaat caatgtccta ggctcgctaa
    24961 tgttatattg gcagtagttg actgaatgaa attgcgcttg taacaagctt ttccatttct
    25021 taccaactcc ctaaatagtc atcaaaaaat ttcttatatt ttaataattt ttaataatcc
    25081 gattgtctta tacgtgtcag tgttaattca gatatttcct gtggaatata ccacttatta
    25141 atcataattg gataaggtgt ttgtgcgtac agtgtttcaa taatcagcca acaatgtgta
    25201 tcaccatcaa acacgtgact atgatttttg aagtggggcg ctttggtaat agacattttt
    25261 aaatctgatt gatatgcatt gctataaatc gtttgctcaa cgaatgtctt catgtcgtct
    25321 tcgttttgtg tattcacttt aaatgtgtca atgacattta acggtataaa ggtaaagcaa
    25381 aatgcatcag cttgcttaga atgattgtcc tttttttgat aatagcgttc cattgcaatg
    25441 acggcagaag gatggtttgc aaacaaatga tttgtatatt cactttctaa atcaacacga
    25501 taattaattg atgacataga tacgcgagct agcaatattt gatcaagtgg atgcttaaat
    25561 tgatccatac ttgaagcgtg ttgggcattt gtttgtggaa taacaaagtg tcccttccct
    25621 cttgtactct ctacgatgcc atcttcggct aacaatttta tagcttggcg caaagtcata
    25681 cgactgacat caaagcgcgc acaaagttcc ttttcagtag gtaatgcatg gccactcgga
    25741 tattttccta tttgaatttc tttatataac gtattataaa tcgttaaaaa ttttggttgt
    25801 gtttgcgtca cgtagacaac ctccataaag ttacttaatc actctcatca tacaataatt
    25861 tttactcaaa ttggaaaaat tataaaaatt aaatatagat aggctttgaa aattagtttt
    25921 atacaaggtt agtagctgta actgtaaaat gttcttaata ttgtcaaaat gtaatgcttg
    25981 aaagcgcttt taaaaaatat tattatatac atggttagac aaatagacaa atcactatac
    26041 aaatattggg aggaatattt tatgaaatca acaccacaca ttaaaccaat gaatgacgtc
    26101 gaaattgcag aaacggttct attgccagga gatccgttaa gagctaagtt cattgcagaa
    26161 acttatttgg atgatgtgga acagttcaat acagtgcgaa acatgtttgg ttttaccgga
    26221 acatataaag gtaaaaaagt ttctgtcatg ggttcaggta tgggtatgcc atctattggc
    26281 atttactctt atgaattaat tcatacattt ggttgtaaaa aattaattcg cgttggctct
    26341 tgtggcgcga tgcaagaaaa cattgattta tatgatgtga ttattgcaca aggtgcctct
    26401 actgattcaa attacgttca acaatatcaa ttaccaggtc attttgcgcc aattgcttct
    26461 tatcaattat tagaaaaagc agttgaaaca gcacgtgaca aaggtgtacg tcatcatgta
    26521 ggtaatgtgt tatcaagtga tattttctat aacgcggata caacagcgag tgaacgttgg
    26581 atgcgtatgg gtattttagg tgtagaaatg gaatcagctg cattatacat gaatgcaatt
    26641 tacgctggtg tcgaagcatt aggtgtgttc acagtgagcg atcatttaat tcatgaaacg
    26701 tcaacaacac ctgaggaaag ggaacgtgca tttacagata tgattgaaat tgcactgtca
    26761 ttggtgtaga tgattatgaa tgttgaatat tctaaaataa agaaagcagt acctatttta
    26821 ttattcttat ttgtattcag tttggttata gacaactcat ttaaattgat ttctgtagcc
    26881 attgctgatg acttaaacat atctgtaacg acagtaagtt ggcaagcgac attagccggt
    26941 ttagtaattg gtattggcgc tgtagtatac gcttcattat ctgatgccat tagtatacgc
    27001 acactattta tttatggcgt gatattaatc attatcggat caattattgg ttacattttc
    27061 caacatcaat tcccattact tttagttgga cgtattattc aaactgccgg tttagctgct
    27121 gcagagacat tatatgtgat atatgttgca aagtatcttt ctaaagagga ccagaagact
    27181 taccttggct taagtacgag cagttattcc ttgtcattag ttatcggtac attatcaggt
    27241 ggatttattt ctacgtattt acactggaca aatatgtttt taattgcatt aatcgtagta
    27301 tttacgttgc cattcctatt taaattatta ccaaaagaaa ataatacgaa taaagctcat
    27361 ttagattttg ttggcttaat tctagtggca actattgcta caacagtcat gctgtttatt
    27421 acgaacttta attggttata tatgattggt gccttaattg cgattatcgt ttttgcgcta
    27481 tatattaaaa atgcgcaacg tccattagta aataaatcat ttttccaaaa taaacgttat
    27541 gcttcatttt tatttatagt atttgtaatg tatgctatcc aattgggtta tatttttacg
    27601 ttcccattca taatggagca aatttatcat ctgcaactag acacaacatc actgttatta
    27661 gtaccgggtt atatagtagc agtcattgtt ggtgcactaa gtggtaaaat cggcgaatat
    27721 ctgaattcaa aacaagcgat tatcacagca attattttaa tagcactgag cttgatttta
    27781 cctgcatttg cagtaggtaa tcacatttca atcttcgtca tttctatgat attctttgca
    27841 ggtagctttg ctttaatgta tgcaccttta cttaacgaag ccattaaaac aatagatctt
    27901 aatatgacag gtgtggctat tggtttttat aatttaatta ttaatgtggc ggtatctgta
    27961 ggtattgcga ttgctgcggc tctaatcgat tttaaagcat taaatttccc aggcaatgat
    28021 gcattaagtt cacatttcgg tattatttta attattttag gtttaatgag tattgtcgga
    28081 ttagttttat tcgtcagctt aaatcgttgg acacaatctg aaaaataaat agatataaat
    28141 tcgcgagata tattcgtatt tatagtaaaa ttaaataaag agattatata acacgaggag
    28201 tagtaagtat gaaatttgag aaatatatag atcacacttt attgaagcct gagtcaacac
    28261 gtacgcaaat cgatcaaatc atcgatgaag cgaaagcata caattttaaa tctgtatgtg
    28321 tgaatccaac acatgttaaa tatgcagcag agcgactagc tgattcagag gtgctcgttt
    28381 gtacggtaat aggattccca ttaggtgcgt cgacaactgc aacgaaagca tttgaaacag
    28441 aagatgcaat tcaaaatggt gcagatgaaa ttgacatggt catcaacatc ggcgcattaa
    28501 aagatggacg ttttgatgat gtacaacaag acattgaagc agtggt
//
LOCUS       DALAAR010000034        26357 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000034 DALAAR010000000
VERSION     DALAAR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.035"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            247..1599
                     /locus_tag="O2458_001462"
     CDS             247..1599
                     /locus_tag="O2458_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCZ3887329.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(1661..2848)
                     /locus_tag="O2458_001463"
     CDS             complement(1661..2848)
                     /locus_tag="O2458_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887330.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(3244..4020)
                     /gene="fetB"
                     /locus_tag="O2458_001464"
     CDS             complement(3244..4020)
                     /gene="fetB"
                     /locus_tag="O2458_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCZ3887331.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(4013..4675)
                     /locus_tag="O2458_001465"
     CDS             complement(4013..4675)
                     /locus_tag="O2458_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3887332.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(4923..5999)
                     /locus_tag="O2458_001466"
     CDS             complement(4923..5999)
                     /locus_tag="O2458_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ3887333.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(6354..6620)
                     /locus_tag="O2458_001467"
     CDS             complement(6354..6620)
                     /locus_tag="O2458_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCZ3887334.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(6620..6877)
                     /locus_tag="O2458_001468"
     CDS             complement(6620..6877)
                     /locus_tag="O2458_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ3887335.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            complement(6983..7177)
                     /locus_tag="O2458_001469"
     CDS             complement(6983..7177)
                     /locus_tag="O2458_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887336.1"
                     /translation="MMLFKNLNFVIFFEDMSFFKRIPHKILKIFKPENDKAVGNVVCS
                     TADFFLSIIGGMLEEKNKSL"
     gene            7236..7691
                     /locus_tag="O2458_001470"
     CDS             7236..7691
                     /locus_tag="O2458_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCZ3887337.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(7859..9436)
                     /locus_tag="O2458_001471"
     CDS             complement(7859..9436)
                     /locus_tag="O2458_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCZ3887338.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            9631..10380
                     /locus_tag="O2458_001472"
     CDS             9631..10380
                     /locus_tag="O2458_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887339.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(10604..11797)
                     /locus_tag="O2458_001473"
     CDS             complement(10604..11797)
                     /locus_tag="O2458_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887340.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(11809..12558)
                     /locus_tag="O2458_001474"
     CDS             complement(11809..12558)
                     /locus_tag="O2458_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887341.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(12551..13366)
                     /locus_tag="O2458_001475"
     CDS             complement(12551..13366)
                     /locus_tag="O2458_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887342.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(13363..14232)
                     /locus_tag="O2458_001476"
     CDS             complement(13363..14232)
                     /locus_tag="O2458_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887343.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(14229..15164)
                     /locus_tag="O2458_001477"
     CDS             complement(14229..15164)
                     /locus_tag="O2458_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887344.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(15177..16775)
                     /gene="cntA"
                     /locus_tag="O2458_001478"
     CDS             complement(15177..16775)
                     /gene="cntA"
                     /locus_tag="O2458_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCZ3887345.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFILISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(16918..18219)
                     /gene="cntM"
                     /locus_tag="O2458_001479"
     CDS             complement(16918..18219)
                     /gene="cntM"
                     /locus_tag="O2458_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCZ3887346.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(18212..19030)
                     /gene="cntL"
                     /locus_tag="O2458_001480"
     CDS             complement(18212..19030)
                     /gene="cntL"
                     /locus_tag="O2458_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCZ3887347.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(19041..19862)
                     /gene="cntK"
                     /locus_tag="O2458_001481"
     CDS             complement(19041..19862)
                     /gene="cntK"
                     /locus_tag="O2458_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCZ3887348.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(20729..21094)
                     /locus_tag="O2458_001482"
     CDS             complement(20729..21094)
                     /locus_tag="O2458_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887349.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            complement(21357..22175)
                     /locus_tag="O2458_001483"
                     /pseudo
     CDS             complement(21357..22175)
                     /locus_tag="O2458_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            22408..23946
                     /locus_tag="O2458_001484"
     CDS             22408..23946
                     /locus_tag="O2458_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCZ3887350.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(24058..24480)
                     /locus_tag="O2458_001485"
     CDS             complement(24058..24480)
                     /locus_tag="O2458_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCZ3887351.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(24932..25351)
                     /locus_tag="O2458_001486"
     CDS             complement(24932..25351)
                     /locus_tag="O2458_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ3887352.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            complement(25369..25788)
                     /locus_tag="O2458_001487"
     CDS             complement(25369..25788)
                     /locus_tag="O2458_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ3887353.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINESKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            complement(26055..>26357)
                     /locus_tag="O2458_001488"
     CDS             complement(26055..>26357)
                     /locus_tag="O2458_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ3887354.1"
                     /translation="KEQQERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYIN
                     ENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
BASE COUNT         8395 a         5035 c         3857 g         9070 t
ORIGIN      
        1 tatttttaag tacacattag ctgtggctaa tgtataagaa ccactacata ataaatcatt
       61 tgtggctctt tatcatttct gtcccactcc cgtagaagta catcatataa tgctgaaaat
      121 ggtttgagtt aaaacagata tcaagctcgt ctgattcagt cacaaaattg tcttgttata
      181 cttgtcacct atcatctata gaccgtggta tgattaaatt ggggatgata aaggaggtta
      241 ataaatatga agattaatac tacaggtggt caaattcatg gtattacaca agatggttta
      301 gatatcttct taggcattcc ttatgcagaa ccaccagttc atgacaatcg ctttaaacat
      361 tctacgttaa aaacacaatg gtcagagcca attgatgcaa ctgaaataca acccatccca
      421 ccgcaaccag acaacaaatt agaagatttt ttctcctcac aatctacaac ttttactgaa
      481 catgaagact gtttatatct aaatatttgg aaacaacata atgatcagac gaagaaacct
      541 gtcatcattt atttttatgg tggtagtttt gaaaatggtc atggtacagc cgaactctat
      601 caaccggcac atttagtaca aaataacgac attatcgtta ttacatgcaa ttatcgttta
      661 ggcgcattag gatatttaga ctggtcatat tttaataaag attttcattc caataatggc
      721 ctttcagatc aaatcaatgt cataaaatgg gtgcatcaat ttattgaatc cttcggtggc
      781 gacgctaata acattacttt aatgggtcag tctgcaggca gtatgagcat tttgacttta
      841 cttaaaatac ctgacattga gccatacttc cataaagtcg ttctactaag tggcgcacta
      901 cgattagaca cccttgagag tgcacgcaat aaagcacaac atttccaaaa aatgatgctc
      961 gattatttag atacagatga tgttacatca ttatcgacaa atgatattct tatgctgatg
     1021 gcgaagctaa aacaatctcg aggaccttct aaagggcttg atttaatata tgcgcctatt
     1081 aaaacagatt atatacaaaa taattatcca acaacgaaac caatttttgc atgttataca
     1141 aaagatgaag gcgatattta tattactagt gaacagaaaa aattatcgcc gcaacgcttt
     1201 atcgacatta tggaattaaa tgatattcct ttaaaatacg aagatgttca gacggcgaag
     1261 caacaatctt tagcgattac acattgttat ttcaaacagc cgatgaagca atttttacaa
     1321 caactcaata tacaagattc aaacgcacaa ctatggcttg ctgaatttgc atggcatgat
     1381 acttctagcg cacactatcg cagtgcttac catattctag atatggtatt ttggttcgga
     1441 aacttacaaa tattagcagc acatcaatat ccaacaactg ctcaccttaa atttttaagt
     1501 cgacaaatgc aaaatgattt agctaatttt gctaaaagcg gtaaaatgcc ttggccgatg
     1561 tatcataatg aacgtcgcta ttatcgcaca tatcaataac gttatcgcat ctaaaaaagc
     1621 acacctgaat ttgatagcag gcgtgctttt catatgagaa ttatgatgat ttaaatgttt
     1681 tagcataatg attatttttc aatgtgaaaa caacgattaa tcctaatgcg ccaatgatgg
     1741 cactcgtata tgtgatagct tgaacagaaa catgtgtcat gaccaagcct ccaataatgc
     1801 ctccaacacc aataccagcg tttaaactag acatgttcca actcattact tggcttgtgt
     1861 cgccttcaac atgttgaatc acaccgcttt gcactgctgg attagtactc cattgcatga
     1921 tattccaaat aaatagtcct gctaacaata gacctgaacc aggtaagatt aaattcataa
     1981 gtaacatcat gacgataaaa atagaaacag aaatcattaa ccaacgctta cttgtaattt
     2041 tatcggagaa tataccacct aatgatgttc caataacgcc agcgattcca tttactagaa
     2101 gtgctaatga aacgaatgac atatcatgac cattagataa aataagtgga tttataaaga
     2161 cgaatgtcac tgagtttgca atcaatacta aaaacgtaat aattaaatat tttgctactt
     2221 cagcaggtct taatattttc gaagtaacat gattttcatg agatggtgtc tcatgattca
     2281 cagggcctcg ttgtatttcc tgatccttcg gtaaatagat catcatcaag aagccaacaa
     2341 taatactcac aataattaag aatagaaatg tatagcgcca acctactaaa tcgccgataa
     2401 ccgttccaat tggtacacca aaaacattag caccactaaa ccctgtatag acaagtccaa
     2461 tcattttacc acgattttta ggtgctgata acatcgctgt aatagctaat acttttacga
     2521 taattagtgc tgctgctgca gatgagataa ttctaccaac tactaatatt gaaaaatttg
     2581 gcgctacagc aataatgcca ttaccaatga taaatataag taatgtccat aataatacag
     2641 gccttgatga aaaacggttc gttaatttaa ccagaatagg tccacatatc gcaaatgtta
     2701 aagcgtacat tgtcactaat tgaccaacga cagcttctga tacatgtaag tcctgactca
     2761 tcaagttcat aattcctgca accatcattt caaccattcc tacgataaaa atacttaaaa
     2821 tgaatgtaaa tagtcgcatg attgacaatt taaaactcct ttcaaacaaa atttccgata
     2881 ttcatttccc ttagaataac gatttattaa aaaatgtgtg caacacaatt ttatatataa
     2941 ccgttctatt atataatatt ttgctttaat tttctgtgct taattggaaa attattgaaa
     3001 ttgtttttca gtatttgaaa aattaacaca agtatgtcat ttaataatta tgtataatta
     3061 cttcagtttc aacaataact ccaaaaaagc acgcacgctt tatatgtcag aacagataac
     3121 tgactacaaa gtatgcgtgc ctttatgaat attttcgctg cgctaatgaa tagcgatact
     3181 atatgtgcgc gtcacttcat ttccaattaa aactaatgcc taaatctgat gcagtaaaat
     3241 ctatcatgat tcactcttaa catcagtatt tcgtgctact aattgatgtc ttgcattgaa
     3301 aaattgacca tagcttaaat atgtcgcaat caaagcagac ataatggtcg cagttgtatg
     3361 aataaacacg actaacaatt gaaatttaat cgcttgtaaa ggtggtacgc caccaataat
     3421 taagcctgtc atcataccag gaatcgacac aagcccatat gttttaaccg aatcaatagt
     3481 tggcactata gctaaacgaa tactttcacg tattgcacct ttagaagcca atttaggtgt
     3541 agctgcaagt gataatttag attcaatatt agtaccatct tgtacgaatg cacgatctaa
     3601 attctggtaa gctaaattaa ttgcaatcaa gccattattt gcaagcatgc cgccgatagg
     3661 tataacttca tttgcggtaa attgaatggc ccctgtcgca atagtacctg caagcggtaa
     3721 tgccgttcca atgaagatag ctagaaatga tatccaaaac acatgatgca tcactggtga
     3781 tgctcgacta attgtattcc atgatgcatt aataataatg accaatacac ataaaataag
     3841 cagccattta tcgtttattt taaaaatata atgcagcaaa aatcccaaaa tgattaattg
     3901 cacaactgct cgtaatgtcg caacaattaa atctttaata atatgtaaac cttctttata
     3961 tgaaatgata atcggaatga ctaaaagcaa tgccgtcaat ccaagtgccg tattactcat
     4021 gttgattcaa ctcctctgta ttagaaattt gaccatcaac aattgttata cgcttttgaa
     4081 agtgtcgcat actttggtca tcgctgtggg taatccacat aattgccacg ccttgatctg
     4141 ctaatttaaa aatgatattt tctatctttt ctttattatt aacgtctaat gcactggtcg
     4201 attcatctaa taaaagaata tccggtgtat acatcagttg gcgcgctata gcaattcttt
     4261 gccgctcacc acccgacata ttttccactt ccgaacttaa ttgataatgt cccaaaccga
     4321 catctttaat taattgcttt gcacgttttc tatcaaattt atcattacgt gcaagtgatg
     4381 ggaatatcat gttatcttca atcgtttcac caaacaagtc actttgctgc atcaaataac
     4441 tgattcgttg acgcaattct tccgggtcat aatcattata gggtttacct ttaaaataaa
     4501 gttctccact agttggacta aacaaattac atatttgctt ttgaaatgta cttttaccac
     4561 tacctgatgg acctataatg gcaatactct cgcctttatc tacttttaga ctgatatgat
     4621 ctagtatgat tctatcgctc gctttataca ctaaatcttt aatttctaac aacatttgca
     4681 ccgctcctca tgtaatatat caggtatatt attcccgttt ttttggtttt tagtgcaatt
     4741 acatttaaga aaaaatcatt ttcttgtaat atcaaaattt aggcataatt gattttcagc
     4801 atttaaatta aaaacaaaaa atcctattaa catggatact gtctaatgac taagaattaa
     4861 aattagacaa ttgtttaaca acctaccata ttaaaaggat tctttattat ttatggattt
     4921 gattaccaca tgatattttt ataactttca tcattcaatg aacggacaat ttcagtaaca
     4981 agacggatag aattttcata atcatctaca ttgagcactg aaacattaga atgcatgtat
     5041 cgcagcgtaa caccgattgt catcgttgga ataccttcat ttgcgacatg aatacttccc
     5101 gcatctgtac ctccacctgg tgtcgtatcc cattgtactt cgatgttatg ttccttagct
     5161 acatctttaa tatgctttcg caaaccttgg tgagcaatac ttgtagcatc catcataatg
     5221 acaactggac caccgcctag tttactatcg ctcgtttgac ctgacatacc tggggtatca
     5281 taagcaatac ctacatcgac agctatcgcc aagtctggtt taatcgtatt cgctgccact
     5341 ttcgcaccac gcaaaccaac ttcttcttgc actgtggcac cactgtataa gttaatgcca
     5401 atattttcat cttttaaacg ttttaatacc tcaatagcta atgcacagcc atagcgatta
     5461 tcaaatgctt tcgcagttaa atatttatca tttgcaagca cttcaaattc actatatggc
     5521 gtaaccatat tgcctacttc aacgccagct tcttcagctt cttccttgct actaacacca
     5581 atatctataa acatattttt gatttccatt ggctttttac gttcttcagg cgttaagaca
     5641 tgtggcggtt tagaaccgat gatacctcta atttctttgc ccgaatctgt tgtaatcgtt
     5701 actttttgag atagcatgac ttgattccac catccaccaa ctggcgtaaa tgaaataaaa
     5761 ccatgtttat caatctttgt taccataaaa ccaacttcat ccatatgacc agaaatcata
     5821 attgagtatt gaccattctc agcatttttc tttccaaaaa tgccacccaa gttatcttca
     5881 ataatttgat cactgacagg ctctatatag ttacgcattg cttctttaac ttgcatttca
     5941 taaccagcaa tgccgtttac atctgttaaa tgtttaagta atttgactga ctgattcatg
     6001 ttattcatcc tcctgtattt caacttaaaa ctattgtatc actaactttc agaaaaacgt
     6061 tccctaccgc tttatcattt tcaggcttaa atatttttaa aattttgtga ggaatccgct
     6121 taaaaacctc atatttctaa aatttaatat tccgaaaaag tcacaattta aaattcctta
     6181 ttataatacg ttattctttt attgttacta atttcaatct gtttagtctt gcgctattat
     6241 aaaattaaaa agtgtagaga taacaacgca ttaacgtcgc tattttccct acacttaaat
     6301 actagtttct ttaattaact tatcattaaa gttattcaga tattgaatat tcattaatca
     6361 taatgtgacc atgccgatag tattaatact tctttatttc gatcatctac ggtatagacg
     6421 accctgtgct gatggttaat tcttcttgaa tatcgctcta aatatttagg ctctaatttt
     6481 tcaaaagatt gtgttatttt atacggatca ttttttaaag tctcaacaat ttctaaaaat
     6541 gacttcttta aataagaatg ttttattttc tttaaatctg atttcgctga atttttaatc
     6601 ttaaccgtgt aattgctcat taaagattat cccaatcaat atcatctata tttgttgtac
     6661 cactattatc tttttctctt tctcttactt tgtccattgt accagtagat tcaagatata
     6721 ttgtctcttg tatacttttc caatcttcta aacctataat cacagcatta ttttcggcat
     6781 tattgccact aatataaatt ggttcgtgat tattattaac attttttagt aattgataaa
     6841 aatcttttct cgcttctgta gggctagtga taatcatata aaccctcctt tgttttctgt
     6901 tacgctaatt atagcgtacg cacttgagta cgtcaatatt accaacgagt tcatcgtatt
     6961 cttaatacct atatgctatc gttcacaaac ttttgttctt ttcttccaac ataccgccta
     7021 taatgctcaa aaagaaatca gcagtggaac acactacgtt ccctaccgct ttatcatttt
     7081 caggcttaaa tatttttaaa attttgtgag gaatccgctt aaaaaaactc atatcttcaa
     7141 aaaaaatgac aaaatttaaa tttttgaata acatcatttt ttagtaaatg ttataatttc
     7201 tttataatac aaatatcgaa agaaggcgac tgactttgaa aaaattatgt tcattaattg
     7261 tagtagcatt agtttgtatc attgcattat cagcttgtgg taaagaacaa accaaaacat
     7321 atgaaggtga tgttagtggt aaacatgtac taacttctat cacatataaa gatgataaag
     7381 ttttaaaaca gtctacaatc aacacaatta aatatgacga tctaggtatg gataaagacg
     7441 aagccaaaaa gttattcgcc aaatctgaaa gtattttcaa agaccttaaa ggcgtaaaat
     7501 acaaagtaga ctataaagat aaaaaagcaa ttgaacactt agacatagat tacacagaag
     7561 ttgacatgaa aaaattaaat aaacgtcttg gtgtttcgac taaagaaaat aaagatatta
     7621 gttttgaaaa acttgaaaag caattaaagc acagaggttt aaaagaaaaa gataaaatgg
     7681 acgacaaata gtttataact taaaaatgcc ctcagataag actaaggtta caaaccttaa
     7741 ttcatattct gagggcttta atatttgaag ttcttgtgtg accagcatcc actactaata
     7801 taaaattatt tgcagtaacg ctaaaatccg ctgctttcaa tttcccgaaa taattaagtt
     7861 aactaatgag ttttaattta taatcatgta tcgtttgtaa ctcaccatcg acttttcgat
     7921 atacaatatg atcagcagta atttctgtag gactggatac gccaacagct gctgcaatat
     7981 tgaataagcc ttcatgcaaa cttgttacat agtttgtgac acgatattgc ttttctccaa
     8041 caatcaatgc tttttctttc ttcgcatctg tcgttgcaac acctacagga cacgtattca
     8101 tgtgacattg ttgactcatt atacaaccga cactaatcat catcccacgt gcgatattta
     8161 caaaatctgc acctaaacct agtgcaatcg caattttatc tggtgtcact aacttaccag
     8221 atgccgccaa tttcacttta tctcgaatac catatttttc taacatgcca gacacaatag
     8281 gtagagctgt aaatagcggt aagccaacac catcttgtaa ttcttggaat gttgcaccag
     8341 taccaccttc accaccatca atcgtaataa agcttggata cttatctagt tccaccatcg
     8401 tacgtacaag tgtttcaatt tctgaaactt tgcttactac aattttgaat cctactggtt
     8461 tttgacctaa ttgctgcaac tgatcgacga aacgaatcaa atcttcagca ttatgaataa
     8521 attcgtaacg gttaggtgaa ttgattgttt tataaggttc aacatttcgg attttagcaa
     8581 tttcttcgtt taccttttca gcttccatat gaccaccacg agtcttagca ccttgtgcca
     8641 acttcagctc aaatgcgcgt acgttagata actgtgcaac ctctttaaat aaaccttcac
     8701 taaaattacc ttctttatca cgaacaccaa ataaaccggg accaatttgg aaaatgatat
     8761 ccccattacc ttttaaatga tattctgata agccaccttc acctgtattc atccaagtgc
     8821 ccgctttagc tagaccttta gataaagctg taatggcatt ttttcctaaa gcgccataac
     8881 tcataccaga ttgtcctacg atacgtttta aaataaatgg atgttttaaa tgttcaccta
     8941 attttattgc atggtcatca cttaagtaat acggatcaat ctttgtcggc acacgatatt
     9001 cttcacgact aaataaacgc tcattcgcga ttttataaat gaatgttgat aacaatgttg
     9061 tattatctac tgaaatctca ttacgttgca tcggaaacat tgtgttctgt atgtaaaagc
     9121 cgtcttgata atctttagta gtaccgaagc tggtcatacg agagttatat tttccagcca
     9181 aaacgatatt tttataatca ttacgtgaaa aaggtttccc ttcattatcc ccagaaaata
     9241 aatactgacg taattccggt cccatttttt ctgaaatata tctaatacgt gctagtaaag
     9301 gataattcct taatacacta tgttgtgatt gtcttttatc tttaattaac caaataagcc
     9361 cgataacaat aaccgtaagc atgaatccta caacgataat gttaactata aattgcatga
     9421 ctgtaagaaa cgtcattaca atacctcccc ccaaaatttc aattcaatat ttatgataca
     9481 ccttacaaaa caaaacacaa tggaagcgct tcattttata aaacaatttt atgatatgtt
     9541 tttcatttta aattttaatg tataaaacat acaatacaaa gtaatatgtg ctaaagtatc
     9601 tatataatac aactatttaa gaggtatact atgtcaaata caaataaaca ttacatagaa
     9661 gaagaatacg ctaccgaaca atcgcgtttt ttcaaacgtg atattggatt tattttcctt
     9721 tacatatttt tggttaacat cttgccgatc attatcgtct ttttaacttt tgggttagta
     9781 gctgtattca ctcaacaagc tccgtggggt ttctcttttc ctatttcaat gaacattagc
     9841 tatttactcg cagttattat ttcactagct atttttcgtc tgatgcatga agatgcgttt
     9901 attccaatga tacgttcgca aatcgttgct agtgctaaat ataaatggca tattcttata
     9961 tcatttacac taagtgtcgt actcttttta cttttaaatc atttcaatat tttcgcacta
    10021 aatgtagatt cctttactgt ctttgacatt tacccgctca ttaaagggta tggttccatc
    10081 agtttaataa ctatattttc gttaaccatt atattaaata ctttgatttg tcaacttatt
    10141 ttccgtcata ttttaattca agaattaagc agattaatac ctttaaagat tacaatagta
    10201 ttgtccatta tccttgaaac tctgttctac tatccataca ttaatacatg gtgggaattc
    10261 attccagcat tgatactagc aggtagtgcg acttacttat atttaaaatc ataccgtaat
    10321 tttatggtga gctatttcta tcaattagca gttatactcg ttttgcatat tttcatgtaa
    10381 aatttttcag agtattatcc attatccata cacatcaaaa agcacactta aaagtcagta
    10441 tcaaaactca cttttaagcg tgctttattt agatatactg tcgttctaaa aacgagccat
    10501 atagaatgtt tcatgtccta gcatgaagca catgaatcgc catgctcaat atacctttta
    10561 aaatttaaat attcattaac ttaatatgct ttaattcact tcattaaaga ctactcgctg
    10621 gacgtggtgt tggctttttc aagaaactca taatcattat tacaatgact aacataccac
    10681 aaattagata catccattta aatcctacta aatcggaaat aggtcccatc aatgcgccac
    10741 ctaacgatat acctaagtct gcacaggcaa taaataaacc taacaacata ttacgaccta
    10801 cttttggtaa gacgaagctt aagtatgatg ttaatgttgg gtacaccatt gcttgcgtca
    10861 ttcctattaa tatcgcacta ccatagaaaa taattgcacc tacttgcgga ccaaatgcca
    10921 ctacaaatga cgcgattact aataatgata gtacagatac catatattta ggatgccaca
    10981 taccatctga cggaatgtat ttccttaagt aaaatcttgc cgcaacaact gcgatggcct
    11041 gtattgtcaa aaagattccc gcattcgcga atcctaaact cactgtgtat aacggtacaa
    11101 atgtactaac tgcaccaaat acaatcgatg caacaatcat gataatacca ctgttcaaca
    11161 actctttatt tttgaaaaat tgcgcaaaaa cagttacagc gttaaacggc attttttcaa
    11221 tcttatctga cgtatcgggt tcctgttcag caaaggtcac gcgatagcca aagaatgttg
    11281 ttgttaatgc gataaagata atgacaattg caaatagtga aatattattt gcattccaaa
    11341 tacctacggc aactaatggt ccgattaagt ttggaatcgt tgaaaatagc gagtacaatg
    11401 atacaccttc agaacgatgt tcctctggta atgcatcaat aatacctagc tgtaaagaca
    11461 tagaaaagaa tgccgtacac acaccttgca taacacgtgc tacgaaataa ccttctaagc
    11521 cagtaaaacc ataaataatt aaagctatgg cattgataat caaaataatt cttaatactt
    11581 taatgggacc gacacgagca ataatttgtc ctgcccatgg tcgaaatacc attgctgtta
    11641 acatgtatgc ccccataacg ataccgataa ccgtattcgt tgcgcctaaa tcatgccctc
    11701 gtaaaggtat aaacacgttt aagattgcat tggcactaaa gaacatcaat gttaatatat
    11761 ataatctcaa aaagggccaa gccattgcac ctttcattat ctcactcctt agaatgatag
    11821 ttgtttttct attaattctc ttgtataagc attgtcactt ttatgcaatg cgcttgtcgg
    11881 aatttgttct tctatttttc cgtttttaaa aataattaat tgatcacata aatacgtggc
    11941 agcttgaata tcatgtgtga taaaaatata actcaactga cgcgtttcac gtaaatgaat
    12001 caataaatct aatatttgtg tttgaattga catgtcgagt gaactaatgg cttcatcaaa
    12061 caaaatatat ttagggttaa tacatattgc acgcgcaatc gcaacacgtt gcgcctctcc
    12121 acctgataac atattaggat atttatccat gtatgcctta gatagaccga cttcttccaa
    12181 caatgtaatt gcttggactt ccataacttc tttaggttgt ccatcacatt gacacatcac
    12241 ttcaaataag atttctctaa cagtctgaaa tggatgtaat gatgacgtat aatcttgaaa
    12301 tacagcacca atttgatgac gtctcacttt cttcttatgc atcggttgat catttaaggt
    12361 tacacaacct ttatccggtt tctcaatacc taatatcata cgactcaacg tcgatttacc
    12421 gctaccactt tctccgataa tcgcaatcgt cgcaccgatt ggacactcaa atgacacacc
    12481 tttcacgata ggtgttcgac gacgcttaaa aacatgtgcg ctttgatatg acttttcaac
    12541 atctttaatt ttaatcatgt acatcacccc tcatcacatg tttaaaatga tcattaatct
    12601 tcttcttcgt tgataataaa tacttcgtat aaacatgttc tggatgatgc aagactgatt
    12661 cacgtgtccc ttgttcaata agctgaccat ttttcatcac aacaacacgg tctgcaatct
    12721 tgttaataac cgttaaatca tgtgaaatga aaatcatcgc acagtcaaag tgttttttaa
    12781 tatctataaa tgcttccagt acatcatatt gtgtaattgt atctaaagcc gttgtcggct
    12841 catcagcaat gattaacttt ggtttcaaag ctaacgctaa agcaatcatc aatcgctgta
    12901 acattcctcc tgataacatg taagggtatg attttaatat acgtttagga tctttcaaac
    12961 ttaaataatc catatattca atcaatgtct tttcaatttc ttgtgtagac attgacgtat
    13021 gtactttcat agtctcaaac atttgtttac cgacagtagt tgatgggtca aaggcacgac
    13081 taccttgttg catgaccatc gcaatgtctt taccacggta ctttttcaat tgcgattcag
    13141 ataatgacaa cattgatgta ccatcaaaga taatttcacc tgtcaccccg agtcgttcgg
    13201 gattcaaacc aataatcgat ttacaagtga ttgatttacc actaccactt tctccaataa
    13261 cgcctaaagt ttcaccctta gttaatgtaa aattcacatc actcacgagt ggttgatctg
    13321 tccaggtatc tgtaatcgtc aaatgtttaa ctgttaacaa tgtcatgatt gcaccactcc
    13381 ttttttcaca gaacgaagtt tatctttaga agagatgcgg ggatcaatag caatttgtaa
    13441 agcatcggat aagaagttaa atgccatcac tataatcact atggcaatac ctggcgcaaa
    13501 catcatttca ggatgtgtaa acatcacttt tctagcttcg ttaagcatca tgccccactc
    13561 tgcagtaggc gctttgacac ctaatcctaa aaatgaaaag ccagatattt gcaagatcat
    13621 tgaacacatc gagctactag agatgatagc aatatctgct aatgttaatg gcataatatg
    13681 tttgtgaata attttcatat cattcatacc gattgtttta gcaaatctta catggtcaga
    13741 agcagtgtac tgcataacac ttgtacgtat aacacgacag aaccatgccc aacgcgtcaa
    13801 aataaatgcc atgataatat tttcggcacc cattccaaac aatgcaatta atgctaacgt
    13861 tacaacataa cttgggaatg ccaacataac atcacacgca cgcatgatta aggcgtcaac
    13921 aaacccttgg aaatatcctg ataagaatcc taaaatagat ccaataagta cagaaacaaa
    13981 tagcgcaaca aagacatata acaaacttgg tctaatcgca taaattaacc tagttaaaat
    14041 atctctacct aaatggtcag tacctagtag atgttgaaaa ctcatgccag caaatttgtt
    14101 tgctgtatcg atatggttag gatcataaaa tgtcacaagt ggtgctgcta atcctaaaaa
    14161 gacatataat acaataatgc ctaaagcaat tactgcacct ttatcttgta ataatcgttt
    14221 taaaattatc atcgtgcgcc ctcccttaat cttggattta atagcgcatt aatgatatct
    14281 gctaatgtat taaatacaat aaataatacc gctacaatta atacatatgc ttgaatgact
    14341 ggaaaatcgt gttcaagtat tgcttttaaa cttaattgac ctagtccagg ccatgcaaag
    14401 atatactcga taacaactag tccacccatt atcattggta tagacataca aaagattgat
    14461 accgcaactt gtaaagcatt acgcaacaca tgcaacatta atgtgataga tttcacaccg
    14521 cttgctctta aataaagtac ataatcttca tttaattgtt ccaccatcga gcgtctaaca
    14581 tttctaaagt aaataccagc ataggcaatc gtaataacga tcactggcaa tatgtaactt
    14641 tctggacctg ttaatccaga agtcggcaat atgtttaact tcactgaaac gtaaataata
    14701 agtattgaag ctatccaata tgatggtaat gcagttagaa agaaagccac tgaacgtatc
    14761 gcacgatcag tgaactttcc tctttttaat gcactaacta cacctaaaat aattgatgta
    14821 atcatcacca taacacttga aattattgtt aatttcaatg tattcataaa tgctggacca
    14881 atacgttcag caactgggtc acctgtaatg tagcttgtac caaaattaaa ttgcatcgct
    14941 tcaagtaacc aatttttata ttgaattaat aatggatcat tgaaaccgta cttctcattc
    15001 gtttctgcaa tcaactctgg tgttacattt ggcgtccctt gtgcatgtaa aattgtcaca
    15061 gctggatttt catttgtaat atacgtcaat agaaatgtca taaaacttac tacaatcatc
    15121 aatggaaaca tgagcgcaat acgttttaag ataaatttga acatctaatt gctcctttat
    15181 ttatactgca tttcattgaa tggtaattca tactgtgatt gtgtgaatga tactttttct
    15241 aaatcttttg gtgcaacaac tgtcatacta ccgtgtgaaa taaggataaa gataccttca
    15301 tcatcaattt gtttcaaaat gtttttataa gcgtctgaac gctctttacc gttttggatt
    15361 ttaaatgcgt catcaatgct gttgtatatt ttatctttgt tctcaatgcc tgatgttgca
    15421 ctttcataac catttttcgc tttaaatgct gcaatagtac tttgtggatc gtacaataat
    15481 ccccaagttt ggttgaacat taagtcataa tcaccagaag tacgacgttc agcaatttta
    15541 tctgatgttt cgccattgat gtttaactta atacccattt tcttaaattc tgcttgtaag
    15601 tattctgctt gttctttttg acttgaagaa cctttgtcat agtacattgc catttcaagg
    15661 tttttaccat ctttttgacg aacatcgctg tctttacctt tcttccaacc agcttcatct
    15721 aataatgatt ctgctttttt aaggtcatac ttacgtgttg gcatatcgaa attaatgtct
    15781 gttacatttt tcgcaaataa ttgagttgct ggtttttctt gaccatctaa aatttctttg
    15841 gcaattttat ctctgtttac catatgacca atcgcttgtc tgactgtttt gtcactcaca
    15901 gcgttatctt ttttaccaga attgacaact aacattttcg tattcatagg ttgactacgc
    15961 ttaacttgat agtcacctgt atctttcaat tgttttaaag agtctttgtc taagctatct
    16021 gtacctctat catctgtgaa ggcaaagttc gtttcacctt ttttcattga taggaatgct
    16081 gtttcaccag caggcattac ttttgcttgt actttgttaa gtttagactt ttcgccccag
    16141 tattgatcat ttttgttaaa gtctgcagac tcatcttttt tgtgttcacc taatttaaat
    16201 ggaccagtac catcgaactt tttaacgcca tcttttgttg taccgttttt aaagtctttt
    16261 ggagacacaa atacatatgg acgaggcatc gctaattcag ccaatgcagg ttgatatgct
    16321 tctttcaaat tcaattcaac cgtgtactta tctttaactt taacattgtc aattaatgtc
    16381 gaaatcttta accaagaatg caattttttg ttttcttgaa ctgcgtcaat atttttctta
    16441 actgcgtcag catcaaatgg cgtaccatca tggaatttaa cgtcatctct caaatggaac
    16501 gtgtatgtct tcccatcttc agacacatcc cactttttag ctagtaaagg cttaatacca
    16561 tctttcgtgt tacgtacaag cggctcgtat atcatacttt cagcagacat tgatccaccg
    16621 taaacatgcg gattcatatc accgatatct ttaaccgtcg tatacgttaa ttgcttgttt
    16681 tcttttttct cctctaaacc tttattaccg ccacaaccag ttaaaattag ccctgatgca
    16741 agtaacattg cactcatttt agttagtttt ctcatttgct tttcctcttt ctaaattgat
    16801 aagttgcatt acttaatcaa atcgtaatga ttatttttct caattatttg cttattttta
    16861 tgtaaatgtc aaacataatt caataatttt tttctattat aaaacttcaa acccttatta
    16921 tgaaagcgtt ctattgattt ccaaaaattt tgtgacgagt gctttatctc cttcaaatag
    16981 atttgtatta aattgagaag ataagtgttg atcttgatgc atatccttgt acgcctggca
    17041 acttgcttca tagcgagtta ggaactgatc aatcattggc gttttgatac ctagcatttt
    17101 cccaatatgc tgaatcatcg ccgttctgta ataatcttca cttggcattc ttggaatttg
    17161 aacaacatcc tgttcatttt tatagacttg cttaaatggt acagctgaaa aatcaaagta
    17221 atgtccgttt tcgtctggct gtgaaaatgg atcaatgagg attgcggtat atcttacata
    17281 aagcagatat tcttgtaaga tatctggcaa gatttcgaaa tgctcaatat caccttcatc
    17341 caaagtttca ggacgtactg gataattttc cttcaccata aattgaagca ggttgactga
    17401 cggcactcta aatgcttgta aaataaccat catttccttc cacattaaac gcatttcacg
    17461 gattagtgtc atcgttatcg gtccttcagg aaataactta tacacataaa ccggtacatc
    17521 tgttccttcg aaaatggctt tcaatgaaaa gtcattcata aatagtggtg ggtgcacata
    17581 aagcgaactg tttcgcgttt cagcatgcag tggcgattca acgacttcta attgaatctt
    17641 caattgctca gctaaagcag agattcgttg acacattgtt gagtttgaat gtgtcgatcc
    17701 catgtacaat ttctttttta cacctgttgt caacacatga ttaggcgctt ctttatcaac
    17761 aatacgtgta tcgccaagat aagttgagaa tgaaatcact tcgatatctt tattaaattt
    17821 agacataaat tgttcgacaa tcatttgcga accaaatgtc ggtgatatta aaatgacatg
    17881 tttgacactt tgcaaagttt ctaacgacaa ttgctgtagt gtgtcataat aagcatctgc
    17941 tgtgcatgcc atgacaaccg tttcgtattc acccttaacg tttttaacat ctttatacaa
    18001 acgattaatt tcaaacttac cttccagatg ttgatgcgcc tcgttttgta ttttgacttc
    18061 aaattgtttc gcttttttat acgcttgata taagcgtttt gatttttctg atgttgaggc
    18121 acgtccaacc atatcaatct cataatctga ttttaaatag caaatattcg ctaattggat
    18181 tgcgaccgga ccagtgccta tcattaataa tttagacatc cgtaatacct acctttatag
    18241 ctgctttttt ataaagtgct atatcaaaaa tttgctgtgg tctcatatgt ttattcacac
    18301 attgccactt atcttccgct gtttcttgtg acggataatt aaatattgct ttgatgccat
    18361 caccaaagcg cattgcaact acgacatttt catttgttaa atcatataat tcttctaaaa
    18421 tgctgtactt taaaggaatt gtcgagctga atatgatatg cgtcacatct ttgatatctt
    18481 taagttcaga taccttttga tccgtaattg ttatatcttc atttggtgct aagacgttaa
    18541 cgattctgcg ccctaggtca acggcttgtg gatcaatatc aataccgata actgaagcac
    18601 ctgtttcttt tgctacttga attaacgtca ttggatatgc acctgaacca actaacaata
    18661 atttgtcatt gctagctata ctacattgtc caaactcttc atcaatacaa tgttctatat
    18721 tgtcaaagta acctgtggta gacgcctgtc cgtctaatag acgtctcgct ctaatgactt
    18781 ccacttgttt aacacactgc gctgtaatat attgcagtga ttttattaat tgtgcttttt
    18841 cttcaacgtc acgccaagca ttaaattgtt gttcataaat aggatttaaa ataaattcac
    18901 tatagtcatc catcaatgtt tctaatgctt cgatatattg atcgtcttgt aatacacgct
    18961 cgtaatgcgc ttcaaacttt tctaaatatt gttgtaatat caatttgatt tcattattaa
    19021 agttattcat tgtaggctcc ttattctata tatgcttttc ctgtagctac agttgtaacc
    19081 tgtcctttaa ttgaagtttg atatcccaat tgatgacatc gctttgatgt cactaaaata
    19141 ctgccccctg gctgatgtac tgtaaaatct ttgcatgcgt cattacgttg ataattatta
    19201 aaaaccccaa ttgatgctgt accagaacca cagctatttt cccaaattaa actttgaatt
    19261 tctggtatat agattaatgg ctgtaaaaat tgacgttgtt catcaaaaag catcatacct
    19321 actgttttat atttgtgact ccattgttgc tcacgcacaa acgcttcaac caaatgttga
    19381 atttctgttg ttacttgttt aactggaatc acataatgta catatgtttc ataaataatt
    19441 tctattgctt tccatgaatg attacccata ttaattgttg ttggcacaac acgatgggct
    19501 tgtggcattt gaacttcata gtattggcaa tcatgaattg cgcattgcac taaatccgaa
    19561 cagccagaca ccttcacctt aaactgttgg tctttaagca aatgactttc ctgcaaatga
    19621 tgtatatatg acatcgtcgc attaccgcaa aattcattac cgctcataac taagtgaaaa
    19681 tcattaccat catcattttg tgttgattct ataaagccta cctgttcaca gcatacatgt
    19741 gttgcggcca tcaactgatt ggcgatagat gcatattcac tagcatcatg ttttgaatga
    19801 acaagtatcg tcatattccc cgaaggatta tacttagaaa attctataac ctgccgattc
    19861 atacgacact cctttagatg tatttttaga gcctctgtaa ttttaattac tagaggctct
    19921 tatgcagttg ttgcgaagca aacaactgta ttcctctaaa cctgtaattt tttagaagat
    19981 gaacctaata gctctatcta gtaggtaagt catgctaatt cgcaaaagcg aaaaccatgg
    20041 ctcttatgca gttgttgcga agcaagcaac tgtattcctc taaaactgta attttttaga
    20101 agctgaacct aatagctcta tctattaggt aagtcatgct aacccgcaaa gtggaaagca
    20161 tgacttagta atcggttgtt gtatcaaaat gcaaccgtaa accttattta aagttgcttc
    20221 ttgtggctct acaagcgaat gcagttgtcc gttaactaca ttcacttaga tgcttttact
    20281 gttttcacct caactaaaac ttcaaaaaag ttgcagaaaa caaatcgtaa ctattacgat
    20341 ttgattatat actatattag ttgagaatca ttgtcaataa aggttgtaaa tgaatgcaca
    20401 tttatatttt gataatcctt ccataaactt cgtattctca gcacattcaa gtctttttga
    20461 aaatttttat caataattga gtagaagttc cgttctagtc agttcaaaat tttacgtaag
    20521 ttgtctctgc gtattgcttt agtgagaata acccacactt gtggaaacgt ccttttgtta
    20581 atctacaatg gatacccttg tctttcgcta acatttccta ttaccaatcc tgtaacggat
    20641 tttcatcgtc aagttgttgc ccatacaata cgcaccaaaa aagctttgta caatttcgta
    20701 caaagcttta aaaatttaaa taaaaatatc attgttttaa tgaactagcc atatcagatt
    20761 ttatttcgtt tgctaatact tgtaattcag ggaagtaatt attagcaaag ccaattatta
    20821 ttttattttt tacaaacgtg ttaatctctc gatcttctgg aatcgtattt tctattaaat
    20881 tcaaaagctc actatgactt atgttctcta tacttactgt atttaaatat tttaaaatca
    20941 caagatacaa ttcatcaaat acttcatgtt caaaatcaaa gtccaataat aataaactaa
    21001 cggaattagt ttctaactga ttatctccaa ttttatctgc aatctctctt atagcttgat
    21061 atagcaatct aaaactttct ttttctttta acataaaatt ctccttaatt tattagaata
    21121 gataaaatac agcttatttt aaagatactc caaaagtttg aactcgagat tctgataaat
    21181 tagatgcacg acctacaata cgttaaaaca agctttaagc aatacacttc aaatgaacaa
    21241 tatgtgattt tcaaagatta attaatctcc gctttaaatg tctttcattg cgacaatatg
    21301 catacattgc ctacttcatg ccaaattctt ttccactgga tatcgatttt aaataattat
    21361 tccaatgtcc gcttccacga atcatcactt aacatctcgc caggccatgt gtaagccatc
    21421 acaccaccat caattcgaat cgtctctcca gttatgaatg aactgtcgtc agatgctaag
    21481 aagactacta atttagcaac ttcttctggt ttacctaaac gtcccagcgg agtcatccat
    21541 ttttgatttt ctctaaatgt tttacctgca tcatcctcac tcgtacctgt cagtttatct
    21601 actaacgggg tttcaattgt acctggtgca atcgcattgg ctcgaatgct atcacggcca
    21661 tactcaattg cgattgattt tgtaaaatta atcactgcac ctttcgcagc attatatcca
    21721 gagcgataca agtctgctgc ttgtccggaa aatgaagacg tattaacaat agagccacct
    21781 tgattcatca ttaaaggtaa catcattttc gtcattaaaa atgtcccacg catatctaca
    21841 ttcataatct tgtcatacac atctattgga tactcatgaa ttctatcagc cgcattatcc
    21901 acaccggcat tattaaacaa cacatcgatt cttccaaact gttcctttat gtcagacaca
    21961 aagtctacca cttgttgttc gcttgcatta tccacattat acgccttcgc attgtcacca
    22021 ttacttttaa ttttatcgac agtctccgat accgcttcag ctatgtctac cgccaataca
    22081 tacgcacctt attgagctaa agcgattgca gaagcttgac cgatacctgt acttgctcct
    22141 gttacgactg ctactttatt ttccaaacgt ttcattcagt cactcctata caacatatct
    22201 taaacttaca gtccttattt tccactgttc gatgcttcat aaacctaata gataagtctt
    22261 caggcagtct taactactct gctatacact ttctattgaa taactcaaaa tatttaacaa
    22321 tattagtttt tttattttct gaaaattgtt acttttgaga gtattttttg ttacacttta
    22381 aatatttaac aaagggggga ataattgatg acatcaaaac atcaacaaaa ggggtctatc
    22441 gtcaatcgtt tcttgaatag tgttgaaaaa atcggaaata aattgccaga tcctagcgtc
    22501 ttattctttt taatgtgtgt gggcttagcc attatgacat gggttatctc attatttaat
    22561 gtatctgtta agcatccagg tacgcatcaa accatttata ttaaaaatat aattagccat
    22621 gatggattta cgatgataat gaatgatacg attaaaaatt tctcagagtt cccagcatta
    22681 ggcttagtac tagcagtgat gattggtatt ggtgttgcag agaaaacagg atacttcgat
    22741 aagttaatga tttctgttgt gaatcgcgca ccacgcttct taattttacc gactattata
    22801 ttaattggta ttttaggtag tacagccggc gatgctgcga caattatctt gccgccgctt
    22861 gcagcaatgc tttttattaa aattggctat caccctatcg ctggactaac gatggcatat
    22921 gcttccgctg ttggaggatt tgcagcaaat atagttgttg gtatgcaaga tgctttggtc
    22981 tattcattta cagaaccggc aacacgtatc gtttcagatt ctattaaaac aaacgttgct
    23041 atgaactggt actttatcgc agcgagtgtc gttgtattac ttcctacgat attgctagtt
    23101 acaactaagc tcattattcc tagattagga aaatatgatg acagtttaat gcatgatgac
    23161 catgaagaaa catcttcaca tattactgac aaagaagcac atgctttaaa atgggcaaat
    23221 atcagtttca tagtcacaat tattttatta attattacag ccattcctga acatagcttt
    23281 ttaagaaatg ctaaaacagg cagcttacta gacgatgcgc cattaattaa cggtgtcgga
    23341 ttaattattc ttgtcgtatt tttagtacct ggattagttt atgggatttt aagcaaagaa
    23401 attaaaaata caaaagactt aggtaaaatg ttcggcgatg ctgtaggatc aatgggaaca
    23461 tttatcgtta ttgtgttttt tgcagcacag ctacttgcgt atttaaagtg gagtaattta
    23521 ggaattatcg ctgctgttaa aggtgccaaa ttattagaac atcaaaacgg tattgtattg
    23581 atactaggta ttattgtact gagtgcaatg gtcaacatgt taatcggaag cgcatcggct
    23641 aaatggggta ttttaggacc gatattcgta ccaatgttaa tccttatcgg ctttcatccc
    23701 gcattcacac aagtcattta ccgtgtaggc gattcaatta ccaatccaat tacaccgatg
    23761 atgccgtact tacctttatt attaacttat gcacaaaaat acgataaacg catgaaactc
    23821 ggagccttac tttctagttt aatgccgtat tcaatcgcgc taagtattgt atggacagta
    23881 tttgtcatca tttggttcct attaggtatc cctgttggac caggtggacc gatattcgtt
    23941 aaataacagt attcaaaggg ataaattaaa atgccactat acaagatttg acctgtatag
    24001 tggcatgatt tgttatgata tagcgttcga gatgaaactc tgcatttata tgaacaatta
    24061 tagtctatca ctaataatat ttaatctgtt ccaaccgtta atttggttta caacaaagac
    24121 aagatcaata atttgttctt catcataaaa tgattttagc cggtcaataa tttcaaactt
    24181 cttaaagtct ttgattgaat ttaacatttc tgcaaattca agcgtcactt tttcttgatc
    24241 agtaaataaa tctaaatgtc taaaactcaa gacttcatca attttcattt gtgttacacc
    24301 caattccttt aattcttttt tatggatatc aacacaataa tgacaaccgt taattttaga
    24361 aattaataac ttaatcaatg cactaagttc atcattaacg ggtgattgat gaataaactt
    24421 ttcaccttgg ctcattacag acattaactc tccattttgt ttcttgtaat tatacggcat
    24481 ttgcaatctc tcctttacat tgtttaatac caatccagtg atactacact tcgaacaata
    24541 ttggatagaa tttgaaattc tgtataacat cttgtcaatc tatgctttag ttaaaacatt
    24601 tagcacttat atttaaaaaa tatataaaat atgaaaacag attgaaagga tattatacct
    24661 tgctctaccc ttttttataa aaaatcacac acatttgaga aaatttgata atgtcaataa
    24721 atcacgtcat cgacattgtt attcgatata tattgttctt ataatggcca aactattatt
    24781 ccatttaaat taacgtggtt cgtttgtgtt aatgtactgt acaaaactgg ggaagtattg
    24841 gtgatgatga agtgatttat attaattgcg aaacaccttc gatagcaatg acatagatta
    24901 ttggaagatt aaatcaaaaa taaagaagac cctagtggcc ttctttttta tctaattctt
    24961 ctttgatttt ttctggtgat tttctttcat gtgctggttt taataattta tctactcctt
    25021 cactttttat catagcgcca ccaaattgat aattatgttc aggcgatgca taagcagtaa
    25081 aatcatcttc tttattttca ttgatgtatc cttcaatcac agcacttccc ataggacttg
    25141 tttccaagtt tgtgaaatgt acagatttaa tcgtgttcgt ttctttagta ttatgcttaa
    25201 gatagagtgt aatacgttct tgttgttctt tgtagtaaat cgctttttgt ttttcttttt
    25261 catcatattt cattttaaaa tgcatgaccg tagaaactgt acatatacac aatatgacac
    25321 caataattat aaaaacatgt tttttggaca ttaaagtaac tcctaacttt agtgactctt
    25381 ctttttattt agttcttttt ttatatcatc tggtgatttt aactgattgg ctggctttag
    25441 taactcgctt aatttttcac tttctatcat agcaccacca aattgataat tatgttctgg
    25501 tgtagcataa gcagtaaaat tatctttttt gctttcattt atgtaacctt caatcacagc
    25561 acttcccata ggacttgttt ccgagtttgt gaaatgtaca gatttgattg tattcggttc
    25621 tttagtgtta tgcttgagat aaagtgtaat gcgcgcttgt tgttctttat agtatattgc
    25681 tttttgtttt tctttttcgt catatttcac ttttaaatag atacctgcaa cactaattaa
    25741 tatgattaac ataatactag ttaatattaa aattttcttt ttcgacatct atcaccagct
    25801 cctaaaatgt ggtggaattt tatacagttt acatatttat gatactacaa aacacactct
    25861 tacaaagaaa atctataatt ttaatctagg tcaaggaaaa gcagccaaaa gtgtttatat
    25921 aagatcaacg aaatattgcg ggtataaagt ggttaacaga taaatacagt ggaaattgta
    25981 ctaaaaattc aacttttcat ttttatatgt aagtatgatt aacttgaaaa caaaattaaa
    26041 acggccgcat agttttagcg gtcgttcttt ttagactcta attccttttt tatctcatca
    26101 ggtgatttgg tttgatgtac tggttttaat aacgtgctta aatttttact tttgattaaa
    26161 cttccaccaa attgataatt atgttctgga gacccatatg caacaaaatc atcttcttta
    26221 ttttcattta tgtaaccttc aattactgca tcgcccatag gtcctctttt taaacttgtg
    26281 aaatgtacag ttttgatggt attgggttct ttagtattat gcttaagata gagtgtaata
    26341 cgctcctgtt gttcttt
//
LOCUS       DALAAR010000035        26309 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000035 DALAAR010000000
VERSION     DALAAR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.036"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(164..568)
                     /locus_tag="O2458_001489"
     CDS             complement(164..568)
                     /locus_tag="O2458_001489"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCZ3887355.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(588..2000)
                     /gene="atpD"
                     /locus_tag="O2458_001490"
     CDS             complement(588..2000)
                     /gene="atpD"
                     /locus_tag="O2458_001490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCZ3887356.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2022..2888)
                     /locus_tag="O2458_001491"
     CDS             complement(2022..2888)
                     /locus_tag="O2458_001491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCZ3887357.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(2919..4427)
                     /locus_tag="O2458_001492"
     CDS             complement(2919..4427)
                     /locus_tag="O2458_001492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCZ3887358.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4449..4988)
                     /locus_tag="O2458_001493"
     CDS             complement(4449..4988)
                     /locus_tag="O2458_001493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCZ3887359.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(4988..5509)
                     /locus_tag="O2458_001494"
     CDS             complement(4988..5509)
                     /locus_tag="O2458_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCZ3887360.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5707..5919)
                     /gene="atpE"
                     /locus_tag="O2458_001495"
     CDS             complement(5707..5919)
                     /gene="atpE"
                     /locus_tag="O2458_001495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCZ3887361.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(5962..6690)
                     /gene="atpB"
                     /locus_tag="O2458_001496"
     CDS             complement(5962..6690)
                     /gene="atpB"
                     /locus_tag="O2458_001496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCZ3887362.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6711..7163)
                     /locus_tag="O2458_001497"
     CDS             complement(6711..7163)
                     /locus_tag="O2458_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCZ3887363.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7231..8358)
                     /gene="wecB"
                     /locus_tag="O2458_001498"
     CDS             complement(7231..8358)
                     /gene="wecB"
                     /locus_tag="O2458_001498"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCZ3887364.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8382..9011)
                     /gene="upp"
                     /locus_tag="O2458_001499"
     CDS             complement(8382..9011)
                     /gene="upp"
                     /locus_tag="O2458_001499"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCZ3887365.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9039..10277)
                     /locus_tag="O2458_001500"
     CDS             complement(9039..10277)
                     /locus_tag="O2458_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCZ3887366.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10304..10828)
                     /locus_tag="O2458_001501"
     CDS             complement(10304..10828)
                     /locus_tag="O2458_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCZ3887367.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(10935..11354)
                     /locus_tag="O2458_001502"
     CDS             complement(10935..11354)
                     /locus_tag="O2458_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCZ3887368.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11351..12397)
                     /locus_tag="O2458_001503"
     CDS             complement(11351..12397)
                     /locus_tag="O2458_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCZ3887369.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12481..13317)
                     /gene="prmC"
                     /locus_tag="O2458_001504"
     CDS             complement(12481..13317)
                     /gene="prmC"
                     /locus_tag="O2458_001504"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCZ3887370.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13304..14380)
                     /gene="prfA"
                     /locus_tag="O2458_001505"
     CDS             complement(13304..14380)
                     /gene="prfA"
                     /locus_tag="O2458_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCZ3887371.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14381..14980)
                     /locus_tag="O2458_001506"
     CDS             complement(14381..14980)
                     /locus_tag="O2458_001506"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCZ3887372.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15327..15581)
                     /locus_tag="O2458_001507"
     CDS             complement(15327..15581)
                     /locus_tag="O2458_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCZ3887373.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15699..17015)
                     /gene="rho"
                     /locus_tag="O2458_001508"
     CDS             complement(15699..17015)
                     /gene="rho"
                     /locus_tag="O2458_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCZ3887374.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17262..18689)
                     /locus_tag="O2458_001509"
     CDS             complement(17262..18689)
                     /locus_tag="O2458_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ3887375.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(18927..19262)
                     /locus_tag="O2458_001510"
     CDS             complement(18927..19262)
                     /locus_tag="O2458_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887376.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(19351..20610)
                     /locus_tag="O2458_001511"
     CDS             complement(19351..20610)
                     /locus_tag="O2458_001511"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ3887377.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(21076..21936)
                     /locus_tag="O2458_001512"
     CDS             complement(21076..21936)
                     /locus_tag="O2458_001512"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCZ3887378.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            22154..22675
                     /locus_tag="O2458_001513"
     CDS             22154..22675
                     /locus_tag="O2458_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCZ3887379.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(22784..24394)
                     /locus_tag="O2458_001514"
     CDS             complement(22784..24394)
                     /locus_tag="O2458_001514"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCZ3887380.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(24730..25260)
                     /locus_tag="O2458_001515"
     CDS             complement(24730..25260)
                     /locus_tag="O2458_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCZ3887381.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(25372..26232)
                     /locus_tag="O2458_001516"
     CDS             complement(25372..26232)
                     /locus_tag="O2458_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887382.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT         7885 a         5242 c         3807 g         9375 t
ORIGIN      
        1 ttaaatacac attagctgtg tttaatgata aagaaccact acatcataaa tctttaatgg
       61 ttctttatca attccttccc ccctctcatg ttttgtatct ttaaatcaat ataattcaaa
      121 aaagggtcga agatatgaaa tcacatcttc gaccctttat ttactatttt aattcagcca
      181 cacgcaaacg gttatttgct ctctctaaag ctctttcggc tctatgaata tcagtattgt
      241 cgtcatcatt ttccaagtga gactctgctc ttgctttggc taatttagct ctttcaacat
      301 caatttctct tgcagtttct gcagtctgaa caatgattga aactttatct tttctaactt
      361 caacaaagcc atcgcttaca gcaatatatt cagttccatc gtgaaatttc acttttacaa
      421 agcctgtttt taaagcagct acagttggaa tatgtccact catgacacct atctcaccag
      481 ctgttgtttg cataacaacg agttcaacat tatcacgatt gtaaacagaa ccattaggag
      541 tgacaatatc taggtttaat gtattcatta tccattcctc ctaattgtta tacttcaaca
      601 cccatatctt tagcttttgc aataacatca tccatgctac caactaaacg gaatgcatct
      661 tctggaatat ggtcatattt accatctaag atatctttaa agtttgcaac tgttgtctta
      721 acaggtacat aagaaccttt ttgaccagta aattgttccg ctacgtggaa gttttgagat
      781 aagaagaatt gaattctacg tgcgcgttca actgtttgtt tatcttcatc agataattcg
      841 tccataccta agatagcaat gatatcttgt aattcacggt atttttgaag tgttgattgt
      901 acatcacgag ctacttcata atgttcttga cctacaattg atggttccaa tgctcttgat
      961 gtagacgcta atggatccac ggctggataa atacccattt cagttaattt acgttctaag
     1021 tttgtagttg catctaaatg ggcaaacgct gtcgcaggcg ctgggtcagt atagtcatcg
     1081 gcaggtacga ataccgcttg aatagaagta actgatcctt ttgttgtaga cgtaatacgt
     1141 tcttgtaatt gtcccatttc agtagcaagt gttggttggt aacctactgc agaaggcata
     1201 cgacctaata atgcagatac ctcagaacca gcttgtgtaa atctgaaaat gttatcgatg
     1261 aataataata cgtcttgacc ttgttcgtca cggaaatatt cagccattgt taaaccagat
     1321 aatgcaacac gcatacgtgc accaggtggc tcattcattt gcccgaatac catggctgtt
     1381 ttcttaatta caccactgtc actcatttcg aagtataaat cgttaccttc acgagtacgt
     1441 tcacctacac cggcgaatac agaaatacca ccgtgctctt gagcgatgtt gttaattaat
     1501 tcttggatta atactgtttt acctacaccg gcaccaccga acaatccgat tttaccacct
     1561 ttaatataag gtgctagtaa atctactact ttaatacctg tttctaaaat ttgaacttct
     1621 gttgaaagtt catcgaatgc tggtgcttga cgatggatag gatcgcggcg aacagaatca
     1681 ctaatttctt ctttaaggtc aattgtttca cctagtacat taaatacacg acctaatgtt
     1741 tcgtcaccaa caggtacact aatttctttg cctgtatctt ttacatccat gcctctttgg
     1801 acaccatcag ttgaatccat cgcaattgta cgaacaacgt cgtcacctaa ttgcagcgca
     1861 acttctaatg ttagttgtat tgtaccttct tctttaggca catcaataac caaggcgtta
     1921 ttaattttag gaacttcgtt atgttcaaat cgaacatcaa ttacaggacc cataacttga
     1981 gttacacggc caattcccat gctattttcc tcctttaaat attattcaag cgctgcggaa
     2041 ccaccaacaa tttcagtaat ttgttgcgta atttctgctt gtctcgctct gttatattct
     2101 aatgataagt catcaataag ttcagttgca ttatcagtgg catttttcat cgcagtcata
     2161 cgtgttgcat gctcacttgc ttttgcgtct aatattgttc cgtaaatcaa actctcaaca
     2221 tattgaggca agattacact taagatagat tctttatctg gctcaaattc ataagaagac
     2281 aaatgaccat gccccttact agaatcctct tgagataatg gtaatacttg tctagatgta
     2341 ggcttgtttt caagaacgct gacataatga ctatagtata tatttaattc atcaatttct
     2401 tcttcactgt ataagtctat agcatggtta gctagtgctt gaacagattt gaaagaaggt
     2461 tgatcaggta cgtctacttg agaatactca atgtcataac ctctattttt taggaaatca
     2521 acaccttgtt gccctaaaac tacaatactg tattcgctac tatcttgatg tttcgcttca
     2581 atatcagtaa tcaatttttt aagcacgttt gcactatatg cacctgctaa acctttatca
     2641 ctcgtgataa ctaaatatcc acttctagta attttacgag gtcttagcat tggatggttt
     2701 gtattgctac ttgcccctgc aactgcagta attgcatctt gcattttatc catatatggt
     2761 gtgaattgtt ttgtattttt ttcagctcta cgaagttttg aacttgatac catgttcatc
     2821 gctttcgtaa tctgcttcat ttttttggtt gattttattc gagtatctat ttctttaaga
     2881 gaagccacta tctcaccacc ttatactaaa cttgtttatt attcagattt gctaaagctt
     2941 tttttgaatt cgttaattgc tgtgtcaaac ttctcagcat ctggtaagcc accagtttct
     3001 ctgatttcat ttaacagttc agtagcattt gattctgccc agtggtttaa ctcgtcttca
     3061 aaacgtgtga tatctacaac tggaatatca tctaaatatc cttttgttaa tgcataaata
     3121 atcaacactt ggtgttcgac tggtaatggt ttgttttgat cttgttttaa gacttcaaca
     3181 gtacgtttac cacgttctaa tttacttgca gtaaattcat caaggtctga accgaattgt
     3241 gcaaatgatt caagttctct gtatgacgct aagtcaagac gtaacgtacc agcaactttc
     3301 ttcattgctt taatttgtgc agatccacca acacgagata cagattgtcc ggcattaatc
     3361 gctggtctta cacctgagaa gaataaatca gattgtaaga agatttgtcc atctgtaatt
     3421 gaaataacgt ttgttggtac ataagctgaa atatcaccag cttgtgtttc gataattggt
     3481 aatgcagtaa ttgaaccgcc acctaagtca tcgtttaatt ttgctgctct ttctaataat
     3541 ctactatgta agtagaatac gtcacctggg tatgcttcac gacctggagg tctacgtaat
     3601 aataatgata actcacgata agctgctgct tgtttagtta aatcatcata aacgattaaa
     3661 acatgtttac cgttaaacat gaattcttca cccattgtta cacctgaata tggtgcaata
     3721 tataataatg gagaaggttc agaagctgat gctgctacaa caatagtgta gtctaaagcg
     3781 cctgcttgtc ttaacttttc aacatttgct cttactgttg aatctttttg accaatagca
     3841 acatagatac aaatcgtacc ttgatctttt tggttcaaaa ttgtgtcaat tgcaattgtt
     3901 gttttacctg tttgacggtc accgatgatt aactcacgtt gacctctacc aattggtact
     3961 aaagcatcaa ttgctttgat acctgtttgt aatggctcat ctactgattt acgatccatt
     4021 acaccagtag cttttttctc tactggacgt gttttagttg tgttaatcgg tccttgtcca
     4081 tcaataggtt gtcctaatgg attaacaact cttccgatta gttcttcacc tactggtact
     4141 tccatgatac gaccagtacg tttaacttcg tcaccttcag taatacctgt gtatggtcct
     4201 aaaataacca cacccacgtt tgactcttca aggttttggg ctaaaccaag tacgccgtta
     4261 tggaattcta ctagctcacc agccataacg tcatttaatc cgtgaattaa tgcaatacca
     4321 tcaccaattt gtaatacagt acctacatca gttacggaca tttctgactc ataattttca
     4381 atttgtgagc gaagtaatgc actgatttct tcagctttta tggccatcta tgtcactcct
     4441 ctttataatt aattaactct tctaaatttt ctttgtaatt ggacaagatc atttctaaca
     4501 ctaccatcta atacagttgt gccgacttta actctaaatc caccaattaa atctggatta
     4561 atttttgtat ctacaataac tttagataac ttcgtttgtt gagttactag tttgacaatc
     4621 ttatctaact cttcttgact caattcatat gttgactcaa ttgttgcaaa atcttgattg
     4681 tagtgtccgt tatataagct ttggaacgcc ttgaatacgt cagctattaa tgagatatgt
     4741 ctattatctg ctaacacgta catcatattt ttaatgtatg gattaatgtc agtaaataca
     4801 ccattaatca actcacgacg ttgttctgct gtttgagttg gattactgtc aaccattctc
     4861 aattgctcaa ttttatcttt tactgcttca tttataactg ttaattcttc attaatagtc
     4921 tctaaattat ttgtatctaa tgacacgtca aataatgctt tagcatactt gttagctact
     4981 tttaccatta tttatcgcct gcctctttta gatacttgtc aaccaatgct ttttggtctt
     5041 gttcagaaat ttcttttcta agaactttag aagcaattaa cactgatagt tcagatactt
     5101 gattattaat atctgcaatg gcacgttctt tttggctatt gatttcactt tgtgctgttt
     5161 caatcatacc gtttgcacgt acgtttgctt catgaataat ttgttcttgc tgttgacgtg
     5221 cttgaacctt agcatcttct aaaatctttt gaacttcttc ttgtgtttct ttaagttttt
     5281 gtttattttc ttcttcaagt ttctgtgcat ttaacttagc ttgttctgcg tcatcgatat
     5341 ctctgttaat atctctttca cgtttatcca ttacatcttt caatggaccc catgcgaact
     5401 ttttaagtaa cgcaagtaac acgatgaaag ttaggacctg tacaatcaca gtaccccact
     5461 caacgcctcc agctgcacca agaacgaata agttagctgt ttcagtcacg ggttcatgac
     5521 actcctttca ttaaaataat cgctagtgtt atatagaaaa aaacgacgag gttaggacct
     5581 actgaattta tgcgataggc atggtgcgtt catatctcaa ctatttaata aagttcaaag
     5641 atattactat atatgcatca tgaattatgt cctatgcgct ttgtcccgac ctcttttatc
     5701 tgttaattat ccagcaaatg tcatgaatgc aattactaca ccgatgatag gtaatgcctc
     5761 aactaaacct acaccaatga acatgatacc cattaattga ccacgtgctt ctggttgacg
     5821 tgctacacct tcaactgttc ttgaaacgat taaaccgtta ccgatacctg ctcctaatgc
     5881 tgataaacca attgcgattg ctgctgcgat taaattcata atttaatttc ctcctgtgat
     5941 tgtatataat tattgaaatt tttagtgttc atctgccact ttatgtgaca tataaaccat
     6001 cgaaagcata ataaagatat atgcttggat tgttcctaca aatattgaaa atgcttgcca
     6061 aacaattaat cctgggatac taataatcca accccatgct ggttcgttaa agaataagcc
     6121 agcaagtaat gttaatagta tctcacctgc aaatatgtta ccgtacaaac gcagaccaag
     6181 cgttaatgtt gaagtgaact cttcaaaaac attaataatt gccaatggcc aaaacggctg
     6241 tacataacct ttaagatatt gtttcgtacc acgcatttta attccataaa agtgagttaa
     6301 cagtattatc gttgtagaca acgttaaagt cactgttgca tcagctgtcg gtgatttcca
     6361 ccacaatgtg tgatctttcg ttactataga aaacggaaga cctaacatat tagctataaa
     6421 aatgtacaga atcagcgtta ctgctaagaa gtggaattga ccaccttttt tccaagccat
     6481 gttaccttca atgattcccc tcacgaaatc aaaaatccat tcaacgaaat tttgtttgcc
     6541 agttggtctt tttttcaaat tacgcgtaca aatgatagca agtagaaaaa caagaaacgc
     6601 cgtaacaagt atcatcaata tacttgataa attgaaaacg atatcaaaac cgaataaatt
     6661 ccaactcacg agcggggatt tgtgatccat aatctcacct ctttcttaat ttatttgctc
     6721 acctttagta aaggacgtat gataactaca acatatgaaa tcattaaacc aataattata
     6781 ccgatgatac ttacatgcgc tttattaagg taccaaatca tacaggcaat aattgcaact
     6841 aagtatcgcc acatatttcc agttgaaata tgcatagtgt ctggtctttt agctttagct
     6901 aaatagcatt caaaaataca tgtgttgatt gcagaaccag taacaccaat gattaatcct
     6961 aagataaatg catggtgtgt ataaaggtat aatcctccca atattattat tagataaaaa
     7021 taatattgaa tgaactgttt aaaaatgttg tagaaacgac tcattttata tgcctccgtg
     7081 tgactgatat caatcatgaa aaccgttcca ctcatacaaa ctaaatgata atataggcga
     7141 tttgtggtgt caattctatt caaattgttg taaaaaaata agcatagtta gacacaattt
     7201 agacacaatt agacaaaaag tctattttcc tcagaaatcg cttggttttt cagtgattaa
     7261 attcaaataa tacttaatat gattaactat tctttctgaa gcaaatccat caccgtaagg
     7321 attagatgct tcagacattt gatgatataa tctctcatca tcgattaatt cttttgcagc
     7381 ttgatacaca ttttgcttat ttgttccaat gacttttaat gtgccagctt caacaccttc
     7441 aggacgttct gtaacacttc gcaaaactaa aactggctta ttaaatgatg gcgcttcttc
     7501 ctgaattcca cctgaatctg tcaaaataaa ataagatttt ttagcaaaat tatggaaatc
     7561 tactacgtcc aaaggttcaa tcaattcaat tctgtcatga ctacctaaaa tcttttgagc
     7621 cacctctcga actttcgggt ttttatgcat tggatatacc agtgctaaat cagtatactc
     7681 atctattaag cgtctaaccg ctttaaatat attttccatg ggtttcccga tattttctcg
     7741 tcggtgtgct gtcataagaa tgaatttttt gtcatggtat ttatccatga tgttagattt
     7801 ataattgtca tcaactgtat atttcatagc atcaatcgca gtattaccag tgacaacaac
     7861 actttctgaa tatttccctt cacttaacaa atgcgatgca gcatttttag taggtgcaaa
     7921 atgtaagtca gctaatacac caactaattg tctattcacc tcttctggaa aaggtgaata
     7981 tttatcataa cttctaagcc ctgcttcaac gtgtccaatc ggcacttggt tataaaatgc
     8041 cgctaaacca cctgcaaatg tcgtcatcgt atcaccatgt acaagtacca tgtctggttt
     8101 ttctaattga atcacttgtt ctaattgagt gattgattta gaagttatct cagaaagtgt
     8161 ctgtcctgat ttcataatat tcaaatcgta ttttggtttg atttcaaagg tacttaatac
     8221 tgaatcaagc atttctctat gctgtgctgt aacaacaaca attggctcga gcattttttc
     8281 ttgttccaaa gctttaacta aaggagccat ctttatggct tcgggtcttg ttccaaatat
     8341 ggtcataatc tttttcatca aactacttat ctccgattct tctatttagt accaaacaat
     8401 ctatctccag cgtcgcctaa ccctggtgtg atatatgctt tgtcatttag cttttcatca
     8461 agtgcagcaa tataaatatc tacatctgga tgtgcttcat gcatcttttc tacgccttct
     8521 ggtgctgcaa ttaaacacat gaagcgaata tttttagcgc cacgtttctt caatgaagta
     8581 atagcttcaa ttgctgatgc gcctgttgct aacataggat caacaacaat gatttgtctt
     8641 tcagtaatat cttgaggtaa cttagcaaaa tactctacag cctttaatgt ttcgggatct
     8701 cgatataaac cgatatgtcc aactctggct gcaggtacta aacttaaaat accatcagtc
     8761 atacctaaac cagctcttaa aattggaacg atagctaatt ttttaccagc taatcgttta
     8821 gccgtcattt tagttacagg cgtttcaata tcaacatcct gaagctctaa gtctctagtt
     8881 acttcatatg ccatcaacat accaacttcg tctacaagtt ctctaaattc tttagtacct
     8941 gtatttacat ctctaatata gcttagtttg tgttgaatta atggatgatc gaaaacgtgt
     9001 actttactca taaaaattac tcctatcttt gtgtatgttt attgatatag aggatattca
     9061 gctgttaatt tcgcaacgcg ttctttagct tgttgtaatt tttcttcatc tttactattt
     9121 ttcaatgcta aactgatgat ttttgcaact tcctcaaaag ctttttcatc aaatccacgc
     9181 gttgttgcag caggtgtacc taaacgtata ccactcgtta caaaaggttt ttcttgatcg
     9241 aacggaatgg tatttttgtt acatgtgata ccaactgaat ctaaagtctc ttcagcttct
     9301 ttaccagtaa gtcctataga cccttttaca tcaacagcta ctaagtgatt atctgtaccg
     9361 ccagaaacaa ttctaaatcc ttcattaatt aatgcttctg caagaacttt tgcgttttta
     9421 accacttgtt gttgatacgt tttgaaatta ttttctaacg cttctccaaa agcaactgct
     9481 tttgctgcaa taacatgctc aagaggtcca ccttgaatac cagggaaaat tgttttatct
     9541 atgtcttttt tatattcttc cttacataaa atcataccac cacgtggtcc gcgtaatgtt
     9601 ttgtgtgttg tagttgttac aaaatcagca tattctactg gatttggatg taaacctgcc
     9661 gctactaatc ctgcaatatg tgccatgtct accattaact tagcgtttac ttcatctgcg
     9721 atttctttaa actttttgaa gtcaattgtt cttgaatatg ctgatgctcc tgccacaata
     9781 agcttaggct tatgctctaa cgctaattta cgaacttcat cataattgat tcgttctgtg
     9841 tctttatcta ctccatattc aacgaaattg tagaatttac cactaaaatt aacaggcgct
     9901 ccatgtgtca agtgaccacc atgactcaaa ttcataccta aaactgtgtc gcccattcct
     9961 aatgcaacta agtaaacagc catgttcgct tgtgaacctg aatgtggttg aacattgaca
    10021 tgttcagctc caaacaatgc tttagcacga tcaattgcga tgctttcagt aacatctaca
    10081 aactcacagc caccataata tcggcgtcct ggatagcctt cagcatactt attagtcaac
    10141 actgaacctt gtgcttccat aaccgcttcc gatacaaaat tttccgatgc gattaactct
    10201 atgttgctat tttgtctctg aaattctctc tcgattgctt ctgcgataac tttatcttgc
    10261 ttggtgatat aagacataaa atctcccctt ctttcaaaaa aacttattgg tatttagcac
    10321 gttcgccacc aatctttttc ggcctagatg tggcaatagt tacaattgcc tgtcctactt
    10381 gctttactga ggtccttaca ggtacacata catgcttaat atgcatgcct attaacgttt
    10441 gaccaatatc aattccacaa ggaacagtaa tatgttcgac cacgatcgga tccttcatat
    10501 gctgaaaagc gtatgttgcc aaactccctc cagcatgtac atctggaacg acggaaactt
    10561 cttccattgt taatggatta tactgagatt tttctattgt tatcgctctg ttgatatgtt
    10621 cacatccttg aaaagcaaaa gtaacgcctg tctctttact cacaacatct aatgcattaa
    10681 aaatagtttc tgcaacttcc atcgaaccga cagtccctat tttttcgcca atgacttccg
    10741 atgttgaaca tccaattaaa catatatctc ctttattaaa aaaggacata tcttttaatt
    10801 cgtctaataa cattgtcaaa tctttcataa aagcccaccc ttcctaaaaa taaaaaagga
    10861 atatagcaaa gtgctacact cctctattat aacttattta actgttaaca tatactaatt
    10921 atacagaatt cctactagca aataatatct tttaatttta aaattaaact tacaagttct
    10981 tcataggtat gtacatacat ttcttttgtt ccaccgtatg gatctataac ttctcctgct
    11041 tcttttacat attcatgcaa tgtgaaaaca tgattttgca aaccaaagtg tgcctctatt
    11101 aattctttgt gcgaatacga catcgtcaaa ataatatctg ctttcaaatc tgcttcagta
    11161 aattgttgcg ataaggtcgt ttcagctaaa tgatgttctt caactaagtc ttcaacataa
    11221 ttcgaaacac cttgattgtt cacagcgaat atacctcttg attcaaattg atgatttggc
    11281 ataacctctt ttgcaatact ttccgctaat gggctacgac atgtgttacc tgtacaaacg
    11341 aataaaatct tcatagttca catcctttaa taatgtgatt acctgcagct tttaacatgc
    11401 gattcataat tgcttctgta ttatcattca gctcaaagcc gtatatatac gccgctgaaa
    11461 tattttcatt ttcatcaagt gaatgtaaca catcataaag attatgactt gcttgtttaa
    11521 catcattgtc atcctgacat aattgaatga attgcgcttc acttggtata aacgccacct
    11581 tattactcgg cacaataaaa gctatagaag accaatcttt accgtcattt ccaattttgc
    11641 tctcaatatc tgtaataatt gtaagtggtg tattgggtga gtaatgctta tacttcatac
    11701 ctggtgcaat tggctgttca gtatcattat aatcagcatg ggcgatacta ttcggaagta
    11761 tttctgtaat cattgctgct gttatagaac caggtcttgc aattttataa ggaaaagatg
    11821 tgcaatctaa aaccgtactt tctaatcctt cttcactttg ttcagcttga acaataccat
    11881 cgatacggcc attcaaatct tgatatacat gattgaaagt tgttggtgaa ggtctaccac
    11941 ttaaattagc acttggagca gctagaggtt catttatgat ttgtaataat tgtctaccta
    12001 cagaatggct tggcattcta acagcaactg atgataaacc tccagaaact tttcgacata
    12061 gatagcctag ctttaacggc aatataaacg aaatagggcc cggccagaat gcctgcatta
    12121 acttttctac gcgtggatcc aaagtatatg taaaatcttt taattgacct ttactgtgta
    12181 tatgaacaat aagcggattg tcagatggac ggcctttagc ttcatatatt ttagctacag
    12241 cttcttcatc tgtcgcattt gctgcaagtc cataaactgt ttcagttggt aaacctatta
    12301 aaccaccgtt taaaacaatg tcttttattt cattaatttt aggatattgc tgtaaatctt
    12361 cattatattc tctaacatcc caaattttag tatccaactt aatcacgcct ttcttattta
    12421 tcataatata aagcaaaaag ctatgcactt aactaatcat agcaaaggca taacttctaa
    12481 ttaccattta aatgagacga ttcgatcgtg gccatttata tctttaataa tgtcgatttt
    12541 tttgtcagga aatttattta aaattattga tttaagtgcc tcaccttgat tgtaaccaat
    12601 ttcaaaaaca actgggctgc ctttttccat aacgtgaggt aaatcttcaa tgattgattc
    12661 ataaatagca tatccatggt tatctgcaaa caatgcctga tgtggttcga atctcgtaac
    12721 cgttggagac atcgtaacca tatctttttc atctatatat ggtggattag atatcaagcc
    12781 gttcaacttg ataccttcat taattaaggg ctttaatgca tcccctgtta aaaattgtat
    12841 ttgtgattga tgcttctcag cattattacg agccatattc attgcttcaa gtgaaatatc
    12901 agtagcaata acatttaaat ccggcttttc acatttcaaa gtaattgcaa gtacaccact
    12961 acccgttccg atatctacga ttgttgcatc atcttctaac tgttgtaaga aatgcaacat
    13021 tacttcttca gtttcaggtc ttggtatcaa acaatttgag tttacatcaa acgttctacc
    13081 ataaaatgag gcaaagccaa ctatatactg tataggctct cctaataaca tacgttgtaa
    13141 tgctaagtcg aacttcataa tcatcgcttt cggcatatca tcatgcatgt ggactacaaa
    13201 gtccgtacgc gtccattgaa atacatctaa cattaaccat tcagctcgtg tttgttcaaa
    13261 ccctttttgt tgtgttaaat gaattgcttc atctaacttt tctttataat tcaccattat
    13321 taagttcttt caatttatct gtctgctctg ataaagtcag tgcatctata atttcttcta
    13381 aatggccttc cataatttgc cctaattttt gaagcgttag acctatacga tggtctgtta
    13441 cacggctttg tggataatta taagttcgaa tacgttctga acgatcacca gtaccgactg
    13501 ctgatttacg ttgtgacgca tacttttgtt gttcttcttg aactttcata tcgtataaac
    13561 gtgcttttaa cactttcatt gctttttcac ggttttgaat ttgagacttc tcagaagatg
    13621 ttgcaatgac accagttggt aaatgggtaa tacgtactgc agagtcagtt gtgtttacgt
    13681 gctgaccacc tgcaccactt gaacgatacg tgtcgatttt taaatcttca tttctaattt
    13741 caatttctac atcttcaact tctggtaaaa ctgccactgt agctgttgaa gtatgaatac
    13801 gtccacctga ttctgtttca ggcacacgtt gaacgcggtg cgcaccattt tcaaatttca
    13861 atttactata cgcgccatta ccagaaactg agaaactaat ttctttgtaa ccaccatggt
    13921 cactttcaga cgcttctact atttcagttt tgaatccttg tgattcagca tactttgaat
    13981 acatacgcat taaatcacca gcaaaaatcg cagcctcatc accacctgct gctgctctta
    14041 tttctacaat aacgtctttg tcatcattag gatctttagg aatcaataat attttaagct
    14101 cttcttcaag atttggaagt tcagctttaa taccattact ctcctctttt aacatttcta
    14161 cttcttcttt atcatcagtc tcacttaaca tttcttcaat atcagctaat tcttcttttt
    14221 tagctttata gttacgataa acatctacag ttttttgtaa atcagcttgc tctttagaat
    14281 atttacgtaa tttatctgaa tcatttacaa catctgggtc acttaacagt tcatttaact
    14341 gttcgtatct ttcttctaca atatctaatt gatcaaacac ttataattcc tccttattat
    14401 tatcactagg tgctacgata tggtgcgcgc gacaacgtgg ctcataactt tcattggcac
    14461 ctactaagat aatcggatca tcgattttag ctggtttacc atttattaat cgttgcgttc
    14521 tactagatga agaaccacaa acagcacaaa ctgcttgaag tttcgttact tgttcactga
    14581 cagccatcaa tttaggcatt ggttcgaacg gttcgcccct aaaatccata tctaatccag
    14641 caacaataac acggtgtcca tctgctgata gtttttctac tatacttaca atttcatcgt
    14701 caaaaaattg cacttcgtct attcctataa catcaacatt agttaagtcg tgcgtcataa
    14761 tttcacttgc tttagaaata ttaatcgctt caatggcatt accattatga gagaccactt
    14821 tttctttatg atatcgatca tcaatcgccg gtttaaatac aacgactttt tgtttagcgt
    14881 atatacccct tcttagacgt cttattagtt cttcggattt accgctaaac atactacctg
    14941 taatacattc tatccaaccg gaatggtaag tttcatacat tgagagttcc acctttttca
    15001 aaacataatc gctttattat atcatatttc aaatattcat aaatgtcttt ttcataatta
    15061 tatcgatatt gtacatgaac aattatttta taaatttgtc ttgcatattt atatacagct
    15121 attctaattt gttgcttctt attcgtgcga tgtttctact caatacaaac tttaataaag
    15181 ggggaaattt atcattatgt attaacaaca tggtattgag tcacttcttt gaacacagtc
    15241 gtaagtatat acccttacta cgcttataaa taaaaaagct gtctacaatt ccattatagg
    15301 atgcagacag cttaacgatt actatattag ttgtttgatt taagaccaaa ctttttgttg
    15361 aaacgttcca cacgtccatc cgcagcagcg aatttttgac gtccagtgta gaatggatgt
    15421 gaatcagatg aaatatctaa acgaataact gggtattctt ttccatcttc ccactccatc
    15481 atttctgaag atgtttttgt agaaccgctt aagaatttga agtttgtagt agtatctaaa
    15541 aagattactt ggtgatactc aggatgaatt ccttgtttca ttattttcag ctcctttgcc
    15601 ctgaaccatc tggaacagag ttatttgtga gtttttaccc aatactgtgt aattataaag
    15661 gtaattaatt caaaacgcaa gcccctatat aatgtttatt aaattatagg tcgacccgtt
    15721 ttagtacttt cttctgcaga cttttgtagc tgcttgaaga aatcttcatt attcttagac
    15781 cttttaagtt tgcgaataaa tctttcagta aagtcagttg agtcagtgaa tagatttctt
    15841 aattgccata atgtgtctaa ttcagattta cttatcaaca attcttcttt acgcgttgaa
    15901 cttctgccaa tatcaattgc agggaagata cgacgttcag acaatttacg atctaaatgt
    15961 aactccatgt tacctgttcc tttaaattct tcgtaaatca tatcgtccat acgtgaaccc
    16021 gtatcaacta atgcagttgc aagtattgtt aaacttccac ccgcttcaat atttctcgct
    16081 gcaccgaaga atgcttttgg tttgtgtaaa gatgcaggat ctaaaccacc tgataatgta
    16141 cgaccacttg gtggaataac taagttataa gcgcgtgcta atctcgttat agaatccatt
    16201 aaaataatga catcttcccc aatttctact aaacgctttg cacgttcaag taataattca
    16261 gctactttaa catggtgttc tggtggttcg tcaaacgttg aatgaacgac ttcagcagct
    16321 tctactgagc gttctaaatc tgttacctct tcaggacgct cgccaactaa caaaataaat
    16381 agctttgcat ctggtttgtt cgtactgatt gcattcgcta tttcttttaa taacgatgtt
    16441 ttacctgctt taggtggcgc cactattaaa ccacgttgac ctaaaccaat cggtgttact
    16501 aaatccatga tgcgcgttga ataattttgt gtttctgtct ctaatttaat acgctcatct
    16561 ggataaagtg gtgtcaaagc ttggaaatgc ggacgtttct tcacttcttc tgcgttatgg
    16621 tcattgacaa agtcaacttg taataagcca taatattttt cgttatcttt aggttttcta
    16681 actttcccag ttactttatc cccacgttta atttcaaaac gacgaatttg gctagcagat
    16741 atataaatat ctttttcccc tttagaatag ttcactgttc ttaaaaaacc ataaccatct
    16801 ggttgtatat catctaagat accttccata taatagttac catctttttc catttgtgct
    16861 tccataatag ctagaacaag ttctttttta tttaatttac tatagttcgt caacttaaga
    16921 gttttagctt tttgagtgag ttccttggta gtatagttct tgtacaattc gtggaatgat
    16981 tcatactgag gagatgtacg ttctctttca ggcatatagt ttacacccat ttcattattt
    17041 tttagttagt aagtaataga taggcatgac aatgcataaa taactgtgct agcacaatcc
    17101 cattatgcta atccccaacc ttttaaatac atgaataact atagcaaatt tttattttaa
    17161 ttacaaaatc tatttataaa aattgcaaaa ttaaaacagt acacaaaact taattagtca
    17221 tgtgcactgt tttattgcga tttttaattc aaaaatcaaa tttatttaaa atatccagct
    17281 atagatttca cttctaagaa ctcttcaata ccataatcgc cccattcacg acctaaacca
    17341 gattgtttat agccaccaaa tggtaaatct ggctttctac ctgcttcgtt tatttctact
    17401 gtacctgctt caatagaacg agctacttta tgcaatgttt ctttgtcctt accaataaca
    17461 tatcctgcca aaccatattt tgtatcattt gcaatttgaa tcgcttcatc taaatcgtta
    17521 taagtgataa ctgacattac tggcccaaaa atttcttctt gtgctatcgt catttgatta
    17581 tctacattaa taaaaattgt cggacgtgca aagtatcctt tttcaagtcc ttctggttta
    17641 ccaggaccac cataaaataa ttcagcacct tcttcaatac ctttattaat ataattttgt
    17701 acttgatcaa attgtttttt actaatgata gggcctactt gtgtaccatc ttctcttgga
    17761 ttaccgacac gcacttggct aaattgttct tttaattcag ctaagaatgc atctttaatt
    17821 ttgttaggca ctaaaacacg tgtaccagct gtacatactt gaccagtatt attaacaact
    17881 ttgcctgttg ttgctttagc cgcttcttta atatctacgt catctaggac gatatatggt
    17941 gatttgccac caagctctaa tgataccttt ttaaaatctt tagcggcttt ttccataatt
    18001 ttagaaccag tagggcctga tcctgtaaat gacatcatgc gtactttagg atgttcagat
    18061 aaaggattcc caacaccagc accatcacca ttaacaaggt taaatacacc tttaggaaca
    18121 ccgactttat caaaaatctc agctaaaata acagctgcaa atggtgtttc ttcagatggt
    18181 ttaagtacaa ctggactacc agccgcaaat gctgctgcta attttaatga tgtttggttt
    18241 gtagggaagt tccacggtgt aattaatcca gatacaccga ttgcttcttt aacaactaaa
    18301 tcatctccgc ggcgttcttc aaattcgtag ttatctaatg cgtctctcgc tgcaacaaaa
    18361 tggtttagtc ccatttgata atggacacgc tcagataatg ataaaggagc acctaattca
    18421 tccgtaatag cttgtacaat atcgtctttt ctgttttcat attcttttac aattttatct
    18481 aataacgctt gtctttcttt cacagatgta tgacggaact ctaaataaac atcgtctgcc
    18541 gcctcgacgg ctttatcaac atcagcttta ttacctttag caactttccc gattacttct
    18601 tcggttgctg gatttataac ttctatcgtt tcattactat tactttctac ccattcgcca
    18661 ttaatgtatt gctttgtgta gtctctcatt attctttcac tcctcaagaa tgatttatat
    18721 attatttatc cgtaatgcat acgatataaa cattacattc cattatgata cgctcccttt
    18781 taatatgttc aaaataaaat gctttaaaat tttgctgcat ctattttttt atatttttaa
    18841 ataaaaataa ctattcccac atcatcgcaa ataatgcgaa gacttagaaa tagttataat
    18901 caatgctatg tttaaacgtc atgtaattat ttagcagtac gttgatctgt taaatcgaca
    18961 tatgattgcg cccatgcttc aattggatgt aaagcctctg ctaacgcttt acctttttca
    19021 gttagcacat aaataatttg tactggactc gtagaaatga tttgcttctc aactaactcc
    19081 cattgtgcaa gctctgacaa cttaagactt aaagcacgtg gtgttattgt tttcaaatct
    19141 cttttcatat cggaaaagtg tgctgaacag tcattacatc ttgagagata attaatgatt
    19201 aatccattcc aacttctacc aagtatttta aaagtttctt cgagatacgg acatacttcc
    19261 atcatcttca cctctcatca ataaatttag aatgtaaacg aacaccattt tttaatcagt
    19321 ataattatta taccacaaaa caagataaat ctatacagtt tccgtccaaa tatctgcacc
    19381 taaagctttt aagtgttcta caatatctgt ataacctcta taaatatgtt taacattgta
    19441 aattgtagtt acaccctcag caattaaacc agcaataatt aaacaagctc ctgctcttaa
    19501 atcgctagca taaacttcag caccatgtaa tgttgatggt tttatcgttg ctgtgccttc
    19561 gtcaacttca atatttgcac ccatgcgctt taattcttca acatgtttaa aacgctccgg
    19621 ataaatcgta tcagttacaa atgaaggacc atttgccata aataataatg gtgtaatagg
    19681 ctgttgcaaa tcagtagcaa aacctggata tactagtgtt ttaatatcaa caaattgata
    19741 tggcgcatta ttattgatgc gaattctttc gtctcttaca tcaacattca cacctaattc
    19801 actaaattta gcagttaatg tttctacatg tttcggaaca atattattta atataacatt
    19861 ttctccacat gctgcagcga tacacatata tgtgcctgct tcaattctat caggtataac
    19921 ttgatactca gaaccatgta attctttgac gccattgatt ttaattgttg atgtacccgc
    19981 tcccttaata ttagctccca tacttgttaa gaagttagca acatcaacta cttccggttc
    20041 tttagcagca ttttcaatta cagtttgtcc tgttgcataa actgcagcta gcataatgtt
    20101 aattgttgca cctacgctaa ccatatctag aaaaatatgt gcacctttta actctttagc
    20161 ttcgattttc attgatgtcg tacttgattc atcaatttca gcacctaaag ctttaaatcc
    20221 tttaatatgt tgatcaattg gacgtggccc aagtggacat cctcccggta aaccaatcac
    20281 acatttttta aatctaccta acatggcacc catcatatag tatgaagcac gtaatgattc
    20341 aactttatta tttggtaatg cagcattttg tatttcagtt gtatcaactt ctaattccgt
    20401 accatttagt gatgccttaa tatttaaatc ttctaataaa ctcactaaag ttttaacatc
    20461 agagatttgc ggtaaccctt ctaatttcac atgtccttga gctaataatg ttgcaggaat
    20521 gatagctaca gcactgtttt tcgcgccact gatattaact tccccattta gtgtgcgtcc
    20581 acctcttatt tttattacct cttgagccat atcatttatc tcctttgtcc attacttttt
    20641 aattttttgc attattagca ttataaatta attttacttt atttgtaaaa tagtttattt
    20701 taatcgttaa aatcttgtct ttctattttc gattgattaa taacaaatca ttcaccttta
    20761 ctaatatatg ctgttcttcc ccacttgacc atggtctaaa gtctgattat tcaactaaat
    20821 attgacgttg ttttgacaat tatttagttt taattctacg gttttcacgt aaactttttt
    20881 cataaattgc tcattttcct tataattata tcacaatcaa ttttgatata ttttaccatt
    20941 tttacgcttt cattttaaaa tcaaactgtt ttatttatta cttatacgac ataaaaaaga
    21001 agcaaacacc attttgtctg cttcttttaa aattaatatc cctacgttat taataactta
    21061 aagactaaat attaattatt tagcgcggtt agaagtaccg aactctttaa ttttaccttt
    21121 aactgtttct ttgatggctt cacgtgcagg tcctaagtat ttacgaggat cgtaaacttc
    21181 tttgtcgtta tttaaaacgt cacgaactgc ttttgctgaa gcgatttggt tttcagtgtt
    21241 tacgttaatt ttagctgtac caaatggaat tgctttttgg atatctttag tcgggatacc
    21301 agtaccaccg tgtaatacta atggtaaacc tgtagataaa ccgatttctt ccatttcttt
    21361 aaatcctaat tttggttcac ctttgtatgg accatgaact gaacctaatg ctggcgctaa
    21421 tgcatcaata ccagtttttt caactagttc ttgacattct ttaggatcag cataaatgat
    21481 gccgtctgct acaacatcat cttcttgtcc accaacagta cctaattcag cttctacaga
    21541 aacacctttt tcatgagcgt attcaacaac ttttttagtt gttgctacgt tttcttcgaa
    21601 tgggctgtgt gaagcatcga tcattactga tgtgaaacca gcatcgatag cttctttaca
    21661 tttttcaaag cttgaaccat ggtctaaatg gattgctaca ggaatagtga tgtttaagtc
    21721 atgcattaac ccttcaacca ttttaacaat tgtgtagaaa ccgctcatgt aacgagcagc
    21781 accttcagaa acacctaaaa ttacaggtgc attttcttct tgtgacgctt ctaaaattgc
    21841 ttgagtgaat tctaggttat taatattgta ttgacctacc gcataaccat tttcttttgc
    21901 atcaattaac atttctttca ttgaaactaa aggcatgaaa gttcctcctt gagtgcttac
    21961 gcacgtattt ttcaaagtaa ttatatcaaa aaaatagcgt caatgcattg aaatactgca
    22021 gattaaatgt gtattttttg aaaaatgcga tagttatact aaatgcaaac gtatacattt
    22081 ttatacaatt taagccgttg cataatatgt agtcattaat tacaatattt tataagtaga
    22141 ttggaggata aagatgtcaa aaatcttaaa cacacaacta ataggtatct ttaataggct
    22201 agaaaaacaa tctttagaaa ttcaaatggc cgcccaatgt ttaattcaag caattggtgg
    22261 cgaaggttat gtttatgtaa aaggttatga tgatttacag tttttcgaaa gtttcattct
    22321 acatagtgat gagcgtttaa aatctagccg aaaacttgac gctattaaag actttaaaga
    22381 aattgactca actgatcgtg tattattatt cgcaccattt tataatgatc aagttgcatt
    22441 agatattcaa aaacttatcg acttagatat cgatgtggtc ttaataagca acaaacctaa
    22501 aacagatgat ttccctgatc atctcgtaca ttttatcgat ttatctacac caagacctat
    22561 agtctacact gaagattacg ataagattgt ccaaccacat gcgatggcat tgaattatgt
    22621 atactatgac atttatacac aaatgattga aatgacaaga gacttagaat tataaaagtc
    22681 actattgtta tttcatacct tgtcactaac ataatattaa aattatacga aaaaccggta
    22741 ctttcaatgt ttaacaatga aagtaccggt tttattagca aatttattta ttttgttgat
    22801 attttaatga agcttcaata aatgatttaa aaatcgggtg cggacgattt ggtctagata
    22861 agaattctgg gtggaattga caagcaataa agaaatcatt tgtcggaatc tctaccattt
    22921 ctactaaacg tccatctgga cttgtaccag aaatcaccat accatttgct tctaattgtt
    22981 ctctatagtc attattaaat tcataacgat gacgatgtct ttcttcaatt tccgctttac
    23041 cataaacatc ttgtgccaat gtgccttctt taattgaaca tggatataag cctaagcgta
    23101 atgtaccacc taaatcttcg atatcttttt gttctggtaa taaatctata attgggtatg
    23161 gtgttgctgg gtctaattca gctgaatgtg cgccttcaag gcctaatacg tttcttgaaa
    23221 attcaactgt tgcaagttgc attcctaaac aaataccaaa gaatggtaca ttgttttctc
    23281 tagcatactt aattgcacta attttacctt cacttgcacg gaaaccaaat ccacctggta
    23341 ctaaaatacc gtcgacatct gcaaggtatt cggctgcatt ttcatctgtt acttcacttg
    23401 aatcaatcca tctaatgtca atatctttgg caaaaggata tccagcatgt ttcaatgatt
    23461 caacaactga taaatatgca tcttgtaagc taacatattt acctactaaa ccaattgtaa
    23521 ttttaccatc taaattatta acgatatcta acaactgttt ccattcatca agctgtgttt
    23581 catatttcgc gtttaattgt aaacgtttaa taacgatatc atccatattt tgttggctta
    23641 attgtaatgg aatttcgtat aaagagtctg catcacgaca ttcaataaca ctttctttat
    23701 taatgtcaca gaataatgca attttatctt ttaaatcttg tgtcatttca tattcagttc
    23761 ttacaacgat taagtctggt tgaataccta agcctcgtaa ttctttaaca ctatgttgtg
    23821 ttggcttcgt tttcatttct ccagcagctt taatataagg cagtaatgta cagtgaacat
    23881 acataacatt ttctctacct aaatcgctac gaatttgacg aatcgcttca ataaacggta
    23941 atgactcaat atcacctgtt gtaccgccaa tttcagtgat aacaacgtct gcattcgtac
    24001 tttcccctgc aagtaataaa cgttctttaa tttcatttgt aatatgcgga ataacttgaa
    24061 ctgttccgcc taagtaatca ccacgacgtt cttttttcaa tacgtgtgaa tacactttac
    24121 cggctgtcac atttgaaaac ttgtttaaat taatatcaat aaatctttcg taatgtccta
    24181 agtctaggtc agtttctgca ccatcatccg ttacgaatac ttcaccatgt tgataaggac
    24241 tcattgtacc tgggtcaaca tttaagtatg gatcgaattt ttgaattgtt acatttagac
    24301 ctctatcttt taataatcta cctagagaag atgctgtaat acccttccct aatgatgaaa
    24361 ctacgccacc tgttacaaaa ataaattttg tcatgtcttg acctcctaat tattaaaggg
    24421 aatgcttcct catcaattgt aaaaattacg taaaacataa cattaaagca agctcattca
    24481 agttgtttat ttgtcgaatt cgacaaaatt aaattctgtt tacttaccgt ttccatactg
    24541 ctatgtcact atgtatatca tttacaactt tgatttttgc gttatcattt cttgatttac
    24601 tcaattaaat aaaaaaacgc tccctctcac aaattctgta aggggagcgt ataaatttta
    24661 tacacgtgtc ctatttaaag gagcccgaat aaaattttat catctttcaa ctaaaagtca
    24721 aacaaaaaat taatcgttga agtcttcttc gtcttcaaac acttcttcgt cttcgtctag
    24781 ttcatcttca tcttcttcaa tgactatatc cgaatgattg atttcttctt caacctgctc
    24841 atcttctgga tcatttagtt cttcttgatc atctgtttga gctggaatat catcgtcgtc
    24901 atccatttca tcttcgccca ataattttaa gttttgatct tcttcatcat ctgcatccag
    24961 aatatcgaat ttttgaatag ttggtgcgat tttctcttca atatcatcta ccgaatacca
    25021 atcacgtaat ccccataaat tttctccaac atttaaaaaa cgaccatctg tgtttaaatc
    25081 cgtgtaaaat tgtacaacac gattttcaat ttcttcgtac tcataatcac ctaacgctct
    25141 aaattcatcg atgatatcat ataagttcat tgtttcgcct ttatcattca ataatgtata
    25201 agccatatca ataaatgatt tttcatcaac catttgtttt gtataatctt gaattttcat
    25261 tgcccaatac ttcctttcga aggatataat ctaaatttat acgtctgaat tcatttatat
    25321 atagtaacaa aactaaatta taaatgacaa tatcatattt cgcaatcact gttagtaatt
    25381 taaatatttt tcgaaaacga cataatcatc agtttgttcg atttcaacga aattactatt
    25441 taaacataat ggtttaaaat cattattctc tccaaacaga atcatcgtaa tcacattaag
    25501 gttgtcatag ttttttctta agtaagttgg caattgtcgc agagcacttg aaccaatacc
    25561 attaaagcga tatgatttat ccacttcaat agcagcaatg ttcatgcgtt caccaatctg
    25621 ctccaatgcg ataaagccaa ccttttggcc atcaatcatt aaattaatga tatgtacatt
    25681 ggtcacttct gaacgacttt cttcttcaag ctttaatgat gaaacatgtt ttgaatctaa
    25741 gtccaagtaa tatagtcgtt ctttgccaat tggtccttct tctttagtcg ctgtatgcat
    25801 gaatccagca cgttcgtata ggttccaagc attgtcattt tccgcatcta ctactagata
    25861 tagatgatta aaatcaggaa atacaccttg aacatattgc ggcaatgaca tcattatttt
    25921 cgtgccatat ccaaaacctt gatatttttc attaatcgat aatgaacgaa tataaacgac
    25981 attttcaggt gtatcataac cttcatgctg atagtaacga tgtaatacaa aaaagcccac
    26041 cacttcattt ttatcgttta ttgctacgtt agcaatccta tcagcatcat taattgcatc
    26101 atcaataact tctttaggta aagatgaata tatttgttgt ctttcattca agtcaaaatc
    26161 attaattgct gaacgatatt gttcatcaaa tgcttttaaa gtgatacctt caaaacaaag
    26221 ttctttagcc atatatttaa cccctatctt tttatatatg aagcatgaat ccccatatta
    26281 ttttacctat tatttcacta cgataccat
//
LOCUS       DALAAR010000036        25841 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000036 DALAAR010000000
VERSION     DALAAR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.037"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2458_001517"
     CDS             complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2458_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCZ3887383.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1061..1543
                     /gene="purE"
                     /locus_tag="O2458_001518"
     CDS             1061..1543
                     /gene="purE"
                     /locus_tag="O2458_001518"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCZ3887384.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1530..2654
                     /gene="purK"
                     /locus_tag="O2458_001519"
     CDS             1530..2654
                     /gene="purK"
                     /locus_tag="O2458_001519"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCZ3887385.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2658..3362
                     /locus_tag="O2458_001520"
     CDS             2658..3362
                     /locus_tag="O2458_001520"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCZ3887386.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3362..3625
                     /gene="purS"
                     /locus_tag="O2458_001521"
     CDS             3362..3625
                     /gene="purS"
                     /locus_tag="O2458_001521"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCZ3887387.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            3627..4298
                     /gene="purQ"
                     /locus_tag="O2458_001522"
     CDS             3627..4298
                     /gene="purQ"
                     /locus_tag="O2458_001522"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCZ3887388.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4291..6480
                     /gene="purL"
                     /locus_tag="O2458_001523"
     CDS             4291..6480
                     /gene="purL"
                     /locus_tag="O2458_001523"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCZ3887389.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6459..7943
                     /locus_tag="O2458_001524"
     CDS             6459..7943
                     /locus_tag="O2458_001524"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCZ3887390.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            7936..8964
                     /locus_tag="O2458_001525"
     CDS             7936..8964
                     /locus_tag="O2458_001525"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCZ3887391.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            8967..9533
                     /gene="purN"
                     /locus_tag="O2458_001526"
     CDS             8967..9533
                     /gene="purN"
                     /locus_tag="O2458_001526"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCZ3887392.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9548..11026
                     /gene="purH"
                     /locus_tag="O2458_001527"
     CDS             9548..11026
                     /gene="purH"
                     /locus_tag="O2458_001527"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCZ3887393.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11048..12295
                     /locus_tag="O2458_001528"
     CDS             11048..12295
                     /locus_tag="O2458_001528"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCZ3887394.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(12560..13366)
                     /locus_tag="O2458_001529"
     CDS             complement(12560..13366)
                     /locus_tag="O2458_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ3887395.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13359..14759)
                     /locus_tag="O2458_001530"
     CDS             complement(13359..14759)
                     /locus_tag="O2458_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887396.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(14774..15349)
                     /locus_tag="O2458_001531"
     CDS             complement(14774..15349)
                     /locus_tag="O2458_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ3887397.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15426..15526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2458_001532"
     CDS             complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2458_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCZ3887398.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16219..17514
                     /locus_tag="O2458_001533"
     CDS             16219..17514
                     /locus_tag="O2458_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887399.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            17939..19111
                     /locus_tag="O2458_001534"
     CDS             17939..19111
                     /locus_tag="O2458_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCZ3887400.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19165..19707
                     /locus_tag="O2458_001535"
     CDS             19165..19707
                     /locus_tag="O2458_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCZ3887401.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            19861..20127
                     /locus_tag="O2458_001536"
     CDS             19861..20127
                     /locus_tag="O2458_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCZ3887402.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20130..21848
                     /gene="ptsP"
                     /locus_tag="O2458_001537"
     CDS             20130..21848
                     /gene="ptsP"
                     /locus_tag="O2458_001537"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCZ3887403.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="O2458_001538"
     CDS             complement(22085..22318)
                     /gene="nrdH"
                     /locus_tag="O2458_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCZ3887404.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            22516..23877
                     /locus_tag="O2458_001539"
     CDS             22516..23877
                     /locus_tag="O2458_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCZ3887405.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            23874..24893
                     /locus_tag="O2458_001540"
     CDS             23874..24893
                     /locus_tag="O2458_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCZ3887406.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            25026..25688
                     /locus_tag="O2458_001541"
     CDS             25026..25688
                     /locus_tag="O2458_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCZ3887407.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         8941 a         3700 c         4859 g         8341 t
ORIGIN      
        1 tacgaatcaa tacctcgacg cattttttct gcaagcaaag tattatttaa taccatagta
       61 attgttaatg gaccaacgcc accaggaact ggtgtaatag ctccagcaat ttctttaacc
      121 gcatcataat caacgtcacc ttttaatttg ccattttcat ctggcgtatt gccaacatcg
      181 ataattactg ctccttcttt gaccacatct tttgttacta aaccaggctt accaactgca
      241 ctgacaatga catcagcatc ttttaaatat gatgccatat cttttgaacg agaatgtaag
      301 attgttactg atgcattttt ttgaagtagt aacttagaaa ctggttgtcc gacaatatga
      361 cttcgtccaa ttacaactgc atttttacct tctaaatcaa tatcagcatg ttttaatatt
      421 tccatgatgc cgagcggtgt gcaaggtaca aaagtttgtt catcgatata taatttccct
      481 atatttattg gatgaaaacc gtccacatct ttttcaggat tgattgcttc taatattttc
      541 tgttcgctaa cttgttttgg taatggtact tgtaccaaaa taccacttac agaatcatca
      601 ttatttagtc tatttagttc gtttaatact tcttcttcag tagctgtttc ttccaaatgt
      661 acgatttctg aaatcatacc aattttttca gctgctttct tttttgatct aacataactt
      721 tgactagcgc catcattacc aactaatata acggataatt taggtgtaaa acccttttct
      781 tttagcgctt caacttgatc ttgtaacccc tgtctgtagt ctttggcaat ttgtttacca
      841 tctaaaattt tagcaaccat aaaaacttct cctcctattg tcaatatcct aactttgtta
      901 gtttataaaa ctaatttaca tagcaaaaga cgtaaattca aacaaaaaat caaaataaag
      961 ttcgattttt gattgaaaaa gcagaaattg cttgttatgc tatatctata atatacaact
     1021 aaagtaattc acaattcaaa cttttgaaag ggtgtacaaa gtgaaagtag cagtcattat
     1081 gggcagttct tccgattgga aaattatgca agagagttgt aacatgttgg attattttga
     1141 aattccgtac gaaaaacaag tagtatccgc acatcgtacg ccaaaaatga tggttcaatt
     1201 tgcttctgaa gcgagagaaa gaggtataaa cattatcatt gcaggcgctg gcggtgcggc
     1261 acatttacca ggtatggttg catcattaac gacgctacca gttattggag tgccgattga
     1321 aacaaaaagt ttaaagggta tagattcttt attatcaatt gttcaaatgc caggaggtat
     1381 tccggttgca acgactgcaa ttggtgcagc aggtgctaaa aacgcaggta tacttgcagc
     1441 aagaatgtta agtattcaaa atccttcttt agttgaaaaa ctaaatcagt atgaatcttc
     1501 gttaattcaa aaagtggagg acatgcaaaa tgaacttcaa taaattaaag tttggtgcga
     1561 ctattggcat tattggtggt ggtcagcttg gaaagatgat ggcacaatca gctcaaaaaa
     1621 tgggttataa agtggttgta ttggatcctt ctgaagattg tccatgtaga tacgttgcac
     1681 acgaatttat acaagccaag tatgacgatg aaaaggcact caatcaatta ggacaaaaat
     1741 gtgatgtgat tacttatgaa tttgaaaaca tttcagccca acaattaaaa ctattatgtg
     1801 aaaagtacaa tattccgcaa ggttaccaag ctatacagtt attacaagat cgcttaactg
     1861 aaaaagaaac attaaaaagt gctggtacca aagttgtccc gttcatttca gtaaaagaat
     1921 ctacagatat tgacaaagca attgaaacat taggatatcc ttttattgta aaaactagat
     1981 ttggtggcta cgatggcaaa ggtcaagttt taattaacaa cgaaaaagac ttacaagaag
     2041 gttttaaatt aattgaaact agtgaatgcg tagctgaaaa atatttgaat atcaagaaag
     2101 aagtatctct tactgttaca agaggaaaca acaatcaaat cacttttttc ccattacaag
     2161 aaaatgagca tagaaatcaa atacttttca aaacaattgt tccagcgaga atagataaaa
     2221 cagctgaggc gaaagagcaa gttaataaaa ttatccaatc gattcatttc attggaacat
     2281 ttacagttga attttttata gatagtaaca accaattgta tgtgaacgag atagcaccaa
     2341 ggcctcacaa ttccggacat tattcaattg aagcatgtga ttattcacaa tttgatactc
     2401 atattttagc agttaccgga caatcattac caaattcaat tgaattatta aagccagcag
     2461 tcatgatgaa cttactaggt aaagatttag atttattgga aaatgaattt aatgaacatc
     2521 cagagtggca cttacatatt tatggtaagt ctgagcgtaa agatagcaga aaaatggggc
     2581 atatgactgt actaacgaat gatgtaaacc aaactgaaca agatatgtac gctaaatttg
     2641 aggggagtaa ttaaagcatg acattattat atgaaggaaa agcgaagcgc attttctcaa
     2701 caaatcaaga aaatgaatta agagttgaat ataaagatga agttactgct ggaaacgggg
     2761 ctaagaaaga cacaatggca ggtaaggggc gattaaataa tcaaattact tctattatat
     2821 ttaaatattt acaagaaaat ggaatagaaa gtcactttat taaacaatta tctgaaacag
     2881 aacaattagt taagcctgtg aaaataattc cattagaagt agttgttcgt aatattgcta
     2941 gtggatctat tacaaagcgt ttaggttttg aaaatggtga agtttttaga gaaccacttg
     3001 tagaattttt ctataaaaat gatgcgttaa atgatccgtt gataacggat gaccatgtta
     3061 aattgctcaa tatagcatca gatgaagata ttgaaatact aaaatccaaa gcattaaaga
     3121 ttaataatgt gttgaaacaa ttaatggatg ctatgaattt aaaattagta gattttaaaa
     3181 tcgaatttgg aaagactgag actggtcaaa ttttgttagc ggatgaaata tctccagata
     3241 catgtcgaat ttgggataaa gctaccaatg caaactttga taaagatgta tatagaaata
     3301 acactggatc actgattgaa acatatcaaa tatttttaaa caaattggag gatttaaaat
     3361 aatgaaaaca attgaactac atatcacatt acaaccacaa gtattagata cgcaaggaca
     3421 aacgcttact cgagctgtac atgacttagg ttatgcacaa gtgaatgata ttcgtgtagg
     3481 aaaagtatta tatatgacag tggatgaggt tagtgatgaa aaggtacaca acattattac
     3541 aactctaagt gaaaaattgt ttgcaaatac agtgattgaa gaatatagct ataaagtgtt
     3601 agatgatgaa aaggagaatg cataaaatga aatttgcggt tcttgttttt ccaggttcga
     3661 attgtgatag agacatgttt aatgctgcta ttaaaagtgg tgttgaagcg gaatatgtag
     3721 attatagaga aacatcacta agtggatttg atggcgtact tattcctggt ggattttcat
     3781 tcggggatta cttaagatct ggggcaatgg ctagtgtagc gccgattatt tcggaagtta
     3841 aacgtcttgc agctgaaggt aagccagtat taggtgtttg taatgggttt caaattttaa
     3901 ctgaaatagg cttattacct ggtgcattat tgcataacga ttcacattta tttattagta
     3961 gaaatgaaga gttagaaata gtgaataatc aaacggcatt tacaaatctt tatgaacaag
     4021 gtgaaaaagt tatatatcct gtagctcacg gtgaaggtca ttattattgt actgatgaaa
     4081 tatatcaaca attaaaagct aacaatcaaa ttattctgaa atatgtgaat aatccgaacg
     4141 gttcatatga tgatattgca ggaattgtta acgaaaaagg caatgtatgt ggcatgatgc
     4201 cacatcctga aagagcttta gaaacgttgt taggtactga tagtggtgtg aaattatttg
     4261 aagcgatggt aaaaagttgg agggaacaac atgtctaaat ttatcgaacc aagcgttgaa
     4321 gaaattaaac ttgaaaaagt atatcaagat atgggattaa gtgatcaaga atatgaaaaa
     4381 gtttgcgata ttttaggcag acaacctaac tttacagaaa caggtatctt ttctgttatg
     4441 tggagtgaac attgctctta taaacattct aaaccgtttt taaagcaatt tcctacgtca
     4501 ggtgaccatg tgcttatggg gcctggtgaa ggtgcagggg tagtcgatat aggtgataat
     4561 caagccgtag tatttaaagt agagtctcac aatcatccat cagcaattga accatatcaa
     4621 ggggctgcta caggcgttgg tggaatcatt cgtgacattg tctctattgg ggctagacct
     4681 attaatttgt taaacagtct tagatttgga gaattagata ataaacaaaa ccaaagatta
     4741 cttaaaggtg ttgtaaaggg tatcggaggt tatggtaact gcattggtat tccaacaact
     4801 gctggtgaaa tcgaatttga tgaacgttat gatggcaatc cacttgttaa tgcaatgtgt
     4861 gttggtgtta tcaatcacga catgattcaa aaaggcacag caaaaggtgt aggtaattcg
     4921 gtcatttatg ttggtttgaa aactggtcga gatggtattc atggtgctac ttttgcatct
     4981 gaagaattga cggaagaaag cgaaagtaaa cgaccttctg tacaaatcgg tgatccattt
     5041 gtaggtaaaa aattaatgga agcaacactt gaagcaatta catttgatga attagttggt
     5101 attcaagata tgggtgctgc tggtttaaca tcttcatcgt ctgaaatggc ggcaaaaggt
     5161 ggtagtgggt tacatttgag attagaacaa gtgccaacac gtgagccagg tatttctcct
     5221 tatgaaatga tgctttcaga aactcaagaa cgtatgttac tagttgttga aaaaggtact
     5281 gaacaaaaat tcttagattt atttgataag cacgaattgg atagtgctgt tataggtgaa
     5341 gttacagata caaatcgttt tgttttaaca tatgatgacg aagtttatgc tgacattcca
     5401 gttgaaccac tagctgatga agcacctgta tatattttag aaggagaaga aaaagattat
     5461 aatacttcta aaaatgatta tacacacatc gatgttaaag atactttctt taaattactt
     5521 aagcatccga ctatagcatc taaacactat ttatatgatc aatacgacca acaagttggt
     5581 gccaatacga taattaagcc aggacttcaa gcatcggtag tacgtgtgga aggcacaaat
     5641 aaggcaattg cttcaacaat tgatggtgaa gcgcgttatg tatataacaa tccatatgaa
     5701 ggtggaaaga tggtagtagc tgaagcttat cgaaatttaa ttgccgtggg tgcaacacca
     5761 ttagcaatga cagattgttt aaattatggt tctcctgaaa agaaagaaat ctatcaacag
     5821 ttgatagatt caacgaaagg tatggcagaa gcatgcgaca ttcttaagac accagtagtt
     5881 tctggtaatg tatctttata taacgaaacg aaaggtactt ctattttccc aacaccagtt
     5941 gttggaatgg taggtttgat tgaaaatgta aattatttaa atgattttga acctcaagtt
     6001 ggagataaat tatatttaat cggtgatact aaggacgact ttggtggtag tcaacttgaa
     6061 aagttaattt atggcaaagt taatcatgaa tttgagtcat tagatttgag ttcagaagtt
     6121 gaaaaaggtg aatcaatcaa gaccgctatt cgtgaaggac tattatcaca tgttcaaaca
     6181 gttggtaaag gtggcttact gattacctta gctaaactaa gtgcgcatta cggtttagga
     6241 ttaaaatctt caatagatat aacaaatgca caattgttta gtgagacgca aggccgatat
     6301 gttgtttctg ttaaatcagg taaaacttta aatattgata atgcaataga aattggactt
     6361 ttaacagata gtgataattt caaggtaaca acaccatata cagagattag tgaaaatgtt
     6421 tcagatatta aacaaatatg ggaaggggca attgctcaat gtttaactac tcaggattaa
     6481 acgaagaatg tggcgtgttt ggtatttgga atcatcctga agcagcgcaa ctaacatata
     6541 tgggacttca tagtttgcaa catcgtggtc aagaaggtgc aggtatagtt gtttctgatc
     6601 aaaatgaatt aaaaggcgag cgaggattag gcttactaac tgaagcgatt aaagatgatc
     6661 aaatggaacg attaaaagga tatcaacatg caattggtca cgtccgttat gctacttcag
     6721 gtaataaagg tattgaaaat attcaaccgt ttctgtatca cttttatgat atgagtgtag
     6781 gtatttgtca taatggtaac ctcattaatg ctaaatcatt gcgtcagaat ttagaaaaac
     6841 aaggtgctat cttccattcg tcttctgata ctgaagtcat tatgcatttg atacgtcgaa
     6901 gtaaagctcc tacttttgag gaagcgttga aagaaagttt gcgaaaagtt aaaggcggtt
     6961 ttacatttgc gattttaact aaagatgcgt tatatggcgc agtagatcca aatgctatca
     7021 gaccacttgt tgtaggtaaa atgaaagatg ggacatacat ccttgcaagt gaaacatgtg
     7081 caatagatgt gttaggtgca gaatttgttc aagatattca tgcaggtgaa tatgtcgtga
     7141 ttaacgataa aggtattaca gttaaatctt atacacatca tacgacaact gcaatttctg
     7201 cgatggaata tatttatttt gctagaccag actcaacaat agctggtaaa aatgtccatg
     7261 cagtacgtaa agcttctggt aaaaaattag cccaagaaag ccctgtaaat gctgatatgg
     7321 tcatcggtgt acccaattca tcgctatcag ctgcgagtgg ttatgctgaa gaaataggtt
     7381 tgccatatga aatgggacta gttaaaaatc aatatgttgc aagaacattt attcaaccaa
     7441 ctcaagaatt acgtgagcaa ggtgtgagag tgaagttatc tgcggtaaaa gatatagtag
     7501 atgggaaaaa tatcattctt gttgatgatt ccattgttcg cggtacgaca attcgacgca
     7561 ttgtgaaaat gttaaaagat tctggtgcaa ataaagtgca tgtgcgtata gcatcaccgg
     7621 aatttatgtt tccaagtttt tacggaatcg atgtttcaac tacggcagaa ttaatttctg
     7681 caagcaaatc acctgaagaa attaaagatt atattggcgc tgattcatta gcatatctat
     7741 ctgtagatgg gttaattgaa tcaattggtt tagattatga cgcgccatat agtggcttat
     7801 gtgtagaaag tttcactgga gattatcctg cagggttata tgattatgaa gcaaattata
     7861 aagcgcattt aagtcatcga caaaagcaat atatttctaa aaacaaacac ttttttgata
     7921 gcgagggaaa tttaaatgtc taaagcatat gaacaatctg gtgtaaatat tcatgctggt
     7981 tatgaagctg tagaaagaat gtctagtcat gttaaacgta cgatgcgtaa agaagttatc
     8041 ggtggtttag gtggattcgg tgctacattt gatttatcac aattaaatat gacagcgcca
     8101 gttttagttt ctggaacaga cggagtaggt acgaaattaa aactagctat cgactatggt
     8161 aaacatgatt cgataggtat cgatgcagtc gcaatgtgtg ttaatgatat tttaacgaca
     8221 ggtgcagaac cattatattt tttagattac attgctacaa acaaagttgt tcctgaagtt
     8281 attgaacaaa ttgttaaagg tattagtgat gcatgtgttg aaacgaatac tgcacttatc
     8341 ggtggagaga ctgctgaaat gggtgaaatg tatcacgaag gcgaatatga tgtagctgga
     8401 tttgctgttg gagcagttga aaaggatgac tatgtagatg gttcagaagt gaaagaggga
     8461 caagttgtta tagggcttgc gtcaagtggc attcattcaa atggatacag tttagtgcgc
     8521 aaattaatta atgaatcagg cattgatttg gcatcaaact tcgataatcg tccatttatc
     8581 gatgtctttt tagaaccaac taaattatat gtcaaacctg tacttgcttt gaaaaaagaa
     8641 gtttctatta aggcaatgaa tcatattact ggtggaggtt tttatgaaaa tattccacgt
     8701 gcattgccag ccggatatgc tgctagaatt gatactacat catttccaac accaaaaata
     8761 tttgattggt tacaacaaca aggcaatata gacacaaatg aaatgtataa catttttaat
     8821 atgggtattg gctatacggt tatcgttgat gaaaaagatg tatcacgcgc tttgaagatt
     8881 ttagcagaac aaaatgtgga agcctatcaa attggtcata ttgtgaaaaa tgagtcaact
     8941 gcaattgaat tgttgggggt ataacaatgg ttaaaattgc gatttttgca tcaggttcag
     9001 gaagtaactt tgaaaatata gttgagcatg ttgaatcagg aaaacttgaa aatattgaag
     9061 ttacggcgct atatacggat catcaaaatg cgttttgtat agatagagca aaaaagcacg
     9121 atattcctgt ttatattaat gaaccaaaac aatttgattc aaaagcagcg tatgaacaac
     9181 atttagtaac attattaaat gaagataagg tagagtggat tattctagct ggctacatgc
     9241 gtctaattgg tccagactta ttagcttcat ttgaaggtaa aatattgaat atacatccat
     9301 ctctattgcc gaaatataag gggattgacg caataggcca agcatatcat agtggcgata
     9361 ctattactgg ttcgacagta cattatgttg atagtggtat ggatacggga gaaattattg
     9421 aacagagaaa atgtgatatt agaccggacg attcaaaaga acaattagaa gagaaagtaa
     9481 aaaaattgga atatgagtta tatccaagtg ttattgctaa aattgtaaaa taaggagtat
     9541 gtatgtgatg aagaaagcta ttttgagcgt atcaaataaa acaggtattg tagagtttgc
     9601 taaagcgtta acgcaattaa attatgaatt atattcaaca ggtggtacta aacgtatatt
     9661 agatgaagca aatgtaccag ttcgttctgt ttcagactta acacattttc cagaaataat
     9721 ggatggccgt gttaaaacat tacatccggc tgttcatggt ggcattttag ctgatcgaaa
     9781 taaaccgcag catttaaatg aattatcaga acaacatata gatttaattg atatggtagt
     9841 agttaattta tatccattcc aacaaactgt tgcaaaccct gatgtgacga tggacgaagc
     9901 aattgaaaat attgatattg gtggtccaac aatgttacgt gctgcagcta aaaactataa
     9961 acatgtaaca acaattgtac atccggcaga ttatcaagaa gtattgacgc gattaagaaa
    10021 cgattcgtta gatgagtcat atagacaatc attaatgatt aaagtttttg agcatactgc
    10081 agaatatgat gaagcgattg tacgtttctt taaaggggat aaagaaactt taagatatgg
    10141 agaaaatcca caacaatcag cgtattttgt gagaacttcg aatgctaagc acacgattgc
    10201 aggcgctaaa caattacatg ggaaacaatt aagctataac aatattaaag atgcagatgc
    10261 tacactagct ttagttaaaa agtttgatac acctgctaca gttgcggtta aacacatgaa
    10321 tccatgtggt gttggtatcg gtgacacgat agaacaagca tttcaacatg cgtatgaagc
    10381 ggatagtcaa tcaatatttg gtggaattgt tgcattaaac cgagctgtaa cacctgagtt
    10441 agcagagcaa ttgcatagta tctttttgga agtcattatt gcaccaaaat ttacagatga
    10501 agcattagat attttaaaac aaaagaagaa tgtaagatta ttagaaattg atatgactat
    10561 agacagtaac gaagaagagt ttgtttcagt atctggcgga tatttagttc aagataaaga
    10621 caattatgtc gtgccaaaag aagaaatgaa agttgttaca gaagtagcac ctacagatga
    10681 acaatgggaa gcaatgttat taggatggaa agttgtacca tcagtaaaaa gtaatgcaat
    10741 tattttaagt aataataaac aaactgtagg tataggtgct ggacaaatga atcgtgtcgg
    10801 tgctgctaaa attgcgttag agagagctat tgaaatcaat gatcatgtag cgttagtatc
    10861 tgatggattt ttccctatgg gagatacagt tgaacttgca gcacaacatg gtataaaggc
    10921 aattatccaa ccgagtggtt cgattaaaga tcaagattca attgatatgg ctaataaaca
    10981 tggtattgca atggtagtca caggcactcg acattttaaa cactaatttt aaaggggcgt
    11041 aacataaatg aatgtattag taattggtgc tggtggacga gaacatgcac ttgcatataa
    11101 acttaatcaa tcgaatctag ttaaacaagt gtttgtcatt ccaggtaatg aggcaatgac
    11161 acctatagct gaagtacaca ctgaaatttc agaatctgat catcaagcga tactagattt
    11221 tgctaaacgg caaaatgttg attgggtagt tataggtcca gaacagccgc taattgatgg
    11281 attagcagac attttacgag cgaatggttt caaagtgttt ggtccaaata agcaagcagc
    11341 tcaaatcgaa ggctcaaaat tatttgctaa aaagataatg gaaaaatata atattccaac
    11401 tgctgattat aaagaagttg agcgaaaaaa ggatgcttta acatatattg aaaactgtga
    11461 attgcccgtt gttgtcaaga aagatgggtt agctgctggg aaaggcgtta ttattgcaga
    11521 tactattgaa gcagccagaa gtgctattga gattatgtat ggtgatgaag aagaaggtac
    11581 tgttgtattt gaaacgtttt tagaaggtga agagttctcg ctaatgacat ttgttaatgg
    11641 tgatttagca gtacctttcg actgtattgc acaagatcat aaacgcgcat ttgatcatga
    11701 tgaaggacca aatactggtg gtatgggggc ttattgtcca gtaccacata ttagtgacga
    11761 tgttttaaaa cttacaaatg aaacaattgc acaacccatt gcaaaggcaa tgcttaatga
    11821 aggttatcaa ttcttcggtg tattatacat tggtgctatt ttaactaaag atggtccaaa
    11881 agtaatagaa tttaatgccc gttttggtga tcctgaagct caagtattat taagtcgcat
    11941 ggaaagtgat ttaatgcagc atattattga tttagatgaa ggaaaacgta ctgaattcaa
    12001 atggaaaaat gaatctattg taggggtcat gttggcatca aaaggatatc ctgatgcata
    12061 tgaaaaaggg cataaagtaa gtggctttga tttaaatgaa aactattttg ttagtggatt
    12121 aaagaagcaa ggtgatacct ttgttacttc aggtggtaga gttatacttg ccatcggaaa
    12181 aggtgacaat gtacaagatg cacagcgaga cgcatacaaa aaagtatcac aaatacaaag
    12241 tgaccattta ttctatcgtc atgacattgc gaataaagca ctacaactta aataagtaaa
    12301 tttaaaatac taagattagc tatgaacgaa tctataacga tagatttttt catagctttt
    12361 ttagttgtag agtctaggac attgatttct gtaccaaatt tgtgattatg catatgtaat
    12421 acaaaagagg cgccacaaca tgtttggatg aacaaaataa catgtttgtg gcacctcttt
    12481 tgtttagtat ggaataaatg gttttctttt tctatacaat gaatttctaa tttagtatct
    12541 atacaattat ggataaaatt taacctacac gaccaagacg aacatcatct atgcccgtga
    12601 tgggtaaggt gattgaacaa taatatgcca tagtaataat ggcaattaaa actataataa
    12661 agattatatc tttatatgag aaaggtacgt tgtaatagta agtacgagga ccatctctaa
    12721 atcctttcga ctccatcgca actgataatt gatgtgcctt tctaatattt tggcttaata
    12781 gaggtataat taaatgctta aatcgcttta accctctata atttgccgcg tctatcatct
    12841 gatagcgcat ttttaaagat ctgcgaagct gtattaaaga actaatcatt aaaggtatca
    12901 tacgaatggc agccatgaat gcataagcaa cttttgattt aacctttaaa tgttgcatta
    12961 aactataaaa tatcatgaca acctgagatg taagtgcgat taagataccg aaaaatgaaa
    13021 tagcaatggt tcttaatgat acatgtaaac cacgaactaa actttctgtt gtaatatgga
    13081 taaatccgaa tttcaaaatt gtatggctac cattcccgta taaaatcatg aacagggaag
    13141 agagtaatgc aaagccaata cttatagtta taaaaattgc tgtaatttta aactgagtac
    13201 cattaaacat caataagaaa actaacatta agatagtgat ataaagcata aaatcgaaat
    13261 tatgcacaaa tataataaag aaaaatagta taattccaag aaatagtttc gttataatgt
    13321 tgacatcatc aacaaatgat tgccgaactt tccattgctc atacattcgt atcaccatca
    13381 caatctagta acgcaccatc tgaaatttta agtcttcttg atggataacg ttcaattatt
    13441 tcatcgtcat gtgtaaccat gacaatactt tgtcccaaat taattcgctt ttggaaaagt
    13501 ttgatcaact ggaatgtatt atggctatca agtccaaatg tcggttcatc taaaaagata
    13561 atatcagctt tagaacttag tgcggtagct acgctaaggc gtcgtttttg accaatagac
    13621 aactcataag gatgttgatc ttttacattt tgtaaatcta aaagttttaa aagttgtatc
    13681 gtttcatcat cactttgatc tttagaaagg tgattaaaat gaatgttaat ttcatcataa
    13741 accgaatttg ttataaattg taattctggg ttttgataaa ctaggtacat gtgttttgct
    13801 gcatgtttaa tttttgttaa acgctgattt tcaaaataaa catcaccttg atatttaatc
    13861 aattgcataa ttgattcaag caaggttgtt ttaccactac catttgcccc tgtaattgta
    13921 atccactcac ctagaccaat ttctaaatct gagaatgaga gcaatgttga tttaccgcga
    13981 ataatacgtc catttttaaa ttgtaataag tgtgagtttg ttgttggaaa gtcaacacga
    14041 cttggtgcga attcccatgc acgtggatgc cacacaccat attcactgag taaatgaaca
    14101 tacttctgta atatgatttc aggacattca tcggcaatga tatttccgtt ataatccatc
    14161 aaaatgacgc ggtcgacatg attccagatg tgtttaactt tatgttcaac gattacaacc
    14221 gtttgatctt cccaaagttc aattagttta gtccataaat cttctgttgc ttgaacatct
    14281 aacattgctg tcggttcatc taaaaacaat gtttttgatt gttgaagaat ggtttcaaca
    14341 attgccaatt tctgtttcat cccgccactt aaatctttga tatacgtttc aggggtaaca
    14401 tttaaattga ccatatttaa agcattgata attaacgcat ccatgtcttc acgtggtaat
    14461 tgtctatttt ctaaaacgaa tgcaagttct tcgtatactt ttggcataca aaactggcta
    14521 tcagggtctt ggaaaataac gccacttaat gggtcaacga ttagttcatc atatttcata
    14581 ggtaattcaa ttaaattagg aacaatacca cttaatacat tcagaagtgt acttttaccg
    14641 caaccagaag gaccgagtaa aagtactttt tctttgtctt gaatagtgat atttaaatga
    14701 tcgaaaattt tacgttgacc acttggatat tttaatcgta aatcacttac ttttaacact
    14761 ctgaatgctc ctcttataag ttgtcgtaat cttctttagc agctggtcta aataattttg
    14821 ttacgcctgt cttatctaaa gcttttacta aaaggtaaga taggacgccg gcgactactg
    14881 caccactaat taatctaaat acgatgaata atgttaagtt ccaacctgca acttcattta
    14941 aataaccata gaaataatct atcgggaaag ccgcgattgc tgtacaaaaa cctgctaaca
    15001 tagctaccat aactgaacgt gattgatatt taaaaattgc aaagacaagt tcacacgcta
    15061 aaccttgtat aaaagcgtaa acgattgtcg gaatatcgaa acgacccata atgatagttt
    15121 cgccggcacc tgcagcaaat tcagccagta aagcaatacc tggttttgga ataattagat
    15181 agcagacaat cgctgccatg aaccaaaccc cgtttgttaa ttgttcgagg tgaaggcctg
    15241 tagcttgcac accattgtaa acaaaccacc ataaattgta aataactgcg aatactactg
    15301 aaataagtac ggttactagt atttcagata gctttaaacc ttttgacatt tttacatcct
    15361 cctaataaaa aaacgcacaa ccatccatag gaaagttatg cgttcacaat atatattagt
    15421 aaaacatatg tatagtaaca ctttcctacg ctagttcaag ctagatcagg ttcaaagggt
    15481 ttgagggcaa gcctcatctc agtataaaac acccctagtg tgtgcgattt atttaattaa
    15541 ttatactgta agacgtttgt aaacttatgt caataggttg tcttcatgaa atttcgttta
    15601 attcgattta aaatttataa tattagcatt ggatttaaat tgaagatgta gtaggaatgt
    15661 tagtaattaa agatataaaa atatgtgaca tgtaataata ttgagctgat aaatgaagag
    15721 ggatacttat caatcatacc tctttaacaa cagtgaagaa cccgtgcata atggcttacg
    15781 aattatagtt tataaggaag aagagggata catgcgccga gcacatgcat aaaagcccct
    15841 aacaactaaa agttgtaagg aaggagaggg atacatgcgc cgagcacatg cataaaatcc
    15901 cctaacaact aaaagttgta aggggattta aattaattta gtgtatcttg gatatcttgt
    15961 tttgtttgat taatatcttc tgttttttct tcttttttat cttttaattt ttcttcaact
    16021 tctttagctt tttctgctgc ttttttattt tgattttcat tagacatgat taattcctcc
    16081 caaattggat aattatttat atataaatct tacccggttg tactttcgtt aaacttttct
    16141 aagtctatag cactatttat tcatttatct aaagacaaca acattagatt aatatataat
    16201 gattttgagg tgaacataat gtcttttctt aggaaacacg ccgaaattat ttttagctat
    16261 ttaatcggta tcgtttcact cttcactggt ctcattattt taattaactt gccattaatt
    16321 aaacaattaa atggtggtaa aaaagttgat acacatgttc ataatgtgtg ggaatttctg
    16381 aatgcatttt tcagtgaaat tattaaagta atgagtcgat ttataggtaa tttccctata
    16441 gttagtgcaa ttgtgataat tatattcggt attttagtta tgttgattgg tcatacatta
    16501 cttagaacta ttaagtatga ctatgatatt tctatctttt tcttagttat cggtatcatg
    16561 tactttatta ttactcttat attaatgact caagtttatg gattctttgc agtgattttc
    16621 attattccat ttacaattca tataggatat atcgtctata aagatgaatt gaatcaggaa
    16681 aatgtaaaaa atcatttcat gtggataatt gtgagttatg gtataagtta cttaattaca
    16741 caaattgcat tgtatggcag aattgatgct aatgaaatag agtcaattga tatcttaagt
    16801 gtcaatgctt tctttataat tatgtggtta cttggtcaaa tggctatttg gaatttcttg
    16861 ttcttgcgcc gagctttacc tttaacaaag caagaattag gtgaagagga gccagaatta
    16921 tcaagaacaa gtaaagggaa tgtcacgaat caaactaaaa ttcacttgaa acaactccaa
    16981 gataagacta cagaatatgc acgtaagaca agaagaagtg tcgatttaga taaaattaga
    17041 gctaaaagag ataaattcaa aaagaaagtt aatgatatta tcgatattca agaagacgat
    17101 attcctgatt ggatgagaaa accgaaatgg gttaaaccaa tgtatgtcga actattttgt
    17161 ggtgtcgtca tctttttatt cacattttta gaatttaata atcgtaatgc attatttgta
    17221 tctggtgatt ggaaattatc acagacacaa tatgttattg aatgggttac attattaatt
    17281 ctgttattca ttattatcgc atatatcgct acaacgttaa ctttccactt gaaaggtaag
    17341 ttttattatt tacaattatt tatggggagc attttattct ttaaattgtt aacggaattt
    17401 ataaatataa tgattcatgg actattactt tcagtgttca ttacgccaac attactatta
    17461 atgttattgg caatcatcat ttcttattcg ttacaattac gagagcgacc ataattaaaa
    17521 gcattataaa agtactatct attaaacatt ttgatgtgta cgctataagt tagatatatc
    17581 tctaacttac ttagatacag gtcaatgaag tttatggata gtactttttt tgtaactaga
    17641 tttgattgat tcaggtgatg tgaattaagt attgaaaatt gtatacaaag ttttagtgca
    17701 aataaaatag ttgaaaagtt atccatttgt aaaatcaaga aaactagtaa atagttgaag
    17761 cgacttatgg aatttgcgaa acgatatata gtatttcctt tgtagaaatt taacatatat
    17821 cattcgaatt actaatttgt taaaatcaac agtaagatta gaagtagatg atattgaaat
    17881 ttggcaaaca atttaatcta tataaaacta caacgaaaca cagaaaggaa gttgtcagat
    17941 gaaaatagca actctgaaca aaggcaaaga aacaaaatat tttaatggat atcctttaat
    18001 tgaagaagag gatatctatt cacaagatca tttaaaagaa ggagatattt ttcaaattgt
    18061 gactgataaa tcacaatatg tcgcaacggc ttatgttggt cgccaacata aaggattagg
    18121 ttgggttcta acatacgata aagctcaaca aatcaacaca gctttctttg taaaattgtt
    18181 taatactgca ttagcagaac gtgattatta ttttaatata gatggaacaa atgcttttag
    18241 attatttaat gctgaaggtg atggtgttgg gggattaaca atcgacaatt acgatggtca
    18301 tttgttgatt caatggtact caaaaggtat ttataaattt aaatatgcca ttcttgaagc
    18361 ggttagaaaa gtatttgatt ataaatctat ttacgaaaaa gtaagattta aagacagcga
    18421 atatagtggt ggttttgttg aaggagatgc acctgagttt ccaattgtta tcgaagaaaa
    18481 cttcacattt tataatgtag accttgaaga tggtttgatg acaggtatct ttttagatca
    18541 aaaagaagtg cgcaagaaat taagggatca atatgccaaa gaacgccatg ttttaaactt
    18601 atttagttat acaggtgctt tttctgcaat agcagcaagt gaggcatctt caacaacaag
    18661 tgtagatttg gctaatcgtt ctcgtagttt aactgaagaa aattttggat taaatgctat
    18721 tgatcctaaa tcccaatata tttatgtcat ggacactttt gatttctata aatatgctgc
    18781 acgacatgga catagttatg acacgatcgt gattgatcca cctagctttg cgcgtaacaa
    18841 aaaacgtaca ttttcagtgc aaaaagatta tgacaaatta attaatggcg ccttaaatat
    18901 cttatcatct gaaggaacat tattgttatg tacaaacgca agtgtatatc cattaaagca
    18961 atttaaaaat actattaaaa agacgcttga agagagtggc gttgattatg aattaactga
    19021 agttatggga ttaccaaaag attttaaaac gcatccacat tataagccat ctaaatattt
    19081 aaaagctgtt tttgtaaata ttagacatta atttgataaa aaggggtata gtaacttatt
    19141 gagaaaaaga agggtgataa tattatggga ttcaaaaaca atttaacatc aaatttaaca
    19201 aataaaatcg gtaattcagt ctttaaaata gaaaatgttg acggaaaagg tgcaatgcca
    19261 acgacgattc aagaattgag agaaagacga caacgtgctg aagcaattgt aaagagaaag
    19321 tctttaatgt catcaacaat gagcgttgtt ccaattccgg gtttagattt tggtgttgat
    19381 ttaaaattaa tgaaagatat tatcgaagat gttaataaaa tttatggttt agatcataag
    19441 caagttaata gccttgggga tgatgtgaaa gaaagaatta tgtctgcagc agcaattcaa
    19501 ggtagtcaat ttattggtaa aagaatttca aatgcatttt taaaaattgt aattagagat
    19561 gtagctaaac gtactgctgc aaaacaaaca aaatggtttc ctgttgtagg acaagctgtg
    19621 tctgcatcta ttagttacta ttttatgaat aaaattggaa aagatcacat tcaaaaatgc
    19681 gaaaatgtta ttaaaaatgt catgtaggtg ctataatagt tttgcaattt gcaaatttta
    19741 ctgaaaccgg ttttaaacga attgaattta aagcatggtt ttggtaaagt taatgtataa
    19801 aactaagtta gtattgtaat aatattgaag attctaacta tacgaaggag aaatgtaatt
    19861 atggaacaaa attcatatgt aatcatcgac gagactggta ttcacgctag accagcaaca
    19921 atgttagtac aaacagcttc aaaattcgat tctgatattc aattagaata taacggtaag
    19981 aaagtaaact taaaatcaat catgggtgtt atgagccttg gtgttggtaa agatgctgaa
    20041 attacaattt atgctgacgg tagtgatgaa tctgacgcca ttcaagcaat cagtgacgtc
    20101 ttatcaaaag aaggattgac taaataatca tgtctaaatt aattaaaggt attgccgcat
    20161 ctgatggtgt cgcaattgct aaagcttatt tattagttga gccagactta acattcgaca
    20221 aaaatgaaaa agtcactgat gttgaaggag aagttgcaaa gttcaatagc gctatcgaag
    20281 cttctaaagt tgagttaact aaaattagaa ataatgcaga ggttcaacta ggtgctgata
    20341 aagctgctat ctttgatgca catttattag ttttagatga ccctgaatta attcaaccaa
    20401 tccaagataa gattaaaaat gaaaacgcta atgctgctac agcattaacg gatgtaacaa
    20461 cacaatttgt tacaattttt gaatctatgg ataacgaata catgaaagaa cgtgcggctg
    20521 atattcgcga cgtttctaaa cgtgtgttat cacatatttt aggtgtagaa ttaccgaatc
    20581 cgagtatgat tgatgaaagc gttgttattg tagggaatga cttaacgcca tctgatactg
    20641 ctcaattaaa taaagaattc gtacaaggtt ttgctacaaa cattggcgga agaacaagtc
    20701 actctgcaat tatgagtcgt tctttagaaa ttccagcaat tgttggtaca aaatcaatta
    20761 ctcaagaagt taaacaaggc gacatgatta tcgtagatgg attaaatggt gatgtaatcg
    20821 ttaatccaac tgaagatgag ttaatcgctt atcaagataa acgtgagcgt tattttgctg
    20881 acaagaaaga attacaaaaa ctacgtgatg ctgatactgt tacagttgat ggtgttcacg
    20941 cagagcttgc tgcaaatatt ggtacaccta atgatttgcc aggtgttatt gaaaatggtg
    21001 cacaaggtat cggcttatat agaactgagt ttttatatat gggtcgtgac caaatgccta
    21061 cagaagaaga acaatttgaa gcttataaag aagtattaga agcaatgggc ggtaaacgtg
    21121 ttgttgtacg tactttagat ataggtggag ataaagaatt atcatactta aacttgcctg
    21181 aagaaatgaa tccattctta ggttaccgtg cgattcgttt atgccttgcg caacaagata
    21241 ttttcagacc acagctacgt gcattattac gtgcatcagt ttatggtaag ttaaatatca
    21301 tgttcccaat ggttgcaaca attaacgaat ttagagaagc taaagctata ttattagaag
    21361 aaaaagaaaa ccttaaaaat gaaggtcatg acatttcgga tgatatagaa ttaggaatca
    21421 tggtagagat acctgcaaca gcagcattag ctgatgtctt tgctaaagaa gtagatttct
    21481 tcagtatcgg tacaaatgat ttaattcaat acacattagc tgctgaccgt atgtcagagc
    21541 gtgtatcata tctataccaa ccatataacc cttcaatctt acgtttagtt aaacaagtta
    21601 ttgaagcgtc acataaagaa ggtaaatgga caggtatgtg tggtgaaatg gctggagatg
    21661 aaacagctat tccattattg cttggtttag gtttagatga gttctctatg agtgcaacgt
    21721 ctattctgaa agcaagaaga caaattaatg gtttaagtaa aaatgaaatg actgaacttg
    21781 ctaaccgtgc agtcgactgt gcaacgcaag aagaagttat tgaattagtt aacaactacg
    21841 taaaataatt aattctttcg aaagacagaa ttataaataa acccgaagta ttcattgtaa
    21901 gtaattatga atacttcggg ttctttttta tgatttatgt taatttcaat ggaatgttgt
    21961 tgctaactaa ttaaacaact atgtgtcatt aacaaaatgt agttctttaa ttaatgttag
    22021 ttaatttaaa aatcgatatt taatttatga tagctataaa tgatgaagtt gatttagatg
    22081 aaaattaatc ttggatattt aatactttgt tgatttcatc aagatcaaca tggtacattg
    22141 gattgccatt caacaaaata aacggagttg aaaaagcatc aaaatctatc atttcgtttc
    22201 gatattgttg attgttgata tttctctctt caaaatcaat gtgatgctca tttagataat
    22261 tttttacaaa tgtacaaggt ggacaatcat tctgcgtata aacgattatt tctgacatgt
    22321 atcatgctcc ttcgtgcttt attctaatgt aatatacaca attatacatt gaaattcaaa
    22381 tttgtgaaca cattgtgaac tgacataaat tatacacaat ttcatagcgc ataatgtttt
    22441 aaaaatacac attatagcaa ttataattaa gttcattgtg ttgtcatcaa aaaaagaaaa
    22501 aggtgatgtt tttaaatgga tacagttgaa atcagtcggt ttttgacagc tatgacttta
    22561 gcagttcata tcatttttgc aacgattggt gttggtatgc ctttaatgtt cgcaattgca
    22621 gaatttttag gtattcgcaa aaatgatctt caatatatag ctatggccaa aagatgggct
    22681 aaagcttata caattactgt agcagtggga gttgttacag gtacaattat aggacttcaa
    22741 ttatcattga tttggcctac atttatggaa atgggtggac acgttattgc acttccatta
    22801 tttatggaaa catttgcgtt cttctttgaa gctattttct taagtatata tttatatact
    22861 tgggatcgtt ttaaaaataa atggacacat ttcttaatta gtataccagt aattattggt
    22921 ggctctttct cagcattctt cattacttca gtgaattcat ttatgaatac gcctgcaggt
    22981 tttgagttga agaatggaaa gatggtcaat gttcaaccta tagaagcgat gtttaaccca
    23041 tcgtttatag ttcgatcatt tcacgtaatt actacagccg gtatgacgat ggcatttgtt
    23101 attgcatcaa tagcagcttt taaattattg cgtaatcgtc aacctaaaga tactgtctac
    23161 cataagaaag ctttgaaaat gtctatgata gttggattct tttcaacatt actttctatg
    23221 ttggcagggg atttatctgc aaaatttttg cataaattcc aacctgaaaa attagcagct
    23281 tatgaatggc atttcgatac atcttcccat gccaaattat tattatttgg tgtgttagat
    23341 gaaaagactc agcaagttaa aggtgcgatt gaattacctg gactattgag tttcttagca
    23401 gataatagtg tcaaaactaa agtacaaggg ttaaatgatt ttccaaaaag tttacatccg
    23461 cctatgatcg tccattattt ctttgattta atggtaacga tgggaatttt atgttttgtc
    23521 atttcaggtg tttatgtctt aactttaatg tttaaaaagc ttagaaagtt ttctactcat
    23581 aaatggatgc tttatggaat attattaaca ggcccagctt caatgctagc tatagaattt
    23641 ggatggttct taacagagat gggtagacag ccttggattg ttcgtggtta tatgcgcgtg
    23701 gcagaagcag caacacaagc aggcggaata accttcgtta caattttatt tggcatattg
    23761 tacatcattt taatgtatac atgtgcatac gtattaattc gtatgtttaa aaataaaccg
    23821 gcgtatgaag atgtaaatcg tttagccaag aagcaaggag gagaaataga aaaatgattt
    23881 atgcatttat aggtataaca gtgttatggt tgtttttatt ttgctatatc attattgctt
    23941 ctatagattt tggggcaggt ttcttcgcat tgcattcaaa gttaactggt gatgaaaaga
    24001 aaattaatca cttaatttca cgttatttaa acccagtttg ggaagttacg aatgtattct
    24061 ttgttttctt cttcgtagga ttcgtaggtt tctttccaga gtcaatcaaa tatttaggca
    24121 cggtattgtt aataccaggt tcaatagcac tgattatgat atcgttgaga aacagttttt
    24181 atgcatttga aaattatggt caagatacaa aattagcatg gatgatcatg tatggggtaa
    24241 gtggattatt aattccagct tcattatcta ctgctttaac tattacagaa ggtggctata
    24301 ttaatgttcg aaacaatgtt atcgatctag attgggtgca gttattatta agcccatttg
    24361 cttggtctgt agtattcttg gcaattattt cagttttata tatttcatca ggatttttga
    24421 catattatgc taaaaaagca aatgacgaac cagcatataa tttaacaaga caatggcaca
    24481 tatttttagg gccgccgatg attatcattt gtttattcgt atttctatca ttacgcattc
    24541 aaaattctga acatttttat tcagctgttt ttgactattg gtggatgttt ggtataagtt
    24601 tcttattctt tgcattagct tcattgttaa cattctttaa gaaaaaacat ggtttggctt
    24661 ttgtatttgt tattttacaa atgatgttcg cgttctttgg ctatggaatt agtaaattgc
    24721 catacctttt atatccgttt gtaaaaatta cagatgcata tgttaatcca gaaatgggct
    24781 ggacattagt gattgtcttt attttaggtt tacttttatt acttccatcg ttaatattat
    24841 tattaagatt atttgttttc gacaaagaat atgttgaagg aaagaaatca taagttgaga
    24901 cgatacccca actttcattt acttgcatta ctgcatttag ataatttagt tggggtactc
    24961 atttataata attttaaatt tatattttca atgaattgat gaaaatcatt taatggaggg
    25021 cgagtatggg taaagaatat gtagtcatcg gtctaggtcg ttttggaggt agtatcgttc
    25081 gtgagttgaa tgcattagac atggatgtaa tggccatcga ccatgatgaa aatagagtga
    25141 atgaatatag tgatatcgct actcatgcgg ttgttgcaga cactacagat gaagcagtta
    25201 tgaaaagttt aggtatccgt aactttgatc atgtcattgt ggcaattggt gaaaatattc
    25261 aatcaagtac gttgacgact ttaattttaa aagagttagg tgtaaaaaaa gtaactgcta
    25321 aagcacaaaa tgattatcat gcaaagattt taaataaaat tggagcagat acggttgtgc
    25381 accctgagcg tgatatgggt agacgtattg cgcataatgt tgcgagtgca agtgtacttg
    25441 attatcttga gttggcagac gagcattcta ttgtagaatt gaaagcaact gaaaagatgg
    25501 cggggcagtc tatcattgat ttagatataa gagcacaata tggaattaac attattgcaa
    25561 ttaaacgagg caaagagttc atcatttcac caaatccaaa tattaattta gaaataggtg
    25621 atattttaat catgattgga catgataatg atttaaatcg ctttgaaaaa aatattgcga
    25681 cgagataatc gggagtggga cagaaatgat attttctcaa aatttatttc gtcgtcccac
    25741 cccaacttac attgtctgta gaaattggga atccaatttc tctttgttgg ggcccacacc
    25801 ccaactcgca ttgcctgtag aatttctttt cgaaattctc t
//
LOCUS       DALAAR010000037        25535 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000037 DALAAR010000000
VERSION     DALAAR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.039"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            90..317
                     /locus_tag="O2458_001542"
     CDS             90..317
                     /locus_tag="O2458_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887408.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(622..1569)
                     /locus_tag="O2458_001543"
     CDS             complement(622..1569)
                     /locus_tag="O2458_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCZ3887409.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            1818..2006
                     /locus_tag="O2458_001544"
     CDS             1818..2006
                     /locus_tag="O2458_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ3887410.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(2481..2554)
                     /locus_tag="O2458_001545"
     tRNA            complement(2481..2554)
                     /locus_tag="O2458_001545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2518..2520),aa:Arg,seq:tct)
     gene            2918..3052
                     /locus_tag="O2458_001546"
     CDS             2918..3052
                     /locus_tag="O2458_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ3887411.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            3109..3243
                     /locus_tag="O2458_001547"
     CDS             3109..3243
                     /locus_tag="O2458_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ3887412.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            3367..4062
                     /locus_tag="O2458_001548"
     CDS             3367..4062
                     /locus_tag="O2458_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCZ3887413.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(4171..4611)
                     /locus_tag="O2458_001549"
     CDS             complement(4171..4611)
                     /locus_tag="O2458_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3887414.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            4806..6419
                     /gene="bshC"
                     /locus_tag="O2458_001550"
     CDS             4806..6419
                     /gene="bshC"
                     /locus_tag="O2458_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCZ3887415.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            6563..6994
                     /gene="mraZ"
                     /locus_tag="O2458_001551"
     CDS             6563..6994
                     /gene="mraZ"
                     /locus_tag="O2458_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCZ3887416.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            7010..7945
                     /gene="rsmH"
                     /locus_tag="O2458_001552"
     CDS             7010..7945
                     /gene="rsmH"
                     /locus_tag="O2458_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCZ3887417.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            7959..8360
                     /gene="ftsL"
                     /locus_tag="O2458_001553"
     CDS             7959..8360
                     /gene="ftsL"
                     /locus_tag="O2458_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCZ3887418.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            8341..10575
                     /locus_tag="O2458_001554"
     CDS             8341..10575
                     /locus_tag="O2458_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ3887419.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            10867..11832
                     /locus_tag="O2458_001555"
     CDS             10867..11832
                     /locus_tag="O2458_001555"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCZ3887420.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            11834..13183
                     /locus_tag="O2458_001556"
     CDS             11834..13183
                     /locus_tag="O2458_001556"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCZ3887421.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            13199..14518
                     /locus_tag="O2458_001557"
     CDS             13199..14518
                     /locus_tag="O2458_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCZ3887422.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            14624..16036
                     /gene="ftsA"
                     /locus_tag="O2458_001558"
     CDS             14624..16036
                     /gene="ftsA"
                     /locus_tag="O2458_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCZ3887423.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            16069..17241
                     /gene="ftsZ"
                     /locus_tag="O2458_001559"
     CDS             16069..17241
                     /gene="ftsZ"
                     /locus_tag="O2458_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCZ3887424.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            17501..18292
                     /gene="pgeF"
                     /locus_tag="O2458_001560"
     CDS             17501..18292
                     /gene="pgeF"
                     /locus_tag="O2458_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCZ3887425.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            18316..18984
                     /locus_tag="O2458_001561"
     CDS             18316..18984
                     /locus_tag="O2458_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCZ3887426.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLKGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            18981..19544
                     /locus_tag="O2458_001562"
     CDS             18981..19544
                     /locus_tag="O2458_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCZ3887427.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            19556..19846
                     /locus_tag="O2458_001563"
     CDS             19556..19846
                     /locus_tag="O2458_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCZ3887428.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            19956..20735
                     /locus_tag="O2458_001564"
     CDS             19956..20735
                     /locus_tag="O2458_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCZ3887429.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            20759..21376
                     /locus_tag="O2458_001565"
     CDS             20759..21376
                     /locus_tag="O2458_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCZ3887430.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            21597..24350
                     /gene="ileS"
                     /locus_tag="O2458_001566"
     CDS             21597..24350
                     /gene="ileS"
                     /locus_tag="O2458_001566"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCZ3887431.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            24640..25437
                     /locus_tag="O2458_001567"
     CDS             24640..25437
                     /locus_tag="O2458_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3887432.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         9428 a         3490 c         4814 g         7803 t
ORIGIN      
        1 tcgtggtaaa tatgtatttg ctgtaaacag tttttgtaac acatgataca tatggttgtg
       61 aaaatagcat atataaagga tggctataaa tgacacattt gacaaaggtt ttagatacac
      121 taactggaat atgcgtagta ttattattta gtaaatattt tgtggcgtat gcaaatatgg
      181 tgtttgattg gaatttaaga tggtatttgc tagaaaacat accacatttg ccaattatat
      241 tatttattct gatgtttatt ttcggagtac cttctgaaat gataaaagat aggcaaagga
      301 aaaataacgg tgtttaattt atcgatattt agaggtgata aaatttgtca actattacaa
      361 agactaaaat aaaaatcttt attaattatt ttattggtat attagccttg gcgtttgctg
      421 cttatatatt atttaaataa agtttagagt gaaacgtgtt tatgaactag acgttctagt
      481 atagttacta tagctttagt cagaaggtat cattgataag atcatattaa atcaaagagg
      541 cattgatata cactaaaaag aggcaagatt acctgcctct ttttagttat taaatatacg
      601 tgttaattct tggtaatagt gttactgaga cgcattacga agaaatttat cttgtttttc
      661 cttttttaaa aagaagtgaa gatatcctat aaagactcta agtactatta cagtggctaa
      721 taacacttcg ataaacaaaa gacctttcca aatgtctgga aacataagta caggcaaact
      781 gttctttaaa gcagttgctg agattactaa agggaatgtg aaagctgaaa atacgggcga
      841 aaaaggttct tttagtaatt taggcaattg tataatgata taaaaataaa atatttgagc
      901 taatagtaaa aatataatta cgataaaagc attcgcctta ggaaaagcta taacatatgc
      961 tgcagcgact aaagaaaatg gtgcacaaat tgtcgatgtg ttcggtttga ttgacgtctg
     1021 caatggaaat gcttttaatc gcttgaaaac tataggtaag acaatacaag tagctacaaa
     1081 gccatatatt actgttaatt gacctatgaa aaaatatccg ctaacgggtg ccgtcaatcc
     1141 tgcgatagca ataccaataa aaagtacagt ccacgaagga taaacatttt taagtgaaaa
     1201 atcttttaaa tattttattg aaaaaataat catatgcgtc ataattccca caaggcataa
     1261 aatccaaata ggcgttatta agctattgat aaaagttatg ttactaaaaa atgtatttaa
     1321 ataagtagta cctaaaaagc cagacatgaa aaatgttgtg aacactgatg aaactagagg
     1381 actgtttaat tgttctttca cattattaaa atatttgatc atagtacata aaaggtgaat
     1441 ccaaatcaag aaagcaaaga ttccgcaaac agcgtttaaa gtaagagata agtcttttaa
     1501 tagattaccc aggcctaata atcctaagac tagtcctgac gttactagag gtgctttttg
     1561 aagtctcatg atttacaacc tttcttatgt gattttcttc actaattata tcatgatagc
     1621 tatggccaat taataagaag agtgtgtact attacgttat tagagtatgt atattggatt
     1681 gatagacaca taattgacat ttaaatctca aaattaatga tatttgaggt atgctttgca
     1741 acctaaatat tggaatatgt ggaaaagtaa ttatttaatt taaatgatga ttgaagattt
     1801 acaagggggt gtacaaaatg agaaatcaaa ttcaaaaact attagacagt gatttgagca
     1861 gtttacatat atcgaaacaa acaggagttc cacaaagcac aatacacaga atgagaaaaa
     1921 atgaaagatc attagacaat atgtcattga aaaacgctga actactttat aaatttgcca
     1981 atagtatatt tagcaatgaa aattaagaag aataattagt gagttaaata attaaaaaac
     2041 cacgtctcga tggacgtggt tatttttttt agatgtatat aattataatt ataggatttc
     2101 gggcccctga aaagtcccta aaattttgaa tagtatcaaa atctatagga ataatataat
     2161 aataaaaatc tctacgcatg caaatggtga gtgttaaaaa tcttggtgta gtgttggtgt
     2221 agttcaggtg cagtatagat gaaatttgag cacaaaatta cttaattaaa aatgtataaa
     2281 caagtgtcag acgcgtgtca attgtgtgtc aaaaaattct ataacgaata agcatgcttg
     2341 ttttaattac tgtattttcg gggcttatgc gggggacgtt ttggtgacgc atcatactat
     2401 attactgaaa ttcaaaacaa aaagagcccc gtaatcacgg aactcttttg tttggtaatg
     2461 cgtataaaaa tacctataaa cgtcctggga gggattcgaa cccccgaccg atggcttaga
     2521 aggccattgc tctatccagc tgagctacca ggacacgttt aacaacacaa gaattattat
     2581 atctaaatga acttaaatta gcaatacctt gtaaataaaa aatgtttata tttttcacta
     2641 ttatagagct atttatctaa aaaggttcaa taagacttaa atacgaattc aggcaactta
     2701 attgtgttaa atacagtttt gaatgcctaa ctgtatttct tttctcttta aaatacagtt
     2761 aagtacatta taagatgttg tgcggataaa caaactaatt gtatcaaatt tattttaaaa
     2821 taacaacaac aaaacgataa gcgaataaca tttcggtgat ttaaaagcta cgcacgtttt
     2881 tgttatcttc aaatttaaat tttaaggagt gttttcaatg gaaggtttat ttaacgcaat
     2941 taaagatacc gtaactgcag caattaataa tgatggcgca aaattaggca caagcattgt
     3001 gagcatcgtt gaaaatggcg taggtttatt aggtaaatta ttcggattct aatttcaata
     3061 tgttatgtaa gtaatcagta ttatttcaaa ggtgagggag agatttaaat gactggacta
     3121 gcagaagcaa tcgcaaatac tgtgcaagct gcacaacaac atgatagtgt gaaattaggc
     3181 acaagtatcg tagacatcgt tgctaacggt gtgggtttac taggtaaatt atttggattc
     3241 taatataata actaatattc tttaaaataa actgggtgag catactttaa tgttatgcac
     3301 tcagtttatt ttatttgcag aaatttgagc ctctgttaag atttagatac atagacaata
     3361 taggagatgg ggaaattggg atataaaaat attttgatag actttgatga tacaattgtt
     3421 gatttttatg atgcagaaga atgggcgttt cactatatgg cgaatgtttt taatcataaa
     3481 gcaacaaagg atgatttttt aacatttaaa aaaatcaatc accaacattg ggaagctttt
     3541 caacaaaata aattaacgaa gtctgaagta ttatcagaac gatttgtgaa ttacttcaaa
     3601 catcatcaaa tggaagttga tgggcatcgt gcagatgtgt tatttagaaa tggattagca
     3661 gaagctaaag ttaaatactt tgatcaaaca ttagaaacaa ttgtcgaatt atcgaaaaga
     3721 catgatttat atattgttac taatggtgta accgaaacgc aaaagagaag gttaaatcag
     3781 acgccgttgc ataaatatat taaaaagata tttatatctg aagaaacagg atatcaaaaa
     3841 cctaatccgg aattttttaa ttatgttttt aatgatattg gtgaggatga aagacagcac
     3901 tcgattatag ttggagattc tttaacatct gacattctag gtggaatcaa tgcgggtata
     3961 gctacttgct ggtttaattt tagaggattt gatcataatc caggaattat acctgattat
     4021 gaaattaatt catggaaaca actaaatgat attgtacgtt aagacaaata atttgaaaat
     4081 gatttactta tattagctta agaaagtaat tgtaaaaaag acactacgaa aagatgcatt
     4141 ttcatgctcg tagtgtcgaa tattattatc ttatggtttc cacaataaga catcatgtcc
     4201 ctctatattt ttagcttcca catctgtaaa tccatgatgt aaaaagaaat ccttagaatc
     4261 atttctgcca atggctttaa ttggcatatt gaaactcttt gcaaaatcaa tcaattctga
     4321 agcgtaccct ctgttttgat attttggtaa tacttctaac ttccataata atatataatc
     4381 ttcaaaatct gggaagtaga tttcttcgac atcacctttt tttagtaatg ccattctagc
     4441 tcctaattga tctccgacaa atatgccata aaatggtgaa tctgaacttg catcaatcat
     4501 ttgaccgttt aactcattga ccatgtataa gtctttgttg ccaaacgctc taaagttttc
     4561 gaataattcg tcagttttgt aattaatttc aagacgtttg atttcactca tatttatcta
     4621 ccccttattt gtttactact attattatac attaaaccac ttgtgttttt cattgatttg
     4681 aaaatgtaaa acaatgaata ttataaattt tccatgatta aattgatttt caagccgtaa
     4741 aattgtcaat caccggtata gactttatac tatagtttgt aatcgcgtac ttaaggagaa
     4801 aaaacatgga ctgtaaagta gttagtttaa atgaaaaaga tcagtttata ccaaaaataa
     4861 agagcagtga ccctgtaata acaggattat ttcaatatga tgcagctcaa caaactagtt
     4921 ttgaaaaaag gatgtctaaa gaaaataatg gaagagaagc ggcattagcg aatgttattc
     4981 gtgaatatat gagtgattta aagctttcaa gtgaacaaga attaaacata caacatttag
     5041 ctaatggttc aaaagttgtg attggtggac aacaagcagg gcttttcggg ggaccattgt
     5101 atacattcca taaaatattt tcaatcatta ctttatctaa ggaattaacg gatacacata
     5161 agcaacaagt agtaccagtt ttttggattg caggagaaga tcatgatttc gatgaagtga
     5221 atcatacatt tgtttataac gaaaatcatg ggtcgctgca taaggttaaa tatcatacaa
     5281 tggagatgcc agagacgact gtctctagat attatcctga taaggctgag ttgaaacaaa
     5341 ctttaaaaac gatgttcatt catatgaaag aaactgttca tacacaaggt ctactggaga
     5401 tttgtgacag aattattgac caatatgact cgtggactga tatgtttaaa gcactactgc
     5461 atgaaacatt taaagcatat ggcgttctat ttatagatgc gcagtttgag ccgttaagaa
     5521 aaatggaagc gcctatgttt aaaaagattt tgaaaaaaca tcagttgctt gatgatgctt
     5581 ttagagcaac acaacaacgt actcaaaatc aaggcttgaa tgcgatgata caaacagata
     5641 caaatgttca tttattctta catgatgaaa atatgcgtca attagtttcg tatgatggta
     5701 agcattttaa attaaataaa acagataaga catatataaa ggaagaaatt ataaatattg
     5761 cggaaaatca acctgaatta ttttctaata atgtagtgac aagaccatta atggaagaat
     5821 ggttatttaa cacggtggca tttgttggag gaccgagtga aattaagtac tgggctgaac
     5881 taaaagatgt atttgaacta tttgatgttg aaatgcctat cgtgatgcca aggcttagaa
     5941 ttacttattt aaatgaccgt atagaaaaat tactttcgaa atacaatatt ccattagaaa
     6001 aagtgttagt cgatggtgtt gaaggagaaa gaagtaagtt tattagagaa caagcatcac
     6061 atcaatttat tgaaaaggta gaaggtatga ttgaacaaca gcgtcgtcta aacaaagact
     6121 tattagatga agtggcgggg aatcaaaata atattaacct tgtgaataaa aataatgaaa
     6181 ttcatataca acagtatgat tatttgttaa aacgttatct tttaaacatt gaaagagaaa
     6241 acgacatcag tatgaagcaa tttagagaaa ttcaagaaac actccatcca atgggaggat
     6301 tacaagaaag aatatggaat ccacttcaaa ttttgaatga ttttgggaca gatgtgttca
     6361 agccctccac ctatccacca ctttcttaca cttttgatca tattattata aaaccttaat
     6421 ataccaaggg tttagcccga tttatcttaa tgataaatcg ggcatttttt tgttttttaa
     6481 aataaatttc acaaattttg tataaatagt ggtggatagt ggggagatgt ggtaaattat
     6541 atataaggtg aggtgataaa aaatgttcat gggagaatac gatcatcaat tagatacaaa
     6601 aggacgtatg attataccgt ccaagtttcg ttatgactta aatgagcgtt ttattatcac
     6661 aagaggcctt gataaatgtt tattcggtta cactctagac gaatggcaac agattgaaga
     6721 gaaaatgaaa accttaccta tgacaaaaaa agacgcacgt aagtttatgc gtatgttctt
     6781 ctctggtgct gttgaagtag aacttgataa gcaagggcgt attaacatcc ctcaaaactt
     6841 gaggaaatac gctaatttaa ctaaagaatg tacagtaatc ggtgtttcaa atcgtattga
     6901 gatttgggat agagaaactt ggaatgattt ctatgaagaa tctgaagaaa gtttcgaaga
     6961 tattgctgaa gatttaatag attttgattt ttaaaatgga ggaattgaag tgtttcatca
     7021 tatcagcgtt atgttaaacg aaaccattga ttatttaaat gtaaaagaaa atggtgtgta
     7081 cattgactgt acgctaggtg gagcgggaca tgccctttat ttactaaatc aattaaatga
     7141 cgacggaaga ttaatagcaa tcgatcaaga ccaaactgca attgataatg ctaaagaggt
     7201 attaaaggat catttgcata aggtgacttt tgttcatagc aacttccgtg aattaactca
     7261 aatattaaaa gacttaaaca ttgaaaaagt agatggaatt tattacgact tgggtgtttc
     7321 aagcccacaa ctcgacattc cagaacgagg attcagttat caccatgacg caacattaga
     7381 catgcgtatg gaccaaacac aagaactaac agcatatgaa attgttaaca attggtcata
     7441 tgaagcgtta gtgaagattt tttatcgcta tggcgaggag aaattttcaa aacagatagc
     7501 tcgaagaatc gaagcacatc gcgaacaaca accaataaca acaacattag aattagttga
     7561 cattataaaa gaaggtattc ctgcaaaagc aagaagaaaa ggcggacatc ctgcaaaacg
     7621 agtatttcaa gcactacgaa ttgcagtaaa cgatgaattg tcagcttttg aagattcaat
     7681 agaacaagcg attgaattag tgaaagtaga tggcaggatt tcggtaatca ctttccattc
     7741 tttagaagat cgtttatgta aacaggtgtt ccaagaatat gaaaaaggtc cagaggtacc
     7801 aagaggatta ccagttatac cagaagcata tacacctaag ttaaagcgtg ttaatcgtaa
     7861 accgattacc gctacagaag aagatttaga tgacaataac agagcacgaa gcgcgaaatt
     7921 acgtgtagct gaaatactta aataaggagc aatttataat ggctgtagaa aaagtgtacc
     7981 aaccatatga cgaacaagtt tataatagta taccgaagca acaaccacaa actaagcccg
     8041 aaaagaagac tgtttcgaga aaagtggttg tacaattaac taaatttgaa aaagttttat
     8101 acataacttt gattactgta attgctatgt taagtattta tatgctatct ttaaaaatgg
     8161 atgcgtatga tacgcgagga aagattgcag atttagatta taaaatagat aaacaatcaa
     8221 gtgaaaacag tgctttacaa tctgaaatca aaaagaattc ttcttatgaa cgcatatacg
     8281 aaaaggctaa gaaacagggg atgagccttg agaacgataa tgtaaaggta gtgcgtagta
     8341 atggcgaagc aaaaaattaa aattaaaaaa aataaaatag gggcagtcct acttgttggt
     8401 ttattcggac tgctcttttt tatattggtt ttaagaattt catatatcat gattactgga
     8461 cattctaatg gtcaagattt agtcatgaag gcaaatgaaa agtatttagt taagaatgca
     8521 caacaaccag aacgaggaaa gatatatgat cgtaatggta aagtgctagc agaagatgta
     8581 gaaagatata aacttgttgc agtaatagat aaaaaggcga gtgccaattc taaaaaacct
     8641 aggcatgtag ttgataaaaa agagactgca aagaaattat ctacagtcat taatatgaag
     8701 ccagaggaaa ttgaaaagag acttagtcaa aagaaagctt tccaaattga atttggacgc
     8761 aaaggaacaa atttaacgta tcaggacaaa ttgaaaatag agaaaatgaa tttgcctggt
     8821 atttctttat tgcctgaaac agaacgcttt tatccaaatg gcaattttgc atcacactta
     8881 attggtagag ctcagaaaaa tccggatact ggtgaactta aaggtgcact tggagttgaa
     8941 aagatttttg atagttattt aagtggatct aaaggatcat tgagatatat tcatgatatt
     9001 tggggatata tcgcaccaaa tactaaaaaa gagaagcagc ctaaacgtgg tgatgatgtc
     9061 catttaacaa tcgattcaaa tattcaagta tttgttgaag aagctttaga tggcatggtt
     9121 gaaagatacc agccgaaaga tttatttgcg gttgtcatgg atgccaaaac tggagaaatt
     9181 ttagcataca gtcagcgacc aacatttaat cctgaaactg gtaaagactt tggtaaaaag
     9241 tgggcaaatg acctttatca aaacacatac gagcctggat caacatttaa atcatatggg
     9301 ttagcagctg ctattcaaga aggtgctttt gatcctgata agaaatataa atctggacat
     9361 agagatatta tgggttcacg tatttcagac tggaatagag tcggttgggg tgaaatccca
     9421 atgtcactcg gatttactta ttcatctaat acattgatga tgcatttaca agatttagtt
     9481 ggtgcagaca aaatgaaatc ttggtatgaa cgatttggat ttggaaaatc aactaaaggt
     9541 atgtttgatg gagaagcacc tggtcaaatt ggatggagta atgagttgca acaaaaaacg
     9601 tcatcatttg gtcaatcgac aacagtaaca cctgttcaaa tgttacaagc gcaatcagcg
     9661 ttctttaatg atggtaatat gttaaaacca tggtttgtga atagcgttga aaatcctgtt
     9721 agtaaaagac aattttataa agggcaaaaa caaatcgcag gcaaaccaat aacaaaagat
     9781 actgctgaaa aagttgaaaa gcaattggat ttagttgtga atagtaagaa gagtcacgct
     9841 gcaaactatc gtattgatgg ttatgaggtc gaaggtaaga ctggtacagc acaagtcgct
     9901 gcacctaatg gtggtggata cgttaaaggt ccaaacccat attttgtaag ttttatgggt
     9961 gacgcgccga agaaaaatcc taaagttatt gtatacgctg gtatgagctt ggcacaaaaa
    10021 aatgaccaag aagcttatga attaggtgtt agtaaagcgt ttaaaccaat aatggaaaat
    10081 actttgaaat atttaaatgt aggtaaatca aaagatgaca catctaatgc agagtatagt
    10141 aaagtgccag atgttgaagg tcaagacaaa caaaaagcta ttgataatgt gagtgcaaaa
    10201 tcattagaac cagttactat tggttctggc acacaaataa aagcacaatc tataaaagca
    10261 gggaataaag tcttacctca tagtaaagta ctgttattaa cagatggaga cttaactatg
    10321 cctgacatgt caggatggac gaaagaagat gtcattgctt ttgaaaacct aacaaatatt
    10381 aaagtaaatt taaaaggtag cggttttgtg tcccaccaat caattagtaa gggacaaaaa
    10441 cttactgaaa aagataaaat agacgtagaa ttttcatcag agaatgtaga cagcaattcg
    10501 acgaataatt ctgattcaaa ttcagatgat aagaagaaat ctgacagtaa aactgacaag
    10561 gataagtcgg actaacaaga atggctacat taaattagga tgtattgtca ctatttgttt
    10621 ttactaaagt aatgctgtga tagaaaacaa catcttcatt taatctttat aaataagtgt
    10681 tgatagagaa ttttaaggta gacgaggtca gattaacttg tttgttatag gcaaactttt
    10741 tatactggcc tcttatgccg taaaaaagaa aaattataaa ttttgattac aactttgttt
    10801 atttaattga aaaattgata atatattagt ggttaaaaaa gtctggatta agaagaggag
    10861 atagttatga tttttgtata tgcgttatta gcgctagtga ttacatttgt tttggtacct
    10921 gttttaatac ctacattaaa aaggatgaaa tttggtcaaa gtattcgaga agaaggtcca
    10981 caaagccata tgaagaagac tggtacacca acgatgggtg gactaacatt tctattaagt
    11041 attgtgataa cgtctttggt ggctattata tttgtagatc aagctaatcc aatcatactg
    11101 ttattatttg tgacgattgg ttttgggtta attggtttta tagatgatta tattattgtt
    11161 gttaaaaaga ataaccaagg tttaacaagt aaacagaagt ttttggcgca aattggtatt
    11221 gcgattatat tctttgtttt aagtaatgtg tttcatttgg tgaatttttc tacgagcata
    11281 catattccat ttacgaatgt agcaatccca ctatcatttg catatgttat tttcattgtt
    11341 ttttggcaag taggtttttc taatgcagta aatttaacag atggtttaga tggattagca
    11401 actggactgt caattatcgg atttacaatg tatgccatca tgagctttgt gttaggagaa
    11461 acggcaattg gtattttctg tatcattatg ttgtttgcac ttttaggatt tttaccatat
    11521 aacattaacc ctgctaaagt gtttatggga gatacaggta gcttagcttt aggtggtata
    11581 tttgctacca tttcaatcat gcttaatcag gaattatcat taatttttat aggtttagta
    11641 ttcgtaattg aaacattatc tgttatgtta caagtcgcta gctttaaatt gactggaaag
    11701 cgtatattta aaatgagtcc gattcatcat cattttgaat tgataggatg gagcgaatgg
    11761 aaagtagtta cagtattttg ggctgttggt ctgatttcag gtttaatcgg tttatggatt
    11821 ggagtgcatt aagatgctta attatacagg gttagaaaat aaaaatgtat tagttgtcgg
    11881 tttggcaaaa agtggttatg aagcagctaa attattaagt aaattaggtg cgaatgtaac
    11941 tgtcaatgat ggaaaagact tatcacaaga tgctcatgca aaagatttag aatctatggg
    12001 catttctgtt gtaagtggaa gtcatccatt aacgttgctt gataataatc caataattgt
    12061 taaaaatcct ggaatacctt atacagtatc tattattgat gaagcagtga aacgaggttt
    12121 gaaaatttta acagaagttg agttaagtta tctaatctct gaagcaccaa tcatagctgt
    12181 aacgggtaca aatggtaaaa cgacagttac ttctctaatt ggagatatgt ttaaaaaaag
    12241 tcgcttaact ggaagattat ccggcaatat tggttatgtt gcatctaaag tagcacaaga
    12301 agtaaagcct acagattatt tagttacaga gttgtcgtca ttccagttac ttggaatcga
    12361 aaagtataaa ccacacattg ctataattac taacatttat tcggcgcatc tagattacca
    12421 tgaaaattta gaaaactatc aaaatgctaa aaagcaaata tataaaaatc aaacggaaga
    12481 ggattatttg atttgtaatt atcatcaaag acaagtgata gagtcggaag aattaaaagc
    12541 taagacattg tatttctcaa ctcaacaaga agttgatggt atttatatta aagatggttt
    12601 tatcgtttat aaaggtgttc gtattattaa cactgaagat ctagtattgc ctggtgaaca
    12661 taatttagaa aatatattag cagctgtgct tgcttgtatt ttagctggtg tacctattaa
    12721 agcaattatt gatagtttaa ctacattttc aggaatagag catagattgc aatatgttgg
    12781 tactaataga actaataaat attataatga ttccaaagca acaaacacgc tagcaacaca
    12841 gtttgcctta aattcattta atcaaccaat catttggtta tgtggtggtt tggatcgagg
    12901 gaatgaattt gacgaactca ttccttatat ggaaaatgtt cgcgcgatgg ttgtattcgg
    12961 acaaacgaaa gctaagtttg ctaaactagg taatagtcaa gggaaatcgg tcattgaagc
    13021 gaacaatgtc gaagacgctg ttgataaagt acaagatatt atagaaccaa atgatgttgt
    13081 attattgtca cctgcttgtg cgagttggga tcaatatagt acttttgaag agcgtggaga
    13141 gaaatttatt gaaagattcc gtgcccattt accatcttat taaagggtgt gagtattgat
    13201 ggatgataaa acgaagaacg atcaacaaga atcaaatgaa gataaagatg aattagaatt
    13261 atttacgagg aatacatcta agaaaagacg gcaaagaaaa agatcaaagg ctacacattt
    13321 ttctaatcaa aataaagatg atacatctca acaagctgat tttgatgaag aaatttactt
    13381 gataaataaa gacttcaaaa aagaagaaag caatgataaa aataatgatt ctgcttctag
    13441 tcatgcgaat gataataata tcgatgattc tacagactct aatattgaaa atgaggatta
    13501 tagatataat caagaaattg acgaccaaaa tgaatcgaat gtaatttcag tcgacaacga
    13561 acaacctcaa tcagctccta aagaacaaaa tagcgactcg attgatgagg aaacagtaac
    13621 gaaaaaagaa cgaaaaagta aagtaacaca attaaagcca ttaacacttg aagaaaagcg
    13681 gaagttaaga cgtaagcgac aaaagcgaat ccaatacagt gttattacaa tattggtatt
    13741 gttgattgct gttatattaa tttacatgtt ttcaccactt agtaaaattg cgcatgtaaa
    13801 tataaatgga aataatcacg ttagtacttc aaagataaac aaagttttag gtgttaaaaa
    13861 tgattcaagg atgtatacgt ttagtaaaaa aaatgctatt aatgatctcg aagagaatcc
    13921 attaatcaaa agtgttgaga tacacaagca attaccaaac acattaaacg tagatatcac
    13981 agaaaatgaa attattgctt tagtgaaata taaaggtaaa tatttacctt tattagaaaa
    14041 tggtaaattg cttaaaggtt caaatgatgt caaaattaat gatgcacctg tcatggatgg
    14101 tttcaaaggt acaaaagaag atgatatgat taaggcgtta tctgaaatga cacctgaagt
    14161 tagacgatat attgccgaag tgacatacgc cccaagtaaa aacaaacaaa gcagaattga
    14221 attgtttacg acagatggac ttcaagtaat cggtgatatt tcgacgatat ctaagaaaat
    14281 gaaatattat ccgcagatgt cacaatcatt atcaagggat agttcgggta aactaaaaac
    14341 acgaggctat attgatttat cagtcggtgc ttcatttatc ccataccgtg gaaacacgtc
    14401 tagtcaatca gaaagcgata aaaatgtgac taaatcatct caagaggaaa atcaagcaaa
    14461 agaagaatta caaagcgttt taaacaaaat taacaaacaa tcaagtaaga ataattaaaa
    14521 aattttgata ttgtctatgt ttatagttca caagccattc aacgtattgt aaactaagga
    14581 tagtgtattt ttttaatagt aatttgtcag gaggtgccta tctatggaag aacattacta
    14641 cgtaagtatt gatattggat catcaagcgt aaaaacaata gtaggcgaga aatttcacaa
    14701 tggtataaat gtgataggta caggacaaac ctacacgagc ggtataaaaa atggtttaat
    14761 tgatgatttt gatattgcgc gacaagcaat caaagacaca attaaaaagg catcaatcgc
    14821 ttcgggtgtt gatattaaag aagttttcct gaaattacct atcattggaa cggaagttta
    14881 tgatgaatca aatgaaatcg acttttatga ggatacagaa atcaacggtt cacatatcga
    14941 aaaagtatta gaaggtatta gagaaaaaaa tgatgtgcaa gaaacagaag taattaatgt
    15001 gttcccgatt cgttttatag tcgataaaga aaatgaggtt tcagacccta aagaattaat
    15061 tgccagacat tcattaaagg ttgaagcagg cgtaattgct attcaaaaat cgattttaat
    15121 taatatgatt aaatgcgtag aagcatgtgg tgttgatgta ttagatgttt actctgatgc
    15181 atataactat ggttcaatcc taacagctac tgaaaaagag ttaggtgcat gtgtcattga
    15241 tattggtgaa gacgttacgc aagttgcttt ttatgaacgc ggtgaattag tagatgctga
    15301 ttctatcgaa atggcagggc gtgatattac agacgatatt gcacaaggat taaacacttc
    15361 ttatgaaact gctgaaaaag ttaaacacca atatggtcat gcattctatg attctgcttc
    15421 agatcaagat atcttcactg ttgaacaggt tgatagtgat gaaacagtac agtatactca
    15481 aaaagatttg agtgacttta ttgaagcgcg tgtagaagaa atattcttcg aagtatttga
    15541 tgttttacaa gatttaggat taacaaaagt aaatggtggg tttattgtaa ctggtggatc
    15601 tgcaaactta cttggcgtaa aagaattatt atcagatatg gtaagtgaaa aagttagaat
    15661 tcacacgcca tcacaaatgg gaattagaaa acctgaattt tcttcagcaa tttctacaat
    15721 ttctagtagt atcgcttttg atgagttatt agattatgtt acaattaatt atcatgataa
    15781 tgaagaaact gaagaagatg ttattgatgt gaaagacaaa gataacgaat ctaaattagg
    15841 cggatttgat tggtttaaac gtaaaacaaa caaaaaagat actcatgaaa atgaagtaga
    15901 gtcaacagat gaagaaattt atcaatcaga agataatcat caggaacata aacagaatca
    15961 tgaacatgtt caagacaaag ataaagataa agaagaaagt aaattcaaaa aactaatgaa
    16021 atctctattt gaatgattat tggccaataa aactaggagg aaatttaaat gttagaattt
    16081 gaacaaggat ttaatcattt agcgacttta aaggtcattg gtgtaggtgg tggcggtaac
    16141 aacgccgtaa accgaatgat tgaccacgga atgaataatg ttgaatttat cgctatcaac
    16201 acagacggtc aagctttaaa cttatctaaa gctgaatcta aaatccaaat cggtgaaaaa
    16261 ttaacacgtg gtttaggagc aggagctaat cctgaaatcg gtaaaaaagc tgcagaggaa
    16321 tctcgtgaac aaattgaaga tgcaatccaa ggtgcagaca tggtatttgt tacttctggt
    16381 atgggtggcg gaactggtac tggtgcagca ccagtcgttg ctaaaattgc aaaagaaatg
    16441 ggcgcattaa ctgttggtgt tgtaactcgt ccatttagtt ttgaaggacg taaacgtcaa
    16501 actcaagctg ctgctggagt agaagctatg aaagctgcag tagatacatt aatcgttata
    16561 ccaaatgacc gtttattaga tatcgttgac aaatctacgc caatgatgga agcatttaaa
    16621 gaagctgaca acgtgttacg ccaaggtgta caaggtatct cagacttaat cgctgtttct
    16681 ggtgaagtaa acttagactt tgcagacgtt aagacaatta tgtctaacca aggttctgca
    16741 ttaatgggta ttggtgtttc ttctggtgaa aatagagcgg tagaagctgc taaaaaagca
    16801 atctcttctc cattacttga aacatctatc gttggtgcac aaggtgtgct tatgaatatt
    16861 actggtggcg agtcattgtc attatttgaa gcacaagagg ctgctgatat tgtccaagat
    16921 gctgcagatg aagacgttaa tatgattttc ggtacagtta ttaatcctga attacaagat
    16981 gagattgttg taacagttat tgcaactggt tttgatgaca aaccaacatc acatggtcgt
    17041 aaatctggta gcactggatt cggaacaagc gtaaatactt ctagcaatgc aacttctaaa
    17101 gatgaatcat tcacttcaaa ttcatcaaat gcacaagcaa ctgatagtgt aagtgaaaga
    17161 acacatacaa ctaaagaaga tgatattcct agcttcatta gaaatagaga agaaagacgt
    17221 tcaagaagaa caagacgtta atcggttaat atatatacac aaataattca acacaaatca
    17281 tcagataaca tatctgatga tttttttact aatttttaga acatgtagaa ggacatttaa
    17341 gtttttcaaa gttattaaaa gtgtttaagt atcgtgtgaa aattaagtca aaaattattt
    17401 gcgcaacatt ttaactttaa acataaatgt tatattatat aattattaac tttgtacagt
    17461 tagacgaaga taatttaaat gaaatgatgg tgacgatcga gtgaatgata attttaaaaa
    17521 gcaaccgcat catttaatat atgaagagtt attacaacaa ggtattactc taggtattac
    17581 aactagagga gatggtttaa gtgactatcc taaaaatgct tttaatatgg cgagatatat
    17641 tgatgatcgc ccatataata ttactcaaca tcaattgcaa ttagctgaag aaattgcgtt
    17701 tgatagaaaa aattgggtgt ttcccattca aacacatgaa aataaagtcg cttgtattac
    17761 aaaggatgat ataggcacaa atatagacac tttaactgat gcgcttcatg gtattgatgc
    17821 gatgtacaca tatgatagta atgtcttatt aacgatgtgt tatgcagact gtgtaccagt
    17881 atatttttat agtacaaaac atcattttat tgcattggcg catgcaggtt ggcgtggtac
    17941 ctatactgaa attgtaaaag aagtgctaaa acatgtgaac tttgatttga aagacttaca
    18001 tgtcgttatt ggaccatcta catcatcaag ttatgaaatt aatgatgata ttaaaaataa
    18061 atttgaaaca ttgccaattg atagtgccaa ctatattgaa actagaggac gagatcgtca
    18121 tggtattgat ttgaaaaaag ccaatgctgc attattaatt tattatggtg ttcctaaaga
    18181 aaatatttat acgacagcgt atgctacatc tgaacattta gaattatttt tctcttatcg
    18241 attagaaaaa ggtcaaacag gacgcatgtt agcattcatt ggtcaacagt aaacaaggag
    18301 gagatatgtt tgcgtgtgaa agataattta caacaaatct caacacaaat taatgacaaa
    18361 agtgaaaaaa ataatttttc aacaaaacca aacgtgattg cagttacaaa atatgttaca
    18421 atagagcgag ctaaagaagc gtatgaggct ggaataagac attttggtga gaatagattg
    18481 aaaggctttt ttcaaaagaa agaagcatta ccatcagatg cggtgatcca ttttatagga
    18541 tcattacaat ctcgaaaagt taaggacgtt ataaacgacg tagattattt ccatgcttta
    18601 gatcgattga gcttagccaa agaaattaac aaacgtgcag aacataaaat taaatgtttc
    18661 ttgcaagtga acgtttcggg agaagcttct aaacatggta ttgctttaga agatgttgat
    18721 cagtttatag atgatcttaa aaaatatgac aaaatcgaaa ttgtaggttt aatgacgatg
    18781 gcaccattga cagatgatga agcatatatt agatcgttat ttaaacagtt acgtttgaaa
    18841 aaagaagaaa tacaacgact caatttagaa tatgcgcctt gtgatgaatt atcaatggga
    18901 atgagtaatg actatcttat tgcagttgaa gaaggtgcga cgtttgttag aattgggact
    18961 aaacttgtag gagaagagga gtgagccact tggctttaaa agatttattt agtggatttt
    19021 ttgtaataga tgatgaagag gaagtagaag tacctgacaa acaacaacag gtaaatgaag
    19081 cgccagcaaa agagcagtca caacaaacaa caaaacaaaa cgcaatcaaa tcagtccctc
    19141 aaaaatctgc atcaagatat acaacaacgt cagaagaaag gaataaccgt atgtctaatt
    19201 attcaaaaaa taattcacgt aatgttgtaa ctatgaacaa tgctacacca aacaatgcat
    19261 cacaagaaag ttcaaaaatg tgtttattcg aaccacgtgt tttttcagat acacaagata
    19321 ttgctgatga gcttaaaaac cgccgtgcga cacttgtcaa tttacaacgt attgataaag
    19381 tatcagcgaa aagaattatt gattttttaa gcggtactgt ttatgcaatc ggtggagata
    19441 tccaacgtgt aggtactgat attttcttat gtacgcctga taatgtggaa gtagctggaa
    19501 gcattacaga ccatattgaa aatatggaac attcattcga ctaaggagtt aacatatgga
    19561 tataaatgtg ctagctacaa tatttaaatt catccttttt gttgttgaaa tttattattt
    19621 cggcatgatt atatatttct ttacatcttg ggtaccaagt attagagaaa ctaaggtagg
    19681 ttatttttta gcgaaaatat atgaaccttt cttacaacca tttagaaaag taattccacc
    19741 tattggaatt atcgacatat catcaatcgc tgcaattatc gttttagtat tattccaaaa
    19801 agggttactc caaatcttta attggatttt aattcaatta caataaatta agcgacataa
    19861 cgaggcaagg gtacatgata ctttagcctc gtttttgata tgcatttttc tgaatataag
    19921 ggcaatagat ggtattttat aattttttta aggtagtgat taacatagat atttatcaac
    19981 actttagaca ggaagaatac gaattaattg atcagctaac ggataaatgt gatcaagcgg
    20041 aacagcatta tgcaccagta ttaacgcatt ttttagatcc aagagggcaa tatatattgg
    20101 aagtgatttg tggcagttat gaagatttaa acgtatcttt ttatggtgga cctaatgctg
    20161 aaagaaaaag agcaatcatt tcgccgaact attatgaacc taaagaaagc gactttgaat
    20221 taactttaat ggaaatagat tatcctgaaa aattcgtcac tttaaaacat caacatattt
    20281 tagggacatt aatgtcttta ggtatcgaac gcgaacaagt tggagatata attgtgaatg
    20341 aacgaattca atttgttttg acaagtagat tggaatcatt tattatgtta gaattacaac
    20401 gtattaaagg cgcatcagtt aaactttata ctattccagt aacagatatg atacaatcta
    20461 atgagaattg gaaaaatgaa agtgcaacag ttagttcttt aaggttagat gttgttatta
    20521 aagaaatgat acgtaaatca cgtacgattg cgaaacaact aatcgaaaaa aaacgtgtta
    20581 aagtgaatca cactattgtt gattcagcag attttcaatt acaagcaaat gatttaatat
    20641 ccatccaagg ttttggtaga gcacacatta ctgacttagg tggtaaaact aaaaaagata
    20701 aaacgcacat tacctataga acattattca aatagtaatg atttaaggag gataacaaat
    20761 gccttttaca ccaaatgaaa ttaagaataa agagttttca cgtgtaaaga atggtttaga
    20821 acctactgaa gttgctaatt ttttggagca actaagcact gaaattgaac gtcttaaaga
    20881 agataaaaaa caacttgaaa aagtaatcga agagagagat actaatatta agtcttatca
    20941 agacgtgcat caatctgtaa gtgatgcttt gatacaagct caaaaagctg gtgaagaaac
    21001 taagcaagct gcagagaaac aagctgaagc gattatagct aaggcagaag cgcaagctaa
    21061 tcaaatggtt ggtgacgcgg tagaaaaagc acgccgttta gcattccaga ctgaagatat
    21121 gaaacgtcaa tcaaaagtat ttagatcgcg tttccgtatg ttagttgaag cgcaattaga
    21181 cttattaaaa aacgaagatt gggattactt gttgaattat gatttagacg ctgaacaagt
    21241 gacgcttgaa aatattcatc atttgcatga aaatgattta aagccagatg aagttgcagc
    21301 aaatgcacaa aataatgcat caaatacacc agacaataat caacaatcca atgattcaga
    21361 aacaactaag aagtaagaat taaataaaga cagacgcgta atatacattt aacttttcac
    21421 agcgaattag gtaatggtga gagcctagta aaagcatgta tgttatatca ctggcttttt
    21481 aatatttaaa taatgtaatg agagaactct aagttgagtt aataagggtg gtaccgcgag
    21541 caatcgtccc ttttaattta acttagagtt ttttaaattt ttaaggagtg aaaaaaatgg
    21601 attacaaaga aacgttatta atgcctaaaa cagatttccc aatgcgaggt ggtttaccaa
    21661 acaaggaacc gcaaattcaa gaaaaatggg atgcagaaga tcaataccat aaagcgttag
    21721 aaaaaaataa aggtaacgaa acattcattt tacatgatgg cccaccatac gcgaatggta
    21781 acttacatat gggacatgcc ttgaacaaaa ttttaaaaga ctttattgta cgttataaaa
    21841 ctatgcaagg gttctatgca ccatacgtac caggttggga tacacatggt ttaccaattg
    21901 aacaagcatt aacgaaaaaa ggtgttgacc gaaagaaaat gtcaacagct gaattccgtg
    21961 agaaatgtaa agaatttgct ttagaacaaa ttgaattaca gaaaaaagat tttagacgtt
    22021 taggtgttcg tggtgacttt aatgatccat atattacatt aaaacctgaa tacgaagctg
    22081 cacaaattcg tatttttgga gaaatggcag ataaaggttt aatttataaa ggtaaaaagc
    22141 cagtttattg gtctccttca agtgagtctt cattagcaga agcagaaatt gaatatcacg
    22201 ataaacgttc agcatcaatt tacgttgcat ttgacgttaa agatgacaaa ggtgtcgttg
    22261 atgcagatgc taaatttatt atctggacaa caacgccatg gacaattcca tcaaatgttg
    22321 cgattaccgt tcatcctgaa ttaaaatatg gtcaatacaa tgtaaatggc gaaaaatata
    22381 ttattgcaga agccttgtct gacgctgtag cagaagcact ggattgggat aaagcatcaa
    22441 tcaaattaga aaaagaatac acaggtaaag aattagagta tgttgtagca caacatccat
    22501 tcttagacag agaatcgtta gtgattaatg gtgatcatgt tactacagat gctggtacag
    22561 gttgtgtaca tacagcacca ggtcacgggg aagatgacta tattgttggt caaaaatatg
    22621 aattgccagt aattagtcca atcgatgata aaggtgtatt tactgaagaa ggcggccaat
    22681 ttgaagggat gttctatgat aaagctaata aagccgttac tgatttatta acagaaaaag
    22741 gtgcactatt aaaattagac tttattacac atagctatcc acacgactgg agaacaaaaa
    22801 aacctgtaat cttccgtgct acaccacaat ggtttgcctc aatcagtaaa gtaagacaag
    22861 atattttaga tgcaatcgaa aatacaaact tcaaagtaaa ttggggtaaa acacgtattt
    22921 acaatatggt tcgtgaccgt ggcgaatggg ttatttctcg tcaacgtgtg tggggtgtac
    22981 cgttaccagt attttatgct gaaaatggcg aaattatcat gacgaaagaa acagtgaatc
    23041 atgttgctga tttatttgca gaacacggtt caaatatttg gtttgaaaga gaagcgaaag
    23101 acttactacc agaaggattt acacatccag gcagccctaa cggtacattt actaaagaaa
    23161 cagacattat ggacgtttgg tttgattctg gttcatcaca ccgtggcgtg ttggaaacaa
    23221 gaccggaatt aagtttccca gcggatatgt atttagaagg tagtgaccaa tatcgtggtt
    23281 ggttcaactc ttctatcaca acttcagttg ctacaagagg agtatcacct tataaattct
    23341 tactttctca tggttttgtt atggacggtg aaggtaagaa aatgagtaaa tctttaggta
    23401 atgtgattgt acctgaccaa gtggttaaac aaaaaggtgc tgatattgcg agactttggg
    23461 taagtagtac ggactattta gctgatgtta gaatttctga tgaaatttta aaacaaacat
    23521 ctgatgttta tcgtaaaatc agaaatacat taagatttat gttaggtaac attaacgatt
    23581 tcaatcctga cacagatagc attcctgaat cagagttatt agaagtggat cgttacttgc
    23641 taaatcgttt acgtgaattt actgcaagta cgattaacaa ctatgaaaac tttgactact
    23701 taaatattta tcaagaagtt caaaacttta tcaatgttga gttaagtaat ttctatttgg
    23761 attacggtaa agatatttta tatattgaac aacgtgattc tcatatccgt cgtagtatgc
    23821 aaacagtgtt atatcaaatt ttagttgata tgacgaagtt gttagcacca atcttagtgc
    23881 atacagctga agaagtttgg tctcatacac cacatgttaa agaagaaagt gttcacttag
    23941 cagacatgcc taaagttgta gaagtagatc aagctttatt ggataaatgg cgtacattta
    24001 tgaatttacg tgatgatgtg aaccgtgcat tagaaactgc tcgtaatgaa aaagttattg
    24061 gtaaatcatt agaagctaaa gttacgattg ctagtaacga taaatttaat gcatctgaat
    24121 tcttaacttc atttgatgca ttacatcaat tatttatcgt gtcacaagtt aaagttgtag
    24181 ataagttaga cgatcaggca acagcttatg aacatggtga tattgtcatc gaacatgcag
    24241 atggtgaaaa atgtgaaaga tgttggaact attcagagga tcttggtgct gttgatgaat
    24301 tgacgcatct atgtccacga tgccaacaag ttgtaaaatc acttgtataa ttgaaattgt
    24361 ataaagtact catacagatg atataaatta aagctctctt cataatcatg ttgtagtttt
    24421 tgttgacatg atgaagagag tttttttgtg aataaaaaaa tgaccaagtt accggtcata
    24481 tatgtaaaaa atgtgcgatt tactaaaata aaaattattc aggaatggta caaattctct
    24541 gaggcatata aatgcgttat agttgctatt ctcaattatg ttcgcgataa ttttaagtaa
    24601 aagtaagcac agatattgaa tttgatagga gttaattgaa tgtatcataa cagtaacgca
    24661 aactttgtca atggtatcac tttaaatgtg agagataaga atgaattaaa gccattttat
    24721 gaggacatat taggattaaa tattataaat gagacattaa catcgataca atatgaagta
    24781 ggtcaaaata atcatgtcat tacacttgtt gaattacaaa atggacgtga acctttaatg
    24841 tccgaagcgg gactgtttca tatcgcaatt aaactacctc aaattagtga tttagctaat
    24901 ttactaattc atttaagcga atatgatatt ccagttaacg gaggtataca gcctgcttcg
    24961 ttatcattat tttttgaaga cccggaagga aacggtttta aattttatgt tgataaagac
    25021 gaagcgcaat ggacgaggca aaataattta gtaaaaattg atattagacc attaaatgta
    25081 ccgagattag tgagtcatgc aacaaaattg ttatggttag gtattccaga tgacgctatt
    25141 ataggtgcat tgcatattaa gacaattcat ttatcagagg taaaagagta ctacctcgat
    25201 tattttggat tagagcaatc ggcatatatg gatgattatt caatattttt agcatcgaat
    25261 ggctattatc aacatttggc catgaatgat tgggtatcag caacgaaacg tgtagaaaat
    25321 tttgatacgt atggattagc aattgttgac tttcattatc ctgaaacaac acatttaaat
    25381 ttacaaggtc cggatggtat ctattatcgc tttaatcata tcgaagttga agattagtat
    25441 atactttgaa tggacgaacc atataatgaa tcgtttttaa tgatcttttt atacaagtta
    25501 tgaaggaggc tgggacatta agttcttagg caatg
//
LOCUS       DALAAR010000038        25076 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000038 DALAAR010000000
VERSION     DALAAR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.040"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(122..1108)
                     /locus_tag="O2458_001568"
     CDS             complement(122..1108)
                     /locus_tag="O2458_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ3887433.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            1636..2394
                     /gene="map"
                     /locus_tag="O2458_001569"
     CDS             1636..2394
                     /gene="map"
                     /locus_tag="O2458_001569"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCZ3887434.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            2611..2997
                     /locus_tag="O2458_001570"
     CDS             2611..2997
                     /locus_tag="O2458_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887435.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3012..3713
                     /locus_tag="O2458_001571"
     CDS             3012..3713
                     /locus_tag="O2458_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCZ3887436.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            3710..4753
                     /locus_tag="O2458_001572"
     CDS             3710..4753
                     /locus_tag="O2458_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ3887437.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            4743..5372
                     /gene="vraR"
                     /locus_tag="O2458_001573"
     CDS             4743..5372
                     /gene="vraR"
                     /locus_tag="O2458_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCZ3887438.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5488..6705)
                     /locus_tag="O2458_001574"
     CDS             complement(5488..6705)
                     /locus_tag="O2458_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCZ3887439.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(6983..7258)
                     /locus_tag="O2458_001575"
     CDS             complement(6983..7258)
                     /locus_tag="O2458_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ3887440.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(7265..7729)
                     /locus_tag="O2458_001576"
     CDS             complement(7265..7729)
                     /locus_tag="O2458_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCZ3887441.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            7876..8076
                     /locus_tag="O2458_001577"
     CDS             7876..8076
                     /locus_tag="O2458_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCZ3887442.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            8079..9335
                     /locus_tag="O2458_001578"
     CDS             8079..9335
                     /locus_tag="O2458_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCZ3887443.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            9425..9955
                     /locus_tag="O2458_001579"
     CDS             9425..9955
                     /locus_tag="O2458_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ3887444.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(10215..11360)
                     /gene="yfkAB"
                     /locus_tag="O2458_001580"
     CDS             complement(10215..11360)
                     /gene="yfkAB"
                     /locus_tag="O2458_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCZ3887445.1"
                     /translation="MRNNKPKISIQNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNMRC
                     SHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPLLK
                     YAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQPPL
                     KAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEIHP
                     MYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLSRL
                     RNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLSSD
                     LAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(11405..12451)
                     /locus_tag="O2458_001581"
     CDS             complement(11405..12451)
                     /locus_tag="O2458_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ3887446.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLAES
                     EFIQNVKYGYSDNQPFEYIFFDRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKIT
                     TTQCTAFRNYLLNDAGLSVDYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKP
                     QAKFITREEFDEKVEQITNDTSRQLTRLLFYSGLRIGEALALQWKDYDKIKGEIDVNK
                     KINLSNREIEYNLKKESSKGIIPVPKLIREMLKNMYNESSKRYKYFDENYFIFGGLEP
                     IRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSH
                     LYTDKKHQAMSIFD"
     gene            12564..12743
                     /locus_tag="O2458_001582"
     CDS             12564..12743
                     /locus_tag="O2458_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCZ3887447.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IKSEYKDTYLFRLIN"
     gene            complement(12723..13655)
                     /locus_tag="O2458_001583"
     CDS             complement(12723..13655)
                     /locus_tag="O2458_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887448.1"
                     /translation="MEENKTLKEYLRKFLEGYKCVVENRYNYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNIDIKYLSKHFTSYIYDSKKYVEE
                     QSGLLDFNNYIYYTSIYYGKYIGTVILQNNLDLFFNYGKRLANDHYNTLISKSKERLI
                     NKAHDEIQPFNHLDLNSMKEIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFL
                     IKLKKVNKIKHKDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAV
                     AKGDCDFLLNTLKDIVDENEKILREYTKSINKTE"
     gene            complement(13839..14300)
                     /locus_tag="O2458_001584"
     CDS             complement(13839..14300)
                     /locus_tag="O2458_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ3887449.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVR
                     NLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            complement(14313..14627)
                     /locus_tag="O2458_001585"
     CDS             complement(14313..14627)
                     /locus_tag="O2458_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887450.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSK
                     RK"
     gene            14779..15009
                     /locus_tag="O2458_001586"
     CDS             14779..15009
                     /locus_tag="O2458_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3887451.1"
                     /translation="MPIDTKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFLTKTYAKRKF"
     gene            15028..15207
                     /locus_tag="O2458_001587"
     CDS             15028..15207
                     /locus_tag="O2458_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887452.1"
                     /translation="MEQTIKQFLEFRKQFTPAQWHEINRIIDGQFSKKAAELQLDDQD
                     VEVIKNIITQQKIMK"
     gene            complement(15308..15790)
                     /locus_tag="O2458_001588"
     CDS             complement(15308..15790)
                     /locus_tag="O2458_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887453.1"
                     /translation="MEFIQSTLFSNVVAFLALGLSAYSIFYTRSQNKFSFVISDLNFY
                     YENNFVELNFVVANDSSRTHTLEELIFLDKNKNVLTPINVVLESDEYSSLGIYNPSYL
                     HAPIDKQLDKPEVMIANSSSEFLYKFELEPAFIKIVSNQRINKLKKYKLISTDSYEHN
                     "
     gene            16236..16421
                     /locus_tag="O2458_001589"
     CDS             16236..16421
                     /locus_tag="O2458_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887454.1"
                     /translation="MLNLKELREEKGITRYQLAKLTELQNSTIRSIETEVKNPGFLTV
                     KKICDALQVDIANVKEK"
     gene            16423..17163
                     /locus_tag="O2458_001590"
     CDS             16423..17163
                     /locus_tag="O2458_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCZ3887455.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEK"
     gene            17188..17403
                     /locus_tag="O2458_001591"
     CDS             17188..17403
                     /locus_tag="O2458_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001796056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887456.1"
                     /translation="MNTLYKTTLLITMAVVTWKVWKIERNTRKPVINRNDFSKESTAE
                     TIERHSDPDSGIKLLKAFSDFTKENLT"
     gene            complement(17359..17604)
                     /locus_tag="O2458_001592"
     CDS             complement(17359..17604)
                     /locus_tag="O2458_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887457.1"
                     /translation="MSYSEYEQLYYKIVNEADELYGGQSEHFKKNLQKLTENADEGVS
                     SEKIYSTALHESLEYQRNFIFLELGKVLFSEVGKCLK"
     gene            17675..17896
                     /locus_tag="O2458_001593"
     CDS             17675..17896
                     /locus_tag="O2458_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887458.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDERIMEELKKENE"
     gene            17889..18050
                     /locus_tag="O2458_001594"
     CDS             17889..18050
                     /locus_tag="O2458_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCZ3887459.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            18144..18404
                     /locus_tag="O2458_001595"
     CDS             18144..18404
                     /locus_tag="O2458_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ3887460.1"
                     /translation="MYYEIGEIIRKNIHVNGFNFNLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            18414..18635
                     /locus_tag="O2458_001596"
     CDS             18414..18635
                     /locus_tag="O2458_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HCZ3887461.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDISYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            18628..19407
                     /locus_tag="O2458_001597"
     CDS             18628..19407
                     /locus_tag="O2458_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ3887462.1"
                     /translation="MTEQTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTF
                     LTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVV
                     VIETIQKLRDITMDDIMDGKSKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISG
                     HEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKTYQYVL
                     NAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN"
     gene            19437..19991
                     /locus_tag="O2458_001598"
     CDS             19437..19991
                     /locus_tag="O2458_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ3887463.1"
                     /translation="MKITGRTQYIQETNQEAFMKGGDFLGAGEFTVKVANVEFNDREN
                     RYFTIVFENNEGKQYKHNQFVPPFQQDYQEKQYIELLSRLGIKLNLPDLTFDTDQLIN
                     KIGTIVLKNKFNEEQGKYFVRLSYVKVWNKDDEVVNKPEPKTDEMKQKEQQANGKQTP
                     MSQQSNPFANANGPIEINDDDLPF"
     gene            20004..20669
                     /locus_tag="O2458_001599"
     CDS             20004..20669
                     /locus_tag="O2458_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887464.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIEKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GGE"
     gene            20669..21448
                     /locus_tag="O2458_001600"
     CDS             20669..21448
                     /locus_tag="O2458_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCZ3887465.1"
                     /translation="MVKSIFLQDGEEILVDDEDYERVNQHTWHKAFKDNYRMIVNSDK
                     KHLPDFILKKSFQKIKNNDFTRKNLTTEGNKTRWSKAKCNNSSKYKGVSWDKKNNNWY
                     ACIAVDKKTKNLGHFVNEDEAAKAYNNAVNEYWGGVGYLNIIGEDNRLKKRNYKTNIK
                     QLKRGTDKNNLRGINKIKHRYYSKIFYSGNYIALGGYDDLNKARLVYNKCSSYLHGSD
                     AILNDVPMTDELKEFISNWEVPDKIKALKGEDNGRSIVDKT"
     gene            21420..22223
                     /locus_tag="O2458_001601"
     CDS             21420..22223
                     /locus_tag="O2458_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCZ3887466.1"
                     /translation="MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGK
                     YNEQGYIMLSENLPYNEEMLANEFSRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEK
                     HQNIEGLEKIRAQNRLRKQKQRENNRKLLNGHVTSRDSHATEEDKELDKELERDKEKD
                     IDKNLSSNNSATDVTHEQFEEWWKLYNKKKDKKMSFTKFKSCVKKHTFEQIMQGTREY
                     LKTITDKQYQKYPKTFLTNESYMNDYSEEIKETGIDQLERMKYDESYWD"
     gene            22233..23006
                     /locus_tag="O2458_001602"
     CDS             22233..23006
                     /locus_tag="O2458_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ3887467.1"
                     /translation="MKPLFNEKINESLKKYQPIEVILRQNCDKCGHQYDLYKFENGYE
                     YKDGCECEIQRLAYEEYKRNKQKKLDYIFNQSNVNPSLRDATVNNYKPQNEKQVKAKQ
                     TAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDRIK
                     ATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTN
                     FSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            23000..23158
                     /locus_tag="O2458_001603"
     CDS             23000..23158
                     /locus_tag="O2458_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887468.1"
                     /translation="MVTKEFLKTKLECSDIYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAVVEY"
     gene            23171..23392
                     /locus_tag="O2458_001604"
     CDS             23171..23392
                     /locus_tag="O2458_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HCZ3887469.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKHEDNENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            23402..23806
                     /locus_tag="O2458_001605"
     CDS             23402..23806
                     /locus_tag="O2458_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCZ3887470.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIELQP
                     KFELLPKLDKQRKIEYIADFALYLDDKLIEVIDIKGMPTEVAKLKAKMFRHKYRNIKL
                     NWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            23811..23996
                     /locus_tag="O2458_001606"
     CDS             23811..23996
                     /locus_tag="O2458_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCZ3887471.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDKEKETLADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            23997..24359
                     /locus_tag="O2458_001607"
     CDS             23997..24359
                     /locus_tag="O2458_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887472.1"
                     /translation="MGSVVIINNKPYKFNNFEKELMAKRGINAGIVSKRVRGCWEFSE
                     ALDAPYGMHLKEYREMKQMEKIKQARLERELERERKKEAELRKKKPHLFNVPRKHSRD
                     PYWFDVTYNQMFKKWSEA"
     gene            24359..24613
                     /locus_tag="O2458_001608"
     CDS             24359..24613
                     /locus_tag="O2458_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCZ3887473.1"
                     /translation="MSVISNRKVDMNKTQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWD"
     gene            24613..24861
                     /locus_tag="O2458_001609"
     CDS             24613..24861
                     /locus_tag="O2458_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCZ3887474.1"
                     /translation="MTMIDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQGVKKIFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            24873..25040
                     /locus_tag="O2458_001610"
     CDS             24873..25040
                     /locus_tag="O2458_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887475.1"
                     /translation="MMNAEKHMQMMQMLQNCVIDKYVSHDEYEELIAIDKHGNKMFIK
                     FYPNTEDDTNG"
BASE COUNT         9176 a         3835 c         4401 g         7664 t
ORIGIN      
        1 agagaatttc gaaaagaaat tctacaggca aagcgagttg gggtgggacg acgaaataaa
       61 ttttatgaaa atatcatttc tgtcccactc ccatggtgcc aattagcata aggtacttaa
      121 attaagcata tctgctgtct agcagtcgat aaatcattag aacttcgtat agtatatgac
      181 ttttaatttg attttcacca ctaatttcaa gtgcttttat agtcgaacgt aaagtttcta
      241 cagaatcatc ttctctctta aaagaaccat cataaaatat atctttgatg ctactactaa
      301 tttttagcaa tgccattttt tcgtcacctg aaaagttaac acgagtattt ttaggcaagt
      361 aaatgatatt tgataaatga gtgataaaca aacgattcgt atatgcacgt ttagttaatt
      421 gattgagtaa tttccaatca cattcttttt tcttatgata gcttaattca tcacgttgat
      481 aacttattaa cgtttcaact tgattattta aattgaaaat atttttatat gctttttcgc
      541 ttttatcaga ttgcagtctt gataagataa gttcttggca gcgattgtaa aataatttat
      601 acatcaaggc atctgtctta cttaattttt cttcgacctg accataatac ttaggtggaa
      661 acaccatgaa gttaattaaa cctgatgtca cgagtccaat aattgctgtc aatgttcgag
      721 acaaaaagtt gaatatgtag gcatcatgaa tacctggaat catagctaat gatgttagta
      781 cagcgacatt cgtaccaact tgcaatttga gttttgtaca gaataaaatc gtgaacgttg
      841 cactcaatgc atatgtaaaa ggtgattgat cgccgaataa atatgtaaat aatactgcaa
      901 agcctgcacc aattaccgta gcaggtaatc tacgataacc tttaataagt gatgccttgg
      961 cagttggttc aattgtgact acagctgtta aaatggcata gatgggtgtt aaatctagtg
     1021 ccatacaaaa gacagctgtt aaaaaaatgg caataccagt tttaattgtt ctggcaccaa
     1081 ttaaatgttt ataccattga tcgttcattt tttaacctct aatcatcgta aaatcttagc
     1141 gagcgcttta taataatagt atcgtacatt ggaaaagttc atgtatgtaa aatatttgaa
     1201 ataatcatac ataagcatta ctttgatttt catatacatt aatcaaagtt ctgggctcca
     1261 caatgttata ttgttagtag ttgactgaaa taaaatgcac ttgtaacaag catttttcaa
     1321 ttatagtccg gggccccaac atagagaatt tcaaaaaaga aattctacag gcaatgcagg
     1381 ttggcggggc cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg
     1441 cggggcccca acatagagaa attggatcta caatttctac aggcaatgca agttggggta
     1501 caacgataaa gaaatatttt ttctttatca cactatgtct cactcacttt ccaaaatact
     1561 aaagtaacat ctttagtata tcaaagaatt tttgctataa taagttataa ttatataaaa
     1621 aaggaacggg ataaaatgat tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt
     1681 ggatacatat gcgctaaagt gcgcaataca atgcaagctg caaccaaacc aggtatcact
     1741 acgaaagagc ttgataatat tgcgaaagag ttatttgaag aatacggtgc tatttctgcg
     1801 ccaattcatg atgaaaattt tcctggtcaa acgtgtatta gtgtcaatga agaggtggca
     1861 catgggattc caagtaagcg tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg
     1921 gctttgaaga atggctatta tgcagataca ggcatttcat ttgtcgttgg agaatcagat
     1981 gatccaatga aacaaaaagt atgtgacgta gcaacgatgg catttgagaa tgcaattgca
     2041 aaagtaaaac cgggtactaa gttaagtaac attggtaaag cggtgcataa tacagctaga
     2101 caaaatgatt tgaaagtcat taaaaactta acaggtcatg gtgttggttt atcattacat
     2161 gaagcaccag cacatgtact taattacttt gatccaaaag acaaaacatt attaactgaa
     2221 ggtatggtat tagctattga accgtttatc tcatcaaatg catcatttgt tacagaaggt
     2281 aaaaatgaat gggcttttga aacgagcgat aaaagttttg ttgctcaaat tgagcatacg
     2341 gttatcgtga ctaaggatgg tccgatttta acgacaaaga ttgaagaaga atagttcaac
     2401 atatactaag actaaagtat gaacatcatt tagttccgga gcctattcat attggtttcg
     2461 gaactgtttt ataataatta agaacacaat caatgcgtca tttcaaaaat atgttgtaac
     2521 aaagtagttt ttaagcaaac atatcatcga catcaacgaa gatacatagc gcatttggta
     2581 ttttaaaact tattataaaa ggtgatagtt atgaactatg ttgaacgtta tattgaacag
     2641 tttttgagag caacagtaag aaataatatc aagcactacc ttttaatgct agatgaaaaa
     2701 atgaaaaatt tagatgatta tatgcgttat ttaattacta aaaaagaaca acttagcaag
     2761 ttaattgaca gtctaatgct aacattagaa aataaatata ttgatattgc tgaagcattt
     2821 caaattcaat gtgcaagaga aatcaataat caagaaattg aaaatattaa atcagagttg
     2881 aataaagttg aagcatatta tgcacaaatt gaaactcaaa ttcaacaaac ttcaactgaa
     2941 aaaatagcaa cagaaaaaac atcgtatcta ataaattata tgaacgctgt ggcatagaaa
     3001 ggcggcgaaa catgacacac aaatatatat caacgcaaat gttgatcatt tttactgcat
     3061 taatgattat tgccaatttt tactacatat tttttgaaaa aattggcttt ttactcgttc
     3121 tattattggg atgtgtatta gtttatgtag gatatcttta ttttcataaa atacgtggcc
     3181 ttttggcgtt ttggataggc gcgctattaa ttgcattcac attattgtct aataagtata
     3241 caatcatcat cttgttcgtc tttttattat tacttattgt gcgttattta atacacaagt
     3301 ttaaaccaaa aaaagtagtt gcgacggatg aggttatgac ttcaccatct tttattaaac
     3361 aaaagtggtt tggtgagcaa cgtacaccag tttatgtata taagtgggaa gatgtacaaa
     3421 ttcaacatgg aattggcgac ctacatattg acttaacaaa agctgcaaat attaaggaaa
     3481 ataataccat tgttgttaga cacattttag gtaaagtgca ggttatattg ccggttaatt
     3541 acaatattaa tttacatgta gctgcttttt atggaagtac ttacgtgaat gaaaaatcat
     3601 ataaagttga aaataacaat attcatattg aagaaatgat gaaaccggat aactatacag
     3661 ttaatatcta cgtatcaacg tttatcggag acgtagaggt gatttatcga tgaaccacta
     3721 cattagaaca attggttcaa tgctcatctt agtatatagc atgctagctg catttctgtt
     3781 catcgataaa gtttttgtaa atatcatcta ttttcaaggt atgttttata cacaaatatt
     3841 cggaatacca gtttttttat ttttaaatct catcatcata ttattgtgta ttattgttgg
     3901 ttcggtactc gcttacaaaa tcaatcagca aaatgattgg attaagacgc aaattgagcg
     3961 ttcaatggaa ggcgaaacag ttggcattaa tgatcaaaat atagaaatat atagtgaaac
     4021 gttagattta taccatacac tcgtaccttt aaatcaagaa ttgcataagt tgcgacttaa
     4081 aactcaaaac ttaaccaatg aaaattataa tattaatgat gtgaaagtta aaaagattat
     4141 tgaagatgaa cgtcaaagac tagcacgaga acttcacgat tctgttagtc agcaactttt
     4201 tgcggcaagt atgatgctat ctgctatcaa agaaacgaag ttagaaccac cattagacca
     4261 acaaattcct attttagaga aaatggttca agattcgcag ttagaaatgc gtgctttgct
     4321 gttacattta agaccgcttg gtttaaaaga caaatcttta ggtgagggta ttaaagattt
     4381 agttattgat ttacaaaaaa aagtgccaat gaaagttgtg catgaaatac aagattttaa
     4441 agtgcctaaa ggtattgaag atcatttgtt cagaattaca caggaagcaa tttcgaatac
     4501 attgcgtcat tcaaacggta caaaagtgac agtagaattg tttaataaag acgattattt
     4561 attgttgaga attcaagata atggtaaagg ttttaatgtt gatgaaaaat tagaacaaag
     4621 ttatggactt aaaaatatgc gtgaaagagc tttggaaatt ggtgcaacgt tccatattgt
     4681 atcattgcca gattcaggta cacgtatcga ggtgaaagca cctttaaata aggaggattc
     4741 gtatgacgat taaagtattg tttgtggatg atcatgaaat ggtacgtata ggaatttcaa
     4801 gttatctatc aacgcaaagt gatattgaag tagttggtga aggcgcttct ggtaaagaag
     4861 caattgccaa agcccatgag ttgaagccag atttaatttt aatggattta cttatggatg
     4921 acatggatgg tgtagaagcg acgactcaga ttaaaaaaga tttaccgcaa attaaagtat
     4981 taatgttaac tagttttatt gaagataaag aggtatatcg tgcattagat gcaggtgtcg
     5041 atagttacat tttaaaaaca acaagtgcaa aagatatcgc cgatgcagtt cgtaaaactt
     5101 ctagaggaga atctgttttt gaaccggaag ttttagtgaa aatgcgtaac cgtatgaaaa
     5161 agcgcgcaga gttatatgaa atgcttacag aacgagaaat ggaaatatta ttattgattg
     5221 cgaaaggtta ctcaaatcaa gaaattgcta gtgcatcgca tattactatt aaaacggtta
     5281 agacacatgt gagtaacatt ttaagtaagt tagaagtgca agatagaaca caagctgtta
     5341 tctatgcatt ccaacataat ttaattcaat agttcatatc gaattaagaa aagttactta
     5401 cgccaatcac aatataacat caaatagaca ccaaaaaaca gaggttttcg caataatcat
     5461 tagcgaaaac ctctgttttg ttggtgttta gtctttttta agcttatcaa cgattttgtc
     5521 ttttatattt ttatcatctt ctggataggt atcactagga tgttcatcaa catgatacgt
     5581 acgttgatgt gttgcattac tatcttcatc atattcgtat ctagtgttct cattataatg
     5641 atttttatta ttgtcatcat gttctaatgc tgcttcttca ggtgttttac ctttaattac
     5701 actacgctga tgaatgattg cattgatttc agcaccgaca attataataa aacttgtgat
     5761 atataaccat agtaacaaaa tgatgatacc cgcgatactg ccatatgttt tagaatagtt
     5821 accaaaattt gaaatatacc aaccaaaacc aaatgaacca gctaaccaaa taattgaagt
     5881 aaatactgca cctggtaata ctgacttaag cttcgtttta acgttaggtg caaccgaata
     5941 taacacgata aatatgataa atataataat gattggtaac acaattctaa taaggttaaa
     6001 aatccatttc acttgttcgt caaatccaag tggaccgaat aggaaatgac taattacaga
     6061 accaagcgtt ggtaatgcta gagcaactac aaacacaacg cccataacta cagtgaagac
     6121 aacacttagt agttttaata cgattccatt acggctatct tctacatcgt aagcaacatt
     6181 gaaagaattc ataattgcag tcattccatt tgaagctgac caaattgcta aaatcaaacc
     6241 gatagataat aagccaccac tggagttttg agttatatca ccaattacac tcttaattag
     6301 agtagatgtt tcagcgggtg cattgcttaa catattagta atttgactct gcttaatatt
     6361 gaaaaatggt aataatgtta ataggaaaag caacataggg aacatagcaa gtacgaaatg
     6421 gtaagtcatt tgtgctgcta gtcctgaggc atcatctttt cctatgcgat agatgagata
     6481 tgataagaaa ttagagtctt tcttatattt tgcaggttta tttaatcttg ataagaagaa
     6541 aacctgatca tcttttttag gtttcttaga ttggaattgt tgaggttcaa tatatgtacg
     6601 atcaacatca attttgggat tacttttatt tttctttttt gactcctctt gcgcttcctt
     6661 aacagaatta aggtattttg aagaagagtg atcctttttc gacatgtgca ctccttaacc
     6721 tttctctatg taatgtttta taaagtaact attagtattt atccaattat aaaaaaacta
     6781 aacaatgata tgctttaaaa atgataatat tatagaaatt aattgattta aatctttcat
     6841 aggctatttt gatttaaaat taaaacaaag gcaatactga aacaatgcag tttggccaat
     6901 cattgtatta tcatttaata atgataacta tgaaaggcgt acaccaaatt gttaagtatt
     6961 gccttataaa aacaaaatca tcttagcgtt gattttttct attaacaaat gtttctttcg
     7021 catcctttaa tgaacgttct aattcaggat tattacgacg aatttcttca acaacatctt
     7081 tccagtataa aacttcgtct ttaatcttgc tgactttaga aggcttacga gtgcggttgc
     7141 cattttttgc atctttaact gattgaacta aagcttgacg tgtagattta tcagctaaac
     7201 taattgcacc accaattacg gcaccaatta aaataccagg aacaaattta ttttccataa
     7261 aaaactaccc ctctttcaaa tttgcatctt ttacgatgta gtctattaaa ttatcacaag
     7321 atgataatac catgtcgtat acaccttcaa aattattcgt gtagtatgga tctggtacat
     7381 cactctcttc catattacta aattctaaca gtttgaacaa ttgtccctta agattaggat
     7441 tgatagattt aatattatca acgttacttt gatccatagc cacaatgtaa tcaaaatcat
     7501 ctgtcgcttc gaataattca ctaatcatgc catcaaatgg aatattgtgt ttgttgagaa
     7561 ttttttgtgt accttcatga ggtggctctc ctaaattcca gctaccagta cctcttgaat
     7621 gtactttaat atcatgaata tttctgtctt taagtctttg tcgcatgatt gcttctgcca
     7681 ttggagaacg acatatattg ccaagacaga caaatgctac atctaccatt ttgattcctc
     7741 caaactatgt agttatatcc ccattttata gcgactttaa acaataagaa agcagattat
     7801 ataaaattct attaaagttt attaaattgt gatactttga taacataact attattagag
     7861 gtgaacattg tggctatgac aaatgaagag aaagtattag ctattagaga gaagttaaat
     7921 attgttaatc aaggattatt agatcctgaa aaatataaaa atgcaaatga agaagaatta
     7981 acagatatat atgattttgt tcaatcaaga gaaagattgt cgccaagtga agtgacagct
     8041 attgctgacg ctttaggaca attgcgacac gaataggagt gggaattttg actaattaca
     8101 aagaaaagtt acaacaatac gctgaactat tagttaaggt tggtatgaat gttcaaccaa
     8161 aacagccagt atttattaga tcatcagtag aaacattaga attaacgcat ttaattgtag
     8221 aagaagcata tcattgtggc gcttcagatg tacgtgttgt atatagcgat ccaaccttaa
     8281 aacgcttaaa atttgaaaat gaatctgtgg aacattttgc taatcatgag ataaaatcat
     8341 atgatgttga agcgagaatg gattacgtta aacgtggcgc tgctaattta gcacttatta
     8401 gtgaagatcc tgatttaatg gatggtatcg atagccaaaa attacaagca tttcaacaac
     8461 aaaacgcacg cgcatttaaa ggatatatgg aaagtgtaca aaaaaatcaa tttccatggg
     8521 tcgtagctgc ttttccatct aaagcttggg cgaagagagt gtatcctgaa ctatctgtag
     8581 aagaagcata tattaagttt attgacgagg tattcgatat tgtgcgaatt gatggtaacg
     8641 atccagttga aaattggcgt caacacatcg ctaatttaag tgtttacgct caaaagttac
     8701 aacaaaaaaa ttaccatgcg ttgcattatg tatctgaagg tactgattta acagtaggtt
     8761 tagcgaaaaa tcatatctgg gaagatgcta caagttatgt taacggcaag gaacaagctt
     8821 ttattgctaa tataccaact gaagaagtat ttacagcacc agatcgaaat agagtagatg
     8881 gatatgttac taataaattg cctttaagtt ataatggcac aattatcgat caatttaaat
     8941 tgatgtttaa agatggagag attattgatt tttcagctgg aaaaggtgaa gcggtattga
     9001 aagatttaat caatactgat gaaggttcaa gaagattagg tgaagtagca ttagtacctg
     9061 atgattcacc gatatcaaat cgtaatacca ttttttacaa tactttattt gatgaaaatg
     9121 cagcatgtca tttagcgatt ggatctgctt acgcctttaa tattcaaggt ggaacggaaa
     9181 tgactgttga ggaaaagatt gcaagcggat taaatgattc aaatgtacat gtcgatttca
     9241 tgattggtag tagtgatttg actatttatg gcatattcga agatggttca aaagaactag
     9301 tatttgaaaa tggaaattgg gcatcaacat tttaataaat gttattttga ggtgctgagt
     9361 aggaaatgaa acatgtattt aaaggtcaaa tacgtgatta aagtatagat tgggagataa
     9421 aataatgaca aatcaggaca gaccaatgaa atctatgtca gaatcaaaat gttataaaaa
     9481 tagacaagtt ttccctcaag atacgaatca ccatcataca atgtttggtg gtacattgat
     9541 ggctaatatt gatgaaattg cagcaatcac agctatgaaa catgctggtg cgcaagtagt
     9601 taccgcatct acagactcag tagatttctt aaagccgatt aaaacagggg acatattaca
     9661 atacgtagcg atggtttcat acgctgggac tagttcaatg gaagtggtcg ttcaaattag
     9721 aattgacgat gtatttaata acaagcatga tttagctgca ttaagttact taacatttgt
     9781 tgcgttagat gatgaaggca aacctaaaca tgtacctggt gtatatccag aagatgatgt
     9841 tgaaaaatgg ttttatgata cagcaccaca acgtgttgaa cgaagaaaag cacgtcgcat
     9901 tgaaagtaaa caaacaattg aatatttagc tcaagtgcaa catattagag actaaaaata
     9961 taaagacaga ttgttaagga tgaattgaac tgttatgttc gaatattcca cctacaatct
    10021 gtctttttga ttatgcacgt ttatgctttt agttaaaatt tacccctttt attatagact
    10081 attagtccta ttaaatgccc tttattattt aagtagagta ctgccagcaa ttaattgttt
    10141 tttattttat aatagcatta aaatatgcgg agactactta tcgataaagt gcttctccgc
    10201 ataattaaga gtttttaaaa ttgtataatt tgtggttgtt tgtgcatatg acgctcatta
    10261 tctttaaaat ccatattcgg atagtacata tttttaacaa gaacatttgg tcctaaacaa
    10321 ctaaactcgg aacaatgaca atttaatgat ttagcaagat cagaggataa ccatttatca
    10381 aatacatctg ttaatttatc tttttgtata ttcgaaattg tacctgtttc atctccgaaa
    10441 tcagttacga ttacattacc tgtaaataca ttgacattta aacgactacg gccatccggg
    10501 tcatttctag tcgttacatt tttagcattt cttaaacgtg atagtaactt ttgatcttca
    10561 tcatccttta agcatggaaa cacaggcaaa gtaccaaata acatccaaat atcttcatct
    10621 ctgaaatcca atatatcatg aattgtcttt ttcatttccg ctagagttaa cacatttaat
    10681 tgacttgcaa agtcagctgg atacataggg tgaatctcgt gtctgctaca tttcatatca
    10741 tgaacgactt cttgatgtat ttttcgtaaa tgtggtagcg tactttgatt gagcattgtt
    10801 tccgcagata caaacattcc ttgttctgat aatgtacgtg cattcgaaat catttgttca
    10861 tataatttta acttagcttt taacggtggt tgcttcttca ttgcgccaaa gccaacattt
    10921 gcgaattcat cagttgttcc ccagttgtgt gagatatgca taacatcgat atattcagca
    10981 atatctaaat aacgatcttg aggcaatgtt aggtttgaat tcatttgtgt atatatacct
    11041 cgatgatgtg catactttaa tagaggttta acaacatttc taatagactt tttagaaaac
    11101 attggttcgc cacctgtaat agacatcgtt cgcagatttg gaatttcatc aagtctacga
    11161 taaattaagt ccatatctaa aggctcgggg tcgacagttt gtaaagtata accaactgca
    11221 cagtggctac aacgcatatt acaaagattt gtagttgtaa attcgatgtt acttaaagtt
    11281 aattggccat gttctttaac atcgttatat gcttcccatg ggtcgttttg aatacttatt
    11341 ttaggcttgt tattacgcat tttataaact ccttaattgt tatttgatac caatttgata
    11401 ccgtttaatc aaatatgctc atagcttgat gttttttatc agtatataaa tgagagtacg
    11461 tttgaattgt ttctgtaatg ttagagtgcc tcattaattc cattaataaa tacatatcta
    11521 caccattatt aattaaatag cttgcgtacg agtgtcttaa atggtgtatt tttagattcg
    11581 ggaatacaga tttaaaatga tacgaatagg taacgtatct aataggttct aaccccccga
    11641 atataaaata gttttcgtca aaatatttat atcttttaga agattcatta tacatgtttt
    11701 taagcatctc tctaattaat tttggtacag gtattatccc tttagaactt tcttttttta
    11761 gattatattc aatttctcta ttacttaaat tgattttctt atttacgtca atttcgcctt
    11821 ttattttatc gtaatctttc cactgcaaag ctaaagcttc tcctattcta agaccagaat
    11881 aaaataacag tctagttagc tgacgagaag tatcatttgt gatttgttct actttttcat
    11941 caaattcttc acgagtgata aatttagctt gtggttttgt tctgggaata ggagttaccg
    12001 ataatgtggg gtcgtataag agcttgtaat gctttttggc gtaattgata actgctttaa
    12061 aacctgccca cacagatcgt gcatagtcaa cagaaagacc tgcatcgttt aacaaataat
    12121 tcctgaaagc agtacattgc gtagtagtga ttttgccaat agggatattt ccgaaccttt
    12181 cttttatgtg agtattatat tctgtagttc gcttttctat tgagcgtgca gaaagatttt
    12241 cattttttaa acgatcaaaa aatatatatt caaagggttg attgtccgag tatccatatt
    12301 taacattttg tataaattcg ctttcagcta gtttggcatc tttcttacgt tcaaacccac
    12361 gcttcatttt tcgtttgtta ttaccgtata catctttata tctaatggaa aaataccatt
    12421 tacctgtatt atcatcctta tatactggca ttttgctttt ccctcctcaa aattggcaaa
    12481 aaataataag ggtaggcggg ctacccgtga ttttagtact aggtactaaa tgtgatataa
    12541 taaaataaaa agtaggtgat gaaatgtgtg taaaatttac tgacgcagaa atagcttata
    12601 taaaagaatc agttgaaaat tatagtagtg aatttgatat ttatgacgat gaacaagaac
    12661 ttaaattaaa aatttatgaa caaattatgt taaaaatcaa atctgaatac aaggatacct
    12721 atttattccg tcttattaat tgatttggta tattctctta atattttttc gttttcatca
    12781 acaatgtctt ttagtgtgtt taaaagaaag tcacaatcac ctttggctac tgcaccagct
    12841 tgtgaatggt tgattatgtt tctcatacta tacgcaattt ctacccgttt tttggttcta
    12901 taatttactt taccttcttt agttaattct cctaataatt ttgtgtacat agttgaatcg
    12961 gtgtctttat gtttgatttt attaactttt tttaatttga ttaaaaacgt ttctatagca
    13021 acagcaaagg ttgctgcagc tggcaaatac aattcccttt tataagcttg taatccttgt
    13081 tctatttgat aagaaaaagt tatatcatca acaatctctt tcatactatt taaatctaag
    13141 tggttgaacg gttgtatttc atcatgtgct ttgtttatca atctttcttt cgacttcgat
    13201 atcaatgtat tgtaatgatc gttagctaat cttttgccat aattaaaaaa taaatctaaa
    13261 ttgttttgta atattacggt cccgatatat tttccgtagt aaatagatgt gtaataaatg
    13321 taattattaa aatctaataa tccggattgt tcttctacat actttttaga atcatatatg
    13381 tatgaagtaa agtgtttaga caaatatttg atatcaatat tacgaaaatt atatatttct
    13441 tttaatttac tgtcatttga gataacgacg atgcaaggtt cttcaaaaaa agattgattt
    13501 agataaaata tcgaaatctt gtaatcgtct tttctcatga atgggaaagc ttctggattg
    13561 ctactaaact gataattgta tctgttttca actacacatt tgtagccttc taaaaattta
    13621 cgcaagtatt cttttaaagt tttattttct tccatccctc atcctcctca cgccacatag
    13681 gcgctattaa tcttccttct ttcttattga aaaaataaaa aagatgattg ggatgcttaa
    13741 cattaatgga aaaaatatga ctattggtaa tgacagtacc gccatatata agaagaattt
    13801 atcaaaatta tattttctca ttttcatttc tcctttgttt atatttcctt atatttaaaa
    13861 actctcaacg gctcaaatgt gatcgaatac tcgccatagt gagttccaat accatatatc
    13921 tttttatatt gttctattgc ttctaatatg tattcttcgc ttaattgtag atactcagac
    13981 aattcataca agttacgtac gccataatta taagcttcta caatttcgcg taatggaaca
    14041 gctgagataa agccgtgtct acgtgcgtaa ttttcgaact tgcgattgtt gaatttcgag
    14101 taatcggcta tatcaccgta tgtaagttta ttatgtgcta attcttcaaa gagaatccct
    14161 gccttttctc tatctgataa accacgcttt atcaaaatta aatctcctaa ccataccccg
    14221 tctaaattat ctggaagcac atcagcctct cttatttcaa tataatcatg ttgtattaaa
    14281 gtttcttcat ataatcccat ctgatacatc ctttacttac gtttgcttct tatataatct
    14341 gcataatcta aaactctttg ccattcgtca tctgtcaatt ctccttcaag atgagctgca
    14401 cgatgttgta cttcatcatc gttttcttca acccacccca ttaaatacgc aggattaaca
    14461 tttaatgcag tagctatact ttctatagta tcgtttttta gatttttgat atttccgctt
    14521 tcataacgtt gtacagtagc ttcagtttta ccaatttttc ttcctagttc ggccaaagtc
    14581 ataccttgtt tttctcttga ttgtttcatt ctttttgaaa agcacatcgt aatacagctc
    14641 cttttacttg atagttctat tataaggaaa actttcggca tttgcaatat ttttctaaaa
    14701 aactttcgta aaatgcttga cctcttttgt aacatcatga taagattact tacgtaatgc
    14761 gaaaggtggt gaaaagaaat gcctatagat actaaacttt tgaaatctaa aatggctttg
    14821 aaagaacata acatcaaaac cctttctgaa gaaattggtg tcaatagaga tactttatct
    14881 aacatgatac acgggagaac aaaaccatcc tacccggtaa taaatggtat ttattttgcg
    14941 ttagaattga cacctcaaga aggaagagat atttttttaa cgaagactta cgcaaaaaga
    15001 aagttttaac ttaaggagga acaacaaatg gaacaaacaa tcaaacaatt tttagaattt
    15061 agaaagcaat tcacacccgc acagtggcac gaaatcaaca gaattattga cggacaattt
    15121 agtaaaaaag ccgccgagct acaactcgac gaccaagatg ttgaggttat taaaaatatt
    15181 attactcaac aaaagattat gaagtaacaa tttgaataaa agttattcaa aaatcacgaa
    15241 aggaagatac aaaatgatgc tgaccaacac actactagca attcactttt tcatgaattt
    15301 agcgatatta attatgctcg taagaatcgg tagagattaa cttatacttt ttaagtttgt
    15361 ttattcgttg attagaaaca atctttataa acgcaggttc taattcgaat ttatataaaa
    15421 actctgatga cgaattagct atcataactt ctggtttatc taattgttta tcgattggag
    15481 catgcaaata acttggatta tatataccga gagatgaata ttcatcagat tccaatacta
    15541 cgttaatagg tgttaaaaca tttttgtttt tatctaaaaa tattaattct tctaaagtat
    15601 gagttctaga cgagtcatta gcgacgacaa aatttaattc tacaaaatta ttttcatagt
    15661 agaaattaag atcgctaata acaaaactga acttattttg agaacgagta taaaaaattg
    15721 agtatgcaga tagacctaaa gctagaaaag ctacaacgtt tgaaaacaaa gtagattgta
    15781 tgaattccat aagaataacc accttaaata tttgataaca acattataca tgaaaggagc
    15841 ataaatatta tgcaagcatt acaaacattt tgtttccaat aaaaaaacac acaccttgtc
    15901 gtagaaggta tgtgttacgg aaattttgtt cggttctaat tactacgact aacagcacaa
    15961 tttttgctgg tatcgtcccc agccctgtat ggtgcttagg ttttccgtca aagtctagcg
    16021 tcctaaaagt tactaccttc tagtacgcat accctagtta acgtctcttg gttgactgtg
    16081 gaacacaaca aacgatgttc taatttagac ttactaacct ataaaaccac aggatgattt
    16141 aaaacctggc ataagcaagg aaatcacctc ccaatgtagt ggggttggat taattatata
    16201 acgaaatatc gttatggaca ataaggagtg gtaagatgct gaacttaaaa gaattgagag
    16261 aagaaaaggg gataacacgc tatcaactag cgaagctaac ggaattacaa aactcgacaa
    16321 ttcgatctat cgaaacagag gttaaaaacc ccggtttcct cacagtaaaa aaaatatgcg
    16381 atgcactaca agttgatatc gctaatgtaa aggagaaata aaatgcaagc attacaaaca
    16441 aaatcgaaca tcggcgaaat gttcaacata caagaaaaag aaaatggaga aatcgcaatc
    16501 agtgcaagag agttatacaa ggctttggaa gtcaaaaaac gttttagcgc ttgggcagaa
    16561 attaacttaa agcattttaa agaaaacaga gattttacaa gtgtacttac aagtacggtt
    16621 gttaataacg gagctgtaag acaactagaa gattatgctt taacacttga tgtagctaaa
    16681 catgttgcga tgatgtcagg tacagaaaag ggttttgatt ttagagagta tttcatccaa
    16741 gttgaaaaag cttggaatag cccagaaatg attatgcaac gtgctttaaa aattgctaac
    16801 aacacaatca atcaattaga aacaaagatt gaacgcgaca aaccaaagat tgtatttgcg
    16861 gacgcagtag ctactactaa gacatcaatt ttagttggag agttagcgaa gatcattaaa
    16921 caaaacggtg taaacatcgg gcaacgcaga ttgtttgagt ggttacgtca aaacggattc
    16981 cttattaaac gcaagggtgt ggattacaac atgcctacac agtattcaat ggaacgtgag
    17041 ttattcgaaa ttaaagaaac atcaattaca cattcggacg gtcacacatc aattagtaag
    17101 acgccaaaag taacaggcaa aggacaacaa tactttgtta ataagttttt aggagaaaaa
    17161 taaaaatctt aataggagga attatcaatg aacacactat acaaaacaac cctcctcatc
    17221 acaatggcag ttgtgacttg gaaggtttgg aagattgaac gaaatacgag aaagcctgta
    17281 atcaatagaa atgattttag taaagagtct acagcagaaa cgattgagcg acacagtgat
    17341 cctgattcag gaataaaact acttaaggca ttttccgact tcactaaaga gaaccttacc
    17401 taattctaag aagatgaagt ttcgttggta ctcaagtgat tcatgtaaag cagtagagta
    17461 aatcttttca ctggaaacac cttcatcagc attctctgta agtttttgaa ggttcttttt
    17521 gaagtgttca ctttgaccac cgtatagttc atcagcttca ttaacaattt tatagtaaag
    17581 ctgttcatat tcactatatg acatattatc cacctccttt cactaggaga taactaaatt
    17641 atacacaaca caaaaataaa aaggaggaat agatatgata aaaaatagtt tgcaagctaa
    17701 agaacttgcg gtaattttat ctgtttctaa atccaaagca ggacaaataa taagagaact
    17761 gaataaagag cttgaagatg aaggatacat tgcgatacga ggcagaatac cagtccaatt
    17821 agctagaaaa aaattccctt atcacgactt atcagacgag agaataatgg aggagttgaa
    17881 aaaagaaaat gagtaacatt tataaaagct acctattagc agtactgtgc ttcacggtct
    17941 tagcgattgt actcatgccg tttctatact tcactacagc atggtcaatt gcgggattcg
    18001 caagtatcgc aacattcata ttctataagg aatactttta tgaagaataa aaaaactgct
    18061 acttgcgcca acaagtaaca gtgacaaacg attaacaaaa ttaattcgtg ttcaatataa
    18121 aacgaaaaac ggaggaagtc aagatgtatt acgaaatagg cgaaatcata cgcaaaaata
    18181 ttcatgttaa cggattcaat tttaatctat tcattttaaa aggtcatatg ggcatatcaa
    18241 tacaagttaa agatatgaac aacgtaccaa ttaaacatgc ttatgtcgta gatgagaatg
    18301 acttagatat ggcatcagac ttatttaacc aagcaataga tgaatggatt gaagagaaca
    18361 cagacgaaca ggacagacta attaacttag tcatgaaatg gtaggaggtt gctatgaagc
    18421 agactgtaac ttatatcatt cgtcataggg atatgccaat ttatataact aacaaaccaa
    18481 ctgataacaa ttcagatatt agttactcca caaatagaaa tagagctagg gagtttaacg
    18541 gtatggaaga agcgagtatc aatatggatt atcacaaagc aatcaagaaa acagtgacag
    18601 aaacaattga gtacgaggag gtagaacatg actgaacaaa ctaatcaaga tgtcgatatt
    18661 ttaacgcaac taggtgtaaa agacatcagc aaacaaaatg caaacaagtt ttataaattt
    18721 gcgatatacg gcaagttcgg tactggtaaa actacgtttt taacaaaaga taacaatgcc
    18781 ttagtactag atataaatga ggacggaaca acggtaacag aagatggggc agttgtgcag
    18841 attaagaatt ataagcattt tagtgcagtg attaaaatgc tgcctaaaat tattgaacaa
    18901 ctaagagaaa acggaaaaca aattgatgtt gtagtgattg aaacaatcca aaagttacgt
    18961 gatatcacta tggacgacat catggacggt aaatcaaaga aaccgacatt taatgattgg
    19021 ggcgagtgtg ctacacgcat tgtaagtatt tatcgttata tttctaaatt acaagaacat
    19081 tatcaatttc atcttgctat aagcggacac gagggcatta acaaagacaa agatgatgag
    19141 ggaagtacta tcaatccaac aatcacgata gaggcacaag accaaataaa aaaagcagtc
    19201 atcagtcaat ctgacgtgtt agcaagaatg acaatagaag aacatgagca agacggcgaa
    19261 aaaacttatc aatatgtact taacgctgaa ccatcaaact tattcgagac aaagataaga
    19321 cactcaagca acatcaaaat taacaacaaa cgtttcatta atccaagtat taacgatgtt
    19381 gtacaagcaa ttagaaatgg taattaaaaa ctaattaaaa ggacggtata aaaattatga
    19441 aaatcactgg tagaacacaa tacattcaag aaactaatca agaggcattc atgaaaggtg
    19501 gggacttttt aggagctgga gaatttacag taaaagttgc aaatgtcgag tttaacgaca
    19561 gagaaaacag atacttcacg attgtttttg aaaacaacga aggtaaacaa tacaaacaca
    19621 accaattcgt cccaccattc caacaagatt atcaagaaaa acaatatatc gagttactta
    19681 gtagattagg aattaaattg aacttaccag atttaacttt tgacacagat caattaatta
    19741 acaaaatcgg aactattgta cttaaaaata aatttaacga ggaacaaggc aagtattttg
    19801 taagactctc atatgtaaaa gtttggaata aagacgatga agtagttaat aaaccagaac
    19861 ctaaaactga tgagatgaaa caaaaagaac agcaagcaaa tgggaaacag acgccaatga
    19921 gtcaacaatc aaacccattc gctaatgcta atggtccaat agaaattaat gatgatgatt
    19981 taccgttcta ggacgtggtt taaatgcaat acattacaag ataccagaaa gacaatgacg
    20041 gcacttattc cgtcgttgct actggtgttg aacttgaaca aagtcacatt gacttgctag
    20101 aaaacggata tccactaaaa gcagaagtag aggttccgga caataaaaag ttatctatag
    20161 aacaacgtaa aaaaatattc gcaatgtgta gagatataga acttcactgg ggcgaaccag
    20221 tggaatcaac tagaaaatta ttacaaacag aattggaaat tgaaaaaggt tatgaggaaa
    20281 tcagtctgcg tgactgttca atgaaagtcg caagggagtt aatagaactg attatagcgt
    20341 ttatgtttca tcatcaaata cctatgagtg tagaaacgag taagttgtta agtgaagata
    20401 aagcgttatt atattgggct acaatcaacc gcaattgtgt aatatgcgga aagcctcacg
    20461 ctgacctagc acattacgaa gcagtaggta gaggcatgaa cagaaacaag atgaatcact
    20521 atgacaaaca tgtattagcg ttatgtcgcg aacatcacaa cgagcaacat gcgattggcg
    20581 ttaagtcgtt tgatgataaa tatcacttgc atgactcgtg gataaaagtt gatgagaggc
    20641 tcaataaaat gttgaaagga ggagaataat ggttaaatcg atatttttac aagatggaga
    20701 agaaatttta gttgatgatg aagattacga gagagttaat cagcatactt ggcataaagc
    20761 ttttaaagat aattacagaa tgattgtgaa tagtgataaa aagcatttac ctgattttat
    20821 tctaaaaaaa agtttccaaa aaataaaaaa caatgatttc acaagaaaaa atctaacaac
    20881 tgaaggtaat aaaacaagat ggagcaaagc gaagtgtaac aattcatcta aatataaagg
    20941 cgtttcatgg gataaaaaaa ataataattg gtatgcatgt atagctgttg ataaaaaaac
    21001 caaaaactta ggtcactttg taaatgaaga tgaagcagca aaagcttaca acaatgcagt
    21061 taatgaatat tggggtggtg ttggttacct taatataatt ggagaagata ataggctgaa
    21121 aaaaagaaac tataaaacaa acataaagca attgaagagg ggaactgata aaaacaattt
    21181 aagaggaata aacaaaataa aacatagata ttattcaaaa atattttatt ctggcaacta
    21241 tatagcgtta ggcggatatg acgatttaaa caaagcgaga ttagtttaca acaaatgttc
    21301 gtcatacctg catggatctg acgcgatcct taacgacgta cctatgacag atgaacttaa
    21361 agaattcata tctaactggg aagtaccgga caaaataaaa gcgctgaaag gagaagacaa
    21421 tgggagaagt atcgtggata aaacttaaag ttggcatgtt tgatgacagc aaaatcaaat
    21481 atatcgaagc tttacccgaa agagatacga tcataaccat ttgggttaag ttgctaactt
    21541 tatcaggaaa gtacaacgaa caaggttaca ttatgttatc tgaaaacttg ccgtataacg
    21601 aagaaatgtt agcaaatgag tttagccgac ctattaactc aataaggtta gcaatacaaa
    21661 cttttgagac gttgggcatg attgaaaaag ttaatggtgt cataaaagtg acaaactggg
    21721 aaaaacacca aaacattgaa ggactcgaga aaatcagggc tcagaacagg ttgaggaaac
    21781 aaaagcaacg agaaaacaac agaaaattgc taaatggtca cgtgacgtca cgtgacagtc
    21841 acgcaacaga agaagataaa gaattagata aagaattaga aagagataaa gaaaaagata
    21901 tagataagaa cttaagttca aataatagcg caactgacgt tacgcatgag caatttgagg
    21961 aatggtggaa actttacaac aagaaaaaag ataagaagat gtctttcact aaattcaaat
    22021 catgcgtaaa gaaacatact tttgagcaaa tcatgcaagg tactcgagag tatttaaaaa
    22081 ctattacaga caaacaatat caaaagtacc ctaaaacgtt tttaactaac gaaagctata
    22141 tgaatgatta tagcgaagag attaaagaaa ctggtataga tcaattggaa cgtatgaagt
    22201 acgacgaaag ttattgggac taggaggatg ttatgaaacc gttattcaac gaaaaaataa
    22261 acgaaagttt aaaaaaatat caaccaatcg aagtaatact aagacagaat tgcgataaat
    22321 gcgggcatca atatgactta tataagtttg aaaatggata tgaatacaaa gacggttgcg
    22381 aatgtgaaat tcaaagattg gcttacgaag aatacaaaag gaataaacaa aagaaacttg
    22441 attatatttt caatcaatca aatgttaatc cgtcattaag agatgcaacg gttaacaact
    22501 ataagccaca aaatgaaaaa caagtaaaag ctaaacaaac agcaatagag tatgtacagg
    22561 gtttctctac aaaagaacca aaatcattaa tattgcaagg ttcatatgga actggtaaaa
    22621 gccacctagc atacgctatc gcaaaagcag tcaaatctaa agggcataca gttgctttta
    22681 tgcacatacc aatgttgatg gatcgtatca aagcgacata caacaaaaat gcagttgaaa
    22741 ctacagacga gctagtcaga ttgctaagtg atattgattt acttgtacta gatgatatgg
    22801 gtgtagaaaa cacagagcac actttaaata aacttttcag cattgttgat aacagagtag
    22861 gtaaaaacaa catctttaca actaacttta gtgataaaga actaaatcaa aatatgaact
    22921 ggcaacgtat caattcaaga atgaaacaca atgcgagaaa agtaagagta atcggagacg
    22981 atttcaggga gcgagatgca tggtaaccaa agaattttta aaaactaaac ttgagtgttc
    23041 agatatttat gctcagaaac tcatagacga ggcacagggc gatgaaaata ggttgtacga
    23101 cctatttatc caaaaacttg cagaacgtca cacacgcccc gctgtcgtcg aatattaagg
    23161 agtgttaaaa atgccgaaag aaaaatatta cttataccga gaagatggca cggaagatat
    23221 taaggtcatc aaacatgaag ataacgagaa tgaagtttat tcgctcacag gagcccattt
    23281 cagcgacgaa aagaaaatta tgactgatag tgacctaaaa cgattcaaag gcgctcacgg
    23341 gcttctatat gagcaagaac taggattaca agcaacgata tttgatattt agaggtgcac
    23401 gatgagtaaa tacaacgcta agaaagttga gtataaaggg attgtatttg acagcaaagt
    23461 agaatgtgaa tattaccaat atttagaaag taatatgaat ggcactaact atgatcgtat
    23521 cgaactacaa ccgaaatttg aattattacc aaaactagat aaacaacgaa agattgaata
    23581 tattgcagac ttcgcgttat atctcgatga caaactgatt gaagttatcg acattaaagg
    23641 tatgccaacc gaagtagcaa aacttaaagc taagatgttc agacacaaat acagaaacat
    23701 aaaactcaat tggatatgta aagcgcctaa gtatacaggt aaaacatgga ttacgtacga
    23761 ggaattaatt aaagcaagac gagaacgcaa aagagaaatg aagtgatcta atgcaacaac
    23821 aagcatatat aaacgcaacg attgatataa gaatacctac cgaagttgaa tatcagcatt
    23881 ttgatgatgt ggataaagaa aaagaaacgc tggcagatta cttatataac aatcctgacg
    23941 aaatactaga gtatgacaat ttaaaaatta gaaatgtaaa tgtagaggtg gaataaatgg
    24001 gcagtgttgt aatcattaat aataaaccat ataaatttaa caattttgaa aaagaactaa
    24061 tggcaaagcg cgggataaac gctggaattg tttctaaacg tgttagaggt tgttgggagt
    24121 tttcagaagc tttagacgcg ccttatggca tgcacctaaa agaatataga gaaatgaaac
    24181 aaatggaaaa gattaaacaa gcgagactcg aacgtgaatt ggaaagagag cgaaagaaag
    24241 aggctgagct acgtaagaag aagccacatt tgtttaatgt acctcggaaa cattcacgtg
    24301 atccgtactg gttcgatgtc acttataacc aaatgttcaa gaaatggagt gaagcataat
    24361 gagcgtaata agtaacagaa aagtagatat gaacaaaacg caagacaatg ttaagcaacc
    24421 tgcacattac acatacggcg acattgaaat tatagatttt atcgaacaag ttacggcaca
    24481 gtacccacca caattagcat tcgcaatagg taatgcaatc aaatacctgt ctagagcacc
    24541 gttaaagaac ggtcatgagg atttagcaaa ggcgaagttt tacgtccaaa gagcctttga
    24601 cttgtgggat tgatgaccat gatagataac gcacgcaaag aatacttaaa ccaatttttc
    24661 ggatctaaga gatatctgta tcaggataac gagcgagtgg cacatatcca tgtagtaaac
    24721 ggcacttatt actttcatgg gcatatcgtg ccaggttggc aaggtgtgaa aaagatattt
    24781 gatacagcgg aagagcttga aacatatata aagcaacatg gtttggaata cgaggagcag
    24841 aagcaactaa ctttatttta gaggagatgg aaatgatgaa tgctgaaaag catatgcaaa
    24901 tgatgcaaat gttacaaaat tgtgtgattg ataagtatgt atcacacgac gaatacgaag
    24961 agttaattgc catagataag catggtaata aaatgtttat taaattttat ccgaatacgg
    25021 aggatgacac aaatggataa ccgtgaacaa atagaacaat ccgttataag tgctag
//
LOCUS       DALAAR010000039        23585 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000039 DALAAR010000000
VERSION     DALAAR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.041"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            143..1132
                     /gene="ccpA"
                     /locus_tag="O2458_001611"
     CDS             143..1132
                     /gene="ccpA"
                     /locus_tag="O2458_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCZ3887476.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1230..2399)
                     /locus_tag="O2458_001612"
     CDS             complement(1230..2399)
                     /locus_tag="O2458_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCZ3887477.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2424..3056)
                     /locus_tag="O2458_001613"
     CDS             complement(2424..3056)
                     /locus_tag="O2458_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3887478.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3225..4931
                     /gene="acsA"
                     /locus_tag="O2458_001614"
     CDS             3225..4931
                     /gene="acsA"
                     /locus_tag="O2458_001614"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCZ3887479.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5371..7038
                     /locus_tag="O2458_001615"
     CDS             5371..7038
                     /locus_tag="O2458_001615"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCZ3887480.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7334..10021
                     /gene="harA"
                     /locus_tag="O2458_001616"
     CDS             7334..10021
                     /gene="harA"
                     /locus_tag="O2458_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCZ3887481.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10152..10244
                     /locus_tag="O2458_001617"
     CDS             10152..10244
                     /locus_tag="O2458_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887482.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10224..11129)
                     /locus_tag="O2458_001618"
     CDS             complement(10224..11129)
                     /locus_tag="O2458_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ3887483.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11511..12773
                     /locus_tag="O2458_001619"
     CDS             11511..12773
                     /locus_tag="O2458_001619"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCZ3887484.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(12867..14141)
                     /locus_tag="O2458_001620"
     CDS             complement(12867..14141)
                     /locus_tag="O2458_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ3887485.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14313..14939
                     /locus_tag="O2458_001621"
     CDS             14313..14939
                     /locus_tag="O2458_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCZ3887486.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKVYKIT"
     gene            15057..16523
                     /locus_tag="O2458_001622"
     CDS             15057..16523
                     /locus_tag="O2458_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCZ3887487.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16630..17760
                     /locus_tag="O2458_001623"
     CDS             16630..17760
                     /locus_tag="O2458_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3887488.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17721..17816)
                     /locus_tag="O2458_001624"
     CDS             complement(17721..17816)
                     /locus_tag="O2458_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887489.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(17910..19514)
                     /locus_tag="O2458_001625"
     CDS             complement(17910..19514)
                     /locus_tag="O2458_001625"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ3887490.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19501..20661)
                     /locus_tag="O2458_001626"
     CDS             complement(19501..20661)
                     /locus_tag="O2458_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCZ3887491.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20775..21221)
                     /locus_tag="O2458_001627"
     CDS             complement(20775..21221)
                     /locus_tag="O2458_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCZ3887492.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21301..22044
                     /locus_tag="O2458_001628"
     CDS             21301..22044
                     /locus_tag="O2458_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ3887493.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22260..22862)
                     /gene="rpsD"
                     /locus_tag="O2458_001629"
     CDS             complement(22260..22862)
                     /gene="rpsD"
                     /locus_tag="O2458_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCZ3887494.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23106..23570)
                     /locus_tag="O2458_001630"
     CDS             complement(23106..23570)
                     /locus_tag="O2458_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCZ3887495.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
BASE COUNT         8066 a         3545 c         4287 g         7687 t
ORIGIN      
        1 tttagcgttt gtttttattt caagcttttc gctaaatttt tccaaacaaa aatatgttac
       61 tgtaaattaa aatatggtaa actatgaaaa tgaaatgaaa acatgttatt ataatgaata
      121 aaacgtttac aaggaggaaa ttatgacagt tactatatat gatgtagcaa gagaagcgcg
      181 tgtctctatg gccacagtgt cgcgtgttgt taatgggaac caaaatgtta aagcagaaac
      241 taaaaataaa gttaacgaag tcattaagcg tttgaattat cgtccaaatg ctgttgctag
      301 aggtttagct agtaaaaaga caacaacagt aggtgtgatc attccagata tatctaatat
      361 ctattattca caacttgctc gtggacttga agatattgca acaatgtata aatatcactc
      421 aattatttca aattcagata acgatcctga aaaggaaaaa gaaattttta ataacttatt
      481 aagtaaacag gttgatggta ttattttcct tggtggtaca attactgaag aaatgaaaga
      541 attgataaat caatcatctg tacctgtagt agtatcagga acaaatggta aggatgcaca
      601 tatagcatca gttaatattg attttactga agctgcgaaa gaaattacgg gagaattaat
      661 tgaaaaaggc gctaaatcat ttgctttagt aggtggagaa cattctaaaa aagctcaaga
      721 agatgtttta gaaggtttaa ctgaagtgtt aaataaaaat ggccttcaat taggtgatac
      781 attgaattgt tctggtgctg aaagttataa agaaggcgta aaagcttttg ccaaaatgaa
      841 aggcaatttg ccagatgcca ttttatgtat cagcgacgaa gaagcaattg gtattatgca
      901 tagtgcaatg gatgctggta ttaaagttcc agaggaatta caaattatta gtttcaataa
      961 tacacgatta gttgagatgg ttagaccaca actttctagt gttattcaac cattatatga
     1021 tatcggtgca gtagggatgc gcttattaac aaaatatatg aacgatgaaa agatagaaga
     1081 accaaatgta gttttacctc acagaattga ataccgagga actacaaaat aaattcacaa
     1141 aattaggcat tcatctaacg acccaaatta tatgggtgtt ggaagaatgc cttttattta
     1201 tcttttaaaa tcgttgcaga ttaggttact tattgacgag tagattcgta ccaactcgct
     1261 atatgtaaag ctaatttttt atttttttca ctaatttctt ttgtgcgggg gacataggta
     1321 taatcattta aacgatcttc ccatctttta ggtaataatt cagatgaata atgtttccat
     1381 ttattaatcc attctaacgg taaataacca ctttgaattg gttgatcaat taaacttaag
     1441 aatacatgac tccatgcacg tggtacgact ctccaaatat tgtagcctcc gccaccaaac
     1501 ataattacct ttccattcgt ataagaatca gctaaatatt ttacaaaata tggaatttca
     1561 tataatgaat gtaacgtaca atttagatga gttagtggat cacgataatg tatatcgaca
     1621 ccatttacgc ttagaataat atcaggttta aaactcttta cgacaggctc aactgttaat
     1681 ttaaaacact ccaaaaatga tgcatcttct gtatacggtt caagtgggac atttacagtg
     1741 tgtccatagc cgatatcttc accgcgctca gtatagtgac cagagcctgg gaaaagaaat
     1801 tttccggttt catggataga ataagtagta acatggttat cggcatagaa actccattgt
     1861 gtaccatctc catgatgtgc atcggtatct atgattaaaa cgcgttgatt gtattcttta
     1921 gctaagtatt gtgcggtaat tgcaatatca ttgtatatac aaaaaccact tgctcgacca
     1981 ggttgagcgt gatgcaaacc accacctaag tgacaaccat ttaatacttt gcctgacata
     2041 ataagatctg ctaaagttaa agcgcctcca acaattgtgg cactatggcg gtgcatatgc
     2101 ttaaattgac cattctcttc atcatttaat ccatatttct tagcctcatc ttcactgata
     2161 atgccatgtg aagcatgctt aatagcttcg acgtaatcat atttatgaat taacattaat
     2221 tcgtcatctg ttgcaattct aggttgtact atttgttctg gagacaataa atttgcattc
     2281 aaaagtagct ctgttgttaa ttttaaacgc atttgattga agggatgttg gtcatgaaat
     2341 cgatattgta ataacttatc tgaataaaca tatgcagttt ttgatgaatg ttgttgcata
     2401 taatccctcc gatattccaa aaattaaaag aaaaaccgat tcatataacg aatatcatca
     2461 aacgcttgtt gctgttctaa tgtaatgttt ttgccaattc ttgccattaa acaattagct
     2521 ggatgacttg ttatttctgg atcatctgta gcgaatattt caagtccacc agttgccatt
     2581 aaccgctgca ttaatttttt atagtcaaat acatctaact ttgaattttt taaatcccaa
     2641 tgccagtaat attctgtagt tataacgata taattctcga attctggtgt agaaaggcta
     2701 agttgtatca gcttttctgc aagttgtaat tgcctaaaat tgatgctgac ttcaattgcc
     2761 cccaattcga ttaaatatgg aagcttacct gtagaccagc gctcaatttc atcaggatag
     2821 tggaaagtga cataacccac aatgagttga tcttggcgag caacataaat tctaccttct
     2881 ggtaatgttg taatttctaa caaagcttta tactgatctt cagcatctct aaatgcggtt
     2941 aaatgcgcat cgaaagtaag cgctttcaaa tcttcgtgtg ttaaaggacc ttcaataaca
     3001 aattgcttgt catgaatgta ataatcttcg gattgatacg tctttaaatg attcatattt
     3061 tcaactcctc aatcgacgtc gtgattgtat taaattcatt atatagaaaa tttacaataa
     3121 ttaatactag aaaaaggata aaagtaaaaa ttttgaataa ttagaaatgt tatgtataat
     3181 attgagaaag aaagcgtttt cacataacaa agggggagtt tcaaatgaaa gtcgaagttt
     3241 ataaaggagc gcaaggtaaa cataacctta aagattatga agaaacatat aatacttttg
     3301 attggaaaga cgtagaacaa gcattttctt ggagtgaaac tggaaaaatg aacatggcat
     3361 atgaatgcat agatcgccat gtagatcaag gattagggga taaaatagcg ttaaattaca
     3421 aagatgagca cagaaaagaa tcgtatactt ataaagatat gcaacggtta tctaataaag
     3481 cagcgaatgt tttgtctgaa catgcagaag ttgacaaagg tgacagagta tttatattta
     3541 tgtcgcgtac acctgaacta tattttgcgt tgttaggtgt tttaaaaatt ggtgcaattg
     3601 ttgggccgtt atttgaagca tttatggaaa aggcagttgc ggatagatta gagaacagtg
     3661 aagctaaagt gttaattact aataaggcat tgttacctcg agtacctgta gataaattac
     3721 caaacttgaa aaaaattgtt gtcgtagatg aggatgtaga agacaattac atagacttca
     3781 ttagtttgat ggaaactgct agcgatgaat ttgacattga atggttaaag tcggatgatg
     3841 gtttgatttt acattataca tcaggttcta ctgggcaacc taaaggtgta ttgcatgttc
     3901 aacaagcaat gttagtgcac tatatttctg gaaaatatgt attagattta caagaagatg
     3961 atgtttattg gtgtacagca gatccaggtt gggttacagg aacatcttat ggtatttttg
     4021 caccatggtt aaatggcgct acaaattgta tagctggtgg tcgcttttcg ccagaacagt
     4081 ggtatagtat gattgaagat tttaaagtga cgatttggta tacggcacca acagctttaa
     4141 gaatgttaat gagtgctggt gacgatattg ttgagaaata tgacttgtca tcgttacgtt
     4201 cgattctatc agtaggtgag cctttaaatc ctgaagttat aaaatgggcg aaaaaagtat
     4261 acggtttaac ggtgttagat acttggtgga tgacagaaac aggtggacat atgattgtta
     4321 actatccaac gatggacgtc aagcttggct caatgggcaa accattacct ggtattcaag
     4381 ctgcaattat cgatgatgca gggaatgaat taccaccaaa tcgaatgggc aaccttgcta
     4441 taaaaaaagg ctggccatca atgatgtatc gtatctggaa gaatccagaa aaatataaat
     4501 catattttat tggagactgg tatgtatctg gtgattcggc atataaagat gaagatggtt
     4561 acttctggtt ccaaggacgt gttgatgatg taattatgac agctggtgaa cgagttggac
     4621 catttgaggt tgagtctaaa ttggttgaac acgaagcagt tgccgaagca ggaattattg
     4681 gtaaacctga tccggttcgc ggtgaaataa ttaaggcgtt tgttgcactg agaaaaggat
     4741 atgaaccaac agacgaatta aaagaagaaa ttcgtatatt tgttaaagaa ggtttgtcgg
     4801 cacatgcagc accacgtgaa atcgaattta aagataaatt acctaaaaca cggtcaggta
     4861 aaattatgag acgtgtatta aaagcttggg aattaaattt agatgctggg gatttaagta
     4921 caatggaata atgacatgaa tgttattgaa gatttttttc gaagaataaa gggtgacaac
     4981 atatttcatg tcaatgttta aataatcgtt tactttacga taagcaatat aaagaactgt
     5041 taacttgtgt catatcattt cgtagaaagc atttgaaaat gatgacataa caataatggc
     5101 atatctttat attgcttttt atttttaata tgatctttgg aagatgatta ttttaaataa
     5161 tagaaaaata tagttatcaa tagtatcaag cgctaaaagt tgtataatac aaaactttaa
     5221 taagtgaatt tattgcaaaa atgaaagcgc taacccgatt tagtcgacaa gtttttaaca
     5281 gttcgttatt atatgaatgt aagtaaaaat ttcttagcta caacttacat attataaatg
     5341 cataaattaa acaaaaaggg gcgaaaaaag ttgactcatt tatcagattt agatattgcg
     5401 aatcaatcaa cactacaacc aattaaggat attgctgcat cagtaggtat ttcagaggat
     5461 gcattagaac cttatggtca ttacaaagct aaaatcgaca ttaataaaat tacgccaaga
     5521 gaaaacaaag ggaaagttgt tttagtaact gcgatgagcc caacaccagc tggtgaaggt
     5581 aaatcaacgg ttacagttgg tttagctgat gcattccatg agttaaataa aaacgttatg
     5641 gttgcattaa gagagcctgc tttaggacca acatttggta tcaaaggtgg tgcgactggt
     5701 ggtggttatg cgcaagtctt acctatggaa gatatcaact tacatttcaa cggagatttc
     5761 catgcgatta caactgcaaa taatgcattg tctgcgttta tcgataatca tattcaccaa
     5821 ggtaacgaat taggaatcga tcaaagacgt attgagtgga aacgtgtatt agatatgaat
     5881 gatcgtgcac ttagacatgt aaacgttggg ttaggtggac ctacaaatgg tgtaccacgt
     5941 gaagatggct ttaatattac agtagcgtct gaaattatgg cgattttatg tttaagtaga
     6001 agtattaaag acttaaaaga taaaattagt cgtattacta ttggttacac tagagatcgc
     6061 aagccagtta cagttgcaga tttaaaagtg gaaggtgcac ttgcaatgat tttaaaagat
     6121 gcaataaaac caaacttagt acaatcaatt gaagggacac ctgcattagt tcatggtgga
     6181 ccatttgcga atatcgcaca cggttgtaac tcaattttag caactgaaac agcacgtgat
     6241 ttagctgata tcgttgtaac ggaagctgga tttggttcag acttaggcgc tgaaaaattc
     6301 atggacatta aagtgcgtga agcaggattt gatccggcag ctgtcgttgt tgttgcgaca
     6361 attcgtgcgt taaaaatgca tggtggtgta gcgaaagata atttaaaaga agaaaatgta
     6421 gaagcagtaa aagcaggaat tgttaattta gagcgtcatg ttaataatat taaaaaattc
     6481 ggtgtagaac cggttgttgc aattaatgca tttatacatg ataccgatgc agaagtagaa
     6541 tatgtaaaat cttgggctaa agaaaataac gtacgaattg ccttaactga agtttggaaa
     6601 aaaggtggta aaggtggcgt tgacttagca aatgaagtat tagaagtcat tgatcaacct
     6661 aattcattta aacctttata tgaattagaa ttaccattag agcaaaagat tgaaaagatt
     6721 gtgactgaaa tctatggcgg ttcaaaagta acgtttagca gtaaagcgca aaaacaatta
     6781 aaacaattta aagaaaatgg ttgggataat tacccagtat gtatggcgaa aacacaatat
     6841 tcattctcag atgatcaaac gttgttaggt gcaccatcag gatttgaaat tacaattcgt
     6901 gaattagaag cgaaaacagg tgcaggattt atcgtagcgt tgacaggtgc aatcatgact
     6961 atgcctggtt tacctaaaaa accagcagca ttaaacatgg atgttactga tgatggtcat
     7021 gcaattgggt tattctaata aatcatgtca attgtttaat aaagataagt aaatagttta
     7081 atagaccgga ctgttggaga tgcattattt cagcagttcg gttttttgct gtgctaaaaa
     7141 tagattcaat ttggcgaatc taacgacaat gtttgaaggt ggttaattaa tgtatatgaa
     7201 gataaaaagt gggcttgaag aataggaaag cgatgcaatg aatattccat attaaaaaaa
     7261 aataataaaa taggttgcaa tatttaattg ggatgcgcta caattaacac taataatagg
     7321 agttgttaac aacatgaaca aacatcaccc aaaattaagg tctttctatt ctattagaaa
     7381 atcaactcta ggcgttgcat cggtcattgt cagtacacta tttttaatta cttctcaaca
     7441 tcaagcacaa gcagcagaaa atacaaatac ttcagataaa atctcggaaa atcaaaataa
     7501 taatgcaact acaactcagc cacctaagga tacaaatcaa acacaacctg ctacgcaacc
     7561 agcaaacact gcgaaaaact atcctgcagc ggatgaatca cttaaagatg caattaaaga
     7621 tcctgcatta gaaaataaag aacatgatat aggtccaaga gaacaagtca atttccagtt
     7681 attagataaa aacaatgaaa cgcagtacta tcactttttc agcatcaaag atccagcaga
     7741 tgtgtattac actaaaaaga aagcagaagt tgaattagac atcaatactg cttcaacatg
     7801 gaagaagttt gaagtctatg aaaacaatca aaaattgcca gtgagacttg tatcatatag
     7861 tcctgtacca gaagaccatg cctatattcg tttcccagtt tcagatggca cacaagaatt
     7921 gaaaattgtt tcttcgactc aaattgatga tggagaagaa acaaattatg attatactaa
     7981 attagtattt gctaaaccta tttataacga tccttcactt gtaaaatcag atacaaatga
     8041 tgcagtagta acgaatgatc aatcaagttc agtcgcaagt aatcaaacaa acacgaatac
     8101 atctaatcaa aatacatcaa cgatcaacaa tgctaataat caaccgcagg caacgaccaa
     8161 tatgagtcaa cctgcacaac caaaatcgtc aacgaatgca gatcaagcgt caagccaacc
     8221 agctcatgaa acaaattcta atggtaatac taacgataaa acgaatgagt caagtaatca
     8281 gtcggatgtt aatcaacagt atccaccagc agatgaatca ctacaagatg caattaaaaa
     8341 cccggctatc atcgataaag aacatacagc tgataattgg cgaccaattg attttcaaat
     8401 gaaaaatgat aaaggtgaaa gacagttcta tcattatgct agtactgttg aaccagcaac
     8461 tgtcattttt acaaaaacag gaccaataat tgaattaggt ttaaagacag cttcaacatg
     8521 gaagaaattt gaagtttatg aaggtgacaa aaagttacca gtcgaattag tatcatatga
     8581 ttctgataaa gattatgcct atattcgttt cccagtatct aatggtacga gagaagttaa
     8641 aattgtgtca tctattgaat atggtgagaa catccatgaa gactatgatt atacgctaat
     8701 ggtctttgca cagcctatta ctaataaccc agacgactat gtggatgaag aaacatacaa
     8761 tttacaaaaa ttattagctc cgtatcacaa agctaaaacg ttagaaagac aagtttatga
     8821 attagaaaaa ttacaagaga aattgccaga aaaatataag gcggaatata aaaagaaatt
     8881 agatcaaact agagtagagt tagctgatca agttaaatca gcagtgacgg aatttgaaaa
     8941 tgttacacct acaaatgatc aattaacaga tttacaagaa gcgcattttg ttgtttttga
     9001 aagtgaagaa aatagtgagt cagttatgga cggctttgtt gaacatccat tctatacagc
     9061 aactttaaat ggtcaaaaat atgtagtgat gaaaacaaag gatgacagtt actggaaaga
     9121 tttaattgta gaaggtaaac gtgtcactac tgtttctaaa gatcctaaaa ataattctag
     9181 aacgctgatt ttcccatata tacctgacaa agcagtttac aatgcgattg ttaaagtcgt
     9241 tgtggcaaac attggttatg aaggtcaata tcatgtcaga attataaatc aggatatcaa
     9301 tacaaaagat gatgatacat cacaaaataa cacgagtgaa ccgctaaatg tacaaacagg
     9361 acaagaaggt aaggttgctg atacagatgt agctgaaaat agcagcactg caacaaatcc
     9421 taaagatgcg tctgataaag cagatgtgat agaaccagag tctgacgtgg ttaaagatgc
     9481 tgataataat attgataaag atgtgcaaca tgatgttgat catttatccg atatgtcgga
     9541 taataatcac ttcgataaat atgatttaaa agaaatggat actcaaattg ccaaagatac
     9601 tgatagaaat gtggataaag atgccgataa tagcgttggt atgtcatcta atgtcgatac
     9661 tgataaagac tctaataaaa ataaagacaa agtcatacag ctgaatcata ttgccgataa
     9721 aaataatcat actggaaaag cagcaaagct tgacgtagtg aaacaaaatt ataataatac
     9781 agacaaagtt actgacaaaa aaacaactga acatctgccg agtgatattc ataaaactgt
     9841 agataaaaca gtgaaaacaa aagaaaaagc cggcacacca tcgaaagaaa acaaacttag
     9901 tcaatctaaa atgctaccaa aaactggaga aacaacttca agccaatcat ggtggggctt
     9961 atatgcgtta ttaggtatgt tagctttatt cattcctaaa ttcagaaaag aatctaaata
    10021 attaactaaa tatagcatat gtatgattaa ctttgtagac aatgtgaaag caattaattt
    10081 ataaactatt gattggttta atggctttcc tttagagtaa ataaaaagaa cagcagtgag
    10141 aaattttcta attgaaaata atcttactgc tgtttttaat atttggattc attgttgtgg
    10201 ttactttaaa aagtgagcat caattaacgc ttttttcgat ttaacaaatg tgatttaata
    10261 tcatatttta atgcgtcgtt gtattctttt tcagtgattt gatcttcgat taacatacgc
    10321 tttaatacat aatgttgtct ttgaatacta tatttcaaat ctttatccga ttttaacgtt
    10381 ccatcttttt cgtagggtgt atagccatat gggctttgca acaaaccgat aaggtatgca
    10441 gattgtgcaa ttgataaatc ttttggtgga ataccaaata gactatatga agcggatgca
    10501 attccggaaa tattagcgcc attataatct ctaccgaagg gaactatatt taaatatgta
    10561 tatataattt catcttttga gagtaggtgt tctaatctaa ttgctaggcg aagttcattt
    10621 gcttttctac tatatgtttt ttcgttggta agaacttgat ttttaacaag ttgttgtgta
    10681 attgtgctac cacctgaact ttgatcagta ttaaaaatat cttgtatcat tgctcttaaa
    10741 atcgcctttg gtaagatgcc atcatgttta taaaataaag tgtcttcaga tgacgttaat
    10801 gctttaatga catttggact tgatgtttta gggcctataa tgagtgagtt ttgagaatgg
    10861 ttatactcat ataataaatt tttgttatta tgatctaata attcatcgcc aggtatttgt
    10921 cgaacttttt ttattaaagc atcatctgat aatgagtcgg acgttttagt taaatgatga
    10981 aaatataaag acatcgcaat cacagcgata gcaacgatgg caatgaaaat cataaatata
    11041 aaaacaatga catgtttgat agctttatat atcttttcaa aatgatatat acgatgattc
    11101 aattgatgat tgttgtcttg attcgtcatc tttaacctcc taaattacgt atagtatacc
    11161 atatttaagc aatttgactt ttattttaac gttaattata attagggaaa atgcataaag
    11221 aataggataa aaggacaagt aatattattt gaaatcatca gagagctagt ggtcgctgtg
    11281 aactagtgtt tttaataata tgaatacacc tttataacta acatgtccta cgtttattaa
    11341 tgaacgtatc attaatagag tgaacattat cttatgatta tgtttaagtt tggtggtaac
    11401 gtgcaatcgc gcccttacat atttgttgta agggtttttt ttacgttcaa aaaactttaa
    11461 agtattttaa caagtattca ataaataatt ataaatagga ggaaataata atgacgaatg
    11521 tattaattga agatttaaaa tggagaggtc ttatttatca acaaactgat gaacaaggta
    11581 ttgaagattt attaaataaa gaacaagtga cgttatactg cggtgccgat ccaacggcag
    11641 atagtttaca tattggtcac ttactaccat tcttaacatt aagacgtttt caagaacatg
    11701 gacatcgtcc tatcgtttta attggcggtg gtacaggtat gattggtgat ccatcaggta
    11761 aatcagaaga acgtgtgcta caaacagaag aacaagtaga taaaaatatc gaaggtatta
    11821 gtaagcaaat gcacaatatt tttgaatttg gaacagacca tggtgcagtg cttgttaata
    11881 atagagactg gttaggacaa atctcattaa ttagtttttt acgtgactat ggtaaacacg
    11941 tcggcgttaa ttacatgtta ggtaaagatt caatccaaag tcgtttagaa catggtattt
    12001 catatacaga attcacatac acgattttac aagctattga tttcggtcat ttgaatagag
    12061 aattgaattg taagattcaa gtaggtggat cagatcaatg gggtaatatc acaagtggta
    12121 ttgaattaat gcgtcgtatg tatggtcaaa cagacgcata cggtttaact attccgcttg
    12181 taactaaatc agatggtaag aaatttggta agtctgagtc aggtgctgtt tggttagatg
    12241 ctgaaaaaac aagtccttat gaattttatc aattctggat taatcaatca gacgaagatg
    12301 taattaaatt cttaaaatac tttactttct taggaaaaga agaaattgat cgcttagaac
    12361 aatctaaaaa tgaagcaccg catttacgtg aagctcaaaa aacattagct gaagaagtaa
    12421 ctaaatttat tcatggtgaa gatgcattaa atgatgcaat ccgtatttca caagcattat
    12481 ttagtggtga tttaaaatca ttatcagcga aagaattaaa agatggattt aaagatgtgc
    12541 ctcaagtgac attatcaaat gacacaacaa atatcgttga agtccttatt gaaacaggca
    12601 tttctccttc taaacgacaa gcacgtgaag atgttaacaa tggtgcgatt tatattaatg
    12661 gtgagagaca acaagatgtt aattatgctt tagcaccaga agataaaatt gatggcgaat
    12721 ttacgattat tcgtcgcggt aagaaaaaat acttcatggt taactatcaa taaatataat
    12781 tgcatagcta aataaattag agcctactca tattcattcc taagaatgta atgagtaggc
    12841 tcttaatgta cttttctgtc tgtaaattat ctaaagaaat ctctatcgcc ttggccaggt
    12901 gattgacgtt ctgatcggct ttgacgtttt ggttgttctt tttgttgttt taatttcact
    12961 ttaacttctt ttgttttacc atcacggata acggtaacag tgactgattc accaggtttt
    13021 ttattttcat ataaatagct tcttaaatca acatcatctt taattttctt gccatcaatt
    13081 tctgtaataa tatcaccttt tttaagatca atatcactat cagctttggc gacataaata
    13141 ccgtcttctc tatcagtatg aagttgctcg cgctcttctt caggaatatc tttcaaatta
    13201 attaaaccaa taccaatcga agggcggtca attttaccat gttttacaag ttgttcaatt
    13261 gttactttaa cttcattact tggaatagca aacccgatac cttcaacttg tgtcgcagca
    13321 attttcattg agttaatacc aactaaatta ccattaatat ctactaatgc gccacctgag
    13381 ttacctgggt taatagcagc atctgtttga agaacgctaa cttttgtatt gccaccagtt
    13441 gtctcagcgt caatcgtacg ttcgcttgct gaaatgatac cagatgttac agagttagca
    13501 aattgtaatc ctaatgggtt acccattgcg aatacgctat cgccagtttg tacttttgaa
    13561 gagttggcaa attgaatcgc tttaatacct tttgtatttt caattttaag tacagcaata
    13621 tcagttactg catctttacc aactaatttc gctttaactt gttttttatt atgtaattgg
    13681 actctaattt catttgcgcc atcaataaca tgattgtttg taacgatata agctgaattg
    13741 ttgtttattt gatagataac acctgaacct actccagctt cagatggttt agatgatttg
    13801 ccttttaata agtcgtctac acttgatgct ttttgcatgt taataactcc aacaattgta
    13861 ggggagacag attttatcat ttcatgaacg gtaccgaatt tcttgctttg accgtctaat
    13921 tgattgccac ctttattatt tgttgtctga acagttgaac catctttatt taaaattgta
    13981 ctatttaata ctttgcctat accaagtact agaagtgcac caataattcc agcaatcaat
    14041 gcaacgatga ctgttttaaa ccatggaaat ttaggtcttc tgtatctagg tgtttggcta
    14101 tggtttgttg tagaatgatc tgtatgatta aaatctgaca tacttaacct ccattatatg
    14161 atttatatat gctttaatta tgtctttttt ttataattat tgctagtaat acactataca
    14221 actttagatg tattttcaga ctatcttgtt ctagtaaaaa tatagtgata aaatctagtt
    14281 gttataaaca aataagttaa gtaggtggca caatgtattc agtgattagt aagattttga
    14341 atttcatttt agttaaaatg tcaaaatcat tatacgtaat cggtaaagat aatattccta
    14401 aggatagtaa atatgtcgta acttgtacgc atgaaagtta taacgaagtc attatgctag
    14461 gtatggcatt atatcctaat caaattcatt atatggctaa aaaagaatta ttcaaaaata
    14521 aatggattgg taaattttta acgtctttaa atgcatttcc tgtagatcgt gaaaatccag
    14581 gacctagtac tttaaaacgc ccaatcaatt tgttgaaaga taataagaca gttggtatct
    14641 tcccaacagg ttctcgcaca tcgcaagaag gtgcaccttt gaaacgtggt gcttcaacaa
    14701 ttgcaatgtt aagtaagtca ccaatattac ctgttgctta tgttggtcct actaagattc
    14761 acggtctttt aacaggacaa gcatacataa atattggcaa accaatcgat attaacgatt
    14821 tacctaaaga tttaaaacga aacgagcgta tcgattatat tacaaaagag attgaaacac
    14881 gaacagcaaa gttacaacaa gaattgcatg aaattgtaaa agtttataaa ataacttgag
    14941 gtagaaatat agctggttag taagcctagg tgactataac ggagtatcga agtagttatt
    15001 ctagttacat tagtaatctt tatatttcta cattatttta agaaggatga cattcaatgt
    15061 ataaattttt ccaaaattta gggcgttcgt taatgttacc agttgccatt ttaccggctg
    15121 cagcaattat tgccggaatt ggtaatacgt taaatgcact tcatgcgacg ccgaaaatcg
    15181 caatgttctt tactacggta ggtactacga ttttagagca attaggtatt ttatttgcaa
    15241 ttggtgtagc tataggaatg gcaaagaaaa atgatggtgt agtcgcgctt gcagctacgt
    15301 taggatattt tttagttaca gttgtattat caccaatgaa attagcgcct ttattaggaa
    15361 tgaaagcttc ggaaataaat tcagcttttg aaaagatgaa taatggcaat gtctttgttg
    15421 gtattgtaat aggtttaata gcagcttatg catacaataa gtttagtgaa acagaattac
    15481 cattagcatt atcatttttt agtggtaaac gtctagttcc aattatgact gcattttact
    15541 gtacattttt agttgtcata ttgttattct tatggccact actttattca tggattgtaa
    15601 aatttggtga atctatagtc ggtttaggat cgtttggagc tttcatatat ggtgttgcca
    15661 atagattatt aattcctaca ggattacatc atgcactgaa tagtgtgttc tggtttgata
    15721 cgattggtat taatgatatt ggtaaattcc aaagtggcaa agatgctata aaaggtatta
    15781 caggacgcta tcaagcggga ttcttcccaa tcatgatgtt cggtattcca gcggctgcgt
    15841 tagcaatgta tcatacagct aaaactactc agaaaaaaca agtttacggt tggttcctag
    15901 cgagttcagt tgcagcattt ttcgttggtg ttacagagcc gattgagttt gcatttatgt
    15961 ttgttgcgcc aatcttatat gttgttcatg cgttattaac aggattatct ttatttatcg
    16021 cagctacatt ccattggaca gctggatttt catttagtgc aggtttgata gattatgtgt
    16081 tatcactaat taatccagta tcaaatcatc cattgatgtt gcttgttcaa ggtgttgtat
    16141 ttttcatctt atactatgtg attttccgtg tagtaatcca agtatttaac ttgaatacga
    16201 ttggtagagg tgaaaatgaa ttagttgacc caacagttgt aaaagataat attgctcctg
    16261 gtgaaaatga tattaaacaa agtaaatatc atcaacatgc tatacaaata ttagaaggtt
    16321 taggcggtca agagaatatt gttaatttaa ccaattgtgc aacaaggttg cgtctagagt
    16381 taaaagacac atctattatt gataaacaga agattaaaaa cgccggtgca gttggtgtta
    16441 ctgttaatgg caaacattca actcaagtta ttgtcggtac acacgtacag caggttgctg
    16501 atgaaattga aaaacattta taaaatttaa acagctaaag cgcaaatcta acttcaatag
    16561 ggtttgcgct ttttttagtc aaatagtaga atgatttata tctgaatttt cagaaatgga
    16621 gtggggattg tgaagaaaat tttatttgat gttgatggcg tttttttaag tgaagaacgt
    16681 tgtttcgatg tctctgcatt aacggtatat gaattattaa tggataagtg ttatttaggt
    16741 cttcattcac atatagattg ggaaactttg actgataacg atatacaaga cattaggaat
    16801 cgaatttttc aaaaagataa gattttaaac aaattaaaat cgctagggct gaattcaaat
    16861 tgggatatgt tatttatcgt ttttagtatt catttaattg atattttgaa aaagttatca
    16921 catgatgaaa ttgaggcatt catgtatcaa gatgagcctg tcgaattgaa gttgcaaaat
    16981 ataagcacaa acttagcaga ctgttttaat ttaaatgaac aactaccttt acaatttttg
    17041 gataatgtaa aagttggtaa aaataatatt tatgctgctt tggaagagtt tgcaacaaca
    17101 gagttacatg tttcggatgc tactttattt agtttgaagg gtgcattatg gacgttagcg
    17161 caagaagttt atcaagaatg gtatttagga tcgaagttgt atgaagatgt tgaaaagaaa
    17221 atagcacgaa ctacttttaa gacaggttat atttatcaag aaattatttt gagaccagta
    17281 gatgaagtta aggtacttct gaatgattta aaaggtgctg gtttcgaatt aggtattgca
    17341 acaggtcgtc cttatactga gactgttgtg ccatttgaaa atttaggatt gttaccatat
    17401 tttgaagctg attttattgc aacagcaagt gatgttttag aagcagagaa tatgtatccg
    17461 caagcacgac cattaggaaa gccgaatcct tttagttata tcgcagcttt atatggtaat
    17521 aatcgcgata aatatgaatc ttatatcaat aagcaagata acattgtaaa taaagatgac
    17581 gtatttatag taggcgattc gttagctgac ttattaagtg ctcaaaaaat aggtgcaacg
    17641 tttattggaa cattaacagg tttaaaaggt aaggatgctg caggtgagtt agaagcgcat
    17701 catgccgact atgttattaa tcatttaggt gaacttagag gtgtactaga taatttgtaa
    17761 tttgattgtt gtttgacagc ataacttgta gtgaatgatt gaaccaaagg tttcatattg
    17821 agttacaatg aaattaataa tgaaaaaatg ccaagaagca atggaagtaa tccaatgtct
    17881 tcttggcatt ttgaatttac ataaattgtt tatgactgta ccgtcaattc agttgtgaaa
    17941 atttgattgt attcaccaac ttgtttaagt tcatcaatta tattgtttga aacaggttga
    18001 tcaacggata aaatcattag cgcatctccg cccgcttcag ttctacctaa agtcatagat
    18061 gcaatgttga tattgtattt acctaacaat gcgccagttt ttcctaccat acctggagta
    18121 tcattatgat atgacacaat ttgatattga tttggcttta agtctacaga aaaattatta
    18181 attctaacaa ttcttggacc gaaacctgta aagacagaag cgccaacttt aacggaatcg
    18241 ctatcgtttg atagttctac ctctaagtag ttactaaaac ctgtctctgc tttattattt
    18301 tcaatattta atgtcacttg ttgttgattt aacaacatta gagcattgat aatattgact
    18361 tcaggaccaa gatcatcttt taaaatatga gtaataattg tacgtgttaa taaactacta
    18421 tcaatagagg ctaagtcgcc accgtacgta attttaatag agcttggtgc attgtacatt
    18481 aattgaatag ctaattcacc aactgtttgg cttaaattga tgaatgattt tacagtatca
    18541 tctatattgc ttaagtccat tttaggtgca ttcactgcat gcgttacagt accatcaatt
    18601 aaaatttcga tgatttcatt tgaaacagaa attgccactt tttcttgagc ttcgactgtt
    18661 gaagcaccca aatgaggtgt aacaataatt ttatcatgtg caacaagagg cgagtcagtt
    18721 gcaggttcat gttcaaacac atcgatagct gcccgactaa tttgtccttc gtctaatgct
    18781 tttattagcg ccttttcatc aataatacca ccacgtgcca cattgattat ttgcaaacta
    18841 ggttttgctt tggcaaaaaa gacagcatta attaagcctt ttgttttagg tgttagtggt
    18901 gtatgtaatg taacgaaatc agaatgttgg gcaatctcat caactgttgc cttcgtaatg
    18961 cttaaagatt ttgctttttc atccgttaag taagggtcaa aagctagtat tttcattccg
    19021 aaactttgcg cacgtttagc aacacctaaa ccaattctac cagcaccaat gacacctaat
    19081 gttttatgat aaagctcagt acctttaaat gcatttcgat tccattcttt atttgtaagt
    19141 gattggtgtg cttgcggaat atttcgtgcc attgataata acattgccag tgtatgttca
    19201 gtagctgaaa tcgtattacc atctggggca ttaataacta aaataccttt taatgttgca
    19261 gcatcaatat taatattatc tacaccaaca ccggcgcgtg cgattacttt taaagaatca
    19321 gcagcattta tgatattttc agtaaccgta gtttgactac gaacgattaa agcatggtat
    19381 gaaggtataa ttttgattaa tgcttcttcg gacaagccag tttgaatatc tacattgaat
    19441 tgttcgtgat ctaataatgc tttgatacca tcttttgata tgggatctgc aacgagtaca
    19501 ttaaattgct tcatgaataa cctccatata ttttgatata cctttaccga tatagttaac
    19561 tttacggtgt tcagttaaaa taatttctaa agcagatact accgataaaa tatcgaaagg
    19621 actaattttc cccatatgac caattcttaa aatttgacct ttaagatggc cttgaccacc
    19681 agcaattgtt attttaaagc gatttttaag ttcatctttg attattttca cttcatcatt
    19741 tgtattaggt ttgaatgctg taaccgttgg agatgcatct ttatcattga ctaataaagt
    19801 taaatctaat gcttttaagg cgcttcttaa tgcatttcta attgcatagt gtcgtgctat
    19861 tacgtgattg aaaccttctg cttttacggt ttcaacgtat gcatttacac ctctaaataa
    19921 agacacattt ggtgtgaacg gtgtagaatt gtcagcttgt gacgaaatgt atttatttaa
    19981 gtctagataa aattttggcg tagttacttc tttgaaatgt tcttttgcac ggtggctata
    20041 agctacaaaa gctaatcctg gaggtaacat aattgctttt tgactaccag aaacaagtac
    20101 atcaatttta tctttgttaa tgtcaacatc aacagcacca atgcaactta cgccgtcaac
    20161 tacaaaataa atattactat taaattgatt aatggcattt cctaactcgt gaatagggtg
    20221 tagcactgtc gtagatgttt cgcaatattg actaaatact gctttaactt caacatttaa
    20281 agttgaaagg aaattgatga aatctttgac atctacagct tctccccatg ttacgtcata
    20341 aatatgcaca tttttgtaat aagtttgtgc aatttgttta aatcggttac caaaggcacc
    20401 tgaaacaatg acaacgaagt gatcttcagg gtttacaatg ttcaacatac tagcctccaa
    20461 gacgcttgta ccgctagatg ttaaaataag tacatcattt tgactcccaa atattggctt
    20521 tagaccttga aatgcttgtt gtgcgatgtc ttcaaaatct ttagaacgat gaccaaccat
    20581 aggtgcttga atttctctca taatggcatc aggtacaggg gttgggccag gtgttaataa
    20641 caacggttga tgataataca tttatatgcc tcctataaat aagatttcac tattttagca
    20701 aattttctga aaatttaaaa gcctaaaaaa gtgataacgc tatattgtcg aacaaacgtt
    20761 atcacttaac gattttattt ggcctgaatg atgggataga tttttatgtc cacattattt
    20821 cttacagcat ttgaaatcat gcaattatta tctgcaattg ttatcaattt tggtaatcgc
    20881 ttttctaatt gtgctatttg atcacttgga atttgaattt gaggatggtg cacaattttt
    20941 gacatactga attttccgtt atttaaacaa gctgttccaa tcgattgttg ttcaattgaa
    21001 atatctgtga actttgcacg ttcaagagta gctgctaatg agatgatata acatgatgaa
    21061 gcggctgata ctaacatttc atcgggattt gttcctatac caacaccacc taaagaagca
    21121 ggtatagaaa tattctctga aagtatgtcg ccttgaacgt ttccgacatt gttacgacca
    21181 ccttgccaag aagtttggac tttaaagtca tgttgatgca attgctttaa cctccaatat
    21241 aattgtgata gtttaatttt agaatagttt atcaaagttt aacagaaagg tgacttatca
    21301 atgactctga ataaactgaa agatgaatta caaattgttt cgcaccgtgg attgccgagt
    21361 gattttcctg aaaatacaat ggtcggttat cgagaggtaa tggggctcaa tgttgctatg
    21421 ttagaaatag atgttcattt gaccaaagac caacattttg ttgtgataca tgatgaaaca
    21481 attgatagaa catcggatgg taaggggcgt attgctgatt acacattatc gcaattaaaa
    21541 tcatttgatt ttggtagtta taaagatgtt gcttttaagg gagaacgaat acccacgtta
    21601 gatgaagtac tttcattatg cttaaagtat gataaaaagc tattgattga attgaaaagt
    21661 ccgaatttat atccagaaat agaatgtaaa ttgctggcat ttttggaaga aaagaaagtc
    21721 gatgcgacac aagtagttat acaatctttt gatatcgaat gcatcgaaaa gttaaacaca
    21781 ttgggcagta tatatgaatt aggtgtgctt tgtagtaaac gtaattattg gtataaaaag
    21841 ccaaactttt caagaattgc tcaaattgct agttatgtta atcctaatta tgcattagtg
    21901 accagaaaat ttgttgataa agcacatcat catcaattac aggttatgcc atacacagtt
    21961 aataaattga aaactggaga aaaattaaga caaatgggtg tagatggatt aattacagat
    22021 aatccgaaat tatttattaa gtgagacgat gaatggtaat ttgatagagg gatgaggatt
    22081 cgtatatcgc aattgataat tttaaaaact aatgatgaaa tactaaagat aattaaacaa
    22141 ataagacttg taaattctaa gttgattgta aaataggata gctccatata attttgaatc
    22201 tatcattttt aaattaataa aaaaaacacc cacaattgtg ggtgtttgta ttgaacgtat
    22261 tatcttgagt agtactcaac gattaattgt tcgttaattt cagcaggtaa ttcgctacgt
    22321 tctggtaaac gtacgaaagt accagttaag ctgtcagcat caaagtttaa gtactcaggt
    22381 acgaaattgt tgatttcaac tgattcaacg atgatgttta atttttgaga tttttcacga
    22441 actgaaattg tttgaccagg tttaacagaa taagatggaa tatcaacacg tttaccatct
    22501 actaagatat gaccgtggtt aactaattga cgtgcttgac gacgagtacg agctaaacct
    22561 aatgaataaa caacagcgtc taaacgactt gctaataaga tcatgaagtt ttcaccgtgt
    22621 acaccgaatt ttttaccagc gatgtcaaat gtgttacgga attgtctttc agtcattcca
    22681 tataagtaac gtaatttttg tttttcacgt aattgtaaac catattctga taatttttta
    22741 cgttggtttg gaccatgttg tcctggtgcg taaggacgtt tttctaattc tttaccagta
    22801 ccgcttaaag agatacctaa acgacgagat tttttccagt ttgaacctct gaatcgagcc
    22861 ataataagac tcctccttta attctttttt gttgttatga ataaacaaaa aagagtgttg
    22921 catgcttact aagatatatt gttttatgtg tcttcacctt atagcgacag ttacgtgaca
    22981 cgtccgcatt gggaacaaca tagcgctcaa taatatacaa ctgctacttt cgttaattca
    23041 cacaaagatt attgtaacat tttcctgcta actgtcaaca ctttattgaa aatgctgatg
    23101 tccttttatg cgagttgctt ttcaataatt ttaacaattg cttcaagatg ttctttgtca
    23161 ttgtcatcaa atcgatccgt tataggggca tcgatatcta agacgccgat aattttatca
    23221 tctttgaaaa ttggaacgac aatctcagac ttactattag catcacaagc gatatgtcct
    23281 tcgaattgat gaacatcagc tacaatttgt gtacgacgtt ctgaaacggc tgtaccacat
    23341 acaccttttc caattggaat gtggacacaa gcggggtgtc cttggaaagg tccaagtata
    23401 agttcatttt gttccaataa gtaaaagcca acccaattaa tttgatctag attatcattt
    23461 aataaggcag acatgttact taaaatagca atcatgtgat gttcatcttc aataaggctt
    23521 gctgcttgtt tctttaataa tgtgtagttt gttgggttaa ttgttgtcat atttaaaacc
    23581 tctta
//
LOCUS       DALAAR010000040        23325 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000040 DALAAR010000000
VERSION     DALAAR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.042"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..423
                     /locus_tag="O2458_001631"
     CDS             <1..423
                     /locus_tag="O2458_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCZ3887496.1"
                     /translation="EPQEKAVIKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKID
                     AGYHGNLGINIKNDNETLESEDMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKG
                     DKLAQLVIVPIWTPELKQVEEFESVSERGAKGFGSSGV"
     gene            460..705
                     /locus_tag="O2458_001632"
     CDS             460..705
                     /locus_tag="O2458_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887497.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            702..908
                     /locus_tag="O2458_001633"
     CDS             702..908
                     /locus_tag="O2458_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ3887498.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            905..1291
                     /locus_tag="O2458_001634"
     CDS             905..1291
                     /locus_tag="O2458_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887499.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            1288..1437
                     /locus_tag="O2458_001635"
     CDS             1288..1437
                     /locus_tag="O2458_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3887500.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            1437..1637
                     /locus_tag="O2458_001636"
     CDS             1437..1637
                     /locus_tag="O2458_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ3887501.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            1665..2081
                     /locus_tag="O2458_001637"
     CDS             1665..2081
                     /locus_tag="O2458_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3887502.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            2313..2612
                     /locus_tag="O2458_001638"
     CDS             2313..2612
                     /locus_tag="O2458_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ3887503.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            2742..3086
                     /locus_tag="O2458_001639"
     CDS             2742..3086
                     /locus_tag="O2458_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887504.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            3083..4744
                     /locus_tag="O2458_001640"
     CDS             3083..4744
                     /locus_tag="O2458_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ3887505.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            4760..5947
                     /locus_tag="O2458_001641"
     CDS             4760..5947
                     /locus_tag="O2458_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ3887506.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            5931..6668
                     /locus_tag="O2458_001642"
     CDS             5931..6668
                     /locus_tag="O2458_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ3887507.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            6692..7837
                     /locus_tag="O2458_001643"
     CDS             6692..7837
                     /locus_tag="O2458_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ3887508.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            7857..8141
                     /locus_tag="O2458_001644"
     CDS             7857..8141
                     /locus_tag="O2458_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887509.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            8131..8415
                     /locus_tag="O2458_001645"
     CDS             8131..8415
                     /locus_tag="O2458_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCZ3887510.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            8399..8761
                     /locus_tag="O2458_001646"
     CDS             8399..8761
                     /locus_tag="O2458_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCZ3887511.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            8758..9162
                     /locus_tag="O2458_001647"
     CDS             8758..9162
                     /locus_tag="O2458_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCZ3887512.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            9159..9563
                     /locus_tag="O2458_001648"
     CDS             9159..9563
                     /locus_tag="O2458_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887513.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            9567..10211
                     /locus_tag="O2458_001649"
     CDS             9567..10211
                     /locus_tag="O2458_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ3887514.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            10238..10477
                     /locus_tag="O2458_001650"
     CDS             10238..10477
                     /locus_tag="O2458_001650"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCZ3887515.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            10527..10877
                     /locus_tag="O2458_001651"
     CDS             10527..10877
                     /locus_tag="O2458_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887516.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            10928..11065
                     /locus_tag="O2458_001652"
     CDS             10928..11065
                     /locus_tag="O2458_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887517.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            11122..15651
                     /locus_tag="O2458_001653"
     CDS             11122..15651
                     /locus_tag="O2458_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCZ3887518.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            15648..17132
                     /locus_tag="O2458_001654"
     CDS             15648..17132
                     /locus_tag="O2458_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ3887519.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            17148..20933
                     /locus_tag="O2458_001655"
     CDS             17148..20933
                     /locus_tag="O2458_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887520.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            20923..21075
                     /locus_tag="O2458_001656"
     CDS             20923..21075
                     /locus_tag="O2458_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887521.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            21122..21409
                     /locus_tag="O2458_001657"
     CDS             21122..21409
                     /locus_tag="O2458_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887522.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            21467..21763
                     /locus_tag="O2458_001658"
     CDS             21467..21763
                     /locus_tag="O2458_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ3887523.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            21913..22089
                     /locus_tag="O2458_001659"
     CDS             21913..22089
                     /locus_tag="O2458_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCZ3887524.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(22142..22249)
                     /locus_tag="O2458_001660"
     CDS             complement(22142..22249)
                     /locus_tag="O2458_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887525.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            22301..22555
                     /locus_tag="O2458_001661"
     CDS             22301..22555
                     /locus_tag="O2458_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ3887526.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            22567..23322
                     /locus_tag="O2458_001662"
     CDS             22567..23322
                     /locus_tag="O2458_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887527.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT         8700 a         3166 c         4843 g         6616 t
ORIGIN      
        1 gagccacaag aaaaggcagt gatcaaaaca gatgtagctg taagcatacc agagggctat
       61 gtcggactat taactagccg tagtggtgta agtagtaaaa cgcatttagt gattgaaaca
      121 ggcaagatag acgcgggata tcacggcaat ttagggatta atatcaagaa tgataatgaa
      181 acgttagaga gtgaggatat gagtaacttt ggtcggagtc ctgctgggat agatggaaag
      241 tatgcccgac tacctgtaac agataaaatt ttatgtatga atggtagtta tgtcataaac
      301 aaaggcgaca aactagctca attggttatt gtgcctatat ggacacctga actaaagcaa
      361 gtggaggaat tcgagagtgt ttcagaacgt ggagcaaaag gcttcggaag tagcggagtg
      421 taaagacata ttagatcgag tcaaggaggt tttggggaag tgagcgacat gttagaaata
      481 tttttaatag ggtttggcgt ttatctcttt tatcgcatag caattatttt tcttaagagt
      541 aaaaagacta tacacacaaa catatatgaa atgttaatgc ttgctactat ctttatgata
      601 tctacatttg cttataaaca tcaaaagacg catatcttaa tagcattttt agtaatgttt
      661 tttatgagta agctcaaaca agttcaaggg agctatgagg aatgacacaa tacttagtca
      721 caacattcaa agattcaaca ggacgtaaac atacacacat aactaaagct aagagtaatc
      781 aaaggtttac agttgttgag gcagagagta aagaagaagc gaaagagaag tacgagaaac
      841 aagttaaaag ggatgcagtt attaaagtgg gtcagttgtt tgaaaatata agggagtgtg
      901 ggaaatgatt aaaaaactta aaaatatgga tgggttcgac atctttattg ttggaatact
      961 gtcattattc ggtataaccg cattgctact tgttgtcgca ttgcctatct atacagtggc
     1021 tagttaccaa aacaaagaag tacatcaagg gacaattaca gataaatata acaaaagaca
     1081 agataaagag gacaaattct atattgtatt agatgataaa caagtcatcg aaaactcaga
     1141 cttattcttc aaaggaaagt ttgatagcgc agacatacaa gctaggttaa aagtaggtga
     1201 taaagtaaaa gttaagacga ttggatatag aatacacttt ttaaatttat atccggtctt
     1261 atacgaagta aagaaggtag ataaaaaatg attaagcaaa tactaagatt attattctta
     1321 ctagcgatgt atgagttagg taagtatgta actgagcaag tatatattat gatgacggct
     1381 aatgatgatg tagaggcgcc gagtgattac gtctttcgag cggaggtaag tgagtgatgt
     1441 ggattactat gactattgta tttgctatat tgctattagt ttgtatcagt attaatagtg
     1501 atcgtgcaag ggagatacaa gcgctcagat atatgaatga ttatctactt gatgaagtag
     1561 ttaaaactaa aggatacaac gggttaaaag aatacaggat tgaattaaag cgaatgaata
     1621 acgatattaa aaagtaattt atattatcgg aggtattaca ttgaatgata aagattgaga
     1681 aacacgatat caaaaagctt gaagaataca ttcagcacat cgataactat cgaagagagt
     1741 tgaagatgcg agaatatgaa ttacttgaaa gtcatgaacc agataatgcg ggagctggca
     1801 aaagtaattt gccgggtaac ccgattgaac gatgtgcaat aaagaagttt agtgataaca
     1861 ggtacaatac attaagaaat atagttaacg gtgtagatag attgatagat gaaagtgatg
     1921 aggatacgct tgagttatta aggtttagat attgggattg tcctattggt tgttatgaat
     1981 gggaagatat agcacattac tttggtacaa gtaagacaag tatattacgt agaaggaatg
     2041 cactgatcga taagttagca aagtatattg gttatgtgta gcggactttt accctatgta
     2101 agtccgcatt aaaacagttt attatgttag tatcagatta atatttaaag ttattaaatg
     2161 ctaatacgac gcatgaacaa gaggcgcatc actatgtgat gtgtattttt atttatgagg
     2221 tatgaacatg ttcaaactaa tagtaaatac attactacac atcaagtata gatgcgtctt
     2281 gatactactt aagttatata aggtgaaata ttatgatgac taaagacgaa cgcatacgat
     2341 tctataagtc taaagaatgg caaacaacaa gaaaaagagt gctagaaaga gataattatg
     2401 aatgtcaaca atgtaagaga gacggcaagt taacgacata tgacaaaagc aagcgtaagt
     2461 cgttggatgt agatcatata ttatcgctag aacatcatcc ggagtttgct catgacttaa
     2521 acaatttaga aacactgtgt attaaatgtc acaacaaaaa agaaaagaga tttataaaaa
     2581 aagaaaataa atggaaagac gaaaaatggt aaataccccc gggtcaaaaa aatcaaaagc
     2641 gatcaaaata cttggggaac ggggaggggc tcgacttcgc gataatttta aaaatccatg
     2701 tatacccccc ctcttataac cattttaagg caggtgatga aatggagatt atagttgatg
     2761 aaaacttagt gcttaaagaa aaagaaaggc tgcaagtatt atataaagac atacctagca
     2821 ataaattaaa agtagttgat ggtttaatta ttcaagcagc aaggctacgt gtaatgcttg
     2881 attacatgtg ggaagacata aaagaaaaag gtgactatga tttatttact caatctgaaa
     2941 aggcgccacc atatgaaagg gaaagaccag tagccaaact atttaatgct agagatgctg
     3001 catatcaaaa aataatcaaa caattatcgg atttattgcc cgaagagaaa gaagacacag
     3061 aaacgccatc tgatgattac ctatgattag taataaatac gttgatgaat atataaattt
     3121 gtggaaacaa ggaaagataa ttttaaataa agaaagaatt gatctcttta attatctaca
     3181 aaaacatata tattcacgag atgatgtata ttttgatgaa cagaaaatcg aggattgtat
     3241 caaatttatt gaaaaatggt attttccaac attaccattt caaaggttta tcatagctaa
     3301 tatatttctt atagataaaa atacagatga agctttcttt acagaatttg ctattttcat
     3361 gggacgtgga ggcgggaaaa acggtctaat aagtgctatt agtgattttc tttctacgcc
     3421 cttacacgga gttaaagaat atcacatctc cattgttgct aatagtgaag atcaagcaaa
     3481 aacatcgttt gatgaaatca gaaccgtttt aatggataac aaacgaaata agacgggtaa
     3541 aacgccaaaa gctccttatg aagttagtaa agcaaaaata ataaaccgtg caactaaatc
     3601 ggttattcga tataacacat caaacacaaa aaccaaagac ggtggacgtg aggggtgtgt
     3661 tatttttgat gaaattcatt atttctttgg tcctgaaatg gtaaacgtca aacgtggtgg
     3721 attaggtaaa aagaaaaata gaagaacgtt ttatataagt actgatggtt ttgttagaga
     3781 gggttatatc gatgcaatga agcacaaaat tgcaagtgta ttaagtggca aggttaaaaa
     3841 tagtagattg tttgcttttt attgtaagtt agacgatcca aaagaagttg atgacagaca
     3901 gacgtgggaa aaggcgaacc caatgttaca taaaccgtta tcagaatacg ctaaaacact
     3961 gctaagtacg attgaagaag aatataacga tttaccattc aaccgttcaa ataagcccga
     4021 attcatgact aagcgaatga atttgcctga agttgacctt gaaaaagtaa tagcaccatg
     4081 gaaagaaata ctagcgacta atagagagat accaaattta gataatcaaa tgtgtattgg
     4141 tggtttagac tttgcaaaca ttcgagattt tgcaagtgta gggctattat tccgaaaaaa
     4201 cgatgattac atttggttag gacattcgtt tgtaagacaa gggtttttgg atgatgtcaa
     4261 attagaacct cctattaaag aatgggaaaa aatgggatta ttgaccattg tcgatgatga
     4321 tgtcattgaa attgaatata tagttgattg gtttttaaag gctagagaaa aatatgggct
     4381 tgaaaaagtc atagctgata attatagaac tgatattgta agacgtgcgt ttgaggatgc
     4441 tggcataaaa cttgaagtac ttagaaatcc aaaagcaata catggattac ttgcaccacg
     4501 tatcgataca atgtttgcga aacataacgt aatatatgga gacaatcctt tgatgcgttg
     4561 gtttactaat aatgttgcag taaaggttaa acccgatggt aataaagaat atattaaaaa
     4621 agatgaaaat agaagaaaaa ccgatgggtt catggctttt gttcacgcat tatatagagc
     4681 agacgatata gtagacaaag acatgtctaa agcgcttgat gcattaatga gtatagattt
     4741 ctaatagagg aggtgagaca tgagtattct agaaaagata tttaaaacta ggaaagatat
     4801 aacatatatg cttgatttag atatgataga agatctatca caacaagcgt atgtgaaacg
     4861 tttagcgatt gatagttgta ttgaatttgt tgcgcgagct gtcgctcaaa gtcattttaa
     4921 agtattggaa ggtaatagaa ttcaaaagaa tgatgtttac tacaagttaa atataaaacc
     4981 aaatactgac ttatcaagcg atagtttttg gcaacaagtt atatataaac taatttatga
     5041 taacgaggtt ttaatcgtag taagtgacag caaagaatta cttatcgcag atagctttta
     5101 cagagaagag tacgctttgt atgatgatat attcaaagat gtaacggtta aagattatac
     5161 ttatcaacgt actttcacaa tgcaagaggt catatattta aagtacaaca acaataaagt
     5221 gacacacttt gtagaaagtc tattcgaaga ttacgggaaa atattcggaa gaatgatagg
     5281 tgcacaatta aaaaactatc aaataagagg gattttgaaa tctgcctcta gcgcatatga
     5341 cgaaaagaat atagaaaaat tacaagcgtt cacaaataaa ttattcaata cttttaataa
     5401 aaatcaacta gcaatcgcgc ctttgataga aggttttgat tatgaggaat tatctaatgg
     5461 tggtaagaat agtaacatgc ctttttctga attgagtgag ctaatgagag atgcaataaa
     5521 aaatgttgcg ttgatgattg gtatacctcc aggtttgatt tacggagaaa cagctgattt
     5581 ggaaaagaac acgcttgtat ttgagaagtt ctgtttaaca cctttattaa aaaagattca
     5641 gaacgaatta aacgcgaaac tcataacaca aagcatgtat ttgaaagata caagaataga
     5701 aattgtcggt gtgaataaaa aagacccact tcaatatgct gaagcaattg acaaacttgt
     5761 aagttctggt tcatttacaa ggaatgaggt gcggattatg ttaggtgaag aaccatcaga
     5821 caatcctgaa ttagacgaat acctgattac taaaaactac gaaaaagcta acagtggtga
     5881 aaatgatgaa aaagaaaaag atgaaaacac tttgaaaggt ggtgatgaag atgaaagcgg
     5941 agattaaagg cgtcatcgtt tccaacgaag ataaatgggt ttacgaaatg cttggtatgg
     6001 attcgacttg tcctaaagat gttttaacac aactagaatt tagtgatgaa gatgttgata
     6061 ttataattaa ctcaaatggt ggtaacctag tagctggtag tgaaatatat acacatttaa
     6121 gagctcataa aggcaaagtg aatgttcgca tcacagcaat agcagcaagt gcggcatcgc
     6181 ttatcgcaat ggctggtgac cacatcgaaa tgagtccggt tgctagaatg atgattcaca
     6241 atccttcaag tattgcgcaa ggagaagcga aagatctaaa tcatgctgca gaaacattag
     6301 aacatgttgg tcaaataatg gctgaggcat atgcggttag agctggtaaa aacaaacaag
     6361 aacttgtaga aatgatggct agggaaacgt ggctaaatgc tgatgaagcc attgaacaag
     6421 gttttgcgga tagtaaaatg tttgaaaacg acaatatgca aattgtagca agcaatacac
     6481 aagtgttatc gaaagatgta ttaaatcgtg taacagcttt ggtaagtaaa acgccagagg
     6541 ttaacattga tattgacgca atagcaaata aagtaattga aaaaataaat atgaaagaaa
     6601 aggaatcaga aatcgatgtt gcagatagta aagtatcagc aaatggattt tcaagattcc
     6661 ttttttaata caaaaaatag gaggtcataa aatgactata aatttatcgg aaacattcgc
     6721 aaatgcgaaa aacgaattta ttaatgcagt aaacaacggt gaaccgcaag aaagacaaaa
     6781 tgaattgtac ggtgacatga ttaaccaact atttgaagaa actaaattac aagcaaaagc
     6841 agaagctgaa agagtttcta gtttacctaa atcagcacaa tctttgagtg caaaccaaag
     6901 aagtttcttc atggatatca ataaaaacgt taactataaa gaagaaaaac ttttgccaga
     6961 agaaacaatt gatagaattt ttgaagattt gacgacgaat catccgttat tagctgattt
     7021 aggtattaaa aacgctggtt tgcgtttgaa gttcttaaaa tctgaaactt ctggcgtagc
     7081 cgtttggggt aaaatctatg gtgaaattaa aggtcaatta gatgctgcgt tcagtgaaga
     7141 aacagcaatt caaaataaat tgacagcgtt tgttgtttta ccaaaagatt taaatgattt
     7201 tggtcctgcg tggattgaaa gatttgttcg tgttcaaatc gaagaagcat ttgcagtggc
     7261 gcttgaaact gcgttcttaa aaggtactgg taaagaccaa ccaatcggct taaaccgtca
     7321 agtacaaaaa ggtgtatcgg taactgaggg tgcttatcca gagaaagaag aacaaggtac
     7381 gcttacattt gctaatccgc gcgctacagt taatgaattg acgcaagtgt ttaaatacca
     7441 ctcaactaac gagaaaggta aatcagtagc ggttaaaggt aatgtaacaa tggttgttaa
     7501 tccgtccgat gcttttgagg ttcaagcaca gtatacacat ttaaatgcaa atggcgtata
     7561 tgttactgct ttaccattta atttgaatgt tatcgagtct acagtccaag aagcaggtaa
     7621 ggttttaacg tacgttaaag gtttatatga tggttattta gctggtggta ttaatgttca
     7681 gaaatttaaa gaaacacttg cgttagatga tatggattta tacactgcaa aacaatttgc
     7741 ttacggcaaa gcgaaagata ataaagttgc tgctgtttgg aaattagatt taaaaggaca
     7801 taagccagct ttagaaggta ccgaagaaac actataaaat tttatgaggt gataaaatgg
     7861 tgaaatttaa agttgttaga gcttttaaag acatagagca caatcaacac aagtacaaag
     7921 taggggagtt gtatccagct gaagggtata acaatcctcg tgttgaattg ttgacaaatc
     7981 aaatcaaaaa taagtacgac aaagtttata tcgtaccttt agataagctg acaaaacaag
     8041 aattattaga actatgcgaa tcattacaaa aaaaagcgtc tagttcaatg gttaaaagtg
     8101 aaatcgtcga cttattgaat ggtgaagaca atgacgattg atgatttgct tgtcaaattt
     8161 aaatcacttg aaaagattga ccataattca gaggatgagt acttaaagca gttgttaaaa
     8221 atgtcgtacg agcgtataaa aaatcagtgc ggagtttttg aattagagaa tttaataggt
     8281 caagaattga tacttatacg cgctagatat gcttatcaag atttattaga acacttcaac
     8341 gataattaca gacctgaaat aatagatttt tcgttatctc taatggaggt atcagaagat
     8401 gaagaaagtg tttaaaaaac ctagaattac aactaaacgt ttaaatactc gtgttcattt
     8461 ttataagtat actgaaaata atggtccaga agctggagaa aaagaagaaa aattattata
     8521 tagctgttgg gcgagtattg atggtgtctg gttacgtgaa ttagaacaag ctatctcaaa
     8581 cggaacccaa aatgacatta aattgtatat tcgtgatccg caaggtgatt atttacccag
     8641 tgaagaacat tatcttgaaa ttgaatcaag atatttcaaa aatcgtttga atataaagca
     8701 agtatcacca gatttggata ataaagactt tattatgatt cgtggaggat atagttcatg
     8761 agtgtgaaag tgataggtga taaagcatta gaaagagaat tagaaaatcg tttcggaact
     8821 caagcgatgt taaaagttca agatagggcg ctaattgctg gtgctaaagt aattgttgaa
     8881 gaagtaaaaa aacaactaaa gccatcaaaa gatacgggcg cattaattaa tgaggtaagt
     8941 tttagtaaac ctgaatggat aaacggaaaa cgtacaatta ctgttcattg gcgaggttct
     9001 aaagaccgtt ataaaatcgt acatttaatt gaaaatggac acgttcaaaa agggacaggt
     9061 aaatttatca aacctaaagc tatgggtggc gttaatagag caataagaca agggcaaaat
     9121 aagtattttg agacgctaaa aagggagtta aaaaaattgt gattgatatt ttgtacaaag
     9181 ttcatgaagt gattagtcaa gacagaatta ttagagagca cgtaaatatc aataatatta
     9241 agttcaataa atacccaaac gtaaaagata cggatgtacc tttcatagtt attgacgata
     9301 tagacgaccc aatacctaca acttatactg acggagatga gcgcgcatat agttacattg
     9361 tacaaataga tgtatttgtg aagtttaacg aaaagtataa cgcgagaatc ataagaaata
     9421 agatatctaa tcgtattcaa aagttattat ggtctgaact gaaaatggga aatgtatcca
     9481 acggaaaacc tgaatatata gaagaattta aaacatatag aagcactcgt gtctacgagg
     9541 gcatttttta cgaggaggaa taataaatgg tagtaaaaca agcaagtgcg ccgaaagcgt
     9601 attttaacat tactggttta ggtttcgcta aattaacaaa agaaggcgca aagctagaat
     9661 atagcgatat tacaaaaact agaggattac aaaaaatcgg tgttgaaact ggaggagact
     9721 tgaaaacagc atatgctgat ggtggtccaa tcgaatcagg gaacacagac ggagaaggta
     9781 aaatttcgtt acaaatgcat gctttcccta aagagattcg caaaatagta ttcaatgaag
     9841 agtatgatga atacggtgtt tataaagaaa ctcaaggtaa acaaaacaac tatgtagcaa
     9901 tttggttcag acaagagcgt cgagacggca catttagaac ggttttatta cctaaagtca
     9961 tgtttacaaa tcctaaaatc gatggcgaaa cggctgagaa agattgggat ttctcaagcg
    10021 aagaggttga aggtgaggca cttttccctt taattgataa taaaaagtca gtacgtaaat
    10081 atatctttga ttcagcaaac atgacaaatc atagtggaaa tggtgaaaaa ggcgaagagg
    10141 ctttcttaaa gaaaatttta ggcgaagaat atactggaaa cgtgacagag gataacggcg
    10201 aaactttgta acgcaaccgg cttcatcgga aactgagata aagtcggtta atataccaga
    10261 tagttttaaa acactaaaag ttggcaacac atatgattta aatgttgtag tagagccatc
    10321 taatcaaagt aagttattga aatacacaac agatcaaacg aatattgtat caatcaatag
    10381 tgatggtcaa gtcactgcgg aagcacaagg tattgccacg gttaaagcat atgttggtaa
    10441 tatgagtgac actataacaa taaatgtaga agcataagag gggacaaccc ctctatttta
    10501 tttgaaaata aggagagtat tataaaatgg caaaattaaa acgtaacatt attcaattag
    10561 tagaagatcc aaaagcaaat gaaattaaat tacaaacgta cttaacacca cacttcattt
    10621 catttgaaat tgtatacgaa gcaatggatt taatcgatga tattgaggac gaaaatagca
    10681 cgatgaagcc aagagaaatc gctgacagat tgatggatat ggttgtaaaa atttacgata
    10741 accaattcgc agttaaagac ctaaaagaac gtatgcatgc acctgatgga atgaatgcac
    10801 ttcgtgaaca agtggttttc attacgcaag gtcaacaaac tgaggaaact agaaatttta
    10861 tccagaacat gaaataaagc ctgaagattt aacatataaa gcaatgttga aaaatatgga
    10921 tactctcatg atggacttaa ttgaaaatgg taaagacgct aacgaagttt taaaaatgcc
    10981 atttcattat gtgctttcca tatatcaaaa taaaaacaat gacatttctg aagaaaaagc
    11041 agaggcttta attgatgcat tttaacctta accgtttggt tagggttatt tttttgaact
    11101 tttttagaaa ggaggtaaaa aatgggagaa agaataaaag gtttatctat aggtttggat
    11161 ttagatgcag caaatttaaa tagatcattt gcagaaatca aacgaaactt taaaacttta
    11221 aattctgact taaaattaac aggcaacaac ttcaaatata ccgaaaaatc aactgatagt
    11281 tacaaacaaa ggattaaaga acttgatgga actatcacag gttataagaa aaacgttgat
    11341 gatttagcta agcaatatga caaggtatct caagaacagg gcgaaaacag tgcaggagct
    11401 caaaagttac gacaagaata taacaaacaa gcaaatgaac tgaattattt agaaagagaa
    11461 ttacaaaaaa catcagccga atttgaagag ttcaaaaaag ctcaagttga agctcaaaga
    11521 atggcagaaa gtggctgggg aaaaaccagt aaagtttttg aaagtatggg acctaaatta
    11581 acaaaaatgg gtgatggttt aaaatccatt ggtaaaggca tgatgattgg tgtaactgca
    11641 cctgttttag gtatcgcagc agcatcagga aaagcttttg cagaagttga taaaggttta
    11701 gatactgtta ctcaagcaac aggcgcaaca ggcagtgaat taaaaaaatt gcagaactca
    11761 tttaaagatg tttatggcaa ttttccagca gatgctgaaa ctgttggtgg agttttagga
    11821 gaagttaata caagattagg ttttacaggt aaagaacttg aaaatgccac agagtcattc
    11881 ttgaaattca gtcatataac aggttctgac ggtgtgcaag ccgtacagtt aattacccgt
    11941 gcaatgggcg atgcaggtat cgaagcaagt gaatatcaaa gtgttttgga tatggtagca
    12001 aaagcggcgc aagctagtgg gataagtgtt gacacattag ctgatagtat tactaaatac
    12061 ggcgctccaa tgagagctat gggctttgag atgaaagaat caattgcttt attctctcaa
    12121 tgggaaaagt caggcgttaa tactgaaata gcattcagtg gtttgaaaaa agctatatca
    12181 aattggggta aagctggtaa aaacccaaga gaagaattta agaagacatt agcagaaatt
    12241 gaaaagacgc cggatatagc tagcgcaact agtttagcga ttgaagcatt tggtgcaaag
    12301 gcaggtcctg atttagcaga cgctattaaa ggtggtcgct ttagttatca agaattttta
    12361 aaaactattg aagattccca aggcacagta aaccaaacat ttaaagattc tgaaagtggc
    12421 tccgaaagat ttaaagtagc aatgaataaa ttaaaattag taggtgctga tgtatgggct
    12481 tctattgaaa gtgcgtttgc tccagtcatg gaagaattaa taaaaaagct atcagtcgct
    12541 gtcgattggt tttcgaattt aagtgatggt tctaaaagat caattgttat tttcggtggt
    12601 attgctgctg caattggtcc tgtagttttt gggttaggtg catttataag cacaattggt
    12661 aatgcagtaa ctgtattagc cccactatta gctggtattg caaaggctga tggattaatt
    12721 agttttttat cgactaaagt acctatatta ggaactgtct tcacagcttt aactggtcca
    12781 attggcattg tattaggtgt tttagctggc ctagcagtcg catttacaat tgcttataag
    12841 aaatctgaaa catttagaaa ttttgttaat gatgcaattg aaagtgttaa acaaacattt
    12901 agtaatttta ttcaatttat tcaacctttc attgattccg ttaaaaacgt ctttaaacaa
    12961 gcggtttcag caatcgttga tttcgctaaa gatatttgga gtcaaattaa tggattcttt
    13021 aatgaaaacg gaatttctat tgttcaagcg cttcaaaata tatgcaattt tatcaaagtt
    13081 atatttgaat ttatcttaaa ttttgtaatt aaaccaataa tgtttgcaat ctggcaagtg
    13141 atgcaattta tttggccggc ggttaaagcc ttgattgtca gtacttggga gaatataaaa
    13201 ggtgtgatac aaggaacttt aaatatcata ctaggtttaa ttaagttctt ctcaagttta
    13261 tttactggag attggcgagg agtttgggac gcgattatta tgattcttaa aggagtcgtt
    13321 caattaatat ggaatttaat tcaattatgg tttgtaggca aaatacttgg cgttgttagg
    13381 tactttggcg gattgctaaa aggattaata acaggtattt gggacgtaat aaaaagtata
    13441 ttcagtaaat ctttatcagc aatttggaat gcgacaaaaa gtatttttgg attcttattt
    13501 aatagtgtca aatcaatttt cacgaatatg aaaaattggt tatctaacac atggagtagt
    13561 atccgtacga atacgatagg aaaagcacag tcattattta gtggtgtgaa atcaaaattc
    13621 actagtttgt ggaatgcgac aaaagatatt tttagtaatc taagaaattg gatggcaaat
    13681 atttggaatt ccattaaaga taatacggtt ggaattgcaa gtcgtttatg gagtaaagtg
    13741 cgtggaattt ttacaaatat gcgtgacggc ttacaaagta ttatcagcaa aattaaaagt
    13801 catatcggcg gtatggtaga tgctattaaa aaaggactta ataaattaat tgaaggttta
    13861 aactgggtcg gtggtaagct gggaatggat aaaataccta agttacacac tggtacagaa
    13921 cacacacata ctactacaag attagttaag aacggtaaga ttgcgcggga tacgttcgct
    13981 acggttgggg ataaaggacg tggaaatggt ccgaatggtt tcagaaatga aataattgaa
    14041 ttccctaatg gtaaacgtgt aatcacacct aatacagata ctaccgctta tttacctaaa
    14101 ggctcaaaag tatacaacgg tacacaaact tattcaatgt taaacggaac gcttccaaga
    14161 tttagcttag gtactatgtg gaaagatatt aaatccggtg catcatcggc atttaactgg
    14221 acaaaagatc aaataggtaa aggtaccaaa tggcttggcg ataaagttgg cgatgtttta
    14281 gattttatgg aaaatcctgg caaactttta aattatatac ttgaagcttt tggaattgat
    14341 ttcaattctt taactaaagg tatgggaatt gcaggcgaca taacaaaagc tgcatggtct
    14401 aagattaaga aaagtgctac tgattggata aaagaaaatt tagaagctat gggcggtggc
    14461 gatttagtcg gcggaatatt agaccctgac aaaattaatt atcattatgg acgtaccgca
    14521 gcttatactg ctgcaactgg aagaccattt catgaaggtg tcgattttcc atttgtatat
    14581 caagaagtta gaacgccgat gggtggcaga cttacaagaa tgccatttat gtctggtggt
    14641 tatggtaatt atgtaaaaat tactagtgga gttatcgata tgctatttgc gcatttgaaa
    14701 aactttagca aatcaccacc tagtggcacg atggtaaagc ccggcgatgt tgttggttta
    14761 actggtaata ccggatttag tacaggacca cacttacatt ttgaaatgag aagaaatgga
    14821 cgtcactttg accctgaacc atatttaaga aatgcaaaga aaaaaggtag gttatcaatt
    14881 ggtggcggtg gtgctacttc tggaagtggt gcaacttatg caagccgagt aatccgtcaa
    14941 gcgcaaagta ttttaggagg acgttataaa ggtaagtgga ttcatgacca gatgatgcga
    15001 gttgcaaagc gtgaaagtaa ctatcaatca aatgcagtga ataattggga tattaatgct
    15061 caaagaggag acccatcaag aggattattc caaatcatcg gctcaacttt tagagcaaac
    15121 gctaaacgtg gatatactaa ctttaataat ccggttcatc aaggtatttc agcaatgcag
    15181 tacattgtta gacgctacgg ttgggatgga tttaagcgtg ctggtgatta cgcatatgct
    15241 acaggtggaa aagtttttga tggttggtat aacttaggtg aagacggtca tccagaatgg
    15301 attattccaa cagatccagc tcgtagaaat gatgcaatga agatgttgca ttatgcagca
    15361 gcagaagtaa gagggagaaa agcgagtaaa aataagcgtc ctagtcaatt gtctagtgta
    15421 aatgggtttg atgacccaag cttattattg aaaatgattg aacaacagca acaacaaata
    15481 gctttattac tgaaaatagc gcaatccaac gatgtgattg cagataaaga ttatcagccg
    15541 attattgacg aatacgcttt tgataaaaag gtgaacgcgt ctatagaaaa gcgagaaagg
    15601 caagaatcaa caaaagtaaa gtttagaaaa ggaggaattg ctattcaatg atagacacta
    15661 ttaaagtgaa caacaaaaca attccttggt tgtatgtcga aagagggttt gaaataccct
    15721 cttttaatta tgttttaaaa actgaaaatg tagatggacg ttcggggtct atatataaag
    15781 ggcgtaggct tgaatcttat agttttgata tacctttggt ggtacgtaat gactatttat
    15841 ctcacaacgg ctttaaaaca catgatgacg tcttgaatga attagtaaag ttttttaact
    15901 acgaggaaca agttaaatta caattcaaat ctaaagattg gtactggaac gcttattttg
    15961 aaggaccaat aaagctgcac aaagaatttg caatacctgt taagttcact atcaaagtag
    16021 tactaacaga cccttacaaa tattcagtaa caggatataa aaatactgcg atttcagacc
    16081 aagtttcagt tgtaaatagt gggactgctg acactccttt aattgttgaa gcccgagcaa
    16141 ttaaaccatc tagttacttt atgatcacta aaaatgatga agattatttt atggttggtg
    16201 atgatgaggt aaccaaagaa gttaaggatt acatgcctcc tgtttatcat agtgagtttc
    16261 gtgatttcaa aggttggact aagatgatta ctgaagatat tccaagtaat gatttaggtg
    16321 gtaaagtcgg cggtgacttt gtgatatcca atcttggcga aggatataaa gcaactaatt
    16381 tttctgatgc aaaaggttgg gttggtgctg gcacgaaacg agggctccct aaagcgatga
    16441 cagattttca aattacctat aaatgtattg ttgaacaaaa aggtaaaggt gccggaagaa
    16501 cagcacaaca tatttatgat agtgatggta agttacttgc ttctattggt tatgaaaata
    16561 aatatcatga tagaaaaata ggacatattg ttgttacgtt gtataaccaa aaaggagacc
    16621 ccaaaaagat atacgactat cagaataaac cgataatgta taacttggac agaatcgttg
    16681 tttatatgcg gctcagaaga gtaggtaata aattttctat taaaacttgg aaatttgatc
    16741 acattaaaga cccagataga cgtaaaccta ttgatatgga tgagaaagag tggatagatg
    16801 gcggtaagtt ttatcagcgt ccagcttcta tcatagctat ctatagtgcg aagtataatg
    16861 gttataagtg gatggagatg aatggattag gttcattcaa tacggagatt ctaccaaaac
    16921 cgaaaggcgc aagggatgtc attatacaaa aaggtgattt agtaaaaata gatatgcaag
    16981 caaaaagtgt tgtcatcaat gaggaaccaa tgttgagcga gaaatcgttt ggaagtaatt
    17041 atttcaatgt tgattctggg tacagtgaat taatcataca acctgaaaac gtctttgata
    17101 cgacggttaa atggcaagat agatatttat agaaaggaga tgagagtgtg atacatgttt
    17161 tagattttaa cgacaagatt atagatttcc tttctactga tgacccttcc ttagttagag
    17221 cgattcataa acgtaatgtt aatgacaatt cagaaatgct tgaactgctc atatcatcag
    17281 aaagagctga aaagttccgt gaacgacatc gtgttattat aagggattca aacaaacaat
    17341 ggcgtgaatt tattattaac tgggttcaag atacgatgga cggctacaca gagatagaat
    17401 gtatagcgtc ttatcttgct gatataacaa cagctaaacc gtatgcacca ggcaaatttg
    17461 agaaaaagac aacttcagaa gcattgaaag atgtgttgag cgatacaggt tgggaagttt
    17521 ctgaacaaac cgaatacgat ggcttacgta ctacgtcatg gacttcttat caaactagat
    17581 atgaagtttt aaagcaatta tgtacaacct ataaaatggt attggatttt tatatagagc
    17641 ttagttctaa taccgtcaaa ggtagatatg tggtactcaa aaagaaaaac agcttattca
    17701 aaggtaaaga aattgagtat ggtaaagatt tggttgggtt aactaggaag attgatatgt
    17761 cagaaatcaa aacagcatta attgctgtgg gacccgaaaa tgacaaagga aagcgtttag
    17821 agctagttgt gacagatgac gaagcgcaaa gtcaattcaa cctacctatg cgctatattt
    17881 gggggatata tgaaccacaa tcagatgatc aaaatatgaa tgaaacacga ttaagttctt
    17941 tagccaaaac agagttaaat aaacgtaagt cggcagttat gtcatatgag attacttcta
    18001 ctgatttgga agttacgtat ccgcacgaga ttatatcaat tggcgataca gtcagagtaa
    18061 aacatagaga ttttaacccg ccattgtatg tagaggcaga agttattgct gaagaatata
    18121 acataatttc agaaaatagc acatatacat tcggtcaacc taaagagttc aaagaatcag
    18181 aattacgaga agagtttaac aagcgattaa acctaataca ccaaaaatta aacgacaata
    18241 ttagcaatat caatactata gtaaaagatg ttgtagatgg tgaattagaa tactttgaac
    18301 gcaaaattca taaaagtgat acaccgccag aaaatccagt caatgatacg ctttggtatg
    18361 atacaagtaa ccctgatgtt gctgtcttgc gtagatattg gaatggtcga tggattgaag
    18421 caacaccaaa tgatgttgaa aaattaggtg gtataacaag agagaaagcg ctattcagtg
    18481 aattaaacaa tatttttatt aatttatcta tacaacacgc tagtcttttg tcagaagcta
    18541 cagaattact gaatagcgag tacttagtag ataatgattt gaaagcggac ttacaagcaa
    18601 gtttagacgc tgtgattgat gtttataatc aaattaaaaa taatttagaa tctatgacac
    18661 ccgaaactgc aacgattggt cggttggtag atacaaaaac tttatttctt gagtatagaa
    18721 agaaattaca agatgtttat acagatgtag aagatgtcaa aatcgccatt tcagatagat
    18781 ttaaattatt acagtcacaa tacactgatg aaaaatataa agaagcgttg gaaataatag
    18841 caacaaaatt tggtttaacg gtgaatgaag atttgcagtt agtcggagaa cctaatgttg
    18901 ttaaatcagc tattgaagca gctagagaat ccacaaaaga acaattacgt gactatgtaa
    18961 aaacatcgga ctataaaaca gacaaagacg gtattgttga acgtttagat actgctgaag
    19021 ctgagagaac gactttaaaa ggtgaaatca aagataaagt tacgttaaac gaatatcgaa
    19081 acggattgga agaacaaaaa caatatactg atgaccagtt aagtgatttg tccaataatc
    19141 ctgagattaa agcaagtatt gaacaagcaa atcaagaagc gcaagaagct ttaaaatcat
    19201 acattgatgc tcaagataat cttaaagaga aggaatcgca agcgtatgct gatggtaaaa
    19261 tttcggaaga agagcaacgc gctatacaag atgctcaagc taaacttgaa gaggcaaaac
    19321 aaaacgcaga actaaaggct agaaacgctg aaaagaaagc taatgcttat acagacaaca
    19381 aggtcaaaga aagcacagat gcacagagga gaacactgac tcgctatggt tctcaaatta
    19441 tacaaaatgg taaggaaatc aaattaagaa ctactaaaga agagtttaat gcaaccaatc
    19501 gtacactttc aaatatatta aacgagattg tccaaaacgt tacagatgga acaacaatca
    19561 gatatgatga taacggagtg gctcaagctt taaatgtggg gccacgtggt attagattaa
    19621 atgctgataa aattgatatt aacggtaata gagaaataaa ccttcttatc caaaatatgc
    19681 gagataaagt agataaaacc gatattgtca acagccttaa tttatcaaga gagggtcttg
    19741 atatcaatgt taatagaatt ggaattaaag gcggtaacaa taacagatat gttcaaatac
    19801 agaatgattc tattgaacta ggtggtattg tgcaacgaac ttggaaaggc aaacgatcaa
    19861 ccgatgatat attcacacgt cttaaagatg gacatctaag gtttagaaat aataccgcag
    19921 gcggttcact ttatatgtca cattttggta tttcaacata tattgatgga gaaggcgaag
    19981 acggaggttc atccggtact attcaatggt gggataaaac ttacagtgat agcggtatga
    20041 atggcataac aatcaattcc tatggtggtg tcgttgcact aacgtcagat aataatcggg
    20101 ttgttctgga gtcttacgct tcatcgaata tcaaaagcaa acaggcaccg gtgtatttat
    20161 atccaaacac agacaaagtg cctggattaa accgatttgc attcacgctg tctaatgcag
    20221 ataatgctta ttcgagtgac ggttatatta tgtttggttc tgatgagaac tatgattacg
    20281 gtgcgggtat caggttttct aaagaaagaa ataaaggtct tgttcaaatt gttaatggac
    20341 gatatgcaac aggtggagat acaacaatcg aagcagggta tggcaaattt aatatgctga
    20401 aacgacgtga tggtaatagg tatattcata tacagagtac agacctactg tctgtaggtt
    20461 cagatgatgc aggagatagg atagcttcta actcaattta tagacgtact tattcggccg
    20521 cagctaattt gcatattact tctgctggca caattgggcg ttcgacatca gcgcgtaaat
    20581 acaagttatc tatcgaaaat caatataacg atagagatga acaactggaa cattcaaaag
    20641 ctattcttaa cttacctatt agaacgtggt ttgataaagc tgagtctgaa attttagcta
    20701 gagagctgag agaagataga aaattatcgg aagacaccta taaacttgat agatacgtag
    20761 gtttgattgc tgaagaggtg gagaatttag gattaaaaga gtttgtcacg tatgatgaca
    20821 aaggagaaat tgaaggtata gcgtatgatc gtctatggat tcatcttatc cctgttatca
    20881 aagaacaaca actaagaatc aagaaattgg aggagtcaaa gaatgcagga taacaaacaa
    20941 ggattacaag ctaatcctga atatacaatt cattatttat cacaggaaat tatgaggtta
    21001 acacaagaaa acgcgatgtt aaaagcgtat atacaagaaa ataaagaaaa tcaacaatgt
    21061 gctgaggaag agtaatcttt agcactattt ttatacaaaa atttaaggag gtcatttaat
    21121 tatggcaaaa gaaattatca acaatacaga aaggtttatt ttagtacaaa tcgacaaaga
    21181 aggtacagaa cgtgtagtat atcaagattt cacaggaagt tttacaactt ctgaaatggt
    21241 taaccatgct caagatttta aatctgaaga aaacgctaag aaaattgcgg agacgttaaa
    21301 tttgttatat caattaacta acaaaaaaca acgtgtgaaa gtagttaaag aagtagttga
    21361 aagatcagat ttatctccag aggtaacagt taacactgaa acagtatgaa aagctatgag
    21421 ttagatactc ataatcttta ttcttttaga aagcgggtgt actgaattgg ggtggttcaa
    21481 aaaacacgaa catgaatggc gcatcagaag gttagaagag aatgataaaa caatgctcag
    21541 cacactcaat gaaattaaat taggtcaaaa aacccaagag caagttaaca ttaaattaga
    21601 taaaacctta gatgctattc aaaaagaaag agaaatagat gaaaagaata agaaagaaaa
    21661 tgataagaac atacgtgata tgaaaatgtg ggtgcttggt ttagttggga caatatttgg
    21721 atcgctaatt atagcattat tgcgtatgct tatgggcata taagagaggt gaataaaatg
    21781 tttaaactaa tctttggtta tagtttctgg acatgttttt ggttcggtaa atgtaaataa
    21841 gttttagtca gtgcttcggt actgactttt tatttattgt tgtaattatg gtaatatgca
    21901 gaagtgagca agttggatag atggtggcta tctgagtata aggaggtggt gcctatggtg
    21961 gcattactga aatctttaga aaggagacgc ctaatgatta caattagtac catgttgcag
    22021 tttggtttat tccttattgc attgataggt ctagtaatca agcttattga attaagcaat
    22081 aaaaaataac catcgctaac tttggctggt ttcgatggtt aaatggttat taatttaatc
    22141 tttaatctaa aatagccacc gtctttttaa cgggctcatt agggtaacat gtttgcgcat
    22201 gttgcccttt ttctatatat aaattaacac accataatat aaatatcaaa tagacggctt
    22261 attagtcgtc tttttatttt gggtaaaagg agataagaat atgattaatt ggaaaattag
    22321 aatgaaacaa aaatcatttt gggtagcgat attgtcagct atctttttat ttgctcaaaa
    22381 catcgcaaaa gctattgggt atgatatcca agtttataca gagcaattaa cagacggttt
    22441 aaacgctata ttaggatttt tagtattaac tggtgtgatt caagacccga ctactaaagg
    22501 tataggtgat agccaccaag ctttagaata tgaagaacca agaagaaaat actaggaggt
    22561 aaaataatga aaacatacag tgaagcaaga gcaaggttac gttggtatca aggtagatat
    22621 attgattttg acggttggta tggttaccaa tgtgcagatt tagcagttga ttacatttat
    22681 tggttgttag aaattagaat gtggggaaat gcaaaagatg caatcaataa cgattttaaa
    22741 aacatggcaa cagtatatga aaacacacca tcgtttgttc cacaaatagg tgatgtggct
    22801 gtatttacca aaggaatata taaacaatac ggtcatattg gtttagtgtt taatggtggt
    22861 aatacaaacc aatttttaat tttggaacag aactatgacg gtaacgcaaa tacgcctgca
    22921 aagttacgtt gggataatta ttacggctgt actcacttta ttagacctaa gtataaaagt
    22981 gagggcttaa tgaataagat cacaaataaa gttaaaccac ctgctcaaaa agcagtcggt
    23041 aaatctgcaa gtaaaataac agttggaagt aaagcgcctt ataaccttaa atggtcaaaa
    23101 ggtgcttatt ttaatgcgaa aatcgacggc ttaggtgcta cttcagccac tagatacggt
    23161 gataatcgta ctaactatag attcgatgtt ggacaggctg tatacgcgcc tggaacatta
    23221 atatatgtgt ttgaaattat agatggttgg tgtcgcattt attggaacaa tcataatgag
    23281 tggatatggc atgagagatt gattgtgaaa gaagtgtttt aattc
//
LOCUS       DALAAR010000041        22951 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000041 DALAAR010000000
VERSION     DALAAR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22951
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.043"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            27..1082
                     /locus_tag="O2458_001663"
     CDS             27..1082
                     /locus_tag="O2458_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCZ3887528.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            complement(1252..1893)
                     /locus_tag="O2458_001664"
     CDS             complement(1252..1893)
                     /locus_tag="O2458_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCZ3887529.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            2037..2903
                     /gene="fakB1"
                     /locus_tag="O2458_001665"
     CDS             2037..2903
                     /gene="fakB1"
                     /locus_tag="O2458_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCZ3887530.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3267..4349
                     /locus_tag="O2458_001666"
     CDS             3267..4349
                     /locus_tag="O2458_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ3887531.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            4342..5016
                     /locus_tag="O2458_001667"
     CDS             4342..5016
                     /locus_tag="O2458_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCZ3887532.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRSK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            5077..5649
                     /gene="raiA"
                     /locus_tag="O2458_001668"
     CDS             5077..5649
                     /gene="raiA"
                     /locus_tag="O2458_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCZ3887533.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            6063..8594
                     /gene="secA"
                     /locus_tag="O2458_001669"
     CDS             6063..8594
                     /gene="secA"
                     /locus_tag="O2458_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCZ3887534.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            8636..8740
                     /locus_tag="O2458_001670"
     CDS             8636..8740
                     /locus_tag="O2458_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887535.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            8909..10019
                     /gene="prfB"
                     /locus_tag="O2458_001671"
     CDS             join(8909..8980,8982..10019)
                     /gene="prfB"
                     /locus_tag="O2458_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCZ3887536.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            10173..10280
                     /locus_tag="O2458_001672"
     CDS             10173..10280
                     /locus_tag="O2458_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887537.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            10428..11267
                     /locus_tag="O2458_001673"
     CDS             10428..11267
                     /locus_tag="O2458_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887538.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            11441..12091
                     /locus_tag="O2458_001674"
     CDS             11441..12091
                     /locus_tag="O2458_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ3887539.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            12088..12324
                     /locus_tag="O2458_001675"
     CDS             12088..12324
                     /locus_tag="O2458_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCZ3887540.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            12593..14578
                     /gene="uvrB"
                     /locus_tag="O2458_001676"
     CDS             12593..14578
                     /gene="uvrB"
                     /locus_tag="O2458_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCZ3887541.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            14586..17432
                     /gene="uvrA"
                     /locus_tag="O2458_001677"
     CDS             14586..17432
                     /gene="uvrA"
                     /locus_tag="O2458_001677"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCZ3887542.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            18039..18971
                     /locus_tag="O2458_001678"
     CDS             18039..18971
                     /locus_tag="O2458_001678"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCZ3887543.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            18977..19816
                     /locus_tag="O2458_001679"
     CDS             18977..19816
                     /locus_tag="O2458_001679"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCZ3887544.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            19824..20309
                     /locus_tag="O2458_001680"
     CDS             19824..20309
                     /locus_tag="O2458_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ3887545.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            20317..21756
                     /locus_tag="O2458_001681"
     CDS             20317..21756
                     /locus_tag="O2458_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ3887546.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            21823..22758
                     /gene="trxB"
                     /locus_tag="O2458_001682"
     CDS             21823..22758
                     /gene="trxB"
                     /locus_tag="O2458_001682"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCZ3887547.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         8276 a         3153 c         4375 g         7147 t
ORIGIN      
        1 caattaatat cgatgaaggt gaataaatgg ttacattatt actagttgca gtaacaatga
       61 ttgtcagttt gacgataaca ccaattgtta ttgcaatatc gaaaagatta aatttagttg
      121 ataaaccaaa ttttagaaaa gtacacacta aacctatttc agttatgggt ggtacagtga
      181 ttctcttttc atttttaata ggtatttgga ttggtcatcc tattgaaaca gaaatcaaac
      241 cacttattat tggtgcgatt attatgtacg tacttgggct tgtagatgat atctacgatt
      301 tgaaaccgta tataaaattg gctggtcaaa ttgccgctgc cttagtagtt gctttttatg
      361 gtgtgactat tgattttatt tcgttgccaa tgggtacaac gattcatttt ggatttctta
      421 gtattccaat tactgtgatt tggattgttg ctattacaaa tgcaattaac ttaattgatg
      481 gactcgatgg tttggcgtcg ggtgtttctg caatcggact cattacaata gggttcattg
      541 caattttaca agctaatatt ttcataacga tgatttgttg tgttttatta ggctctttaa
      601 ttgggttttt attttacaat ttccatcctg ccaaaatatt tttaggtgat agtggggctt
      661 taatgattgg atttatcatc ggattccttt ctttactcgg attcaaaaat attacaatta
      721 ttgcattgtt cttcccaatt gttatcttag cagttccatt cattgatact ttgttcgcaa
      781 tgattcgacg tgtgaaaaaa gggcagcata taatgcaagc tgataaatcg catttgcatc
      841 ataaactatt agctttaggc tacacacata gacaaacagt attattaatc tattcaatct
      901 ctattttatt tagtctttcg agcattattt tgtatgtatc gccaccatta ggtgttgtat
      961 taatgtttgt attaatcata tttagtattg aattaattgt tgaatttaca ggattaatag
     1021 ataacaacta ccgaccaata ttaaatttaa ttagtcgtaa gtcatctcat aaagaggaat
     1081 agggaatgaa agcatagctg tatgggataa tttgtattat atggctttac tctttacaat
     1141 ttttttgtat taaatttcaa atataaaaag cactgccata aacgtgtact tcaattgtcg
     1201 tttaataata cgcaattgat atttaccgtc ttatgatagt gctttttatt tttattcagt
     1261 tggtatatcg aaaggtaact gctttggagt ttcttcagtc aaatcgaaat ttcctgcagt
     1321 catttgattt aaaaagttaa taaacgcttc atagtcactt ttaacgacat cgatatagta
     1381 gcttacctta tcagtgtaag tttggtttct taacataaaa tgagttgaag ctaattcata
     1441 ttcaaattta ccagtttgat cataattcag tgttactata catggtactg cttctcgtag
     1501 ttcgacacgc ccgatatcat aaatgacgtc tctaacagca ccgctatagg cgcgaattaa
     1561 accgccacca cctaatttaa taccaccaaa atatcttgtt actacgacac acgcattatg
     1621 aacatcgagc ttttttaata tgtctaacat tgggacaccg gcagttcctg tcggttcacc
     1681 atcatcattc gctttttgaa tattcatttc aggtccaata gtatatgcag aacaattatg
     1741 agtggcatct ttatgttctt tttttattgc agcaataaat gctttagctt catcttcatt
     1801 ttgaacaggt ttgatatgag caatgaatct tgatttacta atcacatttt caataatgtg
     1861 ttctttttta acagtaatga tattttgtgt cataataact ccttaattca taagcttaag
     1921 attatttaat cttcattata cactgaaaat gacatgacta taaatcgttt gattgccatt
     1981 ttctttttaa ctgaaatatt gtatcattgc tatgagtata ttttaggagg acgactatga
     2041 aaattgctgt gatgaccgat tctacaagtt atctgtcgca ggacttaatc gataaatata
     2101 atattcaaat agcgccatta agtgtgactt ttgatgatgg gaagaacttt acagaaagta
     2161 atgaaatagc aattgaagaa ttttataata aaatggcatc gtctcaaacg attccaacaa
     2221 caagccaacc agcaattggc gaatggatta ctaaatatga aatgttaaga gatcaaggtt
     2281 acacagatat cattgtcatt tgcttatcaa gtgggattag tggaagttat caatctagtt
     2341 atcaagcagg ggaaatggtt gaaggtgtta atgtacatgc atttgatagt aagcttgcag
     2401 caatgattga aggatgttat gtattacgtg ctattgaaat ggttgaagaa ggatacgagc
     2461 cacagcaaat tattgatgat ttaactaata tgcgtgaaca cacaggtgca tatttaattg
     2521 ttgatgactt aaagaattta caaaaaagtg gtcgaattac tggtgctcaa gcatgggttg
     2581 gaacattatt gaaaatgaag ccagttctta agtttgaaga tggcaagatt ataccagaag
     2641 aaaaagttcg tactaaaaag cgtgccattc aaacattaga aaagaaagta ttagatattg
     2701 taaaagactt tgaagaagta actttatttg tcataaatgg agatcatttc gaagatggtc
     2761 aagcgttata caaaaagtta caagatgatt gtccttcagc ttatcaagta gcatactctg
     2821 agtttggtcc agttgttgca gcacatttag gttctggtgg attaggttta ggctatgttg
     2881 gcagaaaaat aagattaaca taattataaa attttaataa aagagtctat attgtaattg
     2941 gaaattatct ctcgtataca tggctttaaa tgttcatcat ttgaaagcca aaatgctaaa
     3001 gatataagaa aatcattata atattaggct cttttttacg ttgaaatgag gttttaagca
     3061 ttaaacatta cgggaaatta attcatcctc atacttcact tactaatgaa aaaattaaaa
     3121 aagaagtaac aggtgtcatc aaacaaaatt caaactatta ttgtgttcaa tgtgaaagta
     3181 caaatccaaa gcatttttat cagtatgatt cctcagtaca ttccaagaaa attgtatatt
     3241 gcagaaattg tatatcactg ggtcgaatgg ataatgtaac aagatataaa ataacagaga
     3301 gttcgcaaag ttcatcacaa gcatattatc atctctcatt tgaattgtcg gaacagcagt
     3361 cttatgcctc agaacatatt gttcgagcca ttagaaagag acaaacgatt ttgttatatg
     3421 ccgtaacagg tgcaggtaag acagaaatga tgtttcaagg cattcaatat gcaagaatac
     3481 agggagataa tatagctatt gtgtcaccac gtgtagatgt tgttgtagaa attagtaaac
     3541 gtattaaaga cgcatttctt aatgaagata tagacatact acaccagcaa tcaagacaac
     3601 aatttgaagg gcattttgtt gtatgcacag tgcatcaact ttaccgattc aaacagcact
     3661 ttgatactat ttttattgat gaagtcgatg cctttccttt atcaatggat aaaaatttac
     3721 aacaagcatt gaagtcatct tctaaagttg aacatgcaac aatttatatg acagcaacac
     3781 caccgaaaca acttctgtca gagattcccc acgaaaatat aattaaattg ccagctcgct
     3841 ttcataaaaa atcacttcca gttcctaaat atcgttattt caaacttaat aataagaaga
     3901 ttcagaaaat gttataccga attttacaag atcaaattaa taatcaacgt tatacactgg
     3961 tgttttttaa caatatagaa acaatgatta aaacattttc ggtttataag cagaaaatta
     4021 ctaaattaac atacgtccat agcgaggatg tttttcgctt tgaaaaagtt gaacaattaa
     4081 ggaatggaca tttcgatgtc atttttacta cgacaatatt agaacgtgga tttacaatgg
     4141 caaatttgga tgttgttgtt atcgatgcac atcaatatac tcaagaggct ttaatacaaa
     4201 ttgctggacg tgttggacga aaattagaat gtcctactgg aaaagtattg ttttttcatg
     4261 aaggggtaag tatgaatatg attcaagcta aaaaagagat tcaaaggatg aacaaattag
     4321 cattaaaaag aggttggatt gatgaataat tgtttgagtt gtggtgctaa gttatatgaa
     4381 aatataacca tttataattt gttcaagaaa cctaatagat tatgtgacag atgcaaagag
     4441 aattgggaca atattaaact tgatattaaa gcaaggcgat gttcaaggtg cttaaaacac
     4501 ttaaatcaag atgaagcgta ttgtttagac tgcaagtttc tatcggcaca ctttaattta
     4561 atggaacaat tatattgtca atttcaatat gacggtttaa tgaaagagat gatacatcag
     4621 tataaatttt tgaaagacta ttatttatgt gaattattgg cacatttgat tgaaatacca
     4681 caaacatctt atgactatat tgtgccaatt ccttcttcgc cggcacatga tttatctaga
     4741 acatttaacc cggtagaagc agtactaaaa gctaaaggga ttcgctttga taagatttta
     4801 aagatgtcaa atagatcaaa acagtctcat ttaactaaga aagagcgtct ggcagatgaa
     4861 aatccattta ttattgatac ggaattagat ttaaatggta aggaaatatt actcgttgac
     4921 gatatttata caactggatt aacaattcat cgtgcagggt gtaaattata tgctaaaaat
     4981 atcagaaaat tcaaagtgtt tgcgtttgca cgatagcgta aaaatgttaa aatataataa
     5041 agagttacca ataaagaggt ttaaggagag attactatga ttagatttga aattcatgga
     5101 gataacctca ctatcacaga tgctattcgc aactatattg aggaaaaaat tggtaagttg
     5161 gaacgttatt ttaatgacgt accaaatgca gtggcgcatg ttaaagttaa aacttattca
     5221 aattcagcta ctaaaattga agtaacaatt ccattgaaaa atgttacgtt aagagctgaa
     5281 gagcgaaacg atgatttata cgcaggtatt gatttaatta ataataaact tgaaagacaa
     5341 gttcgaaaat ataaaacacg tattaatcgt aagagccgtg atcgaggaga tcaagaagtg
     5401 tttgttgccg aattacaaga aatgcaagaa acacaagttg ataatgacgc ttacgatgat
     5461 aacgagatag aaattattcg ttcaaaagaa ttcagcttaa aaccaatgga ttcagaagaa
     5521 gcggtattac aaatgaatct attaggtcat gacttctttg tattcacaga cagagaaact
     5581 gatggaacaa gtatcgttta ccgccgtaaa gacggtaaat atggcttgat tcaaactagt
     5641 gaacaataaa ttaagtttaa agcacttgtg tttttgcaca agtgcttttt tatactccaa
     5701 aagcaaatta tgactatttc atagttcgat aatgtaattt gttgaatgaa acatagtgac
     5761 tatgctaatg ttaatggatg tatatatttg aatgttaagt taataatagt atgtcagtct
     5821 attgtatagt ccgagtcgaa aatcgtaaaa tatttataat ataatttatt aggaagtata
     5881 attgcgtatt gagaatatat ttattagtga taaacttgtt gacaacagaa tgtgaatgaa
     5941 gtatgtcata aatatattta tattgattct acaaatgagt aaataagtat aattttctaa
     6001 ctataaatga taagatatat tgttgtaggc caaacagttt tttagctaaa ggagcgaacg
     6061 aaatgggatt tttatcaaaa attcttgatg gcaataataa agaaattaaa cagttaggta
     6121 aacttgctga taaagtaatc gctttagaag aaaaaacggc aattttaact gatgaagaaa
     6181 ttcgtaataa aacgaaacaa ttccaaacag aattagctga cattgataat gtcaaaaagc
     6241 aaaatgatta tttagataaa attttaccag aagcatatgc acttgttaga gaaggctcta
     6301 aacgtgtatt caatatgaca ccatataaag ttcaaattat gggtggtatt gcaattcata
     6361 aaggtgatat cgctgagatg agaacaggtg aaggtaaaac attaacagcg acaatgccaa
     6421 catacttaaa tgcattagct ggtagaggtg ttcacgttat tacagtcaat gaatacttat
     6481 caagtgttca aagtgaagaa atggctgagt tatataactt cttaggtttg actgtcggat
     6541 taaacttaaa cagtaagacg acagaagaaa aacgtgaagc atacgcacaa gacattactt
     6601 acagtactaa taatgagcta ggttttgatt acttacgaga taacatggtg aattattctg
     6661 aagatagagt aatgcgtcca ttacattttg caatcattga tgaggttgac tcaattttaa
     6721 tcgacgaggc acgtacgcca ttaattattt ctggtgaagc tgaaaagtca acgtcacttt
     6781 atacacaagc aaatgttttt gcgaaaatgt taaaacagga cgaagattat aaatacgatg
     6841 aaaaaacgaa agctgtacat ttaacagaac aaggtgcgga taaagctgaa cgtatgttca
     6901 aagttgaaaa cttatatgat gtacaaaatg ttgatgttat tagtcatatc aacacagctt
     6961 tacgtgcgca cgttacatta caacgtgacg tagactatat ggttgttgat ggcgaagtat
     7021 taattgtcga tcaatttaca ggacgtacaa tgccaggccg tcgtttctcg gaaggtttac
     7081 accaagctat tgaagcgaag gaaggcgttc aaattcaaaa tgaatctaaa actatggcgt
     7141 ctattacatt ccaaaactat ttcagaatgt acaataaact tgcgggtatg acaggtacag
     7201 ctaaaactga agaagaagaa tttagaaata tttataacat gacagtaact caaattccga
     7261 caaataaacc tgtgcaacgt aacgataagt ctgatttaat ttacattagc caaaaaggta
     7321 aatttgatgc agtagtagaa gatgttgttg aaaaacacaa ggcagggcaa ccagtgctat
     7381 taggtactgt tgcagttgag acttctgaat atatttcaaa tttacttaaa aaacgtggta
     7441 tccgtcatga tgtgttaaat gcgaaaaatc atgaacgtga agctgaaatt gttgcaggcg
     7501 ctggacaaaa aggtgccgtt actattgcca ctaacatggc tggtcgtggt acagatatca
     7561 aattaggtga aggcgtagag gaattaggcg gtttagcagt aataggtaca gagcgacatg
     7621 aatctcgtcg tattgatgac cagttacgtg gtcgttctgg acgtcaaggt gataaagggg
     7681 atagtcgctt ctatttatca ttacaagatg aattaatgat tcgttttggt tctgaacgtt
     7741 tacagaaaat gatgagccga ctaggtttag atgactctac accaattgaa tcaaaaatgg
     7801 tatcaagagc tgtagaatca gcacaaaaac gtgtagaagg taataacttc gacgcgcgta
     7861 aacgtatctt agaatacgat gaagtattac gtaaacaacg tgaaattatc tataacgaaa
     7921 gaaatagtat tattgatgaa gaagacagct ctcaagttgt agatgcaatg ctacgttcaa
     7981 cgttacaacg tagtatcaat tactatatta atacagcaga tgacgagcct gaatatcaac
     8041 cattcatcga ctacattaat gacatcttct tacaagaagg tgacattaca gaggatgata
     8101 tcaaaggtaa agatgctgaa gatattttcg aagtcgtttg ggctaagatt gaagcagcat
     8161 atcaaagtca aaaagatatc ttagaagaac aaatgaatga gtttgagcgt atgattttac
     8221 ttcgttctat tgatagccat tggactgatc atatcgacac aatggatcaa ttacgtcaag
     8281 gtattcactt acgttcttat gcacaacaaa atccattacg tgactatcaa aatgaaggtc
     8341 atgaattatt tgatatcatg atgcaaaata ttgaagaaga tacttgtaaa ttcattttaa
     8401 aatctgtagt acaagttgaa gataatattg aacgtgaaaa aacaacagag tttggtgaag
     8461 cgaagcacgt ttcagctgaa gatggtaaag aaaaagtgaa accgaaacca atcgttaaag
     8521 gcgatcaagt tggtcgtaac gatgattgtc catgtggtag tggtaaaaaa ttcaaaaatt
     8581 gccatggaaa ataaatgata taaaataact ccttccaatt aaacacctat agtttgtgtt
     8641 atgggaggag tctttttatt ttacaagcgt taaatacttt aaaaaatgtg aagaagttgt
     8701 taaacgttgt tatgtactta gttttaaaaa atcggtttag gcatatgtcg atgataaatg
     8761 tactgatttt taacaataaa tgcataaact aattgtcagt gtgcttatat ttcttaacat
     8821 tgttatttaa caaaattatg ttaaaattta gcattataaa agatgcaaat caatgacttg
     8881 aattgaaata taaataggag cgaatgctat ggaattatca gaaatcaaac gaaatataga
     8941 taagtataat caagatttaa cacaaattag ggggtctctt tgacttagag aacaaagaaa
     9001 ctaatattca agaatatgaa gaaatgatgg cagaacctaa tttttgggat aaccaaacga
     9061 aagcgcaaga tattatagat aaaaataatg cgttaaaagc aatagttaat ggttataaaa
     9121 cactacaagc agaagtagat gacatggatg ctacttggga tttattacaa gaagaatttg
     9181 atgaagaaat gaaagaagac ttagagcaag aggtcattaa ttttaaggct aaagtggatg
     9241 aatacgaatt gcaattatta ttagatgggc ctcacgatgc caataacgca attctagagt
     9301 tacatcctgg tgcaggtggc acggagtctc aagattgggc taatatgcta tttagaatgt
     9361 atcaacgtta ttgtgagaag aaaggcttta aagttgaaac tgttgattat ctacctgggg
     9421 atgaagcggg gattaaaagt gtaacattgc tcatcaaagg gcataatgct tatggttatt
     9481 taaaagctga aaaaggtgta caccgactag tacgaatttc tccatttgat tcatcaggac
     9541 gtcgtcatac atcatttgca tcatgcgacg ttattccaga ttttaataat gatgaaatag
     9601 agattgaaat caatccggat gatattacag ttgatacatt cagagcttct ggtgcaggtg
     9661 gtcagcatat taacaaaact gaatcggcaa tacgaattac ccaccacccc tcaggtatag
     9721 ttgttaataa ccaaaatgaa cgttctcaaa ttaaaaaccg tgaagcagct atgaaaatgt
     9781 taaagtctaa attatatcaa ttaaaattgg aagagcaggc acgtgaaatg gctgaaattc
     9841 gtggcgaaca aaaagaaatc ggctggggaa gccaaattag atcatatgtt ttccatccat
     9901 actcaatggt gaaagatcat cgtacgaacg aagaaacagg taaggttgat gcagtgatgg
     9961 atggagacat tggaccattt atcgaatcat atttaagaca gacaatgtcg cacgattaat
    10021 atatatttta aaaccgaggc tctaaaaggg cgtcggtttt tggttttttt aaaggtagct
    10081 aaataaattg taaattagat tttggaatat gatttgttta tgaatattta agtacaattc
    10141 ggtagataga gttagaatat attttttaaa agttgtgttt gttaaaacaa catatgcagt
    10201 gtgcatttag taatattacc tatggcgatt ttcaaggtat tgaattaatt attgaaaacg
    10261 ttctcaatta catggtatga atacatttta cactatgata aaaggttgta ttctttttat
    10321 attgttaacc atttgattac atcgttataa caatagcttt tgacaaaatg tattgtgcta
    10381 tagtatttgc atacttaaaa tactaacagc aaaggaatga cagcaagatg aaaaaatctc
    10441 ttacagtgac ggtttcgtca gtgttagctt ttttagcttt aaataatgca gcacatgcac
    10501 aacaacatgg cacacaagta aaaacacctg ttcaacataa ttatgtatca aatgttcaag
    10561 cacaaacgca atcaccgaca acttatacag tagttgctgg cgattcatta tataagattg
    10621 ctttagagca tcacttaacg ttgaatcaat tatattcata caatcctggt gtaacacctt
    10681 taatttttcc tggtgacgtg atttcacttg tgcctcaaaa taaagtgaaa caaactaaag
    10741 cggttaaatc accagtaaga aaagcaagcc aagctaaaaa ggtagtaaaa caacctgtac
    10801 aacaagcatc taaaaaagta gtagttaagc aagcacctaa gcaagcagta actaagacag
    10861 ttaatgtagc atacaaacct gctcaagtac aaaaatcagt accaactgta cctgttgcac
    10921 ataactacaa taaatcagtt gctaacagag gaaacttata tgcttatgga aactgcacat
    10981 attatgcttt cgatcgtcgt gcacaattag gtagaagtat aggaagttta tggggcaatg
    11041 caaataactg gaattacgca gcaaaagttg caggatttaa agtagataaa acaccagaag
    11101 ttggcgctat tttccaaaca gctgctggtc catatggaca tgttggtgtt gttgaatctg
    11161 taaaccctaa tggaacaatt actgtttctg aaatgaacta tgctggattt aatgttaaat
    11221 cttcaagaac aattttaaat ccaggaaaat ataattacat ccactaagta atatatcaag
    11281 acaagactat cctcttagcc tgtttaagta acaggttgag aggatttttt ggtatcattt
    11341 aatcagagtt atataaagaa gatatttaaa tgatatttaa ttgattgata tgtaaaaaga
    11401 aagtataatt tatgattaat taatggagga ggtaattgaa atgggtgtac atcaatattt
    11461 taaaagatta tcagatatgg aaagacttat aagattacct ggaaaattta aatattttga
    11521 acacaatgtt gcagcacact cctttaaagt aactaaaatt gcacaatatc tagcaacagt
    11581 tgaagaatat catggacgaa agattaattg gaaaagctta tatgaaaaag cattaaatca
    11641 tgatttcgcc gaagtgttta ctggtgatat aaaaacacct gttaaatatg cgagtagtga
    11701 attaaaaaaa ttattttcgc aagttgaaga agaaatggta gagaccttta ttgaagaaga
    11761 aattccatta caatatagag atgtttataa gcaacgactg caagaaggta aagatgattc
    11821 attagaaggc caaatacttt cagttgctga taaaattgat ttgctttatg aaacatttgg
    11881 agaaatacaa aaacgtaatc ccgaagaatt atttttcgaa atttatgaaa tgagtctaga
    11941 aacaattatt caatttgacc atttagcatc tgtacaagat tttattaata atatcattcc
    12001 agaaatgttg actgaaaact ttatacctag aacagaatta agagaaacaa ccatgaacat
    12061 tttaaataaa agaaaagagg aaaatgaatg atatggtatt ttagcgcagc attctttcca
    12121 tgtgtcttgg tagtattatt tagtgtaata acaagaagta aatgggtcgg tactattctg
    12181 acattaattt taattggtgc ctcaatctat aaagagtatt tccataacga gtggattatt
    12241 tttattgatg tagtgtcatt attagctggt tatttaatta tagatcaact cgaatttcat
    12301 aaacatcaag atgaagatcg ctaagattaa ctttaaaata atgtttcaaa caaatttgtt
    12361 gaaacaaaat gatgattaat ataatgtgta tttacatact aaaaataaca agataaacga
    12421 tttgatttaa ggcaagcata gttagcacaa ctatgtttgt ttttctttgt tcgacatttt
    12481 tacgaacaaa cgtttgcttt ttgtgtgact actttgctaa aatatgtaat gagaaagcaa
    12541 agagttgagc ggaaatataa ataatacgtt gaaagaggag gcatatgtga caatggttga
    12601 acattatcct tttaaaatac attctgattt tgagcctcaa ggtgaccaac cgcaagcaat
    12661 taaagaaatc gtggaaggta ttaaagcggg gaaaagacat caaactttat taggtgctac
    12721 tggcacaggg aaaacattta cgatgagtaa tgttattaaa gaagttggga aaccaacgtt
    12781 aattatcgcg cataacaaaa cattagcagg acaattatat agtgagttta aagaattttt
    12841 tcctgaaaac agggtggaat actttgtaag ttactatgat tattatcaac cagaggcata
    12901 cgtaccgtct actgacactt ttattgaaaa agatgcctca atcaatgatg aaattgatca
    12961 actacgacat tctgctacaa gtgcattatt tgaacgcgat gatgtaatta ttattgctag
    13021 tgtaagttgt atatatggtt taggtaatcc tgaagaatat aaagatttag tagtaagtgt
    13081 tcgagttggt atggaaatgg atagaagtga attacttaga aaacttgtag atgtgcaata
    13141 tacacgaaat gacatcgatt tccaacgagg aacgtttcga gtgcgtggtg atgtagtgga
    13201 aatattccca gcctctaaag aagaactttg tataagggtt gagtttttcg gcgatgagat
    13261 tgaccgtatc cgagaagtta actacctaac aggtgaagtg ttgaaagaaa gagaacattt
    13321 tgcgatattc ccagcttctc acttcgtaac acgtgaagaa aagttgaaag ttgcgattga
    13381 acgtattgaa aaagaattgg aagaacgatt gaaagaatta cgagatgaga ataaattact
    13441 agaagcgcaa aggttagaac agcgtaccaa ctatgattta gaaatgatgc gagagatggg
    13501 attctgttca ggaattgaaa actattccgt acatttaact ttgcgaccac tgggttcgac
    13561 accatatact ttattggatt actttggcga tgattggtta gtaatgattg atgaatcaca
    13621 tgtgacatta ccgcaagttc gaggcatgta taacggagac agagcgcgta aacaagtttt
    13681 ggtggatcat gggtttagat taccgagtgc attagataac cgtccactta aatttgaaga
    13741 atttgaagaa aagacaaaac aacttgtgta tgtatctgca acgcctggac catacgaaat
    13801 tgaacatacg gataagatgg ttgaacaaat tattcgtcct actggtttac tggatcctaa
    13861 gattgaggtt agacctactg aaaatcaaat tgacgattta ttaagtgaaa ttcaaacaag
    13921 agttgagcgt aatgaacgcg tacttgttac aacgctcact aaaaagatga gtgaagattt
    13981 aaccacatac atgaaagaag cgggtattaa agttaattat ctgcattcag aaatcaagac
    14041 attagaacga attgaaataa ttagagactt acgaatgggt acatatgatg ttatcgtagg
    14101 tattaattta ttaagagagg gtattgatat accagaagtt tctctagttg tcatattaga
    14161 tgcagataaa gaagggtttt tacgttctaa ccgctcatta attcaaacaa taggtagagc
    14221 tgcgcgtaac gataaaggtg aagtcattat gtatgccgat aaaatgactg attcgatgaa
    14281 gtatgcaatt gatgagacac aacgtcgtcg agaaatacag atgaaacata atgaaaaaca
    14341 tggtattaca cctaaaacaa ttaataaaaa aatacatgat ttaattagtg ctactgttga
    14401 aaatgacgaa aataatgaca aagcacaaac tgtgatacct aagaagatga cgaaaaaaga
    14461 acgtcaaaag acaatcgaca atatagaaaa agaaatgaaa caagcagcga aagatttaga
    14521 tttcgagaaa gctacagaat taagagatat gttatttgaa ttaaaagcag aagggtgaca
    14581 agtaaatgaa agaaccatcc atagtagtaa aaggtgctcg tgcgcataac ttgaaagata
    14641 ttgatatcga actacctaaa aataaattaa ttgttatgac aggtttatct gggtcaggta
    14701 aatcgtcatt agcattcgat actatatatg ctgaaggaca acgacgttat gttgaatcat
    14761 taagtgccta tgcgcgtcaa tttttaggcc aaatggacaa accagatgtt gatacaattg
    14821 aaggattatc gccagcaatt tcaatagatc aaaaaacaac aagtaaaaat ccaagatcaa
    14881 ctgtagcaac agtaacagaa atatatgatt atatacgttt gttatatgca cgtgttggta
    14941 aaccttactg tccaaatcac aatatagaaa ttgaatcgca aacagtacaa caaatggttg
    15001 accgcattat ggaattagag gcacgtacaa agattcaatt attagcacct gtcatcgctc
    15061 atcgtaaagg tagtcatgaa aagctaatcg aagatattgg taaaaaaggt tatgtacgtt
    15121 taagaatcga tggcgaaatt gttgatgtaa atgatgtacc tactttagat aagaacaaga
    15181 atcatacaat agaagttgtt gtagaccgat tagttgttaa agatggaatt gaaacacgac
    15241 tagctgactc tatagaaact gccttagagc tttcagaagg acaattaaca gtcgatgtca
    15301 ttgacgggga agaccttaag ttttcagaaa gccatgcttg tcctatatgt ggattttcaa
    15361 tcggagagtt agaaccaaga atgtttagct ttaacagtcc ttttggtgct tgtccgacat
    15421 gtgatggctt aggccaaaag ttaacagtcg atgtagactt ggttgttccc gacaaagata
    15481 agacgctaaa cgaaggtgca atagaacctt ggataccgac gagttctgat ttttatccaa
    15541 cattgttaaa acgtgtttgt gaagtttata aaatcaatat ggataaacct tttaaaaagt
    15601 taacagaacg tcaacgtgat attttattgt atggttctgg tgacaaagaa attgaattta
    15661 catttacaca acgtcaaggt ggtactagaa aacgaacaat ggttttcgag ggtgtagttc
    15721 ctaatataag tagacgattc catgaatctc cttcagaata tacacgtgaa atgatgagta
    15781 aatatatgac tgaactacct tgcgaaactt gtcatggaaa gcgattgagt cgtgaagcgt
    15841 tatctgttta tgtaggtggt ttaaatattg gtgaagtagt cgaatattca atcagtcaag
    15901 cgctgaacta ttataaaaac attgatttgt cagaacatga tcaagcgatt gcaaatcaaa
    15961 tattgaaaga aattatttcc cgactcactt ttttaaataa tgtgggactt gaatatttaa
    16021 cgttaaacag agcttcaggt acactttcag gtggtgaagc acaacgtatt cgattagcaa
    16081 cgcaaattgg gtcgcgtttg actggtgtct tatatgtatt agatgagcca tcaattggac
    16141 tgcatcaaag agataatgat cgattaatta atacacttaa agaaatgaga gatttaggaa
    16201 atactttaat tgtagttgaa cacgatgatg atacaatgcg tgcggctgat tacttagtgg
    16261 atataggtcc tggtgctggt gaacatggag ggcagattgt gtctagtggt actcctcaaa
    16321 aggtaatgaa agataaaaaa tcattaacag gacaatactt gagtggtaag aaacgtattg
    16381 aagtacctga atatcgcaga ccggcttcag atcgtaaaat ttctatacgt ggagctagaa
    16441 gcaacaatct taaaggggtt gatgtggaca taccactatc aatcatgacg gttgttacag
    16501 gtgtatcagg ttctggtaaa agctcattag taaatgaagt attatacaaa tcattagctc
    16561 aaaaaattaa taaatctaaa gtaaagccag gattgtacga taagattgaa ggtattgatc
    16621 aacttgataa aattattgat attgatcaat caccaatagg tagaacgcca cgctctaatc
    16681 cagcaacata tactggtgtg tttgatgata tacgtgatgt gtttgcgcaa acaaatgaag
    16741 ctaaaattcg aggatatcaa aaagggcgtt ttagttttaa tgtaaaaggt ggacgctgtg
    16801 aagcttgtaa aggtgacggt attattaaaa ttgaaatgca ttttttacct gatgtttatg
    16861 ttccttgtga agtgtgtgat ggtaaacgat ataatcgtga gacactagag gttacttaca
    16921 aaggtaaaaa tattgctgac attttagaaa tgactgttga agaagcaaca caattttttg
    16981 aaaatattcc taagattaag cgcaagttac aaacactagt tgatgttggt cttggatacg
    17041 tcacattagg tcaacaagct acaacgttat caggtggtga ggctcaacgt gtgaaacttg
    17101 catctgaact tcataaacgt tcaactggta aatctattta tatcctagat gaaccgacaa
    17161 cagggttaca tgttgacgat attagtagat tattaaaagt attaaaccga ttagttgaaa
    17221 atggtgatac tgttgtaatt attgaacata acctagatgt tatcaaaaca gcagactata
    17281 ttatagactt aggtcctgaa ggtggtagtg gcggtggtac tattgttgcg actggcacac
    17341 ccgaagatat tgctcagaca aagtcatcat atacaggaaa gtatttaaaa gaagtacttg
    17401 aacgagataa acaaaatact gaagataaat aagattaaaa gaagtgaagg atgttataaa
    17461 tttatccttc gcttcttttt attaatttag taatgaatag tagaaagaaa agatgcgtaa
    17521 aaagaattat gttaagatag ggtcaatcta gagtagttaa acataaatcg aactgggagt
    17581 gggacagaaa tgataaagaa tcactaatga tttattatgt agtggttctt tgtcattagc
    17641 cacagctatt gtgtacttaa aaataggaat gcatgagtgc aactcatgca taagaaatac
    17701 taatttctaa agaaaaagta tttctttatg ttggggcccc gccaacttgc attgtttgta
    17761 gaatttcttt tcgaaattct ttatgttggg gccccgccaa ctaattccaa tatatcattg
    17821 tagagcttag gtcattgatt tttggctcgg acttttatgg cgatatgaac catgtaaatt
    17881 aagcaagcaa taaattaatg attgatattg acttgtaaaa taataacaat aatgaacaat
    17941 taatatttat tttagctttt caatgtagat tggtgttata tttttgatat gataagaaga
    18001 gatgtaagag tagggataaa tacaattgag gtgaacccat gttaacgaca gaaaaactag
    18061 ttgaaacatt aaagttagat ttaatcgctg gtgaagaagg actatcgaag ccaattaaaa
    18121 atgctgatat atcaagaccg ggcttagaga tggcaggtta tttttcacat tatgcgtcag
    18181 atagaataca actattagga acaacggaac tatcgtttta caatttatta ccagataagg
    18241 atcgcgcagg tcgtatgcgt aaactatgca gaccagaaac gcctgcaatt attgtgacac
    18301 gtggattgca gccaccagaa gaattagttg aagctgcaaa agaattaaat accccactta
    18361 tagttgctaa agatgcgact acaagtttaa tgagtcgctt aacaacgttt ttagagcatg
    18421 cacttgcaaa gacgacatct ttacatggtg ttttagtaga tgtttacggt gttggtgtac
    18481 taattaccgg tgattcagga ataggtaaaa gtgagactgc gttggaatta gttaaacgtg
    18541 ggcatagatt agtagcagat gataatgtag aaatacgtca aattaataaa gatgaactaa
    18601 tagggaaacc accaaagtta atagaacatc tattagaaat acgtggacta ggtattatca
    18661 atgttatgac tttatttggc gcgggttcaa tattaactga aaaacgaatt agattaaata
    18721 ttaatttgga aaactggaac aagcaaaagt tatatgaccg cgtaggtctt aatgaagaga
    18781 cgctaagtat tttagatact gaaatcacta aaaaaacaat acctgtaaga cctggtagaa
    18841 atgttgcggt aattattgag gtcgctgcaa tgaactatcg attaaatatc atgggcatta
    18901 acacggccga agaatttagt gaaagattaa atgaagaaat tatcaagaac agtcataaga
    18961 gtgaggagta ggttgaatgg gtattgtatt taactatata gatcctgtgg catttaactt
    19021 aggaccactg agtgtacgat ggtatggaat tatcattgct gtcggaatat tacttggtta
    19081 ctttgttgca caacgtgcac tagttaaagc aggattacat aaagatactt tagtagatat
    19141 tattttttat agtgcactat ttggatttat cgcggcacga atctattttg tgattttcca
    19201 atggccatat tacgcggaaa atccaagtga aattattaaa atatggcatg gtggaatagc
    19261 aatacatggt ggtttaatag gtggctttat tgctggtgtt attgtatgta aagtgaaaaa
    19321 tttaaaccca tttcaaattg gtgatatcgt tgcgccaagt ataattttag cgcaaggaat
    19381 tggacgctgg ggtaacttta tgaatcacga ggcacatggt ggatcggtgt cacgcgcttt
    19441 tttagaacaa ttacatttgc ctaattttat aatagaaaat atgtatatta acggccaata
    19501 ttatcatcca acattcttat atgaatccat ttgggatgtc gctggattta ttatcttagt
    19561 taatattcgt aaacatttaa aattaggaga aacattcttt ttatatttaa cttggtattc
    19621 aattggtcga ttctttatag aaggattacg tacagatagc ttaatgctca caagtaatat
    19681 tagagttgca caattagtat caattctttt aattttaata agtataagtt taattgtata
    19741 tagaaggatt aagtataatc caccgttgta tagcaaagtt ggggcgcttc catggccaac
    19801 aaaaaaagtg aagtagtgat agtttgagga aatttttatc aaaaacacat catcatacaa
    19861 accctttatg gcgtgtatac cgtcttgtta aattttcgaa agtttttaag aatgtaatta
    19921 tcattgaatt ttcgaaattt attccaagta tggtactgaa aagacatata tataaacaac
    19981 ttttaaatat taatatcggt aatcaatcgt cgatagctta taaagtaatg ttagatattt
    20041 tttacccaga actgattacg attggtagta acagtgttat tggttacaat gtaacaattt
    20101 tgacgcatga agcattagtt gatgaatttc gttatggacc agtgacgata ggatctaaca
    20161 ctttgattgg tgcaaatgct accattttac ccggtataac gattggtgac aatgtaaaag
    20221 ttgcagctgg tacggttgtt tcaaaagata taccggataa tggatttgca tatggcaacc
    20281 ctatgtatat aaaaatgatt aggaggtgac aattttatgg cgcaaaagaa taataatgta
    20341 attccaatga cttttgatga tgcattttat cgtaaaatgg ctaaacagaa gtttaaacaa
    20401 agagaatata aacgagctgc tgaatacttt gaaaaagtgt tagaattgtc acctgatgat
    20461 atggaaattc aaattgatta tgcacaatgt ctagtgcaac ttggtattgc taaaaaagca
    20521 gaacatttat tttatgacaa tattatttat aataggcatc tagaagatag cttttatgaa
    20581 ttgagtcagc tcaacattga agttaacgaa ccaaacaagg cattcttgtt tggtattaat
    20641 tatgttattg ttagcgacga ccaagattat agagatgaat tagatcaaat gtttgatgtg
    20701 aaatatcaaa gtgaagaaca aattgaactt gaagctcaat tgtttgtagt tcaaatacta
    20761 ttccaatatc ttttttctca aggtcgatta aaagatgcaa agaattatgt cttacatcaa
    20821 ccacaagaag ttcaagatca tcgtgtagta cgtaatttat tggcaatgtg ttatttatat
    20881 ctcggtgaat atgatacggc taaagcattg tacgaagcac tattacaaga ggatagtaca
    20941 gatatatatg cattatgcca ttatactttg ctactttata acactaagga aaatgaacaa
    21001 tatcaaaaat atttaaaaat attaaacaaa gttgtaccta tgaatgacga tgaaagtttt
    21061 aaattaggta ttgtattaag ttatttaaag cagtatcgtg catcacaaca attgttgtac
    21121 cctttatata aaaaagggaa atttttatca attcaaatgt acaatgcttt agcatataat
    21181 tattattatt taggtgaaga agacgaaagt cattactact gggataaatt gaagcaaatt
    21241 tctaaagtgg aaattggaca tgcgccttgg gtaattgaaa atagcaaaga agtttttgac
    21301 caacatattt tgccattact tcaaagtgat gacagtcatt atcgtttata tggtattttt
    21361 ttattggatc aattaaatgg taaagaaatt gtgatgacgg aaagtatttg gcaggttttg
    21421 gaaaatctaa ataattatga gaaattgtat ttaacgtatt tagttcaagg tttaacgctc
    21481 aataaattag acttcattca tcgcggctta ttaacgcttt accataatga attatttgta
    21541 agtgaaaatg atgtaatggt tgcatggatt aatcaaggtg aactcataat tgctgaaaaa
    21601 gtagatttaa ctgatgttga gccatatatc ggtgcgttta tttatttgta ttttaaaaat
    21661 caacctcgaa acgttacaaa gaagcaaatt acaacatggt taggcataac acaatataaa
    21721 ctgaacaaaa tgattgaatt tctcttgagc atatagattt atgaaaagtt agatttatta
    21781 tataatgcgc ataatgatta ataatgagga ggcgttaata aaatgactga aatagatttt
    21841 gatatagcaa ttatcggtgc aggtccagct ggtatgactg ctgcagtata cgcatcacgt
    21901 gctaatttaa aaacagttat gattgaaaga ggtattccag gcggtcaaat ggctaataca
    21961 gaagaagtag agaacttccc tggtttcgaa atgattacag gtccagattt atctacaaaa
    22021 atgtttgaac acgctaaaaa gtttggtgca gtttatcaat atggagatat taaatctgta
    22081 gaagataaag gcgaatataa agtgattaac tttggtaata aagaattaac agcgaaagcg
    22141 gttattattg ctacaggtgc agaatacaag aaaattggtg ttccgggtga acaagaactt
    22201 ggtggacgcg gtgtaagtta ttgtgcagta tgtgatggtg cattctttaa aaataaacgc
    22261 ctattcgtta tcggtggtgg tgattcagca gtagaagagg gaacattctt aactaaattt
    22321 gctgacaaag taacaatcgt tcaccgtcgt gatgagttac gtgcacagcg tattttacaa
    22381 gatagagcat tcaaaaatga taaaatcgac tttatttgga gtcatacttt gaaatcaatt
    22441 aatgaaaaag acggcaaagt gggttctgtg acattaacgt ctacaaaaga tggttcagaa
    22501 gaaacacacg aggctgatgg tgtattcatc tatattggta tgaaaccatt aacagcgcca
    22561 tttaaagact taggtattac aaatgatgtt ggttatattg taacaaaaga tgatatgaca
    22621 acatcagtac caggtatttt tgcagcagga gatgttcgcg acaaaggttt acgccaaatt
    22681 gtcactgcta ctggcgatgg tagtattgca gcgcaaagtg cagcggaata tattgaacat
    22741 ttaaacgatc aagcttaatt cgaagtcgaa ttaagatgtt gagctgtaaa ttatttggat
    22801 atttatttta atagtgtcat cacagcgtta aaataatgtc ttacttttaa attaaagcaa
    22861 attatataga aaactagaac ttagtacgta tcatttgtgc gtttcaatga gttctagttt
    22921 ttttatatgt tatattaaac ttataacttt a
//
LOCUS       DALAAR010000042        22752 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000042 DALAAR010000000
VERSION     DALAAR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.044"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            94..1032
                     /gene="lukS-PV"
                     /locus_tag="O2458_001683"
     CDS             94..1032
                     /gene="lukS-PV"
                     /locus_tag="O2458_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCZ3887548.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            1028..2011
                     /gene="lukF-PV"
                     /locus_tag="O2458_001684"
     CDS             1028..2011
                     /gene="lukF-PV"
                     /locus_tag="O2458_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCZ3887549.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2260..2445
                     /locus_tag="O2458_001685"
     CDS             2260..2445
                     /locus_tag="O2458_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887550.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2536..3453
                     /locus_tag="O2458_001686"
     CDS             2536..3453
                     /locus_tag="O2458_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ3887551.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3510..4448
                     /locus_tag="O2458_001687"
     CDS             3510..4448
                     /locus_tag="O2458_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ3887552.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            4441..4647
                     /locus_tag="O2458_001688"
     CDS             4441..4647
                     /locus_tag="O2458_001688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887553.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            4662..5576
                     /locus_tag="O2458_001689"
     CDS             4662..5576
                     /locus_tag="O2458_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ3887554.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            5775..5894
                     /locus_tag="O2458_001690"
     CDS             5775..5894
                     /locus_tag="O2458_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887555.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      5977..6111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6173..6718
                     /locus_tag="O2458_001691"
     CDS             6173..6718
                     /locus_tag="O2458_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ3887556.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(6824..7072)
                     /locus_tag="O2458_001692"
     CDS             complement(6824..7072)
                     /locus_tag="O2458_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCZ3887557.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            7180..8133
                     /locus_tag="O2458_001693"
     CDS             7180..8133
                     /locus_tag="O2458_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3887558.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            8123..9502
                     /locus_tag="O2458_001694"
     CDS             8123..9502
                     /locus_tag="O2458_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCZ3887559.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            9655..11115
                     /gene="ebpS"
                     /locus_tag="O2458_001695"
     CDS             9655..11115
                     /gene="ebpS"
                     /locus_tag="O2458_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCZ3887560.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            11240..11356
                     /locus_tag="O2458_001696"
     CDS             11240..11356
                     /locus_tag="O2458_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887561.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            11517..12503
                     /locus_tag="O2458_001697"
     CDS             11517..12503
                     /locus_tag="O2458_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCZ3887562.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(12618..13586)
                     /locus_tag="O2458_001698"
     CDS             complement(12618..13586)
                     /locus_tag="O2458_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCZ3887563.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            13663..14322
                     /locus_tag="O2458_001699"
     CDS             13663..14322
                     /locus_tag="O2458_001699"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCZ3887564.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            14353..14469
                     /locus_tag="O2458_001700"
     CDS             14353..14469
                     /locus_tag="O2458_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887565.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            14586..14777
                     /locus_tag="O2458_001701"
     CDS             14586..14777
                     /locus_tag="O2458_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887566.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            15034..16209
                     /gene="rpsA"
                     /locus_tag="O2458_001702"
     CDS             15034..16209
                     /gene="rpsA"
                     /locus_tag="O2458_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCZ3887567.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            16431..17741
                     /gene="der"
                     /locus_tag="O2458_001703"
     CDS             16431..17741
                     /gene="der"
                     /locus_tag="O2458_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCZ3887568.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            17758..18756
                     /locus_tag="O2458_001704"
     CDS             17758..18756
                     /locus_tag="O2458_001704"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCZ3887569.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            18927..19199
                     /locus_tag="O2458_001705"
     CDS             18927..19199
                     /locus_tag="O2458_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCZ3887570.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            19630..20202
                     /locus_tag="O2458_001706"
     CDS             19630..20202
                     /locus_tag="O2458_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCZ3887571.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            20205..20930
                     /locus_tag="O2458_001707"
     CDS             20205..20930
                     /locus_tag="O2458_001707"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCZ3887572.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            20932..21891
                     /locus_tag="O2458_001708"
     CDS             20932..21891
                     /locus_tag="O2458_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ3887573.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            21983..22432
                     /gene="ndk"
                     /locus_tag="O2458_001709"
     CDS             21983..22432
                     /gene="ndk"
                     /locus_tag="O2458_001709"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCZ3887574.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         8664 a         3171 c         4057 g         6860 t
ORIGIN      
        1 gtaaatcttt taataaatat ttaattttat taaaagttaa aaaggtttaa tataaaaatg
       61 taataaaatt tataaagaaa ggaaatgatt tttatggtca aaaaaagact attagctgca
      121 acattgtcgt taggaataat cactcctatt gctacttcgt ttcatgaatc taaagctgat
      181 aacaatattg agaatattgg tgatggcgct gaggtagtca aaagaacaga agatacaagt
      241 agcgataagt ggggggtcac acaaaatatt cagtttgatt ttgttaaaga taaaaagtat
      301 aacaaagacg ctttgatttt aaaaatgcaa ggttttatca attcaaagac tacttattac
      361 aattacaaaa acacagatca tataaaagca atgaggtggc ctttccaata caatattggt
      421 ctcaaaacaa atgaccccaa tgtagattta ataaattatc tacctaaaaa taaaatagat
      481 tcagtaaatg ttagtcaaac attaggttat aacataggtg gtaattttaa tagtggtcca
      541 tcaacaggag gtaatggttc atttaattat tcaaaaacaa ttagttataa tcaacaaaac
      601 tatatcagtg aagtagaacg tcaaaattca aaaagtgttc aatggggaat aaaagctaat
      661 tcatttatca catcattagg taaaatgtct ggacatgatc caaatttatt tgttggatat
      721 aaaccatata gtcaaaatcc gagagactat tttgttccag acaatgaatt acccccatta
      781 gtacacagtg gtttcaatcc ttcatttatt gcaactgttt ctcatgaaaa aggctcagga
      841 gatacaagtg aatttgaaat aacgtatggc agaaatatgg atgttactca tgctactaga
      901 agaacaacac actatggcaa tagttattta gaaggatcta gaatacacaa cgcatttgta
      961 aacagaaatt acacagttaa atatgaagtg aactggaaaa ctcatgaaat taaagtgaaa
     1021 ggacataatt gatatgaaaa aaatagtcaa atcatcagtt gttacatcaa ttgcattgct
     1081 tttgctatcc aatacagttg atgcagctca acatatcaca cctgtaagtg agaaaaaggt
     1141 tgatgataaa attactttgt acaaaacaac tgcaacatca gattccgata agttaaaaat
     1201 ttctcagatt ttaactttta attttattaa agataaaagt tatgataaag atacattaat
     1261 actcaaagct gctggaaaca tttattctgg ctatacaaag ccaaatccaa aagacactat
     1321 tagttctcaa ttttattggg gttctaagta caacatttca attaattcag attctaatga
     1381 ctcagtaaac gttgtagatt atgcacctaa aaatcaaaat gaagaatttc aagtacaaca
     1441 aacggtaggt tattcttatg gtggagatat taatatctct aacggcttat caggtggagg
     1501 taatggttca aaatcttttt cagagacaat taactataaa caagaaagct atagaactag
     1561 cttagataaa agaactaatt tcaaaaaaat tggttgggat gttgaagcac ataaaattat
     1621 gaataatggt tggggaccat atggcagaga tagttatcat tcaacttatg gtaatgaaat
     1681 gtttttaggc tcaagacaaa gcaacttaaa tgctggacaa aacttcttgg aatatcacaa
     1741 aatgccagtg ttatccagag gtaacttcaa tccagaattt attggtgtcc tatctcgaaa
     1801 acaaaacgct gcaaaaaaat cgaaaattac tgttacttat caaagagaaa tggatagata
     1861 tacaaacttt tggaatcaac ttcactggat aggtaataat tataaagatg aaaatagagc
     1921 aactcataca tcaatttatg aagttgattg ggaaaatcat acagttaaat taatagatac
     1981 tcaatctaag gaaaaaaatc ctatgagcta aacagataga taatcaaaaa atcttaaata
     2041 tgttaaaatt tacaaacact ttctttctat attagggtaa ccacgtctta attgacgtgg
     2101 ttattttttc agggcaaaaa aagggcggat tatttaaata aggacaaaca cttgtggaaa
     2161 atttaaaagg ttaaaaataa taaagaactt ggtataacaa gggttttata catttgcgta
     2221 caacgacgaa atgtcaattt accatcacat tatgacgata tgtttatttt aaacacacaa
     2281 gctcatgcgc gtcttgatca aatggcacaa cagtttgaag ttgtttgtaa tggcttgaac
     2341 gaaaatgaag gacaagcaat tcaaacgatg gatcaatctg cctctctaat acggtcaaac
     2401 ttaattcaag ttaaagaaca attagaaaaa ctagctgtat actaattaat ttattaaatg
     2461 ctacttgttt tcattgagaa taagtagctt tttcaaacat aaaagtttta caaacacata
     2521 aatgggtgat gagctatgtt taaaagaact aaactaatct taatagcaac attactgcta
     2581 tcaggatgtt caactaccaa taacgaatct aataaagaaa caaaatcagt gccagaagaa
     2641 atggaagctt caaaatatgt aggtcaaggc ttccaaccac ctgcagaaaa agatgtggtt
     2701 gaatttgcga aaaagcataa agataaaatt gccaaacgag gcgaacaatt ttttatggat
     2761 aatttcggtc taaaagttaa agctaccaat gttgtaggta gtggaaaagg cgtagaagta
     2821 ttcgtgcatt gtgatgacca cgatatcgta tttaatgcga gtattccatt tgataaatca
     2881 ataattgaga gtgatagctc attaagaagt gaggacaaag gcgatgatat gagtacttta
     2941 gttggtacag tgttgagtgg ctttgaatat cgaacacaaa aagaaaagta tgacaattta
     3001 tataaatttt tcaaagataa tgaagagaaa taccaatata caggctttac aaaagaagca
     3061 ataaacaaga cgcaaaatgt cggatataaa aatgaatatt tttatatcac ttactcgtca
     3121 agaagcttaa aagaatatcg taagtattac gaaccattga ttcataaaaa tgataaagaa
     3181 tttaaagaag gaatggaaca agccagaaaa gaagtgaatt acgctgctaa tacagataca
     3241 gtaacaacat tgtttagtac aaaggaaaat tttactaaag acaatacagt tgatgatgta
     3301 attgaactga gtgataaact atataatttt aaaaataagc cagaaaaatc tacaattaca
     3361 attcaaatag gaaagcctac tattaatact aaaaaagcct tttatgatga taatgatcca
     3421 atagaatatg gagtgtatcg taaagatgaa taaatttttt taaaatgctt tttaatattc
     3481 tatataaaca cataaatggg tgatgagcta tgtttaaaaa ggcgaaatta atcttaatag
     3541 caacgctact gttatcagga tgttcagcga tgaataatga atcaaaaaaa gacacaaata
     3601 cagaaacaaa tacagaaaca aaatcagtac cagaagaaat ggaagcttca aaatatgtag
     3661 gacaaggctt tcaaccacct gcagaaaaag atgcgattga atttgtgaag aagcatcgta
     3721 aagaatttga aaaagtaggt gaacaattct ttaaagataa ctttggacta aaagttaaag
     3781 ctacaaatgt tgtaggtaaa gatgatggtg tagaagttta tgtgcattgc gaagatcatg
     3841 gcattgtatt taatgcaagt ctacctttgt acaaagatgc catccatcaa aaaggatcaa
     3901 tgcgtagtaa tgacaacggt gatgatatga gtatgatggt gggtacagtg ctgagtggct
     3961 ttgaatatcg agcgcaaaaa gaaaagtatg ataatttata taaattttta aaagaaaatg
     4021 aaaagcaata tcaatataca ggttttacta aagaagcaat taacaagacg caaaacgtcg
     4081 ggtatcaaaa tgaatatttt tatattacat atttatcaag aaatttaaaa gaatatagaa
     4141 aatattacga accattgata cataaaaatg ataaagagtt taaagaaggt atgcaacgag
     4201 ctagaaaaga gctaaactat actgctaata caaatactgt agcaacgttg tttagtacga
     4261 atgatgaaag gaatagaaaa gaaaagataa ataatgtaat agatttatcc gagaaaattg
     4321 aaagaacaaa agatatgcca atcaagaata ctataactac tcaattagga aataaactta
     4381 ttggcacaaa aaaagctcgt tttgatgata agaaagtagt gtcgtttgga gcatttgaag
     4441 atgaataaaa taaatgatag agatttaaca gaattgagta gctatagggt ttatcaagac
     4501 atcaataaag ataatgactt tacagttaac gaaaaacgat ttaagcaggc agatgtattt
     4561 gaagatttat atagagagaa actaaaagac acaaataaat taagagagta taattattta
     4621 caaaatgaaa cttttaaaag cgcataaata ggtgatgaga tatgcttaaa aaagcaaaat
     4681 ttatcttaat ggcaacgata ctactatcag gatgttcaac taccaataac gaatccaaca
     4741 aagaaacaaa atctgtacca gaagaaatgg atgcttcaaa atatgtagga caaggattcc
     4801 aaccacctgc agaaaaagat gcgattgaat ttgcaaagaa gcataaagat aaaattgcta
     4861 agcgaggcga acaatttttt atggataact tcggtctaaa agttaaagct acaaatgtta
     4921 taggtagtgg cgatggtgta gaagtattcg tgcattgtga tgaccacgac atcgtattta
     4981 atgcgagtat tccatttgat aaatcaatta ttgacagtga tagctcatta agaagtaagg
     5041 ataaaggtga tgatatgagt actttagttg gtgcagtact cagtgggttt gaatatcgag
     5101 cacaaaaaga aaaatatgat aaattatata aatttttcaa agataatgaa gagaaatatc
     5161 aatatacagg atttacaaaa gaagcaatta ataagacgca aaatagtggt tatgaaaatg
     5221 aatattttta tatttcggcc ataccttata atttagctga gtatagagac tattttgaac
     5281 cattgttaaa caaaagtgac agtgaatttt caaaagaatt gtcaaatgtt aagaagcaat
     5341 taaaagataa gtctaaagtt tcggtaacta ctactctatt tagtaaaaaa aagaactata
     5401 ctaaaaaaag taacagtgaa aatgtaataa aaatggcaga agaaataaaa aaagataaag
     5461 agataccaaa cggtatagag cttagtataa aattttcgga caataaaata aatacggtta
     5521 aaccaaattt taacggtgaa agcacttcag aatatggtgt gtttgatcaa gaataaaatt
     5581 aatgatgaaa atttaacgga gaatagtgta tattgagtag atcaagaata aaaagataat
     5641 tctactattg ttgtgaaggc aaataagtag aagattttaa gtgtaatttc tggtgattta
     5701 aataataata taaatggaag tactgatata aaacttttta acctactaga ttcttataat
     5761 ttgctttcca ttttatgacg atttttactc aattgagtga tagaatcaaa aaagccatct
     5821 caaaaattaa tcaagcaaac aacattccaa acaatgctcg caaatcacca atgtatcact
     5881 ctccaattac gtaactatga tttaatttaa gcatagttat tgaggttttg tgatatatag
     5941 tataaaatta atgagaatta aatttaataa tgtaaaattc atcttcgggg tcgggtgtaa
     6001 ttcccaaccg gcagtaaata aagcctgcga cctgctagta tgtatcatat tagtggctga
     6061 tctagtgaga ttctagagcc gacagtatag tctggatggg agaagatgga ggttttttgt
     6121 tgtgcaataa tcctcctatt cttacgagat gaatggaagg agaaaattga atatgcaaca
     6181 aaataaacgt cttatcacaa taagtatgtt gagcgcgatt gcgtttgtgt taacttttat
     6241 caagtttcct ataccatttt tgccaccata cttaacttta gattttagtg atgtaccgtc
     6301 actactagct acatttacgt ttggaccagt tgccggtatc gtagttgcac tggttaaaaa
     6361 tttattgaac tacttattta gtatgggcga tccagttgga ccatttgcta actttttagc
     6421 aggcgcaagt ttcttattaa ctgcttacgc catctataaa aataaacgtt caacaaaatc
     6481 tttgattact ggattaatca ttgcaacaat cgttatgact atcgtgttga gtattttgaa
     6541 ctatttcgtt ctattacctt tgtacggtat gatatttaac ttagctgata tcgcaaataa
     6601 tcttaaagta atcattgttt caggaattat accattcaat attattaaag gtatcgttat
     6661 ttctattgta tttattttac tatatagaag gcttgcgaat ttcttgaaaa gaatttaatc
     6721 aaattaaagc aaaataatat acacataata ataaaaagca ggtgactatc aataaacgat
     6781 agcttgcctg ctttttctat agaacatttg tctaaaaaat caattattca aattttaaag
     6841 cgtccccatc aaatgattcg tctgcaattt taatagaatc tgtagggcat ccatcaattg
     6901 catcttccat atcttcatat aattcctcag gtacttctgc agtaccttgg ttatcgtcaa
     6961 ggattacgaa agcaatacct tcgtcgtcgt aatcatatat atctggcgct gctgcaccgc
     7021 atgcaccaca tgcaatacaa gtatccatat caacgattgt atattttgcc aatgtcttcg
     7081 cctcctttga taaaaatgct aaaatagtaa tgtgactaaa attttagaca gcatcatttt
     7141 tattttcaaa ttatccgttt tacagagtga gggttaaatt tgcaacacat tataaaaaca
     7201 gcattacaac aaacatttaa ctataaaaca aataaaagta tttacaatat cttagttggt
     7261 aagaaatctc accaaacctt ttttgacgct tgtagtcaac aacagttgtc attatatcac
     7321 agtttaccac tattaaaata tccgtctttt gagctatttt tagaaaaaat caatgaattt
     7381 aatgctgaaa tggaaatcat gttgcatcct agatatacat ttgaaagcat ggggcaaaca
     7441 tttcaagcaa ttcaactatt agtgcaaacc atgtctaata ccaaacaaca tgtttttcat
     7501 tttgtaccaa tctctcaaaa taataagata caagaaactg taaagattgt ctataactac
     7561 attaaagaaa accaattaca aattgatttt gaaaatgaac tacataactt gtttaaagca
     7621 ataactttaa aagggccatg ttacttacat tattatttgc aaggctatga tgaaccaatg
     7681 tatacgagac agcaagttag tttaatagaa aagctatctc aacagcaatt gtttgaatac
     7741 gaaatgaata atttagtgac aatgatgttt gaattagaaa gtggagaata tactatttta
     7801 tcaaaaataa taatgaaacc tacattatta aatcaaactt atattactta tacaaaattg
     7861 cttgaacaat tcacgatgga agatatagcg gctcaacaac aagttaaaat caatactatc
     7921 gaagatcatg tacttgaaat cttaatcaaa ggttacatgt ctaattacga tgattatgtt
     7981 gaactagaag atcaactcca gtttttgaat ttttatcaac agcatcgtgg cgaacgatta
     8041 aaattttaca aagaacaatt tgacacgtta tcatattttc aattaaaagt attaatcgtt
     8101 ggatttgaaa gaggtgatct gaatgttgca tgatatttta cgaaacaaat ttggattcga
     8161 gagttttaaa ccgggacaac aggaaattat agaaagtata atgtctcaac aacacactct
     8221 aggtatactt ccaactggaa gtggaaagag tttgtgttat caaataccta cgtatttatc
     8281 aggtaagccg acattaatta tctcaccgtt aatatcttta atggatgacc aagttatgca
     8341 gttgaaaata aatggagaaa aacgtgtaac atgtattcac tctggtatgg atgaaattga
     8401 gaaaaagcat aatattaaat gtttacgaca tagccgcttc atctttctaa gtccagaatt
     8461 tctcctgcaa ccgtcaaatt ttaaattaat atctatgata gactttggca tgattgttct
     8521 agatgaagca cattgcctat ctgaatgggg atatgatttc agaccacatt atgctctaat
     8581 aggaaaagta acaaagcatt ttaaagaagc ggttgtctta gcattgacag caactgcacc
     8641 accgcattta caagatgatt tgacggaaat gttagcgatt caattcaatg ttattaaaac
     8701 tacaatgaat cgcccaaata taagctttaa gcatcttaat tttcatgatg atgaagataa
     8761 aattgaatgg ttgctgccgt ttctacaaca gtcgggacca acgattattt atgtctcatc
     8821 gaaaaagatg tgtctgaatt tagcgcaact tatttatgat tcaggttttc ttacaggtat
     8881 ttatcatggt gatatgaatt atcaagagcg acacacagtt caacaacaat ttttaaataa
     8941 tgatattccg attatagtcg caacgagtgc ttttggtatg ggaattaata aaaaagatat
     9001 tcgcacaatc attcactttc atctttcaac aagtccttct aactacattc aagaaattgg
     9061 ccgtgcgggt cgcgatggtg aactaagtca ggcaattagt ttattccaac cggacgataa
     9121 atatatttta gaaacgttat tatttgcaga tatgataaca gaagaagatg tacaaaattt
     9181 cgaaatagga gaatttttag ctcccgataa acaagccgtt ttgacaacgt tgcaatcatt
     9241 ctatagtatc ggcgccttga aacagatatt taagcaatca tttaaacgaa agcaattagg
     9301 attctttcgc atgattggct attgcaaatt ggatcaatgt agacggaagt atttattaga
     9361 atttttcggt gaatatccac cggcacaaga tcgatgttgt gacaatgatt ctaatataac
     9421 tgatatcgca attttaaata agaagaaggt aattagaagt attggatttg atgaaaagtt
     9481 gcaaaattta tttctcagat agtattactt tactaaaaga aaattgacaa gctataatta
     9541 gtgtatacac aattgaaaaa tgattgaaat aattttgaaa aatatacata aacatatgtc
     9601 atgtgggtat attttatgta aaatcattgt aatagaatag aaaggaagat ggctatgtct
     9661 aataatttta aagatgactt tgaaaaaaat cgtcaatcga tagacacaaa ttcacatcaa
     9721 gaccatacgg aagatgttga aaaagaccaa tcagaattag aacatcagga tacaatagag
     9781 aatacggagc aacagtttcc gccaagaaat gcccaaagaa gaaaaagacg ccgtgattta
     9841 gcaacgaatc ataataaaca agttcacaat gaatcacaaa catctgaaga caatgttcaa
     9901 aatgaggctg gcacaataga tgatcgtcaa gtcgaatcat cacacagtac tgaaagtcaa
     9961 gaacctagcc atcaagacag tacacctcaa catgaagagg aatattataa taagaatgct
    10021 tttgcaatgg ataaatcaca tccagaacca atcgaagaca atgataaaca cgatactatt
    10081 aaaaatgcag aaaataacac tgagcattca acagtttctg ataagagtga agctgaacaa
    10141 tctcagcaac ctaaaccata ttttacaaca ggtgctaacc aatcagaaac atcaaaaaat
    10201 gaacatgata atgattctgt aaaacaagat caagatgaac ctaaagaaca tcataatggt
    10261 aaaaaagcag cagctattgg tgctggaaca gcaggtgttg caggtgcagc tggtgcaatg
    10321 gctgcttcta aagctaagaa acattcaaat gacgctcaaa acaaaagtaa ttctggcaag
    10381 gcgaataact cgactgagga taaagcgtct caagataagt ctaaagatca tcataatggc
    10441 aaaaaaggtg cagcgatcgg tgctggaaca gcaggtttgg ctggaggcgc agcaagtaaa
    10501 agtgcttctg ccgcttcaaa accacatgcc tctaataatg caagccaaaa ccatgatgaa
    10561 catgacaatc atgacagaga taaagaacgt aaaaaaggtg gcatggccaa agtattgtta
    10621 ccattaattg cagctgtact aattatcggt gcattagcga tatttggagg catggcatta
    10681 aacaatcata ataatggtac aaaagaaaat aaaatcgcga atacaaataa aaataatgct
    10741 gatgaaagta aagacaaaga cacatctaaa gacgcttcta aagataaatc aaaatctaca
    10801 gacagtgata aatcaaaaga ggatcaagac aaagcgacta aagatgaatc tgataatgat
    10861 caaaacaacg ctaatcaagc gaacaatcaa gcacaaaata atcaaaatca acaacaagct
    10921 aatcaaaatc aacaacagca acaacaacgt caaggtggtg gccaaagaca tacagtgaat
    10981 ggtcaagaaa acttataccg tatcgcaatt caatactacg gttcaggttc accggaaaat
    11041 gttgaaaaaa ttagacgtgc caatggttta agtggtaaca atattagaaa cggtcaacaa
    11101 atcgttattc cataatataa ctatataaat tgtaactgaa ctgctatata caaacgtgca
    11161 tattataagt atctctaaat attttggaga acttaaattg cgcttataag tatgtagcgg
    11221 ttttttcatt tttcaaagtt tgttatttaa caaggtcttt tctcgaatat tggcatatca
    11281 atttaacttt ttaaatagtc atcaaaaaga taaaacacca caatcaacaa atttaacgag
    11341 gaagaataaa aaataaatca acatattaaa ttgtagtgtt attcaactcc gtagctaaca
    11401 attctctatg cacattaaac aaattgtcaa aaatatatca taaatcttca agcacagact
    11461 tagcgcatca atcactgaac tgttataata gtttgggatt aaaggaggcc gaaacaatgc
    11521 aaaaagttga aagtatcata attggtggag ggccatgcgg attaagtgcg gctattgaac
    11581 aaaaaagaaa aggtattgat accttaatta ttgaaaaggg taatgtcgtt gaatcaatct
    11641 acaattatcc tactcaccaa acatttttct catcaagtga taaattaagt attggggacg
    11701 taccgtttat cgttgaagaa agtaaaccaa gacgtaatca agcgctagtt tattaccgag
    11761 aagttgtaaa acatcatcaa ttaaaagtaa atgcatttga agaagtatta actgttaaaa
    11821 aaatgaataa taaatttact attactacga cgaaagatgt ttatgaatgt cgatttttaa
    11881 caatcgcgac aggctattat ggtcagcata atacattaga agttgaaggt gcggatttac
    11941 ctaaagtgtt ccattatttt aaagaggcac atccgtattt tgatcaagat gttgtaatta
    12001 tcgggggtaa gaattcggct atcgatgctg ctttggagtt ggaaaaagct ggtgctaacg
    12061 tgacggttct atatcgtggt ggagattatt cgccttcaat taaaccgtgg atacttccaa
    12121 atttcacagc attagtaaat catgaaaaaa ttgacatgga atttaatgct aatgttaccc
    12181 aaataactga agatactgtg acttatgaag taaatggtga aagtaaaacg atacacaatg
    12241 attatgtatt tgcgatgatt ggttatcatc ccgattatga atttttaaaa tctgtaggca
    12301 ttcaaattaa tacaaatgaa tttggaacag cgcctatgta taataaagaa acatacgaaa
    12361 caaatatcga aaattgctat attgcaggtg taattgctgc agggaacgat gcgaatacca
    12421 tttttattga aaatggtaaa ttccacgggg gcattattgc tcaaagcatg ctagctaaga
    12481 aacaaacgcc cttagaatca taaaaataaa ggtctatgta ctaaataact tagttttaca
    12541 acgactgaca ttcatgatat gtcaaatgag gttgatgact aatgattgta catagacctt
    12601 tttatgttac gtattcatta taattcaaaa tatgatttga tttcagcttt atctaaattg
    12661 ttgcttaacg cgactaataa ttttaatctt gcttttggac cattcaagcc gttagaaaaa
    12721 ataaaacctt gttgtgcgag ttggtaacca ccaccatcgt atgcgtaagt tggactcaca
    12781 ataccattaa aggaacgtga aactagcaca ataggtatat ttaaagatac taattgttga
    12841 atgccttcta atgcgcttgg aggtatgttg ccttgtccta acgcttcaat aaccatacca
    12901 tcgatacctt ctcgactata aaaactaaaa atgtcacctg gcatacccat atatgctttt
    12961 actaatggta catttagttt gtcattgaca ttttccaatg cttgttggcg atatggcata
    13021 tgatggaatt gcacacgatc ctttgtcaat acacctagcg gaccatgatt tggactttga
    13081 aatgtgtttg tattagacgt atgtgttttg gtaacattac gcgccgtatg aatttcatca
    13141 ttaaatacaa ccatcacgcc tttatgacgg gccttttcat cagaggcaac gcgaatagcg
    13201 gaaatataat tatataatcc gtcagaacca atttcattag acgagcgcat tgcgccagta
    13261 ataacaacag gttgctcgat acctaatatt aaatcaagta aaaaggcagt ttcttctaac
    13321 gtatcggtac catgcgtgat aacgaaacca tcataatatt tatttgttac tgcttctaaa
    13381 ataatatcct ttaattgttt aacatgttgg attgtcatat gaggtgatgg tacattaaaa
    13441 ggatttaatt catctatttg tgcatattga tttatgacat cttgatgcat tgaaataggg
    13501 ttgatatcat ttgttactac tttattagat tggtcttgtg acatactaat ggtgccacca
    13561 gtatgaataa caagtagatg tttcatatat ttcctcctat atttaattta cctaattatg
    13621 ataaaatatt attcataaaa cgacaaggaa gggaaatgac gcatgaaagc cattaatatt
    13681 gcattagatg gtccagctgc tgccggaaaa agtacaattg cgaaacgtgt agccagcgaa
    13741 ctatcaatga tttatgtcga tacaggagca atgtatcgtg cattaacata caaatattta
    13801 aaattaaaca aaactgagga ctttgcaaaa ctagttgacc aaacaacatt agatttaact
    13861 tataaagcag ataaaggtca atgtgtcatt ttagataacg aagatgtaac agacttttta
    13921 agaaataatg atgtgacgca acatgtttca tacgttgcat ctaaagagcc agtacgttca
    13981 ttcgccgtta aaaaacaaaa agagttagct gcagaaaaag gtatcgtaat ggatggtcgc
    14041 gatatcggaa ctgtagtgct accagatgca gatttaaaag tatatatgat tgcatcagtt
    14101 gaagagcgag cagaaagaag atataaagat aatcaattaa gaggtatcga atcaaatttt
    14161 gaagatttaa aacgtgatat tgaagctcgt gatcaatatg acatgaaccg tgaaatatca
    14221 ccattaagaa aagcagatga tgcagtgaca ttagatacga caggcaagtc gattgaagaa
    14281 gttactgacg aaattttagc gatggtgagt caaattaaat aatctaataa gtcataacta
    14341 tctatttgta atgtgcaacg cttaacgcat atacaaaatg aatgtgctga taatgattta
    14401 ctcaaattaa aaggtgattt ttattcaatg atgaatgaaa gttgcctttt tatttttggt
    14461 aaaagttaat gcgtcagtga attgtgtaag tttttcaaaa agtaaaaaga aataataaag
    14521 gtgaattatt agaattccaa aaataattca ttacattcat aaagcatttt acaaatggta
    14581 agaaaatgag tgttacaaat ctaaatattg caaaagaagc tgatttagtc acaaaaaatg
    14641 tcctatgtaa taattcgaga aagatgcact atatacggtc ttcttactat tcaaatgtaa
    14701 aagttgctta tttgcgtggc tttttgtttt ataaaagtat aaaattttac tataatatat
    14761 cttgtagaga acaatgaaat gacagactta taaaacaaaa tagtaagcta tgctaatttt
    14821 tatacaaaaa atatgtataa acatagcaaa ctgtattact ttgatacaaa aatggttgta
    14881 ataaatattt atcgatatga cgacttgaat atgataaagt gacatattta tgtatatgac
    14941 tatttcgcaa aatgtaatcg aggtagaatt tcttgacaat tctgtcagtt tataagatgt
    15001 tataaatatg tagtgtataa ggaggcaaac aagatgactg aagaattcaa tgaatcaatg
    15061 attaacgata ttaaagaagg tgacaaagtc actggcgagg tacaacaagt tgaagacaag
    15121 caagttgttg ttcatatcaa cggtggtaaa tttaatggga ttattcctat tagtcaatta
    15181 tctacgcatc atattgatag cccaagtgaa gttgtaaaag agggcgacga agttgaagca
    15241 tatgtcacta aagttgagtt tgatgaagaa aatgaaactg gagcttacat cttatctaga
    15301 agacaacttg aaactgagaa gtcttatagt tatttacaag aaaaattaga taataatgaa
    15361 atcatcgaag cgaaagtaac agaagtagtt aaaggtggtt tggttgttga tgtaggacaa
    15421 agaggttttg ttccggcttc actaatttca acagacttca ttgaggattt ctctgtgttt
    15481 gatggacaaa caattcgtat taaagttgaa gaattggatc ctgaaaataa tagagtcatt
    15541 ttaagccgta aagcagttga acaagaagaa aacgatgcta aaaaagatca attattacaa
    15601 tctttaaatg aaggcgatgt tattgatggt aaagtagcgc gtttaactca atttggtgca
    15661 tttatagaca ttggcggtgt tgatggttta gtgcatgtat ctgaactttc tcacgaacat
    15721 gttcaaacac cagaagaagt agtttcaatt ggtcaagatg ttaaagttaa aattaaatct
    15781 attgatagag atacagaacg tatttcatta tcaatcaaag atacgttacc aacacctttc
    15841 gaaaatatta aaggtcaatt ccacgaaaat gatgtcattg aaggtgtcgt agtaagattg
    15901 gcaaactttg gtgcatttgt tgaaattgca ccaggtgtac aaggacttgt acatatttct
    15961 gaaattgcac acaaacacat tggtacgcca ggtgaagtgt tagaacctgg tcaacaagta
    16021 aatgttaaaa tattaggtat tgatgaagag aatgaaagag tatcactatc tattaaagca
    16081 acattaccaa acgaagatgt tgttgaaagt gatccttcta cgactaaggc gtacttagaa
    16141 aacgaagaag aagataatcc aacaattggc gatatgattg gtgataaact taaaaatctt
    16201 aaactataat ttaatattta atagtcaact ccacatgttt atgattgcat gtggagtatt
    16261 tttatgtaac aaaatatact cggaatgata acgtggacaa atttaactaa gtgtttaaaa
    16321 agataaagtt ttaagtgctg atttttatca ttacagtaat aaactcattt tgaatacaca
    16381 gtctcatgtg atattattaa aaagatataa gaaagagagg aagttagctt atgactaaac
    16441 ctatagtagc tattgtaggt aggcctaatg taggtaaatc tacaattttt aatagaatag
    16501 ttggagaacg tgtttcgatt gtggaagaca cgccaggtgt aacacgagat cgtatttatt
    16561 cttcaggtga atggttaaca catgatttca atattattga tacaggtggt attgaaattg
    16621 gtgatgcacc attccaaaca caaattagag cgcaggcaga aatcgccata gatgaagcgt
    16681 atgttattat ttttatggtt aacgtgcgtg aaggattgac acaaagcgat gaaatggtcg
    16741 ctcaaatttt atacaaatct aaaaaaccgg tcgtattagc ggttaacaaa gtagataata
    16801 tggaaatgcg tacagacgtg tatgatttct attcattagg atttggtgaa ccgtatccga
    16861 tatcagggtc acatggttta ggtcttggtg acttgttaga tgcagttgtt tctcattttg
    16921 gtgaagagga agaagatcct tatgatgaag atacaattcg actatccatt attggacgac
    16981 caaacgtagg taaatcaagt ttagtaaatg ctattttagg tgaagatcgc gttatcgttt
    17041 ctaatgttgc agggacaacg agagacgcta ttgatacaga gtatagttat gatggacaag
    17101 attatgtttt aatcgatact gctggtatgc gtaaaaaagg aaaagtatat gaatcaactg
    17161 agaaatattc agtattaaga gctttaaaag cgattgaacg ttcaaatgtt gttttagtgg
    17221 tcatagatgc agaacaaggc atcattgaac aagataaacg tgttgcagga tatgcacatg
    17281 aacaaggtaa agcagtcgtg attgtcgtaa ataaatggga tactgtggaa aaagatagta
    17341 aaacgatgaa gaaatttgaa gatgaagtac gtaaagaatt ccaattttta gattatgcac
    17401 aaattgcttt tgtgtctgct aaagaacgca caagattacg tacattattc ccttacatca
    17461 atgaagcaag tgaaaaccat aaaaaacgtg ttcaaagttc aactttaaat gaagttgtta
    17521 ctggtgcaat ttccatgaac cctacaccaa cagacaaagg tagacgtttg aatgtctttt
    17581 atgcaacaca agttgctata gaaccaccga catttgttgt atttgttaat gatgtagaat
    17641 taatgcattt ttcttataaa cgctatttag agaatcaaat ccgtgccgct tttggttttg
    17701 aaggtacacc aattcatatt atagctcgaa agagaaatta acgattgggg gataacaatg
    17761 actaaaatta ccgtttttgg tatgggaagt tttgggacag cccttgccaa tgttcttgca
    17821 gaaaatggac atgatgtttt gatgtggggt aaaaatcaag atgctgttga tgaattaaat
    17881 acatgtcata caaataaaaa gtatttaaaa tacgcgaaat tagatgttaa catcatcgct
    17941 acttcagata tgaccaaagc aattcaattt gcagatattt acttaatggc tttacctact
    18001 aaagcaatgc gagaagttgc ttctcaaatt aatgataagc tgacctctaa aaagactttt
    18061 atacatgttg ctaaaggtat tgaaaatggg acgtttaaac gtgtgtcaga aatgattgaa
    18121 gattctattt cacctgaata taatgcaggt attggcgtgt tgtcagggcc aagtcatgcg
    18181 gaagaagttg tagtcaagca accaactaca gttgctgctt catcaaaaga taaaagtgta
    18241 agtaaattaa cgcaagattt atttatgaat gattatttgc gtgtgtacac gaatgatgac
    18301 ttgattggtg ttgaacttgg tggtgcattg aaaaatatca tcgcagtagc aagtggtatc
    18361 gtagctggaa ttggctacgg tgataatgca aaagctgcat taatgactcg tggcttagcg
    18421 gaaattagta gattaggtga aaagttaggt gccgatccta tgacatttct aggtttaggt
    18481 ggtatcggtg acttaatcgt tacttgcaca tcaacacatt ctcggaattt cacattagga
    18541 tataaacttg gacaaggtga atcaatggat caagcattat ctgaaatgaa tatggttgtt
    18601 gaaggtattt atacaactaa atcagtttat catttagcta aagaaaaaaa tgtggatatg
    18661 ccaattacaa atgcattata tagagtatta tttgaaaata tctcagtaaa agaatgcgta
    18721 aaagatttaa tggagcgcga taaaaaatct gaataaaatg tgtattatta cacatatacg
    18781 gctaataatt acgtaaaatg aatagaaatg gcttaaattc aacgtttttg ccacataaat
    18841 gattgcgatt tatgtaatgc ttgtgttaaa gttttaccat aatgatataa acatatcatt
    18901 atcaaacctc aggaggtgaa tgtctaatga acaaaacaga tttaatcaat gcagttgcag
    18961 agcaagctga tttaactaaa aaagaagctg gttcagcagt agatgctgta ttcgaatcaa
    19021 tccaaaactc acttgctaaa ggtgaaaaag tacaattaat tggtttcggt aactttgagg
    19081 tacgtgaacg tgctgcacgt aaaggtcgta accctcaaac tggtaaagaa attgatatcc
    19141 cagcaagtaa agttccagca ttcaaagctg gtaaagcatt aaaagatgct gtaaaataat
    19201 tttacttaaa aagccctgaa taagggcttt ttattttgct tttaatactt acaacctgta
    19261 cataaattgt aatgttcttc taagttttta atctttggaa cattcattgt ttaagcgttc
    19321 aaaatattac atgacataat agttttgata aaaatagcaa ctacaaaata tacatatgaa
    19381 tattgagatt aattgttagc gttgaattta cttaaaaggt aaccatgtct actatagtat
    19441 tttacgttat ttaaaaagat gaataatgta aatgaagtaa aggttattat gagaattaca
    19501 aaagctacat aaattactgt tagtttaaat tgaaatttaa aaatgataag taatcttaat
    19561 gcttcaaaag attttaattt cttacatata atgttaatat gaatagtgat aatatcgtga
    19621 ggtgtagaca tggaaacaac tgttagcaaa ttggaaagac aaatagaaga aagattaaag
    19681 ggcgtatcag aatatgaatc cattaatatt aatcatcggt taggtaagtt acttgattta
    19741 tatgatattc cagatgttgc caaagtggca tgcctcacaa ttgacacatc aatgagacac
    19801 cttgatgaca ttacatataa tcatttgtct aaacattcca ttttgattgg agatttaatt
    19861 agtgcacatt tttatacgct actagcagaa atcaatgatt tatcatttca aaatgaaatt
    19921 agtaaagcaa ttgttgaaat taatgaattg aaatcttcat tacatcatca agctttaaat
    19981 gattatgaaa tttctcaagc aatagtaaaa attgaaacac ttttcccata tattacgtta
    20041 tctcatttcg gtatcaatat tgatgagtcg gaaatatata attatttatt tgaagatatg
    20101 tctgattact atccatcata cttcaagaaa tataatcaaa gtgaagtaaa gcattactta
    20161 cacgatatcc aaaaaagtta tttaaaaagt agaggtaatt aagcatggct gacaataaag
    20221 caaataaaga gcaagtacat cgcgtttttc aaaatatttc aaaaaaatat gatagattaa
    20281 ataatattat tagttttgag cagcataaag tatggagaaa acgcgtcatg aaagacatgg
    20341 gtgttagaaa agggacgaaa gcattagatg tttgttgtgg tactggtgat tggacaatcg
    20401 cattaagcaa agctgtagga ccaacaggtg aagttactgg tattgacttt agtgagaata
    20461 tgttagaagt aggaaaagaa aaaactgctt caatggaaaa tgttaaactt gttcatggtg
    20521 atgcaatgga attgccgttt gaagacaatt cttttgatta tgtaacaatt gggtttggat
    20581 taagaaatgt gccagactat ttagtcgcgt taaaagaaat gaatagagta cttaaaccag
    20641 gtggtatggt ggtatgtctt gaaacgagcc aaccaacttt gccagtcttt aaacaaatgt
    20701 atgcactata ctttaaattt gttatgccta tttttggaaa attatttgca aaatcaaaag
    20761 aagaatatga atggttacag caatctacgt ttaattttcc tggaaaagaa gagttgaagc
    20821 gcatgtttga agaagcaggt ttcataaatg taagagtacg tagttttaca gggggcgttg
    20881 ctgcaatgca ccttggctat aaagaaaaag ataataccaa aggtgattaa cgtggcaaag
    20941 ttaaacatga acaatgaaat taagaaagtg gaacaacgac ttgaaaaggc aataaaaagt
    21001 aaagattctg tattagaaca ggcatcatta cacttattgt cttctggtgg taaaagagta
    21061 cgcccagcat ttgttattct gagtagccaa tttggcaaag atgaacaaac gtctgaacaa
    21121 acgtatcaag ttgcagtcgc attagagtta attcatatgg caacacttgt tcatgatgac
    21181 gttattgata aaagcgacaa gcgtcgaggc aagttaacca tatcaaagaa atgggatcag
    21241 acaactgcta ttttaactgg gaatttttta ttggcattag gacttgaaca cttaatggcc
    21301 gttaaagata atcgtgtaca tcaattgata tctgaatcta tcgttgatgt ttgtagaggg
    21361 gaacttttcc aatttcaaga ccaatttaac agtcaacaga caattattaa ttatttacga
    21421 cgtatcaatc gcaaaacagc actgttaatt caaatatcaa ctgaagttgg tgcaattact
    21481 tctcaatctg ataaagagac tgtacgaaaa ttgaaaatga ttggtcatta tataggtatg
    21541 agcttccaaa tcattgatga tgtattagac ttcacaagta ccgaaaagaa attaggtaag
    21601 ccggtcggaa gtgatttgct taatggtcat attacgttac cgattttatt agaaatgcgt
    21661 aaaaatccag acttcaaatt gaaaatcgaa cagttacgtc gtgatagtga acgcaaagaa
    21721 tttgaagaat gtatccaaat cattagaaaa tctgacagca tcgatgaggc taaggcagta
    21781 agttcgaagt atttaagtaa agctttgaat ttgatttctg agttaccaga tggacatccg
    21841 aaatcactac ttttaagttt gacgaaaaaa atgggttcaa gaaacacgta gtatttatga
    21901 aaagtattga aagcgcttta ccaacctgtt aatatataat agtaatatac ataaacctat
    21961 taaacacagg gggatacata aagtggaacg tacattttta atgattaaac cagatgcagt
    22021 acaaagaaat ctaattggtg aagtaatttc aagaattgaa agaaaaggac taaaacttgt
    22081 cggtggtaaa ttaatgcaag taccaatgga acttgctgaa acacattatg gtgaacacca
    22141 aggtaaacca ttttataatg atttaatttc atttattaca tcagcaccag tgttcgcaat
    22201 ggtagttgaa ggtgaagatg cagttaatgt atctagacat attattggca gcaccaatcc
    22261 ttcagaagct tcaccaggat caattagagg tgatttaggt ttaactgttg gtagaaatat
    22321 cattcacggt tcagattcat tagagtctgc tgaacgtgaa attaatctat ggtttaatga
    22381 aaatgaaatt actagctatg cttcaccacg tgatgcatgg ttatatgaat aaaatataaa
    22441 ctgtaaacct ttacgattta tttataaagg tagaaagggt tttgttatgt ggttagtcat
    22501 tatgattata cataacaagg cccgtttttt atgttgtagt aaattacttg aaaaatttta
    22561 tagttttttg gtaacacgta ttaaaaagag aggaatattc tttatcaaat gaaactaaac
    22621 agagagaagg ggttgttaaa atgaagaata ttatttcgat tattttgggg attttaatgt
    22681 tcttaaaatt aatggaatta ctatatggtg ctatattttt agataaacca cttaatccta
    22741 taacaaaaat ta
//
LOCUS       DALAAR010000043        22509 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000043 DALAAR010000000
VERSION     DALAAR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.045"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            372..1433
                     /locus_tag="O2458_001710"
     CDS             372..1433
                     /locus_tag="O2458_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ3887575.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1691..3148
                     /locus_tag="O2458_001711"
     CDS             1691..3148
                     /locus_tag="O2458_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCZ3887576.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3141..3863
                     /locus_tag="O2458_001712"
     CDS             3141..3863
                     /locus_tag="O2458_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887577.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            4014..5141
                     /gene="queG"
                     /locus_tag="O2458_001713"
     CDS             4014..5141
                     /gene="queG"
                     /locus_tag="O2458_001713"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCZ3887578.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5146..5616
                     /gene="trmL"
                     /locus_tag="O2458_001714"
     CDS             5146..5616
                     /gene="trmL"
                     /locus_tag="O2458_001714"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCZ3887579.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5775..6374
                     /locus_tag="O2458_001715"
     CDS             5775..6374
                     /locus_tag="O2458_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCZ3887580.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6399..6551
                     /locus_tag="O2458_001716"
     CDS             6399..6551
                     /locus_tag="O2458_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887581.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            7328..7723
                     /locus_tag="O2458_001717"
     CDS             7328..7723
                     /locus_tag="O2458_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887582.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            7919..9304
                     /gene="fumC"
                     /locus_tag="O2458_001718"
     CDS             7919..9304
                     /gene="fumC"
                     /locus_tag="O2458_001718"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCZ3887583.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(9767..10588)
                     /locus_tag="O2458_001719"
     CDS             complement(9767..10588)
                     /locus_tag="O2458_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ3887584.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            10751..11863
                     /locus_tag="O2458_001720"
     CDS             10751..11863
                     /locus_tag="O2458_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCZ3887585.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            11885..12508
                     /locus_tag="O2458_001721"
     CDS             11885..12508
                     /locus_tag="O2458_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3887586.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            12864..13328
                     /locus_tag="O2458_001722"
     CDS             12864..13328
                     /locus_tag="O2458_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887587.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            13507..14631
                     /locus_tag="O2458_001723"
     CDS             13507..14631
                     /locus_tag="O2458_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCZ3887588.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            14700..15044
                     /locus_tag="O2458_001724"
     CDS             14700..15044
                     /locus_tag="O2458_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCZ3887589.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            15427..15525
                     /locus_tag="O2458_001725"
     CDS             15427..15525
                     /locus_tag="O2458_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887590.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            15816..17012
                     /locus_tag="O2458_001726"
     CDS             15816..17012
                     /locus_tag="O2458_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ3887591.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            17002..19938
                     /locus_tag="O2458_001727"
     CDS             17002..19938
                     /locus_tag="O2458_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ3887592.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            19935..20876
                     /gene="yhaM"
                     /locus_tag="O2458_001728"
     CDS             19935..20876
                     /gene="yhaM"
                     /locus_tag="O2458_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCZ3887593.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(20997..21959)
                     /locus_tag="O2458_001729"
     CDS             complement(20997..21959)
                     /locus_tag="O2458_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCZ3887594.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(22164..>22509)
                     /locus_tag="O2458_001730"
     CDS             complement(22164..>22509)
                     /locus_tag="O2458_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ3887595.1"
                     /translation="FFLPYRKSFKVHKLTKRKWLIFYNTYVNQPVHKFYFCINLILPL
                     IILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFSNYGQYIEEHSTGI
                     NILKKIKNPYHL"
BASE COUNT         8199 a         3097 c         3932 g         7281 t
ORIGIN      
        1 tttaacttct ttttttggta ttacattaat gataatgatt atattatctg gggttatata
       61 tttgtttcaa cttttctaat aatatatttg taaaagtgct attttattgt gctttatacc
      121 aagtcgattc aatttatcta tcttaatagt aatatggcta ttttaatatt tacaatttat
      181 tacattatta gtcacaggat gtaattagta gtattttaaa attaacatga cattgattag
      241 tgagtagatt gtttaaattt gctttattca atttgtggat tgttatattt agattaaatt
      301 agccaactaa tcattgttaa agttaatttt ctataatatg atgagtttaa tgttcaaatt
      361 ggaggtcgtt tatgagtaac gcaaataatg ctgataataa acagtttttc agtaagattt
      421 taaattctat tggtgctggt gttgttattg cacttgttcc gaatgcttta ttaggtgaag
      481 tattaaaaat atttaaaagt ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac
      541 taattcaatc atttatggca tttattattg gggttttagc tgctcatcaa tttaaattta
      601 atggtgcagg tgctgctata gtaggtacat cagctatgat tggttcaggt gctgttgtat
      661 atagcaataa tagttttatg ttaaaaggta taggtgacat tataaatacg agtttggttg
      721 ttattattgc atgtcttata tatatggtat tacaaaacaa acttggatct tttgaattaa
      781 ttatattacc agttttagta cctatcgtta gtgggggtat cggtctaatc acattacctt
      841 acattcgaaa aattactcaa gcaattggaa atgtaattca ttcttttaca gacttaaatc
      901 cgttgttaat gtctatactt attagtgtgg cattttcatt attaatggta acaccaatat
      961 cgttagtagc gattgcaact gcgattagtt taaacggact aggaagtgga gcagcaaacc
     1021 taggtatagt ggctgcctgt gttactttct tatttggctc attgcgtgtc aattctattg
     1081 gtgtaaatgc agttttactc ataggagctg caaaaatgat gatcccagtt tacttgaaaa
     1141 atttaattat ttcgatacca ttgactataa atggaataat tactggaatc attgcttacg
     1201 ttcttcaggt taaaggaaca ccattgtctg ctggatttgg atatacagga ttagttggcc
     1261 caataaatgc atttaaccga atgtcaggtg atccaacaat gaatattatt ttattagcac
     1321 ttggttattt tgtcatacca tttgtatcgg catttattgt tcatgaattg tgtaaaaaat
     1381 tcattcctat atattctaat gatatttata aatttgaagt tccaaaacaa taatttatag
     1441 cttgatgaca agaataagtt ttaatatatg ataaatatag gcgttactgt caataagata
     1501 ttgatagtag cgcttttttg attgatttta aaatgtgaga tttgttcacg tataatagcg
     1561 gattttttat gtacagtctt ttaaaaagaa gcttttcttt atattgataa aaatacattt
     1621 ataaaacact attgcatcaa aatgtattaa tatgtataat tatgaatatt aattagggag
     1681 ggctgtataa atgaagtgtt taattaggtt tatattagta ttagggttgt taattagcag
     1741 tgctatggtg tatatcaatc ccacagcaca tgctgaacaa gatcaaacat gggagaagat
     1801 taaagaacgc ggtgaactta gagtgggtct ttctgcagat tatgcaccaa tggaatttga
     1861 gcatacagtt aatggtaaga ctgagtatgc aggtgtagat attgatttag ctaaaaaaat
     1921 tgcgaaagat aataatttaa aattaaaaat cgtcaatatg tcatttgata gtttgttagg
     1981 agctcttaaa actggaaaaa ttgatattat tatttccgga atgacttcaa cgcctgaacg
     2041 taagaagcaa gttgattttt cagattcata tatgatgact aaaaatatca tgcttgtaaa
     2101 gaaagataaa gttaatgaat ataaagatat caaagacttt aataataaaa aagtaggggc
     2161 acaaaaggga actgaacaag aaaaaatcgc tcaaaccgaa attgaaaatg catctattac
     2221 ttcattaagc cgtttaccag acgttatatt agcattgaaa agtggaaagg ttgaaggtgc
     2281 tgtagttgaa aaacctgtag cagaagcata tttaaaacaa aatcctaaat taggaatttc
     2341 caatgtgaaa tttaatgaag aagaaaaaga tacagtgata gcagtgccaa aagattcacc
     2401 aaaattattg tcacaaatta ataaaacgat taaggaggtt aaagataaag gattaatcga
     2461 taaatatatg actaatgctg caaatgcgat gaatgatgac agtggtttta tttctaagta
     2521 tggtagtttt ttcttgaaag gaattaagat tacaatatta atttcactta tcggtgttgc
     2581 attaggttct attttaggtg cattcgttgc gttaatgaaa ttaagtaaaa taaaaattat
     2641 ttcatggatt gcttctatct atatcgaaat attaagagga acaccaatgt tagttcaagt
     2701 atttatcgta ttctttggta taactgctgc attaggacta gacatttcag ctcttgtttg
     2761 tggaacaatt gcattagtta ttaattcctc agcttacatt gctgaaatta ttcgtgcagg
     2821 tataaatgct gttgataaag gccaaatgga agccgcacgt agtctaggtt tgaattatag
     2881 acaaacgatg aaaagtgtaa ttatgccaca agcaattaaa aatattttac cagctttagg
     2941 taatgaattt gtcactttaa ttaaagaatc atctattgtg tcaacaattg gagttggcga
     3001 aattatgttt aatgcacaag tggttcaagg tatttcattt gacccattca caccattaat
     3061 agtggcagca gcattatact ttgtcttaac atttgtactt acacgcatta tgaacatgat
     3121 tgaagggaga ttgaatgcca gtgattaaaa taaacaatct taataaagtt tttggagata
     3181 atgaagtttt aaaagatatc aatcttgaaa tcaatcaagg ggaagtagta gcaataatag
     3241 gtccatctgg tagtggtaaa agtacattgt taagatgtat gaatttatta gaagtaccta
     3301 ctaaaggtca agtgattttt gaaggcaatg acttaacgga aaaagggaca caagtagata
     3361 aactacgtca aaaaatgggt atggtatttc aaaacttcaa cctatttcca cataaaaaag
     3421 ttgtcgataa tattatttta gctcctaaat tattaaagaa agataataac gatgaattac
     3481 ataaggaagc attgtcgtta ttagataaag tgggattaaa agaaaaagca gatgtatatc
     3541 cgaatcaatt atcaggtggt caaaaacaaa gggtagcaat tgcaagagct ttagcaatgc
     3601 atccagatgt tattttattc gatgaaccaa cttcagcatt agatcctgag gtagttggtg
     3661 atgtattaaa agtaatgaaa gacctagcca aagaaggtat gaccatggtg gttgtgacac
     3721 atgaaatggg atttgccaaa gatgtaagtg acaaagtcat atttatggca gatggcgttg
     3781 tcgtagagtc aggcacacca gtcgaaatat ttgaacaacc gcaacatgaa agaacacaaa
     3841 atttcttagc aagagtatta taacaaccta acgaagcttg aatatatgat acgcaccaca
     3901 aagttatatc atatattcaa gctttttatt attaaaatta tgtgaaatga aacctcaata
     3961 tgaataatgg ttgtgttaaa ataacatagt attattttat ggggggcata cgattggata
     4021 caaagcaatt aaagcaagat atcattgact atgcatatac aattggtatc gacagtattg
     4081 gatttactac tgccgatccc tttgatgaat tgaagcaaaa gctagaagca tatcatgcaa
     4141 atggttatgc ctcaggattt gaagaatctg atattgcttt acgaacggag cctaaattat
     4201 ccttaccaac agcaaggtca atcatagcaa ttgcagttgg ttatcctaac aaactgaaag
     4261 gtgcacctaa gagtgttaga ggtgatcgca gaggcttatt tgctagagca tcgtggggtc
     4321 aagattatca tacaattatg cgtaaacgat tagacatgtt agctgcattt attgaatcta
     4381 aagttccaga tgttgaaatc aaatctatgg tagatacggg tgtattatca gatagggcag
     4441 tagcagaacg tgcaggttta ggatttgttg gtcgtaatgg ctttgtcatc aatcctaaac
     4501 taggaacatg gacatacctc ggtgaaatgt tagtcagtat accttttgaa cctgatgatc
     4561 cattattaga tagctgtggt gattgtacaa tttgtgttga tcgttgtcca acaagtgcat
     4621 tagttggaaa tggccaatta aatagtcaga aatgcattag ttttttaacg caaacaaaag
     4681 gctatatgcc tgaccaatat cgttataaaa ttgggaatag attatatggt tgcgatacgt
     4741 gtcaacaagt ttgtccgaaa aatagaggta ttaataccga acaagatgac atcattttgg
     4801 aaccagaaat tttaaagcca agattagtac ctttactacg tatgtctaat aaagaattca
     4861 aacaaacata tggtcacctt gcaggtgctt ggcgtggtaa aaagccgata caacgaaatg
     4921 ctattttagc attggcgcat tttaatgaag tggatgcaat tccagaattg aaaaaagttg
     4981 caacaacaga tgaaagaccg ttgattcgag caactgctta ttgggccatt ggtcaaattc
     5041 ttggggaaga agcaagagat tttatcaatg ctaattatga tcaagaggat gcagaagttc
     5101 agaatgaaat gataaaagga ttagacacaa ggagagaata gtaatatgac aaatcatatc
     5161 gttttatatc aaccagaaat cccagctaat actggtaaca ttgcacgtac gtgtgcaggt
     5221 acaaacacac atttacattt aattaaacct cttggattta gaactgatga taaaatgtta
     5281 aaaagagccg gtttagatta ttgggaattt gtgaatatta cgtatcatga tagtattgaa
     5341 gcattttttg aagtcacaaa tggcgagtat tacttattaa caaaatttgg taaaaagact
     5401 tatagtgatt ttgatttttc aaatcatgac aaagattttt acttcatttt tggaaaagaa
     5461 acgacaggat taccagactg ggtgaaagag aaatatcaag acacagcgtt aagaattcct
     5521 atgagtgaac atattcgttc attaaattta tcaaatactg ctgcgttgtt aatttatgaa
     5581 gcgttacgtc aacaagattt tccgggattg aattaaaatt tactcatttt cattataaaa
     5641 atgatgctta agtgctatat ttagagtata agcatgttaa agttaaatgg tttgatatat
     5701 tgaaagttag ggtataataa ttattaatat cgccgttttt atgtaaaatg caaactaaga
     5761 ggagtgtaga aatcatggca atgaacttta aagtctttga caatagtcaa cttgtagcag
     5821 aatatgctgc tgatattatt agaaagcaat ttaacaataa tcctactaca attgcaggtt
     5881 ttcatttaga tacagatcaa gcgccagttc tagatgaatt aaagaaaaat gttgaaaaac
     5941 atgctgttga ttttagccaa ataaatattt tagattatga cgataaaaaa tcatatttcg
     6001 aagcgttagg tgtaccagca ggtcaagttt atccaattgc ttatgaaaaa gatgcaatcg
     6061 aattaatcgc tgataagatt aaaactaaag aaaataaagg gaaattaaca ttacaagttg
     6121 tttctatcga tgagcaaggt aagttaaatg ttagtattcg tcaaggacta atggaagcaa
     6181 gagaaatttt cttagtagtg acaggtgcta ataaacgaga tgtagttgaa aaattatatc
     6241 aagaaaatgg taaaacaagc ttcgaaccag ccgatttaaa agcacataga atggtaaatg
     6301 ttattcttga taaagaagcg gctgcaggtt tacctgaaga tgttaaagct tactttacgt
     6361 cacgctttgc ttaattaaaa atctgtgagg tattgattat gtctaaagac aaagatccaa
     6421 aattaaatta tcatgaagaa gaaaacagta tggtaacgga ttttgaagat ttaaaagaat
     6481 taggtaaaga aatggaacaa atctctgatc aaaatgatca agaaaagaat tctgaagaag
     6541 acagtcagta aagtattata attgtgtaat attcagttat ttatcatgta aataatgtat
     6601 agtatagaac atatatcaaa taggattgat tatcttgaat atgaagttag gtagcatttt
     6661 gttacctagc ttttttatta tattcataga agctgacttt ctgctagttt cagtgttggg
     6721 gctgtttccc caacttacat tgtttgtaga aattgaggat ccaatttttc tatgttgtgg
     6781 ccccgccaac ttgcatgtta ttttaagctg actttccacc aacttctgag tttgtggccc
     6841 gtttccctaa tttgcaatgt ctgtagaaat tagaaatccg atctcactat tttggggcct
     6901 cgccaacttg catgttattt taagctgact ttccaccagt ttctgagttt gtggcccgtt
     6961 tccccaattt gcaatgtctg tagaaattgg gaatccaatt ccactatgtt ggagctccac
     7021 caacttgcat gaaatagaag ctgacattcc gaagcctcta tgttgtggac tgcagtcaac
     7081 tactgccaat ataactttgt aaagcataaa acattgattt gtgtaccaga catagtaata
     7141 tatcaaaagt tcaattttaa atattaaaac accatttaaa cggaattgat aattaattac
     7201 tccagacata attgaaagaa gtcatctatc agtaatattt ttaatgaaat cattaaaccg
     7261 cgtttcttaa tatgccaaga ttatgatatg ctattgaaga atgaaattag aggagattta
     7321 tatatttatg ttaggaaaat actggattca attattgata gcaacagtta ttataagttt
     7381 aatatcaatt aaagcttttc cattggcaat tggggcatta tatttacctg ttattttcaa
     7441 agtaataaaa ttacaactca atttatcaaa aggattaatt gatgatgtta atgcgcaaac
     7501 attcattaaa agtaatcaat caggtattgt aatcagtgta atttgttgtt tattaataac
     7561 agcaatattg tattatacac ttgatgggtt ttatgcgagt ttaacaggag ttttaggtac
     7621 gcttgttgct ctaaatccat atacgacaat tgttagtgct gtattatata tacttacagc
     7681 tattgctacg gttgaagcta ctaaaacaaa gtatcgtaat taaacttagt tcaataataa
     7741 ctaaataatg atagataaca cactaatcgt atgaccgtat cactgtaata aagggtgaac
     7801 acgtgcgatt agtgtatttt tatttcaaat tatgaaaacg ttttaatcat aaattgctca
     7861 attaaattat ttaatgatat actacagtta aataaacagt gataagggga gaaattgaat
     7921 gtcagtaaga attgaacatg atacttttgg agaaatagaa gtacctgcag ataaatattg
     7981 gggtgctcaa acagaaagaa gtaaacgtaa tttcccagtt ggtaaagagc gtatgccaat
     8041 cgaagtagtt tatggttttg cacaactaaa gcgtgcagca gcaatagcta attttgattt
     8101 aggaaaatta agcgaggcaa agaaagatgc cattgtatac gcatgtgatc aaattttatc
     8161 aggtgaatta gatgaacact tcccactagt tgtatggcaa acaggaagcg gtacacaaag
     8221 taatatgaat gtgaacgaag tagtaagtta tgttgctaat atgtatttaa aagatcatca
     8281 aagtgatgaa agtatccacc caaatgatga tgtaaataaa tctcaaagtt cgaatgatac
     8341 attcccaact gctatgcacg ttgcattata tcaagaggtt gaaacaaaat tagaacctgc
     8401 attaaaactt ttaagaaata ctttgaaaga aaaagaagat aaatttgatt caattattaa
     8461 aattggtcgt acacatttac aagatgcaac gccgatcaaa ctaggacaag agattagtgg
     8521 ctggcgttat atgcttgacc gttgtgaaac aatgttatct gaatctaaga agcacatttt
     8581 aaatcttgcc atcggtggta cggctgttgg tactggtatt aatgcgcatc ctgaatttgg
     8641 tgataaagtg gcacattata tttcagaaaa tacgggttat ccatttgtat cttctgaaaa
     8701 taaattccac gcacttacag cgcatgatga agttgttcaa ttgcatggaa cattgaaggc
     8761 attagcagga gacttaatga aaattgctaa tgatgtgaga tggttggctt cagggccacg
     8821 agctggtttg gcagaaattt ctatccctga aaatgaacca ggttcatcaa ttatgcctgg
     8881 taaagttaat cctacacaat gtgaaatgtt aacaatggtt gcagtccaag taatgggtaa
     8941 tgatacagtt gttggcttcg caagttcaca aggtaacttt gaattgaatg tttataaacc
     9001 agttattatg cataatacac tacaatcaat ttatctttta gctgatggta tggaaacatt
     9061 taataacaat tgtgcagtgg gcattgaacc aatcgaagag aatattgata attatttaaa
     9121 tcaatcatta atgttagtta ctgcattaaa tccacatatt ggttatgaaa aagcagctca
     9181 aattgctaag aaagcccata aagaaggttt aactttaaaa gaatctgcaa ttcaaactgg
     9241 atatgttaca gaagaacaat ttgaagcatg gattaaacca gaagatatgg tagatcctca
     9301 ttaattaaat attaatataa aataaagttt gggcattaca ggtagatgta gtgttcaagc
     9361 tttatttttt gtctatgtca aattgaactt ataagtctga tttgatatag agccatgaaa
     9421 aggaaaaact tttcatggag tccgttttat tatagaacct attttttata tgatgaattg
     9481 ttgtacatgt attggccaaa caacctgtga taaaaatctt atgatgtcat gtattgtcca
     9541 tagatacacg taacgcagtt gaaaaagact acaagtcatt atccaaggat gttataaaat
     9601 gaataattac taaaatatca aaatataaaa tcctaagcat cgtaaaaaac aaaaaaaccg
     9661 aacatctgaa aaaattaata attttccatc tatccgattt aagttgatac ttgatttagt
     9721 tacaatgcgg tattgcatgt ttaaatatca tgcaaaacta atcaaattag tcatccaaag
     9781 aaacggaaat gacttcctta gtcaagggat gcacaaactc tattttgtaa ctttcaagtt
     9841 ctaattgtct taacgttgaa tcaccatata aaggatcacc aataacaggg tgaccaattt
     9901 cagctaaatg cacacgaatt tgatgagtac gtccagtatc caacttgatt tcaagttggc
     9961 acacagcttc ttttatcatt tttgaagtta aaatgtgtgt aattgcacgc tgtccagtag
    10021 gagacacacg tcgtttattc gaatggaatt tatctttacc aattggcata tcaatcgttt
    10081 gtggttttaa aggtaaaagt gccttaacat ttgctttgta tatccgcgta atatcattgt
    10141 cttctaacat gcgatcaaga atttttttca ttaaaggatt tttagcaaca attaataatc
    10201 ctactgtttc ctggtccagt cgatgaattg gttcgacata gtcactatca atagtgtaaa
    10261 tcacatgatt cattaaagta ttgctttctt ttaaatcatt agggtgagtc ttaacacctt
    10321 taggtttcat tacgattgcc atatcatcgt cttcgtattt aatttgtgca taacgataac
    10381 ttggaacata attacttttt tcatcgatgg ttggaacaaa tacatcgtcg ccagtatgca
    10441 ctttatccat taatcgcgca ggtttatcat taatagtaat atctttagac atatttaaat
    10501 gatgtaaatc ttttttaggt accttaagtt gttggaaaat atctcgtaaa cttaagtcat
    10561 taaagttttc tggtatttta aatttcataa aatcctccta tagtctttaa tatcatatca
    10621 caaatcagtt ctccgaaata taactattga gtaatcaagg ccattttatc atgtttacgt
    10681 ctaagattta aaaatataat aactcaatgt ataatagtta atacacgtaa aaatgaattg
    10741 gagcgatttg atggaacaaa ggacgcgact agctttatta aaagagattg ccgaattttt
    10801 aaacgaagaa actgaaatgt atagcatgac ccaaggtgcg ttaaaatatt taattgaggg
    10861 cagtaatttc acgacaggct ggatattttt catcaatagc gtaggtgaac atgaacttgt
    10921 atcacatgtg gctctaccac aatctttgac agcagaccac tgtcactata ttaaagatgg
    10981 gtcttgttgg tgtgtgaaag catttaatca acgtaggcta atgaaagcgt cgaatatcgt
    11041 caattgttct cgtattaact tagcctctaa agcatttcct agccaaaatg acaatattac
    11101 acatcatgca acggtaccac ttaaatcggg tcaagaacaa ttcggcattt taaatgtagc
    11161 gtctccaaat actgaaatat atagcgatga agatttagag ttgctagagt ctgtcgcatt
    11221 tcaattaggg tcagccatca aacgtattta tttaacagac cgtgaaaaag aagcggcaaa
    11281 aattaatgaa cgtaatcgtt tagctagaga tttacatgat tcagtaaatc aaatgttatt
    11341 ttctgtaaaa ctaacagcac atgctgcgta tggcatgtca aacgaatcca tcgctaaaca
    11401 agcattcaag acgattgagg aaacgagtca aaatgccgtt aatgaaatgc gtgcattgat
    11461 ttggcaactt aagccagttg gacttgagca aggtttaatt catgctttga cagcatatag
    11521 taaattaatg catatacaat taaatgtaaa tgtagaaggt ttaatcgatt tatctaatga
    11581 aatcgaagaa aacatataca gagcattaca agagtgtatt aataatgtta agaaacatgc
    11641 tgatacgaat aaaatggatc ttacattaaa acaaatgaac gatatactat atattgacgt
    11701 gattgattat ggtcaaggat ttgagatcga taatgttcag atagcttcat cacacggtat
    11761 taataacata aaacaaagag ttaaattatt aagaggtaaa gtgacctttc attctcagcc
    11821 aacaaagggt acacaaatac aattcacaat tcctataaaa tagcttgatt taaggagata
    11881 acccatgaac aaagtaatat tagtagatga ccattatatt gtgcgacaag gattgcgatt
    11941 tttattatcc acgattgaaa acatagaagt tttacaagac tttgcagatg gagaaacatt
    12001 tttagaatat ttaaaagagc atgagcaccc tgatattgtg ctattagatt tagtgatgcc
    12061 tggcatgaat ggtattgaaa ttacggaata tattaaggca cattatccgg atattaaagt
    12121 tttggtatta acaagttatg ttgatgatga acatgtaatt tcagcaatca ataaaggtgc
    12181 tgatggttat gaaatgaaag acgttgagcc tcagcaatta attgaaacta ttagacgagt
    12241 tatgaacggt gaaaaaatga tacatcctaa ggcacaagat gtattcgaaa cagttagcca
    12301 aaaaccacac tacacgaata agttgtcaaa gagagaaatt gaagtgttac gtgaaatggt
    12361 taaaggtaaa acaaataaag agattgcaga aactttattt gtatctgaaa aaacaattaa
    12421 aacacatgtc agtcatatat ttagtaaatt acaagttagc gatcgtacac aagcagcaat
    12481 ttatgcaatg gaaaataagt tgatttagaa tgagctttta aatatttgtc gaaaaataga
    12541 caatttttaa tctgattttg atgaattttt tatagcgata gggtttatta tacagttttc
    12601 tttgtaaatt tgatatcatt ataaaagtgt aagttttatc aaatctagtg attatgcata
    12661 aaaatttact tcatttactt tcgcttcatt ataaagtaaa cttaactctt gttgacttga
    12721 tcaaaattta atacaaatgt gaaattgact cttttcattt tattaaaaat atattaagat
    12781 gtaatctgtg tttcaagaaa tgtgtattat tgcaatttct tgtgtccgga taaaattccc
    12841 caaacagaaa ggtagataac agaatggata gacagagttt tacagattta attcaaacaa
    12901 aatttaaaat ggttcgtata gaggctggtt atacgcaaga tactatggcg caaacaattg
    12961 gactttctaa aaagacttta gtacaaatcg aaaaagagag agtattacca aactggacaa
    13021 cttgtatttc aatttgtgca ttattcagag actctgaagt attaaacagt acatttggct
    13081 gtgatccatt agaaatcgtt caaacaattt caagaaatca ctgtgcatat ccaaaccatg
    13141 caccaacaag tgatatttat tggaataaca ttgagactcg taatgggtac attttacaaa
    13201 gtaacaaagt aagcaatatt tatcgtgtat taaatccaga taaccaacca attttcggta
    13261 cttctaaaat gagagaagct gaaacatact ttaacagaaa tgctaaagaa gaattagtac
    13321 atatctaggc ctatatactt ttaataaata ataaaagcga tgtaacacac ctgtttcgag
    13381 aggtgaggca tcgctttttg tatgtaaact aataataaat tgagcagaaa gtgaaatgat
    13441 taatgaatta aaaaataaaa atagtggtat aatgaataac tatgttgtca caaaggagtc
    13501 aaaattatga atgcactatt tattatcata tttatgattg tagtaggggc gataattggt
    13561 ggcattacta atgtaattgc aattagaatg ctgtttcacc cctttaaacc atactatata
    13621 tttaaattta gagtcccatt tacaccaggt ttaataccga aaagacgcga agaaattgca
    13681 actaaaattg gccaagtgat tgaagagcat ttgcttacag aaactttaat taatgaaaaa
    13741 ttgaaaagcg agcaatcaca gcaagcaata gaatctatga ttcaacagca gttacaaaag
    13801 ttgacgaaag atcaattgtc aataaaacaa attacttctc aaatcgatat tgatttagaa
    13861 caagtattac aaactaatgg aaatcaatat attgaatcac aattgaataa ttattataca
    13921 aagcatcaaa accaaacaat agcatcttta ttgccaaatc aacttgtaac atttttaaat
    13981 cagcatgtag ataacgcaac agacttatta tgtgatcgtg caaggaatta tttatcatct
    14041 gcaaaaggca cacaagatat taatgatatg ttggatactt ttttcaatga gaaaggtaag
    14101 ttaattggta tgttgcaaat gtttatgaca aaagagagta ttgcagatcg cattcaacaa
    14161 gaacttatac gtttaacatc tcatcctaaa gcaagaacaa ttgtgacatc gttaattacc
    14221 aatgaatatc aaacttttaa agataaacca ttgaatgaac tgttagatgc atcgcagttt
    14281 aatgaaatcg ctgaaaatct atcagtgtat gtaacaacat atgcaagcaa acaagcgaat
    14341 aagccagtgg ttacgttaat gcctcaattc gttgattatt tagaaggtca actctctagc
    14401 aaattagcaa atttaatcat tgaaaaatta tcgattcact tatctacaat tatgaaaaaa
    14461 gtggatttac gaggattaat tgaagaacaa attaatacgt ttgacttaga ttatattgaa
    14521 aaattaatta tagaaattgc taacaaagaa ctaaagttga ttatgtcatt aggtttcatt
    14581 ttaggtggta ttattggatt tttccaaggt ttggttgcaa tctttgtcta aaaaagcgag
    14641 atgtggtaaa gtaatatcga acgttttaat aaacatcaca acaaaggagt gctataacca
    14701 tggcagtaaa tttatatgat tatgcaaatc aattagaaca agctttaaga gaaagcgaag
    14761 aatacaaagc aatcaaagaa gcattcgcta atgtaaaagc taacgaagaa tctaaaaagt
    14821 tattcgacga gttccgtgaa actcaaatta acttccaaca aaaacaaatg caaggtgaag
    14881 aaattgctga agaagattta caaaaagcgc aagaacaagc gcaagcaatt gaaaaagatg
    14941 aaaacatctc tgcattaatg aatgctgaac aaaaaatgag tcaagtattc caagaaatca
    15001 accaaattat cgttaaacca ttagacgaaa tttacgctga ctaatattaa aataatattt
    15061 ttcaatcctt taaatagagg aatagactgc cgacagagtc cgagactttg tgggtagttt
    15121 ttttagtttt gataacggaa gttagaggct ctctgtcaaa ttgggcagat gcattcagta
    15181 acggaagtta ggctagtaaa cgtgtcctaa cttccttttt gcattttgaa gtgtaaagtt
    15241 ttcaacataa tactattagt tcggtcatgt atcggactga tggaaaagcg tttcactttt
    15301 aatgactcat taagaacggc ctgaaaatgt ttggcgtatt aagtgcaatg atagttttga
    15361 catttagttt ctaattggtc attactgccg agcaaatcta gtagagtaat catgtaaatc
    15421 tttaatgtgc catttgattc actagcggtg ttaataacta cggaaattgc atttccgact
    15481 gaaatttttg aaaaatatca acgtacgcta caaataaaat tttaaactgt tataaatgtg
    15541 tctcaatttc atatgttcat cgacgatatg aagcgtatta tggtaaaatg aagaaataat
    15601 aaacttgtta ataaataaaa catcacgatt tgactaaagc actttattat tgtgtagata
    15661 atagtttttt aacgaaataa aaatggcgac tggttttaat aaatcagcta atgaatcact
    15721 acacctataa gtatgaatat agtgattaga atgctttgta tagttggatt ttgcaaaatt
    15781 gatgttagaa cctttaatta aggagatatg gatgtatggt taaatttatt cattgttcgg
    15841 atttacattt agatagtcct ttcaaatcta agagtcatat aagccctaaa atttttgagg
    15901 atgttcaaaa aagtgcttat gaaagtttta aaaatattgt agatattgca ttacaacaag
    15961 atgttgattt tgtaattatc gcgggtgatt tatttgatag tgaaaacaga acattacgtg
    16021 ctgaaatttt cttaaaacag caatttgaac gtttacaaaa tgaacaaatc tttgtttatg
    16081 tttgtcatgg taatcacgat cctttatcat caaagatttc atcaaactgg ccagataatg
    16141 tttctgtatt ttcaaataaa gttgagacgt atgaagcaat tactaaatct ggtgaaacaa
    16201 tttatattca cggatttagt tatgaaaata gagcaagtta tgagaacaag attgatgaat
    16261 atccatcaag tcaaggccaa aaaggcatac atattggtgt cttgcatggt acgtatagta
    16321 aatcttcagt taacgaaaga tataccgagt tcattttaga agatttaaac agtaaattgt
    16381 atcattattg ggctttaggt catatacatg aacgtcaaca attaagtgat atgcctgtaa
    16441 ttaactattc aggtaatatt caaggtagac attttaatga gcaaggtgaa aaaggttgct
    16501 tattaatcga gggtgaccac ttaaaattaa agactaaatt ttatcctaca cagtatatta
    16561 gatttgaaga agcaactatt gaaacggata agacatctaa gcaaggttta tacgaggtca
    16621 ttcaaaactt taaagaacaa gtgagagaag aaggaaaagc cttttatcgt ttaacgcttg
    16681 ttattaatag tgagacatta atttcacctc aagatttatt acaagttgaa gaaatgatta
    16741 cagattatga agaaaacgaa aatcaatttg tatatattga tgagttaaaa atacaatatg
    16801 cacaaaatga tgagtcacct ttagttaatg aattttcagc ggaattatta gtcgatcaaa
    16861 ctgtttttga taaagcgatg tcagatttat atttaaatcc aagggcatct aagttcctag
    16921 acgattatgg aacattcgac catacagcat tagttaatcg tgctgaagaa atattaaaag
    16981 ctgaaatgag aggtgaacaa aatgataatt aaatcacttg aaatttatgg ttacggtcaa
    17041 tttgttcaac gtaaaattga atttaataaa aacttcactg aaatttttgg tgaaaatgaa
    17101 gcgggtaaat cgacgattca agcattcatc cattcgatat tatttggatt tccaactaaa
    17161 aagtctaaag agccaagact agaaccacgt ctaggtaacc aatacggtgg taaattagta
    17221 cttattcttg atgatggctt agagattgaa gttgaacgaa ttaaaggcag tgctcaaggt
    17281 gatgtgaaag tatatttacc taatggtgct gtgcgtgatg atgcttggtt acaaaagaaa
    17341 cttaattata tttctaaaaa gacatatcaa ggtatctttt catttgatgt actagggctt
    17401 caagacattc atagaaatct aaatgaaaaa caattgcaag attatttatt acaagcaggg
    17461 gctttaggat caactgaatt cacgtcaatg cgcgaagtga ttaatcgtaa aaaagatgaa
    17521 ttatataaaa aatcaggtaa aaatccgatc attaatcaac aaattgagca attaaaacaa
    17581 ctagaaagtc aaattcgtga agaagaagca aagctagaaa catatcatcg cttagtagat
    17641 gatcgagata aatcatcacg tcgattagag aatttaaagc ataatttaaa tcaattatca
    17701 aaaatgcatg aagaaaaaca aaaagaggtt gctttacatg atcattcaca agaatggaag
    17761 tctctagaac aacagttaaa tattgagcca atcacattcc cagaaaaagg tgtggatcgt
    17821 tacgaaaaag cacgagcgca taagcaatcg ttagaaagag atattggttt aagaaatgag
    17881 cgtttagctc aacttaaaga agaagcgact caattagagc cagttaaaca atctgatatt
    17941 gacgccttca ttagtttgaa tcaacaagaa aatgaaatta aaaataaaga atttgaactt
    18001 actgcaatcg aaaaggatat tgcgaataaa caacgtgata aagatgaatt gcaatcaaat
    18061 attggttggt ctgaaacgca tcatgacgta gatagttcag aggcaatgaa aagttatgtc
    18121 agtgagcaaa tcaagaataa acaagaacaa gctgcataca ttaaacaatt agaacgtagt
    18181 ttagaagaaa ataaaatcga agataatgcg gttcatagcg aactagattc tgttgaagaa
    18241 aaaatagttc ctgaagagac ttttgaaaag aaaaaagaat actcacaaca agtcattgaa
    18301 ttaaacgaaa aagagaactt gtatagcaaa ttgaaagaac gtttcgaaat tgagcaacaa
    18361 gaaaaacaaa aacgtcaaaa attgttacga acaacgttta ttcttttaac attagtaggt
    18421 attggtttaa ctgccttctc atttatttca aacaacatgt tgtttggtat tattttcgca
    18481 gtattaactt tagtatttgt tatcggtatc atcatgtcta aatcaaaaga agtagattat
    18541 agcgaggcca tcacagatga aatcgaagaa attaaagcac aactagccat tttagatgaa
    18601 aattatgact tagattttga tcttgatgag caatatcgca ttcgtgatca ctggcaacaa
    18661 gctttgaaaa ataaagatat tttagaagag aaacgtcagt atattgaagg tcgtttaaat
    18721 gatgcgaaag ggcgtcatga tgaattacaa tcgacagttg agaacgttaa ggatgaatta
    18781 tacttatcat ctaaaatttc aaatgattta attgttgata gtatttctac aatggcgaat
    18841 attaaagcat tagatcaaca tatcagtgat ttaaatcaac aacgccagca attggttcaa
    18901 gaattagata cattctacaa tcatgctgaa gctgttacaa aatcacaatt tgtttatttc
    18961 aacaaattat ctttattcca cgatgtgcaa caatggttga aatcagctga agacacgaat
    19021 gaaaaatggc gtattaatgc tgaaaatacc aaacttgtta caaatgaatt aaatcattta
    19081 aatgctcaat tagaagaaaa taataaagaa attacagcac tattcgattt tatcaatgtt
    19141 ggtactgaag aagattttta ccaacatcat gaagattacc aaacttatac tagtaatttg
    19201 agtcgtttta atgatttaac taaatatctt gagaaccaaa actattctta tgaattaagt
    19261 tctagtttaa gtgaaaaaac aactgcacaa cttgaagaag aagatcattt attggctact
    19321 caagttgacg aatataatga gcaatatctt gaaatgcaag cacaagtcag tgatttaagt
    19381 gcacaaatca atcacatgga aactgataca acgcttgcta atttaagaca tgaatatcac
    19441 agtcttaaaa atcaacttaa tgatatcgca aaagattggg caagtttaag ttatttacaa
    19501 agtttagttg atgaacacat taaacaaatt aaagataaac gtttgcctca agttattaat
    19561 gaagcggtag aaatattgaa gcatttaaca gatggcagat atacgatgat taactataat
    19621 gaagattcaa ttacggttaa acatgttaat ggtcaattat atgatcctgt tgaactaagt
    19681 caatctacaa aagaattact ttatgtagct ttacgtatca gtttaattaa ggtactaaga
    19741 ccatattatc cgttcccatt aattgttgat gatgcatttg ttcattttga taaaaaacgt
    19801 actgaaaaaa tgttgaatta tttacgatca ttatcagaac actatcaagt actatacttt
    19861 acatgtgtaa aagataatat tgttccatca aaagaagtga ttacattaaa caaaatagag
    19921 gaaggcggga aacgatgaga aatatagaga atctaaatcc cggagattca gttgatcact
    19981 ttttcttagt gcataaagct acacagggtg taacagcaca aggtaaagat tatatgacat
    20041 tacatttgca agataaaagt ggtgaaattg aagcgaaatt ttggacggct acaaaaaatg
    20101 atatggcaac aatcaagcct gaagaaattg tacatgttaa aggtgacatc ataaactatc
    20161 gcggaaataa acagatgaaa gtcaaccaaa ttagactagc gacaactgaa gatcaattaa
    20221 aaacagaaca atttgtagat ggtgcacctt tatcaccggc agaaatacaa gaagagattt
    20281 ctcattattt gctagatatt gaaaatgcta atttacaacg tatcacacgt catttattga
    20341 aaaaatatca agaacgattt tacacatatc cagctgctag ttctcatcat cataactttg
    20401 cgagtggctt aagctatcat gtattaacga tgttacgtat tgcaaaatca atttgtgaca
    20461 tttatccatt gttaaacaaa agtttgttat atagtggtat tattttgcat gatattggta
    20521 aagttagaga attgagtggt cctgttgcga cgtcgtatac agtcgaaggt aacttattag
    20581 gacacatctc gattgcgagt gatgaagtag ttgaagcagc tcgtgaattg aacattgaag
    20641 gagaagaaat catgttgtta agacatatga ttttatctca tcatggtaag ttagagtatg
    20701 gttctccaaa actgccatac ttaaaagaag cagaaatttt atgctatatc gataatatcg
    20761 atgctagaat gaatatgttt gaaaaggcat ataaaaaaac tgacaagggt cagtttacag
    20821 ataaaatatt tggtcttgaa aatcgtagat tctacaatcc tgaatcactc gattaagaat
    20881 taaaagatat cggacagatg agtatactga aacatgagct catctgtccg atttttaatg
    20941 cgaaaaaacc gagcggccgt ggtatgattt ttgaaccacg gtcgctcggt tttgtgttat
    21001 tggctcatgc cggattgtcc gccttgtgcg ccaccttgtt taagtttttc agggtttaag
    21061 attttatctt cgacaactga tttaatatca cgatctttaa agtcaacatc gtattctttt
    21121 aatagatctt tgtatgcatc agtcaataat tttggatttt tttgtacttt ctgatcgact
    21181 aatttttctt tcaggctttg tttttcactg ttaaagtctg ttggtttatc agctttaata
    21241 atatgatatc caaagcttga tttaacaacc tctgatactt caccatcttt aagcttaaat
    21301 agtgcttttt caaaatcttt atcagtttgt cctttaagaa cataacctaa ttcgccatct
    21361 tttttagctg aaccagtatc cattgattct tttttagcga tttcaccaaa tttacttgga
    21421 tcttttgaaa cttctttttg aatttcttca gctttttgtt tcgcttcttt atcatctaag
    21481 ccttctttgt cgcttttctt agatttaact ttaattaaaa tgtgtgaagc tttcttgctg
    21541 tcttctttaa tttcagaatc agagatttta attttatctg atagtaattc tttatgataa
    21601 gcagcagtac gtaaattttc tttatattta tcggctgtta aaccttgctg ttgaagggcc
    21661 ttttcaaatt tatctttacc gccgtattgc ttttgcattt tttcaatttg ttcgtcaatc
    21721 ttcttatcat taactttatt tttatattta tcagctaaaa ttttatttaa catttcagta
    21781 aatgatgcat ttgcaatttg atctttaccg atttttttca ttgtatctgc aactgttacg
    21841 tctccagctt tagaagaaat taatgtattt tctttagagt ctgtggcact agcgccacaa
    21901 gcgcctaata ataaagcact agctgttacc ggaacgatta atttgtttat catcttcata
    21961 gttgaaactc ctttgtaagt taatcatcgt gaatataaca tagttttata ttatcaccaa
    22021 tcaataaccc atgacatcag actagagcat taggtttagg ggtagaaatt gatgaaaata
    22081 aaaaaacaag gtctatatac atttgattgt aagctattac atagcctaaa aacaaataat
    22141 aacaccttgc tataattatt ttgttataaa tgatatggat ttttaatttt tttcaaaata
    22201 ttaatacctg tactatgttc ttctatatat tgtccataat tagaaaatat aattattttt
    22261 aaatataata aatctgtaat ggaaataccg aaattcaatg ccaataagaa cataaaataa
    22321 tgtccatatt gcgggaatga aattgttaga taaacgaaca ttgcagataa gataattaac
    22381 ggcaatatta agttaatgca aaaataaaat ttgtgtacag gttgattgac gtaggtatta
    22441 tagaatataa gccattttct tttagttaac ttatgaactt taaacgattt tctatatggt
    22501 aagaaaaat
//
LOCUS       DALAAR010000044        22495 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000044 DALAAR010000000
VERSION     DALAAR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.046"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            227..1138
                     /gene="rapZ"
                     /locus_tag="O2458_001731"
     CDS             227..1138
                     /gene="rapZ"
                     /locus_tag="O2458_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCZ3887596.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1135..2130
                     /locus_tag="O2458_001732"
     CDS             1135..2130
                     /locus_tag="O2458_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCZ3887597.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2240..3184
                     /gene="whiA"
                     /locus_tag="O2458_001733"
     CDS             2240..3184
                     /gene="whiA"
                     /locus_tag="O2458_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCZ3887598.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3758..4345
                     /gene="clpP"
                     /locus_tag="O2458_001734"
     CDS             3758..4345
                     /gene="clpP"
                     /locus_tag="O2458_001734"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCZ3887599.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4588..5490)
                     /locus_tag="O2458_001735"
     CDS             complement(4588..5490)
                     /locus_tag="O2458_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCZ3887600.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6192..6821
                     /locus_tag="O2458_001736"
     CDS             6192..6821
                     /locus_tag="O2458_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCZ3887601.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            7524..8537
                     /locus_tag="O2458_001737"
     CDS             7524..8537
                     /locus_tag="O2458_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCZ3887602.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            8590..9600
                     /gene="gap"
                     /locus_tag="O2458_001738"
     CDS             8590..9600
                     /gene="gap"
                     /locus_tag="O2458_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ3887603.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            9739..10929
                     /locus_tag="O2458_001739"
     CDS             9739..10929
                     /locus_tag="O2458_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCZ3887604.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            11051..11812
                     /locus_tag="O2458_001740"
     CDS             11051..11812
                     /locus_tag="O2458_001740"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCZ3887605.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            11815..13332
                     /locus_tag="O2458_001741"
     CDS             11815..13332
                     /locus_tag="O2458_001741"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCZ3887606.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            13462..14766
                     /gene="eno"
                     /locus_tag="O2458_001742"
     CDS             13462..14766
                     /gene="eno"
                     /locus_tag="O2458_001742"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCZ3887607.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            15106..15564
                     /locus_tag="O2458_001743"
     CDS             15106..15564
                     /locus_tag="O2458_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887608.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            15631..15864
                     /gene="secG"
                     /locus_tag="O2458_001744"
     CDS             15631..15864
                     /gene="secG"
                     /locus_tag="O2458_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCZ3887609.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            15980..16720
                     /locus_tag="O2458_001745"
     CDS             15980..16720
                     /locus_tag="O2458_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ3887610.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            16754..19126
                     /gene="rnr"
                     /locus_tag="O2458_001746"
     CDS             16754..19126
                     /gene="rnr"
                     /locus_tag="O2458_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCZ3887611.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            19148..19612
                     /gene="smpB"
                     /locus_tag="O2458_001747"
     CDS             19148..19612
                     /gene="smpB"
                     /locus_tag="O2458_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCZ3887612.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            19703..20064
                     /gene="ssrA"
                     /locus_tag="O2458_001748"
     tmRNA           19703..20064
                     /gene="ssrA"
                     /locus_tag="O2458_001748"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            20386..20667
                     /locus_tag="O2458_001749"
     CDS             20386..20667
                     /locus_tag="O2458_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887613.1"
                     /translation="MATEKELFIFVTSLSGCSYKEMSRVLIFSLESICLLTQSAIVDE
                     TEIKEVVVKKCISQHTIVAIFIVLDDKLFLEFLVFIICNYTTSFTNINV"
     gene            complement(20934..21257)
                     /locus_tag="O2458_001750"
     CDS             complement(20934..21257)
                     /locus_tag="O2458_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887614.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(21273..21526)
                     /locus_tag="O2458_001751"
                     /pseudo
     CDS             complement(21273..21526)
                     /locus_tag="O2458_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21611..22339)
                     /locus_tag="O2458_001752"
     CDS             complement(21611..22339)
                     /locus_tag="O2458_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCZ3887615.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
BASE COUNT         7913 a         3297 c         4203 g         7082 t
ORIGIN      
        1 ttgaaaaaag cttgttgcaa gcgcattttc attcagtcaa ctactagcaa tataatatta
       61 tagaccctag gacattgatt tatgtcccaa gctcctttta aatgatgtat atttttagaa
      121 atttaatcta gacatagttg gaaataaata taaaacatcg ttgcttaatt ttgtcataga
      181 acatttaaat taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga
      241 aaaagaaaaa agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc
      301 tttggttatt caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt
      361 gttattgcct aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc
      421 aattgcaatt gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa
      481 agtcaaaagt gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa
      541 attaatttca agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag
      601 atcgttaatc aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc
      661 taattttgtt atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg
      721 atactatgaa gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa
      781 acatgggatt cagatggatg cagatttagt atttgatgta cgatttttac caaatccata
      841 ttatgtagta gatttaagac ctttaacagg attagataaa gacgtttata attatgttat
      901 gaaatggaaa gagacggaga ttttctttga aaaattaact gatttgttag attttatgat
      961 acccgggtat aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg
     1021 acaacatcga tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga
     1081 atataatgtt tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga
     1141 caaataaaag ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta
     1201 agagaattcc caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca
     1261 gggaaaatca gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca
     1321 gctttaagtg attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat
     1381 caaattagcg gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat
     1441 gatttcggac atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt
     1501 ccatctacaa atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt
     1561 ggagaaacaa atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac
     1621 gatgtgcaac caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt
     1681 ggaccagggt cattatatac gagcgttatt tctaacttat gtgtgaatgg tatttcagat
     1741 gcgttaattc attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg
     1801 gaaacagatg gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa
     1861 ccgtttattg attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa
     1921 tatgaagaaa aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc
     1981 ataaatgtaa aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat
     2041 aatactaaag tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt
     2101 cctttcgtac caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata
     2161 atagagctgt gaaaaaaatg aaaatagaca gtggttctaa ggtgaatcat gttttaaata
     2221 agaaaggaat gactgtacga tgagctttgc atcagaaatg aaaaatgaat taactagaat
     2281 agacgtcgat gaaatgaatg caaaagcaga gctcagtgca ctgattcgaa tgaatggtgc
     2341 acttagtctt tcaaatcaac aatttgttat aaatgttcaa acggaaaatg caacaacggc
     2401 aagacgtatt tattcgttga ttaaacgtgt ctttaatgtg gaagttgaaa tattagtccg
     2461 taaaaaaatg aaacttaaaa aaaataatat ttatatttgt cgtacaaaga tgaaagcgaa
     2521 agaaattctt gatgaattag gaattttaaa agacggcatt tttacgcatg aaattgatca
     2581 ttcaatgatt caagatgacg aaatgagacg cagttacttg agaggagctt ttctggcagg
     2641 tggctcagtg aataaccctg aaacatcttc gtaccatttg gaaatttttt ctcaaaatga
     2701 gagtcatgca gaaggcttaa cgaaactaat gaatagttat gagttgaatg ccaaacattt
     2761 agagcgaaaa aaaggaagta ttacgtattt aaaagaagcg gaaaagattt cggattttct
     2821 tagtttgata ggtggctatc aagcgttatt aaaatttgaa gacgtacgta ttgtaagaga
     2881 tatgcgtaat tctgttaacc gactcgttaa ttgtgaaacg gccaatctaa ataaaacagt
     2941 tagtgctgcg atgaaacaag ttgagagcat taaattgatt gataaagaaa ttggtattga
     3001 aaatttacca gacaggttga gagagattgc tagaattcga gtagaacatc aagaaatttc
     3061 gttgaaagag cttggagaaa tggtatcaac tggtccaatt tcaaaatcag gtgtaaatca
     3121 ccgattaaga aaactgaatg atttagccga taagattaga aatggtgaac aaatagaatt
     3181 ataagtaaga aggtgttttt ggtagtgaat tatcaaaaac acttttttta tgattaaaaa
     3241 gtgtttaatg aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa
     3301 attcaataac caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg
     3361 tcataagaaa atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca
     3421 aaaacaaaat cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta
     3481 ttgtatttta ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa
     3541 tcttacgtgt tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg
     3601 agaagtaaaa taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag
     3661 cgtcaaacaa tgtaactatt taaagtcaaa gtgtttgacc aaatttgact taatatgtaa
     3721 aataatgagt aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat
     3781 tgaaacaaca aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg
     3841 tattattatg ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt
     3901 attattctta caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg
     3961 tggaagtgta acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt
     4021 tcaaacaatt tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc
     4081 aaaaggtaaa cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg
     4141 tgctcaagga caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga
     4201 aaaattaaac cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga
     4261 cacagatcgt gataacttct taactgcaga agaagctaaa gaatatggct taattgatga
     4321 agtgatggta cctgaaacaa aataattcaa agtaaagagt agactaagct gtctgctctt
     4381 tttgtatgag taaaccaagg tgtcaataat ttgtttacta tactttgagc ggaaatatga
     4441 ttgaatgaag ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa
     4501 aggaacattt caatcctttg tttgtaagtc gctctagaca ttacatttag tacatatgtt
     4561 gtttctaatg ctcattaatg gtattgatta ttctttaatt aaatcttcaa gtgccatttt
     4621 taaattacta tatttaaatt ggaatcccaa tgcttgaatt ttattaggta atactttttg
     4681 agtatccaat actactgttg acatttgacc aagtatgaga cgcattgcaa gacttggtgc
     4741 ccaagtttca tgaggcttat gcatagctct tgctaaagtg tagccaaata aattttgacg
     4801 ttcaggtata ggtgcagtta aattaaacgg accactagct gactcgttat ttattaaaaa
     4861 taaaatagct tgaattaaat cattgatatg aatccatgaa taccattgtt gaccagaacc
     4921 taatttacca ccaatgtaat attcgtatgg tagtttcatt gtttgtaacg caccgccttc
     4981 attcgataaa attataccga aacgaccgat gacaactcgt gtacctaatt gttcaaattg
     5041 ttgtgcgaaa cgttcccatt gatacacaat atctgataag aaatcaaaag gtaaagtttt
     5101 ataaacttct gtgtaactca taaataaatc aggaggatag taaccagtgg cactagcatt
     5161 aaataaagct ttaggtgctt tattacgtga tttaaacaat tcatataaag cttgcgtaga
     5221 ttgaattcta cttagcatta gcgtttgttt atattccggt gtccatcgtt tattcaatgt
     5281 agcacctgct aagttgatga ccacatcgat attttgagga actttgtgtt cccacccaga
     5341 tttagcccag ttgacatatg aaattttctt atcatttgaa atttggtcgt gtcgcgttaa
     5401 tatcgtgata tgtgaatctg attttttaat ttcattaact aattgagatc caaccatacc
     5461 agtcccacca gtaattaagt attgtttcat tatcattcac cccatgtaat tttgtattta
     5521 gttactattt aataatcatt attgttgttc aaaggttata cattatttag acaataatat
     5581 gtcaataact ttttttgaat tgtattttaa taaaaatgat ataagttata ataaaggtgt
     5641 accttcgata acgaataaac atctcttaaa agtatgtgta aaacgctgca tgatacaaac
     5701 gaaggtaaaa atttgactcc ctttagtagt ggacccgtac gttaatcgtg cgggtcgttt
     5761 ttatttattt cttattccca ttatacatca atttaaagca ttaatttttt aaacaaattt
     5821 aagaatacat agtaatataa caatctaaac ataaaaactt ttaacacaac acttaaacca
     5881 atgctttaat tttcaatacg tagctataat ttgttgtaaa atcaaaaagg ttaaaatgtt
     5941 aattttcaaa aaaggctcaa aatatgtttg atttagttat taaatgttaa gatatataag
     6001 actactattt ctttgtaaaa atgaatccga tttacgagtg agtaatagtg aaggcagttt
     6061 taagttgaag aaggcaaaaa gagtaaatgt ttattaatat ttgtagaaac taggtaagca
     6121 aattagttgt gaaaatgtta atggttgcgt gataatttct atatttaaat tagtttgaag
     6181 tgagggagag tatgtcgaat caaaattacg actacaataa aaatgaagat ggaagtaaga
     6241 agaaaatgag tacaacagcg aaagtagtta gcattgcgac ggtattgcta ttactcggag
     6301 gattagtatt tgcaattttt gcatatgtag atcattcgaa taaagctaaa gaacgtatgt
     6361 tgaacgaaca aaagcaggaa caaaaagaaa agcgtcaaaa agaaaatgca gaaaaagaga
     6421 gaaagaaaaa gcaacaagag gaaaaagagc agaatgagct agattcacaa gcaaaccaat
     6481 atcagcaatt gccacagcag aatcaatatc aatatgtgcc acctcagcaa caagcaccta
     6541 caaagcaacg tcctgctaaa gaagagaatg atgataaagc atcaaaggat gagtcgaaag
     6601 ataaggatga caaagcatct caagataaat cagatgataa tcagaagaaa actgatgata
     6661 ataaacaacc agctcagcct aaaccacagc cgcaacaacc aacaccaaag ccaaataata
     6721 atcaacaaaa caatcaatca aatcagcaag caaaaccaca agcaccacaa caaaatagcc
     6781 aatcaacaac aaataaacaa aataatgcta atgataagta gtatttagtc aaacaaaaat
     6841 gaaccagtat gacagacaac acaattaatt aggttgtctc gaatattggt tcttattttt
     6901 ataattgtta attaggggag agatgatact taaatagtta gttatttatt ttacggatag
     6961 tgaaatttat tttgagtgag gtgggacaga aatgatattt tcgcaaaatt tatttcgtcg
     7021 tcccacccca acttccattg cctgtagaaa ttggaaatcc aatttctcta tgttggggcc
     7081 ccaccccaac ttgcattgcc tgtagaaatt gggaggagcc aatttcacta tgttggggcc
     7141 cctgacttta attgaaaaaa gcttgttaca agtgcatttt cgttcggtta actactacta
     7201 atgtgacttt ttggattcta gagcattgat ttatgtccta gtctcaaata ataagcaaat
     7261 gattatcgag tcagtataag ggtatacatt tgactacagc gaataaaata aaacgttttg
     7321 ttgaataaca aatcgaaaat atattgcaag cgctttatca aattatttag aaaatttaag
     7381 ttttatgctt gcaatttttg aaatagaata gtactattgc aagtgtaaag aggttaattt
     7441 ttgtcccacg cgggacttaa aaaggcaacc actggttgtg acatatcctt atttacattt
     7501 ataaatataa ggaggaggta gtagtgaaag acttattgca agcacagcaa aagcttatac
     7561 cggatctcat agataaaatg tataaacgtt tttctattct tactactatc tcaaaaaatc
     7621 agcctgtcgg acgtcgaagt ttaagcgaac atatggatat gactgaacgt gtactgcgtt
     7681 ctgaaacaga tatgcttaag aaacaagatt tgataaaagt taagcctacc ggaatggaaa
     7741 ttacagctga aggtgagcaa ctgatttcgc aattgaaagg ttactttgat atctatgcag
     7801 atgataatcg tctgtcagaa ggtattaaga ataaatttca aattaaggaa gttcatgttg
     7861 ttcctggtga tgctgataat agtcaatctg ttaaaacaga attaggtaga caagcaggtc
     7921 aattacttga aggcatatta caagaagacg cgatagttgc tgtaactggc ggatccacga
     7981 tggcatgtgt tagtgaagca attcatttat taccatataa tgtattcttc gtaccagcca
     8041 gaggtggact aggcgaaaat gttgtctttc aggcaaacac aattgcagcc agtatggcac
     8101 aacaagctgg cggttattat acgacgatgt atgtacctga taatgtcagt gaaacaacat
     8161 ataatacatt gttgttagag ccatcagtca taaacacttt agacaaaatt aaacaagcaa
     8221 acgttatatt acacggcatt ggtgatgcgc tgaagatggc gcatcgacgt caatcacctg
     8281 aaaaggtcat tgaacaactt caacatcatc aagctgtcgg agaggcattt ggttattatt
     8341 ttgatacaca aggtcaaatt gtccataagg ttaaaacaat tggacttcaa ttagaagacc
     8401 ttgaatcaaa agactttatt tttgcagttg caggaggcaa atcgaaaggt gaagcaatta
     8461 aagcatactt gacgattgca cccaagaata cagtgttaat cactgatgaa gccgcagcaa
     8521 agataatact tgaataagag ataaaaagtt taatactttt taaatatcat tttaaaggag
     8581 gccattataa tggcagtaaa agtagcaatt aatggttttg gtagaattgg tcgtttagca
     8641 ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag cagtaaacga cttaacagat
     8701 gacgacatgt tagcgcattt attaaaatat gacactatgc aaggtcgttt cacaggtgaa
     8761 gtagaggtag ttgatggtgg tttccgcgta aatggtaaag aagttaaatc attcagtgaa
     8821 ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg atgtagtatt agaatgtact
     8881 ggtttctaca ctgataaaga taaagcacaa gctcatattg aagcaggcgc taaaaaagta
     8941 ttaatctcag caccagctac tggtgactta aaaacaatcg tattcaacac taaccaccaa
     9001 gagttagacg gttctgaaac agttgtttca ggtgcttcat gtactacaaa ctcattagca
     9061 ccagttgcta aagttttaaa cgatgacttt ggtttagttg aaggtttaat gactacaatt
     9121 cacgcttaca caggtgatca aaatacacaa gacgcacctc acagaaaagg tgacaaacgt
     9181 cgtgctcgtg cagcggcaga aaacatcatc cctaactcaa caggtgctgc taaagctatc
     9241 ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg gtgcacaacg tgttcctgta
     9301 gctacaggtt cattaactga attaacagta gtattagaaa aacaagacgt aacagttgaa
     9361 caagttaacg aagctatgaa aaatgcttca aacgaatcat tcggttacac tgaagacgaa
     9421 atcgtttctt cagacgttgt aggtatgact tacggttcat tattcgacgc tacacaaact
     9481 cgtgtaatgt cagttggcga ccgtcaatta gttaaagttg cagcttggta tgataacgaa
     9541 atgtcatata ctgcacaatt agttcgtaca ttagcatact tagctgaact ttctaaataa
     9601 ttttagtata gtttttattc aaatacgcta gtgctcagaa ctatttagca ttaattaaag
     9661 cttatgagta agcggggagc acaaacgctt ctccgcttat ttttatataa aatttcctaa
     9721 ttacaaggag gaaacaccat ggctaaaaaa attgtttctg atttagatct taaaggtaaa
     9781 acagtcctag tacgtgctga ttttaacgta cctttaaaag acggtgaaat tactaatgac
     9841 aaccgtatcg ttcaagcttt acctacaatt caatacatca tcgaacaagg tggtaaaatc
     9901 gtactatttt cacatttagg taaagtgaaa gaagaaagtg ataaagcaaa attaacttta
     9961 cgtccagttg ctgaagactt atctaagaaa ttagataaag aagttgtttt cgtaccagaa
    10021 acacgcggcg aaaaacttga agctgctatt aaagacctta aagaaggcga cgtattatta
    10081 gttgaaaata cacgttatga agatttagac ggtaaaaaag aatctaaaaa tgatccagaa
    10141 ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa atgatgcttt tggtactgcg
    10201 catcgtgagc atgcatctaa tgttggtatt tctacacatt tagaaactgc agctggattc
    10261 ttaatggata aagaaattaa gtttattggc ggcgtagtta acgatccaca taaaccagtt
    10321 gttgctattt taggtggagc aaaagtatct gacaaaatta atgtcatcaa aaacttagtt
    10381 aacatagctg ataaaattat catcggcgga ggtatggctt atactttctt aaaagcgcaa
    10441 ggtaaagaaa ttggtatttc attattagaa gaagataaaa tcgacttcgc aaaagattta
    10501 ttagaaaaac atggtgataa aattgtatta ccagtagaca ctaaagttgc taaagaattt
    10561 tctaatgatg ccaaaatcac tgtagtacca tctgattcaa ttccagcaga ccaagaaggt
    10621 atggatattg gaccaaacac tgtaaaatta tttgcagatg aattagaagg tgcgcacact
    10681 gttgtatgga atggacctat gggtgtattc gagttcagta actttgcaca aggtacaatt
    10741 ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta cgattatcgg tggcggtgat
    10801 tcagctgcag cagcaatctc tttaggtttt gaaaatgact tcactcatat ttcaactggt
    10861 ggcggcgcgt cattagagta cctagaaggt aaagaattgc ctggtatcaa agcaatcaat
    10921 aataaataat aaagtgatag tttaaagtga tgtggcatgt ttgtttaaca ttgttacggg
    10981 aaaacagtca caagatgaca tcgtgtttca tcacttttca aaaatattta caaaacaagg
    11041 agtgtcttta atgagaacac caattatagc tggtaactgg aaaatgaaca aaacagtaca
    11101 agaagcaaaa gacttcgtca atgcattacc aacattacca gattcaaaag aagtagaatc
    11161 agtaatttgt gcaccagcaa ttcaattaga tgcattaact actgcagtta aagaaggaaa
    11221 agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc gaagataatg gtgcgttcac
    11281 aggtgaaacg tctccagttg cattagcaga tttaggcgtt aaatacgttg ttatcggtca
    11341 ttctgaacgt cgtgaattat tccacgaaac agatgaagaa attaacaaaa aagcgcacgc
    11401 tattttcaaa catggaatga ctccaattat atgtgttggt gaaacagacg aagagcgtga
    11461 aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag aaagctgttg caggtttatc
    11521 tgaagatcaa cttaaatcag ttgtaattgc ttatgaacca atctgggcaa tcggaactgg
    11581 taaatcatca acatctgaag atgcaaatga aatgtgtgca tttgtacgtc aaactattgc
    11641 tgacttatca agcaaagaag tatcagaagc aactcgtatt caatatggtg gtagtgttaa
    11701 acctaacaac attaaagaat acatggcaca aactgatatt gatggggcat tagtaggtgg
    11761 cgcatcactt aaagttgaag atttcgtaca attgttagaa ggtgcaaaat aatcatggct
    11821 aagaaaccaa ctgcgttaat tattttagat ggttttgcga accgcgaaag cgaacatggt
    11881 aatgcggtaa aattagcaaa caagcctaat tttgatcgtt attacaacaa atatccaacg
    11941 actcaaatcg aagcgagtgg cttagatgtt ggactacctg aaggacaaat gggtaactca
    12001 gaagttggtc atatgaatat cggtgcagga cgtatcgttt atcaaagttt aactcgaatc
    12061 aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg ttttaaataa tgcaattgca
    12121 cacgtgaatt cacatgattc agcgttacac atctttggtt tattgtctga cggtggtgta
    12181 cacagtcatt acaaacattt atttgctttg ttagaacttg ctaaaaaaca aggtgttgaa
    12241 aaagtttacg tacacgcatt tttagatggc cgtgacgtag atcaaaaatc cgctttgaaa
    12301 tacatcgaag agactgaagc taaattcaat gaattaggca ttggtcaatt tgcatctgtg
    12361 tctggtcgtt attatgcaat ggatcgtgac aaacgttggg aacgtgaaga aaaagcttac
    12421 aatgctattc gtaattttga tgccccaact tatgcaactg ccaaagaagg tgtagaagca
    12481 agctataatg agggcttaac tgacgaattc gtagtaccat tcatcgttga gaatcaaaat
    12541 gacggtgtta atgatggaga tgcagtgatc ttctataatt tccgacctga tagagcagcg
    12601 caattatcgg aaatttttgc gaacagagca ttcgaaggct ttaaagttga acaagttaaa
    12661 gacttattct atgcaacatt cactaagtat aatgacaata tcgatgcggc tatcgtcttc
    12721 gaaaaagttg atttaaataa tacaattggt gaaattgcac aaaataacaa tttaactcaa
    12781 ttacgtattg cagaaactga aaaataccct cacgttactt actttatgag tggtggacgt
    12841 aacgaggaat ttaaaggtga acgccgtcgt ttaattgatt cacctaaagt tgcaacgtat
    12901 gacttgaaac cagaaatgag tgcttatgaa gttaaagatg cattattaga agagttaaat
    12961 aaaggtgact tggacttaat tattttaaac tttgctaacc ctgatatggt tggacatagt
    13021 ggtatgcttg agccgacaat caaagcaatc gaagcggttg atgaatgttt aggagaagtg
    13081 gttgataaga ttttagacat ggacggttat gcaattatta ctgctgacca tggtaactct
    13141 gatcaagtat tgacggatga tgatcaacca atgactacgc atacaacgaa cccagtacca
    13201 gtgattgtaa caaaagaagg cgttacactt agagaaactg gtcgcttagg tgacttagca
    13261 cctacattat tagatttatt aaatgtagaa caacctgaag atatgacagg tgaatcttta
    13321 attaaacact aatattgtaa aagatgttaa gtaaacgctt aatgacactt attttttgaa
    13381 aataatagta atatcatttt gttaaatgaa agaataaagc tataataatt atagaataac
    13441 tatttaaagg agattataaa catgccaatt attacagatg tttacgctcg cgaagtctta
    13501 gactctcgtg gtaacccaac tgttgaagta gaagtattaa ctgaaagtgg cgcatttggt
    13561 cgtgcattag taccatcagg tgcttcaact ggtgaacacg aagctgttga attacgtgat
    13621 ggagacaaat cacgttattt aggtaaaggt gttactaaag cagttgaaaa cgttaatgaa
    13681 atcatcgcac cagaaattat tgaaggtgaa ttttcagtat tagatcaagt atctattgat
    13741 aaaatgatga tcgcattaga cggtactcca aacaaaggta aattaggtgc aaatgctatt
    13801 ttaggtgtat ctatcgcagt agcacgtgca gcagctgact tattaggtca accactttac
    13861 aaatatttag gtggatttaa tggtaagcag ttaccagtac caatgatgaa catcgttaat
    13921 ggtggttctc actcagatgc tccaattgca ttccaagaat tcatgatttt acctgtaggt
    13981 gctacaacgt tcaaagaatc attacgttgg ggtactgaaa ttttccacaa cttaaaatca
    14041 attttaagca aacgtggttt agaaactgca gtaggtgacg aaggtggttt cgctcctaaa
    14101 tttgaaggta ctgaagatgc tgttgaaaca attatccaag caatcgaagc agctggttac
    14161 aaaccaggtg aagaagtatt cttaggattt gactgtgcat catcagaatt ctatgaaaat
    14221 ggtgtatatg actacagtaa gttcgaaggc gaacacggtg caaaacgtac agctgcagaa
    14281 caagttgact acttagaaca attagtagac aaatatccta tcattacaat tgaagacggt
    14341 atggacgaaa acgactggga tggttggaaa caacttacag aacgtatcgg tgaccgtgta
    14401 caattagtag gtgacgattt attcgtaaca aacactgaaa ttttagcaaa aggtattgaa
    14461 aacggaattg gtaactcaat cttaattaaa gttaaccaaa tcggtacatt aactgaaaca
    14521 tttgatgcaa tcgaaatggc tcaaaaagct ggttacacag cagtagtttc tcaccgttca
    14581 ggtgaaacag aagatacaac aattgctgat attgctgttg ctacaaacgc tggtcaaatt
    14641 aaaactggtt cattatcacg tactgaccgt attgctaaat acaatcaatt attacgtatc
    14701 gaagatgaat tatttgaaac tgctaaatat gacggtatca aatcattcta taacttagat
    14761 aaataatttt ctttataatc aaatgctgac ataattttag ttgaggatta ttatgacggt
    14821 ataaattaaa taaagatttt gagttcacgc ttaaataagt tcacgcttaa atttatagcc
    14881 tgccacagag ttgagactgt ggtaggtttt ttattttgaa gtattaatca taacagacta
    14941 ataatcatga ggtaactaat aacacatatt taacttgtat tcttaaactg gtataataaa
    15001 tttatgttga aatgaatatt gtatgacagg gtattcactt ttattaaaag gtaaaattaa
    15061 ataaaggttt tatagaacgt atttaaatat atgaggagta aacaaatggc tgatagaacg
    15121 aataaagaaa ttaaaacagg acgctttatt gcaactgcat caatcgtatt ctcaatatta
    15181 ttgattattc attactttgt ttcgttggat aatgcgactg ccaaagcatt acttaattta
    15241 acgaatcaaa acacttcaga taaagcgatt gattacattt taaacagctt tagattcact
    15301 ggtattatgt atattttggc ttatctagca ggcttcatca ctttttggaa tcgacatact
    15361 tatgtgtggt ggtttatgtt tgcagtttat gtatcaaata gtttgtttac gttgattaat
    15421 ttatcaatca caattcaagc aataaaagct gcacacggtg cgtacttaac attgccaatt
    15481 ttaattgtta ttataggttc ggttgcatta gcgatttata tgcttgttgt ttctatcaaa
    15541 cgtaaaagta catttaatcg ctagaaaatt gattttaaca ataaaaatat gatatactac
    15601 ttgtcgtata taaggaacgg aggacaattt atgcatacat ttttaatcgt attattaatc
    15661 attgattgta ttgcattaat aactgttgta ctactccaag aaggtaaaag cagtggactt
    15721 tcaggtgcca tcagtggtgg tgctgagcag ttattcggta aacaaaaaca acgtggcgtc
    15781 gatttattct taaatagatt aacaattatt ttatcaatat tattttttgt acttatgatt
    15841 tgcataagtt atcttggtat gtaaggtccg gcgatgtaaa tgtcgggctt ttttatttat
    15901 aattaagaat gtaatagttt aacaataagc tatgtaaaat atatagccta gttaagtatg
    15961 caaagggagc gttagattta tgcagataaa attaccaaaa cctttctttt ttgaggaagg
    16021 taaacgtgcc gtgttattac tacatggttt tacaggcaat tcgtctgatg ttcgtcaatt
    16081 aggtcgattt ttacaaaaga aaggttatac atcatatgca ccgcaatatg aaggccacgc
    16141 ggcaccacca gatgaaatac tgaaatctag tcctttcgtt tggtttaaag atgcgttaga
    16201 tggttatgat tatcttgttg aacaaggtta tgatgaaatt gttgttgctg gtctatcatt
    16261 aggtggggat tttgctttaa aattaagctt aaatagagat gtaaagggta ttgtaacgat
    16321 gtgtgctcct atgggtggca aaactgaagg tgccatttat gaaggctttt tagaatatgc
    16381 acgcaatttt aaaaagtatg aaggtaaaga tcaagagact attgataatg aaatggatca
    16441 ttttaaacca actgaaactt taaaagaact aagtgaagca ttagatacga ttaaagagca
    16501 agttgatgaa gtgttggatc ctattttagt gattcaagca gaaaacgaca atatgattga
    16561 tccacaatcc gcaaattata tatatgacca tgtagattct gatgacaaaa atatcaagtg
    16621 gtacagtgaa tctggacatg ttattacgat tgataaagag aaagaacaag tatttgaaga
    16681 tatttatcaa tttttagagt cattagactg gtcagaataa aaagagattt taacattaga
    16741 aaggaggggc ataatgaatt taaagcaatc tatagaagag attattaatc aacctgaata
    16801 tgaacctatg tcagtgtcag attttcaaga tgcattaggt ttaagcagtg ccgactcgtt
    16861 tagagattta attaaggtgc ttgtggagtt agaacaatca ggattaatcg aacgtacaaa
    16921 aacagacaga taccaaaaaa agcatagtta tagaggtcaa tcaaaattga taaaaggaac
    16981 gttaagtcaa aataaaaaag gctttgcatt cttaagacct gaagatgagg atatggaaga
    17041 tatatttatt cccccgacga aaattaatcg tgccttggat ggagatactg ttattgtaga
    17101 aatccatcaa tcaaaaggtg aacataaagg taaaatcgaa ggggaagtta agtcgattga
    17161 gaagcattct gtaactcaag ttgttggtac gtatagtgaa gctagacatt ttggctttgt
    17221 tattccggat gataaacgta ttatgcaaga tattttcatt cctaaaggtc aaagtttagg
    17281 cgcagtcgat ggtcataagg tacttgtaca aattactaag tatgctgatg gttcagataa
    17341 tccagaagga catatttctg ctattttagg acataaaaat gatcctggcg tagatatttt
    17401 atctattatc tatcaacatg gcatagaaat tgaatttcct gatgaagtgt tacaagaagc
    17461 tgaagcagta cctgatcata ttgaaaatac tgaaattaaa ggccgtcatg atttacgtga
    17521 tgaattgaca atcacaattg atggtgctga tgctaaagac ttagatgacg caattagtgt
    17581 taaaaagtta gcgaacggta atacgcaatt aactgtaagt attgctgatg tcagctatta
    17641 tgtaacagaa ggttctgcat tggataaaga ggcatatgat agagcgacaa gtgtatatct
    17701 tgttgaccgt gtaattccaa tgattccaca tcgattaagt aatggtattt gttcattgaa
    17761 tcctaatgtt gatcgtttaa ctctaagctg tcgcatggaa atcgatgcta gtggtcgcgt
    17821 tgttaaacat gaaatttttg atagtgttat acattctgat tatcgaatga cgtatgatgc
    17881 ggtaaatcag attattactg aaaaggatcc taacattcgc gaacaatata atgaaattac
    17941 gcctatgcta gatttagcac aagatttatc taatcgtttg attcaaatga gaaaacgacg
    18001 tggtgaaatc gattttgata ttagtgaagc aaaagtatta gttaacgaag acggtatacc
    18061 aacagatgtt caattaagac aacgtggcga gggtgaacgt ctaattgaat catttatgtt
    18121 aattgcaaat gaaacagttg ctgaacattt tagtaagtta gatgtacctt ttatttaccg
    18181 agtgcatgag caacctaaat cagatcgctt aagacaattc tttgatttta ttacaaactt
    18241 tggcatcatg attaagggta ctggcgaaga tattcatcca acaacacttc aaaaggttca
    18301 agaagaagta gaaggtcgac ctgaacaaat ggtcatttca acaatgatgt tgcgttcaat
    18361 gcaacaagcg cattatgatg atgtgaactt gggacatttt ggcttatcag ctgaatatta
    18421 tacgcatttt acatcaccaa ttagacgtta tcctgattta acagttcatc gtttaatccg
    18481 taagtattta attgagaaat caatggataa caaagaagtg aagcgttggg aagacaaatt
    18541 gcctgagtta gctgaacata cttctaaacg tgaacgtcgt gctattgagg cagaacgtga
    18601 tactgatgaa ttgaaaaaag cagaatatat gattcaacat attggtgatg aatttgaagg
    18661 tattgtcagc tcagtagcta acttcggtat gttcattgaa ttgccaaata cgatagaagg
    18721 tatggttcat attgcgaata tgactgatga ttattaccgt tttgaagagc gtcaaatggc
    18781 attaattggt gagcgtcaag ctaaagtatt tagaattggt gacacagtta aggttaaagt
    18841 gacgcatgtt gatgtagatg aacgattaat tgattttcaa attgtaggta tgcctttacc
    18901 gaaaaatgat cgatcacagc gcccagcgcg aggtaagaca attcaagcca aaacgcgtgg
    18961 taaatcatta gataaatcaa aatctgatga taagggtcgt aagaaaaaag gtaagcaacg
    19021 taaaggtaaa aaccaacgta ataatgataa atcaggtaat agtaagcata agccatttta
    19081 taaagataaa agtgtgaaaa agaaagcacg tcgtaagaaa aaataagcag caatgaggtg
    19141 agtatgaatg gctaagaaga aatcaccagg tacattagcg gaaaatcgta aggcaagaca
    19201 tgattataat attgaagata cgattgaagc gggaattgta ttgcaaggca cagaaataaa
    19261 atcaattcgc cgaggtagtg ctaaccttaa agatagttat gcgcaagtta aaaacggtga
    19321 aatgtatttg aataatatgc atatagcacc atacgaagaa gggaatcgtt ttaatcacga
    19381 tcctcttcgt tctcgaaaat tattattgca caagcgtgaa atcattaaat tgggtgatca
    19441 aacacgtgag attggttatt cgattgtgcc gttaaagctt tatttgaagc atggacattg
    19501 taaagtatta cttggtgttg cacgaggtaa gaaaaaatat gataaacgtc aagctttgaa
    19561 agaaaaagca gtcaaacgag atgttgcgcg cgatatgaaa gcccgttatt aagcgattta
    19621 gttgcttaat cgggctatat ttgatatagt tatatgtgct tttgtaaatt acaaaagtat
    19681 gatttgtttg atttattatt tcggggacgt tcatggattc gacaggggtc ccccgagctc
    19741 attaagcgtg tcggagggtt gtcttcgtca tcaacacaca cagtttataa taactggcaa
    19801 atcaaacaat aatttcgcag tagctgccta atcgcactct gcatcgccta acagcatttc
    19861 ctatgtgctg ttaacgcgat tcaaccttaa taggatatgc taaacactgc cgtttgaagt
    19921 ctgtttagaa gaaacttaat caaactagca tcatgttggt tgtttatcac ttttcatgat
    19981 gcgaaaccta tcgataaact acacacgtag aaagatgtgt atcaggacct ttggacgcgg
    20041 gttcaaatcc cgccgtctcc atatttgtag cctacagcct ttgtggttgt gggctttttt
    20101 attttgtgtt tttcagggga taatgcattg cagaatttgt tgtgagtatt gatatagcag
    20161 tgtttgtata ggtgtttatt tgatggagga aagagtaata agtgattatg aattagtttt
    20221 tgagatataa ggggacagtg atgtgtgtca aataagtgtc aaaaaagttg gattctgagt
    20281 tttacattca acattgttca tgaagaaact tctttatacg caaaaaattc tccatgttat
    20341 atatgtcaat ataaaaatgt gaatcgtcta cacttaattg gataaatggc tactgaaaaa
    20401 gaacttttca tttttgttac gtcactaagt gggtgtagtt ataaagagat gagccgagtt
    20461 ttgatatttt cattagaatc aatatgccta ttaacacaat cagcaatagt tgacgagacg
    20521 gaaataaaag aagtcgtagt taagaaatgc atttcacaac ataccattgt agccattttt
    20581 attgttttgg atgataaact ctttttggaa tttttagttt ttataatttg caactacact
    20641 acttctttta ctaatattaa tgtctaagta atcgataaaa aattttccat tgaataaatg
    20701 agaagttaaa aactttactt aacctttctc attgcatttt cctattcacg attttaagaa
    20761 cccaacatac tacaaacgaa ttttaaaagg cgagagtaaa gcttacttgt ttattataca
    20821 tatttaaaat ccaagagtca gaacagacta ctcctcttta taactataaa aaatagctat
    20881 gaaaaaatct atcgtcatag attccttcat agctaatctt agtatgttta tttttatttt
    20941 aggatgctat ttatcaactc aacatataac tcactatttt tataaccttc taatatatca
    21001 ttaacttgtc taataggtat ttctggtact tctctaatgt tttccaattt tgttttaaat
    21061 tgtttttttg ttatttgctc tttatttgta gccaattgga acaagtaaga atctagcata
    21121 ttaatttctt tatatgaata catatatctt aataacacta aatctctagt ttttaagtta
    21181 ggcgctagtt cttcttgtaa ttgttctatt gattgtttca ttaataacaa tctcatttct
    21241 aattcttcat tattcatttt atcacactct ttttatatta atgcttgacc aacttgggaa
    21301 acccaaaacc ctatgcttct tgcagtagaa tctttaatac cagttcccat caatgcttgt
    21361 gaaacttgac cttgtacatt tccccatgta gcctcttctt gttttaatgc attattcaat
    21421 gcgggattta caaatttatc ccatcttttt tttatgattt tccggcacgg ggactgattt
    21481 ctttaacacc attaaacaca gattttttat ttttaatcat agctttatag tatcatgttg
    21541 gctaagctat aaataagtca gtttctctaa aaattaaata actgaatgta agacaatcaa
    21601 caaaccaaat ttatacttca tctaaaccac tgtggtcgtc atctttttgc ttttcttttt
    21661 ctttctctcg ttcttgttct tttttgtact cttcttcaaa ttctttttct ttcttttcta
    21721 cttcttctct tgtttccgct ctatgagaaa aatcttcggt tttaagttta ctaaatttga
    21781 atgatttaga atcaactgtt ttatcttctg agtatttatg gacatttaaa ttaatatttc
    21841 catcacctct taactcatag ataaacatgg cttgtgcagt tttgcctttt ttaatttgat
    21901 cttggttatg ttctgtccaa tctttatatt ttttatcact taaaagataa ccatctctta
    21961 atttatttac tgtattttta tcatcttgag tgatattaat atagtcatga gaaatagaag
    22021 atggatttaa atctttatcg tcttttttag cagtaatttc cattttaaaa gcgatatatt
    22081 tctttttctc atctttttca ttgatgataa acggttcttt tattttagct tcaaatttgt
    22141 cactaacaat agtatcgcct ttaattttta tatccatatt ttttttgctt ttaaattctt
    22201 taagttcttc atttaattct tcattgtcat tttctttctt tttgtgacta gtgctctctt
    22261 tttttgcact atcttgatga tgtccacaag cacctaagat aagtgtactt gctaataata
    22321 tccccattac ttttttcatt taacatgtct cctttatttc gcaaaaattt attttaaaaa
    22381 ctctaaatga cttatcattt tgagtaatta aacaaagttg atattttgtg agattctaag
    22441 atgatattaa ataattcttg taataatgat cctatgtatt gttgcaataa attaa
//
LOCUS       DALAAR010000045        22165 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000045 DALAAR010000000
VERSION     DALAAR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.047"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(136..801)
                     /locus_tag="O2458_001753"
     CDS             complement(136..801)
                     /locus_tag="O2458_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887616.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(801..1856)
                     /locus_tag="O2458_001754"
     CDS             complement(801..1856)
                     /locus_tag="O2458_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887617.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1992..2666
                     /gene="hssR"
                     /locus_tag="O2458_001755"
     CDS             1992..2666
                     /gene="hssR"
                     /locus_tag="O2458_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCZ3887618.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2659..4032
                     /gene="hssS"
                     /locus_tag="O2458_001756"
     CDS             2659..4032
                     /gene="hssS"
                     /locus_tag="O2458_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCZ3887619.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4195..4638
                     /locus_tag="O2458_001757"
     CDS             4195..4638
                     /locus_tag="O2458_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3887620.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4635..5090
                     /locus_tag="O2458_001758"
     CDS             4635..5090
                     /locus_tag="O2458_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCZ3887621.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5156..6634)
                     /gene="mqo"
                     /locus_tag="O2458_001759"
     CDS             complement(5156..6634)
                     /gene="mqo"
                     /locus_tag="O2458_001759"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ3887622.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7015..8613)
                     /locus_tag="O2458_001760"
     CDS             complement(7015..8613)
                     /locus_tag="O2458_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ3887623.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8930..10603
                     /locus_tag="O2458_001761"
     CDS             8930..10603
                     /locus_tag="O2458_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ3887624.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10756..11385)
                     /locus_tag="O2458_001762"
     CDS             complement(10756..11385)
                     /locus_tag="O2458_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887625.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11699..12214)
                     /locus_tag="O2458_001763"
     CDS             complement(11699..12214)
                     /locus_tag="O2458_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887626.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            12405..13409
                     /locus_tag="O2458_001764"
     CDS             12405..13409
                     /locus_tag="O2458_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ3887627.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(13455..13856)
                     /locus_tag="O2458_001765"
     CDS             complement(13455..13856)
                     /locus_tag="O2458_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887628.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(13892..14926)
                     /locus_tag="O2458_001766"
     CDS             complement(13892..14926)
                     /locus_tag="O2458_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3887629.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(14937..15053)
                     /locus_tag="O2458_001767"
     CDS             complement(14937..15053)
                     /locus_tag="O2458_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887630.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(15158..15589)
                     /locus_tag="O2458_001768"
     CDS             complement(15158..15589)
                     /locus_tag="O2458_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCZ3887631.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(15796..17073)
                     /locus_tag="O2458_001769"
     CDS             complement(15796..17073)
                     /locus_tag="O2458_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ3887632.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            17234..17857
                     /locus_tag="O2458_001770"
     CDS             17234..17857
                     /locus_tag="O2458_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3887633.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            17903..18847
                     /locus_tag="O2458_001771"
     CDS             17903..18847
                     /locus_tag="O2458_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCZ3887634.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(18933..20375)
                     /locus_tag="O2458_001772"
     CDS             complement(18933..20375)
                     /locus_tag="O2458_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCZ3887635.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            20558..20941
                     /locus_tag="O2458_001773"
     CDS             20558..20941
                     /locus_tag="O2458_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCZ3887636.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         7448 a         3890 c         3200 g         7627 t
ORIGIN      
        1 tagaaatcag cttttttaca ttgcctaaga acttaatgtc ccagcctgtt ttttgtaaac
       61 ccaaatcgta ttctgttatc taacttttat actactatgt cttaaatcat taacatttag
      121 cgtctttaca agccactaat cagttatttt gccatcttct aattcaatca ctcgatctgc
      181 atattcaaat aatcttcgat cgtgtgtaat cataatacca atcatttgtt gttctttaat
      241 ttgttgacgt atcatctcaa caacttttgt tgctctatcg gcatctaaac ttgctgtggg
      301 ctcatctgct aaaatgattt tcggattatt cataaatgct ctcataatcg caacacgttg
      361 cttttcaccg ccagataact gatgcggata tacattcaag cgatcttcta aaccaatatt
      421 tttcaaaagt tgtattgctc ttgttgaact ttgttgtttg gtcattcccg cttcttgacc
      481 tacgagtgtc aattgctcta tcacttttaa ataaggaact aaatgtgaag attgaaaaat
      541 aaaaccaata tcttccaatc gtaaatcact aggacgatgc tgtttatcaa acaatggcgc
      601 atcattgtaa agcactgtac cactcgtttg acttaacaat ccgcctaata tcgttagcaa
      661 tgttgttttc ccagaaccag aggcaccatt taaaatgaca aattcccctt gttccacttc
      721 aaaattaata ccttttaaaa cttttgtttc agacaaacct tctccgaaat ttttgacgat
      781 atcttcaacg actaatgcca ttattctgca cctccaattg cttcgatagg atccacttta
      841 aataatttga taaatgatag tgaggcacct aaaatcgcta ctaatataaa tatccccacc
      901 attaataaaa tattttgcgt tgttaagtaa aaaggcatcg ttaccggcat cataaatgat
      961 agtcctacta tgatgatcac agcaatacct accccaatta ttgttagtgt taaaatttgt
     1021 aaaactaacg cactcaataa atgtcttgtc ttaataccaa ttgctttcaa aatgccaatt
     1081 tgtgatattt tttgaatcgt cataacatag aaaaatgcac ttagaacgat tgctgtaata
     1141 gcaaacaaac taacaatcat catatttaac ggtgcttgct ctgcttgata actcgcaata
     1201 ttacctgtta aatctttttc actcacaact tgaacgtcat ttattttatt aagctcatca
     1261 cgttgttgtt gtgttaaatt tttcactgga taaaatgccg tattaacctt attcaatgca
     1321 ttaaatccgt tatcgttcaa tagcacaatg gaactatgcg catacattgt gtcgtttaaa
     1381 ataccagaaa ctctataact agttgaatta tttttaaaat gtaatctatc cccgactcta
     1441 atgttgtctg cagctaactt atcattaata gcgacctcat gagcattttt aggataatca
     1501 ccttttttaa gttgtatgcg ttgttgttta acgtcattca ttgtaatcac atcttgttct
     1561 ttattagata atttcaatgt ttgaatattc attttatatg gttcttgatg aattaccttt
     1621 ttaatttgat tctgttgcgt atcactaagt tgcgatttct caatttgcgg ctctttcatt
     1681 ttttgaacaa catattcatc attatcaaag tgttcaaata acgaaatatt ctccctacca
     1741 agcccttgag ctaaaccact aataaataac accataatac ttaataaaag aatgattaat
     1801 gtgattaaaa tataacgaaa tttgtaaaac ataatctctt ttatcgctaa tttcatatcg
     1861 attcacttct ccctatttct tctttagcgt cagtataagg ttgaaatatg aactcaatat
     1921 gaacaattgt ttttttacgc aaaaatattt tacatgtata ataaatgaaa aagataaggg
     1981 agtttatagc tatggtgcaa tgtcttgttg tcgatgacga tcctcgaatc ctaaattata
     2041 tagctagcca tttacaaaca gagcacattg atgcatacac acaaccaagt ggtgaagctg
     2101 cattaaaact tttagaaaaa cagcgtgtcg atattgcagt ggtagatatt atgatggatg
     2161 gtatggacgg ctttcaatta tgtaatacat taaaaaatga ttatgatata ccagttatta
     2221 tgttaacagc gcgggatgca cttagtgaca aagagcgtgc gtttataagc ggtactgacg
     2281 attatgtaac caaacccttt gaggttaagg aacttatttt tagaattcgt gctgtattac
     2341 gtcgatataa tatcaattca aattcagaaa tgactattgg caacttaacg ctaaaccaat
     2401 cctatttgga actccaagta tctaataaaa cgatgacgtt accgaacaag gaatttcaat
     2461 tattatttat gcttgcagca cgtcctaagc aaatctttac tcgtgaacaa ataatagaaa
     2521 aaatttgggg ctatgattat gaaggagatg agcgaacagt tgacgttcat attaagcgac
     2581 tacgccaaag attaaaaaaa ttaaatgcca cacttacaat tgaaacagta agaggacaag
     2641 gctataaggt ggagaatcat gtttaaaaca ctctatgcta gaattgcgat ttattccatt
     2701 acggttattt tatttagtgc attaataagt tttgtattaa caaatgttta ctatcattat
     2761 aatttaaaag catctaatga cgcgaaaatc atgaagacgc ttaaagaagc aagacaatat
     2821 gaacaatcag ctaaaccaac gcacattcaa caatatttca aacatttagg gcaaatgaat
     2881 taccaaataa tgaccattga tcaaaaaggt cataagacat tttatgggga gccatttaga
     2941 gaagatactt tatcacaaaa tgctattaac aacgtattaa ataatcaaga ttaccatggt
     3001 attaaagata aaccattcgc attattcgta actggatttt tcgacaatgt aacagataat
     3061 actgttggta ttaactttaa gacaaaagac ggttcaatag cagtatttat gcgtccagat
     3121 attggagaaa catttagcga gtttagaaca ttcttagccg tcttgttaat gttgttatta
     3181 tttatctcga tttcattagt tatcgcatca acctattcaa tcattcgtcc agtaaaaaag
     3241 ttaaagctag cgaccgaacg cttaattgat ggtgattttg aaacacctat caaacaaaca
     3301 cgcaaagatg aaattggaac attacagtat cactttaata agatgagaga gtcattggga
     3361 caagttgatc aaatgaggca gcattttgta caaaatgtat ctcatgaaat caaaacacca
     3421 ttaacacata ttcatcattt attaagcgag ttacaacaaa cttctgataa aacattaaga
     3481 caacaatata taaatgatat ctataccatt acaacgcagc tgagcggatt aacaactgaa
     3541 ttgttgctct tatccgaatt agataatcac caacatttat tattcgatga taagattcaa
     3601 gtcaatcaac tcattaaaga tatcattcga catgaacaat ttgctgctga tgaaaagtcg
     3661 ttaatcattt tagctgacct agaatcaatc aatttcctag gaaatcaacg tttgttacat
     3721 caagcattga gtaatttatt aataaatgct attaagtata cagatgttgg tggtgccatt
     3781 gacatcgctt tgcaacatag tcacaacaat atcattttca caataagtaa tgatggttca
     3841 ccaattagtc cacaagctga agcacgttta tttgaacgtt tttataaagt gagcaaacat
     3901 gataatagca atggtttagg tcttgcaatt acaaaatcca tcattgaatt gcatcatggt
     3961 acaatccaat tcacacaaag taacgagtat gtaaccacct ttactattac actgccaaat
     4021 aattcacttt aatctctacc tcctgaaaaa acatttctgt ttttatttca ggaggtatct
     4081 tttatattga ttagcgcttt ctagccactt aacatttaat ttctaccatt caacaaaatt
     4141 taaatatatc gatggtcatt tatggtttaa taattttgaa ggaggcatta acctatgatg
     4201 aaactcaatt tatttatcaa tgcaaatgag acggaatctt atattgatat acatgcacct
     4261 aaaatgaatg ataacgttca aagtattatt aatgcagtca atgatttaga caaatcacat
     4321 acattagtcg gatacataga caaagaaatc catatcatta atgtatcaga tgttataaca
     4381 ttccaagtta ttaataaaaa tgtaacagcc attacaagca atcaaaaatt caaactaaaa
     4441 ttacgacttt atgaactaga aaaacagtta ccacaacatt tcattcgcat ttccaaatca
     4501 gaaatcgtca acaagtatta cattgaaaaa ttattattag agccaaatgg acttatacgt
     4561 atgtatctta aagatgcaca ctacacctat tcttctagac gttatttaaa atctataaag
     4621 gagcgcttat caatatgaaa aatttgaaaa actcattatt tattagctta atcattggtt
     4681 tgtccctttc actgttcttt agtatgttat ttgcagacgg caaatattat ccactgaacc
     4741 cacaatcaac tattggcata ttgtattata cgcatttcac agaaactacc gtaatgttga
     4801 tttctatcat tttatggtta ctcataggcg tcgtcttttt ccttggagat tttatcttta
     4861 aatacacaga ttggagcatt actaaagcaa ctataatgca tttcataaca acgtatgtcg
     4921 gattcttgcc tttagcaatg cttgccggtt ggtttccact aacagtgcat tatctaatca
     4981 tattcacaat tatcttcatt gtggtttatg tattaatttg gataattcaa ttctttaaaa
     5041 acaagaacta cgtagacact attaacaagc aactgaaaca attaaaatga tttttattaa
     5101 ccactaaaaa acggtaatgt ctatcacatt aaaaaagaca ttaccgtttc gtttattatt
     5161 taacttgtaa atacttagtt acttcttcat taattttatg atataaatcc tcatgatctg
     5221 ttaagcgata accaaatgac ggcaccattt ctttaatctt tggttcccat cctttgaatt
     5281 catcacgata gcatctctgt aaaacatcaa acataatatc tacagctgtt gacgcaccag
     5341 gtgatgcacc aagtaatgca gctaatgtgc catcatctga cgtaataact tcagtaccaa
     5401 attgtaagtt acctttagaa tcctcagtat ccttgattac ttggacacgt tgccctgctg
     5461 taatcacttc ccaatcttca tttttagcat ttgggaaaaa gactcttaaa tcatccatac
     5521 gctcatcatt agataacatc aattgtgaca ctaaatactt cgtaagactc atttctttga
     5581 tacctgcaga taacatcgtc acgatattat ttggtttaac cgatttaatt aaatccatat
     5641 gtgaacctgt ttttaaaaat ttaggtgaga aacctgcaaa tggaccaaat aacaatgaac
     5701 gcttgccgtc tacaaaacgt gtatctaagt gcggcactga cattggtggc gcacccactg
     5761 ctgcttttcc gtacacttta gcatgatgac gatcaatcac ttcttgattt gtacagcgca
     5821 ggaataatcc acttaccggg aaaccaccaa tatgttttga ttgtttaatc ccagtctttt
     5881 ggagtaatgg taaactcgca ccgccagcac caataaatac aaaatcagat tcatatgttg
     5941 tcgtttcatt agtttctaaa tctttaactt taactagcca taccccagat ttctgtttct
     6001 tgatgtctaa tacattctgc ttatattgaa ttcccacacc acgttgctct aaattatcaa
     6061 ataactttgc agttaacgca ccaaagttaa catctgtacc agtttcatca taagttaaag
     6121 caattggttc atcagactta cgaccttcaa tcattaacgg tacccaagat ttcatttctt
     6181 cttcatcttg cgataatttc attttttcga ataaaatact tttctttaac gttgcaacac
     6241 gactttttat aaaagctaca ttatcttcgc caatgacaaa actcatgtgt ggcaccgctt
     6301 gaataaagcg atctgggtta tctaattgtc ctgttttaac taaatatgcc caaaactgtt
     6361 ttgaaatttg gtactgctca tttatcttaa ttgctttact acaatcaact gtgccatcct
     6421 taccttcttt tgtatagttc aactcgcaaa gtgctgaatg ccctgtaccg gcattattcc
     6481 atacatttga actctcttcg ccaggttgtg ctaatttttc aaacacttta atatttttct
     6541 caggtgataa ttctttaagt aatgttccta atgttgcact cataataccg ccaccaatta
     6601 agatgacatc tgttttgcta tgttgtgttg tcataacaaa tacagtcccc ctttttaaaa
     6661 tgaataatgg aacgttcgag aaatacgaca tattaatacc actttaaatc aataagtgtc
     6721 tcaataggtt cccatggtga tgctctatta ctatctacta tgtatattaa tattttataa
     6781 taacgaaact actttaatta tatactttat gtgcgatagc acaaagacaa aaccacaaac
     6841 cttaacagct atcaaccaat ttaatttgat tgctgattac tcactattca ttttaacata
     6901 aagcgctacc attattataa tgtcaaactt ctttcattaa tgatccataa aaaaaagatg
     6961 agatgttaga aaacatctca ccttctgtat atttatattt tgaaatacgt ttttttataa
     7021 taatgacaag atgaaagtcc aaatacagat gaatattagt aaacatacac tgtatttcaa
     7081 tgtcattttt aataattctg attccttacc aacttgtttt actgctgcag ttgcaattgc
     7141 aattgattgt ggtgaaatca attttgccgc tacaccacca actgtattag cagatacaag
     7201 aagtgaacca cttgttccaa tttgttgtgc aacagaagct tgaattggtg caaataatga
     7261 gttgttattt acaactgatc ctgtcataaa cacacctatc caacctaaaa ttggtgatag
     7321 aactgggaag acattacctg ctttagcaat accttgaccc attgctgcac ttaaaccacc
     7381 ataagttgtg attttagaaa ttgctaagat gaaacaaatt gtaagaactg gtaaccacaa
     7441 ctctttaaat gtaacgccga ataatctacc tgcatcttta aagttaacct ttttagacat
     7501 taaaattgta ataataatag ttaataaaat agctgtacct gtttgtccaa taatatttaa
     7561 agtcaatact aatggtttat gcgtaacttc gctgattgtt ccaggtaagt tgaatttaaa
     7621 tactaatgat gataaagcac catttggtaa gaataagttt ttaaagaatg gcgcactcca
     7681 aatcattaca atgactgtta atacaatgaa tgggctccat gcatgtaata ctgcttttgc
     7741 agaatgtgct tttgcaggtt caatttcttc atctttatta acacgataaa tgtgttttgg
     7801 ttggaatttt ttagaaaata ctgctaatgc taacattgtt aataacggtg gaataatatc
     7861 tgctaattca ggtccactga atacagttaa taatccttga gtaaatgtat aagtgattga
     7921 aactactaaa attgctggta atgtttcttt aacacctcta aaaccatcaa taataaagat
     7981 aagtaagaat ggaataatga aatttatgat tgccaatgtt aaagttgctg attgagaaac
     8041 acctaatact gaaacatctc caggtaattt caacgtttct acaacaccta cagggatacc
     8101 aatcgcacca aaagcaccag acgctgcatt tgcgactaaa cataacatcg cagcttttaa
     8161 tggattaaat cctaattgtg ttaataaaag tgcacaaatt gcaattggta caccaaatcc
     8221 tgctgcacct tctaaaaatg cgttgaatgc aaatccaata agtaaaactt gaatacgttg
     8281 gtcttgtgaa atatttgtaa tactatcttg aattgttaaa aattgtccag attcaacagt
     8341 aattttgtat aacaatactg ccatcataac gatatagcca atcggaatga taccttggaa
     8401 gaaaccttcg actactgcac cagaagcgat accaactggc aatttgaaaa atggtattgc
     8461 aattagtaat gttacaacaa gtgttgtaat agctgcataa atacctttca ttttaaaaac
     8521 agttaaacat agtaaaaaca gtacaatcgg tattgctgca attaaagacg ataataataa
     8581 attatcaaat gggttaaacg tatttactaa cataggctga ctcttctttc cataaagtag
     8641 tagtgtaatt gcttcattaa aatcatgctc tggctatagc attttgttat cgataacatt
     8701 ttacagaaca gtgagattaa tgtgattttt ttcacaatta aaatatacca ttttttcaga
     8761 ctattaacaa tatcactttc ctatttaatt gttttatttt tcattttaaa tctgttcatc
     8821 aaaatgaatt gtacttaatg taatgaagta acttatacta ttcatgaatt ttacataaga
     8881 ccgtgctaaa ataaggaaga tactacgatg aggaatggaa gttatagcaa tgattaaaca
     8941 gataaatgtt tctaatatgc aaaagtttga atctcaatta atgaaagctc agtctgaagg
     9001 ttatacacat gtcgttccat atgccaatga gataatgatt tatcaatcga tgttagatgc
     9061 ggttcaatta tatcctaagt ctatcgtggt tgattatact gtggatggac aatacaaaaa
     9121 tgattgtcac tatttcggtc aatcatcgat caacattgcc gattgggctc aaaacaataa
     9181 ttattatcct aacctgatat atgcgattca acagactctt gatttaatac attattactc
     9241 tgtagaaacg attttcgatt tagctttatt gacacttctg aaaggtgatt tatcaatcga
     9301 tggtcatgtc gtttttgatt ttaaagcccc tatagcaaca agtgcttcaa tatgggaaac
     9361 tattaaaact attgaagatt ttgatatgat gtctcagttt tatttgaata aaatggctta
     9421 tattgatcat catcctatac cttttcgcaa cctttttatc gaggattcag aacaacttaa
     9481 ttcgccagat aattggttgt attctaccaa gtttatgcta cctaaatggt tatataaaat
     9541 agcaaagcaa cgtgctgata acaaacaatt acaaaacttc ggcctgtata cgaagcaacc
     9601 aaacgtttta aaagatcaca tcgtttttat cggtgatcat catcaatata taggtaattc
     9661 aaaatattta tttacttact tcgttaaaca taatccaatg actgcctgtt attttgttac
     9721 tgacgatcgt cgcggaccac atttcatatc acctaagtct gaaaaagctg acgaattgat
     9781 taacagcgca cgcgtagtta tagttgaaaa tgatatacct gaaacgttac aaccaaacgg
     9841 tactttaatt caattacatc aaggcacacc aataatgcaa ttatttttag atagtaaaga
     9901 gcctattaaa aatatagaaa caccttttta tcgtgctaaa aggtataatc gctggttgca
     9961 attcgattat gttatacatt ctgctgatga tattagccac ttttatcaaa cggcttttcc
    10021 tagtcaccaa gcaaatgtat tggcttatgg taatcctaaa caccaatatt tactacaaaa
    10081 gagtaacgaa agtactactc aacaacagta taagaaatcg tttaaaataa atgatcaaaa
    10141 accagtttta ttatacgcac ctattggatt agtgagtgcg caacaattac cactatcaga
    10201 tgctttattc aaagcctatc atgttgtggt tcagggtgtt gatgaagcga tcttaccaga
    10261 agaggcgctt gttgcaccaa agtatcttag tgctcaagac ttaattctaa tgtctgatgt
    10321 cgttatcact gattactcaa atataatatt tgatgcgatg gctatagaca aaacagtagc
    10381 attatacact ccaaatcaca gtcaatacat tgagtctcaa ggtgttaacg aagatatttg
    10441 gcgtcatcta tcaaaaattt ggtatacaga ccgacaatta ttaattaata atttaatttc
    10501 ccaggcaata ccagtgatta aatatccaca aatacaacat aaagaacaac cattagaatc
    10561 tatttcacaa cttatattgt ctaagatgac atctaatcaa tagtgtttaa atttctcagt
    10621 ggctgtgaat gaggtttaaa agtactataa aacgtaaact ttgatacttt aaaatacgca
    10681 aaaaacggta aaccctaatt catattatag agtttaccgt tttatttttt aacttgcatc
    10741 atagttatat taacattatt gttggtagtt tggatcagta accattgctt gtccagtata
    10801 atcaaccgtt acaattgaat attttccatt tgcatttggg tctttaaaac taaacacata
    10861 cttatagttg ccattatgtt cttcaataga ataatcatta tacactttat tattactacc
    10921 aaatttattt gcttcattat tagccgcatt taaagctgtt tggaaatttg gcaattgctg
    10981 taaagcttga tttttatttc cattaaacgg ataaatttga cgtgcaaccg gcgcggcatt
    11041 ttgtccataa tatggtgcaa cgtaacttga tttttgatta ttattcgctt ggttattact
    11101 tgattggtta ttatttgttt ggttttggtc attgtttgtt gcatttgaat tagattgttg
    11161 ctggttatcg tttgcactat tatctttatt atctttgttt acgtctttac tatcatcttt
    11221 attatctttc ttatctttag atgaatcatt tgttttttta tcttgttgtt cagttttcgc
    11281 tttatcatct ttttctttat taccgtcttt ttgttggtca ctatcttgac cacatgcagc
    11341 taaaaataat gataatgcta gtaaccctgt aactaatctt ttcatacata tctcctccta
    11401 taattcgata ttcattgaat aatcttgaaa tacatatcta ccatgtgtat cttttcatgg
    11461 cttgttacca aagacgtcaa cattaatatg atctgaatta tcacaatatc cataactaca
    11521 atctaaggtt ctttttatta taccctaatt tttgttcatt attatttaat ttttgtgaat
    11581 tttatgtttt ctataaattt aattatttta ctttaacaat tcattacgca tttagcattt
    11641 caaggtatac acaatattta ttactatgat ttcattttat ctgctgcaaa aacaatcatt
    11701 ataactcttt ttccataatt aaatctgtat ccgttacatc acctgtttga aaatgatgtt
    11761 caccaaccac tttaaatcca tgacgtttat aaaatgcttg agcacgagga ttatgctccc
    11821 aaactcctag ccaaatttta tgtttattat gttcttgagc aattttttcg gccaattcta
    11881 tcaattgtga acctcttccg ccaccttgaa agtctttcaa aaaatatatg cgctgcactt
    11941 ctaaataggt ctcccccatt tcttcagttt gagcactatt aatattcatc tttatataac
    12001 caacattcgc accatcttct tgataaaaat aatgaaatga atctacatgg ttaatctctt
    12061 gtgtaaattt ctctacagta taattgtctt taaaaaattg atcaaaatct ttgtcatcat
    12121 agtaagaacc aaacgtgtca taaaatgttc tagttgctaa ttcaactaat tcactagcat
    12181 tttgttctga aatttctttg attatcccag ccatataaat cctccaataa acagtgatcg
    12241 aatcaaaata ttacttatgt tatttttcag ccaaaactat ttaaaaatac attaacacaa
    12301 atcaattaca aattgtattg attgtgtgta acatcaataa atgatacatt tattccagta
    12361 aaatggccgt attttcaaaa gagaaaaaga gaggatgtat cgttgtgata gaaacattta
    12421 aagcgtttgt aattgataaa gatgagagtg gtaaagtgac accaactttc aaacaattat
    12481 cgcctactga tttacctaaa ggagatgtgc tgattaaagt acattactct ggtataaatt
    12541 ataaagatgc tttagcgact caagatcata atgcagtcgt aaaatcgtat cctatgattc
    12601 caggaataga tttagctgga acaattgttg aatccgaagc accaggcttt gaaaaaggag
    12661 aacaagtaat tgtaacgagt tatgacctag gtgtcagcca ttatggcggt tttagtgaat
    12721 atgcgcgtgt aaaatcagaa tggattatca agcttcctga tactttaaca ttagaagaat
    12781 caatgatata tggcacagct ggttatactg ccggtttagc aattgaaaga cttgaaaaag
    12841 ttggaatgaa tattgaagat ggtcctgtac tcgttcgcgg tgcttcaggt ggtgtcggta
    12901 ctttagcagt actcatgctt aatgaacttg gttataaagt tatcgcaagt acaggtaaac
    12961 aagatgttag cgatcaatta cttgaacttg gtgccaaaga agttatcgat cgacttcctg
    13021 ttgaagatga tcataaaaag ccactcgcat catcaacttg gcaagcttgt gtagaccctg
    13081 ttggtggcga aggtattaat tatgttacaa agcgtttaaa tcatagtggg tcaattgcag
    13141 ttattggtat gactgccggt aatacttata ctaattctgt attccctcac attttaagag
    13201 gtgtaaacat tttaggaatt gactcggtat ttactgctat gaaattaaga cagcgcgttt
    13261 ggcgtcgtct cgcaaaagat ttaatgcctg aaaatttaca tgagatcaag caagttatta
    13321 catttgatga acttccagaa caacttaaca aagtaattaa acatgaaaat aaagggcgca
    13381 ttgttatcga tttcggtgta gataaatagt attcatgaaa aagacatccc gttatgcgag
    13441 atgtcttttt taatttagta tttgatatac ataccgcctg aatctggttc ggtaggtata
    13501 aatccaaatt ttgtatataa tttatccgct gggtagtctg caatcagact aacgtatgta
    13561 ctctcaacag ccacaccttt aatatattgc ataatatgct ccataattag actgccgtaa
    13621 ccttgacctt ggtaactttt caaaactgca atatcaacaa tttgaaaaac agttccgcca
    13681 tcgccaatca ctctacccat accaattaac cgatctttat catacaaggt tactgtaaat
    13741 aaggcattag gtaatccttt ttcagctgct tcgcgcgtct ttggactcat acctgcgtta
    13801 atccttaatg cgcaataatc ctcgcaagtc ggaatatcat atgtcacttt aaccattatt
    13861 taccccactt ttcatcacac aatatatcaa cctagtataa atgtttattt acaatagtct
    13921 tattcgcttc tttaaacact tcatgatgac ttgaaacata accctctgca ttcgcatctg
    13981 gttggatata tgttttagca aggttcgctg catttgcacc atcactaaat gcacttgcaa
    14041 ttagatgtga ttttgcatca tgataaacaa tatctccaca cgcatagata ccaggtatac
    14101 tagttgtcgt attaccaaat cctttaacac gacaatcatc atgcatatct agctttgaag
    14161 atgtttcact caataatgta ttacaacgat caaacccatg actaataatg acatcgtcaa
    14221 atttaactgt atgcctatcg ccactttcaa catgttccaa aacaacttca cttatatgcg
    14281 tttcatcatc attgccgacc aagtatttaa tacgtgtttt tgggcatagt ttcacattta
    14341 aatctgtcac caacgttttc atcgcttcat gaccacttac atcttctttt cgataaacaa
    14401 ctgtcacgct tttagcaatc ttggcaatat catgcgccca atctaatgct gtatttcctc
    14461 cacctgatat taatacatct ttatctttga aacgtctgta actttgtaca acataatgta
    14521 aattagttaa ttgatatctc tctacacctt taacatctaa ttgttttgga ttaataatac
    14581 ccgcaccaat tgcaatgata actgctttcg atgtatatat ttctcccgct tctgtttcaa
    14641 cttcgaaatg acgttctgcc tttttcctaa tatctaccac acgttcattc aaatgaactt
    14701 ccggtttaaa atataatcct tgcttaattg tatcttttaa aatttcatga caaggttttg
    14761 gcgcaatgcc gccaatatcc caaataattt tttcagggta aattctcatc ttacccccta
    14821 attcagattg aacatctatc aatcttacag acatatctcg caatccagca taaaagcttg
    14881 catacaaacc agacggaccg ccaccaatga ttgtaacatc tttcattatg tgcctcctat
    14941 gactctctat attcatttct ttcattaacg tgctcaaatt gataattatt atcatttaaa
    15001 gccattatac tattaatatt tatattgtta aaataaatcg catagttagc catgaattat
    15061 caataactat cccatcacaa tataaaaaca cacctttcaa agtaagaaac aacaatgttc
    15121 ctttgaaaga tgtgtatatt ttttagttct agttatatta ttttttaaaa gactcatcac
    15181 gtggttcttt aagaattgct tgtcttaaaa ggaaaaatag caacaataaa cctgcaagca
    15241 tacctgtgtg cccaatacct gcaaagcctg caaatgcttc tggagagtat gatttaccag
    15301 tgacttggaa gaatcctttt gtcaccatca tacctatagt aacgataaca cctatgttat
    15361 atacatagaa gaaccagtta aataagtagt aacttgataa tttaaataac ttctccaatg
    15421 gtaataatat taaaaacata aacataccta aaataagtgt atgtgtgtgt acaagtgata
    15481 attgtgttgt accagtaaaa tcatatgctt ttgttagctc tctatagaaa aagccactaa
    15541 ataaacctat gatcatataa aaaagaaatg aatataataa cctacgcatt ttgaatcctc
    15601 cctcaccttt tcattatatc cgattatacc tattataaca gtgacaaaaa agcgaagata
    15661 tgacacaact gaatttggtt tagcacatag tatgacagct aaaataactt tacatcaagc
    15721 gctatacaaa taaaatagct ataccatgac gctcgctaca aacgtcttgt atagctatta
    15781 tttctattta ttaaattatg acggcacttc agtacgtttt gtgctatgtt ttctaacaat
    15841 agctgaaact aaacttgata cagcgccaaa aatcataaac cctataaaca tccacattgt
    15901 actatttaat tcaatatgat gattcaagta taatagttct cttacacttg ttgcatagtg
    15961 tgtgaatgga ttccaatcat atacatattt ttgataactt tcaggcaaca tttgtttagg
    16021 taacgttaca agttgcatac taaagaacat taaaatgaag aaaattggaa ctgacttcat
    16081 acctagccat accataacac ctaaaataag gcccacgaaa ccaagaatcg caaatgctac
    16141 aaaaattgcg atacgatttg gatggtcaaa atcaaatctt tgaacgcctt gcatgaaata
    16201 aatataaaca aagctacctg caaatgctgc aacaactgca aatatcatct gtccaattga
    16261 agcaatgata cgatgttgca cgacgatatt gttactagtt ctaaatgcaa agaacaataa
    16321 gatagacgtt acgattgaac ctatccaaat tggcataaac attaggaatg gtgcattacc
    16381 acctgcttgg tgatctttaa ctttattaag tttttcatta tccactttta ctggattcgt
    16441 caaaccattg atgtccgcgg cattgacttt aacattttga ctcgttaatg tttccaaact
    16501 ttgcttcgta atttgagcat taatattatc acccatacca gttaacacag ctgatgcaat
    16561 ttgtgaacct tgtaagcttg atccttcact tacaatcgtt ttaaatttag cctgcttaac
    16621 ctctacttgt tgattcccca ttttttgttt catttgttga accacttgtg gcggaatttc
    16681 acctgaagca actttttgtt gcatttcttc ttttttacta tccataacta ctttttgtgt
    16741 tttactcata gcatcttttg aaaagtcttt atcaataata gctactccaa agattttttg
    16801 atctttcaaa tctttttcaa ggtctttttc actatcaacc ttaacccatt taattttatt
    16861 agaatcacta tctaataatt tatcctctaa ttttttacca atgttaacat ttttatcttg
    16921 aatcgttgta cctttatcct cgtttaatat accaattggt aaatcttttg gtttaggatt
    16981 atatgcagga taaaaagcta aagaaaagat taccaagata atcatagttg ctattggtgc
    17041 aatccataat aatttatttt taaatatatt cattttatta attacctcct tattagacaa
    17101 tgtgttgaat atttcgcatg ttgtctatta taatatgtaa gtgggaataa acaatacaca
    17161 atgttcatca atacgttcat taatagacaa atacacccac attgttcaat aacacaagga
    17221 ggttgattta tttttgaaag aagataggcg aattagaaag acaaagtcct ccattaaaca
    17281 agcgttcact aaattactac aagaaaaaga tttagaaaag attaccattc gcgacataac
    17341 aacacgcgct gatatcaata gagggacatt ttacttacat tacgaggata aatatatgtt
    17401 actcgcagat atggaagatg agtatatttc agaactaacg acatatactc aatttgattt
    17461 gttacgcggt tcttcaattg aagacattgc gaatactttt gtaaataata tactcaaaaa
    17521 tattttccaa catattcatg ataatttaga gttttatcat actattttac aattggaacg
    17581 cacgagtcaa ttagaattga aaatcaacga acatattaaa aataatatgc aacgttacat
    17641 tagtatcaat cattctatcg gaggcgttcc agagatgtat ttttacagtt acgtttccgg
    17701 agcaacaatt tcaattatta aatactgggt aatggacaaa cagcccattt cagttgatga
    17761 attagctaaa catgtgcata atattatttt taatggtcca ttaagaataa tggcagaaaa
    17821 ccgtttgcat aaatcaaacc ttgattcact aacttaagta atactcgctt ttcctaaatc
    17881 ctttagaaag gaactatata tcatgattac ttcattcaga cattctgaag atatagataa
    17941 acatattata aaaacaccat tagatcatac agcgtcatgg attaatgtag tagaaccaga
    18001 ccgagaagaa attgaaaatc ttatggaaca atataatata cctgaagact ttatacgtga
    18061 ccctttggac tcagaagaaa gttcccgtat tgaatatgac gaagatactg gttactcatt
    18121 aatcattatt gatttaccta tcgtcaattc aactaatcgt agcgttctat cttttgtaac
    18181 gattccatta ggcattatta ttggcaacgg tattatcgta acagtttgtg acgctgaaaa
    18241 tgaattttta gaaaatttac ctaagcgtga tattaattta aaatttcaca gcagatttgc
    18301 attagaaatt ttaactacta ttgccgacca ttataatcgt aacttacgat tacttaataa
    18361 aagtagaatt cgtattgaga aagaactaaa aaataacatt actaacaaac aacttttcaa
    18421 attaatggaa gttgaaaaaa gtttagtata ctttttagct gccttaaaag gtaacgatac
    18481 aattattaaa aagttattcc gtttacctgc aattaaacgt tttgaagaag atgaagaatt
    18541 actagaagat ttaatcattg aaaataacca agccatcgaa acaacagaat tacatcaacg
    18601 tatcttagaa agtatcacaa catcatacgc ttccttatta tctaatgata tgaatacgat
    18661 tatgaagaca ttaacacttt tcacggtact attaacgtta ccaacactcg tatttagttt
    18721 cttcggtatg aatgtgccgt tgccaattga tgaccatagt tatatatctt ggattattgt
    18781 ggtaggtatt tcattaattc ttgtcgtaat tgttagtatt ttcttatggc gtaaacagaa
    18841 attataacta aattaaaagg tatgacagct taaagtttca tcctattgca tttgcaatga
    18901 ctgatacttt gattactgtc atacctcttt ttttattcta caacttcttt atttgctttt
    18961 cttttagata aaattaaagt aactgttata gcaatgatga atgatattgt cataccaaca
    19021 aagtagtgta accatcctgc atgtactgga ttgattgaaa taaatcctgg caatccagca
    19081 gttcctaatg cgattgcttt aaccttgaag aaagcaatat atgctgaacc aatacctgat
    19141 ccaacgatag cgccaataaa tggatatctt agttttaagt taacaccaaa catagccggt
    19201 tctgtaatac caagtaatgc tgaaatacct gcggcagatg ccacaccttt taacttctta
    19261 ttttgtttaa taataaagaa cgctgcaatt gctgcaccac cttgtgcaac attagacatt
    19321 gtcgcaatcg ggaatataaa tgatccaccc gttttagtgg catctgcaat taatgtcgtt
    19381 tctacagcta taaagctatg atgcatacct gtaataacaa tcggagcata caataatccg
    19441 aatattaatc caccaattgc accaccaaat tcataaagcc atgttaaacc atctgataac
    19501 cagtaaccta attgtcgagt gattggacct acaaatgaaa atgttagaaa tgctgttata
    19561 aaaatagata acaatggtgt taacaaatta tctaacaccg ttggaataac tttgcgtaac
    19621 cctttttcaa ttgaggctaa gatataagct gctacaagca taggtaacac ttgtccttga
    19681 taacctactt gattaatatg caaaccaaaa acatcccaat atggaatagc cttacctgct
    19741 tcaactgctt ttgggaaatc gtatgcgctc attagcgatg gatgaactag tatcatacct
    19801 aatgcagcac ctaaaaatgg attgccacca aagcgttttg ctgcactaaa tccaattaaa
    19861 attggtaata atgtaaatgg tgcattcgca aaaacattta tcatttcagc taatccagca
    19921 aattgactat atacatctat caatgattta cctgaaaaga ataaatcttt cgcagtcaaa
    19981 atgttattta accccattaa taaaccacca gcaacgatgg ctggtataat cggaacaaag
    20041 atatctgaaa gcatttttac aaatcgctgt aacggattca tatttttagc agattgtgct
    20101 ttgacttccg aagtggttga tgcttctttt ccagttaatt tttccagttc actaaatact
    20161 ttattgactg taccagaccc aataataatt tggtattgtc ccccagtaga aaacgtccct
    20221 ttaactacat ccatattgtt tagcgcctct tcatttacta aactttcatc atttaaaact
    20281 aatcgtagtc tcgttgcaca atgcgccatt gcatctaaat tctcttctcc gcctatcgcg
    20341 ttcaaaattt cttctgcgga ttgcttataa ttcatatatt tagcctccta attagaaccg
    20401 gttccattta ttccacttta ttgtaaccgg ttccaaaaat atttgcaaca ctctattatc
    20461 attttatata aacacctaca ccatttttga tagcgcattc atttttattg ctatactaca
    20521 tgcatgtata ttaacattaa ttatcggagg taattttatg aaagaacgtg gctatatcga
    20581 acagctttgg cgtgaggaaa aatatcatgt attgttacac agtcaacagt cttaccaaat
    20641 gataaggaat gctttaaaaa cggatttatc acttcatcaa gttcaacaaa tgattgatgt
    20701 tgctttgtta attgaaccga gtataggaag tgtatgtaat gcttttgatc atatgtgggg
    20761 atatttaaaa aaatgtgcga atgaagaaga aagacaacaa tcaaaactac ttaaagctga
    20821 ttttattaat ggtaaaatcg acacccaaac tttattagat tttttagcag aacttgctaa
    20881 taaatatgat gttcaatatt tactacaaag tcgtgtttta aacacaaaaa gaaagagata
    20941 gattgaaact ttagttgtaa cggacaaata gtagtcattg gagttcagca attcatactt
    21001 tgtactaaag tttactataa tctatctctc aaggtttaaa tgtacatatc tatattaaaa
    21061 tgcgtttcat gatgttagaa gataccatac acatttaatt agttacagca taaatcaatt
    21121 tataccctta attattacat tgtttatatt ttaaattcgg gggagtaatt ttaagtaata
    21181 tcttgttgct gctaattcta ttgttaataa attgatatta gatgtagaaa ttgagtgtga
    21241 aagttaataa tagatgattt agctagtaac ataattaatt catatatagt caaaaagttc
    21301 tgttagatac tgttcttgat gaatattctt tgatgttagg tgttagctat tctagatgag
    21361 tttcttagat gtggatgcct gtcttgatga agttatctat gatgttaata taatacttga
    21421 tgttgattct tagatgttag tgcgatttca agatgctgat tctttgttgt tgcactatct
    21481 tggtgacgtt tctagttgtt tatagttatc gcatctcttg atgtaaaatc taagatgttt
    21541 atgcatttct agatgttaat tcttcgctgc ttaagaatta tcttgatggt aaatctagtt
    21601 gtgccatgca ttttctcatg atgaatctaa ggtgttaatt cattgatgtg tgctgtttct
    21661 tgttgcttca ttatcttgat ggtgaatttc gttgtctaat gcactttttc aaatgattat
    21721 tctaattgtt acagcaataa ctagatggat tttcttagat gttcactttt ctagatgttt
    21781 atacttatct tgatgttttc taaacagaac ttttaaacgc tttatttttt agtagattgt
    21841 ttaaaatttc ttagttgatg gttattgttg ttgatttgaa taatttgtag cgaatatttt
    21901 gtaacagggc tactaagaat ttttataaca agaagtgatt tcaatcgtac taattttcag
    21961 cgtttgatga caaaagtgta tgtccaaact taaataatgg cgataaaatt taagtcatgg
    22021 gtacatttaa tgttttttga ttcaaacttc cattttggtc aatttgcttt ttttataaca
    22081 tagttgaata tcaataaaag attagcaagt tagttcaaaa tggctatgta ttgttttttg
    22141 aaatacatag gtttgaaatc tatat
//
LOCUS       DALAAR010000046        21165 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000046 DALAAR010000000
VERSION     DALAAR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.048"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            157..834
                     /gene="recU"
                     /locus_tag="O2458_001774"
     CDS             157..834
                     /gene="recU"
                     /locus_tag="O2458_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCZ3887637.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            831..3014
                     /locus_tag="O2458_001775"
     CDS             831..3014
                     /locus_tag="O2458_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ3887638.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(3580..3921)
                     /locus_tag="O2458_001776"
     CDS             complement(3580..3921)
                     /locus_tag="O2458_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887639.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(3926..4585)
                     /gene="nth"
                     /locus_tag="O2458_001777"
     CDS             complement(3926..4585)
                     /gene="nth"
                     /locus_tag="O2458_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCZ3887640.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(4575..5261)
                     /locus_tag="O2458_001778"
     CDS             complement(4575..5261)
                     /locus_tag="O2458_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ3887641.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            complement(5589..6881)
                     /gene="asnS"
                     /locus_tag="O2458_001779"
     CDS             complement(5589..6881)
                     /gene="asnS"
                     /locus_tag="O2458_001779"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCZ3887642.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(7203..9896)
                     /locus_tag="O2458_001780"
     CDS             complement(7203..9896)
                     /locus_tag="O2458_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCZ3887643.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(9920..10891)
                     /locus_tag="O2458_001781"
     CDS             complement(9920..10891)
                     /locus_tag="O2458_001781"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCZ3887644.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(10878..12080)
                     /locus_tag="O2458_001782"
     CDS             complement(10878..12080)
                     /locus_tag="O2458_001782"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCZ3887645.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(12085..13227)
                     /gene="bshA"
                     /locus_tag="O2458_001783"
     CDS             complement(12085..13227)
                     /gene="bshA"
                     /locus_tag="O2458_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCZ3887646.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(13468..13785)
                     /locus_tag="O2458_001784"
     CDS             complement(13468..13785)
                     /locus_tag="O2458_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ3887647.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(14122..14802)
                     /locus_tag="O2458_001785"
     CDS             complement(14122..14802)
                     /locus_tag="O2458_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCZ3887648.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(14874..15461)
                     /locus_tag="O2458_001786"
     CDS             complement(14874..15461)
                     /locus_tag="O2458_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCZ3887649.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(15451..16026)
                     /locus_tag="O2458_001787"
     CDS             complement(15451..16026)
                     /locus_tag="O2458_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCZ3887650.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(16040..17284)
                     /locus_tag="O2458_001788"
     CDS             complement(16040..17284)
                     /locus_tag="O2458_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887651.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(17291..18589)
                     /gene="aroA"
                     /locus_tag="O2458_001789"
     CDS             complement(17291..18589)
                     /gene="aroA"
                     /locus_tag="O2458_001789"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCZ3887652.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(18599..19663)
                     /locus_tag="O2458_001790"
     CDS             complement(18599..19663)
                     /locus_tag="O2458_001790"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCZ3887653.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LVFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(19689..20855)
                     /gene="aroC"
                     /locus_tag="O2458_001791"
     CDS             complement(19689..20855)
                     /gene="aroC"
                     /locus_tag="O2458_001791"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCZ3887654.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         6851 a         3803 c         2957 g         7554 t
ORIGIN      
        1 aaacttttca attaattaaa attgtgtgta aaattccaac attaacggta aatgtattat
       61 aatattattg tgttaatgct gaaatcacgt taaaataagc tttggtgtat tgtgtctttc
      121 gcatttatac taatttaagt ttggtgtttc actataatgt tgtgtataat aaacaacgtt
      181 tactaccaaa cttgtgtggt gaaatttatg aattatccaa atggtaaacc atatcgtaaa
      241 aatagtgcta tagacggagg gaaaaagacc gctgccttta gtaatattga gtatggtgga
      301 cgtggtatgt cacttgaaaa agatatcgaa cattcaaata cgttttatct taaaagcgac
      361 attgcagtta ttcacaaaaa gcctacgcca gtacaaatag ttaatgtcaa ctatcctaag
      421 cggagtaaag ctgtgattaa cgaagcttat tttcgtacac cttcaacaac tgattacaac
      481 ggcgtttatc aaggttatta tattgatttt gaagcaaagg aaactaaaaa caagacgtcc
      541 tttcctttaa ataatattca tgaccatcaa gtcgaacata tgaaaaatgc atatcaacaa
      601 aaaggtattg tgtttttaat gattcgtttt aaaacgctag atgaagttta tcttttaccc
      661 tattcaaaat tcgaagtatt ttggaagaga tataaagata atattaaaaa gtctataaca
      721 gttgatgaaa tacgaaaaaa tggttaccat attccttatc agtatcaacc aagattagac
      781 tatctaaaag cagttgataa gttgatatta gatgaaagtg aggaccgcgt atgacggaaa
      841 acaaaggatc ttctcagcct aagaaaaacg gtaataatgg tgggaaatcc aactcaaaaa
      901 agaatagaaa tgtgaagaga acgattatta agattattgg cttcatgatt attgcatttt
      961 tcgttgttct tttactaggt atcttattgt ttgcttatta tgcttggaaa gcacctgctt
     1021 ttaccgaagc taaattacaa gatccgattc ctgcaaagat atatgacaag aacggagaac
     1081 ttgttaaaac attagataat ggccaaagac atgagcatgt aaatttaaaa gacgtgccga
     1141 aatcaatgaa agacgcagta cttgcaactg aagacaatcg tttctacgaa catggcgcac
     1201 ttgattataa acgtttattc ggtgcaattg gtaagaactt gactggtgga tttggttctg
     1261 aaggtgcctc aacattaaca caacaagttg ttaaagatgc atttttatca caacataaat
     1321 ctattggacg taaagctcaa gaagcatact tatcatatcg tttagaacaa gagtatagta
     1381 aagatgatat cttccaagta tatctaaaca aaatttacta ttctgatggc gtaacaggta
     1441 ttaaagctgc tgctaagtat tactttaata aagatttaaa agatttaaac ttagcggaag
     1501 aagcttattt agccggttta cctcaggttc caaacaacta taatatttat gatcatccaa
     1561 aagctgctga agatcgtaaa aacactgttt tatacttaat gcattatcat aaacgcatta
     1621 cagataaaca gtgggaagat gctaagaaaa tcgatttaaa agcgaactta gtaaatcgta
     1681 ctcctgaaga acgtcaaaac attgatacaa atcaagattc tgagtataat tcatacgtta
     1741 actttgtaaa atctgaatta atgaataata aagcattcaa agatgaaaat ttaggtaatg
     1801 tattacaaag tggtattaaa atttatacaa acatggataa agatgttcaa aaaacattac
     1861 aaaatgatgt tgataatggt agcttctaca agaataaaga ccaacaagtt ggtgcaacaa
     1921 ttcttgatag taaaactggt ggtttagttg ctatatctgg tggacgtgat ttcaaagacg
     1981 tcgttaacag aaaccaagca acagatcctc accctactgg ttcatcttta aaacctttct
     2041 tagcgtatgg acctgccatt gaaaatatga aatgggcaac aaaccatgcg attcaagatg
     2101 aatcttcata tcaagttgat ggttctacat ttagaaacta tgatacgaag agtcacggta
     2161 ctgtatctat ttatgatgct ttacgacaaa gtttcaatat cccagcttta aaagcttggc
     2221 aatcagttaa gcaaaatgct ggtaatgatg cacctaagaa attcgctgcc aaacttggct
     2281 taaactacga aggcgatatt ggtccatctg aagtacttgg tggttctgct tcagaattct
     2341 caccaacaca attagcatca gcatttgctg caatcgctaa cggtggtact tataacaacg
     2401 cgcattcaat tcaaaaagta gttactcgtg atggtgaaac aatcgaatac gatcatacta
     2461 gccataaagc gatgagtgat tacactgcat acatgttagc tgagatgcta aaaggtacat
     2521 ttaaaccata tggttctgca tatggccatg gtgtatctgg agtaaatatg ggtgctaaga
     2581 caggtactgg tacttacggt gctgaaactt attcacaata taatttacct gataatgcag
     2641 cgaaagacgt gtggattaac ggctttacac ctcaatacac tatgtcagtg tggatgggct
     2701 tcagtaaagt taaacaatat ggtgaaaact catttgtggg acatagccaa caagaatatc
     2761 cacagttctt atatgaaaat gtgatgtcaa aaatttcatc tagagatggc gaagacttta
     2821 aacgtcctag ctcagtaagt ggtagtatcc catcaatcaa tgtttctggt agtcaagata
     2881 acaacactac aaatcgtagt acacacggtg gtagtgacac atcagcaaac agcagtggta
     2941 ctgcacaatc aaataacaat actagatctc aacaatctag aaacagcggt ggattaacag
     3001 gtatattcaa ctaatccact caacataaaa tcctcagtta taccatattt atggtgtagc
     3061 cgaggatttt tttaggttct tcatctttta tggtgggaag gtaaaacttc ctgctttttt
     3121 tataagtatt catacgctat tgctatatta gtagaaaccg taaaatcagt cattcacata
     3181 cattttgaaa catctaaata gtttttcata ttcacatctt tagctatgta aaattattga
     3241 atttgaataa gtggttgttc ccatttttaa aattatgatt atattttctc atagtcatct
     3301 tagacgctgt tttaaattga tatatttatt aattccctgt gtacgttaat cagtgaaata
     3361 tcaacatgtt tgcaacatat tttcacaatc atagatataa gaaatccacc tacgattcga
     3421 aatagatatc ttaaagcata tctcaagata tccattatga tgtagatgga cagtttttat
     3481 attaagacgt tgtaatgaca cgacacctaa cataattata aatatgtgtg catgaattag
     3541 ttttatatat tttcaacatt aggaaatgat caatcttgtc tatacctgct tattctttaa
     3601 ccttgtttga taactagcat tcattttaat caattcagtt ttaaaggtct tcatttgtcg
     3661 atagcccata aaatgatatt tacgttcaat catataatca tatctttctt taaaattcat
     3721 tggcacaaca ggatattgat ttaatttatc aaaatcaata ttgccagtta tgtcattaac
     3781 ctgcttaaaa taatcaataa ttttactatg gtattcatcc aagtagggct ttgcttcact
     3841 agaacctaat gtcttatttt tggcaaataa atcaatctct tgttctaaat tatcaaatac
     3901 ctcttttgta atatttgtca tgttatcacg cttctttcaa actagcttta taacgttttt
     3961 gtccttctct acaatcttct aatagtggac aaatatcgca tttaggtttt cgggctaaac
     4021 agtggtatct tccaaagaaa atgagttgat gatggctcct attccatcta tctctaggta
     4081 tgacagaaca taaacggtct tctacctgtc tcacattatc tttccaacga ttaataccta
     4141 agcgtttaga aacacgttct acatgcgtat caacagctaa tgaaggttca tcaaatgcta
     4201 cactcatgac tacattagca gttttacgtc ctacacctgc taaactttct aattccttat
     4261 gtgtttgtgg tatttctcca ttaaattgat caatcaaaga ttgacaaagt ttcttaatat
     4321 tcttagcttt gttacgatac agaccgatag aacgaatatc attcataagt tcttcatcac
     4381 tgactgccaa ataatcttca ggcgttttgt attttttaaa cagctcagtt gttactctat
     4441 ttactagaac gtctgtacat tgcgctgaca ataatacagc aatagttaat tcgaacggat
     4501 tatcatgttt taattcacat tctgcatccg gaaacatatt tgctataaca tcaatcattt
     4561 ctaatgcttt tttcttactt accatcaagg ttctccccgt ttaaccaatc aaatttaggt
     4621 accgttttaa ctgtgtgcgt cattttcggt ttattgaatt tttctcttat ttttctagaa
     4681 tcgtcaattg ttttgacatt gtttttcttc caattaagta aaatacgatc tatatattta
     4741 aagctaagtt tattcaaact attcgcctcg tctaatgccg cttgtataat tgcagtatcg
     4801 tgtttatcaa catcaatcca ttgatttaac gtttctattt catatggaga taacggcctt
     4861 gcaaatgtat cctctaaaac tctaaataat tgtttaaatt tttctttact atttagctct
     4921 ttcgtttcca tactttgttg cttcaatata tgacttaatt tttcgaaaaa aggatctaga
     4981 ttcatatatt cggtaaatct accttcttca tctttttgaa cttgtaattc tagcaattca
     5041 cgttgtatca aattttgaat aaccattgta atatcacgtg gttgcatagt tgagcccttc
     5101 tgaagcaatt caattgaagg ctgtttattt gatgtttcgg aagcataaat caatttaagc
     5161 aaaatgacta aatcttgctc atctaaacct aagtcactgt aatggtctaa taattctctt
     5221 cgtatcacta caggtcttgc ttttaattga tatttatcca tgttcgtgcc cccttttaaa
     5281 tttaaccatt acttaatagt ataaatcatt ttctttaatt ttcgactaaa atgcttaata
     5341 tatttaacta gtagcaaaca aattttaaat aacagtattt tttaacacta gattttaaca
     5401 atgaacttta tggataatca gatgaactat ttattttcca aatatagtta ctaaacaaaa
     5461 gtttgttcat gattaatata agccgttatc aatcttcatt ttccaagaat aaatcgccca
     5521 gctattaata taggctggac gattcttttt attatcataa tatatatttt aagtaacgac
     5581 cctaaaactt atggatataa acggtttaat aatcttggga atggcgctgt ttcacgaacg
     5641 tgttcaacac cagaaatcca tgctactgta cgctctaaac ctaaaccaaa tccacagtgt
     5701 ggcacactac cataacgacg taagtctaag tagtaactat atgcttcttc gtctaatcca
     5761 tgttctttaa cgcgttgttc taacaattct aagtcatcca cacgttcaga tccaccaata
     5821 atttcaccgt atccttcagg tgcaattaag tctgcacata atacagtttc ttcattttca
     5881 ggatttggtt gcatatagaa aggcttaatt ttagttggat aattagtaat aaacaccggt
     5941 aaatcataat gattagcaat ggctgtttca tgtggcgcac caaaatcttc gccccattca
     6001 atatcatcaa agccttctgc ttttaagaat tcaattgcat catcatatga aattctaggg
     6061 aatggtgtcg caactttttc aagttttgat gtatcacgct ctaaaatttt caactctagt
     6121 ttacaatttt ctaaaactga ttttacaaca tgtgttacat attgttcttg aatttctaaa
     6181 ctttcagcat gatttgtgaa agccatttct ccttcaatca tccagaactc gatcaagtgt
     6241 ctacgtgttt ttgatttttc agctctgaaa gttggaccaa atgaaaatac ttttccgtgt
     6301 gccattgctg cagcttctaa gtataactga ccactttgag ataaaaacgc atcttgatca
     6361 aagtatttag tatggaataa ttcacttgta ccttctggcg cacttgctgt caaaattggt
     6421 ggatcaacct ttgtaaatcc atctttgttg aaaaattcat acgttgcacg aataacttca
     6481 tttctaattt tcattacagc atgttgtttt ttagaacgta accataaatg acggtgatcc
     6541 attaagaatt ctgtaccatg atttttaggt gtaatcggat agtcatgcgc ttctgaaata
     6601 acttcaattg atttcacttg catttcgtat cctaagtcag aacgattatc ttctgtaatt
     6661 gtgcctgtaa cgtatagaga tgattcttga gtaatttctt tcgcaagttt gaatacctct
     6721 tcatcaactt ctgatttaac tactacgcct tgcataaagc ctgttccatc acgtaattgt
     6781 aaaaaggcga ttttaccact tgaacgttta tttgttaacc aagcaccaat tgtaacgtct
     6841 tggtttaaat gatctttcgc ttgtttaatc gttgttttca taaccattct cctatttatt
     6901 ttttcgttat acaatactca ttcattttaa caaaatccgc tttcaagttc tagaactaga
     6961 ctaaaagata acgtgtaaat ggtaatgatt tacgcacaga tttgaacatt aatttatatt
     7021 aagacaatac aatcatcagt ttcagacaaa atatataata aagcctcaat attattagta
     7081 tgaaggtcgt tttttgtgct aacattcaag tttaattata aaactcaaat tttgacgacc
     7141 aaacaaaaat tcttgtaaca gaacatagca catgtcatgt cacaagaatt ttactttgta
     7201 tttcactttt tctttttttg aatttgtctt aataattttc caaactgttg aatgtcgcct
     7261 tttttctgac gataattttc aagtgtttgt tcaaaaaagt ttttataatt actgtttatg
     7321 agtcgatcat caaatgaaac tattatgccg cgatcatttt catttctaat taatcttcca
     7381 agtccttgtc taaaacgtgt aactgcatca ggtaatacat attccttgaa agttgaagtg
     7441 aattcagaat ccataagcca atattttgca ttatgcttgt tcataaacgg taacttcgct
     7501 atcatcacac atttaatacc atttgcttga aaatcaaaac cttcaaaaaa tgttgacgta
     7561 ccaagcagta tggccttatc aaaattatta aactgttgta ctattttata attttggttc
     7621 tgctgttgtg ttaatacaac ataatcttca aattctggca attcatttag catatcttgt
     7681 accatatgca tcattttata actcgtaaat aagactaaac attttgatga cgttatagtc
     7741 gtatattcaa taatatagct tacaatcgat gctacatact catctatatt tttatattga
     7801 taagatgcta catcactcgg tataaataca cttgtatttt ttgcactttg taacgacgtg
     7861 ttaacttcaa atgtattaaa gtgaacatct ttgttgaata actgtttaaa agcttcaaac
     7921 gaatgattaa attttaaagt accagatata aaaatgagcg atttaaattt ttccaatact
     7981 tgtttcgtta atacatcttt tacagcgtaa tctttcacat ataaacgtat tgttgatttt
     8041 tgagataaat ttttaatcga aatgaaacta gtatgtccag cttttaaact ttgttcgata
     8101 tttttaaatt tatcttttaa atacaacaac tgtttccgta atgatttaac tgttttatga
     8161 ctaatgccat tgaatatttc tagcgtttta tttaacttat cgataatcgc atgtaaatcc
     8221 ttcaaaatgt cttttgtttc aaaagtaaat acattatgga agcgatgaat atcatcatca
     8281 taaacatcag aatcattgat aatcgtaaat atcgttgaga acaattgctc atttaactca
     8341 tgaatctcat tcatactagc cttcaagcca aaaatatcaa ttggtgcaat atctaatttt
     8401 tccaaaattc gctgcttttc cagttgatca attgccttta acaatttttc attttcgttt
     8461 ttaccaatca aaccaagctg atatttaata tcagcataac tcaactcatt tgtcacttga
     8521 tttaaggcat agtctggtaa gcgatgtgct tcatccacta tacaatcatc aaacaattga
     8581 tatattgaat tttcaacatc agaatgaatt aaatgtgcat gatttgtaat accaatttga
     8641 atgttctgtg catttcgctt aataaaatta taataatgaa catcgtgacg tgccggtaca
     8701 tatgtttcaa ttttctggtc aaaatacatc ttttgaccac cttttaaatt taattcctgt
     8761 atatctccgg acggcgtctc tgtaatccaa atcagcaatt gcattttcaa gatattcact
     8821 tcgtaattac ttgtgtcatc ttttaaaatt tgactaataa gccccaatga aatgtaatca
     8881 cttttacttt taatcaatag tgcattaatt ttaaaattca acgcttcatt cattgctgga
     8941 atatcttttt ctaacaattg actttgcagt aatttagtat tggtagaaat catgacatgc
     9001 ttcccagttt caatattata catcaaggcc gcaagtaaat atgctaatga tttaccactg
     9061 cctagtgatg cttcaatcat tgctttttca ctatgcatga gctgatctaa tatagtttcc
     9121 gctaaatata attgttgcgg tcgatatgtt aagccaagtt gatctacagc tttgctatat
     9181 aaagacttca agctgccatt ataatttgtt gtcggctttt taaaatcaac ttgcttacga
     9241 tagataatct gttcgaactt ttcgtacgat ttatccaatg gctttgcatc atattgccta
     9301 accatctcaa agaaaatatc atacaaatcg tatttcaact gtttacttaa ataatataat
     9361 tgcttcaaag tatctaacgg taacttttca aatttttcaa aagctaatat catcaattta
     9421 gcagtagtag cggcatcttc gtcagctcga tgggcatttg ctaaggtaat accatgtgcc
     9481 tctgctaatt cacttaattg atagctttta tctgtaggaa aagctatttt aaagatttct
     9541 agtgtatcta taactttttt gggacgatat tgaatattac aatctttaaa tgccttttta
     9601 ataaaattca aatcaaaatc tacattatga gctacaaaaa tgcaatcttt tatcttatcg
     9661 tagatttctt gtgcaacttg attaaaatat ggcgcttgtt gtagcatatt ttcttcaatg
     9721 gatgttaacg cttgaatgaa cggcggaatc tctaaatttg ttctaatcat agaatgatat
     9781 gtatcaataa tttggttatt gcgcacaaac gttataccaa tttgaatgat atcgtcaaaa
     9841 tctaattggt tgcctgttgt ttccaaatcc acaacggcat aggttgccat acccatagct
     9901 atctctcctt gctttagtgt taaaaatcta tatctgcact aattaaacgg tgtgattcac
     9961 ccgcttcatc tctaacaatt agatagccat cgtaatctaa atcaattgct tgtcctttaa
    10021 actgtttatc attttctgta aatagcaacg ttctattcca aatattagaa gctgcagtat
    10081 attcttcacg aatttcagaa aaaggtaacg ttaaaaattg attatatctt ttttcaattt
    10141 cttgaagtaa tatctctaaa aattgatatc tatctaattt atttttatca tgtaattgta
    10201 tacttgttgc tctatgtcta atactttcat caaagttttc tagttgttgc gtcaaattaa
    10261 tacctatacc acatattatt gcttctatac catcattatt agcaaccatt tcagttaaga
    10321 aaccacacac tttaccatta tcaatatata tatcattcgg ccatttcact ttgacttcat
    10381 cttgactaaa atgttgaatc gcatctctta tccctaatgc aataaataaa ttaaatttag
    10441 atatcattga gaatgcaacg ttaggtctta acacgacaga catccaaagt ccttgccctt
    10501 ttgaagaact ccaatgtcta ttaaatcgcc cacgaccttt cgtttgttca tcactcaaga
    10561 taaaaaatga agattgattt ccaacaagtg actttttcgc agcaagttgt gtagaatcta
    10621 ttgaatcgta tacttcacta aaatcaaaca aagcagaact ttttgtatat tggtctatta
    10681 taccttgata ccaaatatct gggagctgtt gtaataaatg ccctttatga tttactgaat
    10741 ctattttaca tccctctaac tttaattggt caatcacttt ttttactgca gtgcgtgaaa
    10801 tattaagtga ttccgcaatg ctttgtccag atatataatt cggtttattt ttatagagta
    10861 attgaagtac atcttgacta tattttgaca tgattatcca cccatttcaa aatttcagtt
    10921 tcttcgttgc ttactttacc tgttacaatc gctatctcaa tttgtcttag cacatctttt
    10981 aaccacggac cacttttggc atttaaatgt gccataagta caccgccatt aaccatcatg
    11041 tcttttctat tatgcatagg taaacgatgt aatgtttcat caatcgtttg aaggttaacg
    11101 attaatggtt catgtccttg aatatcattt gcctttaaca cgtctgctgc aaccattaca
    11161 tttttaatga gatgcgtatc ataatcataa acaaacattt ttaattgttc ttttgtaata
    11221 atacttggta atgcattcat aatttgaata tattgattga tatcttttac ttgtcggtta
    11281 cttagcttta aaggcttcaa tgagtaatta atatcaaatt taactgatac tatagcaatc
    11341 aacaattcta aatcaattgc ttcagttaca ttaatttgat tcatatcaag atgttcgaaa
    11401 tacggcatat aattaaatgc tttcagcgat tttaaatgat taaaactctt ttcaacatta
    11461 atacctcgca ttaatttagt tagttcaatc actatacgct caattgataa aaatttaata
    11521 tctgccattt gtatacgcat cgcttcgaat gtttccgttg caatatcaaa tgataattgt
    11581 gactggaacc ttaaacatcg aatcatacgt aaagcatctt cttggaatcg ttcctcagct
    11641 atacctacag ttcttattat tcgattatta atatcttgtt gaccatcaaa ataatcatac
    11701 aatttgtatg ctgtatccat tgctatcgca ttcatcgtga aatctcgtcg ttgcaaatct
    11761 tcgtataaat cacgaacaaa tgtaacacca cttggtctac ggtgatcgac ataatcttct
    11821 tcagcccgga atgttgtcac ttcataattt tcatcattaa aaactacatt tatcgtgcca
    11881 tgttctttac ctacaggtat cgtatgacta aagatagatt ctatttcatc cggcgttgca
    11941 cttgttgtga tatctatatc atgaatattt cttcccatga cataatctct tacagagcca
    12001 cctacataat atgcttcaaa accattgtct tgaatttgtt ctaatatagg ccttgcctgt
    12061 tcaaataatg atttatccat attattactc gcctttactt ttgttatgct catttagcat
    12121 tttttgataa taatactcat attgatctgt aataagttct gatccaaaac gttcagcaat
    12181 atctgctagc atgtttttct gaagtttgtt gtataacacc ttatcttcaa gtaatcggat
    12241 agcatagtca ctcgctgaat cacaatcacc cacatctacg acaaatccag tttcaccatg
    12301 tttaataacc tctttaattc caccggcatt tgaaccaatt ggaacgacgc ctgttttcat
    12361 agcctcaagt aaagttagtc caaagctttc tttttcactt aataataata ctaagtcaga
    12421 taattggtaa aattcactta cgcaatcttg tttccctaaa aataaaacat cctcttctac
    12481 gtttaactct ttcgtcaatt gacgcattgg cactaattca ggaccatctc caagtaaaat
    12541 taatttacta ggtatctttt cacgtacttt tgcaaatgtt tctataatag tatctatgcg
    12601 ttttacttgt ctaaaattcg atacatgtat taacactttt tcatctggtg ctataccaaa
    12661 ttgtgatttt aatgctgtgt tatgtttagt tggaaactca ttttcacgta caaaattata
    12721 aatcggtata atttctttgt tagtttcaat aatttcatgt gtttcttgtg ctaaagattt
    12781 actcacactt gtcacaatat cacttttttc aatgccaaat ttaattgcac cttggagtga
    12841 atgatcatag cccaaaacag taatatcagt accgtgtagc gttgtcataa tttttatatc
    12901 tttacctgac atctcacgag ctaaaatccc acaaattgca tgaggtacag catagtgcat
    12961 atgcaacaaa tcaagatcat attctttaat aacttcagcg attttagtac ttaacgtaat
    13021 atcatacggt ggatactgaa atactgcata ttgattcact tcaacttgat gaaaaatcat
    13081 attcggtaat ggttttctta ttctaaacgg gatatttgaa gtgataaaat gtacttcgtg
    13141 acctcgctct gctaatttaa ttcctaattc tgtggcaata attccagaac cacccatgga
    13201 cgggtaacat gttataccta tcttcattcg cttgcccatc ctttctttct atttctctct
    13261 attattctcg atgcgtagat aattgtttaa atttaagttt atagtaatgt tgagtttata
    13321 atttcatata tctaaaaaca ggtgttgtat atataatcat tcatctagtt atacttactt
    13381 taaaaataat ataatttcat gcgatgcaat tcattgatgg atgtttttaa tcttaatcaa
    13441 atccaaataa agcatatatt tttaaattca ctttctttcg aatcgatttt tatctcttgt
    13501 attaaacttt tccattgttt cattaaagct ctctgtcata tctattccca ttgaattcgc
    13561 taaacataac aacacaaata aattatcacc taattctgct ttaatcgtat ttgcttcctc
    13621 tgaatctttc ttcttttttt caccataggt atgatttatt tcacgtgcaa gttcgcccac
    13681 ttcttcagtc aatctagcta agttagctaa tggtgaaaaa tatcctgttt taaattgtcc
    13741 aatatattca tcaacttcac gttgcatttc taccattgat ttcatttcta cgttctcctt
    13801 atattgcatt tctaatatag tatatatcaa tttgaagtct catgcatgtt tagtgcaatc
    13861 tactgactct agctaaacta gatgacaagt tgtttatttt agaagtttaa taattcagtt
    13921 tatataaatg taatgcattc ctaactaaat taaatcaatt gaaattggga ttataacttt
    13981 atgatacgta ccactacaat aaaataatat agtgaataat ctaccattag aaaaataagc
    14041 acaaaaaaac tagcaaccac acaaaaatgt gattagctag ttaataagtg tctaatttaa
    14101 gttaattgtt aatctataag attaatcact tgaacgcgca atcaaaataa tacgtacaag
    14161 ctctgctaca gcgactgcag ttgctgcaac ataagtcatt gctgctgcag ataatacttt
    14221 acgcgcatgc ttgtattctt tttcatttac aatgttcaat gccgtaattt gtttcatcgc
    14281 tcttgaactc gcatcaaact caactggtaa cgtaacaatt gagaataata ccgctaatga
    14341 cattaaacca gcaccaatcc ataaagcagt tgaaccaaat gcactaccta tcgctgttaa
    14401 gataatacct aacatgatga tcatataact taatgaactc cctaggtttg caacaggtac
    14461 taatgctgct ctgaatctta agaaccaata tccttggtga tcttgaatgg catgaccaac
    14521 ttcgtgggct gcaattgcag ttccagcaac tgatggtctg tcatagtttg caggagatag
    14581 tgaaacaact ttctttttag gatcgtaatg atctgttaag aatccttcac ctttaacaac
    14641 ttcgacatca taaataccgt ttgcatgtaa aatttctaat gcaacttcac gacccgtttt
    14701 accactagtt gatctaactt gtgaatattt ctcatagtta gatttaactt tgtgttgtgc
    14761 ccataaagga agcaccatta atattacgaa ataaattatc atagtaaaaa ttgaagacaa
    14821 taaactcact ctcctttata aatattttac tgtcatttgc cgtttttatc aaatcattta
    14881 cactttaata atttgtttaa ttcaatataa agcaaaagtc caaaaacact tagacaacat
    14941 gataatacac caatttgcca cacatgtgta gttataaaat cataatatgg aaattgaagg
    15001 tgaaaatagt caatataatc attcaaaaac acccaaatca tcgctacact gattccaatc
    15061 atagaacgtt taaacctagg atagaagtaa attgcctgaa cagccattat actgtgggaa
    15121 aacattaata ccaaaccatt tactgtaata tcaccttgtt caataataaa taatatattc
    15181 attataactg cccaaatccc atatttgaat aatgttacaa atgccagtgc atcgataata
    15241 ctattttgtt tttgaattaa tatcaatgag atagaaataa ctaagtataa tattgcagtt
    15301 gggctatctg gaacaaaaat cttaaaatgc cagggcgtat gacttaattg ttcaccatac
    15361 catatataac cataaatcat ccctaatata ttacaaatga gtagcatcat taaccaagaa
    15421 cgttgataaa gtgtatattg ccaaaatgct ttaattgtca tctgctaagt cctcaaattg
    15481 attatgttta tttactagct tgagtgtatt taaaatttgc gttagttgat aaaaacgttg
    15541 cttttcattc atctgtaaac ttaaatcaat attgtgtaac aagtaatcta ttaataacgc
    15601 atgtttatgc cgatctatag ccatactatt taagtcatga agataagttt gataactggg
    15661 cgattccgtt agttgagcaa ctattaaatc atctagcctt tgctcacgct ttgacacgtt
    15721 tgcgaagtga atttgaatat caaaagcaca gttatgatta gcgatataat caaatatttc
    15781 atttgtattc attaacttta tattacgctt agtaaattga attgcagaag cgtgacttcc
    15841 cacttctgca atttctaatg tttcatgatg attaattttt gtatctacaa aatgaatgtt
    15901 tgccaatttc gcctcattca cttttatata gttaagcacc caaactgcaa tacgcgactt
    15961 aaatcgatat tgaaaaagta aatattcaat aaaactttct ttaatttgat tgaatgtctc
    16021 tgacatcaaa taccccattt taagattgca atcttgataa ttcgtcatgc caattttcgt
    16081 tacttggctc tagttccaac aattgattta aaatagtaat tgcttgttcc ttttgaccaa
    16141 tttcaattaa atagaaataa taatcactca taaaatcaat atttgttttc atcgttggat
    16201 atgctaattc aaagaaatgt tgagcttctt tatctcgctc ttcttgacca taggcgaacg
    16261 ctaaatgcca catgaatgta ggatccaaat cttcttcatc tacatatgtt aataattcaa
    16321 tgattgcttc ataatcttct tcattacgat ataaatcgct taaaatcaat aaaggttctt
    16381 ggtaagcatt atcaacctct aatgcttgtt ttaacaataa tacaccttca ttagcatcgc
    16441 cgtgttctat ttccaaacat ccagttgtat acattaactc tttataaaat tgacttagtc
    16501 gtaatccttc tttacccgtt tcaatggcat ctggataatt tttttcattt tcatataatg
    16561 attgtaaata caagtagcct tgaatataat caggatcttt agaaagtaat gtagtcatta
    16621 ttttaattgc ttcttgagtg atgtcatttt tatcgtaaga aatggctttt ttgagataat
    16681 cttctgaagt catttcatct tcattaattt catcgtataa gcgaatcgca tcactatagt
    16741 taccactttg taaactacaa tctgccatac gagagaataa gtttacacca ttaacttgat
    16801 attcaccagt ttctaaaacg gtttcgtatt cagaggtagc acgtaaatat tgaccatcat
    16861 aatataacat ttcagccaat gcaaaatgga ttattggatc atttggctct agttcaagtg
    16921 cttcttgtaa tttatcaata gcaacttcca tcatattaat ttgttgatat aaatctgctt
    16981 ctaacatcaa cttttcaggt gatggttcaa cataacttaa atattctaac gcttcgtcag
    17041 tttgattttc agacattaaa ccttcaataa aataaatcag caattcactt tcgtctggat
    17101 atttgtgata taacacgcgg aatacttcca aaccttgtgg cattaatcca aaattgtaaa
    17161 gtgtctctcc tagaataaat aatgcgtcat cgttgtcagt agttattgct tcattaacac
    17221 gagagtctaa attttctagt ttttgtagat tgatatcgtc tattaattta tagatatctt
    17281 ccattttata ttatccctca ttttctaaaa gctttagttt tggtaaaaat cctggaaatg
    17341 atacatttac agcatcaaat tgtttgattt tgacaggctc gcttgaaagt agagaagcaa
    17401 ctgcaagcat cattcctatt cgatgatcag ttaaactatc aactgttgca tttgttttaa
    17461 attctgacgg atgaataatc aatccatcat tagttggttg taattcaaac cctaacaagt
    17521 ttaacatatc agccgttgta tcaattctat ttgtttcttt tacttttaat tcctcggcat
    17581 ctttaattgt actcgtgcca actgcttgtg tacaaagtaa tgctattaca ggcagttcat
    17641 caattgcttt tggaactaat tctccttcga ttgttattgg ttgaagcatt ggtgtgtatt
    17701 gaatacgaat agaagcagta ggttcagcac cagttgtttg attgaaaagt tggatattac
    17761 cgcccatttt ttcaacaata tcaataatac ctgaacgtgt tggattgatt ccaacattat
    17821 gaattgttac atcacttcct ggtgtgataa gtgctgcaac aataaagaac gctgcagatg
    17881 aaatatcgcc aggaacatga aaatctgcag gtttaatgta tcgaattgct tcaggggttg
    17941 tattaattga taacccttct gcttcaattg gaatattaaa atgtttgaac atcgtctcag
    18001 tatgatttcg acttacatct aattctttaa tgatggtcgg ttccttagaa aacaaacttg
    18061 caaataaaat ggcacttttt acttgtgcac ttgcaacttc catttgataa tttatacctt
    18121 ttatgacaga tggcttaata attaatggtg tataattatc ttcaatacct tcaatattcg
    18181 catccataag tttcaatggt ctcaagacac gatccattgg ccttttacca attgaaacat
    18241 cgccagacaa aacactttca atacctaaac cacttaacaa acctgccaat aatcgtgttg
    18301 tcgtaccaga attacctgta tacaatactt gatgtggcgt gttaaaagat tgatatcctg
    18361 gggaagtcac aactaatttt tcatcatctt ctttgatttc tacacctaac agtcggaaaa
    18421 tgtccatcgt acgacgacaa tcttcgccaa gtagtggctt atatatagta gatacacctt
    18481 cagctagcga cgccaacatg attgcacggt gtgtcattga cttatcgccc ggcacttcta
    18541 tttcgccctt taacggacct gaaatatcaa tgatttgttc atttaccatt tcattcacct
    18601 acttaaaata tgtttttaat tgttcacatg catgttgtaa tgttagttga tcaacatgtt
    18661 gtacaacgat atctccaaat tgtctaatca agaccatttg tacaccttgc ttatcattct
    18721 ttttatcact tagcatatat tggtataacg tttcaaaatc caagtcagtt atcatgtcta
    18781 aaggatagcc gagttgtatt aaatattgaa tataatgatt aatatcatgc ttagaatcaa
    18841 acaaagcatt cgcaactata aattgataga taatgccaac catcactgca tgaccatgag
    18901 gtattttatg atagtattca acagcatgac caaatgtatg acctaaattt aaaaatttac
    18961 gtacaccttg ttctttttca tctgcaataa caatatccag cttcgtttca atacctttag
    19021 caatatattt atccatacca tttaatgact gtaatatctc tctatcttta aagtgctgtt
    19081 cgatatcttg cgtcgctgat tcaccattca ataacgcatg cttataaact tctgcatagc
    19141 cacttaatat ttgctcaaat ggtaacgtct ttaaaaagac taaatcataa atcacagcag
    19201 ttggacgata aaatgcaccg ataaggtttt taccttgctt tgagttaata cccactttac
    19261 cgccaacact agaatcatgc gctagtatag tcgttggcac ttgtataaag tgaacgcctc
    19321 gtaaaagtgt cgccgcaata aacccagcaa aatcaccagt tgcaccacca ccaacagcaa
    19381 taattgctgt attacgagtt acatgatggg ataaaatata ctctaatgtt tcttgatatt
    19441 gctcaaatgt tttcgtcttt tcaccagctg gaataataac tttatgtaca ttttcatatg
    19501 ataaaatatc atcaaattta tcagcaaaat attgatttac atgctcgtca attaatataa
    19561 aactttgatc aaactgatca atatacgtgc taatatggtc aattgcaccg tgttcaacat
    19621 atattggata attatttgaa gggtatgttg tttgtaattt catgattaca cctcaattgt
    19681 tcttgttgtt aaaactcaat atttaattgt ctgcgctcaa taatttgttg tttaagttgc
    19741 tcaatatgat ttgattggaa ttcttccaat aatgcttttg ctatttcaaa tgctacgaca
    19801 tgttcgcaga cgatacttgc tgcaggaaca gcacaactat cagaacgttc aattgttgct
    19861 ttaaagtctt ctttagtatt aatgtctact gaatttaatg gtttatataa cgttggaatt
    19921 ggtttcatta caccattaac gataattggc attccatttg acataccgcc ttctaaacca
    19981 cctaagtgat tagatccacg ataataacca atttcactat tatatagaat ttcatcttga
    20041 atctcactac ctggcttttc agctgcttta aatccttcac caaagcttac acctttaaaa
    20101 gcatttatgc tgacaacacc ttgtgcaatc ttaccatcta acttacgatc ataatgcaca
    20161 taactaccta caccaacagg catattttca actacaactt gaacgacacc gccaattgaa
    20221 tctccttcat ttttagcttc gtcaatttta tctcgcattg cttgtgcgat actgtcatca
    20281 attacacgaa catcattacg atcaagattt gctttaaatg tttctgaatc ataaaaatct
    20341 ttatctttaa ttccacctat ttcaacaaca cgactgtata tatcgatatc taactgttgt
    20401 aataacactt tacataaggc accgactgca actcgagctg ctgtttctct agcagatgat
    20461 cgctctagca catttcgtaa atcacgatga ttatatttca tacctccaac caaatctgca
    20521 tgaccaggtc ttggttttgt aatagtacgt ttcatatttt cacgttcttc ttcacttatt
    20581 ggagctgctc ccataatttt tctccaatgc gtaaagtcat cattggttac aaccatagta
    20641 attggactac ctaatgtata accatttcta acgcctgata ctatttctac tgtatctttc
    20701 tcaatttgca tacgacgtcc acgtccgtaa ccgccttgac gcttaaacat ttctttatta
    20761 atatcctcaa ccttaatttc tatatttgca ggtacacctt caacaataac tgttaattga
    20821 ggtccatgtg attctcctga tgttaggtat ctcatacaca ctcgctcctt tcatacttcc
    20881 acgataacat tataatatgt tgttaatctt atcatattca ttgtgcattc ataaatgttt
    20941 gttttcaagc tcaattaaga aaatggatga catctcattt taaatgtcag aattatctta
    21001 atattaaacg aactaaaatt aattataata tattcgtctg tagaaaagta tcatgaattt
    21061 ctaaacagtt gcatcatata atcattaatt aaaaatgatt agccgtttta atataatcgt
    21121 atcgcaactt tagcaaataa tatactgact acatttctta ataca
//
LOCUS       DALAAR010000047        20178 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000047 DALAAR010000000
VERSION     DALAAR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.049"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(41..733)
                     /locus_tag="O2458_001792"
     CDS             complement(41..733)
                     /locus_tag="O2458_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCZ3887655.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1118..1915
                     /locus_tag="O2458_001793"
     CDS             1118..1915
                     /locus_tag="O2458_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ3887656.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2048..2995
                     /locus_tag="O2458_001794"
     CDS             2048..2995
                     /locus_tag="O2458_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ3887657.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            3597..6551
                     /gene="fdhF"
                     /locus_tag="O2458_001795"
     CDS             3597..6551
                     /gene="fdhF"
                     /locus_tag="O2458_001795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCZ3887658.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6551..7024
                     /locus_tag="O2458_001796"
     CDS             6551..7024
                     /locus_tag="O2458_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCZ3887659.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            7252..7485
                     /locus_tag="O2458_001797"
     CDS             7252..7485
                     /locus_tag="O2458_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887660.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            7507..8283
                     /locus_tag="O2458_001798"
     CDS             7507..8283
                     /locus_tag="O2458_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ3887661.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            8594..9718
                     /locus_tag="O2458_001799"
     CDS             8594..9718
                     /locus_tag="O2458_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCZ3887662.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(9810..10763)
                     /locus_tag="O2458_001800"
     CDS             complement(9810..10763)
                     /locus_tag="O2458_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCZ3887663.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(11125..11625)
                     /locus_tag="O2458_001801"
     CDS             complement(11125..11625)
                     /locus_tag="O2458_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887664.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(11862..12284)
                     /locus_tag="O2458_001802"
     CDS             complement(11862..12284)
                     /locus_tag="O2458_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCZ3887665.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            12532..13614
                     /locus_tag="O2458_001803"
     CDS             12532..13614
                     /locus_tag="O2458_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCZ3887666.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            13709..15109
                     /gene="nhaC"
                     /locus_tag="O2458_001804"
     CDS             13709..15109
                     /gene="nhaC"
                     /locus_tag="O2458_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCZ3887667.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            15283..15525
                     /locus_tag="O2458_001805"
     CDS             15283..15525
                     /locus_tag="O2458_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887668.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(15542..16345)
                     /locus_tag="O2458_001806"
     CDS             complement(15542..16345)
                     /locus_tag="O2458_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887669.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            16955..18910
                     /locus_tag="O2458_001807"
     CDS             16955..18910
                     /locus_tag="O2458_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887670.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            18936..19679
                     /locus_tag="O2458_001808"
     CDS             18936..19679
                     /locus_tag="O2458_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ3887671.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            19743..20069
                     /locus_tag="O2458_001809"
     CDS             19743..20069
                     /locus_tag="O2458_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ3887672.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
BASE COUNT         7123 a         2698 c         3730 g         6627 t
ORIGIN      
        1 caagatattt tctaattgaa aatcatctta ctgctgtttt ttaggaatcg atggggttta
       61 aaaattgatg atagttatca atggatatgt ctctggtttg aatcgtctta ccgaatgaat
      121 acgaaatatc tataatattt tcaatttcga gttcggcagt tttgtcttga gcaatgtcat
      181 aaatgactgc atgccaatgt ccatgtttac acaaaaggcc aataaatata acgttttcag
      241 ctttaatatt atcatcaacc ctatgatcta ctaacatcac atttcgttct gtacaataaa
      301 ttaaaatgtc ggaaaacagc attgggagtg tcatatgttg tgtgtcatca aatttaatat
      361 aaaagtccat gcgtttaagc aattttctta agcgtgtttg tggaagactt aaacattgtt
      421 ttataattgc gttgatttcg cttttatatg gtaatgtcga gtacgactga ctttcattaa
      481 gcactaaaaa taaggctgat aattgtgttt cagataagtt taatgcaatt ttagattgtg
      541 cttgttgtat ttggtaacca ccatttttcc cataatgcgc ataaatttta acaccttgat
      601 tttctaaatc atcaatatct cttaaaattg tgcgtttgga tacgttgcaa tatttagcta
      661 attctaaagc ggtcattttt ttattttgtt gaattgcagt aattattaaa ttttgccttt
      721 cagctttatt catgacagcc acctcctaaa agatgatata ttacacaaag tataacataa
      781 gagtgacaat gaggacattt tcaatatctt ttaaaaaaaa tcgtaaaaat tttgtggaaa
      841 acgcttacat tttaaaaaag attgtgctat gatattactt gtaacagggg gagcgattaa
      901 acaaaggggt agagctatat aacagatagc ttttaatcgt tcaagttaca tcacaacatc
      961 ttcaatattt attacttact ttcctttcta tttgtcggct agcacgtgac tagccgattt
     1021 ttttatgcat aaaatgaaga agtcttcagt tgtattgttg attcttttaa tatagggtaa
     1081 taataaaatg ataatgatat gagaagtggt ggacgaaatg acagataaaa cattacagca
     1141 aatagataag ttaatatgtt cgtggttgaa acaaatagac aatgttattc cgcaattaat
     1201 tatggaaatg actacagaaa ctaagcgtca tcgatttgat ttagttacaa atgtagataa
     1261 acagattcaa caacaattcc aacaattttt agcaacacat tttccggaac accaactatt
     1321 agcggaagaa aaaagtaatg aaatgattac gaatgaaatt aatcatttat ggattatgga
     1381 tcccattgat ggaactgcca atttagtaaa acagcaagaa gattattgta ttatattggc
     1441 gtatttttat gaaggtaaac caatgttatc gtacgtatat gattatccgc ataaaaagct
     1501 ttataaagca atacgaggag aaggtgcttt ttgtaatgga attaagatgg aagaaccacc
     1561 atcgttaaaa ttggaagacg ctattatatc gtttaatgca caagtgatga atctagatac
     1621 ggtgcaagat ttatttgacg cctcatttag ttatcgttta gttggtgctt gcggattaga
     1681 ttctatgcga gtggcaaaag gtcaatttgg ggcacatatt aatacaaatc ctaaaccttg
     1741 ggacattgct gcacagtttt tatttgcaga actattgaat ctaaaaatga cgactttaga
     1801 tggtaaggca attgatcatt taaagggtgc accttttatt ataagtaata aagcatgtca
     1861 tgaaacggta cttaaaattt taaatgcaaa tggtggttat caaaaataca gatagatatt
     1921 ctctagagat aaaaaatgat aaaaaatagt tatacgtcta cagaagtata gtaaaaaaat
     1981 tattttacta tataataaat agagaaaaca gacaaagtat gtaaggaata gtaaaggagt
     2041 ggtgggattg aaacgttcgt ctaaaagtaa aatgagccta ccgaaaaaaa tatttttatg
     2101 ggtattcggt attttagtaa tcttagcgat tgtagctgtt gtgtatgtag ctgctaaaat
     2161 ttttattact ggtaataaga tacacaatcc gttagatcgc aatcattcag aattaagaga
     2221 taaaaaagtg agtttaaacg atggtgatcc attcacaatc gctttatttg gtgtcgattc
     2281 agatgctgat cgtaagaaaa agggtggcgg agagcgtagt gatagtatta tgattttatc
     2341 tatcaaccct aaaacgaaga aaactgaaat agtaagtata ccgcgtgata caagagcgga
     2401 aattgttggc cgtggtacta ctgaaaaaat tgcacatgcc tatgcatatg gtggaccaaa
     2461 tatggctgtt aaatcacttg aaaaattaat gaatgtgcca attgatcatt atgcgacaat
     2521 cgatatggat ggtttgcata atatgataga tagcatcggc ggtgttgatg tagtaagtaa
     2581 tgatacattc actgttgatg gtgttcgttt tacaaaagga cagcagacac atgtaaatgg
     2641 tgatcaagca ttgaagttta ttagaagtcg taaagaagaa ggtgctggtg gtgactttgg
     2701 tcgacaacaa cgtcagcaaa ttgtgcttga agcgatggcc aataagattg caagcccatc
     2761 atcgattaca cattttaata gtctaatgaa tgaaattcaa aataatgtta aaacagattt
     2821 aacattaggt gatttgaata cgattagaag caattataaa gatgctaatg acacgattaa
     2881 taaacatcaa ttgagtggtc aaggcggcat tcaaagtgac ggattgtact acttcattcc
     2941 tagtgagcag tcaaaagctg aaagtactaa gttattaaaa gataatctag agtagtaaac
     3001 taagggaaaa taagttatca aaattgtaca caaattaatt agatagaatg atctgtaaat
     3061 agaataacga atagaactaa tagcccgtaa atagaaggtg ataacacttt gtatttatgg
     3121 gctattttat ttaatgcctg atttgatttt tataaattaa tccattacaa atttaattta
     3181 aagataaata ttaccttcta aatgatagta gatatttatc aaacttgggt tattaatttt
     3241 cagaaaaagg gtatttataa atagtataac gatttttagg tgaggtgatt attaaatatt
     3301 gtcattaata aggttgtgaa agatatggca aaaagcgtga aacagataat tggaaaacta
     3361 ataatgataa ttattttcaa ttaaacaaaa agatgattta atttcgcgca aaatatttca
     3421 aataaaaatg attgtaaaaa ggcaaaatac aaaattttac ttaacaacta gtacataaag
     3481 taatacaatt aaattaattc tatctgaaag atgtgtgggg catcgttatt ttaggtggat
     3541 atgagcaatt tattaaaagt catttacgga aaatatatat agacggggtg agtaatatgc
     3601 aagaacattt ggtggttaca cttgatggaa aagattatct tgtagaacca ggtacgaatt
     3661 tacttgaatt tattaaatca caagatactt ttgtaccttc aatttgttat aacgagtcga
     3721 tgggaccaat tcaaacatgt gatacatgta ctgttgagat tgacggtaaa attgaacgtt
     3781 catgtagtac ggtgattgat cgtccaatga ctgtaaatac tgtgaacaat gatgtgaaag
     3841 atgctcaaaa agaagcgctt gatcgaattt tagaaaagca tatgctgtat tgtacagtat
     3901 gtgattataa taatggtgat tgtgaaattc ataatacgat ggatgcatgg gggcttcagc
     3961 atcaaacgta tgaatataaa gagaagccat acgaaaaaga ttacggccca ttttatcgtt
     4021 atgatccaaa tcaatgtatc ttatgtgggc gttgtgtaga agcatgtcaa gatatcgaag
     4081 tgaatgaaac tattagaatt gattgggatc gtgaacatcc acgtgttatt tgggataatg
     4141 atgtaccgat taatgagtct tcatgtgtat cttgtggtca atgtgcgacg gtatgtccat
     4201 gtaatgccat gatggaagtg aatatggaag gtaatgcggg ttatatgact gatacggaac
     4261 ctggttcatt agcagcaatg attgatttaa ctaaaaaagc agaacctggt tatggtccac
     4321 tatttgcaat ctcagattca gaagcagaaa tgcgtaaaga gcgtattaag aaaactaaaa
     4381 cagtgtgtac ttattgtggt gtgggttgtt catttgaagt ttggactaaa gatagagaaa
     4441 ttttaaaagt acaaccatca catgattctc cagcaaataa aattgcgact tgtgttaaag
     4501 gtaagttttc atggggacat attaattcag atcaacgatt aactaaacca ctagtaagga
     4561 aaaatggtga gttccatgaa gtagaatggg atgaagcttt aaacgtcatt gcagataatt
     4621 ttacagctat taaagaaaag catggcccag atgcactatc attcatttct tcttctaaag
     4681 cgacgaatga agaatcgtat ttaatgcaaa aattagcaag acaagttatt ggcacaaata
     4741 acgttgataa ctgttcaaga tattgccaag cacctgcaac aaaaggctta tttagaacgg
     4801 ttggacacgg cggtgactca ggtagtattg aagatttaga aaaagcggca atgtctgtat
     4861 tgataggtac taatacagcc gaagctcatc cagttatcgc atcacgcatg aaacgtgcac
     4921 aaaaattatt tggtcaaaaa atacatgtat ttgatattag aaaacatgaa atggcagaac
     4981 gtgcggatcg tttttatcaa cctaaaccag gtacggattt agcgtggtta agtgcagtga
     5041 ctaagtatat tattgatcat gatttacacg ataaagcatt tattgatgag tgggtagatg
     5101 attttgatga atattacaaa tcattagaaa catttacaat ggcttttgct gaagaagcaa
     5161 caggtattcc tgaatcagaa ttgattaaat ttgctgaaga atgtgctaaa gctgaatctg
     5221 ttgtaatttg ttgggcaatg ggtattacac aacaagacat tggtagtgac tcaagtacag
     5281 cgatttcaaa cttattatta gtaacaggta attatcgtcg tcctggtact ggtgcgtatc
     5341 cattacgtgg acataataat gttcaaggat gtagtgatat gggaagtatg cctgataaga
     5401 ttactggtta tcaaagtatt gaagcggatg atattcgcgc taaatttgaa aaagaatatg
     5461 gcgttaaatt gaatccaaaa gctggtaaag ataatcatga aatggtagaa ggtatacatg
     5521 acggagaagt acactcattg tacttatatg gtgaagatac gggtattgtg gattcaaata
     5581 ttaattttgt acaagctgcg tttgaaaaat tagatttcat ggtagtccaa gatgaatttt
     5641 taacattcac agcaacatac gcagatgttg tattgccagc aagtccttca cttgaaaaag
     5701 acggtacttt tacaaatacc gaacgtcgta ttcaacgttt ataccaagca ttagaacctc
     5761 ttggtgattc aaaacctgac tggaaaattt tccaagcaat tgctaataga ttaggatttg
     5821 attggaatta caagcatcct agtgaaatta tggatgaagt agcacgctta acacctctat
     5881 atgctggggt aagttatgat cgtttagaag gattcaatag tttacaatgg ccagtacaac
     5941 ctgatggtac tgatgagcct attctatact tagaaggatt caattttgat aatggtaaag
     6001 caaaattatt cccattatca tttgataatt actttaagca agatgaaatt tatgatattc
     6061 atgtaaacaa tggtcgattg ttagagcact tccatgaagg taatatgact tatcaaacac
     6121 caatgattaa atacaaagtg ccacgtgcat ttgttgaaat ttctccagag cttgctgaag
     6181 atagaggcat tcatgaaggt gcagaagtta agctgatttc tgaaacagga gaagcggtat
     6241 tacaagttca cgtgacagat cgtgttaaag gtaaagaaat ctatattcca ttaaataatg
     6301 atgcgatgga aaatggagat ctaggtgcaa ttaacttatt aactaatagt gatgttgatc
     6361 aatacacgga tacaccatct tataaacgta ctagctgtcg tttggaggta attacgaaac
     6421 gtggtaagtc accgttgaac cctaataatt tccgtgtcaa taaaaaacgt caaccgcagt
     6481 acagtgttca agtacagaaa aaatgggaac gttcagatta tgttttcccg ggaaatcagg
     6541 tggataaata atggctgaga gaatatcttc taaaattaga cgcttagaaa aatcagaaga
     6601 acaaattaag ttagaaagtt taaatgaagt tacagaggca attgcagcga ataaagatag
     6661 tatattaaaa gcaatcaagc ttattaaaac attagatgat gcaaaactat tagatgcttt
     6721 aaatggtgca attcgaggac gtcaagtcat cataaataaa tttgcagttg aacttaataa
     6781 agatatttat acagggctat tatctaatat ggcttcaatg gtatttttat taggtgaact
     6841 caatgtatca gatttaagcg acttcctaaa taaagttaat aaaggattac acgttgccaa
     6901 tcaagcgagt ccaaatgcta agacaactat aagaagtcta ttgggcgtat tgaaagatga
     6961 cgacatgaac cgtagtttaa catacatgct taatatgcta aaaggtatgt ctcgagaaga
     7021 ataacattac aatgaaaaag tgtttctaca tgcaagcagt gtaggagcac tttttttaat
     7081 ttttgtaaaa attaaaaagg gtgtttttga tggaataaat gaataatatt gaaaaacaaa
     7141 aaaatcacgt gctattgtga atcaatcatc gacaggccta tgaaacatca tgtattatta
     7201 tgattatgta agttatttaa ataaaaaggg tgagtgcata ttaattcgag aatgttaaaa
     7261 acgtcagctg taatttctaa tacattatta gtcataggtt tagtctgtct atttatgatg
     7321 aaattagtgt tagctattac tttttttgct gtttcattat caattagtct agttgtcttt
     7381 aatatgatgt tcagagatag aacaacgatg aaagtaatcg ttaacgcttc ttttttaatc
     7441 gtaatattag cgattgtagt ggcatatttt ttattgtcta agtaagaaaa atgaggtttt
     7501 gtctatatga agaagaattt caagttacgc atttcaacgc tactattgat agttatttta
     7561 gttgtttttg ctgtattact catcgtgaat gaaactaaat tgtttaaaaa tgatgtgaat
     7621 tactcttttg atgaggctgt ttcaatgcaa caagggaaag gtattgtaca gacaaaagaa
     7681 gaggatggta aatttgttga agcaaataat aatgaaattg ctaaagcaat gactatttca
     7741 cataaagaca atgatatgaa gtatatggat atcacagaaa aagtgccaat gtcggaatct
     7801 gaagttaacc aattgctaaa aggtaagggg attttagaaa atcgagggaa agtttttcta
     7861 gaagctcaag aaaaatatga ggttaatgtc atttatcttg ttagccatgc attagtagaa
     7921 acaggtaacg gcaaatcaga attagcaaaa ggcattaaag atgggaaaaa acgctattac
     7981 aacttttttg gtataggagc attcgatagt agtgctgttc gtagtgggaa aagttatgct
     8041 gaaaaggaac aatggacatc accagataag gcgattattg gtggtgcaaa gttcattcgt
     8101 aatgaatatt ttgaaaacaa tcaactgaat ttatatcaaa tgcgatggaa tccagaaaat
     8161 cctgcgcaac atcaatatgc gagtgacatt cgctgggcag ataaaattgc caaattaatg
     8221 gataaatcct ataagcagtt tggtataaag aaagatgata ttagacaaac atattataaa
     8281 taagacatcg gtgcttaaag gagctggaac aatttattgt ttcgagctcc tttagcgcat
     8341 tctgagtgtg ttagttaaat ggattttaac ctaacaaaaa acgctatata gcatcaaata
     8401 tgctatatcc cacatcattg ttacaaatgt acatgatgta aatgaatatt gctgtctaaa
     8461 tgtgcatgta atatacaatg gtgcagataa tacacttaag tccttaaaaa tgaaacgtta
     8521 gttccaagag tcatttttaa acaatagtgc atgtgataaa atagaaaaga atgaaaaata
     8581 tagaggtgac aatatgaaga tagcaattat aggtgcaggc atcggtggat taacagctgc
     8641 tgcattatta caagaacaag gtcatactat taaagtcttt gaaaaaaatg agtcagttaa
     8701 agaaattggc gctgggattg gtatcggaga taatgtgctt aaaaaactag gtaatcatga
     8761 cttagctaaa ggtattaaaa atgctgggca aatcttatct acaatgacag tgttagatga
     8821 caaagatcgc ctgttaacta ctgttaaatt aaaaagtaat acattgaatg tgacgttacc
     8881 acgccaaaca ttaattgaca ttattaaatc ttatgtaaaa gatgacgcaa tatttacaaa
     8941 tcatgaagtc acgcatatag ataatgagac agataaagtt accatacatt tcgcggaaca
     9001 agaaagtgaa gcatttgatt tatgtattgg tgctgatgga attcattcta aagtgagaca
     9061 atctgtaaat gctgacagta aagtattata tcaagggtat acatgcttta gaggtttaat
     9121 tgatgatatt gatttaaagc atccagattg tgcaaaagaa tactggggaa gaaaaggaag
     9181 agtaggtatt gttccgttat taaataatca agcatattgg ttcattacaa ttaactcgaa
     9241 ggaaaacaat cataaatata gttcgtttgg taaacctcat ttgcaagcat actttaatca
     9301 ctatccaaat gaagttagag agatcttaga caaacaaagt gaaacaggta tcttattgca
     9361 taatatttat gatttgaaac cactcaaatc ttttgtttat ggtcgtacta ttttactagg
     9421 agatgcagca catgcgacaa cgcctaatat ggggcaaggt gctggacaag caatggaaga
     9481 tgctatcgta ttagtaaatt gttttaacgc atacgacttt gaaaaagcat tacagcgtta
     9541 tgataaaata cgtgtcaaac atactgcaaa agtaattaag cgttctagaa aaatcggtaa
     9601 aattgcccaa tatcgtagtc gtttatttgt tgcagttaga aatcgtatta tgaaaatgat
     9661 gccaaatgca ttagcagctg gacaaactaa attcttatat aaatcgaaag aaaaataata
     9721 caacaatatg aaaacccccg tatgttgaaa cgagagctca acatatgggg gttcttgttt
     9781 ttataatgtt attataataa attcaattat tagttaacga caaattgtgg tttctcacct
     9841 tgaacggcac taattgcagc attagcaaca attttagaca tcatgtcacg tgcttcaaat
     9901 gtagcattac caatatgcgg tgttaatact acattattaa gtgattttaa gtcatcggta
     9961 atatctggtt caaattcata tacatcaagt gcagcacctt caatttcatt atctttcaat
    10021 gcttgcacta gtgcttgttc gtgcacgatt ggaccacgag aggcattgat taaatacgcc
    10081 gtagatttca tcattttaaa ttgttctgta tcaattaaat gatgcatttt aggattataa
    10141 gcagcgttga tagtgataaa atctgcattc tttaatagtg tatctaaatc tacatatttt
    10201 gcaccgattt ctcgttcttt ttcttctttg cgattaggtc cagtgtatag cacatccatg
    10261 tcaaatgctc ttgcacgacg agctactgca ctaccaattt cacctaaacc gataatgccg
    10321 attgttttcc cagatacttc tctacctctg aaaaataaag gtgcccatcc atcaaatcca
    10381 gttgtacgtg ataattggtc cccttcaaca atacgacgcg ctactgcaag tactaatcca
    10441 attgttaaat cagcagtcgc gtttgttgat gctttaggtg tgtttgtaac atctatactt
    10501 ttttctcggg catactcgat atcaatatta ttaaaaccag cgccatagtt ggcaatgatt
    10561 tttaagtctt taccagcatc gataacatct ttatcaacgt ttgtagataa taaactaatt
    10621 aaggcagtcg cgtttttaac acctttaatt aaagtgtctt tatcgactaa tcctttacct
    10681 tcatacattt caacttcaaa atgttcttgt aaaagtttta aacctacttc tggtattgca
    10741 ccagcaacat aaactttttc cataaaagat cactcctttt atcttagtat agtagaagat
    10801 tagacagtat acaactatgt catgatgtct tgtgtatcaa tgatgtaagc gcgtactttt
    10861 gatggaggtg atataactta ggcactgtag aactatgaat attgtaatgt ggaaaaactg
    10921 gatcaattaa attagataac gtagttttaa agttaatagt attagaaaaa attaatattt
    10981 tgaatatggg aggaaatata aataagtagg tggcaacgaa aaatagcaaa aaaagagctt
    11041 ctcctataaa ggaaagctca aagttttttg atgacatatg tactagaatt aagtttcaag
    11101 acaatatgta tcatcgtgtt tatattaaat atggatgtag ttgtagttac ctgcttcact
    11161 tgcagaaata gttctagaac ttactgagaa aggtccgcca ctataattca tttctgaaat
    11221 tgtaactgaa ccatcactgt ttacactttc tacatatgca acgtgaccaa atggtccttc
    11281 agaagattgt aggatagcgc ctttagaagg tgtatgattt actgtgaatc cagcaccttg
    11341 tgcagcagca gcccaattat tagcatttcc ccaagtagaa ccgatttctc cgccaacttt
    11401 atcatataca taccaagtac attgtcctgc agtgtataag ttaccagaat gtgaaattga
    11461 tgatgtagtt gtcgtagttg tcgtagtcgt tgtagtttga gtcgtgttgt agttatagtt
    11521 gttgtaattt gtataatttt cagcagcatc tgcatgatgt gcttgaccta ctaatgctgt
    11581 gccgattcct gctgttaacg tagttgctgt tactaatttt ttcatgaata aagtcctcca
    11641 aagttctata tcttttttta taaataaaac gtagcgactg ttttattctc acatctcgaa
    11701 ttgatgacaa tagttacttt aacaaaatta atgcttcttg tggggaatgt tattgatttg
    11761 taaaagaata aaaaaacttt gactaatttt gtaataaaaa ttagtcaaag ttacaatgag
    11821 attaacagat aattaatagg aaatatttat ttgtaatatg tttaaataaa tcgaattgtt
    11881 aaaggtatta tatattcttg gccattataa tatttgacac acgcaataat tgtgaataca
    11941 aaagataata ttgagaaagc gaatatggat aaaataccga taaacgtaat gatgaaacct
    12001 ataataataa tgaaatcaat atctgtagca attaggaaaa cgcctattaa agtgataacg
    12061 actaaaacga tagaccaaat aatataagaa atcgtatagt taagataatt ttttccagca
    12121 cgatcaacta gtttcgattc atcttttttc aataaccata ttatcagtgg accaataata
    12181 gatgtgaata aacttaataa atagataagc atcgccataa tgttctcatc attggatttg
    12241 cgattcggtt gatgatttgt tacgtcgttc atttcagttg tcatattaga cactcctttg
    12301 aaaattgtaa tattatcttt aactataaca aaatataatc aaaaataaac atgtttatta
    12361 aacaattatt aaaaataaaa ataattggtg gacgtcggcg tttaaatagg ttaatttaag
    12421 gttatatata cttaacattt ataatgatgc gtaatgaatt cgcatcattt ttatattgtc
    12481 ttacgtataa tttgttttta attttaacca aagatagaaa gagggttgtt tatgaaaata
    12541 gcaattgtag gatcaggaaa tggcgcagtt acggcagcag tagatatggt gagcaaaggc
    12601 cacgatgtta aattatattg tcgtaatcaa tctataagta agtttcaaaa cgcaatcgaa
    12661 aagggcggat ttgattttaa taatgaaggt gatgaacgtt tcgtaaaatt cactgatatt
    12721 agtgatgata tggaatatgt tttaaaagat gctgaaattg ttcaagtgat tattccatct
    12781 tcatacatag agtattatgc tgatgtaatg gcagagcatg taactgataa tcagttgata
    12841 ttcttcaaca tggctgcagc aatggggtca attcgtttta tgaatgtttt agaagataga
    12901 catattgaaa caaaaccaca actagcggaa gctaatacgt tgacgtatgg tacgcgtgtc
    12961 gattttgaaa atgcagcagt tgatttatct ctaaatgtac gtcgtatctt cttttcaaca
    13021 tatgatagaa gctgtctaaa tgattgttat gacaaagttt caagtattta tgatcattta
    13081 gtaaaagagg aaagcttaat taaaacaaat cttgaaaatg gtaatcctga agtgcatcca
    13141 ggaccaacat tattgaatgt cggtcgcatt gattatgctg gcgagttcgc tttatataaa
    13201 gaaggaatta ctaaacatac agttagatta cttcatgcaa tcgaattaga acgtttgaat
    13261 ttaggccgta gattaggttt tgaattatca acagctaaag aatcacgtat tgaacgtggt
    13321 tatttagaac gtgataaaga agatgaacca ttaaatcgtt tgtttaatac aagcccagta
    13381 ttttcacaaa ttccaggacc aaatcatgta gaaagcagat atttaactga agatattgca
    13441 tatggtttag tactatggtc aagcttaggt cgtgttattg atgtaccgac accaaatata
    13501 gatgcagtaa ttgtaattgc atcaaccatt ttagagagag acttctttga ggaaggctta
    13561 acagttgaag aaattggttt agataagctt gatttagaaa aatatttaaa ataaatgatg
    13621 gcttgaagat agaaaaggat atagcattat gcaaaagcaa taaattgaag aaaagaggtt
    13681 tctcatcaat aagcgaaggg gacgatagat gatgaaaaga aaacccacct ttttagaatc
    13741 aatttcgaca atgattgtaa tggttattgt tgttgtaaca ggctttgtgt tttttgatat
    13801 tccaattcaa gtattattaa ttattgcctc agcatatgcc acatggattg caaaacgtgt
    13861 aggcttaaca tggcaagatt tagaaaaagg cattgcagaa cgtttaaata ctgcaatgcc
    13921 tgcaatttta attatactag cggtaggaat tatagtaggc agttggatgt tttctggcac
    13981 agtgccagcc ttgatttatt atggcttaga tttattgaat ccaagctatt ttttaatatc
    14041 agcctttttt ataagtgctg ttacatctgt agcaactggt acagcatggg gctctgcatc
    14101 aactgcaggg attgcactta tttctattgg taatcaattg gggattcctc cagggatggc
    14161 agcgggtgct attatagcag gggctgtgtt tggcgataaa atgtcaccat tatcagatac
    14221 aactaattta gcggcgcttg ttactaaagt taatatattt aaacatatac attcgatgat
    14281 gtggacgacg atacctgcat caatcatagg tttattagta tggtttattg ctggatttca
    14341 atttaaaggg cattcaaatg ataaacagat tcaaactttg ttatcagagc ttgcacagat
    14401 ttatcaaatt aacatatggg tctgggttcc cttaattgtg atcattgttt gtttgctatt
    14461 taaaatggct acagtgccag ctatgctaat atcaagcttt tctgccatta tagtggggac
    14521 ttttaatcat catttcaaaa tgacagatgg tttcaaagca acatttagtg gttttaacga
    14581 atcaatgata catcagtctc atatttcatc cagtgtgaaa agcttgttag aacagggtgg
    14641 tatgatgagt atgacccaaa tattagtaac gatattttgc ggatatgcat ttgcaggtat
    14701 tgtagaaaaa gcaggatgtt tagaagtctt attaactact atttctaaag gcatccattc
    14761 tgtaggaagt ttaatatgta ttactgttat ttgttgtatt gcgcttgtat tcgctgcagg
    14821 tgttgcttcg attgtaatta ttatggtcgg tgtgttaatg aaagatttgt tcgaaaaata
    14881 ccaagtctct cgttccgttt tatcaagaac acttgaagac tcaagtacga tggtcttacc
    14941 tttaatacca tggggaacat caggtattta ctatacgaat caacttcatg tctctgttga
    15001 agaatttttc atatggacag taccatgtta tttatgcgca attatagcaa ttatctatgg
    15061 ttttacaggg ataggtatta aaaagtcatc gaattcacgt ttaacttaat gtgagcgtgg
    15121 aatatatata atatgttgaa acactttaat catttataat tgtagcggtt ataatttgaa
    15181 aaggttttaa cttagaataa atatcctcta tacatatact gaatatgttt tgtagcggaa
    15241 catgttgata tatgtaatgt aagttttatg tcatgatttg taatgactaa attaattgag
    15301 aatttgaagg caagtatatt tgtaagtact ttaactaaaa atttatcaat gtatagccga
    15361 tttgacatgc ctaaatttgg gtgtgtcaat ggctgtatgt tgtttattct ttattacaga
    15421 gtgaatcgga ttggtgaaaa tcgaaatttt gagattttta ccaattcgat ttttttcata
    15481 gaaattaaaa aagccaacaa ggctcttgaa accttgttgg cgtaaacata gccatcacta
    15541 attagtgaat gaagttataa ccagcagctt ggctagctga gattgtacgt gaagttacaa
    15601 cacctgggcc ataaccatag ttcatttctg aaactcttac tgaaccattg ctgttaacac
    15661 tttcaacgta tgcaacgtga ccgtatgcac cttgagttgt ttgcataatt gcaccagctt
    15721 ttggtgtatt gttcactgtg taaccagctc ttgcagctgc gttagcccag ttacttgcat
    15781 tgccccaagt tgaaccgatt ttaccaccta cacgatcaaa tacgtagtat gtacattgac
    15841 cagaagtgta taagttacgt cctgaagtat aaccacttga gattgaacgg ccatttgatg
    15901 atggagccat agttgtagtt acttgaacat tgttgcttga agtgctgtag cttgcaccta
    15961 aaccaccagt acggtagctg tttgtgttgt aactattata gttattgtag ttatatgatt
    16021 gattattatt tgagtagttg ttgtaacggc tgtagttatt gtagctataa ccgttgttgt
    16081 aattgttata gttattgtaa ccattgtagt agtaatagct gtagtagcca ttatcttggt
    16141 ttaattgact tggatgccag ttacctttcc atgtgtaatg gtagttacct tgtgcatcaa
    16201 tagtgtaagt atagctatat gatgttgggt cgtttggatt ataaccgtag ttatcttgct
    16261 cagaagcatg agcttgattt cctgatgcaa ttgcgattgt agcgaatcct gcagttgcga
    16321 tagtagctgt agcgattttc ttcattttaa aaatatcctc ctaaaaattt taaatctaaa
    16381 atattttcgt aatgtccgtg tgacaaaatt aatgttataa gttatctctc gtaattaaac
    16441 gacaagaaag actataacag aaattagcgt ccttgtgtgc tttgttaacg ttttgtaatt
    16501 ttttgctaat atcttgacac aatagaattt taaaagtata gaaatttgca ttttgcaaaa
    16561 cttataacta cggcattctt tgtgaaaact gaatgtttcg aaaataagtc tgttacaaat
    16621 ttgtaatatt actgaaaatt ctaaatgtat attttgtgca taatatagga cttttaatca
    16681 gaaaatgcat aaaaattcga cacgaacgat caagtttcta tgattatgaa agctttggta
    16741 tttggatgaa aatgtatatt taatggataa aatatcctaa tttagcataa aaaaatgttt
    16801 taataaaagt attatttgat ataatcgatt tatgttttgt tactgctaaa aaacatgtgg
    16861 cgatataatt ggtagtgaat attagatgtt gacaattgat ttgagaaaga caaacaggag
    16921 taaatcaata tatttgtata tgtgaggatg taagatgaca gataattatt cactacatat
    16981 ttataaaagt atgaaaatga gtagtgtgaa ggaagcgaac ttaaaaatta tatattggtt
    17041 acaaggtaca ggtgtagttt caatcaatct acaacaatat gatctaaaaa gaaattatgt
    17101 caccattatt atgttgaatg atttgtatca aattgatggt aatgatgata gtgtatgttg
    17161 tgttgttgaa gtaagtgcaa agacattttt gaagtttatg aatgcaaact atatgatgcg
    17221 tggcaagata ttaacaaatg aggttgctag cacttttaga atcttgatta agtatttaat
    17281 ctacagcaaa attaaacaac agtcttataa tgctaatgat aagattatta attacatgtg
    17341 tgttgaatta atgtcattaa atacatacga tggtagtcgt catttagtag ccgaagaagt
    17401 gcacgattat ttaacaaaca accaccataa aaagataaat aggaaagatg tcattaatca
    17461 agttagtatt acaaacaaag ctttaagtga catgtttaaa gcgacacctt atagtaattt
    17521 tattcaatat ttaaatcata ttagactgga gcattgctta attgatattt taacttctaa
    17581 gaagcctatt gaagaaatcg caagtagtca tggttttaat cattattctc gttttataca
    17641 cctctttaaa gaaacgtatg gtaacactcc taaattaatt agaaaaagat atagtccaac
    17701 atctcattca attaaccatt cccaattagt tgaaattgat aaaaatataa ttgaattatt
    17761 tgatgatact aatcaaagtt cttcaagtat gcgcgagatt gatatacctt ttgagcctac
    17821 aaaaaggagt caaatgtatc aacctaaaaa catttatatt aaaggaagta atttttattg
    17881 gatggattat tatactatta aacaaatcat tcaaagatta ggtgttaatc ctgaaaactt
    17941 gcatataatt gttacaatcg acacaagcaa tgagctatcg attgaacaaa tcactttatt
    18001 acttcaaaag atagtgaatt acaatgtaaa tgtagtgttt cgtattaaac atgaatataa
    18061 ggaattgtta acgagtgctg aaagaggcaa gatagaaaag ctagtagcta caatttttaa
    18121 tatgactttt gagagtaata gatgtaagat tgcattttta attaacgact taaatactac
    18181 tgcaattcaa aaactgaaac gtttaataga taattatata ggagtatttg aattaatata
    18241 tcatctggaa ccaaaagagt tgaatacgcg ctatgataaa gaaattgatc atctaataga
    18301 ctattttatc ttaccgctaa gacaaataga acaggtgtct attgcacgtt ctaaaattat
    18361 atgtgatgta gatttgatta atgagaataa tgacttaaaa gaaaaaatca caatgtctga
    18421 tattaataag gtttctgaac ttttgacaaa ttatacaaaa tttcgaggac ttattatgca
    18481 catgtcaatt ataagtgaat cgagcaaaga aataaattta actagattat caatattcat
    18541 ttttatcata aatatactta atcaattacg agggattgtt atttatcaaa atgacacgat
    18601 gattgtaacg aagttcaaac atgaagtaca atgtgtcatt tgtttgccag ccgcattgtt
    18661 aaatgagaat tcagacgcta tacgtttatc gtgtaagggt cttcaacaat ttagccatgc
    18721 taaaactaaa caaattgttt taagtataga taaagataat catacttccg aaaatggtgt
    18781 acttgtgaat gatattatgc aacaacacag ttattggaaa gctaatttta tagaaagaaa
    18841 tatttatgat cattcattaa tggtctcaag caatagtatt gttcatttga aatatcatct
    18901 tgagatatag gcatggttta agagaggttt taaagatgtc taaatttaaa gttaagcgtt
    18961 taactgcaca catactaatg ttgaaagtca ttaatgatat gttgaaatat tcatttcatc
    19021 tcactttgac tcaagtgaaa ctattaaatc tattagttaa atatgataat tctgattcca
    19081 atagcgcttc aatagatagt ttattaaaaa tgaaagaaat ccaatctaag atggcccttc
    19141 ttcaaatgtt aagttattta aacaatcatc aatggctatt gaaaggaaga gatcaaaggg
    19201 atcaacgtaa attgactatt tcgattaata aaatgcatat agataaaata gaatatatga
    19261 ataatgagct tagtgactat attgaacgtt attttggtca atatacattt gattttagtt
    19321 gtgactatgt atcgtattta ttgtcgtcac aagaattatt gcggaatcta aagtgttatt
    19381 taaatatgtg tcaattatca ttagaagagt tatacgtttt aggaatatta aatctgcata
    19441 aaggtcaatt gacggttaaa gaattacagg gtgaattcca tcacccgata tttgcggtta
    19501 gcccgatttt aaagtgttta attttgaagg gattagttaa aaaagagaga tgtgaattag
    19561 atgaaaggcg tgttattgta acaatcaagc gagaaaagtt ttctaaagtc actatgctta
    19621 ttcaagcatg ttataattat cttgaaaagg gaatacaagt taatcttaat aataaatagt
    19681 taatcacttg tatttcatat acctatgaag aatagtttat agttttaagt gggagggata
    19741 ttatgatttt agataatgtg aatccaaatg atttatttcc aactgaaaaa aagggtccgt
    19801 cagtgctagg aataattgag tatcaagttc aaggtgagaa tgaatttgaa ggggcattta
    19861 tagcaacaaa tgaaagatta attatgaatg tagatatgaa tggccaattt tattatagaa
    19921 gtattagtta taatgaaata gagaagatag attatgatgg tcaaactata atgttcaaat
    19981 ttaatattgg taatgtccca atgcatgata ttaaaagtgc taacgttgaa atgtttgttg
    20041 aatatgtaaa acaacatatg atagtataat gtcaatgtat gtaggttgat aagaagagtt
    20101 tgaatgataa ctcattgcat ttaaagataa atggcatgcg ttatgattta aagctcttct
    20161 ttttttatat tttataaa
//
LOCUS       DALAAR010000048        19711 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000048 DALAAR010000000
VERSION     DALAAR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.050"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(138..2114)
                     /gene="sfaD"
                     /locus_tag="O2458_001810"
                     /gene_synonym="sfnaD"
     CDS             complement(138..2114)
                     /gene="sfaD"
                     /locus_tag="O2458_001810"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCZ3887673.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2216..3409
                     /gene="sfaA"
                     /locus_tag="O2458_001811"
     CDS             2216..3409
                     /gene="sfaA"
                     /locus_tag="O2458_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCZ3887674.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3396..5153
                     /gene="sfaB"
                     /locus_tag="O2458_001812"
     CDS             3396..5153
                     /gene="sfaB"
                     /locus_tag="O2458_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCZ3887675.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5160..6230
                     /gene="sfaC"
                     /locus_tag="O2458_001813"
     CDS             5160..6230
                     /gene="sfaC"
                     /locus_tag="O2458_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCZ3887676.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6331..6456)
                     /locus_tag="O2458_001814"
     CDS             complement(6331..6456)
                     /locus_tag="O2458_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887677.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6622..7605
                     /locus_tag="O2458_001815"
     CDS             6622..7605
                     /locus_tag="O2458_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3887678.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7617..8648
                     /locus_tag="O2458_001816"
     CDS             7617..8648
                     /locus_tag="O2458_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ3887679.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8645..9613
                     /locus_tag="O2458_001817"
     CDS             8645..9613
                     /locus_tag="O2458_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ3887680.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9849..11219
                     /locus_tag="O2458_001818"
     CDS             9849..11219
                     /locus_tag="O2458_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ3887681.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11351..11611)
                     /locus_tag="O2458_001819"
     CDS             complement(11351..11611)
                     /locus_tag="O2458_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887682.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11788..12303
                     /locus_tag="O2458_001820"
     CDS             11788..12303
                     /locus_tag="O2458_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ3887683.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12347..13534
                     /locus_tag="O2458_001821"
     CDS             12347..13534
                     /locus_tag="O2458_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCZ3887684.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13553..14236
                     /locus_tag="O2458_001822"
     CDS             13553..14236
                     /locus_tag="O2458_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCZ3887685.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14413..15756
                     /locus_tag="O2458_001823"
     CDS             14413..15756
                     /locus_tag="O2458_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887686.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            15855..16328
                     /gene="sepA"
                     /locus_tag="O2458_001824"
     CDS             15855..16328
                     /gene="sepA"
                     /locus_tag="O2458_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCZ3887687.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            16650..18092
                     /gene="lmrS"
                     /locus_tag="O2458_001825"
     CDS             16650..18092
                     /gene="lmrS"
                     /locus_tag="O2458_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCZ3887688.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18240..19304
                     /locus_tag="O2458_001826"
     CDS             18240..19304
                     /locus_tag="O2458_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCZ3887689.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19393..19578
                     /locus_tag="O2458_001827"
     CDS             19393..19578
                     /locus_tag="O2458_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887690.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
BASE COUNT         6526 a         2681 c         3616 g         6888 t
ORIGIN      
        1 gggatttatg tcccagcctg ttttttgtga tttttaataa tttgaatatg gaaaatgtat
       61 tattctctca tttgtataga ttgtatttaa taagttaatg taatccttga gctcacgatt
      121 aataaaattc tataacctta attattttct cgatacaaag ggttattaac tttaatataa
      181 gtataatgat gtgcctcatc ttcaagacgc attgttgtaa cacacttata atctataaat
      241 ggcgcgaaca tggtatcttt aatttcattt atgcgattct catttacttg attagatttg
      301 tgtgtcgaaa gtacaagttg atcaaaaatg ttatctagta catcacgaac gatataccac
      361 atatgtcttt ctaagtttga atcatttgat gctttagaga ttgtaagaat taattcgcct
      421 aaatggtttt gaacggtaga ataaaaggct ttgttaaaca cagacgtttt tgaatcagta
      481 agaattcttg atttttcatg gaaatgagat gtactgtatc ccatttcatt tagttgtgct
      541 ttatcaattc ttaaaccttc aaagtctcta atatacatcg tgtccaatag accatctttc
      601 cgaaatgttg caatagcatt ttgcaagtga gcctctaatg caatgccata tttagtaact
      661 agtgggatta cgagaccaag caatgcttta ctataagttt caacccacga tttcgccgat
      721 gattcaaaat cagacaatga tgcagctgat tgataacgtt taatcaatgt cacgataggt
      781 gattcattgt taaatgggta ggttgcaact aagcttgaag gaatcaatgg tgtgacttct
      841 tgtggaatca tttggtatat attttttcta aataatgtac ctaattgttc acttcgttca
      901 gtttgatagt ctgcttcgtc ttgctcatta taaaaatgaa taccagcaac ttcatcaata
      961 attgttgatg cataggactt aaatatgaca tctttctcca aaatatcatt taaaatacgt
     1021 gtcattagtg gaccattgtg cgtcgtttgt tctgataatg tacgaatctc acctgtaata
     1081 tgaacgtttg tcgacaattt gatgtgtggc gacatagctg ggtatttagg aactaatgtt
     1141 ctgaaagata aaccagcata ataatccaac gtatgttttg cttcaatgat taattcttta
     1201 tctacttctg cttgataatc agaatgtaat acgtcatcta attgccatgg atgaacaatc
     1261 ataatgtgat aatcattaaa gttaaacttt ggcgtaaatt cattttctaa ttgtttaatt
     1321 aagtccggaa atagttgatg aacagttgta tcataatctt tagacagtga catagtacgg
     1381 cttaatttac tgtgaatcag tactattttc aacttaatag gttgattgaa ttctgaagaa
     1441 tataggaatg tttgtaatgc atttaaacct ttacgtaatt tagccccagg atgtagcgga
     1501 tgaccttcaa taacggcttg ctctgaacgc aagtaactat cttcgctatt ttcgataata
     1561 ttaaataaag gtgcagaatc atgttgcatt gacagtgctt gatagctaat tgcaaatgtc
     1621 atattagttg cactgtttat taaatcttgc tgaaattgat cactagcagc attttttaaa
     1681 tctggtgctt caattaaaat acactcaaga atttcatttg gatggtgtac tcgtgtaatc
     1741 gtatttgtaa tgtcatcttt aatgtagaaa gggccttcaa catcaattcg atcaaaggcg
     1801 tgttctccag tgataggagc atataatgtt tgcttagctt gtgggaactg gatttctagt
     1861 atatgagtcg ttgagatatc taacataatc aaatcacgac tcaatatttt cttactttga
     1921 gtgcgcgctt taactaagtt ttcgcgatgc attgatgtga ccaatctctg agtgacttta
     1981 tctcttcctt gtaaaatcat ctctttaaaa atattagccc aatcgctatt atgttgttgt
     2041 aaaaataaat atgtttcttg ttcttctttg ttaaatttta atgtctgttc tttaaaaatt
     2101 aagttcaagt tcataattca cctctatgaa atattttaca aaagcaagat agatttgtat
     2161 aatccatatt aatgataatg attcttatta tcaacagaat gcgggtgtaa gttttatgac
     2221 aaaatatttt tttagcagtt cttttctact atttctaggt aattggattg gacaaatagg
     2281 gctaaattgg tttgtactta ccacttatca taacgcagtt tatctgggga ttgtcaattt
     2341 ttgcagactt gtaccaatat tattactaag tgtgtgggca ggggcaattg ccgataaata
     2401 tgataaaggg cgattgctga gaattacaat ttcatcatca tttttagtaa ctgcaatttt
     2461 atgtgtgctc acgtatagtt tcactgcaat tccaattagc gtcattatta tatatgcgac
     2521 attaagaggg attttaagtg cggttgaaac acctttaaga caagcaatct taccagattt
     2581 atcagataaa atatctacta cacaagctgt atcatttcat tcattcatca ttaatatttg
     2641 tcgttcaata gggcctgcca ttgctggtgt catattagca gtctatcatg cgccaacaac
     2701 atttcttgca caggcaattt gttattttat cgcagtttta ttatgcttac cattacattt
     2761 taaagtaact aaaatacctg aagatgcatc aagatacatg ccgttaaaag ttattataga
     2821 ttacttcaaa ttacatatgg aaggtcgaca aatatttata acatcattat tgattatggc
     2881 gacaggtttt tcatatacga cacttttacc agttttgaca aacaaagtat ttccggggaa
     2941 atctgaaata tttggtatcg ctatgacgat gtgtgccatt ggtggtatta ttgcaacgct
     3001 agttttacct aaagtactta aatatattgg tatggtaaat atgtattatt taagttcatt
     3061 tttatttggc attgctttgt taggtgtggt atttcacaat attgtcatca tgttcatttg
     3121 tattacattg attgggttat ttagtcaatg ggcacgtacg acaaatcgcg tttattttca
     3181 aaataatgtt aaagattatg aacgtggtaa agtactgagt attattatga tggatagagg
     3241 tatgattcca ttgggaagtc tattaatgag tatatgtgca gatgtgtttg gcattgttag
     3301 aactttttca ataatgggaa taagtactat atgcattaca atggtattct attttataaa
     3361 tagaaagttg aagttaaagt tggaggaaag taatcatggt atatcttgaa tgggcaaagg
     3421 cagatagaaa tattcaatat cgtgtaatta acgccattat taaagaacgt atttaccccg
     3481 agcaaacatt tatttcgcaa aaaggatctt taattgaaat tcagtatcat atgcatgtgt
     3541 tgactattga agttgttaga aaaagtgcat tagaacgcta tgagtttaca ggtgatatta
     3601 cttatttaaa taaaggtgaa acgtcattaa ttataacttt agaaggttta ttagatgtgt
     3661 tgaatcatga ctttgatatc cctatttcag agcgactacg cgaagagtta atacacagtc
     3721 gagatagttt agttgaaaca tataagcaaa tgtctcacag acaaacgtta ataagtcaaa
     3781 gttttaaatt ttcaaggtta ccacaagata ttaacttttt ttcatggtta caacatgtaa
     3841 aagatagtga taagacagat gatttaactt attctgagag tttggtacca gaggggcatc
     3901 caacacaccc tttaaccaaa acgaaattgc ccttaactat ggaagaagta cgagcatatg
     3961 cacctgagtt tgaaaaagaa atccctttgc aaattatgat gattgaaaaa gaccatgttg
     4021 tgtgcacagc tatggatggt aatgatcaat ttattattga tgaaataatt cccgaatact
     4081 acaatcagat tcgtgtgttt ttaaagagtt taggtttgaa aagtgaagac tatagagcga
     4141 ttttagtaca tccttggcaa tatgatcata cgatagggaa atattttgaa gcatggattg
     4201 ctaaaaaaat attaattcca acgccgttta caatactttc aaaagcaact ttatcattta
     4261 ggacgatgtc tttaattgat aaaccatacc atgttaagtt gcccgtcgat gcacaagcaa
     4321 caagtgccgt tagaacagtc tcaactgtga ctactgtaga tggaccaaag ttaagttatg
     4381 ctttacaaaa catgttgaat caatatccag gatttaaagt tgctatggaa ccgttcggtg
     4441 aatatgcaaa tgttgataaa gatagggcac gtcagcttgc ttgtattata agacaaaagc
     4501 ctgaaattga tggaaaaggt gcaacagtag ttagtgcaag tctagttaat aaaaatccaa
     4561 tagatcaaaa agttatcgtg gatagttact tagagtggtt aaatcaagga attactaaag
     4621 aaagtattac gacatttatt gaacgatacg ctcaagcatt aatcccgcct ttaattgctt
     4681 ttattcaaaa ttatggaatt gctttagaag cacacatgca aaatacagta gtgaacttgg
     4741 ggccacattt tgacattcaa tttttagtga gagatttagg tggttctaga attgatttag
     4801 aaacattaca acatcgtgta tcagatatta aaattacaaa tgatagttta atagctgatt
     4861 ctatagatgc agtgattgca aaattccaac atgctgttat tcaaaatcaa atggcagaat
     4921 taatccatca ttttaatcag tatgattgtg ttgaagaaac cgaattattt aacatagtac
     4981 agcaagtagt agcgcatgcc attaacccaa cactaccaca tgcaaatgag ttaaaagata
     5041 ttttgtttgg accaacaatt actgtcaaag cgttgttaaa tatgagaatg gaaaataaag
     5101 taaagcaata tttaaatatt gagttagata atccgataaa aaaagaggtg tagtactaca
     5161 tggcacacgt taacataaat atatcgaaga ttaaatataa cgccaaagta cttcaaacag
     5221 tttttcaaag taaaaatatg caattcacac cagtaattaa gtgcatagct ggtgaccgta
     5281 caattgtaga aagcttaaaa gcgttaggta tcaatcatgt tgcagaatcc agattggata
     5341 acataattag tattgcagat caggatttaa catatacgtt attgcgtacg cctgccaaaa
     5401 aagagatttc agatatgata gaaaaagttg atatgagtat ccaaacagaa ttatctacca
     5461 tacatcaaat aaatgaagta gcagaagttt taggaaaaaa acataagatt ttattaatgg
     5521 tagattggaa agatggccgt gaaggtgttc tgacttatga tgttttagat tatattaaag
     5581 agattattca cttgaaaaat atccattttg taggtttagc attcaatttt atgtgtttta
     5641 aatctgatgc gccaagtgat gatgatatct ttatgattaa tcgattcgtc tcagctgttg
     5701 aaagagaaat aggatatcga ttgaaaatta tttcaggtgg taattcgagc atgttaccac
     5761 aacttttata taatgatttg ggtaaaataa atgaattgcg aataggtgaa acgttgttta
     5821 gaggtgttga tacaacgaca aatcaagcga tcgctatgtt atatcaagat gcgattacat
     5881 tggaggctga gattttagaa attaaacctc gagttaatac acaaacgcat gaatcattct
     5941 tacaagctat tgtagatatt ggttatttag atacaaaagt cgataatatt tcacctatgg
     6001 atcagcatat caatatttta ggcgcttcga gtgatcattt aatgcttgat ttaaatggtc
     6061 aaggacacta tcaagttgga gatcatatat ctttttcatt aaattatgaa gcactttctc
     6121 attcgatgta tatgaaaaat ttacataagg tatatattga tgattcaaag attgatacgc
     6181 tacttcaaaa ttttgatgtg aaatctcctg ctatggtaaa ccactattaa tatatttatc
     6241 aataagtcta agttgacaag ttatactaca tatcgtttgc acggttgtat cataattgtt
     6301 caacttagat tttttgtatt tgttgattta tcaaattaag tgcaacagtt cgtcacataa
     6361 aattgcaaca gataatatca gctgaattac agggataacg gtcatgctaa atggtgtcaa
     6421 ttgtattaat gcaaagatga tatagcaatg atacattatc agtattttgt ctaaggaaat
     6481 gtgctaattg tagtcataat tattagagga aatatatagt catacatttt agaaatacaa
     6541 aaaagattga acgctacttg acaatgataa ttgttatcaa taaaataata aatgaagtta
     6601 tacatattaa ggagtggaac gatgagaggt ctaaaaactt ttagtatatt gggattaata
     6661 gttgccttac ttttagttgc agcttgtggt aatacggata attcaagtaa aaaagaatca
     6721 tcaactaaag atactatttc ggtaaaagat gaaaatggta cagtaaaagt acctaaagat
     6781 gcaaaacgta tcgttgtatt agagtactca tttgcagatg cattagcagc attagacgtt
     6841 aaaccagttg gtattgctga tgatggtaag aaaaaacgta tcattaaacc agttagagaa
     6901 aaaattgggg attatacttc tgtaggtaca cgtaaacagc caaacttaga agaaattagt
     6961 aaattaaaac cggatttaat tatcgctgat agcagtagac ataaaggtat taataaagaa
     7021 ttaaacaaaa ttgcaccaac attatcatta aagagttttg atggagacta caaacaaaat
     7081 attaattcgt tcaaaacaat tgctaaagct ttaaataaag aaaaagaagg cgaaaaacgt
     7141 cttgctgagc atgataaatt aatcaataag tataaagatg aaattaaatt tgatagaaat
     7201 caaaaagtgc ttccagcagt agttgctaaa gctggtttat tagcacatcc aaactattca
     7261 tatgttggac aatttttaaa cgaactagga tttaaaaatg cattaagtga cgatgtaaca
     7321 aaaggtttaa gtaaatattt gaaaggacct tacttacaat tagacactga acatttagct
     7381 gatttaaatc cagagcgtat gatcattatg acagatcatg ctaaaaaaga ttctgctgaa
     7441 ttcaagaagt tacaagaaga tgcaacatgg aaaaagttga atgcagttaa aaataatcgc
     7501 gtggatattg ttgaccgtga tgtttgggca agatctcgtg gcttaatttc ttctgaagaa
     7561 atggctaaag aacttgttga attatcaaaa aaagaacaaa agtaaggtgg aagtaaatgg
     7621 ctataaaaga aataagtagc caatctgcca tagatcataa aagaaaaaga cgcacaacac
     7681 tcacgtatat agtgagtttg tgctttcttt ttatttgtat atatttaaat atggcgattg
     7741 gttcttcgaa aattaatttt agcgatatca ttcactatgt tactggtcat acagatacga
     7801 aagcaacgtt tttattgcat aatgtacgta tgccaaggat gattgcaggg ttatttattg
     7861 gcggtgcatt agcggtatct ggtttgttaa tgcaagcaat gacacgcaat ccgctagcat
     7921 cacctaaaat ttttggtgtc agttctggtg catcgttttt cattgtattt gtgacgatta
     7981 tcattccagc attagaatat tatgcattat atttaggtgt gataggtgca tttataggtg
     8041 gtttaactgt ttatacactt tcaggtgcta cgaaaggtat gacacccatt aaattagcat
     8101 tggctggtat ggccatccat ttgtttttta gtagtatgac cgaaggtatt attattttga
     8161 atgagaattc aaacgaacaa gtgatgtttt ggcttgtcgg ttcattatcc agtatgaagt
     8221 gggatgaaat tttaaccatt ctaccatgga ttattggtgc tttaattgta acgattttta
     8281 ttggacgtca actaacgata atggaactag gcgatgacat tgctaaggga ttgggtcaaa
     8341 atataaataa agttagaatt atcattggac tattagtcat tattcttaca ggtatgtcgg
     8401 tgtcagtggc aggaccaatt ggatttgttg gcttaatcgt accgcatata gtcaaacgct
     8461 atgtgagtaa gaattattta gtcatgattc cattaacatt tattataggt gcagatttgt
     8521 tactactatc agatgtatta agtcgtttaa ttacatatcc ttatgaatca ccagtaggga
     8581 ttgttacatc attcgtaggt gcactttact tcttatttat aacgattaaa ggggtgaaaa
     8641 gaatatgatg acaacgaaaa ttatacgtcg ttatagttta atcacaatcc ttttaattgt
     8701 gagtatattc ataagtttat gtgtagggtc tgtcatgatt catccgattg atgctattaa
     8761 gggaatcttt acacaagatg attttatttt aaatgagtat cgtataccta gaacattact
     8821 tggtattatt atcggtagta gcttggctat ttcaggtgca attatacaag ctgtaataag
     8881 aaacccgtta gcctcacctg atgttatagg tatttctaaa ggtgccagtt tagctgctgt
     8941 gattattatt atgacctttc caacagcacc attgtttgta ttacctatag gttcattctt
     9001 aggtgccttt gcagtaagtt tatttttatc atttttaatt tcaaaatatg atgttaaagg
     9061 ttcaaagctt gccttaattg gtttagcaat aggcgcaata tgtacggcga ttgtgcagtt
     9121 cttattaatc agaaatccac tggaagcaaa caaagcgctt gtttggttga caggtagtct
     9181 atatggacat gatatgtcga atatttatac tatattacct tggtttttaa taacgatacc
     9241 aatcatcatt ttattgagtt atcaattaga tgtattgaat ctaggtgatg ctgttgcaac
     9301 cgcactcggt ttaaaagtta aaactattaa aatgctattg ttaattttag cagtaatatt
     9361 agcaggatcc gcaatttcag ttgtcggtgg tattagcttt ttaggattaa ttgcacctca
     9421 tattgcgaga caattaatag gaaataaaac attacacgtt attatcatgt cggggttaat
     9481 tggtgctata ttgttaacat ttggagatgg tttagcccgt ggcattcatc cgccactgga
     9541 tattcctgtt ggggttatca ttgcaattat cggtgcacca tacttcttaa tattattacg
     9601 aagaatgaaa taactaacat atgattagag tttaaaaaag gttaagtacg aaatcagtaa
     9661 ttgtacttaa ccttttttga attcatcttg aaaaattaca agaaatagcg tatatttaat
     9721 aactgtaatt attcattatt ttgcatacat aaacattttg aaagatagaa tgataaggaa
     9781 tatgtttgtt aactgaatag aattctgtat atacttaaaa cgaatacata gagaaatgga
     9841 ttgattttat ggatagcttt tcaaggaaag agatagttat tgggagactt aagtttatta
     9901 cgatgtcttt aattggtatc ttattatttt tagtaccaat tccagtcgaa caagatggac
     9961 agaagcaaac aacgttgccc gtggcatttt tagcaggcgt gcttaaagat gtattaggtg
    10021 gcgtaatgcc atttttaatt gtgacgataa ttactttatc gggtatcata actctgattt
    10081 gttcaactat tttaaaagat aaattgaaac ctgacgggct aatgaacaat gcttttaacg
    10141 ttagaattgg ttggttaatt ttacgaatat tagcagttgt ttttgcatgg atgacatttt
    10201 tacgaattgg ttcaaaggtt atttactcag atgaaacggg tggattattg ttctcaagtt
    10261 tattgccaac attagtggca gtattcttat ttgcagctct atttttaccg ctattaatgg
    10321 aatacggttt gttagagatg ttgggtccta tttttagacc ggttatgaga cctttattta
    10381 cattgccagg acgttcgacg gtagataatt tagcatcatt tattggagac ggaactgttg
    10441 gtgtcttaat tacaagtaga caatatggtg aaggatatta ttctcgtcgt gaggcaacag
    10501 ttatttcaac gacatttagt gtagtttcta tcacctttgc aattgtagtt gctgaaacag
    10561 tgcacatgca aaatcaattt tttgctttct atttatcagt gatagtatcc tgcctcgttg
    10621 cagcagtcat tatgccaaga atttggcctt taaataaaat tcctgatgag tatgctaagg
    10681 aggtacctga aagtgcgcgt actgaggcat tgccagaagg aaagacggca ttgagacatg
    10741 gctttgatac tgcaacagaa gtaggtatta aagcaccggg cgttattgac ttttttaagt
    10801 caggattaaa aacagtcatt gatatgtggt ttgttatttt gcctgtagta atgagtattg
    10861 gtactattgc taccattatt gcgaactaca cgcccttttt tgtcatattg ggtaaacctt
    10921 ttgttccatt tttagagtta atgcaaatac ctgaagcggc acaggcttct caaacgatta
    10981 ttatcggttt tgccgatatg tttttacctt ctatattaat tgaaggtgta caaaatgata
    11041 taactcgttt tgttattggg gcacttagta tatcgcagtt aatatacttg tctgaagtag
    11101 gtggcgtcat acttggttcg aagattcctg taagcatcgg caaattgttt atgattttct
    11161 tgattagaac aatcattaca ctaccaatta tttctttgat ggcacatctt ttgttataaa
    11221 tatatgtgaa aaaggacaac tgttaaaatt atgggaacag ttgtcctttt gtatttgttt
    11281 atgctgggtg ttgaatcaat taaaaaaatc attcatgaag ccctcaccat gaatgatttt
    11341 tttatacaat ttatttagtt aattcattgt aaactttttg atcattttga gtataaataa
    11401 tgtattcgtg gtcttcagta tcgataataa cgcgattcgt tttaccaaac gttgagccga
    11461 ttctagatac atctttgctg tctaatttag gaactgcatg aatatcttgg tcttgtgtaa
    11521 tatttttaat ttcacttgta ggaattttga tatcagcaac tctccattga atacgcactt
    11581 cattattatc cttttttact gtcattgcca ttattcaaac acatccttta aatattttgt
    11641 aaatacatca taaacgattt tgtaatcgtt ttcaagagtt atgtgatact tttttaaatt
    11701 gtgtcatact aatgaatttt aacccgaaaa tatgttcgtg tttttgttag ggatgtggta
    11761 aaatgtgagt attaaaggag aacaaatatg acaggtaaaa cacatgcttc atgcggcatg
    11821 ctcgttggag cgctaacaac acaatatttt caaacggata ttttttcgtc ggttacggtt
    11881 atcattctag caactctagc tagcttatta cccgatatat gtcacactca aagtaagatt
    11941 ggcagaaggt ttaaggtaat tagttttttt gtgagattga tctttggaca tcgaaccttt
    12001 acgcattcaa tattatttat cgcaatcata gcatttttat tgcaaattat tcagacgcct
    12061 aattattata tggcagcaat tataattgga ttagtgtcac atgttatact agatatgata
    12121 acaccaaaag gcgtgaaatt attttatccg ttaccgttta atgtgaagac accgattcaa
    12181 tttaaaacag gtggacttgt agacttatct ttagctacag cgttaatggt cggaacagtt
    12241 tatgttttat ttcagccttt catcaatgat ataataagca actggaatac taagttttta
    12301 tagaaaataa attattaaag gttacaaact cagcaggagg cataaaatgc tagataaaaa
    12361 tcaattagct aaatataaac aagatcattt gtgtgaatat gaaaaaataa tgagtaacaa
    12421 tgaaaaagaa gcacttgaag agaaggtagc gtcattagat ttagatttta ttgccaaatt
    12481 atataacgat ttatacatca ataagaaaac aatagatgat gtatctgcag tttctgaagt
    12541 gaaatatgat attaaatcac aaatgagtga tgatgagatt aagcgtttag aggaacaagg
    12601 acttcaagct attaaagaag gacaatttgc agtacttttg atggcaggtg gtcaaggaac
    12661 aagacttggt tacaagggcc ctaaaggttc gtttgaaatt gaaggtgtaa gtttatttga
    12721 acttcaagct aaccaattaa aaacattgaa tcatcaatct ggacatacga ttcaatggta
    12781 tattatgaca agtgatatca atcatgaaga aactttagct tatttcgaag cacatagtta
    12841 ttttggatat gatcaagaag caatccattt ctttaaacaa gataatattg tggcgcttag
    12901 tgaagaaggg aagttaattt taaatcaaca gggtcgtatt atggaaacgc cgaatggtaa
    12961 tggtggcgtg tttaaatctt tagacaaggc aggatactta gaagaaatgt ctaataatgg
    13021 cgttaagtat attttcttga ataatatcga caatgtttta gtaagagtgt tggatccact
    13081 atttgcagga tttacggtag aacatgatta cgatattact tctaaaacga ttcaaccaaa
    13141 accaggagaa agtgttggac gtttagttaa tgtcgattgt aaagatacag tactggagta
    13201 ttcagaatta gatccagaag ttgcaaatca atttaacaat gctaatatcg gtattcatgc
    13261 atttaaactt ggttttattt taaacgctgt aaatcgtgag ttaccttatc atttagctat
    13321 taagaatttg aagcaattag atgagaactt tggtgttatt gaacaaccaa ctttaaaatt
    13381 cgaattgttc tattttgaca tatttactta tggaactagt tttgtcacgt tacaagttcc
    13441 tagagaagaa gaattttcac ctcttaaaaa taaagaaggt aaagatagtg ttgcaactgc
    13501 aacagaagat ttacgtagaa tgggtttaat ttaatgatag gcggtgacta tgatgtccaa
    13561 atcaactaaa aaaagtaatg acacaatcgt agaaacattt aaggatatca tccctttatc
    13621 atttggagaa gaaattggta atgcggcatc tcatggtgtt gcagctttgt taacattatt
    13681 agtgttaccc tatgcggctg tgcacagtta tattgcacat ggtacgcttg cgtctgtgag
    13741 tatgtctatt tatgttattt ctatctttat gatgtttatt tcatctacta tttatcattc
    13801 tatgcaaaat gaaacacctc ataaatatat tttaaggatt atcgatcaca gtatgattta
    13861 cgtagcaatt tctggtacat atacgcctat tttgttaaca gttgtaggtg gttggatagg
    13921 ttggacagta tttattttat tatggggaac gacactttgg gggattttat ataaatccat
    13981 agctgtcaaa gttaaccaaa agttaagctt aattgtctat ttagtgatgg gctgggtagg
    14041 tattatcttg ttaccaatta tttttattcg cacatcatgg atattcatat tattcatttt
    14101 cttaggtggt gtgtcttata caattggtgc atggttttat gcacaaaaga atagaccata
    14161 ttttcatatg atttggcata tttttattgt aatagcatca ctattgcatt taattgcaat
    14221 cttatatttt atgtagtcgt ttagaaaagc tgggggaaac tccgcttttt ttattacata
    14281 aatcattcta ataggggatt gtattgtaat agaattatcg ttaagatttg aaagtcaaaa
    14341 gtgtttatac atatgaaaac caattatttg taaataaaat cttataaata aatatataga
    14401 aggaggtatt tcatgcgatt aaagtcaatc ataactgtta tagctttaat attaattatg
    14461 tttatgtcag cgattgaatc atctattatt tcgcttgcat tacctacaat taaacaagat
    14521 ttaaatgcag gaaatttaat ttcattaatt ttcacagcgt attttatcgc attagtaatt
    14581 gccaatccta tagtgggcga attactttcg agatttaaaa ttatttatgt ggcaattgca
    14641 ggattacttt tatttagtat aggtagtttt atgtgtggtt taagtactaa ttttactatg
    14701 ctaattatat cacgcgtgat acaaggtttt ggatcaggtg tattgatgtc attatcgcaa
    14761 attgttccaa agttagcatt tgaaattccg ttacgatata aaattatggg catagttggc
    14821 agtgtttggg gtatttcgag tatcattggg ccacttctag gtggtggtat attagaattt
    14881 gcaacatggc attggttatt ctacataaat ataccgattg cgatcattgc cattatatta
    14941 gttatatgga catttcattt tccagaggaa gaaacagttg ctaaatcaaa atttgatact
    15001 aaagggctca cgctatttta tgtatttatt ggattgatta tgtttgcttt attaaaccaa
    15061 cagctgttac ttttaaattt cttgagtttt atcttagcta tcgttgtggc aatgtgttta
    15121 tttaaagtag aaaaacatgt ttcttcacca tttttacctg tggttgaatt taatcgttcg
    15181 attactttag tttttataac tgaccttcta acagctattt gtttaatggg attcaattta
    15241 tatattccag tctaccttca agaacaacta ggattatctc cattgcaaag tggattggtt
    15301 atttttcctt tatctgtagc ttggattaca ttgaatttta atttacatcg aattgaagca
    15361 aaactatcaa ggaaagttat ttacttacta tcatttacat tgctactagt aagtagtatt
    15421 atcatttcat ttggtattaa attgccggta cttatagcat ttgtgttaat tttggcagga
    15481 ttaagttttg gatatattta tacgaaagat agtgtgattg tccaagagga aactagccca
    15541 ttacaaatga agaaaatgat gtcattttat ggattaacta aaaatcttgg ggcatcaata
    15601 ggttcaacaa taatgggata tctttatgcg atacaatcag gaatctttgg tccaaactta
    15661 cacaatgtgt taagtgctgt tgctgtaatt agcattgggc ttattgtttt atgggtcgta
    15721 ttttttaaag aacagtcatc tcaatcaaaa gaatagaatt atgattaaaa ttgatgtagt
    15781 gtaattaaag taagaaataa taaatttcct agatactaga taaatcgttc aatattttat
    15841 taataaggag gccaatgatt gtgaattatc ttaaacataa gttttataac ttgttaacta
    15901 caatgattgt tctctttatt tttgtacttt ctggtgcgat ttttttaaca tttctagggt
    15961 ttggtttata tggattaagt agaatactta tttattttag gttgggtgac tttacatata
    16021 atagaagtat gtacgataac ctattatatt atggcagtta tataatcttt gggtacttca
    16081 tcatatttgc agtcgagcat ttaatggatt attttagaaa aatgttgcct gaaaatgcat
    16141 attttagagg cgcgactttt catttgattt cttacacagt tgcaacaacg ttattttatt
    16201 ttattattca tctgaactat gtgtatatca atatcgattt ttgggtcatc atggttatta
    16261 tagggttctt atatgtttgt aagcttcaat tttaccctga aagtaaaaat ttaaataata
    16321 gaaaatagat atctgaagtt gagggcacat atgtgttcca acttcttttt gttcaataga
    16381 agaatcgata atagtgagtc ttagtgctca atttgtggta aataagttaa ataaccattg
    16441 tatgagtctg agacataaaa taaattatta ggccctacaa tgttatattg ttagttttta
    16501 agtacacatt agctgtgcct aatgtgtaag aaccaataat gatttattat gtagtgcttc
    16561 tttattattt gagtactact cccattatgt tcatggctat acgtattaaa gggacaagtg
    16621 tatatctgtt ttaaaaagga ggaattatga tggctaaagt tgaattaaca acccggagac
    16681 gaaacttcat agttgctgtt atgttgatta gtgcgtttgt agctatttta aatcaaacat
    16741 tattaaatac agcgttacct agtataatga gagaattaaa tatcaatgaa agtacatcgc
    16801 aatggctagt tactgggttt atgcttgtta atggcgtcat gatacctctg acggcatatc
    16861 taatggatag aattaaaact agacctttat acttagcggc gatggggaca tttttattag
    16921 gttctattgt tgcagcctta gctccgaatt ttggagtttt aatgttagct cgtgtaattc
    16981 aagcgatggg tgcaggcgta cttatgccct taatgcaatt tacgttattt acattgttca
    17041 gtaaagaaca tcgaggtttt gcaatgggac tagcaggttt agtaattcaa tttgcaccag
    17101 caataggacc tacagctaca ggattaatta ttgatcaagc gagttggcga gttccattta
    17161 ttataatttt aggaattgct atacttgcct ttgttttcgg tttggtttca atctcgagtt
    17221 acaatgaagt gaaatatacg aaattagata agcgttcagt aatgtattca actattgggt
    17281 tcgggttaat gctatacgca tttagtagtg caggagattt aggatttaca agtccaatag
    17341 taataggtgc gttgatatta agtatggtta ttatctattt atttatacgt agacaattta
    17401 atattactaa tgcactttta aatttaaggg tttttaaaaa tagaacattt gcattatgta
    17461 cgattagttc aatgattata atgatgtcaa tggttggacc tgcgctgctt ataccgctat
    17521 atgttcaaaa cagtttatct ttatctgcct tgttatcagg acttgttatc atgcctggtg
    17581 caataataaa tggtattatg tcagttttta caggtaaatt ttatgataag tatggtccta
    17641 gaccattgat ttatactggt tttacaattt taacaattac tacaattatg ttgtgtttct
    17701 tgcatacaga cacatcttat acgtatttaa tagtcgtata tgcaattaga atgttttcag
    17761 tttctttact catgatgccg ataaatacta caggaattaa ttctttgaga aatgaagaaa
    17821 tctcacatgg cacggctatt atgaactttg gtcgtgtaat ggctggttca ctaggcacag
    17881 ctttaatggt tacattaatg agttttggtg caaaaatatt tttatctaca tcgccatcgc
    17941 atttaactgc aactgaaatt aaacagcaat ccattgctat aggggtggat atctcatttg
    18001 cttttgtagc tgtgcttgtt atggcagctt atgtgatagc actttttata agagaaccta
    18061 aagaaataga aagtaataga aggaaatttt aaaataatta tagtagttgg tctatttaaa
    18121 ataataggct aactgctttt tttatttaat aaaaagtttt atacttttag tgatagacta
    18181 agcaaaaatt gttatttgct atgatgtaga tgtcttaaaa tgattaaggg ggatttgctt
    18241 tgttaacggt agatcaagtg aaagaattgg taggagaaat taaagatcct attatagatg
    18301 tgcctttaaa agaaacagaa ggtattgttg aagtttctat taaggaagaa aaagaacatg
    18361 tgagtgttaa attagcaatg gcacaattag gtggtgcacc gcaattagat ttacagatgg
    18421 ctgttgttaa tgcattaaaa gaaaacggtg cgaaaacggt cggtatacga tttgaaacat
    18481 tgccggaaga aaaagtaaat caatttaaac caaaagaaga aaataaacct aaaacgatag
    18541 aaggcctatt atctcaaaat aatccagtcg aatttattgc aatagcctcc ggtaaaggtg
    18601 gtgtcggtaa atctactgtt gcagtaaatt tagccgttgc cttagctcgt gaagggaaaa
    18661 aagtcggatt agtagatgcc gatatatatg gatttagtgt accagatatg atgggtattg
    18721 atgaaaagcc tggaattaaa gggaaggaag taattccagt tgaacgtcat ggcgttaaag
    18781 ttatatcaat ggcctttttt gtggaagaaa atgcgccagt tatatggaga gggccaatgt
    18841 taggtaaaat gttgacgaat ttctttacag aagttaaatg gggagacatt gaatatttaa
    18901 tactcgatct tccacctgga acaggagatg tagctttaga tgttcatacg atgttacctt
    18961 caagtaagga aattattgta acgacacctc atcctacagc agcatttgtt gcagctcgcg
    19021 caggtgcgat ggcaaaacat acggatcatt ctattcttgg cgtaattgaa aacatgagtt
    19081 attttgaaag taaagagacg ggtaataaag aatatgtctt tggcaaaggt ggcggtacta
    19141 agttagctga tgaacttaat actcaattac ttggggaatt acctttagag caaccatctt
    19201 ggaatccaaa agattttgca ccgtcaatat atcaatcaga tgatcgtcta ggtaaaattt
    19261 atagctcgat tgcacaaaaa gttatagctg ctacaaataa ataggtataa tatatgctaa
    19321 acgtacaaaa ttacttttat tttgtacgtt tttatgttga tttgttttgg aatgctggta
    19381 aaataagttc ttgtgttaag caataaacgc gttgaaaaag aagcgggttg tgaacacaaa
    19441 aagtttttaa aaaagttatt gactttaaat tccaaagttg ataatatgat aaagtcgtca
    19501 aaaacgacgc agttgttttt aaagaagaat ttcaaaagtg taaaacttac cattgaaata
    19561 actataaaaa caatatagaa taaataaagt aatcgttcaa aaagttattg acttcaagaa
    19621 ataaatgaag tagaataaaa gagattgaac attaatgaac attgaaaact gaatgacaat
    19681 atgtcaacgt taattccaaa aacgtaacta t
//
LOCUS       DALAAR010000049        19410 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000049 DALAAR010000000
VERSION     DALAAR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.051"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            81..1775
                     /gene="ezrA"
                     /locus_tag="O2458_001828"
     CDS             81..1775
                     /gene="ezrA"
                     /locus_tag="O2458_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCZ3887691.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNTEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(1849..1989)
                     /locus_tag="O2458_001829"
     CDS             complement(1849..1989)
                     /locus_tag="O2458_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887692.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            2138..3286
                     /locus_tag="O2458_001830"
     CDS             2138..3286
                     /locus_tag="O2458_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ3887693.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            3286..4509
                     /gene="thiI"
                     /locus_tag="O2458_001831"
     CDS             3286..4509
                     /gene="thiI"
                     /locus_tag="O2458_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCZ3887694.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPKFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            4553..5323
                     /locus_tag="O2458_001832"
     CDS             4553..5323
                     /locus_tag="O2458_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCZ3887695.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            5421..5915
                     /gene="tpx"
                     /locus_tag="O2458_001833"
     CDS             5421..5915
                     /gene="tpx"
                     /locus_tag="O2458_001833"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCZ3887696.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            6038..6985
                     /locus_tag="O2458_001834"
     CDS             6038..6985
                     /locus_tag="O2458_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3887697.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            7073..8275
                     /locus_tag="O2458_001835"
     CDS             7073..8275
                     /locus_tag="O2458_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCZ3887698.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(8521..9021)
                     /locus_tag="O2458_001836"
     CDS             complement(8521..9021)
                     /locus_tag="O2458_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ3887699.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            9162..10280
                     /gene="ald"
                     /locus_tag="O2458_001837"
     CDS             9162..10280
                     /gene="ald"
                     /locus_tag="O2458_001837"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ3887700.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVRGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            10404..10502
                     /locus_tag="O2458_001838"
     CDS             10404..10502
                     /locus_tag="O2458_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887701.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(10596..11651)
                     /locus_tag="O2458_001839"
     CDS             complement(10596..11651)
                     /locus_tag="O2458_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ3887702.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            11826..12515
                     /locus_tag="O2458_001840"
     CDS             11826..12515
                     /locus_tag="O2458_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ3887703.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(12820..13233)
                     /locus_tag="O2458_001841"
     CDS             complement(12820..13233)
                     /locus_tag="O2458_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ3887704.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            13438..14736
                     /locus_tag="O2458_001842"
     CDS             13438..14736
                     /locus_tag="O2458_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCZ3887705.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            14997..15938
                     /gene="pde2"
                     /locus_tag="O2458_001843"
     CDS             14997..15938
                     /gene="pde2"
                     /locus_tag="O2458_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCZ3887706.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            15959..19156
                     /locus_tag="O2458_001844"
     CDS             15959..19156
                     /locus_tag="O2458_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ3887707.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT         6832 a         2699 c         3584 g         6295 t
ORIGIN      
        1 atggcaataa attcatttta taatttatcc ttaagcaaaa ttacgttata atagtaatga
       61 taaattagga ggagaagcat atggtgttat atatcatttt ggcaataatt gtgattatat
      121 tgattgctgt aggtgtatta ttctatttac gttcaaataa acgacaaatt attgaaaaag
      181 caatcgaacg taaaaatgaa attgagacgt taccttttga tcaaaacctt gcacaattat
      241 ctaagttgaa tttaaaaggt gaaacaaaaa cgaaatacga tgcaatgaaa aaggacaacg
      301 tagaaagtac aaataagtat ctagctcctg tggaagaaaa aatccataat actgaggctt
      361 tattagataa atttagtttc aacgcatctc aaagtgaaat tgatgatgca aatgagttga
      421 tggatagtta cgaacaaagc tatcagcaac aattagaaga tgtaaatgaa attattgcgt
      481 tatacaaaga taatgatgaa ttatatgaca aatgtaaggt tgattatcgt gaaatgaaac
      541 gtgatgtttt agcaaatcgt catcaatttg gtgaggcagc aagtctactt gaaactgaaa
      601 ttgaaaaatt tgagccaagg ttagagcaat atgaagtact aaaagctgat ggtaattatg
      661 tacaagcgca caaccatata gctgccttga atgaacaaat gaaacagcta agatcttata
      721 tggaagaaat accagaatta attagagaaa ctcaaaaaga attacctggt caattccaag
      781 atttaaaata tggttgccgt gatcttaaag ttgaagggta tgatctggat cacgtgaaag
      841 tagacagtac attacaaagc ttaaaaacag agcttagttt cgttgaacca ttaattagcc
      901 gcttagaatt agaagaagct aatgataaac tagctaatat caatgataag ttagatgaca
      961 tgtatgattt aattgaacat gaagttaaag ctaaaaatga tgtcgaagaa acaaaagata
     1021 tcattacgga taacttattc aaagctaaag acatgaatta tacattgcaa acagaaattg
     1081 aatatgtacg tgaaaactac tatataaatg aatctgatgc tcagagtgtt cgtcaatttg
     1141 aaaatgaaat tcaaagttta atttctgtat atgatgatat tttaaaagaa atgtctaaat
     1201 ctgctgtgcg atatagcgag gttcaggata atttacaata tttagaagat catgtcacag
     1261 ttattaatga caaacaagaa aagctacaaa atcatctgat tcaattgcgt gaagatgaag
     1321 cagaagcaga agacaatctg ctacgagtac aatcgaagaa agaagaagtg tatcgtcgat
     1381 tacttgcttc taacttaaca agcgttcctg aaaggtttat catcatgaaa aatgaaattg
     1441 atcatgaagt tcgtgatgtt aacgaacaat ttagtgaacg tccaatacac gttaaacagt
     1501 taaaagataa agtgtctaaa attgtgattc aaatgaatac atttgaagat gaagcaaatg
     1561 atgttcttgt taatgctgtt tatgcagaga aattaattca atatggaaat agatatcgta
     1621 aggactatag caatgttgat aagagcttaa atgaagctga acgattattt aaaaataatc
     1681 gctataagcg tgcgattgaa attgcagagc aagctcttga aagtgttgag ccaggtgtta
     1741 ctaaacatat tgaagaagaa gttattaagc aatagaaact agtatgtagt tatacttaaa
     1801 taatatgagc actctgtcaa attggactga tgagtttaat aattgaagtt agccaacgat
     1861 acgttgtcta gcttcttttt tatatggata aatgaaaggg acaataaata taaatagcaa
     1921 ttgtttaaag ataaacgtaa tcaaatgtgt tgttttaatt aatataagta gtgaaaaaag
     1981 cataatcaca cagctgttta aatagagtga aatagtctaa ttcttattta ataagtagaa
     2041 ataagattat tttgcgctat aacctttatt ttttgtcgga taatagtata atgcttgagt
     2101 aaaacaattt taacgattga tgattaatga aagagaagtg tttattttga tatatctaga
     2161 taatgcggca acgacgaaag catttgaaga agtgttagat acttatttaa aagtaaatca
     2221 atcaatgtat tataatccga atagtccgca taaagctggt ttgcaggcaa atcaattact
     2281 acaacaagca aaaacccaaa ttaatgcaat gattaattca aaaacaaatt atgatgttgt
     2341 attcactagt ggtgcaactg aatccaataa tcttgcttta aaaggtattg cctatcgtaa
     2401 atttgataca gcgaaggaaa taattacatc cgtgttagag catccgtccg tattagaggt
     2461 tgtaagatat ttggaagcac acgaaggatt taaagttaaa tatgttgatg taaagaaaga
     2521 tggcagtatt aacttagaac acttcaaaga attaatgtca gacaaagtcg gtttagtaac
     2581 atgtatgtat gtaaataatg taactggaca aatacagcct attccacaaa tggctaaagt
     2641 tataaaaaat tatcctaagg cacattttca tgtagatgcg gttcaagcat tcggcaaaat
     2701 ttcaatggat ctcaataaca tagatagtat tagtttaagt ggacacaagt ttaatggttt
     2761 aaaaggacaa ggcgtcttac ttgtaaatca cattcaaaat gttgaaccaa ctgtccatgg
     2821 tggtggtcaa gaatatggcg ttagaagtgg aacagttaat ttgccaaatg atattgcaat
     2881 ggttaaagcg atgaagatag ctaatgaaaa ctttgaagca ttgaatgcat ttgttactga
     2941 gttaaataat gacgtccgtc aatttttaaa taaatatcat ggagtttata ttaattcttc
     3001 aacttcaggt tcaccattcg ttttaaatat tagttttcct ggcgtaaaag gtgaagtatt
     3061 agttaatgct ttttcaaaat atgacattat gatatctacg acaagtgctt gttcatctaa
     3121 acgtaataaa ttaaatgaag tattggctgc aatgggatta tcagacaaat ctattgaagg
     3181 tagtataaga ttatcatttg gggctactac aactaaagaa gatatagcga ggtttaaaga
     3241 aatatttatc atcatttatg aggaaattaa ggagttgcta aaataatgaa gtatgatcac
     3301 ttgcttgtta gatacgggga gttaacatta aagggttcaa atagaaagaa atttgtaaat
     3361 caattaagaa ataatgtaaa taagtcatta aaaggacttg atgggtttgt cgttaaaggc
     3421 aaacgagatc gtatgtatat tgaacttgaa gaccatgcag atataaatga aataacatat
     3481 cgattatcaa aaattttcgg tattaaatct attagtccag tattaaaagt agaaaaaaca
     3541 atagaggcaa taagtgcagc ggcaattaaa tttgcgcagc aatttgaaga aaacagcaca
     3601 tttaaaattg atgtgaagcg tgccgataaa aatttcccaa tggatacgta tgaattacag
     3661 cgtgaattgg gtggtgcagt attgaagcac ttcgacaata tttcagtgaa tgtcaaacgt
     3721 ccagatcatg aaattcgagt ggaagttaga ttagatgcaa tttatatgta tgaagaagtt
     3781 gttccgggtt caggtggatt accagttggt actggtggta agacgttact aatgctttca
     3841 ggcggtatag actcaccagt tgctgggatg gaagtgatga gacgtggcgt aacaattgaa
     3901 gcgattcatt tccatagtcc accatttaca agtgatcaag caaaagaaaa agttattgaa
     3961 ttgacacgta ttttagctga acgtgttgga ccaattaaat tgcatattgt accatttaca
     4021 gaattgcaaa aacaggtaaa taaagttgta catccaagat atacaatgac ttcaacgaga
     4081 cgtatgatga tgcgtgttgc tgataaatta gtacatcaaa taggggcttt agctattgta
     4141 aatggtgaaa acctagggca ggtagccagt caaacacttc atagcatgta tgcaattaat
     4201 aatgtaactt ctactcctgt attacgtcct ttattaactt acgataaaga agaaattatt
     4261 attaaatcga aagaaattgg tacatttgaa acatctattc aaccatttga agattgttgt
     4321 acaattttca cccctaaaaa tccagtaacc gaaccaaaat ttgataaggt agtccaatat
     4381 gaaagtgtct ttgattttga agagatgatt aatcgtgctg ttgaaaatat tgaaacactt
     4441 gaaataacta gtgattataa aactattaaa gaacagcaaa caaaccaatt aataaacgac
     4501 tttttataaa taaaatccta gagtaaattt aaacataagg ggatgttaaa ctatggattt
     4561 gaacttaacg atgattataa tcataatttt atttggtttt atcgcggcgt ttatagattc
     4621 ggttgtaggg ggtggcggtt taatttctac gccagcatta ttagcaatcg gtctaccacc
     4681 atctgtggct ttaggtacaa ataaattggc aagttcgttt ggttctttaa ctagtacgat
     4741 aaagtttata aggtccggta aagtggactt atatgttgtt gccaaattat ttggttttgt
     4801 atttttggca tctgcatgtg gcgcatatat tgcaacgatg gttccgtcac aaatattgaa
     4861 acctttaatc atcattgcac tttcgtcggt gtttatattc acattactta aaaaagattg
     4921 gggcaataca cgcacgttta ctcaatttac atttaagaaa gccataatat ttgcagcact
     4981 ttttatatta atcggctttt atgatggatt tgtaggtggg ggaacaggtt catttatgct
     5041 ttttgtattg ttagtctttg gttttgattt tttaagtgca gcaggaaatg ctaaagtttt
     5101 gaactttgct tctaatatag gtgcgcttgt attatttatg gtattaggac aagtagatta
     5161 tgtaataggt ttaattatgg ctataagtat gattgctggg tcatatgctg gggctcattt
     5221 tgctattaaa caaggtgttg gttatgtaaa agtattattt attattatta ctgctatttt
     5281 aattttaaaa aatgcttttg attatattca acaatttgta taaaattaaa tttgcttttg
     5341 attaaagcat gaaaattgta atcaaaccat aaattgtcgt atgatgtagt tagaatttta
     5401 aaatgcagga ggtcaagtat atgactgaaa taacattcaa aggtggacca atccacttaa
     5461 aaggtcaaca aattaatgaa ggtgattttg cacctgattt tacagtgtta gataatgact
     5521 taaatcaagt aacattagca gattatgctg gtaaaaagaa attaattagt gtggtaccat
     5581 caattgatac aggtgtttgt gatcagcaga ctcgcaaatt caactctgat gcttctaaag
     5641 aagaggggat tgtgcttaca atttcagcag acttaccatt cgcacaaaaa agatggtgcg
     5701 cttcagcagg tttagacaat gtcattacat taagtgacca ccgtgactta tcatttggtg
     5761 aaaactatgg cgttgttatg gaagaacttc gcttattagc tcgtgcagta tttgtattag
     5821 atgcagataa taaagttgtt tataaagaaa tcgttagtga aggtactgat ttcccagatt
     5881 ttgatgctgc tttagctgca tacaaaaata tttaatcatt aaagagataa atcttaaaat
     5941 gtatacatcg tgtccatcgt tgtcaacagc attaaaatag aattgttttc tatgattgct
     6001 aagacctatg ggcacttttt attggagagg gacgaatatg gcagaacaac aaacaattat
     6061 ggaacgcttg tttcatacat tagatgaaaa agctaaaaca ttaaataatg aaaatggcca
     6121 aagttttatt gaaaatcttg ggctagcaat ggaacaagta tataccaatg aaagaggatt
     6181 gttagaacaa tcgacgttac aagatcgacg taaagcattc caatttgcat atttaagttt
     6241 aatgcaggaa gaaaagatac aagcaaatca tcaaattaca ccagattcaa ttggattgat
     6301 actaggattt ttagttgagc gttttatgaa caaccaagaa gaattacata ttgttgatat
     6361 tgcaagtggt gccggtcatt taagtgctac tgtaaaagaa gtgttacctg aaattgcggt
     6421 tatgcatcat ttaattgaag ttgatccagt tttatcacgt gttagtgtac atttagcaaa
     6481 cttcttagaa attcctttcg atgtgtatcc tcaagatgcc atcatgccac taccattaga
     6541 agaagcagat atcgttattg gtgattttcc agtaggctat tatccaattg atgaaagaag
     6601 taaggagttt aagctaggtt ttgaagaagg acatagttat tcacattatt tattaataga
     6661 acaagcaata aatgcattaa aagatgctgg atatgccttt ctagtggtac caagtaatat
     6721 ttttacaggt gaacatgtaa aacagcttga aaaatatatt gcaacagaga cagagatgca
     6781 agcattttta aatttaccac caactttatt taaaaatgaa aaagcgcgaa aatctatatt
     6841 aattttacaa aagaaaaaat cgggtgaaac aaagccagtt gaagtattat tggcaaatat
     6901 tcctgatttc aaaattcctt cacaatttca aggatttatg acagagttaa atcagtggat
     6961 ggacacaaat cgtcctaaaa aataatctgt aatagtatag tcataaaact gtatgaatga
     7021 taaaatgaaa aatgaaatag tataaaaagc aaacgatgga ggatcattac ttatgtcaaa
     7081 attaatcttg gctatcaatg ctggtagttc atcattaaaa tttcaattaa ttagaatgcc
     7141 tgaagaggaa ttagtaacaa aaggtttagt agaaagaatc ggattgaagg attcaatttt
     7201 tacaattgaa gtcaacgggg aaaaagttaa aacagtacaa gatatcaagg atcacgttga
     7261 ggcagttgat atcatgttgg atgcttttaa agcgcacaat attattaatg atattaacga
     7321 tatcgatgga acaggtcacc gtgttgtaca tggtggtgaa aaattccctg aatcagtagc
     7381 tattactgat gaggttgaaa aggaaattga agaattaagc gaattagcac cgcttcacaa
     7441 cccagctaac ttgatgggta ttcgtgcttt ccgtaaatta ttaccaaata tccctcatgt
     7501 ggcaattttt gatacagcat tccatcaaac aatgcctgaa aaagcatatc tatatagctt
     7561 gccatatcat tattataaag attatggcat tcgtaagtat ggtttccatg gtacaagcca
     7621 taaatttgta tcacaaagag cggcagaaat gttagataag ccaattgaag atttacgtat
     7681 tatttcatgt cacattggta atggagcatc aattgcagct atcgatggcg gtaaatctat
     7741 tgatacatca atgggcttca caccacttgc tggtgtaaca atgggtactc gttctggtaa
     7801 tattgaccct gctttaattc catttattat ggaaaaaaca ggtaaaactg ctgaacaagt
     7861 attggaaatc ttaaacaaag aatcaggttt attaggatta tcaggtacat ctagtgattt
     7921 acgtgactta tcagaagaag ctgaatcagg taaagcacgt tcacaaatgg ctttagatgt
     7981 atttgcttct aaaattcaca aatatattgg atcatatgct gcgagaatgc atggtgttga
     8041 tgttatcgta tttacagcag gtatcggtga aaactcagta gaaattcgtg cgaaagttct
     8101 tgaaggctta gaatttatgg gtgtttactg ggatcctaag aaaaatgaaa acttactacg
     8161 tggtaaagaa ggatttatta actatcctca ttcaccagtt aaagttgtcg ttattccaac
     8221 agatgaagaa agtatgattg cccgtgatgt tatgacattc ggtggtttaa aataattttt
     8281 aaataaatat taagcgtcta tctccgctac aattatatgt aggaggtaga cgctttttgt
     8341 attgtttgaa aattaactag tgtaatgtct cttttcacga atatcttaaa gaattaagta
     8401 gtcgaagaag attatgaaaa cattattaag tgagttttat ttataaacaa taaaaaacca
     8461 agtgcagaaa gttgcacttg gttcataatg atcaagtagt tatctaatga tcatctatat
     8521 ttaattttga tatttttcac gtaattgagt tgttgcaact tgtggttgga agtctgctgg
     8581 taactcttca gtacgaacaa ctaacacgtc acatggcgca tgacgaacga tagattctga
     8641 tacagaacca acaataaatc tttccacggc atttaagcct gatgtaccac tcataatcaa
     8701 gtctgcatta atttcatgtg caagcttttt aggaatgata gatttaggag agccaaactc
     8761 tagacgcgtt tctacatctt taacaccagc gttagtagct acttctttat aaccatttaa
     8821 taattcttct gcaaaatgct tagatttttc agtaaattga gcatcataaa cttcataaga
     8881 agaatacgtt cttgaatcaa ttacattcac aattgttaac ttcgcatcgt tacgtttagc
     8941 aacaccaact gctctgttaa atgcccattc cgcttcatgt gaaccgtcaa ctgcgattaa
     9001 aatatttttg taagtaatca tagtaagtgc ctccttgttc tttcttaatt atattttacc
     9061 acaattttcg gaaaagtcag tgtaataacc tttacaattt tgtggatttt tgattgcgct
     9121 ttcaaaatgt tcgcgttaag atgattatgg aggtggatgg tatgaaaatt ggtataccaa
     9181 gggagattaa aaataatgaa aatcgtgttg gtttatcacc aagtggtgtg cacgctttag
     9241 tagaaagtgg gcatactgtt ttagtggaaa caaatgcggg ttcaggatca ttctttgaag
     9301 atgtagatta caaagaagca ggtgctgaga ttgttgctga acaagcaaaa gtttgggatg
     9361 tggatatggt tattaaagtt aaagaaccac ttgaatctga atatccatat tttaaagaag
     9421 ggcttgtatt attcacttat cttcatttag caaatgaaga aaaattaaca caagctttga
     9481 tagatagaaa agtaattagt attgcatatg agactgtgca gttaccagac cgatctttac
     9541 cattgttatc accaatgagt gaggtagcag gaagaatgtc agctcaagtt ggcgcagagt
     9601 tcctacaaaa acttaatggt ggtatgggaa ttctactagg tggtgtccga ggagtaccta
     9661 agggtaaagt aactattatc ggtggtggtc aagcaggaac aaatgcagct aaaattgcac
     9721 taggactagg tgcagatgtt acgattttag atgttaatcc aaagcgttta caacaattag
     9781 atgatttatt cggtggacgt gtacatacaa ttatgtcaaa tccgttgaat attgagttgt
     9841 atgttaaaca aagtgattta gtaataggtg cagttttaat tccaggtgct aaagcgccaa
     9901 gacttgtaac agaagacatg attaaacaaa tgaaaaatgg gtcagttatt attgacattg
     9961 ctattgatca aggcggtatt tttgaaacaa ctgataaaat tacgacacat gatgatccta
    10021 catatattaa gcatggtgtg gttcattatg cagttgcaaa tatgccaggt gcagtaccgc
    10081 gtacttcgac gttagcttta aataatgcta cgctacctta tgcgctcatg ctagctaata
    10141 aagggtatag agaagcattt aaatcaaatc aaccattatc attaggttta aatacttaca
    10201 aaggtcacgt aaccaataaa ggcgttgcag aggcatttga aatggaatat aaatctgtag
    10261 aagaagcatt acaattataa tttgaatttg atgaaaaggg aatggattac agcgtactaa
    10321 agaatgacgt tatcatctcc ttggttatta aaattaaata atgataaatt aaagctagaa
    10381 acaatgcgaa gaaatcatcg aacatgaatg atattgattt gattcattac atgttactga
    10441 tgaacatacc tgcatatgtg tttctagctt gtttgttaca tattgcattt agacttggat
    10501 aaaaatttat gtcttaggcg ctacaatgtt atattggcag tgtttgactg aatgagaatg
    10561 cgcttgtaac aagcttttca tttttatccc actccttatt tttcgtaatg tgttaatact
    10621 tcatatcctt cattagtgac aagtatgtca tcttcaattc ttacacctgc aacaccaggt
    10681 acataaatac ctggttcgat tgtaataacc atgccagctt ctaacaaatt agaattagta
    10741 cttgaaacat cttgatattc atgttcttgt aatcctaggc catgacctaa gcgatgaggg
    10801 aaatattcac cataaccttt ttctgaaata atatttctag cgatatgatc gatatctttt
    10861 aatggtattc caggtttaat tgcttggatt gcagatgttt ctgcttctaa tacaatatta
    10921 taaatttctt gtgcttcttt gctaggttca ccaaatttaa tagtacgtgt catatcgcta
    10981 caataatgct cataaattac acctaaatca aatagtacat attcattgct ttttaatctg
    11041 cgatctcctg gtgtgccatg aggtgatgcg gcatgatctc caaataaaac catcgtatca
    11101 aaactcattt cattgacgcc atattgtttg atagtttgct caatatggtt gactacttca
    11161 cattcagtca caccttcttt taaataagaa acacctattt cgatacactt atctgctaac
    11221 tcagcagctt tacgtatttt gctaatttca tcttcggatt taatatttct caattgtttg
    11281 attgttaaat caacatctcc gaatgaattg acattgaaac cagagattaa ttgtttttgg
    11341 cgtgctactg ttaagtgctc gctttcaatt agtaatttat tgattgtttg aggataaagt
    11401 gaaaaagggt tttcagtgtc taaatatcca acgatttcac ctgtgaaagg tgatgctttg
    11461 acttcttcga cttccatttt tggacaaaat agtacttgtt taccatcttt cttaatcaat
    11521 aatgcaaata atctttcatg gggttcgcta cggtatccag taaaataata tacattcaac
    11581 ggtgttgtaa tccatgctgc atcagcttgt tgattgttca attcgtctat tatttttgat
    11641 atttttgtca ttttataaac actcctcaac ataatattac ttccatttta tgccaattat
    11701 tattagattt caaaatcgag ctaatacatt aactagattt tcaattttta aaatttgaga
    11761 atttgttata attaagtggc aaaagggtac taaatttata tagtaattga taggaggatg
    11821 ctcaaatgaa gttatcattt catggtcaat caacgattta tttagaaggg aacaacaaaa
    11881 aggttatcgt agatccgttt atttcaaata accctaagtg tgatttgaat attgaaacag
    11941 tgcaggtaga ctatatcgtt ttaacgcacg gtcattttga tcattttggt gatgttgtgg
    12001 aactagcaaa gaaaacagga gcaacagtaa ttggaagtgc agaaatggct gactatcttt
    12061 cttcatatca cggtgttgaa aatgttcatg gtatgaatat tggtggtaaa gcaaactttg
    12121 atttcggaag cgttaaattt gttcaagcat ttcatagttc aagttttaca catgaaaatg
    12181 gtattccagt ttatttaggt atgccaatgg gtatcgtgtt tgaagttgaa ggaaaaacaa
    12241 tttatcatac tggtgataca ggtctgttta gtgacatgtc acttattgcg aaacgtcatc
    12301 cagttgatgt atgttttgta ccaattggag ataattttac gatgggtatt gatgatgcaa
    12361 gttatgctat taatgaattt attaaaccaa aaatttctgt accgattcat tatgatacat
    12421 tcccattaat cgaacaagat ccacaacaat ttaaagatgc tgttaatgta ggggacgtgc
    12481 aaattttaaa accaggtgaa tctgttcagt tttaaataga atatagattt tacactaaaa
    12541 aacacctcgc taatctatgt ggaagttatc gtctgcatat ttttagtggg tgtttttata
    12601 ttctactgat tcagttacga cgtggacggg ggacatcagt ggttctttat catttctgac
    12661 ctactctcgt aattttagtt ttatacaatt agacattgat tataaattta ctacgatata
    12721 aaaaatccag aaccacagtc ataatcgttt cttgtattta ctcagtcgtt tgagactgca
    12781 tcatgattga aagattattt gtgattctgg ataaaaattt tatttaacga ttaatacagg
    12841 gatagtcgca cgttttgcta ctttgtggct gacacttcca agtacaaatt tctttttgtc
    12901 ttctgcttta cggttactta aaacaacaat ttcgtattta ccgctattag catgttttaa
    12961 tagttcttct tttgcattac cacgaacaat tatttggtca taatcaatac cataatcttc
    13021 taaagctttg cgtgtttttt ccaatcgctt gcttcgttct tcagtaagtt tgtttaaatg
    13081 aacacctgct ttaattgatg cttgagcatc ttgttcgctg attgcgttta aaactgttac
    13141 gactgtacct tcgccagcta atttagacac ttcttttagt gctttttcat tttttaactg
    13201 agtgtctaca ccaagtaata tatttttata cattagtaca tccctcctca cttacattct
    13261 aataaataat aatggatttt tccatttaaa attaaagatt ttgtgatctt tgtgtttgtt
    13321 gtatattttc aaaaaataaa ataggtgaaa cgatcattat ttcatttcac ttcaatgctt
    13381 tttatccaaa ataaagttat aatgtagtta atactgttcg aaaagaagag gtaaagaatg
    13441 acaaaacatg aacaaatttt agattatatt gaatcgttat ctataggctc taaaatttcg
    13501 gtgcgtaaaa tcgctaaatt tttaaacgta tctgaaggga cagcatatcg tgcaattaaa
    13561 gatgctgata aaatgggcat ggtcgcaacg atagatcgtg ttggtacggt tcgaatcgaa
    13621 aaacgtaatc gtaatgaaat tgaacattta acttttaatg aaattgtcaa tattattgat
    13681 ggccaagtgt taggtggcaa caaaggtatc actaagatgg tatctaaatt tgcaattgga
    13741 gcaatggaac tcaaagatat tttaaagtat attggtccaa agacattatt gattgtcgga
    13801 aatcgtgaag atgtgcagat tgaagcttta aaaagaggta cggctatatt aattactggt
    13861 ggatttaagc catctaataa agttattaat tttgcgaatg aacatgattt acctgttcta
    13921 tcatcaagtt atgatacatt cctagttgca aatattatca acaaagcact ttttaatcag
    13981 aaaatacgaa aagatatctt aatagttcaa gatataatga cgccattaga tgatttatct
    14041 gtgctgtttg atacgatgaa aatagcagat tataagcgta tggcgaatcg aactggccat
    14101 acaagatttc cggtagtcaa tgagtcgtat aaattagtag gtattgtaac aagtcgagag
    14161 atgattaata caaaggacga cgacgaaata gataaagtga tgacgcgaaa tcctatttat
    14221 gtaaatgcta tgagtacagt agcaagttgt gcgcatatga tgatttggga aggtattgaa
    14281 ttgattcctg tcgtttcgag taataaaaag acagtgggtg tgattaatag acaagatgtt
    14341 ttaaagtcta tgcaactttt aggaagacag cctcagatgg gtgaaacaat taacgatcaa
    14401 atcgccaaat atataacaat gaatcaagat ggaattacag tagaagtttc accactttta
    14461 atcaatcatt acggaacagt aagtaaagcg gcatttgtaa gtattataga agaaacaata
    14521 caatatgaaa tgcgtaaatt taaaaaaggt aatgtcatga tagagaactt aaatatcgtg
    14581 tatattaaaa cagtacctat tgaatcacat ataacagtgc gctttggcat actagacgtc
    14641 ggaagaaact tcgccaaaat agaagtgaac atgcatagtc aaaacgacaa agtagcgagc
    14701 gctctcgtga tatgccaaat gtttgacgaa gtataggaac ttagcagaga aaataggcat
    14761 aagatagatt tcgcacaaac gttggggccc caccccaact tgcattgttt gtagaaatta
    14821 ggggtccaat ttctctgtgt tggggcccct gaactaaata gtatgaaaat atatatatct
    14881 aaataattaa aacgggcaaa tagtttaata tggtagtaac aatttttata atttagatat
    14941 tttgatatag actaatgctt aaattaagca acttttctaa aaaagggagt atgaaaatga
    15001 ttagtacttt gaatgaaatt atgaaatgta tcgaagacaa cgatacaatt attatacaca
    15061 gacatgttag accagatcct gatgcatatg gttcacaact tggtttaaaa tactatattc
    15121 agcagaaatt tccgcaaaag caagtatttg cagtgggtga agcggaatca tcattaagtt
    15181 ttattggaga gttagataac attgatgata aaacatatca agatgcgctt gtaattgtat
    15241 gtgatactgc caatgctcca cgaattgacg atgaacgtta tagtacaggt cgtaaactta
    15301 ttaaaataga tcatcatcct gcagttgatc agtatggtga tattaattta gttaatacga
    15361 acgcgtcatc tacaagtgaa atcatttacg atttaatctc acattttaat gatgaagcaa
    15421 ttgttaataa agatatcgcg agcgttttat atcttggtat cgtcggtgat actgggcgat
    15481 tcctttttaa caatacctca gaacatacta tggaaattgc tggaaagtta attgggcatg
    15541 atattgatca taatgcgctt ttaaataaaa tgatggagaa ggacccaaaa atgttgccgt
    15601 ttcaaggtta tgttttacaa catttcgaac ttatggatga tggattctgc caagttaaaa
    15661 taactgaaga tgtattggag caattcggta ttcagccaaa tgaagcatct cagtttgtta
    15721 atacaattgc tgacatcaaa ggcttgaaaa tatgggtatt tgcagtcgat gaaggtaatg
    15781 aaatcagatg tcgattacgt tctaaagggc aattgattat taatgatatt gcgcaagatt
    15841 ttggtggcgg tggtcatccg aatgcgtcag gagtttcagt ggacagctgg gatgaatttg
    15901 agcaacttgc tacagcttta cgcacaaaac ttaactaata gaaaggggcc attcaatcat
    15961 ggtggcatat ttaaatattc atacggctta tgatttgtta aattcaagct taaaaataga
    16021 agatgccgta agacttgctg tgtctgaaaa tgttgatgca cttgccataa ctgacaccaa
    16081 tgtattgtat ggttttccta aattttatga tgcatgtata gcaaataaca ttaaaccgat
    16141 ttttggtatg acaatatatg tgacaaatgg attaaataca gtcgaaacag ttgttctagc
    16201 taaaaataat gatggattaa aagatttgta tcaactatca tcggaaataa aaatgaatgc
    16261 attagaacat gtgtcgtttg aattattaaa acgattttct aacaatatga ttatcatttt
    16321 taaaaaagtc ggtgatcaac atcgtgatat tgtacaagtg tttgaaaccc ataatgacac
    16381 atatatggac caccttagta tttcgattca aggtagaaaa catgtttgga ttcaaaatgt
    16441 ttgttaccaa acacgtcaag atgccgatac gatttctgca ttagcagcta ttagagacaa
    16501 tacaaaatta gacttaattc atgatcaaga agattttggt gcacattttt taactgaaaa
    16561 ggaaattaat caattagata ttaaccaaga atatttaacg caggttgatg ttatagctca
    16621 aaagtgtgat gcagaattaa aatatcatca atctctactt cctcaatatg agacacctaa
    16681 tgatgaatca gctaaaaaat atttgtggcg tgtcttagtt acacaattga aaaaattaga
    16741 acttaattat gacgtctatt tagagcgatt gaaatatgag tataaagtta ttactaatat
    16801 gggttttgaa gattatttct taatagtaag tgatttaatc cattatgcga aaacgaatga
    16861 tgtgatggta ggtcctggtc gtggttcttc agctggctca ctggtcagtt atttattggg
    16921 aattacaacg attgatccta ttaaattcaa tctattattt gaacgttttt taaacccaga
    16981 acgtgtaaca atgcctgata ttgatattga ctttgaagat acacgccgag aaagggtcat
    17041 tcagtacgtc caagaaaaat atggcgagct acatgtatct ggaattgtga ctttcggtca
    17101 tctgcttgca agagcagttg ctagagatgt tggaagaatt atggggtttg atgaagttac
    17161 attaaatgaa atttcaagtt taatcccaca taaattagga attacacttg atgaagcata
    17221 tcaaattgac gattttaaaa agtttgtaca tcgaaaccat cgacatgaac gctggttcag
    17281 tatttgtaaa aagttagaag gtttaccaag acatacatct acacatgcgg caggaattat
    17341 tattaatgac catccattat atgaatatgc ccctttaacg aaaggggata caggattatt
    17401 aacgcaatgg acaatgactg aagccgaacg tattgggtta ttaaaaatag attttctagg
    17461 gttgagaaac ttatcgatta ttcatcaaat cttaacacaa gtcaaaaaag atttaggtat
    17521 taatattgat atcgaaaaga ttccgtttga tgatcaaaaa gtgtttgaat tgttgtcgca
    17581 aggagatacg actggcatat tccaattaga gtctgacggt gtaagaagtg tattaaaaaa
    17641 attaaagccg gaacactttg aagatattgt tgctgtaact tctttgtata gaccaggtcc
    17701 aatggaagaa attccaactt acattacaag aagacatgat ccaagcaaag ttcaatattt
    17761 acatccgcat ttagaaccta tattaaaaaa tacttacggt gttattattt atcaagagca
    17821 aattatgcaa atagcgagca catttgcaaa cttcagttat ggtgaagcgg atattttaag
    17881 aagagcaatg agtaaaaaaa atagagctgt tcttgaaagt gagcgtcaac attttataga
    17941 aggtgcaaag caaaatggtt atcacgaaga cattagtaag caaatatttg atttgattct
    18001 gaaatttgct gattatggtt ttcctagagc acatgctgtc agctattcta aaattgcata
    18061 cattatgagc tttttaaaag tccattatcc aaattatttt tacgcaaata ttttaagtaa
    18121 tgttattgga agtgagaaga aaactgctca aatgatagaa gaagcaaaaa aacaaggtat
    18181 cactatattg ccaccgaaca ttaacgaaag tcattggttt tataaacctt cccaagaagg
    18241 catttattta tcaattggta caattaaagg tgttggttat caaagtgtga aagtgattgt
    18301 tgatgaacgt tatcagaacg gcaaatttaa agatttcttt gattttgcta gacgtatacc
    18361 gaagagagtc aaaacgagaa agttacttga agcactgatt ttagtgggag cgtttgatgc
    18421 ttttggtaaa acacgttcaa cgttgttgca agctattgat caagtgttgg atggcgattt
    18481 aaacattgaa caagatggtt ttttatttga tattttaacg ccaaaacaga tgtatgaaga
    18541 taaagaagaa ttgcctgatg cacttattag tcagtacgaa aaagaatatt taggatttta
    18601 tgtttcgcaa cacccagtag ataaaaagtt tgttgccaaa caatatttaa cgatatttaa
    18661 attgagtaac gcgcagaatt ataaacctat attagtacag tttgataaag ttaaacaaat
    18721 tcgaactaaa aatggtcaaa atatggcatt cgtcacatta aatgatggca ttgaaacttt
    18781 agatggtgtg attttcccta atcagtttaa aaagtacgaa gagttgttat cacataatga
    18841 cttgtttata gttagcggga aatttgacca tagaaagcaa caacgtcaac taattataaa
    18901 tgagattcag acattagcca cttttgaaga acaaaaatta gcatttgcca aacaaattat
    18961 aattagaaat aaatcacaaa tagatatgtt tgaagagatg attaaagcta cgaaagagaa
    19021 tgctaatgat gttgtgttat ccttttatga tgaaacgatt aaacaaatga ctactttagg
    19081 ctatattaat caaaaagata gtatgtttaa taattttata caatccttta accctagtga
    19141 tattaggctt atataacttt tatcaactaa taaattatga tatagtaaac tgatggttag
    19201 atatttttta accatcagtt tgcgtttata ttagtttttt atgcttatta tttttatgag
    19261 tttcacttta cattattgat taatccataa gaataattag cacaaaaagc agtatacata
    19321 aattgagtaa agaattttgt cgatatatcg tgcggaaaaa taataacttt ttgatatatt
    19381 ggtttttgct atccattata ataattaaag
//
LOCUS       DALAAR010000050        18242 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000050 DALAAR010000000
VERSION     DALAAR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.052"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(98..856)
                     /locus_tag="O2458_001845"
     CDS             complement(98..856)
                     /locus_tag="O2458_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ3887708.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            complement(998..2566)
                     /locus_tag="O2458_001846"
     CDS             complement(998..2566)
                     /locus_tag="O2458_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ3887709.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            2908..3993
                     /locus_tag="O2458_001847"
     CDS             2908..3993
                     /locus_tag="O2458_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ3887710.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(4188..4958)
                     /gene="fabI"
                     /locus_tag="O2458_001848"
     CDS             complement(4188..4958)
                     /gene="fabI"
                     /locus_tag="O2458_001848"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCZ3887711.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(5236..7080)
                     /locus_tag="O2458_001849"
     CDS             complement(5236..7080)
                     /locus_tag="O2458_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCZ3887712.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(7090..8475)
                     /gene="mgtE"
                     /locus_tag="O2458_001850"
     CDS             complement(7090..8475)
                     /gene="mgtE"
                     /locus_tag="O2458_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCZ3887713.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(8496..9350)
                     /locus_tag="O2458_001851"
     CDS             complement(8496..9350)
                     /locus_tag="O2458_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ3887714.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(9347..10156)
                     /locus_tag="O2458_001852"
     CDS             complement(9347..10156)
                     /locus_tag="O2458_001852"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCZ3887715.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(10173..10808)
                     /locus_tag="O2458_001853"
     CDS             complement(10173..10808)
                     /locus_tag="O2458_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ3887716.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(10825..11172)
                     /locus_tag="O2458_001854"
     CDS             complement(10825..11172)
                     /locus_tag="O2458_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887717.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            11358..11951
                     /locus_tag="O2458_001855"
     CDS             11358..11951
                     /locus_tag="O2458_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCZ3887718.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            12055..12420
                     /locus_tag="O2458_001856"
     CDS             12055..12420
                     /locus_tag="O2458_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCZ3887719.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            12443..13249
                     /gene="yjbH"
                     /locus_tag="O2458_001857"
     CDS             12443..13249
                     /gene="yjbH"
                     /locus_tag="O2458_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCZ3887720.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(13709..15517)
                     /gene="pepF"
                     /locus_tag="O2458_001858"
     CDS             complement(13709..15517)
                     /gene="pepF"
                     /locus_tag="O2458_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ3887721.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(15565..16550)
                     /locus_tag="O2458_001859"
                     /pseudo
     CDS             complement(15565..16550)
                     /locus_tag="O2458_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(16671..17390)
                     /gene="mecA"
                     /locus_tag="O2458_001860"
     CDS             complement(16671..17390)
                     /gene="mecA"
                     /locus_tag="O2458_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCZ3887722.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(17761..18156)
                     /gene="spxA"
                     /locus_tag="O2458_001861"
     CDS             complement(17761..18156)
                     /gene="spxA"
                     /locus_tag="O2458_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCZ3887723.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT         6156 a         3237 c         2487 g         6362 t
ORIGIN      
        1 gtcaaattta aattaaatta aacagtaaaa tcttttataa attatattat agaccagact
       61 atatgaagac aattcatact aaatctacta atgtaaatta ttcataaagt tgatcatctg
      121 tgtttttact taaaaaatac aataatatat ctgacagcaa tggtttccaa tctttccatt
      181 ggtgaccacc atcaaattca tcgtaataat aagttatatt atatttctta atttgttcag
      241 ctaattctct attcggtgtt aagaaatcgg cacgcttacc atttgttggt aaagtaaaat
      301 ctttttcatc tagaccaatg acatgccaaa ttgtcaattg ttctttattt gcacattgat
      361 ttagcttatc taatactttt tcatctgaat gtggacttaa cattgctaca cgactaaaaa
      421 tcgttggata ggtcaacgcc gttaataagg caatactacc cgctaaacta tcccctacta
      481 ataaccttgc attacctact ttcagtgtag aaaacgtcga gtcaataaat ggcaatattt
      541 ctttaccgac tgattgaata gttaaatgag aacgacttcc ttgtggatga aattcctctc
      601 tacgcttatc aacgtcttca taatggaatc caacaatgat cgcatcatca atacgcgctt
      661 ctttgattaa cgattcatat gtacgttgta ttctcccgaa acgtaaaaaa tctaatccgt
      721 caaagcaaag aatgacatta tatttaacaa gttgattata agattctggt aaataaatac
      781 ttaacgtgac atctctattt aaaatattac tatataaaac atgtttatta atcttaccag
      841 ttttaaattc tgacataaaa aagtgcccct agtagttgta ttgttattgt accaagagac
      901 actatatttt taaaattcaa atttctgagc acctaaaatt ctcaatactt ttctttttta
      961 tcaaatgatc actacatata ctataaacaa aagtagatta ctttttggca cgtgcttgtt
     1021 ttaaacgttc tggatacgtc ttcaaccaaa agacagcatt aggtaattta ttcggatcag
     1081 gttgataaac tgcattataa ccgttgccta aacgtttctt ttgaatttca taatctttta
     1141 aagcatttac ggcaggctta tgtaaaatcc aaatcgcaat gatatttaac caagccatta
     1201 gacctacacc taaatcaccg aatgcccatg ctacatcagc tgttttaact gcaccgtaaa
     1261 atgtagcgaa caaaataatc acacgagcaa tattaataaa tatcgatgaa acttgattat
     1321 tttgattacg cgttaaataa gcaacatttg tttctgtaat gtagtagtac gacaaaattg
     1381 tagtaaatgc aaagaagaat aaagcaaatg caataaagta cgaacctacg ccagagaaag
     1441 taggatcaaa ttgataacca ctgccatgga acgctttatc aatgccggct tgtgcataca
     1501 tcgcagtacc tgaataatct ttacctgttg cattttcaac ataaataccg ccatctttaa
     1561 ttaaatgcgg tgtgccattc gcattaaccg taccatcagt cacattatat gtaccagaaa
     1621 taagtataat cagagcagtt gcagtacata caaataatgt atcaatataa actgaaaatg
     1681 cttgtactag accttgttta cttggatgtg atacttctgc tgccgctgct gcgtgtggac
     1741 ctgtaccttg accagcctca tttgaatata atccacgttt aacaccaatc tctatcattg
     1801 cgccaacgat accaccaaaa gcagattgta atccaaatgc tgatttgaaa attaatgcaa
     1861 ataacgctgg cacttcttgt atattgatac aaatgataat gacagccatc agtatgtaaa
     1921 taattgccat aaatggtaca acggctgttg caacattggc aatactacgt acaccaccaa
     1981 aaataattaa tcccaaaata actacaacaa taccacccat taaccattgt ggaacatgaa
     2041 tcgcattatg catagaactt gctatagcgt tagattgcac accaggaagc aatagaccta
     2101 ctgagataat cgtaacaata gcaaagataa ttccgtaaat tttaccaaat ttaccaccaa
     2161 taccatattc aatataatac gctggtccac cacggtattc attattttca actctcttga
     2221 atatttgacc aagtgtagat tcaataaaag cactacttgc acctaaaaac gcagtaatcc
     2281 acatccaaaa tactgcacca ggtcctccta taaatattgc agtagataca ccgacaatat
     2341 tacctgtacc aaccctgcct gctaaagaca tggctatcgc ttgaaaactt gaaataccat
     2401 taggagactt ctctccttga aacattaatc gaatcatttc tttaaaatgt ctaacttgaa
     2461 aaaatcgcat acgcaatgta aatagcacac cagtaattag caaaccataa actaatggct
     2521 tactccaaac aacctcattt aaaaaggtta ctaatttttc aatcataaga ttccccctat
     2581 tgtttaaaca taaaaatatt atacgattaa gcacgagaca cttcaatata tttttcagaa
     2641 tattcttata aatattaata tgatcatttc actatttaaa cacgtttaat attaaaataa
     2701 gttattcata taaactggtg ctgtttgatc caattgcaga cttacgagtc attgaaaagt
     2761 ctcacaaact attgaaagta aatatcttaa aatagaaagt gaatggttaa tttaagtata
     2821 tttaaaaaat attaaccttt ttaagcactg ctatttagga tatactaaat aataactaag
     2881 tttagaaaaa taggaggaac atcgtttatg ttaaacaagg tttggttccg aactggcatc
     2941 gctctgatta tgctgttcat tctcatcaaa ctatttatgg aagtgcacga agtatttact
     3001 ccaatagcta caatcatcgg ttctgtattt cttccatttt taattagtgg ttttttattt
     3061 tatatctgtc taccttttca aaacttactt gaaaaagtcg gttttccaag atgggctagt
     3121 ataacaacaa tcatgttagc tttgtttgct attatcggct taattgttgc gtttgttgca
     3181 ccaatcatca tttcaaatat caacaattta attagtcaga cacctgccct acaaaaagaa
     3241 gcagagcaaa ttattaaatt cgcactagct caaatggata aattacctga agatgtaaca
     3301 agtagaatta caaatatggt taaatcaatg ggtgatggcg ttactaatat tttatctaat
     3361 tcattacagt acattacatc attaatttca accatcttct tattgattat ggttccattc
     3421 ttcttaattt atatgttaaa agaccatgaa aaatttatcc cagctgttgc taaatttttc
     3481 aaaggtgaac gtaaagtatt tttcgtagat ttattaactg atttaaactt tacattaaaa
     3541 tcttacattc aaggtcaagt aactgtcagt gttattttag gtatattctt atatatcggt
     3601 tattcaatta ttgacttacc atatattcct ttattagtac tatttgctgg tgtcgcaaac
     3661 ttaattcctt tcttaggttc atggttatca tttgcacctg cagcaatact cggtattata
     3721 gatagtccta ctacatttat atgggtttgt atcattacat taattgcaca tcaacttgaa
     3781 ggtaatatca ttacaccaaa tgtaatgggt aaatctttaa gtatccatcc tttaacaatt
     3841 atcgttgtta ttttagcagc aggagattta ggtggcttta cattagttct gattgcagtg
     3901 ccattatatg ctgtacttaa aacggttgtt agtaatattt tcaaataccg ccaacgcatt
     3961 attgataaag caaacagtaa tgttaaggac taattctgtg gatgtctttt aagaatataa
     4021 gatactatcg catcaaaagt tgaaactaca gcttttgagg cgattttttt gtgcataaaa
     4081 aatcagtcaa atgaaatatc aaataatttt ccatcagtcc gattattata aaagcaaaaa
     4141 agctttgctc acatatataa taacgtgaac aaagctgttg aatgatatta tttaattgcg
     4201 tggaatccgc tatctacatg aatattttca cctgtaacgc cacttgataa atcacttaat
     4261 aagtaagccg cagttttacc tacttctact tgatcaacat tacgttttaa aggtgcacgc
     4321 tcttcgattt ctttaagaat tgtattgaaa ccacccacac cttttgcact taatgtacgg
     4381 attggaccag ctgaaattgc attaacgcga atattatctg gacctaagtc taatgctaaa
     4441 tatttaacat ttgcttctaa gctcgcttta gcaacaccca tcacattata gttttgaact
     4501 gcgaattcgc cacctaaata tgttgttgca acaatgctac caccttctgg cattaatttt
     4561 ttagcttcat gagccacaat tgttaatgag taagaactaa tgtcttgagc taacaagaag
     4621 ccttcacgtg aagtttcaga aaagcgtccg cgtaagtctt ccatattagc aaatgcgatt
     4681 gaatgatata caccatcaat attgccaaca tctttaccaa tttgctcaaa accattaata
     4741 acctcttcat cgctttgaac atcaatttga tataagtgcg cttctggttg atttaattgt
     4801 tctaataatt tttcaagctc tttacggcta cgttctttac ggtaagtaaa tactaattta
     4861 gcacctaatt gatctaaaac tttagcgaca ccaaaagcaa tactacgctt attagcgatt
     4921 cccatgatga catatgtttt gttttcaaga tttaacatgt aagataactc cttataataa
     4981 ttaatttaac ggatagaata tctaatccca gttttaacta actttttatt cgtcaataca
     5041 ttttaacact attatgactt tttaataaag ttatttaaac gtattacaac gataaatgtt
     5101 tgatttagtc ctttctgtat caaacttgat ttacgtaaaa agcatttatt tatcaaaatc
     5161 atatacatac tagcatttta aaatcattaa ttttacgtcg tactaaaata cgtctgcatt
     5221 ttaatatcat tattgttaaa aataaaattc taactcttgc ttcaattcat caacgtattc
     5281 tttagcaccg gtaacaatta agcgatctcc atatcgcaat tgtgtatctc catgcggaac
     5341 aatcgactca ttattacgga taatacgcac gaagatgatg tctcctccga atgggaaatt
     5401 acgtaattga atattttcat atttataatt taacatttga atttcatata gtgacgtttc
     5461 aacattactt aataaattca acatgttagg tgtttcaatt aaacctttta ataagatttt
     5521 attacttaag tagctactga aaatttcaat acctgaatca actaactctg tatcgtccgt
     5581 tgtgctttca agtctgcaaa tgacacgctc aacttgatgt gctttggcta atttagcaac
     5641 ttttcggtta atatcatcgt cattcgtagc acaaacaact atgtctcggt caaacagacc
     5701 tagtcgttct aaaacatctt gttcataatc agcaatttct atcatcgtga tatcatctga
     5761 caattgacga cgatcactca agtctttgcg ataatataat gtcacgtcat ataactgaga
     5821 tgttaaattt tgcgctatag gaatcgttaa ttgattttta ccaattaaac taacttcaat
     5881 tttacggtta aactcatctg gaactggaaa cagttttttg aaaataatcg gaacgaatac
     5941 acatgtaatg actgcgctta aaattaatat acctgacgtt tcagctgaaa tagcatttaa
     6001 tctttctgaa attttggctg cagctatcac gagcgataat gttgatgtta ataaaaatgc
     6061 tgatgcaatc gttgttttca tatcaaacca acgtcgaata aacatgactg gaattaattt
     6121 tgaaatgata aatgcaacga ttaaaatcgg tatgataatt agtaatttcg gttctttaat
     6181 taatgaaggt atgtttaaat ctacaccaac cattataaag aaaataggaa taaagaaccc
     6241 ataaccaaat gagtctaact tttcaaccat ttcttcatct ggatttaata atgaaacaac
     6301 gacacctgct aagaatgcac ctaatatatt ttctgcgcca actccctctg ctagggctac
     6361 taataatatt attaatgcaa ataccgcacg aataccgatt tgcgtcgtac catccatcaa
     6421 tttttgtaaa aatgacattc ttttaaattg aacacctaaa atatatgaaa ttgctgtgaa
     6481 aacaactaat atacctatta accatattgt actgccgcct tgaccattga ttgcgccata
     6541 gaccgttaat aaaatcatag ttactaagtc cgcaagtact gctactaata ggataaattg
     6601 ccctatagtt gttctcataa tattcatttc ttttaaagtt ggaacaacta cgcctaagga
     6661 aatagttgaa atgataatga ccattaataa cacatcatcc actaatccaa gccatttaaa
     6721 tacatacgct aaaagaatcg aaataatcat aataaatgca aatacagtta acgctagatt
     6781 aagatgccct ggaattgagg attcatcatc attttgtcct tgacgtgcgc gtttatcttt
     6841 tttaaaagct ttaaaatcaa tttctaaacc acttaaaaac attaaaaaga taaagcctaa
     6901 cgttgataaa atatttagaa ttgaatccct ttctactata tttagaaatg aatttccaat
     6961 cacaatcccc atcaaaattt ctgcaacaac aactggcaag aaattaatat ttaatcgatt
     7021 gacaattatt ggagttaaaa acgctgctaa aacaacaata actaaagata aaaactccat
     7081 aactcctcct taaattaagt aagccataaa tgatgtagct aaaccaaaat aaatcaacat
     7141 actaataata tcattaattg ttgtaataaa tggtccacta gccactgctg gatcgatatt
     7201 taatttattc atcaataatg gaatcatcga acctacaaac gtccccaccg tcatcgcaca
     7261 agttaaactt cctgcaacga ttaatgctaa aagtggctga tgatatattg caacaataat
     7321 tgtaaataat attgttgaac atacaacacc cgataataca ccacttcctg cttctcttaa
     7381 tgcaattcta aatttacttt gctcattaat ttcccctgtc gtaatgttac gaactgaaac
     7441 ggctaaagat tgtgtacctg aatttcctga cataccacta ataataggaa taaacgctgc
     7501 gagtagcgct acattttcta atgttttttc gaatctccct aaaattgtcg cagtaatcat
     7561 tcctaaaaat gttaaaataa tcaaccatgg taaacgtttt aatgctgttt taatgataga
     7621 atcattagtc gaatcgatat ctgatacccc ggctaaacga gagtagtctt cactagcctc
     7681 ttcatccata acgtctaaaa tatcatcaat cgtgatgata ccaagcaaat gttcttggta
     7741 atctataaca ggtacagcca tgaaatcata gtctctcata acttgagcaa catcttcttg
     7801 gtcgtctgct acattcacac taatgacacg ttcattcata atatcttcaa tataagcatc
     7861 attttcagct acaattaaat ctcttagcga taaaacacct actaatttac catcatcatc
     7921 aacgacaaat ataacatata ttgtttctgc gtctggtgct tgcgctttga ccaataataa
     7981 tgcttcttta acaggcgtat gcgctttaag tgataaatac tccgtcgtca taataccgcc
     8041 ggccgtatcc tcatcatagt gaagtaatgc tttgatttca ttcgcgtcat ctttattcat
     8101 caatgttaaa agactagcaa cttttggttt agtcaactca tttaaaatat ctactgcatt
     8161 gtcgtatgac atttcttcta atatgtgact tgcgtatgtc gcattcatct tatcaaatag
     8221 caactcatat tcatcgtcat caatatctaa ttgatcaaag aaatttgcaa cttcttcagg
     8281 tgataaatat tgaaaaatct tttgtctatt ttcatcggta gtatcttcaa aatactcact
     8341 ttgttcatat gtgtgtaatg ctagaaattc atctctaaat tgatcaatat catcattttc
     8401 taaatattga tctaataaag tttggtcgta taattcctct tgaacacgct ctttttcatc
     8461 tgtgttcatt gacatactta caccccctgt gtgaattaga gttgattgaa taatttaagt
     8521 aattgcttat aatcaattgt aatataaata ttgttcttat tgattggatg aacaaaatat
     8581 atttgcgtac attgcagtac ttgaccatga attttgtcat gaaaaccatc atacaaagaa
     8641 tctcccacaa ttggatgccc aatatgttga aaatgtacac gaatttgatg tgtacgtccc
     8701 gtatgcaaat gaactttgca taaactgtat ttatcattct gatttattac ttcataagat
     8761 gttttagcgt atttaccatc cgaggcaact tctctagtta taatcctatc ctttgaccgt
     8821 ctaatattag cttcaataat accagatgta tgggttttac catatacaag gcaagtatat
     8881 atttttttca agtttacttt agaaaataaa tgatggatat gtccatattt agcgaatatc
     8941 acaataccag ttgtattacg atctagacgc gtaacaatat gtgggttaat attttcacca
     9001 tgttcctgac aatgatatag tacttgttcg attaaacttt catgaggatg ttctctcgaa
     9061 ggcgtacaat tttgattgtt tggtttagta actatgatga taaaagcatc ttcatacaat
     9121 acttctagct tacgagcata aggtattaaa ttaacactcg gtatttctcg cggtaaatga
     9181 atttctaata tatcatttgt cattaattgc ttacgcactg tgactggttc atcattaaca
     9241 attaaagcgc cattattttt aatggcgctc actgtcttct tagaaaaatc atgtcgtgct
     9301 aaaaaagttt taacagtttc ttgttgtgat atatgatact taaatttcat cgttcttcat
     9361 cacttgatat gaaagaatcg tgcacacgtt tccagaatgg gaatggtcta aaacgtgcaa
     9421 acctcacttt ttcatttgct acacggtatt gtattgaatt aacattttta tgtttgatac
     9481 taacatgatc tatcgtcatt ctaatggtat catgattaac tggtgatatt aaacatgtat
     9541 gatgctttgg taatacaagt ggtgatccta ccgttctaaa cacacgatta tttatcgagg
     9601 caatttctgt aatttgcatt gcttcaagtg aaggatgtat cagtgcgcca cctagcgctt
     9661 tgttataagc cgttgaaccc gaaggtgttg atacacataa tccatcgcct ctaaatcgct
     9721 caaagtgttt ccctcttaag ttaacatcca caacaagtgt tgagccattt tcagttttca
     9781 tcgttgcttc atttaatgct aaataccttg tttcatagcc gttgtcgttg tatctcataa
     9841 taatttcaag caatggatat tcaatgacct gaaactctga attattaatt tcgatgatta
     9901 atttttcaac ttcatgaggt aaccaatccg cataaaatcc taaatgacct gtatgaactc
     9961 caacaaatgc cacttttgat aacatgtggc tatactgatg gaatgcttgt agtaatgtac
    10021 cgtcaccacc aactgaaata acaatttcag gattttcact atcctcaatc atgcgaaaat
    10081 ctttcatata gttcatcatt ttatgcttta aggcattaga cttggagtca cctttagtta
    10141 aaattgtata acgcatgatt tataacacct cgttaatcat tttcatgttt tttagaacgt
    10201 ttttgcgtgt aatatttttg agcttcctga atttcatctt taatttcaga catctcttca
    10261 tcaagtaaat acgctgcttc tgccgctctt tccaaacgat gttgaatttc atccggataa
    10321 gcaccatcat atttatatcg taaagtatgt tcaatcgttg cccagaaatt cattgctaat
    10381 gtacgaatct gaatctcagc caatataaat ttttggcctt gtaatgtttc aattggatat
    10441 tcaataatga catgatacga gcggtaacca ctttctttag tgttacgaat ataatctcgt
    10501 tcttcaatta ctttaaaatc ttttctttgt cttaaaatat tgacgacaac atcaatatct
    10561 tcaacaaatt ggcacatcat tcttaaacca gcgatatcgt acatttcttc tcttaaccta
    10621 tcaaatggta tttgtcgttt gtttgcctta tctataatac tagcgattgg tttaacacga
    10681 ccagtaacaa attctattgg cgacgcttgt tcaccaactt catattgttt gcgcatgcct
    10741 ttaagtttca ctttcaactc atcaaccgct tgcttataag gtgttaagaa ctgatcccat
    10801 tgattcatag tgcttcaccc cgctttattc aatgtcgaat gtttcttcaa cggttgcaac
    10861 aaattcctta ccataattat tattcccttt tatgttgtct aaaatataag taagttcttg
    10921 gtggaattca gtcaattcta gttcatcatt gataataatt tttttagtca tgttttcatg
    10981 caacatcgtc catgtttctt gtacgaaaat gagataagca tacgctttac catcatctaa
    11041 tgtatacgca aaggcataat tatcactatc aacgagcatt tgtcctactt cagataatcc
    11101 tttaatagaa tcttctgtat aacaataaag tatgtcatct ttaattttaa tttcattaat
    11161 atataaacgc atgcttaatc ctcctcttat tccataacat tttaacacaa ttcaataata
    11221 caaaacactt acgaacacta tttttaatgg tcaaattaac attgataata gataaaggaa
    11281 attactttta aaattcaact ttgtcattca taatatagtt aagataaatt gaatttgata
    11341 cgaggtgtat acatatcatg gcaacaaatc atgaaataga atttaaacaa atgataactg
    11401 catcaatcta taataaattg caagagaaat attttaaaga cagtgtgctt tttaagcaag
    11461 tcaattacta tattgataca cccgatttca aattaaagga acatcgctct gcactacgca
    11521 taagagttaa agacaatcaa tatgaaatga ctttaaagac accagcaaag gttggtctat
    11581 tagaatacaa ctatatcgtc gacattaaac cagagatgaa cttgattatt tcaaacgaca
    11641 atcttccaga tgacattcgt caaattatag ttgaacagtt tggtgtaaaa gatacaactt
    11701 tatcaatact tggtgcatta acgacatatc gccaagaaac taaatataaa ggtgatttac
    11761 tggttttaga taaaagtgag tatttcgata ctacggatta tgagttagag ttcgaagtta
    11821 aagactataa tcaaggttta caaaagttcc aatctttatt aaacgaacta aacctcgaac
    11881 atcatcaacc tttaaataaa gttcaacgtt ttttcaagaa aaaagaaaca ctttcaaaca
    11941 atataaatta aattggcaaa tatgcatatt tctttcaaat gtttaaaatc atgttatatt
    12001 agttatatat taacttaagt tcgtaacagt aaatttttga aaatgggtga ttaaatgaca
    12061 acaacaccat atgacatcat tggtaaagaa gcgttatacg atatgattca ttatttttac
    12121 acccttgtag aaaaagatga acgacttaat cacctgtttc caggagattt tgcagaaaca
    12181 agtcgtaaac aaaaacaatt tttaacacag tttttgggcg gtccaaacat ttataccgaa
    12241 gaacacggac atcctatgct aagaaaaaga catatagatt ttacaatcac tgaatttgaa
    12301 agagatgcat ggttagaaaa tatgcagaca gcaattaatc gcgctgcgtt tccacaaggt
    12361 gttggagatt atttatttga gcgcttaaga ttaactgcta atcatatggt gaattcctaa
    12421 aattaattgt aggtgaataa acatggctgg agaattacga ataatggaaa ataagagtcg
    12481 tgaagatatc aatctatcac ctgtaagtaa aatcgaaatt tattcatttt tcgatccatt
    12541 tagctccgat tgcttcaaat tatcagcaat cttatccaaa ttaagaattg aatataatca
    12601 atatatacgt atcagacata tattaaatcc ttcgttaaaa gtattaacga aatgccaagc
    12661 tcaaagtaca tccaactttg ataacatcgc cctagcttat aaagcagctg agttacaagg
    12721 tcgtgtacgt gccgaacgat ttatacattt aatgcaaaat gaaatcatac ctaaaagaga
    12781 tattattaca gaatcaatga tttgtgactg tattcaaaat gcaggtattg atttagaagt
    12841 atttaaagac gacttacaaa aaagtaaact aaccgaaagc ttgaaaattg atttgcatat
    12901 tgcaagagaa atggaaatcg aacaagcccc ttctctcgtt ttctttagtg aagatgttca
    12961 tgaagaaggt ttaaaagtcg aaggattata cccatatcac atctatactt atataattaa
    13021 tgaattgatg ggtaaaccta tcgaaaagaa tcttcctcct aaattagaaa cttatataca
    13081 gcaacaacaa cttgtaacga tggaagaatt acttactatt tatgaatggc cagaaaaact
    13141 tttaaacaaa gagttaaaga agttagccat tcaacaaaaa attgaaaaac ttaaatatcc
    13201 agatggcgat ttctggaaat ctaaaatgcc taaaatcaaa tcaaaataaa taaattaaag
    13261 acctcgtaca ccataaatgt aacgaggtct ttttactgac tcttaatttt gcaacattaa
    13321 attattagaa cgtaatgtta cacatcgatc taaatatatt agtgatattt ttcgaattta
    13381 accagttcga aaattataca aattataaat ttggtacata atagacatat atgaatcgtt
    13441 aatgtataaa aagtgcatat ttaatacatt tttatacaac ttcacttccc tcttattaaa
    13501 aaagaacata aaaaaacgtc gtgtctgaat acacgacgct aaacaaaggg gatgggagaa
    13561 atttttcact tcaaacaaag gggtatgttt gttatgtgat taatttcatg ttcataatat
    13621 aacatgctat ctctaccatt tcaacctttt aatagtactt aatttaaatt gtcacaatct
    13681 gttcataatg taagcgaata caatagtact aagctttcat taatttttca aaagcgttca
    13741 atttttgttc aaaaacttca caagcttgtt caattggttc aggtgttgtc atatctacac
    13801 cagcattttt taatatctca attggataat ttgagctacc ttttttcaag aattcattaa
    13861 tatatctatc tactgctggc ttaccttctg ttaaaatttg atgacttaag ctttgagctg
    13921 cactgtaacc agttgcgtat tgatatacat aataattcat atagaagtgt ggaatacgtg
    13981 accattcctt actaatatct tcatcagttt ctacagaatc accgaagtat aatttattta
    14041 atttggcata ttcttcattc attctagttg gcgttaatgg ttcacctgct tcttcaattg
    14101 catgaatttt atgctcaaat tctgcgaaca ttgtttgtcg gaataatgta gctctgaaac
    14161 gttctaattc ttggtttaat aataataagc gtttttcatc atcaagatgt ttatccatat
    14221 aatcacttaa aagtgcttcg ttacaagttg atgcaacttc agcgacaaaa atagtgtagt
    14281 cactagaatt tgacggttgg aattttctac tgaagtaact atgtgctgaa tgcccaaatt
    14341 catgaactaa tgtgtataag tctgaaatag tattagacca gttaagtaga ataaatggat
    14401 tagttaaatg tgcacctgat gaatagccac ctgaacgttt acctttattc tcatagacat
    14461 cgacccaacg attgtttaag ccttctttaa ctacatttaa atattcttca cccattggtt
    14521 ctaatgcttt taacatccac tctttagctt cttcataagg catttcaaac ttaatatctt
    14581 taattaatgg tgtatataaa tcatacattt tcaagtcatc taaacctagc aattctttgc
    14641 gcaattcagt atatctatgt agcaatggta aatatttatg tacagttttt actagattgt
    14701 catatacatt ttctggaata tgattattac tcaatgcttt ttctcttgca gttttgtaat
    14761 tgtgtgtacg agcattaaat acatttttct tcacttcacc tgctagcgta gcgccaagcg
    14821 tattattatg agcaccatat gctttatata catttctaaa agcactttct cttagtttgc
    14881 gatcatctgt ttctaaatac ttaataaatg taccttgtgt taacgggtgt gcatttccat
    14941 ctttatctat cgcatcttca aatactaaat cagcgttgct aaacataccg tatacatttg
    15001 atggcgttga taacgcgtcc tgcgcttctg ttaataactt ttcagtttca gcatctaaaa
    15061 tatgaggacg tttttcatta atcaacttca aatcaaatgc gaatttttgt aatttatcat
    15121 atgaatttac aaatgattga attttatctt catcaatttg taaaatctct ggcactaaga
    15181 aactccatgc cgagctaaat ttaataatta attgatgtgc tcttgactcc ataccagtat
    15241 acttgtcgtt cgttgtatct tggtcttgtt ttaggtgcgc gtatacatat actttttcta
    15301 atttagtacc taatgtatct tctaattcta acgcattgta taatgtctca gcactatcac
    15361 caatgtgtcc tttaaattgt tcttctttgc ctaactcatt ttcaacttct ttaaatgcag
    15421 cctcaaaagc ttcatcatct ttgaaaattg ttgttaagtc ccatgtatat tcaggatatt
    15481 tacgttcctg ttcttctctc gataattgtt gactcatatg tagtaaacct cctaataaaa
    15541 tataattgat ataattttct cattttagaa caaaatttgc acgtcattac ctttatctga
    15601 acaaatattt aaataattgt taataagttt ttctataatt gctgttttac tttctagagc
    15661 aacaataaat tttttcagtt taaaataatt aagattgtca tttactaatt tgctttttcc
    15721 attctttaac cataaatcaa cttgtaattg ccactcaata ggatgatttt taatataaat
    15781 ttgctctgga aaaatataac cataattgtg cactacttct tgatcagtta actgtaattg
    15841 atacattgca cttaaagtgg gttctaaaac agaattttgc cagcgacaat atttgatata
    15901 ttgattaatt gcgaactttg ataatttata aacagcatga taatcacaag gcgcctcatt
    15961 aaacaactca ctaattttgg catttctttt ttcagcgaca tatttgtgcc cgcctaaata
    16021 ttgtatttgt tgaaacatca ttatttgttt tttctctaaa ttgaacgtat gaagttttcg
    16081 agtgaatgga ttaataaaca tactttgaaa atgattgaac ttcactttat tatgacaata
    16141 atcaacatca tttaataacc aacttacttt atatcccatt ttctttaaac cttccgttcg
    16201 ttgaagaatc tgtttataag gaattggcga atactgtagc tcaataacat atttattatt
    16261 aatcaaaata tccggaattt gttttatttc ttttaaaatg gctctatctc tactttacat
    16321 ccttgttgct gtatcatctg tgctaaaatc aatttacttt tataatgttt aatcgtttca
    16381 ttattaaaac atttttgttc cgctaaatgt ttatgtgcaa aatgactaat tactttgagc
    16441 ccacgcttta aaataacttg cttgccacac accggacaaa aatattgtgt ctttctcaat
    16501 gcagtagttg ctaatacgcg ttccttttct tcatttaaag ctactaacat atcttcacct
    16561 caaaatatta cacgtaagtt taactactat tactttttca gcttttgaaa tcaataaaaa
    16621 aagagactcc aaagagtctc ctttcatagt gcgttgctta attaatatcg ttattcagtt
    16681 gtctctggaa aataacgtcg aacttgagct gttacgttat gactcataat tattttagca
    16741 tagtcattta aataaacttc tgttctgtct gttggataag caaattcaag caattgactg
    16801 taactatcat taatgacttc ttgatcaaca tgactatcaa aatatacagc ataataataa
    16861 gtaccatcaa ccatatataa caaatcttca aactctgtag ttattggatt gctatgatat
    16921 gcataattaa taacatcttc taaatcgtta aatttcacaa tgattgttct tgtattttta
    16981 cgtgctgaag acttttgacg tttagaacct tgagcttctt tttcttttgt tcgttgctcg
    17041 aataattctt ctaattgatc ttcaccttct aatgtttgag ctaacaattc ttgaacttgt
    17101 tcatcaaatt gatcagttgc atcatcatca gacatattca tcatatcttc atttttagat
    17161 ttagaaattg tgacttcgac acctttttca aaggcatgta cttgaatcca taatggacct
    17221 tctacaacaa aatcttcttc ttcgttaatt tcatccatca ttgaccaaaa gaattcttcg
    17281 ccacgtttgc gatttgtcca taaatcttca cgactaaatc cacgggcctc gatatcgcta
    17341 tatgttataa acaattttac agttgtatca tctactcgtt ctattctcat atcatctcac
    17401 tccttacagt cgatgaatag tatccacatt gtattaaagc atcaaacaaa atacaattta
    17461 tttgtttgat ttctttctct aaaatatttt ggtaacttat aataacatat tcgttgtagc
    17521 aaaactattt aaatgattta tacagttaaa gcattaaagc actttcttaa tttaatttta
    17581 ccttatcata gcaaatgttt catccaaaac actctaatat agagctatat taacaattgc
    17641 acatttgatt taaaagatac taagcaatta ttgtaaaatt aaaaccaaaa aagcaacact
    17701 atagaaatag tgttgcttga catatctttt agtaaagtat gacacatagt taaatggtta
    17761 ttagtcaacc atacgttgtg cttcttgtaa ttggaacgta cgaacttttc taggtaagaa
    17821 acgtcgaatc tcgtcctcat tataaccaac ttgtagtcgt ttattatcta aaataattgg
    17881 acgacgtaat aagccaggat tatcttgaat gattgaatat aagtcttgta atggtagtga
    17941 atcaatatca acatttaatt tttggtatgt tttagaacgt gtagaaatga tttcatcagt
    18001 accgtcttca gtcattttta atatttgctt aatttcatca attgttaaat gttcagaaaa
    18061 aatattacgc tccgtatacg gaatgtcatg ttcttgtaac catgctttcg ctttacggca
    18121 agatgtgcaa cttggtgaag taaataatgt taccatacat ctcactctcc tatttgaatg
    18181 aataaaattc attgcttaaa atttagttat agatcaagaa aaaacatttt tttctaaaat
    18241 tc
//
LOCUS       DALAAR010000051        18104 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000051 DALAAR010000000
VERSION     DALAAR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.053"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            219..620
                     /locus_tag="O2458_001862"
     CDS             219..620
                     /locus_tag="O2458_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3887724.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(740..1303)
                     /locus_tag="O2458_001863"
     CDS             complement(740..1303)
                     /locus_tag="O2458_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3887725.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1300..2253)
                     /locus_tag="O2458_001864"
     CDS             complement(1300..2253)
                     /locus_tag="O2458_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCZ3887726.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2363..3544
                     /locus_tag="O2458_001865"
     CDS             2363..3544
                     /locus_tag="O2458_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887727.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3832..6249
                     /locus_tag="O2458_001866"
     CDS             3832..6249
                     /locus_tag="O2458_001866"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCZ3887728.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6271..6582
                     /locus_tag="O2458_001867"
     CDS             6271..6582
                     /locus_tag="O2458_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ3887729.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            6908..13468
                     /gene="sasC"
                     /locus_tag="O2458_001868"
     CDS             6908..13468
                     /gene="sasC"
                     /locus_tag="O2458_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCZ3887730.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(13585..14853)
                     /locus_tag="O2458_001869"
     CDS             complement(13585..14853)
                     /locus_tag="O2458_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ3887731.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            15260..16921
                     /locus_tag="O2458_001870"
     CDS             15260..16921
                     /locus_tag="O2458_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ3887732.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            16918..17613
                     /locus_tag="O2458_001871"
     CDS             16918..17613
                     /locus_tag="O2458_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ3887733.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            17630..18052
                     /locus_tag="O2458_001872"
     CDS             17630..18052
                     /locus_tag="O2458_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ3887734.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
BASE COUNT         6642 a         2770 c         3303 g         5389 t
ORIGIN      
        1 atgtactatt atcttatttc taaatattaa ctctattgat tattggtttt tatacttatt
       61 taattttatt caactttgac aattgaatag aaagcaagtt tatttacact tgtagtttta
      121 tgcataagtt agcacataca agttttggga ttgttgggat gtttgttaat acttgtatag
      181 tagctaaata tgtgattatt aattgggaga tgtttagcat gaaaaaagta aataacgaca
      241 ctgtatttgg aattttgcaa ttagaaacac ttttgggtga cattaactca attttcagcg
      301 agattgaaag cgaatacaaa atgtctagag aagaaatttt aattttacta actttatggc
      361 aaaaaggttc tatgacgctt aaagaaatgg acagatttgt tgaagttaaa ccgtataagc
      421 gtacgagaac gtataataat ttagttgaat tagaatggat ttacaaagag cgtcctgttg
      481 acgatgaaag aacagttatt attcatttca atgaaaagtt acaacaagag aaagtagagt
      541 tgttgaattt catcagtgat gcgattgcaa gtagagcaac agcaatgcaa aatagtttaa
      601 acgcaattat tgctgtgtaa gtttaatagc ataaaaagag gttttcatta agttgaaaac
      661 ctctttttgt tgttggcatt aatttttcaa atgttgacta ctcaatccta aattataaat
      721 agtatagcgc agcaaatgct taagaaattt tttctatggc acaaatgaat ggagcatgat
      781 tacgttggtt taaaaattga tattgcaaaa cttgcgcatg cttttgatcc aaagtactca
      841 agtaatcaag caatgcatgc ttctcaattt gtccttcgct atgaccatga tatataacaa
      901 gtacaataat accttcaatt gacattaatg atagcaatga attaatagct tggattgtcg
      961 tgtcaggctt tgtcacgata gatttatcac ctttaggcaa ataacctagg ttaaagatgg
     1021 ctgcatcaat atgaccttta tgtgcatcat ttatatgatg ttcaatattt tcatgtccat
     1081 cttttattaa agaaacatga ttgaaatcct taactttatc acgtgtattt tccaaagcta
     1141 aatcttgaat gtcgaaacca taaacatgtc cttctggtac ttgttcggct aaaaataaag
     1201 tgtcattgcc gttaccgcaa gttgcgtcta caacaatact ttctggtgtt atatgttgtt
     1261 taataagtgt ttttgaaaaa gggagtatac gttctaattt cattgcttca ccttagactt
     1321 gtaacgcaag ccttgataag aatttctacg tgctaattca gcatcgatgc catttaatac
     1381 ttcccattta ttaacactcc acattggacc taccatgata tctattggac catcaccggt
     1441 aattcggtga acgatcattt cagggggaat cacttctaat tggtccacaa ctaggtttgt
     1501 gtactcttct tgagtcataa aagttaataa acctttatcg tattgtttta ccatcggtgt
     1561 accttttaac aaatgaagta aatgaatttt aataccttgt acatccattt gtgcaacttc
     1621 tttggcagta gccatcatca tgtcatagtc ttcgccaggt aagccattaa tgatgtgtgt
     1681 acatacattg atattatgct tacgtaattt tgccacacca tcataataag ttttcatatc
     1741 atgggcacga ttgattaaat cagatgttga ctgatggatt gtttgtagtc ctaattcaac
     1801 ccataagtat gttcgttgat tcaaatctgc taaatattcg acaacatcgt ctggtagaca
     1861 gtcaggacgc gtaccaatag ataatcccac aacacccggt tctttaagta caggttcgaa
     1921 tttttctttt aatacttcaa ccggtgcatg tgtatttgta aatgcctgaa aataagcaat
     1981 atattttcct tcgtgccatt tctcatgcat cttttcttta atttctttaa attgtactgc
     2041 gattgaatct gcacgattac ctgcaaagtc tccgctacct gcagcagaac aaaatgtaca
     2101 tccaccatgt gctacagtgc catcgcggtt aggacagtca aacccgccat ccaatgcaac
     2161 tttaaatatt ttttgtccaa atttattttt taaatggtaa ttccatgtgt gataacgttt
     2221 gttttcaaaa gcgtattgga aatgattgcc catatgtcat tttcctttct ataaaaaaag
     2281 agttctaagt acagatttta acatatttta atgttatagt gtttattata gtttgacaaa
     2341 aaagagagag gaactatgaa atatgaatat acctaaatca gtctggtggc tagtaattgg
     2401 catggcgtta aatattactg gttccagttt tttgtggcct ttaaatacaa tttatatgaa
     2461 acaagaactt ggaaaaagtt taactgttgc tggtttagtg ctaatgataa attcatttgg
     2521 catggttatt ggaaacttat taggtggttc actatttgat aaattaggtg gatacaagac
     2581 gattttaatt ggaactttca cttgtctttg tagtacaacg ctacttaatt tctttcacgg
     2641 gtggccttgg tatgctgtat ggcttgtaat gttagggttt ggtggcggaa tgattattcc
     2701 tgcgatatac gctatggctg gagcagtgtg gccaaatggc ggaagacaaa cgtttaatgc
     2761 gatatactta gcgcaaaata ttggtgtggc tgtcggtgct gcaatgggcg gctttgtcgc
     2821 agaatttagc tttaactata tctttttagc caatcttatt atgtatgttg tgtttgcgct
     2881 tgtcgcggta acgcaattta atattgaaat taatgcgaaa gttaaatatc caactcattt
     2941 agatattact ggtaaaaaga ataaagcaag atttatttca ttagtactaa tttgtgcaat
     3001 gtttgcaatt tgttgggttg catatattca atgggagtct acaatcgctt catttacaca
     3061 atctattaat atttcaatgg cacaatatag tgttttatgg acaattaacg gaataatgat
     3121 tttagtagca caaccattaa ttaaaccgat tctctatctg ttaaaaggaa acttaaagaa
     3181 gcaaatgttt gtcggcatca tcatttttat gttgtcgttc tttgtcacga gttttgccga
     3241 aaactttaca atatttgttg tcggtatgat tattttaact tttggagaaa tgtttgtatg
     3301 gccagcagtt ccaactatag ccaatcagtt agcgccagat ggtaagcaag gacagtacca
     3361 aggttttgtg aattcagctg ctacagtagg aaaagcattt ggtccatttc ttggtggtgt
     3421 attagttgat gcgtttaata tgcgcatgat gtttatcggt atgatgctac tacttgtatt
     3481 tgcattaata ttattaatgg ttttcaagga gaataatacg caacctaaaa aaatagatgc
     3541 ataatgagta aatagaatta acgttataga cttgaaataa atgtcgttat aacataatat
     3601 taatttgtat aatttaattt cgtttggagc ttttctacag aaagctagtg atgctgagag
     3661 ctagtgttaa ggactaaatg taaatcgtat taattttaaa ttgaatgaat gacatctctt
     3721 actattaaaa tgagtgcaca atttttgtga aatagggtgg taacgcggca aatgtcgtcc
     3781 ctatgtaaat agaatagtta gaggtgtctt ttttattgaa taggaggaaa tgtgttgaat
     3841 tataaccaca atcaaattga aaagaaatgg caagactatt gggacgaaaa taaaacattt
     3901 aaaacaaatg ataacttagg tcaaaagaaa ttttatgctt tagacatgtt tccatatcca
     3961 tcaggtgctg gtttacatgt tggacatcct gagggctata cagcaacaga tatcatttca
     4021 agatataaaa gaatgcaagg atataatgta ttacatccga tggggtggga tgcattcgga
     4081 ttaccagcag agcaatatgc tttagacact ggcaacgacc cacgtgaatt tacaaagaaa
     4141 aatatccaaa cttttaaacg acaaattaaa gaattagggt tcagttatga ttgggatcgt
     4201 gaagttaata caacagatcc agaatactat aaatggacac agtggatttt catacagtta
     4261 tataacaaag gtttagcata cgttgatgaa gttgcagtta actggtgtcc agcattaggc
     4321 actgttttat ctaacgaaga agtgattgat ggtgtctctg aacgtggtgg acatccagtt
     4381 tatcgtaagc cgatgaaaca atgggtactt aaaatcacag aatatgcaga tcaattatta
     4441 gcagatttag atgatttaga ttggcctgag tctttaaaag atatgcagcg caattggatt
     4501 ggacgttctg aaggggccaa agtttcattt gatgtagata atacggaagg aaaagtagaa
     4561 gtatttacga ctagaccaga tacaatctat ggtgcatcat tcttagtctt aagtcctgaa
     4621 catgcattag ttaattcaat tacaacagat gaatataaag aaaaagtaaa agcttatcaa
     4681 acagaagctt ctaaaaagtc agatttagaa cgtacagatt tagcaaaaga taaatcaggt
     4741 gtatttactg gtgcatatgc aactaatcct ttatctggtg aaaaagtaca aatttggatt
     4801 gctgattatg tattatcaac atatggtact ggagcaatta tggcagtacc agcgcatgat
     4861 gacagagatt atgaatttgc taaaaagttt gatttgccaa tcattgaagt catcgaaggt
     4921 ggaaatgttg aagaagcagc atacactggt gaaggtaaac atattaattc tggtgaactt
     4981 gatggtttag aaaatgaagc ggcaattact aaagctattc aattattaga gcaaaaaggt
     5041 gctggcgaaa agaaagttaa ttacaaatta agagattggt tattcagtcg tcagcgttat
     5101 tggggcgaac caattcctgt cattcattgg gaagatggaa caatgacaac tgttcctgaa
     5161 gaagagctac cattgttgtt acctgaaaca gatgaaatca agccatcagg gactggtgag
     5221 tctccactag ctaatattga ttcatttgta aatgttgtag atgaaaaaac aggtatgaaa
     5281 ggacgtcgtg aaacaaatac aatgccacaa tgggcaggta gttgttggta ttatttacgt
     5341 tacatcgatc ctaaaaatga aaatatgtta gcagatcctg aaaaattaaa acattggtta
     5401 cctgttgatt tatatatcgg tggagtagaa catgcggttc ttcacttatt atatgcaaga
     5461 ttttggcata aagtccttta tgatttggct atcgtaccta ctaaagaacc tttccaaaaa
     5521 ttatttaacc aaggtatgat tttaggagaa ggtaatgaga agatgagtaa atctaaagga
     5581 aatgtaatca atcctgatga tatagtacag tctcatggtg cagatacttt gcgtctttac
     5641 gaaatgttta tgggaccttt agatgctgca attgcatgga gtgaaaaagg attagatggg
     5701 tctcgtcgat tcttagatcg cgtatggcgt ttaatggtaa atgaagatgg gacattgagt
     5761 tcaaaaattg taactacaaa taataaatct ttagataaag tttataacca aactgttaaa
     5821 aaggtaacag aagactttga aacattagga tttaatactg ctattagtca attaatggta
     5881 tttattaatg agtgttataa agttgatgaa gtttataaac cttacattga aggcttcgtt
     5941 aaaatgttag cacctattgc accacatatc ggtgaagaat tatggtcaaa attaggacat
     6001 gaagagtcta ttacgtacca accttggcca acttatgacg aagcactact tgtagatgat
     6061 gaagtagaaa tcgttgttca agtgaatggt aaattgagag ctaaaattaa aattgctaaa
     6121 gatacatcaa aagaagaaat gcaagaaatt gccttatcta atgacaatgt taaagcgagt
     6181 attgaaggta aagacatcat gaaagtcatc gctgttcctc aaaaattagt caatattgta
     6241 gctaaataat gttttaagga ggactttgaa atgaagtcaa ttactacaga tgaattaaaa
     6301 aataaacttt tagaatctaa accagttcaa attgttgatg ttcgtactga tgaagaaaca
     6361 gcaatgggat atattcctaa tgcaaagtta attccaatgg ataccattcc ggataattta
     6421 aattcattta ataaaaatga aatatattat attgtatgtg ctggtggagt tcgaagcgct
     6481 aaagttgtag aatatttaga ggcaaatggc attgatgccg taaatgtcga aggcggcatg
     6541 cacgcatggg gcgatgaagg tttggaaata aaaagtattt aaagtagtga cataatttaa
     6601 aataatatta catttgtaat gacaccaagt aacgtttcgg ttgcttggtg ttttttggta
     6661 tgaattactt tctgttacaa aacaatctaa agcgttcttg ttatgtttta ttaagatttt
     6721 aattacaaaa cggaaactaa attgtaataa aataaaactt tattttataa aatgatgatg
     6781 ataaaattga gtgaacttaa aatattgtac aaaataatat agctataaat ataatatgag
     6841 ggagcgtata tttttagcat aattcttaac aacacagcag agaacagaca accaggagga
     6901 aaatgaaatg aatttgttaa agaaaaataa atatagtatt aggaagtata aagtaggcat
     6961 attctctact ttaatcggaa cagttttatt actttcaaac ccaaatggtg cacaagcctt
     7021 aactacggat aataatgtac aaagcgatac taatcaagca acacctgtaa attcacaaga
     7081 taaagatgtt gctaataata gaggtttagc aaatagtgcg cagaatacac ctaatcaatc
     7141 tgcaacaacc aatcaagcaa cgaatcaagc attggttaat cataataatg gtagtatagt
     7201 aaatcaagct acgccaacat cagtgcaatc aagtacgcct tcagcacaaa acaataatca
     7261 tacagatggc aatacaacag caactgagac agtgtcaaac gctaataata atgatgtagt
     7321 gtcgaataat accgcattaa atgtaccaac taaaacaaat gaaaatggtt caggaggaca
     7381 tctaacttta aaggaaattc aagaagatgt tcgtcattct tcaaataaac cagagctagt
     7441 tgcaattgct gaaccagcat ctaatagacc gaaaaagaga agtagacgtg cggcaccggc
     7501 agatcctaat gcaactccag cagatccagc ggctgcagcg gtaggaaacg gtggtgcacc
     7561 agttgcaatt acagcgccat atacgccaac aactgatcct aatgccaata atgcaggaca
     7621 aaatgcacct aacgaagtgc tgtcatttga tgacaatggt attagaccaa gtaccaaccg
     7681 ttctgtgcca acagtaaacg ttgttaataa cttgccgggc ttcacactaa tcaatggtgg
     7741 caaagtaggg gtgtttagtc atgcaatggt aagaacgagc atgtttgatt caggagataa
     7801 taagaactat caagcacaag gaaatgtaat tgcattaggt cgtatacatg gaactgatac
     7861 gaatgaccat ggcgatttta atggtatcga gaaagcatta acagtaaatc cgaattctga
     7921 attaatcttt gaatttaata caatgactac taaaaacggt caaggcgcaa caaatgttat
     7981 tatcaaaaat gctgatacta atgatacgat tgctgaaaag actgttgaag gcggtccaac
     8041 tttgcgttta tttaaagtac ctgataatgt gagaaatctc aaaattcaat ttgtacctaa
     8101 aaatgacgca ataacagatg cgcgtggcat ttatcaacta aaagatggtt acaaatacta
     8161 tagctttgtt gactctatcg gacttcattc tgggtcacat gtttttgttg aaagacgaac
     8221 aatggatcca acagcaacaa ataataaaga gtttactgta acaacatcat taaagaataa
     8281 tggtaattct ggtgcttctc tagatacaaa tgactttgta tatcaagttc aattacctga
     8341 aggtgttgaa tatgtgaaca attcattgac taaagatttt ccaagtaaca attcaggcgt
     8401 tgatgttaat gatatgaatg ttacatatga tgcagcaaat cgtgtgataa caattaaaag
     8461 tactggagga ggtacagcaa actctccggc acgacttatg cctgataaaa tactcgattt
     8521 aagatataaa ttacgtgtaa ataatgtgcc gacaccaaga acagtaacat ttaacgagac
     8581 attaacgtat aaaacatata cacaagattt cattaattca gctgcagaaa gtcatactgt
     8641 aagtacaaat ccatatacta tcgatatcat catgaataaa gatgcattac aagccgaagt
     8701 tgacagacgt attcaacaag ctgattatac atttgcgtca ttagatatct ttaatggtct
     8761 gaaacgacgc gcacaaacga ttttagatga aaatcgtaac aatgtaccat taaataaaag
     8821 agtttctcaa gcatatattg attcattaac taatcaaatg caacatacgt taattcgaag
     8881 tgttgatgct gaaaatgcag ttaataaaaa agttgaccaa atggaagatt tagttaatca
     8941 aaatgatgaa ttgacagatg aagaaaaaca agcagcaata caagttatcg aggaacataa
     9001 aaatgaaata attggtaata ttggtgacca aacgactgat gatggcgtta ctagaatcaa
     9061 agatcaaggt atacagacct taagtgggga tactgcaaca ccggttgtta aaccaaatgc
     9121 taaaaaagca atacgtgata aagcaacgaa acaaagggaa attatcaatg caacaccaga
     9181 tgttactgaa gacgagattc aagatgcact aaatcaatta gctacggatg aaacagatgc
     9241 tattgataat gttacgaatg ctactacaaa tgctgacgtt gaaacagcta aaaataatgg
     9301 catcaatact attggagcag ttgttcctca agtaactcat aaaaaagctg caagagatgc
     9361 aattaaccaa gcaacagcaa cgaaaagaca acaaataaat agtaatagag aagcaactca
     9421 ggaagagaaa aatgcagcat tgaacgaatt aactcaagca accaaccatg ctttagaaca
     9481 aatcaatcaa gcaacaacaa atgctgatgt tgataacgcc aaaggagatg gtctaaatgc
     9541 cattaatcca attgctcctg taactgttgt taagcaagct gcaagggatg ccgtatcaca
     9601 tgatgcacaa caacatatcg cagagatcaa tgctaatcct gatgcgactc aagaagaaag
     9661 acaagcagca attgacaaag tgaatgctgc tgtaactgca gcaaacacaa acattttaaa
     9721 cgctaatacc aatgctgatg ttgaacaagt aaagacaaat gcgattcaag gaatacaagc
     9781 aattacacca gctacaaaag taaaaacaga tgcaaaaaat gccatcgata aaagtgcgga
     9841 aacgcaacat aatacgatat ttaataataa tgatgcgacg ctcgaagaac aacaagcagc
     9901 acaacaatta cttgatcaag ctgtagccac agcgaagcaa aatattaatg cagcagatac
     9961 gaatcaagaa gttgcacaag caaaagatca gggcacacaa aatatagtag tgattcaacc
    10021 ggcaacacaa gttaaaacgg atactcgcaa tgttgtaaat gataaagcgc gagaggcgat
    10081 aacaaatatc aatgctacaa ctggcgcgac tcgagaagag aaacaagaag cgataaatcg
    10141 tgtcaataca cttaaaaata gagcattaac tgatattggt gtgacgtcta ctactgcgat
    10201 ggtcaatagt attagagacg atgcagtcaa tcaaatcggc gcagttcaac cgcatgtaac
    10261 gaagaaacaa actgctacag gtgtattaaa tgatttagca actgctaaaa agcaagaaat
    10321 taatcaaaac acaaatgcaa caactgaaga aaagcaagtg gctttaaatc aagtggatca
    10381 agagttagca acggcaatta ataatataaa tcaagctgat acaaatgcgg aagtagatca
    10441 agcgcaacaa ttaggtacaa aagcaattaa tgcgattcag ccaaatattg ttaaaaaacc
    10501 tgcagcatta gcacaaatca atcagcatta taatgctaaa ttagctgaaa tcaatgctac
    10561 accagatgca acgaatgatg agaaaaatgc tgcgatcaat actttaaatc aagacagaca
    10621 acaagctatt gaaagtatta aacaagctaa cacaaatgca gaagtagacc aagctgcgac
    10681 agtagcagag aataatatcg atgctgttca agttgatgta gtaaaaaaac aagcagcgcg
    10741 agataaaatc actgctgaag tggcgaagcg tattgaagcg gttaaacaaa cacctaatgc
    10801 aactgacgaa gaaaagcagg ctgctgttaa tcaaatcaat caacttaaag atcaagcaat
    10861 taatcaaatt aatcaaaacc aaacaaatga tcaggtagac acaactacaa atcaagcggt
    10921 aaatgctata gataatgttg aagctgaagt agtaattaaa ccaaaggcaa ttgcagatat
    10981 tgaaaaagct gttaaagaaa agcaacagca aattgataat agtcttgatt caacagataa
    11041 tgagaaagaa gttgcttcac aagcattagc taaagaaaaa gaaaaagcac ttgcagctat
    11101 tgaccaagct caaacgaata gtcaggtgaa tcaagcagca acaaatggtg tatcagcgat
    11161 taaaattatt caacctgaaa caaaagttaa accagctgca cgtgaaaaaa tcaatcaaaa
    11221 agcgaatgaa ttacgtgcta agattaatca ggataaagaa gcaacagcag aagaaagaca
    11281 agtagcacta gataaaatca atgaatttgt aaatcaagcc atgacagata ttacgaataa
    11341 tagaacaaat caacaagttg atgatacaac aagtcaagcg cttgatagca ttgctttagt
    11401 gacgcctgac catattgtta gagcagctgc tagagatgca gttaagcaac aatatgaagc
    11461 taaaaagcgc gaaattgagc aagcggaaca tgcgactgat gaagaaaaac aagttgcttt
    11521 aaatcaatta gcgaataatg aaaaacgtgc attacaaaac atcgatcaag caatagcgaa
    11581 taatgatgtg aaacgtgttg aaacaaatgg cattgctaca ctaaaaggtg tacaacctca
    11641 tattgtaatt aagcctgaag cacaacaagc aataaaagca agtgcagaaa atcaagtaga
    11701 atcaataaaa gatacaccac atgcaacagt tgatgaatta gatgaagcga atcaattaat
    11761 tagcgacaca ctcaaacaag cgcaacaaga aatagaaaat acaaatcaag atgctgctgt
    11821 tactgatgtt agaaatcaaa caatcaaggc aatagagcaa ataaaaccta aagtaagacg
    11881 taaacgagct gcgcttgata gcattgaaga aaataataaa aatcaactcg atgcaatccg
    11941 aaatacgttg gatactactc aagatgaaag agatgttgct attgatactt taaataaaat
    12001 tgtaaataca attaaaaatg acattgcaca aaacaaaacg aatgcagaag tggatcgaac
    12061 tgagactgat ggcaacgaca acatcaaagt gattttacct aaagttcaag ttaaaccagc
    12121 agcgcgtcaa tctgttggtg taaaagccga agctcaaaat gcactaatcg atcaaagcga
    12181 tttatcaact gaagaagaaa gactagctgc taaacattta gtagaacaag cacttaatca
    12241 ggctattgat cagatcaatc atgcagataa gactgcccaa gttaatcaag atagtataaa
    12301 tgctcaaaat attatttcaa aaattaaacc agcgacaaca gttaaagcaa cagcattaca
    12361 acaaattcaa aatatcgcta caaataaaat taatttaatt aaagcaaata acgaagcgac
    12421 agatgaagaa caaaatattg caatagcaca agttgaaaaa gagttaatta aagctaaaca
    12481 acaaattgct agtgcagtga ctaatgcaga tgtggcatat ttattgcatg atgagaaaaa
    12541 cgaaattcgt gaaatcgaac ctgttattaa cagaaaggcg tctgctcgag aacaattgac
    12601 aacattattc aacgataaaa aacaagcaat tgaagcgaat attcaagcaa cggtagaaga
    12661 aagaaatagt atattagcac agttacaaaa tatttatgac actgctattg gacaaattga
    12721 tcaagatcgt agcaatgcac aagttgataa aacagcatca ttaaatctac aaacaataca
    12781 tgatttagat gtacatccta ttaaaaagcc agatgctgaa aaaacgatta atgatgatct
    12841 tgcacgcgtc actgctttag tgcaaaatta tcgaaaagta agtaatcgta ataaggctga
    12901 tgcattaaaa gctataactg ctttaaaatt acaaatggat gaagaattaa aaacagcacg
    12961 cactaatgct gatgttgatg cagttttaaa acgatttaat gttgcattaa gcgatataga
    13021 agcagtaatt actgaaaaag aaaatagctt actgcgaatt gataacattg ctcaacaaac
    13081 atatgcgaaa ttcaaagcga tcgcaacacc agaacaatta gctaaagtaa aagtattaat
    13141 tgatcaatat gttgcagatg gcaatagaat gattgatgaa gatgcgacat taaatgacat
    13201 caaacaacac acgcaattca ttgttgatga aattttagca attaaattac cagctgaagc
    13261 gacgaaagta tcaccaaaag aaattcagcc agctccaaaa gtttgtacgc ctattaaaaa
    13321 agaagagaca catgaatcgc gcaaagttga aaaagaactt ccaaatacag gttctgaagg
    13381 aatggattta ccattgaaag aatttgcact gattacaggt gcggctttgt tagctagaag
    13441 acgtactaaa aacgaaaaag aatcataatt aacgaaatga ccttaatata acgacacttt
    13501 ttagtgactc agttatatta aggtcatttt tatttgaatg aaaaagttga tctactaaag
    13561 agaatcgaac caaattttat actattattc cattgatgca tgtgagtaat gtccggcata
    13621 taatccagcg acatgtcctg ttacgagtgc acttgtaata ttataaccac cagtataacc
    13681 atgtatatct aatacttcac cacataaaaa taatcccgga actaatttag acatcattgt
    13741 tttaggttga atttctttaa gtgacacacc accacctgtg acaaatgcct tatctatagg
    13801 taatgtcccg ttcaccttaa atacaaaccc tttaaacata tttactaagt cgttcaattg
    13861 ttgatttgat aagtgatgtg atgtggtatt ttcatcgatt cctgcttgtt ccagcatgaa
    13921 cagtaagtag cgctcttcaa ttagaccatg caaactgttt ttaatgattt tatctggtgt
    13981 gtccgataat aatgatgtga tgtgttgttt taattgttca tggtttaatt caggaaatgc
    14041 atcgattgcc atagaaatgt gctgtgtctt ttgatttttt tgttctttat aaacaaactg
    14101 actacatctt aatgcagctg gaccactgat accaaaatga gtaaataaca tatccatttg
    14161 atgactgatg tgttttttac catttttctt aagtactgac aattcaacat cttttaaact
    14221 taaacctttt agacgattgg atttgatgaa aggttcagct gatgtaattg gaacttcggt
    14281 cgggaataac tcagtaatgg tatgacctaa atcttgtgcg aacttataac catcaccagt
    14341 tgaaccagtt tgagggacac ttgtaccacc tgtagcaatc actagcgaat ggctttcata
    14401 actattattt tgagtatgta cagtgaaagt ttggtctgta ttaacttcga ttctactaac
    14461 agcttcttct tctttaatcg ttacatgttg gcgttcgata gttgtcacta atgtatcaac
    14521 cacgtcttgt gctttgttgg aaactggaaa catacgcccg tgatcttctt cttttaattt
    14581 aacaccccta gactcaaaaa aatctatgat ggattcatta tcaaaaattg aaaagggact
    14641 atataaaaat ttcccatttc caggaatgtt cttaataatt tcagcatatg gtaatcgatt
    14701 agttacgtta catctaccgc caccagatat tttgagttta cgacctagac ctttcttttt
    14761 ttcaatgagt aacacactgc tactttgttc gcttgcagct actgccgcca ttaagccgct
    14821 aggtccgcct ccgataataa ttgtttgata catatacgct tcctccaaat ttgttactgg
    14881 acattcaatt gctattttat agtacttttt taaactttgc tatatctctt gtgttatgag
    14941 atgacaatta tgttgaatat atttataatg tgaacttcat aaaaaatgaa gatgtatgtg
    15001 tatgataaga atgatttttc tactgttatg cgatgttcat ctcaaatagt agtataatgt
    15061 ttatatgaaa tttcgtaata ttctaatcaa aatctagtat tcaatgacaa tgtgttgata
    15121 attttgttat gataattcat tgaatatacg acaatgaaat aaagttttgt gatatattca
    15181 taataatgaa aaatgatttt acagagaaac agcttttagt ttacaaaact ttaataaaca
    15241 tgagataggg agattcgtaa tgagtgaaag taaagaaatg gtgcgtggaa cctttttaat
    15301 taccattagt atattaatta ccaaggtatt gggcgtactt tttatcattc cattcaacta
    15361 tttaattggt ggacaagaaa atatggcgcc gttcacatac gcttatgcac catataatat
    15421 tgcaattgca gttgcgacag caggtgtacc attagcagct tcgaaatatg ttgcgaaata
    15481 taatgctatt ggggcatata aagtaagtca gaaattttat aaatcgagtt ttattgtaat
    15541 gagtattacc ggtgttttag gtttcttggt actgtatttc ttagcacctt atatttctga
    15601 attaacatta gctagaaaca ttcatgataa aaacggttgg tcagtcgatg atattacttg
    15661 gattataaga atcattagta tggttgttat ctttattcct gtattagcga catggagagg
    15721 gatattccaa ggttataaat ctatggggcc aacagcagtg tcagaagtaa ctgagcaaat
    15781 tgcacgcgtg attttcatat taatcggaag ttatttagta ctaaatgtct ttgatggctc
    15841 tattttatta gccaatggta ttgctacttt tgcagcagca gttggtgcga ttattggtat
    15901 attcacactc tggtattact ggagaaagcg taagcataat attgatcgta tggtcgaatc
    15961 tgattacaca gatatagatg tgtcatatgg caagatgtat aaagaaatta ttgcttatag
    16021 tataccgttc gttattgtaa gtttgaatta tccattattt aacttagtag atcaatttac
    16081 acataacggt gctttatctt tagttggtat accttcacag ttacaagata tattctttaa
    16141 tatgctaaat atgtcaacta ataaaattgt aatgattccg acatcattaa gtgctggttt
    16201 tgcagtgagt ttaattcctt atattacaaa aacatttgca gaaggtcgct tacatgaaat
    16261 gcatcatcaa attagaacat ctattggtgt attaatgttt ataactgttc cagctagtat
    16321 tggaattatg gccttggcac aaccattatt cacagttttc tatggttacg atccaattgt
    16381 tttaggtcat gaccctaacc atgatggtag tcgcttatta ttctattatg cgcctgtagc
    16441 aatactaatt tcactattaa gtgtaacggc atcaatgtta caaggtatag ataaacagaa
    16501 attaacagtt tatgttattt tagcttctgt agttatcaag ttagcgttaa actatccatt
    16561 aattatgtta ttccatacac ctggtgcaat tttaagtact agtatcgcat tattatttgc
    16621 aattggatgt aatttctata ttcttaaaaa atatgcgaag tttaaattca gctatagttg
    16681 gattcatttt gcaaaaatat tcttgtattc atttattatg atgctaggtg tagaattagt
    16741 atttttcctt gcaaatctat tcttagaacc tactaaactt ggttacttaa tcattattat
    16801 tttaggtgtt acagtaggta ttctaattta cggtacaatc acaattaaaa cgcgtcttgc
    16861 ggacgaattc ttaggtgaaa ttcctgaaaa attaagacgt agagttaggt ttttacgatg
    16921 agaatagata aatttttagc aaatatgggc gtcggtacac gtaatgaggt caaacagtta
    16981 cttaaaaaag gccttgtaaa tgtcaacgaa caagtaatta aatcaccaaa aacacatata
    17041 gaaccagaaa acgataagat aacagttcgt ggagaattaa tagaatacat tgaaaatgtc
    17101 tatattatgt taaataagcc aaaaggttat atatcagcaa ctgaagatca tcattcaaaa
    17161 accgttattg atttaatacc tgaataccaa catctaaata tttttccggt tggtcgtctt
    17221 gataaagata cagaagggct tttattgata acgaatgatg gtgattttaa tcatgaatta
    17281 atgagtccaa acaaacatgt ttctaaaaaa tatgaagtta tttcagcaaa tcctatcact
    17341 gaagacgaca ttcaagcttt taaagagggt gtaacactga ctgatggcaa ggttaagcct
    17401 gcaatattaa cttatattga taatcaaacg tcacacgtaa caatttatga aggaaaatat
    17461 catcaagtta aacgaatgtt tcatagcatt caaaatgagg tattacattt aagacgtatc
    17521 aaaattgctg atttagaatt ggacagcaat ttagattcag gagaatatcg tttattaact
    17581 gaaaatgatt ttgacaaatt aaattataaa taaaagggag acgattgata tggcaaaagg
    17641 taatttattt aaagcgattt taggtatagg tggcgctgta gcagctgtac ttgttacacg
    17701 taaagatagt cgtgacaagc tgaaagcaga atataataaa tataaacaag atcctcaaag
    17761 ctataaagat aatgctaagg ataaagcgac gcaattagga acaattgcta atgaaacaat
    17821 taaagaagta aaaacaaatc cgaaagaata tgctaataga ttaaaaaata atccaaaagc
    17881 atttttcaaa gaagaaaaat caaaatttac cgaatatgac aataagactg acgaaagtat
    17941 tgaaaaaggt aaatttgatg atgaaggtgg cgcagcacca aataataatt tacgtatcgt
    18001 cactgaagaa gatttaaaaa agaataaaaa tgcattatct gataaagaat aagatgtgag
    18061 taggacagaa atgataaggg agtgggacag aaatcatatt ttag
//
LOCUS       DALAAR010000052        17535 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000052 DALAAR010000000
VERSION     DALAAR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.054"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            631..2040
                     /gene="sapep"
                     /locus_tag="O2458_001873"
     CDS             631..2040
                     /gene="sapep"
                     /locus_tag="O2458_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCZ3887735.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            2044..2892
                     /gene="dat"
                     /locus_tag="O2458_001874"
     CDS             2044..2892
                     /gene="dat"
                     /locus_tag="O2458_001874"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCZ3887736.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            3442..4233
                     /locus_tag="O2458_001875"
     CDS             3442..4233
                     /locus_tag="O2458_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCZ3887737.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            4248..4892
                     /gene="trmB"
                     /locus_tag="O2458_001876"
     CDS             4248..4892
                     /gene="trmB"
                     /locus_tag="O2458_001876"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCZ3887738.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            5362..6204
                     /locus_tag="O2458_001877"
     CDS             5362..6204
                     /locus_tag="O2458_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ3887739.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(6329..6640)
                     /locus_tag="O2458_001878"
     CDS             complement(6329..6640)
                     /locus_tag="O2458_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ3887740.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            6727..7803
                     /locus_tag="O2458_001879"
     CDS             6727..7803
                     /locus_tag="O2458_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ3887741.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            7868..8179
                     /locus_tag="O2458_001880"
     CDS             7868..8179
                     /locus_tag="O2458_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ3887742.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            8279..9136
                     /locus_tag="O2458_001881"
     CDS             8279..9136
                     /locus_tag="O2458_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCZ3887743.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            9165..9761
                     /locus_tag="O2458_001882"
     CDS             9165..9761
                     /locus_tag="O2458_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCZ3887744.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            9782..13606
                     /locus_tag="O2458_001883"
     CDS             9782..13606
                     /locus_tag="O2458_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ3887745.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            13630..14943
                     /locus_tag="O2458_001884"
     CDS             13630..14943
                     /locus_tag="O2458_001884"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCZ3887746.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            15017..15508
                     /locus_tag="O2458_001885"
     CDS             15017..15508
                     /locus_tag="O2458_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCZ3887747.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            15582..16940
                     /locus_tag="O2458_001886"
     CDS             15582..16940
                     /locus_tag="O2458_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887748.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            17075..17179
                     /locus_tag="O2458_001887"
     CDS             17075..17179
                     /locus_tag="O2458_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887749.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT         6658 a         2375 c         3275 g         5227 t
ORIGIN      
        1 attttcattc agtcaactac tgccaatata atattgtaaa ctataggaca tttattagtg
       61 tttcaggttc tttatcgtta gtcatagcta atatgcatat agaaagaaga atacatgcgt
      121 gaaactcatt cataagaaat actaatatct aaagaaaacg tatttcttta tcttttcgaa
      181 attctctgtg ttggggccct taccccaact tgcacattat tgaaagctga ctattggcca
      241 gcttctgtgt tggggccctt accccaactt gcacattatt gaaagctaac tattggccag
      301 cttctatgtt ggggccccgc caacttgcat tgtctgtaga atttcttttc gaaattctct
      361 atgttggggc cccggggcgc attttcgttc ggttaattac tgtcaatatt aaattgtgtg
      421 cctagtacat tgacttatat ctcgagctct tgaatatgtg tattttaaat atataaatta
      481 aaaaaggaac aaagcagcta tttatgattt gtgtcatatc aatagcatcg ttttgttcct
      541 ttttattatt ttctgacatc ttattgtagt ttttatatct tttcatgtaa aatagaattt
      601 aacttataga aaaaaggaag tcgatttcga atgtggaaag aaaaagttca acaatacgaa
      661 gatcaaatca ttaatgactt aaaaggatta ttagcaattg aaagtgtgag agatgatgca
      721 aaagcatcag aagacgcacc agttggtcca ggtcctcgta aggcattaga ctacatgtat
      781 gaaattgcac atagagatgg atttacaaca catgatgtgg atcatattgc aggaagaatt
      841 gaggcaggta aaggaaatga cgtattaggt atcttatgtc atgttgacgt tgttcctgct
      901 ggtgatggat gggatagtaa tccgttcgag ccggttgtaa cagaagatgc tatcatagct
      961 agaggtacac ttgatgacaa aggtccaaca attgctgctt attatgcaat taagatatta
     1021 gaagatatga atgtggattg gaagaaacgt attcatatga ttattggtac ggatgaagaa
     1081 tctgattgga aatgtacgga tcgctatttt aaaacagaag aaatgccaac attaggtttt
     1141 gcaccagatg cagaatttcc atgtattcat ggtgaaaaag gcattacaac atttgattta
     1201 gttcaaaata aacttactga agatcaagat gaacctgatt atgaattaat aacttttaaa
     1261 tctggtgaac gttacaacat ggtacctgat catgcagaag caagagtgct tgttaaagaa
     1321 aatatgacag atgttattca agactttgag tactttttag aacaaaatca tttacaaggt
     1381 gatagtactg ttgatagtgg cattctagtt ttaacagttg aaggtaaagc ggttcatggt
     1441 atggatccat ctatcggtgt gaatgcgggt ctttacttac taaaattctt agcatcatta
     1501 aatcttgata ataatgcaca agcgtttgta gcatttagta atcgctactt atttaattca
     1561 gattttggtg aaaagatggg aatgaaattc catacagatg tcatgggtga cgtgacaact
     1621 aacattggtg ttattacata tgataatgaa aacgcaggtc ttttcggtat caacttacgc
     1681 tacccagaag gatttgaatt tgaaaaagct atggatcgtt ttgcaaatga gattcaacaa
     1741 tatggctttg aagtgaaatt aggtaaagtc caaccaccac attatgttga taaaaatgat
     1801 ccttttgtac aaaagttagt tactgcatat agaaatcaaa caaatgatat gactgaacct
     1861 tatactatag gtggcggtac ttatgcgaga aacttagaca agggtgtagc atttggcgca
     1921 atgtttagtg attctgaaga tttaatgcat cagaaaaatg aatatatcac taaaaaacag
     1981 ttatttaacg caactagtat ttacttagaa gcaatttatt cattatgcgt ggaggaataa
     2041 tatatggaaa aaattttttt aaatggtgag tttgtaagtc caagtgaagc aaaggtttca
     2101 tacaacgaca gaggatacgt atttggcgat ggtatttatg aatacattcg agtatataat
     2161 ggtaagttat ttacagtaac agaacattat gaaagatttt tacgtagtgc caatgagatt
     2221 ggtttagatt taaattattc tgtagaagaa ttaattgaac tatctcgtaa attagttgat
     2281 atgaatcaaa ttgaaactgg ggcaatttat attcaagcaa cgcgtggtgt agctgaaagg
     2341 aatcatagct tcccgacacc tgaagtagaa ccagcaattg ttgcttatac aaagagttat
     2401 gatcgtcctt atgatcattt agaaaatggt gtgaatggtg ttaccgttga agatatccga
     2461 tggttacgtt gcgacattaa aagcttgaac ttattaggaa atgtattagc aaaagaatat
     2521 gctgtgaaat ataatgcagt tgaagcaatt caacatcgag gtgaaactgt aactgaagga
     2581 tcttcaagta atgcttatgc aattaaagac ggtgtgattt atacacatcc gattaacaac
     2641 tatattctta atggtattac acgaattgta attaaaaaaa ttgccgaaga ctataacatc
     2701 ccatttaaag aagaaacgtt tactgtagat ttcttgaaaa acgcagatga agttattgtt
     2761 tcaagtactt cagctgaggt tacacctgtt attaaattag atggtgaacc agttaatgat
     2821 ggtaaagttg gcccaattac acgtcaacta caagaaggat ttgaaaagta tatagagtca
     2881 cacagtattt aaaattcttt catcatattt ttagattaaa ttacttaaat ttatcaactt
     2941 tcgtaagtga attatgttat aatttaaaac gaagcttaaa agtgttgttt agaaaacaca
     3001 tattagtaaa aagtgccatt ttgtttctca ttttcagttg aaatataatg ataaaaatta
     3061 taaaattttc tatgagttac aaatatgatg tgcatcatat tttactaagt acgtaaatgt
     3121 tacagttcat aaatttatgc taaccatgaa aatgggatag caattatttt gaaaactaga
     3181 gtttatcgcc tacagagatt caaaaacatg tagttttaga cacggtaaca ttatatgtca
     3241 agtaagtgac tatagaatgt atggtgtcaa tgacagacat tacgatttaa atttagtcgt
     3301 gtaatgtagg cattggttga ggatttacaa taattatctg gacataatat ttgtaccagt
     3361 atggtgttat aaattagaat atatggctct tgaaaaacct atttctagag ccattttcta
     3421 attgaaagtg aggtggacga gttggagcag ttttatcaat tagggtggac acttgattca
     3481 gcaggtggtg catctggtga agcatatatg gctgaacaag atggacaaaa gttgttttta
     3541 aaacgaaatt caaatccatt tattgcggca ttatcagcag aaggtattgt gcccaaatta
     3601 gtatggacga aacgcataga aacaggcgag gttgttacag cacaacattg gaaaaatggg
     3661 cgtgaactat cttcaaacga aatgaagcaa acaagagttg cacatttatt aaagaagata
     3721 cacaattcta gacctttatt aagtatgtta aagcgtatgg aaatggaacc tattactcct
     3781 gagattatgc ttaataaaat taatgcctct ttatcaagag aagttttaac acatcatatt
     3841 gtgagaaaat cattaaccta tttagaagag catataccga gtttagattc gcgttttttc
     3901 actgttgtac atggtgatgt gaatcataat aattggttat tatccgatcg agatgaacta
     3961 tttttagttg attgggaagg tgcaatgatt gcagacccgg caattgatat cggaatgctg
     4021 ctttacaact atgttccaca acaacaatgg tctgaatggc ttgaaacata tggtgttcaa
     4081 gagagcttga atttaaataa acgtatgaaa tggtatacgg taattcaatc tataggtctt
     4141 gttcaatggt atgaagagca aaaaagatat aaggatatga acacttggct taaattccta
     4201 aatgaagtta tgaatagtaa catgtttatt taagaggaga ataaaatatg agagttcgat
     4261 acaaaccatg ggctgaagat tatttaaaag atcatcctga attggttgac atggacggtc
     4321 aacatgcagg gaaaatgact gaatggtttg ataaaacgca accgatacac attgaaattg
     4381 gttcgggtat ggggcagttt attacaacat tagctgctca aaatcctcat attaactata
     4441 tttcaatgga acgtgagaaa agtatcgttt ataaagtatt ggataaagtt aaagagatgg
     4501 gtttaacaaa tttaaagatt atttgtaatg acgccattga attaaatgaa tattttaaag
     4561 acggcgaagt ttcgcgtatt tatttgaact tttcagatcc atggccaaaa aatcgtcatg
     4621 caaaacgacg tttaacgtat catacgtttt tagcgttgta tcaacaaatc ttaaatgatg
     4681 aaggagattt acattttaaa actgataaca gaggactatt tgcctacagt ttagaaagta
     4741 tgtctcaatt tggaatgtat tttacaaaaa taaatctaaa tttacatcaa gaagacgatg
     4801 gcagtaatat tttaacagaa tatgaaaaga aattttcgga taaagggtca cgtatttatc
     4861 gtatggaagc gaaattccat tcacaaaaat agagcactta tgagggtatg tacacataca
     4921 atcataagtg ttttttcgta ctataatggt tctatagata aaggttctat aataaatcgg
     4981 actgatggaa aaagttttta acttttcatc tgtccgattt tttgattttg aatataaaaa
     5041 aagcgcaatt atctctataa ttaaaagtga ctaaacaaat aactaaagga gataatgcgc
     5101 ctatgaacaa tgttatacta aatacctttg atttaaaaga ataaagcttg cattttactt
     5161 gtaaatttta attattaatt taaaaataca ggccgatggt tatgatgcat ttacatataa
     5221 tatgattgca ttaatcttta taagtagtta gaagtgtatt agtgtggggc gtttcgtttt
     5281 tgttcgcatt tatgaatagt gatatgatgt aataaacaca tgctaattgt aataacgaat
     5341 acagaaagaa taggtgatgt tatgaaaatc ggggatatat ctattcatta tctaaatggt
     5401 ggcaatacaa aaatggatgg cggtgcaatg tttggtgttg ttccgaagcc gttgtggtca
     5461 aagcaataca atgcaaatga acgaaatcaa atcaatttac cgacacatcc aattttgatt
     5521 caaacggctc aatataattt gattatagat gcaggtattg gtaatggtaa attatctgaa
     5581 aagcaattac gtaattttgg agtagatgaa gaaagtcata taattgctga tttggcaaat
     5641 tataatttaa cgccaaagga tattgattat gtgctaatga cacatatgca ttttgatcat
     5701 gctgccggtt tgactgatca agcaggacat gcaatttttg aaaatgcgat tcatgttgtg
     5761 caacaagatg agtggcatga gtttattgca cctaatataa gaagtaaatc aacttactgg
     5821 gataagaata aaggcgatta tagtaacaag ttgattttat tcgaaaaaca ttttgaaccg
     5881 gttctgggta tcaagatgca acatagtgga ggtcatagct ttggccacac gattattacg
     5941 attgaaagtc aaggagataa agcagttcat atgggtgata tattcccaac tactgcacat
     6001 aaaaatcctc tatgggtaac ggcatatgat gattatccta tgcaatcgat tcgtgaaaaa
     6061 gaacgcatga taccatattt tattcagcaa caatattggt tcttgtttta tcatgatgaa
     6121 aactactttg ctgtaaaata cagcgataat ggtgaaaaca tagatgcata tattttacgt
     6181 gaaacattag ttgataataa ctaaaataaa gatgtattac taaacaaatt ttcaaaaata
     6241 aaaaattgag ccacatccaa tcttactaat tagggtgtgg ctcattttta agttttacga
     6301 tccaaatcaa atatggataa aattcgtatt aacgctctac aatgttaatg acttcaccag
     6361 tatatgcatc tgcataaaaa tcataatgaa tattttgacc atttttaata gttgtaattc
     6421 caccttgata aactaaacgg tatttatcag tttcaggatg aacgaatggt tcataaacga
     6481 tatatgagcc tttgacattc ataaaatatg attttacttc attaagtact ttaacgggat
     6541 tatagcgttt acgattaata aattggatga ttgaaatggt agaaataatt acaatggcta
     6601 ctgcaattat tgttggaatt atatatttca gtttagtcat catttatgct cccttgattt
     6661 caaattcata tcattagttt accatattga agatgatata ataacttata aggagtgaga
     6721 acttttatga acataaataa aaaagtaaca ttacaacgaa ttcaaacttt aactgagctt
     6781 catggggcac caggttttga agaagaagta aaaaattata tgactcagca aatggcgccg
     6841 tacgtagatg aatttattga aaatcgtatg ggtggatttt ttggtgtgaa aaaatctaaa
     6901 aatccaaatg caaaacgtgt aatgattgca gcacatatgg atgaaatcgg atttatgatt
     6961 acaaatatca ctaaaaatgg aatgattcaa ttcacaaatt taggtggtgt tgcaaatgat
     7021 atttggcaag gacaacgctt agtaattaaa aatagaaatg gcgataaaat tatcggtgtt
     7081 gtttctaata tacctaaaca ttttcgtact ggtagtgaag gtgcaccgga aattaaagat
     7141 ttaacattag atataggtgc tcaaaatgaa gatgaggtgc gtgagcgcgg aatagatata
     7201 ggagatacaa ttgtacctca cacgccattc acacagttat ctgaacatcg atatagtgct
     7261 aaagcatggg ataatcgtta tggttgtgtc ttggcaattg aaatactaga attattaaaa
     7321 gatatagaat tagatgtaga cttgtatgtt ggcgcaaatg ttcaagaaga ggttggatta
     7381 cgaggtgcga aagcatctgc agagatgata gacccagacg ttgcatttgt agttgattgt
     7441 tcacctgcca atgacgttaa aggaaaccaa ccattatctg gtgaacttgg taaagggacg
     7501 ttaattcgca taaaagacgg tacaatgatt ttaaagcctg tatttagaga ctatttatta
     7561 aagttagtag aagcacatga cattgaacat caatactata tgtcaccagg tggaacagat
     7621 ggtggagaaa ttcataaagc taatattggt attccgactg cagttattgg tgtatgtgca
     7681 cgatatattc atagtacaga ctcagtattt gatataagag actattttgc agctagatct
     7741 ttactttcag aagccatttg taatttagat aataatcaaa tagaaacatt acaatataaa
     7801 taatcgggta ataacaacta ttatctctaa atagttatat ataatcatta attaaggaga
     7861 cataaaaatg aaacaacttg aatcagaaca acaatttgaa tctttaaaac aaggtgctac
     7921 agtatttgaa ttcactgcag gctggtgtcc agattgtaga gtgatagaac cagatttacc
     7981 ggaattagaa gcgagatatc ctatgtttga cttcgtatca gtagaccgtg ataaatttat
     8041 ggatatttgt attgaaaatg gtattatggg tattccaagt tttctagtat ataaaaatgg
     8101 agaactgctt ggaagttata ttggaaaaga acgaaaatca attgaacaga tagatgcatt
     8161 tttagctcaa tacgtgtaat ttagactaga gaaaaacggg gtaatactcg tttttctctg
     8221 ttactatgtg ttgatttatt gtaaactatt ataaggtgcg aaattaggag tgttacatat
     8281 gaataccttt caaatgagag ataaattaaa ggaacgttta agccatttag acgttgattt
     8341 taaatttaat cgtgaagaag aaactttgcg tatttatcga acagataata acaaaggtat
     8401 cacgattaaa cttaacgcta tagtcgcaaa atatgaagat aaaaaagaaa aaattgtaga
     8461 tgaaattgtt tattacgttg atgaagctat tgcacaaatg gcagataaaa cccttgagag
     8521 tatatcatct agtcaaatta tgcctgtcat tagagcgact agctttgata aaaaaactaa
     8581 acaaggtgtt ccttttatct atgatgagca tactgcagaa acagcagttt attatgcagt
     8641 cgatttaggg aaatcatatc gtctaattga cgaaagcatg ttagaggatt tgaagttaac
     8701 tgaacaacaa ataagagaaa tgtctctgtt taatgttaga aaattgtcaa attcatatac
     8761 gactgatgaa gtaaaaggta atatttttta ttttattaac tcaaatgacg ggtatgatgc
     8821 aagtaggata ctaaatactg catttttaaa tgaaattgag gcacaatgtc aaggcgaaat
     8881 gctcgtagca gtgccacacc aagatgtgtt aattattgca gatatacgca ataaaacagg
     8941 atatgatgtg atggcacatt taacaatgga atttttcact aaaggtctag ttccaattac
     9001 atcattatcc tttggatata aacagggtca tcttgaaccg atatttattt taggtaaaaa
     9061 taataaacaa aaaagagatc caaacgtgat tcagcgttta gaagcaaatc gtcgtaaatt
     9121 taataaagat aaatagaaat aattggataa ggagttttgt cataatgaat ttattttaca
     9181 atcctaaata tgtaggagat gtcgcatttt tacaaattga accagttgaa ggtgaattaa
     9241 actacaataa aaaaggtaat gttgttgaaa ttactaatga aggtaatgtt gtaggttata
     9301 atatttttga aatttcaaaa gatataacaa ttgaagaaaa aggtcatatt aaattaactg
     9361 atgaacttgt aaatgtattc caaaagcgta tttcagaagc tggttttgat tataaattaa
     9421 atgctgatct atcaccgaaa tttgtagttg gctacgttga aactaaagac aaacatcctg
     9481 atgcagataa attaagtgta ctaaatgtaa acgttggaaa tgacacatta caaattgtat
     9541 gtggcgcgcc taacgttgaa gctggacaga aagttgttgt tgctaaagta ggtgcagtga
     9601 tgcctagcgg tatggtaatt aaagatgctg aattacgtgg tgttgcctca agcggtatga
     9661 tttgttcaat gaaagaattg aatttaccta atgcacctga agaaaaaggt attatggtat
     9721 taaatgacag ctatgaaatt ggacaagcat tttttgaata attaaggaag gtagtgaaaa
     9781 tatgagctgg tttgataaat tattcggcga agataatgat tcaaatgatg acttgattca
     9841 tagaaagaaa aaaagacgtc aagaatcaca aaatatagat aacgatcatg actcattact
     9901 gcctcaaaat aatgatattt atagtcgtcc gaggggaaaa ttccgttttc ctatgagcgt
     9961 agcttatgaa aatgaaaatg ttgaacaatc tgcagatact atttcagatg aaaaagaaca
    10021 ataccatcga gactatcgca aacaaagcca cgattctcgt tcacaaaaac gacatcgccg
    10081 tagaagaaat caaacaactg aagaacaaaa ttatagtgaa aaacgtggga attctaaaat
    10141 atcacagcaa agtataaaat ataaagatca ttcacattac catacgaata agccaggtac
    10201 atatgtttct gcaattaatg gtattgagaa ggaaacgcac aagccaaaaa cacataatat
    10261 gtattctaat aatacaaatc atcgtgctaa agattcaact ccagattatc acaaagaaag
    10321 tttcaagact tcagaggtac cgtcagctat ttttggcaca atgaaaccta aaaagttaga
    10381 aaatggtcgt atccctgtaa gtaaaccttc agaaaaagtt gagtcagata aacaaaaata
    10441 tgataaatat gtagctaaga cgcaaacgtc tcaaaataaa caattagaac aagaaaaaca
    10501 aaatgatagt gttgtcaaac aaggaactgc atctaaatca tctgatgaaa atgtatcatc
    10561 aacaacaaaa tcaatgccta attattcaaa agttgataat actatcaaaa ttgaaaatat
    10621 ttatgcttca caaattgttg aagaaattag acgtgaacga gaacgtaaag tgcttcaaaa
    10681 gcgtcgattt aaaaaagcgt tgcaacaaaa gcgtgaagaa cataaaaacg aagagcaaga
    10741 tgcaatacaa cgtgcaattg atgaaatgta tgctaaacaa gcggaacgct atgttggtga
    10801 tagttcatta aatgatgata gtgacttaac agataatagt acagatgcta gtcagcttca
    10861 tacaaatggc atagagaatg aaactgtatc aaatgatgaa aataaacaag cgtcaataca
    10921 aaatgaagac actaatgaca ctcatgtaga tgaaagtcca tacaattatg aggaagttag
    10981 tttgaatcaa gtatcgacaa caaaacaatt gtcagatgat gaagttacgg tttcgaatgt
    11041 aacgtctcaa catcaatcag cactacaaca taacgttgaa gtaaatgata aagatgaact
    11101 aaaaaatcaa tccagattaa ttgctgattc agaagaagat ggagcaacga ataaagaaga
    11161 atattcagga agtcaaatcg atgatgcaga attttatgaa ttaaatgata cagaagtaga
    11221 tgaggatact acttcaaata tcgaagataa taccaataga aacgcgtctg aaatgcatgt
    11281 agacgctcct aaaacgcaag agtacgcagt aactgaatct caagtaaata atatcgataa
    11341 aacggttgat aatgaaattg aattagcacc gcgtcataaa aaagatgacc aaacaaactt
    11401 aagtgtcaac tcattgaaaa cgaatgatgt gaatgataat catgttgtgg aagattcaag
    11461 catgaatgaa atagaaaaga ataacgcaga aattacagaa aatgtgcaaa acgaagcagc
    11521 tgaaagtgaa caaaatgtcg aagagaaaac tattgaaaac gtaaatccaa agaaacagac
    11581 tgaaaaggtt tcaactttaa gtaaaagacc atttaatgtt gtcatgacgc catctgataa
    11641 aaagcgtatg atggatcgta aaaagcattc aaaagtcaat gtgcctgaat taaagcctgt
    11701 acaaagtaag caagctgtga gtgaaagaat gcctgcgagt caagccacac catcatcaag
    11761 atctgattca caagagtcaa atacaaatgc atataaaaca aataatatga catcaaacaa
    11821 tgttgagaac aatcaactta ttggtcatgc agaaacagaa aatgattatc aaaatgcaca
    11881 acaatattca gagcagaaac cttctgttga ttcaactcaa acggaaatat ttgaagaaag
    11941 tcaagatgat aatcaattgg aaaatgagca agttgatcaa tcaacttcgt cttcagtttc
    12001 agaagtaagc gacataactg aagaaagcga agaaacaaca catccaaaca atactagtgg
    12061 acaacaagat aatgatgatc aacaaaaaga tttacagtca tcattttcaa ataaaaatga
    12121 agatacagct aatgaaaata gacctcggac gaaccaacaa gatgttgcaa caaatcaagc
    12181 tgtacaaaca tctaagccga tgattcgtaa aggcccaaat attaaattgc caagtgtttc
    12241 attactagaa gaaccacaag ttattgagtc ggacgaggac tggattacag ataaaaagaa
    12301 agaactgaat gacgcattat tttactttaa tgtacctgca gaagtacaag atgtaactga
    12361 aggtccaagt gttacaagat ttgaattatc agttgaaaaa ggtgttaaag tttcaagaat
    12421 tacggcatta caagatgaca ttaaaatggc attggcagcg aaagatattc gtatagaagc
    12481 gcctattcca ggaactagtc gtgttggtat tgaagttccg aaccaaaatc caacgacagt
    12541 caacttacgt tctattattg aatctccaag ttttaaaaat gctgaatcta aattaacagt
    12601 tgcgatgggg tatagaatta ataatgaacc attacttatg gatattgcta aaacgccaca
    12661 cgcactaatt gcaggtgcaa ctggatcagg gaaatcagtt tgtatcaata gtattttgat
    12721 gtctttacta tataaaaatc atcctgagga attaagatta ttacttatcg atccaaaaat
    12781 ggttgaatta gctccttata atggtttgcc acatttagtt gcaccggtaa ttacagatgt
    12841 caaagcagct acacagagtt taaaatgggc cgtagaagaa atggaacgac gttataagtt
    12901 atttgcacat taccatgtac gtaatataac agcatttaac aaaaaagcac catatgatga
    12961 aagaatgcca aaaattgtca ttgtaattga tgagttggct gatttaatga tgatggctcc
    13021 gcaagaagtt gaacagtcta ttgctagaat tgctcaaaaa gcgagagcat gtggtattca
    13081 tatgttagta gctacgcaaa gaccatctgt caatgtaatt acaggtttaa ttaaagccaa
    13141 cataccaaca agaattgcat ttatggtatc atcaagtgta gattcgagaa cgatattaga
    13201 cagtggtgga gcagaacgct tgttaggata tggcgatatg ttatatcttg gtagcggtat
    13261 gaataaaccg attagagttc aaggtacatt tgtttctgat gacgaaattg atgatgttgt
    13321 tgattttatc aaacaacaaa gagaaccgga ctatctattt gaagaaaaag aattgttgaa
    13381 aaaaacacaa acacaatcac aagatgaatt atttgatgat gtttgtgcat ttatggttaa
    13441 tgaaggacat atttcaacat cattaatcca aagacatttc caaattggct ataatagagc
    13501 agcaagaatt atcgatcaat tagagcaact cggttatgtt tcgagtgcta atggttcaaa
    13561 accaagggat gtttatgtta cggaagcaga tttaaataaa gaataattat gagtaaggag
    13621 ttttatataa tgacacacta tcattttgtc ggaattaaag gttctggcat gagttcatta
    13681 gcacaaatca tgcatgattt aggacatgaa gttcaaggat cggatattga gaactacgta
    13741 tttacagaag ttgctcttag aaataagggg ataaaaatat taccatttga tgctaataac
    13801 ataaaagaag atatggtagt tatacaaggt aatgcattcg cgagtagcca tgaagaaata
    13861 gtacgtgcac atcaattgaa attagatgtt gtaagttata atgatttttt aggacagatt
    13921 attgatcaat atacttcagt agctgtaact ggtgcacatg gtaaaacttc tacaacaggt
    13981 ttattatcac atgttatgaa tggtgataaa aagacttcat ttttaattgg tgatggcaca
    14041 ggtatgggat tgcctgaaag tgattatttc gcttttgagg catgtgaata tagacgtcac
    14101 tttttaagtt ataaacctga ttacgcaatt atgacaaata ttgatttcga tcatcctgat
    14161 tattttaaag atattaatga tgtttttgat gcattccaag aaatggcaca taatgttaaa
    14221 aaaggtatta ttgcttgggg tgatgatgaa catctacgta aaattgaagc agatgttcca
    14281 atttattatt atggatttaa agattcggat gacatttatg ctcaaaatat tcaaattacg
    14341 gataaaggta ctgcttttga tgtgtatgtg gatggtgagt tttatgatca cttcctgtct
    14401 ccacaatatg gtgaccatac agttttaaat gcattagctg taattgcgat tagttattta
    14461 gagaagctag atgttacaaa tattaaagaa gcattagaaa cgtttggtgg tgttaaacgt
    14521 cgtttcaatg aaactacaat tgcaaatcaa gttattgtag atgattatgc acaccatcca
    14581 agagaaatta gtgctacaat tgaaacagca cgaaagaaat atccacataa agaagttgtt
    14641 gcagtatttc aaccacacac tttctctaga acacaggcat ttttaaatga atttgcagaa
    14701 agtttaagta aagcagatcg tgtattctta tgtgaaattt ttggatcaat tagagaaaat
    14761 actggcgcat taacgataca agatttaatt gataaaattg aaggtgcatc gttaattaat
    14821 gaagattcta ttaatgtatt agaacaattt gataatgctg ttattttatt tatgggtgca
    14881 ggtgatattc aaaaattaca aaatgcatat ttagataaat taggcatgaa aaatgcgttt
    14941 taatatgttt ataatagagt agtatgggta tttattatta atgacattat tacatgttaa
    15001 ttaggaggcg tttttaatgg attggatttt accaattgct ggaattatcg ctgcgattgc
    15061 attcttaatt ttatgtatcg gtatcgtagc tgtattaaat tctgttaaga aaaacttaga
    15121 ttatgttgca aaaacacttg acggtgtaga aggtcaagtt caaggtatta ctcgtgaaac
    15181 aacagattta cttcataaag taaaccgttt aactgaggat atccaaggta aagtagatcg
    15241 tttaaactca gttgtagatg ctgttaaagg tatcggtgac tcagtacaaa cgttaaacag
    15301 ctctgtagat cgtgtaacaa attcaattac acataatatt tctcaaaatg aagataaaat
    15361 ctcacaagtt gttcaatggt caaatgttgc aatggaaatt gcagacaaat ggcaaaatag
    15421 acactaccgt cgtggaagtg caaattacaa agctaataat gtagcaactg atgcaaatca
    15481 tagctatact tctagagtag ataaataatt aataattaaa caaatgcact taaatgaggt
    15541 tgttacttat ttaagtgcat ttgttatagg aggatataca aatgacgcag caacaaaatg
    15601 ataaaagaac attaaaaaat aaacacactt atcaaaatga accactacca aaccgtaaag
    15661 attttgttgt tagttttata actggcgcgc ttgttggttc agctttaggt ttgtatttta
    15721 aaaataaagt ttatcaaaaa gcagatgatt taaaagtcaa agaacaagaa ctgtcgcaaa
    15781 agtttgaaga aagaaaaacg caacttgaag aaacggttgc ctatacaaaa gaacgtgttg
    15841 aaggattttt aaacaaatct aaaaatgagc aagcggcatt aaaggcacaa caagcagcga
    15901 taaaagaaga agcaagtgca aataatttaa gtgatacatc acaagaagca caagagattc
    15961 aagaagctaa aagagaagca caagcagaag cggataaaag tgtggctgta tcaaataaag
    16021 aatcaaaagc agtggcattg aaagcacaac aagcagcgat aaaagaagaa gcaagtgcaa
    16081 ataatttgag tgatacatca caagaggcac aagagattca agaagctaaa aaagaagcac
    16141 aagcagaaac agataaaagt gcagctgtat caaatgaaga accaaaagca gtggcattga
    16201 aagcacaaca agcagcgata aaagaagaag caagtgcaaa taatttaagt gatatatcac
    16261 aagaggcaca agaggttcaa gaagctaaaa aagaagcaca agcagagaaa gacagtgaca
    16321 cattaactaa agatgcaagt gcagcaaagg tagaagtatc aaaaccagag tcacaagctg
    16381 aaagattagc aaacgctgca aaacagaagc aagctaaatt aacaccaggt tcaaaagaga
    16441 gtcaattaac tgaagcgtta tttgcagaaa aaccagttgc taaaaatgac ttgaaagaaa
    16501 ttcctcaatt agttactaaa aagaatgatg tatcagagac agagacggtt aatatagata
    16561 ataaagacac tgttaaacaa aaagaagcta aatttgaaaa tggtgttatt acacgtaaag
    16621 ctgatgaaaa aacaactaat aatacagctg ttgacaagaa atcaggtaaa caatctaaaa
    16681 aaacaacacc ttcaaataaa cgaaatgcat caaaagcatc tacaaataaa acttcaggtc
    16741 agaaaaagca acataataag aaatcatcac aaggtgcaaa gaaacaaagt agttcaagta
    16801 agtcaactca aaagaataat caaactagta ataagaattc aaaaacaaca aatgctaaat
    16861 catccaatgc atcaaaaacg ccaaatgcta aagttgagaa agctaaaagt aaaatagaga
    16921 aacgtacatt caatgactaa gattactaaa atcgttgaat aatcaatcat agcagtaagc
    16981 tatgtaatat atttttaaaa attgaatttc aaaataaaag gtaggcattc aggtgcttac
    17041 cttttttgat atcatcagtt tattttgaaa ggcaatgcga tcatttcatg tatttatgtt
    17101 gtttgaaaca tcgttagata acaatagtga tattgcacat tttaagctga agatggaaaa
    17161 atattcgata atcaaataaa caatgaattt tagaaggtac aatgacgttc actaatttaa
    17221 atatagctga atgtgttggt gagtgatgtt cactatagat ttatattaat atacaaagac
    17281 aaaggttgtt aatttttatt aagcgttagg ttgaatgtat gagaatttta gatttataat
    17341 agaagataga aacgaaaatt tttcttaaaa gcagtaatgt tgactcaaaa taagctataa
    17401 taatgacact tatttaattg attaacattt gctaataaat atcaatatag aatataactt
    17461 tccaataatg actgagaaaa tcgaaatgtc agtctcgaat catataatta gaaaattgat
    17521 tattttctgt caatt
//
LOCUS       DALAAR010000053        17004 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000053 DALAAR010000000
VERSION     DALAAR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.055"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..677
                     /locus_tag="O2458_001888"
     CDS             <1..677
                     /locus_tag="O2458_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3887750.1"
                     /translation="IKKSFEKTLSMYPIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSE
                     MVVQPKGERMKSKGMVLYMNRNTKTTTGKYIVSETLHDEDGRPKSKDKEYPVKMVDNK
                     IIPTKGIKDENIKKEIENFKFFAQYGSFKDLSKYKDGDISYNPEVPSYSAKYQLTNDD
                     YNVKQLRKRYKIPTNKAPKLLLKGSGDLKGSSVGYKDIEFTFVEKKGENTFFTDSLHL
                     EPSEDK"
     gene            771..3008
                     /locus_tag="O2458_001889"
     CDS             771..3008
                     /locus_tag="O2458_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3887751.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            3159..4337
                     /locus_tag="O2458_001890"
     CDS             3159..4337
                     /locus_tag="O2458_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ3887752.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            4339..5727
                     /locus_tag="O2458_001891"
     CDS             4339..5727
                     /locus_tag="O2458_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887753.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(6220..7881)
                     /locus_tag="O2458_001892"
     CDS             complement(6220..7881)
                     /locus_tag="O2458_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCZ3887754.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(8207..9982)
                     /locus_tag="O2458_001893"
     CDS             complement(8207..9982)
                     /locus_tag="O2458_001893"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCZ3887755.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(10156..11028)
                     /locus_tag="O2458_001894"
     CDS             complement(10156..11028)
                     /locus_tag="O2458_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ3887756.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            11141..12538
                     /locus_tag="O2458_001895"
     CDS             11141..12538
                     /locus_tag="O2458_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ3887757.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            12728..13201
                     /locus_tag="O2458_001896"
     CDS             12728..13201
                     /locus_tag="O2458_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCZ3887758.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            13463..15055
                     /locus_tag="O2458_001897"
     CDS             13463..15055
                     /locus_tag="O2458_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ3887759.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(15384..16910)
                     /gene="spa"
                     /locus_tag="O2458_001898"
     CDS             complement(15384..16910)
                     /gene="spa"
                     /locus_tag="O2458_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCZ3887760.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
BASE COUNT         5575 a         2616 c         3076 g         5737 t
ORIGIN      
        1 aaataaagaa aagttttgag aagacattga gtatgtatcc gattaaaaat ctagaggatt
       61 tatacgataa agaaggttat cgtgatgacg aatttgataa aaatgataaa ggtacatgga
      121 ttattggttc tgaaatggtt gttcaaccta aaggggaacg tatgaaatca aaaggtatgg
      181 ttctatatat gaatagaaac actaagacta caaccgggaa gtatatagtg agtgaaacac
      241 ttcatgatga agatggtaga cctaagagta aagataaaga atatcccgtt aaaatggtag
      301 acaataaaat cattccaact aaaggtatca aagacgaaaa tataaaaaaa gaaatcgaga
      361 attttaagtt cttcgcgcaa tatggcagct ttaaagattt gtcgaagtac aaagacggcg
      421 atatatcata taatccagag gtaccgagtt attcagcaaa atatcaatta actaatgatg
      481 actataatgt aaaacaatta cgaaaaagat ataaaatacc aacgaataaa gcaccaaagt
      541 tattgttgaa aggttcggga gacttaaaag gttcatcagt tggatataaa gacattgaat
      601 ttacttttgt ggagaaaaaa ggtgaaaata ctttttttac tgatagtcta catcttgaac
      661 cgagtgagga taaataatca tccacacaca cgattcaata tgaaattcaa atatgttgct
      721 gtaaaataat gtaaaataaa cgtattcata ttactgaatt gagggatagt atgcaacgtg
      781 attatttaat tcgagtagaa actgagagta tgtcagattt caaaaggctc aatagtttaa
      841 tgattggttt tgttattaaa ggtgaggcac acatttatga tgaaaataat atgacgcaat
      901 gcaacagtgg cgacattttc atcattaacc accgcgactt gtatcgattt caacttcaac
      961 aagatggcat catatgttat atccaattcc aaatgaaata tttagcagac aagtttgatg
     1021 atgtgcattg tctatatttt cacttaacag atgcgaccac aacaaagaat atacatcaac
     1081 tgagaaatat aatggcaaga ctggtttcaa cacatatccg acataatgag ttgtctaaat
     1141 tgactgagca acaacttgtg attcagttgc ttatgcatat gattcattat gtcccgcgta
     1201 catatcattc gaaccaaagt atcttaaatg atgataaagt gaatcaagta tgcgactata
     1261 tcgagttaca ttttcatgaa gatttaagcc tttcagaatt aagcgaatac gttgggtggt
     1321 cagagagcca tctgtctaaa aagtttacag aatcgctagg tgtaggattc catcatttct
     1381 taaatacgac gcgaattgag catgcgaaac tcgatttgac atacacagat gaaaccatta
     1441 ctgatattgc attgcaaaat ggcttttcaa gtgcagcgag ctttgcgaga acatttaaac
     1501 actttacgca tcaaacacct aaacaatatc gaggtgatcg tccagcaatc actgaaaacc
     1561 agcaatcggc acaacatgat tatcacgacc gtgaattgat attactttta aatgactaca
     1621 ttgaagaaat gaatcatttt attgaagata ttgaaaagat gaactataaa gagattgcct
     1681 ttcaaccaac caatcaccaa ctaaatcaat ttaatcatat tattcaagtg ggctatttga
     1741 ggaatttgct caatacacag tatcaatcac agttgcttac atgtcatcat gattttcaag
     1801 tcaatgaagt attagcatat gatgtgattc catatatcat gaaaaagctc aatgcgccat
     1861 tcacgtatga tgcagaaatt tcgaatatat tttatgatat tgatttgtgt ttagactttt
     1921 tattagatca taactttagt ttgaccatgc atttggatca gtatgactca cgtgattata
     1981 tcgatgcatt taaaattttt atccatcacg ttgccctgca tgtcagtcat agaaaagatt
     2041 tgaagttcaa cttgtatgtg acgacattgc acacgtcttt gattgaaatg attgattatt
     2101 ttaaagcatt attccctaat ggtggcttgt acattcactt agatcaagct acggaaagac
     2161 atctaccatt attgaaacga cttgagccac acatcgacca ttttgtattt gatgccaatt
     2221 caaatgacgc tgttgatttt aataaaatga atgatgatga atttaaaacc gcgagtcaaa
     2281 tgattattaa taaaacgaat taccttatcg acttaatgca tcgtcatcgc ctaaagcgtc
     2341 cactcatttt actcaattgg aatacattga cgggtgatac atttattaca aacggcgaat
     2401 attttagagg tggtatcatc attgagcaat tattaaaatt aagttctaaa gtagagggta
     2461 tcggttattg gttgaattat gatttgcacg tcagtcattg taaaaatgaa cgggattata
     2521 tgaattctat tgaactattt catcaatata atggaaaacg tccggtctat ttcacggcat
     2581 tgctatttaa taaattaaca agcaatattt tgtattctga tgatacatgt attgtcacgg
     2641 ggactgattc aaattttcaa atattgttat atgatgcaaa gcattttaat ccgtacttag
     2701 cgttggacaa tcaaatgaat atgcgtgcga cggaaatgat tcatttgaac attaatgccc
     2761 tggaagaagg tatgtataag attaaacatt ttaccttaga taaagaaaat ggtgcgttat
     2821 ttaatctttg gcgcaaacat catacgatac atggcatgga caaggactct atagattacg
     2881 ttaatcgaat gagttttccg aaattagaag tatatgatat agatatcacg gacacactgg
     2941 cattaaacat taaaatgatt acgaatggga ttcacttaat tgaagtaaaa cgttacccaa
     3001 gttcataaaa tgatcacaaa tcacaaattt tgatatacat aatttgtgat tttttatatt
     3061 caaagctaaa attgcaaaaa attaatggtt aacatctctg ttgttggcaa tataaataat
     3121 gattaatcat ttatgatgta actaaggaga tgaaggatat gaatcaacaa ttaattgaaa
     3181 ctttaaaatc taaagaaggc aaaatgattg agatcagacg ttatttacat cagcatccag
     3241 aattatcttt tcatgaagat gaaacggcga aatacatcgc tgaattttac aaaggtaaag
     3301 atgtggaagt agaaacgaat gtcggaccac gtggaattaa agtaacgatt gattcaggga
     3361 aacctggtaa aacattagca atccgtgcag actttgacgc attacccatt actgaagata
     3421 caggattatc ttttgcatca caaaataaag gtgttatgca cgcatgtggt cacgatgcac
     3481 atacagcata catgcttgta ttagcagaga cgcttgctga aatgaaagat agttttacag
     3541 gaaaagtcgt tgtgatacat caaccagctg aagaagtacc accaggtggt gctaaaacaa
     3601 tgattgaaaa tggtgtatta gacggtgttg atcatgtatt aggtgtacac gtcatgagca
     3661 caatgaaaac aggtaaagtg tattacagac ctggttatgt tcaaacagga cgcgcattct
     3721 tcaaattgaa agttcaaggt aaaggtggtc atggttcatc accacatatg gccaatgatg
     3781 ccattgttgc aggtagctac ttcgtcacag cgttacaaac agttgtatct agacgactaa
     3841 gtccatttga aaccggtgtt gtcacaatcg gttcatttga cggtaaaggt caattcaatg
     3901 tcattaaaga tgttgttgaa attgaaggtg atgtacgtgg attaacagat gctacaaaag
     3961 caacaattga aaaagaaatt aaacgtttat caaaaggatt agaggatatg tatggtgtaa
     4021 cttgcacctt agaatataac gatgattatc cagcattata taatgatcca gagtttactg
     4081 agtacgtggc taagacgttg aaagaagcaa accttgattt tggtgttgaa atgtgtgaac
     4141 cacaaccacc ttcagaagac tttgcatatt atgctaaaga acgcccaagt gcctttattt
     4201 atacaggtgc agcggtggaa aatggtgaaa tttacccaca tcatcatcct aaatttaaca
     4261 tttcagaaaa atcattactt atttctgcag aagcagtagg gacagttgtt ttagattacc
     4321 ttaaaggaga taactaacat gaatgaaacg tatcgcgggg gcaacaagtt aatcttaggt
     4381 attgtattag gtgttattac attttggttg tttgcacaat cacttgtaaa tgttgtaccg
     4441 aatttacaac aaagttttgg tacagacatg gggacaatta gtattgcggt cagtctaact
     4501 gcactatttt caggcatgtt tgttgttgga gcaggtggtc tggcagataa aattgggcgc
     4561 gtgaaaatga cgaatatcgg tttattatta agtattattg gttcagcatt aattattatt
     4621 acgaatttac cggcattatt aattttaggt cgtgttatac aaggtgtatc agcagcgtgt
     4681 attatgcctt ctacattggc cattatgaaa acttattatc agggtgctga acgtcagcgt
     4741 gccttaagtt attggtctat cggttcttgg ggtggcagtg gtatctgttc actcttcggt
     4801 ggtgcagttg cgacaactat gggttggaga tggattttca tcttctcaat tatcgttgcc
     4861 gtactttcaa tgttactcat caaagggacg cctgaaacga aatcagaaat taccaataca
     4921 cataaatttg acgttgcagg gctaattgtt ctagtagtta tgttgctaag tttaaacgtt
     4981 gtcattacta aaggtgcagc acttggttac acatcattat ggttctttgg tttgattgca
     5041 atcgtaattg tagcattctt tattttctta aatgttgaga aaaaagtaga taatccactt
     5101 attgatttta aattatttga aaataaacca tatacaggtg caacgatttc gaacttctta
     5161 ttaaacggtg ttgcaggtac attaattgta gcgaatacat tcgtgcaaca aggtttaggt
     5221 tatacagcat tgcaggcagg atacttatca attacttatt taatcatggt gttattgatg
     5281 attcgagttg gtgaaaaatt attacaaaaa atgggttcta agcgaccaat gttattaggt
     5341 acattcattg tggtcattgg tattgcactt atttcattag tattcttacc aggcatattt
     5401 tatgttatca gttgtgtcgt aggatattta tgtttcggac taggcttagg tatttatgca
     5461 acaccttcta cagatacagc tatttcgaat gcaccgttag ataaagttgg cgttgcttca
     5521 ggtatttata aaatggcttc atcacttggt ggcgcattcg gtgtcgcaat tagtggtgct
     5581 gtatatgctg gtgcagttgc tgcaacgagc attcatacag gtgcgatgat tgcactttgg
     5641 gttaacgtat taatgggaat catggcattt atcgcaattt tattcgcgat tcctaatgat
     5701 gataaacgtg tcaaagatgc gaaataataa cgatgacacg ccaagtgttt tgataatgat
     5761 ataaatgaaa gtaaagcaaa ttaaatgagg aaatgtatag agtagtgcat atagataaat
     5821 ctatgattgg attaacacta atataaatat aaatgaaaat gaaaaaccat ccacttaaat
     5881 ttttgataag ttaggtggat ggttatttat tgcgttgttc taaaaatttg gacatttata
     5941 tggaaagaac tatatataat gtgaaggtta tggcaattcg cttcaatgaa ggcgacgtgt
     6001 aaaagtgtat attaaacgtt tcggggtcag actccgagaa cagtgtccgt catgcttacc
     6061 gcatactaat gaagcattat tatatcagtg tttatagccg atttaagata ataaattttg
     6121 ctaaacaaaa aagccaaccc atgaatgttg gattggcttt ttacatgcat ctgaatctct
     6181 aattttaaaa aaatatgaat ataaataaga cagtaaaaat taaatttcag ttgttgcaat
     6241 ttcttcatct gtaggtacat catcgttaag gccaacaagt gcttcagaaa catttcgtga
     6301 atgataaccg atacgttcaa gaacaccaat catatcgata tatagtaatc cgccttttgt
     6361 tgtacattca ccacgattaa ggcgtttaat atgacctttg cgtagtttat gttcaatatt
     6421 aaatgattct ctactacgtt ctacaatttc atcttttttc gttttgtcat aaacatctaa
     6481 catgtcgatg gctttatcaa atgactcagc aacatggttg aataatttat ccataccgcg
     6541 ttgtgcatct tctgtaatgc gaatatcttc atcatgttgg cgttttaatt gagcgacata
     6601 ctcttctgtt agctctgcta cttttaaaat agagcgattg acatcaaaca taactgctaa
     6661 acgctcaacg tctgccttcg taatggcttt tgtagaaatt ctaactaaat aatttcgaat
     6721 gctatcattg attgtttcaa cagcttgatg cttttgttca agctttttga tcaatttttt
     6781 atcgtctttt gtaatttcgc gaatgtcttc aaacattgat aagacaatct gacccacatt
     6841 ttgtaattct ttttgagttt cttgtaatgc aacaccaggt gcgtgataaa caagatcttt
     6901 gtttaagtgc tgaggtttat agtcatcagc aatatcttta cctgggacaa gctttgtaac
     6961 aatccatgct aaacctgcta caaatggtaa ttgaatcaaa gtatttgtta tgttgaagat
     7021 accatgtgat actgcaatcg tcatcgctgg ttttaagtgc cataaatctt gtaacaaact
     7081 aatcaaatga atcacaactg gcaagaaaat tgtgaagata attaccccga ttaagttaaa
     7141 gatgacgtgt acaagcgccg cacgttttgc agcgattgag ccggctaaac tagctaagat
     7201 agctgtaatc gtggtaccaa tgttatcgcc tagtaacaca gggattgctg cgtttaagct
     7261 aattaaatct tgttgataaa attcttgtaa aataccaatc gtcgcacttg aactttgaac
     7321 tagtgctgtt aaccctgcgc cgacaatgac agcgagtatt ggatttgtag acatatcaag
     7381 cattaattgc ttaaatccat ctaatgatgc taaaggttta acggcatcac ccataaattc
     7441 tagaccgaag aatagtgaac cgaaaccgaa tagtatgcgg ccaatgttat tgattttaga
     7501 gcgtttaaag aaaaagatta agaatgcacc taatgctaaa attggcattg catattcgcc
     7561 taaatctata ccgataataa atgcagttac cgttgttccg atattagcac ccattatcac
     7621 tccaatggct tgtttcaatg tcataaatcc agctgttacc agtccgattg tgataactgt
     7681 cgtacctgaa ctactttgta ttaaaatagt tacaacgata cctgcaataa cacctaatac
     7741 tggatttgat gtaaatttgt ttaaaatatc tcgtagcctg tctcctgctg atgcttgaag
     7801 cccgtctccc atgattttta agccgtaaag gaaaatacct aaaccaccta aaaaggagaa
     7861 aatgacttct gtaaccgaca tttccattat ttcacctcaa ataagcttta tatttagatt
     7921 atcgcttata attgtaaatt taatgttaag attaggtaaa attatttaac aatatatgtt
     7981 atttgtatat gacttgtaaa atatcgtcac ttattatgta aattttcagt gtgaaatggc
     8041 aggtttgcaa tcacttgttt aacaaaatga tgcaatcaat catgtaatta tgtttcatca
     8101 aaaaaatcat gagagtggaa caacgaaata atttttatga aaacatcatt tctgtcccag
     8161 tctcatgatt tgaaatcacc aaataaaaat ctattaatgg ttttcgttat aacaatttgt
     8221 gttcttttaa taatgactca atgtacgtac cttttatctt tttaaggaat cctgctaatg
     8281 cgagtttttg cattttcgaa tctttagtaa tctcacgcaa atcttggtgg tcattcagtt
     8341 cgtatatggc atccattaag acgcgaagat caaatggact attgatgact tctggaatac
     8401 cacgatctat atttagtaat tgataaacag cttccatggc agtacgaacc gaatattctg
     8461 ttgtaaatac agtgtctcgc tctgtttctg caaagttacc aataaatgct aagttctgag
     8521 attgatgcgg gacgactaaa ggtctgtcgc cgatagcacg cgtcatgaaa taagatgtga
     8581 tatatggcat ataaacagga atcgtattag atgcatgttt tgctaagtct tcaattttgt
     8641 cagttgatac acctaagtga tacagccatt cttggcatat ttcattacca ctacattctg
     8701 taattggctt tttaatataa tcgccgttta catctgaata taaggcataa atccatgtag
     8761 atatttcatt ttcaggttgg tctttaaact gttgctgacg attgattgta aaactcattt
     8821 gccatgcaga atcattgatt gtaataatac cgcctgtaac tgttttgcct gcaagtgggt
     8881 cacgtttaca aatactttct attgtatcga taatctcttt attgtttgtt gtagaagttg
     8941 ctgaaacaaa ccaacttttt ttaggaatat tttggcaaaa cttatcagga ttaccaaatt
     9001 caggactttg tcgcgctaaa tttttccata gtgtccaact accacctaat tcgtcagttg
     9061 gtggcgctgg tgtatcatta tcaccataag tagagctttc tgtaatacta ccgtttgtca
     9121 caaagacaag atcgtttata gtcagtttaa tagattctgc attaccatta cggtcaatta
     9181 atatttctcg ggcaattttt tgacttgtcg taacatctat tttaatatct tcgactttta
     9241 catcgtattc aaattgaacc ccatgcgatt ttaaatattc aaccataggt aatactaaag
     9301 attcatattg attatattta gtgaatttta aagctgaaaa gtctgcgaga ccactaatat
     9361 gatgaacgaa tcgcattaga tagcgacgca tttccattgc agaatgccac ggttcaaatg
     9421 caaacatcgt tttccagtaa atccaaaagt ttgaattaaa gaagtcatcg gaaaatacat
     9481 ctgttatttt gacatcatct aaatcttctt cattcgttaa gcataaatct aaaatttctt
     9541 taatcgccgt tttagtcaaa gtgaagtctc cgtctgtgac taaacgttga ccctgtttct
     9601 caataacacg acagcgagaa tagttagggt cttctttgtt tagccaatag aactcatcta
     9661 atacagacgc gttatcgatt tctaatgaag ggatagatct gaataagtcc cacaaacatt
     9721 caaagtggtt ctccatttca cgaccaccgc ggacaacata gccttttaaa ggcatatttt
     9781 caccatcaag actaccacct gctttaggta actcttctaa aatatgaatc ttcgaacctt
     9841 ccatttgacc atcccttatt aaaaaacaag ctgcagcaag tgaagctaga ccagatccga
     9901 ttaagtaagc ggatttgttt tctacatttt caggtttttt agggcgcgca aatgcttcat
     9961 aatttccata actgtaatac atatccacca agtcctttcg tattagaata cactcagact
    10021 ataccccttt gtgaattgca aatattagta tttatattgg taaatttgac gaaattagat
    10081 catttttata tttttgattt ttaaaatttt atcactcgaa tgtttttgga ttctgaggtg
    10141 cgggacttgg aataattatg tattttgaaa gaagttactt aaactaacgc ctagtagagt
    10201 gataattccg cctataatag caagggttgt tggtagctcg tctaataaca gataagataa
    10261 taataaagaa acgataggtg ttaaataaag agacattgtt gcatcagaga caccaactga
    10321 cttcacaata taagcaagca aaacgtatgg aattatagta gggaatatag ctaaataaag
    10381 taccgatact attgatgtaa aagtggcgcc gtgtatatcg ttgatgattt caggaataaa
    10441 aataagcata aatggtgagc ttgccattat tgtatatagt gtgaaagcga tgaagccgta
    10501 tttttctatg tattttttct ggaaagtaaa atacaaactt tcactaaaag atgcaagtaa
    10561 aataataaaa acacctaata cattaatagt tgtgtaatca tctttactta ttgaaataat
    10621 ggatattcct ataaatgcga cgagtgaact taaccaattc cattttgaaa aatgctcttt
    10681 taaaaatata taagctaaag cactagaaaa aataggcgtt gtagagacta gaattccaga
    10741 tatacctgca ctaatcaaag cttcaccaaa atttaaagct gtgtgatata tcacaaatcc
    10801 acaaaatcct aaaataaaaa taacagggat atctcttagt tcaggggtag gcaatttctt
    10861 tataattacg aacggcaaga gaattattgt tgctaaaatt aaacgaaatg ccgacaatga
    10921 ttctgcacta aaatcattta acgcaatctt tatcattgga aatgcagatc cccacaatat
    10981 gatagtaaat aaatatgata gaaaagtagt gtcttgaagt ttattcatta atatcatcac
    11041 tcctttaatt atgtgtttct atattaaaaa atatgattta aaatgagtac aaccaatttt
    11101 gaatggattt acctatccaa ttttaaaagg gagggagaag atggctaaat ataaagatat
    11161 tgctagtgac ataagagata aaataatcac aggggattgg ttttatggaa tgaaaatacc
    11221 ttcacatagg cagttggcga tacagtacaa cgtaaataga gtaacgatta ttaaaagtat
    11281 tgagttatta gaagctgaag gatttatcta tactaaagta ggtagtggaa catatgttaa
    11341 tgactatttg aatgaagcac atattacaaa taagtggtct gaaatgatgt tatggtcctc
    11401 tcaacaaaga agtcagtata cggtgcaatt aattaataaa attgagacag atgattcgta
    11461 tatacatata agtaaaggtg aattgggtat atcgttaatg ccacatattc aattgaaaaa
    11521 agccatgtct aatacagcca gtcatattga agacttatct tttggttata ataatggcta
    11581 tggttatatc aagttaagag atattatcgt tgaacgaatg tcaaagcaag gtataaatgt
    11641 aggtagagaa aatgtaatga tcacttcagg cgctttacat gccattcaac ttttatctat
    11701 tgggttttta ggtcaagatg ccataataat ttcgaataca ccatcatata ttcactctac
    11761 aaatgttttt gagcaattga attttagaca tattgatgtt ccttataatc aaattaatga
    11821 aattgatacc atcattgata gatttattaa ttttaaaaat aaagcgattt atatagaacc
    11881 taggtttaat aacccgacag gtcgttcttt aacgaatgag caaaagaaaa atataattac
    11941 ttatagcgaa agacataata ttcctatcat tgaagatgat atctttagag atattttctt
    12001 tagcgatcca actccttcta tcaaaactta tgataaattg ggaaaagtta tacatataag
    12061 cagtttttca aaaacgattg caccagcaat aagaataggt tggattgttg cttctgaaaa
    12121 aataatagag caattggcag atgtaagaat gcaaattgac tatggatcca gtattttgtc
    12181 acaaatggtt gtatatgaga tgttgaaaaa taagtcttat gataaacact tagtaaagtt
    12241 aaggtatgtt ttaaaagata aacgagactt tatgttaaac atcctcaata atttatttaa
    12301 ggatatagca cattgggagg ttccaagtgg aggttatttt gtatggttag tctttaaaat
    12361 agatatagat attaaatatt tattttacga attgttaagt aaagaaaaaa tattaatcaa
    12421 tccgggttac atttatggca gtaaagaaaa gagtataagg ctatcttttg cctttgaatc
    12481 aaatgaaaat attaagcatg cgctctataa aatttataca tatgtgaaaa aggtttaatt
    12541 aaaacaataa ttcgaatcat tatgtggcat gttaaacagc taaatataag ctatgcacat
    12601 ttaacaagcg gatactcctg acgcaattat agtatcatta catattgttt aatcgtttgg
    12661 ggtaatgtaa ttaaaaggta aaataggcag agtgttatac ataaaatgta aagaaagagg
    12721 cgatagaatg tctaaaatca ggtcttttac aatattaagt ctacttattt acttagctat
    12781 gatgtgctat acagtagtga cctatccaaa attaccaacc aaagtgccta ttcattataa
    12841 tttagcaggg gatgctgata attttgctga taaatgggtg ctgcttttga ttaatagcgc
    12901 atttatagtg atttggctta tatttttcat tgcaggtaga tactatgaac gatttgccaa
    12961 atggtcacat tataatcata caccacgtga aattcgagcg attaaattat ttttaagtac
    13021 gttaaattta gagattatga gctatatgtc tatcttcaca gtattagaaa tttggcaaat
    13081 acaacaccat catcaattca atttactatg gtttaatatg atatttatca ttatcattgg
    13141 tttgacgctt gtcatatttt gtttacttcc tacaattcat aaaatgagag attctcaata
    13201 aaaatataaa ctctcatgat gcagttttga agcgaattcc tgagattatt ataaataata
    13261 tctagtattt ataattaaaa tgatatatta tttattatag ttaaatattg tgtatatttt
    13321 gtgaactttt tgtaaaaatt cgattgcctg tcacatatag gagtgttaca ttttaaatat
    13381 gtgatcatcg caaaatataa gttgaaatag gttgtagatt aatcagaatg ataaatattt
    13441 tatataaaga gagggagtca ttatgacact acttactgta aatccattcg ataatgtcgg
    13501 attatcagcc ttagttgcag cagtacctat tattttattt ttattatgct taaccgtttt
    13561 taaaatgaaa ggcatttatg cagcattgac aactttggtt gttacattga ttgtggcttt
    13621 atttgtattt gaattaccag cgcgtgtatc agcaggtgcg attacagaag gcgttgttgc
    13681 cggtattttc ccaataggat atatcgtttt aatggcagtt tggttatata aagtttctat
    13741 taaaacagga caattttcta ttattcaaga tagtattgca agtatttcag tggaccaaag
    13801 aatccaacta ttattaattg gattttgttt caacgcattt ttagaaggtg cagcaggatt
    13861 tggtgtgcca attgcgattt gtgcagtatt attaattcaa cttggatttg aaccattaaa
    13921 agcagcgatg ttatgtttaa ttgctaatgg tgcggcgggt gcctttggtg caattggttt
    13981 accagttagt attattgata cgtttaactt aagtggaggc gttacaacat tagatgttgc
    14041 gagatactca gcattaacac ttccaatttt aaactttatt attccatttg ttttagtatt
    14101 cattgtagat ggtatgaaag gtattaaaga aattttacct gtcattttaa cagtgagtgg
    14161 tacatatact ggattacaat tattattaac aatattccat ggtccagaac tagcagacat
    14221 tattccatca ctagcaacaa tggtggtgtt agcatttgtt tgtcgtaaat ttaaaccgaa
    14281 aaacattttc agattggaag cgtctgaaca taaaattcaa aaacgaacgc ctaaagaaat
    14341 tgtctttgct tggagtccgt tcgtaatttt aactgccttt gtattagtat ggagtgcacc
    14401 attcttcaaa aaattattcc aacctggagg tgcacttgaa agtttagtaa taaaattgcc
    14461 aattccaaat actgtgagtg atttatcgcc taaaggaatt gcgttgcgtc tcgatttaat
    14521 tggtgcaact gggacagcga ttttattaac agtaattatt acaattttaa ttacgaagtt
    14581 aaaatggaaa agtgcaggtg ctttattggt cgaagcaatt aaagaattat ggttaccgat
    14641 ccttacaatt tcagctatcc tagctattgc taaagttatg acatacggtg gtttgactgt
    14701 agcaattgga caaggtattg ctaaagcggg agcaattttc ccattattct ctccagtatt
    14761 aggttggatt ggtgtgttta tgactggttc agttgtaaat aacaatactt tattcgcacc
    14821 tattcaagcg acagtggcac aacaaatttc aacaagcggt tcattacttg tggcagctaa
    14881 cactgcaggt ggtgtagcag cgaaacttat ttcaccacaa tcaattgcca ttgcgactgc
    14941 agctgttaaa aaagttggtg aagaatctgc attattaaaa atgacgttaa aatacagtat
    15001 tatatttgtt gcttttattt gtgtttggac gtttatacta acgttaatat tctaaatata
    15061 aataatgttg tcacttggat tcaaatgaca ttttaaatct aattattcat gaatcgaact
    15121 agtacgaaat gcaatgagca tcttgtctag ttcgattttt taatgcctaa aaatgtcata
    15181 tatgtaatca gagtagaaag tgttgaggcg tttcagaagt tgtttagaaa agtaagtaaa
    15241 ataaaaaatg cactgagcaa caaaagatgt tgctcgtgca tttagatgat tcttatcatt
    15301 tcaaataaga atgtgttaat caacgtatat aagttaaaat tggtttggat aaaatgatat
    15361 ctatcgttgt gtattgtttg tttttatagt tcgcgacgac gtccagctaa taacgctgca
    15421 cctaaggcta atgataatcc accaaataca gttgtaccga tgaatggatt ttcttcacca
    15481 gtttctggta atgcttgagc tttgttagca tctgcatggt ttgctggttg cttcttatca
    15541 acaacaagtt cttgaccagg tttgatcatg tttttatcag ctaatttgtt atctgcagca
    15601 attttgtcag cagtagtgcc gtttgctttt gcaatgtcat ttactgtatc accaggttta
    15661 acgacatgta ctccgttacc atcttcttta ccaggtttgt tgccatcttc tttgccaggc
    15721 ttgttgccgt cttctttacc aggttttttg ttgtcttctt taccaggctt gttgccatct
    15781 tctttaccag gttttttgtt gtcttcttta ccaggcttgt tgccgtcttc tttgccaggc
    15841 ttgttgttgt cttctttacc aggcttgttg ttgtcttctt tgccaggctt gttattgtct
    15901 tctttgccag gcttgttatt gtcttcctct tttggtgctt gagcatcgtt tagcttttta
    15961 gcttctgcta aaatttcttt gctcactgaa ggatcgtctt taaggctttg gatgaagccg
    16021 ttacgttgtt cttcagttaa gttaggtaaa tgtaaaattt catagaaagc attttgttgt
    16081 tctttgttga atttgttgtc agcttttggt gcttgagcat catttagctt tttagcttct
    16141 gctaaaaggt tagcgctttg gcttgggtca tcttttaggc tttggatgaa accattgcgt
    16201 tgttcttcgt ttaagttagg taaatgtaag atttcataga aagcattttg ttgttctttg
    16261 ttgaatttgt tatccgcttt cggtgcttga gattcattta actttttagc ttctgacaat
    16321 aggttagcac tttggcttgg gtcatctttt aagctttgga tgaaaccatt gcgttgttct
    16381 tcgtttaagt taggcatatt caagatttca tagaaagcat tttgttgttc tttgttgaaa
    16441 ttgttatcag ctttcggtgc ttgagattcg tttaattttt tagcttcacc taaaacgtta
    16501 gtgctttggc ttgggtcgtc tttaagactt tgaatgaagc cgttacgttg cgcttcgttt
    16561 aagttaggca tgttcaagat ttcatagaag gcgctttgtt gatctttgtt gaagttattt
    16621 tgttgcgcat cagcttttgg agcttgagag tcattaagtt tttgagcttc acctaaaacg
    16681 ttagcacttt ggcttggatc atctttaagg ctttggataa aaccattgcg ttgatcagca
    16741 tttaagttag gcatatttaa gacttgataa aaagcatttt gttgagcttc atcgtgttgc
    16801 gcagcatttg cagcaggtgt tacgccacca gatataagta atgtacctaa agttacagat
    16861 gcaataccta cacctagttt acgaattgaa taaatgtttt tctttttcaa attaataccc
    16921 cctgtatgta tttgtaaagt catcataata taacgaatta tgtattgcaa tactaaaatc
    16981 tatatttata attaaattta aagg
//
LOCUS       DALAAR010000054        16271 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000054 DALAAR010000000
VERSION     DALAAR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.056"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            131..784
                     /locus_tag="O2458_001899"
     CDS             131..784
                     /locus_tag="O2458_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ3887761.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            818..1405
                     /locus_tag="O2458_001900"
     CDS             818..1405
                     /locus_tag="O2458_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ3887762.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1548..2441)
                     /locus_tag="O2458_001901"
     CDS             complement(1548..2441)
                     /locus_tag="O2458_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887763.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2689..2982
                     /gene="esxA"
                     /locus_tag="O2458_001902"
     CDS             2689..2982
                     /gene="esxA"
                     /locus_tag="O2458_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCZ3887764.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3066..6095
                     /gene="esaA"
                     /locus_tag="O2458_001903"
     CDS             3066..6095
                     /gene="esaA"
                     /locus_tag="O2458_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCZ3887765.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6095..6553
                     /gene="essA"
                     /locus_tag="O2458_001904"
     CDS             6095..6553
                     /gene="essA"
                     /locus_tag="O2458_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCZ3887766.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6525..6767
                     /gene="esaB"
                     /locus_tag="O2458_001905"
     CDS             6525..6767
                     /gene="esaB"
                     /locus_tag="O2458_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCZ3887767.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6780..8114
                     /gene="essB"
                     /locus_tag="O2458_001906"
     CDS             6780..8114
                     /gene="essB"
                     /locus_tag="O2458_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCZ3887768.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8136..12575
                     /gene="essC"
                     /locus_tag="O2458_001907"
     CDS             8136..12575
                     /gene="essC"
                     /locus_tag="O2458_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCZ3887769.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12605..12997
                     /gene="esaC"
                     /locus_tag="O2458_001908"
     CDS             12605..12997
                     /gene="esaC"
                     /locus_tag="O2458_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCZ3887770.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            13013..13327
                     /gene="esxB"
                     /locus_tag="O2458_001909"
     CDS             13013..13327
                     /gene="esxB"
                     /locus_tag="O2458_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCZ3887771.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13327..14001
                     /locus_tag="O2458_001910"
     CDS             13327..14001
                     /locus_tag="O2458_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCZ3887772.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            14001..14318
                     /locus_tag="O2458_001911"
     CDS             14001..14318
                     /locus_tag="O2458_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887773.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14328..16172
                     /locus_tag="O2458_001912"
     CDS             14328..16172
                     /locus_tag="O2458_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCZ3887774.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            16183..>16271
                     /locus_tag="O2458_001913"
     CDS             16183..>16271
                     /locus_tag="O2458_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCZ3887775.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKV"
BASE COUNT         6133 a         2223 c         3071 g         4844 t
ORIGIN      
        1 caaaaaatag tttgattaca taatgattct tgaaaaatgt tggttaactt aataatatgc
       61 atttttttgg cgaagaagat ttatttaact tataaaaata ttgaagtaag attggggaga
      121 ttatgaattt atggaattga aagtcgatga ttttgtaaag aatataaaaa gaccatactt
      181 gactgtattg ggagtatttg tagttgcagt ttcattattt tttgacgcga ttatgttttt
      241 ctacgctaaa ctatatgata aattgcctat gtatttactg gtgtttatgg cctttacagc
      301 tgtaattttg attatgatgt acatacaaga gaaaaatgaa aattacaaag ttgaaaaaag
      361 atatgtggtt agatatctca cacttaacgt tattgtggga tatactttgc cattgctttt
      421 tgtatctatt tacgtttttg gtgtagtcgg ttttggattt gatgttttca attattgtct
      481 aggtattatc ttgatgttat ttatttcttg gttaggttta tttttatttt ataaaaacga
      541 atttgatagt gaaaatccta atcctgcagt taatgccata gcaattatta taaaattatt
      601 tgcttttgga ggaatatttt atataagtct aatagttcct gtaacacagc aagaagagat
      661 ttttataggg ttaagtatat tcatcaatat aattgttgat gctcttcttg ttaggtcata
      721 ttttaattac gcgttatata agagtattaa gaaagatatc gaaaatgaag gtaagacaat
      781 gtaaaggaaa gacataaaaa ataataggag tctaaccatg ataattttta ttttaataac
      841 aatatttgct atttattata tagctatgat tgctagtttg tttaaaagtg aaggtttttc
      901 aataataggt ttaatattag atgttgttat aatggggaca ttgatttttt actattttat
      961 aggcgctcgc tttgttgatc atgatttaag caacttttta atgtttatgg atactggatc
     1021 atatattttt atgtactttg caattaagtg tttatgggtg aaacctaagg tagtaaatta
     1081 tttgattgca aaagaattgg gtgaatctaa agaggtcatt gaagagcagg aattagattt
     1141 acagacatca aagataagag gtatctactt tttcattatt tcagtagtga tgttaattat
     1201 cactaaatta agaatgcaac ctgagttaca ggcagatgct atatcgatga atcctgtatt
     1261 tatttttgtt ggtgttataa tcattttaat ttggctagta ctggatatat atcgcaagaa
     1321 aaaatacggt atattcttat tcaagacgat agtgccacta gttgttacga cttggattat
     1381 tatagctaca attatacttt cgtaaacgat tttgattaat ggggaatgta gagaacattt
     1441 ctttgtaatt ttataatcga ataacataca aaataaagcg accaattttg cagtgttacg
     1501 aattgtaaac tgcgaactgg tcgcttttta ttgatgtcat gattaattta atggatgtaa
     1561 ttatatgatg aaacttctga agcagagatg gttcttgatg aaacgatata ttcaccaatc
     1621 cagttcattt ctgaaattag aatacttcca tcaatattaa ctttttcaac gtaggctaca
     1681 tgaccaaatg gaccatttac tgtttgtaaa attgatcctc gtgttgggtg tctatctact
     1741 ttgaagccat tgcttgaagc ttggcctgcc cagtttttag catctcccca aaatgtacta
     1801 atcgtgtgtc catctttggc acgtttatca aagacatacc atgtacattg tccagcagta
     1861 tataagttgt tcttacttgt gataagaggc tgatcaatga ttttaccgtt acctaatgct
     1921 aaaggtttac cgtcagcggt ctttgctttg tcattaaatt cggcgatttg taattcgtca
     1981 tacaattcgt ctaaatcgat gcctgtaata agccctttgt tatcttttga aaaagcgtta
     2041 tttaattcat cgtcattgtc ttcgacattc ggtattgctg gtgtcaaagg attgcttggt
     2101 gacgtttgag gcggtgtgtg tgaatcaatt gcgtcattaa tgtgcgtata ctgaccactt
     2161 aatgaagaat ggtactgatt gttgttaaga tcacgttgat ttgcgtgttg atgattgtcg
     2221 tttgtacgtg actggttttg atgattgttg tttggcgtgt tgtttttgtc atatgtataa
     2281 gtatacgcgc cggtgtcttt attcactttg aactgtgcgt ttgggtgtgc tttctttgct
     2341 tcttctaatg ttttgctatc attcgtatat gcttgagccg agttaggcga catactaaat
     2401 aaagtaagag ttgtcatcgt cagtaaaatt gttttcttca taataaccat ttaatccttt
     2461 atgtatttaa tttaatttta gtatacacat ttatattaca aaaatgaatg gttaattaaa
     2521 aatatatggg tatattcaat atatttattt aaaaaaagct aaaaatactt aaaaaactat
     2581 atacatatac taataattta tatattattt gagtaaggag cactttttca aaaaatagtg
     2641 tccctaaaaa gttttgataa acttaaaata ttcaggaggt ttctagttat ggcaatgatt
     2701 aagatgagtc cagaggaaat cagagcaaaa tcgcaatctt acgggcaagg ttcagaccaa
     2761 atccgtcaaa ttttatctga tttaacacgt gcacaaggtg aaattgcagc gaactgggaa
     2821 ggtcaagctt tcagccgttt cgaagagcaa ttccaacaac ttagtcctaa agtagaaaaa
     2881 tttgcacaat tattagaaga aattaaacaa caattgaata gcactgctga tgccgttcaa
     2941 gaacaagacc aacaactttc taataatttc ggtttgcaat aagcattctg aaattggcaa
     3001 agtcacattt tctaatgtgg ctttgcttat cattttttta agaaaacaac tgaaaggaaa
     3061 taagcatgaa aaagaaaaat tggatttatg cattaattgt cactttaatt attataattg
     3121 ccatagttag tatgatattt tttgttcaaa caaaatatgg agatcaatca gaaaaaggat
     3181 cccaaagtgt aagtaataaa aataataaaa tacatatcgc aattgttaac gaggatcaac
     3241 caacgacata taacggtaaa aaagttgagc tgggtcaagc atttattaaa aggttagcaa
     3301 atgagaaaaa ctataaattt gaaacagtaa caagaaacgt tgctgagtct ggtttgaaaa
     3361 atggtggata ccaagtcatg attgttatcc cagaaaactt ttcaaaattg gcaatgcaat
     3421 tagacgctaa aacaccatcg aaaatatcgc tacagtataa aacagctgta ggacaaaaag
     3481 aagaagtagc taaaaacaca gaaaaagttg taagtaatgt acttaacgac tttaacaaaa
     3541 acttagtcga aatttattta acaagcatca ttgataattt acataatgca caaaaaaatg
     3601 ttggcgctat tatgacgcgt gaacatggtg tgaatagtaa attctcgaat tacttattaa
     3661 atccaattaa cgacttcccg gaattattta cagatacgct tgtaaattca atttctgcaa
     3721 acaaagacat tacaaaatgg ttccaaacat acaataaatc attattgagt gcgaattcag
     3781 atacgttcag agtgaacaca gattataatg tttcgacttt aattgaaaaa caaaattcat
     3841 tatttgacga gcacaataca gcgatggata aaatgttaca agattataaa tcgcaaaaag
     3901 atagcgtgga acttgataac tatatcaatg cattaaaaca gatggacagc caaattgatc
     3961 aacaatcaag tatgcaagat acaggtaaag aagaatataa acaaactgtt aaagaaaact
     4021 tagataaatt aagagaaatc attcaatcac aagagtcacc attttcaaaa ggtatgattg
     4081 aagactatcg taagcaatta acagaatcac tgcaagatga gcttgcaaat aacaaagact
     4141 tacaagatgc gctaaatagc attaaaatga acaatgctca attcgctgaa aacttagaga
     4201 aacaacttca tgatgatatt gtcaaagaac ctgatacaga tacaacattt atctataaca
     4261 tgtctaaaca agactttata gctgcaggtt taaatgagga tgaagctaat aaatacgaag
     4321 caattgtcaa agaagcaaaa cgttataaaa atgaatataa tttgaaaaaa ccgttagcag
     4381 aacacattaa tttaacagat tacgataacc aagttgcgca agacacaagt agtttgatta
     4441 atgatggtgt caaagtgcaa cgtactgaaa cgattaaaag taatgatatt aatcaattaa
     4501 ctgttgcaac agatcctcat tttaattttg aaggcgacat taaaattaat ggtaaaaaat
     4561 atgacattaa ggatcaaagt gttcaactcg atacatctaa caaggaatat aaagttgaag
     4621 tcaatggcgt tgctaaattg aaaaaggatg ctgagaaaga tttcttaaaa gataaaacaa
     4681 tgcatttaca attgttattt ggacaagcaa atcgtcaaga tgaaccaaat gataagaaag
     4741 caacgagtgt tgtggatgta acattgaatc ataaccttga tggtcgctta tcgaaagatg
     4801 cattaagcca gcaattgagt gcattatcta ggtttgatgc gcattataaa atgtacacag
     4861 atacaaaagg cagagaagat aaaccattcg acaacaaacg tttaattgat atgatggttg
     4921 accaagttat caatgacatg gaaagtttca aagacgataa agtagctgtg ttacatcaaa
     4981 ttgattcaat ggaagaaaac tcagacaaac tgattgatga cattttaaat aacaaaaaga
     5041 atacaacaaa aaataaagaa gatatttcca agctgattga tcagttagaa aacgttaaaa
     5101 agacttttgc tgaagagcca caagaaccaa aaattgataa aggcaaaaat gatgaattta
     5161 atacgatgtc ttcaaattta gataaagaaa ttagtagaat ttctgaaaag agtacgcaat
     5221 tgctatcaga tacacaagaa tcaaaatcaa ttgcagattc tgttagtggc caattaaatc
     5281 aagtcgacaa taatgtgaat aagctacatg cgacaggtcg agcattaggc gtaagagcta
     5341 acgatttgaa tcgtcaaatg gctaaaaacg ataaagataa tgagttgttc gctaaagaat
     5401 ttaaaaaagt attacaaaat tctaaagatg gcgacagaca aaaccaagca ttaaaagcat
     5461 ttatgagtaa tccggttcaa aagaaaaact tagaaaatgt tttagctaat aatggtaata
     5521 cagacgtgat ttcaccgaca ttattcgtat tattgatgta tttactatca atgattacag
     5581 catatatttt ctatagttat gaacgtgcca aaggacaaat gaatttcatt aaagatgatt
     5641 atagtagtaa aaaccatctt tggaataatg tcattacgtc aggtgttatt ggtacaactg
     5701 gtttggtaga agggttaatt gtcggtttaa ttgcaatgaa taagttccat gtattagctg
     5761 gctatagagc gaaattcatc ttaatggtga ttttaactat gatggtcttc gtacttatta
     5821 atacgtattt actaagacag gtaaaatcta tcggtatgtt cttaatgatt gctgcattgg
     5881 gtctatactt tgtagctatg aataatttga aagcagctgg acaaggtgtg actaataaaa
     5941 tttcaccatt gtcttatatc gataacatgt tcttcaatta tttaaatgca gagcatccta
     6001 taggcttggt gctagtaata ttaacagtac ttgtgattat tggctttgta ctgaacatgt
     6061 ttataaaaca ctttaagaaa gagagattaa tctaatgttg atgaatagcg tgattgcttt
     6121 aactttttta acagcatcta gcaataatgg cggacttaat attgatgtgc aacaagaaga
     6181 ggaaaagcga atcaataatg atttaaatca atatgataca acgctattta ataaagacag
     6241 caaagcggtt aatgatgcga ttgctaagca gaaaaaagaa cgacaacaac aaataaaaaa
     6301 tgatatgttt caaaatcaag cgagtcactc gactcgcttg aatgaaacta aaaaagtgtt
     6361 attttccaaa tctaacttag aaaagacttc ggagagtgat aaaagcccct atattcaaaa
     6421 caagcaggag aaaaaaatat tcccgtacat tttgatgtct gtaggggctt ttttgacttt
     6481 aggatttgtc attttttcaa ttcataaagg gagacgaacg aaaaatgaat cagcacgtaa
     6541 aagtaacatt tgattttact aattataatt acggcacata tgacttagca gtaccagcat
     6601 atttaccgat aaaaaactta atagctttag tattggatag tttggacatt tcaatatttg
     6661 atgtcaatac acaaattaaa gtgatgacga aaggtcaatt acttgttgaa aatgatcgac
     6721 tcattgatta tcaaatcgct gatggagata ttttgaagtt actataggag gaaaaataga
     6781 tggttaaaaa tcataaccct aaaaatgaaa tgcaagatat gttaacgcct ttagatgctg
     6841 aagaagcagc taaaacaaaa ttacgcttag atatgagaga gattcctaag tcttcaatta
     6901 aaccagaaca ttttcattta atgtacttat tagaacaaca ttctccatat tttatagatg
     6961 ctgaattaac tgaactacgt gacagtttcc aaatacatta tgacattaat gacaatcata
     7021 caccttttga taatattaaa tcatttacta aaaatgaaaa attacgttac ttactcaata
     7081 tcaaaaattt agaagaagta aatcgtacac gctacacatt tgtgttggca ccagatgaat
     7141 tatttttcac aagagatgga ttacccattg ctaaaacaag agggttacaa aatgttgttg
     7201 atccattacc tgtgtcagaa gctgaatttt taacaagata taaagcgctg gttatctgtg
     7261 cattcaatga gaaacaatca tttgatgctt tagttgaagg aaacttagaa ctacataaag
     7321 gaacgccatt tgaaactaaa gttattgaag cggcaacgtt agatttacta acggcatttt
     7381 tagatgaaca gtatcagaaa caagaacaag attatagtca aaattatgca tatgtacgca
     7441 aagtaggaca taccgttttc aaatgggttg ctatcggtat gacaacgtta agtgttttat
     7501 taattgcatt cttagccttt ttatattttt cagtaatgaa gcataatgag cgcattgaaa
     7561 aaggatacca agcatttgta aaggatgatt atacgcaagt actaaatacg tatgatgatt
     7621 tagatggtaa aaaattagat aaagaggcac tttacattta tgccaaaagt tatatccaaa
     7681 caaataaaca aggtttagaa aaagataaga aagaaaattt acttaataat gtgacaccaa
     7741 attcaaacaa agactactta ttatattgga tggaattagg acaaggacat cttgatgaag
     7801 cgattaatat tgccacttat ttagatgata acgatattac aaagttagcg ttgattaata
     7861 aattaaatga gattaaaaat aacggagatt tatcgaatga taaacgttct gaagaaacga
     7921 aaaagtataa cgataaattg caagatattt tagacaaaga aaaacaagtt aaagatgaaa
     7981 aagcgaaatc tgaagaagag aaagcaaaag cgaaagatga gaaattaaag caacaagaag
     8041 agaacgaaaa gaaacaaaaa gaacaagcac aaaaagataa agaaaaacgc caagaagctg
     8101 aaagaaaaaa atagtatagg actgaggcaa agacaatgca taaattgatt ataaaatata
     8161 acaaacaatt gaagatgctc aatttgcgag atggtaagac atatactatt agcgaagacg
     8221 agcgtgcaga tattacgttg aaatcgttag gcgaagtcat tcatttagaa caaaataatc
     8281 aaggtacttg gcaagcgaat catacttcta ttaataaggt gcttgttaga aaaggtgacc
     8341 ttgatgacat tacattacag ctttatacag aagctgatta tgcatcattt gcgtatcctt
     8401 caattcaaga tacgatgaca attggaccaa atgcgtatga tgatatggtt attcaaagct
     8461 tgatgaatgc catcattatt aaagattttc aatcaataca agaatcacaa tacgtacgca
     8521 ttgtgcacga taaaaataca gatgtgtata ttaactatga actacaagag caactaacga
     8581 acaaagctta cattggtgat catatttatg ttgaagggat atggctcgaa gtacaagctg
     8641 atggtttaaa tgtattgagt cagaatacag tggcatcgtc attaattcgc ttaacacaag
     8701 agatgccaca tgcacaggca gatgattaca atacgtacca tcgttcgcca aggattattc
     8761 accgtgaacc gacggatgat attaagattg aaagaccgcc acagccaata cagaagaaca
     8821 atacagtgat atggcgttcc attataccgc cattagtaat gattgcttta actgttgtca
     8881 tctttttagt gagaccaatt ggtatttata ttttaatgat gattggtatg agtacagtaa
     8941 cgatagtatt tggtattaca acgtatttct ctgaaaagaa aaagtataac aaagatgttg
     9001 aaaaacgaga gaaagattac aaagcttatt tggataataa atctaaagaa attaacaaag
     9061 cgattaaagc acaacgtttt agtttgaatt accattatcc aacggttgct gaaattaaag
     9121 atatcgttga aacgaaagca ccaagaatat atgaaaaaac atcgcatcat cacgatttct
     9181 tacattataa gttaggtatt gcgaatgtag aaaagtcatt caaattagat taccaagaag
     9241 aagaatttaa ccaacgtcgt gatgaactat tcgacgatgc taaagaattg tatgaatttt
     9301 acacagatgt agaacaagca ccattaatca atgatttaaa tcatgggcca attgcatata
     9361 ttggtgcacg acatctcatt ttagaagaat tggagaaaat gctaattcaa ttgtcaacat
     9421 tccatagtta tcatgattta gagtttctat ttgtgacacg tgaagatgaa gttgaaacat
     9481 tgaaatgggc acgttggttg ccacatatga cattgagagg gcaaaacatt agaggatttg
     9541 tttacaatca acgaacgcgt gaccaaattt taacgtcaat ttatagcatg attaaagaac
     9601 gtatccaagc tgtgcgtgaa cgcagcagaa gtaatgagca aattattttc acaccgcaat
     9661 tagtgtttgt cattacagat atgtcattaa ttattgatca tgtcatttta gaatatgtaa
     9721 accaagattt atcagaatat ggtatttcat taatctttgt tgaagatgtg attgaaagtt
     9781 tgccagagca tgtagatacc attattgata tcaagtctcg tactgaaggc gaactgatta
     9841 cgaaagaaaa agaattagtt caattgaaat ttacacctga aaatattgat aacgtcgata
     9901 aagaatatat cgcgcgacgt ttggcgaatt tgatacacgt cgaacatttg aaaaatgcaa
     9961 ttcctgatag tattacattt ttagagatgt ataacgtgaa agaagtagat cagcttgatg
    10021 tggttaatcg atggagacaa aacgaaacat acaaaacgat ggcagtacct ttaggtgtaa
    10081 gaggtaaaga tgatatttta tcattgaact tacatgaaaa agcacacggg ccacatggtt
    10141 tagttgctgg taccactggt tcagggaaat ctgagattat ccaatcatac attttatctt
    10201 tagctattaa ttttcaccct catgaagttg cattcctatt gattgactat aaaggtgggg
    10261 gtatggcgaa cttatttaaa gatttagtcc atttagttgg tacgattaca aacttagatg
    10321 gcgatgaagc gatgcgtgcc ttaacatcaa tcaaagccga attgagaaaa cgtcaacgtt
    10381 tattcggaga gcatgatgtt aaccatatta atcaatacca taagttattt aaagaaggta
    10441 ttgcgacaga accaatgcca catttattca ttatttccga tgagtttgcc gaattaaaat
    10501 cagaacaacc tgattttatg aaagaacttg tatcaacggc acgtattgga cgttcgttag
    10561 gtattcattt aatacttgcg acacaaaaac catcgggtgt tgttgatgac caaatttggt
    10621 ctaactctaa atttaagttg gcattaaaag tacaagatag acaagacagt aatgaaattt
    10681 taaaaacacc agatgcagca gacattacat taccaggtcg tgcgtattta caagttggta
    10741 ataatgaaat ttatgaatta ttccaatctg catggagtgg tgcaacatat gacatcgaag
    10801 gcgataaatt agaagttgaa gataagacga tttacatgat taatgactat ggtcaacttc
    10861 aagcaatcaa caaagacttg agtggacttg aagatgaaga aacgaaagaa aatcaaactg
    10921 agttagaagc ggtcatagat catatcgaat ctattacaac acgattagaa atcgaagaag
    10981 ttaagcgtcc atggctacca ccattgccag aaaatgtata tcaagaagat ttagtagaaa
    11041 cagatttcag aaaattatgg tcagatgatg caaaagaagt ggaattaaca ttaggactta
    11101 aagacgtacc agaagaacaa tatcaaggac cgatggtatt gcaattgaaa aaagctgggc
    11161 acatcgcgtt aatcggaagt ccaggatatg gtagaacaac gttcttacac aacattattt
    11221 tcgatgttgc aagacaccat cgtcctgatc aagcacacat gtacttgttc gatttcggta
    11281 ccaatggttt gatgccagtt acagacatac cacatgtcgc tgattacttt acagtagatc
    11341 aagaagacaa gattgctaag gcgatacgta tatttaatga tgaaattgat cgtcgtaaga
    11401 agattttaag tcagtatcgt gtcactagta tttctgaata tcgaaaatta actggtgaaa
    11461 caattccgca tgtctttatt cttattgata actttgacgc agtaaaagat tcacctttcc
    11521 aagaagtttt tgaaaatatg atgattaaaa tgacgcgtga agggctagca ttagacatgc
    11581 aagtaacctt aactgcttca agagctaacg ctatgaaaac accaatgtac attaatatga
    11641 aaacgcgtat cgcaatgttt ttatatgata aatcagaggt gtcgaacgta gtaggacagc
    11701 aaaaatttgc ggttaaagat gttgtgggtc gagcattgtt aagtagtgat gacaacgtat
    11761 cattccatat tggccaacca tttaaacatg atgagaccaa atcatataat gatcaaatta
    11821 atgatgaagt atcggcgatg acagaatttt ataaaggtga aacaccaaat gatattccta
    11881 tgatgccaga tgaaattaaa tatgaagatt acagagaatc attaaactta ccagatatag
    11941 ttgcaaatgg tgctttacca attggattag attatgaagg tgttacacta caaaaaatta
    12001 aattaactga accagcaatg atttcatcag aaaatccgag agaaattgcg catattgctg
    12061 aaattatgat gaaagaaatt gacatattaa atgaaaaata tgcgatttgt atcgcagact
    12121 caagtggaga gtttaaagct tataggcatc aagtggctaa ctttgccgaa gaaagagaag
    12181 acattaaagc gattcatcaa ctaatgattg aagacttaaa gcaaagagaa atggacggcc
    12241 catttgaaaa agattcactt tatattatca atgattttaa aacatttatt gattgcacgt
    12301 atattccgga agatgatgtt aaaaagctta ttacaaaagg accagaactt ggcttgaaca
    12361 ttttatttgt cggcattcat aaagaattaa tagatgctta tgataaacag attgatgttg
    12421 cacgtaaaat gattaaccaa tttagtatag gtattcgtat ttcagaccaa caattcttta
    12481 aatttagatt tattcaacga gaacctgtta ttaaagaaaa tgaagcatat atggtcgcaa
    12541 accaagctta tcaaaagatt agatggttta aatagcaatg aattaaatag gagggaggta
    12601 tgttatgaat tttaatgata ttgaaacaat ggttaagtcg aaatttaaag atattaaaaa
    12661 gcatgctgaa gagattgcgc atgaaattga agttcgttct ggatatttaa gaaaagctga
    12721 acaatataag cgattagaat ttaatttgag ttttgcacta gatgatattg aaagcacagc
    12781 aaaggacgta caaactgcaa aatctagtgc taataaggac agtgtaactg ttaagggaaa
    12841 ggcgcccaat acgttatata ttgaaaaaag aaatttgatg aaacaaaagc ttgaaatgtt
    12901 gggtgaagat atcgataaaa ataaagaatc cctccaaaaa gctaaggaaa ttgctggcga
    12961 aaaggcaagt gaatatttta ataaagcaat gaattaatat tgaggtgaag atatgggtgg
    13021 atataaaggt attaaagcag atggtggcaa ggttgatcaa gcgaaacaat tagcggcaaa
    13081 aacagctaaa gatattgaag catgtcaaaa gcaaacgcaa cagctcgctg agtatatcga
    13141 aggtagtgat tgggaaggac agttcgccaa taaggtgaaa gatgtgttac tcattatggc
    13201 aaagtttcaa gaagaattag tacaaccgat ggctgaccat caaaaagcaa ttgataactt
    13261 aagtcaaaat ctagcgaaat acgatacatt atcaattaag caagggcttg atagggtgaa
    13321 cccatgatga aagatgttaa gcgaatagat tatttttctt acgaagaatt aacaatttta
    13381 ggtggtagta aattgcctct cgtaaatttt gaattgtttg atccatcaaa ttttgaagaa
    13441 gctaaagctg ctttaattga aaaggaatta gtaacagaga atgacaagtt aactgatgca
    13501 ggttttaaag tggctacatt agtcagagag tatattagcg ccattgtaaa tattcgaatt
    13561 aatgatatgt attttgcacc atttagctat gaaaaagatg aatatatttt gttaagccgg
    13621 tttaaaaata atgggtttca aatacgaatt atcaataaag acattgcatg gtggtcgatt
    13681 gtacaatcat atcctttatt gatgagacaa gaaaagtcca atgattggga ctttaaacaa
    13741 attgacgatg aaacattgga gaacttaaat aatgaaagta tcgatacgat tgggcgtgtt
    13801 ttagaaattg aaatatacaa tcatcaaggt gaccctcaac aaagtttata taacatttat
    13861 gaacaaaatg atttgttatt cattcgatac ccattaaaag ataaagtgct gaatgttcat
    13921 attggtgtca ttaatacatt tatacgagaa ttatttggat tcgatactga tgaaaatcat
    13981 attaataaag cagaggagta atgacgttga gtggaaaaat tagtgttaaa gctgaaacga
    14041 ttgcacatgt tgtaaaagaa ttggaaagca taagtcaaaa gtatgatgaa atagctcaaa
    14101 actttggaaa aatagcgcaa ttaaattact acagtagtga aaaagctgca cattctatgg
    14161 aaaatggcta tagtagtgct gcaacagtca ttagtggtct caaaggtcca ttgagtacac
    14221 tcggtggtgg cgtcatgaat tcagcacaaa agttctttga agcagatgaa cattggggta
    14281 cggaatttgc caagctttac tataatattg agggataggt gcatgacatg acaaaagata
    14341 ttgaatatct aacagctgat tatgacaatg aaaagtcatc tatccaaagt gtaatagatg
    14401 caatagaggg tcaagacttc ttagatgtag atacaacaat ggatgatgcg gtaagcgatg
    14461 tcagttcttt agacgaagat ggcgcaatat cattaacaag tagtgtagta ggtccacaag
    14521 gatctaaatt aatggggtat tatcaaaatg agttatatga ttatgcatct caattagatt
    14581 cgaaaatgaa agaaattatt gacacgccat ttatagaaga tatagataaa gcattcaaag
    14641 gtataacgaa tgttaaattg gaaaatatac taattaaaaa tggtggtgga catggtagag
    14701 atacctatgg ggcttctggg aaaattgcaa agggagatgc caagaaaagt gacagcgatg
    14761 tttatagcat cgatgaaata ttaaaatcgg atcaagaatt tgtaaaagta attgatcagc
    14821 attacaaaga aatgaaaaaa gaagataaga aattatctaa aagtgatttt gaaaaaatga
    14881 tgactcaggg cgcttcttgt gattacatga cagtagctga agcggaagag ctagaggagc
    14941 aaaagaaaaa agaagaagct atagagattg cagcactagc tggtatggta gttttatctt
    15001 gtattaatcc tgttgctgga gcagtagcta ttggtgctta ttccgcttat tcagcagcaa
    15061 atgcagccac aggaaaaaat attgtaactg gaagaaagct atctaaagaa gaacgaatca
    15121 tggaaggact ttcgcttatt ccattgccag gtatgggctt cctcaaaggt gctgggaaaa
    15181 gtttaatgaa attaggcttc aaaggcggag aaaaatttgc agttaaaaca ggattgcaaa
    15241 agacaatgca acaagcagtt agtcgtattt cacctaaaat gggaatgatg aaaaacagtg
    15301 tgttgaatca atctcgtaac tttgctcaaa atactcatgt tggacaaatg ctgagtaaca
    15361 tgcgtggtca agcaactcat actgttcaac aaagtagaaa ttggattgga caacaagcac
    15421 aaaatgtcaa acgaatagtg aataatggac ttgataaaga aatagcacat ccatttaaac
    15481 aacaacttgc accagcggga atgggtggta taaaatttgc tgaaacaact actttgagaa
    15541 acatgggtca aaacataaag cgtgctgtta caccacaaaa tcacgtgaca catggtccaa
    15601 aagatagtat ggtgagaagt gaaggcaaac atagtataag tagccatgaa atgaattcat
    15661 caaagtatgt tgaatcacca aactacacca aggttgaatt cggagaacac tatgcaagac
    15721 ttagacctaa gaaactaaag gcgaatattg aatacacaac acctactggt cacatatatc
    15781 gaaccgatca taaaggtcgc ataaaagaag tttatgtaga caatctctct ctaaaagatg
    15841 gggatcgtaa tagccatgca caaagaactg tggggggaga ggatagatta ccagacgatg
    15901 atggaggtca cttaatcgct agaatgtttg gtggttcaaa agacattgat aaccttgtgg
    15961 cacaaagtaa atttatcaac cgtccattta aggagaaagg tcattggtat aatcttgaga
    16021 aagagtggca agagttctta aactctggga aagaggtgaa aaatattaaa atggaagtaa
    16081 aatatagcgg taatagtcaa agaccgacta tatttaaagt tgaatatgaa attaatggtg
    16141 aaagaaatat tagaagaata ttaaataagt agaggtgcca acatgacatt tgaagagaag
    16201 cttagcaaaa tatacaatga aattgcgaat gagattagca gtatgatacc ggtagagtgg
    16261 gaaaaagtat a
//
LOCUS       DALAAR010000055        16031 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000055 DALAAR010000000
VERSION     DALAAR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.057"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(123..392)
                     /gene="rpsN"
                     /locus_tag="O2458_001914"
     CDS             complement(123..392)
                     /gene="rpsN"
                     /locus_tag="O2458_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCZ3887776.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            complement(846..995)
                     /gene="rpmG"
                     /locus_tag="O2458_001915"
     CDS             complement(846..995)
                     /gene="rpmG"
                     /locus_tag="O2458_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ3887777.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(1086..2603)
                     /locus_tag="O2458_001916"
     CDS             complement(1086..2603)
                     /locus_tag="O2458_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCZ3887778.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2807..4261
                     /locus_tag="O2458_001917"
     CDS             2807..4261
                     /locus_tag="O2458_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ3887779.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            4400..4504
                     /locus_tag="O2458_001918"
     CDS             4400..4504
                     /locus_tag="O2458_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887780.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            4479..4793
                     /locus_tag="O2458_001919"
     CDS             4479..4793
                     /locus_tag="O2458_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887781.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(5086..5889)
                     /locus_tag="O2458_001920"
     CDS             complement(5086..5889)
                     /locus_tag="O2458_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ3887782.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(5947..6861)
                     /locus_tag="O2458_001921"
     CDS             complement(5947..6861)
                     /locus_tag="O2458_001921"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCZ3887783.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(6863..7924)
                     /locus_tag="O2458_001922"
     CDS             complement(6863..7924)
                     /locus_tag="O2458_001922"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCZ3887784.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(7930..9210)
                     /locus_tag="O2458_001923"
     CDS             complement(7930..9210)
                     /locus_tag="O2458_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCZ3887785.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            9401..10783
                     /locus_tag="O2458_001924"
     CDS             9401..10783
                     /locus_tag="O2458_001924"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ3887786.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(10837..11412)
                     /locus_tag="O2458_001925"
     CDS             complement(10837..11412)
                     /locus_tag="O2458_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887787.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            11743..12597
                     /locus_tag="O2458_001926"
     CDS             11743..12597
                     /locus_tag="O2458_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887788.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(12740..13273)
                     /locus_tag="O2458_001927"
     CDS             complement(12740..13273)
                     /locus_tag="O2458_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ3887789.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            13412..13600
                     /locus_tag="O2458_001928"
     CDS             13412..13600
                     /locus_tag="O2458_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCZ3887790.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(13713..14315)
                     /locus_tag="O2458_001929"
     CDS             complement(13713..14315)
                     /locus_tag="O2458_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3887791.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(14312..15403)
                     /locus_tag="O2458_001930"
     CDS             complement(14312..15403)
                     /locus_tag="O2458_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ3887792.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            complement(15407..>16031)
                     /locus_tag="O2458_001931"
     CDS             complement(15407..>16031)
                     /locus_tag="O2458_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887793.1"
                     /translation="ILELPEDVKPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNE
                     WRQRLMVTPFTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFL
                     WLGASLLITFGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVL
                     PSYHLRKLGVDIASNHHINLISFAIILLYALGSIIAVYCISHFKRAE"
BASE COUNT         5260 a         2791 c         2209 g         5771 t
ORIGIN      
        1 aatgcaaatt aaaaaccgaa tgtttcctta ttaatatgag aaaacattca gtttatagca
       61 ttcgtatata ctgtacattg ttttattgat attgtaaatg ggctaaagta cgaaatttga
      121 ttttaccaac ttgatttttt aacacctgga atttgtcctt tgtgcgcatg ttctctaaac
      181 gcaatacgag acatttcaaa tttacgtaat acacctctag gtcttccagt tactttacat
      241 cttctagtta aacgtgtagg tgatgaatct cttggtaatt ttcttaacgc ttcgtaatca
      301 ccttttgctt ttaactcttt acgtaattcg taatatttat ttactaactc ttctcttttt
      361 ctctcttttg ctattttaga tttcttagcc atatatagtt acctcacttt ttatcaaatc
      421 gtaattatta cgatttatat aataacacaa tcctcacata attcaacagt aaagtttcat
      481 ttctttgtta aatcattttc taaaccatca tatttaagat taaagtgtat tatcgaacat
      541 ttataaaaca tacccagaat tacttcgtac ttattaaatt ccgctttgaa attttaaatt
      601 ctactatatt aaatactctt tcaagcattc gctaacaaag tgactttata aaatcagtaa
      661 tctatttcct tctataaata ttctcaaatt ttaataaagt aacaactaat ataatcatat
      721 attgttctac cgatgaaact caatccatta atcactcttc acaaaaattt cataaaaaag
      781 cgagctcccc aatataatag gtaagcccgc tttattttca aatcgtcgta tttgaatgat
      841 aagaattact tagtttcacg atgtaacgta tatttgttta atcttgggca atattttttc
      901 atttcaatac gctcaggatt attacgttta tttttagtag tgatatagtt acgatcgcca
      961 cattctgtgc atgctaatgt tacgtttacg cgcacgttaa acaccctctc ttgttaggaa
     1021 tctttacgat ttattatgat atcataaact gctcaactac gcaatataga aactacatat
     1081 caaatttatt tttcaaagtt ttcgtatgtt tcatcatttt cagtttcaag atcaatagaa
     1141 tttatatcaa tacctaatgc ttttgcaaca cctttaccat attctgggtc agctttgtaa
     1201 caatgacgaa tatgacgtcg tttaacatca tccgttacgc cttccattgc atttgctgta
     1261 tttgtaaaaa ttctttcttt agcgtcctct gattgtaatc taaacaattt acctggttgt
     1321 tcaaaataat tatcatcatc ttgacgttga ttatattcat agccgtatcc atcagttggg
     1381 aatggtggct ttttatattc aggttgagaa tcaaatttac catggttatt tggataataa
     1441 tgtgttcctc caccttggtt attgtcaact acgcgcattt gaccatctct actaaaagga
     1501 caaatatttt caataccaac acctttaggt tggtttacag gaatctgcca atgattaact
     1561 cctaatcgat atctttgcgc atcgccatat gagaataaac gcccttgcag cattttgtct
     1621 ggagaaaaat ctaatcctgg aataatatta gttggtgcaa acgcagcttg ttcaacatcc
     1681 ataaagtaat tatctggatt tctatttaat tcaaactctc caacttcaat tagaggatac
     1741 tcatcgtgat accatacttt tgttaaatca aatggattat ctttatggtt tttagcttgt
     1801 tcctcagtca ttacttgaat atacattgtc cattttggat aatcaccttt ttcaatggct
     1861 tcgaataaat cgcgttgaga tgaatcacga tctgtagcta taatttcagc agcttcttca
     1921 tcagttaagt tttcaatacc ttgttgcgtt ctaaaatgga atttaaccca aacacgttca
     1981 ccagaatcat tatacataga gtatgtgtga gaaccgaacc catgcatatg acgtaaatct
     2041 ttaggaatcc ctctatctga cattaagatc gttacttggt gcaatgcttc tggaagaccc
     2101 gtccagaaat cccagttatt ttgtgcatct ctcatatttg ttctaggatc tcgtttcacc
     2161 gcacgattta aactaacaaa taactttgga tctctaaaga agaatactgg tgtgttattc
     2221 cctactaaat cccagttccc ttcttcagtg tagaacttta acgcaaatcc tcgaatgtca
     2281 cgctccgcat cagcagcacc acgttctcct gctacagtag agaaacgggc aaacatttcg
     2341 gtttgcttac ctatttcaga gaatatttta gcattcgtat attttgttat atctttagtt
     2401 acagtaaatg tcccaaatgc accagaacct ttggcatgca ttcgacgttc tggtattact
     2461 tctctatcaa attgagacat ttgctctaaa aagtaaatat cttgcattaa aagaggtccc
     2521 ctaggccctg ctgtcatact attttctcgg tctgatactg gatgcccaaa aacaccagtt
     2581 aactttttgt cttgttgtga catagtcatc cctccacaat ttataataat tataatttaa
     2641 tacatattat aatttaaccc catcaaaatt acaattaaac atgctcaaga tgattaaaaa
     2701 atcagaaaat tttaattttt tcgctttaca gtactaaata gttattgaat agtacactta
     2761 aaaagcttac aataatgggt atatttaaat agatgggaga ctaaatatgg aagataataa
     2821 aatgaaccgt agtcttaact caagacacat ttccatgatt gctataggtg gtgcaattgg
     2881 gactggtcta tttgtagcta ctggtaatat catttctcaa gctggtcctg gaggcgctat
     2941 actcgcttat cttgttattg gtgtcatgct atatttctta atgtcatcaa ttggagagtt
     3001 ggcaacattt tatccagtat caggttcatt cagctcttat tcaacacgct ttattgactc
     3061 atctcttggc tttaccatgg gatggttgta ttgggcattg tggtcattag ttacaagtgt
     3121 tgatgtcata gtagcgtcaa atgtgcttta tttctgggac acatttaaat ttttccaccc
     3181 cattacttgg agcttaatct ttattacaat tttactatta ttaaacattt tttctgtaaa
     3241 atcatttgga gaaactgagt tttggttatc attgattaaa gtgttaacaa ttatcgtatt
     3301 cgttattttt ggctttttaa tgattttcgg tatcttaggt ggtcatacat atggatttga
     3361 aaactataca aaaggccaag caccgtttgt tggtggtatc tctggtttct taggcgtatt
     3421 attagtcgcc ggattttcgg ttggtggtac agaagtagta gcagtaactg ctggtgaatc
     3481 agatgaccct aaaaagtcta tgcctaaggc aattaaacaa gtattttggc gtattctttt
     3541 attctatgtc ttatcaattg cagtaattgg tgcaattatt ccgtacacag atccatcatt
     3601 attaagagca agtagttcaa taagtcaaag cccatttaca attgtattcg atagagtagg
     3661 catagccttt gcagcatcag taatcaacgc ggttatttta acttcattat tatccgctgc
     3721 aaattcaggt gtttatacaa caggcagaat gttgtattcc ttaagttcag acaaaaaagc
     3781 accccaattt ttaagtaaat taaacaagac aactaagtta cctttaagag cattattaac
     3841 tacttatgca gtcgttgtta ttgttattat ttatgcaaac tttaattcaa atgccgtttt
     3901 taatttactt gaaattattg gttcaatgat tatagttgtt tggggatcaa gcatttggtc
     3961 acaaatacga ttgcgacaag ctattaaaaa acaaggtcaa gaccctaata aggtcctacc
     4021 atataaagca cctttttatc cattaggacc aatcattgtc atcactacac tattattctt
     4081 gctatttggt ggctcagttg aatatatttt aaaagatcaa tggttaaatg cttttaaaaa
     4141 ctttttacct ttaatcattc tagcgttgat ttactttatt cataaaatca ttcacaaaac
     4201 aaaatttgta aagctagaaa caattaattt aaaaccacac gattatgaca atcaaaaata
     4261 aataaattct catacggcag atgctttaga ttatctatag catttgccgc tttttaatgg
     4321 tgtaaagcag atatacttta atatcttttt ctagtataaa gtgtattaac tagtattctg
     4381 tttatatttt attattacca tgttaccatt ctttatttta gatattttgc atatcgtgta
     4441 taatatagat aacaataact tttcaggggt gaaattcaat gattggacaa acaaatttat
     4501 ttgatgatgt actaaagcaa gatgaaagat ttgttattgt agttcaagca ttagaagaaa
     4561 agaacggaca attattaaag agaactttaa gggaatatcc cggtttaaac cataaacaaa
     4621 tgaatgattt atttatgcac ttaaaggaat tattttccga agaatcattt gctgaaaacc
     4681 aatcagcgtt cagtattaca gtttatacaa atttagatta tactgctgac caaatatatg
     4741 ctcatgtaaa acgtttcaga ggtaagcatg actggacaca aacagctaaa taaaatcaag
     4801 atgcttgcag tgattttaac aattaatgtt agattgtcac tgcatttttt aatgcattcg
     4861 ttattattca gaatgatcaa atttatttat ctaccttccg tgactagtta atacttgatt
     4921 tattcaaaaa atatacattt actctattcg tttgatatca ttactaatct aaaatatgta
     4981 tttcaaatcc tccatagtat aactgaagtc attttcacaa aaataaaaag ccttaaccac
     5041 aagatatctt attcaaatat catgtgttaa ggcttatttt aaaatttata acaatttttt
     5101 gagcgcatgt ggaataccat tttcattatg atccaacgta acttcatttg catattgctt
     5161 aagttcatct gatgcattcc ccatagcaat agctagacct gcgacttcaa acattgattt
     5221 atcattcata ctatcaccaa aagcaatgac ttgatccacc gaatatccca atttgtgaca
     5281 taacgcttta attgcattac cttttgaaac gtctttggcc ataaattcta agaagaatgg
     5341 cttacttgtc gtagcatcta cattatcatt gaacacacca ttcaaatcaa ttctagcttc
     5401 tgtaatattc gctacataat ctacacccat gaccttgggt acatcgcctt gaatatacgc
     5461 tttgatatca tcaacacgtt tcatcggtaa acctgttaat tctgcttcaa tattcatata
     5521 ctcatgttcg ctgtcgtaaa taatttgacc atcatgatat gtaagaacga aaaagcctct
     5581 atctcgacaa taatctacaa tttcatcgaa atcttgctta ccaatcgatt tacttacttc
     5641 tacttcttca ttagtcatgt taatcgtttt actaccgtta taactaataa tatagctatg
     5701 atgttcaggt aattttaaat ctctagcagt tggaatcata ccttcagtag gtctaccaga
     5761 tgctagaaca acataataac cttcatcttg aattgctgtt aaataatttg cagtttcttc
     5821 tgatatcaca ttatctgaat ttagcaatgt gtcatccata tctaaaacga caactttata
     5881 atttgtcata tcgcattaaa tctcctctct attctgcatt taatactatt ttacaatata
     5941 aagcgtctat tggtatacaa ttcgttcttt aacaccactg atattaatat cgaccaattc
     6001 agaatggcat gatactacct gactatttaa agagcgaacc aattccccac tattttcttt
     6061 acgactaaat attaaaatag ttggtccagc accactaatt acagttgcat aggcattatg
     6121 ttgactagca attgttttca cttcatcaaa ttcagcaatt aaatgctgac gatacggttc
     6181 atgaaagcca tcttgttgca tgagtttacc tgctaattca taattgtgct gtgctaatgc
     6241 acaaatcatt gtattactaa ttgcactact tttaaccgct tcactatgtg ttaatttttg
     6301 tggtaaagca cgtcttgatg cttctgtttt tagttcataa gttggtatcg ttacaatcac
     6361 atccacgtct ggtatgtcga tatgtgcaac tgacgtttct ttcgagacat cattataata
     6421 tccagcgatt aaaccaccat aaatggtcgg cgcaacatta tcaggatgtc cttcgatttc
     6481 agtcgctaat tgtaatacct catgtttaga cagttggata tcaccaaaat aatttgcgat
     6541 atatatagct cctactaaag cagaagctga cgaacctaac cctcttgcca atggaatatc
     6601 acttctcatt tcgatacata aattaggcaa gtcaacacta tatttagaag caacttgttg
     6661 tgctacatga taaataaagt ttgtttcgtc agtaggcaat tgcttagatg catcatcatg
     6721 gaaaatatat tcccattttg tccctgatgt ttcctttaca gacagatgca aaaatttatc
     6781 taaagccata cctatagaat caaagccaac tccaaggttg gctgttgatg caggaattgt
     6841 taactccaaa acattcgaca tgttataaag ctcctttaat ataatcgata atgctatctt
     6901 tatcatttgg caatggcttt atcggattat ctagtagtga aatagcagta tcaggatctt
     6961 ttaatccatt accagttaaa atagcaacta cttttttacc ttgaggtaat ttaccttgtc
     7021 tatgcaattt aattaaacct gcaatagaag cattactcgc tggttcacta aagacacctt
     7081 ctttagttgt cattaactga tatgcttcta gaatttcatc atcagtaaca ctatctatta
     7141 atccatttga ttctttaaga gcattagtcg ccttatccca actagcagga ttaccaattc
     7201 gaatagcagt tgcaatcgtt tcaggatttt taatgacttt attttgaaca attggtgatg
     7261 cgccttcagc ttggaagcca aacattttcg gcaattgtga tcctttagct tcatgatatt
     7321 ctttaaagcc tttccaatat gcagtaatat tacctgcatt acctacagga atcgctaaaa
     7381 tatcaggtgc ttcaccgtct aattgttgta caatttcaaa tgagcctgtc ttttgtcctt
     7441 cgattctaaa tggattgaca gagtttacaa gctcgatttc gccacttttt gcaatttctt
     7501 ttacaatttc taaagcttca tcaaagtttc cttcaataga aacgatttct gcaccataca
     7561 ttactgcttg cgacaattta cctaatgcaa ttttaccttc tggtattacg acgatagctt
     7621 ttaaacctgc tctcgctgca tatgctgctg cagacgctga tgtatttcca gtcgaagcgc
     7681 atattacaat tttcttacct tgctcttttg cttttgtcac agccattacc ataccgcgat
     7741 ctttaaatga acctgtcgga ttggcacctt catacttcac atataaatct atgcctagta
     7801 ttttagacat attttcacaa tgaatgagtg gtgtatttcc ctcgttcaat gttaattttg
     7861 gtgtattttc atttactggt aaatgtgctt taaactcttc tactaatcct tgccatcttc
     7921 tcattataat taaactcctt ctactgggta tactttgtca acttcgtatc catgcttagt
     7981 gattaattct tccggtgatg attccaatcc aactataaca gcggcatatg attgattatc
     8041 gtaatttgca actcttaatg atcggtgaaa tggtaatatt gcctttaact cattttcaaa
     8101 attttcaatt gaacctttga catgattcac tactacaaag aaattggatt tttctttaat
     8161 attaatttct gcatctgaat ccatcatttc ccgtgtttta tctgtcttta attcaaaatg
     8221 tggtggcaat gtgtgtaaat ctgattcaaa gaataatgct acattcaata aatcactgac
     8281 aacggcacta cctgttgcta aactgcctgc tccttttcca taaaacatcg tgtcaccaac
     8341 ggcatcacca ataacataaa tcgcgttata ttcatcctct acagctgcta attgatgctt
     8401 tttatcaatt aacgttggtt ctaccgatgc attaactttg ccattttcat atattccctt
     8461 accaatcaat ttaattttat accccagttg atcggctaca ttaatatcag ttaaagttac
     8521 gccactaatt cctcttcgtt taacgtcgtt taatttaatg acttggttaa atgataaata
     8581 tgatgtaatg acaactttac gcgctgcatc taccccttct acatcatcag ttggatccgc
     8641 ttcagcaaaa ccaagtcttt tcgcttcatc aagtgcttcc tcaaatgtcg tttgctcttt
     8701 agtcatttta gataaaataa aattagaggt accatttaaa attcccataa attttgaaat
     8761 attattcgca ttcaaaccat tatttatggc atttacgatc ggaataccac ctgctacact
     8821 cgcttcaaac tttaaagcta caccattttc ttctgctaaa tcttctaaaa gtttaagatg
     8881 tactgctaat aaatctttat ttgcggtaat aacatgtttt ttatttttaa gtgctgttct
     8941 taaccaatct acagttggtt caattcctcc catgacttca acgataatat ctaatgaatc
     9001 atcatttaaa atttcattaa catcttcagt taaatgatat tggctaatat ttagcggtct
     9061 ctttttagat ttatctcgaa caagaatatg ctttatgaca atatctttat ttaatgtatc
     9121 ttgaatttgc tgtcggttct cttcgatgat tttaacaaca ccagatccga cagtacctaa
     9181 tcctaataat gctatattta attttttcat tttatgtacc tccgtttgtg cttttgagaa
     9241 aaacaaatgt attattgaaa aacactagat gatagtatag tatagaatta ttctttattt
     9301 ttgtaaagtt cgtaatattc aaaatataaa tacataatat cattcttact tacaaaactg
     9361 aatacaattt tctgattttt tagaaaggtt ggcatgcact atgaaagttt ccaaattcgg
     9421 tggtagctcc gtctccaatg ctactcaaat taaaaaggtt ttaaatattg ttaattctga
     9481 tccagagcga aagattgtta tcgtttctgc tccaggtaaa agacatgata atgatattaa
     9541 aacaactgat ttgttaatca gattatatga aaaggtcatt aatcatcttg attatcatga
     9601 taaaaaaaga gaaattattc agcgttatga tgatattgta aaagaattgc aaatggatga
     9661 aagtatttta cggacgatag atgtgacttt ggaacattat attaatcaat taaaaaatga
     9721 accaaagaga ctattagatg cattactttc ttgtggtgaa gattttaatg cgcaattgat
     9781 agctttatat aataatagtc aaggtatacc aacaaaatat atatcaccga aggaagcggg
     9841 tatttttgtt actgacgtcc cacaagaagc acaaatatta gatagtgctt actctgaaat
     9901 taaaaaatta agagattatg atgaaaaact aattatccca ggattttttg gcgtatctca
     9961 tgaaggatat atagttacat ttccacgtgg cggatcagat ataactggcg ccatcatatc
    10021 aagtggtgtt agagccacaa tttatgaaaa tttcaccgat gtgtctggta tttataaagc
    10081 taatccaaat atcatcaaag atcctgaatt aatcgaagaa atcacttatc gagaaatgcg
    10141 tgaattatct tatgctggtt ttagtgtttt ccatgatgaa gccttacaac cactttataa
    10201 agatcgcatc cctgtggtga ttaaaaatac gaaccgccct caagataagg gtacatttat
    10261 agtgcatgat agagaaataa atgcgaaaaa tgtgattagc ggtataagtt gtgataaaga
    10321 ttttaccgtt attaatatca aaaagtattt aatgaataga caagtcggct ttactagaaa
    10381 gatattaggt gtcttagagg ataataatat ttcatttgat catatgcctt caggtattga
    10441 tactatcagt atcgttatgc gttcaaaaca aattcaaaat aaagaaacaa aagtattaaa
    10501 tgaaattcgt caaaaatgtg atgttgatga gctaagtatt gagcatgatt tagcaatatt
    10561 aatgattgtc ggcgaaggta tgaataaagt catcggtact gcaaataaaa ttacacatgc
    10621 cttagctgaa tcaaaaatta acttaaaaat gattaaccaa ggtgcttcgg aaatctcgat
    10681 gatgtttgga attcatgtag atgatgctga aaaagctgta ttatctacat atgagttttg
    10741 ctatcacggg gttgcattaa aaaacttgtg tgataaaaac taatataaaa gcaattcgta
    10801 acacatgtat tcaactgcgt tatgaattgc tttttcttaa ttatttattg ttctctggat
    10861 tgcatcctta atacgttgct cacctactac aaattttaaa ataaaaaatt gatagtagct
    10921 taatgcatct atgactcgtt tataattgga ataatgatga tactgatggt gttgcattag
    10981 atcatacatt agaacgatct ccggtttaat ataatccaca tcccaattta aactatggaa
    11041 gtatatgtta ctcttaggta tacgtatatt cggatccaat ctaaaaatcc attcatcgtt
    11101 tactacatca taaacgaaaa tactcattat tgttgtatca tcatattgca cataaacttt
    11161 tgcaattttt gaaagtgata aatcttcttt agctattgct tgatcttgct tatctatgta
    11221 tagtaacgca tatgattgcg gaataacttt cacaatatca cttaaacgct tacgttccac
    11281 gcatatttca acaatagaag gtaattttaa ttcatcattt aagaaaagct gcatggcaac
    11341 ttcaccgtga aatttaaata attgcgatga tgttttcatt aaattgtcca ctaattgttc
    11401 tttagcattc atttcattca ctctcaaata cacctcctaa gcaagcgctt tcacaaatat
    11461 cattatacat tttaaaatta atgaattcaa tcttcaaaat actatgagtg acctaattct
    11521 gtcatttaaa tgaccgttat aacataaatt cgacataaac ttgaattcac gaatctttca
    11581 cgtcatattc attgaacatc aatatctgtc agtttataat ttgattataa caatgatgaa
    11641 ctaaaatcaa ctaataaaaa tgtgggaaat tgaataacaa ttaatcttta ttaattatta
    11701 ttgtatacaa agttaaagaa gtcaaaaagg attggtgaaa aaatgtcttt ttatgttgta
    11761 cttatcatta tcattgtagc attaattggt atattagttt taaatcaaag gtatagtaat
    11821 agtaaaatag ataccgaagt ttatgcaaga aagcaactta ttaaaaagaa caaagcatta
    11881 agtgcagaaa atgctgaatt aagaagtcaa atgctgagtt ctaataatga tgttggccac
    11941 catgcataca aaaatgctaa aagagaatta cgcaagattt tagatagcta tctcgaaaat
    12001 ggtaaattaa aatattacga tattatcgtt acaagcaatt tagctactaa acatccgttt
    12061 ttcgagtatg cacgttcatt tgattttatc attgtttcag atatcggttt aataaacgtt
    12121 gatgtaaaaa gctggggtga aaaaacattt tatcactttg atgtacctga tgagcatgat
    12181 acagaaataa gcaatagtaa tattgaaaaa gttgtcggtc attacattag ccagcaatat
    12241 catgatcagt ttaattcatc aagaaagtct atctatactt ttacagaaac agttcaacca
    12301 aatcgtgtta tttatgattt ttatgattat gatccgtatc aattagcagc aaataatgcg
    12361 aaagcattaa aggatcatat tgaacaaaac tttaacttta aagtccaaag taccggtgtt
    12421 atttacttta gtgatggtac agttaatatc attcaaggtt ctgaagaaag agataaatat
    12481 gttgatacag tttcaacaaa atcttcgtta cgacgtatta ttagcgaagc catagagcta
    12541 tctaaacacc ctttaaataa agaacaggtt gatcaaatca cagctatatt caagtaaaat
    12601 caaaggcata ataatccatt acgctcgata cgcactatgg ttattatgcc tttaaattat
    12661 ttctatatat aaatttatgg taataataaa tcgatgcttc aagtatattt tactttaata
    12721 tatataatca cctatttaat tatttactcc aaatatttaa tttctgttgt ttagctttat
    12781 tttgtgcttc tataaataca tttctatatt tgccatttgg tgaaaaatac ttctctctag
    12841 caagtccctt ttccactaat tccttattgt acatacgatc tttacttatc catacatacg
    12901 ccaatgttct accatagcga tcttgttttt ctttatcata ttctaaataa acatcttgat
    12961 ttgttaatgt cttcttacta taatttgatg cttctttgcc aaatggttgt acaggcgtat
    13021 tcggtttcac cgtttctggc gtatcaaccc ctataagcct aactttaatt tctttaccat
    13081 tttgatttgc aataaatgta tcaccatcca caactctttg aacatgtact ttatctttac
    13141 catttaaatc ttgtacagtt tcatgattcg tccccttttt gaaaggaccc gtatgattca
    13201 taaattgaaa tgctaataca cctacaataa tgatggctac cacaatcata gcaagcgatt
    13261 tatttgactt catattgcca cctacttctc ttttttcatt ccgattaatc atataaaagt
    13321 taagaaatat cgtcaatagc catatctaca tatttcaata ttttggaaag ctatagcatg
    13381 atataatgtt attgttaagg agtgaaaata aatgaacatt tcaaatgaaa atacagtcac
    13441 attaattgca gtaattatcg caattatcat tggtattttc ctacaaatat ttttcaaatt
    13501 accattaata gtgacggcag tactatctat tttattgggt atctttgtag gctttatcgt
    13561 ttacttgatt gtttcgtttt ataataagcg aaaaaattag ttaacaaatc taaattgcga
    13621 gtaccaaaat aaaagtattg agtaagattt tgattattga ctcactttcc aagtctctta
    13681 aatcaaaaca ctcaatactt ttttgtttgt atttagatcc agcctttttc atttgccttt
    13741 ttccaagcat caaaacgatt atctgcaaat aatttatcaa ttataacaga tgtataattt
    13801 ctaactgttc catctgtcaa aaataatttt tcacttattt ctttactact taaaccattg
    13861 ccaatttccc ttaatacaat ttgttctttg ggcgttaatg ggtttttatc tacaaaaaat
    13921 gaagtcatca atgtggcgct atattctttc tctccgttat ttactttatt aatggtttcc
    13981 accaattctt ctatagaacg ttcttttaaa acatatgcat ccacatcatt cacaactgct
    14041 ttttcaaagt atcccggtct tttaaaagtt gttacaataa tcactttaat attcaaatgc
    14101 ttttttctaa tttccgctaa aacttcaagt ccagtcatgc ctggcatttc tatatctaaa
    14161 aaaacaacgt taggattata ttcttcaata agtttcattg catcgagacc attatcagta
    14221 tctgctaaaa tttcaaaatc accatgtagt ttaattaatt gaaccattgc ctgtcgtaac
    14281 atattttgat cttctgcaat aattaaagat gtcatgctat tcctcctgtt gagatttcaa
    14341 tgattatttt agttccattt gttgagtcga ctgctaaagt tccatttaaa tgttgtacac
    14401 gctgtgaaat acttttcacc tcacaatcac tatcgatacc tttgccatca tcttcaatca
    14461 taagcagtaa tttatgattg tttacagttt ttaatttacc atgaactttt gaagcatttg
    14521 catgtttaat aacattattt atcgcttcac gcgtaatcat aactaacata gattgtttag
    14581 taggacttaa tacttgcgct aattctttat tttcaaatgt aaaatcaata tcagcatctt
    14641 ttaaaacttt acgtatacta tcaatctctt caataaatga tggtaatttt acatcatcaa
    14701 taatttctcg aactttgttc aatgattcac gagataattt attaattgct aataattcag
    14761 cttttacttt ttctacatca gcatctatta gtttataagc taattctgat tttaatgata
    14821 aacttgcaaa cacatgccct aacgtatcat gtaagtcttg accaattcta tgtcgctctt
    14881 gttcagcaat taatgtatta atatgtctat ttttttctgc tatttctttt tgatattcac
    14941 ggtttttcat ttttttaaaa ttatctagca ttattaacga tataacgaca taataaacca
    15001 tcattgcaac aaaatagttg ttatacaata agtacgttat tattgtacat ataatcattg
    15061 ttagtatgaa aagattagtt gccgtttttt taacattatt tttaaaagta aatggaacgg
    15121 caaaagcact ataaaagaaa aacaaactta gcattggatg tacactgaaa acaaaataac
    15181 aaataataaa ataatgaata atcaacaaaa tgtaaaacat taatgtatta agtctattat
    15241 ttactataac taatataaga tacgacaacg aaaagacaat gactgatata atatacaacc
    15301 atttgggacc atatacttca ttaaaaaata taccggcaat aggaaaaatc agataaacta
    15361 tagacgatat ttcagcaatt gaagtatctt ttaaaaattt catattttat tccgcccttt
    15421 taaaatgact aatacaatat actgctatta tactccctaa agcatacaag agtattatag
    15481 caaatgatat taaattgata tgatgatttg aagcaatatc tacacctagt ttacgcaaat
    15541 gatagctcgg taaaacatga gcaacatgtt gaagccaatt tggaaatgtg tttatcggaa
    15601 accacaatcc acctaatact gctaaaccaa ttgttacgat attagctaaa gcacttgttt
    15661 tttgaatatc atttaacaaa gaaaataata tgccaaaagt tattaataga gacgcaccta
    15721 accataaaaa tattcctgac tctaaccatt gaactgcact cattgcaaca cctttataaa
    15781 aatgtccaac cataaaaata actaatatcg ctattgcaaa ttgcagcata gttttcacta
    15841 cttttgaaat ataataacta gtaaaagtaa atggtgttac cattaatctt tggcgccatt
    15901 cattttgttt ttcattaata atatctaatg gaaaagttag taaactaaaa cttaacaaac
    15961 tataaaccgt catactatac atatactctt tataaaattt aggtttaaca tcttccggca
    16021 attccaatat c
//
LOCUS       DALAAR010000056        15965 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000056 DALAAR010000000
VERSION     DALAAR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.058"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            3..1169
                     /gene="scpA"
                     /locus_tag="O2458_001932"
     CDS             3..1169
                     /gene="scpA"
                     /locus_tag="O2458_001932"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCZ3887794.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1200..1526
                     /locus_tag="O2458_001933"
     CDS             1200..1526
                     /locus_tag="O2458_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCZ3887795.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1818..1991)
                     /locus_tag="O2458_001934"
     CDS             complement(1818..1991)
                     /locus_tag="O2458_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCZ3887796.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1972..2574)
                     /locus_tag="O2458_001935"
     CDS             complement(1972..2574)
                     /locus_tag="O2458_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCZ3887797.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2845..3666)
                     /gene="nadE"
                     /locus_tag="O2458_001936"
     CDS             complement(2845..3666)
                     /gene="nadE"
                     /locus_tag="O2458_001936"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCZ3887798.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3659..5128)
                     /locus_tag="O2458_001937"
     CDS             complement(3659..5128)
                     /locus_tag="O2458_001937"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCZ3887799.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5312..6388
                     /locus_tag="O2458_001938"
     CDS             5312..6388
                     /locus_tag="O2458_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCZ3887800.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6408..7202
                     /locus_tag="O2458_001939"
     CDS             6408..7202
                     /locus_tag="O2458_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCZ3887801.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            7274..7381
                     /locus_tag="O2458_001940"
     CDS             7274..7381
                     /locus_tag="O2458_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887802.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7392..8954)
                     /locus_tag="O2458_001941"
     CDS             complement(7392..8954)
                     /locus_tag="O2458_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCZ3887803.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9159..10256)
                     /locus_tag="O2458_001942"
     CDS             complement(9159..10256)
                     /locus_tag="O2458_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCZ3887804.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10628..11188
                     /locus_tag="O2458_001943"
     CDS             10628..11188
                     /locus_tag="O2458_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ3887805.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11241..12170
                     /locus_tag="O2458_001944"
     CDS             11241..12170
                     /locus_tag="O2458_001944"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCZ3887806.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12365..12538)
                     /locus_tag="O2458_001945"
     CDS             complement(12365..12538)
                     /locus_tag="O2458_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887807.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12590..13969
                     /locus_tag="O2458_001946"
     CDS             12590..13969
                     /locus_tag="O2458_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCZ3887808.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14089..15117)
                     /locus_tag="O2458_001947"
     CDS             complement(14089..15117)
                     /locus_tag="O2458_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCZ3887809.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15387..15788
                     /locus_tag="O2458_001948"
     CDS             15387..15788
                     /locus_tag="O2458_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCZ3887810.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15806..15965)
                     /locus_tag="O2458_001949"
                     /pseudo
     CDS             complement(15806..15965)
                     /locus_tag="O2458_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5397 a         2796 c         2344 g         5428 t
ORIGIN      
        1 atatgaaaag aaactttcca aaattaatag ctttaagttt aatattttct ttaagtgtta
       61 cccctattgc aaacgctgag agcaattcaa atatcaaagc aaaagataag aagcatgtgc
      121 aagttaatgt cgaggacaag agtgttccta ctgacgtacg taatttagcg caaaaagact
      181 atctttctta tgttacttca cttgataaaa tctataataa agagaaagcc tcgtatacac
      241 ttggtgaacc ttttaaaatt tataaattca acaaaaagag tgatggtaat tattattttc
      301 cagttttgaa cactgaaggt aatatcgatt atatagttac aatttcacca aaaatcacta
      361 agtattctag ttcatcttct aaatacacaa tcaatgtttc tccattttta tctaaagtct
      421 taaatcaata taaagatcaa caaattacta ttttgacaaa ctcaaaaggt tactatgtgg
      481 ttactcaaaa tcataaagct aaactagtac taaaaacacc acgtctagaa gataaaaagt
      541 taaaaaagac tgagagtata cctactggca ataatgtaac gcaactaaag caaaaagctt
      601 ctgtcactat gccaactagt caatttaaaa gtaataatta tacttacaac gagcaatata
      661 taaataaact tgagaatttc aagataagag aaactcaagg taacaatggt tggtgtgcag
      721 gctatacgat gtctgaatta ctaaatgcga cttataatac taacaaatat catgcagaag
      781 ctgtgatgag atttttacac ccaaacttac aaggacaacg attccaattt actggtttaa
      841 cgccaagaga gatgatttat tttggacaaa ctcagggaag aagtccacaa cttcttaata
      901 gaatgactac atataatgaa gttgacaatc ttactaaaaa taataaagga attgctgttt
      961 taggtagtag agtagaatca agaaatggta tgcacgcagg gcatgccatg gcagttgttg
     1021 ggaacgctaa attagataat ggacaagagg ttataattat atggaatcca tgggataatg
     1081 gttttatgac tcaagatgct aaaaacaatg ttattccagt atccaatggc gaccattatc
     1141 gatggtattc atctatttat ggttattaaa atttgtattt tagaaaggta aataatatta
     1201 tggaacaaat tgaattattt agtattgata aattcaaatg taattcagag gctaagtatt
     1261 atcttaatat tattgaggga gaatggcatc ctcaagattt aaatgatagt cctttaaaat
     1321 ttattctcag tacctcagac gattctgatt acatttgcaa atatataaat acagaacaca
     1381 aacaactcac attatataat aaaaataata gctcaattgt tattgaaata tttataccaa
     1441 atgataataa aatactacta acaattatga acacagaagc tttgggaact tctcctagaa
     1501 tgactttcat taagcataag tcataagact atctagatat gattaagtaa actattaatc
     1561 ttattaaagg tctctaggaa gaaaaaattt catgcgatga gattgtttta gaaaaagcga
     1621 cgatatatat aatctaacaa taatttttaa aatattaaga acccccagca tatgctttat
     1681 atagcttgct gggggtttct catacaatat ggatcgagca tcttgcatgt ataaacaaat
     1741 aggctattca aagtaaaatt actcaaactt tgaatagcct ttcctcttca aatagtcttt
     1801 atttaaattg gattatgtta ttggattttc ttacctacaa aaacgatttc ttgatgtgat
     1861 acttcaaccg tgccatcttt ttgctcttga aacacgggtt tttcaatacg ttttaccggc
     1921 atataccctt gcattttcat tcgtgacaaa caatcagcaa ttgtttcatt ctcatttact
     1981 ttgaatttca tgttctacta cactttccag ttcttctggt tcataatctt taagctttct
     2041 acgacgaatg cctttagtcc agaatccacc atgtatgttt cgaggttcat acgcaatgat
     2101 aaatgctttc ggatctaaat tttttatcgt atccatcaat ttacgttcat attttcttgg
     2161 tgttaaaatt tgcatcacca tacgactacc atctctacca aacgcagcat agtgcgtaac
     2221 gccatatcct aaatttcgaa gttcattcgg taaatctaac tcatattctg ctgaagttac
     2281 atttacaact gtataaccta atgccagttt ttcttctatt ttcataccaa caatgatacc
     2341 tattgaaaaa ccaaatgcgt aggcaataat attttgaata tggtctaaat tagacataac
     2401 caaacctaaa ccaacgatat aaactaatac ttctaaaaaa ctaactgatg cagcaatata
     2461 acgataacct ttcaacgtta aaattgttcg catcgttaaa aacgttacat aacaaatgtt
     2521 aatgataaat atagttagta ccattaacca tggattttct gttacgaatg acataaggcg
     2581 ccccactctc tttcaattac actttaaata ccaaactgct tcaacctaac cttaatgaat
     2641 gcatattaat ataatatagt acgaaaactc tcataaataa atagcgaatt tgtcataaat
     2701 aaaaatttat tctcaaatga acatacttac tttagttcat acactttcaa tttatacact
     2761 gaagtagtct agctgaatgt ggtttaatgt tattaattct aactcatatt taatttaagt
     2821 cacacgttag aagaaaaaat taaattagga ttttggccac gtgtatcttg tatatgcaag
     2881 ttcacgtttg tgtgcatttc gtatataatg attttcaatt actttttgtt cttctggcgt
     2941 aactggctta ccttctaaat aattatcaat cgcctcataa gttacaccta atgcatcttc
     3001 atctggaagc tgtggtttat catcttctaa atcagcagtt ggcgtttttt catataattc
     3061 ctttggcgca ccaagatacg ctaataattg acgaccttgt cgtttattca aaccaaatat
     3121 aggtgcgata tctgcagcac catcaccgta cttcgtataa aacccagtta tattttcagc
     3181 tgaatgatct gttcctacta caataccttg tcggtttgaa gcaattgaaa attgtacttt
     3241 catacgttca cgcgcttttt catttccttt ttggaaatct gtaagaacaa taccggcttc
     3301 ttttaatgat tgcacacttt gatcaactgc aggcttaata ttgactgtta ctatttcatc
     3361 tggttcaatg aatcgcaaag cttgctcaac ttcatcagca tctttttgaa ctccataagg
     3421 taatttaact gcaataaacg tacaatcaat gccttcttca cgtaattcgt taacagacat
     3481 ttgtactagt tttccaacta atgtagaatc ctgtcctcct gaaataccta acactaaaga
     3541 ttttataaat gaatgtgatt gtacataatt ttttataaat tgctttaatt ccataatttc
     3601 ttcagcacta tcgatacgct ttttcacttt catttcttgt acaataacgt cttgtaattt
     3661 actcattatc ttcttccatc tccttaacgt gttccgcaac ttcaaaaata cgtttatgtt
     3721 tattatccca acatgccttg cttaaatcga ctggatattc ttgtggattc aggaaacgct
     3781 tattttcatc ccaaatagat tgtaatccta gtgctaaata ttcacgtgat tcatcttctg
     3841 ttggcatttg atatactaat ttaccatttt cataaatatt atgatgcaaa tcaatggctt
     3901 cgaaagattt tataaatttc attttataag tatgcactgg atggaataat tttaaaggtt
     3961 gttcatcgta tggattttca ttttccaaag taatataatc gccttctgcc ttacctgttt
     4021 tcttgtttat aatgcgatat acatttttct tacctggcgt cgtaaccttt tcagcgttat
     4081 ttgataattt aatacgatca ctatatgaac catcttcatt ttcaatagct acaagtttat
     4141 atactgcacc taatgctggt tgatcgtatc ctgtaatcag ctttgtacca acgccccaag
     4201 aatctacttt tgcaccttgt gctttcaaac tcgtaatcgt ttcttcatcc aaatcattag
     4261 acgcgataat tttagtttca gtaaatcctg cttcatcaag catacgtctt gcctctttag
     4321 ataaataagc gatatctcca gaatctaatc gaatacctac aaagttaatt ttgtcaccta
     4381 attcttttgc aacttttatt gcatttggca cgccagattt taaagtatgg aatgtatcta
     4441 ctaggaacac acaattttta tgtctttcag catatttttt gaaggcaaca tattcgtctc
     4501 cataagtttg gacaaatgca tgtgcatgtg taccagacac aggtatacca aataatttcc
     4561 ccgccctaac attacttgta gaatcaaagc ccccgatgta agcagctcta gcgccccaca
     4621 atgctgcatc aatttcttgc gcacgacgtg taccaaactc cattaattta tcatttgatg
     4681 caatttgacg aattctgcta gcctttgttg taattaatgt atggaaattt acaatgttta
     4741 ataaaattgt ttctattaat tgcgcttgaa tcaatggtgc ttctacgcgt aacaatggtt
     4801 cgttaccaaa gcataattcg ccttcttgca tcgaacggat gctgcctgtg aattttaaat
     4861 cttttaaata tgataagaaa tcatccttgt agccaataga ctttaaatat tccaaatcag
     4921 attctgaaaa tccaaaatgt tctataaaat caatgacgcg ttttaaacca ttaaaaacag
     4981 catagccact attaaatggc atttttctaa aatacaaatc aaatacagcc attttttcat
     5041 gaatattatc attccaataa ctttcagcca tatttatttg atataagtca ttatgtaaca
     5101 ttaaactgtc gtcttctaat tggtacactt gtatctctcc aatcgaccta aatattttct
     5161 tacattttat cataattcat ttttttatat acataagagc cccttaattt ccatactttt
     5221 aattaaaatc aaccaacaat ttaatgacat atacataatt tttaagagta ttttaataat
     5281 gtagactata atataaagcg aggtgttgtt aatgttattt aaagaggctc aagctttcat
     5341 agaaaacatg tataaagagt gtcattatga aacgcaaatt atcaataaac gtttacatga
     5401 cattgaacta gaaataaaag aaactgggac atatacacat acagaagaag aacttattta
     5461 tggtgctaaa atggcttggc gtaattcaaa tcgttgcatt ggtcgtttat tttgggattc
     5521 gttaaatgtc attgatgcaa gagatgttac tgacgaagca tcgttcttat catcaattac
     5581 ttatcatatt acacaggcta caaatgaagg taaattaaag ccgtatatta ctatatatgc
     5641 tccaaaggat ggacctaaaa ttttcaacaa tcaattaatt cgctatgctg gctatgacaa
     5701 ttgtggtgat cctgctgaaa aagaagttac acgcttagca aatcacttag gttggaaagg
     5761 aaaaggtact aattttgacg tgttaccact gatttaccaa ttacctaatg agtcagttaa
     5821 attttacgaa tatcctactt cattaattaa agaagtacct attgaacata atcattatcc
     5881 aaaattaaga aaattgaact taaaatggta tgcagtccct atcatttcca atatggactt
     5941 aaaaatcggt ggcattgtat atccaactgc accctttaac ggttggtata tggtaactga
     6001 aattggcgta cgtaacttta ttgatgatta ccgttacaat ttactagaaa aagttgcaga
     6061 tgcgtttgaa tttgatacac ttaaaaataa ttcatttaat aaagatcgag cacttgttga
     6121 attgaactat gctgtgtatc attcctttaa aaaagaaggc gtatcaattg tcgatcattt
     6181 gaccgctgca aagcaattcg aactattcga acgtaacgaa gcacaacaag gtcgtcaagt
     6241 taccggaaaa tggtcttggc tagcaccgcc attatctcca acattgacgt caaattatca
     6301 tcacggatat gacaatacag taaaagatcc aaactttttc tataaaaaga aagaatcaaa
     6361 tgctaaccag tgccctttcc atcattaagt tagtagaggt gtagcatatg caattatatt
     6421 atttaggccc taaaggtaca ttttcatatt tggcttgtcg tcagtatttt tctgaaaatg
     6481 aagcaacttt tcaaccaaag tcaaattttt ttgaagtcat caaggcagtt gcagatgatg
     6541 acacatcgat tggtgttgtc cctattgaaa attctataga aggaacaatc aacattgtgg
     6601 cagatgcact tgcacagcaa gatgtctttg cacacggtga aatacgttta gatattaact
     6661 ttgcattata tggtaatgga acagattcca tttctgatat taaaaaagtg tattctattg
     6721 caccagcaat cagtcaaaca acaaattaca tacatcaaca tcaatttgac tatgattatg
     6781 tcgacagtac aattcaaagt ttgactaaaa ttgaaaatgg cgttgcagca attgcacctt
     6841 taggtagtgg cgaagcttat ggttttacac ctattgatac acatattgaa gactatccac
     6901 ataacgtgac acgtttttta gttataaaaa atcagcagca atttgatcaa aatgcgacat
     6961 cattgatgtt tttaattaca ccaatgcatg ataaacctgg gttgttagca agtgtgctta
     7021 atacatttgc tttgtttaat attaatttat cctggattga gtctcgtcct ttaaaaacac
     7081 aattaggtat gtatcgattt ttcgttcagg ctgattctgc aattacaacc gatataaaga
     7141 aagtcattgc tattttagaa accttagatt tcaaagtaga aatgattggt gcttttaatt
     7201 aaactttatt ttctaggttc tacaatttaa ataaaataaa gagtcaacca aggattaaaa
     7261 taggaatcca tccttggttg actttctttt gtttgtattg tttttacacg atgctgcggt
     7321 atacgtttcg ttaccactcg tccaagcaat tgctaatgta accttttatt tttcgttcta
     7381 atctgcaatt actatttcaa tggcagtggt tgatttatat caatacctaa aactataggc
     7441 attacataat acacgactaa aataataatt attgcactga ttaagtttac ccagaatcct
     7501 actgatgcca tttgtttgat agatatttta ccagaaccaa agataattgc attcggtggt
     7561 gtccctactg gtaacatgta tgcacagtta gccgccatag ctgcaggtgc cataagtagt
     7621 aatggatgca ctccaacagc aacagacaac gttgctaaaa tcggtaaaat catcgttgca
     7681 gttgcagtat tagatgtcac ttcagttaaa aataagacaa agattgttat gacaattaca
     7741 ataagaatcg gactaacacc atttaatgat ttcaactgtt cgcctaacca ttttgctaaa
     7801 ccactttcag aaataccttt cgctaatgct aaaccgccac caaataaaat taatacaccc
     7861 caagggagct cttttgcaac ttcccagtca atgatacggc gatgtttttc agtattttta
     7921 gctggaataa taaataataa tattgatata aaaatagcaa tcgtaccatc tgcaacagat
     7981 gacgtaactt cccatttttt cagaagaaac tctcttgtaa tccataataa gctagcaagt
     8041 acaaagatag tttgtactac cttttcttca tacttcattt tgccaagctc gtctaacttt
     8101 tgtttaatta acgtctgacc accaggtaaa tatttcaaat catgtctaaa cgcaacatat
     8161 cttaaataga gccaagtaat acctaacaaa acaatgaccg ttggaatccc tacaatcatc
     8221 catttagcaa aactaatttc atatccaaaa tgttgcatgt attgtccttt taaaataatt
     8281 aatggcgggg ttccgattaa tgtacccaag ccaccaatcg tacctgcata gccaattgct
     8341 aaaactagag atttttcaaa cttttgaata cttgtttgat tcgtattggc ttcttgtaaa
     8401 tcatgtgctt ccttaataat tgctaaacca atcggaatca taatcattac agctgcagtg
     8461 ttcgatacaa acatagataa gaatcctgtt gccaccatga atccaagtaa tatttttgaa
     8521 gtgcttgcac caatcaaatt aataattgtc aacgctactc tcgtatgtaa attccatctt
     8581 tccattgcaa ttgccaaaat aaatccacct aaaaacaaaa agataatatc attgccatat
     8641 tcggatgata cttgttctgg tgtaagtata tgacctaatg gtaataacac aattggtaat
     8701 aagctcgttg ctgcaatagg aattgcttca gtaatccacc aagtcgcaat ccataaagta
     8761 atcgctaaaa catagacgcc tttccaaggt aagtcttgtg gatgaaagaa taataatgtt
     8821 aataggaaaa gtaatggacc taatattagt ccaataagtt gtcccgcact aaacggtttc
     8881 tttttctttg attttgaaaa gaatgttaaa taccttttcg atatcattga ttgatgttga
     8941 ttgaaataag ccataaattt ccccctcatg atgattatga caagctactt caactcacta
     9001 tattagagta tagaaagttg tctatcttgt attatcatta ccgtcaaaat acccctttta
     9061 tcagataatt gggtttatta tagcatattt ataccgtttt atgaatgcgc tttcaaaaat
     9121 tatctactat tcttaatcct aatgtcatgc gaaacttttt acttttttat ttcatctaca
     9181 ttgattgtag taacttgaat acccgcttca acaggactta aaaacactgt atcaatatct
     9241 tctaaatgaa ttgattgagt cgaattattg aatgtattcc caacgattaa tacatcttta
     9301 tgtttaacat tattataatt acgtacatgt atcgcatctt tagacattga tcctttaaat
     9361 tcatttccaa ttatattcat atttatgcct gcttgggaac ctaagtcttt tcctgtcatc
     9421 acatctgctg catttttacc atctctaact cctaaatatc taatgccacc ctcacagtta
     9481 ataaacttat tattaataat gaaagcatct ttatacttca agggagttaa tgcataacct
     9541 tgtatatctt caaatatatt atttctaata tgaatattct catagtatcg attatgtcta
     9601 cttgcatgtg agccaatagc acgattccaa cttcccattt cgggcaattc tgaaggtcca
     9661 aaataacaat cttcgataat gacatttttc gttatcgtac catctgtcgt tccgaatttt
     9721 ggaaaagcac caggtacttg aatgtctaat tgtattgctt cagaataaaa gcgttcgcca
     9781 ctatagtcta tgaatccttc aaatgaacag cttttaatat agagtccgtt aatcccacaa
     9841 gcatcaattg catgaccact cactacattc ttaatggtca cacctattaa ttgaatatct
     9901 tcagcatgcc caatgcacat agctgtattg ttataaggat acgatacacc attcatatca
     9961 aacttgccgc ctttaatatg aatatgactg tgtccattat aaccacgata aaaaccaaag
    10021 cgacgaccat tttttaataa aggaccagaa tgacatcggc gtaaaatagt ttcattatct
    10081 aacaaaagtg ttgtattgcc atatatcgtt aatggtttgc aaatatcata cgtccctttc
    10141 ggtatataaa ctgtcgttgg tttacatctt ccacgattta acgcacgttg tattgctcga
    10201 gtatctttag ttttgtttat gcctgttaac ccataatctt tcatattgat atacataaat
    10261 cgtcatcctt tgtctatttt ctaattactc atatattaac caattattca aattataatc
    10321 gtgttaggtt gatttaacag atagtcataa atctcgtgat gccatctatt agcattttta
    10381 tattcaaaac atcaacactt gatattagtt ctaagtttgt cacatttagt attctattac
    10441 agctatttta aaactatcgc aaaaacttct tatattattt gttgattgcg tgatttgctc
    10501 ttccaaaaaa gttgattaat ctatatactt aatctatatt tatatattaa ccattagggt
    10561 taaaaattac tctagcattt atgaatagat gggagtttat tttattatta tataggagag
    10621 atgttgaatg acacatcgcg cactattagt tgttgactat tcatatgact ttatcgcaga
    10681 cgacggctta ctaacatgcg gtaaacctgg acaaaatatt gaagatttta ttgtttctcg
    10741 tatcaatgac tttaattatt atcaagacca tatattcttt ttaatggatt tacattattt
    10801 acatgacatt catcatcctg aaagtaaatt attcccacca cacaatatcg tagatacaag
    10861 tggtagagaa ttatacggta aagtaggtaa attatacgaa acaattaaag cgcaacctaa
    10921 tgtacatttc attgataaaa cgcgctatga ttcgttcttt ggtaccccgc ttgatagttt
    10981 attgagagaa agaagtatta atcaagtcga aatcgttggt gtatgtaccg atatttgcgt
    11041 gttacataca gcaatttctg catacaactt aggttataaa atttcagtac ctgctgaggg
    11101 agtggcttca tttaatcaaa aagggcatga atgggcactt gcacatttca aaaactcatt
    11161 aggtgcagag gtagaacaac acgtttaaat cgtgctaaaa taattataaa gaatacaatt
    11221 tacaagggag atatttgaca atggctaaaa catatatttt cggacataag aatccagaca
    11281 ctgatgcaat ttcatctgcg attattatgg cagaatttga acaacttcga ggtaattcag
    11341 gagccaaagc ataccgttta ggtgatgtga gtgcagaaac tcaattcgcg ttagatacat
    11401 ttaatgtacc tgctccggaa ttattaacag atgatttaga tggtcaagat gttatcttag
    11461 ttgatcataa cgaattccaa caaagttctg atacgattgc ctctgctaca attaagcatg
    11521 taattgatca tcacagaatt gcaaatttcg aaactgctgg tcctttatgt tatcgtgctg
    11581 aaccagttgg ttgtacagct acaattttat acaaaatgtt tagagaacgt ggctttgaaa
    11641 ttaaacctga aattgccggt ttaatgttat cagcaattat ctcagatagc ttacttttca
    11701 aatcaccaac atgtacacaa caagatgtta aagcagctga agaattaaaa gatattgcta
    11761 aagttgatat tcaaaagtac ggcttagata tgttaaaagc aggtgcttca acaactgata
    11821 aatcagttga attcttatta aacatggatg ctaaatcatt tactatgggt gactatgtga
    11881 ctcgtattgc acaagttaat gctgttgacc ttgacgaagt gttaaatcgt aaagaagatt
    11941 tagaaaaaga aatgttagct gtaagtgcac aagaaaaata tgacttattt gtacttgttg
    12001 ttactgacat cattaatagt gattctaaaa ttttagttgt aggtgctgaa aaagataaag
    12061 ttggcgaagc attcaatgtt caattagaag atgacatggc cttcttatct ggtgtcgttt
    12121 ctcgaaaaaa acaaatcgta cctcaaatca ctgaagcatt aacaaaataa tactatatta
    12181 ctgtctaatt atagacatgt tgtatttaac taacagttca ttaaagtaga atttatttca
    12241 ctttcaatga actgtttttt atttacgttt gactaattta caaccctttt tcaatagtag
    12301 tttttattcc tttagctacc ctaacccaca gattagtgat ttctatacaa ttcccctttt
    12361 gtctttacat tttcttaaaa tatttgcgat gttgagtata aatttttgtt ttcttcctac
    12421 ctttttcgtt atgattaaag ttataaatat tattatgtac acgattcatc gctctatttt
    12481 caactttcaa catatataat tcgaaagacc atttaaaatt aacggccaca acattcaaat
    12541 caattaatcg ctttttccaa aataatcata taaggaggtt cttttcatta tgaatatcat
    12601 tgagcaaaaa ttttatgaca gtaaagcttt tttcaataca caacaaacta aagatattag
    12661 ttttagaaaa gagcaattaa agaagttaag caaagctatt aaatcatacg agagcgatat
    12721 tttagaagca ctatatacag atttaggaaa aaataaagtc gaagcttatg ctactgaaat
    12781 tggcataact ttgaaaagta tcaaaattgc ccgtaaggaa cttaaaaact ggactaaaac
    12841 aaaaaatgta gacacacctt tatatttatt tccaacaaaa agctatatca aaaaagaacc
    12901 ttatggaaca gttttgatca ttgcaccatt taactatcct tttcaactag tgttcgaacc
    12961 tttaatcggt gctattgcag caggtaatac ggcaattatt aaaccatctg agttgacgcc
    13021 taatgttgca cgagtgatta aacggttaat caatgaaaca tttgatgcaa attacattga
    13081 agttattgag ggaggaattg aagaaacgca aacgttaatt cacttacctt ttgactatgt
    13141 cttctttaca ggaagtgaaa atgtaggcaa aatcgtttat caagctgcca gcgaaaattt
    13201 agttcctgtg acattagaaa tgggcggtaa atctccagtc atcgttgatg aaacagcgaa
    13261 tattaaagtt gctagtgagc gcatttgttt tgggaaattc actaatgccg gccaaacatg
    13321 tgttgcacca gattacattt tagtacacga atctgtaaaa gatgatttaa tcacagccct
    13381 atcaaaaacg ttgcgtgaat tttatggtca aaatatacaa caaagtccag attatggccg
    13441 cattgtaaac cttaaacatt atcatcgtct gacttcatta cttaacagtg cacaaatgaa
    13501 tattgtattt ggtggtcata gtgatgagga tgaacgttat atagaaccaa cattgttaga
    13561 tcacgttaca agtgattcag caattatgca agaagaaatt tttggtccta tcttaccgat
    13621 tttaacgtat cagtcattgg atgaagcaat agcctttatt caccaaagac caaaaccttt
    13681 gagtttatat ttatttagcg aagatgaaaa tgctacacaa cgtgtaataa acgagctatc
    13741 atttggcggc ggcgctatta atgatacatt gatgcaccta gcgaatccta aattaccatt
    13801 tggtggtgtt ggcgcctcag gtatgggacg ctatcatggt aaatattcat tcgacacttt
    13861 tacacatgaa aaaagctaca ttttcaaatc tacacgatta gaatcaggtg tccatttacc
    13921 accatataaa ggtaaattta aatacatcaa agctttcttt aaaaattaat ttttgagaca
    13981 aaaataaacc atgcttccat aatttgatgt tcttgaaaac aataaatttc aaggtataca
    14041 caaatttttt ggaaacatgg ttttcttttt atcatgatta attaaagatt aaaatatgac
    14101 acatacacct tcagatgcta cacgagtgtt atcacatagc gtaattttac ctgtttcttt
    14161 atttctttcg aaaacagtta caactgaatc tccttgctca tgagcacaaa ctaaaaggtc
    14221 atctgatgag gcaatattaa aatctcttgg gaattgacca ccactttcag taattgttac
    14281 tagttctaag tgttgaccat tatcaagaac tttaaaaatt gcaatgctat catgccctct
    14341 attagataca tataagaatt gttgatcatg agataaacgc actgctgcaa gtttagtatc
    14401 tccatcaaag ttttcaggaa ttgttaaatg acgctcgagc tcttcaaatt taccgtcatt
    14461 atattctgca acactcacag tatttgataa ttcgtgtacg acataagcaa attttccatt
    14521 atcatgaaat tcaatatgtc ttgtcccatc actatcttta aataaagatt ctttataaaa
    14581 ttcaaacccg ttgtcatcaa atttataagt aacgatacga tcagcaccta aatctgttac
    14641 tgcaacgtac ttaccatctg gagtttgatt aatataatgt gcgtgtggat gatcttgtct
    14701 ttcatgtgta cctgttggaa aatcatgtgc aagttcttga attagacgta taatttcacc
    14761 tgtgtgcgta tttaattcat acatacgtat gatgccagca ccatataccg cttcaaataa
    14821 atatcgttta tcttctgaaa tcgatacata acaacctgta ccagcttttg aagacaaaca
    14881 tttatttaat aaatgcaatt caccattgtc atcaattttc aaacttgcaa caccgcattg
    14941 ttctccttct ttgttgattc catataaaac ttcattatta cgcaccaaat atgtagacgc
    15001 ttctaattca aatcctgttt ctaataaatc aatacgtgac tgattttcgt ttaattcaaa
    15061 acgataaatc cctttaccat tctttttagt gtaagaacca atatatccat ttgtcattac
    15121 ttttacccct ccatgttagt cttattttca ttttattaaa ttttagctag aattaacaat
    15181 catttatcat ttccttaaag ctaaaactat ttaactactt taaaagaact atttgctgtg
    15241 atatgtcatt attcgatttt aattaatccc tcttttttac ttacacgagc attaattatt
    15301 aacgcgttta tgcttccaca aaatttttgt tgcaaaatag aacacatgtt cgtataatgt
    15361 aattatcaga ttggggtgaa ttaaggatga caagttatga agaagaaact gattatcgta
    15421 acgttccaag agaattacta gataacaata tacctatggg aagaggcatg attaaatggg
    15481 ctccttttgc aacattaccc gaacaattcg aaacgattca acaatacatt atcgaccaaa
    15541 acaaaattac acgtcctgta ttatcagatg atcaattggc tgagctaaac attcggctac
    15601 acgaggcgct acaatatgcc caacctgtag aagttaaatt ttataataat ggctttgtag
    15661 attcagtacg cttaaccatt tatcgtattg atgctattaa ttatgaaatt gatggttatg
    15721 tttacaatca gcaacaacgt caaaaaattt ctatctttga tatcttagat attgtcttgt
    15781 taccttaatg ctttgaacat aattaaacag attgagtata tgcatataag atgattttta
    15841 acatcatttt tggatgatag gatgttgcgc cacgatgatg tctgaattca tcgaattcgc
    15901 tatcaggtat tgtttcaaca atttcattaa catatcgtga aatatcattt tgagggattc
    15961 taaca
//
LOCUS       DALAAR010000057        15799 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000057 DALAAR010000000
VERSION     DALAAR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.059"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(38..199)
                     /locus_tag="O2458_001950"
     CDS             complement(38..199)
                     /locus_tag="O2458_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887811.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(236..976)
                     /locus_tag="O2458_001951"
     CDS             complement(236..976)
                     /locus_tag="O2458_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCZ3887812.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1182..1973
                     /locus_tag="O2458_001952"
     CDS             1182..1973
                     /locus_tag="O2458_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ3887813.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2341..2769
                     /gene="lacA"
                     /locus_tag="O2458_001953"
     CDS             2341..2769
                     /gene="lacA"
                     /locus_tag="O2458_001953"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCZ3887814.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2785..3300
                     /gene="lacB"
                     /locus_tag="O2458_001954"
     CDS             2785..3300
                     /gene="lacB"
                     /locus_tag="O2458_001954"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCZ3887815.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3313..4245
                     /gene="lacC"
                     /locus_tag="O2458_001955"
     CDS             3313..4245
                     /gene="lacC"
                     /locus_tag="O2458_001955"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCZ3887816.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4249..5229
                     /gene="lacD"
                     /locus_tag="O2458_001956"
     CDS             4249..5229
                     /gene="lacD"
                     /locus_tag="O2458_001956"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCZ3887817.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5251..5562
                     /locus_tag="O2458_001957"
     CDS             5251..5562
                     /locus_tag="O2458_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCZ3887818.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5568..7280
                     /locus_tag="O2458_001958"
     CDS             5568..7280
                     /locus_tag="O2458_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ3887819.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7298..8710
                     /gene="lacG"
                     /locus_tag="O2458_001959"
     CDS             7298..8710
                     /gene="lacG"
                     /locus_tag="O2458_001959"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCZ3887820.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            8976..9845
                     /locus_tag="O2458_001960"
     CDS             8976..9845
                     /locus_tag="O2458_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3887821.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10128..11129
                     /locus_tag="O2458_001961"
     CDS             10128..11129
                     /locus_tag="O2458_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCZ3887822.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11363..12364
                     /locus_tag="O2458_001962"
     CDS             11363..12364
                     /locus_tag="O2458_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCZ3887823.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            12671..14233
                     /locus_tag="O2458_001963"
     CDS             12671..14233
                     /locus_tag="O2458_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ3887824.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14408..14944
                     /gene="amaP"
                     /locus_tag="O2458_001964"
     CDS             14408..14944
                     /gene="amaP"
                     /locus_tag="O2458_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCZ3887825.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            14957..15196
                     /locus_tag="O2458_001965"
     CDS             14957..15196
                     /locus_tag="O2458_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCZ3887826.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15259..15768
                     /locus_tag="O2458_001966"
     CDS             15259..15768
                     /locus_tag="O2458_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ3887827.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         5574 a         2332 c         3024 g         4869 t
ORIGIN      
        1 ttacaaatga caaaccatta aaagtagcat cccaacatca aacaaaaaaa caattcatca
       61 aataaaaatc gctacaaaac caagtcatta aacacgcaat aattaaaatt ttccactcat
      121 tataattctg aattccaaat gtcgaattcc gaaaaccaaa ctccaaattc caaaaacgca
      181 actccaaaat taaaagcatt tccctaccat tcgggaaatg ctttttacat actgattact
      241 ctgtcattaa tgattttaca acggaaacca tgtcgtcatg tatgaccaaa gtagcgtcgc
      301 tatcataagg tgttcgatct ttattgataa taattaaatt gtcgccttta aaatgtgata
      361 ttaatcctgc ggcaggttgt acaacgagtg atgaacctag tacaacaagg gtgtcagcat
      421 gttcaatttt atttaatgcc cttatgatgg taggttgatc taacatttca ccgtataata
      481 cgatgtccgg tcgaatggca ccaccacaat tatcacaatg tttcaaagtt ctatcaataa
      541 catctgactt cgtataagat ttatgacata cattacaata aaaacgattt aacgtgccat
      601 gtaattcatc aacatgttga cttccagcgt ctgagtgcaa accatcgata ttttgcgtga
      661 tgacacctaa agattgttga ttacgttcta attttgcaat ccaatcatga acgatattgg
      721 gcatcgtatc gacaaatagt aagcgcttat ggcagaaatt gataaaacct tcaggatcat
      781 cttctaaata atcacggctt aacaagtatt ctggcgaaag cccatctttt gaaatttcat
      841 caaataagcc acccattgaa cggaaatctg gaacgccact tgcgacagat acaccagcac
      901 ctgtaaaaaa tgtaatacga ttcgaactat ctataatatg ttttagtgtc tctaaatcat
      961 gtttcaagtt aaacacccca tcaaaataat gaatgattta taacgtcttt tacttttatt
     1021 atagtatatt cattttacga ttatcaaaag cgcagtttaa acagttcttt ttaaaaatga
     1081 attatacata taatcgaaca gttaaattat attagttttt acgcaatttt tattatagaa
     1141 taagcatgta tattaaaatt gatgtctaaa cataaaggat aatgacccat cgcctaaata
     1201 aaggaggcaa ttttacattg aataaacatg aacgtttgga cgaaattgct aaactagtga
     1261 ataaaaaggg cacgataaga acgaatgaaa tcgtcgaagg tttaaatgtg tctgatatga
     1321 cagttcgaag agatttaatt gaattggaaa ataaagggat tttaacgaag attcatggtg
     1381 gtgcacgcag taattcaaca tttcagtata aagaaatatc gcataaagaa aaacataccc
     1441 gtcaaattgc agaaaaacga tttatagcta aaaaagctgc atcattaatt gaagatgggg
     1501 atactttgtt tttcggacca ggaacaacag tggaactatt agcagaagaa gtcaatcatc
     1561 atacgctcac aattattacg aattgtttgc cggtgtataa aattttgtta gaaaaacaaa
     1621 cagcacattt tcgtgtctat ttaattggtg gtgaaatgcg ccatattaca gaagcatttg
     1681 taggtgaaat ggcgaacgcc atgttggaaa aactaagatt tagcaagatg ttctttagta
     1741 gtaacgcagt aaataaaggt gctgtgatga catctacatt agatgaagcc tatacgcaac
     1801 aactggcact aagtaattca attgaaaaat acttgttaat cgatcatacg aaagttggca
     1861 aagaagattt tacatcattt tgtcagttaa acgaattgac tgcagtggtc atggactatg
     1921 aagatgaaga aaaagtagaa acgattaaaa catatattga agtagtcgac taaattaaaa
     1981 gtataactgc attgttaaaa tatgtatcta tatgctcata agtgaatgtg aatttgcatt
     2041 catttatggg ctatttttta tgcaaattta tgtcaacggg aacctcgaca aagttttact
     2101 acaatttcag acgaatacct aattaaaaca acaccacaat atgaatgttc acttattgtt
     2161 tgaaaatacg tcataaacaa acatattata aacatattat gttgatttat tgtttgttta
     2221 tgtttataat ttaaacgtaa tcaaattgaa agccttttct caagattttc ataacctaca
     2281 aataaatatg tcacatttta agaaaggtat ttcaaaatta aagtaaaaag gagtcttatt
     2341 atggcgatta ttattggttc agatgaagct ggcaaacgat taaaagaagt catcaaatca
     2401 tacttattag acaataaata tgatgttgtt gacgtaacag aaggacagga agttgacttt
     2461 gttgatgcaa ctttggctgt agcaaaagat gttcaaagtc aagaaggtaa cttaggtatt
     2521 gttatcgatg catttggtgc aggtagcttt atggttgcta caaaaatcaa aggcatgatt
     2581 gctgctgaag tatcagatga acgctctgga tatatgacta gaggccataa caattcaaga
     2641 atgatcacta tgggatctga aattgttgga gacacattag cgaaaaatgt cgttaaaggt
     2701 tttgttgaag gtaaatacga tggtggtaga caccaaatcc gcgtagatat gcttaataaa
     2761 atgtgctaag gaggaaaaat tactatgaag attgcattag gatgcgacca tattgttaca
     2821 gatacaaaaa tgcgtgtatc tgaattttta aaatcaaaag gacatgaagt cattgacgta
     2881 ggaacatacg atttcacaag aacacattat ccaatttttg gtaaaaaagt tggcgaacaa
     2941 gttgttagcg gtaatgcaga cttaggtgtt tgtatttgtg gaacaggtgt tggtattaac
     3001 aatgctgtaa ataaagtacc tggcgttcgt tcagcactag tacgtgatat gacatcagcg
     3061 ttatacgcta aagaagaatt aaatgcgaac gttattggct tcggtggacg tattataggt
     3121 gagttattaa tgtgcgatat tatcgatgca tttattaatg ctgaatataa accaactgaa
     3181 gagaacaaaa aattaatcgc taaaattaaa catttagaaa caagcaatgc agatcaagct
     3241 gatccacatt tctttgatga attcttagaa aaatgggaca gaggcgaata ccacgattaa
     3301 gggacgacac atatgatttt aactttaaca ttaaatccat ccgttgatat ttcataccca
     3361 ttaacagcat tgaaattaga tgatgttaat cgtgttcaag aagtaagtaa aacagctggt
     3421 ggtaaaggtt taaatgtaac aagagtactt gcgcaagtag gtgagccagt ccttgcgagt
     3481 ggttttattg gcggtgaatt aggtcaattt attgctaaaa aattagatca tgccgacatc
     3541 aagcatgcat tttataacat taagggtgaa acacgaaact gcattgcaat tttacatgaa
     3601 gggcaacaaa cagaaatttt agagcaggga ccggagattg ataatcaaga ggctgcagga
     3661 tttattaaac attttgaaca attattagaa aaagttgaag cagttgctat ttcaggtagc
     3721 ttacctaaag gattaaatca agattattat gcacaaatca ttgaacgatg ccaaaacaaa
     3781 ggtgtgcctg tgatattaga ttgctcgggt gcaactttgc aaacagtttt ggaaaatcca
     3841 tacaaaccta cagtcattaa accaaacatt tctgaactat atcaattgct taaccagcca
     3901 cttgatgaaa gtttagaaag tttgaaacag gcagtgtcac aacccttatt cgaagggatt
     3961 gagtggatta ttgtgtcttt aggtgcacaa ggtgcattcg ctaagcacaa tcatacattc
     4021 tatagagtga atattccgac aataagtgta ttaaatccgg ttggttcagg tgattctaca
     4081 gttgctggaa ttacttcagc aattcttaat catgaaaacg accacgattt actaaaaaaa
     4141 gccaatactt taggcatgct gaatgcacaa gaagcacaaa caggttatgt caatttaaat
     4201 aattatgatg atttattcaa tcaaattgaa gttttagagg tgtaaaaaat gtcaaaatcg
     4261 aatcaaaaaa tcgcatcaat tgaacaatta agtaacaacg aaggtatcat ttctgcatta
     4321 gcatttgacc aacgtggtgc tttaaaacgc atgatggcaa aacatcaaac tgaagaacca
     4381 actgtagctc aaattgaaca attaaaagta ttagttgcgg aagaattaac acaatatgca
     4441 tcttcaattt tattagatcc tgaatatggt ttaccagctt cagatgcacg taacaaagat
     4501 tgtggattat tacttgctta tgaaaaaact ggatatgacg taaacgctaa aggtcgttta
     4561 cctgactgtt tagtagagtg gtctgcaaaa cgtttaaaag aacaaggcgc aaatgctgtt
     4621 aaattcttac tttactatga tgttgatgat gctgaagaaa ttaacattca aaagaaagca
     4681 tacattgaaa gaatcggttc agaatgtgtt gcagaagata ttccattctt tttagaagta
     4741 ttaacatacg atgacaacat tcctgataac ggtagtgttg aattcgcaaa agttaagcct
     4801 agaaaggtta acgaagcaat gaaattattc tcagaaccac gctttaacgt tgatgtattg
     4861 aaagttgaag taccagtaaa catgaaatat gtagaaggct ttgctgaagg tgaagtggtt
     4921 tatacaaaag aggaagcggc acaacacttt aaagatcaag atgcagcaac tcatttacca
     4981 tacatttact taagtgctgg tgtttctgcg gaattattcc aagaaacttt aaaatttgct
     5041 cacgaagctg gtgctaaatt caacggggta ctttgtggcc gtgctacatg gtctggtgct
     5101 gttcaagtat acattgaaca aggtgaagat gctgctcgtg aatggttacg tacaactggt
     5161 ttcaaaaata ttgatgactt aaataaagta ttaaaagata ctgcgacatc ttggaaacaa
     5221 agaaaataag tttaaaaggg gattaatgct atgaatagag aagaagtcca attattaggt
     5281 tttgaaattg ttgcatttgc aggggatgca cgttctaagt ttttagaagc attaacagca
     5341 gctcaagctg gagattttgc aaaagcagat gcactgattg aagaaggaaa caattgcatt
     5401 gcggaagcgc atagagcaca aacaagcctg ttggctaaag aagcgcaagg tgatgatatt
     5461 gcatacagtg taacgatgat gcatggtcaa gatcatttaa tgacaacaat tttactgaaa
     5521 gatttaatga agcatttatt agaattttat aaaagagggt gatgacaatg atgcaaaaat
     5581 tgatcgcaca aattgaaaaa gggaagccat ttttcgagaa attatcacgt aacatatatt
     5641 taagagccat ccgtgatgga tttatttctg caatgcctgt catcttattc tcaagtatct
     5701 ttctattaat tgcatatgtg ccaaatatat ttggttttaa atgggataaa ggcatggaag
     5761 ccattttaat gaaaccatac aactatacga tggggcttgt agcattttta gtagcaggaa
     5821 cgacagcgaa atctttaaca gattcattta accgtaagct agaaagtaca aatcaaatta
     5881 actttatttc tacaatgcta gcagcgatgt gtggattctt gttcttagct tctgatccag
     5941 ccaaagatgg tggattctta agtgcattta tgggaactaa aggtttatta acagcattct
     6001 tatcagcatt tgtaactgtt attgtttata acttctgtgt gaagcgcaat attacaatta
     6061 aaatgccgaa agaagtaccg ccgaatattt cacaagtatt taaggactta attccatttt
     6121 cagcggtaat catcattctt tatgcattag atttagtcat tcgcaacagc tttaaatcaa
     6181 atgtagcgga aggtatttta aaattattcg aaccattatt tacagcagca gatggatgga
     6241 ttggtgtcac aattatcttt ggtgcctttg cattattctg gtttgtaggt attcatggtc
     6301 cgtcaattgt agagccagca attgcagcca ttacatatgc gaatatcgaa gcgaacttca
     6361 agttgcttca agctggagaa cacgcagata aaattattac atcaggtaca caaatgttta
     6421 tcgttacatt tggaggtacg ggtgcaacat tggtcgtccc gttcatgttt atgtggatga
     6481 cgaaatctaa acgaaacaaa gcgattggac gtgcatcagt tgtaccaaca ttctttggtg
     6541 taaacgaacc aattttattt ggtgcaccac tagtattgaa tcctgtgttc tttattccat
     6601 ttgtattagc accaattgtt aacgtatgga ttttcaaact tttcgttgaa gtgttaggaa
     6661 tgaatagttt tagtgtgaat ttaccttgga caacaccagg tccattgggc attatcatgg
     6721 gtacaggttt tggtttatgg tcattcgtac tagctattac tttgattgtt gtagatatta
     6781 ttatttacta cccattccta aaagtttatg atagtgaaat tcttgatgaa gaagaaggac
     6841 gtaaagaaag taattcagat ttaaaagaaa aagttgcagc aaactttgat acgaaaaaag
     6901 ctgattcaat tttagcggca agtggtgtat cagacgatgc agctaaagct tcaaatatca
     6961 cagaacaaac aaatgtctta gtactttgtg ccggtggtgg tacaagtgga ttattagcca
     7021 atgcacttaa taaagcagct gaagaatatc atgtgccagt gaaagcggca gctggcggat
     7081 atggcgcaca tatggacatt atgaaagagt accaattaat cattttagcg ccacaagttg
     7141 cgtctaacta tgaagatatt aagcaagata cagatagatt aggtattaaa ttagcgaaga
     7201 ctcaaggtgc tgaatatatc aaattgacac gagatggcca agcagccctt gatttcgttc
     7261 aacaacaatt tgaaaattaa gaataggagt ttttcatatg actaaaacat tacctgaaga
     7321 ttttattttt ggtggagcaa cagcagcata tcaagcagaa ggtgcgacaa atacggatgg
     7381 taaaggtcgc gtagcatggg atacgtattt agaagaaaac tattggtaca ctgcagaacc
     7441 agcgagtgat ttttataaca gatatccagt ggatttagaa ttaagtgaaa aattcggtgt
     7501 taatggtatc cgaatttcaa ttgcatggtc tcgtatcttc ccaaatggat atggcgaagt
     7561 taaccctaaa ggtgttgagt attaccataa gttatttgca gagtgtcata aacgtcatgt
     7621 tgaaccattt gtaacattgc atcattttga tacaccagaa gtgttacaca aagatggcga
     7681 ttttctaaat cgtaaaacga ttgactactt tgtagattat gctgaatatt gttttaaaga
     7741 attcccagaa gtaaaatatt ggactacatt caatgaaatt ggacctattg gtgatggtca
     7801 atatttagta ggtaaattcc ctccaggtat taaatatgat tttgaaaaag tattccaatc
     7861 acatcataac atgatggtgg ctcatgctag agcagtaaaa ttatttaaag atggcggata
     7921 caaaggagaa attggtgttg tacacgcatt gccaacaaaa tatccgttcg atccatcaaa
     7981 tcctgaagat gttcgtgcag cagaattaga agatatcatc cataataaat tcatcttaga
     8041 tgcaacatat ttaggtaagt attctcgtga aacgatggaa ggtgtgcaac atatcttatc
     8101 tgttaatggc ggtaaattaa acattacaga tgaagattat gccattttag atgcagccaa
     8161 agatttaaac gacttcttag gtatcaatta ctacatgagt gattggatga gaggttacga
     8221 tggagaatct gaaatcacgc ataatgcgac aggtgataaa ggcggttcta aataccaact
     8281 taaaggtgtc ggacaacgtg aatttgatgt cgacgtacct cgcacagact gggactggat
     8341 gatctatcct caaggtttat atgatcaaat catgcgtgtt gttaaagatt atcctaacta
     8401 tcataagatt tatatcactg aaaatggttt aggatataaa gatgaattta ttgaatctga
     8461 aaaaacagtt catgatgatg cacgtattga ttatgtaaga caacatttga acgttattgc
     8521 agatgctatt atagatggtg caaatgttaa aggttacttt atttggtcat taatggatgt
     8581 gttctcttgg tcaaatggtt atgaaaaacg atatggttta ttctatgtcg attttgagac
     8641 tcaagaacgt tatcctaaga aaagtgctta ttggtacaaa gagttagccg aaacaaaaga
     8701 aattaaataa ttatagatat taaaaggcct atcagtcgag tgctggtagg tctttattta
     8761 tagaaagaga taaattaaac atgcatatac atatttatat attaatcaat gggtgacaca
     8821 ccattgaaag ctaaagaagc aatcaattaa caaacgttaa cggacaactt tccaaagtca
     8881 tcttaaaagt taatgatgta ttcaaaatca actaaaggac atttttaatc acatccttta
     8941 tacttatata aaagacatga atgggagtta aaagtgtgaa gaaaattttt gtaattatta
     9001 cgacattgtt agcggtcgct attattatag gctctattat tatggtggtg tttagtcaac
     9061 gacaggcaca aacatttaaa atacaacaac agcaatttgt taaaaagcca attccaacat
     9121 tgttcttaca tggatttggt ggtagtgcca attctgaaaa gtttatggtg aagcaagcag
     9181 aaaaaagagg ggtaactaaa gatatcatca ctgcttacgt ttcaaaggat ggtgcagtga
     9241 catttaaagg gaaactaaga aaagatgcag tgaatcctat tgtgaaaatt gaacttgaaa
     9301 ataacagaca aggctatttg gataagaatg ctgcgtggtt taaaaatgta ctgactaagt
     9361 tacaatctga atacaatttc gataaattca actttgtcgg tcactcaatg gggaacttaa
     9421 catttgcaca atatatgatg acatatggta acgacaagtc actgccacag ttaaacaaac
     9481 aagtgaatat tgcagggaca tttaatggtg ttttaaatat gaatgaagat gtcaatgaaa
     9541 ttacagttga taaagatggt aaaccaagtc gaatgaatca accttatcag caattacgcg
     9601 tgctaaaaga tatttataaa ggtaaaggta tcgaagtgct aaatatttat ggggatttga
     9661 aagatggtac acattcagac ggcagagtgt ctaatagttc ttcgaaatca ttaaaatatt
     9721 tactgggaaa tagtccgaaa agttatcgag aatctaaata tgaaggtgag cctgcacaac
     9781 atagtcagct tcatgagaat gaaaatgttg ctaatgagtt aatcgacttc ttatggaaaa
     9841 aataaagcaa ctgctagttt ttctggggca aaaaagcttg taatattaga gcttttcaaa
     9901 aattatctat tgtttgagag tttccataaa atgtctaagt cgtaacattt taaatttatg
     9961 attatagcaa atataatgtg gttgaagtta catgaatgaa tcagtgctta agtcaaaata
    10021 taacaactaa gaatgtattt gcatacagac atcacgaaga cacaattcaa acttaacgct
    10081 taagtaacaa ataagtaaaa gtttcaaata aagaaaggtg attaatgatg caaaataaac
    10141 aaattctttt taacaaaata ccagaaggca tgcctcaaga ggatacattc aaaattgaag
    10201 agattgacac gcctaaactt gagagcgatg gtgtacttgt acaaacgctg tatatttcag
    10261 tggatccata tatgagagga cgtatgacta aggcagattc gtatgtacag ccttttgaaa
    10321 ttggtaaacc gattgttagc catgtcgttg caaaagtaat tgattcgaca ctcgcagatt
    10381 ataaaaaagg agatgtcgtt gtaggtatgt tgccttggcg aatcattaat catgtccaag
    10441 cggatcaaat tactaaagtt ccaacgacag atgtaccact tgatttatat ttaagtgttt
    10501 taggaatgcc aggacaaaca gcatatcatg gtctgcttga tattggacaa cctaaagccg
    10561 gtgatactgt tgtggtttca gctgcatctg gtgctgtagg ttcagttgta ggtcaaattg
    10621 ctaagattaa aggttgccgt gtagtaggta ttgcgggtgg cgacaaaaaa gtaaattatt
    10681 taacagagac actgggcttt gacgctggta ttgattataa gaaagaggat tttgcagaag
    10741 cgttagcaca agcagtacca gatggtattg atgtttattt tgaaaatgtt ggtggaacag
    10801 ttggcgatga agtatttaag catttaaatc gttttgcacg tgtaccagta tgtggcgcaa
    10861 tttcgtcata taatcatcct gaagccgata tcggaccacg tattcaaggt acgcttatta
    10921 agaagcaagt aatgatgaga ggcttcttgg taagtgaatt cgctaatgct tttaaagaag
    10981 ctagtgaaca gcttgcaaca tgggttcaag aaggtaaaat tcagtcacaa gtaaccattg
    11041 aagatggttt tgaaaatgcg ccacatgcat ttaaaaattt atttactggc gataactttg
    11101 gtaaacaagt tattaaagta acggaataat aaagtatacg ttttaacaaa ctaactttgt
    11161 taattgacca aaatttaata attttttatc taaagtaaat ttgttcagca acatggtaga
    11221 aataagtcta aaatttaata cgaaagtcgt ctgtaattat ttatatattc aaatgaaaaa
    11281 ggagtcatga tgctgcgata taagcacaca tggcttctat tttgtataat aagaaataag
    11341 ttcataaatg aggtgaaacg atatgaaaat gataggattt gagaagccct ttaaattgga
    11401 agaaggaaat ttatttaaag tatacgaaca aagaaagcca acgccagaaa acgatgatat
    11461 cttagtcaaa gtaaatagca ttagtgtaaa tcctgtagat acaaagcaac gacaaatgga
    11521 agtcactcaa gcacctcgtg tacttgggtt tgatgctatt ggtactgttg aagctatcgg
    11581 accagacgtt acattattta gtccgggtga cgttgtattt tatgctggat cgccaaatag
    11641 acaaggatca aatgcgacat atcaattggt gtcagaagct atagttgcaa aagcaccaca
    11701 caacataagt gctaatgaag cggttagtct accgctaact ggtattacgg catatgaaac
    11761 gttttttgat acatttaaaa tttctacgaa tcctgctgaa aacgaaggga agtctgtctt
    11821 aatcattaat ggtgccggtg gtgtaggcag tatagccact caaattgcta aacggtatgg
    11881 tttaactgtc attacaacag catcacgaca agaaacaaca gagtggtgtg aaaagatggg
    11941 cgcagatatc gtattgaatc ataaagagga tttagtgcgt caatttaaag aaattccatt
    12001 agtagattat atattttgca cctataatac agacttgtat tacaatacta tgattgaact
    12061 gataaaacca ttagggcata ttacgactat cgtagctttt aatgaagatc aagatttaaa
    12121 tgcattaaaa ttaaaaagta taacatttac acatgaattt atgtttgcgc gtccaattca
    12181 tcgaacgcct gatatgatta aacaacatga gtatttagaa gatattacga aaaatataga
    12241 atcagggcat taccaaccaa caacgacaca agtttttgaa ggtttatcac ctgaaaactt
    12301 atatcaagca catcaattgt tggaaaaaca atcaatgatt ggaaaattag taattaatat
    12361 atagattagt aagtaaagtc gccttatgca gaaaatactg tataggcgac ttattttttg
    12421 tgtctagcga ctattgattc taaacggaaa tatggcaact ttgcaggtat caaatcaata
    12481 atgcactaga aaggcattac ttaaatacaa taaaatactt tgttgctcaa tgataaataa
    12541 tttgagtgtg ttttatatat catttttaaa agatgaggca ttattaattg tgtctatata
    12601 aaattacaaa tgattaaaat gttgcagtta tgggtatgaa cttaccatta cacagaaagg
    12661 gtttgtattt atgaaaaata aatcagtaag tctagtgttt tgggtatctt tagtgatatg
    12721 tacgatattt gttgcttttg gagcaatatt tccgaaacag ttagaaaaac tcacgcaaaa
    12781 cattactaca tttattgcgc tacatttttc ttggtattat ttattgctcg ttttagttat
    12841 tttatttgtc tgtgtgtata ttttattttc gagatacgca aacattacat taggtgaaga
    12901 aggcgaagat cctgaattct cattgccgtc ttggtttgct atgttattta gtgctggtat
    12961 gggaatagga cttgtgttct ggacaacggc agaaccaatc agtcatgcct tcaaattaac
    13021 gcctattcat aaagctggca cgcaatctgc aattaatgat gcaatgcagt tttcattttt
    13081 ccattggggg attcatgcat gggcagtcta tggtattgtc gcgctagtgt ttgcatattt
    13141 tagctttcat aaaggatatc ctggcttagt cagcgctaca ttgacgcctt tattaggtga
    13201 aaaggcaatg cgtggacctt taggtggtgc cattgatgta ttggcagtca tagctacagt
    13261 aacaggcgtt gctgcaacat taggtttcgg tgcattgcaa ataaacgaag gtttacattt
    13321 tttatttaat gtaccgagta acttcacgat gcaagtaatc ttgattgtca ttgcaaccat
    13381 attatttact tggtcagcat ggtcaggtat cgataaaggt atcaaaacgt tgagtaatat
    13441 caacatgtta cttgcttttg tagtacttat tggtcttttt attgttgggc caacgctata
    13501 cattttaaat acatttacga atggtctagg taattatatt gcaaatttct ttagtatgag
    13561 tttacgcatt ccgacaggtg gtcaaaaatt ccaatggtta caaaattgga caatctttta
    13621 ctgggcatgg tggatatctt gggcaccatt tgtaggtata tttattgcac gtgtgtcaaa
    13681 aggacgtacg attaaagaat ttattctagg tgtacttttt gtcccggcgt tagtttgctt
    13741 tatattcttt gcggtattcg gcgcgtcagc aatttactta caagataatc atattgccga
    13801 tattgctaaa gctgcgacag aaacagcaac ttttgcgacg ctacaacatt atccgctagg
    13861 ttttgtatta agcttaatta ctttacttgt tattatgatt ttctttgtta catcagcgga
    13921 ttcggcaact tatgttttag gtatgttgag ttcaagtggt gatattaatc cgaagtcatt
    13981 tgtcaaagtg agttggggta tcattatggc attatttgca atcatcatga tttacacagg
    14041 tggtactcaa gcaattcaaa atttattaat cattgctgcg ttaccatttt ccgtggtcat
    14101 tatagcgatg atatggtcgc ttataaaatc tttgagtgag gagaaaccga gaaatagtaa
    14161 taaggtttta attaaacatc gtgacccaga tattttagaa tatcgttctt caaaacatat
    14221 tgacgaaaat taattaataa tgaggtttta tttacaaaaa ctagggaata tattactaaa
    14281 tggtacttca tcttatatat taaaaataaa tttaaagtta ttaatttatt atgtttaaga
    14341 aaaaaactga tgggtacata tttaatatgt ttttaaatta actttaacta aggaggtgtc
    14401 gttaggggtg aaacgactta aaaactttat ccttggctta ttaatagtag taatcgtggg
    14461 gttcctgtta ttcatgtata tccaagatgg acgtataact gaatatcaag actacttcct
    14521 tcaatttgaa tggttccaac cattacttat ttcacttgct gcactattga tattaatagg
    14581 tcttatatta gtatttagta tcttcaaacc tacgcatcga aaacctggat tatacaaaga
    14641 cttcgatgat ggtcatgttt acgtatctcg taaagctgtt gaaaagacgg cattcgatac
    14701 ggttgcaaaa tatgatcaag taagacaacc aaatgtagtt gcgaaactat ataacaaaaa
    14761 aaataaatct tatatcgaca ttaaaactga ctttttcgta ccaaataatg ttcaagttca
    14821 atctttaact gaagcaattc gttcggatat taaaaagaat gtagaatatt tcacagaaat
    14881 gccagtacgt aagttagaag ttaatgtgag agatcagaaa acttctggtc cacgagtgtt
    14941 gtaagggagg tatatcatgg ctaacaatca taaccaaaac ggacaagact ctacacaaca
    15001 agtgattaat tttttaaaag tatttaaatg gagaatcgtt ggtttcctag cgtttctatt
    15061 aatcgcgata ttattcttaa cgttaggttt ttggaaaaca gtacttatca tcgttttatg
    15121 tttaattggt gtaggtattg ggtatatgaa agaccgtaag caagatttta tgaatttttt
    15181 aaatcgatgg agttaattca tcaatttaga atttaattaa aaaaatataa aaattaacaa
    15241 gtaaaaggag aatctattat gactgtagat aacaataaag caaaacaagc atacgacaat
    15301 caaactggtg ttaacgaaaa agaaagagaa gagcgtcaaa aacaacaaga acaaaatcag
    15361 gagcctcaat tcaaaaacaa attaacattc tctgatgaag ttgttgaaaa aattgctggt
    15421 atcgctgcac gtgaagttaa aggtatctta gatatgaaag gtggcttaac tgatacattc
    15481 actaatgcat tctcaagtgg caacaatgtt actcaaggtg tatctgttga agttggtgaa
    15541 aaacaagctg ctgtagactt aaaagtaatc ttagaatatg gtgaatcagc accaaaaatc
    15601 ttccgtaaag taactgaatt agttaaagaa caagttaaat atattactgg tttagatgta
    15661 gttgaagtta acatgcaagt tgacgatgta atgactcaaa aagagtggaa acaaaaacac
    15721 gaaaaaaata acgaaaacaa taaccaagaa agacaaggtt tacaataagt tattcgataa
    15781 aaagggcttg ggacattaa
//
LOCUS       DALAAR010000058        15399 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000058 DALAAR010000000
VERSION     DALAAR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.060"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..106)
                     /locus_tag="O2458_001967"
     CDS             complement(<1..106)
                     /locus_tag="O2458_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ3887828.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYY"
     gene            complement(383..535)
                     /locus_tag="O2458_001968"
                     /pseudo
     CDS             complement(383..535)
                     /locus_tag="O2458_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(535..1026)
                     /locus_tag="O2458_001969"
     CDS             complement(535..1026)
                     /locus_tag="O2458_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ3887829.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            complement(1210..2268)
                     /locus_tag="O2458_001970"
     CDS             complement(1210..2268)
                     /locus_tag="O2458_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCZ3887830.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(2255..2461)
                     /locus_tag="O2458_001971"
     CDS             complement(2255..2461)
                     /locus_tag="O2458_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887831.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(2686..3030)
                     /locus_tag="O2458_001972"
     CDS             complement(2686..3030)
                     /locus_tag="O2458_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ3887832.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(3701..6073)
                     /locus_tag="O2458_001973"
     CDS             complement(3701..6073)
                     /locus_tag="O2458_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ3887833.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(6165..6413)
                     /locus_tag="O2458_001974"
     CDS             complement(6165..6413)
                     /locus_tag="O2458_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCZ3887834.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(6414..6797)
                     /locus_tag="O2458_001975"
     CDS             complement(6414..6797)
                     /locus_tag="O2458_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ3887835.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(6799..7002)
                     /locus_tag="O2458_001976"
     CDS             complement(6799..7002)
                     /locus_tag="O2458_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ3887836.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(6969..7142)
                     /locus_tag="O2458_001977"
     CDS             complement(6969..7142)
                     /locus_tag="O2458_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ3887837.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(7154..7426)
                     /locus_tag="O2458_001978"
     CDS             complement(7154..7426)
                     /locus_tag="O2458_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCZ3887838.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(7419..7682)
                     /locus_tag="O2458_001979"
     CDS             complement(7419..7682)
                     /locus_tag="O2458_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887839.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            7875..8207
                     /locus_tag="O2458_001980"
     CDS             7875..8207
                     /locus_tag="O2458_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3887840.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            8219..8677
                     /locus_tag="O2458_001981"
     CDS             8219..8677
                     /locus_tag="O2458_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ3887841.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            8694..9421
                     /gene="seq"
                     /locus_tag="O2458_001982"
                     /pseudo
     CDS             8694..9421
                     /gene="seq"
                     /locus_tag="O2458_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
     gene            9445..10173
                     /gene="sek"
                     /locus_tag="O2458_001983"
     CDS             9445..10173
                     /gene="sek"
                     /locus_tag="O2458_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCZ3887842.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIY
                     GGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEY
                     NIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKI
                     YEDNKTVESEKFHLDVDISYKETI"
     gene            10261..11481
                     /locus_tag="O2458_001984"
     CDS             10261..11481
                     /locus_tag="O2458_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ3887843.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(11624..12445)
                     /locus_tag="O2458_001985"
     CDS             complement(11624..12445)
                     /locus_tag="O2458_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ3887844.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(12463..13158)
                     /locus_tag="O2458_001986"
     CDS             complement(12463..13158)
                     /locus_tag="O2458_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887845.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(13151..14176)
                     /locus_tag="O2458_001987"
     CDS             complement(13151..14176)
                     /locus_tag="O2458_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887846.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(14265..14368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(14426..14722)
                     /locus_tag="O2458_001988"
     CDS             complement(14426..14722)
                     /locus_tag="O2458_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ3887847.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(14715..15101)
                     /locus_tag="O2458_001989"
     CDS             complement(14715..15101)
                     /locus_tag="O2458_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCZ3887848.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
BASE COUNT         5047 a         2680 c         2086 g         5586 t
ORIGIN      
        1 tataataatt ttcattttct aaatctcttt tagcactact cggtacttcc attcctcttc
       61 ttcttagaat tttcaattgt ttattatgac tttcaaatgg cttcataaat ttacccctat
      121 aaaagtagcc ataacccgaa tagagttatg gctagatcgt tatatatata atacatttaa
      181 ttttatcggt tgtaaataaa taagaaataa caaaaagaga tattttacac aattacctcc
      241 tttattttat tgctactcct caaacccacc aatattatca ataaacactg gtgttgttac
      301 atttaagtct actttctcag taaataagct atggtatcaa actaattagt attaatttat
      361 aaatagcata gcttcatttt cttcaatcct ctaacggtat atcatccaca atcacagtat
      421 gattatggtt agcgttagat acatcttgca ctgccttatc taattcctca tcatctccgt
      481 cccattcacc aatattaatg aatataggaa tattcccgtt aatatcatgc ttatctatga
      541 aagttctttt ttaacttgtt ctaaatgctt attaaactct attcgatgta attcttctcg
      601 ctggttttgt cgttcttgac gctttctact ttcaacttta taaatatcta cttttaaacg
      661 ttcaatgact ccttcatttt taactttccc actacttaac atgtacctca taacatcctg
      721 ttgttcattt tcagaatagt ttttgatgat atttttaaac aaatttagcc tatcctgagc
      781 tgttttcatg taatattcaa gtttatcttt ctcttctata atataaagta ctaatttatc
      841 taatggatat gatacagata ccagcatatc gttatcattc gtgatcacat ggttcatgtt
      901 caagtgatat aagctttcta tttgtgatct catcgcttta attttgctat taatgacttt
      961 cggattgtaa tgcattaaaa gttctacttc agtgatttta tcattgcttt caaagtcaga
     1021 taccaaataa ccacaccttt acatatcgaa tttaatattt ataagtatct gaaaaaggga
     1081 taaatcaagt tatccccctt tcataaaagt ttatccgcgt tgcaagcgaa gggcccccgc
     1141 cggtacccgg cgaaaaaaca ttttaagccg atgggcaggg ggctataaaa ttttatttaa
     1201 ataatttttt tatttaatat taactatgcg caataactct ggaattattt ttatatcaaa
     1261 cacatagttc aatgcaaatt gataaatacc ttttaatgct tgtttctttt tgttatacat
     1321 aataggttcg tatttagcat atatggcatg tccaaagtct ccaacaaaga catcatgtgc
     1381 atcatcagta accactacat tagtcacatt aaataggtta cttaaatcac ctgcaagtcc
     1441 acctaatccc atctgtgcca actctttgat gagcttatca tgatgttctg tgttcatcac
     1501 aatagataca ttacgcctaa acgtttgtgg tatatctttc atcgcttgct tgataccatc
     1561 gtatattgat gtgttactta cttctttgat accttgatta taaagtgata agtcttggtt
     1621 acctttatca attaaaccac ttgaattatt cttatctaat aacaatgttg tttcatatgc
     1681 gacacactca ttaataaata tctctttaag tagatcatct aatgttctat cagagccatg
     1741 aactacactg tctgatactt ctgtgtagat atgtccctca cgtctatcaa agtatttact
     1801 actgccatta agtgatagct caataccgtt agtagctaac ttatctttaa tgtatgttag
     1861 tgatggtata gattcaacat gttcatcatc agtaatggta atcacctcac gtagtgattg
     1921 aggtagctca caattcacca ataactcttt aaatacttct ttacgtgcag ttggtaactc
     1981 ttgaatatta aagttattct cactactaac taattgtttt gttaaggtac ctacccatgt
     2041 attacttgat tgatttactt cattcatatt taacactcct aataattttt tgttaatttg
     2101 ctctggtaat aaaacttctg tttcagtaat acttaattgc tttaacccct gttcaatcat
     2161 acttacatct ttacctatat ccttttgata tagattggca tatcctttaa tatccatatc
     2221 atcaaagtta gatagttgat acccaaattg ttctttaaac tctttcataa tcttcatctc
     2281 ctaatttgtt tttagtatga acaactaaac catatatacg ttcgttgttt ttatcttctt
     2341 catagaacga aatgttatga agaaagtatt tgtctaataa ttcgtgatac ttatttaata
     2401 gttcttgctt atctctatac ttaaacacac gttttacaga ttcatttgta tgatctagca
     2461 cgtcttgcct ctctcctttt aatctgatag atatacattt taataagttc aaaatcagca
     2521 cgcttttttg tatagcgcct tacaacgcga tttatatatt tcgtattcat taattgaacg
     2581 ttcacaggtg tatgtttgat tatttgatat gcgattcttt tatgattaat aaatatacac
     2641 actcccatat ctatacttga tattttaaat gcttatctgc atgttttaat ttactaaact
     2701 aaatgttact ggtagccaat gatctatttt tatattttta ttctttacaa ttggtagcct
     2761 tagaccttta ccatcaataa gatagtacat tggcggacaa atttgcattt caattagccc
     2821 gtcgcgtttt aaatctgcca caatgttaaa cgcttcttca ttccaaccca tccaaaatat
     2881 aatattctta tggttgccac tggtatatgc gccgttacct ttgtatttaa aaccttgttc
     2941 ttcaaataca ttctcaatct ctacaaatga agtgctgtca ttgttcttta tatattctaa
     3001 gatgatttgt ttcattctat ttctattcat gttactctag tcctccaatc tatccagtta
     3061 ctaaagcaac cacgttagat actaaaaacc actgaattta tgtttttcaa taaccactgt
     3121 aaacacagtg atgtcaatac atacaggctg ttggttactg agttactgaa aattcacggt
     3181 attttatata atcttttatt accttaattc tctattatac atttctacta aaaagaaata
     3241 atttatagta actcagtaac tttttgtcaa aaagactaat atgaaagtaa aaactgtggt
     3301 tactgaattc tagaatttta gtatctttta gtgacttaaa cgttttttag taactttagt
     3361 atcttaggca attttgtata actgcaagtc tcttccgatt atgtgcaact gaaatttaca
     3421 gctatgcaac tcgttttaaa ttttccagtt gcaatttttt tcttactgtc acaaggggtt
     3481 aatccacttt tgcaactatg caaccgaaat cacacaaatt agtttatata atttttgata
     3541 ctatttctta tttatatttt ggttatttac ttttctacta ataagttata gattttgagt
     3601 tgctcagttg cataagtgcc ctaaaccctt gatataactg tgattatagg tgcaacccgt
     3661 tttaaaattt tcggttgcat tcggttgcat tcggttgcaa ctatattgct tttagcttca
     3721 ccttttcata cgatttatag ttttttccac tttcaatagg ttcaatgtaa tttctattgt
     3781 cattaaagtt gtatatcctc ttagcaagtt cttcattgtc atatcttcgc tgtgcatcag
     3841 ttttccaata attctctaaa taatgttcaa attgtttata aaacttgttt gaactcaatg
     3901 cattatagcc attttcatca caatattctt tataaaatgc gtatacaata tatttcggca
     3961 cttttctaat agtccattga tcaaacatat ttactttaaa cccataaact ggatcgttat
     4021 cttctttaaa tacttcaagc atttttttag atgcatcagg aatgtcaaag gtttcaaaat
     4081 ctaaattaat tgctttataa agcacatact ctagcacttg ctgatttttt atatagtctt
     4141 ctttgatttt aaaattctct ttaatgccat taaaattggc attaaacggt actatcaata
     4201 accttcttaa agtcccgcct gttttgtctt taaatttagg cattccattc gttgattgaa
     4261 taactgtaca cttaaaagtc gctctatata agggtttatt tttaaactca accaacactg
     4321 gatcgccagt aactacactt ttaaagtttg aagaatcatc gacatacaca ccaactggta
     4381 catcgtctcc aattactgct gtcttgccct ctaacacact caatttaaaa cgttcatcaa
     4441 actcattcac ttttaagcta gcaatattgc tataacctat tacattagac aataattctt
     4501 gaaacgtccc tttaccatta tttccatcac caacaaagaa aatagccttt ttacgagtgt
     4561 agttaccatt cattgagtca ttaattactt gccacaataa tttaacaacc tgattatcat
     4621 tacaagcaat ctcttctatc caccgatcta tattccagcc atttatttca ggtactatat
     4681 cttgccttac atacgatgta tctattttac tggtaaatat ataatcaggt gtaaatgatt
     4741 ctagttgctt cgttttacgg ttaaatacac catttttaac tggtattaag tatggtgagt
     4801 tagttttctc ttttatatct accatattgg ttaaatgata aataacttca tcagctttgt
     4861 tgctattatg tttgggctct aaataggaaa tcactcgttt tataattgta gtgttctgtg
     4921 tatatatccc ttcatcaaat tgatacatag ctaacttagt attttcttca tcatcaaaaa
     4981 gtataaatgt taaatgctca ttcaaaatat atgcacattt tatagggcta attgttgtag
     5041 gctttcgccc tcgttttcct tcttcaatcc aattttcttc catttttgtt ctttcttcat
     5101 gccccaattg attcaacaac tgtttaacat ctttactccc ctgtggtata acattaaatt
     5161 catctatcga tttagtataa attgtctttt gattctttat agctttatct aactcaagct
     5221 gtccccaagt cgtattacct cgtttacttt cccatttatc cgtaagatta ttgtagttta
     5281 aaaatatacg ctccatttgc tgtttgtttt tacctgtgta aaacgctaag tagtgtaata
     5341 agctttgtac tgcttcgctt gaactctcaa aatatgattc atatgtgcct tgtaatagat
     5401 ctttaatttt atctttttgt ttagatttca tcataatgtt tataatatct tcatcagata
     5461 attgtatatt ggtattagat tcctctctca caacctcatt tgctggcaaa ttctctttaa
     5521 aatattcatc aactagagta tttaatactt cttgattatc acaaatatca ctttgaccaa
     5581 ttgtgcatcc tgtaacagtc ataaaccttg ctgaatcata taactctatg tctaaatctg
     5641 ttcttttctt tttacggtta tctggtagtt tacctttaaa gaaacaatgt aatcctgtac
     5701 cacttggaga cttttcacaa tatgtgagtt gcatcatttt tagtgctaat tcagaattga
     5761 tttgtccttt tttatcaatt gcattatcaa tatctaggca aacaaaatta ttaccatcac
     5821 ttaatacaaa acctataccg ctatattgct catcaacttc atattcaata cttacacttt
     5881 caaagtcaca ccatgttttt ttattggttg tactagctct gtaaccatta atactatatg
     5941 gtacttttcc aaaattttgt tgcttctcgt tccattcagc tcgccataat acccaattgg
     6001 gcactaattt taattcttgc ggtatttcta attctttaac tccaataatt ctatcttttt
     6061 tcttaattgc cacagaatgc ctcctttcta atctgatttc tctgtatttt caatgcaact
     6121 aatgttaaaa tattaatggt atgattttat aaatttttat ttttttatgc gttatcttgg
     6181 ctttggtcgg cgaaagatga cgctttttct aatgcatcaa acatttgatc attgtgaata
     6241 cgtctctcat gatatgaaaa tccttcttta atagcatcgt ctttatttaa ggtatgattt
     6301 ggctcatgta gatataaatc ttcaaaatgc gagccatgat tatcctttaa atcctcaaac
     6361 ttttctttca atatctttaa atcacacatt aaatttttaa tttcccaatt catttataat
     6421 tcctccactt caatatttcc cacaatgtaa tctaatgccc actctaacat tccaatcacg
     6481 tgtcctttgc gatctgttgt atgtttatgt tcaccttttt cgtctatgac actataacgg
     6541 taaacgtgtt gtgtttcttt catgacatca cttagcgtca ttgttacttc atcaagaatt
     6601 aaaaatgctt catcttcaaa atctaattca gcaagaatat tgaacaatcc attatgaact
     6661 aactttaaag catattcata taatgcttta tcctgataat gatagtcctt atcggattga
     6721 aacttatctt cctcgtataa tactacttct aatattttaa ccacttttga taattgatat
     6781 ttttgtttga tttccatctt aactacctac ttttttctta tttttaattt ctgtaatttt
     6841 tgataaatcc atttctaaac acgcaacttg aacatcttta ctaacatcag gaaagtactg
     6901 ttcaaatact tttggcggta tatttaacat taaattatgt tgagtatctt taatgttgta
     6961 ccagccaact actgtttttg taataataac ttgttttcgc acgttgtaat ctctcctgtt
     7021 aaattaaatc cataagttac catcatgccg tacacactaa aagcgacata catgttagat
     7081 attgctagta ataatattgt taacaatgaa actaagcaga tataagttaa gtacattttc
     7141 attgccttgc ctcctacatc cattttttat gacgcgcctt catgtactcc tcgaatcgcg
     7201 gaatactgat aacaatcatt gttgatgata acgagtaata taaatcatca acacctttag
     7261 aatctttttc ccactctttt aaaatgcgat tcaccgaact atatgaaatt cccaaaatac
     7321 tagctagtgc attaggtttc gcaaacaaag gattcactac aacttgcttt ggttctgtaa
     7381 ttgtattttc tttagctggt acatcatgca attttgttct agacatttaa ttcacctcct
     7441 tttaagatgt tttttcgttc ttttccggga acgttattgg taaaaaaaat gtctaggtta
     7501 tttgtttcat aacctaatat ttttgccatt ttaataaatt cattcgctcc aatatctact
     7561 attccatttt ctctctttgc ataaggcgtt cttgttttcc accccatttt gtgagccatt
     7621 tcatcttgcg taataccaca agctattctt tccgctctca atctttttaa atttagtacc
     7681 acgtttacac ctcctatcgt tctcgttcga gaactaatta caatttacca tttacgttct
     7741 cgttcgtcaa caatttttac ttaaaaaaat tcaaaaagtt ttttcttcct tatatattgt
     7801 attcttttgg gaacggtgtt ataatcaaat cgttcacaaa taagaacaaa tattcaattc
     7861 aggagataca agaaatgaga actaatgatg aaataatcac aataattaaa acatcaatga
     7921 aagaacaaaa tatgtcacta agtgaattag ctcgtcgtgt aggtgtagca aaatcagcag
     7981 tatcaagata tttaaattta actagagagt tcccattgaa tcgtgctgaa gattttgcga
     8041 aagtacttgg aataaaaaca gaatatttat taggatttgc tgaacgcgaa gaatctacaa
     8101 aacaagatac tatcgccgcg cacttagatg gagattttac agaggaagaa ttaattgaaa
     8161 ttagaaagta tgcggagtta gttagaaaag cacatcgaaa tcagtaaggg gttattgcat
     8221 gtatttatac gaaaagatgg ttattgaaaa caaagagata ccaattgatg atgggaaatc
     8281 tttaggtaat tttgagggtc tctatgacaa tggggttatt ttaattaata aaaatttgtc
     8341 agaaacacgt aaagcagaag tgttatatga ggaacttgcc caccacaagt tgacgtatgg
     8401 caacatttta gaccaatcaa aatggattaa tagaaaattt gaaaattatg cacgtagaca
     8461 cggctttatc tcagctgttc cattacgtga aattgtagaa gcttataatt atggcgtacg
     8521 taacttgtat gagttgtccg agtacctaca actgagtgaa aaatacatat tagaagcgat
     8581 agaacaatat aaaaagatat acggtattgg tacccactac ggcgagtatt caattacatt
     8641 tgagccgttg agagttttta aatataaaga aatataacaa aggagaaatg aaaatgaata
     8701 aaatatttag agtactcact gttagcttgt ttttcttcac atttttaata aaaaacaatc
     8761 tagcatatgc tgatgtaggg gtaatcaacc ttagaaactt ttatactaat tatcaaccag
     8821 aaacgcttca aggagttagt tctggaaatt tttctacttc tcatcaatta gagtatattg
     8881 atggaaaata cactttatat tcacagtttc ataatgaata tgaagcgaag agattaaaag
     8941 atcataaagt agatatcttt ggaataagtt actcaggtct ttgtaataca aaatatatgt
     9001 atggcggaat tacgttggcg aatcaaaatt tagataaacc tagaaatata cctattaatc
     9061 tctgggtcaa tggtaagcaa aacactatat ctacagacga ggtttctact caaaaaaaga
     9121 ggtaactgct caagagattg atattaagtt acgaaaatac ctacaaaacg aatacaatat
     9181 atatggtttt aataaaacaa aaaaaggtca ggaatatgga tatcagtcaa aatttaattc
     9241 tggatttaac aaaggaaaaa ttactttcca tttaaataat gaaacttctt ttacatacga
     9301 tttgttttac accggaactg gtcaagcaga gagtttttta aaaatttaca atgataataa
     9361 aactatagat gcagagaatt ttcatttgga tgtagagatt tcatatgaga agactgaata
     9421 aatagaattt aaggagaaaa tgcaatgaaa aaattaatag ttatcatatt aataaatata
     9481 ataactttaa gtgtctctaa tagtgccagc gctcaaggtg atataggaat tgataatctc
     9541 aggaattttt atacaaaaaa agactttgta gatttaaaag atgtaaaaga caatgatact
     9601 cctatagcta atcaactaca attttcaaat gtatcttatg atttaatttc agaatcaaaa
     9661 gattttaata aatttagtaa tttcaaggga aaaaaacttg atgtttttgg tattagttat
     9721 aatggccagt gtaacaataa atacatatat ggtggagtca cagctactaa cgaatatcta
     9781 gataaaccta gaaatatccc tataaatata tggatcaatg gaaatcacaa aactatttct
     9841 accaataaag tttcgacaaa taaaaaattt gttaccgctc aagagattga tgtcaaatta
     9901 agaaagtacc ttcaagaaga atacaacatt tatggacata acggcactaa aaaaggagaa
     9961 gaatatggtc ataaatcaaa attttattct gggtttaata ttggtaaagt aacgttccat
    10021 ttaaataata atgacacttt ttcatatgat ttattctaca caggagatga tgggttacca
    10081 aaaagttttt taaaaattta cgaagacaat aaaactgtag agtctgagaa attccatttg
    10141 gatgtcgata tttcttataa agaaacgata taaatctatt aattatgtca taatcattat
    10201 tttccgggta gcatgcctac ccttattatt ttttgccaat tttgaggagg gagaagcaaa
    10261 atgtggttag aaaaatttaa aaataagaat aaagaaacta aatatagata ttacgagaag
    10321 tacaaagacc ctctcactgc caaatggaga cgtgttagcg tggtacttaa taagaatggt
    10381 aagcaatcac aaaaagaagc tcaaagactc ttaaatgagc gtatagaaga aaaactgaat
    10441 gataagaaac ctactacact taagtcacta actttccatg tcgcatgtga tgagtggttt
    10501 cagaattata tcaaaacgtc tgggtcaaaa agaacaacta ttaaaactaa attgagtaag
    10561 ctaaacactt tgaagaaatt tgtagatgaa gatattttaa tcaataaaat aacactttca
    10621 tatgcgcaac aagttttcga tgaaatggat agtaaaggtt atgtatatca agtaaacaaa
    10681 gatgcgttaa gcatattcaa aaatgtattt gaatacacta gacgtatcta taaactgcaa
    10741 gatttagaat ttttaaaaga tataacatta aataaaagaa ttaagtctta tgatgaagtg
    10801 aaagctaaac gtaataagta cctcgaatta aatgaaatag aatctatcat caaagatatt
    10861 aatacgaagg ctcagaagat gcactcaggt atccataagc ggttttattt attcgttgca
    10921 ctcatgacag aattccaagc cttaaatggt atgcgtattg gtgaaatgtt agccattcaa
    10981 aatgaagata tagactttga taataagagt ttaaatatta acggaacaat ccattggttt
    11041 cacgatgaat ctggtggatt cggtgtaaaa gataccacta aaacagagtc tagttataga
    11101 acaattggat tgagtagcag aagttgcgag atattaaaga aagcaatact ggagaacaaa
    11161 aaggattcaa aatggaatga tggatatcta aatagaaatt ttgtatttac taatcataaa
    11221 ggcaacccaa tgcagactga aagattcaat aaaatcctta gggaagcagc taaagatgtt
    11281 ggtattgata aagaagtttc ttcacatata ttaagacata gccacatatc attactctct
    11341 caacaaggcg tgtcacttaa agctattatg gatcgtgtag gccattccga ccaccgtaca
    11401 acactttcca tttattctca cgttactgaa cagatggata aggatatgat gaacaaactt
    11461 gaacaggtaa aacttggata atgcgctcaa atctgagcgt atttttttgt ttaaaattcg
    11521 ataatttgca tatactgccc cttttgtgcc cttttttatt ttcaatacca caaaaataac
    11581 cccttaatcg ttgttattaa agggtatttt tatttaattg gtattattta gcaggaataa
    11641 ctgcaccatt gtatttttca ttaatgaagt cttgaatatc tttagattgt aatacttcaa
    11701 ttaatgcttt gattttctta tcatcttgat gtccttcttt aacagcaatt aagtttgcat
    11761 aaggattatc tttcgcactt tctacagcaa tagaatcttt tttaggattt agtttttgtt
    11821 cgattgcaaa gttcgaatta atgataacag catcagcgtc ttcattttga taaattttag
    11881 gtaagaattc tgctgattgt ttattattaa acttaatatc ttttttattc tctgtaatat
    11941 cactaaactt agcatcttca atttttacgc cttttttgat tttaattaaa cctgcatcaa
    12001 cgaagaattt taagaaacgt ccttgttcag ctggattatt agacacatag actgttgcac
    12061 cttttggtaa ttcttttaaa cttttatact ttttagagta tacagccata ggttctaagt
    12121 gaacatcacc ggcacttacg attttgtaac ctttatcctt tttctctgtg tttaaatatg
    12181 gtgtatgttg gaaatagttt gcgtcaattt cacctttgtc tagtaattta ttaggtgtag
    12241 tgtaatcgtt aattgtttta atatctagtt cataaccttt tttctctaat aatggttttg
    12301 ctttttctaa aatttcagca tgtggtgctg gtgaagcacc tactgttatt ttcttgtcgt
    12361 cactgccact tttgtttcca ttaccgcaag ctgctaatac aactgcaaat gttaatacta
    12421 aaataagacc aaataatttt ttcataaaat gaaaccccca atttatcgtt tatcaagttt
    12481 atttgtaagc caatccccaa tgaattggat tataaataca ataattaaaa taaaaactgt
    12541 tgatactaaa atgacatcat tttgatttcg agtgaaacct gttaagtatg ctaaatttcc
    12601 taaaccaccg gcaccaatta cacctgcaac tgctgttgaa ccaactaaag cgattgctgt
    12661 aactgtaatg ccagacacta gcgctggcat agcttcaggt aaaaggactt tacgaattac
    12721 tgtccaagta ttagcgccca ttgaccaagc cgcttcgatg acacctttat caatttcttt
    12781 aaaagcaatt tctacgagcc ttgcataaaa cggtgctgcg ccaatgatca aggctggtaa
    12841 cgcacctgtc ggaccactta tcgttccaag tatcaaactt gtaaatggaa ttaataataa
    12901 aattaaaata ataaatggta tcgctctaaa taagttaaca atgaaagaaa cgatagaata
    12961 aaataacctt gcaccgatag acttaccttt agcagacaag aataataaca cacctaaaat
    13021 aagaccaagt ataaatgcaa atatagttga gacgactgtc atgtatagtg tttcgactat
    13081 tgcagtccaa acttctggcc actgaatatt aggcattgta atcatttcat ttataatttc
    13141 actaaatgat ttacccatgt cttaacacct ccattttaac ttgtcgctca attaactctt
    13201 tttcgaattt tccgaaatct acacttgaaa tatatggaat atgcagaact aaaaagccga
    13261 ctgttccatt ttttgtattt ttaatatttg cttctaaaat attaatttta atatcatagg
    13321 cagttgatag actcgataca ataggctcgg ttgttgttga accagcgaaa actaatctaa
    13381 cgatatatgc atctttttct aatggctcta attctgttaa agatgtttcg aaatcatcat
    13441 ttaaatcgtc tttcacaaat cgttttgtca cagtgtgttg cggattttca aaaacctgtg
    13501 tcaccggtcc ttgttctatc actttaccac tttccataac tgcaacttca tcacaaatac
    13561 gacgaatgac atgcatttca tgcgtaatta gtacaattgt taaattttgt tgttctctaa
    13621 tttttagtag tagatctaaa atttcatctg ttgtttgcgg atcaagtgca cttgttgcct
    13681 catcacaaag caagaccgtt ggatcattag ctaacgctcg tgcaatccca acacgttgct
    13741 tttgtccacc tgataactct gatggataag ccttttctct accttttaaa ccgacgagtt
    13801 cgacaagttc taatgctttt tgcttagctc tccttctagg gacacctgca atttcaagcg
    13861 gaaacataat attttttaac acagtccttg accataacaa attaaaatgt tggaagatca
    13921 tacttacttt ttgtcttttt gctcttaatc catttttgga caattgacct atatggtctc
    13981 catctataat aacttcacct gatgtaggcg cttctaaatg attaaacatt cgaatcaaag
    14041 tactttttcc tgctccagaa aaaccaatga cgccataaat cgatcctgct cgaatcgata
    14101 aattaacgtg atctacagca aggacttctt tatttttagt ccgatattct ttaacaactt
    14161 cttttaattc aatcacgttg attcctccct gtgttgctta ataaaataaa aaatgctttc
    14221 tcaatatcga tagaaaaatt gagaaagcaa tagtagtatt gtttctctca tcttcaaaag
    14281 ttaaaacttt atgtgaattg gcaccatttc tatataagac ggttgccggg cttcgtaggg
    14341 cacatccctc caccactctc gataagagtt tacgcatcat ttaatttgta ttaatcctaa
    14401 caccttagta aaatttcgtc aataactatt ttaaattttc taacaaatca gtcaccgatt
    14461 taaatgcata aattcgtttt acttctttat ctttattcat caacaataaa atcggcgtag
    14521 acatgatttg catatcttta caaaactgag gataaaagtt taaatctatt ttcaataatg
    14581 gtaactgcaa tatttcatta gcaatgtcta acattctttc tgaaacctta caagtaccac
    14641 acgttggtgt ataaccaaag attaaatgtt tgtcttcctc ataaaatgta gttacatctt
    14701 tgatgtctaa tgaattattc atttactaaa actaaccttt cattatttat attcggtaaa
    14761 agaggtgttt ctttcttaca agtaaagcca tgttttgaaa gtacatgcgc caaatattgt
    14821 ttggggcaat tcgcaacttg acagtaagtt ttatcaataa atatatgttc actttcactc
    14881 aaataacgtt taaaccaatt tctaattcga tctccttcgt catcagaatc ggctaataca
    14941 aaaacttgtt tatcatacag tgattctatc atatcatcaa gcttatctat actcattgtt
    15001 ccatgagtac aaataatatt gactggttct gcaataacct gttgcaccct ttttttatca
    15061 gattttcctt caacaattat cactttattt acaatagcca tcatcatcac cctttaaaat
    15121 caataaacat ctgtcactgt atcatttcac aaaattggta tgaataaaac ataaatcaca
    15181 aaaaatttaa actagcttaa tataataatt acaaactcaa tgtttgacta gctggaacat
    15241 ttaacataag cagacaaagg ctaagtcaaa aatcaacatc ctaaaatcta caatgttata
    15301 ttaacaatag ttaaccaaaa gaaaatacac ctataacaaa cttttcaatt atagcggggc
    15361 cccaacacag aagctgatgg taagtcagct tacaataat
//
LOCUS       DALAAR010000059        15295 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000059 DALAAR010000000
VERSION     DALAAR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.061"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(79..777)
                     /locus_tag="O2458_001990"
     CDS             complement(79..777)
                     /locus_tag="O2458_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCZ3887849.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(794..1783)
                     /gene="floA"
                     /locus_tag="O2458_001991"
     CDS             complement(794..1783)
                     /gene="floA"
                     /locus_tag="O2458_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCZ3887850.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1801..2484)
                     /locus_tag="O2458_001992"
     CDS             complement(1801..2484)
                     /locus_tag="O2458_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCZ3887851.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(2728..2904)
                     /locus_tag="O2458_001993"
     CDS             complement(2728..2904)
                     /locus_tag="O2458_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCZ3887852.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSFSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(3197..4543)
                     /gene="mtaB"
                     /locus_tag="O2458_001994"
     CDS             complement(3197..4543)
                     /gene="mtaB"
                     /locus_tag="O2458_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCZ3887853.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(4550..5302)
                     /locus_tag="O2458_001995"
     CDS             complement(4550..5302)
                     /locus_tag="O2458_001995"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCZ3887854.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(5304..6242)
                     /gene="prmA"
                     /locus_tag="O2458_001996"
     CDS             complement(5304..6242)
                     /gene="prmA"
                     /locus_tag="O2458_001996"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCZ3887855.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(6246..7385)
                     /gene="dnaJ"
                     /locus_tag="O2458_001997"
     CDS             complement(6246..7385)
                     /gene="dnaJ"
                     /locus_tag="O2458_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCZ3887856.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(7521..9353)
                     /gene="dnaK"
                     /locus_tag="O2458_001998"
     CDS             complement(7521..9353)
                     /gene="dnaK"
                     /locus_tag="O2458_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCZ3887857.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(9422..10048)
                     /gene="grpE"
                     /locus_tag="O2458_001999"
     CDS             complement(9422..10048)
                     /gene="grpE"
                     /locus_tag="O2458_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCZ3887858.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(10080..11057)
                     /gene="hrcA"
                     /locus_tag="O2458_002000"
     CDS             complement(10080..11057)
                     /gene="hrcA"
                     /locus_tag="O2458_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCZ3887859.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            complement(11157..12281)
                     /locus_tag="O2458_002001"
     CDS             complement(11157..12281)
                     /locus_tag="O2458_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCZ3887860.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            complement(12844..14667)
                     /gene="lepA"
                     /locus_tag="O2458_002002"
     CDS             complement(12844..14667)
                     /gene="lepA"
                     /locus_tag="O2458_002002"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCZ3887861.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            15013..15264
                     /locus_tag="O2458_002003"
     CDS             15013..15264
                     /locus_tag="O2458_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCZ3887862.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
BASE COUNT         4557 a         2939 c         2117 g         5682 t
ORIGIN      
        1 ttctctttgt tttttaattt ttcttaacgt aaaaaaacaa gcaactacca ttacgatagt
       61 gcttgttttc aaaaaatttt ataattgttt tggtttagct aaaatttctg accatatcat
      121 accattgatt acttcatctt tatcaaattg gaacgctgat ttagtcaaat ctttttcgac
      181 attttgagaa ttaagcagct gctttaattt caaacgtttt gtacgttcag ataagtattt
      241 atcctcaata atatctctag cacgtttttc cattttagca atttgctttt ctttttctct
      301 atcaatgtca gtacgaatcg ttttaatatc atctctaagt gatttttcta attgacgtct
      361 aatttcgtca gaattatctt gtctagaagg tctatgtttc tgctccaccg gttctgcttt
      421 aggcactgtg attggtttct ctgtcatcgg ttttgtttct tgttgttttt taggtgccat
      481 aggttctaca acaggtttcg atttaggctc ttcctttgga agttcatcga ataaaggtgg
      541 taacgtatca tcatattttc gtttcgatga tttcttttct tcttcattta attcttcact
      601 tatttcttta aacgttcgct caatttcttc aaaaaagcca cctttttttg gttcattatc
      661 ggtagatgtt ttttgaggtg gcttttgatt ttgtctatct ttatgactat tttcgcgcat
      721 agtagtaatg atagaaatga tcactgatat gacaaaaatt agaataccga cactcattta
      781 atcacctctc gacttaatgt tcaggtgact catcatcact ttgatcagtt cgtttattaa
      841 ttgcatttct catgcctgta tcagcttcga tatttttcaa attataataa tctttaacac
      901 tgatattacc tgaacgtaat gcttcagcca tagctaatgg tacttcagat tcggcttcaa
      961 ctactttagc atgcatttct tgtacacgcg ctttcatttc ttgctcagtt gctacagcca
     1021 tagctctacg ttcttcagct tttgcttgtg caatattttt gtctgctaat gcttgttcag
     1081 tttgtaagtc tgcaccaata tttttactaa tatcaacgtc agcaatatca attgataaaa
     1141 tttcaaatgc agtacctgaa tctaaacctt tgcttaaaac tgttttagaa atattatctg
     1201 ggttttcaag tacttctgta tgatgcttac tagaaccaat tgttgaaacg ataccttcac
     1261 caacacgtgc gatgattgtt tcttcaccag caccaccaac aagtcgagca atattagctc
     1321 taactgtgat acgagctttg gctttcactt caataccgtt cattgctaca cctgcgataa
     1381 atggtgtttc aatgacttta ggattaacag acatttgaac cgcttctaat acgtcacgtc
     1441 ctgcaaggtc aattgcagca gcacgttcga aaggaagatc aatgtcagca cgttgtgcag
     1501 caatattagc gtcaacaact ctgtcaacat ttcctcctgc tagataatgc gattctaatt
     1561 ggtttgttgt taatgctagt cctgctttgt gcgctttaat taatggcgct ataacttttc
     1621 ttggagatac acgacgtaaa cgcataccaa ccaatgtacc tataccaaca tgaacgccag
     1681 ctgctaacgc tgaaatccat aaaccaatgg gtacaaatga gaataaaata agtaatgcaa
     1741 ctactataat aactgctatt acgataaaac ttaaactaaa catggtatcg ctccttttaa
     1801 ttaatctact tccctcacaa ctactcttgt tccttcaact tcaaggattt ttaccgtttt
     1861 attgcgcaaa ataaagttgc catctgaaac agcatcaata cgttcatttt cacaaaaaat
     1921 aatccctgca ggtcgaagat ctgtaactgt ttgagcagtc tttcctacga ggtgcgagcg
     1981 gttatcatga gaattgtaac ctgactcaga attagttgaa tcttttaaga taactttatc
     2041 caaaaacgga atctttctgt tgaaaatctt cactaatatc acccattcta caatcgttaa
     2101 aatcaaggca acgataacat tcgcaagcat aaatagcaaa ttatcaccga gcgttgttat
     2161 gcttatagtt atcagtatca tgccaataat accaattact gcaccaacta caaataattc
     2221 aattacaact aatataacgc caattgagaa tattaagata gaatgcatat tgacatttcc
     2281 ttggattaga aatcccaaaa ataaaataag taatgataat gtggcgataa taccagctgc
     2341 attgattttt ttagagtaaa gttgatatac aaatcctaag aatgtcaaac aggttaatat
     2401 taacgtaaaa ataggttgaa caattatatt gctaacttgt tcaacccaag tatctccagc
     2461 cgttgattcc aagatagttg tcatttgtaa aaaattatta taactcacaa tttcacctcg
     2521 ccctcacatt aattatacat gaaattgaat atactttata gttataagaa cctatagaat
     2581 atcaatttga ttatttataa actatataac tttttttaat taaaaattga caacaaaaaa
     2641 acatcacata ttagtgtcag acactaactt gtgatgtttg ttttatataa ttaatattcg
     2701 tgttgaggga gtcaacagag gtattaatta tttgaattta cgtttacgtg cagcttctga
     2761 tttcttttta cgttttacgc ttggtttttc gtaaaattca cgtttacgta cttcttggat
     2821 tgttccactt ttagaaaatg aacgtttaaa tctacgtaac gcatcttcaa gtgattcatt
     2881 tttacgtact actgttttag acatctgtat ttccctccct ccaaatatca acggaacttt
     2941 atagtcaatt gcacagtaaa actatgcatt tataagtata atatatctgt aatttatggt
     3001 caattagtaa attgtttttt atttgaaaca tatttcacat caaaatcaca aagactttta
     3061 gattttgttc taaaaatctc ttaataattt atttaatgag aagagttgct tatatagtaa
     3121 attgtgaagc cgttaaaaca acgttacaaa acctatatct ttaatacgga accatatggt
     3181 atgaatcaag gaatacttaa actaaaactt ctctatcaga tttatttgtt gcgaaatcaa
     3241 caactttaat tgcttgccct tcatttaatg gataatttgc ttgcgtaatt ttaactttta
     3301 caatttgacc tatgagtgat tcgtcacctt caaattgtac tttcatataa ttatctgcat
     3361 atccaactaa tgtaccttct gtgtcaccct gttcctcagg aattacttca agcacatctt
     3421 gatcaaattt agacgcatat aactttccga gttgattgct tagcgtaatt aacttatgca
     3481 cccgttcatt tttaatttct tcatcaattt ggtcatccat tcttgcagct ggcgtgccaa
     3541 ttctaggaga ataagggaaa acatgcagtt cagagaactt atgctttacg ataaaatcat
     3601 atgtttcttg gaactcagct tcagtttcac ctgggaaacc aacaattaca tcactcgtaa
     3661 ctgccaagtc tggtaaagct ttatgcaatt ttgttaatcg ttctgaaaat ctatccattg
     3721 tatacttacg tctcatacgt tttaatactg tatctgaacc agattgtaat ggaatatgca
     3781 aatgacgcac aacttttgtt gaacgttcta aaacgtcaat tacttcatct gtaagttgac
     3841 ttgcttcaat tgaagaaatt cgaattcgtt ctaatccatt aatcgtttca agatcacgta
     3901 ataattgggc caagttataa tcttttaaat cttgaccata tccacctgta tgaattcccg
     3961 tcaatacaat ttccttatat cctgaattca ctagttgcgt cgcttgttca actacttttt
     4021 ccggatctct tgaacgcatt aagccacgag cccatggaat aatacagaat gtgcagaagt
     4081 tgttacaacc ttcttgaatt tttaatgacg cacgtgttct atctgtaaaa taagggacat
     4141 ctaattcttc atatttacga tttttcatga tatttccaac accattaatt ggttggcgtt
     4201 ctttacggaa ttcgtcaatg taacctaata gtttatgtct atcttgtgta ccaactacta
     4261 catcgacacc aggaatttcc ataatttcag ctgatgaagt ttgcgcataa caacctgtta
     4321 cacagattac agcatcagga ttttgtctta ttgcacgtct aattatttga cgactttttt
     4381 tatcacccgt attcgttact gtacaagtat taataacaaa tacatcagca ttcgcttcaa
     4441 agtcaacgcg ctcatagttt gcttctttaa ataattgcca gattgcttca gtttcataat
     4501 ggtttacttt acaacctaat gtgtgaaacg caactgttga cataaatatt caccccatta
     4561 attctttttc ataacttatt gcacttaacg catacaatgg cgcagtttct gcccgtaaaa
     4621 ttctcggccc aagaccaaca actgtactag tattactaaa taatgaaatt tcattttctg
     4681 acaaaccacc ctcaggacca aaaatcatca acactttatc ctgagcattg aattgttgta
     4741 aagtttgctt gaaattgctt aactcaccat cttttgcttc ctcttcatat gcaataagaa
     4801 tatagtcata attatcaata gtatcacaaa ttaattttaa attcgactcg aattgaatag
     4861 atggaatcac taaacgatag ctttgttcag cagcttcttt aattattttt tgccaacgct
     4921 ctatcttttt ggcaactttt gcctcgttta atttaacaat tgaacgttcc atgctcacag
     4981 ctataaatga tgaagcaccc aattcagtag ctttttgtag caaccactca tatttgtcag
     5041 ctttgattag tccactgcaa atcgtaacat caactggcaa ttctgtatta atattttgtt
     5101 tttcttttaa atcaacttca attttatcac ttgttatgtc agcaatttca cataaataaa
     5161 ctgtttgatc attaaaagtt aaaataattt tactaccaac atcatatctc attacatttg
     5221 ttatatgatg aatatcttct ttttttgtaa taaaaaaacg ctgacttaca tcagcgtttt
     5281 ggtctatgaa ataacgttgc acattattca ctcactttct ggccaacaag acaaacccaa
     5341 ccgttgtcat gttgttctga aataatttta aaacctacac gctccatatg tgactgtata
     5401 ccttcatact tctcttttat aataccagaa gtaataaaat aaccgccttc atttagagta
     5461 ttataagcat cttcaatcat ttcatcaata atatgcgcta aaatatttgc tattacaata
     5521 tcaaattttt ctgtttcgtc tttcaataag ttacctggaa cagcttcaat taacgtttca
     5581 caatgatttc ttctgaagtt ttctttagct acactcactg ccatttcatc aatatccaac
     5641 gctttaatac gttttacacc gattagatga cttgcaatac ttaatatacc tgagccagta
     5701 ccaacatcaa ttactgaatg ctgtggcaat acatatgttt ctattgcctt caaacacata
     5761 cttgtagtcg gatgatcacc tgttccaaaa gccatacctg ggtcgagctc aatgcaaagc
     5821 tcttcatccg cttctttagc atatgtttcc caactaggaa ctattgtgaa cttcttcgac
     5881 gctcggaatg gatggaaata gtttttccat tcattttccc aatccgtctc tgcaataatt
     5941 tgctcactga attgaacgtt atgttgatca agttcatcta aatttaataa ctcatcttta
     6001 atttgctgtc gcaacttatc atcataagtc atttcattaa aataggcttt caatcttact
     6061 cccttatctg gataatcctc ttttttcaaa gcgtaaattt caccgtattt atcttctggt
     6121 tggttaatta aatcatctga atcttctatc acgacaccat ttgatccatg attttcaagt
     6181 atattggtag ccaattctac tgcttcatga ttaataataa ttgaaagctc tgtccagttc
     6241 atactttatt ctcccttaaa gaatcttttt gctctatctt taaaattcga aggttgttca
     6301 ttaatttctt caccatttaa ttgggcaaat tctttcatta gttctttttg tctatctgtt
     6361 aatttagtag gcgttactac tttaatatca acatataaat ctccgtatcc atagccatga
     6421 acatttttta tacccttttc ttttaagcgg aattgcttac ctgtttgtgt accagcaggg
     6481 attgttaaca taacttcatt atttaatgtt ggtattttta tttcatcgcc taaagctgct
     6541 tgtgggaagc taacatttaa tttgtaataa atatcatcac catcacgttt aaatgtttca
     6601 gatggtttaa ctctaaatac tacgtataaa tcaccagcag gtcctccatt cacgcctgga
     6661 gagccttcac cagctaatct aatttgttgt tcattgtcga caccttcagg tactttcact
     6721 tctaatttaa ctgttttatt ttcagtacct tttccgtgac atgttggaca agcttcttca
     6781 aattcttgac cacttccatt acatttagga caaacttgtt cagtacgaac tctacctaaa
     6841 attgtgtttt gttctacagc tacatgacca gcgccattac agtaactaca agtcttttta
     6901 cttgttccag gctttgcacc atcaccatga catgtttcgc atgttacatc tttacggatt
     6961 gaaatttctt ttgttgtacc aaataccgct tcttcaaatg ttaatgtcat tgtatactga
     7021 agatcatcac ctttttgcgg tgcatttgga tctctttgtc tgccgccacc gaagaaagag
     7081 ctaaagatat cttcaaaacc gccgccaccg aagccactaa aaccgccaaa gtcagagcca
     7141 ttgaatcctt gtccaccaaa accttgtgga ccatcatgtc caaattgatc atagcttgcg
     7201 cgtttattat catcacttaa aacttcatag gcttcagaaa tttctttaaa cttttcatct
     7261 gcaccttctt ctttgttaat atctggatga tattttttcg aaagctttcg atacgctttt
     7321 ttgatttcat cttttgaagc atccttacta atgcctaaaa cttcataata atctcttttg
     7381 gccacagcta tctctccttt tcttaattaa ctcatatagt ttaacgtaat atgtcatact
     7441 atccaaataa aaagccaaag ccaatgttct attgactttg acttttcaga tcatgacaac
     7501 attctaattg tattgtttaa ttattttttg tcgtcgtctt ttacttcttt aaattcagca
     7561 tcttctacag tactatcatt gttttgacca gcattagcac cttgtgcttg ttgttgctgt
     7621 tgagccgctt gctcatatac ttttgctgat aattcttgaa tcactttttc aagttcttct
     7681 tttttagatt taatatcttc tatatcttga ccttctaaag cagttttaag agcgtctttt
     7741 ttctcttcag cagatttttt atcttcttca ccgatatttt cgcctaaatc agttaaagtt
     7801 ttttcaactt ggaatactag actgtcagct tcgtttctta agtctacttc ttcacgacgt
     7861 tttttatctg cttcagcgtt aacttcagca tcttttacca tacggtcgat ttcttcgtct
     7921 gataatgaag aacttgattg aattgtaatt ctttgttctt tatttgtacc taagtctttt
     7981 gcagttacat ttacaatacc gtttttatcg atatcaaacg ttacttcaat ttgaggttta
     8041 ccacgttcag ctggtggaat atcagtcaat tggaatctac caagtgtttt attatccgca
     8101 gccattggac gttcaccttg taatacgtgt acatctactg atggttgatt atctactgct
     8161 gttgaataga tttgagattt agatgtagga atcgtagtgt tacgttcaat taacgtattc
     8221 atacgtccac ctaaaatttc aatacctaaa gatagtggtg ttacgtctaa taatactacg
     8281 tctttaacgt cacctgtgat aacgccacct tggattgcag ctcccattgc cactacttcg
     8341 tccgggttta ctcctttgtt aggctcttta ccgatttctt ttttgacagc ttcttgtact
     8401 gctggaatac gagttgatcc accaactaag ataacttcat cgatatctga gtttgttaag
     8461 ccagcgtctt tcattgcttg gcgtgtaggt tccattgttc ttctaattaa tgaatctgat
     8521 aattcttcaa atttagaacg agttaagttt acttctaagt gtaatggacc gttttcacca
     8581 gctgagataa atggtaatga gatttgagtt tgtgatacac ctgataagtc ttttttagct
     8641 ttttcagcag catctttcaa acgttgtaat gccattttat cttgagataa gtctacgcca
     8701 ttttcttttt tgaattctgc aactaggtag tcaataatta cttggtcaaa atcatcaccg
     8761 ccaagtttgt tgtcaccggc tgttgatagt acttcgaata caccgtcacc taattctagg
     8821 atagatacgt caaatgtacc gccacctaag tcaaaaacaa gaactttttc atctttatca
     8881 gttttgtcta aaccatatgc taatgctgca gctgttggtt cattaatgat acgctcaact
     8941 tctaaaccag caattttacc agcatcttta gttgcttgac gttcagcatc gttaaagtat
     9001 gcaggtactg taattacagc tttgtcaact ttctcaccta aatagctttc agctgtattt
     9061 tttaagtttt gtaaaatcat agctgagatt tcttgtggtg tgtatgattt accttcaata
     9121 tctactttat aatcagtacc catatgacgt ttaatagatt gaacagtgtt tgggtttgta
     9181 atagcttgac gttttgctac ttcaccaact tgagtttctc catttttgaa agctacaaca
     9241 gatggtgttg tacgtgaacc ttcagggttt tgaattactt ttggctcatc gccttctaat
     9301 actgttacac atgaatttgt tgtacctaag tctataccaa taattttact cataataaaa
     9361 ttcctccatt taatcattaa attaatttaa ttttaaacaa tgtcttttcg ccaaatttaa
     9421 gttattggtt tactttgacc attgatggtc ttaatactct atctttaagc ttgtatcctt
     9481 tttgtagttc ttgagtgatt tcgccagatt caaaatcagg gttatcatct tgaactacag
     9541 cttggtgaat atttggatca aatgcttcac cttcagtttt aataacttca agaccattat
     9601 cttttagtgc gttaatcaaa ctttcatgca ccatttgtac acctttttga agagatttaa
     9661 aagtctcatc atcaccttca atttgaagtg cacgttctat attgtctatt gctggtaaaa
     9721 tatctgttaa cacacgttgt gcttgatatg ttttgtttat ttcattttct ttttgaattc
     9781 tacgcttata attttcaaac tcagcgtaga gccttaaata tttctcttcg ttttcatctg
     9841 ctaattgttg aagttcatta attttttgat cttttggatc tatttcttca ataacattct
     9901 cgtcagacgt ttcttctatt gcttcatctt gtaaatgacc tttactttct tcagcttgtt
     9961 caactgaatc atcaatattt tgtttgacgt ttgtttcttc aactgttgat tcagtgtttt
    10021 tttcaactga ttcgtcttta tttgtcattt tctgtcctcc aatactttct aatccatcat
    10081 taccaaattc tatttaataa ttgaatgaca ttttgataat gcatagctgt aggtccaatc
    10141 acagcgattt gaccttttaa cgtttcatca aaatgatatt gacttgttac aattgaaata
    10201 tcacttaagc tgtcatcaat ttcattacca atttttacat taatatttgg tgaagatata
    10261 tcttgtaata attctgcaat tctatttgat tctatatatt gtagaatggg ctgaattgaa
    10321 gatacattac tttcattcaa tgcatcaata agtttaacct ttccacccat ataaatgcta
    10381 ttactttgat tagaaatatg attattcatc gtatttaaca atttattgat aaaaatttct
    10441 tcctgctctg attgaacaaa agagacaata tcatcttgta aattctgatt aaactcagtt
    10501 agtttgtttg taacaaaatt tgatattgta tttagtttgt cattattaaa cggtatgtct
    10561 gaagcaagat gtacatgctc aacatgacct gatgaaaata cgataaccat tataactaaa
    10621 ttaggattag cacgaatcaa gtgtacatta ttgataatat cttgtttatg attaggatga
    10681 acaactaaag ttgtatattg agatatattt gataattcat ctgcaaaata tgtcaatgct
    10741 gatgatacat catattgatt ctcaactaac aattgattta atcgtcttaa tttatttgtt
    10801 ttttgatgag atgtttgttc aagtaaacga ttgacataat acctaaaacc taattgtgat
    10861 ggcgaacgcc ctgaagaact atgtgtcttc tcgatatagt ttaaatcttc aagctgtttc
    10921 atctcatttc taattgtagc aggactaaca ttcaagttat gtcgctcaat tagtgtttta
    10981 gaaccaacgg gttgtccaaa atcaacataa tcctcaacaa ttgcgtttaa tatactcaat
    11041 tgcctatctg taatcatgtt ttcacctcat tagcactcac ttatctcaag tgctaattat
    11101 aatttatcaa attggtcaaa gtaagtcaat gttaaagact cgaaatttca atttttttaa
    11161 tcatttatta ggaaagcttc aaaaacctca ttacctatga ctttccctct atttgtaagt
    11221 gcaatcacat cgttcttttc tacaattaat tccttctctt ttaaattatt tattgtttga
    11281 ccaaagacac tttcaataga ttggtcaaac ttctttttga acctactact actcacacct
    11341 tcatttaaac gcaacccaag aaacatttct tcttccattc tctcagtcaa agaaggttta
    11401 tttgatacta aaattgcttt actttcttta tttatagctt tgatataatg attcactgga
    11461 ttgatattcg tataacgcac accatctaca taaccacttg cacctgctcc aaatccataa
    11521 tattcctcat taaaccagta aaccttatta tgttctgatt catggccatc taatgcaaaa
    11581 ttagatattt cgtattgatg gaaaggagat tgttctatct tagacatcag caactgatac
    11641 atgtcagcac ctaaatcctc attaggaagt ttgagcaagc cttttctata catattataa
    11701 aattgggttt taggttcaag tattaagccg taactcgaaa tatgttgaat atccatatct
    11761 aaagctagat ctaaactttg ttcaaaatct tcaatcgtct gtttcggtaa atgatacatt
    11821 aaatctaaac tgattgattt aatacctgtg tttttagcat ttaacaccga agtgtaaata
    11881 tcttcagtat tgtgcgttct acctaaaaca gacaataact ccggcttgaa tgtttgaacg
    11941 cccattgaaa tcctttttac tccatatttc tctaatagtt ggactttctc tttagttaac
    12001 tcatcaggat ttgcttcaaa tgtatactcg cctgtgattg taaacgtatc acgtattgct
    12061 ttaagtaatc tttccaactg attaatagaa agggccgttg gtgtgccgcc acctacatac
    12121 atggtcttta agatcctata ttttgctgta gacatttctg tgattagtgc atctaagtac
    12181 tcatctacag gttgattctg tataaaatat ttattgaaat cacaatatgt acatattctt
    12241 acacaaaatg gaatatgtat atatgcactt tgtaccgtca ttttaatgcc ccgcttctca
    12301 gactttttat ccaatcggct atatcctgtt ggactatctg caattattct tacttttaac
    12361 tttaacgttt aatcaatgat tgtctaatat atcgattcat tttaatattt attcttgttt
    12421 agagggagtg agatagaaat aataaagaac cactaatgat ttattatgta gtggctctta
    12481 cacattagcc agatgtaata tgtccttaaa aataaaatta catgagtaaa actcatgcat
    12541 aagggagtgg gacagaaatg atattttcgc aaaatttatt tcgtcgtccc accccaactt
    12601 gcattgcctg tagaatttct tttcgaaatt ctctatgttg gggccccacc ccaacttgca
    12661 cattatagta agctgacttt tcgtcagctt ctgtgttggg gccccattgt tataagctca
    12721 ttttaactct gtcgatggct gccaatataa cattgtaaag cccaggacgt taatttatcc
    12781 taggctccct ctttttagtt attaaacttt attcattgta aattgttaat tgatttttta
    12841 aaattattca tcatccattt tcaatacagc caagaaagca tcttgtggaa tttcaacatt
    12901 accaactgct ttcatcttag ctttacctgc tttttgtttt tcaagtaatt tacgtttacg
    12961 gcttatgtca ccgccataac atttagctaa aacgttttta cccattgatt taatatttgt
    13021 acgcgctaca attttttgtc ctattgcagc ctgtacaggt acttcaaatt gctgtcttgg
    13081 aattaacgtt ttaagttttt caactaatgc tttaccacgt tcatatgcaa aatctctatg
    13141 aactatgaag cttagcgcat ccactttatc accatttaat aaaatatcca tcttgactaa
    13201 attactttct ttattttcga tgaattcata atcaaatgat gcatatcctt tagtattaga
    13261 tttaagttga tcgaagaaat caaatacaac ttcagctaaa ggtaattcat aaacaatatt
    13321 tacacgaata tcatctaaat agtccatatt tataaattgt ccacgtttac gttgacataa
    13381 ttccattact gcaccgacat agtcatttgg aaccatcata gttgcacgaa catatggctc
    13441 aaatatttta tcaattttat cacgatctgg catttgtgct gggttatcaa ccgtcacttc
    13501 tgaaccgtcc cttaaaacac attgataaat tacagatggt gcagttgcaa ttaattcaat
    13561 accaaattct ctttcaattc tttcttgaat tatttccatg tgtaacatac ctaagaaacc
    13621 agttctataa ccaaaaccta atgcttgtga cgattcaggc tcaaattcta atgatgcatc
    13681 attcaattgt aatttttcta atgcttctct taaatcatta taatttttgt tatctattgg
    13741 gaacagtccg caatatacca ttggattcat tttcttataa ccttgcaatg gttctgatgc
    13801 aggtctacta gctaatgtga tggtgtcacc aaccctagaa tcatcaacat ttttaatact
    13861 tgcaataata taaccaacat caccaactgt taattcatca actggaagct gcttaggtgt
    13921 attaattcca acttctgtta cttcgaactc tttaccagtg gccatcattc gaattttatc
    13981 tccggcttta acaacaccgt ccacaattct tatcgatgaa attacccctc tatatggatc
    14041 atactcagaa tcaaatatta acgcttttag tggtgcttct gggtcaccat ctggagctgg
    14101 cacaacttca actattttct ctagtatctc ttcaattcca atgttagatt tagcacttgc
    14161 taaaacaaca tcgtcttggt ctaaacctat catatcttca atttcttgtt tcacgcgttc
    14221 aggttctgca gcaggtaaat caattttgtt aataacaggc aataactcta actcattatc
    14281 taatgctaaa taaacatttg ctaatgtttg tgcttcgata ccttgagccg catctactac
    14341 taaaatcgcg ccctcacaag ctgccaaaga acgtgacact tcatatgtaa aatcgacgtg
    14401 tccaggcgta tcgattaaat ggaatgtata agtatttcca tctttagctt cgtactttaa
    14461 acgtactgcg tttaatttga ttgtaatacc acgttctctt tctaaatcca ttgaatctag
    14521 taactgatct tgcatatctc ttgtttcaac tgatttggta ttttctaaaa ttctatcagc
    14581 caatgtagat tttccgtggt caatatgtgc tataatcgag aaattcctta tattctctct
    14641 tctttttaag cgttgctcat tatccatttt atccttctca ctttcataaa ataaacccgt
    14701 attatctttc gcatctttga tataataacg tttttaatag gtaaatgcaa acgccataat
    14761 cttggatgaa ctttgaaatt ataacaatct ttatttaaat acatacttgc gatatgttaa
    14821 tatgttaatt acgtacttat aattataaaa cgtattgatt gtgtttccaa cacaatttat
    14881 taaatctgca aaattttaag ctaaccccat caaataaatg attgcacaac ggttagactt
    14941 ttgttaaaat atttcttgtt gtaatcaaat aaaattttga taagatgaac tcacttttag
    15001 gaggtgacag aaatggcaaa tatcaaatct gcaattaaac gtgtaaaaac aactgaaaaa
    15061 gctgaagcac gcaacatttc acaaaagagt gcaatgcgta cagcagttaa aaacgctaaa
    15121 acagctgttt caaataacgc tgataataaa aatgaattag taagcttagc agttaagtta
    15181 gtagacaaag ctgctcaaag taatttaata cattcaaaca aagctgaccg tattaaatca
    15241 caattaatga ctgcaaataa ataatctttt taaataaaag ttcaagcgca tgctt
//
LOCUS       DALAAR010000060        14580 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000060 DALAAR010000000
VERSION     DALAAR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.062"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            64..573
                     /locus_tag="O2458_002004"
     CDS             64..573
                     /locus_tag="O2458_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCZ3887863.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(686..1876)
                     /locus_tag="O2458_002005"
     CDS             complement(686..1876)
                     /locus_tag="O2458_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887864.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(1854..3029)
                     /locus_tag="O2458_002006"
     CDS             complement(1854..3029)
                     /locus_tag="O2458_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCZ3887865.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3462..4943
                     /locus_tag="O2458_002007"
     CDS             3462..4943
                     /locus_tag="O2458_002007"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCZ3887866.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            4927..5187
                     /locus_tag="O2458_002008"
     CDS             4927..5187
                     /locus_tag="O2458_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCZ3887867.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            5187..6749
                     /locus_tag="O2458_002009"
     CDS             5187..6749
                     /locus_tag="O2458_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCZ3887868.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            7051..7854
                     /locus_tag="O2458_002010"
     CDS             7051..7854
                     /locus_tag="O2458_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCZ3887869.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            8073..10397
                     /locus_tag="O2458_002011"
     CDS             8073..10397
                     /locus_tag="O2458_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ3887870.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            10414..11772
                     /locus_tag="O2458_002012"
     CDS             10414..11772
                     /locus_tag="O2458_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ3887871.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            11911..13422
                     /locus_tag="O2458_002013"
     CDS             11911..13422
                     /locus_tag="O2458_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ3887872.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(13602..13690)
                     /locus_tag="O2458_002014"
     tRNA            complement(13602..13690)
                     /locus_tag="O2458_002014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13653..13655),aa:Ser,seq:gct)
     gene            complement(13693..13767)
                     /locus_tag="O2458_002015"
     tRNA            complement(13693..13767)
                     /locus_tag="O2458_002015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13733..13735),aa:Asn,seq:gtt)
     gene            complement(13909..14478)
                     /locus_tag="O2458_002016"
     CDS             complement(13909..14478)
                     /locus_tag="O2458_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCZ3887873.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         5201 a         2039 c         2671 g         4669 t
ORIGIN      
        1 aatgtaacgg ttttcaaaga aatttgatat aatagcaata ggttaaacaa aggaggaatt
       61 cagatgattt taggattagc attaattcca tcaaagtcat ttcaagaagc ggtggattct
      121 taccgtaaaa gatatgataa acagtattca cgaattaaac cacatgtgac aattaaagcg
      181 ccatttgaaa ttaaagatgg tgatttagat tctgtcattg aacaggttag agctcgtatt
      241 aatggtatac cagcagtaga agttcatgct acaaaagctt ctagcttcaa accaacgaac
      301 aatgtgattt actttaaagt tgcgaagacg gacgacttag aagaattgtt taatcgcttt
      361 aatggagaag atttctatgg agaagctgaa catgtttttg tgccacactt tacaatagca
      421 caaggactat ctagccaaga attcgaagat atttttggtc aagtagcatt agctggggta
      481 gaccataaag aaattatcga tgaattaact ttgttacgtt ttgacgatga cgaagataaa
      541 tggaaagtta ttgaaacgtt taaattagct taagtaacat aatagtattg ttaatcgtag
      601 tatgtttgaa ttaataagaa aatggtcatt tttattgaat gtaataaaaa tgaccatttt
      661 ctttatttta aaatacgttt taaccttact tagctttttc tctatttact ataaagtagc
      721 ttccataaaa tacagctaag actaaaaaga ttaatgccga gaaataaaat gtattgttta
      781 aattgttggt aaattgtgta attaatccgc caaataatgg ccctatcatt gagccgaatc
      841 cttggatact attaaaaaca ccccaagttt cttcttgttc atctgatttg ataaatcgtg
      901 ccataaaggt attccatgct ggtaataaga tgccatacat tagaccgata gctaaagcga
      961 taatccacaa gatgtgaata ttaacaatca tagatagagt aaaaattaat atcatgtata
     1021 aaataaatcc gcttagaata acaccataca taaagtttct gctgcggtta tctattagtt
     1081 tcgataaaaa tagcatcgaa actgcacagc cgataccacc aataatgatt gcaacagtat
     1141 attcaattgt gcttacgtta ataaccttag tagcatatgt tggtaatata ggaactaggg
     1201 cagcaattgc ggcaccttgt aaaagaatac cagggaacaa caataaatgg cgctttgtca
     1261 catcaacaat ttgtctcaat tgagctttaa ctggacgagt attataattt gttaacttta
     1321 catcgacaaa ataatataat atccatgcaa ttaaaacgac taaagacatc atgaaggcaa
     1381 agcgtgttgg gtgcactttg ataagtagat tcataaaaac catacctacc aataggccta
     1441 acaaccatga aaaataaaca tagcccattt gtttgccacg tttatcttct tcaacactgg
     1501 ataacataat gacccaaata ggactaactg caataccgag catcatagca ctaaatatga
     1561 ttacaaaagg tgatgctgga aaccaaataa ctaaaaataa acttgtaaat gctaaaataa
     1621 atccagtcgt taaaacgatt tttgtgccga attttttcag taaaaatcct ataacaaagt
     1681 ttgtagatgc atcagcaata aaatgtattg aaaatgctag agacgttatt gctacagcaa
     1741 tggatgtaac tgttggcaag aaattaatat agcttaggat atacatgcct ctcgcaaatt
     1801 ccattaaaaa taagataata agcattaaaa tgaaattttt atgattagcg taattattta
     1861 acgaagaatc ttgcatataa aggaaccttt ccataaatct cttgtggttg tgatgaatga
     1921 ccgattaaat caagtaagtc tcgacatatt gtctgtgtag catacttaat tttatcttgt
     1981 tccattgtac taatcatgtt agttaattgc tcattaccgt tagttaaact tgctacaatt
     2041 tttattgctt cttctggagt atcagcgatt ttaccaaaac ctttttcttc aaagtaaagg
     2101 gcattttcaa gctcttgacc aggtgcagga tttaggaaaa tcattggaat acaacgggcg
     2161 aaaccttcag ttattgtgat accaccaggt ttcgtaatca taagttgact tgatgccatc
     2221 cattcattca tgtgtttggt ataacctaga atcaatacat tctcgttaga tttaaactta
     2281 gctgttaaag aacgctttag ctctttgctc ttaccacaaa tcataactac ttgtgcattt
     2341 gcacttttcg ctaatatatc agtaatcatc gtgtcaaaac ctttagatac accaaatgca
     2401 ccagctgaca ttaaaatagt ttgcttatct ggatctaagt tgttgtctat taaccactgc
     2461 ttttgattaa taggcgtttc aaatttgtta tcaataggaa tacctgtcac tttaactgtt
     2521 gaaggatcaa tacctacgtc tatgaagtct tgtttcgttt cttttgttgc cacataatat
     2581 cttgttgaat acggcgtaat ccagttttta tgtaagcgat agtctgtcat cactgtagca
     2641 actggaatat taatgttaaa ttgctcagtt agtaccgaca taactggtgt aggaaacgtt
     2701 aataatatta aatctggctt ttcttttatc aataaattaa ttaacttatt aagtccatag
     2761 tatttgtaaa aacatttgtc tagtttatct gggcggctgt aataaaaccc tttgtacata
     2821 tttctaaaat atttaaagct attgatatac cattttttac aaatagaagt caaaattgga
     2881 tgagcttcca taaataaatc gtgctcaatg acgcttaaat ggtctagatt catatcatta
     2941 agttgattaa cgatactctg tgtaacttgc atatgaccgt taccgaatga gccagtaata
     3001 atcaatatct ttttattttg agtaaccatt aatagccacc ctccgttagt ttgaaaattt
     3061 tatttaagtg taacttattt tacggcatta taaaagaaat aaagacgcaa agtcgttaca
     3121 tttatagcaa ttttaatcta tagatgaatt gatacaaaat aaaacgttat tttataaagc
     3181 aatttattgt tctatgtttt atttgtatat ttaaaattat ccagtataca attatagcat
     3241 atttttggaa acaattatga tattatacca tgttacaaga tggttttaat aatttaagat
     3301 gagccataat tgtaaaacta attcataata ccgtatgttt tatttttaat agtagaaatt
     3361 agaaaatgct gattagtagg atataacagt gaaattataa atttattaac atcaacaaaa
     3421 cgtgtataat aaacatattg tagaaaaagg agcggttcag tttggatgca agtacgttgt
     3481 ttaagaaagt aaaagtaaag cgtgtattgg gttctttaga acaacaaata gatgatatca
     3541 ctactgattc acgtacagcg agagaaggta gcatttttgt cgcttcagtt ggatatactg
     3601 tagacagtca taagttctgt caaaatgtag ctgatcaagg gtgtaagttg gtagtggtca
     3661 ataaagaaca atcattacca gctaacgtaa cacaagtggt tgtgccggac acattaagag
     3721 tagctagtat tctagcacac acattatatg attatccgag tcatcagtta gtgacatttg
     3781 gtgtaacggg tacaaatggt aaaacttcta ttgcgacgat gattcattta attcaaagaa
     3841 agttacaaaa aaatagtgca tatttaggaa ctaatggttt ccaaattaat gaaacaaaga
     3901 caaaaggtgc aaatacgaca ccagaaacag tttctttaac taagaaaatt aaagaagcag
     3961 ttgatgcagg cgctgaatct atgacattag aagtatcaag ccatggctta gtattaggac
     4021 gactgcgagg cgttgaattt gacgttgcaa tattttcaaa tttaacacaa gaccatttag
     4081 attttcatgg cacaatggaa gcatacggac acgcgaagtc tttattgttt agtcaattag
     4141 gtgaagattt gtcgaaagaa aagtatgtcg tgttaaacaa tgacgattca ttttctgagt
     4201 atttaagaac agtgacgcct tatgaagtat ttagttatgg aattgatgag gaagcccaat
     4261 ttatggctaa aaatattcaa gaatctttac aaggtgtcag ctttgatttt gtaacgcctt
     4321 ttggaactta cccagtaaaa tcgccttatg ttggtaagtt taatatttct aatattatgg
     4381 cggcaatgat tgcggtgtgg agtaaaggta catctttaga aacgattatt aaagctgttg
     4441 aaaatttaga acctgttgaa gggcgattag aagttttaga tccttcgtta cctattgatt
     4501 taattatcga ttatgcacat acagctgatg gtatgaacaa attaatcgat gcagtacagc
     4561 cttttgtaaa gcaaaagttg atatttttag ttggtatggc aggcgaacgt gatttaacta
     4621 aaacgcctga aatggggcga gttgcctgtc gtgcagatta tgtcattttc acaccggata
     4681 atccggcaaa tgatgacccg aaaatgttaa cggcagaatt agccaaaggt gcaacacatc
     4741 aaaactatat tgaatttgat gatcgtgcag aagggataaa acatgcaatt gacatagctg
     4801 agcctgggga tactgtcgtt ttagcatcaa aaggaagaga accatatcaa atcatgccag
     4861 ggcatattaa ggtgccacat cgagatgatt taattggcct tgaagcagct tacaaaaagt
     4921 tcggtggtgg ccctgttgat taataaaaga tttattgatg aaggtaaaac tattgatgtt
     4981 tatttattcg aagcattaaa taaccagata atcattgcta taccagattg gttttggtca
     5041 tatcagatgg caatgacatt agatgaagaa acttgttttg aagcaatact catgcaattg
     5101 tttgttttta aagaagagga agaggcagaa tcgattgcat cacaactaac agattggata
     5161 gaaacatata aaaaggagaa agactaatga acttaaagca agaagttgag tctagaaaga
     5221 cttttgcgat tatttcacat cccgatgcag ggaaaacaac gttaactgaa aaactattgt
     5281 acttcagtgg tgctattcgt gaagcgggta cagttaaagg gaagaagact ggtaaatttg
     5341 cgacaagtga ctggatgaaa gttgaacaag agcgtggtat ttctgtaact agttcagtaa
     5401 tgcaatttga ttacgatgat tataaaatca atatcttaga tacaccagga catgaagact
     5461 tttcagaaga tacgtataga acattaatgg cagttgacag tgctgtcatg gtcatagact
     5521 gtgcaaaagg tattgaacca caaacattga agttatttaa agtttgtaaa atgcgtggta
     5581 ttccaatctt tacattcatt aataaattag accgagtagg taaagaacca tttgaattat
     5641 tagatgaaat cgaagagaca ttaaatattg aaacataccc tatgaattgg ccaattggta
     5701 tgggacaaag tttctttggc atcattgata gaaagtctaa aacaattgaa ccatttagag
     5761 atgaagaaaa tatattacat ttgaatgatg attttgagtt ggaagaagat catgcaatta
     5821 caaatgatag tgattttgaa caagcgattg aagaattaat gttggttgaa gaagcgggtg
     5881 aagcctttga taatgacgcg ctgttgagtg gagacttaac acctgtattt ttcggttcag
     5941 ctttagctaa ctttggtgta caaaatttct taaatgcata tgttgatttt gcgccaatgc
     6001 caaatgcgag acaaacaaaa gaagacgttg aagtaagccc gtttgatgat tcattttcag
     6061 gatttatctt taaaattcaa gccaacatgg accctaaaca ccgtgataga attgccttta
     6121 tgcgtgtcgt tagtggtgca tttgaacgtg gtatggatgt tactttgcaa cgtactaata
     6181 aaaagcaaaa gatcacacgt tcaacgtcat ttatggcaga cgataaagaa actgtgaatc
     6241 atgctgtagc aggcgatatc attggactat atgatactgg taattatcaa attggagata
     6301 ctttagttgg tggaaaacaa acctacagtt tccaagattt accacaattt acgccagaaa
     6361 tttttatgaa agtttctgct aaaaacgtca tgaaacagaa gcatttccat aaaggtattg
     6421 aacaattagt acaagaaggt gcgattcaat actataaaac attacacaca aaccaaatta
     6481 ttttaggtgc tgttggtcag ttacaatttg aagttttcga acatagaatg aaaaacgaat
     6541 ataatgttga tgttgttatg gagccagtag gccgtaaaat tgcacgttgg attgaaaatg
     6601 aagaccaaat tacagataag atgaacacat caagatcgat tttagtgaaa gatagatatg
     6661 acgatttagt attcttattt gaaaatgaat ttgcaacaag atggtttgaa gagaaattcc
     6721 ctgaaattaa attgtatagt ttactttaac agctcaattg tataatcgaa tttgttacat
     6781 taaaaataat tgtttcgttg aagaaaaata aattgtatat tttaaaagaa aaaggtatac
     6841 tatgatgtat caaatgaata acctatggca ttttgtcaga ggggagtaac ttaagaatca
     6901 tgaccgtata aatgattcga cactttatcg tcattacgaa gatatcttcc ggtaaagtgg
     6961 gcaatttaaa ttgcttagtg agacctttgc tatttattta gcataggtct ttttgtttgt
     7021 acttaactta tttatttaaa ggagttgtac atgttaatgg atccaagttt gatcttacct
     7081 tatttatggg tacttgtcgt tttagtattt ttagaaggct tattagcagc agataacgcg
     7141 attgttatgg ctgtaatggt taagcactta ccacccgaac aacgtaaaaa agctttgttt
     7201 tacggtttgt taggtgcatt tgtatttaga tttttagcat tattcttaat tagtattatc
     7261 gcgaactttt ggtttattca agctgcagga gcggtttact taatttatat gtcaatcaaa
     7321 aatctgtggc agttctttaa acacccagaa attgaaagtc ctgaagctgg agatgatcat
     7381 cattatgatg aatctggtga agagattaaa gcaagtaaca aatcattctg gggaactgtg
     7441 ttgaaaatag aatttgcaga tatcgcattt gccattgatt ctatgcttgc tgctttagct
     7501 attgctgtaa cacttcctaa agttggtatt cactttggtg gtatggactt aggtcagttc
     7561 gtagtcatgt tcctaggtgg aatgattggt gttattctaa tgcgttatgc agcaacatgg
     7621 tttgtagagc tattaaacaa atatccagga cttgaaggtg cagccttcgc gatcgttggt
     7681 tgggtaggtg ttaaattagt tgtcatggta ttagcgcacc cagacatcgc tgtattgcct
     7741 gagcacttcc cacatggcgt attatggcaa tctattttct ggacagtact aattggatta
     7801 gtaattatcg gttggttagg ttcagttgtt aaaaataaaa aatcgcataa ataattgatg
     7861 tgaagcggac aatcttaatt tagtttaagg ttgtcctttt tcatttaatt gagtgattta
     7921 tgaaaaatgg attttgaaga atgtgaatca aaagatgcga tatagtatta agaaaatgtg
     7981 ccttttatat ttagcatttt ttcaatagaa attatataga ttttaaagca aattaggtgt
     8041 taatgtgtca taatgataag tgattttatt gaatggagtg gacattagtg gatattggta
     8101 aaaaacatgt aattcctaaa agtcagtacc gacgtaagcg tcgtgaattc ttccacaacg
     8161 aagacagaga agaaaattta aatcaacatc aagataaaca aaatatagat aatacaacat
     8221 caaaaaaagc agataagcaa atacataaag attcaattga taagcacgaa cgttttaaaa
     8281 atagtttatc atcgcattta gaacagagaa accgtgatgt taatgagaat aaagctgaag
     8341 aaagtaaaag taatcaggat agtaagtcag catataacag agatcattat ttaacagacg
     8401 atgtatctaa aaaacaaaat tcattagatt cagtggacca agatacagtg aaatcaaaat
     8461 attatgagca aaattctgaa gcgactttat caactaaatc aaccgataaa gtagaatcaa
     8521 ctgaaatgag aaagctaagt tcagataaaa acaaagttgg tcatgaagag caacatgtac
     8581 tttctaaacc ttcagaacat gataaagaga ctagaattga ttctgagtct tcaagaactg
     8641 attcagacag ctcgatgcag acagagaaaa taaaaaaaga cagttcagat ggaaataaaa
     8701 gtagtaatct gaaatctgaa gtaatatcag acaaatcaaa tacagtacca aaattgtcgg
     8761 aatctgatga tgaagtaaat aatcagaagc cattaacttt accggaagaa cagaaattga
     8821 aaagacagca aagtcaaaat gagcaaacaa aaacctatac atatggtgat agcgaacaaa
     8881 atgacaagtc taatcatgaa aatgatttaa gtcatcatat accatcgata agtgatgata
     8941 aagataacgt catgagagaa aatcatattg ttgacgataa tcctgataat gatatcaata
     9001 caccatcatt atcaaaaaca gatgacgatc gaaaacttga tgaaaaaatt catgttgaag
     9061 ataaacataa acaaaatgca gactcgtctg aaacggtggg atatcaaagt cagtcaactg
     9121 catctcatcg tagcactgaa aaaagaaata tttctattaa tgaccatgat aaattaaacg
     9181 gtcaaaaaac aaatacaaag acatcggcaa ataataatca aaaaaaggct acatcaaaat
     9241 tgaacaaagg gcgcgctacg aataataatt atagtgacat tttgaaaaag ttttggatga
     9301 tgtattggcc taaattagtt attctaatgg gtattattat tctaattgtt attttgaatg
     9361 ccatttttaa taatgtgaac aaaaatgatc gcatgaatga taataatgat gcagatgctc
     9421 aaaaatatac gacaacgatg aaaaatgcca ataacacagt taaatcggtc gttacagttg
     9481 aaaatgaaac atcaaaagat tcatcattac ctaaagataa agcatctcaa gacgaagtgg
     9541 gatcaggtgt tgtatataaa aaatctggag atacgttata tattgttacg aatgcacacg
     9601 ttgtcggtga taaagaaaat caaaaaataa ctttctcgaa taataaaagt gttgttggga
     9661 aagtgcttgg taaagataaa tggtcagatt tagctgttgt taaagcaact tcttcagaca
     9721 gttcagtgaa agagatagct attggagatt caaataattt agtgttagga gagccaatat
     9781 tagtcgtagg taatccactt ggtgtagact ttaaaggcac tgtgacagaa ggtattattt
     9841 caggtctgaa cagaaatgtt cctattgatt tcgataaaga taataaatat gatatgttga
     9901 tgaaagcttt ccaaattgat gcatcagtaa atccaggtaa ctcgggtggt gctgtcgtca
     9961 atagagaagg aaaattaata ggtgtagttg cagctaaaat tagtatgcca aacgttgaaa
    10021 atatgtcatt tgcaatacct gttaatgaag tacaaaagat tgtaaaagat ttagaaacaa
    10081 aaggtaaaat tgactatccc gatgtaggtg ttaaaatgaa gaatattgcc agtctaaata
    10141 gttttgaaag acaagcagtt aaattgccag gaaaagttaa gaacggtgtt gttgtagatc
    10201 aagttgacaa caatggttta gcagatcaat ctggtctgaa aaaaggtgat gtaattactg
    10261 aattagatgg caaactttta gaagatgatt tacgctttag gcagattata tttagtcata
    10321 aagatgactt gaaatcaatt acagcgaaga tttatagaga tggtaaagag aaagaaatta
    10381 atattaaact aaaataatgt tgaggtgaac aacgtgtcaa tttttagcca gtttttaaaa
    10441 agatcaagcc ctcaacaagg tattgtattg tactatatcg tcgcaattgt cattgcattt
    10501 ttattattaa acttaccgta tgttcataaa ccaggtgtag aagtaaatcc aattgacaca
    10561 ttatttgttg ccgtatccgg aattagtgtt actggattgt ctccgataag tattgtcgat
    10621 acctattcta catttggaca attaattatc ctcgtgatat taaatattgg tggaattggc
    10681 gtcatggcaa ttggtacgat gttatgggtg gtactaggta aacatattgg aattagagaa
    10741 cgtcagttaa ttatgttaga taataacaaa aacacaatga gtggtaccgt caaattgatt
    10801 attgatattg taaaatcaat atttgtaatc gaactcgtag gagccatgtt attagcattt
    10861 tacttttatc gagataatcc agatttaaaa tatgcaatca tgcaaggtgt ttttgtttct
    10921 atttctgcca ctaccaatgg tggattagat attacaggta agtcattaat tccttatgca
    10981 catgattatt ttgtacaagc gatagttata tttttaataa ttttaggatc aatcggcttc
    11041 ccagtattat tagaagttaa agcttatatt caaaataggg ttactaattt tagattttca
    11101 ttatttacta aaattacgac atcaacatat ttattcctat ttattgttgg ggtattagcc
    11161 attctattat ttgaacataa ccatgcgttc aaaggtttaa gttggcatca atcgttattc
    11221 tattcgctgt ttcaatcagc gactacaaga agtgcgggtc ttcaaacaat tgatgtgaca
    11281 acactaagtg accccactaa tattatcatg ggtattttaa tgtttatagg atcttcgcca
    11341 agttcggttg gtggcggtat tcgtacaaca actttcgcta ttttaatttt gtttttaatt
    11401 aactttagta ataatgccga taaaacatcc attaaagttt acaatagaga agtacacatt
    11461 atggatattc aacgttcatt tgcagtattt acaatggcga caattttaac atttttagga
    11521 atgctaatta tatcagctac tgaaaatggt aagcttacat ttttacaagt attttttgaa
    11581 gtcatgtctg catttggaac ttgtggacta tcgcttggtg tcacaagtga tattagtgat
    11641 atttctaagg tcgtactaat gatactcatg tttataggac gtgttggctt aatatcattt
    11701 atcattatga tagcaggacg tcgagaacca gataaattcc attatccaaa agaacgtatt
    11761 caaataggat aatataatag caatctaagt ttagttaatg tagattttaa ctggaactta
    11821 gattgctttt ttagtttgta tttttaactt attttataag acgattggtt tcgaaaatgg
    11881 taaactagta acaatgagag gtgtaacatg atggaaaaaa atgaaaacat taatgtagag
    11941 attttaacta cgtcagatat gcatagtcat ttcttaaatg gtgattatgg ttcaaatatt
    12001 tatagagctg gtacttatgt taaccaagta agagcacaaa atcatcgcgt cattttatta
    12061 gatagtggcg gaagtttagc tggctcgtta gcggcctatt attatgctat tgttgcacct
    12121 tataaacgac atccaatgat aaagttaatg aacagaatgc attatgatgc tagcggtgtg
    12181 agtccaagtg aattcaagtt tggtttatca tttttaactc gttcaattgc tttggcacgt
    12241 tttccatggt tatcagcaaa tattgaatac aatgttacta aggagcctta tttttcaact
    12301 ccatattgta ttaaacattt tggtgactta aaaattgcta tcgtaggcgt cacagcagat
    12361 ggtttaatgg aaaatgagta ttctgaaatg gagcaagatg tatctattga aaagacatta
    12421 gtggcatcaa aacgttggat tagatatatc catgaagttg aagagccaga ttttttgatt
    12481 gtaatttatc atggtggatt gaataaaatt agtaatagta cgaaaaataa aaaggcaagt
    12541 tcgaatgaag ctgaaaaatt aatggaagaa ctcggtgtta tagatttaat gattacagct
    12601 catcagcatc aaacaatagt aggtcaagat catgaaacgt attatgttca ggctggtcaa
    12661 gatgccaaag agcttgtaca tctttcgatt aattttaaaa agagaacaac aacttatgat
    12721 gttgaaagca ttgattctaa agtgattgac ttaaatgagt atgaagagga tcaagaatta
    12781 ttagatttaa cattctatga tagaaaagca gtggcttatt ggtcacagga aatcataagt
    12841 gataaaggtt tgatgttatc agtaaatggg ttacaagatt tagtctgtca aacacatcca
    12901 ttttcgcaat tattacatga tgcaattcac cttgcatttg ataatgatat aacatgtgtc
    12961 cacgtgccta tgaacggaga gaaggggttg agtggacaga ttcgaaatga agatttgtat
    13021 catgcatacc catatccaga taagccaatg gatatgacaa ttagtggtca aaatatcaaa
    13081 gatatattgg agtatagtta ttcacattta gattttgtta acgagcaatt aagcttaaca
    13141 attattgatg aaacgttatg tacaatgtgg caaggattca attatgagat tgatatgaat
    13201 caagaacctg ggcaacgagt aatgttagat caaattgatt tgactaagag ttatagagtt
    13261 acaatgactg actattgtta tcgtaactac aagaattatt taaaaaatgc tattatacat
    13321 gaatcatacg atgaaacaat gagtacatta attgcagaga agttaagaga tccgaattat
    13381 cgtatttcat gtagtgataa ttttatagtt aaaaacaggt aaatattgct atgtaataaa
    13441 ttaattataa gttcaacaag tataaaaacc tttcttagag aagattatct aggaaaggtt
    13501 tttagaattt taaaattgat ataaaagaac agtgaaattc tcattcatta tatataagta
    13561 aacaaaaaag ctaaaatggt atataccatt ttagcaagta acggaaacgg agggattcga
    13621 accctcgcgc cgctttcgcg acctacaccc ttagcagggg cgcctcttca gccaacttga
    13681 gtacgtttcc aatggctcca caggtaggac tcgaacctac gaccgatcgg ttaacagccg
    13741 atagctctac cactgagcta ctgtggatta atttgtatgt gtttgaatat caagcacaaa
    13801 tattattata acaagattaa gttaatttgc aatagatacc ttaaaaaaat atgcagaaca
    13861 tttacactat atttataaaa cattccgcat attttaaaaa taaacaattt atttatcttc
    13921 aaataaagaa tagcgtgtca atgcttcaaa cacattcaat gtggctttat tataatctat
    13981 tggttggtcg ggattttttg atatcatgcg agattgttta tctaccatgt aatagtaaaa
    14041 aatggaattg ttatattgat gccataaact gtggtatgaa acatgaagaa tgattgattc
    14101 attattaata aatgtcactt tacatttacg attttttaat tctttaatac tttcgatata
    14161 atgcatattt aaccaaatat tttcattttg tctgtcagaa tgtgttggga aaaaataagt
    14221 tggaaataat ggtgttagta aaataggtgg tttactagaa atgccagtaa tgcggtttgt
    14281 ctctgctttc ttgccaagat aagtattacc ataaaatttg caagagcgtt caatgatttt
    14341 ctggacttta aaaggatttt gaatacgcgt tttgtcaaac cgaattattt cactaccgtt
    14401 tctttgatcg tcatcatcaa atgctggtcg aataaccatg tctcctttgc gtatcacata
    14461 aatattttga gaatacataa gcaaaacctc gctttattaa gttttaaacc attaatataa
    14521 aaatacaatt aatgatataa agatatttta taataaaatt ctgagttgtc aagttattat
//
LOCUS       DALAAR010000061        14123 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000061 DALAAR010000000
VERSION     DALAAR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.063"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(168..860)
                     /locus_tag="O2458_002017"
     CDS             complement(168..860)
                     /locus_tag="O2458_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ3887874.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1096..1554
                     /locus_tag="O2458_002018"
                     /pseudo
     CDS             1096..1554
                     /locus_tag="O2458_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1541..1843
                     /locus_tag="O2458_002019"
     CDS             1541..1843
                     /locus_tag="O2458_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887875.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            1997..2356
                     /locus_tag="O2458_002020"
     CDS             1997..2356
                     /locus_tag="O2458_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887876.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2441..3052)
                     /locus_tag="O2458_002021"
     CDS             complement(2441..3052)
                     /locus_tag="O2458_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ3887877.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3131..3607)
                     /locus_tag="O2458_002022"
     CDS             complement(3131..3607)
                     /locus_tag="O2458_002022"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCZ3887878.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3610..4110)
                     /locus_tag="O2458_002023"
     CDS             complement(3610..4110)
                     /locus_tag="O2458_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887879.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4345..5112)
                     /locus_tag="O2458_002024"
     CDS             complement(4345..5112)
                     /locus_tag="O2458_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCZ3887880.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5109..5801)
                     /locus_tag="O2458_002025"
     CDS             complement(5109..5801)
                     /locus_tag="O2458_002025"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCZ3887881.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5818..6480)
                     /locus_tag="O2458_002026"
     CDS             complement(5818..6480)
                     /locus_tag="O2458_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCZ3887882.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(7328..7888)
                     /gene="icaR"
                     /locus_tag="O2458_002027"
     CDS             complement(7328..7888)
                     /gene="icaR"
                     /locus_tag="O2458_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCZ3887883.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            8052..9290
                     /gene="pgaC"
                     /locus_tag="O2458_002028"
     CDS             8052..9290
                     /gene="pgaC"
                     /locus_tag="O2458_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCZ3887884.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            9254..9559
                     /gene="icaD"
                     /locus_tag="O2458_002029"
     CDS             9254..9559
                     /gene="icaD"
                     /locus_tag="O2458_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCZ3887885.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            9556..10428
                     /gene="icaB"
                     /locus_tag="O2458_002030"
     CDS             9556..10428
                     /gene="icaB"
                     /locus_tag="O2458_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCZ3887886.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            10415..11467
                     /gene="icaC"
                     /locus_tag="O2458_002031"
     CDS             10415..11467
                     /gene="icaC"
                     /locus_tag="O2458_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCZ3887887.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(11914..13956)
                     /gene="lip1"
                     /locus_tag="O2458_002032"
     CDS             complement(11914..13956)
                     /gene="lip1"
                     /locus_tag="O2458_002032"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCZ3887888.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         4552 a         2422 c         2039 g         5110 t
ORIGIN      
        1 aataaaccta gtgtaatata tgtgtaattc taaaagattc ttctttaaaa atataaatac
       61 cacgacatat tgctttaaca ttttcattta taaagcgaaa aaatgcatcg ctactaagtt
      121 gaatgtttag taaggatgca tttaattcac taaaatgatt aaattactta tatcttttca
      181 tctgattgat tatcgaaatt tcttccttct aaacctgcta actcttcttt agaagctgca
      241 ggtgctttca tttcaaatat ctcattcact actgtgtaat cgtaatatcc taatctggca
      301 ataggtttaa tcgacttaat gtccaattta ccattatcaa gaataacctt atcgtcaata
      361 tgaacttggg caactcttcc tataacaata tctacggtag atactggatc tccagttgga
      421 atacgaatcg tttgaacgta ctcacattca aaatgaactg gcgattcttt tacacgatat
      481 cctggagctt ctatacattt ttcctttgtt acatctgcaa aattaaattc atcctcttct
      541 ggtggcaatg ctttcgatga taaattaact gcttctctta aatcatacgt tgccatattc
      601 cacacaaacc aacctgtctc ttcagcattt ttcactgtat ctttacgttc gtgatcacca
      661 agaacggatt gatttgctgc gaacataacc ataggcggat cccaagttaa gttttgatac
      721 tgactataag gcgctaaatt atctttccca tctttcgata cagtagagat ccaccctatt
      781 ggacgtggta ctgtactact tttaaatggg tcgtgcggta aaccatgact tcttacacct
      841 tgttttggcg aataattcat actatcttca ccccttataa gtaattacat ttaaggttac
      901 gccctcttta cataagcgtc taatataaat aaacaattta tttataagta gaaactatat
      961 atgacgtggt tgcttataat ttgcgttctt gattcgaaaa attcagataa ggatttatac
     1021 aattaatatt tatgatatct tttgtaaatt taattaatta tagttacttc aatcatgatt
     1081 agtttataat aataaagtga aattgaaaaa gacagctatt atgcgatgag cgaaaaactt
     1141 caagtaaaac aagaatttaa cggcgttaca aggcaatact tgaatttcag aacagatagt
     1201 atcgatgatc tggataagtt caaaaaagaa ttcaaacctg ttatgcactt aaaaggtgat
     1261 gcattcaatc aacagttaca atctttgatt aacaaatatc cacaaataca aaaaaatatg
     1321 aaatcagagt tcattgctta ttatgataaa gaaaaaaata gagaaacagt aaaaaactat
     1381 gcttggaacc ttcaaaaatc tataaatgac attatgcaat catatcctag cacaaaattt
     1441 gtacagtttt ataaagatga tgtttccccg tcaatggtag atggaaatgg ccgtttaaaa
     1501 tcggatacta atgtaatttc catcgaggga ggaaagtatg atgagaacaa atgatcatat
     1561 aaccgattta gatgctattg agttaagtag cggttggaat tacaattcga aattggagac
     1621 tggcaaaaat gcactactat cagatagcct tttaagagat attaaagtat taagttctaa
     1681 tgctaaaggc gacgctgaca tactagaaac agaacttcgt gctgtaataa actcaatgtc
     1741 tcaaaacgaa ggtaaatctg ttgatgcact taaaaatagt accgcttctc taaaaaacaa
     1801 tctaaccaaa ttaaaagagc aactcgacaa aactacacat taatttcata cattttgagt
     1861 gaacagaatt atatttccat agcaaacatt cctaaactac acatacgtta cataattgat
     1921 tcatttttat agaaacgggt aaaaatgata aagtaatcat ctttcacaaa aaatcaaata
     1981 ttgggaggaa ttaaatatga ttttgcgtta cgcaacgaat ttaaaacttg cgaaagagct
     2041 ataccaagcc agtaaaccta aattgcaaga agataagggt atgattgaac tgtttgaaca
     2101 tacttttggc ttgcaaaaag aacttgtaaa atacgtcggc attgcagaag caactactgc
     2161 tgctttatac tcagctagct ttatcaataa aaacatttct cgccttgcat cactatcaac
     2221 gataggcatt ttaagtgtag ctgcttacaa acatttcgaa gctggccaag gtaaaaaagg
     2281 tgcacaacat gcattaaatt tattaggtct tgctacttta agtttactag atacattttc
     2341 ttgtaataaa aaatagtctt cattttagaa gcacatactt tagcacctgt gtacttttga
     2401 ttcaattcat aaacggtata ccgctatagc atgtgtttta ttatttttct ggtctcttta
     2461 tcgtaaatat tccccctaat ttagcataat catttccggc taaacgagaa acagcctgta
     2521 atgcgtgtgg attgatttta aaatcaccct cataaatact gtcatcaata tgataagtta
     2581 caatttcacc tattattaaa tcagccccat ctaatacatc tccaagcaat atcatttgcg
     2641 atagtttaca ttcgaatctc attttcgcat ctttaattcc tggcgtctta atcgttgtag
     2701 atgttaaaag tgataattct gtacgactca actcactgtc accatatgct aacggcgctg
     2761 cagtctcatt aatatcttga acattatctt cgtctgtaat atgcacaaca aagtctccag
     2821 tccgttctat atttaatgca gtatcttttc tcttacctcc tgcacgttga actgcaatag
     2881 caatcattgg cggatgatta ttaacaatat taaaaaagct aaatggtgct gcatttactg
     2941 atgcatcttg atttaatgtt gtaacaaaag ctataggtcg tggaataatt gaaccaatta
     3001 ataatttata gttttctcta gcagttaatg attgtgcatc aaacgtatac ataataccta
     3061 cctcttttct aagtatatct aggtatttct ccgattttgg ttaatttaaa catctattct
     3121 cctctgaaaa tcacttgtat ttatttagca aatcttttga aatatgacac atatgcatat
     3181 cttctggata tttttctaaa tgttgctgat gttcttcagc acttttaatg tagttagaca
     3241 gcggtaagac ttccactgca atttgatctc tgtctttacg tcgttcaatg aactgacgcg
     3301 cttcaattaa gtggtcatct acacaactat ataaacccgt tcgatacttt tgtccaatat
     3361 catttccttg ttgattcaca ctgtaaggat caatgatttc aaataaataa ttcataatgt
     3421 ctgtaattgt taacatacga tcatcgaaat gaagtttgac acattcagca taaccatcat
     3481 acggaccgtc taatttagag cttcttccat ttgctcttcc tgcttctgta tgtataattc
     3541 caggtattgt tgcaaaaaat gcttcaacac cccataaaca tcctcctgct acataaacaa
     3601 ctgccatatt tacacctcat catccttttt tatattttta acaaggttat accatttaat
     3661 accgccatga catgattctg atacaccttc attacgatac ccatattttt cataaaatga
     3721 aattaatgat tctcgacatg ttaacgttac accatgtcga tgatgattct tagcaagagt
     3781 ttcaaaatag tttagtaagc gacctgcaat accctgacct tgataatttg gtgctacaac
     3841 aagacctaac acactaatat agccaccttc actattattt gtggagacat ttttaaataa
     3901 atcatcgcta atgtaacgct cttttatgac tggaccgttg atgtatccaa ttacttcatc
     3961 attcttttca gctacaataa aagtttcttg aatttgttca attctcaatt tcaatgcctc
     4021 tcgtgttgca gcttcttccg gtgaaaaacc aatattttca agtgcaataa tttgatctaa
     4081 gtcctgcatg attacttttc ttaataccat aaaaacactt ccttagccct atatatcaaa
     4141 agtaatacct tcatctttat attcattatt ttcaaactac cttcactata aaaaacgagt
     4201 ctttttattg aaagccattc gccttaatca attagacaag ttgtattata cgaattagta
     4261 aataatcatg attataattc attttaagca tactcaaaaa gactggtaca tgtataccag
     4321 tctaattcga aaaaatatat ttagttaaaa ccatttcaaa aacgacttca cttttcctgg
     4381 cttagtcggt ctgtctaaat aaacaaaatt atcattgatt actgaactgg catttcgaag
     4441 taatgcttca atatcattcg aatatttctt caatttatga ttgtgaaata attcttgcat
     4501 caaaaatggt ctttggtcac atgaatgtgc atctgaagct acaaaatgag ccaaattaca
     4561 ttctataaat tgtaatgata acttttgaat gtttttacca aatccaccaa ctaaagaact
     4621 cgatgttaat tgactcagtg ccccatttgc aaccaattca tataatattt ccggattttt
     4681 ggcgatactt ctatttcttt caggatgtgc aatgattggt atgtaacctc tcgattgtat
     4741 ttcaaaaaac aattgttttg tataatgtgg tacttcgccc gttggaaatt caattaataa
     4801 atatttcgaa cgattaatac cttgaatact accattatct aagcctttca gaatcgaatc
     4861 tgtaattcta atttcttgcc cgggaaataa tttaatatcc aatgcttgaa cttctggatg
     4921 cgttcttaac tccgccaatt tcacaagcac ttgttgaaat gtattatcat atctcggatg
     4981 caaatgatga ggtgtcgcta caatacttgt tacaccttca tccttagctt gctttaatag
     5041 tgcaatactc ttttcaattg ttttaggacc atcatctata tcaactaata tatggttatg
     5101 aatatcaatc atgattcatc agtcccataa tatgcatagt aactagcact tttatcttta
     5161 ggcattctat ttaagactac acctaataat ttagcacctg ttgcttcaat aagttctttt
     5221 ccttttttaa cttcatctct attattattt tccgaattaa ctacgtagac aacattgccg
     5281 gtaaactttg aaaataattg cgcatctgta actgtgttca ctggtggcgt atcgataatt
     5341 acaaagttat aattcatcaa taatgtgtca tacaaatttg caaatgccct tgatgtaatt
     5401 aactctgacg gattcggtgg gattggccca gacgtcaaga cgtctaaatc ttgaatttca
     5461 gttgagataa tactgtcttg ataagttgac caatttagca ataaacttga taggccttca
     5521 ttgtttggca aattaaaaat ataatgctgc gtaggtttac gcatatcccc gtctacgatt
     5581 agtgttttat aacctgcttg cgcatatgca actgctaaat ttgctgcaat tgtagactta
     5641 cctgcgcctg gtgcctctga agtgattaca atgctttgaa ctgcactgtc aggatttgca
     5701 aacataatat ttgatcttat gcctcgaaat ttctcgctaa taggtgactt tggttgttca
     5761 tggacaatta aacttgatgt acttcttcgt gtattcgtca tggtaattcc tcgtaaatta
     5821 aaatttttgt attgaaccta aaataggtaa tcctagttgc gattcaacat cttcttctgt
     5881 cttaatacgc ttatctaata attcttttaa gaaaataatc aatattgcta aaacaatacc
     5941 aacaataatg ctgataacta agttgacaga tactattgga gatactttta cagcattatc
     6001 atgtgctgag gaaagtatcg taacattatc aacactcata attttaggca tgtcatgagc
     6061 aaaaacttta gatattttat taacaatttt gtcagattca gatttattcc cagtggtaac
     6121 tgatacagta ataatttgag agtttgtttg attggttact tttaaaaatg aattcaactc
     6181 agctgttgaa tactgaccat caaattctct agatacttta tctagaattc taggactttt
     6241 gataatttcc gtatatgtat taacagactg caaactactt tgaacatttt ggaaagctaa
     6301 atcacttgag gactttttca tgttcactaa tatttgagta gaagcagtat atttgtcagg
     6361 cataacaaaa aaggttaatg ccgcacttac tacaagacat attgccggta aaataagcaa
     6421 taatttaata ttcttcttta gaatatttaa tagttttact aaatcaaact tttctttcat
     6481 ggtttcctcc acataatcaa tcattgtatt cattatgtat gttttataaa tcggacaatt
     6541 atatctagtt taacgaccac aaaacataca caactacatt ttctctaatt atttatataa
     6601 atattttatc gtttaaaatt atatcatgat tctctaccat tatgtataac ttatttatat
     6661 ttttgcacaa gatataatat tgtccaactt taaatatcca aacctattaa taataaaact
     6721 agataccatc gtactctatc atggctttct tataatcgag tagaagcatc atcattactt
     6781 gattatttgc tctttacaac accgagcgtg cccgtactcg gtaattcaat accttgcgta
     6841 acccgtcact gtgagttggg ttaatgataa taaagcccac accttttaaa aagatgtggg
     6901 taatttatat aatttttatt tacattttta acttataaaa aaagcgccta tgtcatgatt
     6961 taccatcaca taggcgctta tcaataaatt attacttatt actttccatt tcatctaatt
     7021 tatgcggatt tcctgtaatt agatgacaac ttattctttt caggggaaca ttacactttt
     7081 ataatatgtt caaagacaaa cttaaccatt cacaaatata aagaataata ttatcaaatc
     7141 attgaacaaa tcgtattttg caacaattga tatttatatt aatgtattgc atttaattta
     7201 taaaattcat atacatctta atattctcaa tatcgatttg tattgtcaac tttatataga
     7261 tttaaaaaaa taatctcatg tcttttttta caaaagtaag ttaattatta caaactagta
     7321 acaaaaatta tttcttcaaa aatatattta gtagcgaata cacttcatct ttgaattgac
     7381 ttttactttc ttccactgct ccaaattttt gcgaaaagga tgctttcaaa taccaacttt
     7441 caagaaacag caatattaaa ttctgaaagt cttcttttgt catctttatc tttgattcat
     7501 catagaattt tgctatctct ttacttaatg attgatttaa atcttgtatt tgtccgtaaa
     7561 tatttccaga aaattcctca ggcgtattag ataattgaac gtacattcta atataccttt
     7621 cttcgatgtc gaaaataaac tcaaataaga attgatataa agcatcaatt gaatagttcg
     7681 atttattttg attcatcata ataatattat taaggtaatc aaaacaacat ttaacacttt
     7741 gttcgtaaat actttttttc gagtcaaaat ggtaatataa actcgctttc tttatattta
     7801 cacttttagc tatatcatca agtgttgtac cgtcataccc cttctctgaa aataaggtta
     7861 ttgcgttatc aataatctta tccttcaatt tttataaccc cctactgaaa attaatcaca
     7921 ctatgttaca ggaaaattaa gttgcaatta caaatatttc cgtttaatta taacaacaat
     7981 ctattgcaaa ttaaaatact atcaattacc atatggctta caacctaact aacgaaaggt
     8041 aggtaaagaa attgcaattt tttaactttt tgctttttta tcctgtattt atgtctattt
     8101 actggattgt cggttcaatt tatttctatt ttaccagaga aattagatat tcattgaaca
     8161 agaagcctga cataaatgtg gatgaattag aaggcattac atttttactt gcctgttata
     8221 acgaaagtga aacgattgaa gatacgttgt ctaatgttct tgcactcaaa tacgagaaga
     8281 aagaaattat tatcattaat gatggaagtt cagataatac agcagaactc atctataaaa
     8341 tcaaagaaaa taatgacttt attttcgtcg atttacaaga aaacagaggt aaagccaacg
     8401 cactcaatca aggcattaaa caggcttcat atgattatgt aatgtgcttg gatgcagata
     8461 ctatcgttga tcaagatgca ccatattata tgattgagaa tttcaaacat gatccaaaac
     8521 ttggtgcagt tacaggtaat cctagaattc gaaataagag ttctatttta ggtaaaattc
     8581 aaacgataga atatgcaagt ttaattggct gtattaagcg aagtcagaca cttgctggcg
     8641 cagtcaatac tatttcgggt gtcttcactc tatttaaaaa aagtgcagtt gtcgacgttg
     8701 gctactggga tactgatatg attaccgaag atattgcagt ttcttggaaa ttgcatttac
     8761 gtggatatcg tattaagtat gaaccgcttg ccatgtgttg gatgttggtt ccagaaacat
     8821 tgggaggtct ttggaagcaa cgcgtgagat gggctcaagg gggacacgaa gtattactac
     8881 gagacttttt tagcacaatg aaaacgaaaa ggtttccttt atatattttg atgtttgagc
     8941 aaatcatctc gattttatgg gtatatatag tgcttctata tttaggctat ttgttcataa
     9001 cagcaaactt cttagactat acatttatga catatagttt ttcaatattt ctactatcat
     9061 catttactat gacttttata aacgttattc aatttacagt cgcactcttt attgatagtc
     9121 gctacgagaa aaagaatatg gctggactca tatttgtaag ttggtatccg acagtatact
     9181 ggattattaa cgcagcagta gttcttgtcg catttccaaa agcattaaaa cgtaagaaag
     9241 gtggttacgc aacatggtca agcccagaca gagggaatac ccaacgctaa aatcatcgct
     9301 aaatattgta agagaaacag cacttatcgc tatatcttgt gtcttttgga tatattgttt
     9361 agttgttcta ctcgtttata ttggtactat atttgaaatt catgacgaaa gtatcaatac
     9421 aatacgtgtt gctttaaaca ttgaaaatac tgaaatttta gatatatttg aaactatggg
     9481 cattttcgcg attatcattt ttgtattttt tacaattagc atattgattc aaaaatggca
     9541 gagaggaaga gaatcgtgaa gtatagaaaa tttataattt tagtgttgag tatcttgatc
     9601 atattgcctg taagcacact ggatggtcat catattgcaa atgcagatga cgattcacct
     9661 aaaaaactga aatataaaga aaatagtgct ctggcattaa attatcaccg tgtaagaaaa
     9721 gcgaattttc tgaataattt tatttacttc ttttctagta gtaaagaaat taaaaattat
     9781 agtgttagtc aatcacaatt tgaatctcaa ataaaatggc taaaatcaca tgatgctaaa
     9841 tttttaacct tgaaagaatt tttatattac aagaaaaaag gtaagtttcc aaaacgaagt
     9901 gtatggatta actttgatga tatggatgaa actatttatg aaaatgctta tccaatctta
     9961 aaaaaatata aaataccggc aactgggttt attatcacag gtcatgttgg ggaagaaaac
    10021 tttcacaacc tcgatatgat tagtaaaaaa gaactaaaag aaatgtataa aactgggtta
    10081 tgggaatttg aaacacatac ccacgatttg cataacttat ctaaaaataa taagtcaaaa
    10141 ttaatgaaag cttctgaagc tacaatcata aaagatttaa acaaaagtga aaaatatcta
    10201 actaaaaact ttaaaaagtc gcagaaaact atagcctatc cttatggctt gatgaatgac
    10261 gataaattac cggtaatcaa aaaagctggg ttaaaatacg gtttttcatt agaggaaaaa
    10321 gcagtcactc cgaactccaa tgattattac atccctagaa tattaattag tgatgatgct
    10381 tttgagcatt taattaagag atgggacgga ttccatgaaa aagattagac ttgaactcgt
    10441 atatttacgt gctattatat gtgcaattat tattatcaca catttactta cacaaattac
    10501 tttaaaacat gaaaatatgg agggtggatc cttagtgtta caattttaca ttcgtaatat
    10561 tgtgattttt ggtacacctt gctttattat cttgtcacag ttactgacaa ccttgaatta
    10621 ccaaaaagtc acctatagat acttaactac acgcgtaaaa tatatactta ttccttacat
    10681 attaatggga ttgttttaca gttatagtga atcattatta acagattcaa gtttcaataa
    10741 acaattcatt gaaaatgtcc tattaggtca atggtatggc tattttatcg ttgttatcat
    10801 gcaattcttt attttgagtt atatcatttt taaaattaac tataacctat tcaacagtaa
    10861 aatattatta ttgttatctt ttattttaca gcaatcattt ttatattact ttacgaacaa
    10921 cacagcgttt cacgataccg tgctacacta ttatccatta agtgaaaata ctataatatt
    10981 cggatggatt ttttatttct tcttaggtgc atatatgggt tataactacg aacgtgtatt
    11041 aaatttctta gaacgttatt tagttattat gattgtatta gctgtagcta cttattttgt
    11101 gtttattgcg ttagcaaatg gagactattg gaacgttacc agcttttcat attcattaac
    11161 accatataat agtattatgt ttattgttat cttgggtatt tgcacgcatt ttaaaacaat
    11221 gttatttaat acgattcaaa tgattagtgc tttctcattc tttatttatt tattacatcc
    11281 aatcattcta gactcattgt ttgcatatac aaatatattt gaggataata caatggtctt
    11341 tctagcgata tcactactat tcattttagg attatgtata ggtgtcggca tgatattgcg
    11401 tgaattctat atctttaggt ttattattgg aaaacaacca tataaattga acattaatgc
    11461 ttattaatta ttaagctatg ttaaaaacac gcggtgggcg aaatcagttt gaattgactg
    11521 acttcgtttt accgcgtgtt taatattgtt atacatatat tctaattgca catttaaact
    11581 tcgtaaatgc caatgggagt gggacagaaa tgatattttc gcaaaattta tttcgtcgtc
    11641 ccaccccaac ttgcacatta ttgtaacctg actttccgcc agcttctatg ttggggcccc
    11701 gccaacttgc acattattgt aagctgactt tccgccagct tctttgttgg ggccccgcca
    11761 acttgcattg tttgtagaat ttcttttcga aattctttat gttggggcct cgccaatgtt
    11821 ttacttgaat aattctttta gaattctaaa taatgatccg attaattgaa agaagtctgc
    11881 agtcattatt aattcctccc tttactttat aaattatgct tgcttagtat cagtcagctt
    11941 ttcagttttc actaaatcgt ctgctaaatg atgccaaaaa tcttgtaatt cttctcttgt
    12001 gcgcactgta tcagaactgt cttgtcctac aaagtcaaca tgatcccaat catgttttgt
    12061 aggcgtcact tgccaaatgc ctttttgaat tttatctgtc gcttttgtat aagcttgatt
    12121 aaatggatgt tgagaagaaa taacggatac taaaccatcg ttttctcgcc attctttttc
    12181 agtagcttta ccgattaagt taccagtaat cacaaatggg aaaaacatat ttaagtctgc
    12241 tttttgtcta tcgctattta atgctttgtg cgttgcttca ccagtgtatg ttttatacac
    12301 aatgttaggg ttcaacgacg ttttacgatt taaatctgtt gcaccctcac gcgtcagatc
    12361 gtaaaatcca ttatcttttg atttccataa attagattgt ttaacgcgtt tgacataatc
    12421 aatatatgat tcatttggct tctgttttag accccattga gccaacccga agtctactct
    12481 tgaattttta ttaccaaaca ttttaccgat atcaaatacg atttgtctca ctaaagcttc
    12541 attaccagct aaatctgatg cgtgtgtacc attatgtggt gttcctaaag tagtaattga
    12601 tgaaatcata ttgtcatgat tacctttgaa tagtggagaa atttcgccac catgtttctt
    12661 ttgatactct atttcttcac gattaccatt acgcagtaat tcttctagtt gacgtatcgt
    12721 ttgaccgccc atactatgtc caactaggtg taccttctgt cctggtttcc agtctttgta
    12781 aattccttcg tatgtttttc cataacgttc atgtccatat tttgctgcat gtgctgcacc
    12841 ataatctaca cgaccgcctt tgatataata ataaagttca actgcgcggt catagttact
    12901 tccaaaagca cttatacttg cttcataagc tttgtaacca ttttcttcta aatcttggcg
    12961 aatgttcatt ttattaccgc cccaataatg agctaacact gaaggattaa tatcatctgt
    13021 aaacccattg aaaccatgca ctaaaacgat aggatcctga tttttatact gcccttgttt
    13081 ggcaacttta ttcgtttgat catctttcgc ttttgcagta agttcctttg tccctttgtt
    13141 tggtaacgtt gctactgcac tactttttaa taaatttaat ccatttttct tgtcattgtc
    13201 ctttgttggt tctactggtg atttttgatt gttttttaaa tctgaaactg atagtgtttt
    13261 ttcgtctttt tgaggtttca cttcttgttc attgttaaaa ttgtagttag taattttatc
    13321 attcgcttga ttattttgtt gtggttgtac tttctctatt tctgtctcat taacaatttc
    13381 tcgttgactt gtttgatcta acttatttaa tgatggtgct tcctttattc gactttgtga
    13441 aggtagattg ttattagcgt ttgaatcttg actattatct gcctcttttg actgttgctg
    13501 tgacgccact ttactttgtt taccatcatt aatagcctgt tctaaccctt gatttacatc
    13561 gctaggatta gcattgttat tttttgttgt atcacgttga atatttttat tattaagcga
    13621 tgatttttta tcatccttta gttgatcttt attcaaatca tctgttttac gatgattttc
    13681 ttctgatatt gttttatcat tatgcaagtt cttagttaaa ttttcagtat gttgctgttc
    13741 gtcaacaccg ttttcacgtt gataattagc attttcccta gtttgttgat ccccaataga
    13801 ttgtgctgta actgtatcct cctgtgaatt tcccatattc acttgcttct cagctgcttg
    13861 tgcttgtcca ccacttaaaa atagtaatgt agctattaaa atggaagatg cacctacact
    13921 aaatttacga atactatact tattttgact tttcatcatt gtcagcacct ctattttttt
    13981 attttagata aacaaattga taattattcg aggaataaac tcaaataatt tacttaacca
    14041 agcactcata atgaccatcc tttctattgt ttaatttgac gagaaagcgt ttacatttgt
    14101 atttaaaaaa tatattctcc aaa
//
LOCUS       DALAAR010000062        13964 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000062 DALAAR010000000
VERSION     DALAAR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.064"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            129..2234
                     /gene="clpL"
                     /locus_tag="O2458_002033"
     CDS             129..2234
                     /gene="clpL"
                     /locus_tag="O2458_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCZ3887889.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2303..2473)
                     /locus_tag="O2458_002034"
     CDS             complement(2303..2473)
                     /locus_tag="O2458_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCZ3887890.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2486..4480)
                     /gene="feoB"
                     /locus_tag="O2458_002035"
     CDS             complement(2486..4480)
                     /gene="feoB"
                     /locus_tag="O2458_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCZ3887891.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4492..4719)
                     /locus_tag="O2458_002036"
     CDS             complement(4492..4719)
                     /locus_tag="O2458_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCZ3887892.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4935..7424)
                     /gene="farE"
                     /locus_tag="O2458_002037"
     CDS             complement(4935..7424)
                     /gene="farE"
                     /locus_tag="O2458_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCZ3887893.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7569..8117
                     /gene="farR"
                     /locus_tag="O2458_002038"
     CDS             7569..8117
                     /gene="farR"
                     /locus_tag="O2458_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCZ3887894.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8194..8361)
                     /locus_tag="O2458_002039"
     CDS             complement(8194..8361)
                     /locus_tag="O2458_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887895.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(8473..10017)
                     /gene="pruA"
                     /locus_tag="O2458_002040"
     CDS             complement(8473..10017)
                     /gene="pruA"
                     /locus_tag="O2458_002040"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCZ3887896.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10207..10806)
                     /locus_tag="O2458_002041"
     CDS             complement(10207..10806)
                     /locus_tag="O2458_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCZ3887897.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11045..11236
                     /gene="cwrA"
                     /locus_tag="O2458_002042"
     CDS             11045..11236
                     /gene="cwrA"
                     /locus_tag="O2458_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCZ3887898.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11476..13884
                     /locus_tag="O2458_002043"
     CDS             11476..13884
                     /locus_tag="O2458_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCZ3887899.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         4600 a         2569 c         2238 g         4557 t
ORIGIN      
        1 ttaaaaatat tctgttttat cacctactat tagtggaaaa gtacaattgc aattgtatat
       61 agtttgcata acgcttcaaa agtaatttct tttttgttta gttcaaaaaa atttagaggt
      121 gatgttatat gaataacggt tttttcaata gcgactttga ttcaattttt cgaagaatga
      181 tgaaagatat gcaaggttca aatcaagtcg gaaacaaaaa gtactatatt aatggtaaag
      241 aagtttcacc tgaagaacta gcgcaactca cacaacaagg tggcaatcac tctgctgaac
      301 aaagtgcgca agcttttcaa caagcagcac aaagacaaca agggcaacaa ggtggcaacg
      361 gcaattattt agaacaaatt ggtcgtaacc ttacgcaaga agcacgtgac ggtttattag
      421 atccagtcat tggtcgtgat aaagaaattc aagaaactgc tgaagtttta agtagacgaa
      481 ctaaaaacaa tcctatatta gttggagaag ctggtgttgg taaaactgcg attgttgaag
      541 gtttagcaca ggcaatcgtt gaaggaaatg taccagcagc aatcaaagac aaagaaatta
      601 tttctgtaga catttcatca ttagaagctg gaacgcaata tcgtggtgct tttgaagaaa
      661 atattcaaaa attaatcgaa ggtgttaaat cttcacaaaa tgccgtacta ttctttgatg
      721 aaatccatca aattatcggt tcaggtgcca caggaagtga ttcaggtagc aaagggttat
      781 ctgatatttt gaaacctgca ttaagtcgtg gtgagatttc tattattggt gcaacaacac
      841 aagatgaata tcgaaacaat attcttaaag atgctgcatt aacgcgcaga tttaatgaag
      901 tgcttgttaa tgaaccaagc gctaaagata ctgttgaaat tttaaaaggt attcgcgaaa
      961 aattcgaaga acaccatcaa gtaaaattac cagatgacgt attaaaagca tgtgttgact
     1021 tatcaattca atatattcca caacgattat taccagataa agcaatcgat gtgttagata
     1081 ttacagcagc acatttatct gcgcaaagtc cagctgtcga taaagttgaa actgaaaaac
     1141 gaatttctga attagaaaat gataaacgta aagcagtaag tgctgaagaa tataaaaaag
     1201 ctgacgacat tcaaaatgaa atcaaatcat tacaagataa attagaaaat agtaatggtg
     1261 aacatactgc tgttgctaca gttcatgata tttcagatac tattcaacga ttaactggta
     1321 ttccagtttc tcaaatggat gataacgata ttgaacgttt aaaaaatatt tctaatcgtt
     1381 taagaagtaa aatcataggt caagatcaag ctgtagaaat ggtttcacgt gcaattcgcc
     1441 gtaatcgtgc tgggtttgat gacggcaacc gtccaattgg cagtttccta tttgttggcc
     1501 ctactggtgt tggtaaaaca gagcttgcta aacaattagc aattgattta tttggtaata
     1561 aagatgcact gattcgactt gatatgagtg aatatagtga cacaacagct gtttcaaaaa
     1621 tgattggtac aactgctggt tatgttggtt atgatgacaa ttcaaatacg ttaactgaaa
     1681 aagtacgccg taatccatac tcagtcattc tatttgatga aatcgaaaaa gcaaatccac
     1741 aaattttaac attgttatta caagtaatgg atgatggtaa tttgactgat ggtcaaggta
     1801 atgtcatcaa ctttaaaaat acaattatta tttgtacatc aaatgctggc tttggcaatg
     1861 gcaatgacgc tgaagaaaaa gatattatgc acgaaatgaa aaaattcttc cgccctgaat
     1921 tccttaaccg cttcaacggc atcgttgaat tcttacattt agataaagat gcattgcaag
     1981 atatcgtcaa cttattatta gacgatgtac aagttacatt agacaaaaaa ggtattacga
     2041 tggacgtttc tcaagatgcg aaagattggt taattgaaga aggctatgat gaagaattag
     2101 gtgcacgtcc attaagacgt attgttgaac agcaagtacg tgacaaaatt acagattact
     2161 atttagatca tacagacgtt aaacatgtgg atatagatgt tgaggataac gaattagtcg
     2221 taaaaggtaa ataacgacac tttaacatat cgcgcatcaa aaatgagcat caggtcgccc
     2281 ttgcctgtgc tcattttttt aattatttcc ctggaaaatg attcgctgtg tgctgttctg
     2341 ttccacaaca atcacgatta atgtcacatg taccacattt tccttgtttt gaacgcttga
     2401 aaaattttac tagtgtatat aaggcatatc cgaaaattgc taaaaaaatt aaaatgttaa
     2461 taatgactga cactttaacc actccttaaa caaataaatg tccgacttga taaaaaatga
     2521 atgttaagac atatgcagtg actagaggat aggcaactgc aagtgccgtc catttccatg
     2581 aataagtctc tttacggatt gctgctactg tagaaacaca aggaatatac aatagtataa
     2641 atatcataaa tgcatacgca gatagcggtg tgaattgatt ttgaatcaca ttaacaaggc
     2701 ctgcatcacc tgatgaatag ataatcgcca tcgaacttac gataacttct tttgctaaaa
     2761 atcctggcac taacgtagca cctgcttgcc atgttccaaa tccgagcggt tgcactaaca
     2821 taccaaagaa actaccaacc atatgtaaaa aactttgatt gatattcaca ttgataccat
     2881 gtggtcctac ataacttaat agccaaatga ctactgagcc gccaaaaata aatgtacctg
     2941 ctttacgaac aaagccctta gccttttccc aagtactacg ccacaacgtt ttaatggaag
     3001 gcacacggta tgttggcaat tccacaataa agattgcatt atcatttttt aaaatcgtct
     3061 tagtaagtac tgtactgact aaaaatgcca taataatacc taaaacatac aggcttaata
     3121 ctactaaaga ttgattctct ttgaaaaaga tacctacgaa caacgcatac actggcagtc
     3181 tagcagagca tgacatgaat ggtgcaatta atatcgttgt taaacgctct ttttcatttt
     3241 caatactgcg cgcagccata atactcggta cattacaacc aaatccgata atcattggta
     3301 taaaagactt cccgcttaaa ccgaacgatt ccataatacg atccattaaa accgcaatac
     3361 gtgccatata acccgaatct tctaataaag atataaagaa aaagagcaca acaatttgtg
     3421 gtacaaagac taatactgat cctacaccag caataatgcc atctgtaatt aaatcttgta
     3481 aaaatggtat aacaccaaga taattcataa tcgtcttcac actatctgta aatgtaccac
     3541 ctataaatgc atcgagttga tccgacaaag gtgtgccaat ccatgtaaat gtagtttgaa
     3601 agatcaacca cataattgct agaaagatag gcatccctat atatttatgt gttaatatct
     3661 tgtctattct agagctgaaa tattgcttat cttcatctgg atacgttacc acgtcttgca
     3721 ataacgtctc aatataatga ttgcgtatac gctccatctc tcgacgaaca gatacagccc
     3781 ctacttgttc agcaacttga tcacgtaaac tcgacaattt atttacaacc tctgaattaa
     3841 gttcgtttgc aatttgcatg ttatttaata agaattgaat cgcaataaac ctagcttgat
     3901 acttatcatg agatgtctct gtcattatta tttgacacat atttttaatt gtctcttcaa
     3961 tcttctcacc ataattgatt ttaaaatgcg gttgataccc ttcccctaga tgctttattt
     4021 cgccaagtaa atattttgtt cctttgcctg tacgtgccac aactggaaaa ataggtgttt
     4081 ttaacttttt catcaattta tgataatcga tttttatccc gcgctttgta gctacatcaa
     4141 tcatatttaa tccgatgtat attggttgat taagttctaa caattgtact gttaattgca
     4201 tatttctttt tagttgactc gcatcaacaa tgttaatgat tcctgaaaat gaatcgttta
     4261 ataaatagtc tgtcactaca gtttcatctt tagaaatcgg cgataaatca tatgtacctg
     4321 gtaaatcaat taattgtcct acattttctt taagtttccc tacttttttc tctaccgtta
     4381 cgccactcca gttgcctata tattcatacg aaccagttaa agcgttaaac aaagatgttt
     4441 taccaacatt aggatttcct aaaatacaat aattttccat tcgtccggct cctattcttc
     4501 taatgcaata gaacaagcat cgcaatgtct aatacttaac tgttgtccgt ttacttcaat
     4561 aatacatggc cctttaaata aacatttttg tttaatcgtt atgatagcgt catctgttaa
     4621 cccaaaggca cttagacgat acaacatatt ctcattagca atatccattc gctttatttt
     4681 ataagcctta ttcatttcac catttttaat gtttaacata ctatttcgct ctcctattag
     4741 aaataataat cattatcact taaaaatcat aacccttaaa attgtagctc gcaatacttt
     4801 atttaaataa ttttcatttt tcatgtaaaa tttgtgacat tgcaaaaatg ttttgtataa
     4861 tcacttccat gcaaaaaacc tccgcttcaa atgtcattga tagacattta aagcggaggc
     4921 atgtacatgt tattttatga tttatttcgt agattttttt ctaacaattg attgagcttt
     4981 tcaatatttt tattcaactg ttctatattc gttgcttgtt tttttgaatt tagttgatgc
     5041 ttgttttgat aatctattaa agcatcatat aagaacaatg aacttgctga ttgctgtttc
     5101 acttcttggt acaatgccat cgttttatca tctagtacta ttgaatgtgc atcatcttgc
     5161 ccatgtgcat cttgatgttt cgatacattt gttgcacctt gtggatatac tttaaaggtt
     5221 gtgtcgtatg tttccttact atctttagct tcagattcct gtgattcaac cgttttatat
     5281 ttttcaagtg catgtccttc aatatcaact cgtggaataa tgcgattcaa ccatgctggt
     5341 aaataccacg aaccttttcc aaacaatttc gttaatgcag gaattaacat cattctgact
     5401 acgaaggcat caaagagtac accaaacgct aatgccatac ccattgattt aatcatgaca
     5461 tcttcttgga atacaaacgc aaagaagaca ctaaacataa ttaatgcagc tgctacaata
     5521 acaggaccgc tttctttcaa tcctactttg atagaataat cattatcccc tgttttacta
     5581 tattcttcat gaattcgcga cataaggaag acttcataat ccatcgctaa tccaaataag
     5641 atacctatag taataaccgg taaaaatgct agcattggtc ctgtcgtttc aataccaaac
     5701 agacctttca taaaaccatc ttgcattact aatgttgtaa atcctaatgt tgccattaat
     5761 gacaagacga atcctaaaac tgcttttaat ggtattagaa ttgaacggaa gacaatcatt
     5821 aataagaaaa atgctaatac aacaatgact gaggcaaata aaggtatcgc ctcatttaac
     5881 tttttagaca tatcaatatt aatgacactt tgtcccgaaa tctccgtttt gaacccatat
     5941 ttatcttgtg catctttatg ataatctcgt aaatcatgca ctaaatcatt tgtactctct
     6001 gcattaggcc cttgcttagg tatcacgacc atcaaagcgt aatcattatc tttactcatt
     6061 tgtggtggcg taacgatatc tacatttttc ttatctttaa tatctttata tacagactgt
     6121 aaatcttgtt gtaatccttg tggatcatcc tttttatctt tcacatttat caacatcggt
     6181 atttggccat taaatccttc accaaattta tccgagataa tatcgtaagc ttttttctgt
     6241 gtagaatctg ctggtttaac accgtcatct ggaataccaa gtcgcatatg actaactggt
     6301 attgcagctg ctactaatat gattaaacct agtaatactg ccgcaagtgc atttcctgta
     6361 ataaatttag accatggcgt atcaatatct tttttgaatt tagactgtaa tttattcact
     6421 ttaatgcgtt tatggaaaat gcttattaat gcaggtaata aagttaaagc gctaagtact
     6481 gcaaaaacaa cactaattgc cgaagcaaat cccattaccg ctaagaagtc aatgcctact
     6541 aatgataaac cacatactgc aattacaact gttacaccag caaaaacaac tgcactacct
     6601 gctgttccta ttgcaagacc aatgccttta atgtaatctg tttcagtttt cataacttgt
     6661 cgatatctga ataaaataaa taatgcataa tcgataccaa ctgctagtcc aatcattacg
     6721 gctaatgtca gtgtgacatt tggtatatcg aatgcataag ttaacaaact gataatacct
     6781 acaccagagg ctagaccaat caatgcactt ataattggta atcctgcagc aatgactgaa
     6841 ccgaatgtga ttaacagtac aacaaatgca acaataatac caactagttc agaattaccg
     6901 cctacttctg tagatgtcat tcctgtacca gttaactctg tttgtacatt atgattgtca
     6961 cgtaagtctt tcaaatgact tttaacatta tctctagagc catcttttaa agatgtttga
     7021 ctaacgtcat atgtgatatc tgcaaatgca gttgttttat ctttactaat ttgcttattt
     7081 tcataaggat ctgatatttt atcaatgtgc ttgtcatctt ttttaatatc atctaacgtt
     7141 ttcttaatat ctttagtaat gttcggttgc acaataccat catctttagt cgtcttaaag
     7201 acaacacgta tttgtgcctt ttcactatct tgattaaaat gtttttcaat ctttttattc
     7261 gtatctaacg actctaatcc tgtcatttta atatcattgt caaatttcgg tgcatttgta
     7321 gcaagtggta tcaatattgc agctacaatc actatccatg caatgaccgc ggaccattta
     7381 tgttttgcga tgaatgtccc catcttatat aaaaattttg ccaaagtata ttgcctcctt
     7441 ttaaaatcaa cgttatagtt taaatataca gtgtagatta ttgttcgatt atagtatcta
     7501 tccccgacct cttaaagaat caattggaaa attttgtata ttaaactaca cacaaaggag
     7561 aaatgtagat gaaagagact gatttacgag ttataaagac aaaaaaagca ttgtcgagta
     7621 gcttgctaca attgttagaa cagcaattat tccaaacgat tactgtcaat caaatttgcg
     7681 acaacgcact cgtacaccgt acaacatttt ataaacattt ttatgataaa tatgatcttc
     7741 tagagtactt gttcaatcaa ttgactaaag actactttgc tagagatatc agtgaccgtc
     7801 ttaatcatcc attccaaacg atgagtgata cgattaataa taaagaggat ttgagagaaa
     7861 tcgcagaatt ccaagaagaa gacgctgaat ttaataaagt attaaaaaat gtctgcatta
     7921 aaattatgca taacgatatc aaaaataata gagaccgtat cgatattgac agcgacatcc
     7981 cagataatct catattttat atttatgact cgttgattga aggttttata cattggataa
     8041 aagatgaaaa aattgattgg cctggcgaag atattgataa cattttccat agattaatca
     8101 atattaagat taaatagtag atgagaaact catgagcgtt accaacattc ataataaaaa
     8161 cgatagtgta cacgttaatg aattcgtgta ctactatcgt tttttatttt tatcgtgctt
     8221 atcgctatta aaacaactga tacacaacac ataaactatg aagaaaaaaa taaatccgct
     8281 atctaaatga ctttgactca gttgtttaaa tgaccaaatt gctaatacaa ttcccattat
     8341 tattgaaata acgtatctca cattcttata cctataatcc ttttctaaaa atatggttgc
     8401 tattacttaa tttttaaagt tataaataaa aagagccaac cgcaatggat ggcccttgtt
     8461 cattatgaag cattagaaca tttctgaaac aaccttttgt tctaagaagt gtaataagta
     8521 gtctggacta cctgttttag cgtccgtacc tgacattttg aaaccaccaa atggatggta
     8581 tccaacaact gctgaagtac agcctctgtt aaggtataaa ttgcctacat caaattcgtt
     8641 taccgcttta atccaatgct cgcgattatt tgtaatcact gcaccagtta aaccgtaatc
     8701 tgtatcattt gcaacctcaa ttgcttcatc aaaatcgtta actttcacaa agccaacaac
     8761 tggaccaaaa atttcttctt gcatgattct atctttagat ttaagtcctg aaatgattgt
     8821 tggttctaca aagtaacctt ttgaatcatc agtgccgcca ccttgttcta atttaccttc
     8881 ttctttacca atctcaatat aatttttaat cttatcaaat tgttttttat taataactgg
     8941 gcccatatac gtattgtcta cagtattgcc caacgttaat tcttttgtta atttgattga
     9001 tttctctaat acttcgtcat aaacgtcttt atgcacaatt gcacgtgaac atgctgaaca
     9061 tttttgacca gaaaaaccaa atgctgacgt tacaatagct tctgctgcca tatctgtatc
     9121 aatattttca tcaactacaa tggcatcttt accacccatt tcagcgataa cacgtttcaa
     9181 gaagttttga ccttcttgaa caacggcact acgttcataa attctagtac ctgtcgcacg
     9241 tgatcctgta aatgtaacga aatgcgtatc tttatgatca actaagtaat caccaatttc
     9301 tttcggatca ccaggaacaa agttaactac gccttttggt aatcctgctt cttctaaaat
     9361 ttccattaat ttataagcga tataaggtgt atcctcagca ggtttcaata acactgtatt
     9421 acctgccaca actggtgcta aagttgtacc agccataatc gcaaacggga agttccacgg
     9481 cggaattgta acacctgtac caattgattt atagaaatat ttattgtgtt caccttcacg
     9541 atcaagtact ggcttacctt gagccaagtc catcattgaa cgtgcatagt attcaataaa
     9601 atcaatacct tcagctgcat caccaactgc ttcatcccat ggcttacctg cttcataaac
     9661 cataattgct gcaatttccg cttttcgacg acgaataatt gccgaaacac gtaacataag
     9721 ctctgcacga tcatttgctg accatgtttt ccaagattta taagcttcgt ttgctgcttt
     9781 aaacgcatct tcaacatctt gttttgttgc ctttgatgca tttgcaatca cttgtgatgt
     9841 gtctgcagga ttgattgatt taattttgtc atctttgaaa atcttctctc cattaatcac
     9901 taatggtatg tcttgaccta attctttttc cacgtctttc aatgctttct taaacatatc
     9961 cacattttct tggactgaaa aatcgtaacc aggttcattt ttaaattcta ctaccatgta
    10021 cacttacccc ctataaattt tgaaagtggt ttaacccttt gatttaatga tataacatca
    10081 tttaaactca ttttactatg attaaggtta gttttgcaat cgctttcatt tttatgtttt
    10141 atcacttatt ctcaagtatt ttgaaattga ttggttactt tttaaaattt atatgggtcg
    10201 caactgctac tttatcgttt cgtcatttaa tgtttcggat ggtaggtcat tatcaatttt
    10261 acgaacgact ttacaagggt ttccaaccgc taagctgtgt ggcggaatat ctttagtgac
    10321 aacactacca gcaccaatca cactgccttc tccaatcgtc acccctggta acacggctac
    10381 atgaccgcca aaccaagtat tactgccaat atgaatgggt ccggcttttt caaaaccttc
    10441 atttctatga tggaaattaa gtggatgtgt cgctgtgtag aatccacaat taggtcctat
    10501 aaaaacatta tcgccaattg taatctgtcc accatccata aagtaacaat tggtgtttac
    10561 atagacattt tttcctagtt taacgttcca accataatct gtatcaaaag gaatcgaaat
    10621 acttacattg tctgttgttg tttgaaataa ttgatcaatt aattcctttc ttttatttgt
    10681 agcactcggt cttgtatgat ttaattcaaa gcaaatatct ttcgctcgtg cacgttcatt
    10741 gattaagtat tgatcaaagt ttgcatcgta ccatttttct gctaacattt tttctttttc
    10801 agtcattaca cctttcaact cctaataact tatttacttg tttaaaagtt aatcaaataa
    10861 accttcgcct atgcaactaa tacgctataa cattatgaaa tcatgacctt atcaccctta
    10921 tctatacaat tctcgcatca aatactgcta aagtagtaga taaattcaat actacagacg
    10981 cattcatttt ttaatctatt aacgtacaat gtgagtaaga gaaatataaa ggagtatgat
    11041 agcgatgaga atattaatta caggcacagt tgctatctta atcattctag gtttggtcaa
    11101 aacgatacaa gattacgaaa tgacaaacga cacgagtcgt cagttgtcag acaacaaaga
    11161 tgatgataaa gtcatccatc ttaataattt taaaaattta catgcgaaag aatttaaccc
    11221 atctgatttc ttttaagtca cctaagaatt gcaaatccag aagtcattta agttttacct
    11281 ttcattcata catcctttaa tattaattac gacttctttt atatagatgc taagtagaga
    11341 gattgttgtg caatgtttgc acggcaatct ctctttttct ttttaaaatt ggtaaaagta
    11401 aaacgcaacg attgacttat atacctatag ggggtacatt agacgtgtaa caatgaatca
    11461 cagggaggca ataatgtggc taatacgaaa aaaacaacat tagatatcac tggtatgact
    11521 tgtgccgcat gttcaaatcg tatcgaaaag aaactgaata aacttgatga cgttaatgcc
    11581 caagtgaatt taactacaga gaaagcaact gttgagtata accctgatca acatgatgtc
    11641 caagaattta ttaatacgat tcaacattta ggttacggtg tcgctgtaga aactgtcgaa
    11701 ttagacatta caggtatgac ttgtgctgca tgctcaagcc gtattgaaaa agtgttaaat
    11761 aaaatggacg gcgttcaaaa tgcaacggtc aatttaacaa cagagcaagc taaagttgac
    11821 tattatcctg aagaaacaga tgctgataaa cttgtcactc gcattcaaaa attaggttat
    11881 gacgcgtcta ttaaagataa caataaagat caaacgtcac gcaaagctga agcgctacaa
    11941 cataaattga ttaagcttat catatcagca gtattatctt taccactatt aatgttaatg
    12001 tttgtacatc ttttcaatat gcatatacca gcactattta cgaatccatg gttccaattt
    12061 attttagcta cacctgtaca atttattatt ggatggcaat tttatgtagg tgcttataaa
    12121 aacttaagaa atggtggcgc caatatggat gtacttgttg ctgttggtac aagtgcagca
    12181 tatttttaca gtatttatga aatggttcgt tggctaaatg gctcaacaac gcaaccgcat
    12241 ttatactttg aaacaagcgc cgtactaatt accttaatct tattcggtaa gtatttagaa
    12301 gctagagcga agtctcaaac aaccaatgcg cttggcgaat tattaagttt acaagctaaa
    12361 gaagcacgca ttttaaaaga tggtaatgaa gtgatgattc ctctaaatga agtacatgtt
    12421 ggagatacac ttatcgttaa accaggtgaa aagatacctg ttgatggcaa aattattaaa
    12481 ggtatgactg ccatcgacga atctatgtta acaggtgaat ctatccctgt tgagaagaat
    12541 gttgatgata ctgtaattgg ttcaacgatg aacaaaaacg gtactattac tatgacagca
    12601 acaaaagttg gcggggacac tgcgttggca aatattatta aagttgtcga agaagctcaa
    12661 agttctaaag cgccgattca acgattggca gatattattt ctggttattt cgttcctatc
    12721 gttgttggta tcgcactatt aacatttatc gtgtggatta ctttagttac accaggtaca
    12781 tttgaacctg cacttgttgc gagtatttcc gttctcgtca ttgcttgtcc atgcgcattg
    12841 ggacttgcta caccaacttc tattatggta ggtactggtc gcgctgctga aaatggtatt
    12901 ttatttaaag gtggcgagtt tgttgaacgc acacatcaaa ttgataccat cgttttagat
    12961 aagacgggta ccattacaaa tggtcgtcca gtcgtgacag attatcatgg tgacaatcaa
    13021 acgctacaac tacttgctac tgctgaaaaa gattctgaac acccattggc agaagccatt
    13081 gtcaattatg caaaagaaaa gcaattaata ttaactgaga caacaacatt taaagcagta
    13141 cctggccatg gtattgaagc aacgattgat catcactata tattggttgg taaccgtaaa
    13201 ttaatggctg acaatgatat tagcttgcct aagcatattt ctgatgattt aacacattat
    13261 gaacgagatg gtaaaactgc tatgctcatt gctgttaatt attcattaac tggtatcatc
    13321 gcagtggcag atactgtcaa agatcatgcc aaagatgcta taaaacaatt gcatgatatg
    13381 ggcattgaag ttgccatgtt aactggcgat aataaaaaca ctgctcaagc cattgcaaaa
    13441 caagtaggca tagatactgt tattgcagat attttaccag aagaaaaagc tgcacaaatt
    13501 gcgaaactac agcaacaagg taagaaggtt gcgatggttg gtgacggtgt aaatgatgca
    13561 cctgcattag ttaaagctga tatcggtatc gccattggta caggtacaga agttgccatt
    13621 gaagcagctg atattactat tcttggtggc gacttgatgc ttattcctaa agccatttat
    13681 gcaagtaaag caaccattcg taatattcgt caaaatctat tttgggcatt cggctataat
    13741 attgccggta tccctatagc tgcattgggc ttacttgcgc catgggttgc tggtgctgca
    13801 atggcactaa gttcagtaag tgttgtcaca aacgcactta gattgaaaaa gatgcgatta
    13861 gaaccacgcc gtaaagatgc ctagattcct taataatgaa ggattcgttg gtgattctga
    13921 gataggctag tgattggctc tataatgtcg cggtttacag ttgg
//
LOCUS       DALAAR010000063        13618 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000063 DALAAR010000000
VERSION     DALAAR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.065"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            61..348
                     /locus_tag="O2458_002044"
     CDS             61..348
                     /locus_tag="O2458_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887900.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            354..1835
                     /locus_tag="O2458_002045"
     CDS             354..1835
                     /locus_tag="O2458_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ3887901.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1924..2280)
                     /locus_tag="O2458_002046"
     CDS             complement(1924..2280)
                     /locus_tag="O2458_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ3887902.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2757..3716
                     /locus_tag="O2458_002047"
     CDS             2757..3716
                     /locus_tag="O2458_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3887903.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3762..3893)
                     /locus_tag="O2458_002048"
     CDS             complement(3762..3893)
                     /locus_tag="O2458_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887904.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3957..4172)
                     /locus_tag="O2458_002049"
     CDS             complement(3957..4172)
                     /locus_tag="O2458_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ3887905.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4337..4888
                     /locus_tag="O2458_002050"
     CDS             4337..4888
                     /locus_tag="O2458_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3887906.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4940..5878)
                     /locus_tag="O2458_002051"
     CDS             complement(4940..5878)
                     /locus_tag="O2458_002051"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCZ3887907.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            6060..7421
                     /locus_tag="O2458_002052"
     CDS             6060..7421
                     /locus_tag="O2458_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCZ3887908.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            7408..9081
                     /gene="menD"
                     /locus_tag="O2458_002053"
     CDS             7408..9081
                     /gene="menD"
                     /locus_tag="O2458_002053"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCZ3887909.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            9068..9871
                     /gene="menH"
                     /locus_tag="O2458_002054"
     CDS             9068..9871
                     /gene="menH"
                     /locus_tag="O2458_002054"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCZ3887910.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9864..10685
                     /gene="menB"
                     /locus_tag="O2458_002055"
     CDS             9864..10685
                     /gene="menB"
                     /locus_tag="O2458_002055"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCZ3887911.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(10924..11253)
                     /gene="sspC"
                     /locus_tag="O2458_002056"
     CDS             complement(10924..11253)
                     /gene="sspC"
                     /locus_tag="O2458_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCZ3887912.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(11291..12472)
                     /gene="sspB"
                     /locus_tag="O2458_002057"
     CDS             complement(11291..12472)
                     /gene="sspB"
                     /locus_tag="O2458_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCZ3887913.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(12554..13564)
                     /gene="sspA"
                     /locus_tag="O2458_002058"
     CDS             complement(12554..13564)
                     /gene="sspA"
                     /locus_tag="O2458_002058"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCZ3887914.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
BASE COUNT         4879 a         1876 c         2444 g         4419 t
ORIGIN      
        1 aatagcgttg tgaagtataa aattattttg taaaacaatt ttgttattaa gaggtgcgct
       61 atggaaaagg ggattttcaa ttacgataat gcgaatgtat taaaattaga tactaatcag
      121 ttaaatgaaa acataaaagt cattgacgat atatttaaaa attatgagca gatagagcca
      181 actatagaag tcgaaaatgg aaatacaaaa ttaaaattaa atggatattt tattgcgtca
      241 attataagcc cattaaattt aaataaactt aataatttat atgttgaaga agaattttat
      301 catacatata acgaattaat tgttaaatat actgaggtaa aggaataatt ataatgaaaa
      361 aaatatttat gatggtacat gagttagatg tgaataaagg tggtatgacc tcttcgatgt
      421 tcaatagaag taaagagttt tatgatgcgg acatacctgc tgatattgtt actttcgatt
      481 acaaaggaaa ctatgatgaa attattaaag ctttgaaaaa acaaggtaaa atggatcgaa
      541 gaacgaaaat gtataatgta tttgagtatt ttaaacaaat ttcaaataat aaacatttta
      601 agtctaataa attgttatat aaacatattt cagaaagact aaaaaatacg attgaaattg
      661 aagagagtaa aggtatttca agatattttg atataacgac tggtacatat attgcctaca
      721 ttagaaaaag taaatctgaa aaagtgattg atttctttaa agataataaa cgaattgaac
      781 ggtttagttt tatagataat aaagtgcata tgaaggaaac atttaatgta gataataaag
      841 tttgttatca agtattttat gatgaaaagg gatacccata tatttcaagg aatattaatg
      901 ctaataatgg tgctgtaggt aaaacttatg tgttagttaa taaaaaagaa tttaaaaaca
      961 atttagcact gtgtgtttac tatttagaaa aactaataaa agattctaaa gatagtatta
     1021 tgatttgtga tggaccaggg agttttccaa aaatgtttaa tacaaatcat aaaaatgctc
     1081 agaaatatgg cgttattcat gttaatcatc atgaaaattt cgatgatacg ggtgcattta
     1141 aaaaaagtga gaaatatatt attgagaatg cgaataaaat taacggtgta attgtattaa
     1201 cagaggcaca aagattagat attcttaatc aatttgatgt agaaaatatt ttcactatta
     1261 gcaattttgt taagatacat aatgctccaa aacattttca aactgaaaaa atcgtaggtc
     1321 atatttctag aatggtacca acgaagcgaa ttgatttgct tattgaagtg gctgagttag
     1381 tcgtaaaaaa agataatgct gttaaatttc atatatatgg agaaggatct gtcaaagata
     1441 aaatagctaa aatgattgaa gataaaaatt tagaaagaaa tgtttttctt aaaggatata
     1501 caacaactcc acaaaaatgc ttggaagatt ttaaattagt cgtttctaca tctcaatatg
     1561 aaggtcaagg gttaagtatg atagaagcaa tgatttctaa aaggcctgtt gttgcctttg
     1621 acatcaaata cggaccaagt gattttatag aagataataa aaatggttat ttaatagaaa
     1681 accataatat taacgacatg gctgataaaa tacttcagct tgttaataat gatgtattag
     1741 cagcggagtt tggttcgaaa gcgagagaaa acattataga aaaatattca acggaatcaa
     1801 tattagaaaa atggttaaat cttttcaata gctaaatgaa aaaatcctag gtattcatga
     1861 gtacctagga tttttacgca tgataaatac aaaaaaagag aggatagtcc tctcaaacaa
     1921 tagttaataa aattgtagcc atttcttcca gctgtaacta ctaatacaag ctaagttcat
     1981 tacgatatgt atgatttcaa attcataacc acttacatat cctcttgtaa gtttaagtgt
     2041 gaagattgag cctaacataa cacaaatata aaaaacatta acataaggcg ttaaaaagcc
     2101 gataataagt gctaaacctc caacaacttc accaattgac atcaatactg cgatggtagg
     2161 tggtaaacct aaagagccta agaagtgaat tgttccatct aaatcaacaa atttcaaagt
     2221 accatgcact aagaatgatg ttgctaacat ccatctaatt aaaagcatac ctaatttcat
     2281 catgtcacct ccaacattca tatttttaaa acatagtcca aattatattt gtaaacatgt
     2341 ggaaagtaaa gattttttat aagattaatt attaaatatt ctattgtatt gaacaattgt
     2401 tgtagaaaga tgtttaagtc atcagttact ttttgtattt ataaataaag attagtacag
     2461 catagtgcaa agattatagg gaaatgaatt aactattgca tcgctattta tgaatcgtaa
     2521 acactattat gaaattgcga gagtagtatg gacaattaga atgatggttt gagataaatg
     2581 agtgttatta accagtgcgt cattttcaaa attagatgcc atttgctgtt tataataaga
     2641 agatttgata attaactatg ataagaaaaa ataataatcc cctaaataca ttataaacaa
     2701 attaaatgat aaaataataa tatatatttc tactaaagtt gtttggaggt aaatgtgtga
     2761 ataggaatat cgttaaacta gttgtgttca tgctaatttt agttgtagca gtagcgggtt
     2821 gtggtcaaaa agatactgaa gagaaaactg aaatgacgac aataaaagat gaattaggaa
     2881 ctgaaaaaat taagaaaaat cctaaacgtg ttgttgtatt agaatatagt tttgctgatt
     2941 atttagcagc attagatatg aaacctgttg gtattgcaga tgatggcagc actaaaaata
     3001 taacaaagtc agtaagagat aagattgggg catatgaatc ggttggatct agaccgcaac
     3061 cgaatatgga agtgataagt aaattaaaac cggatttgat cattgcagat gttagcagac
     3121 ataagaaaat caaatcagaa ttgagcaaaa ttgctccgac aatcatgtta gttagcggta
     3181 cgggagatta taatgcaaat attgaagcat ttaaaacagt cgctaaagca gtaggcaaag
     3241 agaaagaagg cgagaagcgt ctggaaaagc atgataaaat attagcggag attagaaaga
     3301 aaattgaaca gagtacgtta aaatctgcat ttgcattcgg tatctcaaga gcaggtatgt
     3361 ttattaataa tgaagataca tttatgggac aattcttaat taaaatgggt attcaacctg
     3421 aagtcacaaa agacaaaact acgcatgttg gtgaacgcaa gggtggtcct tatatatatt
     3481 taaataatga agaacttgcc aatatcaatc caaaagttat gattttagcc actgacggaa
     3541 aaacggacaa aaatagaacg aaattcattg atcctgcagt ttggaaatca ttaaaagctg
     3601 tgaaagataa caaagtttat gacgttgacc gaaataagtg gttgaaatca agggggatta
     3661 tcgcaagtga aagtatggca gaagatttag aaaaaattgc agaaaaagca aaataaaaat
     3721 acagcgctac tcgtaaatca tataagagtg gcgctgtatt tttaattatg tttattatct
     3781 gtcggatgtg atgatttacc tgaaagttta tttcgaataa atttaattac ataaccgaca
     3841 aggattgttt taacagttct tttaatgaat tggcgcatcg ttacatacct catttctcta
     3901 tatcttacga actatatacc cattcatata tgctttttaa acgtcattgt cacaatttaa
     3961 tttttaggga atataatata accatcttta tctgcttttt tagtaaaaat gacaaaaatt
     4021 gcatgtatta ttgagatgat ggtagggata cctgtccaga aaaataataa gtgaaaaaga
     4081 ccttgtccaa atttatcagc ataaaattta tgaataccta aacctccaag aaataatgca
     4141 acaataacat aaatggcttt attgactttc atttgtaaat cctccttaac tataattcta
     4201 cttaaattcg ttgtgaaaac caatatttct aactttagaa ttttcaaact ttctaaaatt
     4261 ataagtatat ctttttaaaa taagctagaa tttctatata ataaatgtta ataacgtaaa
     4321 agggaatgat gacatagtga tacgtcaagc acgtccagag gaccgatttg atattgcgaa
     4381 gttagtttat atggtttggg atgatatgga attagaattg gtaaagcatc tacctaaaga
     4441 catggtatta gatgcaattg aaaaaagctg tgttgatgca acatatcgaa ctttttatca
     4501 gcatatttta gtttatgaag tagaaaataa agtagcaggt tgtattatta gctatagtgg
     4561 tgaaaatgaa ttgaaatacg aaaaagcatg ggaactactt gacttgccag aaaaaataaa
     4621 acaatatggc acgccattac ctgtaaaaga agctaaagac gatgagtatt atatagaaac
     4681 aattgcgaca tttgcagcat atagaggtag aggcatcgcg acaaagttat taacgtcatt
     4741 acttgaatca aatacacatg ttaaatggag tttgaattgc gatattaata atgaagcagc
     4801 attaaagtta tataaaaaag taggctttat atctgatgga cagattgaat tatacaagca
     4861 catgtatcat catttaattg ttaaataaaa aaactcgaca gttcgatgta agtcgattgc
     4921 cgagtagtat cattttctat taaatgcctg caaataatgc actaatataa atacctaatg
     4981 catataataa accgaaaaat gtatttgttt taccagcagc agccattgct ggcatcattg
     5041 taggcggtgt atcattcttc ttgaaacgtc tgataacttt aacaggcatt gggaatgata
     5101 acaacgcaag taagtaaaat aatgagccac caggtttaat aatgatcgta agtacaataa
     5161 aggcataagc gataaagtac atgattgcca taaatgttaa agaagcattt ttacctaata
     5221 gaatgggtaa agttttgcga ccacttgctt tatctttgac acggtcgcga atattgttag
     5281 ccatattaat taaaccgata gtgattacta taggtacact taaccaaatt acataacttt
     5341 gaatattgcc agtttgaata aagaatgcaa taacgataat aaacataccc ataaatacgc
     5401 ctgagaataa ttcaccgaaa ggcgtccatg aaatagggaa agggccacct gtatataggt
     5461 aaccaacagc catacatact aatccaactg gtaataacca aaatgaagag ttagcagcta
     5521 aaaacaaacc taatattgct gctaagatgt aaaatgcaat ggctaatcgt agcacaagct
     5581 ctgggctcat accgttgcga acaatggcac caccaatgcc tacagattca tgatcatcaa
     5641 ggcctttttt ataatcatag tattcattaa acatattagt tgctgcttga ataagtaagc
     5701 atgctagtaa catggcaatg aataggctga ttttaatatg atcttcgcta ccaagaaaat
     5761 atattttaga tgctgctgta ccaactaaaa cgggtactac ggaagcagtt aatgtatgag
     5821 gacgcattaa atgccaatat ttcttaactg tagaatattg ctgatattga ttactcattg
     5881 aatacaaaac ctctttaaat catatttttt ataacaaaga tattttaata gaaagtagca
     5941 taaaggtcaa ttgacgtgaa aaattgcaag ataagtttaa gcaattcttg taacacaatt
     6001 atccaaaaat gatacaatat aataatgagt gaaaaaagac gaagaaagaa gtgaaatgga
     6061 tggctacggg cgtattagag gacgatattg tcaaagagat atatggcagc tcaaaggaat
     6121 gggtttcagt tgaagtgaaa ttatcacagt cactagaccc gagcacatta tttcatctca
     6181 ctgacaatga ggcaggagat cgcttttata tgcgtttgaa tgataatcga acgtcatatt
     6241 ttggctacaa agcaattcaa ttattcaaaa ataattctaa aaataaacaa tctattttta
     6301 aagactggga aaaattaaaa cataacatca catttataca tccgcaatct gagaaacatc
     6361 atcttcgagt tgttggaggg ttccaatttt caagtcataa atcagatgat gaatggcgag
     6421 agtttggact aaatcatttt gtattacctg aagttttaat ttcaactgat aataatggga
     6481 catttttaac ttatacagtt aaaagggaaa gttttactgt tgaggcattg aacgatttaa
     6541 tggatttgtt caacaatata tcggacatag atgtggacga gcaaattggg ggaattacta
     6601 gaaatgaaga tatttataaa gatgactggc gtcaacttgt agtagaagct atagaatcta
     6661 ttaataatga agaaaaaatt gtactagcac gtagacggtt aataaagttc gataaagata
     6721 tcagtattcc atatattcta aagcaagcat attctaaaga aaaaaacagt tatatattct
     6781 tgttagaatc acaagattct atattctttt cacaaacacc tgaacaatta ataaaggtca
     6841 ataataaaat actatcgact aaagctgtag caggtacaat taaacgttca caagatgagg
     6901 acgaagatac aaaaaatgtt gaagcatttt taaaagataa taaaaactta atcgaacatc
     6961 gatttgttgt tgacagtatt ttacatgata ttaaacctta tatcactgaa ttacattatg
     7021 ataagacgcc taaaattcta aaaaatgatc atttatatca cttgtacact gaaataaagg
     7081 cgccactgaa ggatgattcg tatattagtt taattgataa tttacatcca acacctgctt
     7141 taggtggcta tccaaaagaa tttgcaatgg attttattga gcagaaagaa tttggtacgc
     7201 gaggattata tggtgcaccg gttggctata tagatatata cgatgattgt gaatttattg
     7261 ttgcaattcg ttcgatgctt attaagaaag cacaagcaac tttatttgct gggtgtggca
     7321 ttgttaaaga ttctgatcca gatagtgaat tggcagaaac gaaccttaag ttcacaccta
     7381 tgatgaatgc attaggagtc gatatgaatg ggaaatcata aagcagcttt aacgaagcaa
     7441 gtttttacat ttgcatctga gttatatgcg tacggcgtaa gggaagtagt tatcagtccg
     7501 ggatcacgct caacgccact tgcacttgca tttgaagcac atccaaatat taaaacatgg
     7561 atacaccccg atgagcgaag tgcagcgttt tttgcagttg ggttaattaa aggcagtgaa
     7621 agacctgtcg ctatattatg tacgtcaggt acagcagcag cgaattatac gcctgcaatt
     7681 gctgaaagcc aaattagtag aattccttta atcgttttaa caagtgaccg tccgcatgaa
     7741 ttaagaagtg taggcgcacc acaagcgatt aatcaagtaa atatgtttaa taattatgta
     7801 agttatgagt tcgatatgcc tattgcggat gatagtaaag agaccattga tgcaatttac
     7861 tatcaaatgc aaattgctag tcaatattta tatggaccac ataaagggcc aattcatttt
     7921 aacttgccat ttagagatcc gttaacacct gatttgaatg caacagaatt gttaacttct
     7981 gagatgaaga ttttaccgca ctatcaaaaa agtatagatg catcggcatt aagacacatt
     8041 ttaaataaga aaaaaggttt aattattgta ggggatatgc agcaccaaga agttgatcaa
     8101 atactaacgt attcaacgat atatgatttg cctattttag ctgatccttt aagtcattta
     8161 agaaaatttg atcatccgaa tgttatctgt acatatgatt tgctgtttag aagcggctta
     8221 gacttaaatg tggatttcgt aattcgtgtt gggaaaccag tgatttctaa aaagttaaat
     8281 caatggttaa agaaaactga tgcatttcaa atattagtgc aaaacaatga taagattgat
     8341 gtctttccga tagcgccaga tatttcatat gagatttctg cgaatgattt ctttaggtca
     8401 ttaatggaag acacgacagt taatcgcgta agttggttag aaaaatggca atgcttagag
     8461 aaaaaagggc gtaaagaaat taaatgttat ttggaacaag ctacagatga gagtgcattc
     8521 gttggtgaat tgattaagaa aacatctgaa aaagatgcat tatttattag taatagtatg
     8581 cctatcagag atgtagataa cttgttattg aataaaaata tagatgtcta tgcgaatcgt
     8641 ggtgcgaatg gtattgatgg tatcgtttca actgcactgg gtatggctgt gcataaacga
     8701 ataacattat tgataggtga tttatcattt tatcatgata tgaatggact attaatgtca
     8761 aaattaaata atattcagat gaatattgta ttattgaaca acgatggtgg cggtattttt
     8821 tcatatttac cacaaaaaga aagtgcaact gactattttg aacggttgtt tggcacaccg
     8881 acgggattgg atttcgagta tacagctaag ttatatcaat tcgattttaa acgttttaac
     8941 agtgtttcag aatttaaaaa tgccacattg ttatctgaaa cttcgacgat ttatgaattg
     9001 ataacgaatc gcgaagataa ctttaaacag catcaaattt tataccagaa attgagtgaa
     9061 atgattcatg acacattata aattttatga agcaaacgtt gagaccaatc aagttttagt
     9121 atttctgcat ggatttctta gcgacagccg tacttatcat aatcacatcg aaaaatttac
     9181 tgataactat catgtcatca ctatagactt accaggccat ggcgaagatc agtcttcaat
     9241 ggatgaaacg tggaattttg attatattac gacgttgtta gaccgaattt tagataaata
     9301 taaagataaa tcaataacat tgtttggata ttcaatgggt gggcgtgttg cattatatta
     9361 tgcaattaat ggtcacatcc ctatatctaa tttgatatta gaaagtacgt caccaggtat
     9421 taaagaagaa gcaaatcaat tggaacgccg tcttgttgat gatgcacgtg ctaaagtatt
     9481 agacatagca ggtattgaat tatttgttaa tgattgggaa aagttgccat tatttcaatc
     9541 gcaactagaa ttaccagttg aaatacaaca tcaaataaga caacaacgat tgtctcaatc
     9601 gccacataaa atggccaaag cattaagaga ttatggtaca ggtcaaatgc caaacttatg
     9661 gccgcgcctg aaagaaatta aagtaccaac attaatatta gctggagaat atgatgaaaa
     9721 atttgtacag attgcgaaaa aaatggcaaa tttaattcct aatagtaaat gtaaattaat
     9781 ttctgctaca ggtcatacaa ttcatgtgga agatagtgat gaatttgata caatgatatt
     9841 aggattttta aaggaggagc aaaatgacta acagacaatg ggaaacactt agagaatatg
     9901 atgaaatcaa atatgaattt tacgaaggga ttgctaaggt aacaataaat cgccctgaag
     9961 tacgcaatgc gtttacacct aaaacagttg ctgaaatgat tgacgcattt tcacgtgcac
    10021 gtgatgatca aaacgtttca gttatcgtat taactggtga aggtgattta gcattctgtt
    10081 ctggtggtga ccagaagaaa cgtggacatg gtggttatgt aggtgaagac caaatccctc
    10141 gcttaaatgt attagattta cagcgtttaa ttcgtattat tccaaaaccg gttatcgcga
    10201 tggtaaaagg ttatgctgta ggtggcggta atgtactaaa tgttgtttgt gacttaacga
    10261 ttgctgctga taatgctatt tttggacaaa ctggtcctaa agtaggttca tttgatgcgg
    10321 gttatggttc aggatattta gcacgtatcg ttggacataa gaaagcacgt gaaatttggt
    10381 acttatgtcg tcaatacaat gcacaagaag ctttagatat gggtctagta aatacagtgg
    10441 tacctttaga gaaagttgaa gatgaaactg tgcaatggtg taaagagatt atgaaacact
    10501 caccaacagc gttacgattc cttaaagcag ctatgaatgc tgacacagat ggtttagctg
    10561 gtttacaaca aatggctggg gatgcaacat tgctttatta cacaactgat gaagcgaaag
    10621 aaggccgtga tgcgtttaaa gaaaaacgtg atcctgactt cgatcaattc cctaaattcc
    10681 cataagttat tttgaatatg atattaagtc acttgcgtcg tttattagcg acgataggtg
    10741 gcttttttat tttataagaa tttaaaaggg cattttaatt aattatcata aagataagat
    10801 agtttgagaa aaattaatag agaaagaata aatgttaaag attattatta caatgaaaaa
    10861 acgagctggt gagaaattga attctcgcca acccgcttta tgaagtgaaa tcatttaaat
    10921 tagttatact aagcgctcat aaacgattgg tcgcgaagtg ccaatacctt gtttggatga
    10981 cgtttgcatg aggatatgct gtgtatcatc ataatcaaga atataaatga tttcttcatt
    11041 atcaatagat gaaaatttaa tgcgttgatg tgccgtatct ataaaaagaa tatgatattg
    11101 attgtgactt gtatcatcgc catgacgtat acaaattgat ttttgtaatt gatgattact
    11161 ccaattacca atgaataaat tgatattggt ttgttctaga tgtgtgagtt tggttgtgtc
    11221 gtaaactaaa tttataaatt gtagttgata catagtttta cctgccttta atcaatatct
    11281 atattacttt ttagtaacct atcattgaac cataccagtt ataatcacga ttgaatgata
    11341 aatgtaatag gcttgaatct gcatcttgga ttgataattc tgtatcccaa ggattccagt
    11401 aaataagttt ttcttggtca ttaattttag cattaccaac aactgctagc gcatgtccta
    11461 aatgtgggtc attagggttt tgagatacac tttgtgcaag gatcataatt cctacattat
    11521 cttttgtaag ttgatcaact tgttcatatg atggtacgcc ttcttgataa tgaatatctc
    11581 tgccttgtga tttaccgtat tcaatcattt gattagggaa tgttgcacaa ttaggaaggt
    11641 cttgctcact tacttcaggg tataatgtac gcataatatc atgtgcatta taagtgtctg
    11701 tatttttagt tgcatttaat aatgctgcca tactgaatcc tgcacaccat gagttatcga
    11761 attgttgttc tctaattttg aagtttttta atgtattttc atattgaact tgatcttctt
    11821 gtatcgcttc gttttcttca actttagtag gtgttactgt attttttaac tcttgtttca
    11881 attttgctga aactgtttta gcactttctt tttcttttac attaccaggt agaggcgtag
    11941 cttttactaa tctaactttg ccatcttctt caaaataaaa ccctttttca tcagtaagaa
    12001 cagtgatatt tgaattttta tctttaattt ggtctaaatc tttagcgatg aagtttgaaa
    12061 ttttaacact gtaattcatg tcttcttttg atttgtttaa atcatcttta tttttaggac
    12121 taagtgttaa agtataaaca attttaccgt cttttataac tggataatag taactattat
    12181 cttcttcacc attaaattta taaattttaa aagcttcgcc aagttcatat ttaccagttt
    12241 ttgcattact ttgtttatct aatgctttag cataaccagc aaattgttgt tgtgctagat
    12301 cttttacttt ttgaggtact ttgtcactct taacattaat ttctagctgt ttagagtgtg
    12361 aatcggcttt ggctttattg ttattagcaa atgcgcctag tgataaaata agcattgaaa
    12421 ccattatgat gcttataata ttgaatactc tagatttaca tgaactattc atataatacc
    12481 ctccatttaa atcaaaatat tttacgttgc taagactttg cttctatttt atctagacga
    12541 acttaattgt taattaagct gcatctggat tgtctgaatt attgttatcg ccattgtctg
    12601 gattatcagg gttgttaggg ttgtcagggt tatttggttc atctgggttg ttagggttat
    12661 caggattgtt agggttatca ggattatctg ggttattagg ttggtcatcg ttggcaaaat
    12721 ggatatcttc aatattttgt tttaagaagt tgcgtacatt ttcattaata aataccgcac
    12781 cattaaattc atttggtaca ccgccccaat gaattccgat cacttcattt ttttcattaa
    12841 atacaggtga acctgaatta ccaccagttg tacttaaatc atattgcata gcttcgcctt
    12901 tgaggtaagt gatttttcct ttactttccc acattgttgc tacaggttta tcaccaggat
    12961 atcctgttac agtaatattt tggttaactt gtgtttcagc attattactc attgttgctg
    13021 gtttaactac ttcaccaata tgtttgtttt gctcattagg ggagaattta actattgcta
    13081 aatcaccttc gcctgaatat ttagtgattt gttcagcagt gaaaccacca tttggataat
    13141 tgtcttggtt aattgcagaa gggaatgctt ttaaagcatg aggatcaccg tgcgtagcat
    13201 ctacgacgtg tttatttgtt aaaagagtat ctttacctac aactacaccg gaagcaataa
    13261 atgtaccagt aggtgcttca acttgaatat aagttacggg tgcataatga ccattcgttg
    13321 tatctgtgat ttggtgacga tcgttatttg gtaatataac atttgcgtgt tcacgttgtt
    13381 ctaatggttt aaggttaccg cctttttgaa tcttaggtgt ttgctgtttg cttgactgcg
    13441 tttgttgtgg atgattgtcc atagcctttg aagataacgc gtttgctgct ggagaactca
    13501 caagtgtcgc tgttgtcaaa gttgcaacga ataaagaact aacttttaaa aatttacctt
    13561 tcatctaaaa acctccaaaa aatttattta caagttaaat ataacactaa aaattttt
//
LOCUS       DALAAR010000064        13603 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000064 DALAAR010000000
VERSION     DALAAR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.066"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..476)
                     /locus_tag="O2458_002059"
     CDS             complement(<1..476)
                     /locus_tag="O2458_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ3887915.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDK"
     gene            complement(537..1628)
                     /locus_tag="O2458_002060"
     CDS             complement(537..1628)
                     /locus_tag="O2458_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCZ3887916.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(1803..2105)
                     /locus_tag="O2458_002061"
     CDS             complement(1803..2105)
                     /locus_tag="O2458_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887917.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(2119..2439)
                     /locus_tag="O2458_002062"
     CDS             complement(2119..2439)
                     /locus_tag="O2458_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCZ3887918.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(2590..2874)
                     /locus_tag="O2458_002063"
     CDS             complement(2590..2874)
                     /locus_tag="O2458_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887919.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(2883..3311)
                     /locus_tag="O2458_002064"
                     /pseudo
     CDS             complement(2883..3311)
                     /locus_tag="O2458_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(3506..3730)
                     /locus_tag="O2458_002065"
     CDS             complement(3506..3730)
                     /locus_tag="O2458_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCZ3887920.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(3747..3950)
                     /locus_tag="O2458_002066"
     CDS             complement(3747..3950)
                     /locus_tag="O2458_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCZ3887921.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            4109..4669
                     /locus_tag="O2458_002067"
     CDS             4109..4669
                     /locus_tag="O2458_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCZ3887922.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            4688..7090
                     /gene="mnhA2"
                     /locus_tag="O2458_002068"
     CDS             4688..7090
                     /gene="mnhA2"
                     /locus_tag="O2458_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCZ3887923.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            7077..7502
                     /gene="mnhB2"
                     /locus_tag="O2458_002069"
     CDS             7077..7502
                     /gene="mnhB2"
                     /locus_tag="O2458_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCZ3887924.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            7499..7843
                     /gene="mnhC2"
                     /locus_tag="O2458_002070"
     CDS             7499..7843
                     /gene="mnhC2"
                     /locus_tag="O2458_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCZ3887925.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            7833..9329
                     /gene="mnhD2"
                     /locus_tag="O2458_002071"
     CDS             7833..9329
                     /gene="mnhD2"
                     /locus_tag="O2458_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCZ3887926.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            9330..9812
                     /gene="mnhE2"
                     /locus_tag="O2458_002072"
     CDS             9330..9812
                     /gene="mnhE2"
                     /locus_tag="O2458_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCZ3887927.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            9809..10111
                     /gene="mnhF2"
                     /locus_tag="O2458_002073"
     CDS             9809..10111
                     /gene="mnhF2"
                     /locus_tag="O2458_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCZ3887928.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            10086..10523
                     /gene="mnhG2"
                     /locus_tag="O2458_002074"
     CDS             10086..10523
                     /gene="mnhG2"
                     /locus_tag="O2458_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCZ3887929.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            10863..12905
                     /locus_tag="O2458_002075"
     CDS             10863..12905
                     /locus_tag="O2458_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ3887930.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         4438 a         2021 c         2236 g         4908 t
ORIGIN      
        1 tgtttatctt tacgtttaat attataaaat tctttactat tcaattttcg ttctgtatct
       61 gaaatttttt catcaggttt tttataatct gatggaaaat atttcaacaa ttctttttct
      121 ctagattcca attcttttga atctttcgag atattcatat acgtatcaat aaacttatca
      181 gcatataact ttaatttagg actatactga actgtattcg cattatcttc atactttttt
      241 tgaatcatat tcgttttatt aactgcttct ttactgtcat tatttgctct tgtagcttta
      301 ataaaagaag ctaataaaag aattaacaaa atagctaata atgagtaaaa acaaattaca
      361 atcatcttac gacgattttt aaattttgga attactcggc gttgttcttt aggaatatca
      421 aaatgctttc cgtttttaga aaatcgtgtt aaaatgtact tgcttattat tttcataaac
      481 gattttctaa aatcctttac gaaattttta ataaaaatca catcccagtt atttttttaa
      541 cacttatctt ttgataagtg ctttttttgt tttaaagcct ccaaataatg attaattcta
      601 ttcgtatgct catcattaaa ttgtgaatgc gctaaaattt tatctaaaaa atcagtatct
      661 gtaagcaacc cctcagattg cgccatactt tcggcagtta acactgtatc taaagtagta
      721 gcaacttgtt tacataacca atcaagatta tcctcaatgg actttaatgt tggtctagta
      781 gttaaattca atttttcagt atctttaata aataacgccc aaggtctata taatggccac
      841 cgcttacgat ttttatcatt tccaccttta ggaggagaga taaaacgcaa attattatgc
      901 aatatagact ttacaatttc gcttatatta tctgttctac tcaacacttt cgcacaatta
      961 actgcatatg cctttcgcat ttgtatttca taacgattcc atagcccaat gtcttcgaca
     1021 tcacaatgac gtttaaacgc ttgttcataa ttcttttcat aaaaacgaca atataaattg
     1081 ctttgtttag aaccaatata aaaagtagcg cctttacttc ttccgtctga caaattaaaa
     1141 ccgtttattg cgcttccagc tctaaaacgt gaaacacact cagctttttc agccttttta
     1201 attaataaag gcacttttaa atatgtttta taatcatcaa ttgcaatatc aattcttgta
     1261 aaatttgcat taactgataa acatttgtca aaaaaatctt gccaagtttt attcaactgc
     1321 tgtaaatgat gttcaaactc tctacaacct gtaccagtta actgtaagtg aatgcccatt
     1381 gattcatcat cacttgaata atacaattta atgtttgaaa attcataacc gcgattataa
     1441 aagtaaatgc cactatttcg ttttttgaac aacgttatag gcaaaccgat aacgtcctca
     1501 attactgctg aaataggggc tatatggaaa gtgacctgaa cccagtcaac aactgcctcc
     1561 acagcacttt tatttgtacg ctctaccccc ctattagtaa ggggggtaca ctggtttttt
     1621 aaagtcatat ttatttatgc ctcctatcaa atcgcaagcg atttgataca tgaccgttct
     1681 cgctttttta acgcgtattt gtcgcaggct ccgcccaaat gcttaacgca ctccgtgctt
     1741 gcatttctgc gcctgcatac gctaaaagct acacggtcat gttaaatcac taatactata
     1801 tgttaaacat caatatcgcc aatataaata caatcttcca aattcacttg tagcattctg
     1861 ccatgtcctg cttcactcaa gctcatacac aatagacaaa ggtcagcgta aattttaggc
     1921 cgacctttta ccgtattttc tttaacgtaa ctatcattaa atttagactg tatttcttta
     1981 gctaaagtca ttaatgaaac tttatcgccc caatttatac gactagcaac atagtttaaa
     2041 ataggttcac tcaaatttga actatttgtg tagcgatata atctatctgc tgtataataa
     2101 tccatttaca tcacctaatt atttttgttt aagtgcacta ttagaaccaa ctactttaat
     2161 atcttctgca ttccagctat atgcgatagt tccaaaatcg cctgacattt gaacatacat
     2221 tttaacgtct ggttcaacta attcgcattc ttgtaaatag tctaattcca ctttattagg
     2281 cgttttaaca gtgatttgtt cgccttgttc ttgactcgct aatttatatg cataaacagt
     2341 tttatcagta cgctctccat tttcatattt atatttttca tctacaccta aaaagtttaa
     2401 ttgtcctaaa gtttttttaa tatccaatac tggtttcata atatatccat cctttacaaa
     2461 atttttaata aaattaaacg acttctaaag ggtcgcacga ataggaataa ctcccgcaaa
     2521 tccgtaccac tgcatttttg ataatggagt gtctatgctt ctcctgacct atcatatttt
     2581 taatcaaacc tatcggttca tcacctcaaa aaattctaag tcttcaattg aaatatgata
     2641 catcttacaa ataaatgtat tagacacacc atattgtttt aaaaatttat aatccttata
     2701 agtaaatcga taatcattct tttttgccat ttcacaaact tctaatatac gaaaatatgc
     2761 aaaattatac attttacaaa tttttctttt agaaacacca tgaaatctta aaattacaaa
     2821 tttaagataa tttaattgat ttgaaccatc aaaaaaaggt gtaaactcag tcataattat
     2881 caattccttt cataagaatt aactgttaac taaagtttac accaccaatt aaaatcatga
     2941 attgtagcgc caaataatcc aaatgtatca ataatgacac ccataataat ttgacccgca
     3001 actgttgcaa ttacagttaa tgttgcacct aattttggca ataacaataa attgccagtt
     3061 aaaaagctaa ccccaagcaa accaccgact acccatgtgt agttaaatga ttgattattg
     3121 taaaagtgaa tagtaaatac ttctggattg ataatgatat ttaaaataat taaacaaatt
     3181 gttccaactg aaaatgaaat gaatgaagta tagaaaggcg atttagtata cagtgatagt
     3241 cttgaattga cagatgtttg gataggaata agcattccaa ctaggacacc aatgatatag
     3301 aaaagaagca tatgaataaa gtctcacttt ccaaagttaa taataaaatc atatatttac
     3361 attatcatta tagagaaaat atacaactca tttacaataa tgttgaaaaa aacaggacat
     3421 tattacaaca aaatcaccct agacaatcat gatgaaagtc tagggtgtta gtatttatac
     3481 taaattaatt gcgctttctt tatatctatt tcttatcttc aaataaaaca gttgttgttt
     3541 tatcaataat ttgagcagaa tgtactttgg tgatagtacc actatttgac tttataatat
     3601 ttaaatctac taatgcattc atactatcac gtgcaacttg ctcggtcact acgttgttta
     3661 attgtggtaa ttgcagtttt acaggcttgt ctagtgaaga tttaaaactt aattcaagtg
     3721 ttttagtcat gaatatgtcc tcctgattaa attgataaag atttgatgag ttcgatattg
     3781 ctatatgttt ctccagtaag tcgctcaata agtttgctga atgttttaat ttggtcgttt
     3841 gatgcatcag ggttaatgtt agcgaatcga cgcttaaatt ctgtttgttt gccgttagcg
     3901 tctactttag taaatgataa tacaatagtg atgtggttta ttttactcat attttaaaac
     3961 ctcctttcac actatatatc gaaacaaaat aataaaatgg ctaattttat tttctatgtt
     4021 taaaatctat aaaaaaggca atagatatat gtaactaaaa tataatatag ctatgattaa
     4081 gacacttaaa gcaaggggag gttgagtaat gaataaagta gaagcgatta aatttaatga
     4141 tgatattgtt aaaatgtatg aagcgctcaa gataaaatct gaacgtgact atttattctt
     4201 taagttagct atacatagtg gattgaaagt atcagaatta ttaacaatta cagtctctca
     4261 agttaagaga ctaattgaaa agtgtacgtt atcagaaatg tgtaaagcac attttcattc
     4321 gttgattaaa attaggttac cagaaacatt atcgaaagaa ctacttcaat atatagagga
     4381 caggagtctt tcgaatgaag acgttctttt tcaatcacta cgaacaaatc aagtattatc
     4441 tagacagcaa gcatatcgaa taattcacca agcatcaatt gaagctggta tagataatgt
     4501 aggactaacg acattgcgta agacatttgc atatcatgct tatcaaaaag gtatacctat
     4561 accagtcatt caaaagtatt tagggcatca atctgctatt gaaacactaa attttatcgg
     4621 tttagaaaat gagtgtgaac atagtattta tatttcatta caattataga aacaaaggag
     4681 gctaataatg agtttggttt atttattaat tgctatactt gtgattatgg cgatgatact
     4741 tctaatgtct aaacgtagag cattggctaa atatgccggg tacatagcgt tggttgcacc
     4801 tgtaatttca tctatctatt ttttgattca aataccatca gtagctaaac tgcaatatct
     4861 ttctacctct attccatgga ttaagacatt agatattaat ttagatttac gtttagatgg
     4921 tttaagttta atgttttctc ttattatttc acttattgga attgcagtat tcttctatgc
     4981 aactcaatat ttatcctctc gaaaagacaa tttaccaagg ttttattttt atttaacgtt
     5041 atttatgttc agtatgattg gtattgtatt atcagacaat acgatattga tgtacatttt
     5101 ttgggaatta acgagtgtat catcattttt attgatttca tattggtata acaatggtga
     5161 cagtcaattt ggtgcgattc aatcatttat gattacagta tttggtggat tggcgttatt
     5221 agttggtttt attatgctgt atatcatgac aggaacgaat aacatcacag agatattagg
     5281 acaagcagat catattaaga atcatggatt gtttatccct atgattttta tgtttttatt
     5341 aggtgcattt acaaaatcag cacaatttcc atttcatatt tggctaccta gagcaatggc
     5401 tgcacctaca cctgtaagtg cttatttaca ttcagccacg atggtaaaag ctggtatctt
     5461 tttattactt cgatttacac cattattagg tcttagcaat atgtacgtat atatcgttac
     5521 gtttgttggt ttaataacaa tgttatttgg ttcaattaca gctttaaaac aatgggattt
     5581 aaaaggtatc ctagcgtact ctacaatcag tcaacttggg atgattatgg ctatggtggg
     5641 tataggtggc ggatatgctc aacaccaaca agacgcaata gcatctattt atgtatttgt
     5701 attatttggt gcgctatttc atctaatgaa tcatgccatc tttaaatgtg cgcttttcat
     5761 gggagtaggt attttagatc atgaagcagg ttcaagggat atacgaattt taagtggaat
     5821 gcgtcaacta tttcctaaaa tgaatctagt catgacgata gcggctctat ctatggctgg
     5881 agtaccattt ttaaatggat ttttaagtaa agaaatgttt ttagatgcat taacacaaac
     5941 tggacaatta tcccaattta gtttgatttc aatgatagct atcgtgtttg ttggtgttat
     6001 tgcgagtgtt tttacattca catatgcact atacatggta aaagaagtat tttggacaaa
     6061 atatgattct aaggttttta ctaaaaaaaa tatccacgaa ccatggttgt ttagtttacc
     6121 atctcttata ttaatggtgc tagtacctgt aatctttttt gtaccaaata tatttgggaa
     6181 ggggattatc gttctagcat taagagctgt atcaggtggt aatcatcaaa ttgatcaatt
     6241 ggcaccacat gtttcgcaat ggcatggatt taacataccg cttcttttaa ccatcatcat
     6301 tattttattg ggtagtgtac tagcaatcaa agtagattgg aaaaaagtgt tcacaggtaa
     6361 aattagacag atttcagttt caaaaagcta tgagatggta tatcgacatt ttgaaaagtt
     6421 tgctacgaag cgatttaaac gtgttatgca agatcgttta aaccaataca ttattatgac
     6481 cttaggcata tttatgatta tcattggata tggttatatt cgaattggac ttcctaaagt
     6541 acatcagtta catgtttctg aatttggggc attagaaatt atattagcaa tcgtaactgt
     6601 cacaattggt atttctttaa tttttatacg tcaacgactg acaatggtca ttttaaatgg
     6661 agtcatcgga tttgttgtga ccttattctt tatagcaatg aaagcccctg atctagcatt
     6721 gactcagcta gtagttgaaa caataacgac gatactattt attgtcagtt tttcaagatt
     6781 accaaacgtg ccaagatcta acgctaacaa aaaaagagaa ataattaaaa tttctgtatc
     6841 actcttgatg gcacttattg ttgtatcatt aatttttatt acacaacaaa cagatggttt
     6901 atcatcaata tcagactttt atttaaaagc tgacaaacta acaggtggta aaaatattgt
     6961 aaatgcgata cttggtgact ttagagcatt agatacatta tttgaaggat tagtgttaat
     7021 tattactggg ctaggtattt acacattatt aaattatcaa gatcggaggg gacaagatga
     7081 aagagaatga tgtcgtgtta agaacggtca cgaaacttgt tgtatttatt ttattgactt
     7141 tcggattcta tgtcttcttc gcaggtcata ataatcctgg tggtgggttt attggtggtt
     7201 taatatttag ttcagcgttt attttaatgt ttctggcttt taatgttgaa gaggttttag
     7261 aaagtttacc gattgatttt agaattttaa tgattattgg agcattggta tcatctatta
     7321 ctgcgataat acctatgttt tttggaaaac catttttgtc tcaatatgaa acaacttgga
     7381 tacttccaat tttaggacaa attcatgtaa gtacaataac actttttgaa ttaggtattt
     7441 tattctcagt tgttggtgtt attgtcacag tgatgttgtc gcttagcgga ggtcgatcat
     7501 gaatttaata ttattactag ttataggatt tttagtgttt ataggaacat atatgatttt
     7561 atcaatcaat ttaattcgta ttgtaatcgg aatttcaata tatactcatg ctggtaatct
     7621 cattattatg agtatgggaa cgtatggttc tagtagatca gaaccactaa taactggtgg
     7681 aaaccaattg tttgttgatc ccttgttaca agctattgta ctaactgcaa tagttatagg
     7741 gtttgggatg actgcgtttt tacttgtact tgtttataga acttataaag taacaaaaga
     7801 agatgaaatt gaaggcctaa ggggggaaga tgatgctaag taacttattg attttaccaa
     7861 tgttattacc attcctttgt gccttaatcc ttgtattttt aaaaaataat gatcgtattt
     7921 ctaaatattt atacttaggt acaatgacta tcaccacaat tatttcatta atgctattaa
     7981 tttatgttca gcgtcaccgt ccaattacgc tagactttgg aggatggtca gcgccctttg
     8041 gtatacagtt tttaggagat tctttaagtt taattatggt tacaaccgct tcgtttgtga
     8101 ttactttaat tatggcatac ggatttgggc gtggcgaaca taaagcaaat cgttatcact
     8161 tgccatcgtt catattattt ttaagtgttg gcgtgatagg ctcttttcta acatcagatt
     8221 tatttaattt atacgtcatg tttgaaatta tgttactagc gtcatttgta ctcattacac
     8281 ttggacaatc tgtagaacaa ttacgtgctg caattattta tgttgtcttg aatattattg
     8341 gttcatggct attcttatta ggtataggtt tactttataa aacagtaggt acattaaact
     8401 tttcacatat tgcaatgcgt ttgaatgaca tgggagataa tcgcactgtt acaatgattt
     8461 cattaatctt cttagtcgca tttagtgcga aagcagcgct ggtccttttt atgtggctac
     8521 ccaaagccta cgctgtgtta aatactgagc ttgcagcatt atttgcagcg ttaatgacca
     8581 aagtaggggc ctatgcatta attcgattct tcactttact atttgatcaa cataatgatc
     8641 tcatacatcc attgctagca actatggctg ctataactat ggtcatcggc gctataggtg
     8701 tcattgctta taaagatatt aaaaagattg cagcttacca agtcataatc tcaataggat
     8761 ttatcatttt aggtttagga acaaacacgt ttgcaggtat taatggtgca atattttatt
     8821 tggtaaatga cattgttgta aaaacattgc tattttttat tattggtagt ttagtttaca
     8881 ttacaggcta tcgacaatat caatatttga atggcttagc taaaaaagaa cctttatttg
     8941 gagttgcgtt tattataatg atttttgcta ttggcggcgt gcctccattt agtggctttc
     9001 cggggaaagt acttattttc caaggtgcat tgcaaaatgg caattatatt ggactagcgt
     9061 taatgattat tactagtcta attgcaatgt acagtttatt taggatactt ttttatatgt
     9121 attttggaga taaagatggg gaggaagtta attttaagaa aatcccgcta tatcgaaaaa
     9181 gaattttaag tattttagta gttgtggtta tcgcaatcgg aattgctgca cctgttgtgt
     9241 taaatgttac aagtgatgca actgagttga acacgagtga tcaattatat caaaaacttg
     9301 taaatccgca tttgaaagga gaggactaaa tgaatcaaat agttttaaat attatcattg
     9361 cattcttatg ggtattattt caagatgaag atcattttaa attctcgact ttcttttctg
     9421 gatatctaat tggtttaatt gtcatttata tattacacag gtttttcagc gatgattttt
     9481 atgttagaaa aatatgggta gctattaaat ttttaggtgt ttatttatat caattaataa
     9541 catctagcat tagcacgatt aattatattc tttttaaaac aaaagatatg aaccctggat
     9601 tactttcata tgaaacaaga ctaacaagtg attggtcaat aacattttta acaattttaa
     9661 ttattataac tccagggtct acagtaatac gaatttctca agactctaaa aagtttttta
     9721 ttcatagtat cgacgtgtca gaaaaagaaa aagatagttt gttaagaagt attaagcatt
     9781 atgaagactt aatattggag gtgtcgcgat gatacaaaca ataacacata ttatgattat
     9841 tagttcactc attatttttg gaattgcatt aatcatctgt ttatttagat taatcaaggg
     9901 acctacaaca gcagatcgtg tcgttacatt tgatacaaca agtgctgtcg taatgtcaat
     9961 tgtgggtgtg ttaagtgtac ttatgggcac cgtttctttc ttagattcaa tcatgctcat
    10021 tgccattata tcttttgtaa gttctgtttc aatatcacgc tttattggtg gggggcatgt
    10081 gtttaatgga aataacaaaa gaaatcttta gtcttattgc tgctgtgatg ttgttgttag
    10141 gtagttttat tgctcttatt agtgcaatag gtatcgtgaa attccaagat gttttcttaa
    10201 gaagtcacgc tgcgacaaaa agttcaactt tatccgtgtt attaacttta atcggtgtat
    10261 taatttattt tattgtgaat acaggatttt tcagtgtgcg tttattactg tcacttgttt
    10321 ttattaattt aacttcacca gtcggcatgc acttagtcgc tcgcgctgct tatcgcaacg
    10381 gcgcttatat gtatcgaaaa aatgatgctc acacacatgc atcaatatta ttaagttcaa
    10441 atgaacaaaa ctctacagaa gcattacaat tacgtgctga aaaacgagaa gagcatcgta
    10501 agaaatggta tcaaaacgat tgaagtgttg gctaaattgt taagcataac aatgcttttg
    10561 aaaatctgtt ttcaaaatta atcattaaaa gagtactttg aaattggtta gtcgatttta
    10621 aggtgctcat tttttgtatt ataagttaat gttgttatgt gaaagcaagt tgtttattta
    10681 tacaaacaat tagtgataaa ataaaattaa ataattgcaa atttaagtac tgcaatcgta
    10741 gacatatatc tatattaaag tgcttttgtg gtcgctaact caatttatcc tctaaagtat
    10801 aattacaacg agatgtgata cttaattttc aattaaacaa tactttttag agaggtgaaa
    10861 gtttggaaat atttgaaaca attcttatat ttatagctgt tgtgatacta agttcgtttg
    10921 tccatacttt catacctaaa gtacccctag catttataca aattttcttg ggcatgttac
    10981 tatttattac cccaatccct gttcaattta attttgattc tgaattgttt atggtaacaa
    11041 tgattgcgcc tttgttattt gtagaaggtg ttaatgtttc tagagtccat ttaaggaaat
    11101 atattaagcc agtgatgatg atggcattag gattagtcat tactactgtg ataggtgtag
    11161 gtttatttat tcattggatt tggccagatt tacctattgg agcagcattt gcaattgctg
    11221 ccattctttg tcctactgat gcagtagcag tgcaagcaat cactaaagga aaggtcttgc
    11281 caaaaggagc aatgacaatt cttgaaggtg agtcattatt gaatgatgct gctggtatta
    11341 tttcatttaa aatagctgtt ggagtattag ttacaggtgc tttttcactt gttgatgctg
    11401 ttcagttgtt tttaattgca tcaattggtg gcgcagtggt tggtttactt ataggtatgg
    11461 cattagtaag gttccgatta acattgatgc gtcgaggata tgaaaacatt aatatgttta
    11521 caattattca attgttaaca ccatttgtta cgtatttaat tgctgaattg tttcacgcat
    11581 caggaatcat tgcagcagta gttgcaggac ttgtacatgg tttcgaacgt gacagaatta
    11641 tgcaagtacg tacacaactg caaatgagtt acaatcatac atggaatata ctaggttatg
    11701 ttttaaatgg ctttgttttt tcaatattag gatttttagt acctgaagtt attattaaaa
    11761 ttatcaaaac agaaccgcac aatttaatct ttttaatagg catcactatt gttgttgctt
    11821 tagctgtcta tctatttaga tttgtttggg tttatgtctt atatccttat ttttatttag
    11881 ccatcagtcc attccaaaaa atgatgacta aaaatgatga tgataatcca acgactgaga
    11941 aaccaccaaa gcgaagttta tacgctttaa ttatgacgtt atgtggtgtg catggaacaa
    12001 tttctttagc aatcgcatta acgttaccgt attttttagc agggcatcat gcttttacgt
    12061 atagaaacga cttattattt attgcatctg gtatggttat tattagtttg gtagttgcgc
    12121 aagtattatt gccattatta acgaaacctg cacctaaaac agtaattggc aatatgtcgt
    12181 ttaaagttgc tagaatttat atattagaac aagttattga ttatctaaat caaaaatcta
    12241 ctttcgaaac aagttttaaa tatggtaacg tgattaaaga atatcatgat aaattagcat
    12301 ttttaaaaac tgtagagaaa gatgatgaaa actctaaaga attagaacgt ctacaaaaaa
    12361 ttgcttttaa tgtagaaaca aaaacattag agtctttagt agatgaagga caaataacga
    12421 atagtgtact tgaaaactat atgcgttatg ctgaaagaac acaggtatat agacaagcat
    12481 cattaataag aagaatgatt gtattattac gaggtgcttt attaaaacga agagtacaaa
    12541 cgagagtgaa ctccgcatct tcacttagtg ttacggataa cttaatggaa ttaaataaaa
    12601 ttaataaatt agtccattat aatgtggtta gtcgtttgtc taaggaaaca acaaaagata
    12661 atacacttga agttggaatg gtttgtgacg gttatttaat gcgaattgaa aacttaacac
    12721 catcaaattt cttcaactca gcaagtgaag atacgattac taaaattaaa ttaaatgcat
    12781 tgagagaaca acgtcgcatt ttacgtgagt tgattgatac agatgaagta tcagaaggta
    12841 cagcgttaaa actaagagaa gccatcaatt atgatgaaat ggttattgta gatagtatga
    12901 cgtagttcct aattatgcta aaagggattg atgaaaaact gaagggcttt tcatcaatcc
    12961 cttttatttt aggggaattg aatagatagt tttaaactat acgaattatt aatatttgag
    13021 atttaattga aataagtttt aaaaattgga ggagatagat taagcgaagt catttaaagg
    13081 tgaagttaag tgtattcaca aaaaatagcc acactcatat gacatcggat gagtgtggct
    13141 taaggatcta tggggggagg aaaccataga tgtttacttt gataggccag attaaatatc
    13201 aaagtatgcg attatttata gcttgatgca aaagtggtat gcctattaaa agttactgca
    13261 catagctttt aatattccgt tcaaaggaaa ggggcataca attgaacaat ctgtaatagt
    13321 acttttaacc agctatgcta aaagtctagt agggagaaca gttgtccaat cacataagaa
    13381 cctctaactt cgttagtacg attaagaaaa gctttttagt tagtatgtaa tacaatttat
    13441 tgacgcgcgt gaatctcttt tataagagtg tgtagggaat ggcgttgtat aaattgtatt
    13501 agaagaactt ctaacgcatc tctgtggtta aaagagatga agggaacgac agtttaatta
    13561 aaactgcata agaacttcta gcttttctct ctcgttcaaa gag
//
LOCUS       DALAAR010000065        13252 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000065 DALAAR010000000
VERSION     DALAAR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.067"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            235..1305
                     /locus_tag="O2458_002076"
     CDS             235..1305
                     /locus_tag="O2458_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCZ3887931.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            1501..2262
                     /locus_tag="O2458_002077"
     CDS             1501..2262
                     /locus_tag="O2458_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ3887932.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            2280..2774
                     /locus_tag="O2458_002078"
     CDS             2280..2774
                     /locus_tag="O2458_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ3887933.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            2788..4014
                     /gene="pepT"
                     /locus_tag="O2458_002079"
     CDS             2788..4014
                     /gene="pepT"
                     /locus_tag="O2458_002079"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCZ3887934.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(4203..5327)
                     /locus_tag="O2458_002080"
     CDS             complement(4203..5327)
                     /locus_tag="O2458_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ3887935.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(5752..6072)
                     /gene="ytxJ"
                     /locus_tag="O2458_002081"
     CDS             complement(5752..6072)
                     /gene="ytxJ"
                     /locus_tag="O2458_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCZ3887936.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(6226..7104)
                     /locus_tag="O2458_002082"
     CDS             complement(6226..7104)
                     /locus_tag="O2458_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCZ3887937.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            7224..7742
                     /locus_tag="O2458_002083"
     CDS             7224..7742
                     /locus_tag="O2458_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCZ3887938.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            7869..8792
                     /locus_tag="O2458_002084"
     CDS             7869..8792
                     /locus_tag="O2458_002084"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCZ3887939.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            8810..9124
                     /locus_tag="O2458_002085"
     CDS             8810..9124
                     /locus_tag="O2458_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887940.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9441..10469)
                     /locus_tag="O2458_002086"
     CDS             complement(9441..10469)
                     /locus_tag="O2458_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3887941.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(10588..11349)
                     /locus_tag="O2458_002087"
     CDS             complement(10588..11349)
                     /locus_tag="O2458_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3887942.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(11346..12302)
                     /locus_tag="O2458_002088"
     CDS             complement(11346..12302)
                     /locus_tag="O2458_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ3887943.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(12289..13236)
                     /locus_tag="O2458_002089"
     CDS             complement(12289..13236)
                     /locus_tag="O2458_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3887944.1"
                     /translation="MLLLLLILLTIISLFIGVSELSIKDLLHLTESQRNILFSSRIPR
                     TMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFPTGHILLKL
                     VFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLRTNAVQSIG
                     NWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGLSYEKLINI
                     ALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGAIFVLFSDI
                     VGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
BASE COUNT         4418 a         2288 c         1891 g         4655 t
ORIGIN      
        1 cgtatatctt atagctttat tataaattaa ttcgttcaat caatgatatc atatttacaa
       61 ctatacgatt catttcttat atatcgccta ttattcattt tttcatcata tcatttaagg
      121 caacatttta acatcaaagt ctatatcata cattaatttt atatgtaaac taatgacaaa
      181 ttgtaagttt tcacttacaa tagctagtgg tcaattaaag gagtcgattc agacatgttc
      241 gaagcattta tatacaatat atctgttata gtcgctggaa tctatttatt ccatcgctta
      301 cagtattcag aaaacaaacg tatggttttt tctaaggctt atgtcactgt tttaatgaca
      361 atcgtatcat tattattatc tgtctaccct atcccttatc gtgaagatta tttaattcac
      421 ttaacattcg ttcctttatt gtttttaggt agatttacaa atatggtcta tacattatct
      481 gccacagtaa ttgtagcaat tgttgaaatt gtagtattta acaactcgat tatgtacggt
      541 gttacgttga ttgttattgc tgcagtaaca agtgctattg gaccattttt aaaacaaaat
      601 gatgtgctat cattattaat acttaatgtt gttacaataa tcattttatt tggtgtcgcg
      661 ttagtgagtc ctatttacac actatcggaa gttatcattt taataccaat ttcattaatt
      721 atcactttag cttcagcgat aacatttgtc gatatttggc atttcttctc actcgtaaat
      781 cgttatgaaa acgaagataa atatgattac ttaacaggct taggtaatgt aaaagaattt
      841 gatagacatt taaatgaaat ttcacgaaaa gctgaaaaag aacatcaaag tatcgcatta
      901 ttattaatcg atatcgatgg atttaaagat gtcaatgata cctactcaca caaatcaggt
      961 gatgctgtat taaaacaaat gtctcaatta cttaaaaact atgtgccgaa tcaatttaaa
     1021 atttttagaa atggtggcga agagttctca gttgtcattc acaattattc acttgatcaa
     1081 agtgttaaat tagctgaaaa tattcgttcg ggtgttgaaa aatcatcatt ccatttacca
     1141 aacaaagaag ttattaaatt gtcagtgtct atcggtgttg gttatttaac agatgacgat
     1201 ccaaaatcac agcgtaaagt gtttaaagat gcagacgata tggtgcatgt agccaaaaat
     1261 caagggcgaa acaaagtaat gtttaaccct attatcaatt tataacaacg acaaaactgt
     1321 taatcctttg ctattattaa atcatagtat aatagcaaag attaacagtt tttcttattt
     1381 aaatttgaac catacaagtt catcatacta acacaaagtc tcactattga aagttgtaac
     1441 aacaagttgt aaacttcaat tgttcaaata aataacacat atcattggag gtgaattgat
     1501 atgccagatt caatcacaat tatagatgaa aacaaagtga ttgatgttgt attaattgca
     1561 ggtagaattt tacttgaatc aggtgctgaa acatatcgag ttgaagatac aatgaaccgt
     1621 atcgcacata gttatggtct tcataataca tatagttttg tcagttcaac tgcaattatt
     1681 ttttcattaa acgatcgaac aagtacaaga ttaattcgtg tacaagagcg tacaacagat
     1741 ttagagaaaa tcgctttaac gaatagtctt tcacgtaaaa tatcaaataa agaactcaca
     1801 attgatgaag ctaaatctga atttattcat ttacagcatg catcattaca atattctttt
     1861 ttaacgaatt tctttgcagc tgccattgca tgtggcttct tcctatttat gtttggtggt
     1921 gtcgcatcag attgttggat tgcagtcatt gctggcggat ccgcattttt aacattcagc
     1981 tttgtgcaac gctatataca aattaaattt ttctcagagt ttgttgcagc cgctgtggtc
     2041 atatccattg cagccacatt tactaaatta ggcattgcaa ccaatcaaga tatcattact
     2101 atagccagtg tcatgccact tgtccctggt atattaatta ccaatgccat tcgagattta
     2161 ttagcgggtg aattacttgc aggtatgtct cgcggtgttg aagctgcatt aacagcattc
     2221 gcaatcggtg ctggtgtcgc aatcgtttta ttaatcattt aaggaaggac tgttaaagca
     2281 tgctatttta tttatttcat tttacaatca gctttatatc aacagtactt ttctctatca
     2341 ttttcaatgc acccaaacgc ctcttagtag catgtggatt tgtgggtgcc attgcatgga
     2401 cgatttacca attaacggta gatttagagt ttggaaaagt tggcgcttca tttttgggaa
     2461 gcttaatttt aggcttaatg agtcatacta tgagtcgcag atataaacga ccggtaatta
     2521 tattcatagt gccaggcatt ataccattag tacctggtgg tgcagcttat caagcgactc
     2581 gttttttagt atcaaatgat tatacaagtg ctgtaaatac atttttagaa gttacactga
     2641 tttcaggtgc gattgctttc ggtatattag tttctgaaat tctatattac ctatacacac
     2701 gtatcaaaca actgtatggt aaaatcaaag gtaagacata taaaaaatct tacaacatga
     2761 ataatagagt ttaaggggtc gaatataatg aagaaccaat tgatagatag attaacaaga
     2821 tatacgacaa ttgatacgca atctgatcca aaatctacaa caacaccttc aactgaaaag
     2881 caatgggatt tgttacattt attagaaaaa gaattacaac aattaggatt gccaactgat
     2941 ttagatgaaa atggctactt atttgctaca ttagaaagta acatcgatgt cgacgtccca
     3001 acagttggat ttttggcgca tgttgataca tcacctgatt tcaatgcttc aaatgttaaa
     3061 ccgcaaatta ttgaaaacta tgatggtaag ccatacaaac taggcaatac taaacgtgtc
     3121 ttagatccta aagtatttcc agaacttaac agtttagttg gtcatacatt aatggtaact
     3181 gatggtacat cgttactagg tgcagacgat aaagcgggta ttgtggaaat tatggaagct
     3241 atttgttatt tacaagaaca tccagaaatt aaacatggta ccattcgcat tggatttaca
     3301 ccagacgaag aaatcggtcg tggtccacat aaatttgatg ttgaccgctt caatgctgat
     3361 tttgcttata ctatggatgg tagtcaatat ggagaattac aatatgaaag ctttaacgct
     3421 gctgaagcgg ttattacatg ccacggtgta aatgttcatc ctggttcagc taaaaatgca
     3481 atggtaaacg caatacgttt aggtgaacaa ttcgatagtt tgctacctga tagtgaagtt
     3541 ccggagcgaa cagaaggata cgaaggcttt tatcacttaa tgaactttga aggaactgtt
     3601 gaaaaagcaa ctttgcaata cattattcgt gatcatgata aaaaacaatt cgaattgcgt
     3661 aagaaacgta ttttagaaat acgtgacgat atcaatgccc attttgaaaa ttatccagtt
     3721 aaagttgata tatcggatca atatttcaat atggcagaaa aaatattacc attgcctcat
     3781 attattgata tacctaaacg tgtctttgcc aaattagata ttccagcaaa cactgaacct
     3841 attcgcggtg gtacagatgg ttcacaattg tcatttatgg ggttaccaac tccaaacatc
     3901 tttacaggtt gcggtaactt ccacggccca tatgaatatg catccattga tgttatggaa
     3961 aaagcagtac aagtaatcat tggaattgta gaagatatcg ctgaaaatca ctaaaaacca
     4021 acgattatat actagttcaa ctcaggctta attaatatat aaaaatcacg ctaacattca
     4081 gtgcaatgat agcgtgattt ttcatatttt ttacgtatac tttcgatctt ctaattcgtt
     4141 ttgctaacca tcataaatgt tattaaccat catgacagca tcaccaattt agtccttctt
     4201 tactaaacct caacattaaa atgtgttttc aacagtttta aagactgaac cgtataatgc
     4261 ctaatttgta accccatttt aaagtcagta taagtttctg gcatatcgat aaatgtatta
     4321 aacattgctg taaccccttg tgattcaaat aaatcaaact tttcagcagt tgcacaaatt
     4381 gcaatggcaa ccttttgatg tttatgacaa agttctgcaa tacgcaatgt tgtcgtttct
     4441 agcaactgat cattttcatt taatccttct ccaaaaataa ttaaatccgc ttgttcaact
     4501 aaattttcta aatgtgttag ttggtctact aatgcatgac tggttaatat ttcagcttga
     4561 tacagtccat tcaagactgc tgcaattcca ccaccagcac caccacgttc aactggacca
     4621 attgcaattt tcaattcact tttaaataac tcactaaaat accaaattaa attatcgatt
     4681 tctgctgctt gattatgatt caactgatac gcatcataag tttgcatgat ttcactttgc
     4741 ttaccatata atcgacttga aaaatccgac attacttgaa ttcttgctgt ttccatttta
     4801 gggtgtaagt tcgacatatc catacgacga atatatttaa ttacaccagc accttgtctc
     4861 atatctacga cacgcccttc gtcatcatag aattgagcac ctaatgcttg taacatacct
     4921 gcaccagcat caaaactatc aatcccacct agtgaaatta caacatgttt tgcgtcgtta
     4981 tctaatgcat gtttaatcat ttctcctaaa ccataacttg ttcgttcaac aattggtttt
     5041 ttacctttta aaaataaatt cccctcgata acggtcatcc ctgtatcagt ttgtccgtaa
     5101 acaccttcaa cttcattcat atctgcatca tgtactggta tacgatactt ttgcccagat
     5161 tgccataaaa atacagaatc taataattca tgtcttccat taaacaatgg tacttgaact
     5221 acatctgcag tttcaatttg gcttgcaact gcctcttcaa catatctatt agcttgataa
     5281 cttgaaataa ttccatgaaa ctcatccatg gctactaata ctttcataac cttgaccacc
     5341 ttactttatt atttgttgtg atacgttttt tattcataaa agattaatct taaattttag
     5401 attttatctc gtgattatat gcgaacattt aacatgacat gattattaat tattccagta
     5461 aataacaata tgcttctttt ggtttagcat atatttccta ttaaactaac ttacaaatca
     5521 tgtttaaatt ttcttattgg attcgtcgtt ttccaaaata ttttcaataa aaatactcga
     5581 atatattata catatgtttg taaaaaagta ctaacttaac tgcttctaaa ggtgacataa
     5641 aataaaaggt cagaacatca ttatagccaa actactctga ttcacacgaa tttaagttgt
     5701 gctattcacg tctagtagtg taaggcaaaa tgttccaacc ctatagtttc attattcttc
     5761 tgcttgtgct aatgacgaca cattgatatc accgtggtct cgattccaaa ccatttcacc
     5821 atttacaaaa taaaatgctt gaggtgattc atgtttaacg ttcgtttttt tagcaatata
     5881 atctgacaaa tcgcgttctt gttggacaat caaataataa ccgtccatat cgcgttcata
     5941 taaaaattta ttaaattgat cgtacgcatt tgccgatatt ggacaagttt cactatgttt
     6001 taatacaaaa acatatttat tttcctcaat aacctgttca aattggtcaa ttgaacttag
     6061 ctttatagcc actctattca cctctaaaaa tatttatcac atttaaatag catctgcgtg
     6121 acatttttat acatatagtt aatatcaatg tttaccatta tacacatagt aagttgacat
     6181 gttaaccata aattcaaaat ttttaacttt gaaaaatgat tatagttatt tttcttctaa
     6241 tttttcaagc tttttttcaa aggctttatc gccgtgagtt atatctatac cctttttcgg
     6301 caacttatct acatcgactt ttgataactt ctcactaacc tttattgttt caatacgtgt
     6361 attataatag ttctgcagac tgtaatacat ttctattgcg tttttccttg cattattaac
     6421 atgcttatca ctaatattag tttggttaat atcggtaatt tgcttttcaa tcattggcat
     6481 aatgtgattt ttaatcgcgc cttttacttc attttcttta ctatcatcta ggttcttctt
     6541 cgcagtatct cttaacgctt tattattatg ttctaatttt gtcgtaagat tagttgcttc
     6601 acttttatta tcagctaatt taatttctga ttcaactttg tatctatttt tttcaaattg
     6661 tttcgtataa tctaaaatgt cttcgttata cttgatagat tgattacata aacctatgaa
     6721 tttttttaat tgatatatcg catccttctt ctcatttgca agcgtcatat attctttttt
     6781 taatttctta actttcattg tatcatcagg caaatttttt gctgacttat aatatgcttc
     6841 aaatttaggt atgagatggt ttttaacatc ttcttgtagt gccttaaatt ctttactatt
     6901 tttatcagtt gtatcagttt tacttagatg atcaatttct ttcaaatgta tattatccat
     6961 gacatgttgt aattcttttt gtttttgctc aacatcttta aattgcttag taaattgatg
     7021 ttgtgcctct ttttctttat taccacaagc acttattact acaaataaaa tcgctaaaac
     7081 agcgataata acaatttttt tcatgattgc actccttaac agattcatta tacaaaaaca
     7141 tgtttattat gaaaatgaat tatgttatag tattccagac tttatcgtga ttaaattaat
     7201 tttcaatatc aaaggagttt atcatgtatt caaatgttca accattcatt aatagtgatt
     7261 taactaattg ttttcaatca caatacactg aaattgaatc attattattc aacctcttag
     7321 acagcccagt aaaatataca aaacatattg gcggtacgca tcattttaaa tattcaacag
     7381 agccaatttt agatatttta gtaggtgtag aaaatttgca tgatattaca tccttagatg
     7441 aaaaacgctt aaattatgta gggttttatc gtttgcatca taaatatact aagaaagtta
     7501 tgatggcgaa gtttaacaat tttcatgaac taaaacaaca ggtgcgtcta catatcattc
     7561 aaatgaatac ccaaacattt gaatcttatc ttgcacttga ttacctctta tcaactaatg
     7621 atgatattgc tttgcaattc aatacaaaaa aacaccaaat attgcaacat agcttaacaa
     7681 ttagacaata tgagatgcaa aagcaacaat actttgaaaa actatgtaaa aaatacttat
     7741 aacgacaata atgccgtatt ttataattat actaattcta atactttctt ttcaattttc
     7801 gcaaatgaat tttaaaattg gtataatact atatgatatt aaagacatga gaaaggatgt
     7861 actgagaagt gataaataaa gacatctatc aagctttaca acaacttatc ccaaatgaaa
     7921 aaattaaagt tgatgaacct ttaaaacgat acacttatac taaaacaggt ggtaatgccg
     7981 acttttacat tacccctact aaaaatgaag aagtacaagc agttgttaaa tatgcctatc
     8041 aaaatgagat tcctgttaca tatttaggaa atggctcaaa tattattatc cgtgaaggtg
     8101 gtattcgcgg tattgtaatt agtttattat cactagatca tatcgaagta tctgatgatg
     8161 cgataatagc cggtagcggc gctgcaatta ttgatgtctc acgtgttgct cgtgattacg
     8221 cacttactgg ccttgaattt gcatgtggta ttccaggttc aattggtggt gcagtgtata
     8281 tgaatgctgg cgcttatggt ggcgaagtta aagattgtat agactatgcg ctttgcgtaa
     8341 acgaacaagg ctcgttaatt aaacttacaa caaaagaatt agagttagat tatcgtaata
     8401 gcattattca aaaagaacac ttagttgtat tagaagctgc atttacttta gctcctggta
     8461 aaatgactga aatacaagct aaaatggatg atttaacaga acgtagagaa tctaaacaac
     8521 ctttagagta tccttcatgt ggtagtgtat tccaaagacc gcctggtcat tttgcaggta
     8581 aattgataca agattctaat ttgcaaggtc accgtattgg cggcgttgaa gtttcaacca
     8641 aacacgctgg ttttatggta aatgtagaca atggaactgc tacagattat gaaaacctta
     8701 ttcattatgt acaaaagacc gtcaaagaaa aatttggcat tgaattaaat cgtgaagttc
     8761 gcattattgg tgaacatcca aaggaatcgt aagttaagga gctttgtcta tgcctaaagt
     8821 ttatggttca ttaatcgata ctgaaacacg ctgtcgccat tattttaccg aagaagatat
     8881 tattgctatt aaatttaaat gttgtaataa atactatcca tgctataagt gccataatga
     8941 gtttgaaaag cacgccatta agcgttggtc tgagccttca tttaacgaaa aagcaatctt
     9001 atgcggtgta tgcaaacacg aattaacaat caatgaatat atgatggtag agcgttgtcc
     9061 aaattgccaa tctcggttta acaatcgctg taaatatcac tatcatattt attttgaaat
     9121 ttaaacaaca tagtcaaaac attataggga gtgggtcaga catgataaag caccacttct
     9181 gtagaattcc tttttgaaat tctctatgtt tgggccccgc caacttgcat tgtcagtaga
     9241 aattgaaaat caaatttctc tatgtttgga gcccactagt attgaaatga acttgttaca
     9301 agtacatgtt cattcaacca acgactgaca acagaacatt ttagagtcta ggacattgat
     9361 tgacgtccca gatttccttt tttaaagaaa agaggggagc aatgattaag acctttaacc
     9421 attgttcccc tcttttaaaa ttattttaca actttatcaa gttcctcaat ttgtttaatt
     9481 gtagttgtag ttgatccagc tgcaaagtac cataatttag gatctaaatt atatactttg
     9541 tcttctttaa ttgctttaac attttttaat acaggattat ttaatgcttg tttcgcagtt
     9601 gatttaccac ttatcgcttg acctctatcc atcgctaaaa taacatctgg attttcttta
     9661 ttaacatatt cgttagaaac attttgtcca tgattgctat tacttacttt tttatcaact
     9721 gcattgaatc ctaatgtatc gtaaactaat ccaccaaaac gacctttagg tccaaatgtt
     9781 gataattcac cttcgttaac tagtaaatac ataacagttt tattgaagtt tttcgtttta
     9841 tctttcattg aagcaatttt gttatctaaa tctttattta attctttagc tttatcttct
     9901 ttatcgtaaa tttttccgat attttcagtg ttttgtttca ttgaaccaat taagttcttt
     9961 tcatctgcac caacataaac aattttcgct ttaggtgcag cttttttgaa ttcatctaaa
    10021 ttcttttgat tagctgtacg tccagagata aagattactt cgggtttcgt cgcagcaatt
    10081 ttatcaaaat tcacttcttt taaattacca acgtttgtat atttatcatc tttaaatgat
    10141 tctaagaaat tcggtaatga cttaccgcct tcccctttag gtaatgcttt tactttatct
    10201 gataagccca tttctttcat tacatctaat gcgccatagt ctaacacaac tgcattttta
    10261 ggattttttg gtacttcaac agtttcttta acttttttag cgtcactacc atcttttttc
    10321 tcgccacgca ttttatagtt attttcaatt tttactgtat ctttagaacc tttagtttct
    10381 gattttgatt gttctttatc agaattgtta ccgcatgccg ctaataaaaa cattactgct
    10441 aatactaaat ataagactgt tttcttcata atataatatt ctcctctttt ggcttaatct
    10501 gtgtgcttga tgtgaaatat aaataaatgc tttacgttta acccttcttt aattggcgca
    10561 tcaccccttt aaagtgtctc caaattatca atccttaaaa tataaacaaa tcctttgatt
    10621 tcttatatgt tcaatttgaa tattcatgtc atataaatca cttaaaacac tactttgaat
    10681 gacattatct ttcaaatctg acttaactag ttgtccgttt ttcaatgcta caatctgatc
    10741 tgaataacag gacgcaaagt taatatcatg taacacaatg acaatcgctt tattcatttt
    10801 atgcgctaac atttttaacg tttgcataat ttgaacagca tgcttcatat ctaaattatt
    10861 taatggttca tctagcaaaa tatattcagt atcttgtgct attgtcattg caatgtatgc
    10921 acgctgacgt tgtccaccag ataatgactt aatattacga tttctgatat cttgtagttg
    10981 caacaaatct agcgcatcat tgacaatatc atgatcctct ttcgtcaaac gacctttaga
    11041 ataagggaat cgtccaaagt ttaccaactg ctctaccgta atattcattt cagtatggtt
    11101 tgtttgtttt aatatagata tttttttcga caagtcatta tttttataat cagacatgag
    11161 ctgtccatct attttcactt caccgttctc aaaacgaatt aacctacata tcgctgaaag
    11221 taaagtactc ttacccgcac cattaggtcc aattaaagaa gtcaatttac ctttttcgat
    11281 atctatgcta atatcttcca atatcatttg attatttata gttttagtta aattttcaac
    11341 ttgaatcatt gcgcatttct ccttctaact aatagataaa tgaaataact tccaccaatc
    11401 aaatcaataa gtatactcat ttctgtcgta gcttcaaaca cattttcaac tacccattgc
    11461 gcactaaata aactaatcca actcaagcaa attgtcgcaa ttaaaatata cttatgttca
    11521 tacgtcttca ttagttcatg cgctagattt acagttaata aacctaaaaa tgtaattggt
    11581 cctactaatg cagttgacac agaaactaaa actgagacta ttacaagtag aattcgcgtt
    11641 aatttttcat acgatatccc aagattaatt gcttcagcac gacctaaaag caatacatct
    11701 aaataaggca atagtaaaat tgtaatgact aataagatta ctaatagcac tgctgagaaa
    11761 gtaactaaat tcgaattaga agcattaaaa ttagcaaaca tactactttg tattgctaaa
    11821 aatgactcag gatccataat cagttgaata aaacctgtta tgcttctaaa aaatgttcct
    11881 aaaaggacac caataagtaa gataaaataa attgaaaaat gtccgatttt aaagatacct
    11941 tggaatagga ttagtgcgaa taacaccatc gttataagtg ttaatataaa gttcaaatat
    12001 acattagtaa cccataccga ttgaattcca aataaaaaga ctggcaatac tttgataaat
    12061 aaataaactg catctaaccc cattattgat ggtgtcaata gacggttatt tgtaatcgct
    12121 tgaaaaatca ccactgaagt tgcaatggca gcacctacta aaataattaa tatgaacttt
    12181 cttaaacgac ttgaaaattg atattcaaat atttcaaaat caatacctac aaataagtac
    12241 agcatactaa ttaataacgt tactgcaatt aaaagcatta tttttttatt attgttgcgc
    12301 ataatttttc ctacctttca taagcaagat aaggaaaatg attgttccaa atacacctat
    12361 tgttaaacca atatttattt catatggata aacaacaatt ctgccaacta tatcagaaaa
    12421 taatacaaag atggcaccta acatcatcgt atgagggata gcatttttca aatgatcacc
    12481 tcgataaatt gaaataatat ttggtattac tagtcctaag aacggtaatg ttccaacagt
    12541 caccactacc aatgctgtaa tagttgcagt aataaacaat gcgatgttaa ttaatttttc
    12601 gtaactcaaa cctaaattat tagtaaagtc tttacccatt cctacaatcg tgaaatgatt
    12661 agcaaaaaga tatgtcaatg ctaaaagagg aatacttaaa tataaaattt catagcgtcc
    12721 acttgtgata atggcaaagt tcccgttaag ccagttacca atgctttgaa cagcattcgt
    12781 gcgcaatgag atgaaggttg tgaaacttgc aacaatccca cccatcataa ttcctaaaag
    12841 cggtacaaaa atgacatctt tcacttttat aaaatcaatg attttaacaa ataaaaacgt
    12901 accgcaaata ctgcaaataa cagcaaatac tagttttaat aaaatatgac cggttggaaa
    12961 gaacaataaa gcaattaaaa tacctagttt agcccattcc atcgttccag ctgtagtcgg
    13021 actaacaaac ttattttgca tcatttgttg cattatcaag cctgctaaag ccaacgaact
    13081 tccagcaatt aaaatactca tcgtccttgg tattcggctt gagaataaaa tattccgctg
    13141 tgactcagtt aaatgtagta aatctttaat tgatagttca ctcacaccta tgaacaacga
    13201 aataattgtc aacaatatta gtaatagaag caaaatattt ccctttaata aa
//
LOCUS       DALAAR010000066        12603 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000066 DALAAR010000000
VERSION     DALAAR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.068"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            341..544
                     /locus_tag="O2458_002090"
     CDS             341..544
                     /locus_tag="O2458_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887945.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            727..1662
                     /locus_tag="O2458_002091"
     CDS             727..1662
                     /locus_tag="O2458_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCZ3887946.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(1744..2712)
                     /locus_tag="O2458_002092"
     CDS             complement(1744..2712)
                     /locus_tag="O2458_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3887947.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2845..3189)
                     /locus_tag="O2458_002093"
     CDS             complement(2845..3189)
                     /locus_tag="O2458_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ3887948.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(3231..3641)
                     /locus_tag="O2458_002094"
     CDS             complement(3231..3641)
                     /locus_tag="O2458_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ3887949.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(4021..6087)
                     /locus_tag="O2458_002095"
     CDS             complement(4021..6087)
                     /locus_tag="O2458_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ3887950.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6388..8127)
                     /locus_tag="O2458_002096"
     CDS             complement(6388..8127)
                     /locus_tag="O2458_002096"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCZ3887951.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(8174..8863)
                     /locus_tag="O2458_002097"
     CDS             complement(8174..8863)
                     /locus_tag="O2458_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCZ3887952.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8856..9251)
                     /gene="cidA"
                     /locus_tag="O2458_002098"
     CDS             complement(8856..9251)
                     /gene="cidA"
                     /locus_tag="O2458_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCZ3887953.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9464..10342)
                     /locus_tag="O2458_002099"
     CDS             complement(9464..10342)
                     /locus_tag="O2458_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3887954.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10509..10727
                     /locus_tag="O2458_002100"
     CDS             10509..10727
                     /locus_tag="O2458_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ3887955.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            11116..11547
                     /locus_tag="O2458_002101"
     CDS             11116..11547
                     /locus_tag="O2458_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3887956.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11812..11916)
                     /locus_tag="O2458_002102"
     CDS             complement(11812..11916)
                     /locus_tag="O2458_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887957.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(12079..12213)
                     /locus_tag="O2458_002103"
     CDS             complement(12079..12213)
                     /locus_tag="O2458_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887958.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
     gene            complement(12307..12564)
                     /locus_tag="O2458_002104"
     CDS             complement(12307..12564)
                     /locus_tag="O2458_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887959.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
BASE COUNT         3955 a         2327 c         1831 g         4490 t
ORIGIN      
        1 acagttgtgt gacagttttt tgataacttt tttacatctg aaagtaagta atatttctaa
       61 aaacttttaa tatttataca ctttatcttt cgagctattt attttttgtt tttgtgctgt
      121 gtttataaaa ccgcacacta tattcaaaaa taacttatta cacatatatt tgcaataaga
      181 taattaaagt aggatattat ttttagtttt ctgataggaa tgatgatagt ctataggttg
      241 aatctttact tttttttaaa gctaaattta catcaactta acaatggttg gttatactga
      301 agatgaaaat atttagtata acttagtgga ggcgataaag gtgcaattga gtcattccgt
      361 taaagttgca atttctatct atttagcact tatctttata acgttcactt cttatttagt
      421 cattatttta tatacgagta tgactggaca tgatgtatca catttcgtgt tagatagtca
      481 gcattctcat catggatctc ttacgcaaaa acatttgagt cttcctgaaa tctcatttaa
      541 atagtttatc ctctgtgttt caacattcat ttcccatatc gattcattta tctatcatct
      601 agaccactac atcttagatg atttttttat tttctcattt cactctttct taaaagtcga
      661 tataatgaat taaatcatta tcatacaccg acatatttta tgttgttggt gttaagtttt
      721 aaaggggtga gatacttggc gaataatcat tcagctttgc gtttaaaagg aattatactt
      781 gccattgtcg gtgcttgttt atggggatta ggtggtactg tttctgattt cttgttcaaa
      841 tataagaata ttaatgtcga ttggtacgtc actgctcgac ttgtagtcag tggtgttttc
      901 ttacttatta tgtacaaaat gatgcaaccc aaacgttcaa tatttagcgt attccaagat
      961 cgacgtatgt taggcaaatt acttatcttc agtatactgg gcatgttagt agtacaatat
     1021 gcttatatgg catctattaa tacaggtaat gctgcgattg caacattact acaatacatt
     1081 gcgccagttt atattattat ttggtttgtc ataagaggcg ttgcaaaact aacattattt
     1141 gatgtgcttg ctattatcat gacactatta ggaacatttt tattattaac aaatggttca
     1201 ttttctaatt tagtcgtcaa tcctgcaagt ttattctggg gtattttagc tggtgtagca
     1261 ctcgcttttt acacaattta tccttcagac ctacttaacc gcttcggttc gattctaatt
     1321 gtcgggtggg caatgcttat ttctggtgtt gcgatgaatt tacgccatcc aatttggcac
     1381 attgatatca ctaaatggga catatcaatt atattatttt taatctttgg tattatcggt
     1441 ggtaccgcac tcgcatttta tttctttatc gacagtttac aatacatatc agcgaaagaa
     1501 acaacattat tcggaactgt tgaacctgtc gtagccgtta tcgcaagcag tctatggtta
     1561 catgtggcat tcaaaccatt tcaaatcgta ggcatcattc ttattatgat tttaatttta
     1621 ttactatcac ttaaaagaca acctgaaaca ttagatgaat aagaaaactc tgataatcac
     1681 tttagcaagt aactattatt taacaacgta gttaccttat aggtgatatc agagtttttt
     1741 attttagtta ataatatttt tcacttggta taaaaagcgt cgtcgctctg gtatcggaaa
     1801 tactggataa atatggaatt ggtaatatcc aggtataaag tcatgttcga tacctttcgc
     1861 actcaacaat tgcgataagt tcaaagcatc tgggtacagt acttcttttg taccaacagt
     1921 taatgtaata cgacctaaac catctaagtc accattaatt ggtgatactt tgtagttatc
     1981 taaaggtgtg tcccctgccc attgttcagc taagaacaca ctgccatcca cacctaccat
     2041 tgggtctttc tttaagtagt caggaatttc aggatgctgc atcgttgcat ctaaaactgg
     2101 tgaaattaat acaatatgtc ctggttgcac aatatgtttt tcttttaaca attgagcaaa
     2161 tgataaagca atttgaccgc ccgcagagtc acccattaca acgatttgtt tagaatctgc
     2221 tacttgattc aataaatcat ggtacaactt ttcaaaaagc acatacgtcg cttgataatc
     2281 ttgatgcgga atcttcggat atactggcat gatgacttta gcattgagtg tttctgcaag
     2341 ttcatcaata aattcgaaat gaattttgag tgggtcttgg aaccatgcgc ctccatgtgc
     2401 atataataca acgcgttgat gcttatcttg tttatcgtta acggtataaa cggttgaacc
     2461 gaagtgctta tccactttta ctggcatctt aaactgataa ttggttccct catattcgcc
     2521 tgctgtctgt ggcgccattt tttctaatgc tttcatcgct tcttctttat acataaaacg
     2581 acgcttagct ctagataatc gtataccttg ttctaacata taacttttca cactgcgttt
     2641 ttctttaatt ctaatatgtg cgtatgctgc aactaaaaat ccaaacgcaa gtgtagacca
     2701 ttttttacgc atttgtcgtc actcctcttt gatgatttct actatagttc tactgtacca
     2761 tgctgattct aattactacc aatatttact gggaaataag ttatgatgtc tacataatct
     2821 tagcaatgct aatcatttgc gatattaatg ctcatcgaca tcgttattta cactatcatt
     2881 catttgctta agatgatgtt caaaacgttg catatcgata aaacgccctt gacgatgcat
     2941 ttcttttcca ttcataaaaa ttaaatccac aggtacagtg aaaatattta attctccagc
     3001 aatagcttcc acctgacttt gattaatcac agctaatggc acattgggat atgattgcat
     3061 caagtcttca atttgtggta aaacggcatg acacacgcta cactgatcgc gcatgacatg
     3121 tacaactgct aacggatatt gatgaataaa ttcacggaat gcttcgacac tatttaattg
     3181 cttaaccatt tcgaacacct tcttactttt tatattttaa aatgaagtca ttattttgtt
     3241 gctaaaatgt cttgaaacac ttgtggcaca gaggttatct tcttatcact caatctgtaa
     3301 aaaccgcctg tctgtgtacc aatacaatgc acgccatgtg cggacacaat ttcaaactga
     3361 aacacccatt tcatcttgct tgctttaact aagtaaatac gacctactgg tttgtcatgt
     3421 attgtgacag gtactttata ctctgcttcc gttttcataa gtataggtga aatgccttga
     3481 ttgatcattg ttgagtaatt catgtgctga ttaataaacg ctgttctcaa atcttcgaac
     3541 catctaatat aaacgatatt actaacgaca cccatcgcat caatgtcgta accattcact
     3601 tcgatttctt tctctgcaac taatagacct tctgttgtca taatgacaaa tctccttatc
     3661 ttacaaatag atttaaaatc atgttaattt atttttatta ctttcatgat aaaaatacaa
     3721 gcaattgaac tttattacgg aaaatcaaac ttttcaatga gactattaat ttggctagtc
     3781 gcgaagatgc atttttctaa aatgtgacaa aaacataagc aatgttttct atatctaaca
     3841 ctaatattta acttattatc atcaacacac ctgctataca tcattgcgca ctaaaaaggc
     3901 agtagcttca ttttaagcta ctgccttttg taacgtattc tctttcaact cgctacttcg
     3961 catacgcact acgaatgact aaaagttcat tcatttgata tgctgtttca tttttaatca
     4021 ttatttgact gtcataatta gcttacctgg atcagcatct tgtacatctt caatgacaag
     4081 ttctttattt tcaagatttg taataatcat tggtgtaacg atgcttggtg cgtgtgcttt
     4141 caagtatgct aaattcactt tcattaatgg ttgaccttgt gttacttttt catcaacgtt
     4201 aatcagactt tcgaatcctt caccattcag tttcactgta tcgataccaa tatgaataag
     4261 tacttcgacg ccactttcag attctaatcc tatcgcatgt ttcgtaggga agattgtttt
     4321 cactgtacca tcaaacggtg ctacaatttc acctttttca gggataaagc caacaccatc
     4381 acccatcatt ttaccagcga acacttgatc aggtacttct gataatggaa tgatttgacc
     4441 gatacctggt gcatagatgt ctgttgttcc aagtgcttct acgtgaactg gttcatctga
     4501 catttcttca gtcactgtcg tttcacttgg tttcgtaatt tcaccactca taatcttggc
     4561 catatcatgt ttaatttgat ctgattttgg accaaagata gcttgcatat tgtttccaac
     4621 ttctaataca cctgatgcgc ctaaagcttt aatacctgct acatctactt ttgatttatc
     4681 aaccacttct acgcgtagac gtgtaataca tgcatctaaa tgtttaatgt tttcttttcc
     4741 acccattgca tctaagacat caaatggtaa ttttgcgaca ctagagttac gaatttcagt
     4801 ttcttcatct tcacgacctg gtgttttcaa tttaaactta cgaattgcaa agtcgaataa
     4861 gaagtaatac acgatagcat atacaatacc gactggaata actaataatg cgtgtgaacg
     4921 atcccagttt aataaaccat ataaaatata atctatgaaa ccacctgaga atgtcatacc
     4981 aattttaacg cctaataaat gcattactaa gaatgatgta ccagctaata atacgtgaat
     5041 tccataaagt actggtgcta caaataagaa tgaaaattct aatggctcag tgataccagt
     5101 taaaaatgca gttaatcctg ctgataacat taaaccaccc acgacttttt tacgttctgg
     5161 tcgtgcattt ttataaatag caaatgccgc cgctggtaaa ccaaacatca taaatggata
     5221 tttaccagta gtaaatgcac cagcagtaaa tggtacgcca tctttcaatt gtgccatcca
     5281 aatacgttgg tcaccacgaa ccaattcacc tgcgtgattt gtataacttc cgaattcaaa
     5341 ccagaacggt gaatagaaaa tatgatgtaa accaaatgga attaatgagc gttcaataat
     5401 accgaatata aacgttgtta atgttaaatt tttatttaat aagaaattcg ataaactatt
     5461 taatccatct tgaattggtg gccacgcaaa gcttaaaagc acacctgttg cgattgctac
     5521 gaccgatgtc acaatcggta caaatcgttt acctgcaaag aatcctaaaa atggtggtag
     5581 tgtaatatta taaaatttgt tgtaacacca tgcggctaaa gcacccatga taataccacc
     5641 aaacacaccc gtttgtaacg ttggaatacc taatactaaa gcatgtgctg gttctttcgc
     5701 tgcttgactt aattcttttg cccctttggc atatgagaaa atgtcatcaa ttgtaatgtg
     5761 caacactttc cccattgttg cattcataat taagtaacct actagcgctg ctaatgctgc
     5821 aacaccgtct cctcctgcta atccaagtgc tgtaccaact gcaaataata atggcaagtt
     5881 atcaaataca acttgtcctg ctgcttccat gaccgacgaa atcattacaa tgatatcgtt
     5941 ttttaaccat ggtgcaattt ctactaattg ttcgttgtgc attgcgttac caaacgctaa
     6001 taaaatacca gctgctggta aaatcgcaac aggtaacatt aatgctttac cgatacgttg
     6061 caattgtcca aacaatttct taaacatata aacatcccct atctcaattt ccgcttccat
     6121 gtacaaaaaa agcaggcatg aacaaacaag actattctca tagtgcggtt gtttgctcat
     6181 gcctgcatta ccagtaacac gcaagtgata tctaattcat agctattata catatttgta
     6241 aaagcgtttt caacaacttt ttcacaattt tatcatcaat tcagtacgat catttgtgat
     6301 aaataaacaa aacggcaatt cccattttgt tgaaatagaa attaccgttt ttatagttca
     6361 attcaattgt gataaccttt aaatcagtta taagaaacgt tttgctgcca ctgaaattgg
     6421 tggaatttga tctaaatcta aatgtttatc ttcagtaatt gatctaaatg cccacttacc
     6481 ataaccaagc gcttcttcat ttacaatttt acctggtaat ggcgcagcat taggatcaac
     6541 atatacatct acaatcgttg gtacatcttg tgctaatgcc tcttcgacta tagcatctac
     6601 ttcgctagca ctcttaattg tataaccttt accacctgct gcctcagcaa attttgcatg
     6661 atccatatca gaaaaatcaa ctgcatattc taattcacca gctgcttgtt gttcatattt
     6721 aataaatgct aactgtttgt tattaagtac aaatacagtt aaaggtaaat catattgtac
     6781 tgctgtagcg aagtcttgca ttaccatttg gaatgcaccg tcaccagcaa ttgcgatggc
     6841 ttgtctattt ggatatgcaa tttttgatgc aattgcacct ggaagaccgc aacccattgt
     6901 acctaaccaa cttgaaatga tgaacttgtt atttacacca aggtttaagt atcgagttga
     6961 ccaaactgtt gctgtaccta catctgctga aatcactgca tcatctttaa taaatttatt
     7021 gattgatgcc attaatcgtt ctggacgtaa tggtttacta ttattatttt tatcttgttc
     7081 catccattta tcccaaaccg ctttacgttc taacgttttg tttaagaatg gtctttcagc
     7141 aacatgttta atattttcag ttaactgatg caacgcaatt ttactatctc caacaattcc
     7201 tacattaata ttgaaacgat gtccgatatt tttaggattt gtgtcaattt gaatggcttt
     7261 aatatttttc ttaggtaagt aatccacata tggatagttt gtaccaacca taattaataa
     7321 atccgcttcc tgcattgttt gataagatgt tttggtaccg attttaccta agttaccgat
     7381 actatatgga tgatcatccg gcaagattgt tttagctggt aatgaatgaa tgacaggaat
     7441 tttagccatt tcaataaatt cacgtagctc atctttcgca tgtttcgcac ctacaccaat
     7501 taacatgaca ggctttttac ttttattaat tagtttaacc gcttttttga tgtctttata
     7561 ttttggtgat actactgttg gtcttgatgt atctactggt ttattcgttg tatctttaat
     7621 tttttcagtt aataagtcgt taggacaaat aacaacagct acaccttttt gttcatatgc
     7681 cgtacgaatt gcttcgttaa cgatttcaaa cacattgtca cctttttcaa tttggtgatt
     7741 ataaacggct acatcttcac ataatttttg taaatttgtt tcttggaatg ctttcgttcc
     7801 aagtgctgta ctattcgttt gtccagataa tattaattgc ggtacattat ccattttggc
     7861 atcatacata ccatttaata aatgaattaa accagggcca ccgatactta atgccacacc
     7921 gattttacca gttaattttg tgtaaccagc agccgctaag cttgctactt cttcatgacg
     7981 tacatgataa aatttaaatt gatctctcac tgtacgtaaa ctatcgacta ctgcgtcgat
     8041 tgagtctcct ggaataccat acaagtgatc tatatcccat gcttgtaatg ctttaactaa
     8101 tgcttcattt gcttttattt ttgccattat tactaatagc ctccctttct gtctatcatt
     8161 ttaaatatct aaattaatag aataataaaa ttagaacagg tattaacact gaactaataa
     8221 ctgcagttaa aatcatccca attgaactaa atgcaccgga ttcaatatcc atttctagtg
     8281 ctttagctgt gccaaatgca tgtgacgcat tcccatacgt taatcctttg gcgatagaag
     8341 attcaaatct tccaaatctt aataacatcg aacctaaaat actaccgatt aaacccgttg
     8401 tgataataaa aagtacggtc atcgtatctg taccacctag ttcatgtgac acttcgatac
     8461 ctactgcggc tgttatagat cggggcaata acgttacaat gacgtcttta gaatagccaa
     8521 atgctttaag tgttaagaac actaacatga aattcagcat gacgccagtt aatacacttg
     8581 caaaaatgat agagacattg tctttaattt tctctctatt tttataaagt gggtacgcta
     8641 aacatacgac cgttgcgttt aaaatatggt tgatccaact gccacctttc atatacccgt
     8701 tataactaat tccaaagata agtaagacaa aaataattcc taaagatgca attaatgctg
     8761 gattcaaaaa tgggttcgga tatttttgtt gtaacctttt agcgaaataa tataaaacga
     8821 cagtcaacaa aatcattaat aaggcttgca cgtaatcatt cataagcgtc tacaccttta
     8881 cgatgtttat gtttaacaga cattttttca gcaatataac ctgaagataa tgcaacgata
     8941 catgttccta tgataatgac tgcgaaaaag agtatataat ttagcgtaat ttcggaagca
     9001 acatccataa tacctacaac tgacggtatg aagaaaaaga ccatcgtctt taataaaaag
     9061 tttgcaccgt cttctaccca agttagcggt acaatcttaa attgtaatag taaataaaat
     9121 aaaaatagac caacaatact gccggctaag ggaagatgaa aaatcttttg aatttctgtg
     9181 ccaatataag taatcacaat gatgattcct agttgtagta gtagtttgat tattaattgg
     9241 actttgtgca tggcgccatc cctttctaaa tatgtctaaa ttgttacaat aactattata
     9301 aagatggcgt acacaatata aaaatactct ttttttatag tttgtattac tattttctat
     9361 ggaaacgctc tctaaatttc acttcatttt cacaaaaaaa cactcattat ttcaatgacc
     9421 cacgtaatga tgcgccttaa ataatgagtg ctttattaat atgttagcct aaacggtctt
     9481 tcaaaaattc tatccatttc gttgtggcat gacttaattg tttatccttc ttccaaacaa
     9541 cacctaattc ccaatgtaca tgagcatctt caatgcgtag cagcttcaca tctccattta
     9601 gttgctctga aattgatgtt ggtaaaatac taatacctaa ttcattcgta actaaatctt
     9661 ctataacatg ccattgtgaa atttgcgcta cagtattcgg aacaaagcca acgtttttag
     9721 cattttcaat aattttatca ttcaagtaaa agtctttatt aaataaaatg aagtcttcac
     9781 ctgctaaatc ttcgagttta acagtttcat attttgcgag tcgatgctct ctgctcacga
     9841 taagtcgcaa atcttcctta tctagggtag tataatcgaa aatatgatga tcgactggca
     9901 aagtggtcac gcctatatct acttcatcat tgataatttg ctgttcaatt gttttaccgc
     9961 cattttctat caaattatat gtaacatttg gatattgttg atggaatgca ccaagaatat
    10021 tgataagaat cttcatattc atgactgccg acatgcccat gtttatatgt cctgtttcca
    10081 gtccattcaa gcgttccatt tcagatggta aataatcata cagtgcaaca atttctttac
    10141 ttttctcata aaaaatttga cctgcatcgg taagaattaa atgtctttta cttctatcaa
    10201 ataagggcgt gcccatttca ttttcaatat ctttaattgc tttactaatt gtaggttgtg
    10261 caatatataa tgatttggaa gcattagtca tgcctccttg cttaacgact tcaataaaat
    10321 atttcatatg tttgatatcc actaacatca tcctaactat ttcaacacgt ttatttcatt
    10381 atacacaact tcttaaacat tcaataaatt tagatttata aaattgcttt ataaaaagtt
    10441 ttatgtaaaa tacacattct tttatggtat tatattaatg gctcaagtat attaatcgag
    10501 gtgaggtatt gagcttttac gaatttatgc aaagttttct tggtgatgac acaccattag
    10561 gtgaattggt tgattggatt aatcaagata gcaatttccc tagagaagtg aagagccata
    10621 gtgaaatatt gtcatatttt cgaacaaatc catgtcctga aagcatctca gtacctgtaa
    10681 ttaaacgggc actatcagtt ttcaaccaat tcacaaatgt acaatgatac atgaatacat
    10741 ggagtcacat catctcatgt tagcgctctg attgcagggc gctattttta atgttattgc
    10801 tacatttagt atattgtgat tgctattttc tttcagttat agaaccatgt accttttttt
    10861 taaatcattt tatattttcc cactaatatc cgctgttaat caatcctgac atccttgtat
    10921 cactatgaca attaattgtt aaatacatga atttctacat tttatgaaaa aatccatttt
    10981 tattacaatt caacacttta tatgacaact tcattacagt tacttttatt gttgattgct
    11041 tacattgttt tctaaaaaaa atttgttatc ataattaacg ttgaataaag aaaaaaatta
    11101 acttgggaga taaaaatgga atataaaaag atactaattc gtttattaat tgcttttgca
    11161 gtacttttct cagcagattt cacttatcaa tccgtcgaac aaacgcatca atcgcatgcc
    11221 gcagttaatt attatagtaa aaaccaatgt acatggtggg catttaaacg tcgcgcacaa
    11281 gtcggtaaac ctgtttctaa tagatggggc aatgctaaaa attggtatta caatgcacgt
    11341 aaatcaaaat atgcgactgg tcgtacacca agaaaatttg ctgtcatgca atcaactgca
    11401 ggatattatg gacatgtcgc agttgttgaa caagtatata aaaacggtag tattaaagtt
    11461 tcagaataca acttttatcg cccattaaaa tacaatacac gtgtactaag caaaaaggca
    11521 gcacgtaact ttaactatat ttactaatca aaaaacttct atcacgaacg cttcaatttc
    11581 ctgtatgcgt gtgatagaag tttttatttt atgaaattat attattactt ctacaaattt
    11641 caaattgccg taattgaacg tatatttctt cttcaactat tatttcatct ttagcataat
    11701 ctatatataa aattttatgc tattatttaa ataattcgct ataacttaac atacgttttc
    11761 gatataaacc ttgttctaaa tctcaataat tttttgctgt tttcatcgtc attagttaaa
    11821 aaaataattt aactgagttt ttgagacctg tttaaattga tgactcaaaa cctttatccc
    11881 cttttcactc ggtttaattg ctttaatatt taacacagtc tcattgtaat tttgctacta
    11941 agtttgaaat atttcgattc gaatgtgaaa catcatttat tattactttc gacagctaga
    12001 aaaatattgt taaaccaaaa ctataattac cacttttata tagaactata tataaatttt
    12061 aactttgaga cagaactgct agtcaggttt atgaatatat ttctttagtt tacttgatat
    12121 acttattggt aaatcattat ttatttgaag agcatttata acaaaaagta taatcccagt
    12181 tatagcgatt ccatgaccat ttttatcagt caaagattca tcaacaaaaa ctttttgata
    12241 aatttatata tttgtataat ttattatggt agatatctac acccgtatct ataacagcta
    12301 cagttatcat tgcgtcttat ccaaatagtt tttaagaaat aaatataatt cattaatatc
    12361 atggctattt gtaacgtcag tgcttaagac cttgtcaatt attgttttat ctgtttctaa
    12421 gagcccctga ttttctgcat acatattttg tatatcgctg ttaatctcat ttaatttttt
    12481 aaattttttt ctaacctttt tcaaaataaa atgctcttta attccatctc ttaaatacat
    12541 ataaatctct attataatct ccataaatgt catatctcct ctctcaaaaa acattatact
    12601 caa
//
LOCUS       DALAAR010000067        12526 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000067 DALAAR010000000
VERSION     DALAAR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.069"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            55..714
                     /gene="arlR"
                     /locus_tag="O2458_002105"
     CDS             55..714
                     /gene="arlR"
                     /locus_tag="O2458_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCZ3887960.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            711..2066
                     /gene="arlS"
                     /locus_tag="O2458_002106"
     CDS             711..2066
                     /gene="arlS"
                     /locus_tag="O2458_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCZ3887961.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2350..5148
                     /locus_tag="O2458_002107"
     CDS             2350..5148
                     /locus_tag="O2458_002107"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCZ3887962.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5162..6430
                     /gene="sucB"
                     /locus_tag="O2458_002108"
     CDS             5162..6430
                     /gene="sucB"
                     /locus_tag="O2458_002108"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCZ3887963.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7010..7819
                     /locus_tag="O2458_002109"
     CDS             7010..7819
                     /locus_tag="O2458_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ3887964.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            7848..8051
                     /locus_tag="O2458_002110"
     CDS             7848..8051
                     /locus_tag="O2458_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887965.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8232..9023
                     /locus_tag="O2458_002111"
     CDS             8232..9023
                     /locus_tag="O2458_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCZ3887966.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9037..10923
                     /locus_tag="O2458_002112"
     CDS             9037..10923
                     /locus_tag="O2458_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCZ3887967.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            11148..12491
                     /gene="brnQ"
                     /locus_tag="O2458_002113"
     CDS             11148..12491
                     /gene="brnQ"
                     /locus_tag="O2458_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ3887968.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         4625 a         1802 c         2234 g         3865 t
ORIGIN      
        1 caaaatttaa aatatattac cttattagaa aaagtcgtaa tatgaggtgt acaaatgacg
       61 caaattttaa tagtagaaga tgaacaaaac ttagcaagat ttcttgaatt ggaactcaca
      121 catgaaaatt acaatgtgga cacagagtat gatggacaag acggtttaga taaagcgctt
      181 agccattact atgatttaat catattagat ttaatgttgc cgtcaattaa tggcttagaa
      241 atttgtcgca aaattagaca acaacaatct acacctatca ttataattac agcgaaaagt
      301 gatacgtatg acaaagttgc tgggcttgat tacggtgcag acgattatat agttaagccg
      361 tttgatattg aagaactttt agcaagaatt cgtgcaattt tacgtcgtca gccacaaaag
      421 gatattatcg atgtcaacgg tattacaatt gataagaacg cttttaaagt gacggtaaat
      481 ggcgcagaaa ttgaattaac aaaaacagag tatgatttac tatatcttct agctgaaaat
      541 aaaaaccatg ttatgcaacg ggaacaaatt ttaaatcatg tatggggtta taatagtgaa
      601 gtagaaacaa atgtcgtaga tgtttatata agatatttac gaaacaagtt aaaaccatac
      661 gatcgtgaca aaatgattga aacagttcgt ggcgttgggt atgtgatacg atgacaaaac
      721 gtaaattgcg caataactgg attattgtta ccacgatgat tacgtttgtc acgatatttt
      781 tgttttgttt aattattatt tttttcttga aagatacact gcataatagt gagcttgatg
      841 atgcagaacg aagctcaagc gatattaata atttatttca ttctaagcct gttaaagata
      901 tatctgcatt agacttgaat gcatctttag gtaattttca agagataatt atttatgatg
      961 agcataataa taaattattt gagacatcga atgataacac agtgagagtt gaaccaggtt
     1021 atgaacaccg ttattttgac cgcgtaataa aaaaacgcta taaaggcatt gaatatttaa
     1081 ttattaaaga accaattaca acgcaagatt tcaaagggta tagcttgtta attcattcac
     1141 tagaaaatta tgataacatc gtaaaatcat tgtatatcat tgcgctggca tttggagtga
     1201 ttgcaacaat tataactgcc acaatcagtt atgtattttc aacacaaatt actaaaccgc
     1261 ttgtcagttt atcaaataaa atgattgaga ttcgacgaga tggttttcaa aataaattgc
     1321 aattaaatac aaattatgaa gaaatagata atttagcaaa tacgtttaat gagatgatga
     1381 gccaaattga agaatcattt aatcaacaaa gacaatttgt tgaagatgcg tcacatgaat
     1441 tacgaacacc attacaaatt attcaaggtc atttaaattt gattcagcga tggggaaaaa
     1501 aagacccagc agtattagaa gaatcgttaa atatttctat tgaagaaatg aatcgtatca
     1561 taaaattagt cgaagaatta cttgaattga ctaaaggaga tgtaaatgac atttcttctg
     1621 aagcacagac cgtgcatatt aatgatgaaa ttcgctcgcg aatacactca ttaaaacaat
     1681 tgcatcctga ttatcaattt gatacggatc tgacatctaa aaatctagaa attaaaatga
     1741 aacctcatca attcgaacaa ttatttttaa tctttattga taatgcaatc aaatatgatg
     1801 tgaagaataa gaaaattaaa gttaagacaa ggttaaaaaa taagcaaaaa ataattgaaa
     1861 ttacagatca tggaattggt attccagagg aagatcaaga tttcattttt gatcgctttt
     1921 atcgagtgga taaatctcgt tcaagaagtc aaggcggtaa tggactcgga ttatctattg
     1981 ctcaaaaaat cattcaatta aacggaggat cgattaaaat taaaagtgaa attaacaaag
     2041 gaacaacgtt taaaatcata ttttaatcat gactgagacg tcaatcaaag tcataggatc
     2101 aattttttaa gtacacatta gctgtgacta atgtataaga acaactataa aacaaataaa
     2161 cagtggttct ttatcatttc tgttgtactc ccaaaattta caataaaata catctataaa
     2221 cctagaagaa tcaacgcttt tgttgattct tctttttagc agataaatag gtaaatctac
     2281 tttaacaaat aactaaatag tgatattatt acattgtaag cgtttcaaca tttttgtgga
     2341 gggtgtaaaa tgactaacga aagaaaagaa gtttcagagg ctcctgtaaa cttcggtgcg
     2401 aatttaggtc taatgttaga tctatatgat gactttttac aagatccatc atctgtacca
     2461 gaagatttac aagtcttatt cagcacaatt aagaatgatg actcaattgt accagcttta
     2521 aaaagtacaa gtagtcaaaa tagcgacggc acaattaagc gtgtcatgcg tttaattgat
     2581 aatattcgcc aatacgggca tcttaaagcc gatatttatc ctgtaaatcc tccaaaaagg
     2641 aaacatgtac ctaaattaga gattgaagac tttgatttag atcaacagac tttggaaggt
     2701 atatcagcag gaattgtttc agatcacttt gccgacattt atgataatgc ttatgaagca
     2761 attttaagaa tggaaaaacg ttacaaagga ccaattgcat ttgagtatac acatattaat
     2821 aacaataccg aacgtggttg gttaaaaaga agaattgaaa cgccatataa agtaacgtta
     2881 aataataacg aaaaaagggc actattcaaa caattagcgt atgttgaagg gtttgaaaaa
     2941 tatcttcata aaaacttcgt tggtgcaaag cgtttttcaa ttgaaggggt agacgcactt
     3001 gtaccgatgt tacaacgtac tattacgatt gctgcgaaag aaggtattaa aaatatacaa
     3061 ataggcatgg ctcaccgtgg acgtttaaac gttttaacgc atgtcttaga aaaaccgtac
     3121 gaaatgatga tttcagaatt tatgcataca gatccaatga aattcttacc tgaagatggt
     3181 agcttgcagt taactgctgg atggactggt gatgtgaaat atcaccttgg tggcattaaa
     3241 actactgatt catacggtac aatgcagcgt attgcactgg ctaacaatcc aagtcacttg
     3301 gaaattgttg cacctgttgt tgaggggcgt acgagagcag cacaagatga tacacaacga
     3361 gctggggctc cgacgactga tcatcataaa gcaatgccaa ttattataca tggcgatgct
     3421 gcttatcctg gtcaaggaat taacttcgaa acaatgaact taggaaactt gaaaggctat
     3481 tctacgggtg gttcattgca tattattact aacaatagaa ttggatttac tacagaacca
     3541 attgatgcac gttcaacaac ttattctaca gatgtggcca aaggttatga tgtgccaata
     3601 ttccatgtca atgcagatga cgttgaagct actattgaag caattgatat tgcaatggaa
     3661 tttagaaaag agtttcataa agacgtcgtt attgatttag taggttatcg tcgtttcgga
     3721 cataacgaaa tggatgaacc atcaattact aatccagttc cttatcagaa tattcgcaaa
     3781 catgactctg ttgaatatgt gtttggtaaa aagcttgtta atgaaggtgt catttcagaa
     3841 gatgaaatgc attcatttat agaacaagtc caaaaggaac taagacaagc tcatgataaa
     3901 attaataaag ctgataaaat ggataatcca gatatggaaa agcctgcaga tcttgcatta
     3961 ccgttacaag cagacgaaca atcatttact tttgatcact tgaaagaaat aaatgatgca
     4021 ttgttaacat atccggatgg ctttaacatt ttgaaaaagt taaacaaagt tcttgagaag
     4081 cgtcatgagc cgtttaataa agaagatggt ttagttgatt gggcacaagc agaacaactt
     4141 gcatttgcga caattttaca agatggtaca ccgattcgct taactggtca agatagtgaa
     4201 cgtggtacat tcagtcatag gcatgccgtg ttacatgatg agcaaacagg tgaaacatat
     4261 acacctttac atcatgttcc tgatcaaaaa gcgacatttg atatacacaa ttctccgctt
     4321 tcagaagcag cagtagttgg ttttgaatac ggctataatg tggaaaacaa aaaaagcttc
     4381 aatatttggg aagcacaata tggtgatttt gcaaatatgt cacaaatgat ttttgacaac
     4441 ttcttattca gttctcgctc aaaatgggga gaacgttcag gattaacatt attcttacct
     4501 catgcatatg agggtcaagg gcctgaacat tcatcagcaa gattagagcg atttttacaa
     4561 ttagctgctg aaaataattg cacagttgtc aacttatcta gttcaagtaa ttatttccac
     4621 ttattgcgtg cacaagcggc tagtttagat tctgaacaaa tgcgaccatt ggttgttatg
     4681 tcaccaaaaa gcttactgag aaataaaaca gttgcaaaac caattgatga atttacttct
     4741 ggtggatttg agccaatttt gacagaatca tatcaagcgg ataaggttac aaaagttatt
     4801 ttggcaactg gtaaaatgtt cattgattta aaagaagcat tagctaaaaa tccagacgaa
     4861 tcagtattac tcgttgcgat tgaaagattg tatccattcc cagaggaaga gattgaagca
     4921 ttactagcac aattgccaaa ccttgaagaa gtgtcatggg tacaagaaga acctaaaaat
     4981 caaggtgcat ggttatatgt ctatccatat gttaaagtgc tagttgcaga taaatatgat
     5041 ttaagttatc atggcagaat tcaaagggct gctccagctg aaggcgatgg agaaattcat
     5101 aaacttgttc aaaataaaat tatagaaaat gcattaaaaa ataactaggg ggaaataagt
     5161 catgccagag gttaaagttc cagaattagc agaatctatt acagaaggta ccattgcaga
     5221 atggttgaaa aacgtagggg atagcgtaga aaaaggtgaa gctattcttg aattagaaac
     5281 tgataaagtt aatgtcgaag ttgtatctga agaagcaggt gtattatctg aacaacttgc
     5341 aagtgaaggc gacactgtag aagttggaca agcaattgct atcatcggcg aaggtagtgg
     5401 caatgcttct aaagaaaata gtaacgacaa tactccacaa caaaatgaag aaacaaataa
     5461 taaaaaagaa gaaacaacaa ataattcggt agataaagct gaagtaaatc aagcaaatga
     5521 tgacaatcag caacgtatta atgctacgcc ttctgcgcgt cgatatgctc gtgaaaatgg
     5581 tgtgaatctt gctgaagtaa gtccgaaaac aaatgatgtg gttcgtaaag aagatattga
     5641 taagaaacaa caggcaccgg catcaacaca aacaacacaa caagcatctg caaaagaaga
     5701 gaaaaaatac aatcaatatc ctacaaaacc agtgattcgt gaaaaaatgt cacgtagaaa
     5761 gaaaacagct gccaaaaaat tattagaggt atctaataat acagctatgt taacaacatt
     5821 taacgaagtt gacatgacaa atgttatgga attgcgtaaa cgtaagaaag aacaatttat
     5881 gaaagatcat gatggtacta aattaggatt tatgtcattc tttactaaag cttctgtagc
     5941 agctttgaaa aagtatccag aagttaatgc agaaatcgac ggcgacgaca tgattacgaa
     6001 acaatattat gatattggtg tagctgtttc tacagatgat ggattattag taccatttgt
     6061 aagagattgt gataaaaaga attttgcaga aatcgaagca gaaattgcta atttagcagt
     6121 taaagcacga gagaaaaaac ttggcttaga tgatatggtt aatggttcat ttacgattac
     6181 aaatggcggt atttttggat caatgatgag tacgccaatt atcaatggta atcaagctgc
     6241 aatcttaggc atgcattcaa ttattacaag accaattgcg attgatcaag atacaatcga
     6301 aaatcgtcca atgatgtata ttgcattaag ctatgatcat agaattattg acggtaaaga
     6361 agcagttgga ttcttaaaaa caattaaaga attaattgaa aacccagaag acttattatt
     6421 agaatcttaa tccacaacac aaaatagttt actattccta aaagcgggat taaatcaata
     6481 acaaacagca gtaagattat ttcctagtcg aaatatctta ctgctgtact ttatttttat
     6541 aatgattcgt attaaaatgt gggtttaatt ttgactactt aaaaaatata tcatttctat
     6601 tgaaatagac tcacaataga aatataggta gaatagcgtg tttcaatatg ctaatgaatg
     6661 cattttagat aataacaaac gatgaaatat atgaattcta taagcgcctt tagttaattt
     6721 aaaatcatct gaacatgatg taaattcgct ttatggacac gacatatata tgtttttcat
     6781 gtcctacaaa aaacgaaata ttcaattgct atatacagat attcattaat gacacactca
     6841 atagcaacca taacaatatt atgttaccta tttaaatgaa atagttcttt taaagaaata
     6901 catttttcac atattaatct ataatcaaaa tcaactgacc gatattctat aatttatgat
     6961 taaaataagt tataatataa tagtaaagat aaagatagag gtggctataa tgtgtggact
     7021 tagaagtata acattaggta caacaaatat agaacagaca aaacatttca tggttgacat
     7081 attaggatta aattatgaag aacttcttga aaactcaatt cgtttcggcg atgcagatat
     7141 aagcccagga acaagacttc aatttataca agttccaagt gagcaattag aagaatctca
     7201 ctttgtgggt attggattac gtacaccaac tgactcaggt ttagaggagt atgcggaaat
     7261 attatcgaat aaggatattc catttacaac agttaaagaa ttaaatggca ataaatattt
     7321 cagtctcgaa gataacaatg gtcatatttt ctcaatatat tcaaacgaga ataattatgg
     7381 cgttggttta ggtatgcctt cttttgagag tgcggtcaat ccgttacatc aagtgcaagg
     7441 tttaggacca gtgattctta aagtgaatca tgtagatatt acaggtcaaa ttttaacaaa
     7501 tatattcgga cttgaagtat ttgcagaata ccaacccttc gacaatgctg actatcatgt
     7561 ccaagtattc aaagttggaa cgggtggtct aggtggcgaa atacatttaa tgcctgtaga
     7621 aaccgaaatg acaatgccgg aatatggcgc agtcgatcaa gttgagtttg aaacgaaaga
     7681 tgcagatttc tttaatcaag cgaaatcgcg cttagatgaa gtggaaatac catatcaaac
     7741 gcttgagcaa gatgatattg aatcaattag aattactgaa aacagtggat tatcgtttat
     7801 attcacttta caaaaataat ttttttacga tagcgaggac aaaatttatg ttacatgaaa
     7861 cttggaaaga acgtacacca atcaagaaag tagaagtcat taatacagat gcaaagaaat
     7921 ttacagtttc tgacatgtta acggttggca aacaatatga tgttatcaac gaaactgaag
     7981 aatattatca aataatagat aattctggat tagttggcgg ttactataaa acatatttca
     8041 aagaagttta aacgcataat tattgaaatg aagcctaata tgagattttc aattagtaaa
     8101 tttggtaaaa tataaacaat tcgcatacta agcattgcgt tttatatcag aatagaatag
     8161 taaactaagg catttggaga acttattcca tgtcttagtt ttttaattta tttaaaggag
     8221 aacgttttaa aatggcacta aaacattata agaattcaga ttcaacagtt ttcaatgatg
     8281 cgaaggcatt atttgattta aataaaaata ttttacttaa aggtccaaca ggttcaggga
     8341 aaacaaagtt ggcagaaaca ttaagtgaag ttgttgatac acccatgcat caagtcaatt
     8401 gttctgttga tttagataca gaaagcttat taggctttaa aacaattaaa acaaatgcgg
     8461 aaggtcaaca agaaattgtc tttgtagatg gtccagttat taaagctatg aaagaggggc
     8521 atattttata tattgatgaa ataaatatgg ctaaacctga aacattgcct gtattaaatg
     8581 gggtcttaga ttatcgtcgt caaattacga atccatacac tggtgaagta atcaaagctg
     8641 taccaggatt taacgttata gcagcgataa atgaaggtta tgttggtact ttgccaatga
     8701 atgaagcact aaaaaatcgc tttgttgtta ttcacgttga ttatattgat ggggacattt
     8761 taaaaaatgt gattaaagag caaagtttat tacaaggtga taaacaaatc gaacaaatta
     8821 ttaagtttaa tgaagattta cgtactatgt ctaagcaggg acaaatttct gaagaagccg
     8881 ctagtatccg tgcattatta gacttgtgtg atttaatcac tgtaatgcca gttgaacgtg
     8941 caattaaacg tacaattatt gataaattgg aagatgaacg tgaacaacaa gcaatatata
     9001 atgctgtaga actaaacttt taaagaggga taaaacatga gtgatcgttt cataaaattt
     9061 aatgacgaac agttagatgc caagcaagta atgatgttgc aagacttagc tcgactctta
     9121 ttaaaaaatg aacaaacaca agttaaaatc caaaaattcc cttactataa tcctgttcaa
     9181 aatgttttga ttacgagttg gttttggtca catcgaccaa gccatatcga aatggctggt
     9241 ttaaaaacag atgttatgtt agctgcttac ggttatcata tgatggatgt acaaattgta
     9301 aatgaagtag tacaagataa aacttttaaa caccctaaat tttatcaaca actttttaaa
     9361 ttattagaag atatgcgcgt attaaattct attaaagttg agcgcccaag tactgctaaa
     9421 ttaattgatc ttcgcttaga tacacgtata tcatatacgg aatctcaaat taaagtgtat
     9481 cgtactaaaa cacaatatac agatttatta tttttgtatt tagaacatgc atttttaagc
     9541 caagatttct ttgatatacc atctatacat tcagatttag atgatatttt agtaaatatg
     9601 ttcttatatt tacctaattt tttccaaaat caaaattctg aagataatat gtatctggct
     9661 caaagaatta tgtatcaagt agatgatatt ttaaaagaag atatgctaaa cgaatattat
     9721 tacttaccta aaacactata taatacatta gctagtccag agtttgatga tttaaagaga
     9781 actgatgcaa gtcaagtcga tgggcaagat gatacttcag aagatgatga taacgaaagt
     9841 gaaaaagctg actctaaatc tgcagatagt gagtcaaaag gtggcgccta tttagaaatg
     9901 gagcttcacg aaggacaaaa tagtgaaaca ttaggtaatg atgaagcacg tgaaggcgat
     9961 gctacagatg atatgactga tatgatgacc aaaaagggca aaggatcaaa cgatacgcta
    10021 aatcgtgaag aaggcgatgc tgttggtcaa agccaagcat ttcaacttga tggtgtgaac
    10081 aaaaatgtag aaataaaatg gcaaattcct gaaattgagc cacaatatgt tttagaatat
    10141 caagaatcga aacaagacgt acaatatgaa atcaaagact taattcaaat tataaagaaa
    10201 acgattgaac gagaacaacg agatgcacgt tttaatttaa caaaagggcg attacaaaag
    10261 gatttaatta attggtttat cgacgatcaa tataaattgt tttacaaaaa acaagattta
    10321 agtaaatcat ttgatgcgac atttacattg ttaattgatg cgtcagcaag tatgcatgat
    10381 aaaatggctg aaacgaagaa aggtgtagtg ttattccacg agacgttaaa agcattgaac
    10441 attaaacatg aaattttatc attcagtgag gatgcctttg attcagacga gcatgcacaa
    10501 ccaaatatca ttaatgaaat tattaactat gattactcaa cctttgaaaa agatggccca
    10561 cgtattatgg cacttgaacc tcaagatgat aatcgtgatg gtgttgctat tagagttgca
    10621 agtgaacgat taatgcgtcg aaatcaacat caacgatttt taatcgtatt ttcagacggc
    10681 gaaccgtctg catttaatta tagtcaagat ggtattattg atacgtatga agctgtagaa
    10741 atgtcacgta aatttggtat tgaagtattt aatgtatttt tgagtcaaga tccaataact
    10801 gaagatgttg aacaaacaat tcataatatt tatggtcaat atgctatttt cgttgaaggt
    10861 gttgcgcatt tacctggaca tctctctcca ttattaaaaa aattactact taaatcttta
    10921 taaataaatt taaaatatga gtttatatga aattgtgaat aaaagtcatc agttactatt
    10981 taaaaagtgg atttgaagaa ttttattttc tgacataatt tgatttaaac aacacatgtg
    11041 ttttgtttct gctatgtaca acatatttca tatagactct ttattatttt ttgtgctaaa
    11101 atatttactt gtaaattatt tcgtgtttta aaaaaaggag taactctatg aataaaaata
    11161 catgggtcat tgggtttacg ctcttcgcta tgttttttgg cgcaggcaat ctaatttttc
    11221 ctcctaatct cggattggat agtggtcaat ttttctggcc cgccatttta gcgtttgttc
    11281 taactgggat tggtttacca ttattaggtg tgattgtagg tgcacttgat aaagaaggat
    11341 atattggcgc attaaataaa atttcaccta aattttcaat attgttctta atcatcattt
    11401 atttgactat aggaccactt tttgcaatac ctagaactgc atctacatct tttgaaatga
    11461 caattacacc aattatacat agcaatagta gtatcgcttt atttatattt acgattatct
    11521 acttcatagt cgttttgtat atttgtttaa atccatctaa gttaatcgat cgtattggtt
    11581 cattattaac accattatta ttgattacta ttttagcgat gattattaaa ggatacttag
    11641 actttagcgg taatagtgct ggaaagggca atgaagcact atatcattct aatttttcaa
    11701 gttttgctga aggctttaca caaggctatt taacaatgga tgccattgca gcaattgctt
    11761 tttcaatgat tgttgttaat gcagtaaaac taacaggcat tactaaaaca aatcaaatat
    11821 tcaaacaaac tttgactgct ggtttaattg cagccgtagc tttaattttc atatatattt
    11881 cattaggtta tattggtaat catatgccag taagtgacat gacgttagat caattgaaat
    11941 ccaaagatcg aaacattggg acatatttat taacgacaat ggcttcaaca ggatttggtt
    12001 cattcggaaa atatttattg ggcatcattg tggcgctggc atgtctaact acagcatgcg
    12061 ggcttattgt tgcagtttct gaatatttcc atagaatcgt acctaaagta tcatacaaag
    12121 catttgtatt agttttcatt ttaatgagtt ttattattgc taaccaaggt ttaaatgctg
    12181 ttatctcaat gtcaattccg gtattaagca ttgtataccc agtagcaata actgttgtat
    12241 tattaatttt aattgccaaa ttcataccga caaaacgcat ttcacaacaa attccagtta
    12301 ttatcgtatt tatattgtcg attttcagtg ttattagtaa gttaggttgg ctgaaaatta
    12361 actttataga atcattgcct ctaagagcgt attctttaga gtggttccca gtagcaatta
    12421 ttgcaacgat attaggctat ctagtcggca tatttgtaaa acaagatcca attaaatatc
    12481 aacaggaata acgaataata taaaagaggt tgggacataa atccct
//
LOCUS       DALAAR010000068        12485 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000068 DALAAR010000000
VERSION     DALAAR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.070"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..741
                     /locus_tag="O2458_002114"
     CDS             <1..741
                     /locus_tag="O2458_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887969.1"
                     /translation="ISDDCSMYSKVFEQYKKNVVLDKVYYSEVYKYSDNLIKVFSEDV
                     WNIMGYKKYNEYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQ
                     PENRKTVLIFYKIQPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFK
                     PEYNKQHVNEEFNSLSSSKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLH
                     CVFNKDILNMDKVDIENFFKSIEVKYSK"
     gene            1295..1477
                     /locus_tag="O2458_002115"
     CDS             1295..1477
                     /locus_tag="O2458_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887970.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(1459..1698)
                     /locus_tag="O2458_002116"
     CDS             complement(1459..1698)
                     /locus_tag="O2458_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887971.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1931..3595
                     /gene="alsS"
                     /locus_tag="O2458_002117"
     CDS             1931..3595
                     /gene="alsS"
                     /locus_tag="O2458_002117"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCZ3887972.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            3632..4336
                     /gene="budA"
                     /locus_tag="O2458_002118"
     CDS             3632..4336
                     /gene="budA"
                     /locus_tag="O2458_002118"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ3887973.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4700..5125)
                     /locus_tag="O2458_002119"
     CDS             complement(4700..5125)
                     /locus_tag="O2458_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ3887974.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            5419..6234
                     /locus_tag="O2458_002120"
     CDS             5419..6234
                     /locus_tag="O2458_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCZ3887975.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            6445..7299
                     /locus_tag="O2458_002121"
     CDS             6445..7299
                     /locus_tag="O2458_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ3887976.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(7359..9782)
                     /gene="hysA"
                     /locus_tag="O2458_002122"
     CDS             complement(7359..9782)
                     /gene="hysA"
                     /locus_tag="O2458_002122"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCZ3887977.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVIILNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(9779..9946)
                     /locus_tag="O2458_002123"
     CDS             complement(9779..9946)
                     /locus_tag="O2458_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887978.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            10190..10606
                     /locus_tag="O2458_002124"
     CDS             10190..10606
                     /locus_tag="O2458_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ3887979.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            10676..11524
                     /locus_tag="O2458_002125"
     CDS             10676..11524
                     /locus_tag="O2458_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ3887980.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            11744..11869
                     /locus_tag="O2458_002126"
     CDS             11744..11869
                     /locus_tag="O2458_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887981.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            12131..12485
                     /locus_tag="O2458_002127"
                     /pseudo
     CDS             12131..12485
                     /locus_tag="O2458_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4448 a         1754 c         2096 g         4187 t
ORIGIN      
        1 atatctgatg attgtagtat gtactcaaaa gtatttgaac aatataaaaa aaacgttgta
       61 ttggataaag tatattactc tgaagtttat aaatatagtg ataatttgat aaaagtattc
      121 tctgaagacg tttggaacat aatggggtat aaaaaatata atgaatattt tacaaacaga
      181 gatgatacta cattaaaaat tactaattta tatattgggc aatcttttgg tacagagggt
      241 aatagaaatg tcattgatag aatagggaaa ggtcatgaaa aattacaaga aattatgact
      301 tatcagccag aaaatagaaa gactgtctta atattttaca agatacagcc tgacaatatt
      361 tatattacaa accaatatgg taaagaggct gtgtctcgat taaaaagaat tttagagcaa
      421 agtataccta ataaagaata tattgattta tcagagatat cattaattac attttttaaa
      481 ccagaatata ataaacaaca tgtgaatgag gagtttaatt ctttatcttc ttcgaaaata
      541 aagaatttag ccaaagaaaa tgatggtatt attatttatt taaattttaa ggatgttaat
      601 tatcaactga taagtgaagg caatactttt ttttctgcaa aaccatattt gcattgtgtg
      661 ttcaataaag atattttaaa tatggataaa gttgatatag aaaatttttt caaaagtata
      721 gaagtcaaat acagtaaata agatcaatta ttgaatccac cgaccagcag atttttttaa
      781 aaaattaata ccccgttaat accctttgct tcaatttgat gaaaatcaat gaaattcaaa
      841 attgaagaaa tcctttaata tcaaggtgta cgacagtcta tataatcatg cgaaattcta
      901 aaattttctg aacgtaaaaa aacaaattct taaagcagct cgtcgttcac ctcaattctc
      961 aaaacgttaa ttgtcggacg atatatatac aaaacacctc gatgttatgt cgaggtgttt
     1021 ttttgcgttt gtgtggggaa tatggaatag agtataaatg aattaatgtc tcaggtatag
     1081 aattaattca actatgaatt attgatttac acgtataatt tgtacatgct tacacaatca
     1141 aaagtgtaca ttattaaatt gtcatttagc ttgcggagac cgtacgatag tgttctaata
     1201 atagatattc aagggaaaga gctatcggat gataaccaga tgataactca agaaccatga
     1261 tttaagagtg ttatcaaaat aaggagtgac ttaattgaaa gattctatat tttggaagaa
     1321 agcttttatt cctgtttatt ttattgttgc gatgctggtg tttctacttt ttaggtttta
     1381 tattaaaaca gataactttt ctatatattt aatgagtatc ttcttaattt gtttaggaac
     1441 tgcttctatc atttataact ataaaaccaa tcgataaata taagtatgaa atgtataagt
     1501 cacagttaga tctaagtctt gctgtggctt tttatatctc cataaaacga caaattcaag
     1561 cccgacataa aacagcattt tcagcccacc ataaaacgac aatttcagcc cgccattgac
     1621 taaacaccac atcccaaaaa tatcgtaaca atcctctaca tcaatcaatc caacatccct
     1681 cataatcaca acgcacaaaa tctattcatg catttttgga atacttagta ttacaaataa
     1741 cgatttttat tcatctaaca aaggttatat aatgtactga aggcaatttt tatgtattac
     1801 aaatctaatc gtacatgtaa aattttgata aacatcatta attttgcgta actatcatta
     1861 gattacaaat cacaaagtaa ttacatgtaa tacacatcta tacatcacat ttgaagggaa
     1921 atgaatataa atgactgata aaaagtacac tgcagccgat atggttattg atactttgaa
     1981 aaataatggg gtagaatatg tttttggtat tccgggtgca aagattgact atctatttaa
     2041 tgctttaatt gatgatggtc ctgaacttat tgtcactcgt catgaacaaa acgccgcaat
     2101 gatggcgcaa ggtattggaa gattaacagg caaaccgggt gtagtacttg ttacaagtgg
     2161 tcctggtgta agtaatttaa ctactggatt attaaccgca acatctgaag gggatcctgt
     2221 attagcgtta ggtggccaag tgaaacgtaa tgatttatta cgattaacgc atcaaagtat
     2281 tgataatgct gcgctattaa aatattcatc aaaatatagt gaagaagtac aagatcctga
     2341 atcattatca gaagttatga caaatgcaat tcgaattgct acttcaggaa aaaatggcgc
     2401 aagttttatt agtattccgc aagacgttat ttcttcacca gttgaatcta aagctatatc
     2461 actttgccaa aaaccaaatt taggagtacc gagtgaacaa gatattaatg atgtcattga
     2521 agcgattaaa aatgcatcat ttcctgtttt attagctggt atgagaagtt caagtgcaga
     2581 agaaacaaat gccattcgca aattagttga gcgcacgaat ttaccagttg tagaaacatt
     2641 ccaaggtgca ggtgtaatta gtcgtgaatt agaaaatcat ttcttcggtc gtgtgggctt
     2701 attccgcaat caagttggtg atgaattatt acgtaaaagt gatttagttg ttacaatcgg
     2761 ttatgatcca attgaatacg aagctagtaa ctggaataaa gaattagaaa cacaaattat
     2821 caatattgac gaagttcaag ctgaaattac taattatatg caaccgaaaa aagagttgat
     2881 tggtaatatt gctaaaacga ttgaaatgat ttctgaaaaa gtggatgagc catttataaa
     2941 tcaacaacat ttagacgaat tagaacaatt aagaacacat attgatgaag aaactggtat
     3001 taaagcgacg catgaagaag gaattctaca tccagtggaa attattgaat ctatgcaaaa
     3061 ggtattaact gatgatacta ctgtaacagt tgatgttgga agtcactata tttggatggc
     3121 acgtaatttc agaagttaca atccaagaca tttattattt agcaatggta tgcaaacgct
     3181 tggtgtagca ttaccgtggg caatttcagc tgcacttgtg cgccctaata cgcaagttgt
     3241 gtccgttgct ggcgatggtg gctttttatt ttcatcacaa gatttagaaa cggccgtacg
     3301 taaaaattta aatatcatcc agcttatttg gaatgatgga aaatataaca tggttgaatt
     3361 ccaagaagaa atgaaatata aacgttcgtc aggtgtagac ttcggtcctg tagattttgt
     3421 aaaatatgca gaatcatttg gcgcgaaagg tttacgagtt actaatcaag aagaattaga
     3481 agcggcaatt aaagagggct atgaaacaga tggtccagta ttaattgata tacctgtaaa
     3541 ttacaaagat aatatcaaac tttcaacaaa tatgttacct gacgtattta actaaaataa
     3601 agataaatgt taaagaggag tgggagattt tatgactaat gttttatacc aacatggtac
     3661 attaggcaca ttaatggcag gattattaaa aggaactgca tcaataaatg aattattgca
     3721 acatggtgac ttaggtatcg ctacactaac aggttcaaac ggtgaggtaa tctttttaga
     3781 tggaaaagct taccatgcaa atgaacataa agaatttgta gaattaaaag gtgacgagtt
     3841 aacaccatat gcaactgtaa ctaaatttgt agcagataca agctatgaaa cgaaagataa
     3901 atcttcagaa gcagtttttg cagaaattaa ggaaaagatg ttgagtgaaa atttattttc
     3961 agcagtaaaa atttcaggct tatttaaaaa aatgcatgta cgtatgatgc cggctcaaga
     4021 accaccttat acacgtttaa tcgattcagc tagaagacaa cctgaacaaa ctgaaacgta
     4081 tgtcaaaggt tcagtcgttg gtttctttac accagaatta ttccatggta tcggatcagc
     4141 aggatttcat gtacactttg cgaatgatga tcgtaacttt ggtggacatg tcttagattt
     4201 tgaagtagaa gatgttaaag tagaaatcca aaatatagaa acatttgaac agcattttcc
     4261 aattcaagat aaagatttca ctaaagcaaa tattgactat aaagatattg cagacgaaat
     4321 tagagaagct gaataatgaa tccagaaata taatgacggt ttatgaaaat tgacttcata
     4381 atgcgcgatt tagaaatgat agtttgtaaa tatgattaac catgactaca atagaacaaa
     4441 tatatttata attacgtcta agtaataaaa taaatccctt cactattagc agtagtgagg
     4501 ggatttatta ggttccagat atttgagatt tgctgttatg tttagattat aaattgtggt
     4561 acacactcat ataaaattta ctattgtata ggccaatctg ttactacgag aagcaaacaa
     4621 caataattta caagttcaat aactaaaaag acaaacgcca atttttcagc gcttgcccta
     4681 taaaactatt ttcaaattat tatttaacta caatttgtat ctgtttgatt gtacttggat
     4741 caattgaatt tttagcgtcg tcatttttct ttagattcac tactttttta ttaccatcat
     4801 tcaaagtaag cgtataagtt gctgtttggg cattattaat tttttctgtt gtaacaccac
     4861 gttgagaagc taattcattt tttactttac tgtcaatttc ttgataaaga acatttttat
     4921 tttctgggaa gataaagtaa gttcgatgta atgcagtaat accatctact gaaattgtgt
     4981 aaggaacagt gtgataacca tccacagtca tttctttata gccgttatta ctatctgcag
     5041 atgcttcgtg actcggtaat gcagctccga ccgttgataa taatcccaat gctaaagtgg
     5101 cagtaacaaa tgattttagt ttcatatctc aatctccttt tcgggttgta tgatattaca
     5161 ctatttgtat aacacgtttc ataaagttta aaacatactt tttgcatttt ttatatttta
     5221 atttttagta atagttttta acgagggata tgactcgtgc attttgaaaa gtggtaagca
     5281 atgtttttag tttataggga ttgattagtt gtatataata atcatgaaaa gcatatagtc
     5341 gatcttatac ttttaattga gacttatagt gtatttaagt tatagataaa ttcattaaaa
     5401 tattatgggg gaatgagtat gtcaaagcgt ctattattgt ttgattttga tgaaacatat
     5461 tttaaacata atacaaatga agaagattta agtcatttaa gagaaatgga aaaattgttg
     5521 gaaaaactaa ctaataataa tgaagtgatc actgctgtat tgacaggaag tacatttcaa
     5581 agtgtcatgg ataaaatgga tcaagttaat atgacgttta aaccattaca tatcttttct
     5641 gatttgagtt ctaaaatgtt cacttggaat aatggcgaat atgttgaatc agaaacatat
     5701 aaaaagaaag tcttgagcga accattttta tttgaagata ttgaagatat attacgtcat
     5761 atttctgcgc aatataatgt cgaatttatt ccacaaagag catttgaagg taatgaaaca
     5821 cattataatt tttattttca ttcaacaggt aatcacaaca atgatagtcg tatcctagaa
     5881 gcacttgtca gatacgcaaa tgaccaaaac tatacagcga gatttagtcg aagcaatcca
     5941 ttagctggtg atcctgaaaa tgcgtatgat attgacttca caccgtcaaa tgctggaaaa
     6001 ttatatgcta ctcaatttct gatgaggaaa tataatatac cagtaaaatc aatattaggt
     6061 tttggagata gcggcaatga cgaagcatat ttaagctatc ttgaacatgc atatttgatg
     6121 tcgaatagta gagatgaagc attaaagcag aaatttagat taacaaaata tccatattat
     6181 caagggataa cactgcatgt taaagaattt gttgaaggta agtatgatta ttgattagtt
     6241 gagtgatatg tatacttttt aagtgcaaat attaatattt aagggatgat tagaaaatat
     6301 ggtgaatcga ctacacttta gctttaaaaa attatttata tgttgattta tccaattaag
     6361 tgtagacgat tcaggtagcg caaaaatgac gcaggaactg gagaaagaat tgataccaaa
     6421 attaatataa taggttaagt atatatgaat aaaacaaact ttggggctta tttaaataaa
     6481 agtaagagca ggaaaatata taagattttt gatcaaaatt tgaagcgaga gtttagttat
     6541 tggcagttta ggaaactttt caaatcttat acaaggcagt ctgatgtaca tgaattaaaa
     6601 gtgtgtactc aaaatgatgg tactattgag caaatatgga tagatgaagc aaatcaattt
     6661 ggagtgtcag tgacttggaa agataatagt attatcggat ttgttatgcg tccgttaatt
     6721 aattatgatg cgattaagaa tgaaacgact ttaaaatacg cagtgccgac aacagattgc
     6781 tatgagattt tttggggtgg agataatgaa ttatataatt atcactattt atacccacat
     6841 caaaggtatg ctattgattt agttattaaa gaaaatcata aaacatataa gaatcaagga
     6901 aaagtaaata ctgattattt ttgttttgga aaagatatca ttgcgccagc aaatggtaca
     6961 gttgaaaaag tagtgaatgg ggttcaagac aacagtattg gaagtacgaa tgaatcacag
     7021 tttttaggaa attatattgt aattaagcac gcagaaaatg agtatagctt aatagctcat
     7081 ttacaccaat attcaatcat tgtgaatgag gggcaaaatg ttaaatatgg tgatatcatt
     7141 gggaaggttg ggaattctgg caattctact gaacctcata tacattttca agtaatgaat
     7201 gataagaata ttgaagcatg tacatcttta aaaattcgat ttataaataa tcgagaactt
     7261 atcaaagggg atgtggtctg cggattacaa gctgaatgat ggcgatactt ataaaatctc
     7321 gacactataa aaatggtata gtgtcgagat tttcttgctt atttagttaa ttcaaagtgc
     7381 acgccggatt cattagaagt cgacgtattt ttgtttgtaa tagagtaacc ggtcattgaa
     7441 attttagatt caatatctga agcggaattt gtagattcag gattatagaa gctacattca
     7501 taagtgttat catctttctt tttaagtata aacatacctt tggctttaac ttcgacttta
     7561 gtgttgttaa tgtcaaaagt ttgagtgcta ttactataat taacaccagc ccaaaccgat
     7621 tcattatctt tcacaacgtg gaagtcatct tcttgcttaa cgacagttac ttcatctttt
     7681 tttgtcttaa agacatcttt agataagcct ggatacaaca catatccata tttattgtca
     7741 gaattagaat gcttttgagt tacttcataa tactcatcag ttttttgtgt atccttttga
     7801 cttttattta tttctttcca cttaccagta tgactttctt tttttacagt tattttcggt
     7861 ttgtttaaaa aatgataacc gatgttcttt ttggtatctg tggactctaa aaagactgaa
     7921 ttgttttcct gattatcaga attggttgtt tgtttatcgt ctgtatataa cgtataccca
     7981 ttcgctttgc gattttcaat cgttgtaact ggattctttg atgaatcagt acttttaatg
     8041 ccagttccta agaagacaat tttatcgttt aatatgaaat atgatttttt ggcagttaaa
     8101 gttttgtcct gattttcaaa atccattccg atactagcat gttggtcatc aacttttgtt
     8161 ccgccaacaa aagttttact cgacttttta tcatccgtat cttttaatat ttcattgtct
     8221 aaagttgttg tacctgataa acgtttcata tcggctgtca cccagaagtt atcatgatag
     8281 tgtttgacat cgctgttata taaataagac attccagcac cagtgtgcca accttttaaa
     8341 ttctctccgt tgatactttc atagcgtgct acgtttttcg acgtcatact taaaccaaat
     8401 gcaaagtcta agtctttgtt atgataggtg acacgatcca tgtcattata tattttaagt
     8461 tgttgtgtta atccgttttt agaaatactg ttatctgtca ttaaagactt cattttatct
     8521 atgtccgaat atgaatttaa ataatcattt tgtttataac ttgaatctga ctctactgaa
     8581 gatttgacaa tctttttata cttagctttt gttgaatcat ccatggcatc acttaatctc
     8641 aacaatgatt tcattactgt tgcagatgct gagtgactgg tttcattttc acgactgata
     8701 gctctacctc gtgataaatc catcatttca cctttataaa tgagtggcat aaatccgtcg
     8761 tcaatccatg actttaaggt tgtatcattt tgggttttat cattaaaagg tgtttctttt
     8821 atcatcggca tcatttgaga aatcccctct aagagtacaa cgccataagc accagtgtat
     8881 ggaacgtctt gatgatcaat gtaagagcca tctttataaa atccattacg ttctttacca
     8941 gtggcagaat cttgaacgta agtgaagact ttattaaatg aatctataga ctttttcatc
     9001 atatctttat cttcttcgat aatacattct aaaagtttca ccttagaaat gtctactaga
     9061 tttccgcctt tagcaagttc agcttttcct acagaagata atattttgtc actatctggg
     9121 gcgaaagttt taataggggc agtgaatttt ttcttttctt catttgaaaa ttgatcattc
     9181 agcaatataa gcgtatttgt taatgattta ggtgttccaa tttcataatc ccaccaattt
     9241 aagttggtat tcttgccagt tgttttagta aaattctcac ttaattcttt tactttttta
     9301 tctggttctt ttccatatgc atttttatgc agccactcaa gggcatcttt cactttcttc
     9361 ttattttcgt cagtatttaa agtggtttta ggattcctca tcgcttctgc gattttctca
     9421 atattacgat aggtacgagt catatgagaa gaattagttt caagggtttc cgctcctgac
     9481 cacaagtatt tcctaccact ttcagttttc atttccttga gtaaattcgt cgcctctttc
     9541 tctgtagcat caaacttctt cttcatatct ggattattct catcatactt atcataacca
     9601 tagttaacgt ccagccatgt gttcctcaat ttttcataat ctggcgtttg aacattcgta
     9661 tcagccacag cgatttgatg tttatcaaca cttctgaatt caccaccatt caaaataatc
     9721 acaccagcca ttaataacgt aatggtggat aatttttgcc atttctttat tctatatgtc
     9781 attgacatgt ctcctttttg tgttgcgcgt gcgcaatgaa tattatgatt aaataatgat
     9841 tcaatttttc aaaattcgtt aacgtataca aatgactgtc tactgtcaaa caatccacaa
     9901 agaatgttga tgcatataaa caatcgatca cccaaatttt ccgcatcacc caaaaattgc
     9961 aactaaaata attttcaaaa aattaaatat tatcaatctt agcgattata tcattttaaa
    10021 tatttaagtt atacaatttt taaagtaatt aaaaacgatg aaatgtaaaa tgttaaaaaa
    10081 gagacaaatt taaatttgag taataaaata agaagtattc aaacgaagat gacaatatat
    10141 aaagcaacat tactttagac gaccaaacat aactgaaagg atagtcgcaa tgaaaactaa
    10201 agaagtcgta gcgctcatga atatatctca agacacttta agatattatg aaaaggttgg
    10261 tgtgattcca ccagttaatc gagatgaaaa tggatataga atatataatg atagtgattt
    10321 aaattggatt tatttagtga aaaatttgcg aaatgcaggc gtcagtattg aatcgcttat
    10381 cgaattttgc aggttagcgc agcttcctaa aaatgaaaat attcaagcac agcaaaagca
    10441 aattttaaat aagcaactcg aagaattaaa tgaaaactta aagacaattc atgatgtgag
    10501 agatttacta caatataaaa ttgataatta tgataatcat attgctaaaa tcaatgctag
    10561 tgataattat gatgacaatg ttgaacgtct ttgggagaga aagtaaacct atggcttgct
    10621 atagagttcg ctccatggtt tatagtggac atacaagata aaggaggttc attttatgaa
    10681 tcatattgaa ataagtaaag atgtaaagat accagtatta ggtttcggag tatttcaaat
    10741 tcctcaagaa caaacagcag aagcggttaa agaagcaatt aaagcgggtt atagacatat
    10801 tgatacagca caaagttatt taaatgaaac tgaagtaggt caaggtattg aagcttcagg
    10861 tattgataga agtgaactat tcattacgac taaagtatgg attgaaaatg ttaactatga
    10921 agatactatc aaatcaatcg aacgatcatt acaacgatta aatttggact atcttgatct
    10981 agtgcttatt caccaacctt acaatgacgt atatggctca tggcgtgcat tagaagagtt
    11041 aaaagaaaat ggtaaaatta aagcaatcgg tgtatctaac ttcggcgttg atcgaattgt
    11101 agatttaggt attcacaatc aaattcaacc acaagtgaac caaatagaaa ttaatccatt
    11161 ccatcaacaa gaagaacaag ttgcagcact tcaacaagaa aatgttgtgg tagaagcttg
    11221 ggcaccattt gcagaaggta aaaatcaatt attccaaaat caattgttgc aggccattgc
    11281 agataaatac aacaaatcaa ttgcacaagt tattttacgc tggttagtag aacgtgacat
    11341 tgttgtactc gcaaaatcag tgaatccaga acgtatggct caaaacttag atatatttga
    11401 ttttgaactt acagaagaag ataaacaaca aattgcgaca ttagaagaaa gtaatagcca
    11461 atttttctca catgctgatc cagagatgat taaagcactt acaagcagag aattagatgt
    11521 ttaatcataa tataacaaag taaatcattt acattacact aacctgaaac ttggatgtta
    11581 aagtctatgt ttcaggttat tttttactct taaaaagtga aaggagcaag caaataagtt
    11641 attatatatt tttatattat gtaataacgt aactaaggtt ataattagat aattaatatg
    11701 tcttggaatt aattatttaa taagttgtat agcaggtggc gatatgagat atatacactt
    11761 ttctattatt acattaatta caggtattat tatgcatatt acaatgtact ttgtaccatt
    11821 tgaatctcgg aaaatgccac tatttatgac gataattttt acaatctaaa agcagtaaag
    11881 actcataata taaccaaaaa cattaataaa agtttgttaa atgataaggg gatgagtatt
    11941 ggtgaattta ctcaaaaggt ttaagggaaa aatcctacct ggagagattc aaaaactaaa
    12001 tggactattt ccaaaaataa agggcaatca catggtggtt cttattggaa gttaataaat
    12061 aacaaaggaa aaagaatagc ttctttaact aaagaaggaa aaatcttaag ggaataaggt
    12121 gttgtaaatt atgtgctttg atataaataa tttggatata aaaaagttga attttagaaa
    12181 ggtaaaaaac gcaattcatt taagtttaag tggcgataaa taccagtttt tggaggacgg
    12241 taaaactatc aataatactt atttttagct gtatatgata atgcaataaa catttttgaa
    12301 gatttgtttt cgccttctga ttctataaat ttggttcatg tagtatatgt ttatagtgaa
    12361 ccttataaga aaacaaatgt tataaaaaaa tttacatcgt taacaataga tgaattgtat
    12421 tactttaaag aatataaaaa ggagaaggat gataatattt tgtgcgagga gttttcatat
    12481 aagtg
//
LOCUS       DALAAR010000069        12465 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000069 DALAAR010000000
VERSION     DALAAR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.071"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(67..927)
                     /locus_tag="O2458_002128"
     CDS             complement(67..927)
                     /locus_tag="O2458_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3887982.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            1040..1590
                     /locus_tag="O2458_002129"
                     /pseudo
     CDS             1040..1590
                     /locus_tag="O2458_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1583..2803
                     /locus_tag="O2458_002130"
     CDS             1583..2803
                     /locus_tag="O2458_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3887983.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(2875..3171)
                     /locus_tag="O2458_002131"
     CDS             complement(2875..3171)
                     /locus_tag="O2458_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCZ3887984.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(3220..3423)
                     /locus_tag="O2458_002132"
     CDS             complement(3220..3423)
                     /locus_tag="O2458_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887985.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3732..4718
                     /locus_tag="O2458_002133"
     CDS             3732..4718
                     /locus_tag="O2458_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCZ3887986.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            5017..5118
                     /locus_tag="O2458_002134"
     CDS             5017..5118
                     /locus_tag="O2458_002134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ3887987.1"
                     /translation="MSTRGLNIWQEMEIGNVSHKVAKRAQIPLIIFK"
     gene            complement(5385..6578)
                     /gene="sqr"
                     /locus_tag="O2458_002135"
                     /pseudo
     CDS             complement(5385..6578)
                     /gene="sqr"
                     /locus_tag="O2458_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(6597..7931)
                     /gene="cstB"
                     /locus_tag="O2458_002136"
     CDS             complement(6597..7931)
                     /gene="cstB"
                     /locus_tag="O2458_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCZ3887988.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(7962..9029)
                     /gene="cstA"
                     /locus_tag="O2458_002137"
     CDS             complement(7962..9029)
                     /gene="cstA"
                     /locus_tag="O2458_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCZ3887989.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            9166..9426
                     /gene="cstR"
                     /locus_tag="O2458_002138"
     CDS             9166..9426
                     /gene="cstR"
                     /locus_tag="O2458_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCZ3887990.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            9426..10181
                     /locus_tag="O2458_002139"
     CDS             9426..10181
                     /locus_tag="O2458_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ3887991.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            10279..10380
                     /locus_tag="O2458_002140"
     CDS             10279..10380
                     /locus_tag="O2458_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3887992.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            10396..10848
                     /locus_tag="O2458_002141"
                     /pseudo
     CDS             10396..10848
                     /locus_tag="O2458_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(10942..12465)
                     /locus_tag="O2458_002142"
                     /pseudo
     CDS             complement(10942..12465)
                     /locus_tag="O2458_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT         4087 a         2032 c         1812 g         4534 t
ORIGIN      
        1 ttcattaaca tctcatttaa attattatct ttgagtttca ccataactac acgttttcac
       61 caataattat aaaaaaacat cttttacaaa agattcaagt acttgcttat tcgattcagg
      121 attaattatc aatgttgttt ctaaattcgg aatatcgaca tctacgtcta tttgaaacaa
      181 acttttattt tctaattcat ctgcaacaat aaattccggc aatagcgcaa tcccacctga
      241 atttttcaag tgttttttta atgactcaat tcctagaaat tcaatcattt gttttgttga
      301 aaagctatat ttatataaaa tcatttctaa ctgttctctg taactacacc ctttttcagt
      361 aacaagtaat gtatggtttt gaatatcgtt taatttgaaa tctttcatct ctttgttagt
      421 aacaaatatt aaacgttctt cagttatagg aatagcagtt aagttactat taataatctt
      481 ttcgtctgtg ataaggctaa tatccgattt gaaattagca atttcttctt taatatagaa
      541 attagaatgt gcggatttaa acttcaagtt tacatatggg tgttcagcta accattcact
      601 aatcttatta tagtagcgat acaaatactg actttcttgc atgttaatac ttatatttac
      661 cttcttattg taaagtcctt ttatttctct ctcgtattcg ctattactat ctaaaatcac
      721 tttagcgtat ttaacgtatg ctaagccttc ttccgttagt tccataccat atttttttct
      781 ataaaataat ttaacatcta attgcctttc taatttttca atatgtgtag atacagttga
      841 ctgtgaatag ttaagtaact ctgatgcttt agtaaggttc tttgtttcac tagttactaa
      901 aaatgatttt aaaatgttta agttcaaagt agtacctcct ttagggttcc cttttatcga
      961 atcctgaatt ctaagaattc tgatttaata ataatatatt aaatattatt ataaaggtaa
     1021 atcgaaagga gttatgacta tgattacttg tttatttgga agtagtagaa aggtggcaat
     1081 tcagctatcg cagtagagaa tctattgaag aatttaaaag tcaactttgt gaatttatat
     1141 caaagcaaca ttgaaaaggt tacagataat cgtcatagca aaggtgccaa ctttcataat
     1201 gatgattacg aacaaatttt aatcaaagtt ttagaatcag atattattat tttttcaaca
     1261 ccactctatt ggtactccat gagtgcgtct ttaaaactct tcattgatag atggaccgag
     1321 tcattgcgag atacgcaaat agacaacttc aaagaaatca tgtcacaaaa aaagtatttg
     1381 attttaatca taggtggcga caatccgcat accaaagcac aacctttagt caaccaattc
     1441 aaattaatat ttgagtttat gaatattact aactatagat ttttgatcgg cgaaggcaat
     1501 aaaccattcg atattttaaa cgactctcaa tttatagaag agctcgcaaa tataaatctt
     1561 gcattgaaaa aaggtgatat ttatgactga tttgttgaaa attaaaaact tcagattgtt
     1621 ctttttagca gaaataattt cagcattcgg agttggaatt agtacagttg gggctaattg
     1681 gtatttaatc gacaaaacta acgactccca actattaggt attatgttag ctttaaatgt
     1741 attatccggt ttcttagctt ctcctataat aggtggacta gccgataaat ataatagacg
     1801 aaatattatt ttaatcacct atcttcttca ggtaatatta tatttactca ttgtcatagc
     1861 actcgtaatg ataggatttg agacctattt agtgattgga tttgcgattg ttaatgggat
     1921 aggttggact acgtatatgg caacatctag aagtttagtt aaacagatat taaaaccaga
     1981 tcaatataca gatgcaaatt ctctattaga gattagtttg caaacaggta tgtttattgc
     2041 aggtggatta tcaggaatat tatataaaat aaatggattc actctaatta tagcgatgac
     2101 tataatgatg tttctaatca gcatttttat gttatttaga ttgcatgtag ataaaccaac
     2161 tcattcagag gaagaatcaa caaatagctt attgcaagaa tatttattag gatggaaatt
     2221 tttaaaagac aacatgatga tatttatttt tggagttatt tcaatcatac caatggtgtt
     2281 tacaatgatc tttaacatat cattaccagg atatgtttat aatgttttaa aactttcgtc
     2341 tgtacaattt ggtttttcag atatgttata tggcattgga ggattatgtg caggtttaat
     2401 ttcagctatt ctttcgaaga aaatctcaac taaagtattg atatttttgt tatatttcat
     2461 attagtcata aattcagcac tatttatttg gataaactca gcattttact tattcatagg
     2521 atcatttata ctaggctact caatttcatc aatcagaatt tatatgaata cagctataat
     2581 gaacactgtt tcagataaat atgtcggtcg ctcatttacg atatggacgt caatttcatt
     2641 gttactgcaa agtttaattg ctccattttt aggaagatgg attaatgaaa ttaatgataa
     2701 attcggtttc tatattatac tcattttatc cttactcata tttgttacac tgctgcttgt
     2761 taacaaaaca ggcaaaataa aatatgcaca taaagaagag tgaccgtcac tcttctttaa
     2821 caagcgacca tttatcgatg ggcttagttc tctctgcacc cacacttcac tacttcactt
     2881 tttcaaatca ttttttatgg tcttaaataa atcagtgaga tttgttgctt cggtaaagtc
     2941 tagaattaat atcatttctt tagaacctgg atatggcgaa actaatgtat tatcttgcaa
     3001 tttctgctgg gcacttttag tcgccttgac caataatcta ttatcataca aaccacctat
     3061 aaccacgcca tcataataaa taatatattc tcccatcatc tttcttctct taaccgcgtt
     3121 tgaattcaca tgatttaaaa ataaatcatg tacatctttc ttagtcgcca ttgtaatcgc
     3181 tccttcagtt ttatgtttaa tcacattcgt attaatgatt catttcgtgt tgctcttaat
     3241 tttatctata attatatgcg tagttaaaat caaacctatg gaaaagaaaa taatgataac
     3301 aatgttaaga aatatagtta taaaattata gtttggaagg tatgcgaata gcagaaaaat
     3361 aggtatcgca aaaaataaat cccaccaacc taaacttttt aaagaatgct ttaaaccttc
     3421 cataatatca cctttataaa tttgtctttg ttataagata actaaaaaat cgctttactg
     3481 taaaagtagc caaagaaaat tctgaatcat attcataagt agtgtatcat taataatgaa
     3541 caatttaata ctataatcct tgatctttgt attgatcaac ttaccacaac atttatttta
     3601 gactactctt agacttccct ttcaaatggt tgcatctatt gaaattcctt ttgtataagt
     3661 taggcttttg tggtaatatc atcatgcata aaaaatcgag atactaatta taaagagggt
     3721 ataaatatat tatgaaagaa aatttttgga gtgaattacc acgtccattt tttattttgg
     3781 cgccaatgga agacgttaca gatatcgtct ttcgacacgt tgtaagtgaa gcagctagac
     3841 cggatgtgtt tttcactgaa tttacaaata ctgaaagctt ttgccaccct gaaggcatac
     3901 atagtgtgcg cggacgctta acttttagtg aagatgaaca gccgatggtc gctcatatat
     3961 ggggagataa gccagaacag ttccgtgaaa cgagtattca attagctaaa atgggcttta
     4021 aaggcataga cttaaatatg ggatgtcctg tagcaaatgt tgctaaaaag ggtaagggtt
     4081 ccggcttaat cttaagacct gacgttgctg ccgaaattat tcaagcgact aaagcaggtg
     4141 ggcttccggt aagtgttaaa acacgccttg gctactatga aatcgatgaa tggaaagatt
     4201 ggttgaagca cgtcttcgaa caagacattg ccaatttatc tattcatctt cgtacacgta
     4261 aagaaatgag taaagtagat gcacattggg aattaatcga agctattaaa aatttacgtg
     4321 acgaaattgc accaaataca ttgttaacaa ttaacggtga tattcccgat agaaaaacag
     4381 gacttgaact ggcagaaaaa tatggcattg atggcgtcat gattggtaga ggcattttcc
     4441 acaatccatt cgcttttgaa aaagaaccac gcgaacacac aagcaaggaa ctattagatc
     4501 tattgagatt gcatttatca ttgtttaaca aatatgaaaa agatgaaata cgacaattca
     4561 agagcttgcg tagattcttt aaaatctatg tgcgtggcat aagaggcgct agcgaacttc
     4621 gacatcaatt gatgaacaca caatcaattg cagaagcacg agcactactc gatgaatttg
     4681 aagcccaaat ggacgaagac gttaaaattg aattatagta tgagtaataa agtttatgga
     4741 tgatatttcc caatttaaca cggattgaac actttctact taggtattat cttggttttc
     4801 ctgataggat aactcccgat gatgcctttt tacttatgtc acttatcctt ttggaaatgg
     4861 tgcaaaatta aaaatacatc aggatatcgt ttaactcaga aaaaaactcc aatcaaaaaa
     4921 aatgtttaca acacattatg aaattaaaat tgctcttagt gttcaacaga caaagttatt
     4981 gaaattacta ttggaggtac ataccaagct atcattttga gtacacgtgg tttgaatatc
     5041 tggcaagaaa tggaaattgg taatgtcagt cataaagtcg ctaaacgtgc tcaaatacca
     5101 ttaattattt ttaaataatt tagaggatga ggtataaacc tttaaaaaac agcagtgaga
     5161 tgattttcaa ttagaaaata tcttactgct gtttctattt tcataatcat tcttattgaa
     5221 tgacttcatc gtgatcattt aaaacagtaa caactttttc aatcttatac tatttagagc
     5281 taaaaattca cgacatataa acacaacact gacacgtcat ttaaatactt aatcttttat
     5341 agtaaaagat ttttcattaa aaatttattg ttttctgtac tttattatat taatcctttt
     5401 agcatgccgt accaatacat tttaggtaat aaatctttct taaatatata catacttcta
     5461 cgttctttgg cctgattaaa cggcattgtt tctttagtat ttttattata atcaaactct
     5521 gcaagtatta acctattata tccagtaaca atagggcatg aagtataacc atcataatga
     5581 tgcgttaaca tttgattatt catcacttgc aataaattat tagcgacgat aggtgcttgc
     5641 ttacgaatag ctgcgcctgt ttttgaagta ggtacatttg aagcatcacc aagtgcaaat
     5701 acattagagt agcttttatg ctgtaatgtg gttgggttaa catctaccca accctcacta
     5761 tctgaaagtg tactttcttt tactacatct aagggaccca taggtggtgt tacatgtaac
     5821 atatcataac ttattgtttt tctatcgtat gctttgatat gttcgaatgt agccactttt
     5881 ttgtcaccgt cgatttcaac aaggttataa ttataattga ctgttatatt tctttcttca
     5941 acaatccttt ctaattcttt attatatttt cctacgtcaa ataaagcatc ttttggcgtt
     6001 gcatatatca cattagcgtt agaacggatt ttatgtttcc taaaataatc ttcagctaaa
     6061 tacataattt tcataggcgc acctccacac tttataggag tgtttggatg cgtaaaaatg
     6121 gcatttcctt gtttaaaatt agaaatttgg ttccaagttt cgttaacata gtcaggtgaa
     6181 tagttagagc aaacaccatt tttacctata ttttctttta gtcctttaat tgaagaccaa
     6241 ttaatctgta atcctggagc tactactaaa aaatcataat aaacgactgt attatctcct
     6301 aaaataacgc tattattttc aggttaaaaa cttgaaacag cctgttttat ccagttagca
     6361 ccttcaggta taacactttc catatcttta cgagaacttt tcaaactaga tacccctgca
     6421 ccaaccaacg tccataatgg ttgatagtaa tgatggtctg ctggatctat aattgctatt
     6481 ttctctttta agttttgatt ttttcttaat agtcttgatg ctacggtaac acctgctgta
     6541 ccgccaccaa taataacaat ttgataatgc ttattcattt tttccacatc cttaaattat
     6601 tttaatgatt ctggaaaatc ttgatagcct tctctaatat tcaccacatt ttcaaaacct
     6661 ttgctttcca atatacccac tgcaattgaa cttctaacac ctgactgaca atgtacatat
     6721 attttatcct ctttattaaa aggaatattt tcatttaata atttaccatg cggaatatta
     6781 actgcttgat ctaagtgtcc attattccac tcttcatcat tacgtacgtc taatacatgt
     6841 tcttctttac ctgtcatatc agcgctatga acggactggg ttgaaatgcc tgattttggc
     6901 aaacgatagc ctgctacctt atcaaaccca attaattgta aagtgtgttt cgctttctca
     6961 acagtagatt tatctccaat taaatctata tctttttcaa aatctaagta ccaaccaatt
     7021 tgattaataa agtttttgtt gtatggtata ttgattgttc cttttgtgtg gccaccgtga
     7081 aaggcctctt tgctacgaag atcaaatgct actctcttat tatctaaact aggataaaca
     7141 tcatatgatt gatataaatt cataccaaac tgattaactt gtttcatttg tgcaaaatga
     7201 tgcggtggtg ctggttgatt tgatgttaat gattcaataa atttagtctc atcaatttca
     7261 ttaaatgccc agttattaat tttctcataa cctattgtag atataggtat ggcacctaat
     7321 gctttaccac aagggcttcc agcaccatga cccggccaga tttgaacata gtctggtaaa
     7381 tttttaatat tttgaacgga ctcatacatt tgtttcgcgc taatttctgt agaacccttt
     7441 atttgaacag atttttctaa taaatcaggt ctacctatat caccaacaaa aataaagtca
     7501 ccactaaata atcccatcgg aacacttgaa ccaccaccta aatcagtgag taaaaagcta
     7561 atactttcag gcgtgtgtcc tggagtatgc agaacttcta atttaacatt gcctacttga
     7621 atgatatctc catgtttaac aaattgtgtt tttgatggca tatttttata ccctaatgca
     7681 tcttcacctt cgccagacac atatatattt gcatttaagc gtttagccac atcacgaatt
     7741 cctgaagcaa aatcagcatg aatatgtgtt tctgtagctt gtgtaattgt taaaccttca
     7801 gaatctgcaa cttctatata tttcgataaa tcacgaacag ggtctattat tattgcctct
     7861 cctgtacgtt gacaacccac taaatatgat gcttgagata aatgattatc gtaaaactgt
     7921 ttaaaaaaca tgataacaac tccttttatt aatagattta attagataaa taaattatga
     7981 ttcgcatttt cagtagcacc aatataagtg cctacaccac cgtactcaac ttcatctcta
     8041 agttcttctt tctgaatacc catgacatcc atactcatcg tacaagcgat taatttgata
     8101 ttttgctcaa tagcttgatt gataagtgtt ggtaatgaat caacattttt ctttttcatt
     8161 acgtagcgca tcatcatatt tcctaaacca aacatattca ttttggaaag aggcattcgt
     8221 atattctttt tgggcaacat taaatcaaac atttttgcaa taccttgctt tttaacatta
     8281 actgtttgca ctttttttaa tgcattaagc ccccaaaaag taaagaagat agttacatct
     8341 tttccagcag ctctagcacc atttgcaata atcaacgctg ctacagcttt gtctaattct
     8401 ccactaaata atacaattgt agtaccttta gcagaataat ttatatctaa atcttttgct
     8461 ttttcttttt gaataatcgc attaattcca ttgttatttt catcaagctt aactaaagta
     8521 tgccttgttt gtttcaccca acttttaatg tcactaggga atccagggtc tgtgactttg
     8581 acttcaattt ggtcacctac ttcaatattc ttcatttctt gactaatttt tacaattggc
     8641 cctggacatt gaagaccacg atagttaaat tgtttacgat ctgctttaat ttctatactt
     8701 ttatattctt cttgtgtatc ataactatcg ttttcttctt cataagcttt ataaccacca
     8761 tcaagattta taacgttata accttgttta gctaaatatt tactagcatt agcacttctg
     8821 ttaccacttc tacagattac ataataggtt ttattttttt ctttattgaa tgactcaatc
     8881 tcatcaacag gatgaagtat ggaaccattg atatgtccta atgcatactc ctcttctgtt
     8941 ctaacgtcaa ttaattgccc ttgcttggct agtttttcca attctgcttt actaaattca
     9001 tcgataaact tttctccgta ttgtttcata ataacctccc taaaaatacc tgtaggggta
     9061 ttttaaacag ttggatggaa cttgtcaaat acctctaggg gtatttgaca catttgtgtt
     9121 tatctattaa tatattttta ttcaattaaa agtggaggta tgactatgaa ttatgataaa
     9181 aaaatgatta atcgtattaa tagaatacaa gggcaactaa atggaattat taaaatgatg
     9241 gaggaaggaa aagactgtaa agatgtcatt acacaaataa gtgcatcaaa gagttcactc
     9301 caacgcttga tgggtatcat tattagtgag aatttaatag aatgtgtaaa agcagctgcg
     9361 gatgatgaag aaagctccca agagttaatt aatgaagctg taaacttatt ggtgaaaagt
     9421 aagtaatgga tattgttaat atagttatca tgttactcat tggggtattc ggtgggttca
     9481 tatctggtct agtaggcgta ggtggtgcta tcattattta tccagctatt ttattattac
     9541 caccattatt cggagcccct gcatatagcg catatatcgc atcaggtctt acttctagcc
     9601 aagtcttttt cagtacatta agtggctcat taaaagctcg aaaaaaaaca gagttctcgc
     9661 cacaattagt tctttatatg ggtggcggca tgattatagg gagtatgtta ggtgcatttc
     9721 tagcaaattt atttgatgct acatttgtaa atacggtata tatcattatt gctttacttg
     9781 cattaacatt aatgtttatt aaagtgaaac ctagttccga aaagtcttca tttaataaat
     9841 atttattagt tatcattggc cttttcattg gtattatttc aggtatagta ggtgcaggtg
     9901 gtgccttcat tattattcct atattattgg ttttattcaa attaccaatg aatactgtag
     9961 tcgctaatag tatagtaatt gcttttatat cttcaattgg tgcttttgtc atcaaactta
    10021 ttcaaggtta tatcccttta tatgacgcct tattccttat aatagggagt attatatttg
    10081 ctccaatagg gttaaaatta agtaaaaaag tccctaacat aatacaaaaa tggattatca
    10141 gtattttaat cgtttttgct atagttcaac tcattttata aaactaatcc ttttaactca
    10201 gacagcaatt tcccgaaaaa taatacctct tatttaaaca aatatgtatg cagttcggat
    10261 ttatataaat aaaaattttt gatgaaatat ccatatatgc ttataaatat aaatttatca
    10321 actataacta aaaatgataa aagatttaac gccactgatg accgaattaa tattatttaa
    10381 gtttttatca aacgcatgag taaagtagat gcacattggg aattaatcga agctattaaa
    10441 aatttacgtg acgaaattgc accaaacaca ttgttaacaa ttaacgatga tattcccgat
    10501 agaaaaacag gacttgaact tgcagaaaaa tatggtattg atggcatcat gattggtaga
    10561 ggcattttcc acaatccatt cacttttgaa aaagaaccac gcgaacacac aagcaaagaa
    10621 ctattagatt tgttgagatt gcatctatca ttgtttaaca aatatgagaa agatgaaata
    10681 cgacaattca agagtttgcg tagattcttt aaaatctatg tgcgtggcat aagaggcgct
    10741 agcgaacttc gccatcaatt gatgaacaca caatcaattg cagaagtacg agcactacta
    10801 gatgaatttg aagcccaaat ggacgaagac gttaaaattg aattatagta tgagtaataa
    10861 gggaaaggag gaatttaagt ttcctccttt gtttgtgtga aagtctttta ggtatgaact
    10921 agggtgattt aaaataaaag tttaatttct atcttcagtt ggcgtttcca tataaataat
    10981 tggattaatt ctttgatctt ttaaatagag agaccactct actaatttat ttttaagtaa
    11041 tactgaacct aaattagtta attgaaagtt tttatttatt acgttactag tatgaaccat
    11101 atcttttatg ggcatattat caatagatga tatcggtaaa atccatacta tactaaaatt
    11161 tgtactccgt tgtatatcat taaatatatt ttccgagtta tcaggaatag cttctattaa
    11221 ttcttcaaaa ttatatgatt ctggatattt taaggcgtta attgctatta tattctgttc
    11281 tagactataa agagataaag atgtattaat tcctctttta atcgtgaaaa gctctttgtt
    11341 tttaatattg tataagtctg ccaactctat tccatttcct tttactataa aattcttatt
    11401 tattggtttt atgaattttt tatgtaacat taaatattct tctggcaatc ttcctactat
    11461 ttcttttata tatccttctt ctccttcttt aaatactaaa cctgtgggag ttagtgtgtt
    11521 atacctaatt tctatctcat tcaaatgatc attcaaaagg tcaaaaaatt ctctattaaa
    11581 ataaccccat ttcccatttt gtaataaata tttctttcca tcttccattt caacttctgc
    11641 atgtagtatc gtttttagta gcatattatt tgaatgcccc aaattatcta tcacttcaat
    11701 tctgacatca tttatagaat taacgtcata ttttaataag taatctccta tttcagtgat
    11761 gtaatcaact tcaggatcag tcgcatcaaa tgtctctaat gtattatttt taaaagaatt
    11821 tatatatata tattgacgtc gagcatatca tctaacaaga gtatcatatt acttagttct
    11881 aaaaatcttg atatatctat agatatattt tctgtagttg aagaattcga tagttttttt
    11941 agcaataaag tatctaatac ttctatttta ttcaagtctt ttaaagttac tattctagga
    12001 aattcactaa ttttttgagg aagattaata atagcgttta tttctttgat tatcacacta
    12061 attttatcta tgaattgctg ctttctattc ggtacacgca atgaaatact tgtaccacaa
    12121 gtcattgttt ttccaaaaat ttgaggattc tgtggatgtc cttggactga tatataagat
    12181 tctgaaggtc taacgtaatc tacactattc cttctataat taacaatctc tttaagcctg
    12241 ttttgttgaa aaaaattaac atttttatta actatgtctt catttttgat tgctctttct
    12301 gcaaaatcaa ttccgaagtc ataatcaatc aaattattta tatcatgata tgcttgtcca
    12361 aaaggtataa tatatacatt attttgtaat gttgtatctt ctttgagaaa taatattgca
    12421 tcaaaatgat gaccggattc tgaaaataaa ttgtcatctt cttca
//
LOCUS       DALAAR010000070        12414 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000070 DALAAR010000000
VERSION     DALAAR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.072"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(436..1776)
                     /gene="glnA"
                     /locus_tag="O2458_002143"
     CDS             complement(436..1776)
                     /gene="glnA"
                     /locus_tag="O2458_002143"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCZ3887993.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1795..2163)
                     /locus_tag="O2458_002144"
     CDS             complement(1795..2163)
                     /locus_tag="O2458_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ3887994.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2406..3644)
                     /locus_tag="O2458_002145"
     CDS             complement(2406..3644)
                     /locus_tag="O2458_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ3887995.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(3663..4901)
                     /gene="hflX"
                     /locus_tag="O2458_002146"
     CDS             complement(3663..4901)
                     /gene="hflX"
                     /locus_tag="O2458_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCZ3887996.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5013..5489
                     /locus_tag="O2458_002147"
     CDS             5013..5489
                     /locus_tag="O2458_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ3887997.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(5711..5944)
                     /gene="hfq"
                     /locus_tag="O2458_002148"
     CDS             complement(5711..5944)
                     /gene="hfq"
                     /locus_tag="O2458_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCZ3887998.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(5959..6894)
                     /gene="miaA"
                     /locus_tag="O2458_002149"
     CDS             complement(5959..6894)
                     /gene="miaA"
                     /locus_tag="O2458_002149"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCZ3887999.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(6912..7826)
                     /locus_tag="O2458_002150"
     CDS             complement(6912..7826)
                     /locus_tag="O2458_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3888000.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(7976..9649)
                     /locus_tag="O2458_002151"
     CDS             complement(7976..9649)
                     /locus_tag="O2458_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCZ3888001.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(9807..11303)
                     /gene="glpK"
                     /locus_tag="O2458_002152"
     CDS             complement(9807..11303)
                     /gene="glpK"
                     /locus_tag="O2458_002152"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCZ3888002.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11432..12250)
                     /locus_tag="O2458_002153"
     CDS             complement(11432..12250)
                     /locus_tag="O2458_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCZ3888003.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         3821 a         2416 c         1740 g         4437 t
ORIGIN      
        1 atgaaaatac cgatcgataa acaaaaagat attttttgtt ttgtttctct tttcatatag
       61 tattaccccc ttaataatgc gtagtaaggt ccctcttttc ggggtcttac cttagaacgt
      121 tctgcaaatg aattcgatga gaagtaatat gaatatggct attttcaagt aatactcaac
      181 gttttcgcga cgttctttta tcgcctcatc tcatcacctc caaatatatt aaaattcatg
      241 tgaactaaaa tataaaatgg tcttccccag ctttaaaaaa ataaatacat aaaacattta
      301 cttgaccaaa acttgaccaa atccattttt taacaaaagt ggtcaagtga aatcccatct
      361 ttccaaatca aaaaagagct aaagcaaaat gctctagccc ttgatattac tgatttccca
      421 tcattgttta gcgtattaat attgcttcat gtactgatct ctttcccatt cagagacttg
      481 agttctgtag taatcccatt caattgattt tgaattaata aattgattat agatatgatt
      541 tcctaaagct tttttaataa cttcattttc acgcattgct tttaatgcag tataaagtgt
      601 tgaaggtaag tcttgaatgc ctactgcttc acgttcttca cggttcattt cgtaaatatt
      661 ttggttaact ggttctggaa cttttaattt atttttaata ccatctagtc cagcttctaa
      721 gattgcagct aacgccatgt atgggtttgc agctggatct actgaacgta cttcgatacg
      781 agtagataat cctcttgaag atggtacacg gattaatggt gaacggtttt taccactcca
      841 tgcaatataa catggtgctt cataaccagg tactaaacgt ttatatgagt ttactaacgg
      901 gttacataca gcagtaaatc cgcgtgcatt tttaagcaca cctgctgtaa attgatatgc
      961 agtttccgtt aagcccattt cagtatttgg atcaaagaat gcattttctt tacctttgaa
     1021 taatgaaacg ttaaagtgca taccgctacc attcacaccg aataatggtt taggcataaa
     1081 tgttgcgtgt aaattatgtt tacgtgcgat tgttttaaca accaatttaa atgtttggat
     1141 attatcacat gctgtaacag catctgcata tttaaagtca atttcatgtt gaccaggggc
     1201 aacttcatgg tgactagctt caatatcgaa gcccatatcc tctaattcta aaacaatatc
     1261 acgacgacag ttttcaccta aatctgtagg tgctaaatcg aaatatccac catcatcatt
     1321 aagttctaaa gttggttccc ctttttcatc caacttaaac aagaagaatt ctggttcagg
     1381 ccctaggtta aagtctgtga agcctaaatc ttccatttct tttaatacac gttttaagtt
     1441 tgcacgagga tccccttcaa atggtgttcc atctgtttta tatacatcac aaattaaacg
     1501 tgcaacttta ccttgtccag cagtccatgg gaagattacc caagtatcta aatctggatg
     1561 taagtacata tctgattctt cgatacgtac gaaaccttcg atagaagaac cgtcaaacat
     1621 catttcgtta tcaagtactt tttctaattg gcttacaggc acttcaacat ttttaattgt
     1681 tcctaaaata tcagtgaatt gtaatcttaa atatcttaca ttttcctctt ctgcaaattt
     1741 acgaatgtcg tctttagtga aagtacgttt tggcatttta aaatcctcca aattttattt
     1801 aataaatcgg gataaatcac cacgatttat tggcaaagtt tctccaatag gcttttgcgt
     1861 ggcatctaca atcatctttt ttcttatctc ttgttcatct gttgttaaat gctcttgtga
     1921 gtcataaatg atttgtttaa tccctttgat attaaatcct ttttctaata atgatttaat
     1981 ttctagtaat ctttccaaat cattgagtga gaacagacgt ttttgacctt ctgttctttc
     2041 aggtttgatg agttcatgtg tttcatagta acgtatttgc cttggcgtta aatccgttaa
     2101 cttacttact acactcatag agaagacagc catatttcgt ctgattgcat cattcgatat
     2161 cattgttcct ctcctctact tttgaacttg ttaataactt atcataattt ttttaggatt
     2221 tcaaaaatat gtcaggtttt ctaacataaa gcttaaaaca ttgatataac tgcgatttcg
     2281 acttctaaaa cgacataaaa caatctacag ctaaaatatt ttttcaattt ttaaaaataa
     2341 tctgataaat ctccgcaaaa aatttcaccc aaaacacatt atcatttagg gtgaaattta
     2401 atctattata taagtccttg ttctttcaac tgattaacag ctcttgtcac agcaatttta
     2461 acgtgttcat atgttaatcc tccttgaaca tatgcttcat aaggaggacg aataggtcca
     2521 tctgcagata attcaatcga tgaaccttga ataaacgtac cagctgccat aataacatca
     2581 tcttcgtaac caggcatata actaggttct ggactaaaat gtgcattaat tggggatgcg
     2641 tgttgaatac tttgacaaaa tgaaatcatt tgttctttcg tttcaaattt aactgtttga
     2701 attaaatcag ttcgtttttc gtagtacttc ggcgttgtgt tcatattcat tttttctaaa
     2761 aataaactag taaacaatgc acctttaaga ctctgactga caacgtgtgg tgctaaaaag
     2821 aaaccttgat acatttcaag caatgcattt aatgatgcac ccgcttcttt accaatacca
     2881 ggtgctgtca atctataacc acatcgttca attaaatctt ttctaccagc aatgtatcca
     2941 ccaatcttag ctaaaccacc gccagggttt ttaattaatg atcctgctat taaatcggca
     3001 ccacattcta taggttcacg tctttcaaca aattccccat aacagttatc cacaaatatt
     3061 aaaatattag gatgcacgtt tttcaaccta gtaattacct tttcaatttc atctagcgga
     3121 attgaaggtc tttgatcata gcctttcgaa cgttgaatcg ctattacttt ggtgcgctca
     3181 gaaaccccat ctaacacact ttcaatatcg atcttacctt ctttaagtgc aatatcttta
     3241 tacgatacgc catgctccat taaactttca ataccatttc cgtttacgcc aatgacttca
     3301 agtaaagtgt catatggact acccgttata taaattaatt catcaccatg ttttaataaa
     3361 ctttgtaatg caatagtaat cgcatgcgta cctgaaataa tttgcggacg aacaattgca
     3421 tcttctgctt taaatgcctg cgcatatatt tcttctaaat gatcacgtcc aaagtcatca
     3481 tacccgtatc ctgttgatcc ttgtagatca ctttcggttg ctttgacatg atgaaatgca
     3541 tttaatactt tttcttgatt aatatatgct gtttcttcaa tttctttaaa atatggtgct
     3601 aacgttgatt cgacgtcagc tactatctta cttatatctt tcatttgaat ttcgtccttt
     3661 tattattttt taaatccttt tatacgataa acattttcaa tttcatcata tttaagttca
     3721 gtcactaatg tatgttgttt tagaaaatat aatcgatctg cattattcgt cgcaattgtc
     3781 tcatcataat aagttaattg ccttttgatt tcatcaataa ataacgtctt aacaagtaat
     3841 ttatcaccat catttttaga agaaacaaaa acattaggca aatcacttgc tggacgattt
     3901 gatgcatgat cacataagtc ctttttatta aaaataacta tttgagaaat atgactcata
     3961 tctaattgtt tgattaaatc attaactgtg tcatactgcg tacggtattc aggatggcta
     4021 ctatctacga catgtactaa taaatctgca cctttagcct cttctaaagt tgatttaaaa
     4081 gctgcaatta acgtcgtagg tagtttctgt ataaaaccaa cagtatctga aataattaaa
     4141 ttaaatccat catttatttg aatttgtcgt gttttaggat ctaacgttgc aaataattga
     4201 tctttttcat acgtctcttc atttgctaaa acattaaacc atgatgattt accagcattt
     4261 gtataaccaa ctaaagctac ttgaaacacc tgattttgat ttcttttatt tcgatatctt
     4321 tcgcgatgtt cttctaccgt ccgcaattga tgtttaattt cattcatacg agttcgaata
     4381 tgtctgcgat ccatctctaa cttcgtttca ccagggcctc tagttccaat accgccacct
     4441 aaacgagaaa ggcttttacc atggccttgc aatctaggta ataaataatc aagttgtgct
     4501 agctctactt gcaatttacc ttctttactt cttgctctta atgcaaatat ttcaagaatc
     4561 aactgagttc tatcaataat ttttacacct aaagcttcat ttagtgattt ggattgtgca
     4621 gtcgttaatt catcatttgt gatgactaca tcaatatctt tgaactcaat aaatgcttga
     4681 atttcttcaa ttttaccttt accaacataa tatttgcgat ctacacgatc tctgttttga
     4741 gtaatttgac ccaacacttc aagttggcaa gtctctgata aagatgataa ttcctccatt
     4801 gtagactcaa aattaaattg cttatcatct tgagcatgta caccgactaa gacagctttt
     4861 tctagtttat ttttagtatc atgaatttgt tgctgagcca tgttatacat ctccttaata
     4921 aaatcctact caaagtattc tatcatagag ttttagtaaa gactattaca cgattttgtg
     4981 gtacatttta agtaaatcga aaggatgata caatggagac aatttatgat tttgtagtag
     5041 aaacaaataa aggtgttact tacaaattag atgcatataa gggtgacgtt atgctaattg
     5101 ttaatacagc tagcgaatgt ggctttactt cacaatttga aggtttgcaa tcattatacg
     5161 agaaatacaa agatcaaggg tttgtaattc taggtttccc ttgtaatcaa tttggtggcc
     5221 aagaacctgg ttcaggcgaa gaagcagctc aaaactgtaa attaaactat ggtgttacct
     5281 tccctatgca tcaaaaaata gacgtaaaag gggaacatca attaccttta tttagatatt
     5341 tgactgcggc acaacacgga ttctttaatg agaaaatcaa atggaatttc actaagtttt
     5401 tagtggatcg cgaaggtaac gttgttaaac gatttgcacc tcaaaaaaaa cctgttcaaa
     5461 tcgaaagaga aatagaaaaa ttattataaa atatactagc tcatagcaaa taaaacgctt
     5521 catataaagt cgtatcatat attgactcga cctttatatg aagcgttttt attacaaaca
     5581 ttcctctatt gtatcttgtc atttgaacat catttaattg atgtcacata tgactttatt
     5641 tctatatcta tttatcatac ttcaaacgtt atgtacacga gtcattaaat cgtgcccata
     5701 tgattacaac ttattcttca ctttcagtag atgcttgacc ttcagtttct actgtataag
     5761 tgctgatcgc atgtttgtaa atcaagtgtt gtttgccttg agaatttaag cttacgacat
     5821 acttgtcgta ttcttcaata acacctttca tttggaaacc gtttagaaag aatacagtta
     5881 cttcagtttg gtttgcttta aaattctcta gtgctttgtc ttggatgttt tcgtttgcaa
     5941 tcatctgtcg gactcctttt acttaatctg ggttgtaatc tcatctaaca tcatttgaag
     6001 tgacatattt tctttatcta accaatgaac actcatttta ttcttgaacc atgtcatttg
     6061 tcgttttgca tattggcgtg aatgttgctt taaatcattg acagcatctt cataaatcat
     6121 ttgtccgtta atcacaggta ttaattcttt atatccaata gcttgcatac tttggcaaga
     6181 ttcatagcct tgttcaacaa gttgttgcac ttctctaaat aatccgtgat ccaacataat
     6241 atcaacacgt ttatttattc ttgaatataa tgttttacgc gacatttcaa tccctaataa
     6301 taatgtatca taattttcag taaattgttg cactttcttg cgattactca aaagtttttt
     6361 tgtttttaaa taatattcaa tagcgcgcaa cactctttgg cggttgttag ggtgaatatt
     6421 ttctgcagaa accgcatcaa attgagctaa ataatcgtgt agttgctgat tatctaaatg
     6481 ttctaatgca gataactttt gtttaactat ggataattgt gcaggtgtaa ctgtttcatc
     6541 ttctaattca taattatata ttaatgattg aatatataag cctgttccac ctgctatgat
     6601 tggaacttta cctctattcg ttatatcagt aattaaatct tctgctaatc gcttgaattc
     6661 atatgctgaa aatgtatcat caggattcaa gatatcaatt aaatgatgtg gaataccatc
     6721 catttcttca ggtgttactt ttgcagttcc aatattcata tgtttgtaga cttgcataga
     6781 gtcaccgctt atgatttcac cattgatacg cttcgcgagt tcgatgctaa gctctgtttt
     6841 acctgaagca gttggcccca caattactac aataaaaggc ttatttttat tcatatttaa
     6901 ttccactttc tttatttttt ataaaattga ttcaaccatt taaacaagtc ttcccaaatt
     6961 ttgtcatgat ctttttcaaa taacacttca tgacgtttat gatgataaag attcacttta
     7021 acatttttaa tgccagcttt tctatatata ttcgccaatt ttaaaatccc tttaccataa
     7081 tcacctaaag gatcgtcata tcctgaaact aataatatag gcatggcatg atttaacatt
     7141 ttcatatttt ttaattgtga tgtatgtagc atataatagg ctgtttgata taataattga
     7201 tttgacacat taaatccact atatggatct ttaataaagt tatctacttc aattggatta
     7261 ctagaaatcc aatcactttg tgtacgtaat ggacgtattt ttttattaaa actatttgat
     7321 actaactggt taacccattc aactcgtttc tcagcaccat aaatttttgt aatcagttgt
     7381 aacactttaa cggttggtaa acctttccat aatgaataca taccagtacc acttaaaatt
     7441 agaccatcaa catattgcgg atatgtttct acaaacaatc tagctataac ggatcccatt
     7501 gaatgtccga ttataatgta tggtttatca acattgcctc tcactgtttg cgctacttca
     7561 aaggcatcac cgataactcg tttcatatca tcgtaatgcc ctcttgttga ttcatcaata
     7621 ttaataccat gtcctcgatg attatgacgt atcacatcga agccatgctt tgaaagtgca
     7681 tgtgctaatt tatcgtaacg ttccatatgt tcagccatac catgaaatat atgaataatt
     7741 ccttttgaat ctacatctgt aaaattcact ttaacttcaa tattcgtatt atcttgtaca
     7801 gttaatttaa attgtttttc agtcatcatc tcacaccttt aataacaacg cttttatatt
     7861 cgcttttatt attatcatac atttagtgat gcatagtttc aattgactaa tgtatctcgt
     7921 ataaaaaaac tgatgccctt aaaacaggac accagtttat gattgtacaa attaattatt
     7981 ctttaactgc tggttgatta tttccatgtt gcgcttcttt aattgcttgc tcaacttctt
     8041 cagtatatgc ttcaatttga gctggagagt aatcaagcat atctgccata atatcgatga
     8101 cagcatcttt ataatctaat acatctttaa tattgaaata cattttacca gaacgacgaa
     8161 ctaagaaatc gttaggtttg tataccattt cttgttgaat actataaaca agttctacat
     8221 aaatttctaa tggtaacttg ctatcatggt attgagatgt ttgcgcaatg ttgaacaatt
     8281 catcaacatt tgaaccatat ttagatgcta aacgtcttgc aacatcttca tcaatgccga
     8341 atcctttagc tacatctact ttttgctcta caaacgcatc aaagttcttg ctaccaccta
     8401 catcgccacc tgaaattgcc agaccttttg tattacatgg actaaatgtt aaaccgtagt
     8461 cttttttcaa gcgtttagat actaaatcaa caatgtcttg agccatgtga cgatagcctg
     8521 ttaatttacc acctgcaata gttaataaac ctgatttacc ttcccaaatt tcatccttac
     8581 gagagatttc agaagggtct ttgccttctt cgtaaattaa tggtctaatt cctgcccatg
     8641 ttgattcaat atcttcatct gtaacattaa cactagggaa catgtaatta atcgcatcga
     8701 ttaaatagtc tctgtcttct tgtgtagtta atggtgaaga tttgatattg tcatagaatg
     8761 tatctgtagt acctacatac gcttttcctt cacgtggaat tgcaaaaatc attcttccat
     8821 ctttttcagt atcaaagtat actgcttgac ctaatgggaa ttttgattga tcaataacaa
     8881 catgtacacc tttagttaaa cgtaattttt tattattgcg tgcataatca ccacttctaa
     8941 catcatcaac ccatggacct gctgcattaa ccactttttt agccttaatt gtataatttt
     9001 cattagttaa tttatctata actttaacac catttacttg ttgattttta tcataagtga
     9061 agtgttcaga tttagtatag ttgataattt ctgcgccttt ttcagcagca cgcttcataa
     9121 cttcaatagt taaacgcgca tcgtcagtac gatattcaac atagtaaccg ccgcctttta
     9181 gaccttcttt tttaactaat ggttctttag ctaaagtttc ttttttagat aacatttttt
     9241 tacgttcaga cttcttaaca cctgctaaac gatcatacat tcctaaacca attgatgttg
     9301 agaatttacc aaatgttcca cctttatgca ttggtaaaag catccactct ggagtcgtaa
     9361 catgaggccc attttcataa acaatcgcac gttctttacc agtttcggca actactccaa
     9421 tttggaattg ttttaagtaa cgcaaaccac catggactaa ttttgtagat cttgagcttg
     9481 ttccttgtgc aaagtcttgc atttcaacta atgcaacttt cattcctctt tcactcgcgt
     9541 ctagtgcaat acctgcacct gtaataccgc caccaataat tactaaatca tattcatcat
     9601 ttcttaaatt ctttttaata tgttctctct taaaagtaga caatgccatt ccaaaacgcc
     9661 tcctaaaaaa tgtaaaaacg agagacttgt cccacataaa caatccttca attgtacatg
     9721 ttcgaacaaa tctctcgctt ctcgattcaa atattaactt aaattgattg tatcacaatg
     9781 tttatacaaa agtctaatct acaagtttat tctgttttaa aaacttgtgt tgcttcaaca
     9841 gcttttttcc aacctctata taatttttct ctttcgcctt catccatttt cggatcgaat
     9901 ttttcttcta atttccagtt tttagcgata tcatctttac tctcccagaa tccaactgct
     9961 aaacctgcta aaaatgcagc acctaaagct gtagtttctt gaatttcagg tctttcaaca
    10021 gaagtattaa caatgtctgc ttggaactgc ataataaagt tatttttaac tgcaccacca
    10081 tcgacacgta aactttggac atcaatacca gagtcttttg acattgcttc cataacgtca
    10141 cgagtttggt aacatagtga ttctaaagtt gcacggataa agtgctcttt ttcagttcca
    10201 cgtgttaaac cgaaaatcgc accacgtgct tcagaatccc aatatggtgt tcctaaacct
    10261 acaaaagctg gaacaacata aacaccctca gtagagtcaa ctcgtgtcgc ataactttct
    10321 gattgtggtg ctgaattaat cattcttaat ccatcacgta accattggat tgctgaaccc
    10381 gaaacaaaga tggaaccttc aagcgcataa tttacttttc catcaatacc ataagcaatt
    10441 gttgttaata aaccactttc agatttaacc gctttgtcac ctgtattcat taacatgaag
    10501 ccaccagttc catatgtgtt tttcacgtca ccacgttcga agcaagcttg tccaaataat
    10561 gctgcttgtt gatcaccagc tactccagcg attggtactt cttgaccata gaagtggtaa
    10621 tcaattgtct taccatatac ttcactcgaa gctttaactt ctggcaacat atttttaggt
    10681 actgtaagta gttctaataa ctcatcgtcc cattctaaat catggatatt aaacattaat
    10741 gtacgactcg catttgaata atcagtaata tgcgcagctt ttcctgataa tttccatact
    10801 aaccaagtat caatcgttcc aaataataga tcgccatttt ctgctttttc tcgtgcacct
    10861 tcaacattgt ctagaatcca tttaactttt gtacctgcaa aatacggatc taaaagtaat
    10921 cctgtcttat ctctaaatgt ttgttcatat ccttgttgtt ttaattctga acaaattgat
    10981 tgtgtttgac gtgattgcca aacaattgcg tgataaattg ggcggccagt atgtttgtcc
    11041 caaacaaccg ttgtttcacg ttggtttgta ataccgatac ctgcaatttg atcagctcta
    11101 acatcatttt cattaattac ttccgtcatt acagctaaca cagatgtcca aatttcattt
    11161 gcatcatgtt caacccaacc tgattgtgga aaatattgct taaactcacg ttgtgctacc
    11221 cctgcaattt cccctttttg attgaataaa atcgctcttg agcttgttgt tccttggtct
    11281 atagataaaa tatatttttc cattagtagt atctccttta ttaaaaaata atcgctaacc
    11341 cattgtacaa aatctgttaa attttggcga aaaaatagca taatccatga atcaaacatg
    11401 ttaaattatg ctatttacgt ttctttttat tttagtaaat tgattcgata tcaccttttt
    11461 ttgatgattt atttaaaatg taacctaaaa tcaaagtaat aataactaca acaattgcaa
    11521 ttgcacaacc aatattaaat gtatgtttat aaaatacagc gtataccact gcacctaata
    11581 aaccaccggc aattggtcct aagataggaa cgattgcata tgaccaattt gaaccacctt
    11641 taccagctat tggtaaaatc gcatgtgcaa ttctcggacc taaatcacgt gctgggttga
    11701 ttgcataacc agtagcaccg cctaaactta atccgattgc aacaattaat gctccgacaa
    11761 ttaaaggatt taaaccatcg gcaattttgt ttacaccgat aaataaaata cctaaagtta
    11821 atgccattgt tccgataatc tcacttaaaa agttggcaaa gtaattctta atagccggtg
    11881 ctgtagagaa aacacctaat ttcgcgccag cttcttctgt cgctttccaa tgtggcaagt
    11941 acattaacca tacaattgtt gctccgacaa ttgcacctaa catttgagca acaatataac
    12001 caggaactaa tgaccaatca aaacttccgt ctaatgcaag agctaaagac accgctgggt
    12061 ttaaatgtgc acctgagaat tgaccgacag caaacacacc cattgtaacc gctaatcccc
    12121 atccagctgt gatgacaatc caatcagcac cattcgcagc acttctcttt aaattgacat
    12181 tggcacaaac gccaccacca aaaaggatta agattgcagt tcctaggaat tctgctaaat
    12241 atacattcat attaaatgtc ctcccaaaaa gacaaagaga ttcctacact agcagtttgg
    12301 ctcgtagaaa tctcccgtct ctattctatg tattaacttt tattatattt gttatgatac
    12361 tatcgatatg aaagcgttgt caatggtttt tgtaaaaatt ttgtcaaatt taat
//
LOCUS       DALAAR010000071        12153 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000071 DALAAR010000000
VERSION     DALAAR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.073"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(116..1018)
                     /gene="cvfB"
                     /locus_tag="O2458_002154"
     CDS             complement(116..1018)
                     /gene="cvfB"
                     /locus_tag="O2458_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCZ3888004.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1165..2766
                     /locus_tag="O2458_002155"
     CDS             1165..2766
                     /locus_tag="O2458_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3888005.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3626..4831
                     /locus_tag="O2458_002156"
     CDS             3626..4831
                     /locus_tag="O2458_002156"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ3888006.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4895..5884
                     /locus_tag="O2458_002157"
     CDS             4895..5884
                     /locus_tag="O2458_002157"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCZ3888007.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5886..6773
                     /locus_tag="O2458_002158"
     CDS             5886..6773
                     /locus_tag="O2458_002158"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCZ3888008.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6770..7492
                     /locus_tag="O2458_002159"
     CDS             6770..7492
                     /locus_tag="O2458_002159"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCZ3888009.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7519..8238
                     /gene="dapD"
                     /locus_tag="O2458_002160"
     CDS             7519..8238
                     /gene="dapD"
                     /locus_tag="O2458_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCZ3888010.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8381..9532
                     /locus_tag="O2458_002161"
     CDS             8381..9532
                     /locus_tag="O2458_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ3888011.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9537..10622
                     /locus_tag="O2458_002162"
     CDS             9537..10622
                     /locus_tag="O2458_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ3888012.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10612..11877
                     /gene="lysA"
                     /locus_tag="O2458_002163"
     CDS             10612..11877
                     /gene="lysA"
                     /locus_tag="O2458_002163"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCZ3888013.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
BASE COUNT         4226 a         1911 c         2231 g         3785 t
ORIGIN      
        1 ttcgtttgaa ttatttaatt ttttactttg aaaggaatgt ttaaaaaact acttcatctg
       61 tccgatttaa tattgaataa aaaaaacacg catcctacga cacgtgtaaa aatgattatt
      121 cttttgagtc cattcgactc caaccttttt tagttaaagc gattttacct gtttctatat
      181 taataatctt ctgtttatat aagtgaccga ttgcacgttt gaatgaacct ttactcatat
      241 tgaatacttc tttaatcgct tcagggcttg atttgtccca gaatggtaat tcaccatcat
      301 attcaactag taaatcaaag atgacttggc cgtcatcgtc taaacgttca tgtgcaagtg
      361 gtaaaaatga accatttaac tcacctttat cattatgccc gataattcta acttgaacag
      421 attcacctaa tcttggttca gctttacgtt ctgattcatg tacgaaaatt ttgtaacctg
      481 attcgcttaa taaaaagcta ccaattcgta atacgcggta aggtttggct tcaatgactt
      541 cgttttttaa attatcgtcg tgtacaggtg taaacatatt ttctacaaca gattcactcg
      601 ctaaacgtcc atacatatga ttctcacggt caattcgtaa tgtaactagc aaataatcac
      661 caggttgtgg ccatagtgat ttcacttttg gtaaatcttc ccatggtact aacacttcac
      721 ggggtaatcc aacatctata cgtgccccat cgcgatccgt tttaagtact ttagcaaagt
      781 catatttatc tttcgtaata tcaggcatat tttgagttgc aaataattca cctgaacggt
      841 ttggataaat gaagaaacta tattcttcac ctacttctaa ttcatcatca tcgttcattt
      901 ctgattggtt taactttacg ttttcaccgt ttggtccttt taaaaggtaa gttgaacctt
      961 gtaaccctac tacttcaagg aattctatag aacctactat atctttgtct aatgccatgt
     1021 tattctcctt cgattcttgt attctttatt attataacat gtgtgagtca ttctatcatt
     1081 acaacttata tgaggtcgaa actgtgacga taatatgata taattcttcc gttagaatat
     1141 cgaatgaaaa aaaggagaaa atgcatgtta caagtaactg atgtgagttt acgttttgga
     1201 gatcgtaaac tatttgaaga tgtaaatatt aaatttacag aaggtaattg ttatggatta
     1261 attggtgcga atggtgcagg taaatcaaca ttcttaaaaa tattatctgg tgaattagat
     1321 tctcaaacag gacatgtttc attagggaaa aatgaacgtc tagctgtttt aaaacaggac
     1381 cactatgctt atgaagatga acgcgtgctt gatgttgtaa ttaaaggtca cgaacgtctt
     1441 tatgaggtta tgaaagaaaa agatgaaatc tatatgaagc cagatttcag tgatgaagat
     1501 ggtatccgtg ctgctgaact tgaaggtgaa tttgcagaaa tgaatggttg gaatgctgaa
     1561 gctgatgctg ctaacctttt atctggttta ggtatcgatc caactttaca cgataaaaaa
     1621 atggctgaat tagaaaacaa ccaaaaaatt aaagtattat tagcgcaaag tttattcggt
     1681 gaaccagacg tactattact ggatgagcct actaacggtc tcgatattcc agcaatcagt
     1741 tggttagaag atttcttaat taactttgat aatactgtta tcgtagtatc gcatgaccgt
     1801 catttcttaa ataatgtatg tactcatatc gctgatttag acttcggtaa aattaaagtt
     1861 tatgttggta actatgattt ttggtatcaa tctagtcagt tagctcaaaa gatggctcaa
     1921 gaacaaaaca agaaaaaaga agaaaaaatg aaagagttac aggactttat tgctcgtttc
     1981 tcagctaacg cttctaaatc taaacaagca acaagtcgta aaaaacaact cgagaaaatt
     2041 gaattagatg atattcaacc atcatcaaga agatatcctt tcgttaaatt cacacctgag
     2101 cgcgaaatcg gtaatgactt actaatcgtt caaaatctat ctaaaacgat tgacggtgaa
     2161 aaagtattag ataatatttc attcacaatg aatccaaatg ataaagcaat tttaattggg
     2221 gatagtgaaa ttgcgaaaac cacattgctt aaaatattag ccggtgaaat ggaaccagac
     2281 gaaggttcat ataaatgggg tgtaacaacg tcattaagtt acttccctaa agataactca
     2341 gaattctttg agggcgttaa tatgaacctt gtagattggt taagacaata cgcaccagaa
     2401 gatgagcaaa ctgaaacatt tttacgcggc ttcttaggcc gtatgctatt tagtggagaa
     2461 gaagttaaga aaaaagctag tgtactttca ggtggagaaa aagtacgttg tatgctaagt
     2521 aaaatgatgt tatcaagtgc aaacgttctt ttacttgatg aaccaacaaa ccacttagac
     2581 ttagaaagta ttactgctgt taatgatgga cttaaatcat tcaaaggttc tattatcttt
     2641 acttcatatg actttgaatt tatcaataca atcgcaaacc gtgttatcga tttaaataaa
     2701 caaggtggcg tttcaaaaga aattccatat gaagaatatt tacaagaaat cggcgtttta
     2761 aaataaacat aatatgtaga atttcttttc gaaaatctct gtattgaggc ccgaccaact
     2821 tgcacattat tgtaagttgg cttactgtca ccttctatat tgggacttat tccgcaactt
     2881 gtattgtttg tagaaattag gaatccaatt tctctatgtg ttgcaccgac aaggattgaa
     2941 aaaagctagc tacaaagtca ttttcattca gtcaactact gtcaatataa cattgcaggg
     3001 cctagggcat tgattcatgt ccgagactat attcaattta atagacatta actgggcgtc
     3061 tcgatgctaa tatttatagc atcgaggtgt cctttttatt ttatttttta acacatcagc
     3121 atagtgaaca ccccttctct aaattacaaa tacagttatc aacgcattaa aagagtacat
     3181 ttatttaatg tgcatattga ttatgctata agtattttga attttatttt tcaaaaagtc
     3241 tcattatcac caccttttaa aatataagca acgctataac ttgaattttc aggttattcc
     3301 aaaaaatcta aattatcggt ttacattcat atttgtcata tgtataatga acacatacca
     3361 atttaataat attttgatga ggcgcatcaa tcatgagtaa agtttagatt actgtctgct
     3421 aacagctaaa tttgaaaggg tgcgatgccg aagcaattat aatagcagtt ataatttgtt
     3481 ggactttttg gttaagagct gagagtttgt cattatttaa aaataatgga gtgcatcact
     3541 tgtatataga ttaagagcaa gttcgcattc cgaacttgtt ctttttttat tattgtgtgc
     3601 ccttcctaac aattaggagg attatatggt aacaagaagt gtgttgaaat ttggcggatc
     3661 atccgtcagt gattttacaa aaataaaaag gatcgctgaa atgttaaagg agcgagtcaa
     3721 tcaagatgaa caattaattg tcgttgtaag tgctatgggt aacacaacag atcaattaat
     3781 gacgaatgta tcaaccttga ctaaagcacc aaaacaacaa gaactggcat tattattgac
     3841 aaccggagag caacaaactg tatcttattt atcaatggta ttaaatgata tcggtatgaa
     3901 tgccaaagca atgactggct atcaagcggg tattaaaacc attggccatc atttaaaaag
     3961 taaaattgct caaattaatc ctcaaacatt tgaacaagcc tttcaagaaa acgatatttt
     4021 agtagttgct ggatttcaag gcatcaatga acatcaggaa ttaacaactt taggcagagg
     4081 tggttctgat acgaccgctg tggcacttgc tgttagtaat caaatacctt gtgaaattta
     4141 taccgacgtt gatggtgtgt atgccactga cccaagactt ttaccaaagg ctaaacgact
     4201 agacatcgtc tcatatgaag aaatgatgga aatgagcgct ttaggtgctg gtgtacttga
     4261 aacaagaagt gttgaattag ctaaaaacta taatatccct ttatatttag gaaaaacttt
     4321 atcgaacgtg aaaggaacat ggattatatc aaatgaagaa atattagaga aaaaagcagt
     4381 tactggtgtg gctttggata aacatatgat gcatgtaaca attagttatc ccctacctga
     4441 caatcagcta cttacccaac tatttacgga acttgaagaa ggtgctgtaa atgttgatat
     4501 gatttcacaa atcgtcaact tggatgggct acaactatcc ttcacgatta aagatagtga
     4561 ttttcatcaa atttctatga ttcttgaaac attaaagaat caatatgaag cattagctta
     4621 taaaatcaat gagcattatg tcaaaatttc attaattggc tcaggcatgc gtgatatgtc
     4681 aggtgtggca tcaaaagcat ttttgacatt aattgaaaat aatatacctt tctaccaaac
     4741 aacaacatct gaaataagta tttcatacgt cattgatgat tttaatgggc aacaagcggt
     4801 agaaaaacta tatgacgcat ttaacattta atggtaaaat gattgttaaa atattctaaa
     4861 aattggaaat tattataaaa tggagtgaca agttatgaca aagttagcag ttgtgggtgc
     4921 aacaggatta gtaggtacaa aaatgttgga gacattaaat cgtaaaaata ttcctttcga
     4981 tgaattagta ttattttcat cagcacgttc tgcagggcaa gaagttgaat ttcaaggaaa
     5041 aacatataca gttcaagaat taactgatgc tcgtgcaagt gaacatttcg attatgtatt
     5101 aatgagtgct ggtggcggta caagcgaaca ctttgcccca ctttttgaaa aagctggtgc
     5161 aatcgttata gacaattcaa gtcaatggcg tatggcagaa gatattgatt taatcgttcc
     5221 ggaagtcaat gaacctacat ttacaagagg tatcattgcc aatccaaact gctctacgat
     5281 tcaatctgtt gtacctctaa aagtattgca agatgcttat ggtttaaaac gagtggcata
     5341 tacaacatat caagctgtat caggttcagg gatgaaaggt aagaaagatt tagctgaagg
     5401 tgtaaatggt aaagcaccag aagcatatcc acatccaatt tataataatg tgttaccgca
     5461 tattgatgtg tttttagaaa acggatatac aaaagaagaa caaaaaatga ttgatgagac
     5521 gagaaaaatt ttaaatgcgc cagacttaaa agtaacagca acatgcgcac gtgtgcctgt
     5581 tcaagatagt catagtgttg aaattgatgt aacgcttgac aaagaaacaa cagcagaaga
     5641 tattaaagcg ttatttgatc aagatgaccg cgttgtttta gtagacaatc cagagaacaa
     5701 tgaatatcca atggcaatca attctactaa taaagatgaa gtgtttgttg gccgtatacg
     5761 tagagatgat tcattagaaa atactttcca tgtatggtgt acatcagaca atttattaaa
     5821 aggtgctgca ttaaatgctg tacaagtatt ggaacaagtt atgcgtttaa aaggagcgaa
     5881 ttaaaatgac acatttattt gagggtgttg gcgttgcact tacaacccct tttacaaata
     5941 acaaagttaa tattgaagct ttgaaaacac acgttaattt tttactagaa aataatgccc
     6001 aagcaatcat cgttaatgga actactgctg agagccctac tttaacaaca gatgaaaaag
     6061 aacgcattct aaaaacagtt attgatcttg tagataaacg tgttcctgtc atagcaggaa
     6121 ctggcactaa tgatactgaa aagtcaatcc aagcttcaat ccaagctaaa gccttagggg
     6181 ctgatgcaat tatgttaatt acgccctact acaacaaaac gaaccaacgt ggtttagtca
     6241 aacactttga agcgattgca gatgctgtga aattaccagt cgtgctgtac aatgttcctt
     6301 caagaacgaa catgacaatt gaaccagaaa ctgtagaaat attaagtcaa catccttata
     6361 tagttgcttt aaaagatgct acgaatgatt ttgagtattt agaagaagtg aaaaagcgca
     6421 ttgatacaaa ttcatttgca ttatatagtg gcaatgatga caacgtcgtc gaatactatc
     6481 aacgtggcgg tcaaggggtt atctctgtta ttgccaatgt cattcctaaa gaatttcaag
     6541 cgttatacga tgctcaacaa agtggattag atattcaaga tcaatttaaa ccaatcggca
     6601 cactgttatc agctttatca gttgatatta acccaattcc tattaaagct ctaacaagtt
     6661 atttaggatt tggaaattat gaattacgtc taccattggt tagcctagaa gatacagata
     6721 ctaaagtgct tcgtgaaaca tatgacacat ttaaagcggg tgaaaatgag tgaaaatatt
     6781 actaattggc tatggcgcaa tgaatcaacg cgttgctaga ttagcagaag aaaaaggaca
     6841 tgaaatcgtt ggggtcattg aaaatacacc gaaagcaaca acgccatatc aacaatatca
     6901 acatattgca gatgttaaag gtgccgatgt tgcaatagat ttttcaaatc caaatctgct
     6961 tttcccttta ttagatgaag attttcattt gccattagtt gtggcaacaa ctggcgagaa
     7021 agaaaaacta cttaataagt tagatgaatt gagtcaaaat atgcctgtgt ttttcagcgc
     7081 gaacatgagt tatggcgttc atgcattgac taaaatttta gcagctgctg ttcccctact
     7141 tgatgatttc gacatcgaat tgactgaggc acatcataat aaaaaagtag atgcaccaag
     7201 tggtacgtta gaaaaattgt atgatgtgat cgtatctttg aaagaaaatg taacacctgt
     7261 gtatgataga catgaattaa atgaaaaacg ccagccacaa gatattggta tacattctat
     7321 tcgtggaggt acgattgtcg gtgaacatga agttctattt gctggcactg atgaaacgat
     7381 tcaaatcacg catcgtgcac aatcaaaaga tatttttgcg aatggtgcaa tacaagcagc
     7441 agaacgctta gttaataaac caaacggctt ttatacgttt gacaacttat aaacatatta
     7501 aaggagatcg attattttat ggtacaacat ttaacagctg aagaaattat tcaatatata
     7561 agtgatgcta aaaagtctac accaataaaa gtatatttaa atggtaattt tgaaggcatc
     7621 acatatccag aaagttttaa agtatttggt tcagaacaat ctaaagtaat cttttgtgaa
     7681 gcggatgatt ggaaaccttt ttacgaagca tatggtagtc aattcgaaga tatagaaatt
     7741 gaaatggatc gtcgcaattc tgctattcca ttaaaagact taacaaatac gaatgcacga
     7801 attgaaccag gtgcgtttat tagagaacaa gccattattg aagatggtgc tgtcgttatg
     7861 atgggcgcaa caattaatat tggcgcagtc gttggcgaag gtacaatgat tgatatgaat
     7921 gctactctcg gtggtcgtgc tacaactggt aaaaatgtac atgtaggggc tggcgcagta
     7981 ttagcaggtg tgattgaacc ccctagtgct tcaccggtta taatcgagga tgatgtatta
     8041 atcggtgcaa atgcagttat tttagaaggt gtacgtgttg gtaaaggtgc tattgttgca
     8101 gctggcgcga ttgtgacaca agatgtacca gctggtgcag ttgttgctgg tacacctgca
     8161 aaagtgatta agcaagcttc tgaagtacaa gatactaaaa aagagattgt agcagcatta
     8221 agaaaactga atgactagtg acgtcaacgt ataataattt caaggttgag atacgattat
     8281 gtctcaacct tattgtttag cattgaataa acttatgatc acaaatttcg aaatacgtta
     8341 cactatttat atagtaattt aacacaaagg attgataatt atgaatgaat tagaatttgt
     8401 tacgaaacat cgccgtcatt tacatcaaca tcctgaatta agcttacatg aatttgaaac
     8461 aactgcttat attaaagcgt ttttagatag tttaaatatt aaatacgatt gcccattgga
     8521 aactggcgtc attgcatact tagaaggtaa tggctcacat acgatagcgt atagagctga
     8581 tattgatgcg ttacctattt tagaggaaaa tgatgtgcct tatcgcagtc aatctgatca
     8641 tgtgatgcat gcttgtggac atgatggtca tacaactgca ttaatgcttt ttgtacaacg
     8701 ttgcaaagac atgcaagatg caggtcaatt accgcaaaat gtcgttttca ttttccaacc
     8761 tgcagaagaa actggtggcg gtgcaaatcg attaataaaa gccggtgcct ttgataagta
     8821 tccaattgaa gcggtatttg gtattcatgt taacccattt gctgatgaag gcattgcagt
     8881 gataagagat gaagaaatta cggccagcgc aacagagtat cgctttttct taacaggcct
     8941 gtcaagtcat gttgctgata aagaacaagg tcattcttgt ggtgaagcat tacaacatgt
     9001 attaactcaa atatcacaaa ttcaacaatt tcaccttaac ggtttgaaac gaaatattgt
     9061 tcatattggt cattttaaag ctggtgaagc gattaacact gtaccaagta atggctattt
     9121 agaaggtact attcgtacat atgatattga tgatttaaca atcgttaaaa atcaaatgca
     9181 caagatagca gaaagtgtca agcttctgtt taatgtagat tgtgaagtta aatttgcaga
     9241 aggttatccc cctacaatca atagtccgaa attacgtact caaatagagg acgccttaat
     9301 aaaagctgat ttaaatgtct atgacaaacc aacgccattc ttatttgggg aagattttag
     9361 tttttatggt caacaactag ctccagctta ctttgttttt ataggaacac gaaatgaaga
     9421 taaaggtttt gtaactggtt tgcacacatc acatttaaat tttgatgaaa aagtgttaat
     9481 aaacgtggtt aatttttacg aaaatttatt aaataattac aaagaggtgt aatacattga
     9541 cagcaacatg gtctgtaaat aagaaaatat ttttacaaaa tgcaatcaca gtcaaaaaca
     9601 atcagccatt aatggcagtt gttaaaaata atgcatatca ctatgaccta gaatttgctg
     9661 taactcagtt tatccatgca ggtatagata catttagcac aacatcacta cgagaagcaa
     9721 ttcaaattag acaacttgct ccagatgcaa caatcttttt aatgaatgca gtttacgagt
     9781 ttgatttagt ccgtgaacat caaatacata tgactttgcc gtcgttgaca tattactata
     9841 accataaaaa tgatttagct ggtattcatg ttcacttaga atttgaaaat ttattacatc
     9901 ggtctggatt taaagattta aacgaaatta aagaagtatt gaaagatcac catcataatc
     9961 aaaatgcaaa aatgattatt agtggtttat ggacccattt tggatatgct gatgaattcg
    10021 atgtgtcaga ttataatgtt gaacgttcac aatggatgga aattgttgaa gcacttttat
    10081 ctgaaggtta tcagttcgac ctaatccatg ctcaaaatag tgcgagtttt tatcgggaag
    10141 gacaaatatt actaccccac catacacatg cgcgtgtagg tattgcgtta tacggttcaa
    10201 gaccatatag ttcactgaat caacatgata tagttcagtc attaacttta aaagcacatg
    10261 ttattcaagt gcgcgaagta caagctggtg attattgcgg ttatagcttt gcctttgaag
    10321 tgactaaaaa caatacaaaa ttagctgtag ttgatatcgg ttatggcgat ggaattttaa
    10381 gaactcgtgc taaacatgaa gcacttatca atggtaaacg ctacccgata cgtgcattaa
    10441 tgatgagcca tatgtttgtt gaagtagatg gcaatgtaca tgcacaagat gaagttattc
    10501 tttataataa tgatatccgc atcgatgaat atacctttaa aggtgttggt gcaaattctg
    10561 aacaattaag tgctatgaat catgattctt taaaaaagga gtacatttca aatgactgtt
    10621 aaatataatc aaaatggcga attaacaatg gatggtatta gtttaaaaac gattgcacaa
    10681 agctttggta cacctaccat tgtttatgat gaactacaaa ttagagaaca gatgcgccgt
    10741 taccatcgcg catttaaaga tagtggatta aaatacaata tttcatacgc ctcaaaggca
    10801 tttacttgca ttcaaatggt caaacttgta gctgaggaag atttacagtt agatgttgtt
    10861 tctgaaggtg aattatatac agctttagaa gcaggttttg aaccgagtcg catccatttc
    10921 catggtaaca ataaaacgaa acatgaaatt aggtatgctt tagaaaataa tatcggttat
    10981 tttgttatag attcattaga agaaattgaa ttaatagacc gctatgctaa tgatacggtt
    11041 caagttgtat tacgagttaa tccaggtgtt gaagcacata cacacgaatt tattcaaact
    11101 gggcaagaag atagtaagtt tggattatca attcaatatg gcttagctaa aaaagcaatt
    11161 gacaaagtcc aacaatctaa acacttaaaa ttaaaaggtg tacattgtca tattggttca
    11221 cagattgaag gtacagaagc atttattgaa actgctaaaa ttgttttacg ttggcttaaa
    11281 gagcaaggca ttcaagttga attattaaac cttggtggtg gctttggtat taaatatgtt
    11341 gaaggtgacg aaagtttccc tatcgaaagt ggtattaaag atattacaga cgcaataaaa
    11401 tccgaaatta aagttctagg tatagatgca ccagaaatag gtattgaacc gggacgatca
    11461 attgtaggtg aagctggcgt tactttatat gaagttggaa ccattaaaga aattccagag
    11521 attaataaat atgtttcaat cgatggcggt atgagtgatc atatcagaac tgcactttat
    11581 gacgcaaagt atcaagcatt gcttgttaat agaaatgaag aagcagatga cagtgtaact
    11641 atagctggaa aattatgtga gtctggtgat atcattatta aagacgctaa attaccttca
    11701 tcagtcaaac gtggagacta tcttgctata ttatcaactg gtgcatatca ttactctatg
    11761 gcatccaatt acaatcaaat gcaaaagcct tctgtgtttt tcttaaaaga tggcaaagca
    11821 cgtgaagtta taaagcgaca atcgttaaga caactcatta ttaatgatac aaaataaaaa
    11881 taaacaaagt aatccccgaa gcacagaaaa ttatactgtg actcggggaa tttttatgca
    11941 ttatagatga ctgtagaaaa catgcttgga catatactct aaaaaaggaa gaacagcaca
    12001 tttttagtta gaaagtattt atttcttttt gtcattaatc ctttatattt agttagccct
    12061 attcaactaa tttaaagcat catatacata ccacacttta aacaagcatc atgaaattag
    12121 attttatgct ttacttgctt tttcaaataa tat
//
LOCUS       DALAAR010000072        12049 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000072 DALAAR010000000
VERSION     DALAAR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.074"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            33..161
                     /locus_tag="O2458_002164"
     CDS             33..161
                     /locus_tag="O2458_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888014.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(305..2233)
                     /gene="abc-f"
                     /locus_tag="O2458_002165"
     CDS             complement(305..2233)
                     /gene="abc-f"
                     /locus_tag="O2458_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCZ3888015.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2486..3121
                     /locus_tag="O2458_002166"
     CDS             2486..3121
                     /locus_tag="O2458_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCZ3888016.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3425..4504
                     /locus_tag="O2458_002167"
     CDS             3425..4504
                     /locus_tag="O2458_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCZ3888017.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            4564..4788
                     /locus_tag="O2458_002168"
     CDS             4564..4788
                     /locus_tag="O2458_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCZ3888018.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            4995..6245
                     /locus_tag="O2458_002169"
     CDS             4995..6245
                     /locus_tag="O2458_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCZ3888019.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6428..7378
                     /locus_tag="O2458_002170"
     CDS             6428..7378
                     /locus_tag="O2458_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ3888020.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7527..9011
                     /locus_tag="O2458_002171"
     CDS             7527..9011
                     /locus_tag="O2458_002171"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCZ3888021.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            9008..9967
                     /locus_tag="O2458_002172"
     CDS             9008..9967
                     /locus_tag="O2458_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCZ3888022.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10155..10262)
                     /locus_tag="O2458_002173"
     CDS             complement(10155..10262)
                     /locus_tag="O2458_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888023.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(10334..11050)
                     /locus_tag="O2458_002174"
     CDS             complement(10334..11050)
                     /locus_tag="O2458_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3888024.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11069..>12049)
                     /locus_tag="O2458_002175"
     CDS             complement(11069..>12049)
                     /locus_tag="O2458_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCZ3888025.1"
                     /translation="KISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNK
                     GFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLK
                     EMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYF
                     NKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSI
                     NLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHE
                     LFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         4082 a         1701 c         2135 g         4131 t
ORIGIN      
        1 atttcataaa gaaaatgtct taacaatgaa ttttgataaa aacaatgtca atagagaaca
       61 tgcgtactta tcaacaaaga gtgaataact tgtggataaa aggtgttgat aacgtcattt
      121 attgtggata tcaggtggga aaacagaaaa taaatgattg attagtgtta atttacttgt
      181 gggtaactag gataagtctg tggatagcta taatatcagc gtttttattg tgaataaaaa
      241 aattaaaatt tgtaaaaaaa taactctaac tatagcgatt ttacagttag agttatgtat
      301 atttttatag tttttgttgt aattcttccc atttagacat agcgtgttct aatttttgtt
      361 cgctatcttg tttttggata gctaattcgt ttgctttttg tggattgtta tacacttctg
      421 gttgggttaa ttgctcgtct atttgtaaga tagtagtctc taaagtttct atttcatttt
      481 cacactcgga tatttgtcgt tcaagtttgc gttgttcacg tctttgttct ttttggttat
      541 gatacgacga ttgttccata gactttttat gtgcttgtac gttaactgac tgttcttctt
      601 ggtgcgcttt caacgcttcc gcttcctcaa ctttttcaat gtaatattga taatctccta
      661 aatacatctt tccgccatca attgttaaat caaatacctt gtttgctaat tggttaataa
      721 agtaacgatc atgggaaaca aatagtattg ttccttcaaa atgttggagt gcttgctcta
      781 acatttcttt tgaatctata tcgaggtggt tggtaggttc atctaaaata agtacattgt
      841 cacgttgcaa cataagtagt gctagtagta aacgtgcttt ttcaccacca gataaatcat
      901 taattatctt tttaacatcg tcttgtacaa ataagaaacg tccaagaact gctcgaatat
      961 ctttttcatt cattaacgga tattgatccc acacataatc taaaatcgtt ttactagatt
     1021 taaattctgc ttgcttttga tcataataac caatttgtaa atttgcgccg aaagtaatat
     1081 cgccattaag cgctttttgt tgattagcaa tagttttaat taaggtcgat tttccaatac
     1141 catttggccc aatgattgct atatgatcgc ctttagagac ctctatactc ataggtttgg
     1201 taattgcagt ttgataaccg atttctaaat tttttacatg catgacgtca ttacctgtat
     1261 tccggtcaaa gccaaattga atatttgcac ttttggcatc taacattggt ttatcaatgc
     1321 gttccatttt ttctaaaatc ttacgtctac tttttgccat tccacttgtt gaagcacggg
     1381 taatattttt ctcaacaaaa gtttctaatc gttttatttc tgcttgttga ctttcatatt
     1441 cttgcattcg tttttgataa tataaatccc gttgctgtat aaattcctcg taattaccaa
     1501 catagcgttt gacatcacct aaagccacat cataaatttg agtaactatt ttatctaaaa
     1561 agtaacgatc atggctgatg atgacgattg cacctttaaa ataacgtaga taatcttcaa
     1621 gccactttgt cgtttctaaa tctaagtggt tagtaggttc atctaaaagt aataaatcag
     1681 gttcgtttaa tagcatttga gctaaagata aacgtgtttt ttggccaccg ctaaaatcat
     1741 tgataggttt attgaaatct tcttcactaa aatttaaccc gtaaagtact gttttaattt
     1801 tactttcata ttgatatcct tctaattgtt caaattgatt cgataaagat tcataacggg
     1861 acatatgtgt tttatatgta tcactatcgt aatcatttgc atgttttgat aaacaatctg
     1921 tttcttcttt gattaagctt tccattcgtt taatatgttc aaatggtttt gacatttctt
     1981 caaaaactgt tgcattagaa tttaaagtca tttgttgagt taaataacct agttttaagt
     2041 ttttaatttt tgacacattt ccagaatcat agttttctac gccagctata attttcatca
     2101 atgttgattt accggcgcca tttcttccta cgataccaat tcgttcacca gtttttactt
     2161 caaaatcaac atcagtaaat atatcttcac catcgaacga ttttgatata tgattgagtt
     2221 gtaaaagtat cattcgatct tcacctttcg tctttctcaa attttattaa cttatgaaaa
     2281 aagtcgtaat tattacatta ataaccattt tacagtataa aacgccgtct tgaaactaag
     2341 atatttattt ttaaaattca ataatgtata atatttaggt attcattgta atcaattatt
     2401 tgttttttaa taatctaaca attacgctct aactcgtttg aatacataga tataattcat
     2461 tcaaacacga aggaggaaat agcgaatgag tgaccaagtt aaaattcctc gagcaacttt
     2521 aaaacgtttg ccgttatatt atagatttgt cagttcatta aaatctaaag gtatagatcg
     2581 tgtaaattca aaagcgatta gcgatgcgtt acaaattgac tcggcaacaa ttcgtcgtga
     2641 cttttcatat tttggcgaat taggtaaaaa agggtacgga tataatatag atagtttatt
     2701 ggatttcttt aaatctgaac taagcgagag tgacatgatc aaaatcgcaa ttgtcggagt
     2761 tgggaaccta gggaaagctt tgctcacata taacttttca atacatgacg atatgacgat
     2821 tacagaagcg tttgacgtaa aagaagatgt tattggccag aaaataggga acgttattgt
     2881 taaagataac gatgaattaa taacaacatt gaagaaggaa gaaatagatg ttgtgattct
     2941 aactacacca gaaagagttg cacagaaagt tgcagatgaa ctcgtccaag ctggtgtgaa
     3001 aggtatttta aacttcactc ctggtagaat taatacgcct tcagatgtgc aagtacatca
     3061 aattgactta ggtatagaat tacagtcatt attattcttt atgaaaaatt acagtgaata
     3121 aatgtgatgt tagctttgaa tgattaacct tgttatatct ttaattaatt atagaacagt
     3181 ctgggactaa gtatgtctca ggctttttct gttatgtttg aaaatgaaag gaaaaactgt
     3241 tgtgttgata aatatagtat aggttaataa attaacacgt agagatataa tgatgaatcg
     3301 taattaatcg ccttccttat atgaatataa tataaatatc aaaaatattg tgtataaggt
     3361 tgaaaacaag aaaatcatca cctataatac atactaaact tatcggaaat gagaggttaa
     3421 gattatgtgg atgattttta gtggcttaat tgtcggggga ctcttaggtt ttgtcatgca
     3481 aagaacaaga ttttgtttaa caggtggctt tcgagatatg tatgtgcaaa agaataataa
     3541 gatgttctat gcattattaa tcgctattac tattcaaagt ataggattat tgattttgac
     3601 ggcaacagat attttacaaa ttcctgcaca tagttttcca atattgggaa caattatagg
     3661 ttcttttatt tttggaattg gaatagtatt ggctggagga tgtgcaacag gtacttggta
     3721 tcgcgctggt gaagggctaa ttggtagttg gattgcatta gtattatatg ctgttactgc
     3781 agcaatcact aaaacaggga ttttaaagcc agtaatggat aaaattaatc aaccaacgaa
     3841 tgtaaatagt gatatgtctc aaacaactgg cattccgttt tggggattag tcgttatatt
     3901 aactataatc accatttttc tagttgtaag aacactcaat aataagaaag ttcgagttgc
     3961 tgttccaaaa ctaaagcaac gttatacagg tattagatat taccttttcg aaaaacgata
     4021 ccatccattt attgcagcaa ttgtaattgg acttatcgca ctcttagctt ggccaatgag
     4081 tgcatcaact ggaagaaatg acggtttagg tataacaacg ccttcagcaa atttagtaca
     4141 ctttttgatt acaggtgaaa ctaaatttat tgattggggt gtctttttag ttctaggaat
     4201 tttcattggt tcatatattg cagctagagg atcaagagaa tttaaatggc gattgccaga
     4261 caagattaca atacgaaaca gtgccattgg tggcatatgt atgggatttg gtgcgtcagt
     4321 tgctggtggt tgttctatcg gtaacggttt ggttgaaacg gcaacgatga cttggcaagg
     4381 atggattgcg ctagcatcga tgatagttgg tgtatggaca atgagtcatt ttatctttgt
     4441 tcgtccaatg aaaaaagtac accaacaatc tgcaaaggtt aaacagcaaa cgcaaatagt
     4501 atagaagatt attatgcaaa tgatgttgat caaataaaag tgattggaaa aggagaaata
     4561 attatgatac acgaattagg tacagtagga atggtatgtc catttccgtt aattgaagcg
     4621 caaaagaaaa tggcaacatt gcaatctgga gatgaattaa aaattgattt tgattgcacg
     4681 caagcgacgg aagccattcc aaattgggct gcagaaaatg gttatcctgt aacaaactat
     4741 gaacaaattg ataatgcttc atggacaatt acaattcaaa aagtttaacg ttatcatttt
     4801 aacaataaaa tagatattag attctatggc tacttccgct aatttaaaag tgagtaagta
     4861 gtcttttttg ttttagttca tgaaatcatt tttatatagt gtggcacatt ttattccaaa
     4921 agatgtaata aaacttaacg catttttgct ttttataaat tgtcagatta ttatgaaaaa
     4981 aaggagtggt aagtatgaat cttaacgata cgatatttat gtttttgtgt acattattag
     5041 tttggttaat gacaccagga ttaagtttat tttatggtgg gttagttcaa tctaaaaatg
     5101 cgcttaatac tgtcatgcaa agtatggcag caattgtgct tgttacattt gtatggataa
     5161 cagttggttt tacaattagt tttgggaatg ggaatttatg gttcggaaat tgggaatata
     5221 cttttcttaa tcatgtaggt tttgcgactc aagaagatat tagcccacat attcctttcg
     5281 ctttgtttat gttatttcaa atgatgtttt gtacgattgc aatttctatt ttatctggtt
     5341 caatcgctga gaaaatgaag tttattcctt atttattatt cgtagtaata tggactgctc
     5401 ttgtatacag tccagtagca cattgggttt ggggcggcgg ttggattaac aaactcggtg
     5461 tattagattt cgctggaggt acggttgttc atattacatc aggtgtttct ggtttagtat
     5521 tagctattat gattggaaaa ggaaacaaac attctgaatc aacaccacat aatcttatca
     5581 ttacgttgat tggcggtata ttcgtgtgga ttggttggta tggatttaat gtaggtagtg
     5641 cttttacatt tgataatatt gcgatgcttg catttacaaa tactgtcatt tcagccagtg
     5701 caggtgctat aggttggtta attttagaat atatttttaa aaagacgaca agtttacttg
     5761 gacttttact cggtgcatta gcaggattag ttgtcattac tcctgcagca ggatatgtaa
     5821 catatcttag tgcaacaata atggctttaa taggaggtat ctgttgttat attgtcatta
     5881 attacatcaa ggtaaaacta aaatatcatg atgcattaga tgcatttggt attcatggtg
     5941 ttggtggtat tattggtgct gttttaacag cagttttcca aagtaaaaaa gccaatcctg
     6001 acattgagaa tggctttatt tatactggtg acatacatat tatacttgta caaatattat
     6061 gtgtaacagc agttgtaatt tttagtatag tcatgacgtt tattattgcg aaagtaatta
     6121 aattaattac accattatct gttacggaac aagaaacgaa tataggatta gacaagattg
     6181 ttcacggtga acatgcttac tttgaaggtg agctaaatag attcaataaa catattcgat
     6241 attagaatat atttacatag aatattcatt gtcctgacat ttaactaaag gttgatgttg
     6301 ggacattttg ttatacaaaa gttttatttt gaaatctttt tatgaaagaa gcagaaatat
     6361 tatttaaagc ggttacacat atgctaaaat aaggctaagt gtcacaaata atgataggtg
     6421 aataagtatg aaaaatatat ctgatattgc caaattggca ggcgtttcaa aaagtacagt
     6481 atctagattt ttaaataatg gatctgtcag taaaaaaaca agtgaaaaat taacaagaat
     6541 tatagcagaa catgactatc aaccgaatca atttgctcaa agtttaagag cgagacaaac
     6601 acaccttatt ggtgccatta tacctagaat gaattcatat gcagtagatg aaacaatcaa
     6661 aggattggca aaacaatgcc aaaaatatga atcacaatta attttaaatt acacaggttt
     6721 aaatatcgaa gcagaaatac aagcgcttga aacattagca cgcagtaaag tagatggtat
     6781 tgttttaatg gctacagaca taacagagag acatattgaa gtcattaata aaatgaatgt
     6841 accaatcgtt attgttggtc aacaacatga acaacttcat agtattgtgc atgatgatta
     6901 taaagcaggt caaattatag gcgaatggat tggtcaacag ggatatcaac aagttgaagt
     6961 gtttagtgta agtgaaaaag atattgcagt tggtatacat agaaaacgtg gtttacttga
     7021 ccagttagct aaataccaaa ttaaacctaa tatacatgaa acaaatttta cttatgtgga
     7081 agcacaaaaa gatgttgcaa atgttttgga aaatgtggag caagtagatg cggttgttgg
     7141 agcaactgat acgattgcat tagctgccta taaatattat tctgataaaa aagatgttat
     7201 gaaaccacat caaatatatg gttttggtgg tgacccaatg acacaattag tgtctccatc
     7261 gataaaaaca attcattata attattttga agctggccaa tgcgcgatgg aagagataca
     7321 acagatgctt aaaaagcaag atatgccata tagcgtcaca gtagatgtta atatttagac
     7381 gctgtatttt ttaaaataaa tgtggaaccg ataccatata actataaatg gataggttaa
     7441 aagttaaaga acgtaggtaa aatttgctat aatagaatat aaattgttat tagcataaat
     7501 tataaaagga ggactgggta aatattatga ccgaatggac tagagaagaa cgttatcaac
     7561 gaatcgagga cgttgatact gagtatttta aaacattaaa acaacaagtt gatcaatcaa
     7621 aatttcgtca acaatttcat atacaaccag aaacaggctt attaaatgac cccaacggac
     7681 ttatttttta taaagggaag tattatgttt cacatcaatg gttcccatta ggcgcagtac
     7741 atggcttaaa gtattggtat aactacacga gtgatgactt aataaacttt aaagctgaag
     7801 ggccaatttt aaatccagat actaaatatg acagccatgg tgtatatagc ggtagcgctt
     7861 ttgaatataa cgggcattta tattatatgt acacaggaaa tcatcgagat aatcattggc
     7921 aacgacatgc gagtcagatg atcgcacgat tgaaagaaga cggttcagtt gaaaagtttc
     7981 caaagccagt aattagccaa caaccagaag gatatacgag tcattttaga gatcctaaag
     8041 tttttaaata tgatgagaaa tattatgcaa tcattggtgc acaaaataat gatcagcaag
     8101 gtcgattatt actttataat actgaagata taattaattg gcattattta ggtgaaataa
     8161 atacagagtt ggatgatttt ggatatatgt gggaatgccc agattacttt aatgtagata
     8221 atcaagatgt catacttatt tgtccacaag gtattgaacc aaaaggcgat cagttcaaaa
     8281 atatttatca aagtggttat atacttggaa agtttgatat tgaaaagtta acatatgaac
     8341 atgaaaattt tgtcgagctt gataatggtt ttgatttcta tgcacctcaa acatttttag
     8401 atgaaaaagg ccgacgagta ctaattggat ggatggggtt accggaaatc gaatatccta
     8461 ctgataatga aggatgggcc cattgcttaa caattcctcg tgtattaaat gtagaaaatg
     8521 gtcaacttaa gcaacgtcca tatccagcgt tggaaaaatt acgtcacaat aaagagacag
     8581 cattaggcta cgcaaataaa tttactcgaa aattacatcc gtatgaaggt aaacagtatg
     8641 aattaatcat agatattttg gataatgatg ctaccgaagt gtactttgaa ttacgtacat
     8701 ctaagacttc ttcaacatta attgcttata acaagcgtga aaataaaata acattagatc
     8761 gcagcgacag tggtttattg ccgacaaatg ttgaaggtac gacgcgtagt acgatattag
     8821 acacgccatt aaagcaatta caaatttttg ttgatacatc tagtatagaa attttctgta
     8881 atgatggaga acgtgttttg acatctcgaa ttttcccgac agaagacgca ctaggtatta
     8941 aaacatcaac agaatcaggg caagtatatt tacaatttac taaatatgat ttaaaggatg
     9001 aacataaatg agacgtctat tttcaatagg agaagcgtta attgatttta ttccaaatgt
     9061 tacaaatgcg aatttaaaag atgttcaaac atttacgaag caaattggtg gcgcgccatg
     9121 caatgtagct tgtacagttc aaaagttagg acaacaagcg tatatgatta cacaattagg
     9181 taatgatgca tttggagata gcattattga aacgatatca agtattggtg tagatgtatc
     9241 aaaagtgtat cgcacgaatg aagcaaacac tgcgttagct tttgtgagtt tgactgaagc
     9301 gggcgagcga gatttttctt tttatcgtaa gccctctgct gatatgttat ttgaaccaag
     9361 ttttgtgaat gatattgatg tgaatgaaaa tgatgtagta catttttgtt ctgttgattt
     9421 ggtcgatagt ccgatgagag atgcacatta tcaattgatt accaaaacat taaatgcaaa
     9481 tggtactgtc gtatttgatc ctaatgtaag attgccatta tgggataacg ctgaagattt
     9541 aagacaaact attcatacct ttttgccatt ggcgcatatt gtaaaagttt cagatgaaga
     9601 attagaattt attacaggta ttcatgatga aaatgaagcc attcaatcat tatttacagg
     9661 taatgttact gtagtcattt atacgaaggg agcagatggt gctgccgtat atttgaaaaa
     9721 tggtattaat cattatcata gtggctataa ggtaaaacct gttgatacaa ctggggcagg
     9781 ggatgcattt attggtgcag ttattagcag gattttagca accgatgtgt taaatttaac
     9841 tcagttattt gaaaatgaag gtgaggaaat tttagcgttt agcaatcgcg ttgctgcaat
     9901 agtgacaacg aagtatggtg ctattaatag tttgccaaca ttacaagagg ttgaacaagc
     9961 attttaaata attcatttat aaaacagtgt aggaaacaag ttgaatcttg tgctacactg
    10021 ttttttaaaa caaaataaga ttcacggagt aggaaattat agcattttat ttatggaaac
    10081 tgtcaatttc aaatttagta tattttgtta aaaattgcgc cataggattg tagagtgaaa
    10141 tagaaattga taaattatgt agtaatagga ataggtatac aaagagacgt tacatcattt
    10201 tttaattcac gcgtcatatt taattttgtt aataaaatta aaaagccagc tatacagtgc
    10261 atttgctagt tatcttgtta aaatccaaca agatttacaa ttgaatacgc cgttaactga
    10321 ctttattatc ttattatatt tttttaacgt ttctcaccga tgcatagcag tgttctttat
    10381 ttttaaaata gacaattcgc tctttcgaat ctatagattc aatattatgg cgattgacga
    10441 caaagctatt atgacatcta aagaaacgat catctaattg actcagttct tttaaattac
    10501 cataaaattc aatttgacgg ttatctaaat gggcaatgag tctgtgagat tttgttgatg
    10561 attcaaaaaa cataatatca tcatattgaa catacactga attactgcca cgttttaatt
    10621 caatcgtttc aacgctatta tctttagaca acaattgtaa gcgtgtatgt gcagtttcta
    10681 aacagtctat aattcgagtt cttaattcag ctggatcatc tttaaaaata aaatccatcg
    10741 ctgcaacttt gtagacaaat gttaaatagg taagttcact gtgactcgta acgaaaataa
    10801 tgttaccaac tgggtcatgc ttacgaattt cactgcctaa tttgatacca ttaatatcag
    10861 ttgaaagttg aatatctaaa aagtaacagc ctatgtcatt catattttta gcttgctcaa
    10921 gcacctcata aggattatca gttgcgaggg caatttccat aggcttttct tctatcatta
    10981 tataattttt aataatggta accatgtttt ctctttgttt tggatcgtct tcgcaaatga
    11041 aaattttcat acattcacat ccttatggct agttgttaat aatttcaact ttttgaataa
    11101 agaaaccatt ttcgataatt gtatctaata agacattgtc tgcattatca gcaatttctt
    11161 ttaaagttga tagacctaaa ccacgacctt cacctttagt agaaaaactt tcttggaaca
    11221 attcatgaat gcgtggtata tcatcagcgc atttattcat aacaataaac gttactgaat
    11281 tttcactttc aataaatgca acgcgaatga tagggtcatc aatttcagtt gatgcctcaa
    11341 ttgcattatc aagaataata ccaatactgc gacttaaatc gatcatattc aagttaatgc
    11401 tacttacttc atcgggtatt tcgatactaa tcggaaaatt catttcttgt gcacgtaaaa
    11461 ttttcgcagt aattaagcct ttaatttcac gtactttaag attctcgata ccatttaatt
    11521 ttatagcatt catttgtaaa ttgtctttca taggtacaat atttttattg aaataatcac
    11581 gtaggccagg catgtcatct tctcgaatgt attctgaaag tgtcgttaag atattgacat
    11641 aatcatgacg gaacttgcgc atttcgttgt tgatagcttc aatcttcaat gtatattcat
    11701 aataggtttc aatttcttct tgattacgtt tatatttcat ctctttaagg agaaattgag
    11761 aaataacaaa tgttaatata cttaaaaata tagtgatacc aataaaaata aaagaatact
    11821 gccttattac tttagcttca tccgagttta tttgtgaata aaagaaaaat aatgaaaaag
    11881 taagcagtaa gatagtcgaa ataactatta aaaatccttt gtttagtatt agatatggtg
    11941 tgctaatttt tttgagaact ctatttatta tatatgagaa tagtatacta atagtcacat
    12001 aaactacaaa aaagctaggg aatattacaa atatactatc agaaatttt
//
LOCUS       DALAAR010000073        11482 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000073 DALAAR010000000
VERSION     DALAAR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.075"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(47..1303)
                     /locus_tag="O2458_002176"
     CDS             complement(47..1303)
                     /locus_tag="O2458_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCZ3888026.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1625..2104)
                     /gene="rlmH"
                     /locus_tag="O2458_002177"
     CDS             complement(1625..2104)
                     /gene="rlmH"
                     /locus_tag="O2458_002177"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCZ3888027.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2472..4790)
                     /gene="adsA"
                     /locus_tag="O2458_002178"
     CDS             complement(2472..4790)
                     /gene="adsA"
                     /locus_tag="O2458_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCZ3888028.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(5018..5818)
                     /locus_tag="O2458_002179"
     CDS             complement(5018..5818)
                     /locus_tag="O2458_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ3888029.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6208..6996)
                     /locus_tag="O2458_002180"
     CDS             complement(6208..6996)
                     /locus_tag="O2458_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCZ3888030.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6997..8331)
                     /locus_tag="O2458_002181"
     CDS             complement(6997..8331)
                     /locus_tag="O2458_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888031.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8324..10150)
                     /gene="walK"
                     /locus_tag="O2458_002182"
     CDS             complement(8324..10150)
                     /gene="walK"
                     /locus_tag="O2458_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCZ3888032.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10163..10864)
                     /locus_tag="O2458_002183"
     CDS             complement(10163..10864)
                     /locus_tag="O2458_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ3888033.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3250 a         2232 c         1700 g         4300 t
ORIGIN      
        1 ctatttttat tagatcacaa ttttatttta gctcttgttc aagtaatcat ttttcgccaa
       61 aaactttata ctgaatagct tctacattaa atactttgtc aatgagatca tctacatctt
      121 taaattcaga ataatttgca tatggatcta taaaataaaa ttgtggttct ttaccggaaa
      181 cattaaatat tcttaatatt aaatatttct gcttatattc tttcatagca aacatttcat
      241 ttagcgacat aaaaaatggt tcctcaatac tagaagatgt agatgtttta atttcaataa
      301 atttttctac agctttatct gtatttgttg gatcaaaagc tactaaatca tagccatgac
      361 cgtgttgaga gcctggatta tcatttaaaa tattcctaaa ctgttctttc ttatcttcgt
      421 ctattttatt atcaattagc tcattaaagt aatttagcgc taatttttct ccaactttac
      481 cggttaattt attctcttta tttgattttt caatttctga atcattttta gtagtctttg
      541 atacaccttt tttatatttt ggaattattc ctttaggtgc ttccacttcc ttgagtgtct
      601 tatctttttg tgctgttcta atttcttcaa tttcgctgtc ttcctgtatt tcgtctatgc
      661 tattgaccaa gctatcatag gatgtttttg taacttttga agctaattca ttaaatagtt
      721 ctaaaaattt ctttaaatcc tctagcatat cttcttctgt gaatccttca ttcaaatcat
      781 aatatttgaa tcttattgat ccatgagaat atcctgatgg ataatcattt tttaaatcat
      841 aagatgaatc tttattttct gcgtaataaa atcttccagt attaaattca tttgatgtaa
      901 tatatttatt gagttcggaa gataaagtta atgctctttg ttttgcagca tttttatccc
      961 gcggaaacat atcacttatc tttgaccatc cttgattcaa agataagtat atgccttctc
     1021 cttccggatg aaaaagatat accaaataat atccatcctt tgtttctttt gttatattct
     1081 catcatatat tgaaatccaa ggaactttac tatagttccc agtagcaacc ttccctacaa
     1141 ctgaatattt atcttctttt atatgcactt ttaactgctt gggtaactta tcatggacta
     1201 aagttttata tagatcacct ttatcccaat cagatttttt aactacatta ttggtacgtt
     1261 tctctttaat taatttaagg acctgcataa agttgtctat catttgaaat tccctcctat
     1321 tataaaatat attatgtctc attttcttca atatgtactt atttatattt taccgtaatt
     1381 tactatattt agttgcagaa agaattttct caaagctaga actttgcttc actataagta
     1441 ttcagtataa agaatatttc gctattattt acttgaaatg aaagactgcg gaggctaact
     1501 atgtcaaaaa tcatgaacct cattacttat gataagcttc ttaaaaacat aacagcaatt
     1561 cacataaacc tcatatgttc tgatacattc aaaatccctt tatgaagcgg ctgaaaaaac
     1621 cgcatcattt atgatatgct tctcctcgca taatcttaaa tgctctgtac acttgttcaa
     1681 ttaacacaac ccgcatcatt tgatgtggga atgtcatttt gctgaatgat agtgcgtagt
     1741 tactgcgttg taagacgtcc ttgtgcaggc cgtttgatcc gccaatgacg aaaacaaagt
     1801 cgctttgccc ttgggtcatg cgttggttca attcttgggc caatccttcg gaagatagca
     1861 tctttccttg tatttctaat gtaatgactg tggattgtgg tttgattttg gctagtattc
     1921 gttggccttc tttttctttt acttgctcaa tttctttgtc actcatattt tctggtgctt
     1981 tttcgtctgg aacttctatg atgtctatct tggtgtatgg gcctaaacgt ttttcatatt
     2041 ctgctatggc ttgcttccaa tatttctctt ttagtttccc tacagctaaa atggtgattt
     2101 tcatgtcgtt tggtcctcca aattgttatc aactttccag ttatccacaa gttattaact
     2161 tgttcacact gttccctctt attataccaa tattttttgc agtttttgat attttcctga
     2221 catttatgcc cgatttttag tgtataacct gtgtatttgt ggataactct gtgggcattg
     2281 tgggtatttc tctttaaatt gtacacaatt ctggataact atccccattt ctgtggataa
     2341 acaccttgat gtcttattac ttatcctcgt ttttatatcc catatttatc aacggttatc
     2401 gctttttttc ataaaaaaac actaccgtac ctcttattta agaaatacag tagtattatt
     2461 ttcaatatat tttagctagc ttttctacgt cgtacagtcg cgattaaacc tatacctgct
     2521 aataatacaa acatcgcttc tgggcttgaa ctttgattag ttccagtttt tggtaactgt
     2581 ttctcatgcg cgctaccttt aggcactgac attctagcca attgtttccc actcttgctt
     2641 gatacagtag ctccttctat tgtgcgacca gaaccttctg taccgctact aacagttcct
     2701 ctatgcgcta gcaacaatac aactttacct ggagctggtt ttttcgctgg atccatcact
     2761 ttgtcgtcac caattggttg tgtatcttta ccagacttac tacctttgct accttgttgt
     2821 cctttagctg gttgttcact tactgctggt ttacctaata acatacgttg tggttctgtc
     2881 gtatcatact tagctaagtt agctgttttt aaataacttg ctagtacttg atctaatgaa
     2941 ataccttctt ctctaggacc accgaacata ctatatccgt cgccacctga tgctgtgaag
     3001 tcattcatcg ttacgtgata tacacgtttt aaatcaatat tttcaaactt acctgtctct
     3061 ttatttaaaa tttgaatagc attaattcgt ttgccagacg gtttatttat atcatagtaa
     3121 acacggattg aatcagagat atgtagtaaa ccgccattcg ctgttaacac tgtcttaccg
     3181 tccttttgtg ttgttggtgc gcctaaacta tgttcgaaag ccgtccagac gtctgaacct
     3241 tttacatcaa tttgcgcaat cgtatttcca aatggtaata ctgagattaa atcatagcgt
     3301 gtcaccttac cttttgcgat agaggcacga atacctccac catttgtcac ggcaaagtca
     3361 gtctttttag agaaattctt aacgccatac gcttccatag catctgcaat cgcgtttcct
     3421 aaatttgttt cacgcgttct aacgtcatct ctttctcctt tgaaatcaat ggtattgttt
     3481 ggaataatta cctctgcagt ttgtgctcta aatgtttgat cagcttgatt aatttgttca
     3541 gctaatgctt tgttcggtgt tacattttca acgtctttaa cattaatcaa tgacggttta
     3601 atattcgata cctctccatt gcgataatta aatgtaatct taccgatatt cgcaagtgct
     3661 gtacctgttt gtgccaatgc atcattgtta taaatttgac cattttgaag tactgtatgt
     3721 gaatgaccat caataactgt aatacgtttc ttcaattgtg gattttgact taattgtttc
     3781 actaagtaat caccacgcca tgtttcttgt gttgaaggat caattcctaa atgtgatata
     3841 acaacaaatg tatctacgtc tttataaata cgcatcattt ccgctgtcac actttgtaat
     3901 ggatctctaa attcaacgcc tttaatgcct tcaggtcttg ttttcgtctt tgtttctggt
     3961 gtcgttacac caataattcc ataacgaata ccattttttg ttacaatcgt tgaaggctta
     4021 aacgcgcgtt ttccatcttt ataaacgtta gtacttagca tcgggaagtc taacataccc
     4081 tctaactttt tcaactgatc gtatccaaag tcaaattcat ggttaccgac tgccatagca
     4141 tcataaccta ctgcattcat tgctttagcc atttcttcac ctttagactg gtttgaaagt
     4201 ggtaaacctt ggaaggcgtc tcctgcgtct aacattaaat caggcttttc ttgttctttt
     4261 actgttttta atttagccat accgatgaca cgcccttttt cttcggctag tcggccatgg
     4321 atatcatttg tatgtaatat tttatgtgtt gttgtttgtg gtgcattagc agcaaacatt
     4381 cgtggtgaaa gtgatgctgt tttagcattt gataatttga acgttgctgt gtgagttggt
     4441 ttttgtgttg aggcttgttg tgtatctacg tcgcgtgttt cgtttacttt tgttgaaact
     4501 actgtagatg gtttgttaga tggtgcgttg gatgttactt tgctgtttgt atcatcagct
     4561 gttccctgcg ctgatgctga tacgttggtt gccgcttctt tgccagaatg atgagccgct
     4621 tcctttgttg gcggtacttg ttgcttatct gttgttgctt tgtctatcgt tgttactgca
     4681 tgtgctttca ttggtgtatg ctgctcagcc gcgttcgaga cttgccataa tcccattaca
     4741 ctaaaaagca aaacgagcca tacacttgtt ttaagtaata aagctttcat ttcgttccct
     4801 ccattaactg tcatacacca atatattatc ataatcctta tattaaaatg attgatttta
     4861 tgtaaattat ttataaatta catttaatat cgaattattt tacacatttc aatcaagccg
     4921 attcaactca acaacagcca tttcccacca acaacataaa aaagcacccg ataaagtcgt
     4981 ctcacagcaa tgcggaactt tatcggatga ctctcattta tattgtatat attggcgttg
     5041 gaatagcttt atccgtatca catagcaata cttctttttc cgtatcaata tcgtgttcgt
     5101 tcaatacttg gccaacactc atacgcgcca aatctttcat gttattgtct tgtgataaat
     5161 gcgataggta aatacgtttc gtgttacctg taatcacgtc tgtcattgca tgagccgcat
     5221 cctcattaga tacatgaccc atatcgccta aaatacgttg tttcgtcttc catggataac
     5281 gacacattct caacatatcg acgtcatgat tactctcaaa aataaacgca tcgctgccac
     5341 gtatcatacc tttcatacga tcagacacgt aacccgtatc cgttaaaatc gtaaacttct
     5401 tatagttatt atggaaaata taaaattgcg gatctattgc atcatgtgac acgttaaacg
     5461 attcaacatc gaaacctgca atagattttg tttcataagg attaaaaatg aatttctgat
     5521 ccatagggat gcgactatct ttcttttcaa ttgcctgcca agtcttttca ttcgcataaa
     5581 ttggcaattg atatttacgc gccaaaacac ctaatccttt aatatgatca atatgttcat
     5641 gggttactaa aataccattt aaatcttgaa tattacggtc aatttgacta aacaattctt
     5701 ccattttctt tccagtcaaa ccaacatcaa ctaatagact acctttttca ttttctacaa
     5761 aagtggcgtt acctgtacta ccacttgcta atacactcat gcgtatcaag cggctcatcc
     5821 tttctcttta aacagtcaat ctctctatat gactgcaata tacatcagtg caaatatacg
     5881 attttcccta cagaaactat cagtatatta gctgttttgt atacctttcc tattatgtaa
     5941 ctttagttca tcgaccggtt tcttatattt aaattttgat aatcacgtgc aatgaatttc
     6001 ttaattatta taacaatgat tagacaaata ctaaagtttt taaaataaca gttacactaa
     6061 ttttacaatg cggttctact taaaatatga ataaatgcgc acgttggaga cgcaaaaaca
     6121 tcagccataa taaaccgtac cagcaaaaaa gctagcaccg cttctcacat atgatgagct
     6181 tgcaatgcta gcttattacg attcatatta atgattaata attttagggt tattatttgt
     6241 cgcttcgaca tagtaagtat tcgttttatc cttaccgtca tgcttcactt taatttccca
     6301 gtttggttgt aacaattgaa cattcgtttc attcacgact gagtagtaac cgagtctagc
     6361 attaatgact tcatcacctt ttttcaagta acgattataa tataatgcct cgattgcttt
     6421 tctcacacca atcacttgat gcttcttatc tgcaccatct gtgggcttaa tgtcatccat
     6481 cattgattgt ttataactag tcgctttgtt atcttctata ttaaagttca acatcgcttt
     6541 actgttattt aaaatcggaa aatcatcata cgtttgttca tattttacag aatctgaact
     6601 aatctcgctt aattgatatt cagcaccttt aaatacgcgc ttgttaacat aatctttcaa
     6661 atcttttaag ttattgtcac tgacctttac cgattgacta atgttcgcat tcaataattt
     6721 tccaccatct gatgtggtca aatccgaatc gcctttagct ttagaactaa agtcttttga
     6781 tcgccctgta atttgctcta attttatacc tttaactgat ttattcaata tactagtcgg
     6841 tactttaatt tcttcttgct gaaaattaac ttcattgtta ctctcgactt cattaatgtg
     6901 tgagcgattg actttattaa cataaatcga tactaacacg atgttgacaa gaataaacac
     6961 gaaaatgaaa agtgtctttg tcagtttcca gttcatttat tcaagcctcc catcgttata
     7021 aacataccat tcgccatcat attctacata ccaacgcggt acgagttcac tgttaatctg
     7081 cacttcaatg tgattatgat ctgaattatc ctgcatttcg taaccgatag cgatgtttgt
     7141 tactttttca aaattaatat cactattgtt cgctaagctt gaacgtacag actctaattt
     7201 cggcaacgat ttattatcct cactatttaa aacaacgtcg gtgcgtaata acgaacgacg
     7261 atagtcaaag acgccttttt caccccaagt gacttgaatt tgattagaac cttctttatt
     7321 aaacgttgga taaccattaa ggaaacgttg atatgttaac tcgcctgact gattattcgt
     7381 actaaacaat ctaaagtctt cgtttaagaa accaccatga ccattaataa aatcaaaggt
     7441 tcctggaatc gtttcttcca ttttgctgga actcgcttca tcttcggaca ggtttttata
     7501 atgatatttt tcatttttat cgttatagtt tgcgacacct gtattattgt tgtaggttgt
     7561 aacaccactc tttgaactac gaacgatggt tgaatcgtca aatagtatag cattcatttt
     7621 ctcaacacta atcgtgttaa ataccatgcg atatgttttt aacttttcag gtttacttgg
     7681 tgcaaaaaca tgcgtcgtac gatcaattgt atctttgttt gtgatgatat ctgtgtatgg
     7741 ttgcatatct tttttcactg ctgctaatgc atctaaaaaa tgatcatttt tcgttgtagt
     7801 tgttaatttt acgtaatcgt ggcgatcttt gcttatagca taaagcacga tattatcatc
     7861 agcatcatga tctatgacca aacgattgaa attgaaatga tttggtactt tcgcattcat
     7921 gttcagtact tgaccaagat atgttgataa cggtaaatca tacgtaaaat cgaataatat
     7981 aaaatcactg ttcaaatcag gaatcatcaa gttatgatca cgacgaacat gttccacgga
     8041 cttcacttct ttatttttca aaggtttcgt cagtttattc acattagata ccgtcgcaat
     8101 cgttccttct ggatgatcat ttttcgaatg aataatctga aatggcgtaa tagttgtatc
     8161 cattttggct gtcataggtg tcgttaaagg tttcgtttca ctcttcttac tatctgtatt
     8221 gtcgacattt gcaatatcag gagaaaagtt ccataccata tatgtcaata cgacactcat
     8281 caagacgagt agtgctaaaa tgacagattt aatatgctcc ttattattca tcccaatcac
     8341 cgtcttcaat gacttcacat ggaagtgtga taaagataga tgtaccttga ccttctacac
     8401 tgtttgccca aatacgacca ttgtgcgctt ccacaatctc tttcgaaatg gctagtccta
     8461 atccagtacc acccatttta cgcgtacgtg ccttatctac acgatagaat cggtcgaata
     8521 tcttatcgac tttattgata ggaataccaa tgccattatc tttaatacga atcgtcattc
     8581 gattataaag tggattttgt ttcacgtgga actcgacacg tttatcgcct ctagaatatt
     8641 tcatcgcatt tgtaatgaca ttatcaaata cttgcgtcat tttatcagga tcaaattctg
     8701 tgaaaatcgt ctttttcgga atatctcgaa taaatgttgt atctttcgca gacatttcat
     8761 gtcgattaat aattttatta atgaacatgt taaagtcgat aatttctttg ttgatttgat
     8821 cagactcatt atccatttta gataactgta gcaagtcatt gaccagtcga atcattcgtt
     8881 ctgtttcttc acgggtaaca gataaaaatt gtggcgcaag ttcctcatct ttccatgcac
     8941 cttcttcaag tgcttcaatg taactattca tagaagttaa aggtgtacgt aactcatgtg
     9001 atacattggc aacaaattca cgacgctcac gttcaacttg ttgttgttca gttacgtcat
     9061 gtaacacagc gatataacca gttacaaatc ctgtttcctg cacaatcgta ctaaagttaa
     9121 cacgtgcgat tagaccttct tcttcattta aatctaataa gaaactatca ttattctctt
     9181 gaatttcttc cagtttaaat tcatcttcaa gacttaatac acttaacatg taatatccga
     9241 tgatgtcttc tttcgccata ccaagcatct tgagtgccat atcattgacg atacgaatac
     9301 gtccacggcg gtctgttgca ataataccat cactcatatg ggtgataact gagtccagtc
     9361 tacgtttctc actttcagta ttagcctgcg cttcttgtac acgtttagac aagttattaa
     9421 atgctaaagc taattcgcca atttcatcat taccataaat cttcacacgt tgcgtatagt
     9481 tacctctgga catttcgacc gtctggttac gcatatcggt gattggtttg gtaatcgttc
     9541 gcgctataaa gaatcctagg atgactgtga ttaataatga aatagctgta ccaacaatga
     9601 atatttgatt tatattattt aattggttat aaacgtcatt aatttttgat tcgatataaa
     9661 tattaccaat taccttttta tcgactttaa ctgggatatt atatacccag acacggtcct
     9721 taccaccgcc ataatctttt aaaattaaat gatcgtttga ttgtcctagt gatagtgctt
     9781 tttggacaga actatcattc gctttttgat tgattagact acggttagac tgcttcgtcg
     9841 tcgcaataat aatttggtct ttatctataa aacgaatttc tccaatttct tgacggttgg
     9901 catactcact taataaattt tgaatatctt tttgtgcatt tacggagccc ttttcgtcat
     9961 atactttttc aatactaatt tctaattgtt tcgcgtactg cgtaatattc ttcttaaaat
    10021 tatcaagcag ctctttttca aggttatttg taaaatataa cccgataatt tgcataccaa
    10081 tgataatcag taatacataa acaattacaa gtttagtatg aagggattgt agttgtttta
    10141 gccacttcat tcgtttcgac ctctactcat gttgttggag gaaatatcca acgcctctac
    10201 gcgtcacaat atattcagga tgtgacggat catcttcaat cttttcacgt aaacgacgaa
    10261 tcgttacatc gaccgtacgt acatcgccaa agtaatcata gccccatact gtttgtaata
    10321 aatgttcacg tgtcattact tgtcccatat gttttgataa ataatggaac aattcaaatt
    10381 cacgatgtgt taattcaata tcttcgccac gttttttaat agaatatgcg tctggataaa
    10441 tcacaatatc tttaattgtg atttcattcg ttacatttcc agtgtcttgt gctggttgtg
    10501 agtaatgacg acgtaagttc gctttcacac gtgcgattaa ttcacgcgta ctaaacggtt
    10561 tcgttacata gtcatctgca cctagttcta aaccaagcac tttatcaatt tctgaatctt
    10621 tagcagtaag cattattatt ggcatttcgt attttttgcg cacttcacga catacttcca
    10681 taccatcacg accaggtaac atgatatcta gtaatacgat gtctggttct tcttcataaa
    10741 ttaagtcgac tgcatcatta ccatcgtatg cacagtacac atcgtatcct tcttttttta
    10801 agttaaattc taaaatatca gcaatcggtt tttcatcatc aactacaaca acttttctag
    10861 ccatttgcat aaacctcttt tcttaaatca ttaatattat tttatcattt tttgttcttt
    10921 atcgtttatg tttgtgtaaa aaatcacagg atgtcgctta ctatttttgt tattgtgcgc
    10981 attttccgtc ttataacgta tagtaaccta ttatataatt taccatttat aagctattaa
    11041 ttctattttc caatataaac ttatatatta ctatttttta ttagtttctc acggcggatt
    11101 gtgacaatga tatttaacac aatgatgagg tctctattcc aataataaaa ttaggataat
    11161 ggcaacttct agttgcacgt cattgctcag aaaataaata tcattattcg ttattcgtta
    11221 ctttaacaat tgatgttatg gaggactggt ggcatatcac aacgaagaaa tatgttgaca
    11281 gtagcatatg tttgtacgtc taataaagat tttattgtct acgtttatct ttcatatcca
    11341 accactaagt tacttgaggt tcatacattt taaagaaaat ggacgaacag ataaatcaat
    11401 atgattatca ttcgtccata tatcttttat taatataata ttaaacgcaa aaaaatgcct
    11461 atcctcttgg ataggcattt gt
//
LOCUS       DALAAR010000074        11453 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000074 DALAAR010000000
VERSION     DALAAR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.076"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            314..3019
                     /gene="acnA"
                     /locus_tag="O2458_002184"
     CDS             314..3019
                     /gene="acnA"
                     /locus_tag="O2458_002184"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCZ3888034.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3199..3666
                     /locus_tag="O2458_002185"
     CDS             3199..3666
                     /locus_tag="O2458_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ3888035.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3873..4169)
                     /locus_tag="O2458_002186"
     CDS             complement(3873..4169)
                     /locus_tag="O2458_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCZ3888036.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4545..5153)
                     /gene="plsY"
                     /locus_tag="O2458_002187"
     CDS             complement(4545..5153)
                     /gene="plsY"
                     /locus_tag="O2458_002187"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCZ3888037.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5359..7350
                     /gene="parE"
                     /locus_tag="O2458_002188"
     CDS             5359..7350
                     /gene="parE"
                     /locus_tag="O2458_002188"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCZ3888038.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7350..9752
                     /gene="parC"
                     /locus_tag="O2458_002189"
     CDS             7350..9752
                     /gene="parC"
                     /locus_tag="O2458_002189"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCZ3888039.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10002..>11453
                     /locus_tag="O2458_002190"
     CDS             10002..>11453
                     /locus_tag="O2458_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ3888040.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNS"
BASE COUNT         3997 a         1657 c         2193 g         3606 t
ORIGIN      
        1 tgtcccactc ccatcaaaag aatgacattg aaaaaaatat taaagtttgg tgccataatt
       61 ttctttctgc tcaatgaaat aatacgttta aaaattgaat tgaatcattc gcgaaattga
      121 taattattct caattaataa aaaatactta ttacacctta cctgtatgat aagttttgct
      181 tatatactct gattaaaaag tcaaaaccta aattaagcgt tttctaagca tgatgagttg
      241 tttatcaata aatataataa tacaatttaa tcaaggcata taaatataaa aatgtatcaa
      301 gggggatcat taaatggctg caaattttaa agagcaatca aaaaaacatt ttgacttgaa
      361 tggccaaagt tatacttact atgatttaaa agctgtagaa gagcaaggta ttactaaagt
      421 ttccaattta ccttattcaa ttcgtgtttt gttagaatct ttacttcgtc aagaagatga
      481 ttttgtaatt acagacgatc atattaaagc tttaagtcag tttggaaaag atggaaatga
      541 aggcgaggtg ccatttaaac cttctcgtgt tattttacaa gatttcacag gtgtaccagc
      601 cgtagttgat ttagcttctt tacgtaaagc aatggatgac gttgggggag atattactaa
      661 aattaatcca gaagtaccgg tggatttagt tattgaccac tcagttcaag tggatagcta
      721 tgcaaatcca gaagctcttg aacgtaatat gaaattagaa tttgaacgta actatgaacg
      781 ttatcagttt ttaaattggg caacgaaagc atttgataat tacaatgcag ttcctcctgc
      841 aactggaata gttcaccaag ttaacttaga atatttagca agtgttgtac atgttcgtga
      901 tgtagatggt gaaaaaactg catttccaga tacattagtt ggtactgatt cacatacaac
      961 aatgataaat ggtattggcg tactaggatg gggtgttggt ggtattgaag ctgaagctgg
     1021 aatgcttgga caaccttctt atttcccaat tccagaggtt attggtgtac gactagtaaa
     1081 ttcattacca caaggcgcaa cagcaactga tttagcgtta agagtaactc aagagctacg
     1141 taaaaaaggt gttgttggta aatttgtgga gttctttggt ccaggtgtac aacatttacc
     1201 actagcagac cgtgctacaa ttgcaaacat ggcaccagag tatggagcaa cttgcggatt
     1261 cttcccagtt gatgatgaat ctcttaaata tatgaagtta actggtagat cagacgaaca
     1321 tatcgcgcta gtaaaagaat atttgaaaca aaaccatatg ttctttgatg ttgagaaaga
     1381 agatcctaat tatacagatg ttatcgaatt ggatttatca acagttgaag catcgctttc
     1441 aggaccaaaa cgtcctcaag atttaatttt cttaagtgat atgaaatcat catttgaaaa
     1501 ttctgtaaca gctccagcag gcaaccaagg acacggttta gataaaagtg aatttgataa
     1561 gaaagctgaa attaacttta aagatggatc aaaagctaca atgaaaacag gtgatattgc
     1621 aatagcagca attacatcat gtacaaatac atctaaccct tatgtaatgt taggtgcagg
     1681 tttagttgct aaaaaagcag ttgaaaaagg cttgaaagtt cctgaatacg ttaaaacttc
     1741 tctagcacca ggatcaaaag ttgttaccgg atatttaaga gatgctggct tacaacctta
     1801 tttagatgat ttaggcttca acttggttgg ttatggatgt acaacttgta tcggtaattc
     1861 aggtccttta ttaccagaaa ttgaaaaagc gattgctgat gaggacctat tagtgacatc
     1921 tgtattatct ggtaaccgta actttgaagg tcgtatccat cctcttgtta aagccaatta
     1981 cctagcttca ccacagttag ttgttgctta tgcattagct ggaacggttg atattgattt
     2041 acaaaatgaa cctattggta aaggtaatga cggtgaagat gtatatttga aagatatttg
     2101 gccatcaatt aaagaagttt cagataccgt tgatagtgtt gtaacacctg aattatttat
     2161 tgaagaatat aataacgtat acaataacaa cgaattatgg aatgagattg atgtaactga
     2221 tcaacctcta tatgactttg atcctaattc aacatacatt caaaatccat cattcttcca
     2281 aggattatct aaagaaccgg gtacgattgt tccattaaat ggtttacgtg ttatgggtaa
     2341 attcggtgat tctgtgacaa ctgaccacat ctctccagca ggtgcaattg gtaaagatac
     2401 gccagctggt aaatatttac aagatcatca agtgcctatt cgtgaattta attcatatgg
     2461 ttcaagacgt ggtaatcacg aagtaatggt tcgaggtacg tttgctaata tacgtattaa
     2521 aaaccaatta gcgccaggta ctgaaggtgg ttttacaact tattggccaa caaatgaagt
     2581 aatgcctatc tttgatgctg caatgaaata taaagaagat ggtacaggtt tagttgtatt
     2641 agctggtaac gattatggta tgggttcatc tcgtgactgg gcagcaaaag gtacaaactt
     2701 attaggtgtt aaaacagtta ttgcacaaag ttatgaacgt atccatcgtt caaatttagt
     2761 tatgatgggt gtattaccat tagagtttaa aaaaggtgaa tcagctgatt ctcttggtct
     2821 agatggtaca gaagaaattt ctgttaatat tgatgaaaat gttcaaccac atgactacgt
     2881 caaagttact gctaagaagc aagatggtga tttggtagaa tttgacgcta tggttcgttt
     2941 tgactcactt gttgaaatgg attactatcg tcacggtgga attttacaaa tggttttaag
     3001 aaataaatta gcgcaataaa aaatagatat cacagtaaaa ttttaatcgg tatttgaggc
     3061 actttgatag tgctcaaaaa tacttctata aactttatga tataataggt cggttcaaaa
     3121 cctttgtgtt cttggccggc ctcttcttgt attttatgtt agaatgagat ataaagttaa
     3181 actgaaagaa ggcttaaaat gatatatagt attacagaaa tagaagcgcg ttatgctgaa
     3241 actgataaga tgggtgtaat ttatcacggg aattatgcaa cttggtttga agttgcgcgg
     3301 ttggattata tatcgaagtt aggttttagt tatgctgata tggaaaaaca aggaatcatt
     3361 tcacctgtga ctgacctcaa tgtcaattat aaaaagtcta ttttttatcc agaaaaagtt
     3421 aaagttaaaa cttgggttga aaaatattcg agattacgtt cagtgtataa atatgagatt
     3481 tttaatgaaa aaggtgaact tgcaactaca ggttccacag aattgatttg cattaaagaa
     3541 gatactttta agcctatacg gttggatcgt tatttcccag attggcatga agcttatagt
     3601 aaagtgcaaa cgctcaataa tgaagggaaa acagtagaga taatggatgg tattgattct
     3661 ttataagtta ctgtaaatgt ttggagaggc aatattaaca attaatctat aaaaaatagc
     3721 caatacgtta acattaatgt aattagcgtg aagataagct aatatatagc aaaaacgaac
     3781 ttgttcatac aacatcagat gtgctaaacc aaagtacaac ttccgaggtt caggacacat
     3841 cagaatgtgt aaacaagttc gttttatttt agttattttg tggaataaga aatttcatct
     3901 tcgtgatcaa ctacatttac aataatgtgg tcatcttcaa agtaccacaa atctttttcc
     3961 gctacgacaa catttaaatc gtcatattgt tgttcatagc caatatcaac atcttccttt
     4021 ggttcaactg taaaagcagg actaaatcct tgcttgagtt ggaattcgcc accatatctt
     4081 acaaaaaaca cgagcacttt attattttca ggcaactcaa gttcattttt aaaccaagtt
     4141 actgctgcat cagtaagttc tatttgcata acaatgacct cctatattta ttacagaatg
     4201 ggagtgggcc aacttgtatt atttgtggaa tttcttatcg aaattctctg tgttggagcc
     4261 caccccaacc tgctcattat tgtaagctga atttccataa gcttctatgt tgggaccccc
     4321 gaccagaatt gaaaaaacct agttataagc gcattttgtt cggttaacta cagctaatat
     4381 aacattgtgg agcctatgac attgatttat gtctcggtct caaatgttct tgtaaaaaaa
     4441 ctaagtatag ttgaatttta agttaaaaaa attgtttttg caaaaataaa ataatccatc
     4501 aactgcaaaa aaatatgcaa tgatggacta atgtcaaaaa attgttacat ccattttatt
     4561 ttaggttctt cgcctctaaa aatccttgcg atattagagc gatgtctaat tatcaatatg
     4621 attgaaacta agaaactaac gactaataaa atatagtctt gaatgataag cgagccaatc
     4681 acacagcaaa ttgctgcaac gatacttgct aaagaaacat atttaaaaat cttcaataca
     4741 ataaagaaga taattgcaag tattagtaaa agtatcggat tgactcccaa gacgacacct
     4801 gcactagttg caacagcttt gccaccttgg aattttaaat aaacaggata aacgtgtcca
     4861 agtatagcga ataagccaac aattaaacca tttgtaaaaa aagtactaat agggccatct
     4921 gcgtgaactt gtaaccataa agggaagaaa acagttatga accctttgaa aatatctaga
     4981 aatgttacca agaatcctgc aggacgacct aatactctaa agctattagt agcgccagta
     5041 ttaccactac caaattgtct aatatctttt ttgaaaaata attttccaat tacgaatcca
     5101 cttgggaaag cgccgataag ataacttagt agtaacatga cgattatcat cataaatatt
     5161 acacatcctt taatatctta ggactatttt atcatattca tttaaattac ggctaaaaat
     5221 tttaaaaacg gggattaata tatggaatta agctatgaaa gttaattgat acttgcattt
     5281 tacgctgatt tatataagaa taactattgt atagttttaa aaacgaacgt acgtttgcag
     5341 gaggcgaaat cattggcaat gaataaacaa aataattatt cagatgattc aatacaggtt
     5401 ttagaggggt tagaagcagt tcgtaaaaga cctggtatgt atattggatc aactgataaa
     5461 cggggattac atcatctagt atatgaaatt gtcgataact ccgtcgatga agtattgaat
     5521 ggttacggta acgaaataga tgtaacaatt aataaagatg gtagtatttc tatagaagat
     5581 aatggacgtg gtatgccaac aggtatacat aaatcaggta aaccgacagt cgaagttatc
     5641 tttactgttt tacatgcagg aggtaaattt ggacaaggcg gctataaaac ttcaggtggt
     5701 cttcacggtg ttggtgcttc agttgtaaat gcattgagtg aatggcttga agttgaaatc
     5761 catcgagatg gtaatatata tcatcaaagt tttaaaaacg gtggttcgcc atcttctggt
     5821 ttagtgaaaa aaggtaaaac taagaaaaca ggtaccaaag taacatttaa acctgatgac
     5881 acaattttta aagcatctac atcatttaat tttgatgttt taagtgaacg actacaagag
     5941 tctgcgttct tattgaaaaa tttaaaaata acgcttaatg atttacgcag tggtaaagag
     6001 cgtcaagagc attaccatta tgaagaagga atcaaagagt ttgttagtta tgtcaatgaa
     6061 ggaaaagaag ttttgcatga cgtggctaca ttttcaggtg aagcaaatgg tatagaggta
     6121 gacgtagctt tccaatataa tgatcaatat tcagaaagta ttttaagttt tgtaaataat
     6181 gtacgtacta aagatggtgg tacacatgaa gttggtttta aaacagcaat gacacgtgta
     6241 tttaatgatt atgcacgtcg tattaatgaa cttaaaacaa aagataaaaa cttagatggt
     6301 aatgatattc gtgaaggttt aacagctgtt gtgtctgttc gtattccaga agaattattg
     6361 caatttgaag gacaaacgaa atctaaattg ggtacttctg aagctagaag tgctgttgat
     6421 tcagttgttg cagacaaatt gccattctat ttagaagaaa aaggacaatt gtctaaatca
     6481 cttgtgaaaa aagcgattaa agcacaacaa gcaagggaag ctgcacgtaa agctcgtgaa
     6541 gatgctcgtt caggtaagaa aaacaagcgt aaagacactt tgctatctgg taaattaaca
     6601 cctgcacaaa gtaaaaacac tgaaaaaaat gaattgtatt tagtcgaagg tgattctgcg
     6661 ggaggttcag caaaacttgg acgagaccgc aaattccaag cgatattacc attacgtggt
     6721 aaggtaatta atacagagaa agcacgtcta gaagatattt ttaaaaatga agaaattaat
     6781 acaattatcc acacaatcgg ggcaggcgtt ggtactgact ttaaaattga agatagtaat
     6841 tataatcgtg taattattat gactgatgct gatactgatg gtgcgcatat tcaagtgcta
     6901 ttgttaacat tcttcttcaa atatatgaaa ccgcttgttc aagcaggtcg tgtatttatt
     6961 gctttacctc cactttataa attggaaaaa ggtaaaggca aaacaaagcg agttgaatac
     7021 gcttggacag acgaagagct taataaattg caaaaagaac ttggtaaagg cttcacgtta
     7081 caacgttaca aaggtttggg tgaaatgaac cctgaacaat tatgggaaac gacgatgaac
     7141 ccagaaacac gaactttaat tcgtgtacaa gttgaagatg aagtgcgttc atctaaacgt
     7201 gtaacaacat taatgggtga caaagtacaa cctagacgtg aatggattga aaagcatgtt
     7261 gagtttggta tgcaagagga ccaaagtatt ttagataatt ctgaagtaca agtgcttgaa
     7321 aatgatcaat ttgatgagga ggaaatctag tgagtgaaat aattcaagat ttatcacttg
     7381 aagatgtttt aggtgatcgc tttggaagat atagtaaata tattattcaa gagcgtgcat
     7441 tgccagatgt tcgtgatggt ttaaaaccag tacaacgtcg tattttatat gcaatgtatt
     7501 caagtggtaa tacacacgat aaaaatttcc gtaaaagtgc gaaaacagtc ggtgatgtta
     7561 ttggtcaata tcatccacat ggagactcct cagtgtacga agcaatggtc cgtttaagtc
     7621 aagactggaa gttacgacat gtcttaatag aaatgcatgg taataatggt agtatcgata
     7681 atgatccgcc agcggcaatg cgttacactg aagctaagtt aagcttacta gctgaagagt
     7741 tattacgtga tattaataaa gagacagttt ctttcattcc aaactatgat gatacgacac
     7801 tcgaaccaat ggtattgcca tcaagatttc ctaacttact agtgaatggt tctacaggta
     7861 tatctgcagg ttacgcgaca gatataccac cacataattt agctgaagtg attcaagcaa
     7921 cacttaaata tattgataat ccggatatta cagtcaatca attaatgaaa tatattaaag
     7981 gtcctgattt tccaactggt ggtattattc aaggtattga tggtattaaa aaagcttatg
     8041 aatcaggtaa aggtagaatt atagttcgtt ctaaagttga agaagaaact ttacgcaatg
     8101 gacgtaaaca gttaattatt actgaaattc catatgaagt gaacaaaagt agcttagtaa
     8161 aacgtatcga tgaattacgt gctgacaaaa aagtcgatgg tatcgttgaa gtacgtgatg
     8221 aaactgatag aactggttta cgaatagcaa ttgaattgaa aaaagatgtg aacagtgaat
     8281 caatcaaaaa ttatctttat aaaaactctg atttacagat ttcatataat ttcaacatgg
     8341 tcgctattag tgatggtcgt ccaaaattga tgggtattcg tcaaattata gatagttatt
     8401 tgaatcacca aattgaggtt gttgcaaata gaacgaagtt tgaattagat aatgcagaaa
     8461 aacgtatgca tatcgttgaa ggtttgatta aagcgttgtc aattttagat aaagtaatcg
     8521 aattgattcg tagctctaaa aacaagcgtg acgctaaaga aaaccttatc gaagtatacg
     8581 agttcacaga agaacaggct gaagcaattg taatgttaca gttatatcgt ttaacaaata
     8641 ctgacatagt tgcgcttgaa ggtgaacata aagaacttga agcattaatc aaacaattac
     8701 gtcatattct tgataaccat gatgcattat tgaatgtcat aaaagaagaa ttgaatgaaa
     8761 ttaaaaagaa attcaaatct gaacgactgt ctttaattga agcagaaatt gaagaaatta
     8821 aaattgacaa agaagttatg gtgcctagtg aagaagttat tttaagtatg acacgtcatg
     8881 gatatattaa acgtacttct attcgtagct ttaatgctag cggtgttgaa gatattggtt
     8941 taaaagatgg tgacagttta cttaaacatc aagaagtaaa tacgcaagat accgtactag
     9001 tatttacaaa taaaggtcgt tatctattta taccggttca taaattagca gatattcgtt
     9061 ggaaagaatt gggacaacat gtatcacaaa tagttcctat cgaagaagat gaagtggtta
     9121 ttaatgtctt taatgaaaag gactttaata cagatgcatt ttatgttttt gcgactcaaa
     9181 atggcatgat taagaaaagt acagtgcctc tatttaaaac aacgcgtttt aataaacctt
     9241 taattgctac taaagttaaa gaaaatgatg atttgattag tgttatgcgc tttgaaaaag
     9301 atcaattaat taccgtcatt actaataaag gtatgtcatt aacgtataat acaagtgaac
     9361 tatcagatac cggattaagg gcagctggtg ttaaatcaat aaatcttaaa gctgaagatt
     9421 tcgttgttat gacagaaggt gtttctgaaa atgatactat attgatggcc acacaacgcg
     9481 gctcgttaaa acgtattagt tttaaaatct tacaagttgc taaaagagca caacgtggaa
     9541 taactttatt aaaagaatta aagaaaaatc cacatcgtat tgtagctgca catgtagtga
     9601 caggtgaaca tagtcaatat acattatatt caaaatcaaa tgaagaacat ggtttaatta
     9661 atgatattca taaatctgaa caatatacaa atggctcatt cattgtagat acagatgatt
     9721 ttggtgaagt aatagacatg tatattagct aaaaactata tgcaatcacg aaattaaatg
     9781 ataaaataca gtaatgttaa attttgacta aattcaaggg atttatatta aatgctgacc
     9841 aagtacttat cgttaaatta gcgatatact ttgaccaaac taatataaat ctcttgtatt
     9901 gtataagaaa tgatgatgta tctctatatt tactgaaggt tcaattcggt ctaattttat
     9961 agaaggtaca tgattcatgt agaggatgcg aggggtttat tttgaaagat ttcgatagtt
    10021 taatacctgg atggtttaaa gaatttgtcc atgttggtac cgatttaata tggtctcaat
    10081 atttaattgg tctattattg acagctggat tcttctttac gattagttct aaattcgttc
    10141 aattacgaat gttacctgaa atgtttagag ctttagtaga acgtccagaa actttagaag
    10201 atggtaagaa gggtatttcg ccattccaag catttgcgat tagtgctggt tcgagagttg
    10261 gtactggtaa tattgctggt gttgcgactg cgattgtttt aggcggtcca ggtgcagtat
    10321 tttggatgtg ggttattgca tttataggtg cagcgagtgc atttatagaa gcgactttgg
    10381 ctcaggttta taaagtacat gataaagatg gtggattccg tggtggtcca gcttactata
    10441 ttactaaagg tttaaatcaa aaatggctag gtatcgtatt tgcgatttta attacaatta
    10501 catttgcatt tgtatttaac acagtgcaat ctaatacaat tgcggagtcg ttaaatacgc
    10561 aatataatat tagtccagta atcacaggta ttattttagc aatcgtaaca gctattatta
    10621 tatttggtgg tgtacgtagt attgctacgt tatcttcgtt aattgtaccg attatggcta
    10681 tcatttacat tggtatggtt ttagtaatat tgctatttaa tttagatcaa attgttccta
    10741 tgataggtac gattattaaa agtgcatttg gtatcgaaca agtaactggt ggcgctgtag
    10801 gtgctgcggt tcttcaaggt atcaaacgtg gtttattctc taacgaagct ggtatgggtt
    10861 ctgcgccgaa tgcagcggca actgctgccg taccacaccc tgttaagcaa ggtttaatcc
    10921 aatcattagg tgtgttcttt gatacaatgt tggtttgtac agcaactgca atcatgattt
    10981 tactatattc aggactgaaa tttggtgata acgcacctca aggtgttgca gttactcaat
    11041 cagcacttaa tgagcattta ggttctgctg gaggtatttt cttaacaata gcagttacac
    11101 tgtttgcatt ttcatctgtt gtaggtaatt actattacgg tcaatctaat attgaatttt
    11161 tatcaacaaa ccgtgtaata ttatttatct ttagatgtct tgttgtagta cttgtctttg
    11221 tcggtgcagt tgtaaaaaca gaaacagtat ggaatacggc agacttattt atgggcttaa
    11281 tggcaattgt aaacattatt tccattatag gactgtccaa tgtagctttt gcattgatga
    11341 aagattatca aaagcagaaa aaagaaggca agaaccctgt ctttaaacct gaaaacttag
    11401 aaattaactt atttggaatt agtgcttggg gcgctaacaa atataagaac tct
//
LOCUS       DALAAR010000075        11337 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000075 DALAAR010000000
VERSION     DALAAR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.077"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(80..388)
                     /locus_tag="O2458_002191"
     CDS             complement(80..388)
                     /locus_tag="O2458_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCZ3888041.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(403..831)
                     /gene="ruvX"
                     /locus_tag="O2458_002192"
     CDS             complement(403..831)
                     /gene="ruvX"
                     /locus_tag="O2458_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCZ3888042.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(835..1095)
                     /locus_tag="O2458_002193"
     CDS             complement(835..1095)
                     /locus_tag="O2458_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCZ3888043.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1158..3788)
                     /gene="alaS"
                     /locus_tag="O2458_002194"
     CDS             complement(1158..3788)
                     /gene="alaS"
                     /locus_tag="O2458_002194"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCZ3888044.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(4131..6608)
                     /locus_tag="O2458_002195"
     CDS             complement(4131..6608)
                     /locus_tag="O2458_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCZ3888045.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6610..7278)
                     /locus_tag="O2458_002196"
     CDS             complement(6610..7278)
                     /locus_tag="O2458_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ3888046.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(7740..8858)
                     /gene="mnmA"
                     /locus_tag="O2458_002197"
     CDS             complement(7740..8858)
                     /gene="mnmA"
                     /locus_tag="O2458_002197"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCZ3888047.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(8859..10001)
                     /locus_tag="O2458_002198"
     CDS             complement(8859..10001)
                     /locus_tag="O2458_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ3888048.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            10313..11326
                     /locus_tag="O2458_002199"
     CDS             10313..11326
                     /locus_tag="O2458_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ3888049.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT         3444 a         2205 c         1587 g         4101 t
ORIGIN      
        1 aattatttat acattgcttg acttaaaaaa tactcttgcc tccccatctt taaggttagc
       61 aagagtaaaa tctttttaat tattcttcca tttcagtatt tacaacttct tcaatcatgt
      121 cccattcttc atcagtttcg attggtacta acttaccacc gtcacctgac tcatctggtt
      181 cattgatcat tggtacaagc tcaatcatat cgtcttcatc tgattgagca ccttcttcag
      241 ctaagataac atactctttt ttgaattcag gatgataaaa ttctaaaact tttcggtata
      301 aaacttcatt tccctcttca tcgaataaag ttaataattc ttcttcgtta ttaatttcta
      361 gttgtgaatc atgattatgt tcagtcatag taaaatctcc ttttaatgta gtgaatctaa
      421 atagccttgt aaaataaata ccgctgccat tttatcaatc acttgttttc ttttttgtct
      481 tgaaacatct gcttctaata atgatcgttc agcagccatt gtgcttaatc tttcatccca
      541 catcacaatc tcaatagaag gataagcttc taataatttt tctttatatg ttaacgaagc
      601 ttcgcctcga aatcctattg aattattcat gtttttaggt agtcctatta cgactgtacc
      661 cacattatgt tttttaataa tgtctactaa ttggtcaata cctaattcat tattttcttc
      721 attgattcgg agtgtgtcta atccttgtgc cgtccaaccc attatatcac taattgcaat
      781 tcctaccgtt ctactaccga catcgagtcc taaaatttta tgttgtaaca taaattattt
      841 attttgctct tttaaatagt aagaaacaag ctcttccata ataacatctc tatcaatatg
      901 acgaatttga tttcttgctt cattttggcg tggaatatac gcagggtcac ctgataataa
      961 ataacctaca atttggttta cggcattata tcctcgttca tctaatgttc gataaacatt
     1021 atttaaaaca tctcttacat cttgcgttgg aagttcttca tagtcgaatt tcattgtttt
     1081 atcaaagttt tccatttgcg acactccttt aattacaaat ataactcact atcatcatac
     1141 aatattatgg ctttaaatta tagattttta atgtaatctt taataaagct taatgatttt
     1201 gagatatttt caggttgtgt accgccacct tgagccatat ctggacgacc gccaccttta
     1261 ccaccaacga ttggtgccat ttgtttgata agatcaccgg ctttaacgtt atttgttaaa
     1321 gatttaggga cagttgcaac catcgatact ttatcatcaa cattacttgc aagaatgata
     1381 attgtatctt gtagtttaga tttaaaatcg tccattgtcg agcgaattgc tttcgcattt
     1441 ggtacatcca cttcagtaac caatacttta tagccattga tttcttcaac ttgatcttca
     1501 atattaccca ttttaagtga tgtgatttct ttgtcacgtt gctctaattg ttttaataat
     1561 gctttttctt catcttgtaa ttgtgttaac ttatcgacta cttgatcatc agatttcact
     1621 ttcagctgtg atttcatcgt attaaatttc tcttgaatat cttctaaata taagaaagct
     1681 gctttacctg ttaatgcttc aatacgacgc acaccagctc ctgtacctga ctcacttact
     1741 attttgaata agccaatttc agaagtattg cggacatgaa taccaccaca taattcaatt
     1801 gaaaatggtg ccatatttac tacacgcaca acatcaccat atttttcacc gaataatgcc
     1861 attgcgccca tttctttagc tgaagcaata tccatttctt gaatgttaac gtcaatacct
     1921 ttccaaattt cttcatttac taagcgttca acttgatcaa tttcatcatt agtcattgga
     1981 ccaaaatgag agaaatcaaa acgtaaacga tctgcttcta ctagtgaacc agcttggtta
     2041 acatgatcac ccagtactga tttcaacgct gcatgtaata aatgtgttgc actatggttc
     2101 ttttgaatgt cacgtcgatc attttggttc acttcagcag acactgtagc gccaacattt
     2161 acttggccaa attgtactac tcctttatgc aagttttgac catttggtgc tttggttact
     2221 tcactaacag caatttcaaa attgtcatta taaacaatac ctgtatccgc aacttgtcca
     2281 ccactgattg cataaaatgg tgtttccgtt aacatgaagt atactgtttc acccgcttca
     2341 acttgtgaaa cttcttcacc attgtatatc aagtgtgtta gtgttgtttg agctgtcgca
     2401 gtatcataac caacaaaagt acttgcagat gtaatatttt tcaatacttc actttgaact
     2461 tgcattgatt gagaattttg acgtgcttga cgtgcacgat cacgttgttg ttgcatttct
     2521 gactcgaatg ttgtcatatc aactttcaat cctgcttgca ctgctatttc ttcagttaat
     2581 tcaattggga acccatacgt atcatacaat ttaaatgcat ctttcccatt aatttcattt
     2641 gttgtcgctt tagctttttt aattaattca tttaaaatcg ctaaaccatc ttctaatgtt
     2701 tcatggaatc gttcttcttc agactttata acacgcttaa tgaaatctgc tttttcctta
     2761 acatttggat aatatggttc cataatgtct gcaacaatat caacaagttt gtacataaat
     2821 ggctcattga ttcctaacgt ttgactaaaa cgaacggcac gacgtaacaa tcgacgtaat
     2881 acataccctc taccttcatt ggcaggtaat gcaccatcag aaattgcaaa tgcaatcgta
     2941 cgaatgtggt cagcaattac tttaaatgcc acatcttgtt cgttgtttac taaatattgt
     3001 ttacctgata ctttttcgat ttcattcatt ataggcataa ataaatctgt ttcatagtta
     3061 gtacgtacat tttgagaaac tgaggccata cgctcaagcc ccatgccagt atcaatattt
     3121 ttattaggta atggtgtgta actatgatct ttattatgat tgaattcact aaatactaag
     3181 ttccatactt caagatagcg ttcattttct ccacctggat acatttcttc tgccggatcg
     3241 tcttgtccat atgcttctcc gcgatcatag aaaatctcag tgttcggtcc tgaaggccct
     3301 tcaccaatat cccagaagtt accttcaatg cgaataatac gactttcttc aagcccaata
     3361 tctttatgcc aaatgttgta tgcttccata tcttccggat gaatcgtaac gtacaattta
     3421 tctggctcca tacccatcca tttatcactc gttaaaaatt cccaagcaaa ttcaatcgct
     3481 tcttgtttaa aataatcacc aattgagaag ttacctaaca tttcaaagaa tgtatggtga
     3541 cgcgctgtga aaccaacatt ttcaatatca tttgtacgaa tagctttttg agagtttaca
     3601 attcttggct ttttaggtgt ttcacgtcca tcaaaatatt tctttaatgt tgctacacct
     3661 gaattaatcc ataataatgt atcatcatca attggcacta atggtgcaga aggttcaacc
     3721 atatgtcctt tttcaacaaa gaaatctaga tatttttgtc taatttcact cgcttttaac
     3781 tttttcatca tttacacatc ctatttactg tttttaaatt accattccat aaaaattgat
     3841 gacacagata gtcgatttgc aaaactagta taaatcaata tcatttttta ttattaaaaa
     3901 ataaaaaacg cccatcctca aaagggacga acgttatcgc ggtaccaccc tagttataaa
     3961 tgcaattcaa cacatttatc actttaattc gactatacag ttgtgcataa agtagcgttc
     4021 actaatgttt gttgtacttt tcaccaacca gtacatctct gataaacaaa tcattaacta
     4081 ctcatcttta tacgaattta attctatttt agttacattt acgcttgttg tcaacgttct
     4141 ataaagtcat acggcgtgat ttctcccata ttaatcattg ggtcaatttt aaacattgta
     4201 gcttccgtta atacatttgt atctgttttt gttgaatcag acataacttc ttcactatca
     4261 ttcgatgaca ttggcgcttc tacttgatca tctattgtcg tttgtgaagc tcctgtatca
     4321 ttagttgctg tgttttccag catttcttca tcttctgaat taaaataatt tttcaacaat
     4381 gtacataatt gtgttaaacg cgcttgacca tttgttttca atccaatatc aaatgcttcc
     4441 ggatcaccaa gtaaaactaa actcgttttc gctctagtta aaccagtata taatatcggt
     4501 ctttgtaaca ttctaaaata ctgtttaaca ataggcatga taacaatagg aaattctgaa
     4561 ccttgtgatt tatggattga tgtacaataa gcatgtgtta attccatcat atcttgtttc
     4621 gtaaatgtaa tttcattacc ttcaaaatcc acaacaagta catctttatt aagggcattt
     4681 tctttcgccc aaaaaatacc aacaataact cctatgtcac cattgaatat gttatcattt
     4741 ggcctattaa caagttgtaa tactttgtca ccttttctaa agactacatc accaaactca
     4801 atttctcgtg tgtctttctt tttagggttt aaaatatctt gtaaaacttg atttaaacgt
     4861 ttaataccgg catttccttt atacattggt gcaagcactt gaatatcagc catagtatac
     4921 cctttattaa cagcactagt aactaccttc tcaacaactg ttggtatttg gtttgcctga
     4981 cagttaataa aacttctatc atgaaaacgc tgtgtaatat caattttctg acccaacttc
     5041 attcgatgtg ctaattctat aatgcttgaa ccatcttgtt gacgatatac ttcagtcaga
     5101 tttactcgtg gtatagcttt cgattcaatt aaatctttaa atacttgacc aggacctaca
     5161 gaaggcaatt ggtcctcatc acctacaaat atcaattgtg catctaaagg aactgcactt
     5221 aaaaattggt ggaacaacca agtatctacc atagacatct catcaatgat tatgagtcgt
     5281 gcgtttattt cattttctaa tatatcctct ggctttgtgt cttgattcca acctattaaa
     5341 cgatgaatcg tcattgcttc taatccagtt gactcttgta gtctcttaga cgctcttcct
     5401 gttggcgctg ctaatacaac tggataatca tcattgacat aatcatcata atctaatgat
     5461 aagccatgaa tctcagcata taattcaaca atacctttaa ttactgtcgt ttttcctgtt
     5521 cccggtccac cggttaatag catcacctta gaattgatag ccgtttgcaa agcttctttt
     5581 tgtgaagctg catagttcac ttgattcgca tcttctattt caccaatatg catttgtaaa
     5641 tctgactgtt caatttctgt aagtttattt gtatgcgtct ttattctgaa taagttttga
     5701 acacttttga tttcagaata atacaaactt ggaattgcaa cttgttcatt gtcaataatt
     5761 agtcgttttt cctcatttaa gtattgcaac atttcgtcta atttttcagg ttcgatgacc
     5821 tcttcatctt gataatttaa tacatcaacc gttaaatcta taacaacatt gataggcaaa
     5881 tatgtatgtc cctgtttaat acattcttct tctaacgtat agagcaacgc agcttttaat
     5941 cgttcattat cgttataagc gataccaata tttctagcaa gttgatctgc tttattaaaa
     6001 ccaatacctt taatatcata aatcaattga tatggatttc gatctaaaat agtcagtgta
     6061 tcgccgagat aaaactgata aattgccatt gaaagtttag gaccaaaccc taaatcatgt
     6121 aaacgaatca ttattttttc agattcttga tttgctgaaa tttgttctgc aatttgtttc
     6181 tgtttctttt tagataatcc cgaaactttt tctagcactg aatggtcatc taatatatca
     6241 tttatcgcat tgtcacctaa tgtattaaca atattttgag ctgtcttttt acctacacct
     6301 ttaaacaaat cactagataa ataacttata attgcttctt tcgtttgtgg catttctttt
     6361 tcaaaagtct ctgcttttaa ttgtttacca taacgtggat gatcaacaac ttgcccttta
     6421 aatgtgtaga catcgccttc aacaatattc ggaagaaacc ctacaacagt tggcattgta
     6481 tcaaagtctt catttgtttc aatagtatct actttaagca ctgtataaaa attatcactg
     6541 ttttgaaaca atatcgcttc aacagtacct ttgatcattg aataatcaaa tagtgtaggg
     6601 tctgacatgt tactcctcct ctttcatttt agtgaatgtt ttcagcgcat gctgacttaa
     6661 taagtgttta gggtcgatag tcacagcttc tttaaaatga gttattgctt catcaatatc
     6721 ttcatttttc ataaataacg ctaagcccaa attgtatctt gcatcaacat gatttttatc
     6781 aatcgttaat acatgtttaa gttgagttat ggcttcatta aacatttcta attgacataa
     6841 tacaagacca tattgaaatt gaacttctgc atctttgtct ttatctagtt ccgcagcagt
     6901 cattaaatac ggcaatgcca gcttaaatga ttctaactga ttaaacgcca taccgatcat
     6961 ataattacaa tcaacttgtt caatctctgt ttgtaatgct tgttgatata atttaatagc
     7021 ttcttgataa cgttgctgat tataatatac atttgctaga ttaaaaaata cgacgccatt
     7081 cttcggatct attgttaaag ctttttggaa aaaacgctct gccttttcaa tctcattcgc
     7141 atcagcaagt acgataccag cattaatata attttcaata attgtaggat tttcttcgat
     7201 atttccgaac aatgcttgta acgcttcttc tatttttcca ttttgtatgt attgataaat
     7261 tgtttgttga tctatcattt acgaacctca tttctcatca attataacat cttgataaat
     7321 tgtatgtctc gaatcactta acaacgaata aaatataatc taatatcatc ttcattcatg
     7381 aaaaagcggg aatggaatag aaatgcttaa gaaccattaa cggtttatta tgtaatggtt
     7441 cttccacatt agccaccact attatgtact taaaaataag aatacataat tagattcatg
     7501 catagggagt gggacagaaa tgatatttta acaaaattaa attcgttatc cccaactggc
     7561 attgcctgta gaatttcttt acgaaattct ctatgttgtg gtcccgccaa tataacattg
     7621 tagagcctag gacattgtga tgtcccagac tctatcctca tgaattattc tcatcaaaaa
     7681 ctgtctttcg tcattttcaa cgttgaaact tcaaataagt aatttattgt tgccattgtt
     7741 tatacaacat aatttaattg accttcattt ttgaacacat cgtcaattgt tgcaccacca
     7801 agacacacat caccttgata aaaaacaact gcttgtccag gtgtgattgc tcttactggc
     7861 tcagcaaaag taacacgtag tgcatggtcg ttttcacgtt tcacaaaaac tttcgtatct
     7921 ttttggcgat atctaaattt agctgtacat tcaaaacctt gatctaagtc attatcttct
     7981 ggatttacaa atgaatagtc tgaagcaatt aagtaatcac tgtataatgc atcgtgatgg
     8041 aatccttgtt ctacatataa aacattatct tttaggtttt taccgacaac aaaccaagga
     8101 tcgccatctc cacctatacc taatccatgt ctttgtccta ttgtgtaata catcaaacca
     8161 ctatgtttac ccattttctt accatcaagt gttatcatat cacccggttg tgcaggtaaa
     8221 tattgtgata aaaatgtttt aaagtttttt tcgccgataa aacaaatgcc tgtagaatct
     8281 tttttcttag cagtaacaag tccttgttct tcagcaattc gacgcacttc actcttttcg
     8341 atgtcgccaa ttgggaacat cacttttgaa agttgttgtt gagataattg attcaagaag
     8401 tatgtttgat ctttattatt atctacacca cgtaacattt caacatgacc atcttcatga
     8461 cgatgtatgc gtgcgtaatg tcctgttgct acataatctg cacctaaatt catcgcatga
     8521 tctaaaaagg ctttaaactt aatttcttta ttacacataa cgtctggatt tggagtacga
     8581 ccttttttgt attcatctaa gaaatacgta aagactttat cccaatattc tttttcaaaa
     8641 ttaacagcgt aatacggaat gccaatttga ttacacactt caataacatc gttgtaatct
     8701 tcagttgcag tacatacgcc attttcgtca gtgtcatccc agtttttcat aaatatgcca
     8761 atgacatcat aaccttgttc ttttaagacg tgggctgtta cagaactatc tacaccgcct
     8821 gacataccaa cgacaacacg tatatcttta tttgacaatt atgactcctc cttaaattta
     8881 aaatatattt tatgaatttc agctacaatt gcattaattt cattttcagt agtcaattcg
     8941 ttaaaactaa atcgaatcga atgatttgat cgctcctcat cttcgaacat tgcatctaaa
     9001 acatgcgacg gttgtgtaga gcctgctgta catgcagatc cagacgacac atagatttgt
     9061 gccatatcca acaatgttaa catcgtttca acttcaacaa acggaaaata tagatttaca
     9121 atatggcctg tagcatccgt cattgaacca tttaattcaa atggaatcgc tctttcttgt
     9181 aatttaacta aaaattgttc ttttaaattc attaaatgaa tattgttatc gtctcgattc
     9241 ttttctgcta attgtaatgc tttagccatc ccaacaattt gcgcaagatt ttcagtgcct
     9301 gcacggcgtt tcaattcttg ttcaccgcca agttgaggat aatctagtgt aacatggtct
     9361 ttaactagta atgcaccgac accttttggt ccgccaaact tatgagcagt aatactcatt
     9421 gcgtcgatct caaattcgtc aaacttaaca tcaagatgtc caattgcttg aaccgcatca
     9481 acatggaaat atgcatttgt ctcagcaata atatcttgaa tatcataaat ttgttgcact
     9541 gtgccaactt cattatttac aaacataata gatactaaaa tcgtcttatc tgtaattgtt
     9601 tcttcaagtt gatctaaatc aatagcacct gtatcatcaa catctagata tgttacatca
     9661 aaaccttctc gctctaattg ttcaaaaaca tgtaacacag aatgatgttc aatcttcgat
     9721 gtgataatgt gattacccaa ttgttcattt gcttttacta tgcctttaat tgccgtatta
     9781 ttcgattctg ttgcgccact cgtaaatata atttcatgtg tatctgcacc aagtaattgt
     9841 gcaatttgac gtcttgactc atctaaatat ttacgcgcat ctcttccctt agcatgtatt
     9901 gatgatggat taccataatg cgaattgtaa atcgtcatca tcgcatctac tacttcaggt
     9961 tttactggtg tggtcgcagc ataatctgca taaatttcca tgtttggaca ctcctcacaa
    10021 ttttatcaat gttccaataa tagcacctta catactattt ttctactttt ctgtttaact
    10081 ttatttataa tgtttttaat tatattttac cattttctac acatgctttt cgataggctt
    10141 ttttaagttt atcgctttat tcttgtcttt tttataaatt ttagtatttg cagatatttt
    10201 tttatttgta aaatgtaacg tactattatt ttggttatga gcaatttaat atttatctgg
    10261 ttattcgatt ggtatacttc ttatatcata aaaaaggaag gacgatataa aaatggcgat
    10321 taaatattca gcattaaacc ttgtccctat tcgagaaggt gaagatgaac aaacagcaat
    10381 taatgatatg gttaatctcg cacaacattt agacgaatta tcatatgaaa gatattggat
    10441 tgctgaacac cataacgctc ccaacctagt aagttcagca actgctttat taattcaaca
    10501 tacgttagaa catacgaaac acatacgtgt aggttctgga ggcatcatgt tacctaatca
    10561 tgctccatta atcgttgcgg aacaatttgg cacgatggca acattatttc caaatcgtgt
    10621 cgatttagga ttaggacgtg cacctggaac agatatgatg accgcaagtg cattaagacg
    10681 agatcaacat gatggtgttt ataaatttcc agaagaggtt tcattattac aacaatattt
    10741 cggccctgct caccaacaag catatgttcg tgcttatcca gcagtaggta aaaatgtgcc
    10801 tttatacatt cttggttctt caacagattc tgcacattta gctgctcgca aagggcttcc
    10861 atatgtgttc gctggacatt ttgcacctca acaaatgaaa gaagctatcg aaatttacaa
    10921 aacgttattt gaaccttctg atgtattaga cgaaccttat gttattgtat gtttaaatac
    10981 aatcgttgct gaaaatgatg acgaagcaca atatttagct tcatctatgg cacaagtaat
    11041 ggttagtatc actcgtggca gaatgcagcc cgttcaaccg ccaacacatg aactacaaaa
    11101 tatattaacg ccgagagaat acgcgatggc tatggaaaga cagaaaatat cattaatagg
    11161 ttcagaaaat actgttcaac aaaaaattca agattttatg gaaacttatg gtgaagtcaa
    11221 cgaaattatg gcaataagtt atatttatga taaagatatg caattagact cttatcgtcg
    11281 gttcaagaat gttataaatc agataaatga aaaaaacact ttataatgtg ataaata
//
LOCUS       DALAAR010000076        11292 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000076 DALAAR010000000
VERSION     DALAAR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.078"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            361..2049
                     /gene="ilvD"
                     /locus_tag="O2458_002200"
     CDS             361..2049
                     /gene="ilvD"
                     /locus_tag="O2458_002200"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCZ3888050.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            2077..3846
                     /gene="ilvB"
                     /locus_tag="O2458_002201"
     CDS             2077..3846
                     /gene="ilvB"
                     /locus_tag="O2458_002201"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCZ3888051.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3846..4100
                     /locus_tag="O2458_002202"
     CDS             3846..4100
                     /locus_tag="O2458_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ3888052.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4237..5241
                     /gene="ilvC"
                     /locus_tag="O2458_002203"
     CDS             4237..5241
                     /gene="ilvC"
                     /locus_tag="O2458_002203"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCZ3888053.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5271..6800
                     /locus_tag="O2458_002204"
     CDS             5271..6800
                     /locus_tag="O2458_002204"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ3888054.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6803..7849
                     /gene="leuB"
                     /locus_tag="O2458_002205"
     CDS             6803..7849
                     /gene="leuB"
                     /locus_tag="O2458_002205"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCZ3888055.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7863..9233
                     /gene="leuC"
                     /locus_tag="O2458_002206"
     CDS             7863..9233
                     /gene="leuC"
                     /locus_tag="O2458_002206"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCZ3888056.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9234..9806
                     /gene="leuD"
                     /locus_tag="O2458_002207"
     CDS             9234..9806
                     /gene="leuD"
                     /locus_tag="O2458_002207"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCZ3888057.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9821..11089
                     /gene="ilvA"
                     /locus_tag="O2458_002208"
     CDS             9821..11089
                     /gene="ilvA"
                     /locus_tag="O2458_002208"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCZ3888058.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3820 a         1858 c         2177 g         3437 t
ORIGIN      
        1 aagaaaattt aaaatatttg ttgacatact aaagcagata tagtaaatta aatttatcaa
       61 atttttagac aattctaact attaaagtga tatataccat tcacggaagg agtataataa
      121 aatgcttaat caatatactg aacatcaacc gacaacttca aatattatta ttttattata
      181 ctctttagga ctcgaacgtt agtaaatatt tactaaacgc tttaagtcct atttctgttt
      241 gaatgggact tgtaaacgtc ccaataatat tgggacgttt ttttatgttt tatctttcaa
      301 ttacttattt ttattactat aaaacatgat taatcattaa aatttacggg ggaatttact
      361 atgcgaagcg acatgatcaa aaaaggagat caccaagcac cagcaagaag tcttttacat
      421 gccacgggcg cgctaaaaag tccaactgat atgaacaaac catttgtagc tatttgtaac
      481 tcttatattg atattgttcc tggacatgtt cacttgagag agcttgcaga tatagctaaa
      541 gaagcaatta gagaagccgg tgccattcca tttgaattca atacaattgg tgttgatgat
      601 ggaatagcta tgggacatat cggaatgcga tattctctac catcacgtga aattattgca
      661 gatgcagctg aaactgtaat taacgctcat tggtttgacg gcgtatttta cattcctaat
      721 tgtgacaaga ttacacccgg tatgatttta gcagccatga ggacaaacgt accagctatc
      781 ttttgctctg gtggaccaat gaaagctggc ttatctgcac atggaaaagc attaacactt
      841 tcatcaatgt ttgaagcagt cggcgcattt aaagaaggat cgatttctaa agaagaattt
      901 ttagatatgg aacaaaatgc ctgccctact tgtggttcat gtgctgggat gtttactgca
      961 aattcaatga actgtttgat ggaagtttta ggtctaacat taccatacaa cggtactgca
     1021 cttgcagtca gtgatcagcg acgagaaatg attcgccaag cagcttttaa attagttgaa
     1081 aatattaaaa atgatttaaa accacgtgat atcgttactc gcgaagcaat tgatgatgca
     1141 tttgcacttg atatggctat gggtggttca acaaacacgg tactgcatac gttagccatt
     1201 gccaatgaag ctggtattga ttatgactta gagcgcatta atgctattgc caaacgcacg
     1261 ccatatttat caaaaatagc acctagttca tcgtattcaa tgcatgatgt gcatgaagct
     1321 ggtggcgtcc cagcaattat taatgaattg atgaagaaag atggcacgtt acacccagat
     1381 agaatcacag ttactggcaa aacgttacgt gaaaataacg aaggcaaaga aattaagaac
     1441 tttgatgtca ttcaccctct tgatgcacca tatgatgcac aaggcggttt atctatctta
     1501 tttggtaata tcgcccctaa aggcgcagtt attaaagttg gcggcgttga tccatctatc
     1561 aaaacattta ctgggaaagc aatttgtttc aattcgcatg atgaagctgt tgaagcaata
     1621 gacaatcgta ccgttcgtgc aggccacgtc gttgtcatta gatatgaagg acctaaaggt
     1681 ggaccaggta tgcctgaaat gttagcacct acttcctcta ttgttggtcg cggcttaggt
     1741 aaagatgttg cattaattac tgatgggcgt ttttccggtg ccacaagagg tattgcagtt
     1801 ggtcatattt cccctgaagc tgcatctggt ggaccaattg ccttaattga agatggtgat
     1861 gagattacta ttgatttaac aaatcgtaca ttaaacgtaa accagcctga agatgttcta
     1921 gcgcgtcgcc gagaatcttt aacaccattt aaagcgaaag taaaaacagg ttatctagct
     1981 cgttatactg ccctagtaac tagcgcaaat acaggtggcg tcatgcaagt ccctgagaat
     2041 ttaatttaat ttatttttat attggagatg gttaaaatgt ctaaaactca acatgaagta
     2101 aaccaaaata ttgacccttt aaaaatggct gaatcacttg aacctgaaca actaaatgaa
     2161 aaaactttaa atgatatgcg ttcaggatca gaagtgctag tagaagctct acttaaagaa
     2221 aatgtggatt atttattcgg ttatcctggt ggtgccgtac tacctttata tgacacgttt
     2281 tatgatggta aaatcaaaca tattttagca agacacgaac aaggtgctgt tcatgctgca
     2341 gaaggttatg cacgtgtatc tggtaaaact ggcgtcgttg tagttacaag cggtccaggt
     2401 gcaactaatg taatgacagg tattacggat gcacattgcg actctttacc tctagttgta
     2461 ttcactggac aagttgctac accaggcatt ggtaaagatg cattccaaga agcggatatt
     2521 ctatctatga cttcaccaat tacaaaacaa aattatcaag tgaaacgtgt tgaagatatc
     2581 cctaaaatcg tacacgaagc tttccatgta gctaattctg gacgcaaagg tcctgtagtg
     2641 attgattttc caaaagatat gggtgtttta gctacaaatg tggatttatg cgacgaaatc
     2701 aatattccag gttatgaagt tgttacagaa ccagaaaata aagacattga cactttcatc
     2761 tcacttttaa aagaagcgaa aaagcctgtc gtattagccg gcgcaggtat taatcaatca
     2821 aaatcaaatc aattattaac acagtttgtt aataaacatc aaatcccaac agttactact
     2881 ttactaggtc taggtgctgt accttatgag gatacactat ttttaggtat gggaggaatg
     2941 catggttctt atgctagtaa catggcatta actgagtgtg atttactcat taatttaggt
     3001 agccgcttcg atgatagatt agcaagcaaa cctgatgcct ttgcacctaa cgccaaaatt
     3061 gtacatgtag atattgatcc ttcagaaatc aataaagtta ttcatgtaga tttaggtatt
     3121 attgcagact gtaaaagatt tttagaatgt ttaaatgata aaaatgttga gactatagaa
     3181 cacagtgact gggttaaaca ttgtcaaaat aataagcaga aacacccatt taaacttggt
     3241 gaagaagatc aagtattttg taagccacaa caaacaatcg aatatatcgg caaaattaca
     3301 aatggtgaag caattgttac tacagacgtg ggacaacatc aaatgtgggc agctcaattt
     3361 tatccattta aaaatcacgg acaatgggtt acaagcggtg gtttaggaac aatgggattc
     3421 ggtattcctt cgtcaattgg tgccaaatta gctaatcctg ataaaacagt cgtatgtttc
     3481 gtcggtgacg gtggtttcca aatgacaaac caagaaatgg cacttttacc cgaatatggt
     3541 ttagatgtca aaatcgtact aatcaataat ggaacattag gtatggttaa acaatggcaa
     3601 gataagttct ttaatcaacg cttctcacac tcagtattta atggtcaacc tgattttatg
     3661 aaaatggcag aagcatatgg cgtcaaaggt ttcttaatcg ataagccaga acaactggaa
     3721 gaacaattag atgcagcgtt tgcttatcaa ggaccagctt taattgaggt tcgtatttcc
     3781 cctactgaag ctgtaacccc aatggttccg agtggcaaat caaatcatga aatggagggc
     3841 ttataatgac aagaattctt aaattacaag ttgcggatca agtcagcacg ctaaatcgaa
     3901 ttacaagtgc ttttgttcgc ctacaatata atatcgatac attacatgtt acacattctg
     3961 aacaacctgg gatttctaac atggaaattc aagtcgatat tcaagatgat acatcacttc
     4021 atatattaat taaaaaatta aaacaacaaa ttaatgtttt aacggttgaa tgctacgacc
     4081 ttgttgataa cgaagcttaa ttttaagaca aaggcaatga tgcgctaatt agttatagat
     4141 atatcatagg ctgctagtta acatctgcca ctattacaaa gttatatttc agaattttcg
     4201 aaacacaaaa tatttaatta tttggaggaa tttattatga caacagttta ttatgatcaa
     4261 gatgtaaaaa cggacgcttt acaaggcaaa aaaattgcag tagtaggtta tggatcacaa
     4321 ggtcacgcgc atgcacaaaa cttaaaagac aatggatatg atgtagtcat cggcattcgc
     4381 ccaggtcgtt cttttgacaa agctaaagaa gatggatttg atgtgttccc tgttgcagaa
     4441 gcagttaagc aagctgatgt aattatggtg ctattacctg atgaaattca aggtgatgta
     4501 tacaaaaacg aaattgaacc aaatttagaa aaacataatg cgcttgcatt tgctcatggc
     4561 tttaacattc attttggtgt tattcaacca ccagctgatg ttgatgtatt tttagtagct
     4621 cctaaaggac cgggtcattt agttagacgt acatttgttg aaggttctgc tgtaccatca
     4681 ctatttggta ttcaacaagg cgcttcaggt caagcacgta atattgcttt aagttatgca
     4741 aaaggtattg gtgcaactcg tgcaggtgtt attgaaacaa catttaaaga agaaactgag
     4801 acagatttat ttggtgaaca agcagtactt tgcggtggtg tatcgaaatt aattcaaagt
     4861 ggctttgaaa cattagtaga agcgggttat caaccagaat tagcttattt tgaagtatta
     4921 catgaaatga aattaatcgt tgatttgatg tatgaaggcg gtatggaaaa tgtacgttac
     4981 tcaatttcaa atactgctga atttggtgac tatgtttcag gaccacgtgt tatcacacca
     5041 gatgttaaag aaaatatgaa agctgtatta actgatatcc aaaatggtaa cttcagtaat
     5101 cgctttatcg aagacaataa aaatggattc aaagaatttt ataaattacg cgaagaacaa
     5161 catggtcatc aaattgaaaa agttggtcgt gaattacgcg aaatgatgcc ttttattaaa
     5221 tctaaaagca ttgaaaaata agatagacct acaatgagga gttgttaaat atgagtagtc
     5281 atattcaaat ttttgatacg acactaagag acggtgaaca aacaccagga gtgaatttta
     5341 cttttgatga acgcttgcgt attgcattgc aattagaaaa atggggtgta gatgttattg
     5401 aagctggatt tcctgcttca agtacaggta gctttaaatc tgttcaagca attgcacaaa
     5461 cattaacaac aacggctgta tgtggtttag ctagatgtaa aaaatctgac atcgatgctg
     5521 tatatgaagc aacaaaagat gcagcgaagc cggtcgtgca tgtttttata gcaacatcac
     5581 ctattcatct tgaacataaa cttaaaatgt ctcaagaaga cgttttagca tctattaaag
     5641 aacatgtcac atacgcgaaa caattatttg acgttgttca attttcacct gaagatgcaa
     5701 cgcgtactga attaccattc ttagtgaaat gtgtacaaac tgccgttgac gctggagcta
     5761 cagttattaa tattcctgat acagtcggct acagttacca tgatgaatat gcacatattt
     5821 tcaaaacctt aacagaatct gtaacatctt caaatgaaat tatttatagt gctcattgcc
     5881 atgacgattt aggaatggct gtttcaaata gtttagctgc aattgaaggc ggtgcgagac
     5941 gaattgaagg cactgtaaat ggtattggtg aacgagcagg taatgcagca cttgaagaag
     6001 tcgcgcttgc actatacgtt cgaaatgatc attatggtgc tcaaactgct cttaatctcg
     6061 aagaaactaa aaaaacatcg gatttaattt caagatatgc aggtattcga gtgcctagaa
     6121 ataaagcaat tgttggccaa aatgcattta gtcatgaatc aggtattcac caagatggcg
     6181 tattaaaaca tcgtgaaaca tatgaaatta tgacacctca acttgttggt gtaagcacga
     6241 ctgaacttcc attaggaaaa ttatctggta aacacgcctt ctcagagaag ttaaaagcat
     6301 taggttatga cattgataaa gaagcgcaaa tagatttatt taaacaattc aaggccattg
     6361 cggacaaaaa gaaatctgtt tcagatagag atattcatgc gattattcaa ggttctgagc
     6421 atgagcatca agcactttat aaattggaaa cactgcaact acaatatgtc tctagcggcc
     6481 ttcaaagtgc tgttgttgtt gttaaagata aagagggtca tatttaccag gattcaagta
     6541 ttggtactgg ttcaatcgta gcaatttaca atgcagttga tcgtattttc cagaaagaaa
     6601 cagaattaat tgattatcgt attaattctg tcactgaagg tactgatgcc caagcagaag
     6661 tacatgtaaa tttattgatt gaaggtaaga ctgtcaatgg ctttggtatt gatcatgata
     6721 ttttacaagc ctcttgtaaa gcatacgtag aagcacatgc taaatttgca gctgaaaatg
     6781 ttgagaaggt aggtaattaa ttatgactta taacattgtt gccctacctg gtgatggaat
     6841 cggtccagaa attttgaacg gatctctatc attgcttgaa attataagta ataaatataa
     6901 ctttaattat caaatagagc accacgaatt tggtggtgcc tctattgata cattcggcga
     6961 gcctttaact gagaaaacct taaatgcgtg taaaagagca gatgctattt tactgggtgc
     7021 aatcggtgga cctaaatgga cagatcctaa caatcgacca gaacaaggat tattaaaatt
     7081 gcgtaaatcc ttaaatttat ttgtaaatat acgccccact accgttgtca aaggcgctag
     7141 ttctttatca cctttaaagg aagaacgcgt tgaaggcaca gatttagtta tagtccgtga
     7201 attgacaagt ggtatttatt ttggagaacc tagacatttt aataatcacg aggccttaga
     7261 ttctcttact tatacaagag aagaaataga acgcattgtt cacgtagcat ttaaattggc
     7321 cgcttcaaga cgaggaaaac taacatcagt tgataaagaa aatgtattag cttctagtaa
     7381 attgtggcgc aaagtcgtaa atgaagtaag tcaattatat ccagaagtaa cagtaaatca
     7441 cttacttgtt gatgcttgta gtatgcattt aatcacaaat ccaaaacaat ttgacgtcat
     7501 cgtatgtgaa aacttatttg gcgatatttt aagtgatgaa gcttcagtga ttcctggttc
     7561 acttggttta tcaccttctg ctagttttag taacgatggt ccaagattgt atgagcctat
     7621 tcatggatca gcaccagata ttgcaggtaa aaacgttgcc aatccatttg gaatgattct
     7681 atctttagcg atgtgtttac gtgaaagctt aaatcaacca gatgctgcag atgaattaga
     7741 acaacatatt tatagcatga ttgaacatgg gcaaacgaca gcagatttag gcggcaaatt
     7801 gaatactact gatattttcg aaattctatc tcaaaaattg aatcactaag ggggagatgt
     7861 aaatgggtca aacattattt gacaaggtgt ggaacagaca tgtgttatac gggaaattgg
     7921 gcgaaccgca actattatac attgatttac accttataca tgaagttact tctcctcaag
     7981 catttgaagg acttaggctt caaaacagaa aattaagacg cccagattta acatttgcaa
     8041 cactcgatca caatgttcct actattgata tattcaatat taaagatgaa attgcaaaca
     8101 aacaaatcac aacattacaa aaaaacgcca tagattttgg ggtgcatatt tttgatatgg
     8161 gttctgatga acaaggtatt gttcacatgg taggacctga gacaggactt acacagcctg
     8221 gcaagacaat cgtttgtggt gactctcaca cagcaacaca tggagcattt ggtgctattg
     8281 catttggaat tggaacaagt gaagttgaac atgttttcgc aactcaaacg ctatggcaaa
     8341 caaaacccaa aaacttaaaa atcgatatta atggtacctt accaacaggc gtctatgcta
     8401 aggacattat tctgcattta attaaaacgt atggtgttga ctttggtaca ggctatgctt
     8461 tggaatttac tggcgaaaca attaaaaacc tttcaatgga tggtcgaatg actatttgta
     8521 acatggctat cgaaggtggt gccaaatacg gcataatcca acctgatgat ataacatttg
     8581 aatatgttaa agggagacca tttgccgata acttcgctaa atcagttgat aagtggcgtg
     8641 agctatattc tgatgacgac gcgatatttg atcgtgtaat tgaacttgat gtttcaacat
     8701 tagaaccaca agtgacatgg ggaactaatc ctgaaatggg tgttaatttc agtgaaccat
     8761 tccctgaaat caatgatatc aacgatcaac gtgcgtatga ttatatgggg ttagaaccag
     8821 gtcaaaaagc tgaagacatc gacttagggt atgtttttct cggttcatgt acaaatgcta
     8881 gactatcaga tttgattgaa gctagtcata ttgttaaagg aaataaagtt catccaaata
     8941 ttacagctat tgtcgtacca ggttctcgta cagtaaaaaa agaagcagaa aaattaggtc
     9001 tagatactat ctttaaaaat gcaggatttg aatggcgtga accaggatgt tcaatgtgtt
     9061 taggcatgaa tcctgaccaa gtacctgagg gcgtacattg tgcatctaca agtaatcgaa
     9121 actttgaagg acgacaaggc aaaggtgcaa gaacacattt agtatcccct gctatggcag
     9181 cagcagcagc tattcatggt aaatttgtgg acgtaagaaa ggtggttgtt taaatggcag
     9241 caatcaaacc tattacaaca tataaaggta aaatagtccc tctcttcaac gacaatatcg
     9301 atacagacca aatcattcct aaggtacact taaagcgtat ttcaaaaagt ggctttggtc
     9361 catttgcttt tgatgaatgg cggtacttac ctgatggttc agataatcct gatttcaatc
     9421 ctaacaaacc acaatataaa ggggcttcta ttttaattac tggagataat tttggatgtg
     9481 gttcaagtcg tgaacatgct gcttgggctc ttaaggacta tggttttcat attattattg
     9541 caggaagttt cagtgacata ttttatatga attgcactaa aaatgcgatg ttgcctatcg
     9601 ttttagaaaa aagtgcccgt gaacatcttg cacaatatgt tgaaattgag gtcgatttac
     9661 caaatcaaac tgtgtcatca ccagacaagc gtttccattt tgaaattgat gaaacttgga
     9721 agaataaact tgtaaatggc ttagatgaca ttgcaatcac cctacaatat gaatcattaa
     9781 tagaaaaata tgaaaaatca ctttaaggga gttgaatatt atgacagtca aaacaacagt
     9841 ttctacgaaa gatatcgatg aggcattttt aagacttaaa gatattgtca aagaaacacc
     9901 tttacaatta gaccattact tatctcaaaa gtatgattgt aaagtctatt taaaacgaga
     9961 agatttacaa tgggtacgtt cttttaaatt aagaggtgct tacaacgcta tttctgtttt
    10021 atcagatgaa gctaaaagta aaggtattac atgtgcaagt gcaggtaatc atgctcaagg
    10081 tgttgcctat acagctaaaa aacttaattt aaacgctgtt atctttatgc cagtcactac
    10141 acctttacaa aaggtaaatc aagtaaagtt ctttggaaat agtaacgttg aagttgtact
    10201 cactggtgat acatttgatc actgtttagc tgaagcttta acttatacaa gtgaacatca
    10261 aatgaacttt atagatccat tcaataatgt tcatacaatt tctggacaag gtacgcttgc
    10321 taaagaaatg ctagaacaag caaagtctga caatgttaac tttgattatc tatttgccgc
    10381 aattggtggt ggcggtttaa tttcaggtat tagtacttac tttaaaacct attcacctac
    10441 cacgaaaatt ataggtgttg aaccttcagg tgcaagtagt atgtatgaat ctgttgtggt
    10501 aaataatcag gtagtcacat tgcctaatat cgataaattt gtggacggtg catctgtagc
    10561 tagagttggc gatattacat ttgaaattgc aaaagaaaat gtagatgatt acgttcaagt
    10621 agatgaaggt gcagtttgtt ctacgatttt agatatgtat tcaaaacaag caattgtagc
    10681 agaacctgct ggcgcattaa gtgtaagtgc gcttgaaaac tataaagatc atattaaagg
    10741 taaaacagtg gtttgtgtca ttagtggtgg taataatgat attaatcgaa tgaaagaaat
    10801 tgaagaacgt tcattactat acgaagaaat gaagcattac tttatcttaa atttccctca
    10861 acgtccaggt gcattgagag aatttgtaaa tgacgtatta ggacctcaag acgatattac
    10921 taaatttgaa tacttaaaaa aatcttctca aaatacaggt actgtcatta ttggtattca
    10981 acttaaagat catgatgatt taatacaact caaacaacgt gtaaatcatt tcgatccttc
    11041 caatatttat attaatgaaa ataagatgtt atattcattg ttaatttaac acatagtaag
    11101 aaaaacagtc ataaattgat ttctaattga aatcatctta tgactgcttt ttattatact
    11161 ttacatttct cgtttcgtca gattcaaacg ttttcacttc gccaagccat ctttctttgt
    11221 gtttgctttt attttgacgt tttagacata aaaaaagaga ccttgcggtc tcaatgcggc
    11281 tcatcgcatc ca
//
LOCUS       DALAAR010000077        10963 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000077 DALAAR010000000
VERSION     DALAAR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.079"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..557
                     /locus_tag="O2458_002209"
     CDS             <1..557
                     /locus_tag="O2458_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ3888059.1"
                     /translation="YDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILIN
                     IPIKSENNKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKY
                     ASSKSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLE
                     HYTLDITKKDGKYYVKNMTNSIGG"
     gene            562..822
                     /locus_tag="O2458_002210"
     CDS             562..822
                     /locus_tag="O2458_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888060.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            834..1217
                     /locus_tag="O2458_002211"
     CDS             834..1217
                     /locus_tag="O2458_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ3888061.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1252..3747
                     /locus_tag="O2458_002212"
     CDS             1252..3747
                     /locus_tag="O2458_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ3888062.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            3801..5159
                     /locus_tag="O2458_002213"
     CDS             3801..5159
                     /locus_tag="O2458_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCZ3888063.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            5164..7011
                     /locus_tag="O2458_002214"
     CDS             5164..7011
                     /locus_tag="O2458_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888064.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            7001..8047
                     /locus_tag="O2458_002215"
     CDS             7001..8047
                     /locus_tag="O2458_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ3888065.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            8054..8644
                     /locus_tag="O2458_002216"
     CDS             8054..8644
                     /locus_tag="O2458_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888066.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            8700..9056
                     /locus_tag="O2458_002217"
     CDS             8700..9056
                     /locus_tag="O2458_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCZ3888067.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            9165..9668
                     /locus_tag="O2458_002218"
     CDS             9165..9668
                     /locus_tag="O2458_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ3888068.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            9649..10185
                     /locus_tag="O2458_002219"
     CDS             9649..10185
                     /locus_tag="O2458_002219"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCZ3888069.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            10405..10953
                     /locus_tag="O2458_002220"
                     /pseudo
     CDS             10405..10953
                     /locus_tag="O2458_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         3985 a         1218 c         2077 g         3683 t
ORIGIN      
        1 attatgatgt gaatattact gaaaagaaag aagttaaagt taagaagaaa aagaaaagtg
       61 aaaaagataa agatgagtat gaaacaaaaa cagaagaaaa gcaacgtaaa gtaaatcaaa
      121 acattttaat aaatatacct attaagagtg aaaataataa gtatgttgtt gttgaatatc
      181 cttattttac gccaatccct gatagtcaat tgaatcaagc taaaatggtt gaggataatt
      241 tgaaagacaa taaacgggaa gacaatccga aagcaaaagc ttttattgaa gatttcttta
      301 ataaatatgc ttctagtaag tctgatgata tggcctattt aatggataac cctgagggct
      361 tagaaggaac tagagaagtt tctcagataa gagaaattag gttatatcca aaaggcgatg
      421 attatgttgc taaagttgaa attttgatga aagataaaga ttctcctttg gagaatttgg
      481 agcattacac attagatata acaaaaaaag atggtaaata ttacgttaaa aatatgacta
      541 attctattgg aggttaatgt tatgagtggt ttatttaatt tttttgtttt aggtgctgac
      601 cgtccatcac ttggtggagt tggtgactgg ataagtaatg aagtaggtac gggtattggt
      661 ttagtagtat tggttgttgg tattgtaaaa tgggctagtg gtaaatatgg gcatatggtt
      721 atattatttg ttgttggtgg ctttttattc ttagtttcta aaggtcctga acaggtattt
      781 aatgcgattg caggtgtttg gaaaatgatt tttggcggtt gaggtgattc catttggata
      841 aaaaagctta taatttaaag cgaacttttg aacaaccgat agttatgtat gaatttacag
      901 ataagtttcg tattaataag ggttttcgtt tagatttttg ggctacattt ttgattgtgt
      961 ggtttatatt atttttgtta ttttggtatt tattacagcc tgtaattatg tcgattggtg
     1021 gacttatgtt tatttacttt acggttgcac cttattatat aacgaaatat attgttaagt
     1081 taaaacaaga tggaaagaaa ttatttttct tcttatggga ttttgtaata tttgttttta
     1141 atgtgcagtt aagaaaagtt aaattaagtt atgatgaaga agtagaatat catgataaaa
     1201 aaataacatt taaatagccc ctattggggt tatttttatt ggagggatga catgaattta
     1261 aaagcaccgt atgcaggttt aagagataat ttgttattaa cgaaaactgg cgaagtttgg
     1321 gcgtattatc gtgttcgttc tgagtctata tcaacagcaa attatgatgc aaaagaagaa
     1381 tcaaaaaagc gtatgcgtta ttttttagag tcgattaaag gttatcaaga ttttcatttt
     1441 gaaatgtatg accacgattt agatttgcga aataaattta aagaaattag taaagatttt
     1501 gatgatgaag cttttaatgt agctgaatat tattcaaaag aaactattga cgtattagaa
     1561 caagaattac aaatgattac gcataattct tttgttatgg gtgttaaaat acgtgaatta
     1621 gctgacgatg cagttacact taaagagatt ttaaaaagta cattatcaga tacggctgag
     1681 cgtattgttt cgaattttgg ttttgatgtg aatttagacg ataaaatttt agataagtat
     1741 aaaacatttg aacgagattt ggctgatagt tttctaggta ttggaggcga gcgtttaact
     1801 gagaatgaat tggcttatat tattagaaag ccttttattt caaatgcaac acatgacgta
     1861 caggaagaat cttcaagaag agctattaca gatatttata atgcagtaat tgaccctact
     1921 cgcttgagtg ttttagaagt tgaaaatgat aacgagaaat tttatacaac aacagttgtt
     1981 gttgatgatt ttccgttaga tatggaatat acacatttat ttgagaaagc tcaaaatatg
     2041 ccttttccgg ttgaatgcca tgtaaaagca aaaatcatta ataatgagaa agttaagaaa
     2101 aaatttgacc gtgcgaaaat aaatcataaa caacaagcaa aagaaaagca agatactggc
     2161 gacagtgctt cggaagatgt tcaagataat ttgtttgtat tgaatcaaat ggaacgagaa
     2221 gtgactggct caggtgtatt tattgagtgg gtatttatgt ttgtaattaa aagtgatgat
     2281 tatagagatt gtaaacgtaa agcaaagcgt ttgatgaaac gattaaagga aactcaaata
     2341 tatgcagtta atccattagc tgaccagtta caattgtttt atcaatgttt acatggtaat
     2401 gatttattta ttaataagta ttggttacaa agaacaacgg caacaggtat agcagagaat
     2461 ttatttggtg tgtctcaaag tttgggaaca aaaacgggtt tttatatagg gcgtattgat
     2521 aaatttatac gttctgtttc acgtgaggaa gctgttgcaa gttcaaggga tataattttg
     2581 tttagtttat tgttggccgc aaaaggtatt aagggggctg tcagtgatag tccgcatgtt
     2641 ttaattactg gtcaaactgg gaaaggtaaa tcatttttag caaaattatt atggatttat
     2701 acatcatttt ttaaaggtca aatgttgtac tgggatccaa aatctgagtt tgcagaatgg
     2761 tttgacagag taacagaaag taaggaaatg caagaaaagt atcccctatt tattaatcat
     2821 ttaaaaacat ttaaatatgt aacattgaat tatgaagatt caaccaatta tggttgtcta
     2881 gaccctattg tatttttaga tggtattgaa gctaatgaga tgttgaaatc agtgtttgat
     2941 gaaattaaat cttttgaaaa ttatcatcat attgaaacag caatttatca agctatttct
     3001 gatacggttg aagaacgtga aaatggtaaa aaagttggtt cattaaatgt aattgaaaaa
     3061 ttacagaata atgaagatga gcatgtaaaa aatgcaggcg atttattatt tgaaaaaacg
     3121 caaaacaata ttttaaaact tgtttttagt gatggtacga atccagcttt aaatattcaa
     3181 gaaaaagcaa ctattttaca ggttaaaggt ttagatatgc ctaaagcaga tgatgataca
     3241 tcaagttatt caacaagtga gaaaaatggg attacattaa tgttattaat tggtaagttt
     3301 ttagagaaat tcggttctcg tagagatgtt caaacgacta tatttattga tgagggttgg
     3361 gcttttagtg cttcaagaca aggtaaaaaa gttggtaagc gtataaaaag aacgggacgt
     3421 tctgagaata attcacttgt atttattaca caatcggtaa aagataaagc agatgatgat
     3481 ggtggtaact ttggttgtca ttttgcattt gatgaaaaag atgaacgtga ggatatttta
     3541 aaatctttag gcttagaata ttcaaaagaa tctcctgaaa acatggagat gttgaaagat
     3601 ttgaagaaag gtcaatgtat attcagtgat ttttatggac gtgttggaaa aatggttgta
     3661 cattgtccat ttgaggaaat gactgaagca tttagaacac aagaagattc tgcaagctct
     3721 aaagctgaag aaaaatttgc gatgtaattt ttagcacata caaaattgta tgtgcttttt
     3781 attttaaagg agtggatttg atggcaatga atgaaattgc tttatttaaa ggcgtacgta
     3841 tacagcctta tcagaagtat attgatttta tgtttgcaag tgtattagca tttatttttt
     3901 tggtatatcg tatatatatt ttgtttattg attataaatc agttaataaa aaattggata
     3961 tattgacgtt gtttgcatat tttaaaccgc atttattgta tttattaatt ggtacagcaa
     4021 ttatatttgt gatatgtaag cttattgctc agtttttaat acgttttaaa acaaaagatt
     4081 tggctgaaat gattgaaaca caaggttttc ataatcgtac agtaatcaag aaaacaactg
     4141 agtatatccc tattgattat aaacaaactg aatatgatta ttatccaaca atgttttata
     4201 agcgtggtaa aaaatctttt gttattcata ttaagaaaga cggttcgagg tttcaagata
     4261 attatttaga gttagagacg attattgaac caatgtttaa ttctgaatta gtagaaaaaa
     4321 agcatattgg tcgttatttg agatatgaat ttatgccact taaatataaa aagcgtattg
     4381 ttatgaatgg cgaacaagaa tcaaatacaa ttttttatga tacaaaaatt gctattacgc
     4441 atcaaattac ttgggatttt gtgaaagcac ctcatggatt ggttacaggt ataaccggag
     4501 gtggtaaaac atatttttta ttttatgtga ttcgtgagtt attccgtaga cattcagaag
     4561 tacgtttatt agacccaaaa gtttcagatt tgagctttat gaagcgcgtt attggtgatg
     4621 ataaagttgc agatactaaa ggtcaaatat taaaacaatt gagagaagct aataatgaaa
     4681 tggaagagcg ttttagatta atgaatgata gttcggatta taaaatcggg aatgatttcc
     4741 gtaattttga tatgcgcccc tatttcatta tttttgatga ggtaacagct tttacttcaa
     4801 ctttagataa aaaagaatta caagaaatga atgattattt aattaatatt attatgaaag
     4861 gtcgtcaagc tggtgtattt atgtttttaa cagcacaaag accagatgca gatgtgatta
     4921 aaggtaatgt gcgtgaccaa ttagggttac gtgtgtcttt aggtaatttg tcaaatgatg
     4981 gttatcgtat gacctttggt caaactgata aagaatttca aacgattcat gatagtgata
     5041 tcggccgagg ttatatttct atacttggac aatataatga accaatttta tttgatgcac
     5101 ctttaatgga acaatatgat tttgtggaag atgtgaaaca aatattaaat aaggagtgat
     5161 ttgatgaagc ataaaagtgt gtatctttta tgctttttaa ttttgcctat tctgctttta
     5221 acatctgtga gtgcttatgc tgtttctaat ccagttggag agccctcaca tgcagttaag
     5281 ccaaaaattg aaaagtatga tttatcacat tatcgttcaa tatatagtga aaaaggcgac
     5341 tggaatccgt ttggtcaaga agaaatttca cgtcaaatta ataatgtgtc agattttttc
     5401 ttttctatga ctaagatttt agcaggtgtt acagattatg cgattgaaaa tttatttcaa
     5461 ttagatgtta ttaatgattt tgcggataaa atcggcgatt ttgttggcga tatatatcaa
     5521 aagttattga gtaatttagc attaacatta tttattattg tttgttttaa cgcatttatt
     5581 atttttagtg tacaaggtaa tgcaagagaa gcattaaaac gagcgttttt aattatgtgt
     5641 ttaattggtt ttggtgttgg tatacttgcg aatgcaggta atattatacg tggtacaaac
     5701 aatattggta aggacttaaa taatataatt atgaatagta catcttctat tgatggtaat
     5761 attgattata ttcatgagaa ttcaggtatg aatcatatac gtaatcaatt atttgatatg
     5821 acgatatata gaacatattt agtaatgaat tatggaactg tcgacgaaaa agagataaaa
     5881 gcaaaaggta aagaccgtat tgataacatt ttaaaacttg attatgataa aaaatcagaa
     5941 aaagaattag atgatattgt taaagatgag gttgaaaaga aaaataataa atatatgacg
     6001 caaggttatg tatttcaaaa gttggctatt tcagtgattg gttttattat tacacttttt
     6061 atgtcgatag tatttttatc aataagtttt gcgaagttga ttttttctac atttgcttta
     6121 tttttattct tatttttagt attcagttgg atagtgagtt ttataccagg atttgaatta
     6181 tctgttttct ctgcgtttgc aaaaacgtta ggctatatta ttttaagtgc ttgtatgaca
     6241 ttcttatttg tgatagttgg tttgtgtatc aagcttgcaa atagttttat tgaaccggat
     6301 agtcaaaatg cttatttttt aaattcaata tttataattg taatattgtt tgtaatgtat
     6361 aaaaaacggg ctcaaattat caattttgta agtcgtggta atattagttt ttctcctagt
     6421 gcaattggtg ctggtgtgat gaatcgaaca caagaacgtt ttaataagat tcgtcatgaa
     6481 caagcgcaaa ataagaaagc taaacgagaa aatcaaagag atgaacccgc cccaccactt
     6541 caaaatgata atgatttaag aagaagacaa caggataaac caatgccttt atttataaat
     6601 aaagataatc aaaaaaatgg taataagcgc agagaacaac aagagtcaat gaatggtaac
     6661 gatgtaaaat ctgcttctgt tgaatcaaat gcaaataatt attctaaaca accacaaaaa
     6721 gcatcacagc aagaacatca agtacgagaa acaagacagc gaaaagatat acaaaggtcg
     6781 ccacaagtgg ttaatcagcc attaaataat gagaatcatt caataaatcg taaagaacag
     6841 aagtcagttc aaactgctta tgatactgat gttcaaaaac gacaaataca aaatgcgaca
     6901 cagaatcaac aaagtagaca atctggaaat cgtaatcaac ctattacgcg taattctcag
     6961 tcaaaagacc gtttaaaaga gcaaaaggat atcaataagc atggtaaata aaaaagatat
     7021 tgctaaaaaa gtaataggta aaacgccaat aggcgttaaa ttgaaattag cgaaagtcat
     7081 catcatttta tgtgtggtgg ctttttttat atttccagta attataatgt ttttgttagc
     7141 gcctgattta aataaaggta aagatgatgc tggttgtact gttagtggcg gtaatgtttc
     7201 ggcaaatggt attgataagt ttaacgaaaa tgctaaaggt ggaaaattag agggtaaagg
     7261 aaaagaaata cagaaaattg cagaaaaaaa taaagtacct gtgaatattt ttatggccat
     7321 tattgcgagt gaatcacaat ggggtaaagg agaaaatgcg acaagacaaa ataatccttt
     7381 gtctgttatg ggttcaaaat cgattcatga ttcaacatat ccaactattg aagatggtct
     7441 taatgcggga gctaaaaatt tgtatgatgt ttatatttct aaaggtttag atactcctaa
     7501 aaaaataggt cccaaatatg cgcctgttgg tgcgtcaaat gaccctaata atatgaatgc
     7561 ccgttggata ccaacagttg aaaaaattat gaaagattta ggcggttcag aagctaaaac
     7621 gtcatgtagt aatggtaaag gaaagtctat aaagtttaat ggtaaattgc cacattggtc
     7681 aaatgatgac cctggaaaag gtaatttata cactgctggt caatgtactt ggtatgcata
     7741 cggtatgcgt caaaaaatgg gtaaacctgt atcgacttat tggcatgatg cacataaatg
     7801 gaatgataga gctaaagctg agggttataa agttgataaa aatccggaac ctggtgcatt
     7861 atttattgct gaacaaggtg caggcggtca tgatgggcat tatggtcatg tggctgttgt
     7921 tattggtgta agtgatggtg gtaaaacatt tagaataaca gaaatgaatt gggaaggtgc
     7981 ttttaaagtt aatgaacgta cattgaaaat gactgacgga tatagtttta ttcatgataa
     8041 ggagtgaaaa taaatgaagc aaaaaattat tatgtttact cttgtaacag taatattatt
     8101 ttgtgctgtt ttaattggtt atcagattcc taaacaacaa gttaaaatga aacaaaatca
     8161 aattgaagat ttgcaggaag aacaacgtat attaagagat aaaaatggag agttaaataa
     8221 attagtaaag agacaatcaa aaacagttat ttctgatgaa gaaaaacaaa tacgtgaagt
     8281 ttcttcaaat tttgttaaac agatgtttga aatgaaaaaa gattcttctt ttaaatcaaa
     8341 agcaccacaa ataaagcctt tagttacaaa ggattattac gatacattat ttaaggattc
     8401 taaagataaa tatgatttat atgatgatat aacggttaat gatattcatg tttattttga
     8461 tacttatgac cctaaaaaag atagttataa agtttttgtt cagttcgacg aacgtattga
     8521 aacagatgga gatgataaaa ttgaacatcg tcaaacatct gcacaattgg atttagttcg
     8581 tacagctgaa ggttggcgta ttgataattt aaaacgtttc aatttaaaac ctctaggacg
     8641 ttagtttgtt tcaagacgat agcaatgata taatttacgt aaaaaggagt gaatttatca
     8701 tgaggcgatg gtttgtatta attttaggat tagttatatt attatctgca tgtggtcaga
     8761 aatatgataa agaaattgat gaagttacaa agttagaaaa agaaagtatt caagatgtga
     8821 aaaatacaaa gaagtataaa aatgttgagc ggtcaaaaag ttattataag atttataatg
     8881 atggtgaggt tattataatg acttatatgc cttttaaaga tagtaataca aaagtttcaa
     8941 gagtatataa aataaatcag actagcgata aatatgaaga agattctaat attgatgctg
     9001 aaaagtttga gaaagataat aaaccagttt atgaagaaaa taatatgaaa aaataaagaa
     9061 tcaactccct atacgcttta gcgatggtat cgggagttga tgaacatata tcctcttata
     9121 acttatttta taaataacct ttttaaaagt aaaggaggat atacatgaaa aaaagaaaaa
     9181 aacgtaaaga attagactat aaaacacgtc tagctatcgc aaagtattta aatgatggtt
     9241 taaacatcat tcagatttct aagattttag gtcgtgatga tgcagttctt tatagagaag
     9301 taaataagcg ttctgttgat ggtgtttata atcctttatt agctgaggaa gatgctataa
     9361 aatcgcgtag tatttcagat gaacaagttg ctaaacgtag acgtttgaat gatgaaacaa
     9421 aagcatatat tgaagaaaaa ctttcattaa aatggtctcc tagacagata gcaagtcaaa
     9481 ttgaaaaaga tacagggtat tatataagtt atcctactat atatagatat attcgcaatg
     9541 gattggtaaa agttgatgtt aagcgtgata tgagacaagc aggcaagaaa tataataagt
     9601 cttctgaaaa gcgtggcaaa ttagacgtag ggtctcgtgt tataaagtat cggctcaaaa
     9661 agtattaata atcgtgaaga atttggacat tatgaaattg atactgtttg gagtgttaga
     9721 ccatcaacat attgcttgtt aactattatt gaaagaaaga caagatattt atatgctagt
     9781 agattaaata cacgtaaatc caatgtagtt tgtaatgaga ttataaatat tatgaaacca
     9841 ttattaccca aaagtataac tatggataga ggtaaggagt ttgctctatt tgaagaaata
     9901 gaagatgaat tgaattgtag tatttatttt agtgatttgg gttgtccata tcaacgtggt
     9961 tctattgaaa atgcaaatgg acttttaaga cagtattatc ctaaaggtac ggattttgca
    10021 aatataacac aaaagtcatt agatgaagct gttaaacaaa ttaatgcacg gcctcgaatg
    10081 atatttgact ataaatcgag tgaggaaatg ctaaagtacc atgtaagcac acaaaattgt
    10141 gaacctatat taaatgactg tgtgaggcac gaacccgtca attaatatag gttaatctaa
    10201 gcgaatggtt tacaatgagc gcggaaggac aggacgaata aattttgtat gtaaaatgca
    10261 taatctaaaa agaagcatac tatttaaatc tgtggttttt gacagatatt actagtaaaa
    10321 tgcttctttt tcttcttctg tactctttta gtaatattta ttaaaaaaat ttaagaaatt
    10381 ctcatttttc tattgacaca ctgccattta attaaagcaa ttggagtatt gttactcatt
    10441 gtcggcatcg tcataatgaa tcaatttaac aagaataatt tattactaac tgatcaaaag
    10501 tatttactgt tttggcttct attaggattt atttttggtt tctttccacc tattcaaacg
    10561 acaattaata gtgctttagc tagtcatact cattcaccag cctttgcatc attagtatca
    10621 tttacaattg ggtcaatagc gctattgatt ttaaccgcta tttttaatcg ttctttaaaa
    10681 ctaaaaacaa gtcatttaaa attcggtaaa ttaaagccta tctattttac tggcggtata
    10741 cttggcatgg cttttgtaac agctaacatt atcttaatgc ctcatatggg tgcagcatta
    10801 acaacactta ttgggatgtt tggccagatt ctaatgggca tattgataga tcactttgga
    10861 ttatttggtt cacctaaaat agcaatgaca tccagaaaaa ctattggtct attatgtatt
    10921 ttgacaggca ttatactttt aagattattt taaattaact ttt
//
LOCUS       DALAAR010000078        10891 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000078 DALAAR010000000
VERSION     DALAAR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.080"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(166..435)
                     /locus_tag="O2458_002221"
     CDS             complement(166..435)
                     /locus_tag="O2458_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888070.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            749..1435
                     /gene="mtnN"
                     /locus_tag="O2458_002222"
     CDS             749..1435
                     /gene="mtnN"
                     /locus_tag="O2458_002222"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCZ3888071.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            1455..1982
                     /locus_tag="O2458_002223"
     CDS             1455..1982
                     /locus_tag="O2458_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCZ3888072.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1983..3083
                     /gene="yqeH"
                     /locus_tag="O2458_002224"
     CDS             1983..3083
                     /gene="yqeH"
                     /locus_tag="O2458_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCZ3888073.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3097..3903
                     /gene="aroE"
                     /locus_tag="O2458_002225"
     CDS             3097..3903
                     /gene="aroE"
                     /locus_tag="O2458_002225"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCZ3888074.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3907..4197
                     /gene="yhbY"
                     /locus_tag="O2458_002226"
     CDS             3907..4197
                     /gene="yhbY"
                     /locus_tag="O2458_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCZ3888075.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            4200..4769
                     /gene="nadD"
                     /locus_tag="O2458_002227"
     CDS             4200..4769
                     /gene="nadD"
                     /locus_tag="O2458_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCZ3888076.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4759..5343
                     /gene="yqeK"
                     /locus_tag="O2458_002228"
     CDS             4759..5343
                     /gene="yqeK"
                     /locus_tag="O2458_002228"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCZ3888077.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            5344..5697
                     /gene="rsfS"
                     /locus_tag="O2458_002229"
     CDS             5344..5697
                     /gene="rsfS"
                     /locus_tag="O2458_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCZ3888078.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            5700..6416
                     /locus_tag="O2458_002230"
     CDS             5700..6416
                     /locus_tag="O2458_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3888079.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            6456..7142
                     /locus_tag="O2458_002231"
     CDS             6456..7142
                     /locus_tag="O2458_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCZ3888080.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            7234..7695
                     /locus_tag="O2458_002232"
     CDS             7234..7695
                     /locus_tag="O2458_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCZ3888081.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            7700..9901
                     /locus_tag="O2458_002233"
     CDS             7700..9901
                     /locus_tag="O2458_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCZ3888082.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            9958..>10891
                     /gene="holA"
                     /locus_tag="O2458_002234"
     CDS             9958..>10891
                     /gene="holA"
                     /locus_tag="O2458_002234"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCZ3888083.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYM"
BASE COUNT         4032 a         1350 c         1971 g         3538 t
ORIGIN      
        1 aaatataaag tacctgtcat tatgttgtta caaataaaaa cagcagtaag gatgctaaca
       61 cttagaaaag tcttactgct gttttctatt agaattatct ttaatgcaca aaggaattcg
      121 atacgaaaca aatatgatta actgtgtaat tacaaaataa aactactata gtaaaaagaa
      181 gataaagacg atgataataa atgcatcgaa tataattttc ttgatgtttt tatcaacaac
      241 atcaggtaag gacataagta taaatcctag aattagagtg tagcgacata tgttcataat
      301 tgttgctgta aactcgatat taaatgaaaa ctttaaaaat atacttataa ataaaatgac
      361 aaacgacgca attgcaataa aactgccgta tgtagctatc tttgttacga gactattttg
      421 attattttca gccattgtaa aatcctctta tttcatcatt attattttaa acatatcatt
      481 ttcctgttat ttaataaagt ttcttgacac gattggttga tgtaattgat atgattagtt
      541 tttgaaaaat agacaaatta gaagcatcga attcactgtt atgagttcaa aaataattat
      601 ttatgatacc aagttattga aagagctttt tataaaaata aagcaaaagt tttatacagt
      661 aatgaaaatc atgatacttg tcgttgtagt gattaaaatg tgataaaata acaaatgcta
      721 ggatttttta aaagaggtga atcaaaagat gattggtata attggtgcca tggaagaaga
      781 agtaacaata ttaaaaaata aattaacaca attaagcgaa atttcagttg cacatgttaa
      841 attttatact ggcattttaa aagatagaga agtagtgatt acccaaagtg gcattggaaa
      901 agttaatgct gcaatttcta cgacattatt aattaataag tttaaaccgg acgtcattat
      961 taatacaggt tctgctggag ctttagatga aagtttaaat gtaggtgacg ttcttataag
     1021 tgatgatgta aaatatcatg atgcagacgc aacagcattt ggttatgaat atggacaaat
     1081 accacagatg ccggtagcat ttcaatcaag taaaccttta atagaaaaag tatctcaagt
     1141 tgtacaacaa caacaattaa cagctaaagt aggcttaatt gtaagtggtg atagctttat
     1201 cggtagtgtt gaacaacgcc aaaaaattaa aaaagcattt ccaaatgcga tggcggttga
     1261 aatggaagca actgcaattg cacaaacatg ttatcaattt aatgtaccat ttgttgtagt
     1321 tcgtgcagtt tcagacttag caaatggaga agcggaaatg agcttcgaag catttttaga
     1381 aaaagcagct gtatcatcaa gtcaaactgt tgaagcatta gtgtctcaat tataaaaagg
     1441 taaaggtgag ttaaatgggt ttagttcgca agttttttat gccgaattca tatgttcaat
     1501 caatatttca aattgattta gacaagttag tggacaaagg cgttaaaggt attattacag
     1561 atttagataa tacgctagta ggttgggatg ttaaagaacc tacagaacgt gttaaagcat
     1621 ggtttaagga agctaatgaa aaaggaatca ctattacaat cgtgtctaat aataatgagt
     1681 ctcgtgttgc tagttttagt cagcatttag acatcgattt tatttttaaa gcgagaaagc
     1741 caatggggaa agcgtttgat aaagcaataa ctaagatgaa tatcagacca gatcaaactg
     1801 ttgttatagg tgaccaaatg cttactgatg tatttggtgg taatcgtcga ggtctatata
     1861 caattatggt tgttccagtt aaacgaactg atggctttat tactaagttt aatagattaa
     1921 ttgaaaggcg attattacgt catttcagta aaaaaggtta tatcacatgg gaggaaaatt
     1981 gattgtctga cattttaaaa tgtatcggtt gtggtgcgcc acttcaatct gaagataaaa
     2041 ataaacctgg ttttgtacca gagcataata tgtttcgtga tgacgtgatt tgcagacgtt
     2101 gtttccgctt gaaaaattat aacgaagttc aagatgtagg attagaaagt gaagactttt
     2161 taaaattatt atcaggactt gcggataaaa agggtattgt cgtcaatgtc gtggatgtat
     2221 ttgactttga aggatcattt attaatgcag ttaaacgtat tgtcggaaac aaaaaaatca
     2281 ttttagcagc aaataaattg gatttattgc cgaaacaaat taataaacgt cgagttaaag
     2341 aatggttaaa acgaacagca agaaaatatg gtttggaagc tgacgatgtc gtattaattt
     2401 cagctgaaaa aggctggggc atagacgact tattatcatc aattgcgaat attcgagaaa
     2461 atgaagatgt gtatattgta gggacaacga atgttgggaa atctacattg attaataaac
     2521 tgattgaagc tagtgttggt gaaaaagatg tagtaacaac ttcaagattc cctggaacaa
     2581 ctttagatat gatagatatt cctttagatg aaacatcatt tatgtatgat acaccaggta
     2641 ttattcaaga tcaccaaatg acgcatttag ttagtgaaaa agaattgaaa attattatgc
     2701 ctaagaaaga aataaaacaa cgcgtatatc aattaaatga ggcgcagaca ttattcttcg
     2761 gcggtctagc gcgcatagat tatgtatcag gtggtaaacg tccgttagtt tgtttctttt
     2821 ctaatgactt gaatatacat cgtactaaaa cggagaaggc taatgattta tggcgtaatc
     2881 aacttggcga tttattaacg ccacctggaa atccacaaaa ttttgatctt aatgaggtaa
     2941 aggctgttag acttgaaaca ggcaaagaga aacgcgatgt tatgatctct ggtctaggct
     3001 ttataactat aggaccaggg gctaaagtaa tcgttcgtgt tcctaaaaat gttgatgttg
     3061 tattaagaaa ttctatttta taaggtgatt aaaaaaatga aatttgcagt tataggaaat
     3121 cctatttcac attccttgtc gcccgttatg catagagcaa attttaattc tttaggatta
     3181 gatgatactt atgaagcttt aaatattcca attgaagatt ttcatttaat taaagaaatt
     3241 atttcgaaaa aagaattaga aggctttaat atcacaattc ctcataaaga acgtatcata
     3301 ccgtatttag attatgttga tgaacaagcg attaatgcag gtgcagttaa cactgttttg
     3361 ataaaagatg gcaagtggat agggtataat acagatggta ttggttatgt taaaggattg
     3421 cacagcgttt atccagattt agaaaatgca tacattttaa ttttgggcgc aggtggtgca
     3481 agtaaaggta ttgcttatga attagcaaaa tttgtaaagc ccaaattaac tgttgcgaat
     3541 agaacgatgg ctcgttttga atcttggaat ttaaatataa accaaatttc attagcagat
     3601 gctgaaaagt atttagctga attcgatatc gttattaata caacaccagc gggtatggct
     3661 ggaaataacg aaagtattat taatttaaag catctttctc ccaatacttt aatgagtgat
     3721 attgtttata taccgtataa aacacctatt ttagaggaag cagagcgcaa gggaaaccat
     3781 atttataatg gcttagatat gtttgtttac cagggtgcgg aaagctttaa aatttggact
     3841 aataaagatg ctgatattaa ttctatgaaa acagcagttt tacaacaatt aaaaggagaa
     3901 taacatatgc ttactggcaa acaaaaaaga tacttaagaa gtttagcaca caatattgat
     3961 ccgatttttc aaattggaaa aggcggtatc aacgaaaata tgattaaaca aatagatgat
     4021 acgttagaaa acagagaatt gattaaagta catgtactac aaaataactt tgatgataaa
     4081 aaagaattag ctgaaacatt aagcgaagct actcatagtg aattagtgca agtgattgga
     4141 tctatgatag tgatttatag agaatctaaa gataataaag aaattgaatt gccataataa
     4201 tgaaaaagat agtactttac ggcggtcagt ttaaccctat ccatactgca catatgatag
     4261 tagctagcga agtatttcat gaattacagc cagatgaatt ttatttttta cctagtttta
     4321 tgtctccatt gaaaaagcac catgatttta tagacgttca gcacagatta acaatgatac
     4381 agatgattat cgacgagctt ggttttggag atatttgtga cgatgaaatt aaacgtggtg
     4441 gtcaaagtta tacctatgac acgatcaagg cattcaagga gcaacacaaa gacagtgagt
     4501 tgtactttgt tattgggacg gatcagtata accaactaga gaaatggtat caaattgaat
     4561 acttaaaaga aatggttact tttgtagttg taaatcgaga caaaaatagt caaaatgttg
     4621 aaaatgctat gattgcaatt cagataccta gggtagatat aagttcgaca atgattcgac
     4681 aaagagttag tgaagggaaa tctatccaag ttcttgttcc taaatccgtt gaaaactata
     4741 ttaaggggga aggattatat gaacattgaa aaagcaaaac ggcttgcaaa agagaaactg
     4801 ccagagaaac gttataatca ttctttaaga gttgcagaaa cagcgattaa attggcagaa
     4861 atttatgatg gagacactag taaagtagaa ttagcaggtg tattacatga tttctgtaaa
     4921 tatgatgatt taggtaaaat gtatcaaatt gttcgacaat acgaattagg taatgatcta
     4981 ctgagttatg gtagtgaaat attgcatggc cctgtgtgtg cagcaatcat ggaacatgaa
     5041 tatggtatca atgatgagga agtattaatg gctatcaaat accatactac tggacgtcaa
     5101 caaatgacaa aaactgaaaa actgattttt attgcagatt acatcgaacc tggaagaaca
     5161 atcccaggag ttgatgatat tcgagatatg gcatacaatc aaggtagttt agataagaca
     5221 atttatgaaa tttctaaacg cacagtacta tttttaatac agaaagatat tacggtatat
     5281 aataagacga ttgactgttt aaattattat aactatagtg acgaaagaat aaaggatgat
     5341 taaatgaatt cacaagaatt attagcaatt gctgtggatg caattgacaa taaaaaaggc
     5401 gaagatacga tttctttaga aatgaaaggt atcagcgata tgacagatta ttttgttgta
     5461 acgcacggaa ataatgaacg acaagttcaa gcgattgcta gagcggtgaa agaagtagcc
     5521 aatgaacaaa atatagaagt aaaacgtatg gaaggataca atgaagcgcg ttggatatta
     5581 attgacttag ctgatgttgt ggtacatgtt ttccataaag acgaaagaaa ttattataat
     5641 attgaaaagt tatatcaaga tgcaccatta gaatcatata gtcaggttgc gtattaatta
     5701 tgtcgcaata tgcagaaatg agcctagtgt acgatcaatt gactcaagat caaccatatg
     5761 aaaaatggtt tgaaattgta aaaaatcact gcaaagatga atcaaatatt ttagatattg
     5821 gatgcggtac tggtagttta acagttcaat tagaagcttt aggtaatgtt actggtatgg
     5881 atttaagtgt tgatatgttg actgttgcag ctcaaaaatc agcaaatgta aattggcttg
     5941 aaggagatat gacatctttt gatttgcaac aacaatttga tatcatcact attttttgtg
     6001 actctctaaa ttatttgcaa gatgaaactg cagtgattga aacattcatc aatgtttatc
     6061 atcatctgac tgattcaggt gtatttattt ttgatgtaca tactgtttat aaaatgatga
     6121 ctttgtttaa taatcaaagt tatattgatg ataaagggga cattttttta gcatgggatg
     6181 ctgtgcaagg agatttacct ttaagtgttt atcatgatat gacatttttc attcgacatg
     6241 aagatgaaac atattcaaga tttgatgagt ctcattttca gagaacattt gatgaaaaga
     6301 catatttgtc ttggttagct caagttggtt ttaaacatgt tgaaacattt actgatttta
     6361 atatagatga acataatgaa gatgcagaaa gattgttttt cattgcgaaa aaataaaagt
     6421 aaaaccactc gctcagacgt gtataaagtt gaggagtggt tttattgtat caatttttat
     6481 tacgctataa agatttttta actcaatgga agttatatat tctaagtgct gttgttttaa
     6541 ttatggtatt aattggtttt atattctgga gacaagatga ttatacttca agaaattttg
     6601 aaaataaaga tactgctctg aaacaaagca ctagtgaaaa tagtagtttg tccaaagtag
     6661 aagatgtcca ggtcaaagat ggagataatt ccaaaaataa gggtcctgta tatgtcgatg
     6721 taaaaggtgc tgttaaacat cctaatgttt ataaaatgac atctaaggat agagtagttg
     6781 atttacttga taaagcacaa ttattggagg atgcagatgt aagtcaaatt aatttgtctg
     6841 aaaaattaac agatcaaaaa atgattttca tacctcataa aggacaaaag aatgttgaac
     6901 cacaaattga agtaaacagt gtgcacgaaa aaaatgggaa cacaaataat actaaagtaa
     6961 atttaaatac ggcatctgta tcagaattga tgtctgttcc tggagttggg caagctaaag
     7021 ctaatgcaat tgttgaatat cgcaaccaac aaggtgcatt tcaagaaatt gacgatttga
     7081 aaaaagtaaa aggttttgga agtaaaactt ttgataaact gaaatcttat ttcacgacat
     7141 aatggattat tttaattgca aattttattt tttgtgatta atcttatatt attagaaaat
     7201 gttactaaat tttaagtatg gaaggtgaac atcttggaaa gaatcaaatg ggaagaatat
     7261 tttatggcac aaagtcattt gctagcatta cgttcaactt gtcaaagatt atctgtaggt
     7321 gcaacgattg ttaaggataa tcgtattatt gctggtggtt ataatggctc tgtagctggc
     7381 gaggtgcatt gtatagatga aggatgttta attgaagatg gacattgtat cagaacgata
     7441 catgcagaaa tgaatgcttt attacaatgt gcaaaacaag gtgtatctac tgaaggtgca
     7501 acaatctatg ttactcattt tccatgccta aattgtacaa agtcaattat tcaagcaggt
     7561 ataaagcgta tctactatgc agaagattat cataaccatg aatatgcaac taaattactc
     7621 aaacaatctg gtattgaatt taaaaaaatt ccattttcac cagaatatgt tgctaaatat
     7681 ctgactaaag gttaatgttt tgctgtttgt cgcgttatca atgattgtag gagtgctttg
     7741 gaattctagc aaagtgctct ctacatttct tttcatttta cttttgtata ttacttatcg
     7801 taaaaataaa atcgtttatg cccctatttc tctcttttta atcattttct ccgcatggta
     7861 tttacattat tcacaacaag caatatttaa ttatatcaat tatattgaac gtaattctca
     7921 gtttaatgag cgtgctcaag taatccaaat tcaacgtcaa ggtagtgaca catataaagg
     7981 taggttgagt ttaaaaaatg aaatatatcc tttcttttta acagataaaa agaattttga
     8041 tttaaagaaa attgaaagtc gtaattgtat tgttaaagga caattcaaag ttaatgacaa
     8101 taagtttgta actcttaaat tacaaagtat agttgtacaa agctgcctag aatcgaaccg
     8161 gtctaattta attgagaaac ataaacagtt tataatgaat cgaatttatg attcgggtat
     8221 taagtttccg gatcgtatta tggcattgat tactggtgac gtaaaagaag ttaatgagca
     8281 atttaaggaa cgtgttaaag agatagttat atatcatttg ctggcagtta gtggctcgca
     8341 tatagctgca attgtatttt taatttacca acctttaaaa cgattaaatt tacctttatt
     8401 tgtcattaaa ggaattacaa tcattgtatt agctttattt gctcaataca caaattatgc
     8461 acctagtgct gtaagagcta taataatgac aactcttgta ctggttatta ctaagcaaat
     8521 taaaataaag agtattcagc tattagcatt tgcatttata attatgttta ttttaaatcc
     8581 actagttgtt tatgatattg gatttcaatt ttcattcatc atttcatttt ttattatgct
     8641 actttttcct tttttacagc aattgtcaaa gttacaatca ttattcataa ttacgtttat
     8701 tgcacaatta gcttcattta tcgttgccat tccaagcttt catcaacttc aatgggtggg
     8761 atttttatct aatttgattt ttgtaccgta ctattcgatt atattgtttc cgctatctat
     8821 tttattcttt attacaagtc attttattgt gggattaacg ccgctaaatt acttggttga
     8881 cctaagtttt aattttcatg actggttact agacctattc acaagaatca agcaatcaca
     8941 tttttctgtt cccaagttta atgattggat atttatagta tttataattt ctgtttatta
     9001 catattttgg ttattggcta aacgtaaata tatattggtt acgttttgga ctataattat
     9061 tctgacatta ttaataacgt ttccaacaaa ttcacatcac aaaattacaa tgttaaatgt
     9121 ggggcaggga gacagtattt tatatgaagg cggtaagaac caaaatgtct tgattgatac
     9181 aggtgggaaa gtgattgatg atactaaaca acctagttat tcaatttcta aatatcatat
     9241 tttaccaacg ctaaatgaaa gagggataaa tgaattagag tatctaattt taacacatcc
     9301 acacaatgac catattggtg aagtggaata tattattagt catattaaaa ttaaacatat
     9361 agtgatatac aataagggat atagtagtaa tacattgatg ttattatcga aattaagcca
     9421 taagtacaac attaaactta tggatgtaag acaagttagt agttttaaac ttggagatag
     9481 tagttttcta ttttttgata gttttattcc aaatagccga gataaaaatg agtattcgat
     9541 tattactatg attacatatc aaaataaaaa agttttatta atgggcgatg ctagtaaaaa
     9601 taatgaatct ttactactaa aaaaatataa cttgccggag attgatattt taaaagtagg
     9661 acatcatggg agcaagacaa gtagttctaa agaatttata gagatgatta agcctaaaat
     9721 aagtttgatt tcttctggga agaacaatat gtatcatctt cctaatatag aagttgttaa
     9781 acgattgcaa aggattcgca gtcgcattta caatagtcaa caaaacggtc aagttacaat
     9841 tgacttagat gataatttaa aagttgattc aagctcttat ggaaatgcaa gtggtttata
     9901 gatacagaaa catgatataa tctattgatg tgtcaaacga atgaaagggt gtaatgaatg
     9961 agcgacaata ttgtagctat ttatggagat gtgcctgaat tggttgaaaa acaaagtgca
    10021 gaaatcatat cacaattttt gaaaagtgat agagatgact ttaactttgt gaaatataat
    10081 ttatacgaaa cagagattgc accaattgtt gaagaaacat taacattgcc tttcttttca
    10141 gataaaaaag caattttggt taaaaatgca tatatattta caggtgaaaa agcgccaaaa
    10201 gatatggctc ataatgtaga tcaattaata gaatttattg aaaaatatga tggcgaaaat
    10261 ttgattgtct ttgagatata tcaaaataaa cttgatgaaa gaaaaaagtt aactaaaact
    10321 ctaaaaaagc atgcaaggct taaaaaaata gagcaaatgt ctgaagaaga aataaaaaaa
    10381 tggattcaaa gtaaattaaa tgagaatttc aaagatatca aaagagatgc attagattta
    10441 tttattgagt tgacaggtat taactttaat attgtctcac aagagataga aaagttgatt
    10501 ttatttttag gcgatagacc aacaattaat aagcaggatg ttaaccaaat tattaataga
    10561 agtttagagc aaaatgtatt tttactgact gaatacattc agaaaagaaa gaaagaacaa
    10621 gcaattcatt tagtaaaaga tttaataact atgaaagaag aaccaattaa attacttgca
    10681 ctaattacaa gtaattaccg attattttat caatgtaaga ttctgagtca aaaaggatat
    10741 agtggacagc aaattgctaa aacaatagga gtacatccat acagagtaaa attagcgtta
    10801 ggacaagtaa gacattatca acttgatgaa ttattgaata ttatagatgc ttgtgcggaa
    10861 actgattata aacttaaatc atcatatatg g
//
LOCUS       DALAAR010000079        10365 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000079 DALAAR010000000
VERSION     DALAAR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.081"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            590..1885
                     /gene="purB"
                     /locus_tag="O2458_002235"
     CDS             590..1885
                     /gene="purB"
                     /locus_tag="O2458_002235"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCZ3888084.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1994..2296
                     /locus_tag="O2458_002236"
     CDS             1994..2296
                     /locus_tag="O2458_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888085.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2468..3160
                     /locus_tag="O2458_002237"
     CDS             2468..3160
                     /locus_tag="O2458_002237"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCZ3888086.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3157..5349
                     /gene="pcrA"
                     /locus_tag="O2458_002238"
     CDS             3157..5349
                     /gene="pcrA"
                     /locus_tag="O2458_002238"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCZ3888087.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5353..7356
                     /gene="ligA"
                     /locus_tag="O2458_002239"
     CDS             5353..7356
                     /gene="ligA"
                     /locus_tag="O2458_002239"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCZ3888088.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7369..8568
                     /locus_tag="O2458_002240"
     CDS             7369..8568
                     /locus_tag="O2458_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCZ3888089.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8657..10195)
                     /gene="putP"
                     /locus_tag="O2458_002241"
     CDS             complement(8657..10195)
                     /gene="putP"
                     /locus_tag="O2458_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCZ3888090.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
BASE COUNT         3918 a         1505 c         1909 g         3033 t
ORIGIN      
        1 atttttctat aatcagtttc gtatttatac gattctggag ttcaatatta atgtcttata
       61 tttaaaatgc cacacctatg caatagaata tatattcttt ttgtgagtag aaaatttgtt
      121 atattagaag cgaataattt taaaacatat tatgaaatgt aagaagaaat attatgaaac
      181 taggaaaagt agattgttga aaattccata caataggaga agtaaagtga ataaattctg
      241 gtattactaa agctattgat tgtcctaatt gtaataaaaa gttggatagt gtagttgagg
      301 ggagaaaaac taaaatgcaa aaatatattc tcaatggatc attataaata ccaattagat
      361 taaagtattg cgaacattta caattagaaa tcagtattaa tctgccatag ataattgtat
      421 taatgtaaaa aataaaatag aatgagaata tacggaaatt ttttatacat ttataaccaa
      481 ttctgttaaa ctgtgaagag tagagttaaa aacgaacttt ttgtttattt ttattaaaaa
      541 atgttctgtt tttgaaaatt cgagtataca tacgatagga gttaaaaaaa tgattgaacg
      601 ctattctaga gaagaaatgt ctaatatttg gactgatcaa aatcgctatg aagcatggtt
      661 agaagtggaa attttagcat gtgaagcatg gagtgaatta ggacatatac cgaaagctga
      721 cgtacaaaaa attcgtcaaa acgcaaaggt aaatgtcgaa cgtgcacaag aaattgaaca
      781 agaaacgcgc catgatgttg tagcctttac tagacaagtt tctgaaacgc taggtgaaga
      841 acgtaagtgg gtacattatg gtttaacttc tactgatgtt gtagatacag ctttaagttt
      901 cgttattaaa caagcaaatg acattattga aaaagattta gaaagattta tcgacgtttt
      961 agctgaaaaa gcaaaaaatt ataaatatac attgatgatg ggacgtacac atggagtgca
     1021 tgcagaacca actacatttg gtgtcaaaat ggcattgtgg tacacggaaa tgcaacgtaa
     1081 cttacaacgt ttcaaacaag taagagaaga aattgaagta gggaaaatga gtggtgcagt
     1141 aggtactttt gctaacattc ctcctgaaat agaaagttat gtgtgtaaac atttaggtat
     1201 tggcacagcg cctgtatcaa cgcaaacatt gcaacgcgat cgacatgcat actatattgc
     1261 gacattagca ttaatcgcga catcactaga aaaatttgcc gttgaaatac gtaaccttca
     1321 aaaaaccgaa acgagagaag ttgaagaagc atttgcaaaa ggccaaaaag gttcatctgc
     1381 aatgcctcat aaacgaaatc caattggttc tgaaaatatc acaggtattt caagagtcat
     1441 tcgtggttat attactacag cttatgagaa tgtgccatta tggcatgaaa gagatatttc
     1501 tcattcttct gctgagcgta ttatgttacc agatgttaca atagcactag attatgcatt
     1561 gaatcgtttc acaaatattg tggaccgttt aactgtattt gaagataata tgcgtaataa
     1621 catagataaa acatttggtc tgattttctc acaacgtgtc ttattagcat taattaataa
     1681 aggtatggtt cgtgaagaag catacgacaa agtacaacca aaagcgatga tatcttggga
     1741 aacgaaaaca ccgttccgtg aattaatcga acaagatgag tctattacaa gtgttttaac
     1801 aaaagaagaa ttggatgaat gtttcgatcc taaacatcat ttgaatcaag ttgacactat
     1861 tttcgaacgc gctggattag cataaaatta aataaagtaa cgattcagat ttaataaaat
     1921 ataaatcgct acactttaat agattaatgc gttaaaatga cataaagaat aaaattatag
     1981 gggttgtttc gatatgcaaa ttgaaaaatt acgaggtgca gcgttagatg aattgtttga
     2041 tgcaatacta acgttagaaa atagagaaga atgttaccaa tttttcgatg atttgtgtac
     2101 tgtaaatgaa attcaatcac tgtctcaaag attacaagtt gctaaaatga ttaagcaagg
     2161 ttatacctat gcaacgattg aacaagaatc tggagcatcg actgcaacga tttctagagt
     2221 gaagcgttca ttacaatggg gtaatgatgc ttatacaatg attttagatc gtatgaatat
     2281 tgaaacaaat gaataataaa tgaacgataa acaatggtta tctatctgca ctaataaggt
     2341 agataatcat tgttttttca cgaaaaaatt tacagagtaa aagaacttaa atttcatatt
     2401 aagtctttag aactcgacac ttaaaaatgc tataatcata tgtatgttaa aaaaggagtt
     2461 tcggaaaatg tatgacatta aaaaatggcg ccatattttt aaattagacc cagctaaaca
     2521 tatttcagat gatgatttag atgcgatttg tatgtctcaa acagatgcaa ttatgattgg
     2581 tggaactgat gacgttactg aagataatgt cattcattta atgagcagag taagaagata
     2641 cccattgcct ttagtacttg aaatatcaaa catcgaaagt gtaatgcctg gttttgattt
     2701 ttattttgta cctacagtac tgaacagtac agatgttgta tttcacaatg gtacattatt
     2761 agaagcgctt aaaacatatg gacatagtat agattttgag gaagtaatat ttgaagggta
     2821 tgtcgtgtgc aatgctgata gcaaaatggc aaaacatacc aaagcaaata cagatttaac
     2881 aacagaagat ttagaagcat atgcccaaat ggtcaatcat atgtatcgat taccggttat
     2941 gtatatagag tatagtggca tttatggcga cgtatcaaag gttcaagctg tctcagaaca
     3001 tctaacagaa acgcaacttt tttatggtgg cggtatttcc tcagaacaac aagcgacaga
     3061 gatggcagct attgcagata caattatcgt cggtgatatt atttataaag atattaaaaa
     3121 agctttaaaa acagtaaaaa taaaggagtc tagtaaatga atgcgttatt aaatcatatg
     3181 aatacagagc aaagtgaagc tgtaaagaca acagaaggac cattgttaat tatggcaggt
     3241 gctggttcag ggaagacacg tgttttaaca catagaattg cttatttatt agacgaaaaa
     3301 gatgtctcac catacaatgt tttggctatt acttttacaa ataaagctgc aagagaaatg
     3361 aaagaacgtg ttcaaaaatt agtaggtgat caagcagaag ttatttggat gtcaacattc
     3421 cactcaatgt gtgttcgtat tttacgtcgt gatgcagatc gaattggtat agaacgcaat
     3481 tttacgataa ttgatcctac agaccaaaaa tctgttatta aagacgtctt aaaaaatgaa
     3541 aatattgata gtaaaaagtt tgaacctcgt atgtttatcg gtgcgatcag taatttgaaa
     3601 aatgaactta aaacacctgc agatgctcaa aaagaagcca cagattatca ctcgcaaatg
     3661 gtagcaacgg tttatagtgg atatcaacgc caattgtcac gtaatgaagc gttagatttt
     3721 gatgacctta ttatgacaac gattaactta tttgagcgtg taccagaagt tctagaatat
     3781 tatcagaaca aattccaata tattcatgta gatgagtatc aagatactaa taaagcacaa
     3841 tacacattag ttaaattatt agcaagtaag tttaaaaact tatgtgttgt aggtgactca
     3901 gatcagtcaa tttatggttg gcgtggtgct gatattcaaa atatcttatc atttgaaaaa
     3961 gactatccag aagcgaatac aatcttttta gagcaaaatt atcgttcgac gaaaacgatt
     4021 ttaaatgcgg ctaacgaagt gattaaaaat aattctgaac gtaagccaaa aggactgtgg
     4081 actgcaaata cgaatggtga gaaaattcat tactatgaag caatgacgga acgtgatgaa
     4141 gcggaatttg taatacgaga aattatgaag catcaacgta atggtaagaa atatcaagat
     4201 atggcaattt tatatagaac gaatgcacaa tcacgtgtac ttgaggaaac attcatgaaa
     4261 tctaatatgc catacacaat ggttggtggc caaaagttct atgaccgtaa agaaatcaaa
     4321 gatttattaa gttatttgcg tataattgcc aatagtaatg atgacattag tttgcaacgt
     4381 attattaatg ttccaaaaag aggtgtaggt ccttcatctg ttgaaaaagt tcaaaactat
     4441 gcacttcaaa acaatatcag tatgtttgat gcacttggag aagctgattt tatcggattg
     4501 tcaaaaaagg tgacacaaga gtgtcttaat ttttacgagt taatccaaag cttgataaaa
     4561 gaacaagaat ttttagaaat tcatgaaata gttgatgaag tattacaaaa gtctggctat
     4621 cgagaaatgc ttgaacgtga aaatacatta gaatctcgaa gtagattaga aaacatcgat
     4681 gaatttatgt cagtaccaaa agactatgag gaaaataccc cattagaaga acagtcatta
     4741 attaactttt taacggattt atcgttagta gctgatattg atgaggcaga tactgaaaat
     4801 ggcgtaacac taatgacgat gcactcggct aagggccttg aatttccaat tgtctttata
     4861 atggggatgg aagaatcttt attcccacat attagagcga ttaagagtga agatgatcat
     4921 gaaatgcaag aagaacgtcg tatttgttat gtagcaatta caagggctga agaggtgtta
     4981 tatatcactc atgcgacatc aagaatgtta tttggtcgcc ctcagtcaaa tatgccatcc
     5041 agatttttaa aggaaattcc agaatcacta ttagaaaatc attcaagtgg caaacgacaa
     5101 acgatacaac ctaaggcaaa accttttgct aaacgcggat ttagtcaacg aacaacgtca
     5161 acgaaaaaac aagtattgtc atctgattgg aatgtaggtg acaaagtgat gcataaagcc
     5221 tggggagaag gcatggtgag taatgtaaac gagaaaaatg gctcaatcga actagatatt
     5281 atctttaaat cacaagggcc aaaacgtttg ttagcgcaat ttgcaccaat tgaaaaaaag
     5341 gaggattaag ggatggctga tttatcgtct cgtgtgaacg agttacatga tttattaaat
     5401 caatacagtt atgaatacta tgtagaggat aatccatctg taccagatag tgaatatgac
     5461 aaattacttc atgaactgat taaaatagaa gaggagcatc ctgagtataa gactgtagat
     5521 tctccaacag ttagagttgg cggtgaagcc caagcctctt tcaataaagt caaccatgac
     5581 acgccaatgt taagtttagg gaatgcattt aatgaggatg atttgagaaa attcgaccaa
     5641 cgcatacgtg aacaaattgg caacgttgaa tatatgtgcg aattaaaaat tgatggctta
     5701 gcagtatcat tgaaatatgt tgatggatac ttcgttcaag gtttaacacg tggtgatgga
     5761 acaacaggtg aagatattac cgaaaattta aaaacaattc atgcgatacc tttgaaaatg
     5821 aaagaaccat taaatgtaga agttcgtggt gaagcatata tgccgagacg ttcattttta
     5881 cgattaaatg aagaaaaaga aaaaaatgat gagcagttat ttgcaaatcc aagaaacgct
     5941 gctgcgggat cattaagaca gttagattct aaattaacgg caaaacgaaa gctaagcgta
     6001 tttatatata gtgtcaatga tttcactgat ttcaatgcgc gttcgcaaag tgaagcatta
     6061 gatgagttag ataaattagg ttttacaacg aataaaaata gagcgcgtgt aaataatatc
     6121 gatggtgttt tagagtatat tgaaaaatgg acaagccaaa gagagtcatt accttatgat
     6181 attgatggga ttgttattaa ggttaatgat ttagatcaac aggatgagat gggattcaca
     6241 caaaaatctc ctagatgggc cattgcttat aaatttccag ctgaggaagt agtaactaaa
     6301 ttattagata ttgaattaag tattggacga acaggtgtag tcacacctac tgctatttta
     6361 gaaccagtaa aagtagctgg tacaactgta tcaagagcat ctttgcacaa tgaggattta
     6421 attcatgaca gagatattcg aattggtgat agtgttgtag tgaaaaaagc aggtgacatc
     6481 atacctgaag ttgtacgtag tattccagaa cgtagacctg aggatgctgt cacatatcat
     6541 atgccaaccc attgtccaag ttgtggacat gaattagtac gtattgaagg cgaagtagca
     6601 cttcgttgca ttaatccaaa atgccaagca caacttgttg aaggattgat tcactttgta
     6661 tcaagacaag ccatgaatat tgatggttta ggcactaaaa ttattcaaca gctttatcaa
     6721 agcgaattaa ttaaagatgt tgctgatatt ttctatttaa cagaagaaga tttattacct
     6781 ttagacagaa tggggcagaa aaaagttgat aatttattag ctgccattca acaagctaag
     6841 gacaactctt tagaaaattt attatttggt ctaggtatta ggcatttagg tgttaaagcg
     6901 agccaagtgt tagcagaaaa atatgaaacg atagatcgat tactaacggt aactgaagcg
     6961 gaattagtag aaattcatga tataggtgat aaagtagcac aatctgtagt tacttattta
     7021 gaaaatgaag atattcgtgc tttaattcaa aaattaaaag ataaacatgt taatatgatt
     7081 tataaaggta tcaaaacatc agatattgaa ggacatcctg aatttagtgg taaaacgata
     7141 gtactgactg gtaagctaca tcaaatgaca cgcaatgaag catctaaatg gcttgcatca
     7201 caaggtgcta aagttacaag tagcgttact aaaaatacag atgtcgttat tgctggtgaa
     7261 gatgcaggtt caaaattaac aaaagcacaa agtttaggta ttgaaatttg gacagagcaa
     7321 caatttgtag ataagcaaaa tgaattaaat agttagaggg gtatgtcgat gaagcgtaca
     7381 ttagtattat tgattacagc tatctttata ctcgctgctt gtggtaacca taaggatgac
     7441 caggctggaa aagataatca aaaacataac aatagttcaa atcaagtaaa agaaattgca
     7501 acggataaaa atgtacaagg tgataactat cgtacattgt taccatttaa agaaagccag
     7561 gcaagaggac ttttacaaga taacatggca aatagttata atggcggcga ctttgaagat
     7621 ggtttattga acttaagtaa agaagtattt ccaacagata aatatttgta tcaagatggt
     7681 caatttttgg acaagaaaac aattaatgcc tatttaaatc ctaagtatac aaaacgtgaa
     7741 atcgataaaa tgtctgaaaa agataaaaaa gacaagaaag cgaatgaaaa tttaggactt
     7801 aatccatcac acgaaggtga aacagatcct gaaaagattg cagaaaaatc accagcctat
     7861 ttatctaaca ttttagagca agatttttat ggtggtggag atacaaaagg taagaatatt
     7921 aaaggtatga cgattggttt agctatgaat agtgtttatt actataaaaa agaaaaagat
     7981 ggatcgactt ttagtaaaaa actagatgat agcgaagtta aaaagcaagg taaacaaatg
     8041 gctagtgaga tattatcaag gttacgtgaa aatgatgatt taaaagatat accaattcat
     8101 tttgcaattt ataagcaatc aagtgaagat tcaatcacac caggtgaatt tatcactcaa
     8161 gcgactgcag aaaagagtca aacaaagctt aatgaatggc ataatatcaa tgaaaaatca
     8221 gctttattac cttcttcaac agcagcagat tatgatgaaa atttaaataa taatttcaag
     8281 caatttaatg ataatttgca atcatatttt tctaatttca cacaagcagt agggaaagtt
     8341 aaatttgttg ataaaaagcc acaacgatta gtagtagatt taccaatcga ttactatgga
     8401 caagctgaaa caattggtat tacacagtac gttactgaac aagcgaataa atatttcgat
     8461 aaaatcgata actatgaaat tcgtattaaa gatggtaacc aaccacgtgc tttaattagt
     8521 aagacaaaag atgacaaaga accgcaagtt catatttaca gtaattaatt gtaaattaaa
     8581 gataataaaa aggtaccaat gttagtcata attttgatta acattagtac ctttttaatt
     8641 tgttaagact tatgaattat ttttctctaa cgatgtcacg aactttgttt aagtcagttt
     8701 caacaaatgc accaggtttt ttagtaagtt tacttacaac atatgtaacg attacactta
     8761 caataaatcc aggaataatt tcatataagc cgaatatttc gttgatatgt gccaatggtt
     8821 taatccacgc aatccaaacg ataacgacta aggcacctga aaccattcca cttacagcac
     8881 cggcacgtgt caaacctttc cagtaaagtg caaatagcac aagtggactg aacgatgcac
     8941 caaatccggc ccaagcgtta cctactaagt ttagaattgt gtcgtttgga ttccatgcaa
     9001 tcgcgatggc aacaattgct acaactaata cagataatct tccaatcata acaaattctt
     9061 tttggtgcgt tttagctttt tcttcaccac gaattaattt ataaaagtct tccgttagtg
     9121 aactagatgt tacaagtaat tgtgaagaaa tcgtgctcat aattgcagct agaatcgcag
     9181 caagtaagaa accacctaca agaggatgga agagtacttg actcatcacg atgaataatg
     9241 tctcaggatc ttctagttta atatgataag caggtacgaa tgcaatacct gttaaaccaa
     9301 cagccacagc gcctaataaa ccaacagcca tccagctaat acctaaacgt ctagctttag
     9361 gtagcatctt gtgtgattta atagacataa accttataat gatatgaggt tgaccgaaat
     9421 aacctaatcc ccatgaaaat agagagataa ttcctataaa tgataaccct ttaaataaat
     9481 ttaaatttgt aggtttcata gctgctacat catgaaacgt tccccagccg tttaaattca
     9541 tcatagcaac aatagggacc ataaccatcg caattaacat aatgacacct tggaagaaat
     9601 ctgtaattga tacagctaaa tatccaccaa agaaagtata gaaaatgaca atgaaagcaa
     9661 ctaatattaa accgaaatga taatctaatc caaaagcact ttcaaatagt ttaccaccag
     9721 atacgaaacc agaatgtgta tataatgtaa agaatactac gataatcaat ccagaaataa
     9781 tctttaacac attattttta tcgtttaaac gatttttaaa gaaatctggt aatgtaattg
     9841 catctccagc taattcggta taaacacgaa gtctaggagc aacaacaaag taatttatat
     9901 aagcacctaa tgttaaaccg attgtaatcc acatagctga tagaccagtg ctatagacag
     9961 aaccaggtag ccccataatc atccatccac tcatatctga agctccagct gataatgcag
    10021 taatatacgg tccaatacta cgtccaccta acatgtactc gcttaggtta ccagttgctt
    10081 gcttgtaacc gtaaaagcca ataacgatta gtatcaagaa gtagacggca atcataatat
    10141 aagtttgcca attggcatct acttgttgac ttaatgctgt ccccattgta agcatgataa
    10201 acactccctt tatttaattt ataatttagt tacttgaaaa cattatacat aaagataaat
    10261 gtttagaaaa gtaggaaaat taaatttatt ctttttgaaa tacttgcata ccaatataga
    10321 aagcgctttc gtattttttg gatatgagca aatttttata attaa
//
LOCUS       DALAAR010000080        10276 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000080 DALAAR010000000
VERSION     DALAAR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.082"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(435..1589)
                     /locus_tag="O2458_002242"
     CDS             complement(435..1589)
                     /locus_tag="O2458_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ3888091.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(1785..2795)
                     /locus_tag="O2458_002243"
     CDS             complement(1785..2795)
                     /locus_tag="O2458_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCZ3888092.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            2948..3367
                     /locus_tag="O2458_002244"
     CDS             2948..3367
                     /locus_tag="O2458_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3888093.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            3394..3504
                     /locus_tag="O2458_002245"
     CDS             3394..3504
                     /locus_tag="O2458_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888094.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(3574..7344)
                     /locus_tag="O2458_002246"
     CDS             complement(3574..7344)
                     /locus_tag="O2458_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCZ3888095.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQATETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKTYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(7572..8006)
                     /locus_tag="O2458_002247"
     CDS             complement(7572..8006)
                     /locus_tag="O2458_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888096.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(8161..8631)
                     /locus_tag="O2458_002248"
     CDS             complement(8161..8631)
                     /locus_tag="O2458_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCZ3888097.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(8679..9896)
                     /locus_tag="O2458_002249"
     CDS             complement(8679..9896)
                     /locus_tag="O2458_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCZ3888098.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         3261 a         1559 c         1664 g         3792 t
ORIGIN      
        1 taaagattag agtttacaaa aacattaagg gaattaaaat tgaattgtat tatactttat
       61 tcaattgagt taaattgata agatcgtcaa ttgtttgcat ttatctaaaa gaataaataa
      121 gaaaggaatt gagagcgaat ggaaagtatt tgtttataat aataaatagt aacttttgaa
      181 taataattgt ttggccaaag gtagattaat atgaatggtg atatattatt tgagtgctta
      241 agtttcgaag aacaattata agttagcata ttaaaacctt ttgatagaat cattttgagt
      301 gaggtgttta aaggagagga atataactcg gtagtattta cagtctctat agagtattat
      361 ttaaaactta tagaatataa aaaactgaac gtactatgaa tttagttcac gacgtccagt
      421 tcaacatatc atttttattt agtatttaaa tatttagcaa gtctcttcat accttctttt
      481 aaaacatcca tttcataagc ataggaaatc cttacaaatc ctttaccgaa ttctgtaaag
      541 gatgagcctg gaacgattgc taaatgtgtt gattcaagta aatcgacaca aaattcgaaa
      601 tcgtcatcgg tgatatgttt aatacttggg aaaatataaa acgcgccttc aggttgagcg
      661 gtaatctcaa aacctaattt agttaattca gatactaaat aatttcttcg ttctacataa
      721 gcttcgttca tatatttagg agcttctaat ccttcgttaa gtgctgttat acatgctatt
      781 tgagctggaa cattggcaca aatacaatta taggcgtgca taaatgttaa tttatcaatc
      841 aaatattgag gtcctaatag aaaaccaatt cttattccgg ttgctgagtg tgatttactt
      901 aaaccaccaa ttaatattaa ttgatcacga atgtcttcaa attcagcgaa ggatacatgt
      961 ttaccactaa atgtattttc agcataaatc tcatcgctaa taataaataa cggatatttt
     1021 tttaatacat ttacgatatt taaaacttca tttcttttta aaactacgcc agttggatta
     1081 gtcggataat ttaacaagac agcttttgtc tttggagaaa tatgactttc taatgcatca
     1141 ggtgtaattt taaattgtgt tgctgttgta tcaatataaa ttggtttacc acctagtact
     1201 tcgatgagtg gtatgtagcc tgcataaatt ggtcccggta ttataatttc atctccaggc
     1261 tctatgatag aacgtaacgt tgtgtctatt gcttcacttg ctccatttgt cacaataatt
     1321 tcttcaggat cataggaaaa atgataacga tttttgaagt attgactaat tgcttcgcga
     1381 gtttctaata accctttatt gtgagagtat gatgtcttgt cattgttaat agcatcaata
     1441 tatgcctttt tcacaacatc aggcatcggg aagtctggtt ggccaatagt taaattaaca
     1501 caatcatcca aattattcat acgatttgaa aattggcgaa tacttggtgc tcttaaatat
     1561 ttagaattag aatttaaaga aagtttcatt tgtgacacct caaatataaa tcaaatattg
     1621 tctaaaaatt tagaaattaa taatatcata acatactttt aaagcaaaaa agggtaaatt
     1681 acttaagctt tatttgaaaa acgaaaaatg tctaaataca ctgtagtaac tacttttcaa
     1741 aatgaataaa gtggttacag ttaatgtact tagacagtat aaaattatga atctttaaac
     1801 tgtgatggtc tttgtaaatt aattattgga tttgtccatt tacaaacaaa gtttgtagat
     1861 aatacatata cgatgattac agatatactt attaaataaa gataagtcat taatgatata
     1921 ggattatcga atgggtacca ttcaaatcct ctaacaatgc caataattaa accatgtaat
     1981 aaatatacgt atagcgtacg actaccaata taagtatata attttttctt tgttgacatt
     2041 aaatttagaa acgcagtcat tgcgattaat ataattccat ataatataag tcgtttaaaa
     2101 ggactgaata tactctgtcc ttcattttca agtgaagtat atggtgaact tcccaataac
     2161 caatctgcat tgataggatg aatcacgtaa acgataaaaa acaaaataaa ggtaatgata
     2221 gatactggta ttagtttttt atttttaaaa atagccgtat gttttttggt gaaaatgtaa
     2281 cctagataaa atattgggaa aaatacgatt gtccttgaaa tgcttaagta gctatcgatg
     2341 ttatctgaaa aacctgctcc aatagatata ataattgaaa ctgatagcac tttatatgga
     2401 ttaaatcttc taactattac taaaatgaca tgaaagaaaa atagcgtgat caaaaaccat
     2461 aacgcaaata ctgggttaaa aggatcaagt tgtaattcgt cacttttacc tgttaagaaa
     2521 taataaattg aaaagaatgc aaaaaatatc atataaggta ctatcaaacg ttttgaaatt
     2581 ttttctaaat agtatggttt atcaatattt ttcgcgaaat aaccagatat aaacaaaaat
     2641 gttggcatat ggaaactata aataactagg tataatgctg ataaatattt atcgccacta
     2701 gtgtagggtt gtaacatatg tccaaatacg actaataata ttaaaattgc tcttgcgttg
     2761 tcaaaaaaat aatctctctc ttttaatgaa gtcatatttt atctccttta tgtccataag
     2821 actcttacta atttattata ttttgtatta gttatgcaac aaatttgtct attgtgtcga
     2881 atagtattgt aaaaattata aaaaaagcat ataatattat aaataatatg tagtggagta
     2941 gaacaacatg tataaacaac ttgaaaaact tattacactg actaacaatg acttaaactt
     3001 agtgaataga agatttggac aacgcacgga tatcaaatct gaacagctag aacttctccg
     3061 tattttattt aattacgatc gcttatcaca gtatgatttg acgatgaaga ttagcaggga
     3121 acaatctata gtttcaaggt ggattaagaa attagttttg aaagggtaca tcacaagtca
     3181 acaatctagc gaagatttaa gatgtaaaga attaattttg actgatcaag cacgtacatt
     3241 aatttcacaa ataaataatg cacgttgcga attgattgaa gcaagatgtc aatgtttatc
     3301 ggaagtcgaa ttagacaatt taaatcaatt acttgataag ttaaatcaac gacgcatatc
     3361 gttgtaacac gacaatagca aacacataat ttagtgtcgt tgtgcacaca acaaaaattg
     3421 aatcatgaat tacaagcaaa agtagcggtg attgttaaaa ttgatggtaa acaatcaccg
     3481 ctatttttgc ttgtgtatgt ataaaaaagg gatcaaaggt catcccccat gattgatagt
     3541 gggggatgac ttttgatcct atgttcatgt tgcttattta tattgtggga tgtcgaagta
     3601 tttgccgact tcgccaattt tatcatagta gcctttgatg attttagcat tgatgttagc
     3661 ccaatctaca tctgtagcat attggtgtgt tcctggatgt gcaggattcc atctcatttt
     3721 gtaaagtgta ttttgaccag cttttacata tgagttgccg atgaatttag caccaccaac
     3781 gattgctttt gatactgtgt cccaaccagc ttgtttagca tatttaatac cttcacgtaa
     3841 aggatcgtta tcatatgcag caataccaaa tacgttatgg tatttcgtgt ttgagttagt
     3901 tacaactttg ttgttcacta catctgcacc tttcgctaat tgagaagtac cgttacctgt
     3961 ttctaatagg gcatgtgaga taagataaac ttcattaatg ccatacattt gagcagcttt
     4021 gttaaatgca gcaccttggt tttctaatac acctttacct tttaagaatt gattaatttt
     4081 atcaatagaa atattttgtg gttggtctaa gcgtaagaat tgatatttta atgctggatc
     4141 ttgagctaaa cgcttcgtat ccattgcatg cttaacatca ttaaagttag catctgtcca
     4201 cttacctggt acacgttgta cttgtggttt atattgtaaa ccagcttgta tttgagcaac
     4261 ttggtttaat gtcatacctg tttgattata cttaattaat tctttagcta aatcagttga
     4321 tttaatccat gctaatttac cgttagataa tttaccatag taccaagttt gtccattaat
     4381 gacttgttct ttaacaactg cgaatggttg ttcattaaat gcttttaatg agtatttagc
     4441 tgtatcagaa tttggtgtta catagtaata accattacca tttttaatta cataagtgta
     4501 gttataatct ttggcagctg atgtagttgg tttcacagcg gttggtgcag ttaaatcttt
     4561 tgcatttacc caaccagtgc ggttattaat agtaccgtat aaataaacat ctttgcctac
     4621 agatacttgt ttcgttgcat taaatgtacc ttgagcaatg ttattgcctg ttaaaatgac
     4681 ttggttttta gtaccccaag gaaccattga taagccgtta tttgatttat taacagtata
     4741 tttttgagtc gttttaactt ctttgcctaa gttttgaaca tttaagtctt ttacattgaa
     4801 ccaacctaat gggatgttat ggcttgtatt gtttaataat acatacgttt cattaccatg
     4861 agcacgctct tttgttacat agaacgtacg gtctgcatat ttcgcaccgt ttttcgctgt
     4921 tttttcataa acagaagcac gaataccagt gttgtttggt ttaacttgag caatcttgct
     4981 aactgtttga gtcgtttgtg gtttagtaac agtataagct tttacagctg tttttggttg
     5041 tgctactgct tttttaggtg cagcaggtac agctaaatat gttttactta cccaaccaga
     5101 tttaccattt acagttccaa ataaatagat agatttatca atttgttgtt gcttagtcgc
     5161 tttaaaagtt tggttacctg taccagaaac tgcaccagct tcttgtttat aagtgcccca
     5221 aggtactgaa tataatttag tgcctggttt tactgtatat gtttgcatta catttacagg
     5281 tgattttgca ttgttataaa taacgtcacc ttgtttaacc caaccaatta aagttggact
     5341 attgtaatct ttaactaagt agaatttgtt tccacctaaa cttgcttctt ttgttacagc
     5401 aaatgttttt tgaacttctt tcgttggctt accagttttg tcataaactg tagtgaataa
     5461 gccattgttt ttagcattaa tttgagcaac accgtttaat gatgaaactg ttaatttatt
     5521 atttgttgta ggtgttgatg gcttaggtgt tggtgtaggc gtaggtttag cagtatcaac
     5581 taaatatgct ttacttaccc aaccagattt accattcaca gagccatata aataaattga
     5641 tttatcaatt tgttgttgct ttgaagcctt aaatgtttgg tttccagagc cagacacact
     5701 accagcaact tgtttagatg taccccaagg tactgtataa agtttcgtac caggtttgat
     5761 tgaatatgat tgatttacat ttacaggtga tttagctgtg ttgtaaacca catcgccttc
     5821 tttaacccaa ccaaatttat taccagaatt gtaatcttga acaagataga atttttgatt
     5881 acctaatgta gctgttttag atacagcaaa tgttttttga acttcattag ttgctttacc
     5941 agttttgtcg tatacagtag tatataaacc actatttgtt ggtttgattt gtgcgacacc
     6001 attgtttgca gcaactgtta atttaccagt tgatggtttc gacggtgttg ttggttttga
     6061 tggtgtagta ggggtagttg tagattgcgt accccatggc gccactttac ccatttttat
     6121 taaatatttt tcattaatta agtcatataa ttgatcataa ctataattat gacttcttaa
     6181 atatccatgt ggatcggcat ggtcagtacc acctaaatat ttacttacag cgtagtgagt
     6241 ccatactgta ccatttccat catactcagc actgtctggt tttaaaccat aatattgtaa
     6301 ttgtgtagct gcatagtcag catagttatt cattgaacgt gcaaatgaag catagtcgtg
     6361 tgtgtgtacg atttcaacat tgatgaatct agggttaccg actgcaccga caccccaaga
     6421 taagtaatcc gttggtgctg tttcgattat acgatcccca tcaacaaatg catgtacgaa
     6481 tgcgttttga tagttatttt tcatataact aatttcacca tttatcgtcg aacgatcatt
     6541 agctgtatca tgaactacga taccttcagg acgacctacg ccgttacggt atgcgtattt
     6601 agggaagtaa gatgtataat cttcttcaat tttaggtgct tttaagttat ttttacgaat
     6661 gtaatcgtta attgaagagt ttacttgtgg tttatatttt ggcaaactcg tttttggtgt
     6721 tgcagcaact gatcttggtt gtgctgaagc gctaaaagta gttactttag gtgtcgcttc
     6781 agttttagct ttaggtgctg atgtagttgc agctttaggt gctgcggttt tatattgcgt
     6841 ttcaagagct gcaggtttag cagctgattt aattaattct ggattaattt gattttctga
     6901 attatcatct tcatcatcaa ctaaactata accagcattt gtaacattag tgttagtttt
     6961 aggtgctgta gtgcttgttg actttgcaac aggctgcgta ttatttgtag tcgctgattg
     7021 attagcacga gtgtcaccat ttacttgtgc agtatcaact ttttgactta cttgagcatt
     7081 gcctgttttg ttatttgctg tttttggttg gacaatagca gggtcttgat atacttgagt
     7141 gccagaaatg ttttgcgttg gattttttac ctcagctttt gcttgttcag tagttgcttt
     7201 aactttatta ctatctaaaa cgtttttatt agtagtttga tcttgtgtcg tctcagttgc
     7261 ttgaacttga tgtgcagtga ctgctgaacc tacaagcgtt aatgcaacca ttgatggtag
     7321 tttgtaattg aattttttcg ccattctatt tattactcct aacatttatt aattattact
     7381 aacattatag tacctgtttt atatacctgt gcgtatttaa ccaatttgta atatactctt
     7441 aatttacatt ttcactgtaa aaaaatgaaa aaaattttga atctcaatgg tgacgtgaca
     7501 aatcaaatag aattgatgtc aatttaagaa ttggtttatt acaaatgttt ctaaaataga
     7561 tacaactttt tttaattaag cgaggttaac ttttttgtca tttcaatatg ctcgatgcct
     7621 tcctcaagaa atatattacc tctcatttta aagtttaaac tttcataaaa tgggatagca
     7681 tgacattggg cattcatagt agctacgtaa aaaccttcat ctttagctaa tgattctaca
     7741 gcttgcataa gcattctacc cattccttgt ccacgatgtg atttcatcac agctactcgt
     7801 tctattttga cagttgtttc attaataggg cgtattcgag cagtggcaac tggctgtcca
     7861 ttatcatatc caatgaggtg aatagattca gattcatatt catcaatttc actttcctca
     7921 gggacgcctt gttcttctac aaacactttc tttcttatat agaagcaatc ttctaacatc
     7981 ttttgattgt ttacttttga aaacataaaa ttcctccaca tcctaacaaa atcatttaca
     8041 tttaatttac taataataac atagaataac gattgttatt ttcctcaaaa ataattagac
     8101 ataaaaaagc tataaagatg attgttgtga atcatcttta tagctaatta attattaatg
     8161 ttatagtgct tcttttctaa taattgctgt atattgccaa aagattcgca tttgagcaat
     8221 attccaatta ttcattccca ttgtaaatcc tgaaggctta aatatattca catcagttac
     8281 ttctaaagct gctgcaacta aatattgtaa tggaatgcta agtgatttca ttgtttcggt
     8341 aatgccattt tcatcatata cccatgaaaa tggtaactct gattcgaaaa catcaacact
     8401 attgaaaatt tcaggtaggg ctaaaatata acatgcacca tctacaatag gattgtcgat
     8461 actatttttg taataaatta ggagtgcttc atagttttct cgatgctcat gattgacgta
     8521 aaaaaattca cttctaaaat gagccatatc ttggctatga atgatttgtt gttctaacat
     8581 attaaacatg ccatttatat ttgcaatctt ttcgtatgtt tttctactca tgtatgatgc
     8641 tcctttcata aatagtaaat gttaatcgtc atataatatt aatttacaac accattttgg
     8701 ttatttgaag cttgtggcgc ttgttgtgtg ccaccttgat tttgatttga gttttgatct
     8761 gtagcaggtt gttgttgatt gctggaatca ctgttattag ttgaatcact gttctcgtta
     8821 gatgtcttat ctttatctgt cgtatcattt tgttcttttc tcaataaact attatctaaa
     8881 ggcgttaatg gtattaatga accataatga ttaatgacac gttgatctaa gaaatcattt
     8941 ttatcattaa taggtgataa ttctaagtct ttacgaagta agtttgcata tttttgaatg
     9001 ctttcaacac ttggatgata atagtaaata ccatttaaca tatcatcttt accttttaat
     9061 tgcgcagttt taatttcaac atcatttgtt aagtacattt ttgctaaagc tttaatttca
     9121 gagttagtta aattatgctt tgcattttta cctacaattt gaatcacgtt atcaagttta
     9181 tcaatagaat caacttcctg tgctttttgg aataaaatct taattaattc catttgacgt
     9241 tgtccacgtt ttaagtctga atcatgatgt ctagttctag caactgctaa agcctcatca
     9301 ccatttaatt tttggtaccc ttttttaatt ttaatcttac cagtatcatc tgtgttaggt
     9361 tcatttaagt cgtatggcac atcatagtat atgccaccaa gctcatttac agcctcgaca
     9421 aatgctttca tattgactct cacataataa tcaacaggta cattcatggt agcttctacc
     9481 gaatccattg cggcaattgg accaccatat gcatgtgcat gggtaatctt atcgtaatag
     9541 ccaactttag gaatgtagct gatagtatca cgtggaatac taagcattct aatttgatgt
     9601 tttgattgat taaaagtagt taaaatcata gcgtctgatc tagagtgttc agcatcctgt
     9661 cctttttttc ttcttccatc gttatcatcg atacctaaga aaagaataga gataggttgt
     9721 tcttcgggat tgactttatt atctcttaag ttggattgac gattagcatt tttgctgtct
     9781 tgagaagatt cgaatgcatc ttgggacgtt ttaaaaagta acgtagcgaa gactattgga
     9841 acaacaatga gaaccaatgc tagaaggatc aaaaagtatt ttaaaaattt attcatgatt
     9901 gatgctccta tattaaattt ttgttaaatc ataatcataa ttttaaaata taattagttg
     9961 aacgatattt atacaacttc attactaata cattatagcc ttaaattgta gtactttata
    10021 gtaaaaaata caaatgttat gtcaaagttc cttgtaattt gaatgttttg ttaaaatcaa
    10081 aaagcatttt taaaaatata atgcgacttt agtatgatgt cttaaaaaat ttaaaggcgt
    10141 aatataaatt cgagtgattt atacaaagtg ataaggtata attaattttg ttgtatagat
    10201 aacatattag aaaaacttat tactatgtag cgtaaagcat aaatgaatga taagttaatt
    10261 tttgtaagtt gtatta
//
LOCUS       DALAAR010000081        10200 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000081 DALAAR010000000
VERSION     DALAAR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.083"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(72..899)
                     /locus_tag="O2458_002250"
     CDS             complement(72..899)
                     /locus_tag="O2458_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3888099.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(902..1021)
                     /locus_tag="O2458_002251"
     CDS             complement(902..1021)
                     /locus_tag="O2458_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888100.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1133..2134
                     /locus_tag="O2458_002252"
     CDS             1133..2134
                     /locus_tag="O2458_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCZ3888101.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2256..2720)
                     /locus_tag="O2458_002253"
     CDS             complement(2256..2720)
                     /locus_tag="O2458_002253"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCZ3888102.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2733..3914)
                     /gene="ribB"
                     /locus_tag="O2458_002254"
     CDS             complement(2733..3914)
                     /gene="ribB"
                     /locus_tag="O2458_002254"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCZ3888103.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3925..4557)
                     /locus_tag="O2458_002255"
     CDS             complement(3925..4557)
                     /locus_tag="O2458_002255"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCZ3888104.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4564..5607)
                     /gene="ribD"
                     /locus_tag="O2458_002256"
     CDS             complement(4564..5607)
                     /gene="ribD"
                     /locus_tag="O2458_002256"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCZ3888105.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5734..5869)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6088..7590)
                     /locus_tag="O2458_002257"
     CDS             complement(6088..7590)
                     /locus_tag="O2458_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ3888106.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYITTPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            8113..8427
                     /locus_tag="O2458_002258"
     CDS             8113..8427
                     /locus_tag="O2458_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ3888107.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            8427..9719
                     /gene="arsB"
                     /locus_tag="O2458_002259"
     CDS             8427..9719
                     /gene="arsB"
                     /locus_tag="O2458_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCZ3888108.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            9737..10132
                     /gene="arsC"
                     /locus_tag="O2458_002260"
     CDS             9737..10132
                     /gene="arsC"
                     /locus_tag="O2458_002260"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCZ3888109.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
BASE COUNT         3255 a         1797 c         1479 g         3669 t
ORIGIN      
        1 aagatatttt ctaattgaaa attatcttac tgctgttttt tagggattta tgtcccaacc
       61 tttttagaat attaaatttc tacaatttcg tcatcttcaa caataaagcc cattgtattg
      121 acgctgttat ttaagaaagt cagaatataa cgcattactt catcacgttc tggctcattg
      181 tgaacctcgt ggtaaaaacc ttgccaagct ttaaaatata attcaggtgt ttgatatttt
      241 tctttaaact catcaattgc cctagtatca acaattaaat ccttcgttcc atacattaat
      301 agcgttggca ttggttgaat gtcatgaata tgagccatcg tatctttcat cgtctcatta
      361 attgtattat accaatgata cgttgctttt tttaacatta aaccatcgtt aactgtttct
      421 tcaacaattt ctaaattacg tgttaaatct tttggttcta caccaacatt aaaacgtgtg
      481 tcttttgaaa ttttacctat atttgaaaca agtttatctt tacgattttt tccattcttt
      541 tgaagttcta gcataggaga aattaacatc atcccctcga ttggcaattc tactttttca
      601 agtaaattta ataaaatcaa accgccaagt cctaccccta atacataagt aggaatttta
      661 tattcattag ctatctttaa ccagtctagc aaactttcgt gatacgtttg aaagttttca
      721 atttgtcctt tattagctct tgaagtttga ccttgaccag gcaaatctcc cataatcaca
      781 tgatagccat ttcttcttaa catcgtaata acatatgcat atcttcccgt atgttctaat
      841 atattatgag caataacaac gacgcctttc gcatcatttt cagcttccca cttccacatt
      901 attatactgc ccctttttca ttaatcttca ataacataat tatagcaaat tcactatgta
      961 gatttctatt tatagtatta ttgttgtcca tattattata tataaatgaa atcaacatca
     1021 ataatagtgt aattatacat aattattttt gattgttttt gatgaaaacg ctttctcgaa
     1081 tatttttttc atgctaaact tattgtaaac acaagggttt ggaggagtag caatggcact
     1141 attaaagaat ttttttatcg gattatctaa taatagtttt ttaaacaacg cagcaaaaaa
     1201 agtgggccca cgtttgggcg ccaataaagt cgttgccgga aatacaattc cagagttaat
     1261 taatacaatc gaatacttaa atgacaagaa tatcgctgtt acggtagaca atttagggga
     1321 atttgtcggt acagttgaag aaagtaatca tgctaaagaa caaattttaa caattatgga
     1381 cgcgcttcat caacatggcg taaaggcaca tatgtctgtt aaattgagtc agttaggtgc
     1441 agaattcgac ttagaattag cttaccaaaa tttaagagag attttactta aagcaaatac
     1501 ttacaacaat atgcatataa atattgatac tgaaaaatat gctagcctgc aacaaattgt
     1561 tcaagtttta gatcgcttaa aaggcgaatt tagaaatgtt ggtactgtaa ttcaagcata
     1621 tttatacgat agccacgaat tagttgataa gtaccaagat ttacgattac gtttggttaa
     1681 aggtgcatat aaagaaaacg aatcaattgc atttcaatct aaggaagacg tagatgcaaa
     1741 ttacatcaaa ataattgaac aacgtttgtt aaacgcacgc aatttcactt caattgcaac
     1801 acatgaccat cgcatcatta atcatgtaaa acaatttatg aaagaaaatc acattgaaaa
     1861 agatcgtatg gaattccaaa tgctctatgg ttttagatca gagttagcag aagaaatcgc
     1921 aaatgaaggc tataatttca ctatttatgt accttatggc gatgattggt ttgcgtattt
     1981 tatgagaaga ttagcagaac gcccacaaaa cctatctctt gctgtaaaag aatttgtgaa
     2041 acctgctggc ttaaaacgtg ttggcataat tgcagcttta ggagctacag ttatgttagg
     2101 tttaagtaca attaaaaaat tatgccgtaa atagagcaag acataaacaa taatttagga
     2161 gtctggaaca ataatcaatg ttctaggctc ctaaatgtta tattggcagt tctgaaacat
     2221 ttataaatct ttgattatga actcacaaca gtgctctatg cttttataga ttttaataaa
     2281 ttagccattt caattgcact tactgctgct tcagcacctt tattgccagc tttcgtacct
     2341 gctctttcca cagcttgttc aatactttca gtcgttaaaa taccaaatat gactggtaca
     2401 ttagtttgat cattcacttt agaaacacct ttcgcgactt cattacaaac ataatcataa
     2461 tgagacgtag caccgcgaat tacgcatcct aatgtaatta ctgcatcata atttcctgat
     2521 gaggctaatt ttttagctac taaaggaatt tcaaacgcac ctggcacaaa tgctacatca
     2581 atattgtctt cattaacatc atgtcgaatc aaagtatctt ttgcaccttc aagtaatctt
     2641 ccagtgataa aatcattaaa tcgactaact acgattgcaa ctttcaaatc ttttccaatt
     2701 aatttacctt caaaattcat gttaaaatcc tcctatatta aatgacccat ttttattttt
     2761 ttcgtttcca tataatcatg attatgtacc gtttctggta cgataacttc aattctttct
     2821 gcaatatcaa tgccatattg ttttaatccc tcaaatttac ttggattatt acttaataaa
     2881 ttgatatgtt cgatgttaaa atattttaaa atctgtgcag caatatgata atctcgcaaa
     2941 tcttcatcaa aacctaatgc taaatttgca gttactgtat catatccttg ctcaattaat
     3001 tcatatgcgc gtaatttgtt taacaatcct atgccacgac cttcttgagg tagataaata
     3061 atcatgccac catgttcatt gatatacttc atagacgatt caagttgagc accacaatca
     3121 caacgttgac tatggaaaat atcgcctgta aggcacgcag aatgtaagcg tacattttca
     3181 tgttgtcgaa ttgcaccttt tgtcagtaca actatctctt catctgtgta tgtcgcttta
     3241 aaaccataca tatcaaatgt tccgaaatct gtaggcattt tcacttttgc cttaaattca
     3301 atttctggtt ctaatttttt acgatattca attaaatcat caatcgtaat catctttaat
     3361 tgatgttttt ctttaaactt ttgtaaatct tgtcctttcg ccatcgtgcc gtcatcattc
     3421 ataatctcac aaatgacacc agcgggcttg gcaccagtaa gtttagctaa atcaacagcc
     3481 gcttctgtgt gtccatttct agctaatacg cctttatctt gtgctactaa tggaaataaa
     3541 tgaccaggac gattaaaatc tttagcttca ctactaggat caatgagctt tttggcagtc
     3601 aatgtacgtt cataagcact aattcctgtt gttgtatcta catgatcaat actcactgta
     3661 aattgcgtac caaagatgtc ggagttatca tcaaccattt gtaccaaatc caaacgttgt
     3721 gcaatatctt tagacactgg tgcgcatatt aatccccttg cttctttcgc cataaaatta
     3781 atggtattat cgttcatcca ttcagtaacc gctactaaat caccttcatt ttcacgattc
     3841 tcatcatcta ctacaataat tggttctcca ttttttaaag ccattaaagc actgtcaata
     3901 ttatcgaatt gcatgctacc cctcctaaaa accaaatgct cttaatttat ctacagataa
     3961 ttggtcttta tctttattta aaatattttc aacatattta aacaaaacgt ctgtttctaa
     4021 atgtacttta tctcctaatt ttttggatga taaaatcgtt gaacgcctcg tttctggaat
     4081 aagatgaatg tcaaaactgt tatcatgctt atcaaatacc gttagactaa caccatccac
     4141 agtaatagac ccttgcttaa ctaactgatt attaatatgt tggctacatt gaatcgtaat
     4201 aatttttgca ttggctgttt catttatttt tgaaactgtt cctagttcat ctacatgacc
     4261 gaggacaaaa tgtccaccaa acctaccgtt accactcatg gcacgctcta aatttacttc
     4321 tgattgtcgc ttaacatctg ctaaataggt tttattttca gtgcctttaa ttacttgaac
     4381 agtaaaagat gtctgattaa aatcaatcac tgttaaacat gcaccattaa cactgatgga
     4441 atcaccaata tgcatatctg ccgtaatctt atgtgcttca atttcaatcg tcctgactga
     4501 ttgacgaatt tgaacacttt taacgacacc tatttcttca acgatgccag taaacatgca
     4561 tcatcacttc tttcgtaaag ttaatttaac attttgattt aataactcgg aatgaacaat
     4621 ttcaaattgg ttcgcatctg gtatctcaat cacatcattt gtttgataaa attgataatt
     4681 tccagatccg ccaattaatt tcggggcata atagagaata aattcatcta tataattaga
     4741 ttggagaaat tctgaagtag tggttggacc tgcctcaact agcaaagttc caactcctct
     4801 tttatataaa ttgtgaagaa ttgttgttaa atcgcaagac ttcaagtaaa taatttcaat
     4861 atgtgtttga ttggttgtta aatttggatt ttcagtatat atccaaattg gtgttgattc
     4921 atcttgataa atttgctgat taaaatgaat attcccagac ttagacaata ttacttttat
     4981 agggtttttt ccatcttgaa tacgtgtagt atattgtgga tcatctaatt caactgtacg
     5041 tcttccagtt aacactgcgt cgtgtcgatg tcttaactta tagacatctt gtttaacctc
     5101 tttgttagta atccattgac tttgtccatt atcattcgct tgtttaccat ctaaacttgc
     5161 agatactttc actgtaattt gtggcagttg ctttgctttt gctttaaaaa agtcttggta
     5221 taattgtgat gcccgttcat catcaacgca ttcaacctca ataccgtgag cccgtaacgt
     5281 ctcatcacca tgtgtgtcta acgaattgtc ttttgttgcg tatactactt ttgctatctt
     5341 acaatcaatt attttgttaa cacagggtgg tgttgaacca aaatgactac atggctctaa
     5401 cgtaatataa atcgtcgcac cttcagcatt ttgttgtgcc atatcaagtg cttgaacctc
     5461 cgcatgcttg tcaccttttc tcaagtgtgc accaatacca acaatcctac cttctttaac
     5521 tacaacagcg ccaacgggtg gattaacacc tgtttgacct tgtaccatat ttgcaagttg
     5581 aatcgcataa tccataaatt gactcaaatg atcacctcta taaacaaaaa tcctcacatc
     5641 atgaattaag atgcaaggag aaaaatttat cgttaaataa gcctatttgt acacattttt
     5701 acaaatacgc tacattatct ttgtcgataa ttaacattct ttctcccatc cagactttaa
     5761 ctgtcggctc tagaatctca ctagatcagc cactaatatg aaacatatta gcaggtcgca
     5821 ggctttattt actgccggtt gggaatttca ccctgccccg aaagaattat atatgaaatt
     5881 gttatagatt atttgagtac gtagtatgtc aactacattt aaaatgatac tatatgtttt
     5941 ctgaaaaaac aattaatgac ggttttaatt taatataatc tgagtactat aggcatctca
     6001 ttgatatgat tcttactaac agacattaaa atcaaacctt caattcgtct ctatagagcg
     6061 ttctctttat tatcttctag ttacaaatta ttgattgtca ctgcgctgtt gttgctcatt
     6121 cgattctaaa gcatcatata attgagatac tgtatgcgca acttgttcta caatcatttt
     6181 cacaccgttt cgtagtttat taacaccgtt tgtcatttga cctatcgcaa tcatatttgt
     6241 taatgttcca aaccttggac taataacttg attggtttcc ggaatgattt gtatgcctcc
     6301 cattgggtgt gcttgtacaa tttgtctatt ttcaagattt ctaattaatt gatcatcttg
     6361 atccaattca tttaaatgac tttttgcacc tgtcgcgtta atgacaacat tatatatgtc
     6421 tactgattct tggtttttgt atgaaaaata atacaacttg ccatcatgtt tcacatcttc
     6481 taaatctttt ttcaaaatta aagacttatt ttctattaat tcaataatta gttcagcagt
     6541 tcttggaggc attggatttg aatttaattg aatcatcttt gagtattttt gattaaattg
     6601 atgttggtct tcaatactta agctattcca tatccaattt aaattctctt tcaaatgttc
     6661 aatcatactt tggaaaatgc ccatttctgt tggacgcgct aaatcatact tcaaatctgc
     6721 aatatgattt cctgtacgtc tatgtactaa ttttttaaaa tcaatgtcat aatcagcaca
     6781 ttcttttaaa aataaagaaa ctaaagtatc aagcggtgca ttgccgaaat gatgtttttt
     6841 aatgtcattt aatttgtctt tagttaagta cttgaatgtc acgtctatca ttgtacctct
     6901 tacacttggt aaatgagcag aacgactcgt catagtaatt ggtaattttg gatgatgagc
     6961 agcaacataa cggacaacat ctaaactggc aaggcctgta ccaataatcg caatatcgtc
     7021 cagttcattt acttcgtcta acgtattata tgttggataa ggcgtagtga tatatccttt
     7081 tttacccttt aagttatatg gatcatggta ggcaaatgta ccacatgtta aaaatacata
     7141 atcgtacgct tgccatgatt gtcctgaatt tgtagtacat atgtaataag ttaaattcgt
     7201 ttcatcgata ttagaatttg tataaatctc ttgaacttta ttataattag ttgatatatt
     7261 tggatatttt ttcgtgaaca tagataaata agatttcata taatgtccga atacaaatct
     7321 cggtaaatat gcaggttcat caaaattaaa atcagtttgt tgtttatacc acttccaaaa
     7381 ttcagtctca tcatctaaat ttaaactcat ctttttcgaa ggcatattaa ttagcagctc
     7441 agaactatca ttttgaaatg gtacgccctg tcccatattt actttatcat cgtataaatc
     7501 tatatctaat tgattaaact tcgggtgctt aactaactct ctcaatacac ttacaccagc
     7561 agttcccatg cctattattg ctacacgcat atatcatcca tccttttgta attcaaaata
     7621 ttatatttaa actattacct ataattatag caatttggta tttccaataa aatttattaa
     7681 ggtaagctat tatttcactt tcacaaccat taaaccattt aaaacttttc atttaatatc
     7741 aaccttagtg tttgtgttaa aatattttta acgaggtgaa ttaaattgaa atttatagca
     7801 aagatattaa tctcattagt aattctattt atgattttta gtcgtaaaaa caaagcttct
     7861 taatcaatac ccttgattac aaaataaatt caactaaggt gattggccat acactcaaga
     7921 tatggctaat cacctttttc aatgcagaac aaagagtata ttttgcaaaa tcaatatcta
     7981 taatttatct ttaataccct tttaatacta aaaccaaatt taaattacat agtcatatcg
     8041 tgtaatatac caaaggaaca tagacattca tctatataga tgataatcta tttaacgtct
     8101 aggaggtatg ttatgacgta taaagaacta gcaacatttt taaaagtttt atcagattca
     8161 agcagattag aaatactaga tttactttct tgtggagagt tatgcgcttg tgatttgtta
     8221 gcacattttc aattctctca acctacactt agctatcata tgaaagcatt agtaaaaacc
     8281 aacttagtta cgacacgaaa aatcggaaat aaacatttat accagctcaa tcataatatt
     8341 tttgagtccg taattaataa cttgtctaag gttcatacct ctaaccaacg atgtatttgt
     8401 cataacctta agactggtga atgctaatga tgacaacttt agcgacactc atttttctag
     8461 taactttatt atttgtatta tggcaaccta aaggcctaga tattggcatt accgcactaa
     8521 ctggtgcctt tattgctgtt attactggtg ttgtaagttt ttccgatgtt ttcgaagtaa
     8581 caggtattgt ttggaatgct actttgactt ttgtctcagt cattcttatt tcattaatat
     8641 tagataaagt tggattattc gaatggtcag ctattcacat gcttcatgct tcaaaaggca
     8701 atggtttaaa aatgttcgtt tatatcatat tattgggtgc cattgttgct gcatttttcg
     8761 caaatgatgg cgcagcgtta atcttaacgc ctattgtatt agcgatggtt aaaaatatag
     8821 gttttagtaa gcgggccata ttccctttta ttattgcgag tggttttata gctgacacaa
     8881 cttctttacc tttaatcgtg agcaatctag tgaatattat atctgctgat tattttcatg
     8941 taggattcgt tcgatatttt agtagaatga ttatacctaa tttattctcg cttttagcaa
     9001 gtattatagt attgtggtta tattttagaa aagcgatacc taaaacgttt gatgataata
     9061 atataaagca tcctaaagat gccattaatg atttaaagct atttaaaatt tcatggattg
     9121 ttctagttat attacttttc ggctatctaa tcagtgaatt tactaaaatt ccggtatcaa
     9181 ttttcactgg aatcattgct tttatttttc taatgttggc tcgtaaatca aatgctgtaa
     9241 atattaagca agtcattaag ggcgcacctt ggaatatagt attattttca attggtatgt
     9301 atatcgtcgt attcggctta agaaatgctg gcattacttt aatattggct aaaatattag
     9361 aatatatttc caattacggt ctatttagca ctattttggg aatgggcttc atttcagcgt
     9421 ttttatcatc aataatgaat aatatgccta cagttttaat agatgcgatt gctattggtc
     9481 aatcaaatgt ccatggcatg ttaaaagaag gcctaattta tgcgaatgtt atcggttctg
     9541 atttaggtcc aaaaattaca ccgataggct ctttagctac attactgtgg ttacacgtct
     9601 taacacaaaa agatgttaag atttcttggg gcacatactt taaaactggt atcatcatta
     9661 caattccagt actatttata accctcatag ggttgtatct aacacttatc atattttaat
     9721 taattaggag attgttatga ctaaaaaaac aatttatttt atatgtacag gcaactcatg
     9781 tcgaagtcaa atggctgaag gttgggctaa acaaatctta gcggatgatt ggaatgtata
     9841 ttctgctggt atcgaaacac acggtgttaa tcccaaagcg atagaagcta tgaaagaagt
     9901 aggcattgat atatcaaatc atacatcaga tttaatcgat aataatatta ttaaaaattc
     9961 aaatttagtt gttacattat gtagtgatgc agacgtaaat tgcccttctt taccaacaaa
    10021 tgttaagaaa gaacattggg gatttgatga tcctgcaggc aagccttggt cagagttcca
    10081 acgtgtaaga gatgaaatta aaatcgcaat tgaaaatttc aaatcacgat gagagtggga
    10141 cagaaatgat aaaaaaacac taatgattta ttatgtagtg gttctttatc attagctata
//
LOCUS       DALAAR010000082        10179 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000082 DALAAR010000000
VERSION     DALAAR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.084"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(71..1129)
                     /gene="hisC"
                     /locus_tag="O2458_002261"
     CDS             complement(71..1129)
                     /gene="hisC"
                     /locus_tag="O2458_002261"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCZ3888110.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1369..2883)
                     /locus_tag="O2458_002262"
     CDS             complement(1369..2883)
                     /locus_tag="O2458_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCZ3888111.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2876..3853)
                     /locus_tag="O2458_002263"
     CDS             complement(2876..3853)
                     /locus_tag="O2458_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888112.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4075..5856)
                     /gene="recQ"
                     /locus_tag="O2458_002264"
     CDS             complement(4075..5856)
                     /gene="recQ"
                     /locus_tag="O2458_002264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCZ3888113.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5868..7751)
                     /locus_tag="O2458_002265"
     CDS             complement(5868..7751)
                     /locus_tag="O2458_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCZ3888114.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(8022..9962)
                     /gene="ltaS"
                     /locus_tag="O2458_002266"
     CDS             complement(8022..9962)
                     /gene="ltaS"
                     /locus_tag="O2458_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCZ3888115.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         3121 a         1848 c         1398 g         3812 t
ORIGIN      
        1 caatgcaagt tggggtggga cgacgaaata aattttgcga aagtatcatt tctgtcccac
       61 tccccactta ctattcgtat ttaaagtttg ataaaacttc taacatttta tcattttgtt
      121 ctttaaaacc aattgtaatt ctaacaccag ttggaaatgg tcgcgtaata caccctacat
      181 ttaaaagtgc ttcataaagt tcatttaccc gctttgtttt tacaaatata aaattcgttt
      241 gacttggcaa gaaatactca ctttgaggta attgataaaa tctttcgcgt tcaacactat
      301 ttttatgtgt tacctctttt aaatattgtt gatcttcaag tgctgcaact gctgcgtatt
      361 cagatatacg tgtcacatta aatggtggtc taatgatgtt ccatttttca atcgcatgtt
      421 cacttgctac cacatatcct acacgtaaac ccgctaatcc atacgccttt gaaaatgtac
      481 gtaataagaa agcattgtca tatttttgtt gcaaagcaag tgtatccggg tagtcctctg
      541 ctgtcacaaa ttcaaaataa gcttcatcta taattactgg tacatgtgga ggtacttgag
      601 ataaaaacga atctaagctc tcatgattaa aatatgtacc tgtaggatta tttggattac
      661 ataaccatac caatgacgta tcttcattaa cttcttttaa aataccttct aaatcgaagc
      721 caccatcttt taaaggtact tgtatcacat tagctgattc aacaatcgca ttgtgataat
      781 attgaccgaa tgtcgcttca cttgtaacaa tagtatcccc tggcgttaat acagctctag
      841 aaatcattaa tataacttca tctaatcccg caccaaataa aatgcgtgat tgatctacat
      901 ttaaatgttt actaatcgcc gcttttaatg tcggtgatcc tgtttcagga taataatata
      961 actcatctaa gtgtgctgat atcgcttctt taactttagg cgatggtcca tacaaatttt
     1021 catttgatgc gagtttatat aaatctcctt caatgccata cttttctttc aatgcccttg
     1081 gagataaacc aggctgatat gctgatagtt gattaagttg ttccttcata ataaaaacaa
     1141 cctccttaga attaactgaa atttcaaaaa acttatattc attatatcgc tctatgtttc
     1201 taaaatgcaa tgctactatt ttcacatcat aatagcttac attgtgttta acatgtcata
     1261 ttttttaatc attattgcat gtttaaattg aacataatta tagatttaaa tataacaacg
     1321 ccacataaga tataatttgg tgttacgcat cttatgtggc gttggtcgtt atttgattaa
     1381 cccttttgct tttaaataat ctttcgcaac tgtatatggg tcttcatttt taactgttac
     1441 tttatagttc atcatttgca tatcttcatc agatattttg ttttctagtt tgtttaacgg
     1501 tttcttaatt tctggatgtt tctttaaaaa gctttcttta aataatggtg ctccttgata
     1561 tggtggaaat acgtgcttat catcttttaa cacaaccata tcatattgtt ttaattcagc
     1621 gtcagttgaa tatgcatcta ttaaattaat attaccttta ttgatcgctt gatatctcaa
     1681 cttaggttcc attgtacgta tgttatctaa atttaaatta taagcctttt gaacagcttt
     1741 gtaaccatct ggacgatcat taaattccaa tgtaaatcct ggtttaagtt gatctttaac
     1801 cttatttaaa tcaccaattg tacgtatatt atgttgttta gcaaaatcac gttttacagc
     1861 taaagcatac gtattgttat acttcattgg ttttaacata gtcatttgat atttcttttc
     1921 aagactttgc ttagcttgtt cataaacttt tttctcttct tttgacttca atggttcttt
     1981 tgttaattca cctaaaactg ttccagtaaa ttctaaatac ccatctatat cgtcagattt
     2041 taaagcatta aataaaaatg ctgttttgcc cataccatct ttcacttcta cagtattttt
     2101 ggtctcttct tctattaaaa ttttatacat atttgtaata atcgatggct cggagccaag
     2161 ctttccagct aacgtaattt tatcaccttt ttgtgcaaac ataggaatag cgatagccag
     2221 tataataatc atcactataa cacctaacgt catcaataac tttttataag ataatttagc
     2281 cataaatctt aaaattaaat caaatataat tgccaataag gctgctggaa ttgcacctaa
     2341 taatatcaac gatgcattgt tacggtctat acctaataaa attaaatctc ctagtccgcc
     2401 tgcaccaatt aatgctgcta gtgttgctgt acctataatt aataccatag ccgttcttac
     2461 accagccatt ataacaggca ttgctatcgg aagttcgact ttagttaaac gtctaaatgg
     2521 tttcatacct atacctttag ccgcttcaat gagtgatgga tcaacttctt taattccagt
     2581 atacgtattc cttaaaattg gtaacaacgc atacactaca agtgcaataa ttgctggcac
     2641 acgaccgata ccaaataaag gaatcattaa acctaataat gccaacgatg gtatggtttg
     2701 aagaattgcc gcaatattca ttacgatttc agatatcgtt ttagtcttcg ttaataaaat
     2761 acctaatggt accgcaatag cagttgcaat caataatgcg ataaatgata tttgaatatg
     2821 ttctatcatt gtcgaaaaga gttgcccctt acgttcactc aatatgtcga aaaagttagt
     2881 catgttgagc tacctccttt ttctgggaca aatatttgaa gatatctttc ctatcaataa
     2941 catattgacc tacgctatct tcttgcatga caatgacacg ctcgctctct gataaaagtt
     3001 gatacaatac ttcaattggt tgattgtcat aaacaattgg ataagcgctc atagatgtaa
     3061 cctcatcgat tggtttcata atatccaagt cacggataat tgcgttctct tcaacacatg
     3121 gcgcatcatc ttctaaatga ctacccataa attgtttaac aaattcactt tgaggattat
     3181 ttttaaatcc ttctggtgtg tcaatttgtt caatatgccc ttcattcaaa agacaaatct
     3241 tatcaccaag tttcatcgcc tcttgaatat catgtgtaac aaatatgatt gtcttcttaa
     3301 ttttagtttg taattcaatt aaatcatctt gaagtttttc tcggctgatt gggtctaatg
     3361 cactaaacgg ttcatccatt aaaataactg gtggatcagc tgctaacgca cgtataactc
     3421 ctacacgttg tcgttgcccc cctgacaatt catcaggttt tctgttttta tatttttcag
     3481 gttctaatcc aaccatttca agtaattcat ctactctttt atctatatct ttttctttcc
     3541 actttttcat ttgtggcact tgtgcaatat tttctttgat tgtcatatgt gggaataatg
     3601 caatctgctg caatacgtat ccaatatccc aacgcatttc gtatactgga taatcactta
     3661 ttggtttatc tttaaaataa atataacctt cacttaagtg aatgagtcga ttaatcattt
     3721 ttaatgtcgt agtttttcca caacctgaag gtccaattag cacaaaaaat tcaccctcat
     3781 taatattgaa actaatgtta tcgacagcaa catgtttgcc ataacgctta gttacatttt
     3841 taaacttaat cactttgcca cctctttttt tctcatagca taaaaccgag attatatgta
     3901 tgtattccct atttaaccac gtttattaca attttcaaat ttaaatgatt tatccttgaa
     3961 cttttttaat aaaataatga ataataggta atctccagtt aagaaatagt gttattttac
     4021 cttgaattca aaaaaacacc cagtaaaaca aggaatgctt actaggtgtc ttcactatac
     4081 tttggcttta taattttgaa tcgtttctaa aaatgctgga caataatgtt ttaatttgta
     4141 actacctacg ccatcaatat taatcatatc ttgtttcgaa gcaggcttac gtttagcaaa
     4201 ttcctccaac gtgtaatcag aaaatatact tacaggtgct atcgttaatt tgtcacttaa
     4261 ctttttacga acttctacca actgactgaa taatactcgg tcaacccctt caaccgtatt
     4321 tataaatact ttttcagtcg ctttttgctt aaatggtgtt gtgaatactt ctacttcatt
     4381 actgagtaat tttttaattg aagtatcaca cattaatatt tcgtcatttt catttaagaa
     4441 ccctttgaat cttaattcat ctattaagtg acttaattct gatgttgtgt aacctttcat
     4501 taaaccatgg gttgaaattt ggtcataacc tttatactta atataatctg ttgactctcc
     4561 tcttaacact tgaatgataa cactataact ctcttgttgt ttcatacgag cgatgcaact
     4621 aataatcatc ttagcttctt gtgtcatatt atatgattta tcttgttgaa cacaattact
     4681 acattgttca cattcttcta atttttcatt cggttcaaaa taatggacaa ttgttgcttc
     4741 tagacatttt tttgtttttg tatattgaat cattttagtt aacttttcgc ccattttatc
     4801 tttatagtca tcatcagctt gagagactgt tataaaatac tcgtgtaaat tgatatcgcg
     4861 ttcgctaaat aacaaaatac attcactttt caacccgtca cgacctgcac gacccgcttc
     4921 ttgataataa gattctaaat ctccaggcat attataatga ataacaaagc gtacattgga
     4981 tttatcaata cccataccaa aagcatttgt agcaacgact actttaacac gatcaaataa
     5041 gaaatcattc tgcgcttctt ctctttcttt attgctcaaa cctgcatgat atataacact
     5101 ttcaattttc tgactttcta aggcttcttg aagctcttca acttgcttac gtgtagaaca
     5161 ataaataata cctgcatctt catcgtgtgt tttaatataa tccaatataa atttttgacg
     5221 ttgataagta ggatttactt taaaaattaa gtttctacgc ttagtactcg ttttaatttg
     5281 atcagtttga gcgatattta acttttctct aatatcttgc tgtacttcaa ccgtggcagt
     5341 tgctgtcaac gctattattg taaaatcttg aggtaacgta aatacttttg aaataacatt
     5401 ttggtaactc ggcctgaaat catgacccca tttagaaata caatgcgctt catcaaacgc
     5461 gactaagtga atctttatac gctgaagcat atttaaaaaa tatcggtttt caaatcgttc
     5521 tggtgcaaca tacaaaaatt gaatttctcc atttgataat gctttttcaa tacgttgttg
     5581 ctctttttga gtcaaactac tatttaaaaa agcagcttga attcccatcg cttttaattg
     5641 atccacttga tctttcatta atgatattag tggacttatt acaattgttg taccacctaa
     5701 caataaacct ggtacttgat agcatataga cttacctcca ccagttggta agacaccaag
     5761 cacattacga tggtctaata ctttgctaat aatttcttct tgtcctggtc gaaacgtttc
     5821 atacccaaag taatgcgata atgtttgttg catcataaat tgacccctta ttgttgttct
     5881 ttaatttctt ctaactcact ccatcttgtg atgtctaaat catattgaat ttcaagttgt
     5941 tctttttctt cgtttaattc tttaattttc ccataatctg cacttgcctc aatcatgagc
     6001 acatcaattt cttccattct tacttccgct tgttctatgc gtttcatcaa ttgttcatat
     6061 tctaattttt ctttatatga taaaccattt ttcttacgta cagttgtaga agatttagat
     6121 tgttgcttca atgtggattt atttttatct aatgattttt tataactttc ataatcttca
     6181 aaagttccga taatcttttc catctgacca tcatgaataa accaatatga ctgtgcaact
     6241 ttatttaaga agtagcgatc atggcttacg gtaatcactg taccaccgaa agtatgaata
     6301 taatcttcaa gtattgttaa agtctcagta tctaaatcat ttgtcggttc atctaacaac
     6361 agaacatttg gctggtgtac gagtagacgt aataaataca aacgcttttg ctctccacca
     6421 gataatttat aaactttttt accatgagtt gcacttggaa ataaaaatcg ttcaagaagt
     6481 tgtgtaatag ataccgaggt tccatctttt tcttttgcga tctcattttc ttctcttaaa
     6541 taatcaatca tacgaatatc tctatccagg gtctcatctg tttgcttaaa ataagctact
     6601 ttaaccgtct gcccagtctt caatttacct tcgaattgtt ggtcttctcc actcaaaata
     6661 tttaagagtg ttgttttacc agctccattt ggcccaacaa caccaatacg ctcacctttt
     6721 tgaataattt ccgtcagatg ttcaattaat actttatcat taatagcctt tgataagtct
     6781 tctaattcga acacttgctt acctaatctt gaataagcaa gattcaattc acctttatcg
     6841 tctttatatt gctggttaac ttcattttct aggtcattaa atcgattaat tctagcttgt
     6901 tgctttgtag tacgagcctt agctccagcc ctcatccaag caagttcttc cttatataaa
     6961 gctcgttgct tttgttgttg cttttgaagt gttacttctc tttcagcgcg catttcaata
     7021 taagattcat agttaccagg atatgacgct aacttacctc tgtttagttc aataattcta
     7081 gtggaaactt catttaaaaa atatcgatca tgggttacga ataaaacagt atgaggatat
     7141 tgcttcacat aattgatcaa ccagctgatt gattcgaagt ctaaatggtt cgtaggttca
     7201 tctaataaca ataaatctgg ttgttctatt aatgttttag caagtacaac acgtttttgt
     7261 tgtccgccgg ataattcttt aatgtattta gtagtatcat gtatacctag ttttgagagg
     7321 attgttttaa tttcagcgtt atagtcccaa gcatcatgtt gatccatagc atcttgcgca
     7381 tccatcattc gcttgaacaa tttatcactt tggtcatccg cataagcttg tactgcctgc
     7441 tcatattgct tgatgatgcg taaagttgtt gtatcagaac ttaatactgc atcaaaaact
     7501 gtcatatctt cattaaggtc ctgtttctga gacgaatatc gaattcgata ttgatttgga
     7561 tgcataacat tggctgtaaa atcatcatca ataccaccaa ttacttttaa caacgtactt
     7621 ttccctgtac catttatgcc tactaaacct attttttcac cttctgaaat tgataaatct
     7681 aggttatcga atatagtctt atcggcataa gatttattta aatgttcaat tttatatgct
     7741 tccataccca tcttccttct tctgttgaaa tctctacgta cttaaatcag ttctatacat
     7801 tatacacctt tttgaaatca aaagaaatcg ataccgttaa gtgtcataat ttaatatatt
     7861 gttgcttcaa ctataaataa aacttgttaa ggctttgatg actatgctaa tgatctttat
     7921 aatatacctt tttatcaaca catataatgt aaaacataat ataaaagaac ataataaaaa
     7981 cccggattga tattttatca atccgagttc gtgtttagat attatttttt agagtttgct
     8041 ttaggtcctg tttcatattt atacttcgaa ggatttacct ttttgaagtc tggatttttg
     8101 tagaatctaa acaaatcacc attaagcacg ttgtcactca tttcgagatc cttttcaact
     8161 tgctttttat tcttttcgaa atcagctggt tgagtagtta tgagttcatt atttttatta
     8221 gaataaatct taccattaac atatttataa tcttttgtta taaagtcacc atttctgaat
     8281 ggaactactt gattatgacc tttagagaat aaatcagtac cgaacattaa atagttcttc
     8341 gtatctatac cagccaaatg taaaattgtt ggcattacat cgacttgacc agcatattca
     8401 ttattgatac caccagattt accagggatt ttaatccaga aaccagttct gtttaaatct
     8461 gtaaatttag ccggtgtgat tttttcacct aatagttttt ccatggcatt gttatggttt
     8521 tcagagatac catagtggtc accataaatc ataatcactg aattgtcata taatcctttt
     8581 ttcttcaagt cattaatata ttcttctaat gcttcgtcta aataacgtgc tgtttgaata
     8641 taaccatcaa ctgttgcatc acctgtgttt gatttctcaa tagttgcatc cttttcatct
     8701 aatgtgaatg gatagtggtt agtcaatgta attaaatgag aatagaatgg tgatttcatc
     8761 ttagcttgat aattagcaga atctttaaag aaaattttgt ctttcaagcc taagtttaca
     8821 acgtttttat ctgacatgtc atagtatgtt gcatcataga atttatcgat accaaagtgt
     8881 ttatatactt ggtctctgtt ccagaatgtt ttatagtcac cgtgcatgac atcagatttg
     8941 tagccttgct tttgatctaa aattgctggt aatgactgat acgtattatc tccttttaat
     9001 gaaaaggcag aaccttgcgg taaaccgtat aaactgttat ccattgtaaa ttcagagtca
     9061 gatgttttac cttgacctgt ttgatggaaa aagttaggga agtatgtgaa ttgctctttc
     9121 ccacttgata atttgtttaa aaacggtgtt acttctttac cattaacctt tttattaatt
     9181 aagaaggttt ggaaactttc taaatgaatc ttaataatat ttttcttctt tgccacccca
     9241 taatattctg ggttaggctc tgtttgacgt tgtttcgtat aatttaatac ttttgttaag
     9301 tcatcttcag atgctagcgc tttttgttga ttattttcga tagttttaac accatcgtat
     9361 actgtaaagt tataaggtcc taaatatttc actaaatatt tatggtcaaa tgtacgtgtt
     9421 aataattctg gtctgtcagt ttcagcaaaa gctaagttta agaagaataa tgctactgaa
     9481 gccgccatta cgacaggaac aaatttctta ctaaatgctt ttgtgtctaa ccattttgtt
     9541 ttaaatatta aaatgaataa gtaaactaac gtatcaatga aataaacaaa gtcataccat
     9601 ttgaatgatg cactaaccgc accacccata gattctacgt tacctacttg gtttaaagta
     9661 ctaaacgtta aaaaatcaga gaagaatcta aagtacacaa cattggcata taataggaac
     9721 gtcaataaga agccgcctat gaacatgaac caaaatgctt ttttgccttt aaagaatagg
     9781 aacacactta aaaccagtgc tactaaacta taaggattca tcaataatat taagttttgt
     9841 actaaacctt taacacctaa agaaaaatca acataataag aaaaatacgt cttcaaggta
     9901 atcgttatta cggttaataa gaagaacgca aaaagactaa ttttcttttt ttgtgaactc
     9961 atgattcttt cccccgttat ttagataata aatcttgcga aagtacttga ataaattaga
    10021 tattaacatt atgtagttaa ttaataagtg cgtttaaaag ttaacattgt cataattata
    10081 gtaattactt tcagattcaa gtttataatt ttcgttattt gtagctttca actttgattt
    10141 aacaagtatt ttgggctaca actcatattt gcttgtgta
//
LOCUS       DALAAR010000083        10162 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000083 DALAAR010000000
VERSION     DALAAR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.085"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            163..801
                     /locus_tag="O2458_002267"
     CDS             163..801
                     /locus_tag="O2458_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCZ3888116.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            804..1727
                     /locus_tag="O2458_002268"
     CDS             804..1727
                     /locus_tag="O2458_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ3888117.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1739..3007
                     /locus_tag="O2458_002269"
     CDS             1739..3007
                     /locus_tag="O2458_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ3888118.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            3007..3630
                     /gene="udk"
                     /locus_tag="O2458_002270"
     CDS             3007..3630
                     /gene="udk"
                     /locus_tag="O2458_002270"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCZ3888119.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3658..4134
                     /gene="greA"
                     /locus_tag="O2458_002271"
     CDS             3658..4134
                     /gene="greA"
                     /locus_tag="O2458_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCZ3888120.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4460..5194
                     /gene="pxpB"
                     /locus_tag="O2458_002272"
     CDS             4460..5194
                     /gene="pxpB"
                     /locus_tag="O2458_002272"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCZ3888121.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5184..6194
                     /locus_tag="O2458_002273"
     CDS             5184..6194
                     /locus_tag="O2458_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ3888122.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6196..6645
                     /locus_tag="O2458_002274"
     CDS             6196..6645
                     /locus_tag="O2458_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCZ3888123.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6659..8020
                     /locus_tag="O2458_002275"
     CDS             6659..8020
                     /locus_tag="O2458_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ3888124.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            8020..8772
                     /gene="pxpA"
                     /locus_tag="O2458_002276"
     CDS             8020..8772
                     /gene="pxpA"
                     /locus_tag="O2458_002276"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCZ3888125.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8785..10014
                     /locus_tag="O2458_002277"
     CDS             8785..10014
                     /locus_tag="O2458_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCZ3888126.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
BASE COUNT         3633 a         1402 c         2004 g         3123 t
ORIGIN      
        1 taaattttat atgaatgatt cgcttctata aataaaatag ctttgcgtct aacacgaaat
       61 cagtcattta attggttggc aaaattataa tcaattgtga aggctgattt attttattgt
      121 cataatcata ttgacttatt ttacataaag gaagtttaaa acatggatga cctaaataaa
      181 aaatatttaa tagatttaca tcaacatcaa aatagttcaa tcgaagtttt gcgtgaattt
      241 gccgaggtaa atgaagtgcc aattgtagat cgtttaacat tagatttaat taagcaatta
      301 attcgtatga ataatgttaa aaatatttta gaaattggta cagcaatcgg ctatagttct
      361 atgcaattcg cttctatatc tgatgacatt catgtcacaa cgatagagcg taatgaaacg
      421 atgattcaat atgctaaaca aaatttagct acttatcatt ttgaaaacca ggttcgaatt
      481 attgaaggta atgctttaga gcaatttgaa aatgtaaatg acaaagttta tgatatgata
      541 tttattgatg cagcaaaagc gcaatcaaag aaattttttg aaatatatac accactttta
      601 aagcaccaag gtctcgtaat tacagataat gttttatatc acggttttgt atcggatatt
      661 gggattgtta gatcgagaaa tgtaagacaa atggttaaaa aggttcaaga ttataatgag
      721 tggttaataa agcaaccagg atatacaacg aattttttaa atatagacga tggattagcg
      781 atttcaatta aaggagaatg actatgacag aattacttgt cacacccaag tcattaagcc
      841 atatggaaac acttattgat ttaggtgcag atgcttttgt aattggcgag caaaagtttg
      901 gtttaagact tccaggggaa ttcaatagac aacaaatgac agaggctgtc gcattagctc
      961 acaaaaacga taaaaaagta tatgcggcag tgaatggatt atttcataat tatcatttag
     1021 atgctgtaga agattatatt aattttttac atgaaatacg agttgacaga attattttcg
     1081 gggatccagc agtagtaatg tatgtgaagg cgcaagaaaa tccaatacca cttcattgga
     1141 atgcagagac gttagtaact aatcattttc aatgtaatta ctggggtaaa agaggcgcat
     1201 caagagctgt cttagcacgt gagctgaatt tggatgaaat tattaatata aaagaaaatt
     1261 caaatgtgga aattgaggtg caagttcaag gaatgacttg tatgtttcaa tcaaagcgta
     1321 tgctactagg caattattat acgttccaag atcgccaaat gaaaattgaa cgtcgcaatg
     1381 atgaacaatc tttattactt tatgatgaag aaagacaaaa taattaccca gtttacgaag
     1441 attacaatgg tacgcacatt atgtcaccaa acgacatttg tttaattgaa gaattagcac
     1501 cattttttga ggcgggtata gattcattta aaatcgatgg tattctacaa acggaagaat
     1561 atattaatgt ggtaacggaa cagtatcgtc aagcgataga tttgtacaat gaagatcctg
     1621 aaatctatga agatgagaaa tttatgttga tggatccaat tgaagaaatt caacctgatc
     1681 atcgaccatt tgacgaaggt ttcttatata aacaaacagt atattaagga ggttaatcat
     1741 gaagacaata gaagagatta aatcaactcc taaaacagtt atgaagaaac cagaattatt
     1801 agcacctgct ggaaacttag aaaagttaaa aatagcagta cattatggcg ctgatgccgt
     1861 atttttaggt ggtcaagaat atggattacg ttcaaatgct gataatttca cgatggaaga
     1921 aatagctgaa ggtgttgaat ttgcgaaccg ttacggtgcc aaaatttatg ttacgacaaa
     1981 tattattgca catgatgaga atattgaagg tctagaatca tatttgcgta atttggaaaa
     2041 gactggtgcg acaggtatca ttgttgcaga tcctttaatt atcgaaacat gtaaagaagt
     2101 tgcgccaaaa cttgaaattc atttatctac tcaacaatca ctttctaatt acaaagctgt
     2161 agaatattgg aaagaagaag gattggatcg tgttgtatta gcacgtgaga ccggcgcgat
     2221 ggaaatgcgt gaaatgaagg aaaaagtaga tattgaaatc gaagcattta ttcatggtgc
     2281 tatgtgtatc gcctattcag gtagatgtac attaagtaat catatgactg caagggattc
     2341 caacagaggc ggttgctgtc aaagttgccg ttgggattat gaattattag aagttgatga
     2401 taatggtgaa cttgatgttt tttataatca aggtgaagtt acaccgtttg cgatgagtcc
     2461 taaagattta aaattaatcg aatcaattcc tcaaatgatg gatattggtg tggactcatt
     2521 aaaaattgaa ggacgtatga agtcaattca ttatattgca acagttgtct cagtatatcg
     2581 taaagtcatt gatgcgtatg cggcagatcc tgacaacttt aagattaatc cggaatggtt
     2641 aatagagtta gataaatgtg caaatagaga cactgcacca gcattctttg agggaacacc
     2701 tggttatgaa gaacagatgt ttggtcaaca acaatctaaa aaatcaccat ttgatttttg
     2761 tggtttggta ttagactata atgaagatac aaaaattgcg actattcaac aacgaaataa
     2821 ctttaaacca ggtcaagaaa tagaattctt tggtccagaa attgaaacat ttacacaagt
     2881 agtagaagca atttatgatg aagaaggtaa cagcttagac gcggcgcgcc atccattaca
     2941 aatcgttcaa attaaagtgg atcgcccgat atatccaaac aacatgatga gaaaggaaat
     3001 tggctaatga aagctactac aatcattggc atagctggtg gatctggctc aggaaaaaca
     3061 actgtaacta acgaaattat gaaaaactta gaaggtcata gtgtcgcttt acttgctcaa
     3121 gattactatt ataaagatca aaagcacttg actttcgacg agcgcctaga aaccaattat
     3181 gaccatccat ttgcattcga taatgattta ttaattgaaa atcttaaaga cttgaaaaat
     3241 ggtaaagcag tagaagtacc gacatatgat tatgctagtc atacaagaag tgacattacc
     3301 attgatttta aacctaaaga tgttattatc gtagaaggca ttttcgcttt agaaaataag
     3361 gtattacgtg atatgatgga tgttaaaata tatgttgata cagatgcaga cttgagaata
     3421 ttacgccgtt taacacgaga tactaaagag cgtgggcgtt caatggactc tgttatcaat
     3481 caatatttaa gtgttgttag acctatgcat gaccaattta ttgaaccgac taagaaatat
     3541 gctgatataa ttattcctga aggtgggagc aataaagttg caatagatat tatgacaaca
     3601 aaaattcagt ctttagttag caagcaatag tataattcag taaaggaaga tgacaatatg
     3661 gaaaatcaaa agcaatatcc aatgactcaa gaaggttttg aaaaattaga gcgtgaactt
     3721 gaagaattaa aaacagttaa gcgtcctgaa gttgtagaga aaattaaagt tgcacgttca
     3781 tttggtgact tatcagagaa ctctgagtat gatgcagcaa aagatgaaca aggattcatc
     3841 gaacaagata ttcaaagaat tgagcatatg ttaagaaatg cattaatcat tgaagatact
     3901 ggagataaca acgttgttaa aattggtaaa acagtaacgt ttgtagaatt accaggtgat
     3961 gaagaggaaa gttatcaaat cgttggttca gctgaatcag atgcatttaa tggtaagatt
     4021 tcaaatgaat caccaatggc taaagcgtta attggtaaag gtttagatga tgaagttcgt
     4081 gttccactac ctaatggtgg cgaaatgaac gtaaaaattg ttaatatcca ataaatgatc
     4141 aaatagatta tttaattgcg cgattgaagt gtgttaatga tacttcggtc gtgctttttt
     4201 atattcaaaa tcaaaaaatc ggacagatga aaagtaaaaa ttttccatca gaccgaatga
     4261 tagcagaacc taaattagtg taagagttac aatattaact actccatttt aaaaaagcaa
     4321 aatatttccg agaaaacaaa ttgattatca tttgttattg tatgcgattt gaattactta
     4381 ttaaacaact tgtgcttctc aatgatacaa tgaaaaggta ttcaatactg gtttattgca
     4441 atacaaaggg ggatttaaag tggatgtaag atttattaac gagcagacaa ttatgattta
     4501 ttttgaaaat aaaatatccg aagagacata tcgtaatgtc acagcaatgg tacggtggat
     4561 acgagaaaaa gaaatattag aaattcaaga tattgtccct tcgtatcgag cagttctaat
     4621 atattttgat gaacaagcta taacatcttc taaattaatt gaaaatttag aacttaacaa
     4681 atttaatgaa aaaaatgttc atgctgtgaa tcaaaccaat cgtattatta aaatacctgt
     4741 tcaatatggt ggtacatatg gaccagatat tgaagaagta gcaaagcata atcgaataac
     4801 tgttgagcaa gttattgaaa aacatacaag taaaccttat ttaatatata tgctaggatt
     4861 tatgccagga tttccatact taggcggact tgatgaacaa ctacatacac ctagacgtaa
     4921 tcagccaagg ttaaaaattc atgcaggttc tgtaggtatt gcgaataatc aaacaggact
     4981 atatccatca gattctcctg gcggatggca aattattgga cgcacaccgt taaaagtatt
     5041 tagcagcgag cgtgaaccaa tgtcaatgta cgaagcagga gaatggatac aattttatgc
     5101 aatagatgaa cagaaattta tacaaatcga aagagatata tcagatggca actttaatgt
     5161 tgatgactgg gtggtgattg aaaatgtcaa ttaagatttt acaaccaggt ctcttttcaa
     5221 cggtacaaga tctaggaaga ataggttatg aacatattgg attttcaggc gcaggtgcca
     5281 tggatcaatt tagttttaag gttgcgcagt cattaattaa caatgatggt ccagcgattg
     5341 aatatacttt gattggtcct accattcaat ttaatacgca aaatacattt gttataaccg
     5401 gtggtagtat taatgcttcg ctaaataata aaactatatc aatgaattct gtcatattag
     5461 ctgagaaagg tgacatttta aaaataggtg ctataaccaa aggtgcacgc ggttatctta
     5521 cttttggtca ttctatcaac gtaccttcta ttgctgaaag ttatgcaaca catacccgaa
     5581 gcagtatagg aggattcaaa ggtagaaagt tattagctga tgatgtaata acagtgaaaa
     5641 taaataatga ctttaaagaa aacattggaa agacgattca tttacaggac gatttattgc
     5701 cagaaaataa tattatacac attcttcaag gacctcaatt cgaggcattt tctgaagagg
     5761 ctagagcgaa aattgtaaat catccatatt taattactga acaatcagac cgtatgggct
     5821 atcgtttgga aggtgacagc gttgcaccaa ttaatcaagc agatatcatt tctgaaccgg
     5881 ttgctcttgg tagtgttcag gtaccaaatg atggtcaacc tattattctg cttaatgata
     5941 aacaaacgat aggtggttat accaaaattg caactgtatg caaatttgat ctgccaaagt
     6001 tagcacagat gaaaccacaa gatacaattc aatttaaatg gatatcattc caagaagctg
     6061 tagataagaa tcgggaacaa atgtctctgt ttaatgaaat tcttaagagt catcaaaaga
     6121 ctccaatttt tgatacatct agcttgcgac atacttcgaa aaaattagca acaattttaa
     6181 aaggggattt gtaaaatgaa tattgaaaaa atcgaacaaa taatcaaatt agtgaaggaa
     6241 aatgatgtta agaaatttaa atataaaaat tttgaagatg aaatagaaat tgacttcact
     6301 gactcgaatc atttggctgc acacagtaat caatcaaatc aaagtatgaa caataatgat
     6361 ttgacagctt caaaagcgaa tgataactcc gatgtttcga caaatgatta tcatgacatt
     6421 aaatcaccaa tgataggtac attcttttta caagatagta aagaattaac tgaaccaatt
     6481 gtgaatgtcg gtgacaaagt taacaaggga gatattatag gatatgttga agcgatgaaa
     6541 gtattaaacg aggtaacaac agatgttgct ggagaaatta ctgaaatagt agctgatcat
     6601 ggcacaaatg ttgaatacga ccaagttttg gtacgtatta agtaaagggg gtaagtaaat
     6661 gcttcgttgt ttaattgcga acagaggtga aattgctgta aggattataa gagcttgcag
     6721 agaatatggg attgaaactg tagcagttta tgcaaaaggg gacgagcaaa gcttgcatgt
     6781 acatttagca gatcaagcta tatgtattgg ggaagctaat gctttagata gttatttaaa
     6841 tattgaccgc atcatatctg ctgcacaaat cactggtgct aatgcaattc acccaggata
     6901 tggcttttta tcagaatcaa caaaatttgc tcaaaccgtt gaggaacaag gcatagcgtt
     6961 tatagggcct actaaaaaaa cgatggaaat gatgggggat aaaataacag caagacaaac
     7021 agttcatcat gcaggtgttc ctgtgattcc aggatcaaat ggtgctgtga atcatgtgtc
     7081 tgaaattgag aatcttgcca aagacatcgg ttatccagtt gttatcaaag ctgccagtgg
     7141 cggtggtggt aaaggtatac gtattgtaaa gaaagctgaa gatttagaaa aggcatttaa
     7201 agaagctaaa agtgaaggaa aaaaatactt tgatgatgat cgtgtttatg ttgaggcttt
     7261 tatacctgtt gcaaaacatg ttgaagttca agttatggga gatgggcagg ataattatgt
     7321 acatttaggg gaacgtgatt gttctgtaca acgtaaaaat caaaaattaa tcgaagaatc
     7381 tccttgtgca gcattaactg aagaaagacg acaacaaata tgtaacgatg ctgttaaagt
     7441 tgcacgtgca gcaaattata gaagtgcagg tacgattgaa tttttagtaa ctgataccgc
     7501 acattacttt attgaaatga atgctcgaat acaagttgaa catactgtta cagagatgcg
     7561 agcagaacgt gatttggttg cagcacaact gtatttattg gaacataatc atttaccttt
     7621 ttcacaaagc gatattcaat ttaatggtca tgtgatagaa gcgagaataa atgccgaaaa
     7681 tcctgaaaag aaatttcaac caacaccagg taaagtgaca gcgttacatt tacctcaagg
     7741 ctttaatgtg cgagtcgatt ctttactata tcacggttat caagtttcac catattatga
     7801 ttctttagta gctaaagtaa ttgtaaaatc tcatgataga gcttcagcaa ttgataagtt
     7861 gaaagtcacc ttagatgaaa tggtgataga tggattttct acaactgccg attttttata
     7921 tgcagtttta aattatccac tatatcgtga tggagatgca aaagatgttg atattaaatt
     7981 tttagaaaaa catcaaattg ttaaaggggt ggaattataa tgcgagttga tttgaattgt
     8041 gatttaggcg aagcatttgg aaattattcc tttggtggtg atcatcaaat tattccgtta
     8101 attacaagtg cgaatgttgc ttgtggtttt cacgctggtg atgaaaatgt aatgaatgaa
     8161 acggtaaaac ttgccaaagc acataatgtt gcagtaggtg cacatcctgg tttacctgat
     8221 ttgaaaggct ttggcagacg aaatatagat atctctaacg acgagattta taatttgatg
     8281 atttatcaat taggtgcatt acaagggttt tgtcgcattc atcaagttaa aattaatcat
     8341 gttaaaccgc atggtgcatt gtatcagatg ggtgcaaaag acagagaaat agcaaacgtt
     8401 atagcacaag ctgtttatga ctttgatcca tcactagtgt tagtaggatt agcaaattca
     8461 tatctaattt cagaagcaaa gaatgtcgga ttaattacag cttctgaagt gtttgctgat
     8521 agacgatacg aagatgatgg gcagctcgtt agtagaaaag aaagtgatgc tgtgattact
     8581 gatactgacg aagcacttaa gcaggtttta aagatggtga aggaaaataa agttatttca
     8641 aaaaacaata aggaagtaac gttacaagca gatacaattt gtgtgcatgg tgatggagaa
     8701 catgcattat tatttgtttc gaaaattaga gaaattttaa tgaaagaagg cattgatatt
     8761 caatccttat agggggctaa aaaaatgggg agaaatttaa agcttaaaaa agaaagtgac
     8821 tttgaattca caaaaaatca taaaaggtta ttattaggtt ctgtattttt gatggcaact
     8881 tctgcaattg ggccagcatt tttaacgcaa acagcagtat ttacatcaca attttttgca
     8941 agtttcgcat ttgccatatt actgtctatc atcattgaca ttggtgcaca aattaatata
     9001 tggcgcatat tagttgtaac tggtttaaga ggtcaagaaa tatcaaataa agttgttcct
     9061 gggcttggta ctgttatctc aatattgatt gcatttggtg gtctagcttt taatattggt
     9121 aatattgctg gtgccggttt aggtttaaat gcaatttttg gattagatgt aaaatggggc
     9181 gcagctatta ctgcaatctt tgcaatatta atctttgtaa gtaaaagtgg ccaaaaaatt
     9241 atggacgttg tttcaatgat tcttggtatt gtgatgattt tagttgtggc atatgtgatg
     9301 tttgtttcta atccacctta tggtgatgct tttgtgcata catttgcgcc agaacatcca
     9361 atgaaattag tcttgcccat cattacgtta gttggtggaa ctgtaggtgg ttatattacc
     9421 tttgcaggtg cacatcgtat attagactct ggcattaaag gtaagcaata tttaccattt
     9481 gtaaatcaat cagcaattgc tggtatttta actacaggta ttatgagaac gttactattc
     9541 ctagcggtat taggagttgt tgtaacaggt gtgacactaa gttctgaaaa tccaccagcg
     9601 tcagtttttg aacacgcaat tggaccaatt ggaaagaata tttttggtat tgtgttattt
     9661 gctgcagcta tgtcatcagt aattggctca gcatacacaa gcgcaacatt tttaaaaaca
     9721 cttcataaat cacttaacga aagaagtaat ttaattgtga ttgtgtttat cgttatttca
     9781 acaatgattt tcttatttat tggaaaacca atcagccttt taattatagc aggcgcgata
     9841 aatggatgga ttttacctat tacgttaggt gcaattttga ttgcaagtaa aaagaaatca
     9901 attgttggtg attataaaca cccaaattgg atgttcatct ttggtatcgt tgcagtactt
     9961 gttacaatat tgacaggtat attctcattc aaagaagtac ttcagttatt ttaaaagaca
    10021 tttcgactaa tagcatcact gtaaatgttg ttagtattag gcaagatata ttttgatttc
    10081 cggtgattca tttataaagc ataattagat atattttaag ggagagtggt gcatgacaaa
    10141 tgtactgctc tttttgttgt ga
//
LOCUS       DALAAR010000084        10074 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000084 DALAAR010000000
VERSION     DALAAR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.086"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            3..1310
                     /locus_tag="O2458_002278"
     CDS             3..1310
                     /locus_tag="O2458_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ3888127.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(2163..2606)
                     /locus_tag="O2458_002279"
     CDS             complement(2163..2606)
                     /locus_tag="O2458_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888128.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(2771..3148)
                     /locus_tag="O2458_002280"
     CDS             complement(2771..3148)
                     /locus_tag="O2458_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888129.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(3466..4056)
                     /locus_tag="O2458_002281"
     CDS             complement(3466..4056)
                     /locus_tag="O2458_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCZ3888130.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(4053..4265)
                     /locus_tag="O2458_002282"
     CDS             complement(4053..4265)
                     /locus_tag="O2458_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCZ3888131.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            4326..4872
                     /locus_tag="O2458_002283"
                     /pseudo
     CDS             4326..4872
                     /locus_tag="O2458_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4967..6583)
                     /gene="groL"
                     /locus_tag="O2458_002284"
     CDS             complement(4967..6583)
                     /gene="groL"
                     /locus_tag="O2458_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCZ3888132.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(6659..6943)
                     /gene="groES"
                     /locus_tag="O2458_002285"
     CDS             complement(6659..6943)
                     /gene="groES"
                     /locus_tag="O2458_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCZ3888133.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            7118..7861
                     /locus_tag="O2458_002286"
     CDS             7118..7861
                     /locus_tag="O2458_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ3888134.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(7886..9139)
                     /locus_tag="O2458_002287"
     CDS             complement(7886..9139)
                     /locus_tag="O2458_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888135.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            9336..9962
                     /locus_tag="O2458_002288"
     CDS             9336..9962
                     /locus_tag="O2458_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCZ3888136.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT         3242 a         1787 c         1393 g         3652 t
ORIGIN      
        1 aaatgaacaa agtccacaaa cctttatatt tttatttaat gcttttcttc tctacaacta
       61 tcataggtgc acttttattg tatttacctt ttactggtaa aaagccaata tcttttttag
      121 atgccctatt tatagcttca agtgcattta cagttactgg cttgtcccca gttgatatag
      181 gatcacagtt taatatactt ggtgagatag taatactatt attaattcaa ataggtggtc
      241 tgggtatcgt gaccgtaacc ctattgacac tagtattttt aaatagaaag atatcaatga
      301 aaaatagatt cttgattatg gttacatgga atattgacga acctggtggt gttattaagc
      361 taattaaaca cttggctatt tatagtttag tcactgaatt aattggtatg atttgtttgt
      421 gtttatcttt tataccaaaa tttggtatag gcaaaggttt atttttaagc ttattcacat
      481 cagtatcagc ttttaataat gctggatttg ccctttttaa gaataactta atagattatt
      541 ctagtgatcc aattgtcatt attacaatct caatactcat aatatttgga ggtattggac
      601 attttgtcgt gatagacttt attaattgta aaaaattgag taaattatct ttacattcta
      661 aattagtctt aactacaact agtatcctaa taattatagg agctattaca ttctttttat
      721 tagaacagtt taatactatg caacatatgg gactagttga aaaaatcgga aattcttttt
      781 tccaatcagt aacaacacga acagcgggtt ttaacagtat agatatagca agcattaaca
      841 aatctaccgc attaatgtta atgctactta tgtttattgg tggtgcccct ctcagtgcag
      901 ctggaggaat taaaataact acttttgcag ttgcgtttat ttttgtacta aattatatac
      961 gtaaagaaaa taatgtttca gtattcaata aagaaatatc tgacaaacat ataaaactat
     1021 ctattgttac cattaatatc tcatttctat ttatcagcat cattactttt atattatcga
     1081 taattaatcc gaacatatca ttaatcaagt tattattcga agtggtttct gcattcggaa
     1141 cagtagggtt aagtatgaac cttaccacag aatatcatgg tattactaaa ataattatta
     1201 tattcgttat gctttgcggt aaagtaggac tattaacttt attaagaaca tttataccac
     1261 caaaaagtcc taaaaattac cgctacacta aaggacaaat ttatctataa aataactatc
     1321 catctttaat aagaaatgaa ctagtgtttt aatctattac attaaaaaaa ctacctaaac
     1381 aggtagtttt ttatttattt taggccgatt aaatgtctta tgtctttaat taagtaaaat
     1441 gcacaagtac acctttaaaa agatttactc aaaactaaac tatacctagt taaccgcttg
     1501 tgattaagaa attttaaatg tggtttcttt taattatgtc aaccacccat aaggatttta
     1561 atcaagcact cattcattcc tgcactcgca atatgcatta cttatagaag tggatgcact
     1621 ctcatattat ggcaatacca taactcccgc accataatga tttaaatacc attatgaatc
     1681 tcaattacaa attagcacat aaaaaagacg cctcccataa gtctagtcac caaatggaaa
     1741 acgtctcgta attaaaatat atttgcgtgc attctaattt gtacttagaa tacatcatac
     1801 tcgttcataa tgtaattttc taattagtca aaactatata cagttttaca tcattcccgg
     1861 cattatgcac taataaaaac ttactttaat acacgcttta aagattatta aatcagcgtt
     1921 ttaattttta gacaacgcta ttatttttag tttttcaatt ctattatgtc ataattatgt
     1981 cactcaaaaa ctgttttcca acattgattt attgttggaa aatctcgaaa atctagtcta
     2041 tttctccaac aatcgaagaa ttatgccctt ttttctccct ttaaataagt cataatacga
     2101 ggcatacatg caacatttac aataaaatat gttcctacat cgtattatac gaatgctcac
     2161 acttatagtt gtttagtaaa cccagatgta ttgctaacat acccattatc actatatagt
     2221 ttatgtgcag atagtctttc atttctatta ccactattta gtgttattgc tttacaattc
     2281 aaccgtttag aaaattcttc agaatcagct aataatctct ttccataacc ttttttccta
     2341 aattcagaat gtataacaaa cgcaaggatt ctcatatact ctgcattttt ttcgtaaaac
     2401 atcattttac acataccact taaaccaatt attttatttt cttttatcaa aagcagtagg
     2461 aagtaatcat catgattcgt tattttcttt agtctctttt ttaaatcatt ctcatttgtt
     2521 ggataaccta gatcatcata taatttacat agttgattta atttttcaaa atcactgtta
     2581 ttaaacaatc ttgtaattat gctcatataa cccaccctaa aaatattatg tacctattgt
     2641 tacatacaat agatacgcaa aatcatttct cactgcaaat caggacgttt ctcagcgtaa
     2701 acaaatcatt tctattcttt tgaaaaatat ctgcatatct tcacagtaat tttataacag
     2761 attaataaaa ttaaaaaaac ataacaacaa gtattataat aaatattata atagctatcg
     2821 tattaaatag tttagaatat gcaaactgaa aaataaaaat cacaattaaa atactaagta
     2881 atgtaaatgg taatagcatc atcagatctt taacattatt tctttgttct tgctcccaat
     2941 cgctaatagc cctaaaacta ctttttttat ctataggcga tgtaaaaata ccatatattg
     3001 aaggtgctat acctcctaaa atagcagttc ccaaagttgt cattactgaa attactgcga
     3061 aagtatcatc cgaaagcaat aaattcaaac taatgcattg tttattaccc atcgaattta
     3121 ttgaccaaat agctagagaa ataaacaacc caaaatttaa aataaatgat atagtaatag
     3181 caattgttta caaaacacga aatttttcat ttttatttat attatccatt tttctccctt
     3241 tttcttaaat cattttatta tatattacaa taatcaatct gaaatgttga tgtaatttga
     3301 aaaaaatatc atacttttac tcctgaaaac ctccctaaat catcaatatg aaaatcagta
     3361 ttaggatatt gcgtttacaa ctcttttaaa tctcactcat tcttctcatc gtcttaaccg
     3421 atactatcaa taaatactgg caacaccata aaatatatac ccaatttata aaaacctaat
     3481 tgactcataa tcttctaacg gtatatcatc cacaatcaca gtatgattag gattatcgtt
     3541 agatacatct ttcactgcct tatctaaccc ctcatcacct ccgtcccatt caccaatatt
     3601 aatgaatata ggtacattac cgtttatatc atgcttatct gtaaataatt tatgatattt
     3661 acccaacata tcacgagctt ttaaacgatc actaggtttt attggtacct ctatcagttc
     3721 aacatgttca ttatagacta attgtacttt gccactttgt ggattttctt tatattctcc
     3781 acgcttgact acaacttctt tcgtttctgt ttcatcaccg actgccgcat tcgttagcac
     3841 atgtaataac tcttttgcgg ttaatacatt ctcatctata atcttatctt tttgttcttg
     3901 tatatattgc ttgatgtgcg gctttttcaa taacctacac gctgtcacat gtgcgctatt
     3961 tgcgttatat cctgctttta tggcactttg tgttacattc agtgttctaa tatactcatt
     4021 cacaaaacgt gcttgctttg cagttaactc actcatttta tcacccccac aattttatct
     4081 aatatggttt cataccataa tattacagat tgttctgaac aatctaaggc actactaata
     4141 tcttgataac taagtccttg tataagggag tcaaaaatat aaaactcttt atcggtcgct
     4201 aatctgtcaa caatcatttc tatgtgattc tttataatat gatcattgac attatcgtct
     4261 gtcatcaatt cgtcagaatc ttcatcacct attaaaaaga aatcatcagt atttatttca
     4321 tcatcgcccc gttaactagc tttgaagtct ttagcacact tgcatatacc ggctgtcgtg
     4381 ctggcgagat actagcattg aagtggtctg atattgattt tgaaaacaac acgattagta
     4441 ttactaaaac atattacaat ccaaataata acaagaagaa atatcagata cttcccccta
     4501 aaactgaaag ttctatcggt aagatttccg ttgacccaaa tgtaataaag gtgttgcgtg
     4561 attataagat aaacgttcaa aataattgga aaaacgaatt atataatgat aaccattgta
     4621 atgaaaaaac tatcaatgtg gatacaaacc attatgtcac gtaccagcat actcaaaaat
     4681 atctctacgc actcattcag atacacacat tgcgcattgc tcatagaatc aggtgtacac
     4741 attaaagaaa tacaagaacg attacggcat aaagatatca ataccactat gaatatctat
     4801 gctaaaatca caaattcata caaaaaagac gcctcccata agtttagtca ccaaatggaa
     4861 gacgtctcgt aattaaaata tatttgcgtg cattctaatt tatacttaga atgaatcata
     4921 ctcgtgcata atgtaatttt ctagttagtc aaaactataa acagttttac atcattcctg
     4981 gcatgccacc catgttaggt tggtcattat ttttttctgg aattgatgct acaaccgctt
     5041 cagtcgttaa gaacattgct gcaacacttg cagcatgttg taatgctgag cgtgttactt
     5101 tagttggatc aacgatacct tcttctaaca tattaaccca ctcgtttgta gcagcgttaa
     5161 aaccaacacc cggctctgcg tttttcaaac gttctacaat aacagaacct tctaatcctg
     5221 cattttcagc aatttgacga actggtgcag ttaatgcttt aagtacaata tttacacctg
     5281 tttcaatgtc accttcagct tcaatttcac ttactttttg gtaaacattt actaatgcag
     5341 taccaccacc tgcaacaata ccttcttcaa ctgctgcacg tgtagaattt aatgcatctt
     5401 caatacgtaa tttacgttct ttaagctctg tttcacttgc tgcacctact ttgataactg
     5461 caacaccacc tgctaattta gctaagcgct cttgtaattt ttcacgatca aagtcagatt
     5521 cagtttcttc aatttgagat ttcaattggc taacacgtgc atcaatgctg ttttcgtcac
     5581 cgtcaccatc aacaacagtg gtattatctt tagttacttc tactttactt gcagtaccta
     5641 acatatcaat tgatgcatct tttaaatcta agcctaaatc atcagtaatc acttgcgcac
     5701 cagttaaaat agctaaatct tcaagcatcg cttttctacg atcaccaaaa ccaggtgctt
     5761 ttactgcaac agctgtaaat gtgccacgca tacggtttag cacgatattt gttaatgcat
     5821 cgccttcaac ttcatcagct acaattaaga ttggacgatt agattgaacc acttgttcta
     5881 ataaaggtaa gatatcttgg aaagacgaga ttttcttatc tgttactaaa atgtatgggc
     5941 gttctaattc agcaaccatt ttatctgaat cagtaaccat atacggtgat tgataaccac
     6001 gatcaaattg cataccttca accacttcta gttcagtgtt tagtccattt gattcttcaa
     6061 ttgtaatgac accatcgtta cctacttttt ccatagcttc agaaatataa cgtccaattt
     6121 cttcatctgc tgctgaaatc gcacctactt gcgcaatttc atttttattt tcaacttttt
     6181 gagaattttc atgtaacgct tcaacagcaa ctttaactgc tttgtcgata ccttgtcgta
     6241 aaccaactgg gttcgcacca cttgtaacat ttttcaagcc ttcttgaatc attgcttgag
     6301 ctaatactgt tgcagttgtc gtaccgtcac cagcaatttc atttgtctta tttgcgactt
     6361 cttgaactag tttagccccc atattttcat atggatcttc taattcgatt tctttagcaa
     6421 tcgtcacacc atcattcgta attaaaggtg ctgtaaactc tttatctaat acaacattac
     6481 gtcctttagg accaatcgtt actttaactg catttgcaag ttggtcaaca ccacgtaaca
     6541 ttgcttgacg tgcatcttca gagaatttca attgtttaac cataaatgat aaacctccat
     6601 ttgtttagtt gtgtttcatt ttcgttctat ttaatttatt tatgaattaa gttctgtatt
     6661 attcaataac tgctaaaata tcttcttcat ttaataccag atatgtttca ttatctcgtt
     6721 taacttctgt accagcatat tgttggaaca cgacacggtc cccttctttc acttcaggag
     6781 tcactcttgt accatcattt aataggcgtc cagttcctac tgcaacgata acgccttcgt
     6841 ttgatttttc tttagcacta tcagttaaaa caataccact tttagttgtt tgttcttgtt
     6901 cttttttctc aataatcaca cgatttccaa ttggttttag catgattgtt cctccttaaa
     6961 aaacctaaag tttagcactt aacattaaag agtgctaaca tacatttata ataatcaaat
     7021 ttggtcaaaa tttcaagtca taacctttta attaagtttt gtaacttcaa gttatttacg
     7081 ataaaataaa ttataaacaa atatttggag gaaaattatg acaagattat gggcatcatt
     7141 gctaactgtt attatttata tattgtctca atttttaccg cttctcattg taaaaaaatt
     7201 accatttgta caatatagtg gcatagaact gactaaagca gtcatttaca tacaacttgt
     7261 tctattttta atcgccgcca cgacgattat tttaattaat ttaaaaatta aaaatccaac
     7321 aaaattagaa ttagaagtta aagaacctaa aaaatatatc attccatggg cattgcttgg
     7381 atttgcattg gtaatgattt atcaaatggt agtgagcatt gtattaacgc aaatttatgg
     7441 tggacaacaa gtaagtccta atacagaaaa gctaattatt attgctcgaa aaatacctat
     7501 atttatcttc tttgtatcta ttattggtcc tttattagaa gaatatgtat tcagaaaagt
     7561 aatctttgga gaattattta atgcgattaa aggtaatcgt atcgtggcat ttattattgc
     7621 tacaacagta agttcattaa tatttgcatt agcacataat gatttcaaat ttattccagt
     7681 ttattttggt atgggtgtca ttttttcatt agcatatgtt tggacaaaac ggcttgctgt
     7741 tccaattatt atccatatgt tacaaaacgg atttgtcgtt atattccaat tacttaatcc
     7801 tgaggctttg aaaaaagcca cggaacaagc gaattttata tatcacattt ttattccata
     7861 ataaaaaaaa cgaatcacac acggattatc gtcgctgtga ttcgttttta tttttctttt
     7921 tatttcgctt tacaaataag aatagcatga ttgaaaatac caccatcata gaacatacaa
     7981 ttgctagcca acttttacta tatgcagttg acgcactttt cttattatct tcttgttttt
     8041 taactactgt aaaatcgtct ttttcatgcg gttcaaatga gcgttgtttt gaatccggct
     8101 tatcttcggg ctttttcaaa tctggcattg atccaatttc accagtaatt aatgcgagta
     8161 cttgttcatc taaattacga tagtaccttt ctgatttcaa tggattgaaa tattggaatc
     8221 taaagtaatt cgattgctgt tgtaatccat ttaaatatgc atttccgctg aaattttgtt
     8281 ttcgaataat attataaatg tcttcatcag taacttctcc ataatctttg cccaacttat
     8341 taatttgatt taaaatatac ggattatact tgtaagcccc atttgccaag gctaaaaatc
     8401 gttgggatac aaaatcatta ccagtaggat tttgtgagtt tccttgattc ccatttggtt
     8461 gccattgacc ttgattagat ccatctgaag catttgggtt ccatgtcccc ccatttttcg
     8521 agccaccaga atgattggaa tccccaggtt gatcgggatc tggtgacggg ttaggatttg
     8581 gcttattagg gtctggtttt ggattcggat ttggctttgg tttatctgga tctggttttg
     8641 gatccggatt tggcttatta gggtctggtt ttggatctgg atttggtttc gggttatctg
     8701 gatctggttt tggatccgga tttggctttg gtttatctgg gtctggtttt ggatccggtt
     8761 ttggtttcgg gttatctgga tctggtttag gattcggact tgggttttgg ttatttgggt
     8821 ctggttttga atctgtactt gggttttggt tgtttgagtc tggcttcaca ttattcgaat
     8881 caggattagc atcacgacta ttcgaacctg ttccttcatt acccgtttga tttgattcgt
     8941 gaggtgtccc agtctcatta ttattcattt cactatttac tgatggtgaa gttatattat
     9001 gattcaaatt agtagttggt ttttctccat ttaagttatc ctttgcttca actgtgtgcg
     9061 ttgcaagccc taaactacta ctacttaaaa ttaaaatact tgttgaaagt aacattttct
     9121 taaaccaatg atatgacatt tcacgagaca cctccctttt tcatgtaagt attaatctaa
     9181 gtataataaa tatgcgttga aaatgcattt atatttggaa tttgaaaatg aatattaata
     9241 ttcttgaaat tttcgtaaat attgctttac aagtacgagt ataaatttta tacttagtct
     9301 attaagtatt ctttactaaa gaaggagcaa taattatggg cttatttaaa aaagataaaa
     9361 aagcaatgac ttttgacaat gcaatggaag aaagacgttc aatatataat ttgaaagatt
     9421 cgatatcaat tagcgatgac gaattagaat caatgattgc acatgcagtt aaacatgttc
     9481 cttcatcatt taattctcaa tcaactagaa ttgttctctt gttaaatgac aacaataata
     9541 aattttggga taacacaaaa gcaattttaa aagaagtcat gggagagaat cgtgatttcg
     9601 agccaactga acaaaaaatt gataacttca aacattcata cggtacaatt ttgttttatg
     9661 aagatcaaga tgttgtaagt ggtttacaag aacaaatgcc aaactactat gataattttg
     9721 caatatggtc aactcaaaca aatgccatgc atcaatttgc aatttggact gcattagcta
     9781 ctaaaggcat aggtgcttca ttacaacact ataatccact tgttgatgag atgacttcaa
     9841 atgaatttaa tattcctaaa agttggaaac ttattgctca aatgccattt ggtgatattc
     9901 gtgaagctgc tggcgaaaaa acatttaatc cagtggaaga tcgtttcgta ataaaaaaat
     9961 aatcaatata ttgttaggaa tgggacagaa atgataaaga atcattagtt attcattatg
    10021 tagtagttct tacacattag ccgctgctaa tgtgcactta agaataggaa taca
//
LOCUS       DALAAR010000085        10020 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000085 DALAAR010000000
VERSION     DALAAR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.087"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(290..1168)
                     /locus_tag="O2458_002289"
     CDS             complement(290..1168)
                     /locus_tag="O2458_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCZ3888137.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1347..1727)
                     /gene="gcvH"
                     /locus_tag="O2458_002290"
     CDS             complement(1347..1727)
                     /gene="gcvH"
                     /locus_tag="O2458_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCZ3888138.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(1885..2241)
                     /locus_tag="O2458_002291"
     CDS             complement(1885..2241)
                     /locus_tag="O2458_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCZ3888139.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            2385..2705
                     /locus_tag="O2458_002292"
     CDS             2385..2705
                     /locus_tag="O2458_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ3888140.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(2855..3394)
                     /locus_tag="O2458_002293"
     CDS             complement(2855..3394)
                     /locus_tag="O2458_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCZ3888141.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(3477..4193)
                     /locus_tag="O2458_002294"
     CDS             complement(3477..4193)
                     /locus_tag="O2458_002294"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCZ3888142.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4341..4763
                     /locus_tag="O2458_002295"
     CDS             4341..4763
                     /locus_tag="O2458_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCZ3888143.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            5162..5656
                     /locus_tag="O2458_002296"
     CDS             5162..5656
                     /locus_tag="O2458_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888144.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            5811..6428
                     /locus_tag="O2458_002297"
     CDS             5811..6428
                     /locus_tag="O2458_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCZ3888145.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(6501..7082)
                     /locus_tag="O2458_002298"
     CDS             complement(6501..7082)
                     /locus_tag="O2458_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ3888146.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            7307..7543
                     /locus_tag="O2458_002299"
     CDS             7307..7543
                     /locus_tag="O2458_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888147.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(7540..7743)
                     /locus_tag="O2458_002300"
     CDS             complement(7540..7743)
                     /locus_tag="O2458_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ3888148.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            8084..8344
                     /locus_tag="O2458_002301"
     CDS             8084..8344
                     /locus_tag="O2458_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888149.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            8373..8561
                     /locus_tag="O2458_002302"
     CDS             8373..8561
                     /locus_tag="O2458_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888150.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            8749..9318
                     /locus_tag="O2458_002303"
     CDS             8749..9318
                     /locus_tag="O2458_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888151.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            9406..9690
                     /locus_tag="O2458_002304"
     CDS             9406..9690
                     /locus_tag="O2458_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888152.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            9752..9970
                     /locus_tag="O2458_002305"
     CDS             9752..9970
                     /locus_tag="O2458_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888153.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT         3367 a         1634 c         1416 g         3603 t
ORIGIN      
        1 ctacgaagtt atattggcag tagttgactg aacgaaaatg cgcttgtaac aagctttttt
       61 caattctagt caggggcccc aacacagaga atttcgaaaa gaaattctac aggcaatgca
      121 agttggggat gggccccaac aaagagaaat tggattccca atttctacag acaatgcaag
      181 ttggggtggg acgacgaaat aaattttgcg aaaatatcat ttatgtccca ctccctagat
      241 tgatctatag atactacact tattaaagta atatattttt atgattctct tagctgcaat
      301 cccatgaata catgtaatca tcaaacttca tagcctcaag gtcagtagat ttcactaaga
      361 tattggcaat acctaaatca ccccatataa tcgaagaatt accatctctt gtagaatcaa
      421 tttgcagtaa gactctatca tacattttca gttcttgatc gttcgttctt ggatctattt
      481 ggtcaaaata tggataacca ccaagttgat taaatggttc cttagcagta aatgtgttac
      541 acacaggttc catcaattca tcataaccac caaacattga ttttagcgcg aaggatggat
      601 tgactttatt ccacaatgca agcacgtcct catcgtactt ataatcagta catgtaatag
      661 tttgtttcga caatctaaat tctatagaaa atgctccact gaacgggata tcctcattat
      721 cagcatctaa tgacttcaaa tgcgcttgaa tattttcgag tgataaatct gtaattggct
      781 cttttatata aacaagcctt gagtttatat tgtttccctt taaattttca gacattccat
      841 acatttgatc ttctgaacta atccaaaact gcaatatccc ttcttttaca ggaaataatt
      901 cttgttgttc tggaagatca tccaaattaa tctgtgctag caataccatt ggtacattat
      961 tttcatctaa tggtatatct ttaaaatatt ttaaaaatgg cattccacca atcttactct
     1021 ggtttaatgt ttcttcttgc atctctttta gatccatttc aatacatggt agtactggta
     1081 aattaccgtt ttctacagat tctacgaaac ggttatatgc tttttgctcc tttttagaaa
     1141 gatgactaac gtcttgaatg tttagcatat taacttcccc caatttttat ttttaaaata
     1201 gctaaattca ttatttccat cattcttgaa taataaaatg tagcaatact ccaaataata
     1261 gtacctaata atttttcttt catatcattt attttctcag actaaaaata aactcctcaa
     1321 tcttagatta aggagttaca cggtgattat tcaccaatca tttctgaata tttttcagct
     1381 gttaataaag cttcaatctg actttcatca ctaatttcta cttttaccat ccatgctttt
     1441 tcgtatggag attcatttac aaattcggga ctatcttcta gttcttcgtt gacttcaact
     1501 actttaccag agattggtgc atataattct gatacagttt taactgattc tacgctacca
     1561 aacgtatccc cttcattaat ttcatcatct gtttctggta attcaacgaa aacaatatca
     1621 cctaactcgc tttgtgcgta ttctgtgatt ccaattattg ctacattacc ttcaacttta
     1681 acccattcat gctcttttga atatttcaat tcatttggta ctgccaagag aatcccctcc
     1741 taattaatct ttacatcatt aatgatgcca taaggctaga tgctattcaa ctatatttat
     1801 aaaaattaac aattgtatag cttatttcat tttacatgta atgaatctaa atatcgagat
     1861 atttagtaaa cgcttacatt tcacttacgc taaccaagtc tctttatatt gatcttcttt
     1921 aaatcctaat gttatcttat cgcccattac tgctagagga cgctttacta acataccatc
     1981 agatgataac aactctaact tttcatcatc tgataaagtt tgtaatttat ttttcaaatc
     2041 aagctcacga tatttcgcgc cgtgtgtatt aaacaattta ttaatttcta cgcctgtatt
     2101 tgcaattatt gttttaaatt catttattgt aggtgtatgt tgaacgatat caattggttc
     2161 ataacttacg ccatattcat ctaaaaactt tgctgccttt ttacaagttg tacaattctt
     2221 atattggtaa aatttaatca tacgactgca acctccttta tgtatttaaa ataaatatat
     2281 cattttccaa agcaaaaggc tttcattttg aagctaaaat tttttattat gatataaatt
     2341 tagctataat aaaaattatt aaactatcta gaggagttgt tacaatgcaa tcaatcaaaa
     2401 gtaatgaatc atttaaatct gtaattaata gcgatacacc tgtaattgtt aaatttgagg
     2461 caggatggtg cccagactgt cgtgctatgg atttatggat tgacccaatc gtagaacaat
     2521 ataatgatta ccaatggtat actgttaatc gtgatgaatt agaagatgta gttgttgaaa
     2581 atgaagttat gggtatccct agcttgctag tatttaaaaa cggcgataaa attgcacacc
     2641 ttcattcagc aaatgctaag tcacctgaac aagttgaatc atttttagca gaaactttta
     2701 aataaatact aaaataaaaa tgtgtatata ctgcacaata aaaagctgag actgttattg
     2761 taccgactct agtaagttag attaaatctc taactcgata gatgtccatc aattcatgac
     2821 tcagctttta ttaatgtttc tataattata catattaata taatgtaatt aagtttctat
     2881 ttttacgtgg tgctttaggc atatctcctt ctaaatccgt taaatataag aatccagcaa
     2941 gaacttcatc tttctttata ccaagtgttt ttcttacttt aggatcatag atatataatg
     3001 gcgatttcca acatgtacct attcccgctt cataaagtaa caacataaga ttttgtgcat
     3061 atgcaccaaa tgcaaagtag ttttcatcat tttgacgttg tcttggatct gtttttaaaa
     3121 taagcaataa catgccacct agtttcgtaa ctgcatcata atgacattgt tgcttatcta
     3181 attcattagg aaatgcaaat ttagaaatat ccttactcat atctcctaat ctgtctttcg
     3241 gaacatgcac aactctccat ggttccctca ttccgtgatt tggagcatca gcagctttct
     3301 caattgcttg atatagcaat gcgtcatcta tgtgcatatc tcttttaaat tttttcacac
     3361 ttcttctatt agctattgct tgttgtaatt ccatctaaaa tcgctcctcc acgaacaatg
     3421 ataatacttc tcaattataa gacagcactt acacaaaagc aacctaattc attttattag
     3481 tataaagtca cttgtgcttt taaatcagta acatcaatct gtcctggagc ttgtggtact
     3541 ccgatacaac cataagtcaa cgcaccacca aaaacgcctt gagccgttct acttattagt
     3601 ccaagtttag acattgaaat accaacaact ttgcagtcca tagtatctga aaatgtagac
     3661 attgcctgca ataaatttaa cacatcattt ttattatgtg gcattactgc taatttaacg
     3721 tattctgggt tgaatttttg cattttaaaa aatataaatt gcaattcatc taatggaggc
     3781 gtactttcga aattatgatg tgatataacc acctctttat tatactgttg caaatgtgta
     3841 atgattcgtt gatgtttttc aatgtcaata tctgcttgcc attctatatc aatcatatct
     3901 atgccattga tatttgctaa gtctgatatt aaattaagat acgagtcatt tgtaaattgc
     3961 ccatagccac cttgtaactt tgtacgatac gtaactaata atttgaatga atcttgcata
     4021 accttcagct ttgtaatcat ttctgccact tgatcaactg tgacattttc aatttgatca
     4081 attcgtaatt ctaatacgtc tattgcatca atacgatgat taattttttg aattaaagtt
     4141 tcttcgatag ataattgtgg cgcgatagtc gctactactt ccacatgtgt catagtgaat
     4201 ccctcatttc agatactcat tatttttatt gtaaacaaca actttttatc cttcaatcaa
     4261 acaatgctat aaatgaaaat gattaaggta ttttcgaatg ggtaaagcat aaatgtattt
     4321 taaattagga ggttataagt atggcaatac attatgaaac taaagcgacg aatgttggcg
     4381 gacgtaaagg acatgtttat actgatgatc gagcattaga tattgatatc gttccgcctg
     4441 ctcaagcaga tggtaaagct actaaccccg aacaattatt tgcagcaggt tatgcatctt
     4501 gcttcaacgg tgctttcgac ctaattttaa agcaaaacaa agtgcgtgat gctcatccag
     4561 aagtaacact aacagtgaga ctagaagatg attcagactc agaaagtcct aaattaagtg
     4621 tttcaattga tgcgacaatt aaaaatgtta tatctcaaga agaagctgaa aaatatttac
     4681 aaatggctca tgaattttgt ccatattcaa aagcgactca aggaaatatt aatgtcgatt
     4741 taaatgtaaa tgttgtagat tagcattaac ttaaagagat tattcaacgt tattaataaa
     4801 attcacataa aattcaaatt tgccaaccaa aaatttttgg ttggttattt tttctattcg
     4861 tgattgaaat ttctggcaat atttaactga aaatgattgt accttagtca tcataaatgt
     4921 gaccggttcc aatactggct tgacttcttc gcataccgtc tacaaataaa agtccagtgt
     4981 cgaccaataa ccaattccat aatttcctga acttaaaaat aaatataatt aaattgataa
     5041 tccatttaca agaatgtgca tataaatcag tgattgcaaa attttaataa acattttaat
     5101 atgtaattaa gaaatttttt tagtacaaaa tctttcttaa taatgattaa ggaggtcagt
     5161 aatgattgaa attaaaacat taacgaataa tgattttaat gagtataaga gacttgtttc
     5221 gacagtcaat gaagaattca ctcaagattc acattatagt caaacaatga ctgacacctt
     5281 aatacatgac attttaaatc aaggttcacc gaaatgtatt gtatttggct gttatgaaaa
     5341 cgaaacactt atcgcaacag ctgccttaga acaaattcga tacgttggaa aagaacataa
     5401 atcattaatt aaatacaact ttgttactaa taacgataaa tcgattaata gcgagctcat
     5461 taatttcatt attaattatg cacggcagaa caattacgaa tctttactta catcaattgt
     5521 gtcaaacaac ataggtgcta aagttttcta tagtgcacta ggattcgaca ttcttggttt
     5581 tgagaaaaat gcaattaaaa tcggaaatac ctatttcgat gaacattggc ttttttatga
     5641 tttgattaat aagtaataca gttttatata ttctacattt ctcataaatt caaatcatat
     5701 aggttctatt tttcatacaa ctactctact attgagtagt tgttttttat ttgatatgat
     5761 aacaataagt tttttcagaa tattctatgt tttaggggtg tcaaaactta atggtaaccg
     5821 caattattca tggatttatc ttagcgattg gtctaatttt acctttaggt gcacaaaatg
     5881 tatttatttt taaccaagga gctaatcaac caaaatatag atatgtattg cctgcaataa
     5941 ttacagccgg gttgtcagac agcttactta ttattattgc agtggtagga gtatctatca
     6001 ttattatgtc tttacctgta cttcaagcaa ttatttatat agttggttta attttcttga
     6061 tgtatatggc ttggaccatt tggcatgata aaccctcaac agatggagaa actcaaatta
     6121 tgtctccaat gaaacaagta agttttgctt tatcagtttc attactcaat ccacatgcta
     6181 ttttagatac aattggagta attggtagta gtgctgcatt atatagtggc agcaataaaa
     6241 ttgcgtttac aatcgcttgt attagcgttt catggttatg gttcttttta ctcgcaattt
     6301 taggaaaaat ggttggatca attgataaaa ccggaaaatt attaacaata atcaataaaa
     6361 tatcaagcat aataattatt attgttgctc tgatgatatt acaaaaactt attcaattat
     6421 tattctaaag cgttatgtag ttgtatttta aaagcgttat acaaaagaca tacttgtgtc
     6481 tatgtataac gctttattta ttataaattt ggatcaatta actcaacaaa tttaaatgtt
     6541 ccattgtcat attcaaattt caggatatta caattaccga tattatgctt taatgcttca
     6601 tctagtttaa ggcaatatct taaaaatact cccattattg ttccatgact cacgactaat
     6661 gcattattct ttgtttgaga cataatgtca tgtaaagttt tcacaatgcg ttcttcaact
     6721 tgttgccttg cctctccttt gaaaggaaca attcgatctc cgaataagtc ctcaggcttg
     6781 tatagattat caaatagata gactgactca ccttcaaata ttccaaaatg ccattccttc
     6841 agtcccttaa aacgttgata cgattggtta ggtgcaacat tttcaagtgt gtcacttgcg
     6901 cgttcttgcg ttgatgatgc atataaatcg aagtttatcc ccttagtttc ataataacta
     6961 cgtgcctttt gagcttgagc aattccaagt tctgttagcg gcgaatctcc aaatccctga
     7021 attagtccct taaaattaaa caaagtttgt ccgtggcgca ttatatataa cgttttagcc
     7081 aacaataaca cctcttcaat aaaaagtatt gatagctcaa aatgtattct ctttttatta
     7141 aaatgagtac caaatatatt aaaccattat ttaaactttt tcggtagcga aaacctttgc
     7201 ttttgatgta catttttttc ataagtaatg atgattttag tttaaaagat aatgtgacgg
     7261 ggtaaaacgc aatgaagagt acagaatttt aaaggagtga gtgtttatgg ataatcataa
     7321 tcaatgtaat tatgtgaatc cacaaaatgt ttcacttgac tgggaatgtt ttataattag
     7381 taaaagtgaa atgttgttag atggtgtacc aaatgaactt atcaacactt ggttagataa
     7441 agacattatt acaccatttt cgataagaaa tgatgaaata aactttaaaa caaaagatat
     7501 ttgggatgca ctaatacatc ataattggta ctactcaaat taatttgcca tatataagct
     7561 aatagctcta tttgtagttt caattaattg atgatctgtg taattagaca tcacatatgc
     7621 tctaattact tggtgtcttt cttctaatcg agggaatgct ttatcttcac taacgtgttc
     7681 ggccaatata ccaaatggtg tgttatcatt ttgaaaaccc atgatgaaat tgtaaaacgt
     7741 cataagactt gcccccttca tttattaagc gctatttaaa gtgttattat taatcttatc
     7801 agaaaactag tatttgtaaa tgtgcaaaag tgattttgca catttttttg atatctgatt
     7861 ctaaaaatat taacgaatta tacaacttta agagttttta gtatgtaaat attcttttca
     7921 tattaataca atacacccct atatatgctc acttgtttga attaataaac tttagcattc
     7981 tgcaaataat attttaaaaa taactatact ttttatttac ctattgaaaa atagtgttat
     8041 catatttatt aagaagctat ttttatacta gtaggtgtca gtcatgaata acattttgtt
     8101 aaatgctatc aatatagtta ttactaccac ttttgttatc tttaatattt taatcacata
     8161 taataaagat ttagatgatt tatgttggct cctgcctggt attatcattt gtggtgtgat
     8221 actcatcgta tcctttacca ttgcaatgat aactaaaaac tggttaagtg aaatattatt
     8281 ttttataaat atcgtactcg ttctctatta catttatcct attttttata gttttatagg
     8341 ttaataatta cggtatataa ggagggtttc acatgtcttt acattttgca attctgtttt
     8401 ggctagcatt aattttctta gttgccgcta cgtttatact cgtattaatg aaaaaaacta
     8461 gcaaagaatc taaaaaagag tcctatttaa gtttcactgt cattctctat atttttggat
     8521 tcgctatatt aatatacaca tttatatttg gtgtgctata aatgaaattc attttatatc
     8581 cagtctgaaa cgtctcaatg atgtttcaga ctgtttttta ttcttaatag acatttcaca
     8641 ctctcatact ttaatattta agatatgttt tagtcttaca agcttattaa agattacaat
     8701 tctaatttaa gactatttac atatatttct tcaacaagga gcttttaagt gaactatatt
     8761 aaacgaacga taattttact tatattattc gttgtcgtat ccccaataaa ctcaccaaaa
     8821 acaattgcag ataataagta ttcagaaatt caagatgaca aattccaatt acaacctggt
     8881 gatattattg ttacaaaagg ccctgtcatg tggggatttt ttggtcattg tagtatcgcg
     8941 attgatgata aaacgatttt acaaattgag gggcctggcg acaaaccaac tacacaatcc
     9001 ttcgaatctt ttaaatataa ttatgcaagt ggcaaaaatg attggatgaa agtttatcgt
     9061 tgcagctatc ctggtgcagg taaaaaagcg gctgaatggg ttaagaaaaa ctatgagaat
     9121 acaaatcatc gttatctagt tacattaaat ttgaacagca aaaaatttac gtattgtact
     9181 aaaattatct accaagctta taaatttggt gtaagtgaaa aatcagttaa aagttatgga
     9241 ctacatatta tttcacccta tgcaattaaa gataatttta tagatccata caaactaagg
     9301 cttgttaaag cttattaaca cagtttcatc aaatagttaa attagtttgt gttttgcaat
     9361 aaattgggta tagattacaa atgatatttc aggaggccca atattttgaa tgaactaact
     9421 aaagaacaaa agtatacaat agctaaattt tacaaattat atattgaacg ttcgaataaa
     9481 ggagaaacag aaacagtcgc taactttttt ggcgatgcta aagatgcacg tgaaaactat
     9541 ttttgcgatc gtgattatca agacttctta actaactgcc aaattttaat tcaaaacaaa
     9601 tacttaactg gtgaagtttt ggacgataac atttacaata tttctatttt aaacaagaca
     9661 tttattgaat tcgaacaaaa ttttggttga acatgaatgt ctcaactata aactttctaa
     9721 ccctatttta aagcaggagt gaaaaacata catgagtgaa tggcatatta ataatcaatc
     9781 tacaaaacct tttttaatac aacaagcgga aaagcaattg tctattgtta aaccgttgcc
     9841 taatggtagc ttccaaatac tcacagaaat aaatttggag aatggcaata ttagtaacat
     9901 cgatcattct ttacttgttt cagtagaccc caaagcatta gaaataagta tatttgacgc
     9961 tgtaaattaa acgcaatatt tttatttaat tattttaatg tttcgccttt taaataaaaa
//
LOCUS       DALAAR010000086         9889 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000086 DALAAR010000000
VERSION     DALAAR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.088"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            48..482
                     /locus_tag="O2458_002306"
     CDS             48..482
                     /locus_tag="O2458_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ3888154.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(739..924)
                     /locus_tag="O2458_002307"
     CDS             complement(739..924)
                     /locus_tag="O2458_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCZ3888155.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            1219..2160
                     /locus_tag="O2458_002308"
     CDS             1219..2160
                     /locus_tag="O2458_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCZ3888156.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2172..3416
                     /gene="fabF"
                     /locus_tag="O2458_002309"
     CDS             2172..3416
                     /gene="fabF"
                     /locus_tag="O2458_002309"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCZ3888157.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(3471..3842)
                     /locus_tag="O2458_002310"
     CDS             complement(3471..3842)
                     /locus_tag="O2458_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCZ3888158.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            4085..5011
                     /locus_tag="O2458_002311"
     CDS             4085..5011
                     /locus_tag="O2458_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888159.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            5011..6081
                     /locus_tag="O2458_002312"
     CDS             5011..6081
                     /locus_tag="O2458_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888160.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            6098..7180
                     /locus_tag="O2458_002313"
     CDS             6098..7180
                     /locus_tag="O2458_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888161.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            7170..8111
                     /locus_tag="O2458_002314"
     CDS             7170..8111
                     /locus_tag="O2458_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3888162.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            8130..9785
                     /locus_tag="O2458_002315"
     CDS             8130..9785
                     /locus_tag="O2458_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3888163.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
BASE COUNT         3554 a         1430 c         1874 g         3031 t
ORIGIN      
        1 tatattgatt ttgtaagata ctttaactca caaaggagag agattatatg aaattaaaat
       61 catttataac tgtaactttg gcactgggca tgatcgcaac gactggcgct actgtggcag
      121 gtaatgaggt atctgcagca gaaaaggaca aactaccggc aactcaaaaa gctaaagaaa
      181 tgcaaaatgt tccatataca attgcagtag atggcattat ggctttcaat caatcttact
      241 taaatttacc aaaagatagc caattatcat atttagattt aggaaataaa gttaaagctt
      301 tgttatatga tgaacgcggt gtaacacctg agaagattcg aaatgcaaaa tctgccgttt
      361 acacgattac ttggaaagat ggtagtaaaa aagaagtgga tcttaagaaa gatagctaca
      421 cagcaaactt gtttgattca aattcaatta aacaaattga tattaatgta aaaactaaat
      481 aaacacttaa aattatcaaa ttcaacatga agcatttaat tttacagtga tgattataaa
      541 ataattgcct tgatacaaag attactcgta aatgacatct ttgtattaag gctttttcta
      601 aatttaaaag tgatgggtta gaggtcattg agctttaaaa tattcaaaat acaaaacatt
      661 aatggccaaa aataaaagcc gcctttatct gggcagcttc aataataaga aagacatatt
      721 tcattttata ctaaatagtt attgtgatga atctttcggc ggtttaatta ctgcagcaaa
      781 aattgctgtg aaaatcgtga acaatactgc catgataatt ggattcacta catttaagct
      841 gtctccacct actaggctat taagtacaaa gttaaccatt tgcattaata ataatgccca
      901 aaagaatgtt acgaggtgtt tcatgtcatt ctacctccac tttaattata tatattttat
      961 tttaagtgaa agttagaaat ttgtatagta acatctcata tattttgacc atattataca
     1021 gtttaaataa atgattttat ctgaatggct attctaaatt aagcgcatta aaaccaattt
     1081 catactgaaa tttgacgata ataaagcatt aaaattttat taactagtca atattcctac
     1141 ctctgacttg agtttaaaaa gtaatctatg ttaaattaat acctggtatt aaaaatttta
     1201 ttaagaaggt gttcaactat gaacgtgggt attaaaggtt ttggtgcata tgcgccagaa
     1261 aagattattg acaatgccta ttttgagcaa tttttagata catctgatga atggatttct
     1321 aagatgactg gaattaaaga aagacattgg gcagatgatg atcaagatac ttcagattta
     1381 gcatatgaag caagtttaaa agcaatcgct gacgctggta ttcagcccga agatatagat
     1441 atgataattg ttgccacagc aactggagat atgccatttc caactgtcgc aaatatgttg
     1501 caagaacgtt tagggacggg caaagttgcc tctatggatc aacttgcagc atgttctgga
     1561 tttatgtatt caatgattac agctaaacaa tatgttcaat ctggagatta tcataacatt
     1621 ttagttgtcg gtgcagataa attatctaaa ataacagatt taactgaccg ttctactgca
     1681 gttctatttg gagatggtgc aggtgcggtt atcatcggtg aagtttcaga tggcagaggt
     1741 attataagtt atgaaatggg ttctgatggc acaggtggta aacatttata tttagataaa
     1801 gatactggta aactgaaaat gaatggtcga gaagtattta aatttgctgt tagaattatg
     1861 ggtgatgcat caacacgtgt agttgaaaaa gcgaatttaa catcagatga tatagattta
     1921 tttattcctc atcaagctaa tattagaatt atggaatcag ctagagaacg cttaggtatt
     1981 tcaaaagaca aaatgagtgt ttctgtaaat aaatatggaa atacttcagc tgcgtcaata
     2041 cctttaagta tcgatcaaga attaaaaaat ggtaaaatca aagatgatga tacaattgtt
     2101 cttgtcggat tcggtggcgg cctaacttgg ggcgcaatga caataaaatg gggaaaatag
     2161 gaggataacg aatgagtcaa aataaaagag tagttattac aggtatggga gccctttctc
     2221 caatcggtaa tgatgtcaaa acaacatggg agaatgctct aaaaggcgta aatggtatcg
     2281 ataaaattac acgtatcgat actgaacctt atagcgttca cttagcagga gaacttaaaa
     2341 actttaatat tgaagatcat atcgacaaaa aagaagcgcg tcgtatggat agatttactc
     2401 aatatgcaat tgtagcagct agagaggctg ttaaagatgc gcaattagat atcaatgaaa
     2461 atactgcaga tcgaatcggt gtatggattg gttctggtat cggtggtatg gaaacatttg
     2521 aaattgcaca taaacaatta atggataaag gcccaagacg tgtgagtcca tttttcgtac
     2581 caatgttaat tcctgatatg gcaactgggc aagtatcaat tgacttaggt gcaaaaggac
     2641 caaatggtgc aacagttaca gcatgtgcaa caggtacaaa ttcaatcgga gaagcattta
     2701 aaattgtgca acgcggtgat gcagatgcaa tgattactgg tggtacagaa gcaccaatta
     2761 ctcatatggc aattgctggt ttcagtgcaa gtcgagcgct ttctacaaat gatgacattg
     2821 aaacagcatg tcgtccattc caagaaggta gagatggttt tgttatgggt gaaggtgctg
     2881 gtattttagt aattgaatct ttagaatcag cacaagctcg aggtgccaat atttatgctg
     2941 agatagttgg ctatggtact acaggtgatg cttatcatat tacagcgcca gctccagaag
     3001 gtgaaggtgg ttctagagca atgcaagcag ctatggatga tgctggtatt gaacctaaag
     3061 atgtacaata cttaaatgcc catggtacaa gtactcctgt tggtgactta aatgaagtta
     3121 aagctattaa aaatacattt ggtgaagcag ctaaacactt aaaagttaac tcaacaaaat
     3181 caatgactgg tcacttactt ggtgcaacag gtggaattga agcaatcttc tcagcgcttt
     3241 caattaaaga ctctaaagtc gcaccgacaa ttcatgcggt aacaccagat ccagaatgtg
     3301 atttggatat tgttccaaat gaagcgcaag accttgatat tacttatgca atgagtaata
     3361 gcttaggatt cggtggacat aacgcagtat tagtattcaa gaaatttgaa gcataactat
     3421 aaaaatcttc agtaacgttg ttttagttac tgaagatttt ttcagtttct ttatactaag
     3481 atgagcgaca gcacaatcgt cataataaaa tataaaatat ttattaataa taaggggatt
     3541 atccatgtag aaacaaagta atgctctttt tttacctctt gtgggttgaa aaatggatca
     3601 tcagagatag acttcttctt tttcgaagat gacatttgat actttaatct tctaaaacca
     3661 taacttgtcg catcaaaaat gccttcttgt acaagtaaaa tcaaaaatat gctaataaaa
     3721 ataattaatg aaacataaaa caatatattt aaatatgtaa tgatagtatg gctattaaaa
     3781 agccatataa taaacgttaa tattggcgtt attagtgcca ttccaagcca ttttttcaac
     3841 atttgatcac tcccacttat agaaaactct tacgcatagt ttacattaaa atcagacatt
     3901 gaggaatgat tttttaattt cttcagcttt attgaaattc taaaatcaat cattcttcat
     3961 tagtttaaag caaaaaaata ttgatatata gtaaatattg tatatataat attagttaag
     4021 atttcagaaa attttgaagg gaatggaaat ttagaaatcg gaatttgtta gaggagggga
     4081 ttagatgggg aaatatattt tcaaacgatt tatttatatg cttatttctt tatttattat
     4141 tattacaatt acatttttct taatgaaatt aatgccaggt tcgccattta acgatgctaa
     4201 attaaatgct gaacaaaaag aaattttaaa tgaaaaatat ggattaaatg atcctgtagc
     4261 tacgcagtat ttacattatt taaaaaatgt tgttacaggc gattttggta attcattcca
     4321 gtatcataat caacctgtgt gggatttgat taaaccgaga ctactacctt cttttgaaat
     4381 gggtcttaca gcaatgttca tcggtgtgat actgggactt attttaggtg ttgcagcagc
     4441 tactaaacaa aattcttggg ttgactatac aactacagtt atttcagtta ttgcagtatc
     4501 tgtaccatct tttgtacttg ctgtactttt acaatatgta tttgcagtta aattaagatg
     4561 gttcccagta gctggatggg aaggtttttc gaccgcggta ttaccgtcac ttgcattatc
     4621 tgcagctgtt ttagcaactg tcgccagata cataagagca gagatgatag aggtattaag
     4681 ttcagactat attttattag cgagagctaa aggtaattcg acaatgcgtg tactttttgg
     4741 acatgcactt agaaatgctt taattccaat tattacaatt atcgttccca tgttagcaag
     4801 tattttaaca ggcactttaa caattgaaaa tatttttgga gttcctggat taggggatca
     4861 attcgtacgt tcaattacaa caaatgattt ctcagtaatc atggcaatca cactattatt
     4921 tagcacactg tttatcgttt ctatttttat tgtagatatt ttgtacggtg tgatagatcc
     4981 acgaattcgt gttcaaggag gtaaaaaata atggctgaaa ataaaaacaa tttgtcgatt
     5041 aacgacgatc attctaatgc agctatgacg catacctctg acgctatcgc atcatctgat
     5101 tttattatta gagaattaga tttgaatcag gaacctgaaa tgcaacgaga aagcaaaaac
     5161 ttttggcaag atgcttgggc tcagttaaaa cgaaataagt tagctgttgt cggtatgata
     5221 ggtttaatta tcattgtaat atttgctttt atcggtccag ttataaataa acatgattat
     5281 gctgaacaaa atgtagaaca tagaaatctt ccggcaaaaa tacctgtatt agacaaagtt
     5341 ccatttttac cttttgatgg taaagatgca gatggcaagg atgcttataa agcagcaaat
     5401 gctaaagaaa attattggtt tggtactgat cagttgggtc gagatttatg gacaagaaca
     5461 tggaaaggtg ctcaaatttc attgtttatc ggtgttgttg cagcgatgtt agatattttt
     5521 attggtgttg tatatggtgc gatttctgga ttcttcggtg gacgtgtcga tacgattatg
     5581 caacgtatac ttgaagtcat agcatctatt ccgaatttaa ttgtcgtaat tttatttgta
     5641 ttaatttttg aaccatccat ttggacaatt atattggcta tgtctatcac aggctggtta
     5701 ggcatgagca gagttgtacg tggagaattt ttaaaattaa aaaatcaaga gtttgtcatg
     5761 gcttcgaaaa cattgggggc ttcaaaattc aaattgatat ttaagcatat tttacctaat
     5821 acattaggtg ctatcgtggt tacatcaatg tttacagtac ctagtgctat tttcttcgaa
     5881 gcatttttaa gtttcattgg tataggtgta cccgcacctc aaacatcgtt agggtcatta
     5941 gtaaatgatg ggcgcgcaat gttattaatt tatccacatg aattatttat accagcaatg
     6001 attttaagtt tattaattct attcttttac ttatttagtg atggattacg tgatgcattt
     6061 gatccgaaaa tgcgtaaata aaaagggggc atagcatatg actgaaagaa tattagaagt
     6121 aaatgatttg catgtttcct ttgatattac agcaggggaa gtgcaggcag tgagaggcgt
     6181 agatttttat ttgaacaaag gggaaacatt ggcaattgtt ggtgaatcag gttcaggtaa
     6241 atctgtaaca acaaaagcaa ttacaaaatt attccaaggg gacacaggaa gaattaaaaa
     6301 gggagaaatt ttatttttag gggaagattt agcaaaaaaa cctgaaaatg agttgattaa
     6361 attacgtggc aaagatattt caatgatctt tcaagatcca atgacatctt taaacccaac
     6421 gatgcaaatt ggtaaacaag tcatggaacc attaattaag cacaaaaatt atagtaaagc
     6481 acaagctaaa aagcgcgcat tggaaatact aaatcttgta ggtttaccaa atgcagaaaa
     6541 aagatttaaa gcatatcctc atcaattttc aggtggacaa aggcaaagaa ttgttattgc
     6601 aaccgcatta gcttgtgaac ctaaagtgct cattgctgat gaaccaacga ctgcattaga
     6661 cgtaacgatg caggcacaaa ttttagattt aatgaaagaa ctacaacaaa aaatcgatac
     6721 agcaattatt tttataacgc atgatttagg ggttgttgcg aatattgctg atagagtggc
     6781 agttatgtat ggtggtcaaa tggttgaaac aggagatgtt aacgaaatat tttatgatcc
     6841 aaagcatcca tatacatggg gattattatc gtcaatgcct gatttatcaa caacaaatga
     6901 cacaccatta ctagcgattc ctggagcgcc acctgattta ttacacccac ctaaaggtga
     6961 tgcatttgcg agacgtagtc aatatgcatt agatattgat tttaaagtag aaccaccgtg
     7021 gtttaaagtt tcaccgacac attttgtgaa atcttggtta ttagacgcac gtgcaccaaa
     7081 agttgaacta cccgagctgg taaaacaacg tatgaaaccg atgcctaata attatgaaaa
     7141 accactcaag gtagaaaggg tgtcgttcaa tgaaaaatga tgaagtgcta ttatctatta
     7201 aaaatttaaa gcaatatttt aacgcaggaa agaaaaacga agtgagagcg attgaaaata
     7261 tttcgtttga tatatacaaa ggggaaacat taggtttagt aggagaatcg gggtgtggta
     7321 aatctacaac tggtaaatca attattaaac ttaatgatat tacaagtgga gaaattttgt
     7381 atgagggtat tgatatacaa aagattcgta aacgtaaaga tttgcttaaa tttaataaaa
     7441 agatacagat gatttttcaa gacccatatg cgtctttaaa tcctaggtta aaagtaatgg
     7501 atatagtagc tgaaggtatt gatatccatc atttagcaac tgataagcgt gaccgaaaaa
     7561 aacgtgtcta tgatttactt gaaactgttg gattaagtaa agaacatgcc aatcgctatc
     7621 ctcatgaatt ttcaggtgga caacgccaac gtattggaat tgcccgtgca ttagccgttg
     7681 aaccagaatt cattatcgcg gacgaaccaa tatcggcatt ggatgtttca atccaagctc
     7741 aagtagttaa tttattatta aaattacaac gtgaaagagg gattacgttc ctatttatag
     7801 ctcatgatct atcaatggtg aagtatattt cagatcgtat tgcagtcatg cattttggga
     7861 aaatagttga aattggaccg gcagaagaaa tttatcaaaa tccattacac gattatacta
     7921 agtctttatt atcagccatt ccacaacctg atcctgaatc agaacgcagt cgcaaacgat
     7981 ttagttatat tgatgatgaa gcaaataatc atttaagaca attacatgaa attagaccga
     8041 atcactttgt ctttagtact gaagaagaag cggcacaact acgagaaaat aaattggtga
     8101 cacaaaatta aggggaaggg ggaaatgcaa tgacgagaaa atttagaaca cttattttaa
     8161 ttttgattgc tacaattgca ttaagtggtt gtgctaatga cgatggtatt tattcagata
     8221 aaggtcaagt attcagaaaa attttgtcat cagacttaac atcccttgat acatcattaa
     8281 taacggatga aatatcttct gaagtgactg cgcaaacatt cgaaggttta tacacattag
     8341 gaaaaggtga caaaccggtg ttaggtgttg cgaaagcttt tcctgaaaag agtaaagatg
     8401 gtaaaacttt aaaggttaaa ttaagaagcg atgctaaatg gagcaatggt gacaaagtga
     8461 ctgcacaaga ctttgtttat gcttggagaa aaacagttga ccctaaaaca ggttctgaat
     8521 ttgcatacat tatgggggac attaaaaatg cgagtgatat tagtactggt aagaaacctg
     8581 tagagcaatt aggtatcaaa gcattaaatg atgaaacatt acaaattgaa ttagaaaagc
     8641 cggttccata tattaatcaa ttattagcac ttaatacctt tgcacctcaa aatgaaaaag
     8701 ttgccaaaaa atatggtaaa aattacggta cggcagctga tagagcggta tacaatggtc
     8761 catttaaagt tgatgattgg aaacaagaag ataaaacctt actatctaaa aatcagtatt
     8821 attgggataa aaagaatgta aaattagata aagtgaatta taaagttatt aaagacttac
     8881 aagccggtgc atcattgtat gatactgaat cagtagatga cgcagttatt actgcagatc
     8941 aagtaaataa atataaagac aacaaaggat taaactttgt gttaacgact gggacatttt
     9001 ttgtaaaaat gaatgaaaaa caatatcctg attttaaaaa caaaaattta agattggcta
     9061 tcgcacaagc aatagataaa aaaggatacg ttgattcagt gaaaaacaat ggctcaattc
     9121 cttccgatac actaacagcc aaaggaattg cgaaagcgcc taatggcaaa gattatgcga
     9181 gtaccatgaa ttcgccttta aaatataatc ctaaagaagc aagagcgcac tgggacaaag
     9241 ctaaaaaaga gttaggtaaa aatgaagtga cattttcaat gaacacagaa gatacaccag
     9301 atgcaaaaat atctgctgaa tatatcaaat cgcaagttga gaaaaattta ccaggagtta
     9361 ctttgaaaat taagcaatta ccgtttaaac aaagagtatc actagaactg agtaacaatt
     9421 ttgaagcatc acttagtggt tggtctgcag attaccctga tcctatggct tatttagaaa
     9481 caatgaccac aggtagcgca caaaataata cagactgggg taataaagaa tatgatcaat
     9541 tacttaaagt agcaagaacc aaattggcac ttcaaccgaa cgaacgatat gaaaacttga
     9601 aaaaagcaga agaaatgttc ctaggagatg caccggtagc accaatttat caaaaaggtg
     9661 ttgcacattt aacaaatcct caagtaaaag gattaattta ccataaattt ggtccaaata
     9721 actcacttaa acatgtatat attgataaat cgatagataa agaaacaggt aagaagaaaa
     9781 aataatatgc tttgtaaatt aggctggaga catatctcca gtctttttgt gttggataaa
     9841 aactttggga ataaaaattt aaaataagtc gttttttaaa ttactgaaa
//
LOCUS       DALAAR010000087         9848 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000087 DALAAR010000000
VERSION     DALAAR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.089"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(134..1042)
                     /locus_tag="O2458_002316"
                     /pseudo
     CDS             complement(134..1042)
                     /locus_tag="O2458_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1267..1479)
                     /locus_tag="O2458_002317"
                     /pseudo
     CDS             complement(1267..1479)
                     /locus_tag="O2458_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1554..3089)
                     /locus_tag="O2458_002318"
     CDS             complement(1554..3089)
                     /locus_tag="O2458_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ3888164.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3140..4684)
                     /locus_tag="O2458_002319"
     CDS             complement(3140..4684)
                     /locus_tag="O2458_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ3888165.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4913..5869
                     /locus_tag="O2458_002320"
     CDS             4913..5869
                     /locus_tag="O2458_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ3888166.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6083..6856
                     /gene="phnC"
                     /locus_tag="O2458_002321"
     CDS             6083..6856
                     /gene="phnC"
                     /locus_tag="O2458_002321"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888167.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            6858..7658
                     /gene="phnE"
                     /locus_tag="O2458_002322"
     CDS             6858..7658
                     /gene="phnE"
                     /locus_tag="O2458_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ3888168.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7655..8470
                     /gene="phnE"
                     /locus_tag="O2458_002323"
     CDS             7655..8470
                     /gene="phnE"
                     /locus_tag="O2458_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ3888169.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8601..9779)
                     /gene="deoB"
                     /locus_tag="O2458_002324"
     CDS             complement(8601..9779)
                     /gene="deoB"
                     /locus_tag="O2458_002324"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCZ3888170.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         3199 a         1782 c         1543 g         3324 t
ORIGIN      
        1 ttattattca aaaggaaaac agaaatatct ttcaatatta ttataaacat ttcaactact
       61 tttaaaaacc aacaaaaaaa tacttatttt aagtagatga gcataagtga acatagttct
      121 ttagttataa taattaattc aaccaaaagt cgatttgttt ttgcaattgg ttttcatttc
      181 ctcttaaaga tattttcatt aaatctgtca aatcaataga cgctatattt ttcaacttat
      241 ctctatattt atttttagta cgtctttcta aatttcccca ttcctcttct tcgtgagtta
      301 ataaatgaag cattgctcgt tcttgtatat tttcaatcat ttttaaattc ggttttaaaa
      361 tatgcaaatc atcaaaacaa tctttccaac aatcaaccat atctcgtttt aattcaattt
      421 ccacacgcca tagaaatgtt gaatcaattt caacatctgc attatcttta cgttcttgtt
      481 tttattataa atccgaataa acctatcact attacgcaca ccaaaatatt ttgtttctgg
      541 ttttacatta cgtccataaa atatagtttt ctttaccgac ttatctgaca atgcataata
      601 gtcatttaaa tcaaattcaa aatcaaaagc caaatctaat ctcgtaaaac taacatcgtc
      661 caaataactg atgatatttt gttttaacca aagcacttca tcatgcgaaa gcttattagg
      721 attaaattca acgcgcatac tacgtctatt ccaaagagtt gcttttattt tgtcatattc
      781 aatataaact ttttctttaa gagctttagc tttaaagttt gtttgtaaaa tatcccaaag
      841 ccgaatttca ggattagtac tcataaaatg tgaaagtctc tctgcgttag acatgctaag
      901 attcccaaca atcgttatag cgtcaaaaga caattttgga atagctagtg acatcctatg
      961 tcgatttaac cggctattac cggatattag agtatccagt tttacaaatg gatgaaacga
     1021 aattcaaaac actaaaaaat atgttccact aacagcaaaa aaataccatt atgttcctac
     1081 taaaaaacta aaaatactgg agaacaaatg tcaggatata acttaggata ctatgtaata
     1141 aaaatttaca ataaaaaaac aggaaaacaa atttcaagta aaagaatacc catacaaaga
     1201 ggataaaata aaaaacctcg aactgaaatg atgatctttt cagctcgagg tttaaatatt
     1261 ggtgccttat ttatatagat tcgttatatt atattctcta ttttcattaa cataatcctt
     1321 aaagagtttt aaattaatac ctgctagatg attcaaaaat gtttcatcaa cttttaaata
     1381 attcaataat ttttgtggtg tcagtaaatt tctatcaaaa tacaacttta ataaactatt
     1441 cattttgaca ggacgtgaca tttcaatcac gtcgtctaaa gataatactt tctcgcttta
     1501 aacaaaaaca aaaacttacc cgattaaaat caagtaagtt ttacatatat ccgtcacttt
     1561 tcaactttaa aatcaacaac ttgcgggatg cgcatcaaat tattatttga taaaaaatca
     1621 ataagtaatt gtgcgccttc aacttgaata tcttttacaa ctggcgcgtc gatatacata
     1681 tcatactgac caccgcctac tgcacgataa ttatttacac aaattgtata tgtctgcttt
     1741 aaatcaactg cgtgaccttg aatcatcata ttgctcacac gttgtccctt tggtcttcca
     1801 acatgaatgg tataacttac gccaccatat atatcataat taaagtgttg tggtttgggt
     1861 tcaaggaagt ctgcgctcac actaacttca tcatttttca cgtcaaaata ttctgctgat
     1921 cgttcaatgg cttctttaag tttggcacca cttacagcta aaactttaaa tgtatttgga
     1981 aatgggtaat tgttaataac atctcgcatc gtcacgactt gcttgaaacc actagcagaa
     2041 tcaaacaaag ctgtacaggc aacatctgcg tcactttttt ctaataaagc gtaattcata
     2101 aaatttgtaa aaggatgcgg tgccacacgt gcctcaaatg catgattaat cgtcatatca
     2161 tatggcaatg tagtaatttc gtaatctaac cagtcctcta actgctttcg taaatgttgg
     2221 tcatcttcat caatagtaaa tgtggaatca tctataacag gaagtaattc acatgattca
     2281 acggatagat tttcatattc atcagtactc aagactactc tgcctacagt tgtacctctc
     2341 gtaccaggtt gaatcacagc cgtttgctta aacctttcag caatttgtcg atgttggtga
     2401 cccgtaataa agatatctat atctttagaa aacgcttcta acatggcata tccttcattt
     2461 tcacccgtta atacttcggt cggcgtacca ctttctaaat ccttttcaaa tccaccatgg
     2521 taacaaacca caatgatatc tgcatgtcgc ttcatttcag gtaagtattg ttgaagtatt
     2581 tcaaaagcac tatgaaacgt aagtgactga atatgctctg gttgttccca atggggaata
     2641 aattgtgtcg ttaaacctat cacaccaaca gtttgatctc caacctgaaa atacttcaca
     2701 ccgttatcag tcaatgtact atcattttca tatatattag cgcacaaaac tggataattg
     2761 agtctgcgta aagtgtcttt taagtatggt aatccataat taaattcatg attaccaagc
     2821 gtaccaaagt cgaatgccat tcgattataa aaatcaacta aaggctggct actgccgcta
     2881 tgcgcgatta agtaattaca aaatggtgac ccttgcaaaa aatcaccatt atctatttta
     2941 aaactttggt catactgcct tctgtcttgt tctataacat gattcgctag taacaatccc
     3001 ataggttgat attgatttct actcgtaaaa tctgttggga aaatataacc atgtacgtca
     3061 ctcacgacat aaaatgctat gtttgacatc ctcactcact ccttcaatca caaacatctt
     3121 tcttatttct attatatatt tatttgaagt ctgttgtaat caaggttttg tcaccgagtt
     3181 ttaaacgaat ctttgaacct tccatacttt caagtacttt agcattgacc ttaattgtga
     3241 catttccgtt ttcatctgct ttaactgttg gcaaagtact gtaacctggt gggttataat
     3301 cgttatcttt acttgaaaat tgtccgattt gacgtccgcc ttctttgtaa ataccaactc
     3361 taagattttc aagtgtttga tttgcttcaa atccttttaa atgtattgtc atttcaaatg
     3421 gctcatttac agaaacattt tgcgggatat caaatgttac tttttcgttc tgatttggtg
     3481 gtgtatgatc atctggtgtg tttggctgag gatctgcgcc tttttcgctg ccataactac
     3541 ctgctttaaa tgttgttgga tcataccatt tataaccact cggcggttgt gaccatggct
     3601 ctttttcagg ctcagttgaa cgctctggtc gttcaaaatc aagcaactta gtctttgtat
     3661 ctaatgttag gctactcgcc ttaagtgatt tcccatcatt atctttagac atccaagccg
     3721 ttatattatt taatagctta ccgttgtctt gttctttaaa accatcatat gttttcttct
     3781 tttctccatt atcttctctt acatatttgg gcgaactatc ttccacaagt gatgaatcac
     3841 cgataaatgc tgctttacct tttccaactt tagaaattgc tacatagggg ccttctgctt
     3901 taccgccccc attataaata ccttgatcta cagcatgtga ccatttactt ttcgctggca
     3961 attgttctgg tgtatacaca ataccttttg ctttctctgg attagtaatt gctaatgtcg
     4021 atccggcatg catagagaca gatttcacac cttcagtaat accgaaactt tcttttgaag
     4081 aaacaatatt gctcgtattt aaatcaccta gtgcattata tcgaaaacgt acgccaaagt
     4141 ttgtagataa ccaatctgaa cttttcacac cttgcattgc agtagaactt ttttcttctg
     4201 cattcatacc tttcgacata tcttcatatg ctccacgtcg ataaccattc attgcctccg
     4261 atgaatcaat acgatttaaa tttcggtcag cattgtaatg atctgaaata aagacaacat
     4321 tgccaccttg tttcacatat ttaacaattg ctgcctgttc tgattctttg aaaggaatgt
     4381 tagcctcagg aattacaaat attttggaac ttttcaaact tgcttctgtt atgttcgaat
     4441 gaccatcaat agctttaacg tcataacctt gtttttgtat tgaatccgca taatctgaaa
     4501 atgcaccatc actaacccaa tctgcagcac cagctgtttg accatgagaa cgatcgaata
     4561 ataccgttcg ctgttgcttt gtaggttgcg attcatgcgt tatagctaaa gattgcggta
     4621 aagcacttaa tgataccgtt gcaacaattg cagagacagt taatgactta tatatttttt
     4681 tcattttgtg aggctccttt taaaataaat ttgttcttga attataggat aaaaattcgt
     4741 tgcatatgag caatttaacg aaaaatttac aaaatcttat caaactctta aagaaagtta
     4801 ttaaaattca tttttataaa atacttttta acatttaaat gtggtacgct ataagtgtaa
     4861 tttcattgca tacatattac acgattaaga atgtgaaggg gacagttatc aaatgaaaaa
     4921 ttttaagtgt ttatttgtat taatgttagc agtcattgtt tttgcagcag catgtggaaa
     4981 ctcaagttct ttagataatc aaaagaacgc tagtaatgat tcggattcta aatcaggagg
     5041 atacaaacct aaagaattaa ccgttcaatt tgtaccttcg caaaatgctg gaacattaga
     5101 agctaaagca aaaccattag aaaaattact atctaaagaa ttagggattc cagttaaagt
     5161 gtctgtatca actaactaca atacaattgt tgaagctatg aagtctaaaa aagttgatgt
     5221 tggtttctta ccaccaacgg catacacatt agcacatgat caaaaagcag ctgatttatt
     5281 attacaagca caacgtttcg gtgtaaaaga agatggttca gcaagtaaag aacttgtaga
     5341 tagttataaa tcagaaattc ttgttaaaaa agactcaaaa attaaaagct tgaaagattt
     5401 aaaaggtaag aaaattgcct tacaagatgt aacatcaact gctggatata cattcccact
     5461 tgcgatgtta aaaaacgaag caggtattaa tgcaactaaa gatatgaaaa ttgtgaatgt
     5521 taaaggtcat gaccaagcag ttatctcatt attaaatgga gatgtagatg ctgcggctgt
     5581 atttaacgat gcacgtaata ctgtgaaaaa agaccaacca aatgtattta aagacacacg
     5641 aattttaaaa ttaacacaag ctattccgaa tgacacaatt tctgtaagac cagatatgga
     5701 taaagatttt caagaaaaat tgaaaaaagc ttttatagac attgctaaat caaaagaagg
     5761 tcacaaaatt attagcgaag tttattcaca tgaaggatac acagaaacga aagattcaaa
     5821 tttcgacatt gtaagagagt acgaaaaatt agttaaagat atgaaataat cattatttaa
     5881 caaatgaatc attagcgaat ttggtattaa aagctttcgt tcaatagata tattctagat
     5941 taatattgaa aagctaggcg ctaaactgaa acagatatag aaaggtgtcg ctgtacattt
     6001 gaaaccattt gtacacagaa acccaatgtc tatgatattt cagtttacct tggcttttct
     6061 ttattaaaga aaggtgtcaa acatgagtca aatcgaattt aaaaacgtca gtaaagtcta
     6121 tcctaacggt catgtaggct tgaaaaatat taacttaaat attgaaaaag gtgaatttgc
     6181 agttattgtc ggactatctg gtgctgggaa atccacgtta ttaagatctg taaatcgttt
     6241 gcatgatatc acgtcaggtg aaattttcat ccaaggtaaa tcaatcacta aagcccatgg
     6301 taaagcatta ttagaaatgc gccgaaatat aggtatgatt ttccaacatt ttaatttagt
     6361 taaacggtca agtgtattac gaaatgtact aagtggacgt gtaggttatc accctacttg
     6421 gaaaatggta ttaggtttat tcccaaaaga agacaaaatt aaggcaatgg atgcactaga
     6481 acgcgtcaat atcttagata aatataatca acgctctgat gaattatcag gtggccaaca
     6541 acaacgtata tctattgcac gtgcgctatg ccaagaatct gaaattattc ttgcagatga
     6601 accagttgct tcattagacc cattaactac gaaacaggtt atggatgatt taagaaaaat
     6661 caaccaagaa ttaggcatca caattttaat taatttacat tttgttgact tggcaaaaga
     6721 atatggcaca cgcatcattg gtttacgtga tggtgaagtt gtctatgatg gtcctgcatc
     6781 tgaagcaaca gatgacgtat ttagtgaaat atatggacgt acaattaaag aagatgaaaa
     6841 gctaggagtg aactaacatg cctttagaaa tacctacaaa gtatgactcc cttttaaaga
     6901 aaaaggtttc tttaaaaacg agttttacct tcatgttaat cattgtgctt atcatttgga
     6961 gcttcattta tacgggattt aattttggag atttaatgat aggaatacct caaataggtg
     7021 atctattcaa acaaatgatt ccacctgatt tcgagtattt acaacaaatt acaacgccaa
     7081 tgttagatac cattcgaatg gctatcgtaa gtacagtatt aggtagcatc gtttcaatac
     7141 caattgcgtt attatgtgct agcaatatcg ttcatcaaaa gtggatttca ataccctcgc
     7201 gctttatttt aaatatagtt cgtactattc cagatttgtt attagcagca atctttgtgg
     7261 ctgtatttgg aatcggtcaa attccaggga tattagcact gtttatttta actatctgta
     7321 ttattggaaa attattatat gaatcattgg aaacgataga tccaggtcca atggaagcaa
     7381 tgacggctgt tggcgctaat aaaataaaat ggattgtttt cggtgttgta ccacaagcca
     7441 tatcgtcatt tatgtcatac gtattatatg catttgaagt aaatatacgt gcttcagctg
     7501 tgcttggatt agtcggcgct ggcggtattg gattgtttta tgatcaaaca cttggtttat
     7561 ttcaatatcc aaaaacagca acgattattt tatttacttt agttatcgtc gtcgtcattg
     7621 attacatcag tacgaaagtg agggcacatc tcgcatgaca caggaaatag caaaatataa
     7681 tgttcacaca aaagcacaca aacgaaaatt gattaaaaga tggcttattg caattgtcgt
     7741 cttagctatt atcatctggg catttgcagg tgtaccaagt ttagaactta aaagtaaatc
     7801 attagaaatc ttaaaatcca tattcagcgg attattccat cctgatatca gctatatcta
     7861 tataccagat ggcgaagact tattacgtgg tttacttgaa acctttgcga tagccgttgt
     7921 aggtactttc atcgccgcaa ttatctgtat tccattagca tttctaggtg caaataatat
     7981 ggtaaagcta cgcccagttt caggtgttag caaatttatt ttaagtgtta tacgtgtctt
     8041 cccagaaatt gtaatggcac ttatatttat caaagctgtt ggcccaggtt cattttcagg
     8101 tgtattagct ttaggtatcc attccgtagg tatgcttggg aaacttttag ctgaagatat
     8161 tgaaggtcta gatttcagtg ctgtagaatc attaaaggcc agtggtgcga ataagattaa
     8221 aacactcgta tttgcagtca taccacaaat tatgcctgcc tttctatcac tcatacttta
     8281 tcgctttgaa ctaaacttac gttcagcttc tatactgggg ctaattgggg ctggtggtat
     8341 cgggacacca ctcatatttg ccattcaaac acgttcttgg gaccgtgtag gtattatatt
     8401 aatcggttta gtactaatgg tcgcaattgt cgatttaatt tccggttcaa tccgaaaacg
     8461 tattgtttaa cattaaatca ggatactcct aaataagaag tcctaccgtc ttacgtttct
     8521 ctattataat aaaaacagca gtgaagaaaa ctattgttat agttaacttc actgctgttt
     8581 ttataatatc taaatttatt ctatttcaat tcctttaaat aacttttacc gaactctggt
     8641 aatgttacgt tgaaattatc tgctatagtt gcaccgatag aactgaatgt agtatcactt
     8701 tctagtgcat gaccaccttt aaatttcgga ctgtacataa ttactgggat atattctctc
     8761 gtatggtccg tacctggcgc tgtcgggtca ttaccatggt ctgctgtaat aattactaaa
     8821 tcgtcttctt ttaagttgct aaacagttct ggcaagcgat catcgaaatc tttaattgct
     8881 tgtgcataac ctggtttatc acgacgatga ccgtataatg catcaaagtc tactaagttt
     8941 aagaagctaa tacctgtgaa atctttctta acaattttca tcaattgatc cataccgtcc
     9001 atgttactct tcgtacgaac cgcttctgtt acaccttcac catcataaat gtcattaatt
     9061 ttaccgatgg caataacatc ataaccaccg tctttcaaat gatctaagac agttttacca
     9121 aaaggtttta acgcatagtc atgtcgatta gatgtacgtg taaagtttcc tggttcacca
     9181 acatatggac gtgcgataat acgaccaatt aaatatttag ggtcttttgt caactcacga
     9241 accttttcac aaatatcata taactcttct aatgggataa tgtcttcatg tgcagcaatt
     9301 tgcaatactg ggtctgcact tgtataaaca attaagtcac cagttttcat ttggtgctcg
     9361 ccccactcat cgataatttg cgtacccgat gccggtttgt tagcaacaac tttacgacct
     9421 gtcatttctt caatttgttg aattaactct tcagggaatc cattagggta tactttaaaa
     9481 ggttgcataa tatttaatcc cataatttcc cagtgaccag tcattgtatc tttaccaact
     9541 gaagcttcac tcaatttagt atagtatgct tctggttgtt caactgcatt tactactggt
     9601 aatttatcga tgttccctag acctaacttt tcaaggtttg gtaaagtttg atcgaaacct
     9661 tctaaggtat gtcttaaagt atgtgaacct tcatctttaa aatcagctgc gtctggcgct
     9721 tcaccaatac ctactgaatc cattacgatt aaatgtacac gattaaatgg tcttgtcata
     9781 gctatcactc ccaaaattta tatatattag taatctgaat ctgcttctaa accttgcata
     9841 atttgaac
//
LOCUS       DALAAR010000088         9705 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000088 DALAAR010000000
VERSION     DALAAR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.090"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(111..383)
                     /locus_tag="O2458_002325"
     CDS             complement(111..383)
                     /locus_tag="O2458_002325"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ3888171.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            complement(644..796)
                     /locus_tag="O2458_002326"
     CDS             complement(644..796)
                     /locus_tag="O2458_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888172.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            complement(1320..1679)
                     /locus_tag="O2458_002327"
     CDS             complement(1320..1679)
                     /locus_tag="O2458_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCZ3888173.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(1698..2543)
                     /locus_tag="O2458_002328"
     CDS             complement(1698..2543)
                     /locus_tag="O2458_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ3888174.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            3015..3695
                     /locus_tag="O2458_002329"
     CDS             3015..3695
                     /locus_tag="O2458_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCZ3888175.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            3981..4676
                     /locus_tag="O2458_002330"
     CDS             3981..4676
                     /locus_tag="O2458_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCZ3888176.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            4967..6025
                     /locus_tag="O2458_002331"
     CDS             4967..6025
                     /locus_tag="O2458_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCZ3888177.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            6144..6281
                     /locus_tag="O2458_002332"
     CDS             6144..6281
                     /locus_tag="O2458_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888178.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            6390..7316
                     /locus_tag="O2458_002333"
     CDS             6390..7316
                     /locus_tag="O2458_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCZ3888179.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            7680..8384
                     /locus_tag="O2458_002334"
     CDS             7680..8384
                     /locus_tag="O2458_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCZ3888180.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            8830..9525
                     /locus_tag="O2458_002335"
     CDS             8830..9525
                     /locus_tag="O2458_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCZ3888181.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
BASE COUNT         3711 a         1393 c         1641 g         2960 t
ORIGIN      
        1 taaataaatt taaactattt cttagtctct ccatatcgtt ggaagttggt tctttatata
       61 atcagataaa ttagaatctt caagtaatgt atctttaaaa agttcagttt ctaaatcaac
      121 tttaaaattt gctttaaaac atcatgttcc atttttaaat gttgaacttc tttgcgtaat
      181 tttattaact ctttttcatc actttttaaa ttatcttgat gattgaaggg tccagtattt
      241 tggtgttgtt ttatcgaatt tgagataatt gagggtttta aatcatactt tcgtataatt
      301 tcattcttaa gcttaccatt tttatataat ctaaccattt gtaacttaaa ctctgaacta
      361 aatgttcttc tttcttttgt cataataaaa ttgactactt tcttaattta acaatatcta
      421 ttctcataga atttgttcaa ttaagtgtag acgattcact gacacatcat aagtacgtaa
      481 ggaattttga tcgttcattg ttattgcttt atgttttagg gaatgtgcta ttgaaggggt
      541 taaagatgtt tgaaatgtct tggtttcttg acttatatag ttatgaaaga gggcgttgtc
      601 ctttatagca tttctgagat tagaaatgct ataaataaag tgattaagat tttttgtttt
      661 catgtaattt aagaaatgga tttgtttttt gtgggttatt tttaattctg ttcttaaatg
      721 gtttgaattt tgcgattgca aatatgatag ctgcttcttt tgacaaagta atagaacctt
      781 tttcttctgg taccatgata atcactcctt gtgcaattgg atttaaatat tataaaataa
      841 ttttactata caaaatatat tgtgtaaata gtttcttatt gttaccaaat aatatgtttg
      901 gaagcgttat gtaatgccaa tcaacaccga agttctcatg tcctctttcc ttttacgact
      961 acatatattg ttaataatat tgaaataata aactgatagt gataatattt atttaactga
     1021 tccatgagag taggaacccc atcatacggg aatttcgaaa tcatgggttt tttgttttaa
     1081 aggattgatg caatagataa agtagccatt ctaatatatg gagtatttat cagctgaaaa
     1141 ttaaatcaga tatgggtttt cttcattaaa taatttttct ataaggaaac agaggcaacg
     1201 ctactgtcac ctctgcttga aataattagg atgaaatact ctgagtctaa agtccacaca
     1261 caattcgctc aaatcctcca aacctccgac tacatctcca cccccccaaa caccaatact
     1321 taccaaagca ttgatatgag tgccaatact ggcaatgtgc cttgtttgaa aaagatacta
     1381 atattgcttg aaaggccacc ataaatagca acgccaatga tatacactaa aatagctgcg
     1441 catatttctt ttggattact gctgataaac aaaccgtata ttagcaaaac tccgattaaa
     1501 ccgttatata cgccttggtt cttcaaaagt aggttaatat ttttgtcttt caatttatcg
     1561 acgcttatat taaatgtctc gctagtcttt ttggaagttg tagcaatcgt ttcaaggtac
     1621 ataatataga aaaactctaa tgccacaaat atgattaaaa ttgttgagat gatattcact
     1681 ataacgctcc tttattatta aatattttct tgtaaaaatg attgcagtgt ctgtggttga
     1741 tcattgacta attgttggaa atcattggat tcttggtcta atagtcctct tgctcctgcg
     1801 tcgtacattg atgccaataa tgcaccaaag cctttaggtt catcatacat ttctgcaaat
     1861 gtctctaatg aaacgggctc atatttaatt tctgtgcctg atgcctcaga taaaattgca
     1921 gcaagttctt tcatatcata actgtagcct gataataagt agcgtttgcc ccaagtatct
     1981 ggatttttaa taatagcaat gacacctcta gcaatatcat ttctagtaat ataattaata
     2041 cgaccatcgc cagctggata aatcagttta tgcatattca tcaattctgg taaatatggt
     2101 ttaagtggat ccatgtacat tgccattctt acatacgtat agtcaatgcc acttgttgcc
     2161 aatagacgtg ctgcataacc aaaataagga ctcatatgga atggattatt atgctgatct
     2221 gcgtaataac ctatgaaaat gatatgagca acgccgctct gctttgccgc atatactaaa
     2281 ttttccactt caggaatacg tttgaatgat ggatggataa tacttggaat aaacacaacg
     2341 gtatccattc ctttaaatgc ttctaccatg ctttcttgat taaaataatc taattgtcga
     2401 acaggaactt ttccgcgcca atcttctgga actttctcaa catttctaac accaatgtga
     2461 aaatgatcta tgtgatttgc aatggcttga tttgtaatat gtgtgcctaa atgacctgta
     2521 gcacctgtta acataatatt cattcacttc atctcctaat ctttatatac ataacataat
     2581 acttatttga tggttttcaa aacatttgat tttataaaaa attctaatct gtatttattg
     2641 tcgacgtgta tagtaaatac gtaaatatta ttaatgttga aaatgccgta atgacgcgtt
     2701 ttagttgatg tgtatcacta atatcattga aaattttaat caggtactac gacaatatga
     2761 tgtctgtttt gtgtctgaaa gttttacagt ttttaaaata aaaatggtat aaagtgtgat
     2821 ttgtataaaa aagagtctcg acggataaga attgattaat aacagttagc attttattaa
     2881 ttaccttaac aatgattcaa gtttagttaa atgaggttta atttgaaagg ggatagcgcc
     2941 tcaatataat gtaggtagat tgttcatatt acgtaattga aaaatcaaat ttaaatagat
     3001 tggggctaaa aattatgaaa tttaaagcga tagcaaaagc aagtttagca ttgggaatgt
     3061 tagcaacagg tgtaattaca tcgaatgtac aatcagtaca agcgaaagca gaagttaaac
     3121 aacaaagtga atcagagtta aaacactatt ataataaacc aattttagag cgtaaaaatg
     3181 tgactggatt taaatatact gatgagggta aacactattt agaagtcaca gtagggcaac
     3241 agcattctcg aatcacttta cttggatctg ataaagataa atttaaagac ggagaaaact
     3301 caaatataga tgtgtttatc cttagagaag gtgacagtag acaagcaaca aattactcaa
     3361 ttggtggcgt tacaaaatca aatagtgtgc agtatattga ttatatcaat acgccaattt
     3421 tagaaatcaa gaaagataat gaagatgtac ttaaagattt ttactacatt tcaaaagaag
     3481 acatctcatt aaaagaactt gattatagat taagagaacg tgcgattaaa caacacggct
     3541 tgtattcaaa tggtcttaaa caaggtcaaa ttacaattac aatgaatgat ggcacaacac
     3601 atacaatcga tttaagtcaa aaacttgaaa aagaacgtat gggtgagtca atcgacggca
     3661 ctaagattaa taaaattcta gtagaaatga aataatactt tctaacaaca aagcgctatg
     3721 ttgaatagtg cttgttatgg aaatatatgg aagttaagcg acgtactgtt gcttagcttc
     3781 tttttttgag gggaaaagtt acaaaactca cacaaacagt cgcaccacgc attatctttt
     3841 gcttaaatag cttaatcata ttttatgaat agttaaaaac aggttaatgt gaatatccga
     3901 atacagctcc tataatatgg gtgtatgatt caaattacgt aataaaacaa tctaattata
     3961 atagattgga gcatacaact atgaaaatga aaaatattgc aaaaataagt ttgttattag
     4021 gaatattagc aacaggtgta aacactacaa cggaaaaacc agttcatgcc gaaaagaaac
     4081 ctattgtaat aagtgaaaat agcaaaaaat taaaagctta ttataatcaa cctagtattg
     4141 aatataaaaa tgtgacaggt tatatcagtt tcattcaacc aagtattaaa tttatgaata
     4201 tcatagatgg taattctgtt aataatattg ctttaattgg caaagataag caacattatc
     4261 atacgggtgt acatcgtaat cttaatatat tttacgttaa tgaggataag agatttgaag
     4321 gtgcaaagta ctctattggg ggtatcacga gtgcaaacga taaagctgtc gacctaatag
     4381 cagaagcaag agttattaaa gaagatcata ctggtgaata tgattatgac tttttcccat
     4441 ttaaaataga taaagaagcg atgtcattga aagagattga ttttaaatta agaaaatacc
     4501 ttattgataa ttatggtctt tacggtgaaa tgagtacagg aaaaattaca gtcaaaaaga
     4561 aatactatgg aaagtataca tttgaattgg ataaaaagtt acaagaagac cgtatgtccg
     4621 atgttatcaa tgtcacagat attgatagaa ttgaaatcaa agttataaaa gcataacaca
     4681 tatacttgat gacgaaataa gttgaaattg aaatagagag gttaagtgac gatcaaacgt
     4741 tgcttaactt ctttttaatg cttaaaaatt atttcaaagg cacatagaaa cgctatatta
     4801 atctcatact cactcattat tttttgctta aattacttaa taatacttca ataattgtta
     4861 aaaggggttt aatgtgatta tcttagaacg ccatctataa tgatgttgta tgattcaaat
     4921 tacgtaaaaa gacaatcgaa tataatatag attggagcat acaattatga aaatgagaac
     4981 aattgctaaa accagtttag cactagggct tttaacaaca ggcgcaatta cagtaacgac
     5041 gcaatcggtc aaagcagaaa aaatacaatc aactaaagtt gacaaagtac caacgcttaa
     5101 agcagagcga ttagcaatga taaacataac agcaggtgca aattcagcga caacacaagc
     5161 agctaacaca agacaagaac gcacgcctaa actcgaaaag gcaccaaata ctaatgagga
     5221 aaaaacctca gcttccaaaa tagaaaaaat atcacaacct aaacaagaag agcagaaaac
     5281 gcttaatata tcagcaacgc cagcgcctaa acaagaacaa tcacaaacga caaccgaatc
     5341 cacaacgccg aaaactaaag tgacaacacc tccatcaaca aacacgccac aaccaatgca
     5401 atctactaaa tcagacacac cacaatctcc aaccataaaa caagcacaaa cagatatgac
     5461 tcctaaatat gaagatttaa gagcgtatta tacaaaaccg agttttgaat ttgaaaagca
     5521 gtttggattt atgctcaaac catggacgac ggttaggttt atgaatgtta ttccaaatag
     5581 gttcatctat aaaatagctt tagttggaaa agatgagaaa aaatataaag atggacctta
     5641 cgataatatc gatgtattta tcgttttaga agacaataaa tatcaattga aaaaatattc
     5701 tgtcggtggc atcacgaaga ctaatagtaa aaaagttaat cacaaagtag aattaagcat
     5761 tactaaaaaa gataatcaag gtatgatttc acgcgatgtt tcagaataca tgattactaa
     5821 ggaagagatt tccttgaaag agcttgattt taaattgaga aaacaactta ttgaaaaaca
     5881 taatctttac ggtaacatgg gttcaggaac aatcgttatt aaaatgaaaa acggtgggaa
     5941 atatacgttt gaattacaca aaaaactgca agagcatcgt atggcaggca ctaatattga
     6001 taacattgaa gtgaatataa aataatcatg acattctcta aatagaagct gtcatcggaa
     6061 aaacaagaag ttaagtgaca acggtttaca tgttgcttag cttcttttat tatgcgtaat
     6121 gatgtaaaaa gacgaatatt catttgtttg taaaagtggc atttctatgt cttaaaagtg
     6181 acgaaacttc aaatgtgcca agtgttgaat cacatcaaaa tcatttttat ttaacgaaca
     6241 ttatggattt cttaatttac ttaacgatga ttcaaatata gttaaacaag gtttaatgtg
     6301 aatggagcaa tacgccatct ataataaagc tgtatgattc aatgaatgta atcgaacaaa
     6361 tctaataatt acgaatggag catacaacta tgaaaataac aacgattgct aaaacaagtt
     6421 tagcactagg ccttttaaca acaggtgtaa tcacaacgac aacgcaagca gcaaacgcga
     6481 caacaccatc ttccactaaa gtggaagcac cacaatcaac accgccctca actaaaatag
     6541 aagcaccgca atcaaaacca aacgcgacaa caccgccctc aactaaagta gaagcaccgc
     6601 aacaaacagc aaatgcgaca acaccgcctt caactaaagt gacaacacct ccatcaacaa
     6661 acacgccaca accaatgcaa tctactaaat cagacacacc acaatcgcca accacaaaac
     6721 aagtaccaac agaaataaat cctaaattta aagatttaag agcgtattat acgaaaccaa
     6781 gtttagaatt taaaaatgag attggtatta ttttaaaaaa atggacgaca ataagattta
     6841 tgaatgttgt cccagattat ttcatatata aaattgcttt agttggtaaa gatgataaaa
     6901 aatatggtga aggagtacat aggaatgtcg atgtatttgt cgttttagaa gaaaataatt
     6961 acaatctgga aaaatattct gtcggtggta tcacaaagag taatagtaaa aaagttgatc
     7021 acaaagcagg agtaagaatt actaaggaag ataataaagg tacaatctct catgatgttt
     7081 cagaattcaa gattactaaa gaacagattt ccttgaaaga acttgatttt aaattgagaa
     7141 aacaacttat tgaaaaaaat aatctgtacg gtaacgttgg ttcaggtaaa attgttatta
     7201 aaatgaaaaa cggtggaaag tacacgtttg aattgcacaa aaaattacaa gaaaatcgca
     7261 tggcagatgt catagatggc actaatattg ataacattga agtgaatata aaataatcat
     7321 gacattctct aaatagaagc tgtcatcgga aaaacaagaa gttaagtgac aacggcctac
     7381 atgttgctta gcttcttttg ttatgttcga tgatttgaga acccgaattt tcgatgggtc
     7441 caaatatgac gtggaagaga cctgaattta tctgtaaatc cctatctatc gggtgtgaag
     7501 cacaacggga tcagttttat ttaacgaaca ttatagattc cttaatttac ttaataatga
     7561 ttcaatgatt attaaacatg gtttaatgtg aaaggtcaaa tacgctaact ataataaagc
     7621 tgtatgattc aatagacgta agcgaacaaa tctaataatt acgaatggag catacaacta
     7681 tgaaaatgac agcaattgcg aaagcaagtt tagcattagg tattttagca acaggaacaa
     7741 taacgtcatt gcatcaaact gtaaatgcga gtgaacataa agcaaaatat gaaaatgtga
     7801 caaaagatat ctttgactta agagattact atagtggcgc aagtaaggaa cttaaaaatg
     7861 ttactggtta tcgttatagc aaaggtggca agcattacct tatctttgat aaaaatagaa
     7921 aattcacaag agtacagata tttggtaaag atattgaaag atttaaagca cgtaaaaatc
     7981 cgggattaga catatttgtt gttaaagaag cggaaaaccg taatggcaca gtgttttcat
     8041 atggtggtgt cactaagaaa aatcaagacg cttattatga ttatataaac gcaccaagat
     8101 ttcaaatcaa gagagatgaa ggtgacggta ttgctacgta cggtagagta cactacattt
     8161 ataaagaaga gatttcactt aaagaactcg actttaaatt gagacagtat ttaattcaaa
     8221 attttgatct gtataaaaag tttcctaaag atagtaagat aaaagtgata atgaaagatg
     8281 gcggctatta tacgtttgaa cttaataaaa aattacaaac aaatcgcatg agtgacgtca
     8341 ttgacggtag aaatattgaa aaaatagaag ccaacattag ataattcaat gaaatatgga
     8401 taatagtaaa atatggatag tatagaggag ttaggcaaca taagttgctt agcttctttt
     8461 ttgtgttgga gagatgaaaa tgaagcgtat cgatgaataa taaaaacacc aataaaactt
     8521 gtggaaatag ttgatactta tagtcgcgcg ttgtcctttt cgtgacatga aacaatgtgg
     8581 aaaacatagt taaattgagg gaaagtgtga atagttaaaa aagctgcgtt aagtttaaaa
     8641 aatagattaa cgctgttagg attccattaa ttagcttaac attggttcaa aaatagttaa
     8701 aaagaggtta attcatagct tagtattacg cttatataat gatagtagat tgttcgtatt
     8761 acgtaattga aataatcata taaaaatata ttaagacaaa atttataaat agattgggag
     8821 aatagtacta tgaaattaaa aacgttagct aaagcaacat tagtattggg attgttagct
     8881 actggtgtaa taacaacaga aagtcaaaca gtaaaagcgg cagaatcaac tcaaggtcaa
     8941 cacaattata aatcgttaaa atactactat agcaagccaa gtatagagtt aaaaaatctt
     9001 gatggtttgt atagacagaa agtgacagat aaaggagtat atgtttggaa ggatcgaaaa
     9061 gattattttg ttggcttgct tggtaaagat attgaaaaat accctcaagg tgagcatgat
     9121 aagcaagatg catttttagt catcgaggag gaaactgtta atggaagaca atattcaatt
     9181 ggtggtttaa gtaagacaaa tagtaaagaa tttagtaaag aagtcgatgt taaagtaaca
     9241 agaaaaattg atgaatcatc ggaaaagtct aaagatagta aatttaaaat tactaaagaa
     9301 gaaatctcgt taaaagagtt ggactttaaa ttaagaaaaa aattgatgga agaagaaaaa
     9361 ttatatggtg ctgttaataa tagaaaaggt aaaattgtag ttaaaatgga agatgataag
     9421 ttttatactt tcgaacttac aaaaaaacta caaccgcatc gcatgggtga cacgatagat
     9481 ggtaccaaaa tcaaagaaat taatgttgag ctagaatata aataatcttt ggacaagcag
     9541 actagtaatt gtagggaagt taagcgataa catattgctt agcttctttt ttattttgtt
     9601 atgatgaaaa aaggagcggg tttatgatca agtttttgga aaaacggttg atacttatag
     9661 tcgcgcgttg tccttttcgt gacatgaaac aatgtggaaa acata
//
LOCUS       DALAAR010000089         9646 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000089 DALAAR010000000
VERSION     DALAAR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.091"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            73..216
                     /locus_tag="O2458_002336"
     CDS             73..216
                     /locus_tag="O2458_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ3888182.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            281..3274
                     /locus_tag="O2458_002337"
     CDS             281..3274
                     /locus_tag="O2458_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCZ3888183.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTE
                     IGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSR
                     NNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQK
                     FLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVIS
                     PLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRN
                     HNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNN
                     MYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYWKINE
                     MVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEYKKHK
                     RVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHI
                     DKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYSI
                     LKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFK
                     FDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANN
                     FEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKD
                     QETFVLSIVKEIVKTQKHWCGDKND"
     gene            3267..4511
                     /locus_tag="O2458_002338"
     CDS             3267..4511
                     /locus_tag="O2458_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCZ3888184.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            4527..5045
                     /locus_tag="O2458_002339"
     CDS             4527..5045
                     /locus_tag="O2458_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCZ3888185.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            5055..6428
                     /locus_tag="O2458_002340"
     CDS             5055..6428
                     /locus_tag="O2458_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCZ3888186.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            6451..7143
                     /locus_tag="O2458_002341"
     CDS             6451..7143
                     /locus_tag="O2458_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCZ3888187.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            7140..7901
                     /locus_tag="O2458_002342"
     CDS             7140..7901
                     /locus_tag="O2458_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCZ3888188.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            7898..8596
                     /locus_tag="O2458_002343"
     CDS             7898..8596
                     /locus_tag="O2458_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCZ3888189.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(9070..9636)
                     /locus_tag="O2458_002344"
     CDS             complement(9070..9636)
                     /locus_tag="O2458_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ3888190.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
BASE COUNT         3542 a         1216 c         1613 g         3275 t
ORIGIN      
        1 tgttgtgtga aaagggataa tatgacaatt gaagtaggcc ttaacttcaa ctaataatag
       61 gaggttttat ctatggaaaa agttcttgat ttagacgtgc aagttaaagc aaacaataac
      121 tcaaatgatt cagcaggtga cgaacgtatt acaagtcata gtttatgtac tcctggttgt
      181 gctaagactg gtagttttaa tagcttctgc tgttaagatt acagcaaata gagcctgaaa
      241 gggctctatt tgtctattca aatatgtgag gtgcttacaa atgaacacta tttacgaacc
      301 gtcaagtatt tgtatgatta gaacaccttt attatcagtt gaatttttca atttattttt
      361 aaatactgaa caaattaaat atagcgactt acaattaaat gctcagatga aagaatcaat
      421 cctaacaaca acatttaact tatattgtac tttacaagaa ataaattttg atggcgataa
      481 caagaaggtt agagatgcta aagaaagttt attaaaatat ttaattagaa tgtcaacacg
      541 tccaactcca tttggattat taagtggtat taatttgggg cattttgtaa atgaaccaac
      601 acgtcttaaa gttggaaata gtattcaaaa atatgtgaaa gtcgatggcg aatggttgta
      661 taaactggtt agttatattg agagtattga tgaatattat caaaatttga aagttatttg
      721 gaatagtaaa gcacatatta tcaatgatcg aatatattta aatgaacaaa gtgctattta
      781 tttaaacaac aataaagata catctttttc cattaaaaat agcgaattac tcgtattcat
      841 caaaaccact gtgacgaata ataatattac tttttcaaat ttagctgaaa agataaatca
      901 agaatttgaa attaatgata ttactaaagt gaaagtatat atccataatc ttgtctcaaa
      961 ggaaattata tattcaacaa tcagaccacc gttatcatat agcgataatt taaattatat
     1021 tttaaataaa ctttctttac ataatgatga ttttgtgaaa aaaattagag aaatccagaa
     1081 attgatatta gcttatgaaa aaactgaaat tggatttggc gaggaattat ataaagacat
     1141 tattcatcat atgaaggcac tattcaaatg taaaaactat cttcaaattg ataccaaaat
     1201 tgacatgatt aacaattatt tacatcaaga tatagcaaca aatatatctg aagcagcgta
     1261 cttattgtgg cttttatcaa gaaataatat tggattcaca gatttgaaag ttcttcacaa
     1321 ccgttttatt gaaaagtatg gatttgaaca actcgtgaat attaaagatt tgatttcaga
     1381 tataacaggt ttcgggacaa caatttttca agaagaagaa accgatggaa ataatattgt
     1441 aatgcttaag caaaaatttt tacatgcctt gagaaataac gatgagatag tcataaatga
     1501 aaaagatgta gagtcattga ttaacgataa tgaaatcaat cattatcatg cgccaatgag
     1561 tgcagatgtt tatgctgaac tttatttagg acgtttttac aatcagtaca atgagctcat
     1621 tgttattagt cctttaacgg cgtcttttaa tgctggcgct acatttgggc gatttcatca
     1681 tttaattgat actgaaactt tagcaaaatt agaacatgaa aaaggacatt attatcagaa
     1741 gatgatatgt gatgacaatg tagaaatgat ttctataaat aacataccga aatatccgag
     1801 aaatcataat gtattaacta atcatgactc atacgaatat tcattgaatt taggaagtag
     1861 taatagttat tcaaagtatg agcttacctt agatgatatt tatgttggtg ctacctttaa
     1921 caaattatat ttatattcta gccaactaaa taaaagggta ctatttgaat caaacaatat
     1981 gtataacttt ttaaaagaat gtaatttata tcgattgtta cgagaaattt caatggaatc
     2041 tgtgaaatgc attgaaccta tgaatgatgt gagtattgat tcatttagtt attcaccacg
     2101 aatcagatat aaaaatgtca ttcttaaacc agcatattgg aaaattaacg agatggtatt
     2161 acctttacct aaaaatgaag aatgggatca acaatttttg aaatatcaag aacaattcaa
     2221 tatacctaat atcgtgaatt tagtttatgg agataacaag cttctactaa atttatcatt
     2281 agctaatcat agatatttat tgatgaaaga atataagaag cataaacgtg tacgtttagt
     2341 agaatcattt ttacctcaat caaagaatga tcatgtgtat gaaattgtta caccaattta
     2401 taaaaaatca tcttatcgag gacccgaaat cgaaattcca aaatataaaa atacagatat
     2461 tgaatatgac aaagactggt ttgcattaca tattcatatc gataagccat cgcaagatac
     2521 atttatcatc gacaatttgt atccatttgt aaaacatctt aaagataagg gagatataga
     2581 tcaatatttt ttaatgcgtt atatcaaaca aggtgacatt ttaaagttga ggttattcag
     2641 gaatgacgaa aattacgcag aaatatacag catcttgaag aattggttac cacatgttcg
     2701 acaaacgaca gaagtttcag attatgaatt tgtatcatac gagccagaat tttttagata
     2761 tggtggaaag aacacgatta atgaaataga agccttcttt gaatatgaca cgaatttagc
     2821 tgtcaatatt attgaaaatg attttaagtt tgatcgacca tatattgttg ctatttcaat
     2881 aatgtattta tttgagatgt tttcaatttc taatgaagag cgtatggaaa ttgttaataa
     2941 ctatgtacct acatcattta aaagtaaaga tattagacct tttaaaaatg aacttgtaac
     3001 tatatgtaat cctgctaata attttgaata tatggctaaa cactatagtg gtatttatcg
     3061 aattttaaaa gatggcaacc aaattttgag caaattaaat gaagggctta aaaaaacttt
     3121 aactactaaa aggtcaagga ttattggcag cttaattcat atgcgctgca atcgaatatt
     3181 tggcattgat aaggatcaag aaacatttgt tttatctatt gtaaaagaaa ttgttaaaac
     3241 tcaaaagcat tggtgtggtg acaaaaatga ttgatatgaa tctaatattg aagaagaagt
     3301 ttcaagagat ttcagaagta gatgatttca tttcaaaagc atctactgaa accgattatt
     3361 ttgaaccttc aactttatca catggtatac caggtatcat tttgttttta gatgcttatc
     3421 aaaaagtttt cgatattaat actgagcaaa ttgtgcataa gtacatcatg aaattagcac
     3481 catatttaca gagtggtcaa tataacaatt ctttgtttgg tggactttca ggtattgcat
     3541 tttcaatgga tttagcatcg caaaatggca aaaattatca aaatatttta aataatattg
     3601 atggtcacat tgtaaatgaa atagaaaata aatttgatca aatattacaa gaaccattaa
     3661 atccattaaa ttatgatact gtcagtggat tagctgggat agggagatat ttgctaaata
     3721 gagtagatga gaatgaattt aatgttaaag cattaaaaag catattagta tactttaaag
     3781 atattcaata ttctaaaaat agctgggtag tcccacaaga aagtcaattt ttagagtctg
     3841 ataaaaatta ttttactgaa ggtaatatca atcttggcct tgcacatgga gtgctaggac
     3901 cgatgtcttt atttgcatta tgcgtgatta aaggaattac gattgaaaat catcagcaca
     3961 tattaaaaga catgtacaaa tttatcatgg acgaaaaatt ttgtaaccac gaaagatggt
     4021 tgcagcgtta cgatttaatt tctgaacgta atcatttcaa ttttattcgg aatggttggt
     4081 gttatggcaa tacgggtgta atgacgacgt tgtttttaat cggccaagca ttacaagatg
     4141 atgaaataat taaaatgtct aaaaaagtga tgctacaagt agtaaatgat aaagatgaaa
     4201 atttaataag tccaactatt tgtcatggat tgtcatcaca aatattaatg ttaacaatta
     4261 tgaatttgaa ttttgaatta aatgaagtgt ctgattatat cactgtatta ataaataaac
     4321 tgatttctca ttataaggaa gattatctgg tgaattttat agacattaat gaaaataagc
     4381 aagatgtatt taaaagtagg aaagttggcc ttttagaagg tgaattaggg gtctatctaa
     4441 cattgatgac actgaaaaat acaaaaattc tcaatgaaaa aaattggacc aatgcgtttt
     4501 taattagctg aatggaggcg tatatcatgg gagaaaatgt attgatttgt ttgtgtggct
     4561 cagttaatag tatcaatatt agccactata ttattgaatt aaaatcaaaa tttgatgaag
     4621 taaatgtaat cgcgtcaaca aacggtagaa agtttattaa tggtgaaata ttaaagcaat
     4681 tttgcgataa ctattatgac gaatttgaag accctttttt aaatcatgta gatatagcaa
     4741 ataaacatga caagattatt attttacctg cgacttctaa tacgattaat aaaattgcaa
     4801 atggtatatg tgacaattta ttattaacta tttgtcatac agcttttgaa aaactttcta
     4861 tatttccaaa tatgaattta cgaatgtggg aaaatccagt tactcaaaat aatattcgat
     4921 tattaaaaga ttatggtgta tcaatatatc cagcaaatat ttcagaaagt tatgaattag
     4981 cgtcaaaaac atttaaaaag aatgttgtcg cacctgaacc atataaagtt ctggaattca
     5041 tttgagatta gaatatgaaa atcataaagc gcgctattat tagtttaata atattgagtc
     5101 ttttaatatc tattacaatg agtaatgcat cagcgtcaga agaactatat tacagtgttg
     5161 aatacaaaaa tacagcaaca tttaacaagt tagttaaaaa gaagtcctta aacgttgtct
     5221 ataatatacc ggaattacat gtggcacaga ttaaaatgac gaaaatgcat gctaatgctt
     5281 tagcaaacta taaaaatgat attaaatata tcaatgccac atgttcaact tgtattacta
     5341 gcgagaaaac aatagacaga acatcaaatg agtcattatt ttcaagacaa tgggatatga
     5401 ataaaataac caataatggt gcatcgtatg atgatttgcc aaaacatgct aacaccaaaa
     5461 tagcaatcat agatacaggt gtgatgaaaa accatgacga tttgaaaaat aatttctcga
     5521 ctgattctaa aaatttagta cctttaaacg gttttagagg tactgaaccg gaggaaacag
     5581 gtgatgttca cgatgtcaat gataggaaag gacatggcac gatggtgtcg ggtcaaacga
     5641 gtgctaatgg taagttaata ggtgttgcac cgaataacaa atttacaatg tatcgcgtgt
     5701 ttggtagtaa aaaaacagaa ctgctttggg tatcaaaagc gattgttcaa gctgcaaatg
     5761 atggaaatca agtcattaat attagtgttg gtagttatat tattttggac aaaaatgacc
     5821 atcaaacatt tagaaaagat gaaaaagtag aatacgatgc gttacagaaa gcaatcaatt
     5881 acgccaagaa gaaaaaatct atcgttgttg ctgcagctgg taatgatggt attgatgtca
     5941 atgacaaaca gaaactaaaa ttacagcgtg aatatcaagg taatggcgaa gtgaaagatg
     6001 ttcctgcatc tatggacaat gtcgttacag taggatctac agatcaaaag agtaatctat
     6061 ctgagttttc caattttggt atgaattata cagatattgc tgcgcccgga ggatcatttg
     6121 cttatttaaa tcaattcggt gtggataaat ggatgaatga agggtatatg cataaggaga
     6181 acattttaac tactgccaat aacggaagat atatttatca agctggaact tcattagcca
     6241 cacctaaagt ttcgggagca ctagctttaa tcattgataa atatcatctt gaaaaacatc
     6301 cagataaagc gattgaattg ttatatcagc atgggacatc taagaataat aaaccattta
     6361 gtagatatgg gcatggtgag cttgatgtgt ataaagcatt aaatgtagca aatcaaaaag
     6421 caagttaata aatcaaagga gtttttgatt atggcaaaat tagttactga aaacatttcg
     6481 aagcggttta aaaatcaaga tgtattaaag catattaata tcactttaga aaataacgaa
     6541 gtttatggat tacttggtat taatggagcc ggtaaaacga cacttatgaa aattatatgt
     6601 ggcatacttc aacaagattc aggggaaatt aaattagata atagaccaat gacacgaaat
     6661 gatttgcaca aagttggttc gcttattgaa acacctgcga catataatca tttaagtgca
     6721 caagataatt tgaaaattgt gtgtttaaat gaaagcgttg atttcagcga aattaatagt
     6781 gttttaagct tagtcaattt aaatgtcgat aaaaagaaaa aggttaagga cttttcttta
     6841 ggtatgaaac aaagacttgg aattgcaatg gcattaatta aaaagccaga aattttagta
     6901 ttagacgaac catctaatgg tttagaccca tatggaatcc aagaacttag agaacttcta
     6961 aaattattaa cagaacaagg tactagtatt attatttcaa gtcacatttt atctgaaatc
     7021 caagttttag cagatcatat cggtattatt catgagggtg agctaaaata tcagcaaaga
     7081 aataacaaag atgaaaactt agaagagata ttcttcaaaa taacgaaagg tgattacaaa
     7141 tgatacattt aaagatagaa ggtatcaaat ttaaaaattc tttcagtatg tatgttttat
     7201 taataagtcc gctggtattt ctttgttttg ctattttcac agtcttattc gccaaaagta
     7261 atacgggaac agcgaatagt gtgtcaccat atataacttt actatttaat atttggccaa
     7321 ttgctttcat cccgattgta ttatgtatgg cttgtaattc gttatttaaa attgaaatga
     7381 gaaataaatc atttaattat tacttaagta ataattggtc gattacaaaa gaaataagag
     7441 caaagatttt cattttatca atagcatttt tggtacattg ctttttagta tttattattg
     7501 cttatatagg tgacctaatt attaatccac atccggttaa tgctatgttg ttattggtga
     7561 caatattgtt gatgtatgta gtatctctac cattgatacc gctcaacttt ttattaactc
     7621 gatactttgg tgtgttcgta tcaatattaa taaacttagt attatcagtc atttgtgtct
     7681 tgtttttaac attgaagagt ttattttggg tgttgccgtg ggggataatg cagagaatcc
     7741 cgcttattac gcttggtata ctacctaatg gtttagttgt aaaccataat tcaaaatact
     7801 ttaatgatct caatgcctta tatatttcga ttattgttag catcattatt ttcgcgatag
     7861 taacattttt aaataataag aaaagttggc gattaaaatg ataattaacg aattaaagtc
     7921 atgtaagttg aaattttcta agcaagcgct cacatttgta cccattattg taaccatatt
     7981 gtttatatta tttataaatt ggtatttaaa cgtaaattta tggaatggtc gacaaataag
     8041 tttgtttaca gcgagtttta atgcaattac atcgctatta atttctataa acgtctatca
     8101 agttatcaat tttgaagaaa atattggtca ctttaatcat attttaggaa aagctaatag
     8161 gctaaattgg ttaaatgcat caatgatttt tacttatact attacagcca tatgtattct
     8221 attagcatca attaatttat tgtggcattc acatgatatg aaaataacac ttatgtttat
     8281 aggcgtatca ttgtttttca atgtaattat attactgcta ctttttattt ttagtatttt
     8341 cattaaagat gtaatggcta ttgttgtcgg agttttaatg tttattttta acgtttattt
     8401 tggattagaa gtgcttggag atcattcgtg gttctattta ccaatcacat atgctacacg
     8461 ttacgtttat atgtttatcg aagggagtat accagttaca ttaacattgg caatctatat
     8521 tattatcacg ttgctattag cagttttact ctttaaagga tttaataaat ggagcggtag
     8581 aacaattaaa gattaaagat tggggctcta acggtaaaag aaatttagtt gcgttgaaaa
     8641 gggaggaaag agaacagtta aatatgaata caaaataatt ttgttttcgg acagcagggg
     8701 tattagacgc gattgacaat gtctgtttaa ttaaacgtaa tgtttattta agcgatgaat
     8761 attaggtgaa aagtttttga atttgaatgt aattgaggtt tattgattag acattttatt
     8821 gaattgcgtg ttattatata aatgtaaaaa taagacgaca tgcgcgaaca tgtcgtctaa
     8881 gcaagccaaa cgctggcttc taaaatattt ttttaaccat tccaacttgc caaagttaat
     8941 gcggaatggt ttttttattt tccgctaatt gaaataaaaa tgacgtttta atatattatg
     9001 ggctaggtgg tttgtaagaa agggttagtt attaatgttt tatgaattaa ggaaatttga
     9061 gtttaaggtt taatcaattg tgattttgtt gatgaagcgt ttagttagag tattttcgcc
     9121 accactagtt actccttctc ccactttacc cgagactgga gaagagctat ctgaagaata
     9181 aatagatact ttttggccat tttgtagtaa accaagacct tttaacttct cggttagaga
     9241 attccatgta tttttagcat ctagcttttt gttaaagtca tcgtagacat tttccttagt
     9301 taaatcaatt tgttttaatc ctttaaagtc tatagattgt gttagatggc ctccatcatc
     9361 atttccagga gcagaaacgc tgctagaata tccattgctt aataaataag taacgttgat
     9421 tgtttcgtac tcgttactta aaataatatc agaatcatgt aatgaatctt taactttttt
     9481 ccataattga ccatctgtca ttttttcttc tgctttagcc gttttaacaa ctttatttgt
     9541 atctaatcct aagtatgaag aatgtaagcc tgttcctaat gttgttaata ctaaagcact
     9601 tgctactaat gttttaccta aaaattttgt attcattttt attgct
//
LOCUS       DALAAR010000090         9624 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000090 DALAAR010000000
VERSION     DALAAR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.092"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..184)
                     /locus_tag="O2458_002345"
     CDS             complement(<1..184)
                     /locus_tag="O2458_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ3888191.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMT"
     gene            complement(917..1282)
                     /locus_tag="O2458_002346"
     CDS             complement(917..1282)
                     /locus_tag="O2458_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ3888192.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(1424..1846)
                     /locus_tag="O2458_002347"
     CDS             complement(1424..1846)
                     /locus_tag="O2458_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCZ3888193.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            1980..2720
                     /locus_tag="O2458_002348"
     CDS             1980..2720
                     /locus_tag="O2458_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888194.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2713..3936
                     /locus_tag="O2458_002349"
     CDS             2713..3936
                     /locus_tag="O2458_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888195.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(4060..4563)
                     /gene="traP"
                     /locus_tag="O2458_002350"
     CDS             complement(4060..4563)
                     /gene="traP"
                     /locus_tag="O2458_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCZ3888196.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            4726..4836
                     /locus_tag="O2458_002351"
     CDS             4726..4836
                     /locus_tag="O2458_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888197.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            4826..5863
                     /gene="hemE"
                     /locus_tag="O2458_002352"
     CDS             4826..5863
                     /gene="hemE"
                     /locus_tag="O2458_002352"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCZ3888198.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            5921..6844
                     /gene="hemH"
                     /locus_tag="O2458_002353"
     CDS             5921..6844
                     /gene="hemH"
                     /locus_tag="O2458_002353"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCZ3888199.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            6868..8268
                     /locus_tag="O2458_002354"
     CDS             6868..8268
                     /locus_tag="O2458_002354"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCZ3888200.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            8591..9145
                     /locus_tag="O2458_002355"
     CDS             8591..9145
                     /locus_tag="O2458_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCZ3888201.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT         3371 a         1343 c         1759 g         3151 t
ORIGIN      
        1 acgtcataaa tacaagtaat aaaaagatga gaaaatgtct atctgataat ggcgtattag
       61 ataaaaaata cattacttca taactaacta aaaacataat gatggttgca actgcactca
      121 taaatgcaat tctaggcaaa ccaaatcgtg cattgatatc aatttgtctt ttacataaaa
      181 acatatctta cgcctcatct cttttcaata taagttagat tataactaac tcaggttcag
      241 taattcaata gtaacatatc atgttagaag aattgttata taagaagaaa ggagcgacgt
      301 attgtttgat ttgaaacgcg aaagtgggga ttggtaaaat tgtctgattg actagcgtag
      361 aagtagaatg cagaacaaat ttctgcgaac acatattctt gctaataaag catcacttat
      421 gaaaaaggcg cgtgtgacat gagtatttga aaacttgaat aatataaaaa atggatatgg
      481 cagggcatat gcaaaagcat cttttgacca actaagttat acaaatgcta aaaatatagt
      541 gatatgtaat tataataaac accagattaa taatttgtgc aagtaaggta tatgtaatgt
      601 gtatataagg ttcggctcaa tgaaaatata agtatgttgt ttgtcgataa tgatttaagt
      661 aaagcgtaat caaagtgcaa ggataacaca atgaatccta agcaatgttt tcaatcaatt
      721 cataaatatg atttaagcga aaatcactac aaacaatgtc ttcctataat ttgtaaacat
      781 aagttcattt gagttttaaa aaagagatga caagaaatat ctaatttcta atgtcatctc
      841 gtttatttgg catttaattt taaaaaataa atatgctgtt actaataaaa atagttgtga
      901 tcgagaacgt aacctgctac ttagaaattt cgtttccaat atcctcgcca cgattttgta
      961 aattttcgat gtgtgactgt aaacgttcaa tattaggatt aatgtcagat ttgaagtttc
     1021 caagtaatga cttaatttca ccaccaattg aactaccaaa ttccgcactt tcgtttttaa
     1081 tttgaacacc atagtttaaa atgtcattaa aactttgttt aatcgtttca aattcacgtt
     1141 gtagttctga ttttgaacca gtaggggcag tacgatcgat cgtgttgttc ttgacacttt
     1201 tgtcgtcacg tccttgaagt gcaactacaa aaccagctgc tacgccaaca ccgataccga
     1261 atagaatgcg tgatgctttc atttatattc tcctcacttt taacatctgt tatcttgata
     1321 actcattttg tttaaaattg ctaaccttga tgttatcact tcaaacgcaa ttgttataat
     1381 attaatcata ttatacccgt tcgagcttag attaagcata catttaaaat tgtgcttgaa
     1441 tttgttcagc aatttgttgt tttgcatcgt tatctaaaat gtcttcatgt gtttcccact
     1501 tataaccaaa tccatcaata tcattttcgt atcgaggaat taagtggaaa tgaatatgaa
     1561 atacagattg atctgcaaac tcaccattat tttgaataat gtttaaacca tcaggattaa
     1621 atgcacgctt aatagcattt gctactttag gtaatgctgc accgatatgt ttcattgttt
     1681 cttcatcagt ttcaaagata ttagcagaag cttttttagg aattaataac gtatgtcctt
     1741 tagtaacttg tgatatatct aaaaaggcat agacataatc gtcttcatat actttaaagc
     1801 taggaatttc tccagttaaa attttgccga aaattgtttc tgacatgatg actacactcc
     1861 ttttcaacta gtattaccat tcattataac attgtcgcta aaaattcgat attaggacat
     1921 gtgtatttaa tatgagcatt ttataatttt tggtacaata taagtcatgg aggtgcctta
     1981 tgacagttaa agtagaacag cttacaggtg gatatggaaa acgccctgta attaaagata
     2041 taaattttga attaaacaaa ggcgaaattg ttggacttat tggccttaat ggcgctggta
     2101 agagtacaac gattaaacat atgctaggat tgcttacacc tatggaagga tctctatcaa
     2161 tttcagatat caacattaat gatgacatag aggcttatag aagaaagtta tcttatattc
     2221 cggaatcacc ggttatttat gaagaactca cattagagga acacattgag atgacagcaa
     2281 tggcatatga tattgatcgt gatgaaacga tgaatcgagc aatgccatta ttaaagacat
     2341 tccgtttaga aaatgagttg aaagtttttc cgagtcattt ttctaagggg atgaaacaaa
     2401 aagtcatgat tatttgtgcg tttatcgtta atcccgaatt gtatattata gacgaaccat
     2461 tccttggcct agatccatta ggaattcaat ccatgttaga tttaatggtt gaaaagaaaa
     2521 acgaaggtag aaccgttcta atgagtacac atattttagc aacagctgaa cgttactgtg
     2581 atcgttttat catactagac gaaggtgaag tcgttgcatt tggagattta gaagcattga
     2641 gacaacaaac tggcttacat aatcaaacat tagacgatat atatattcat gtgacgcaag
     2701 gtggagatgt acatgcgtaa ccaagctacc acattattta ataaaagatt acatgccctt
     2761 cgtaaagaga aaaattatta taataagttt atatttaatg gtcatttcat ggtcttttta
     2821 cttattttgc tgggtgcatt tatttttgga tatggtgaat ggttaaaaca tattccaacg
     2881 aatatcaatt tttcattaat agcagcagtg attgttgcgt tgacatctat ttttccaatg
     2941 cgaccactgt taaaagaagc ggataaaatc tttttattac catttgaaaa acacatgtct
     3001 caatttatgc gtcatgcgat tttatatagt tactttgcgc gcatattaat ccaactcatt
     3061 attgttattg taatgttccc gttgttttac aatattaatc aacataatgt ggcattttat
     3121 atttgttttg gagtcagtgc attaattttt ccatatgttg gtttacgctt aagatggcaa
     3181 tggtatcaat cgggacttaa aacatggcaa gttaatttaa tatcattcat cacctttgct
     3241 ctaacatatt acttactatt agctccaaaa tggtacatcg catttgtgat ggttgctttg
     3301 cctgtgttaa tagaattttt agttaaaaaa tataaaccag gctttttgta tccttgggag
     3361 aaaatgattg caatcgaaca tcgacatcat atgaattatt ataaatttgt taatatgttc
     3421 accgatgtaa aacatttgaa agaatctgca gtcagaagaa gttatctaga tatactttta
     3481 ccagttccaa agggtagtaa atttaactct aatgcgatgt atttattctt atttattaga
     3541 agctttataa gagggagaga cgcatttaat attatcttta ggttagtaat tattgcagtt
     3601 ttattaatgg tttggttatc atatccatta gtgacagcaa ttattgggtg tttatttgta
     3661 tatattatat tgttacaaat ggcacaattt tattcacagc aagcatatgg cttatggcct
     3721 caagtgtggc ctgtacctga agagaaggta atcaaagggt atgaacagtt tttatataga
     3781 ttaatgtttg ttatttgtac tgtgtttgca gtgacattta ttataaaaca tatgacgtta
     3841 ttctatgttg tactcatttt ctatattgta ggtttattaa cgattagaag tattattaaa
     3901 aagttgaaat atcaggaaac attattacga gactaaaaaa cttccattgg catgtatgtt
     3961 gtaaaggtgc atgtaatgtt gaacgccaaa tgatacggcg ttcagattac attagcatct
     4021 atacgttaac agcataacca atggaagttt tcttcgaatc tattctttta ttgggtatag
     4081 atatctttca aaataacttg aatgttgtcc gcttgaacca aagtaatgac ttaatgcgtc
     4141 ttttgaaaaa tggtcattaa aggcatcaga ttgcttaaag tcttcgtatg catgtcgatc
     4201 agcaaatccg aaataaattt tgtatgttgt acctttagca ggtcttaaca aacgatagct
     4261 tttaaagcca ccaaagtttc tgaaattatc gtctacacta atcagtttct tttcaagttg
     4321 atatgcatga tcttctgttg atggaatgaa gattgcacaa tagaaatgat gttcactgaa
     4381 ttcaccaatt tctttaataa cttcatatat tgaaggtgat tttaaaacag tctcaccatc
     4441 agtttcttca aaaataactg aagtatctga tgctgaaaat tggaatagtt gatgggtcgg
     4501 gttattgatt tttatctgat gtaaaaatcc ataagtgcca taagatgtat atagtttctt
     4561 catatgaaag gtccccctta tgtttttatt tattatacga tgataaacta gtcattacca
     4621 ctattaataa ttgattaaaa ttactataat gaatcttgcg agatatttag aagtaatgat
     4681 acaaaataat ggactttaga atttaaattt acaataaata gttctatgat taaatgtcag
     4741 ttttatgaca tttatttatt gaaaatacga acgaatgagc gatatgataa tatagataag
     4801 aatgatttta atttaggagg cctttatggt gcataataaa aacaatacaa ttttaaaaat
     4861 gatcaaaggt gaagaaacat cacatacacc tgtttggttt atgcgacaag ctggccgttc
     4921 gcaaccagaa tatcgaaaat tgaaagaaaa atattcacta ttcgatatta cacatcagcc
     4981 ggagttgtgc gcttatgtaa cacatttacc agttgataat tatcatacag atgcagcaat
     5041 tttatacaaa gatattatga caccattaaa gccaattggt gtcgatgtag aaattaaatc
     5101 gggtattggt ccagtgattc ataatccaat caaaacaatt caagatgttg agaaactttc
     5161 tcaaatagac cccgaacgag atgtaccata tgtattagat acaattaaac ttttaacaga
     5221 agaaaagtta aatgtgccgc taataggatt tactggggca ccatttacat tagcgtcata
     5281 tatgattgaa ggcggaccat cgaaaaatta caattttaca aaagcgatga tgtatagaga
     5341 tgaagcaaca tggtttgctt taatgaatca tttagttgat gtatctgtta aatatgtaac
     5401 agctcaagtc gaagcaggtg ccgaattgat tcaaattttc gattcatggg taggtgcatt
     5461 aaatgtcgag gattacagac gttacattaa accacatatg attcgattaa tcagtgaggt
     5521 taaagaaaaa catgatgtgc cggtaatttt attcggtgta ggtgccagtc atttaatcaa
     5581 tgaatggaat gatttaccga ttgatgtatt aggcctagat tggagaacgt ctattaatca
     5641 ggctcaacaa ttaggcgtta ctaaaacatt acaagggaat ttagatccat caattttatt
     5701 agcaccatgg aatgtcattg aagagagatt gaaaccaata ttagatcaag gtatggagaa
     5761 cggtaaacac atctttaatt taggacacgg tgtattccca gaagtgcaac cagagacgtt
     5821 acgtaaagta agcgaattcg tacacacata tacacaaaga taatcatagt tttacattga
     5881 agcacatgaa agataattca tttaaaggaa ggtattatca atgactaaaa aaatgggatt
     5941 attagttatg gcttatggca caccttataa agaaagtgac atagagccat attatacaga
     6001 tattagacat ggtaaacgtc catctgaaga agaacttcaa gatttgaaag atagatatga
     6061 atttataggt ggtttatcac cattagcagg tacaacagat gaccaggctg atgcgctagt
     6121 ttcagcatta aataaagcat atgcagatgt tgaatttaaa ctatacttag gattaaaaca
     6181 catttcacca tttatcgaag atgcggttga acaaatgcac aatgatggca ttactgaagc
     6241 aatcacggta gtactagcac cacattattc ttcattttca gtaggatcat atgacaaacg
     6301 tgctgatgaa gaagctgcaa aatatggtat tcaacttaca catgtgaaac attattatga
     6361 acaacctaaa tttattgaat attggacgaa taaagtcaac gaaacattag ctcaaatacc
     6421 ggaagaggaa cataaagaca cggtattagt tgtttcggca catagtttgc caaaaggttt
     6481 aatcgaaaag aataatgatc catatccaca agaactagaa catactgcgc ttttaattaa
     6541 agaacaatct aatattgaac atatcgcgat tggttggcaa tctgaaggta atacaggtac
     6601 accttggtta gggccagatg tacaagattt aacacgtgat ttatatgaaa aacatcagta
     6661 taaaaacttt atatatacgc cagtaggttt tgtatgtgag catttagagg tgctttatga
     6721 caatgattat gaatgtaaag tagtttgcga tgatattggt gcgaattatt atcgtccaaa
     6781 aatgccgaat acacatccat tatttatcgg tgcaattgtt gatgaaatca agtctatatt
     6841 ttaatgacga aagaagcgtg aaacgttgtg actaaatcag tggctattat aggagcgggg
     6901 ataacaggtt tatcaagtgc atatttttta aaacagcaag atcctaatat tgatgtaacc
     6961 atctttgaag catcgaatcg tccgggggga aagattcaat cgtatcgtaa agatggttat
     7021 atgattgaac tagggcctga atcttattta ggtagaaaaa cgattatgac agaattagcg
     7081 aaagatattg gattagaaca agatattgtt acaaatacga ctggacaatc atatattttt
     7141 gcgaaaaata aattatatcc gattccaggt ggttcaatta tgggtattcc aacagatatt
     7201 aaaccatttg ttactacaaa attaatatcg ccacttggta aattaagagc agggtttgat
     7261 ttattaaaaa aacctactca aatgcaggat ggtgacattt ctgttggtgc atttttcaga
     7321 gcaagattag gtaatgaggt acttgagaat ttaatagagc ctttaatggg tggtatttat
     7381 ggtaccgata ttgataaatt aagtttgatg agtacgtttc ctaattttaa agaaaaagaa
     7441 gaggcattcg gaagtctgat aaaaggtatg aaggatgaga aaaataagcg tctgaaacaa
     7501 agacaattat atcctggcgc accgaaagga caattcaaac aatttaagca tggtttaagc
     7561 tcatttattg aagcattaga acaagatgtt aaaaataaag gtgtgacaat acgctacaat
     7621 acgtcagtgg atgatataat tacatctcaa aaacaatata aaattgttta caatgatcaa
     7681 ctagaagaag tgtatgatgg cgtattagta acgacaccac atcaagtgtt tttaaattgg
     7741 ttcggacaag atccagcatt tgattacttt aaaacgatgg atagtacgac tgttgcaact
     7801 gttgtattgg catttgatga aaaagacatt gaaaataccc atgatggtac tggcttcgta
     7861 attgcgagaa cgagtgatac agacattacc gcatgtactt ggacatcgaa aaaatggcca
     7921 tttactacac cggaaggtaa ggttttgatt cgtgcgtatg taggtaaacc aggtgatact
     7981 gtggttgatg atcatacaga taatgaatta gtatcgattg tacgtagaga tttaagtcaa
     8041 atgatgacat ttaaaggtga tcctgaattt acaattgtca atcgtttgcc gaaaagtatg
     8101 ccacagtacc atgtcggtca tattcaacaa attagacaga ttcaagcaca tattaaacaa
     8161 acatatccac gacttagagt aactggtgca tcttttgaag cggttggact acctgattgt
     8221 attacgcaag gtaaagttgc tgctgaagaa gtaatcgcag agttgtaagt taattgttgg
     8281 atggtgttgg tgtaggtgcg acgcctttag ataatgtttg atgaaatata gtcattttta
     8341 agttatataa tggtggaagt aatgtgtaaa tatagatact ttcagcggga attatttttg
     8401 tgaaattgaa gcagagacaa aggtttagct gtttttgaat gatattgaaa tgatgacaat
     8461 acactttaat tagaaatgtc actttgcgat ataacatttc taattaaggt gtttttattt
     8521 tacaattata ggtaatcagt cattttttta aaaatagaaa agaagtagta agttgaaagg
     8581 agagaatgag atgacagatg taattattgt acattctatg catggaaata gtagaaatca
     8641 ttggtatcag tggttggagc ataacttaac tttagaagga tatgacgtaa cattatttaa
     8701 ttttgaatcc cctgaagcga agacagtgga tcagtggatt gaagctatga cgaaacaaat
     8761 taatgtacgc aaaaaggata catattttgt aacacatggt ttaggttcaa tcacagcttt
     8821 aaaatatatt gaaatgattg atcagccgat tgaaggcttt ttcagtatag cgggctttaa
     8881 agaagatgca gaaaatatag atttagatat tgatttaagt aatgtaacca ttgattacga
     8941 taatattaaa aagaaagttg acaattttta cggattgagt tctaaaaatg acaaatacgt
     9001 ttcatataaa gagacccagc gtttaatgaa tgcattagag gggaacttgc gaatcgtgga
     9061 agatggaggg cacttcttag aagaggatgg cttcgaaaca tttacagcgt tgcaagatcg
     9121 gatgcaagat tacatgacac gatagttagt gtatgggttg tgttatgttg tgtcagtagg
     9181 tagctgctga ttttgaaaaa ttgttgtaat tgaggcgtca agcaatgtct tagggctaca
     9241 atgttatatt ggtagtcgct gactgaaaga atatgcggtg taacaagctt ttataatttc
     9301 tttttcagaa ggtataggcg ttgtaaactt tataactcct ataccttttt aaatgcttta
     9361 acactattta tgacaactat tagacactta tatgataaaa gggaataaga agcgtttttt
     9421 tacacaaaaa actttgaaaa tgtcgttgac gtacactaca aaaatgaata tactaatatt
     9481 tgtgttcaac agagaacatg ttgctacgat atttaagtaa ttatttaaga gataacattt
     9541 aaattaaatt caaaaagttc ttgactttag gtttttgatt atggtatatt aattaggtaa
     9601 ttcaaaataa tattagcgcg ggat
//
LOCUS       DALAAR010000091         9590 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000091 DALAAR010000000
VERSION     DALAAR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.093"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(140..553)
                     /locus_tag="O2458_002356"
     CDS             complement(140..553)
                     /locus_tag="O2458_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888202.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(582..692)
                     /locus_tag="O2458_002357"
     CDS             complement(582..692)
                     /locus_tag="O2458_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888203.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(778..1509)
                     /locus_tag="O2458_002358"
     CDS             complement(778..1509)
                     /locus_tag="O2458_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ3888204.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1511..2824)
                     /gene="murT"
                     /locus_tag="O2458_002359"
     CDS             complement(1511..2824)
                     /gene="murT"
                     /locus_tag="O2458_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCZ3888205.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3116..3616
                     /gene="ftnA"
                     /locus_tag="O2458_002360"
     CDS             3116..3616
                     /gene="ftnA"
                     /locus_tag="O2458_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCZ3888206.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3698..3790)
                     /locus_tag="O2458_002361"
     CDS             complement(3698..3790)
                     /locus_tag="O2458_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888207.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4005..4559
                     /locus_tag="O2458_002362"
     CDS             4005..4559
                     /locus_tag="O2458_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCZ3888208.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="O2458_002363"
     CDS             complement(4627..5697)
                     /gene="dinB"
                     /locus_tag="O2458_002363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCZ3888209.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5944..6474)
                     /locus_tag="O2458_002364"
     CDS             complement(5944..6474)
                     /locus_tag="O2458_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ3888210.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="O2458_002365"
     CDS             complement(6644..8005)
                     /gene="rlmD"
                     /locus_tag="O2458_002365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCZ3888211.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(8086..9033)
                     /locus_tag="O2458_002366"
     CDS             complement(8086..9033)
                     /locus_tag="O2458_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ3888212.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         3074 a         1749 c         1311 g         3456 t
ORIGIN      
        1 gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa atattcagac
       61 tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt gattgtaatt
      121 acatatcaga tttaaatcat taatgtaaag aggctaattc attaataaat ttagtaaaat
      181 ctgatttagc aacatttatc cttaatgaaa taccagattc tgttgccatg tttgaatgcc
      241 ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa tcaataattg
      301 taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg caatccatat
      361 gtttcaaatt gtccatgtca ctatcaaaga gtgcaaattc aaatttaact tcttgatctt
      421 cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata actacatctt
      481 ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctttt aatatcactt
      541 tagtaggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc ataatcacca
      601 ttactattga taattctagg atagatactc attgtataat aattattcac tagaacaaaa
      661 aaacacacat caatttggtt gtttccatcc aaaataatgt gtgtgtttat acatatatga
      721 ttaattacta aacttcatcg tttatcaata ttaaatccat tgatgcgatg ttcagagtta
      781 acgagatttc ttctgtctat ttgctctgtc tattaatact tgtttcgctt gtatttccgc
      841 ttcattatct atttctttag gctcaaacgg aataccctta cgttcacaag ctttttctaa
      901 cagataatca gtgatttcgt aatttttagg taaaattggt ccatgtaaat aagtacctaa
      961 taaattttta taatgaatgc cttctttttt atcttcatca ttattaccat aaccaaaagt
     1021 aacatgacca agtgtaccga aatcatgata tgttctacca ccgtgatttt caaaacctac
     1081 aatagttcca aaagtatcac tttcgataac aatatctcct gttaatcggt ttgtctttga
     1141 ttcagtataa aaatctaaaa tacctaaccc ttctaattct gtaccatcag gcgtgatata
     1201 ttttttccct aaaaattgat agcctccaca aatcgttaat cccggcatac catcttcaat
     1261 cgcttcttta agtggtgtct taattttact taattctttt gttgctaatg cttgttctct
     1321 atcacttcca ccaccgataa agaaaatatc acattcatca aaggtaatac cttctgtttc
     1381 attgatttct acgacattaa ctttaatatt tcgtttttta gcacgttgtc ttaaagcaat
     1441 aatatttcct atatcactgt ataaattcaa tttatctgac ataaaatgat aaatagtcaa
     1501 ttcatgcata ttatgattga cctccttcaa acgaacggtt taattgttca agcataggcg
     1561 ctaatgatgt atagtttggt attgcaactg tgaaaccttt ataatccata gtctttgccg
     1621 ttgctttata aatatcacgc tcaactataa ttggtacttc aacctctgct aacttcaatc
     1681 gcaattgaag ttcttctgct cgtgtacctg tcacgatgat agcttcaatt tgttgcttag
     1741 ataatttttc aaaatctgca tcataaatcc atgaagtatc tcgaccatct gcagcgttat
     1801 catttagcga aataacatac actttttcgc cttctaattg ttcaccaact gataaacttg
     1861 cattcattcc tgcaggattt ttagctaaat tgatcatcgc ttcttttcgt tcttttttaa
     1921 agtactgcat acgaccattg tctgatgtat acgtttcaaa gccattttta attgtttgtt
     1981 catttaaccc tagctctctt aaaacagtat atgctgctaa cgcgttataa gcgttaaagt
     2041 cacctgcaat tttcatatca tatttttcat cattgatatt taaatataaa aacggtgcca
     2101 catcaaaact tgatatttca tattttgctt gctctcgttt gaaaccacac tgacagtgat
     2161 aatgaccaat ttgattataa tgaatataat cgtattgcaa taagcgacca cagtttggac
     2221 aatatctact ttcattcatc gtactttgtt caaattcatg ggcatgtgct ttcataccat
     2281 agtacacaat cgtatcactt gcgattttca aacgactcac aaatggatca tcagcattta
     2341 gcaataattt gatgccttta ttactaattg tctctgcaat gttattaacc ataatatcaa
     2401 tttcaccgaa gcgatccatt tgatctctaa agaaattagt aaataccatc attgaaggtg
     2461 taacttcttt taacacacgt ggaatcgaac cttcatcaat ttcgattacc gcaattttag
     2521 tcttaggtgt tgattgcatg atgaatgcag aagttatacc tgcagccata ttagcacctt
     2581 cattattgtg tataatttga atattatttg cttttaaagt atgtccaatt aagtttgaag
     2641 tcgttgtttt accatttgtt ccactgataa atacaatatc atcaacttgc tctgctaatt
     2701 ttcttaatat atctgtatcc acttttctag cgatttgtcc aggtaaatct gttcctcttt
     2761 tacctactgc tctacttgct ttacgcgcca atttcgctag atggattgcc gtccactgtc
     2821 tcatgtgttt cctcctcaaa tttccactcg catcattata acatgacaag gcaacttcaa
     2881 aaaagtttct caatcacaaa ttgatacagt gtttctaaat tataatcatt ctcatttaga
     2941 taattaaaaa tgatatttat tctcaattaa atatcgctaa atgatacatt ttctcaatta
     3001 gagtgctaaa ataaggcatt aaattataat tattattatt tacaattgag aatgagtttg
     3061 ttatactaag tttaattcat agtaatttta atttacaaat aagaggtgta tcaaaatgtt
     3121 aagtaaaaat ttattagaag cattaaatga tcaaatgaac catgagtact ttgcagcaca
     3181 cgcatatatg gcaatggcag catactgtga taaagaatcg tacgaaggat ttgcaaactt
     3241 cttcattcaa caagctaaag aagaacgttt ccatggacaa aagatttata actatattaa
     3301 cgacagaggt gcacatgcag aattcagagc agtttcagca ccaaaaattg acttttcaag
     3361 catactagaa actttcaaag acagcttatc tcaagaacaa gaagtaacaa gacgtttcta
     3421 taacttatct gaaatcgctc gtcaagataa agattatgca actatctcat tcttaaactg
     3481 gttcttagat gaacaagtcg aagaagaatc aatgtttgaa actcacatca attatttaac
     3541 tcgtatcggc gatgacagca atgcattata tctttacgaa aaagaacttg gcgctcgtac
     3601 attcgacgaa gaataattaa acatcactac aatagacaga taaatatcat acgacatgat
     3661 aggcatttgg gtcacttaca ataacccaat gtctatatta ttttgcttta cggagatcac
     3721 tagattcatt ttctgaatca ttgatctgcg ttttttcatt ttcaaggcta attattgtat
     3781 ttttagtcat ttatttttta aactactaat gttaataact ctaaatttga tgttgaatta
     3841 atttgacgat tttaaagcat atcatcattt actttttaat cagagttaca tccaaatgat
     3901 agatttcacg ttataccttc acgtataata ttatgtatcg tttgtaagca aatgactaaa
     3961 agtctattaa tatatacatt taattaattg aaaggattga ctacatgata caagatgcgt
     4021 ttgttgcact tgattttgaa acagcaaatg gtaaacgtac aagtatttgt tctgtcggaa
     4081 tggttaaagt cattgatagt caaataacag aaacatttca tactcttgtg aatccgcaag
     4141 actatttttc acaacaaaat attaaaattc atggcataca accagaagat gttgaaaatg
     4201 cacctacgtt tgactacgta tttccatata tgatgcaatt tattgcagat ttacctgttg
     4261 tcgcacataa cgcggcattt gatatgaacg tcttacatca aagcattcaa aatattggtt
     4321 taccaactcc aaatttaact tacttttgta gttatcaact tgctaaaaga accgttgatt
     4381 cgtatcgata cggtttaaaa catatgatgg agttttatca attagatttt catggtcatc
     4441 atgatgcatt gaatgatgcc aaagcatgcg caatgattac ttttaggcta ctgaaaaatt
     4501 atgaaaattt aacatatgta actaatattt atggtaaaaa tctaaaagat aaaggctagg
     4561 actaaataaa atactccctt caaaagtaag cattgtaaaa atgtaaactt tgcagggagc
     4621 tttattttat ataaagtcat atatcgtcat atttttataa gttgattgtt ctaaattacc
     4681 tacagtgaca ccaataagtc gaattggtac atcagggtct tttaaatcgt tataaagtaa
     4741 atatgcaata ttataaatat cttcttcaga actaaccgaa tctcttaaac tcatctgttt
     4801 agatagcgtt tcaaattgat aagttttaat tttaaccgtt acagttttag ctgacttctg
     4861 taatttattt agacgttcag ctgttttacc tgacaattcc catacttttc ttaaaatctc
     4921 ttcatcatca ttcacgtctg ttgcaaatgt gcgttcagtc cctactgatt ttcttactct
     4981 tgatgatttc acttcactat ggtcaatacc gcgtgccttg ttatataaac cccgacctct
     5041 ttttccaaac aaacgtatta attcaaattc cgttttctca tataaatctc taccgttaaa
     5101 aataccatta tcatgcatta cttttttgga agctttacct acgcctggaa aatctccaat
     5161 atccaatgtc atcaaaatat catggacatt ttgataatca atcacagtca taccatcagg
     5221 tttattcata ccactcgcta atttagctaa aaatttgtta taagaaacac ctgcagatgc
     5281 tgttaaatgt gtctgctcta gaatatcttt tctaatatac tgagcaattt tcgaagcagg
     5341 aaggtctggt ctcactaatt ctgtaatatc taaatacgct tcatccaatg acatcggttc
     5401 taccttatct gtataacttc ggaaaataga cataatctgc gcagatgttt ctcggtaagc
     5461 accaaaatta cttgtgacaa agtatccatt tggacataat ttatgcgctt gtgacatagg
     5521 cattgctgaa tggacgccgt attttcgtgc ttcataggat gccgtagaga caacacccct
     5581 actgcttgct ttaccaccaa caatgactgg tttccctttc aatttggggt tatctctcat
     5641 ttcgacttgt gcaaaaaaat agtccatatc tatatgaata attcgtctct cagtcaagtg
     5701 ctcacctccc tactaatttt tacttttata acgcacaaaa atatctcaac ataattatac
     5761 gctgtgtacg atttttttac ataaatcttg cacttagcga taactatatt gagataacta
     5821 caagttgtta taaaatcaat tgctatttaa gcatgatgat gaagacgatt gagtaagaaa
     5881 acataggtaa tctgaaataa ttcaagcaaa ttcattttgt tggtatcatc atattaaaat
     5941 ttattattga gtcggctttt gatgatacaa ataaatacta tcttcatctt gttgaacata
     6001 tttaaaacta ctagatattg ttaatgcaac aagcagaata tctataaagc aataagcggt
     6061 atgcatacta aacataaaaa taagtgatga ataaccaaat accttaatta aaataagcaa
     6121 gccagtactt aataggatta gtggtgacag cataataatt gagaattgcc atttgttgaa
     6181 gcaagcatct gctgtttgga ataagattct gtcttttttt atattaaaca taggtttgct
     6241 atctttttta aataaaagaa ataatgctct atggataagt tcatgtaaaa tcaataaaat
     6301 aatgaatcca gcaaacccat atacaagatt gatgatgata ttttgatcga caaccgctgt
     6361 gacacctaac gcccacttat acgtaaataa aatcacgaat aacgcaataa caagttgcaa
     6421 gataataaac cttcgcattt gaaaattatt tgtcgttaaa tcaattttat gcattaccaa
     6481 ccctcccgat catgacattc ttattcttct ttaaatatag tatacaatgt cacatttaat
     6541 ttaaaaagtt catatcaaga aagtaaattg gctgtaataa aattttaata tacgacttct
     6601 ttcttcactt attaaggcga aattttatct caaatcatgt gcgctatttc aaattgaata
     6661 atgccactgt ctcaacatgt gttgtttgtg gaaacatatc taccggtgtt acctcttcaa
     6721 gttgatattt ttcagctaat aataatgcat cacgttgctg tgttgcggga ttacatgaaa
     6781 tatagacaat acgcttaggt tctaatgtaa gcaaagtctg aataaacgtt tcgtcacagc
     6841 cctttcttgg cggatcaacc attacaacat ctggtttaat cccttgtgct ttccattgta
     6901 aaataacttc ttcagctttc ccacagacaa aagttgtatt attgcattgg tttatagtcg
     6961 cattttgttg tgcgtcttca attgcagaag gtactacttc aacaccgtat acatgttttg
     7021 caagtggtgc catatatagc cctattgttc caataccaca ataggtatct aatacaactt
     7081 cattacctgt caattgcgca tactcaattg ctttattata taatttctct gtttgttcag
     7141 aattaatttg gtagaatgac tgatcactta ttttaaatgt actatctgtt aattgatcaa
     7201 taattgtatc tttaccatat agcgttatag attgacgtcc cataataaca ttagagtggc
     7261 tatcattaat gttttgttta atgcttgtca cattaggaaa tgcatctaat atcttctcaa
     7321 caacagcatt tttttgtggc cactttttac cattagttac aaaaataatc atcatttcgt
     7381 ctgtatgata tcctgttctt acaaccaaat gtctcattaa accttttttc aattgttctt
     7441 gataaatact tacatttaaa tcttttaaaa tagatttaac ttcattcatc acttcttgat
     7501 gttgtgaatc ttgtattaaa caactttcca tgtcaataat gtcatggctt ctttgacgat
     7561 aaaagcccat aataacttca ttctgttcat tcttaccaac tggaatctgg gacttgtttc
     7621 gatatctcca aggatctgtc atgccaactg tatcgttaat cttagaatta tcaaaatgcg
     7681 cttttcgctg aaacaaatta atcacttgtt cctttttcat ttcaagttgt gcttcgtatg
     7741 ataagtgttg aagttggcac ccaccacaac gttcataata tatacatggc ggtgcaacac
     7801 gattctcact tctcgtattt atattaagca acttacctat cgcaaagttc ttttttactt
     7861 tgataatttt atattcaatt tgttcattaa ttaaagcttg tggtatgaaa ataggaaagc
     7921 gatctatttt tacgacacca tggccttcat gcgttaaatc aacaactgtt cccgttttta
     7981 tgtcattttt agctattgct tgcaaaattt tacctccaaa atgaacaggt taggaacaaa
     8041 attatgcgct tcctaacctg ccattatata tttcactatt tctgtttatt cttcgattaa
     8101 attgtcatca acatgatcat tatttattaa ctcttcattt acaatatcat taggtgcaaa
     8161 gacatctata tgacgttcta gatttaagaa attcgctggt aatttaccac catattctcc
     8221 atctacattt agttgtaagt ctgtgaatga tgaaatatta attgcctttg ctttttcata
     8281 aataacttta ggatgcttag tatgttctcc tcttgaagct aaagtcataa tatgaccaag
     8341 ttctgcaagg tttgattttt caactataat taacgtaaaa tagccgtcat ctaacttagc
     8401 gtccggcact aatttttcaa atcctgccat tgaatttgtt aaacctaaaa agaataataa
     8461 tgcttctcct tggaaaacat taccatcata ttcaattctt aaatctacag ctttcatttg
     8521 aggtaacatt tcgaaacctt tgatgtaata agcaaatgga ccaacaatag atttcaattt
     8581 actcggtgtt tcataagaga cttgcgtcaa ttgtccgcct gcagctaaat taataaagta
     8641 tcgattattc attttaccaa tatctacttt agtagaatga ccttcaatga tgacatcaag
     8701 tgcccccatg atgtcattag gtatatgcaa tgcacgtcca aagtcattaa cagtacccat
     8761 aggaatgaca cctagcttag gacgattagg cttttctgcg ataccattaa ctacttcatt
     8821 taatgttcca tcaccacctg cagcgattaa tacatcataa ttttcatgca tagctctttc
     8881 tgcttcaagt gtggcatcac ctattttctc ggttgcatat gcactcgttt catatcccgc
     8941 tttttctaat tttattaagg catcaggtaa ttctctttta aatagctctt tacctgatgt
     9001 cgggttataa atgattctag cacgtttcct catatcttat ccctctactt aaaattcata
     9061 tattttaact tcatctttgt ttcgtctaat agggagtggg acagaaataa tatttaacaa
     9121 aatttatttc gttctacccc aacttgcatt gtctgtagaa tttcctttcg aaattctcta
     9181 tgttggggcc ccaccccaac ttgcacatta ttgtaagctg acagaaagtc agcttctttg
     9241 tttgggggcc ccgccaactt gcacattatt gtaagctgac agaaagtcag cttctatgtt
     9301 ggggccccac tagaattgaa aaaagcttgt tacaagcgta ttttctttca gtcaactaca
     9361 gccaatataa cattgtagtg cctaggacat tgaatttatg acccaggctc agtcttattt
     9421 catcattctt aatatcgtta aagaccaact tgtatcttaa acaaatacta tctcaatatg
     9481 tacaaagctt gttatttatt cagcattttt tgccgttctt cattatatag acttcgtcag
     9541 ttatgctatt ttacctttaa aatgatgttg taaatataat gttgtcttta
//
LOCUS       DALAAR010000092         9484 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000092 DALAAR010000000
VERSION     DALAAR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.094"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(574..2574)
                     /locus_tag="O2458_002367"
     CDS             complement(574..2574)
                     /locus_tag="O2458_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888213.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(2571..3326)
                     /locus_tag="O2458_002368"
     CDS             complement(2571..3326)
                     /locus_tag="O2458_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888214.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(3434..4321)
                     /gene="nsaS"
                     /locus_tag="O2458_002369"
     CDS             complement(3434..4321)
                     /gene="nsaS"
                     /locus_tag="O2458_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCZ3888215.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(4332..4997)
                     /gene="nsaR"
                     /locus_tag="O2458_002370"
     CDS             complement(4332..4997)
                     /gene="nsaR"
                     /locus_tag="O2458_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCZ3888216.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(5023..5223)
                     /locus_tag="O2458_002371"
     CDS             complement(5023..5223)
                     /locus_tag="O2458_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888217.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            5421..6845
                     /locus_tag="O2458_002372"
     CDS             5421..6845
                     /locus_tag="O2458_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCZ3888218.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6915..7025
                     /locus_tag="O2458_002373"
     CDS             6915..7025
                     /locus_tag="O2458_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888219.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(7128..7583)
                     /locus_tag="O2458_002374"
     CDS             complement(7128..7583)
                     /locus_tag="O2458_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ3888220.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(7828..8589)
                     /locus_tag="O2458_002375"
     CDS             complement(7828..8589)
                     /locus_tag="O2458_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ3888221.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(8705..>9484)
                     /locus_tag="O2458_002376"
     CDS             complement(8705..>9484)
                     /locus_tag="O2458_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ3888222.1"
                     /translation="DSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTD
                     ALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT         3092 a         1641 c         1436 g         3315 t
ORIGIN      
        1 aaaatatatg aaacaaattt gactcatctt tttcaatatc taaatgacac gatgcatgat
       61 tttttaattt tgtaattaac ttccttttaa attagactga attgtgacaa ttttcaaaca
      121 ttttaatgtg taaatcattg gcaaaatata cgaattgcat ttaaatcgtc aatttacata
      181 tatttttgcg tagtaatcaa tcgttatcat tgttatttat cgttacatta tttcgagtat
      241 cagtatgtat ttcgggcttc gtttgataac gacatttctt tgtgacatcg cttcatcagt
      301 gtaacaacaa atacaatgat ttcgtgatgt tagttaccca ttttatgtgt tgcataaaat
      361 atgttgttat aaaacatttt aaatcatttt atataaacaa tctatatatt tttggcattt
      421 ccaaaatatc acttgttata ttaaaaaccg acaagacatt ttatcttatc ggttgaaatt
      481 tgttattgtt atttgtaatg tttttaggtt tctttttaat ataatatatt tcagtgaaaa
      541 tacatgattg attgtgattt cactgaaaca tggttaattg cgttgttgat gaataacttt
      601 agcataaata taggaagtta ttttgtacat cgccatatat agaaacgaaa ttataatgac
      661 agctagtacg taacttgtta aaaatatatg atggttatta atacctatca tatttagtaa
      721 cgtatataca atgttactag aaattaatgt gtgaatcagt gctactgtta ttggtattgc
      781 gaacaagaaa gtcatttgat ttcgtgttat ctttgctatt cttccattat ctaaaccaag
      841 ttttttcata atgtcataat tgtgctgatt tgcataggct tctgagactt gtttataata
      901 catcattaga aatattccaa tgacaagtac aattgatact acgccaccta caaatattag
      961 tccacctgtc aaacgatccc aaaccattag catttctgat ttagaattta ttgacgcatc
     1021 gtattttttc gatagctttt gtatttgtga tgttgttaat ttcgtctttc caaatacatt
     1081 aaagtttatc gatattgtcg aattcttttg ttcatgtgca taaaatcctt ttactttttg
     1141 taattgattg ttatctttaa caattaaaac catactatct tgaatcatca tcaaatttaa
     1201 cgcatgtgcg tcaagttgtt tcactttaaa gacttcatta ttaagaccga ccttagactg
     1261 ctttttaaaa ataggtacat tcgtaatcat acctatactt tgattgtcta agtgtaattt
     1321 gttttggtga tatcgattat aatcttgtgc agtaaaagct gtaatcatta ctgattgtct
     1381 tgaaaatatc gcgccttcat taccaatgaa gtctattgga atatcatcac tagcctgttt
     1441 taacttgtaa taagcacgat tcttttctaa agaagctcta aataatgtcg tttcatatac
     1501 tttgaagtct tttgcattaa ccgaaccctg tatatcacgt ttaatattct caattgtttg
     1561 ttgacgttct atttgtgact tagaattgtc gctaaatgac aaatcataat cattcgtaat
     1621 caatttcgta atggtatgat tcatatcacg ataggttgtt aatgtcattg tgagcgttac
     1681 aatcaaaaat gtacacagca aagtgattgt tgcaagactg actgcattca ttttaagacg
     1741 tacacgcatc ccaactacca caaaaaaata ccgtggatga tagtaaactt ttgatacctg
     1801 ctgcaatata ctaataatta tttcacttat acctacaaaa aatgcataag taccgataat
     1861 aactaatcct atgacaatcc atattttaaa aaaggcacca aacgtcgtat cttgttgtaa
     1921 tgcaataaag taacctaaat atagtgctgc gcttcctata acaattaaaa cgtaacgcaa
     1981 ccatcgtgat atgacactag aatcggaacg atgttgataa gttatcggcc gttgaaaatt
     2041 aatactaaac aagttgaata taagtagcat gcccattaat acagcaatga taatcaaagt
     2101 aataaacatc gctacagagt caaatggata ataccttaac gtcgcaactt cactgcccat
     2161 tattttctgt ataaacaaga aaaatatcgc accaagtaag tatccgccgg caatactaac
     2221 gactgaaatt atcacaaatt gtaagatagt ttccatcact acaattaaac gcatactttt
     2281 cttggtcatg cccaatgtca taaaaatgct aaactctttt cgtctttgtg acatcataaa
     2341 gtgatttgca tagaaaataa aaataaaagt taaaagcgcc ataaaaaaat tagctatgat
     2401 aataaatggt actaggaagt cattcttctg ttttatgtag ctatttaacc caattgacac
     2461 aagcgtatat tcaatcatga atagtacact taccgctaaa atgaatggaa tgattacaag
     2521 tcgctgcgta ataaattgac gtcgcactat gtgccataac aatcttaact tcatatattg
     2581 acacttcctc cattcacaag tgctaagcta tctgttattc gttgttgaaa agctaattga
     2641 ctttcttcac cacgatatat ttcatgatat agacgcccat ctttaataaa aatgactcgc
     2701 tctgcataag acgcatcgat atttgaatga gttaccatta aaattgtctg ttccaattga
     2761 tgaatctctt gaaataacat catcaatgcc tttgatgttt ttgaatcaag tgcacctgta
     2821 ggttcatcgg ctagtaaaat cgtcggctta gtaactaatg cacgtgcaat ggcaatgcgt
     2881 tgcttctgac ccccagagat ttcagaagga tacttgttta agaatccctc taaatgtaat
     2941 tgtactgcca actgatgtac cctttgttct atatcttttc gtttagcacc agctaaaatt
     3001 aatggcatca ttatattttc tttattcgtc attgttggta aaagattaaa atcttgaaaa
     3061 acaaaaccta ccatttgttg acgatacaat gctttacttt tatttttcat attatttaaa
     3121 tgtgcgccat ccacaataat gtcaccttca gttagtccat caaaagaagc aattaaattt
     3181 agtagtgtag acttccctga cccagactca cccataattg caacaaattc accagctcca
     3241 actgataaat tcatttgatt aagtgctgtc gtagcattca aacctttacc ataaaccttt
     3301 tttacatgtt tcacttctag caacatggca tgcacctcat ttctttgtta gttctatatt
     3361 aaaacaaaaa aatactaata acatttaaat tagtaaaaaa atgttactag tatacttaca
     3421 atattgaaag tttttattca tctggaaatt gaatcgtaaa tgttgtaccc tcattttgtt
     3481 tagatacgac tgaaacagga tggtttgtgt gtgttgaaat ttgttttacg ataaataaac
     3541 caatcccact tgagttactt tggcgctggc cattataacc tgaatagccc ttatcaaata
     3601 ttttaggcaa gtccgcttca ctaataccga taccattatc ttttacgtgt aattgattgg
     3661 attgctcatc aaattcaatc catatatctt tacctctcgc atacttaagt gcattattta
     3721 ttaattgttc tatcattaaa gaggtccatc taacgtcagt taatacttcg tgatgacaag
     3781 gttcataatg gatttttgtt ttttgatcaa taaactgtat tgaatatttc ataataattg
     3841 ggcgaatgat attattaatc gaaattttag tgacagaaat atcagaagtt tcatttaata
     3901 actttaaata actaagtgct aaacttgtat agttatcaat ttgaataacc tcttgacgaa
     3961 cacgattaac aacattaggc tcatctcttt caagtaacag ttgtgctgca gtaatgggtg
     4021 ttttcatttg atgtacccat gttaaaaagt agctctctac atcattttta tattcaattt
     4081 gttcattttt aagctgatac aacgcatttt ctaagttttc aatttgttgt tgttggctta
     4141 tagtttttac aaaacttaaa tatttaatac ctatgaatat gagtaataat aataaaataa
     4201 cgccaagtgc tagtaaatat gcttctaatg gcaaatgata caggtaaaac attaagccaa
     4261 acaaagcaaa aataactatg actattgcta tttcctgagt aatactttta agaaaggtca
     4321 ttgcattcac cctatacttt atatccgaca tttttctttg taatgataaa atcattaacg
     4381 ccaatagtat ttaatttttt acgcaggcgc gtcatgttaa cagctaatgt gttatcatct
     4441 atgaagtttt ctgattccca acatttttca attaaagcag ttctacttac atatttatct
     4501 tcattttgaa ataataactt taatatttgt aattcagtca aagatagctg tatgttttgt
     4561 tcttgataca cgacttttgc ttcatctaat attaatgtac aacctttaac cgtcaatgaa
     4621 tcattagcta ctgacaagtc ataagttcgt ctcaataatg cttgaatttt ggcaatcgtt
     4681 aatgacaagt taaatggctt ttcgataaaa tcatctcccc ccatttgtat tgccataatt
     4741 tggtccatat tatcaatacg ggaactaata aatataattg gcacattaga tgtttttcgg
     4801 atttcttgac accaatgaaa accatttaac gttggcaaat taatatctag caatacaagc
     4861 tgaggttgat tttggttaaa gatagacagt atatcatcaa attgttcaac gacaataaca
     4921 ccgtaattcc attttttaag ttcagatgct aaactctctg ctataacaaa atcatcttca
     4981 acaattaata ttttcatcta taatcttctt ccttcaattg aattatcctt tgttatcttt
     5041 ttgttcttta tcctgctgca atgacggctc ttgatttgaa ctttccgtgt catttgtatt
     5101 ttgatttagt gtatttgctt tttctttctc gagttgcttt ttcttcgtac gtttacgctc
     5161 ttctaaaata gacaacacta taaagaatat gattaataat ggagataaca ttactagtat
     5221 catttttcca cctcaaatta tatttaattc tatattttca cacgcttcta ttctaacgct
     5281 ttctataaca aattgtcata tatattcatt ttttgttatt ctaaatcact ctgtttacat
     5341 atttttaact aaaactacat aattgctcaa aattcctctt ttacactgaa gacatagtca
     5401 tatactcagg aggatgtctt atgttatcag ttaaaaaagc cacaaaattt gcagttgcta
     5461 accttattat cgtgtcaacg gtttttagtt ctgtcacacc tgcaattgcg caatccgata
     5521 aaagttctaa agatgaaagt ttcgcggttg gtaatactaa aaatccaaaa aatgttatct
     5581 ttttagttgg cgatggcatg ggtccatcat ttaacacagc ttatcgttat tataaaaatg
     5641 atccgcatgc taaagaaatg acccctactg cttttgattc atatttaaaa ggcacaaatc
     5701 gtacatattc aaatgaccca aaacagaata ttactgattc tgcagctggc ggaacagcct
     5761 ttagttctgg tcataagaca tataatggtg ccatcggtgt tgacagtaac aagcagaaag
     5821 taaagacggt gcttgaacgt gcaaaagaaa aaggtaagtc gactggtctt gtttcaacag
     5881 ctgaattaac agatgcaaca ccagcagcat atgctgcaca cgtgacttct agagatgata
     5941 aaaacgaaat cgctaaacaa ttttataaag ataaaatcaa cggaaagcat aaagttgatg
     6001 ttttattagg cggcggcgct aaatatttcg gtaaaagtaa tggcaattta gataaaaaat
     6061 tcaaaaaaga tggttacgac cttgccacga acagtaagga gttatcaaaa tctgataaag
     6121 ataaagttct tggtttgttt gcagataaaa atatgccact tgcaatagat gcttctaaag
     6181 acgaaccatc attagctgat atgcaacaaa gtgcacttag taagcttgag cgcaataaaa
     6241 aaggattctt tttaatggtc gaaggtgctt ctatagataa atcagcgcat tccaacgata
     6301 ttacaggcgt tatgtctgaa atggaaggtt ttgaaaaagc attcgatgac gccattcaat
     6361 atgctaagaa acataaagat acacttgtcg ttgcaacagc agatcactct actggtggtc
     6421 taacaattgg taaagataaa ggatacgaat ggaatcctca accgattaaa tctatgaaac
     6481 actctggatc atatatgaca gaaaaaatgg ctaaaggtga agaccctgaa aaagttatta
     6541 atgaaggtta tggatttgaa ttttcacaag atgatatgaa aaaggttaaa aaagagaata
     6601 aaaagctaaa aagactactt aaaaaagaaa aagacgaaaa atccgcagaa gtagaaacgc
     6661 aaacaaaagc cctacaacac gctattcaga agccagtaaa tgataaatct tatacagggt
     6721 ggacttccga tggacataca ggcgaggatg ttaataccta cgcttacgga cctagatcag
     6781 aatcatttag tggtaatatc gaaaatacag aaagcgcaaa aataatattt gatatattca
     6841 agtaaagcat aaaaaaattc ccgagcacta taacaaggtg ttcgggaatt ttttatggtt
     6901 taatttagta ctcaatgtta ggtgcttggt taacacgttc ttggtatttt ttaaatgcga
     6961 agtataaacc gccaactaat gttaaaataa ctgctaattt tttcatcatt caaactcctt
     7021 tctaagatgt gttgaaactg agttcaatta aattatatgt ttttattata cactttttga
     7081 catatttttt aaatttaaga atgcgaagat ttttaacatt tctgatgcta gctttctttt
     7141 tcatctttca aatgcttaat aaaatcaaag ccaggtcgcc ctttagccaa tgcttccatc
     7201 acatcatacc aatccgaacc gacttgttga tatatatttt gcaataattc atcttgcgcc
     7261 ttgttcaatt cagcaatgag ttgatcgcct ttatccgtcg aatataattt tttgacacgt
     7321 cgatcagtct ctaaaacttt ttcaataatg agaccttgct cttttaattt ttgaagtgtt
     7381 gcatgtgatc cttgtttaga aatttctaat atttctagta atgatttaat agtaatacca
     7441 ggtaatttat tgataaaaaa caaaaaacga tgatgctgac gactcatacc atacttctct
     7501 ataatttcat cagcggtagt aataaaggtt ttatatgcaa aataaaacaa catatgctca
     7561 taatcgtttt tattggtcga catgatgatg actcctttca aagcgttaac ctcattataa
     7621 taaaatttat aatgtaagtc atacaaatga tgataaaact ataagctaaa tctaagtttc
     7681 acgagaaaca tttttgtgta tcagcaaatt gcgccaatca tattaataca aaaataattt
     7741 aaacacttag aaatgttagt cgtgcgtaaa tagtttgtat ctctcactct gtgtatttaa
     7801 tgcagccaca tattttccaa gaaataatta atcattcacc atccatgtta tagcacgctt
     7861 aatcgcttga tcccaatatg cataatcatg atctcctggt ccatcttcaa attgataagg
     7921 aacatttatg cgtgataaat aatcgataaa atctaagttg tcttgatata aaaagtcttg
     7981 tttaccacac ataatgagca attttggaat ttgtttatct tcagctacag ctttgtctag
     8041 caagtaatac ggatcatgtt cagttccttt aacacttgaa agattgccaa ttatggcctc
     8101 ttttgaaaaa tcattccact ctagatccat taaattttgc gcttcaaaca cagcagataa
     8161 tggtacagct ttggcaaatt tatccccttg tgttaatgca aatttaattg tgccatatcc
     8221 tcccatagag tgacctgcta taaaattgtc atcacgcttt ttggaaagtg gaaatatttg
     8281 atgaacataa tcatacactt ccaaaatata atcataatag ctatgaccat atgccatgtt
     8341 agcatatgcg ctatgatcca cattgggcat aatcacagct aatttgtgtt cattcgcata
     8401 cctttctatg cttgtatatc tcatatatgt cgtttcatca cttgataatc catgtaacaa
     8461 cattaaagtt tttaatggtt taacagttgt atcgctatta aagaagcttt gatcttccgg
     8521 taaaatgact gtcaaatttt gatgcatacc aattgttggt gaatgatagt ttaatgaaat
     8581 ataagccata cgtcatgacc cctttctaat tctactttat caacatttta cgcttaatca
     8641 attcacttta aaatcatttt caacaaaaaa accgaataca aatgtattcg gcctaaaaaa
     8701 gtatttacgc tttttcttta tgatcttgct tgcgttttct aaacaatagt aatgatccta
     8761 ataatgccat cattgcacca aataaagttg catttgtgtt ttcgctctta tctcctgttt
     8821 ctggtaaagc atcagttttg tgttgttttg ataccttatt agaatggttt acttcacctt
     8881 taggatttga tggtgctttc tgttcattat ttggtggtgt aactcttgaa tcggagtcac
     8941 tatctgagtc tgagtcgcta tctgaatccg agtcgctatc cgagtctgag tcgctatctg
     9001 agtctgaatc gctgtctgag tctgagtcgc tatccgagtc tgagtcgctg tctgaatctg
     9061 aatcactgtc tgaatccgaa tcgctatctg aatctgaatc gctatccgag tctgagtcgc
     9121 tgtctgaatc tgaatcgctg tctgagtccg aatcgctatc tgaatctgag tcgctgtctg
     9181 agtctgaatc gctatctgaa tctgagtcgc tatctgagtc tgagtcgctg tctgagtctg
     9241 agtcgctgtc tgagtctgag tcgctgtctg aatctgagtc gctgtctgaa tctgaatcgc
     9301 tgtctgagtc tgaatcgctg tctgaatctg aatcgctgtc tgagtctgaa tcgctatctg
     9361 agtctgaatc gctatctgag tctgaatcac tgtctgagtc cgagtcactg tctgaatctg
     9421 actcactatc tgattctgag tcgctatctg attctgagtc gctgtctgaa tctgaatcac
     9481 tgtc
//
LOCUS       DALAAR010000093         9410 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000093 DALAAR010000000
VERSION     DALAAR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.095"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            103..999
                     /gene="xerC"
                     /locus_tag="O2458_002377"
     CDS             103..999
                     /gene="xerC"
                     /locus_tag="O2458_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCZ3888223.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            996..1541
                     /gene="hslV"
                     /locus_tag="O2458_002378"
     CDS             996..1541
                     /gene="hslV"
                     /locus_tag="O2458_002378"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCZ3888224.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1607..3010
                     /gene="hslU"
                     /locus_tag="O2458_002379"
     CDS             1607..3010
                     /gene="hslU"
                     /locus_tag="O2458_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCZ3888225.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3035..3808
                     /gene="codY"
                     /locus_tag="O2458_002380"
     CDS             3035..3808
                     /gene="codY"
                     /locus_tag="O2458_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCZ3888226.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3859..3951
                     /locus_tag="O2458_002381"
     CDS             3859..3951
                     /locus_tag="O2458_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888227.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            4150..4917
                     /gene="rpsB"
                     /locus_tag="O2458_002382"
     CDS             4150..4917
                     /gene="rpsB"
                     /locus_tag="O2458_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCZ3888228.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4951..5064
                     /locus_tag="O2458_002383"
     CDS             4951..5064
                     /locus_tag="O2458_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888229.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5099..5980
                     /locus_tag="O2458_002384"
     CDS             5099..5980
                     /locus_tag="O2458_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCZ3888230.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6117..6839
                     /locus_tag="O2458_002385"
     CDS             6117..6839
                     /locus_tag="O2458_002385"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCZ3888231.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6858..7412
                     /gene="frr"
                     /locus_tag="O2458_002386"
     CDS             6858..7412
                     /gene="frr"
                     /locus_tag="O2458_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCZ3888232.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7785..8555
                     /locus_tag="O2458_002387"
     CDS             7785..8555
                     /locus_tag="O2458_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCZ3888233.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8562..9344
                     /locus_tag="O2458_002388"
     CDS             8562..9344
                     /locus_tag="O2458_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCZ3888234.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         3495 a         1294 c         1849 g         2772 t
ORIGIN      
        1 gttacaatta tttaattctc aaaagattta gaattgatta tcaaattact gtaagccctt
       61 tgctgtatat gctacaattc ttattgatgg agggtaaatg tattgaatca tattcaagat
      121 gcgtttttaa atacattgaa agttgaacgg aatttttcgg aacacacatt gaaatcatat
      181 caagatgact taattcagtt taatcaattt ttagaacaag aacatttaga gttgaatact
      241 tttgaataca gagatgctag aaattatttg agctatttat attcaaatca tttgaaaaga
      301 acatctgttt ctcgtaaaat ctcaacgtta agaactttct atgaatattg gatgacgctt
      361 gatgagaaca ttattaatcc atttgttcaa ttagtacatc cgaaaaaaga aaaatatctt
      421 ccgcaattct tttacgaaga agaaatggaa gcgttattca aaactgtaga agaggacact
      481 tcaaaaaatt tacgggatcg agttattctt gaattgttgt atgctacagg catccgtgtt
      541 tcggaattag taaatattaa aaaacaagat atagattttt acgcgaatgg tgttaccgta
      601 ttaggaaaag ggagcaaaga gcgctttgta ccgtttggtg cttattgtag acaaagcatc
      661 gaaaattatt tagaacattt caaaccaatt cagtcatgca atcatgattt tcttattgta
      721 aatatgaagg gtgaagcaat cactgaacgc ggtgtacgat atgttttaaa tgatattgtt
      781 aaacgaacag caggcgtaag tgagattcat ccccacaagc tcagacatac atttgcaacg
      841 catttattga atcaaggtgc agacctaaga acagtacaat cgttattagg tcatgttaat
      901 ttgtcaacaa ctggtaaata tacacacgta tctaaccaac aattaagaaa agtgtatcta
      961 aatgcacatc ctcgagcgaa aaaggagaat gaaacatgag taatacaaca ttacatgcaa
     1021 caacaattta tgctgtaaga cataatggga aagcagctat ggctggagat gggcaagtaa
     1081 cgcttggtca acaagtcatc atgaaacaaa cggcaagaaa agtgcgacgt ttatatgaag
     1141 gtaaagtgtt agctggtttc gcaggtagtg tagcagatgc gtttacgtta tttgaaaaat
     1201 tcgaaacaaa attacaacag tttagtggta acttagaaag agctgctgtt gaattggcac
     1261 aagaatggcg aggcgataaa caattacgtc aattagaagc tatgctaatt gtaatggata
     1321 aagatgctat tttagttgtc agtggaactg gcgaagttat tgctccagat gatgacctta
     1381 tcgctattgg atcaggaggc aactacgcat taagcgcagg acgtgcattg aaacgccatg
     1441 catcgcattt gtctgctgaa gaaatggcat atgagagctt gaaagtagcg gctgatattt
     1501 gtgtctttac caacgataat attgttgtcg aaacactata ataatcagag cacgataaat
     1561 aattacgagc aattaatttt agttaaaaga cggaggaatg aaattaatgg atacagctgg
     1621 aataagatta actccaaaag aaatcgtatc taaattaaat gaatacatcg ttggacaaaa
     1681 tgatgctaaa cgtaaagtgg caattgccct acgtaatcga tacagaagaa gtttattaga
     1741 tgaggaatca aagcaagaaa tttcacctaa aaatattttg atgattggac caaccggcgt
     1801 tggtaaaact gaaattgcaa gaagaatggc caaagttgtc ggcgcgccat ttataaaagt
     1861 agaagctact aaatttactg aggtaggtta tgtaggacga gatgttgaaa gtatggttag
     1921 agatcttgtt gatgtttcag taagattagt caaggcgcag aaaaaatcat tggtacaaga
     1981 tgaagcaaca gctaaggcca atgaaaaact tgttaagtta ttagttccaa gtatgaaaaa
     2041 gaaagcgtct caaacgaata atcctttaga gtcacttttc ggaggtgcaa ttccaaattt
     2101 cggacaaaat aacgaagatg aagaagaacc acctactgag gaaattaaaa caaaacgttc
     2161 tgaaattaag agacagctag aagaaggcaa acttgaaaaa gaaaaggtaa gaattaaagt
     2221 cgaacaagat cctggtgctt taggtatgct aggtacaaat caaaatcagc aaatgcaaga
     2281 gatgatgaat caattaatgc ctaaaaagaa agttgagcga gaagttgctg ttgagacggc
     2341 aaggaaaatc ttagctgata gttatgcgga tgaactaatt gatcaagaaa gcgctaacca
     2401 agaagcgctt gaattagcag aacaaatggg tatcatcttt atagatgaaa tcgacaaagt
     2461 tgcgacgaat aatcataata gtggtcaaga tgtctcaaga caaggtgttc aaagagatat
     2521 tttacctata cttgaaggta gcgttattca aaccaaatat ggtactgtga atactgaaca
     2581 tatgctgttt ataggtgctg gagctttcca tgtatctaag ccgagtgact tgataccaga
     2641 attgcaaggt cgttttccga ttagagttga acttgatagt ttatcggtag aagattttgt
     2701 aagaattttg acagaaccaa aattgtcatt aattaaacaa tatgaagcat tgcttcaaac
     2761 agaagaagtt actgtaaact ttaccgatga agcaattact cgcttagctg agattgctta
     2821 tcaagtaaat caagatacag acaacattgg tgcacgtcga cttcatacaa ttttagaaaa
     2881 gatgctagaa gatttatcat tcgaagcacc aagtatgccg aatgcagttg tagatattac
     2941 cccacaatat gttgatgata aattaaaatc aatttcaaca aataaagatt taagtgcatt
     3001 tattctataa aaatatacaa aaggagaaaa attcatgagc ttattatcta aaacgagaga
     3061 gttaaacacg ttacttcaaa aacacaaagg tattgcggtt gattttaaag atgtagcaca
     3121 aacgattagt agcgtaactg taacaaatgt atttattgta tcgcgtcgag gtaaaatttt
     3181 aggatcgagt ctaaatgaat tattaaaaag tcaaagaatt attcaaatgt tggaagaaag
     3241 acatattcca agtgaatata cagaacgatt aatggaagtt aaacaaacag aatcaaatat
     3301 tgatatcgac aatgtattaa cagtattccc acctgaaaac agagaattat tcatagatag
     3361 tcgtacaact atcttcccaa ttttaggtgg aggggaaaga ttaggtacat tagtacttgg
     3421 tcgagtacat gatgatttta atgaaaatga tttggtacta ggtgaatatg ctgctacagt
     3481 tattggtatg gaaatcttac gtgagaagca tagtgaagta gaaaaagaag cgcgcgataa
     3541 agctgctatt acaatggcaa ttaattcatt atcttattct gaaaaagaag cgattgaaca
     3601 tatctttgaa gaacttggcg gtacggaagg cctattaatc gcatcaaaag ttgcagatag
     3661 agttggtatt actagatctg taattgtaaa tgcactacgt aaattagaaa gtgctggtgt
     3721 aattgaatca cgttctttag gaatgaaagg tactttcatt aaagttaaaa aagaaaaatt
     3781 cttagatgaa ttagaaaaaa gtaaataagt cgatgagtct gggacataat tcaatgtcct
     3841 agtcgtgacg atgttatctt ggcggtagtc aatgaaatga ctttgatgat tatgttgaag
     3901 tcatttcttt atttttgtct aattttcaat caaattgata tttcacatta acttgataga
     3961 attaccttta aatttatgga aaaatgcttg aaaaacattt gctttagaca cggcattatg
     4021 ttatatttat tttcggctat aaagccaata cacacatatc aaacgtgatt gtgtaaaggg
     4081 tgcaatattt atatattgtc tttacaatag tttgctatgg aggtaattaa ccaataggag
     4141 gaatttataa tggcagtaat ttcaatgaaa caattactag aagcgggtgt tcacttcggt
     4201 caccaaacac gtcgttggaa cccaaaaatg aaaaaatata tcttcactga gagaaatggt
     4261 atttatatca tcgacttaca aaaaacagtg aaaaaagtag acgaggcata caacttcttg
     4321 aaacaagttt cagaagatgg tggacaagtc ttattcgtag gaactaaaaa acaagcacaa
     4381 gaatcagtta aatctgaagc agaacgtgct ggtcaattct acattaacca aagatggtta
     4441 ggtggattat taactaacta taaaacgatc tcaaaacgaa tcaaacgtat ttctgaaatt
     4501 gaaaaaatgg aagaagatgg tttattcgaa gtattaccta aaaaagaagt agtagaactt
     4561 aaaaaagaat acgaccgttt aatcaaattc ttaggcggaa ttcgtgatat gaaatcaatg
     4621 cctcaagcat tattcgtagt tgacccacgt aaagagcgta atgcaattgc tgaagctcgt
     4681 aaattaaata ttcctatcgt aggtatcgtt gacactaact gtgatcctga cgaaattgac
     4741 tacgttatcc cagcaaacga cgatgctatc cgtgcggtta aattattaac tgctaaaatg
     4801 gcagatgcaa tcttagaagg tcaacaaggc gtttctaatg aagaagtagc tgcagaacaa
     4861 aacatcgatt tagatgaaaa agaaaaatca gaagaaacag aagcaactga agaataatca
     4921 actgttgaat ctgacttaga tatagtttaa atgggtgata agatattaat gcttatcacc
     4981 ttttttaaaa agaaaatcga ggcaaattac aaatattcaa ttagagtatt ggcaatcttg
     5041 cctataataa tgctaaaatc ataatatata aaatgataac ttattggagg aataatgaat
     5101 ggcaactatt tcagcaaaac ttgttaaaga attacgtgaa aaaactggcg cgggtatgat
     5161 ggattgtaaa aaagcgctaa ctgaaactga tggtgacatc gataaagcga ttgactacct
     5221 acgtgaaaaa ggtattgcta aagcagctaa aaaagcagac cgtattgcgg ctgaaggttt
     5281 agtacatgta gaaactaaag gtaacgacgc agttatcgtt gaaatcaact ctgaaacaga
     5341 ctttgttgct cgtaacgaag gtttccaaga gttagttaaa gaaatcgcta atcaagtatt
     5401 agatacaaaa gctgaaactg ttgaagcttt aatggaaaca actttaccaa atggtaaatc
     5461 agttgatgaa agaattaaag aagcaatttc aacaatcggt gaaaaattaa gtgttcgtcg
     5521 ttttgctatc agaactaaaa ctgataacga tgctttcggc gcttacttac acatgggtgg
     5581 acgcattggt gtattaacag ttgttgaagg ttcaactgac gaagaagcag caagagacgt
     5641 tgctatgcat atcgctgcaa tcaaccctaa atatgtttct tctgaacaag ttagcgaaga
     5701 agaaatcaac cacgaaagag aagttttaaa acaacaagca ttaaatgaag gtaaaccaga
     5761 aaacatcgtt gaaaaaatgg tggaaggacg tttacgtaaa tacttacaag aaatttgtgc
     5821 tgtagatcaa gacttcgtta aaaaccctga tgtaacagtt gaagctttct taaaaacaaa
     5881 aggtggaaaa cttgttgact tcgtacgcta tgaagtaggc gaaggtatgg aaaaacgcga
     5941 agaaaacttt gcggatgaag ttaaaggaca aatgaaataa tctgtcataa agtaaaacaa
     6001 ggaagaagac acctttaatg ttgctttatt aaaatgtaaa tcattctaat aaaacgacaa
     6061 ctgtgtcttc tttacttgta tatgttacat atattcacga tagagaggat aagaaaatgg
     6121 ctcaaatttc taaatataaa cgtgtagttt tgaaactaag tggtgaagcg ttagctggag
     6181 aaaaaggatt tggcataaat ccagtaatta ttaaaagtgt tgctgagcaa gtggctgaag
     6241 ttgctaaaat ggactgtgaa atcgcagtaa tcgttggtgg cggaaacatt tggagaggta
     6301 aaacaggtag tgacttaggt atggaccgtg gaactgctga ttacatgggt atgcttgcaa
     6361 ctgtaatgaa tgccttagca ttacaagata gtttagaaca attggattgt gatacacgag
     6421 tattaacatc tattgaaatg aagcaagtgg ctgaacctta tattcgtcgt cgtgcaatta
     6481 gacacttaga aaagaaacgc gtagttattt ttgctgcagg tattggaaac ccatacttct
     6541 ctacagatac tacagcggca ttacgtgctg cagaagttga agcagatgtt attttaatgg
     6601 gcaaaaataa tgtagatggt gtatattctg cagatcctaa agtaaacaaa gatgcggtaa
     6661 aatatgaaca tttaacgcat attcaaatgc ttcaagaagg tttacaagta atggattcaa
     6721 cagcatcctc attctgtatg gataataaca ttccgttaac tgttttctct attatggaag
     6781 aaggaaatat taaacgtgct gttatgggtg aaaagatagg tacgttaatt acaaaataaa
     6841 tttagaggtg taaaataatg agtgacatta ttaatgaaac taaatcaaga atgcaaaaat
     6901 caatcgaaag cttatcacgt gaattagcta acatcagtgc aggaagagct aattcaaatt
     6961 tattaaacgg cgtaacagtt gattactatg gtgcaccaac acctgtacaa caattagcaa
     7021 gcatcaatgt tccagaagca cgtttacttg ttatttctcc atacgacaaa acttctgtag
     7081 ctgacatcga aaaagcgata atagcagcta acttaggtgt taacccaaca agtgatggtg
     7141 aagtgatacg tattgctgta cctgccttaa cagaagaacg tagaaaagag cgcgttaaag
     7201 atgttaagaa aattggtgaa gaagctaaag tatctgttcg aaatattcgt cgtgatatga
     7261 atgatcagtt gaaaaaagat gaaaaaaatg gcgacattac tgaagatgag ttgagaagtg
     7321 gtactgaaga tgttcagaaa gcaacagaca attcaataaa agaaattgat caaatgattg
     7381 ctgataaaga aaaagatatt atgtcagtat aaaactaata tacaatgaca tattaaaatg
     7441 ccagtattaa acgataatgt aacatttaaa atgggcatgt ttaattaaat caaagatgca
     7501 tgtgataatt taaattcaca atgagcataa aaatggtgtt taaacaagtt aattaaacat
     7561 atactttata aataataggc attaggtata ttgctataat aaagttatgt aatttttaac
     7621 ctcagtatgt atgtcacatt tctggtgtaa actgtaccga gtcagacttt ggtacagttt
     7681 ttttatttgc ttattcaatg cattaaatga gtatgataaa atgataatga ttgtttagta
     7741 acttatacta tatgacagag atgatcaggc tcggaggaaa gaccatgttt aaaaagctaa
     7801 taaataaaaa gaacactata aataattata atgaagaatt agactcgtct aatatacctg
     7861 aacatatcgc tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta
     7921 gaattaaagg tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg
     7981 atattggtgt taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg
     8041 aaagtgaagt aaattatatt atgaatttgc ctgtcaattt cttaaagaca ttcttaccgg
     8101 aactaattga aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat
     8161 caacgataga agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat
     8221 taatatttgc aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt
     8281 ttgacgagct tcatcaacaa ggtttaaata gtgatatcat agatgaaaca tatataaaca
     8341 atcatttaat gacaaaagac tatcctgatc cagagttgtt aattcgtact tcaggagaac
     8401 aaagaataag taatttcttg atttggcaag tttcgtatag tgaatttatc tttaatcaaa
     8461 aattatggcc tgactttgac gaagatgaat taattaaatg tataaaaatt tatcagtcac
     8521 gtcaaagacg ctttggcgga ttgagtgagg agtagtatag tatgaaagtt agaacgctga
     8581 cagctattat tgccttaatc gtattcttgc ctatcttgtt aaaaggcggc cttgtgttaa
     8641 tgatatttgc taatatatta gcattgattg cattaaaaga attgttgaat atgaatatga
     8701 ttaaatttgt ttcagttcct ggtttaatta gtgcagttgg tcttatcatc attatgttgc
     8761 cacaacatgc agggccatgg gtacaagtaa ttcaattaaa aagtttaatt gcaatgagct
     8821 ttattgtatt aagttatact gtcttatcta aaaacagatt tagttttatg gatgctgcat
     8881 tttgcttaat gtctgtggct tatgtaggca ttggttttat gttcttttat gaaacgagat
     8941 cagaaggatt acattacata ttatatgcct ttttaattgt ttggcttaca gatacagggg
     9001 cttacttgtt tggtaaaatg atgggtaaac ataagctttg gccagtaata agtccgaata
     9061 aaacaatcga aggattcata ggtggcttgt tctgtagttt gatagtacca cttgcaatgt
     9121 tatattttgt agatttcaat atgaatgtat ggatattact tggagtgaca ttgattttaa
     9181 gtttatttgg tcaattaggt gatttagtgg aatcaggatt taagcgtcat ttcggcgtta
     9241 aagactcagg tcgaatacta cctggacacg gtggtatttt agaccgattt gacagcttta
     9301 tgtttgtgtt accattatta aatattttat taatacaatc ttaatgctga gaacaaatca
     9361 ataaacgtaa agaggagttg ctgagataat ttaatgaatc tcagaactcc
//
LOCUS       DALAAR010000094         9006 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000094 DALAAR010000000
VERSION     DALAAR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.096"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(408..506)
                     /locus_tag="O2458_002389"
     CDS             complement(408..506)
                     /locus_tag="O2458_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888235.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="O2458_002390"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="O2458_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCZ3888236.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="O2458_002391"
     CDS             complement(1314..1631)
                     /locus_tag="O2458_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCZ3888237.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O2458_002392"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O2458_002392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCZ3888238.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="O2458_002393"
     CDS             complement(3487..4011)
                     /locus_tag="O2458_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3888239.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="O2458_002394"
     CDS             complement(4149..4250)
                     /locus_tag="O2458_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888240.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="O2458_002395"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O2458_002395"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O2458_002396"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O2458_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCZ3888241.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O2458_002397"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O2458_002397"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCZ3888242.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O2458_002398"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O2458_002398"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCZ3888243.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
BASE COUNT         2849 a         1963 c         1207 g         2987 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatg
//
LOCUS       DALAAR010000095         8962 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000095 DALAAR010000000
VERSION     DALAAR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.097"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            201..551
                     /locus_tag="O2458_002399"
     CDS             201..551
                     /locus_tag="O2458_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888244.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            638..949
                     /locus_tag="O2458_002400"
     CDS             638..949
                     /locus_tag="O2458_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCZ3888245.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            964..1470
                     /locus_tag="O2458_002401"
     CDS             964..1470
                     /locus_tag="O2458_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCZ3888246.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            1522..3129
                     /locus_tag="O2458_002402"
                     /pseudo
     CDS             1522..3129
                     /locus_tag="O2458_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(3120..3380)
                     /locus_tag="O2458_002403"
     CDS             complement(3120..3380)
                     /locus_tag="O2458_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ3888247.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(3352..4338)
                     /locus_tag="O2458_002404"
     CDS             complement(3352..4338)
                     /locus_tag="O2458_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ3888248.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            4435..6444
                     /gene="mecA"
                     /locus_tag="O2458_002405"
     CDS             4435..6444
                     /gene="mecA"
                     /locus_tag="O2458_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCZ3888249.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(6490..6918)
                     /locus_tag="O2458_002406"
     CDS             complement(6490..6918)
                     /locus_tag="O2458_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ3888250.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(7015..7758)
                     /locus_tag="O2458_002407"
     CDS             complement(7015..7758)
                     /locus_tag="O2458_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ3888251.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(8595..>8962)
                     /locus_tag="O2458_002408"
     CDS             complement(8595..>8962)
                     /locus_tag="O2458_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HCZ3888252.1"
                     /translation="LWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLFSYGSGAVG
                     EIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFERELTQDPY
                     SKVYLYSIEDHIRTYKIEK"
BASE COUNT         3266 a         1477 c         1340 g         2879 t
ORIGIN      
        1 cttataaata atgagttgat tgtttgtcaa aataggcgga atttcaggat ttccttggca
       61 taccaacgtt tgtagcgggt ttttatatgg aattttctct tattcccacc gggattcgta
      121 ataaatgaca aatcaaatat atattatctc aatgatgtat atgaactaca tcactattac
      181 actatctagg aggcaataat atgaaaacca tcactcaaga actcaaacaa tatataaccc
      241 acctattcca actatctaat aacgaagctt gggaatgtga agcgctggag gaagcagcag
      301 aaaatattct accggaacgt tttattaatg attctccact ggtacatctc acactcgaga
      361 cttataccta ctataatgat gaactacatg agctaagtat ctatccattt ctaatgtatg
      421 ccaataacca actcatcagt gtcggatact tagaccattt tgacatggac tttctgtacc
      481 ttacagatac tcaaaatatc attattgatg aacgtcattt actaaaacaa ggaggtcagg
      541 accatgaata attggatcat agttgctcaa ttatccgtca cagttatcaa cgaaatcatc
      601 gacatcatga aagaaaagca gaagggaggg aaatagcatg aaaatcaatc gatacatcac
      661 aagaggaatt aatgaaagta ttccactaga ccttcaaatc ttactttggc acatggtaga
      721 aaaaaaagat aaccagcctc atacagatta cctacacatt tttaaattac aagaagatga
      781 gaatatactc tcaatcacac atgaacaaga acaacccaca tacaaattgg aatatcacta
      841 tacaaactat gaaaaaaatc aaaatgcatt acctaagaaa gtctacgtca ttcgtgaaga
      901 tgacgtggac gctttttatt atgtgatgct tttaccagaa gaatactaaa ggaagtgacg
      961 atgatgaata caatcaaaag tacgatacac acagaagcca tatttagcga tgatgaacag
     1021 caccgctatt tactcaagaa aacatgggat gaaaagaaag ctgcatgcac agtgataacg
     1081 atgtatcctc atttagacgg tgtattatca ctcgatctca caactgttct tatcctcaac
     1141 caattagcca attcagaacg atacggtgct gtatatcttg taaatctatt ctctaatatt
     1201 agaacacccg aaaacctcaa acatatcaaa aatccatacg atgagcacac tgatattcat
     1261 ttgatgaaag cgattagtga aagtgacaca gtgattcttg cttatggtgc ctatgcgaag
     1321 cgaccagttg ttatcgaccg tgtcgaacaa gtgatggaaa tgttaaaacc tcataaaaag
     1381 aaagtaaaaa agctcatcaa tccagtaaca aatgaaatta tgcatccact caaccctaag
     1441 gcacgtcaaa aatggatttt gaaatcatag caaatacatg gaggtcaata tggaaacaaa
     1501 acaaatcaac atcgttttac tgtaaatgat attgttgaaa caattcctga caatgaattc
     1561 gacgaattca gacatcaccg tggtgcaact tcgtaccatc ctaaaatgat gttaaaagtg
     1621 attctatatg cctacacaca atctgtattc tcaggtcgta aaatagaaaa aatgcttaat
     1681 gatagcatcc gaatgatgtg gctatcacaa aatcaaaaac cttcttataa aacaattaat
     1741 caatttagag taaatccaaa agtagatgct ttattagaat ctttatttat tcaattttac
     1801 agtcagtgtg taaaacaaaa tcttatagat gataaagcta tttttattga tggtacaaaa
     1861 attgaagcaa atgccaatcg atatacattt gtatggaaaa agagtattca aaaccatgaa
     1921 tcaaagatga atgaggattc taaagccctc taccatgaat tggtaaccaa taaaatcata
     1981 ccggaaatta aagaagatca tgataatgaa ttaacaaaag aagaaataga tttgattggt
     2041 agtcacttag ataaagaaat cgaagattta aaccaacata tcaacaatga aaaatgtact
     2101 aaaacaagaa aacaaatacg tctcaaaaga actaaaatca aaaaatacaa aaagcaaatc
     2161 aatgattatt ttgagcgaaa gtatcgatac gaatttcaaa aatctatttt aaaggataga
     2221 aatagttatt ctaagacaga ttatgatgcg acatttatga gaatgaaaga agatcacatg
     2281 aaaaatggac aacttaagcc agggtataat ttacaaatag caacaaattc ccaatttgtt
     2341 ttatcttata atgtgtatca aaatccaacg gatactagaa cgatgattcc atttttaaat
     2401 tcaattcaag agacctacgg tcatttacct gaatatattg tagctgatgc aggttatggt
     2461 agtgaatcaa attataaggc aattatagat gactttaatc gaacgccact cataacatat
     2521 ggaatgttta taaaagataa aactaaaaaa tataaaagtg acatctttaa tactcaaaat
     2581 tggaactatg acgaaattaa tgacgaattc atttgtccga ataataaacg gctaggtttt
     2641 aaaagatatg cctatcgtca tgataagtat ggttataagc gagacttcaa attatatgaa
     2701 tgtgatgatt gttcagaatg tcctctgaaa aatcaatgta tgaacttcaa ttcaaaaaca
     2761 aacaaaaaaa taatgaagaa ttataactgg gaatatttta aatcccaaat taacaaaaag
     2821 ctttcagaac cagaaacaaa aaatatctac agtcaaagaa aaattgatgt ggaacctgtt
     2881 tttggattta tgaaggctat tttgggtttc actcggatgt ctgtccgagg actcaataaa
     2941 gtcaaaagag aacttggttt tgtattaatg gcacttaata taagaaaagt agtagctcaa
     3001 cgagctgaaa ataatcaaaa aatttataaa aaagacaatt tctatattat ttcaatagaa
     3061 attgtctttt ttcacttatc caagaacttt atgtcccgga ctcattatga tttatttttt
     3121 tattgctgac ttgccattaa ttctgcttgc tctacaactt gttccacagc cattttttga
     3181 agatctggtg gatagccata tttctttaat aggcgtctaa cttgaactct cattttagct
     3241 ttagcactgt ctcgtttaga ccaatcaaca ctcatgttat ccttaactgt tttagttaac
     3301 tcatgtgcaa tggctcttaa ctctttatcg cccaaagctt ctttagctgt ttcatgtgaa
     3361 gctcgatata ctacatacaa tatacgcaac aatacgcttg tttcgattag gtttaggcat
     3421 tgaatcataa agtgcaatat acttaacacg ttctttaata tttgaattaa aacctagtaa
     3481 atattgtgct gccacattat ttatgtgctg agattttaaa atagagcatt ttaatatcga
     3541 ttcaccataa cgtatatgtt catggcgatt caaaattttt aaaacgtttc tatcacatac
     3601 tttttcacag tcattgtcca tcattgtttt acttatatat agtgcaggat taaaccagaa
     3661 tatcatttta aaaacaacat aaagctggtt gaatattaag tcatgacttt tcacatgtga
     3721 tagttcatgt agaataatat attcaatttc tttgtcattc atggtttcga ctacgacagt
     3781 tggtagtaca atttgggatt tcactaaacc aaataccatc ggattatcaa tgtttgaact
     3841 ataactaatt gttatatgct ttttgtagaa ctgcatctta ctttgacata ctttaagtcg
     3901 ttcattaaga tatgacgatt ccaatgacga acttttaata acatcaattt gtcggaatgc
     3961 cttaatcata taaaataagc acaacaaact accaaatacc catatcaaaa gaatcatata
     4021 cgttatattt gaggtctcaa actgattaac attaattgct aagtctttcg taacagatga
     4081 ttgttgacca tctaacatat gactaaccga agaagtcgtg tcagatacat ttcgattcat
     4141 catatctttt gaaaatgtaa aattcgatat tttgtaaaat ggtattaatg gaattaacgt
     4201 ggagacgagc actaataacc aaatcttatg tgacataata ttttgagtat attttatata
     4261 gagcattctc actaaaaaaa ttacacatat cgtgagcaat gaactgatta tacttaacat
     4321 taaaaaagat gataacacct tctacacctc catatcacaa aaattataac attattttga
     4381 cataaatact acatttgtaa tatactacaa atgtagtctt atataaggag tatattgatg
     4441 aaaaagataa aaattgttcc acttatttta atagttgtag ttgtcgggtt tggtatatat
     4501 ttttatgctt caaaagataa agaaattaat aatactattg atgcaattga agataaaaat
     4561 ttcaaacaag tttataaaga tagcagttat atttctaaaa gcgataatgg tgaagtagaa
     4621 atgactgaac gtccgataaa aatatataat agtttaggcg ttaaagatat aaacattcag
     4681 gatcgtaaaa taaaaaaagt atctaaaaat aaaaaacgag tagatgctca atataaaatt
     4741 aaaacaaact acggtaacat tgatcgcaac gttcaattta attttgttaa agaagatggt
     4801 atgtggaagt tagattggga tcatagcgtc attattccag gaatgcagaa agaccaaagc
     4861 atacatattg aaaatttaaa atcagaacgt ggtaaaattt tagaccgaaa caatgtggaa
     4921 ttggccaata caggaacagc atatgagata ggcatcgttc caaagaatgt atctaaaaaa
     4981 gattataaag caatcgctaa agaactaagt atttctgaag actatatcaa acaacaaatg
     5041 gatcaaaatt gggtacaaga tgataccttc gttccactta aaaccgttaa aaaaatggat
     5101 gaatatttaa gtgatttcgc aaaaaaattt catcttacaa ctaatgaaac agaaagtcgt
     5161 aactatcctc taggaaaagc gacttcacat ctattaggtt atgttggtcc cattaactct
     5221 gaagaattaa aacaaaaaga atataaaggc tataaagatg atgcagttat tggtaaaaag
     5281 ggactcgaaa aactttacga taaaaagctc caacatgaag atggctatcg tgtcacaatc
     5341 gttgacgata atagcaatac aatcgcacat acattaatag agaaaaagaa aaaagatggc
     5401 aaagatattc aactaactat tgatgctaaa gttcaaaaga gtatttataa caacatgaaa
     5461 aatgattatg gctcaggtac tgctatccac cctcaaacag gtgaattatt agcacttgta
     5521 agcacacctt catatgacgt ctatccattt atgtatggca tgagtaacga agaatataat
     5581 aaattaaccg aagataaaaa agaacctctg ctcaacaagt tccagattac aacttcacca
     5641 ggttcaactc aaaaaatatt aacagcaatg attgggttaa ataacaaaac attagacgat
     5701 aaaacaagtt ataaaatcga tggtaaaggt tggcaaaaag ataaatcttg gggtggttac
     5761 aacgttacaa gatatgaagt ggtaaatggt aatatcgact taaaacaagc aatagaatca
     5821 tcagataaca ttttctttgc tagagtagca ctcgaattag gcagtaagaa atttgaaaaa
     5881 ggcatgaaaa aactaggtgt tggtgaagat ataccaagtg attatccatt ttataatgct
     5941 caaatttcaa acaaaaattt agataatgaa atattattag ctgattcagg ttacggacaa
     6001 ggtgaaatac tgattaaccc agtacagatc ctttcaatct atagcgcatt agaaaataat
     6061 ggcaatatta acgcacctca cttattaaaa gacacgaaaa acaaagtttg gaagaaaaat
     6121 attatttcca aagaaaatat caatctatta actgatggta tgcaacaagt cgtaaataaa
     6181 acacataaag aagatattta tagatcttat gcaaacttaa ttggcaaatc cggtactgca
     6241 gaactcaaaa tgaaacaagg agaaactggc agacaaattg ggtggtttat atcatatgat
     6301 aaagataatc caaacatgat gatggctatt aatgttaaag atgtacaaga taaaggaatg
     6361 gctagctaca atgccaaaat ctcaggtaaa gtgtatgatg agctatatga gaacggtaat
     6421 aaaaaatacg atatagatga ataacaaaac agtgaagcaa tccgtaacga tggttgcttc
     6481 actgttttat tatgaattat taataagtgc tgttacttct cccttaaata caatttcttc
     6541 attttcattg tatgttgaaa gtgacactgt aacgagtcca ttttcttttt ttatggattt
     6601 cttatttgta atttcagcga taacgtacaa tgtattacct gggtatacag gtttaataaa
     6661 tttaacgtta ttcatttgtg ttcctgctac aacttcttct ccgtatttac cttcttctac
     6721 ccataattta aatgatattg aaagtgtatg catgccagat gcaatgatac ctttaaatct
     6781 actttgttct gctttttctt tatctatatg catatattga ggatcaaaag ttgttgcaaa
     6841 ttggataatt tcttcttctg taatatgaag gctttttgtt ttgaatgttt ctcctactat
     6901 aaaatcatcg tatttcatat atgtctctct ttcttattca aattaatttt ttagtatgta
     6961 acatgttaaa ggtaagtcta ccgtcactga aacgtaagac tcacctctaa ctttctattg
     7021 agacaaatgc accattttat ctgcattgtc tgtaaagata ccatcaactc cccaattagc
     7081 aagttggttt gcacgtgctg gtttgtttac agtccatacg ttcaattcat aacccgcttc
     7141 ttttaccatt tttacttttg ctttagtaag tttggcatct tcagtgttta ctattttagc
     7201 attacagtaa tctaaaagtg ttctccagtc ttcacgaaac gaagttgtat ggaatataac
     7261 tgctctgtta tattgtggca tgatttcttc tgcaagttta acaagcacaa cattaaagct
     7321 tgaaatgagc acttcttgat tctgatttaa gtttgttaat tgttcttcca cttgcttaac
     7381 catactttta gaaagtgcta gtccattcgg tccagtaata ccttttaatt ctacatttaa
     7441 attcatatta tattcatttg ctatttttac tacatcatcg aaagttggca aatgttcatc
     7501 tttgaatttt tcaccaaacc aagatcctgc agaagcatct ttaatttcat cataattcaa
     7561 ttcagttatt tccccggaca tatttgtagt ccgttctaaa taatcatcat gaatgataat
     7621 cagttgttca tcttttgtaa ttgcaacatc taactccaac cagtttatac cttctacttc
     7681 tgaagcagct ttaaatgatg caattgtatt ttccggagct ttactaggta atcctctatg
     7741 tccatataca gttagcatat tacctctcct tgcattttta tttttttaat taacgtaact
     7801 gtattatcac attaatcgca cttttatttc cattaaaaag agatgaatat cataaataaa
     7861 gaagtcgata gattcgtatt gattatggag ttaatctacg tctcatctca tttttaaaaa
     7921 atcatttatg tcccaagctc cattttgtaa tcaagtctag tttttctgta ccccttatct
     7981 gcaattttac ttaggattgc ttttaactta ccccttatca gcaattttac tgagaactgc
     8041 ttttaacgca cctcttatct gcaattttgc ctagaactgc ttttaacgta cctcttatct
     8101 gcaattttac tgagaactgc ttttaactta ccccttatca gcaattttgc atggaattgc
     8161 ttttaacgta cctcttatct gcaattttac ttagaactgc ttttaacaaa cctcttatct
     8221 gcaattttac ttagaactgc ttttaacgta cctcttatct gtaattttac tgagaactgc
     8281 ttttaacaaa cctcttatct gcaattttac ttagaattgc ttttactatt cctcttatta
     8341 gtataatctc agtaagaatg cgtataaaaa tgaaaattac aaccgatttt gtaagtgctg
     8401 acgcctgagg gaatagtatg tgcgagagac taatggctcg agccataccc ctaggcaagc
     8461 atgcacgtac aaaatcgtaa gataaaaaaa taagcatatc actgtaaact ttaaaaaatc
     8521 agtttagtga tatgcttatt tatttcgagt taggatttat gtcccaagct catcaagcac
     8581 aatcggccac tagtttattt ctctatctta tatgttctga tatggtcttc tatactgtat
     8641 aagtatactt ttgaatatgg atcttgtgtc aattcacgtt cgaaatcaaa ttcttgatta
     8701 tcaaatctgt taaagaatgt ttcgtattct tcgactgata attgctctct agattctagc
     8761 atatttaagt gtttctcttt atctaatgct ttgtcatatc ctttaacgat tgaaccacta
     8821 aagatttctc ctactgctcc tgaaccataa ctaaatagac atactttctc ttctggttgg
     8881 aatgtgtggt tctgtaataa cgaaattaaa cttaagtata atgatcctgt ataaatgtta
     8941 ccaacatctc tattccataa ta
//
LOCUS       DALAAR010000096         8715 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000096 DALAAR010000000
VERSION     DALAAR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.098"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(169..1830)
                     /locus_tag="O2458_002409"
     CDS             complement(169..1830)
                     /locus_tag="O2458_002409"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCZ3888253.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(1827..2243)
                     /locus_tag="O2458_002410"
     CDS             complement(1827..2243)
                     /locus_tag="O2458_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCZ3888254.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(2336..2443)
                     /locus_tag="O2458_002411"
     CDS             complement(2336..2443)
                     /locus_tag="O2458_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888255.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(2518..3528)
                     /gene="adhP"
                     /locus_tag="O2458_002412"
     CDS             complement(2518..3528)
                     /gene="adhP"
                     /locus_tag="O2458_002412"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCZ3888256.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(4027..4548)
                     /locus_tag="O2458_002413"
     CDS             complement(4027..4548)
                     /locus_tag="O2458_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCZ3888257.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(4576..5871)
                     /locus_tag="O2458_002414"
     CDS             complement(4576..5871)
                     /locus_tag="O2458_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ3888258.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(5911..6387)
                     /locus_tag="O2458_002415"
     CDS             complement(5911..6387)
                     /locus_tag="O2458_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888259.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(6556..7092)
                     /locus_tag="O2458_002416"
     CDS             complement(6556..7092)
                     /locus_tag="O2458_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCZ3888260.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(7303..8241)
                     /locus_tag="O2458_002417"
     CDS             complement(7303..8241)
                     /locus_tag="O2458_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ3888261.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(8511..>8715)
                     /locus_tag="O2458_002418"
     CDS             complement(8511..>8715)
                     /locus_tag="O2458_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3888262.1"
                     /translation="TLKNFCLTLFRYAFFILWTVIFSYALLSMSYQNIIVYFAWITAI
                     MGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         2758 a         1695 c         1129 g         3133 t
ORIGIN      
        1 ttgatttatg tctcaggctc tcttatgcag ttgtgcagag catccaactg tgttcctctt
       61 aaccttattc acatactaag cattccccaa tatgtcacat acctaaaaaa acactccaac
      121 acaaagtatc tttcgagaaa ctctacgttg gagtgtatat aaatgttctt acattgattc
      181 aggtgcgctt acaccgacca ttgccaatgc atttttcaat gtaattctga ccgcttcaat
      241 catagcaaca tgtgcttttg ttttttcaat atcatctgtt aacacttttt cagcattata
      301 gaatttatgg aaatgagaag ctaaatcttg aatataatta gtaattctat gtgccgatct
      361 atgctcagca gcactttcaa ttgtaggttc gaaatcagct acttttttca acaattcaat
      421 cgctttttca ttagtaatcg ttgtaaaatc attcgcagca gtcacttcaa taccttgctc
      481 tttcgcttgt tttaaaattg aacaaatacg cgcatgtgca tattgagcat agtaaactgg
      541 attgtcttga gattgctctt tcgctaattc catatcaaaa tcaaagtgac tatcaggact
      601 acgcatagtt aagaaataac gtgcagcgtc aacgccaact tcgtccataa tttctcttaa
      661 tgtaatcgca ttaccagtac gtttactcat cttcacttct ttaccatttt ccattaaacg
      721 aaccatttgc atgatttgaa tttctaaacg attactatct acaccaaacg tttcaagaga
      781 tgctttcaaa cgattaatat aaccatgatg atcagcacca aataaatcga ttaaaatgtc
      841 attaccacgt tttactttat cgaagtggta cgcaatatct ggtaagaaat acgtatatgt
      901 accgtcattt ttaattaata ctctgtcttt gtcgtcttta aaatcagttg tacgtaacca
      961 tgtagcgcca tcagcttcat acgtataacc taattctttc atttttgcta aaacttcaag
     1021 aatttctcct ttttcatata aagatgtttc actaaaccaa ttatcaaaat gcgtattgaa
     1081 ctctgctaaa tcatttttca atttagccat ttcgtattct acgcctaatt ttctaaattc
     1141 tttcaaacgt gcttcttcag aataatcttt aatttcaggg tgtttctctg ctaaatcttt
     1201 acctatttca ataatatctt ttccattata gccatcttct ggcattgaat aactattgtc
     1261 acctaaagct tcaaagaaac gtgtttcaat cgaacgcgct aagttagtaa tttgattacc
     1321 agcatcatta atataatatt cacgtgttac attatagcca gctgcagtta aaatattagc
     1381 taaagcatca ccaactgctg catttctagc atgaccaata tgtaaatctc ctgtagggtt
     1441 agctgaaaca tactcaagca atacattttg accttttgat tcatttacat gtccaaattg
     1501 atcacctttt tcaattgctt caggaataat tgctgttaaa tactgattat ctaagtaaaa
     1561 attaatgaat cctggaccag caatgtcaat ttgttttaca tgtgcttttt cagtatctaa
     1621 gttatcaaca atcgcttgag caatttcacg aggattacgc tttgcaatct tagtcagtac
     1681 catcgcaata ttagtagcat aatctccatt ttttgtatct ttaggaactt caattttaat
     1741 atcaggaatc tcatctgcta atcctgcttt gttaatactt gctgcaattt cttctactaa
     1801 tgtttgtttc acttgatcaa taatattcat tcgcctatct ccttatagtt aatttcatat
     1861 tgataagaac ccattttttc attatcttga tataattcat aatgaatctt tagcttgcca
     1921 ccgtctccac ttacgaaatg taaaatgcgt aatgttttaa cttctagtgg aattcgtcca
     1981 gccgatatat cgtaaaaagt tgttgtcgtt tgtccttcaa caaaatgcaa attcatatta
     2041 atgtcgcctt tacgaatcaa cttaacgcta tcatcatcca cttttattgt aacattaact
     2101 gttgcatcct caatttgttc tacataccga ataaagttag attgcctttg ttgccaagtt
     2161 ccttcagtag taaattcaaa tttttctttt tcgttgtgct gtttcaacac ttgctttgtt
     2221 tgaatactta cttttttatc caatttacta ctcaccttaa tcttcgcata atagaaatat
     2281 tatagcataa aataaggtca tcttgttatg ccttcttaaa gctaaatagg ttcaattaat
     2341 taacaattcc atacttaatc aacgcttcat atatgtaagc cattttataa aatacaaaca
     2401 acacctcaaa accaaaaaac acgagatttt cactatcttt catgaaaacc tcgtgtttga
     2461 ttataatgat agtgttcaga gcactacctt ctttatctat atagtcagtt gatattttta
     2521 aaatttaata accattctac cagttatagt accattttcc atttcttcaa aaatatcatt
     2581 gatttcttct aattttctta attgaacttt aggtgttact ttattttcag cagcaaattc
     2641 aaacgcttca cgtaagtctt gtcttgtacc aacaagtgaa cctactactt caataccatc
     2701 aagcactaat cttgggatat ctaagttcat tttatcaaca ggtaaaccaa cggcaacaac
     2761 tcttgcacca gcttttacaa catcaaccgc ttggttaaat ggcgtcttag caactgaagt
     2821 taccactgtt gcatctaatc ctttgttatc agttaatttc ataacttctg caactggatc
     2881 aacatcttta gaattaataa tagcatcagc acctaattct ttcgcgaatg ctaatttatc
     2941 atcattgatg tcgaaggcaa caattttagc ccccataacg tttttagcat attgtaaagc
     3001 taggttacct aaaccaccta taccaaaaac acctaaccat tgtccaggtt ttacattact
     3061 tacttttaca gctttataag ttgtcacacc tgcgcatgta atagaagacg ctgctgctgg
     3121 atctaatttt tcaggtactt tcacagcata gtctgcagta acaataactt gttcagccat
     3181 tgcaccatct actgtataac cagcattttt cacactacgg caaagtgttt cacgacctgt
     3241 tgtacaatat tcacatcttc cacagctttc gaacatccaa gcgatagaca cacggtctcc
     3301 aatttttaat gattctacat cttccgcaac ttcgatgact ttaccaatac cttcatgacc
     3361 taaagtaacg cctgtaacat caccaaaatc agcattctta acatgtaaat cggtatgaca
     3421 aacgccacaa tattccgttt gtaccaacgc ttcaccaggt tttaaagctc ttaacttttt
     3481 gtcctcaata cttactttgt gatctttcgt tacaactgct gctctcataa taatgtcctc
     3541 cttatagtca ctccagtatg tatgatatta attggctatt actttcaaaa aagaaaacga
     3601 ataattatag atgtaatttt tatttgtaat agacgccaga ttaaaacata ctactgtgac
     3661 cttgttttta gttagatatc aaccgttaac ttttaacgtt tccctagtta atatgttaaa
     3721 agaaatgaca taatcgatat gctaacgtgt aatttttact tcacatatat ttatttaaag
     3781 ataatttata atactcttat cttcactctt attataatgt gaattaattc acaaagtata
     3841 tagctatgat caaaaaaatg tgtcagttat tcaaattttt tattaactac agattaattt
     3901 aatttcaaaa aaattaaaaa aactcccaat caatctaaga cattattttc acctcacgat
     3961 ttacgtcgtc attgagaaat gaatcatatc tttatagatt aattgggaaa ttttagtttc
     4021 taatatttaa ttaaatgctt gatgtaaagc atcagaagaa tgcttccaca taccttcatc
     4081 atggttcatt aaaaagtctt ttaaaatagc tttattctcg tcaccaattt gatctatgat
     4141 aatatgatgt ttcattgatt tatccatcat attaacatgt tcaggcatac ttttataacc
     4201 acgacgaatt gctttattca ctaataaata gcacgctgtt aaaccagcgt aatatggtcc
     4261 ttgttcatta cgttctgttg ttacccaaca caaccaatat tctttagctt catcgccttc
     4321 aacagcagct ttatcagtaa tccatttcac acctttttca actttggcac gagcatgcat
     4381 gcctccaata tcaataaatg cttctttgtt agcaacatcg ataaaaactg gtgcaatatt
     4441 gtctaagcta attgaaccaa tatttgtacc tttatgacca tctagagggt cattttttat
     4501 gatattaaaa ttaaagcctt ttttctcaga caatgatata acctccatat ttaacgataa
     4561 caattttagc cctgtttaat taagctcatt aatcaaattt tcaatagctt ctctaattgt
     4621 cttatcttta atctttgcaa ccatttctct aggataatat aatttatagt cttggcgatt
     4681 aatgcctgta atactatgaa tgactaatga ttgattgctt atttctctaa tcgtaccatc
     4741 ttgtcttaat aaatgaatcg gtttgcgatt tgaccccggt cgatcatagt catatggcaa
     4801 atcagaaaat gcttcactca caaaataata atctggatta ataccacctg cttcaaacag
     4861 ttcttgcagt tctgatattg taataattga gccatcaaat ggaatatatt taaataagtc
     4921 tcgattaata aatcgacttg ctaaatcact taaaatagca tcatcttctt ttgtccattt
     4981 ttccaaataa tacgtaacta ccgcttcatc gagttcaaca tattgttcaa tcgtaactgt
     5041 ctcttcaaaa aatggaataa aatcatgtgg atgcaactta aattcatagc cttcattata
     5101 aagctgtttt gcgcgtttca aacaattatt aagcagcact tctccaccac gactaactgg
     5161 gtggaagtaa atttgccaat acatttgata acgactcata ataaagtttt caactgcatg
     5221 cataccactt tctttgatta gtacttcatc tttagaaggt cgcattaatc ttaaaatacg
     5281 ctccatatca aaagcaccat atgatacacc tgtgaaatac gcatcacgtt gtaaataatc
     5341 cattctatcc gcatcgattt gtgacgaaat catcgaaatg accaatttat tatgatgcgt
     5401 tttattaatt acttccgcaa cttctcttgg aaactcaggc gccactttac gtaatacagc
     5461 attcacctca gtatctccag taataatcgc ttgtgtgtat gcttcatggt ctgtattaaa
     5521 tattttttca aaactatgtg aaaatggacc atgtcctaaa tcatgtaata atgcagcaca
     5581 taatgccaac ggtctatctt tattgtccca tgcatcatga ccaataaatg actcatcaat
     5641 taatcgtcta actatttcat acacacctaa agaatgtcca aagcgactat gttctgctgt
     5701 gtgaaaagat aggtacagtg ttcctagttg tctaattcga cgtaaccttt ggaattcctt
     5761 agttttaatt aaatcccata tcaattgatc ttcaacatga atatatcgat gtatcgggtc
     5821 tttaaaaact ttttcttcta ctaattttaa atctacatat gcgttagtca ttattcccct
     5881 ccttttcgtt taatataata tttaatttac ttaaaatgct ttgtacataa gtgctaagtc
     5941 taacttttcg ccatacattt ctggctcata agagcgtaag attgtaaaac cttgctcttt
     6001 atagtaagct actgcttctt catttttatt atctacttct aagtaaacac cttcaaattt
     6061 atcttcaaaa cgtgataatc cttcatttaa caatgctgta ccataacctg tatgttgcga
     6121 ttctggttta acataatgag ctgataaata taattcttca ccgtaaataa agttagcaaa
     6181 gccaacgatg tcattacctt cttcaacgac taagaataat tgttcttgaa gtcttttctt
     6241 taaatgatgt tcattatatg aagcttctaa caagtgatta actgttgtcg cagcgtatat
     6301 atttaagtat gtattaaacc aagctttagt tgcgacatct ctaatttgaa caacatcttt
     6361 ttcagttgct tgtcttacct tgaacatgac tttctcccct tattaacaag ttttaataac
     6421 ggcattatac cacaacttgc tcaatactta ataaacaatg attgtctatt caatttatat
     6481 atttatattt tccgttaaaa ttaaaaataa aaaataacga agcaaaaaat cacttcgttt
     6541 agtatgaggt atgtcttatt gcaatatact attccactca gttgcacgtg ctaaggcata
     6601 gttgtctttc atgatgtcac caggcttttc agcagttcca ataatataac catttaaagt
     6661 ggcacctaaa aagtctaaac tatatttcat ttgcgtaatt gctggcttcg cttttatttt
     6721 tggacaatct ccaccaacta aaataactct aaaatccttt tcggccattt gtgccttaaa
     6781 attaggatat cgtttatctt gtaatgtttc tgaccaatgt tcgataaatg ctttcaatgg
     6841 tgctgaaatg ctataccaat acactggtga tgcaaaaata attgtatcac tagccaatat
     6901 tttatctaga atcggcaaat agtcatcgtc atatgaagta atagtctctg ctgtatgtct
     6961 cacgtcacgt atcggtttaa actgatgttg tgtcacgtca atccattgat actctaaatc
     7021 ttgcaaagcg aattttgtta attgtgcagt attaccgttt ggtctactcc caccaaacaa
     7081 aacagtaatc attttagcct aacctcactt ttgattaata aatatctgtg tttttcgtta
     7141 cctaattata ctatcataag ctttgcctac cgaatagtaa aacgcttaca acttttatat
     7201 aaatttgacg aaatttcgtc atgccttata taacgtcgtt tgtgatacgg ggctaattca
     7261 tgatgaaatt agatacatat atcaccatta aatacaattc atttagtctt caatcggaaa
     7321 cagttcatcg atatattgaa tctcatcatc tgataaaacg atatctgcag ctttaatatt
     7381 ttcaacgact tgttctgcac gttttgcacc aggaataatc acatcgatag ctggtctcgt
     7441 taaataaaat gctaatacaa tgttcgcaat tgaagtttga tgtgctgcag ctatgctttc
     7501 caaagctttt acgcgacgca cattttcttc aaatacacct ggtttaaaat cacgacgtgt
     7561 agtacgatgg tcactaaatt tagtgttctc atcatatttt ccagctaaaa taccggatgc
     7621 taatgggaaa tatggaataa atgtgatttg gtgatcaaca caatattgta atactgcctc
     7681 attttcgcga tgcaataaat tatattctaa ctgtacaaca tcaacgtaac catctttatt
     7741 tgcttcttta agttgatcta atgtgaaatt tgatacacca attgctttaa tcttcccttg
     7801 ttccttaagc tcttgtaatg ctgcaactgc ttgatctttc ggagtgttgt tatccggaaa
     7861 atgaatataa tataaatcga tataatcagt ttgtagacgt ttcaaactat tctcaacttg
     7921 ttgttttaaa tattccggtt gattgttctg atgtacttct tgattttcat caaattcatg
     7981 agaccctttc gtagcaattt taatttgctc tcgcggatat tctttaacaa cttctccaac
     8041 caattcttct gatcgttctg gcccataaat atatgccgta tctaataaat taataccatg
     8101 attaatggct tgacgaacaa catcttttcc ttgttcttca tctaagttcg gatataaatt
     8161 atgcccacct actgcgttcg tcccaagtgc gattggaaac acttcaacat cagatttacc
     8221 taagtttaca aattgcttca ttagacccag ctccttatct ttataacttt tccactatca
     8281 cactatcatg atttaataca acttccaaat gttagaatta gagcttgaat ttaagcggtg
     8341 taataggaaa acgttatata aaactcagtt aaaataagta ttgcttaaat tgatatgcat
     8401 atagcgcgat tgctcgttga cctttaaatt acatgtactt ttgaatcaga aattgaaaaa
     8461 catttaagaa cgacaatatt aaagccattg caaatttaat ttggtaattt ctattggttt
     8521 ttaagtatga catgaatgtt tttatctata attaaagcta tattcactag aaaaaaaccc
     8581 attattgctg taatccaagc aaaatatact attatgtttt gataactcat tgataataga
     8641 gcatatgaga aaatcactgt ccataaaata aaaaaagcat atctaaacaa tgttaaacaa
     8701 aaatttttta gtgtt
//
LOCUS       DALAAR010000097         8682 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000097 DALAAR010000000
VERSION     DALAAR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.099"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            114..269
                     /locus_tag="O2458_002419"
     CDS             114..269
                     /locus_tag="O2458_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888263.1"
                     /translation="MMVKKLLKAIEQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            277..798
                     /locus_tag="O2458_002420"
                     /pseudo
     CDS             277..798
                     /locus_tag="O2458_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            937..1467
                     /locus_tag="O2458_002421"
     CDS             937..1467
                     /locus_tag="O2458_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ3888264.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1730..4531
                     /gene="clfA"
                     /locus_tag="O2458_002422"
     CDS             1730..4531
                     /gene="clfA"
                     /locus_tag="O2458_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCZ3888265.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
     gene            4752..6278
                     /gene="vwb"
                     /locus_tag="O2458_002423"
     CDS             4752..6278
                     /gene="vwb"
                     /locus_tag="O2458_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCZ3888266.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            6629..7651
                     /gene="emp"
                     /locus_tag="O2458_002424"
     CDS             6629..7651
                     /gene="emp"
                     /locus_tag="O2458_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCZ3888267.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            7986..8456
                     /locus_tag="O2458_002425"
     CDS             7986..8456
                     /locus_tag="O2458_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888268.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         3357 a         1283 c         1553 g         2489 t
ORIGIN      
        1 attgaagaag acgtgttatt aatttggaaa atactggtca aagatgggag ctcttaaaag
       61 cgttattgta ttttttagtc aatacaaata gattgccgta ataataatcg tacttgatgg
      121 ttaaaaaatt acttaaggct atagagcaaa actttttata tgagcagtcg aatataacgt
      181 ttaaaatgat tgtttttgga tataaacgat taagtaaaat gctttttcag tttgaaatta
      241 atcatataaa tttcttatgg gagggttgat atcttaatga ttaacattat ttcagctata
      301 ggatctattg gaacatttat tatggcttta ttttattttg tatcagtttc agttcaactt
      361 tatcaaatga aaattagctt tctgccagct ttaggtttta accaaatttt attagaaagg
      421 gaggaggatc aacttaatat aatgaattcg gcaacagaag agcatcatca taaagattat
      481 attaaactat ataatttagg tggcggtgct gctaaaaaaa ttgcaataga ggttttattg
      541 gggaaggata aagtcattca gaaaaaatac gtgcatattt tacctagtaa agaagggtac
      601 atgttaccaa ttaataaaaa tgtgtacgaa gaattagaaa gaacgattga gaacaatggt
      661 catgaagctg atttgaatgt acgtatgact tattatcata atgtaagtcg caaacaatag
      721 gaagttatat taaaaggtca aatcgaccgt tttaatactt ataataataa agaaatttat
      781 gatttgcagt ttatctaaaa attgatttaa gagggtagtt gtttattgcg aaaaatatca
      841 ttcaatttta atgaaataat ggcgtcatta ctataaaata ttactttatg ttgtaatgca
      901 tttttctata agatagaact aaaaggaggg gcaaagatgc aaattagaca aatacatcaa
      961 catgactttg ctcaagtgga ccagttaatt agaacggcat ttgaaaatag tgaacatggt
     1021 tatggtaatg aatcagagct agtagaccaa attcgtctaa gtgatacgta tgacaatacc
     1081 ttagaattag tagctgttct tcaaaatgaa gttgtagggc acggtttact aagtgaagtt
     1141 tatcttgata acgaggcaca acgggaaatt ggattagtgt tagcacctgt atctgttgat
     1201 attcatcatc aaaataaagg tattgggaag cgattgattc aagcattaga acgagaagca
     1261 atattaaaag gatataattt tatcagtgta ttaggatggc cgacgtatta tgccaatcta
     1321 ggatatcaac gcgcaagtat gtacgacatt tatccaccat atgatggtat accagacgaa
     1381 gcgtttttaa ttaaagaatt aaaagtgaac agtttagcgg gaaaaacagg taccataaat
     1441 tacacatctg cttttgaaaa aatatgattt caagctagga ttacattagg tagagttcat
     1501 attaataata aaaaatgttt gcaatcaaat cgtacgttgt cgtttgtaat tcttaaaata
     1561 gcaataaata aaatgtttgt tagtaaagta ttattgtgga taataaaata tcgatacaaa
     1621 ttaattgcta taatgcaatt ttagtgtata attccattaa cagagattaa atatatcttt
     1681 aaagggtata tagttaatat aaaatgactt tttaaaaaga gggaataaaa tgaatatgaa
     1741 gaaaaaagaa aaacacgcaa ttcggaaaaa atcgattggc gtggcttcag tgcttgtagg
     1801 tacgttaatc ggttttggac tactcagcag taaagaagca gatgcaagtg aaaatagtgt
     1861 tacgcaatct gatagcgcaa gtaacgaaag caaaagtaat gattcaagta gcgttagtgc
     1921 tgcacctaaa acagacgaca caaacgtgag tgatactaaa acatcgtcaa acactaataa
     1981 tggcgaaacg agtgtggcgc aaaatccagc acaacaggaa acgacacaat catcatcaac
     2041 aaatgcaact acggaagaaa cgccggtaac tggtgaagct actactacga caacgaatca
     2101 agctaataca ccggcaacaa ctcaatcaag caatacaaat gcggaggaat tagtgaatca
     2161 aacaagtaat gaaacgactt ctaatgatac taatacagta tcatctgtaa attcacctca
     2221 aaattctaca aatgcggaaa atgtttcaac aacgcaagat acttcaactg aagcaacacc
     2281 ttcaaacaat gaatcagctc cacagagtac agatgcaagt aataaagatg tagttaatca
     2341 agcggttaat acaagtgcgc ctagaatgag agcatttagt ttagcggcag tagctgcaga
     2401 tgcaccggca gctggcacag atattacgaa tcagttgacg aatgtgacag ttggtattga
     2461 ctctggtacg actgtgtatc cgcaccaagc aggttatgtc aaactgaatt atggtttttc
     2521 agtgcctaat tctgctgtta aaggtgacac attcaaaata actgtaccta aagaattaaa
     2581 cttaaatggt gtaacttcaa ctgctaaagt gccaccaatt atggctggag atcaagtatt
     2641 ggcaaatggt gtaatcgata gtgatggtaa tgttatttat acatttacag actatgtaaa
     2701 tactaaagat gatgtaaaag caactttgac catgcccgct tatattgacc ctgaaaatgt
     2761 taaaaagaca ggtaatgtga cattggctac tggcataggt agtacaacag caaacaaaac
     2821 agtattagta gattatgaaa aatatggtaa gttttataac ttatctatta aaggtacaat
     2881 tgaccaaatc gataaaacaa ataatacgta tcgtcagaca atttatgtca atccaagtgg
     2941 agataacgtt attgcgccgg ttttaacagg taatttaaaa ccaaatacgg atagtaatgc
     3001 attaatagat cagcaaaata caagtattaa agtatataaa gtagataatg cagctgattt
     3061 atctgaaagt tactttgtga atccagaaaa ctttgaggat gtcactaata gtgtgaatat
     3121 tacattccca aatccaaatc aatataaagt agagtttaat acgcctgatg atcaaattac
     3181 aacaccgtat atagtagttg ttaatggtca tattgatccg aatagcaaag gtgatttagc
     3241 tttacgttca actttatatg ggtataactc gaatataatt tggcgctcta tgtcatggga
     3301 caacgaagta gcatttaata acggatcagg ttctggtgac ggtatcgata aaccagttgt
     3361 tcctgaacaa cctgatgagc ctggtgaaat tgaaccaatt ccagaggatt cagattctga
     3421 cccaggttca gattctggca gcgattctaa ttcagatagc ggttcagatt cgggtagtga
     3481 ttctacatca gatagtggtt cagattcagc gagtgattca gattcagcaa gtgattcaga
     3541 ctcagcgagt gattcagatt cagcaagcga ttccgactca gcgagcgatt ccgactcaga
     3601 caatgactcg gattcagata gcgattctga ctcagacagt gactcagatt ccgacagtga
     3661 ctcagattca gatagcgatt ctgactcaga cagtgactca gattcagata gcgattcaga
     3721 ttcagatagc gattcagatt ccgacagtga ttccgactca gacagcgatt ctgactccga
     3781 cagtgattcc gactcagaca gcgattcaga ttccgacagt gattccgact cagatagcga
     3841 ttccgactca gatagcgact cagattcaga cagcgattca gattcagaca gcgattcaga
     3901 ttcagatagc gattcagatt ccgacagtga ctcagattcc gacagtgact cggattcaga
     3961 tagcgattca gattccgaca gtgactcaga ttccgacagt gactcagact cagacagtga
     4021 ttcggattca gcgagtgatt cggattcaga tagtgattcc gactccgaca gtgactcgga
     4081 ttcagatagc gactcagact cggatagcga ctcggattca gatagcgatt cggactcaga
     4141 tagcgattca gaatcagaca gcgattcaga atcagacagc gattcagatt cagacagcga
     4201 ctcagacagt gactcagatt cagatagtga ctcggattca gcgagtgatt cagactcagg
     4261 tagtgactcc gattcatcaa gtgattccga ctcagaaagt gattcaaata gcgattccga
     4321 gtcaggttct aacaataatg tagttccgcc taattcacct aaaaatggta ctaatgcttc
     4381 taataaaaat gaggctaaag atagtaaaga accattacca gatacaggtt ctgaagatga
     4441 agcaaatacg tcactaattt ggggattatt agcatcaata ggttcattac tacttttcag
     4501 aagaaaaaaa gaaaataaag ataagaaata agtaataatg atattaaatt aatcatatga
     4561 ttcatgaaga agccacctta aaaggtggct tttttacttg gattttccaa atatattgtt
     4621 tgaatataat taataattaa ttcatcaaca gttaattatt ttaaaaaggt agatgttata
     4681 taatttggct tggcgaaaaa atagggtgta aggtaggttg ttaattaggg aaaattaagg
     4741 agaaaataca gttgaaaaat aaattgctag ttttatcatt gggagcatta tgtgtatcac
     4801 aaatttggga aagtaatcgt gcgagtgcag tggtttctgg ggagaagaat ccatatgtat
     4861 ctgagtcgtt gaaactgact aataataaaa ataaatctag aacagtagaa gagtataaga
     4921 aaagcttgga tgatttaata tggtcctttc caaacttaga taatgaaaga tttgataatc
     4981 ctgaatataa agaagctatg aaaaaatatc aacagagatt tatggctgaa gatgaggctt
     5041 tgaagaaatt ttttagtgaa gagaaaaaaa taaaaaatgg aaatactgat aatttagatt
     5101 atctaggatt atctcatgaa agatatgaaa gtgtatttaa tactttgaaa aaacaaagtg
     5161 aggagttctt aaaagaaatt gaagatataa aaaaagataa ccctgaattg aaagacttta
     5221 atgaagagga gcaattaaag tgcgacttag aattaaacaa attagaaaat cagatattaa
     5281 tgttaggtaa aacattttat caaaactata gagatgatgt tgaaagttta tatagtaagt
     5341 tagatttaat tatgggatat aaagatgaag aaagagcaaa taaaaaagca gttaacaaaa
     5401 ggatgttaga aaataaaaaa gaagacttag aaaccataat tgatgaattt tttagtgata
     5461 tagataaaac aagacctaat aatattcctg ttttagaaga tgaaaaacaa gaagagaaaa
     5521 atcataaaaa tatggctcaa ttaaaatctg acactgaagc agcaaaaagt gatgaatcaa
     5581 aaagaagcaa gagaagtaaa agaagtttaa atactcaaaa tcacaaacct gcatctcaag
     5641 aagtttctga acaacaaaaa gctgaatatg ataaaagagc agaagaaaga aaagcgagat
     5701 ttttggataa tcaaaaaatt aagaaaacac ctgtagtgtc attagaatat gattttgagc
     5761 ataaacaacg tattgacaac gaaaacgaca agaaacttgt ggtttctgca ccaacaaaga
     5821 aaccaacatc accgactaca tatactgaaa caacgacaca ggtaccaatg cctacagttg
     5881 agcgtcaaac tcagcaacaa attatttata atgcaccaaa acaattggct ggattaaatg
     5941 gtgaaagtca tgatttcaca acaacgcatc aatcaccaac aacttcaaat cacacgcata
     6001 ataatgttgt tgaatttgaa gaaacgtctg ctttacctgg tagaaaatca ggatcactgg
     6061 ttggtataag tcaaattgat tcttctcatc taactgaacg tgagaagcgt gtaattaagc
     6121 gtgaacacgt tagagaagct caaaagttag ttgataatta taaagataca catagttata
     6181 aagaccgaat aaatgcacaa caaaaagtaa atactttaag tgaaggtcat caaaaacgtt
     6241 ttaataaaca aatcaataaa gtatataatg gcaaataatt aatgcatggc tgcaaagcaa
     6301 ataatgagtt tgtcgtaaaa ataacaacat tttaaactag caataaataa tatcaaagtc
     6361 atcatttcaa tgatgcaatc tagtatagtc cacattctaa acaggtgtgg actattactt
     6421 ttttcacttt atattacgaa aaaattatta tgcttaacta tcaatatcaa taattaattt
     6481 taagctgaaa aacaataaaa atgttaagac aacgtttact tcaagttaat tattatactg
     6541 aaaattctgg tatataatgc tgttagtgaa tataacaggg aaattatatt ggttataata
     6601 ttgagtctat ataaaggaga aataacagat gaaaaagaaa ttattagttt taactatgag
     6661 cacgctattt gctacacaaa ttatgaattc aaatcacgct aaagcatcag tgacagagag
     6721 tgttgacaaa aaatttgtag ttccagaatc aggaattaat aaaattattc cagcttacga
     6781 tgaatttaag aattcgccaa aagtaaatgt tagtaattta actgacaata aaaactttgt
     6841 agcttctgaa gataaattga ataagattgc agattcatcg gcagctagta aaattgtaga
     6901 taaaaacttt gtcgtaccag aatcaaagtt aggaaacatt gtgccagagt acaaagaaat
     6961 caataatcgc gtgaatgtag caacaaacaa tccagcttca caacaagttg ataagcattt
     7021 tgttgctaaa ggcccagaag taaatagatt tattacgcaa aacaaagtaa accaccactt
     7081 cattactacg caaacccact acaagaaagt tattacttca tacaaatcaa cacatgtaca
     7141 taaacatgta aatcatgcaa aggattctat taataaacac tttattgtta aaccatcaga
     7201 atcgcctaga tatacacatc catctcaatc tttaattatc aagcatcatt ttgcagttcc
     7261 tggatatcac gcgcataaat ttgttacacc agggcatgct agcattaaaa ttaatcactt
     7321 ttgtgttgtg ccacaaataa atagtttcaa ggtaattcca ccatatggtc acaattcaca
     7381 tcgtatgcat gtaccaagtt tccaaaataa cacaacagca acacatcaaa atgctaaagt
     7441 aaataaagca tatgactata aatacttcta ttcttataaa gtagttaaag gtgtgaagaa
     7501 atatttctca ttttcacaat caaatggtta taaaattggg aaaccatcat taaatatcaa
     7561 aaatgtaaat tatcaatatg ctgttccaag ttatagccct acacactacg ttcctgaatt
     7621 taagggtagc ttaccagcac cacgagtata aaaattggca ctaagtttac gagatatgat
     7681 aaatacctat tattttaaat atagtctaca atctatgtgg ttgtaggctg tattttttgc
     7741 agtttatcaa taaacaccca tcaacaaatt ataccgtttt tctactttga aagttggaag
     7801 taacataatc ttaaataaat atattattaa ttaagataaa tataatactc aagattattg
     7861 ttaatagttt gttcatcgca agttaattat tgtttctaaa atattggtat ataattttca
     7921 atggcgaaga aaacagggtg aaaaagtcgg tttttatatc aaagcaaata agggagcata
     7981 aacaaatgaa aaggaaagta ttagtactaa caatgggtgt aatttgtgca actcaattat
     8041 ggcattctaa tcacgcaaac gcattagtaa cagagagtgg cgcaaatgat actaagcaat
     8101 ttactgaaat tgtatcggaa gagaaagtta taacagttga acatgctcaa attaatattt
     8161 ttcaatctaa tagcaattca aacttgatgg agttcaacat attaacaatg ggcggtaaat
     8221 caggagctat ggttggttat agtgaaattg actcatcaca tttcacagac cgtgacaaac
     8281 gcgttattag acgtgatcat gttaaagaag cacaaagctt agtagagaac tataaagata
     8341 cacaaagtgc tgatgctagg atgaaagcca aacaaaaagt taacacatta agcaaaccgc
     8401 atcaaaacta tttcaataaa caaattgata aggtttataa tggattacaa cgctaatcca
     8461 aagtaaatta taagttatac atctcgtttt taaatgacaa tttatccccg taaatattat
     8521 aaataatctt ttcaaattcc acatagatat agagacacta ataaacctct ttgtctcgat
     8581 atgatagtct gcaacgattc atgttgtagg ctttttaatt ttacaaataa ggctaaatat
     8641 ataagttctg gcacctaaaa tatagaaaat acataaaagt aa
//
LOCUS       DALAAR010000098         8441 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000098 DALAAR010000000
VERSION     DALAAR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.100"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            26..1555
                     /gene="aur"
                     /locus_tag="O2458_002426"
     CDS             26..1555
                     /gene="aur"
                     /locus_tag="O2458_002426"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCZ3888269.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1922..2371
                     /locus_tag="O2458_002427"
     CDS             1922..2371
                     /locus_tag="O2458_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCZ3888270.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2374..2541
                     /locus_tag="O2458_002428"
     CDS             2374..2541
                     /locus_tag="O2458_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888271.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2718..3953
                     /gene="arcA"
                     /locus_tag="O2458_002429"
     CDS             2718..3953
                     /gene="arcA"
                     /locus_tag="O2458_002429"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCZ3888272.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            3986..4996
                     /gene="argF"
                     /locus_tag="O2458_002430"
     CDS             3986..4996
                     /gene="argF"
                     /locus_tag="O2458_002430"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ3888273.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5087..6517
                     /gene="arcD"
                     /locus_tag="O2458_002431"
     CDS             5087..6517
                     /gene="arcD"
                     /locus_tag="O2458_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCZ3888274.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6534..7475
                     /gene="arcC"
                     /locus_tag="O2458_002432"
     CDS             6534..7475
                     /gene="arcC"
                     /locus_tag="O2458_002432"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ3888275.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7574..8278
                     /locus_tag="O2458_002433"
     CDS             7574..8278
                     /locus_tag="O2458_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCZ3888276.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3083 a         1169 c         1640 g         2549 t
ORIGIN      
        1 tttaagattt caggaggaat gaaatgtgag gaaattttca agatatgcat ttacaagtat
       61 ggcagcatta accttgttga gcactttatc accagcagca ttagcgattg attcaaaaaa
      121 taaaccagct aattctgata ttaaatttga ggtgactcaa aagagtgatg cggtcaaagc
      181 attaaaagaa ttgcctaaat ccgaaaatgt aaaaaatatt tatcaagatt acgctgttac
      241 tgatgtaaaa actgataaaa aaggatttac gcattataca ttgcaaccga gtgttgatgg
      301 tgttcatgca cctgacaaag aagtgaaagt acacgcagac aaatcaggaa aagtcgtttt
      361 aatcaatggg gatactgatg cgaagaaagt aaagccaacg aataaagtga cattaagtaa
      421 agatgacgca gccgacaaag catttaaagc agttaagatt gataagaata aagcgaaaaa
      481 tcttaaagat aaagtcatta aagaaaacaa agttgaaatc gatggtgaca gtaataaata
      541 cgtttataat gttgagttaa ttacagtgac accagaaatt tcacattgga aagttaaaat
      601 tgatgctcaa actggcgaaa ttttagaaaa aatgaactta gttaaagaag ctgcagaaac
      661 tggtaaagga aaaggtgtac ttggcgatac aaaagatatc aatatcaata gtattgacgg
      721 tggatttagc ctagaagatt taacgcatca aggtaaatta tcagcattta gctttaatga
      781 tcaaacaggt caagcaacat tgattactaa tgaagatgaa aacttcgtaa aagatgagca
      841 acgtgctggc gtagatgcaa attattacgc taaacaaaca tatgattatt acaaagacac
      901 atttggtcgt gaatcatatg acaaccaagg tagtccaatt gtttcattaa cgcatgttaa
      961 taactacggt ggtcaagata acagaaataa tgccgcatgg atcggtgaca aaatgatcta
     1021 tggtgatggt gatggtcgca cattcacaag tttatcgggt gcaaatgacg tagtagcaca
     1081 cgaattaaca cacggtgtga cacaagagac agcgaactta gaatataagg accagtcagg
     1141 cgctctaaat gaaagctttt cagatgtttt tggatacttt gtagatgacg aggatttctt
     1201 aatgggtgaa gatgtctaca cacctggaaa agagggagac gctttacgca gcatgtcaaa
     1261 cccagaacaa tttggtcaac cagctcatat gaaagactat gtattcactg aaaaagataa
     1321 tggtggcgta catacgaatt ctggaattcc aaataaagca gcttataacg tgattcaagc
     1381 aatagggaaa tctaaatcag aacaaattta ctaccgagca ttaacggaat acttaacaag
     1441 taattcaaac ttcaaagatt gtaaagatgc attataccaa gcggctaaag atttatatga
     1501 cgagcaaaca gctgaacagg tgtatgaagc atggaatgaa gtaggcgtgg agtaaaaata
     1561 tataaacaag aagaagtaat gttaaacact tataaataat taaattttaa atacagctta
     1621 tatatggggt atacctacat tgataattga ctttgtaggt ataccctctc tttgtaatta
     1681 gatgagagga gtgtgagggt tgtctgccga aagactactc ggcagtctaa aatcattaca
     1741 agtagtagat atgtgataat taaatgctga cttagaatac aaaattcatt ttaaaagttg
     1801 tcacaaaaaa tttacatgta tttttattat attttgcaaa acaaagtgtt aaattataaa
     1861 tgaaacatgc atgaatttat tttttaatac aagaaacgta actaccaaag gagtttacaa
     1921 tatgaagaaa agtaaacgat tagaaattgt ttctacaata gttaaaaagc ataagattta
     1981 taaaaaagaa caaatcattt catatattga agaatatttt ggtgtaagat atagtgcaac
     2041 aacaattgct aaagacttga aggaactaaa tatatatcgt gtccctatcg attgtgagac
     2101 atggatttat aaagctatta ataatcaaac agaacaagag atgagagaaa agtttagaca
     2161 ctattgtgaa catgaagttc taagttcaat catcaatggt tcatacatta tcgtcaaaac
     2221 ctcacctggt ttcgcccaag gcataaacta ttttatcgat cagctaaata tagaagagat
     2281 attaggtacg gtgagtggaa atgacactac attaatctta actgcctcaa atgatatggc
     2341 agaatacgta tatgcaaaat tatttaaata gacatgtatc aaatgaataa taaaaatttg
     2401 tttcgtatca cgtgtactca agttagttac caaatattaa cttgtgtacg cgttttttta
     2461 tggaaagaaa gaattcatag tcattcaatt gactgtataa aaaactttat acaacatgtt
     2521 tttatgggtg tttttgaata aaaaatgtat attttgaccc aaaatgcctt tatttatgta
     2581 taaaaatcca ttattatgta ttgtataaca aaaagatatg aaattttcga ctttctttat
     2641 gtgaatataa tcacatgtaa gcgtttgaag attgtctata ctctaaatga attcaaagat
     2701 aaaaggagga aatagacatg acagatggtc caattaaagt aaatagcgaa attggagctt
     2761 taaaaactgt gttacttaag cgtcctggaa aagaattaga aaatttagta cctgattatt
     2821 tagatggatt actatttgat gatattccat atttagaagt agctcaaaaa gagcatgacc
     2881 attttgcgca ggtgctaaga gaagagggtg ttgaagtact ttaccttgag aagttagcag
     2941 ctgaaagtat tgaaaatcct caagtaagaa gtgaatttat tgatgatgta ttagcagagt
     3001 ctaaaaaaac aatattaggt catgaagaag aaattaaggc attatttgcg acactttcta
     3061 atcaagaact tgtagataaa ataatgtcag gggtacgtaa ggaagaaatt aatccgaaat
     3121 gtacacatct agtagagtat atggatgata agtatccatt ctatttagat ccaatgccaa
     3181 acctttattt tactagagat ccacaagcct caataggaca cggtataaca atcaatcgga
     3241 tgttctggag agcacgacga cgagaatcaa tatttattca atatattgta aagcatcatc
     3301 ctagatttaa agatgcgaat attccaatct ggttagatcg agattgccca ttcaatattg
     3361 aaggcggcga tgaacttgtt ttatctaaag atgtcttggc tataggcgtt tcagaacgta
     3421 catctgcaca agctattgaa aagttagcgc gacgtatttt tgaaaatccg caggcgacgt
     3481 ttaaaaaagt agtagcaatt gaaattccaa ctagtcgaac ttttatgcac ttagatacag
     3541 tatttacaat gatagattat gacaaattta caatgcattc agccatttta aaggcagaag
     3601 gcaatatgaa tatatttatt attgaatatg atgacgtaaa taaagatatt gccatcaaac
     3661 aatctagtca tttaaaagat actttagaag acgtactagg tatagatgat atccaattca
     3721 ttccaacagg aaatggtgat gtcattgatg gtgctagaga gcaatggaat gatggctcaa
     3781 atacattatg tataagacca ggcgttgtag tgacttacga tagaaactat gtatcgaatg
     3841 atttattgag acaaaaaggc attaaagtca ttgaaatatc tggtagcgag ttggtacgtg
     3901 gacgtggggg ccctagatgt atgagtcaac cattattcag agaagacatt taacgtaata
     3961 aattatagaa acgagggtga aaataatgac agaaattcaa aaaccgtatg atttaaaagg
     4021 cagatcatta ttaaaagaaa gtgattttac caaagcagaa ttcgaaggac ttattgattt
     4081 tgcaattaca ttaaaagagt ataagaaaaa cggtattaag catcactact tatctggaaa
     4141 aaatattgca ctactattcg aaaagaattc gacgagaacg cgtgctgcgt ttacagttgc
     4201 gtctattgat ttaggtgcgc atccagaatt tttaggaaaa aatgatattc aattaggcaa
     4261 aaaagaatct gtagaggata ctgcgaaagt attaggtaga atgttcgatg gtattgaatt
     4321 ccgtggtttt tcacaacaag ctgttgaaga tttagcgaag ttctctggtg taccggtgtg
     4381 gaatggatta acagacgatt ggcatcctac acaaatgtta gctgatttta tgacaataaa
     4441 agagaatttt ggatatctag aaggaataaa cttaacttac gttggagatg gacgtaataa
     4501 tattgcgcat tcattaatgg tagcaggtgc tatgttaggt gttaatgtaa gaatttgtac
     4561 acctaaatca ttaaatccaa aagaggcata tgttgatatt gcaaaagaaa aagcgagtca
     4621 atatggtggt tcagtcatga ttacggataa tattgcagaa gcagttgaaa atacagatgc
     4681 tatatataca gatgtttggg tatcgatggg tgaagaaagt gaatttgaac aacgtattaa
     4741 tttattaaaa gactatcaag tgaatcaaca gatgtttgat ttaacaggta aagattcaac
     4801 gatattctta cattgtttac cagcattcca tgatacaaat acactttatg gacaagaaat
     4861 ttatgaaaaa tatggattag ctgaaatgga agttacagac caaatcttta gaagtgaaca
     4921 ttcaaaagtg tttgatcaag ctgaaaatag aatgcataca attaaggcag taatggcagc
     4981 aacattgggg agttaatcac taaatggaac gatatgaata tgatgtgtct gatgatataa
     5041 gtgtcatgta cagacacctc atattggtat taaaggagaa atgaatatga acgaatcagg
     5101 agataacaaa ctcagtaaat cttctttaat tggactagtt ataggatcca tgattggtgg
     5161 cggtgcgttc aatataatgt ctgatatggg cggtaaagcc ggtggattag ccattattat
     5221 tggttggatt attacagcta taggaatgat ttcattagcg ttcgtatttc aaaatttaac
     5281 caatgaacgg ccggagctag acggtggtat ttatagttat gctcaagcag gatttggcga
     5341 ttttgtagga tttatcagtg cttggggata ttggttctca gcgtttttag gcaatgttgc
     5401 ctatgcaaca ctattgatgt cagcagtagg taactttttc ccgattttta aaggaggcaa
     5461 cacattacca agtgttattg tcgcctcgtt actactctgg ggtgtccatt tcttgatttt
     5521 aaaaggcgtt gaaacagcag catttatcaa tagtattgtt actgttgcaa agttaatacc
     5581 gattttactt gtaatcatat gcatgataat tgcattcaat tttgacactt ttaaaacagg
     5641 ctttttcagt atgacgtcag agggtgtatt gccatttagt tgggcgagca caatgagcca
     5701 agttaaaagt acgatgctag tgacagtttg ggtgtttatc ggtatcgaag gtgcagtaat
     5761 tttttctagt agagctaaaa ataaaaaaga tgtaggtagt gccacggtta taggacttat
     5821 atcagtttta attatctatt tcttattaac tgtattagct caaggcgtga ttttgcaaaa
     5881 tcatatttcg caattagatt cgccaagtat ggcacaggtg cttgcaacta ttgtaggtgg
     5941 ttggggatct acacttgtaa atattggttt aattatttcg gtactaggtg catggttagg
     6001 atggacactg cttgctggtg aattaccttt cattgttgca aaagatggat tatttccaaa
     6061 atggtttgct aaagaaaata aaaatggagc acctgtaaat gcactgctta ttaccaatat
     6121 attagtacaa ttatttttaa taagtatgct atttacacag agtgcgtatc aatttgcatt
     6181 ttcactagca tcaagtgcta ttttataccc ttacatgttc agtgcatttt accaagttaa
     6241 atacacttta gagcatcgac agcaagcaac tactaaacaa tggacgattg gtatcatagc
     6301 ctcaatttat gctatatggc ttatatatgc agcaggtatc aattacttat tattgacgat
     6361 gttactttat attccagctc ttcttgttta tacaatcgtt caaaagaata atcagacacg
     6421 tttgattaaa tcagactata ttctttttat gattattatc gtacttgcag ttatcgggtt
     6481 aattaagtta ttgatgggaa cgataaatgt tttttaaaag gagcgacaaa aatatgaaag
     6541 agaaaattgt cattgcatta ggcggtaatg cgatacagac aacagaagca acagctgaag
     6601 cacaacaaac agctattaga tgtgcgatgc aaaaccttaa acctttattt gattcaccag
     6661 cgcgtattgt catttcacat ggtaatggtc cacaaattgg aagtttatta atccaacaag
     6721 ctaaatcgaa cagtgacaca acgccggcaa tgccattgga tacttgtggt gcaatgtcac
     6781 agggtatgat aggctattgg ttggaaactg aaatcaatcg cattttaact gaaatgaata
     6841 gtgatagaac tgtaggcaca atcgttacac gtgtggaagt agataaagat gatccacgat
     6901 ttgataaccc aactaaacca attggtcctt tttatacgaa agaagaagtt gaagaattac
     6961 aaaaagaaca gccagactca gtctttaaag aagatgcagg acgtggttat agaaaagtag
     7021 ttgcgtcacc actacctcaa tctatactag aacaccagtt aattcgaact ttagcagacg
     7081 gtaaaaatat tgtcattgca tgcggtggtg gcggtattcc agttataaaa aaagaaaata
     7141 cctatgaagg tgttgaagcg gttatagata aagattttgc tagtgagaaa ttagcaacgc
     7201 tgattgaagc agatacctta atgattctta cgaatgtaga aaatgtattt attaacttta
     7261 atgaacctaa tcaacaacaa atcgatgata ttgatgtagc aacactgaaa aaatacgcgg
     7321 cacaaggtaa gtttgtggaa ggatcgatgt tgccaaaaat agaagctgcg atacgatttg
     7381 ttgaaagtgg ggaaaacaaa aaagttatca ttaccaattt agagcaggca tacgaagctt
     7441 tgattggtaa taaaggtaca cacattcaca tgtagcactt tatcacgcga caaaacatta
     7501 aatatgtttc tccgttgatt caaatgaaaa agttgtctgc tgacactttg caaggtttga
     7561 aggagtttaa cttatgacag aaaactttat tttgggtaga aataataaat tagaacatga
     7621 actaaaggca ttagcagatt acattaatat accatatagt atattacaac catatcaaag
     7681 tgaatgtttt gtcagacatt atacgaaagg ccaagttatt tatttttcgc cacaagaaag
     7741 tagcaatatt tactttttaa ttgaaggtaa cattattaga gaacattaca atcaaaatgg
     7801 agatgtatat cgttatttta ataaagagca agtattattt ccaatcagta acttatttca
     7861 tccgaaagag gttaacgaat tgtgtacagc attaaccgat tgtacagttc ttggattgcc
     7921 tagagaattg atggcctttt tgtgcaaagc taatgatgat atatttttga cactttttgc
     7981 attaataaat gataatgagc agcaacacat gaactataac atggcattaa caagtaaatt
     8041 tgctaaagat cgaattatca aattgatatg ccatctatgt cagacagtag gatacgatca
     8101 agatgaattt tatgaaatca aacagttttt aactattcaa ctcatgagtg atatggctgg
     8161 gatttcccgg gaaacagctg gtcatattat tcatgaactt aaagatgaaa aacttgttgt
     8221 taaagatcat aaaaattggt tagtaagcaa acatttattc aatgatgtat gtgtttaata
     8281 tacaatgtaa aatgaataag ttgaacatga ggtctaacgt acatttatac gttaggcctt
     8341 ttttgctagc atgatgaata atttaaaatg ttagttaaat ttgattgttg aaattacagt
     8401 aaaatttaag gtgatgaaaa atttagaact tctaagtttt t
//
LOCUS       DALAAR010000099         8391 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000099 DALAAR010000000
VERSION     DALAAR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.101"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            39..1562
                     /locus_tag="O2458_002434"
     CDS             39..1562
                     /locus_tag="O2458_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888277.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1630..2796
                     /locus_tag="O2458_002435"
     CDS             1630..2796
                     /locus_tag="O2458_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888278.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2793..3713
                     /locus_tag="O2458_002436"
     CDS             2793..3713
                     /locus_tag="O2458_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888279.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3727..4386
                     /locus_tag="O2458_002437"
     CDS             3727..4386
                     /locus_tag="O2458_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888280.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4438..5388)
                     /gene="lytM"
                     /locus_tag="O2458_002438"
     CDS             complement(4438..5388)
                     /gene="lytM"
                     /locus_tag="O2458_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCZ3888281.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5714..6706)
                     /locus_tag="O2458_002439"
     CDS             complement(5714..6706)
                     /locus_tag="O2458_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ3888282.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6940..8316
                     /locus_tag="O2458_002440"
     CDS             6940..8316
                     /locus_tag="O2458_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3888283.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2815 a         1429 c         1356 g         2791 t
ORIGIN      
        1 tgatatgatt aactcataaa taattgggag gaagcaaaat gtctaaaaag ttaaaaatta
       61 taattcctat tattattgtc ttactattaa taggtggaat cgcatgggga gtttatgcat
      121 tttttgctaa tacaccgaaa aatacatacc taaaaagtga acaacaaaca gcaaaaatgt
      181 ataaagatta ttttaatgac cgttttgaaa atgaagtgaa gttccaagaa aagatgaaag
      241 ataattcgtt tttatcttca ttagaattaa gcgcagatgc atcagatgaa attgttaaag
      301 ggcttggcat tcctaaatct gttgttaatg catcgaaaat taaaatgtca tatggacatg
      361 atcctaaaaa agagaaatca atgattaatc ttgaaccaac aatcgcagac tctgcattag
      421 ggaaattcca gttagctgca gataaagata agcattattt cgaatcacca ttatttaaag
      481 ggaaatatag tgttaataat tctgatttat tatcaactta ttcaaaactt acaggtgaag
      541 atgaagaaac agcaaaagaa aatggtatta caaaccaaca actaaattta aatactcttt
      601 ttaataatgc tcaagcacaa caaagtgact acagcaaaat tgccgaaaaa tattccgaac
      661 ttattgtcga caaattagat gacgataatt ttgataaagg taaaaaagaa gaaattaagg
      721 ttaatggtga aaagtacaaa gttagacctg tcacgttaac acttagcaga gctgacacta
      781 aaaaaattac attagctgta ttagaagaag ctaaaaagga taaagacctt aaaaaattaa
      841 tggaagaaca aggtgctaca aaagactttg aaaaagacat caaaaaagca attgacgatg
      901 tcaaagaaac taaaaaggat gaatttgcta aaattcaatc taaaatttat accgaaaaac
      961 atacgattgt aaaacgagaa attactatta cagacaaaga aaataataaa actaaaatca
     1021 aaggtactaa tactttagaa gacgataagt taaaactaga ttacgcactt gatttcgatc
     1081 aagataaata cacgtatgct gaagcgaaat atacaattaa aggcgtatct tctaaagaaa
     1141 aagataataa atataatgat aaatacgaat ttggtaaaaa gacagaatat gatgaatcaa
     1201 aaatcaaatt agataaccaa gaaaaagtag atggcacaaa acgtcaagat aaaggtaaaa
     1261 tcactgtcgc attagataaa tatagcgacg aaaatgaatt cacttttgaa aataatatag
     1321 attctgacgt aaaaaataac actcagaaat ctacgttaaa tatcggcatc aaatatgctg
     1381 aagaaccaat taatttcatt ttaaaatcta gcacaaaatt gaaagcagat attgattttg
     1441 atgatagtgg tgcaaaagat ttcaatagtc tatcttcaaa agaccgtgaa aaacttgaaa
     1501 aagaaatcga aaaaaatggc ggcaaaatgt ttgaatcaat tttaaaaaag gcatctaaat
     1561 aactaaaata taaaaataag attggaaggt catcttgcct ttcaatctta tcattggagg
     1621 agtggttaaa tgaaaacaat acatttgttt cgcatctacc actctttttt attgaaaaag
     1681 tggtatttga ttatctattt actatttata ttagcggcac tccttattac attaacgaca
     1741 atccaacatg taacagaaga tgacaatcat tttaatatag gtgtcgtaga taaagatcaa
     1801 tcaagtgaaa cgaaattaat cttaaactct attggtaaag ggagtaacct aggaaaaaac
     1861 gtgagcatta aagcatatga tgataagcaa gcacatactt tgttaaaaaa acataaactt
     1921 caaggctatt ttgtttttga taaaggtatg accaaggcat tttataaaca aggcgaacta
     1981 ccaatttcag tatatacata tgatcaacaa tccatgaaaa gtgtcgtgct atctcagcta
     2041 acagattctg tttaccaacg tcttatgcga tcaatgggtg gcatcttagc ttttcaagac
     2101 ttagcaccga aagcatcaca ttctgacagt atcaatgtta tgactgattt gctgattaca
     2161 ggattaaacc gttcaggtgc atttaactta gaaccgattc atttatacga tacgggcagt
     2221 tattatgcaa ttacaggatt tttaacaacg gtattcatct ttgcactatc tttatttaca
     2281 gttttgaaaa tgaatcaaga tactgtattg aaagcgcgat tgaaaatgtt tcatttttct
     2341 aaagagcgtt tattaatcat tcgtacgttg attacatggt tttatactat gttatggagt
     2401 atcgttggtg tagtttggat tgtgttcagt attccgaata tctttgaatt atataattgg
     2461 ccaacgttag ccattcattt aagctattat gttacctttt taatactgtg gttattactc
     2521 atcgaacttt taacaacagg cttgttgaat agtattagta aagtcatatt agctattgtc
     2581 atcctggtgt tatctggttt aacaatacct acgatttttt tacaacatat agcaaatggt
     2641 gttttcaata ttcaaccgtt cgcagttgta acaaatcaat tgttagaaat tattttaaac
     2701 aactacattt tagaactgca ccctagtttc tatcttagct tcatcgcact tttgataatt
     2761 aatttagctg tattggtttg gaggtatcgt caatgaagcc ttatatacaa cttgttgtgt
     2821 tcaagcaatg gttacaatac atcttgctcg taacaaccat tgtcatcgca ctcgtactta
     2881 ttggtatcgg ttaccgtgta gcacatgaca acttcaaaat accgattacc attcaagatt
     2941 tagaccaaac cactgcatca aaatcattcg tcaataaaat taaacaatct gactatgtaa
     3001 ctattaaaaa agtcgatgaa gatgaaagct atattgaaga tgatgttact aaaaaggaag
     3061 ctattttaag tatgcaaatt cctaaaggtt tctctcaaaa attaaaagag aaccgtttaa
     3121 aagaaacgat acagttatat ggtagagatg actttatagg tggtattgct gtagaaattg
     3181 ttagtagttc attatatgag cagcaaattc ctaacattat ttatgaacac cttgaggata
     3241 tgaaacagca tcaatccatc gatgctatca acaagtccta tcataaacat acacctgaat
     3301 ctaaaatcaa atttgtgtcg cttactaaac aagcacaaca ctctatttca attagcttaa
     3361 tctttgcggt gattttattt gttagcgctg ttcaagtagt acttcattat cgtttaaacc
     3421 aacaagcagc attgcaacga ttatcacaat atcatttaag ccgtttcaaa ctatatagta
     3481 cttatgtaat gacacatacg attttgttat tgttggtact attggcagtt agtctatatt
     3541 tgtctcaacc actcagctta atattttact taaaatcact gttacttata ttgatttatg
     3601 agataggtat cgtctttatc ttattccata ttcaaacaat aagtcatcga ttattcatga
     3661 catttatata tgcacttgct atgggtatcg tatacttgat tattttcatg taaaggagcg
     3721 taactgatga tagaaattaa taacctttca aagcgttacc gtaacaaaca gattttcaat
     3781 catttaacta tgtcctttga tagtaatcgt ttaaccgtat tacttggtga taatggtgct
     3841 ggaaaatcaa cattacttcg tatgattgct ggtattgaaa aagctaatga tggaactatc
     3901 aactatttcg gcgaaaaatg gaatcaaaga caaatacaaa atcacatcgg ttatgtgcca
     3961 caagacattg cgttatttga acacatgaca gtggctgaaa acattaaatt ttttaaatca
     4021 ctttgtaaaa atccaattaa cgatacaact atcaacgaat atttacagca attaaacttt
     4081 gatgatacgt ctgccaaagt atctacattg tccggtggga ataaacgtaa aattaatata
     4141 ttagtaggtt tactaggtca acctcgaatt ctcattttag atgaaccgac agttggtatt
     4201 gatttaaaat ctagacatga catccaccaa ctacttaaca tcatgaaatc taaatgttta
     4261 attatattaa ctacccatca tttagatgaa gttgaagcac ttgcagatga tatcaagtta
     4321 attggccaag atccttttta tcaacatgtt ttagaggaca aacaatggac ttatacctat
     4381 tattaaaacg aaaaaatccc aagctgcgta tgatatcgca acttgggatt ttctgtatta
     4441 tctactttgc aagtatgacg ttgggtctac tgcatattga ttaccgatgc caccagacat
     4501 acgttggaag tgtacgtgag gcgctgttga attacccgta ctacctgaat atgcaatttg
     4561 gtcaccagct ttgactttat caccagctga aacagttaaa cgattattat gcatatacca
     4621 ttggtagtta ttactgttcg cttctttaat cgttacttga ttgccgccac catagttact
     4681 ccaacctgct tgtactactg taccatcagt taatgagtaa actggtgaat tttcaggcat
     4741 tgcatagtcg acaccgtaat gcgcaccacc accgtgatat tgtccatatg gttgtagttg
     4801 tttacgactt gttaaccagc ttgcgtcttt cgcatgacca ctagctgtcg ctttacttgc
     4861 tgatccacca ttttgattag atgtgccatt aggataattg acctttccat taccatcatg
     4921 gctgttatac gcttggttgt tgttactatg tgaataatag ctcgcgtctg gacctacatt
     4981 tgattgataa ccatattgat taatatgctg ttggctttga ctcgctgtgt agtcattgtt
     5041 atctcctgct gttgctggat tcacatatgt ttggccgctt ccattggcat ttgcattttt
     5101 tggataacag ttataaaaat aatgcgtatg tccttgagca tctacgaatg tatagctata
     5161 ttctttatta tcaaacattg cttgattcca gttaccatca ggtgtgtgat gataatcccc
     5221 attagaatca attgtataat aagtaccata agatacgtct tgtgattgtg ttgacatttg
     5281 tgtatgtgct tgttgggtgt ttgtcgtttc tgctgcatct gcttgatgcg ccattgtaaa
     5341 tgtagcgaag cccatcgttg caatcgctgc tgctgttaat tttttcatgt ataaaacatc
     5401 ctccattaaa gttaaagtta gttttcaatt aaactgtact gcacatacta aaagaattag
     5461 acaactgagt aaaggattta attctcattt tccaactatt taatattccc gaaatgtttt
     5521 actaaactca ttacattgtc attacaaaat agccatacat tgatattaaa atgacatctc
     5581 tcactgcatt cgtttaacct tttataaatt ttcaaaatta acaactaatc gttcgtccat
     5641 gttcgcgatt caacgctaat gcatgatagt aatcatccat caaatcatat caaccaaatt
     5701 ccattatcaa tcgctattga ttgtcattca actttctaat agtgatatgc ttctcaggct
     5761 taaaaatcgt catatctttt ctattaatta aatcatctgt gagctttaat gctactaatt
     5821 cattgctgcc ataatactta atatataacg ttcttgtagt taaatttatt acggtctgat
     5881 acatcgtata gtgattgtca gcatcatgcg gacgtacaat tccaatcggt atatttaccg
     5941 catctaataa ataaaatgca ttcattaaat ccatttcttt atcattgttt tgagcaatgt
     6001 ttgctttcat aaatgccatt ctcacaaagc gctcagttga agtaaatcca cctggcaatc
     6061 caaatgtacc tgcttcattg cctaaaggtt caatcgttac accttccaat aaatttgctg
     6121 ttgctggata aggagaaata ttgatatatt gtcttaaatt actataatgc caatttaagt
     6181 ctggatgatt tgttaagaca ccaataggat tatcttttat aaccacttca ccctctttaa
     6241 atgaaacttc gactgtatgt ccagttgcat cggaaacatg ataatgcaat ggcggaactt
     6301 caccgatgtc atttaaatat acagctacaa catgtatttg ggatgcttgt tgtttcatat
     6361 cttcaatgct tgttgtatat cccaaaatcc atgtcacaat ttcattttgc gtaatattca
     6421 tcgcgtccgc tttgtgtgtt gatccatatg aactataacc tcggaaatat tgtgttgaaa
     6481 tggcaacgcc atgttcatta acaccatcac cataaataaa accttccata tctgttcctg
     6541 tgccaataaa gccatattgc gtttggcctg tcgtgccagt gcgagatttc caacgataat
     6601 ttctaggcgt cactgctggc gaaccatcta atggataatc ataatccatc gtgcgtccaa
     6661 gaagtacttg attatttaaa gtttgtattg tgaatcctgt gcacattgtt ctcactcctc
     6721 tgtaccttca tttactttaa tcactttcaa ataaagctgt ttcacttaaa catactataa
     6781 aaaatcaatt atacaagcaa ttaattgata ttcattctca ataactgtgg tatgatatgt
     6841 aaggaaatca tgacttatgt gtgagtgaac gatcatctat acatccgttc acttcatctc
     6901 atgactttct atatttaatt tttacaagga gtgacatctg tgaataacac acaatcttca
     6961 ccacgcagta atattattat tgcgattatg ttgtctgcat taacatattg gttgtttgca
     7021 caatcattta ttaatatagg acctctcgtt ggtcaaacgt atcaaacctc tcctgccgtg
     7081 ttaaatttat ctattagttt aacttccttc gccacaggta tcttcatggt ggctgcaggt
     7141 gatattgctg ataaaatagg acaactgaga atgacataca tgggtctcat aatcagtatg
     7201 tttgcatctc ttctattaat tatatcggac atcactgcac tgctcatcat cggtagaatt
     7261 ttacaaggtc tatcagcagc tatcttgtta ccttcaacag ttggcgtgtt aaataatcaa
     7321 tttaaaggag aacatttaag acgagcgatt agttatctaa tgattagtac tgttggtggc
     7381 atcggcctag ctggtgttat cggcggttta attgcctcaa atttcggatg gcaaacgaat
     7441 ttcatcatta gtatagtcat tgctttcatt gccatattgc ttctaaaagg cacacctgaa
     7501 aaagtaagtc aacatagcca ccgtcatcca ttcgattaca aaggtatgtc gattttcgct
     7561 gttatgattg gtagctttac attattgtta acacaaggat tcgaacaagg ttggtttagt
     7621 acattttcaa tcatttgtct gagcattttt atcatcacta cgttgatatt catcatcatc
     7681 gaacgtcgac atgaagtacc ttttattgat ttctcagtat tacgcaaccg tccgttcatt
     7741 ggtgcatttt taaataactt tgttttaaat agcggtctag gcgtaacagt ggtctttttc
     7801 atatatgctc aaacacacct tggtttatca gctgcgcaat ctggacttgt tacattgcca
     7861 tatgccattg tggcagttgc gatgattcgt ttaggtgaaa aagcaacatt acgtttcggt
     7921 ggcaaattga tgctcatcat tggtccgttg ttccctgtca tcggcatcac tattattagc
     7981 atgactcaat tattggcatc acaatatgtc attgcagtta tcattggttt cgtcatatgt
     8041 gcgataggta atggtttagt cgcaacacct ggacttacga ttgcaatttt cagtatgcct
     8101 aatgaaaaag ttggtttagc tacaggatta tataaaatga gtggtacatt aggtggcgcc
     8161 tttggtatag cactaagtac tacagttttc agtatgttac aactaaacta tgcaccaagt
     8221 gtagctgcaa ccgtaacatt tatagtcagc attgtattga tgatccttgg ctcattgtct
     8281 gcatacatga tcattccaaa aacagttaaa tcttaaatat aatagaagaa ttatgtttcg
     8341 aaatatcttt atcactttaa aatgatatac aagaaatcca agaaaaataa g
//
LOCUS       DALAAR010000100         8374 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000100 DALAAR010000000
VERSION     DALAAR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.102"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..274)
                     /locus_tag="O2458_002441"
     CDS             complement(<1..274)
                     /locus_tag="O2458_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCZ3888284.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYR"
     gene            536..721
                     /locus_tag="O2458_002442"
     CDS             536..721
                     /locus_tag="O2458_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888285.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(944..1522)
                     /locus_tag="O2458_002443"
     CDS             complement(944..1522)
                     /locus_tag="O2458_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888286.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1786..2166)
                     /gene="blaI"
                     /locus_tag="O2458_002444"
     CDS             complement(1786..2166)
                     /gene="blaI"
                     /locus_tag="O2458_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ3888287.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2156..3913)
                     /gene="blaR1"
                     /locus_tag="O2458_002445"
     CDS             complement(2156..3913)
                     /gene="blaR1"
                     /locus_tag="O2458_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ3888288.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4020..4865
                     /gene="blaZ"
                     /locus_tag="O2458_002446"
     CDS             4020..4865
                     /gene="blaZ"
                     /locus_tag="O2458_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ3888289.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5391..5810)
                     /locus_tag="O2458_002447"
     CDS             complement(5391..5810)
                     /locus_tag="O2458_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3888290.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5934..6905
                     /locus_tag="O2458_002448"
     CDS             5934..6905
                     /locus_tag="O2458_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ3888291.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7077..7169)
                     /locus_tag="O2458_002449"
     CDS             complement(7077..7169)
                     /locus_tag="O2458_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ3888292.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7326..7673)
                     /locus_tag="O2458_002450"
     CDS             complement(7326..7673)
                     /locus_tag="O2458_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888293.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7692..8309)
                     /gene="cadD"
                     /locus_tag="O2458_002451"
     CDS             complement(7692..8309)
                     /gene="cadD"
                     /locus_tag="O2458_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCZ3888294.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2970 a         1223 c         1097 g         3084 t
ORIGIN      
        1 cacgatataa atagctccat tttcctttta ttttgatgta cgtctcatca atacgccatt
       61 tgtaataagc ttttttatgc tttttcttcc aaatttgata taaaattggg gcatattctt
      121 gaacccaacg gtagaccgtt gaatgatgaa cgtttacacc acgttccctt aatatttcag
      181 atatatcacg ataactcaat gcatatctta gatagtagcc aacagctaca gtgataacat
      241 ccttgttaaa ttgtttatat ctgaaatagt tcatacagaa gactcctttt tgttaaaatt
      301 atactataaa ttcaactttg caacagaacc tcatttacta tatgggcatc tctttcatta
      361 ttaatacaaa gctttgtatc tccgatatta ggcaaaggtg taaacgaatt aggtgatact
      421 ataggattca acattatatt aatcgtttct ttaataatga ttatcttctt attttttata
      481 aatcgtagta aaaatatctc ctatgattat ataaaagttc aaagaggtga agccaatgaa
      541 taaattagaa aaatggtctt tggtatttat tatttttaca tttctctctt cattagcact
      601 tttatatttc aaatataatt ttagttcggt tatgccagtc ctaagtcttt ctattttaac
      661 tatactaata ttgttgacga gtattattac tgtagtttca gttaatgatg aagatactta
      721 aaacgttcat tagtgaaaca ggtattatat taaaaaagcg tacgaatttg tacgcttttt
      781 tcttgtgtta ttattaaaat ctcataacag gaaaatatta tgagatttta gacgctgtca
      841 gaacgtctct tttacatcga gtgacattga atcaaagtct ttgtatgtta atgatatata
      901 aaattattta gtagattcat tatagtaagc tagattagtt gctctacttg tttttattct
      961 cttttgcctc taatttattg agataacgat aaatagttgt acgtgaaact tgccactgct
     1021 ctgcaattgt tttaattggc gtaccattat cataaagtgt cttaagtaag tttaaatctt
     1081 gtttatttaa tttttcaggt ctacctccat atcggcctct agcacgtgct gcaattcgac
     1141 cggctgatga tcgctctaaa attaaattac gttcaaactc tgcaaatgct gcgaataaat
     1201 gaaataataa ttgtcctgta gaacttgatt tatccatcgt tatattttct tctaaactat
     1261 gaaaactcac acctcgttcg ttaagttcat taactaacgt gattaaatcc tccatgttcc
     1321 gtcctagtct atctaatctc caaacaacaa tcgtatctcc tgacctcgca aattcaattg
     1381 ctttatctaa accaggtcgc ttactttttg aaccactcat atggtcatta aatatctttt
     1441 cacaaccata tgtatttaat cgatcctcct gtaaattcaa attttgtaat ccagttgaaa
     1501 ctcttgcata acctattttc aaaatacaga cctcctatat acacctttag tgtaccataa
     1561 cttgattacg aggtcttttg tgaacataga ttttttgttg ggtttttgaa cacataattc
     1621 tatgtaatac ttacttattt agacaaaaaa caagagtgta cagaaaatgg ttattttctg
     1681 gacactctca tctttaataa tatattttaa atgtcattaa caaaactaat ttaataagag
     1741 tcaagtatgg tttacatatg tattatgttt gaaacatacc tctaattact ttttactaat
     1801 atcatttaaa atgtctcgca attcttcaat ttctttgtta tttaattctt catttttcgc
     1861 aaaattcagc actaaacttt tcatatcccc tccatacagt ttattaagaa aggttttagc
     1921 agttttcatt ttaatatcgt cttctttaat atttgatgag taaaaataaa tattttctga
     1981 tttgtatcgt tttataatct cttttttata tagtcttgtg attaatgttc taatcgtttt
     2041 atcactaact tctttatatt tttgaatttc aactacaatt tcattagctg atactgattt
     2101 tttatcccat attatattca taacatccca ttcagccata gatatttcaa cttgcttatt
     2161 ggtcatttaa aacacccatt ccttttaata ttttttcact tattaattca gcatttttcc
     2221 cagacgggtt tccatctgat aaatgtgtag caaaataata cttatcatga tttgtaatta
     2281 cgtaacctac aaaccaccca ttattatact tcccgtttac tatacctgta cctgttttcc
     2341 catacagttc atacttttca tttttcttaa tcaataatga agaagataat tgattctttg
     2401 cttttttact aaaatggtta ttttgttcca tcatattttt aaaaactatt acttgttcaa
     2461 gattagatat tttcaaacta tcttccatcc aatagctttt ataacttccc aaattttcat
     2521 taccataatt taattgcttg agttgagccg cagtatagtt ctttggtatt tgattactaa
     2581 tacgttcgaa gtaccaatta actgaatttt gcattgctgt atttaaatct tgttccttat
     2641 tccaagactc gaaaagataa tgtttatgat tccatgacat acgtgaattt ttatcactta
     2701 taatatgacg gtcaagtcca aacagtgcta aataaatttt gtaagttgaa tcaggagaat
     2761 atctttttct actttctttt tcattataaa tatagtattt gtctttcttc atgctgtaca
     2821 taacgaaaga tccactattt gaaccaaaat ttttactttc atctaaaatt tggtaatcgc
     2881 tttgtaaggg ctttttataa ttataatcag ttaaggattg ccccattaaa aactggcttt
     2941 gaattatcat gataaaaaaa gtaaaaatac aaataaaaat tagaattaat tttgactgct
     3001 ttttcagatc ggcttctttt atattaatta atctactttt gagtaatgat tttttaccat
     3061 taaatgaatg gcttaatata tttttgttgg aaaatggggt ttttaatacg gagtccataa
     3121 tagattccgc ataagtttta aattcatttt tgttaatatt attaaggaca aatctatcgg
     3181 cttctacttc attgtcatga attatctttc tttttacaat atgtactaat ggattgtaac
     3241 tcataataat agagaagata ttaaagataa ttaaatgtaa agtatctcta tttttagcat
     3301 gagcatattc atgcagtatt atatatttta gtcttttatc aattacactt ttaaaatatg
     3361 aactaggaat caaaataata tatttcccat accaaaaagt tattggagat tgaatagcct
     3421 ctgcttttcg aatcacaata ttttttttat attgatggtt gaaaagtatc gtatctattt
     3481 tatttttttc attttcgttt agataaagtg actgtttctt taaatattta agatataata
     3541 aggctttcaa aaatttaaaa cttaaaataa taactaaaac tatccaaata actgtgcaga
     3601 tattatcaat tgaatcccaa ttaaacttat ggatatctgt tgtgaactct tgaataggtt
     3661 tggtggtatt tatgttatgg ttcaaatcgt gtgacttact ttcaactgtg ggcgcttgat
     3721 tattcacatt attaaatttt ataaaagaga atttaattgg gataaaagga attaatccag
     3781 caagcatagt tagataccaa actttataat ttaaactata attaaaatag cgttttaata
     3841 tgtacctaaa aaatactaac aatagaaata taaaacaaaa gcttactacg ctcattatta
     3901 gtaatttagc cattttgaca ccttctttca aatatttata ataaacaatt gacatcaata
     3961 ttacaattgt aatattattg atttataaaa attacaactg taatatcgga gggtttattt
     4021 tgaaaaagtt aatattttta attgtaattg ctttagtttt aagtgcatgt aattcaaaca
     4081 gttcacatgc caaagagtta aatgatttag aaaaaaaata taatgctcat attggtgttt
     4141 atgctttaaa tactaaaagt ggtaaggaag taaaatttaa ttcagataag agatttgcct
     4201 atgcttcgac ttcaaaagcg ataaatagtg ctattttgtt agaacaagta ccttataata
     4261 agttaaataa aaaagtacat attaacaaag atgatatagt tgcttattct cctattttag
     4321 aaaaatatgt aggaaaagat atcactttaa aagaacttat tgaggcttca atgacatata
     4381 gtgataatac agcaaacaat aaaattataa aagaaatcgg tggaatcaaa aaagttaaac
     4441 aacgtctaaa agaactagga gataaagtaa caaatccagt tagatatgag atagaattaa
     4501 attactattc accaaagagc aaaaaagata cttcaacgcc tgctgctttc ggcaagactt
     4561 taaataaact tatcgcaaat ggaaaattaa gcaaaaaaaa taaaaatttc ttacttgatt
     4621 taatgtttaa taataaaaac ggagacactt taattaaaga tggtgttcca aaagactata
     4681 aggttgctga taaaagtggt caagcaataa catatgcttc tagaaatgat gttgcttttg
     4741 tttatcctaa gggccaatct gaacctattg ttttagtcat ttttacgaat aaagacaata
     4801 aaagtgataa gccaaatgat aagttgataa gtgaaaccgc caagagtgta atgaaggaat
     4861 tttaatattc taaatgcata ataaatactg ataacatctt atagtttgta ttatattttg
     4921 tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt attattttcg
     4981 agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca aaaaatcata
     5041 aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat
     5101 ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa
     5161 gtgacaatag cattttacaa gtatttttta ttgttcgatt agggtaaacc taaataatac
     5221 ataaattata ttacaatatg tttattatag aggctaaagt atcttagaaa tttggatagt
     5281 aggtttttcg ggaataaaag tatagttggc tttaatatat aattaagcta agctatcatt
     5341 tatatttact cgattataat tcgcacttca acttttttat aacgtaaaaa ttaaaacgta
     5401 gttttattta tctttagtaa tatcttttta aacactacag tttcttcatt agttaaatta
     5461 gcgaagtaat gattattaag ttgataggct ttcttttcta atgttggcca taagtctttt
     5521 gctttatttg tactgtttat tagcacagtt cttctatttt cattatcagt ttgacgtgtt
     5581 attagatcct ttttggacat cttatctaat attccagata ttgttgaagg ttctaaattt
     5641 aaagtattac caatcgcttt aggtgtattc aaattactat taatcaagca attcaaaaca
     5701 ccatattctg ctggagtaat attataggga cttaataaac ttttaaaatg tttaaaaact
     5761 ctattctgag aagtactcaa caaataattt atacaattat ctaattccac aactaaaacc
     5821 tctcttaaat cattagtatt gactcgctta tcatatcatg ataagttttg taggaaaata
     5881 gttttgcaac aaaaacatac ttctacaaaa caattgtgaa agaaggctaa taaatgttca
     5941 atgcggtagt agtgagaaaa agtgcatcaa atgttagtta taaagtagaa aaagttgatg
     6001 aatctcattt atcgaagggt gatgtattag taaaagtggt atactcttca ataaattata
     6061 aggatatgct agctttacaa tataaaggcg gtgtaattag agattatcct atgattcctg
     6121 gtatagattt tgcaggtatt gttgaatcaa gttcgaatga taagtttaaa gaaggcgata
     6181 atgtattagt tactggttat gatgttggcg tgacacatac aggtggattt tcagaatatg
     6241 cacaagtgcc tggagagtgg attgttccta ttccccaaga aatgtctatt aaagagtcta
     6301 tggtatatgg cacagccggt tttacggcag cattatcaat ttttgaacta gaaaaatatg
     6361 gaatgaatat caaatcacaa cctgaaattt tagtgtctgg ggctacaggt ggtgtgggca
     6421 gtgttgctat acaaatttta cataaaattg gatatgaaaa tatcactgct atcgtaagaa
     6481 aagaaaagca aatagaaatc gcaaaaaaat taggtgcaaa acaagtaata ataattgaag
     6541 atgataataa accgcttaaa aaaggtattt ttgattattt cttagataca gtggggagta
     6601 atgtaacatt atacggttta aaacgtctaa attatcaagg tgttgctact gtatgtggaa
     6661 atgtagcagg aaattcactt aacgctaata tattaccgtt tattcttaga ggtattaaat
     6721 taattggcat tgattctgta tatgttgatc ataatatccg tgaagatata tggagtaagt
     6781 tagcaaatga atggaatata ggaaattcac ttctatacaa tgaaattgga tttgaagaat
     6841 tctataaaac gatagatcag cttttaaaag gacaacattt aggaagaact attttaaaag
     6901 tataagtgtg agtattcatt aatacattta ctattaattc aatcattttg catttctcta
     6961 ctaaccagtg ttataattta tataacgaaa atgattaaca attaatgcac caatccccac
     7021 actattgcag tagtgtgggg attttttcga tgctggctta taaagtcgcc tattaattat
     7081 ttgttgcgtt tactaagcca ataagtataa tacgcaacag cacagccact gatgactggt
     7141 gctatgatgt gaacgaaaat gattagcaaa gtaatgcacc tcctctcttc gtcaattgac
     7201 gcctgagaga taggcgactc tattattata ccatctgtga atatacaagc atatctactt
     7261 ccgataagtc gtcttatgtt atataacttt gttttttata gtatcaagac aagaagaaac
     7321 tcgtttcaaa aaccctcatg atttttagag aaaaactctc ttatttcatc gtctttaata
     7381 aaataatatg ccatctttcc atctttataa aaatcaagta cctcattttt atataaaagc
     7441 cttaaatgat gtgaagttga agcaacactc attttcaata tcaaagaaat atcacaaaca
     7501 cacaactcat cttctttaat caaagataat atgattttca atttcttctc atcacaaatt
     7561 ttttctaaaa tgttaagtaa tttcttagat ttatcatctt ctaaaaaact taaagcattg
     7621 ttaactttat cctcatgtac acagatcaca tcacaagtat tttcataact catataaaat
     7681 cacaccctaa attaaaatat aaatcctaaa attgtttgaa tagtgtcatt ttcaataata
     7741 ataaataaac ctaaagctat ataaataata gccataatcc aacgactaaa tttctcaaca
     7801 atttctccaa ttcctggaat attagctaat ttttgtgcag taaacaccaa gaagaaaatt
     7861 aaaattaaaa agacaaacag agtaagtaat aaattagtaa cacttaatgt cacaaaatac
     7921 ggaacaaata aaccaatatt atcggcgcca caacttgcta tcgtaacaat tgcaaccgta
     7981 ccaactaatt tagacaatcc tttttcattc aattcttttt tagctctctt ttctccttca
     8041 caatcatcat aaatagccac tttaattcca agataaattg gtattaaacc caataaacct
     8101 aatatccact tttctggaac ataatttaat acaaaagcta aaaacaaact aactaatatt
     8161 aaaataatag accctaaata ttgaccaaca taaatatctc tatattcttt tctagtcttt
     8221 gctctagcaa aaaatattaa taaaattact aataaatcaa ctgctgtagc aatataaaga
     8281 acagcagcag caacaaccgt ttgaatcata atacacctaa ttcaaaaata tttttgaatg
     8341 tattataact tatattttta aataatcata ggtg
//
LOCUS       DALAAR010000101         8283 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000101 DALAAR010000000
VERSION     DALAAR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.103"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            1..978
                     /gene="guaC"
                     /locus_tag="O2458_002452"
     CDS             1..978
                     /gene="guaC"
                     /locus_tag="O2458_002452"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCZ3888295.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1000..2016
                     /locus_tag="O2458_002453"
     CDS             1000..2016
                     /locus_tag="O2458_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ3888296.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2396..3019)
                     /gene="lexA"
                     /locus_tag="O2458_002454"
     CDS             complement(2396..3019)
                     /gene="lexA"
                     /locus_tag="O2458_002454"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCZ3888297.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3162..3395
                     /locus_tag="O2458_002455"
     CDS             3162..3395
                     /locus_tag="O2458_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888298.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            3531..3770
                     /locus_tag="O2458_002456"
     CDS             3531..3770
                     /locus_tag="O2458_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ3888299.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            3891..5879
                     /gene="tkt"
                     /locus_tag="O2458_002457"
     CDS             3891..5879
                     /gene="tkt"
                     /locus_tag="O2458_002457"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCZ3888300.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            6157..6399
                     /locus_tag="O2458_002458"
     CDS             6157..6399
                     /locus_tag="O2458_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCZ3888301.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            6386..6484
                     /locus_tag="O2458_002459"
     CDS             6386..6484
                     /locus_tag="O2458_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888302.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            6578..7045
                     /locus_tag="O2458_002460"
     CDS             6578..7045
                     /locus_tag="O2458_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCZ3888303.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            7169..>8283
                     /locus_tag="O2458_002461"
     CDS             7169..>8283
                     /locus_tag="O2458_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ3888304.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRK"
BASE COUNT         3042 a         1128 c         1491 g         2622 t
ORIGIN      
        1 gtgaaaatat ttgattacga agatattcaa ttaataccta ataaatgcat agttgaaagt
       61 aggtctgaat gtgatacaac tatccaattt ggtccgaaaa aattcaagct acctgtagtt
      121 cctgcaaata tgcaaacagt tatgaatgag aaattagcga aatggtttgc tgaaaatgat
      181 tacttttata tcatgcatcg ttttgatgaa gaagcaagaa taccttttat aaaacatatg
      241 caaaattcag gcttatttgc atctatttca gttggtgtaa agaaagcgga atttgatttt
      301 attgaaaagt tagctcaaga aaaattaatc cccgaatata ttacaataga tattgcgcat
      361 ggtcactcag attcagtgat aaacatgatt aaacatataa aaacccatat acctgatagt
      421 tttgttattg ctggtaatgt tggtacgcca gaaggtgtta gagaattaga aaatgctggt
      481 gctgatgcta ccaaagtcgg tataggtcct ggtagagttt gtattacaaa gattaaaaca
      541 ggttttggta ctggtggttg gcagttagcg gcattaaaca tatgtagtaa agcagctcgt
      601 aaacctttga ttgccgatgg tggtataaga acgcatggcg acattgctaa atcaattaga
      661 tttggtgcat caatggtcat gattggttca ttatttgcgg cacacgaaga atcacctggt
      721 gaaactgtag aacttgatgg taaacagtat aaagaatatt ttggtagtgc atctgaattt
      781 caaaaaggcg aacataaaaa tgtagaaggt aaaaaaatgt ttgtagaaca taagggttca
      841 ttaatggata ccttaaaaga aatgcaacaa gatttacaaa gctcaatttc atatgccggt
      901 ggaaaagact tgaaatcatt acgtactgta gattatgtta ttgttagaaa ctctattttc
      961 aacggtgata gagattaata tttatagtag gtgatgtaaa ttaaaaaatt catagtaact
     1021 gttgttgcgt ttttatcaat tattatcatt gcgccaataa cagaatttaa accattcatt
     1081 catttacaaa atgaagtaag acaatatatt gacattcaca tcaataaaga aacaatttct
     1141 gcggaaaata aattggatac accgaagaaa caacaatttg cctttaataa tatacaaatg
     1201 aacatgtcga aatcagatgt tgagaaaaca ttaaataaac caaaaagagt gacatttaat
     1261 gaatatggta cgaagtggta tacgtattat gatgacgatt acaataattt tataatgata
     1321 agttacatga aagataaagt taatgcgtta tatacaaatc aaaatataat cacttcaaaa
     1381 tcaaaaatta aatataatac acctaaatcg gttgtaaggc aaagattagg cgaaccagaa
     1441 acagagattg ttaaaggtag agtgcgttac gaacaaaata ataaagaata tgatgttttc
     1501 cataaaaatc acatttatac gacggtattt tatgataagc atcgacgtaa taatgtaaca
     1561 gctgttttac aagtaagtga tgctatggaa aatagattaa aagaacaata tggagcacca
     1621 tcgaaatcgc ttgcagatag ttttgaacta caaaattttg atttagttaa tgctgaaaga
     1681 aaacaacatc aattatctac attgaagtat tctaaacaga attctgaaac tgcacgtaag
     1741 catagtaaag atatggccaa caatcattat tttgatcata caaatttaaa aggtcaatca
     1801 ccatttgatc gattgaaaaa agatggtatt acatttaact cagccggaga gaatttagca
     1861 tatggtcaag ttagtagtat ctatgcacat caaggattaa tgaattctat tggtcacaga
     1921 aaaaatattt taaatgatac gtttaaaata ttaggtgttg gtgttgattt taatgatgaa
     1981 aaacaacctt tttggacaga aaattatact ggttaatgtt tttggcatga gattaaaggg
     2041 taatgtttgt ctgtaaggaa gtagataaac atgatgacaa cagataaacc aaaagatgcg
     2101 gatatacttg aacgcgtcaa agatatactt aataaaaaag aaagaaaaaa ataataatgg
     2161 ctcgctcctg taaattatta cgggggcgag ttttaatttg tgtaacattt tgcagaaatc
     2221 tgtaattcaa aatttatatt aaagaatgag attatctaac aattcgcaat ttctgtttga
     2281 actgaattta gattagaata aataaaacct cttgaaaacg taactttttc aagaggtcca
     2341 aaatttataa aaatgagggc ttaacaatta cgataatata taacggttta aaatattaca
     2401 tttcgcggta caaaccaatt actttcccaa ttacagcaac attgtctagg taaattggct
     2461 ccattgtact attttcaggt tgtaatcgat aacgattttt ttctttatag aagcgtttga
     2521 cagttgcttc atcttcctca gtcatagcaa caataatgtc tccattttct gctatggttt
     2581 gactgcgaac aattactttg tctccgtcta atataccagc ctcaatcata ctgtcgccta
     2641 cgacgtttaa tatgaatatg tcgctattgt gtgtcgatgt taagtgttca ggtaatggaa
     2701 aatattcttc aatattttct actgcggtaa taggaacacc tgctgtgact ttaccaataa
     2761 ctggcacatg aatcgtttct tccatattaa tattatcatt tgtttgatca cttacaattt
     2821 ctatagcacg tggtttcgtt ggatctcttc ttatatagcc tttttcttca agacgtgaaa
     2881 ggtgaccatg aacagttgaa ctggatgcta agccaactgc ttcaccaatt tcgcgaacac
     2941 taggcggata acctttcgtt tgaacaactt gtttaatata gttatatatt tcgctttgtc
     3001 gttttgttaa ttctctcata tataggcact ccctaaatta atttaaatac agtatatcat
     3061 taatttgaaa ccaaaacaaa catttgttcg ttaaaatatt gacacagaac ataagttctg
     3121 ataaattact aatacaaaca aatgttctag gagtgaaaat gatgttttac aataaatata
     3181 aaaacgtatc aacatatatc atcatatttt tagtttcaag tgcagccttt gcaatattct
     3241 tgttaagtgc gaacattagt gctcactcgg aacaagtgta cgaaatgact gaccatcaaa
     3301 ttaagaacaa tacgataaat aaagcatacg aacataaaga ccctacaaac aatagcgaac
     3361 aaagagatgg gaaagtgttc gctttaataa attgatacat tgtcacaacg ttattttgcc
     3421 tatttttgcg aaatagcgtt ttttattact tttttgctga tcttaaattt gttatatttt
     3481 gttaaagtat tataatgatt gaataaacaa attgaaggta ggttttttaa ttgagtaatt
     3541 ctgatttgaa tatcgaaaga attaacgagt tagctaaaaa gaaaaaagaa gtaggattaa
     3601 ctcaagaaga agcaaaggag caaacagcct taagaaaagc ttatcttgag agttttagaa
     3661 aagggtttaa acaacaaatt gaaaatacta aagtaattga tccagaaggt aatgatgtaa
     3721 cacctgaaaa aattaaagag atacaacaaa aaagagataa taaaaattaa atcacaaatc
     3781 tgtaaagaat tttctgacat tataacttga aataagtatt ttacttatct ttttatttta
     3841 aaataagtta taatgtattt gataaaattg aagaagggaa gatacacaag atgtttaatg
     3901 aaaaagatca attagctgtt gatacgctac gtgcactaag tatcgacaca atcgaaaaag
     3961 cgaattctgg tcatccagga ttacctatgg gagctgcccc aatggcttac actttgtgga
     4021 cacgtcatct gaattttaat ccacaatcta aagattactt caatagagac cgtttcgtat
     4081 tatctgcagg gcatggttca gcattattgt atagcttgtt acatgtttct ggtagtttag
     4141 aattagaaga attaaagcaa tttagacaat ggggttctaa aacaccaggt catcctgaat
     4201 acagacatac agatggtgta gaagttacta ccggaccact tggacaaggt tttgctatgt
     4261 cagtaggatt agctttagca gaagatcacc tagcagggaa atttaataaa gaaggatata
     4321 atgttgtaga tcattacaca tatgtattag cttctgacgg tgatttaatg gaaggtatat
     4381 cgcatgaagc agcttcattt gctggacata ataaattaag taaattagtt gttttatacg
     4441 attcaaatga tatttcatta gatggcgaat taaacaaagc tttttctgaa aacacaaaag
     4501 ctcgttttga agcatatggt tggaattact tactagttaa agatggtaat gatttagaag
     4561 aaattgataa agcgattact acagctaaat ctcaagaagg accaacgatt attgaagtta
     4621 aaacaacaat cggatttggt tcaccgaata aagcaggaac taatggtgtt catggggcac
     4681 ctttaggtga agttgaaaga aaattaacat tcgaaaatta cggtttagat cctgaaaaac
     4741 gttttaatgt ttcagaagag gtatacgaaa ttttccaaaa tactatgtta aaacgtgcta
     4801 atgaagatga atctcaatgg aattcattat tagaaaaata tgcagaaaca tatcctgaat
     4861 tagcagaaga atttaaatta gcgattagtg gtaaattgcc taaaaattat aaggatgaat
     4921 taccacgttt tgaactgggt cataatggtg catctcgtgc tgattctggt actgttattc
     4981 aagcaatcag taaaactgtc ccttcattct ttggtggatc agcagacctt gctggttcaa
     5041 acaaatccaa tgtaaatgat gcaactgatt atagttctga aacacctgaa ggtaaaaatg
     5101 tgtggtttgg tgtacgtgaa tttgctatgg gtgctgctgt aaatggtatg gctgcacatg
     5161 gaggtttaca tccatatggt gcaacattct tcgtatttag tgattattta aaaccagcgt
     5221 tacgtttatc atcaattatg ggattaaatg caacgttcat cttcacacat gattcaattg
     5281 cagtaggtga agatggtcct actcatgaac caattgagca attagctgga ttaagagcca
     5341 ttccaaatat gaatgttatc cgtcctgctg atggtaatga aacaagagta gcatgggaag
     5401 ttgccttaga atctgaatct acacctactt cattagtatt gacacgtcaa aacttaccgg
     5461 tattagatgt accagaagat gtagttgaag aaggcgttcg aaaaggtgcc tatacagttt
     5521 atggctctga agagacacca gaattcctat tattagcttc aggttcagaa gttagtcttg
     5581 cagttgaagc tgctaaagat cttgaaaaac aaggtaaatc agtacgtgtt gtttcaatgc
     5641 ctaactggaa tgcatttgaa caacaatctg aagaatataa agaatcagtt attccatcaa
     5701 gcgtaacaaa acgtgttgcg attgaaatgg cttcaccgct tggatggcat aaatatgtag
     5761 gtactgcagg taaagttatt gctattgacg gctttggcgc aagtgcacct ggcgatttag
     5821 tagttgaaaa atatggattt acaaaagaaa atatcttaaa ccaagttatg agcttataag
     5881 aataatttat aaagcgagta tgtttagaag tctaggatgc ataatcttag gcttctttta
     5941 aagtgttgaa atttagagta tagcacttaa actacatcat aagtgataag ttatgaaagt
     6001 atactatttc agattaatct ttaaaagctc tgttataaca gcatgatttt tgatattatt
     6061 tttagtatcg atattaaaat acttgaataa actagttctt gaaataatgt gatgaattta
     6121 gtaaaattca gtaggataaa gaaagcgggt gaaacaatgg caacttggtt agcaattatt
     6181 tttatagtag ctgcattaat tttaggttta attggaggtt tccttttagc tagaaaatat
     6241 atgatggact acttgaagaa aaacccacca atcaacgaag aaatgcttcg tatgatgatg
     6301 atgcaaatgg gtcaaaaacc ttctcagaag aaaattaatc aaatgatgac gatgatgaat
     6361 aaaaatatgg atcaaaatat gaagagtgcg aaaaagtaaa ttcgcaattg atagaggcta
     6421 ttttccagat atggaaatgg cctcttttta taatcaaatt aataagaata aatatgttta
     6481 ttaaaattaa agttaacaaa atgacgaata gactgagaaa tgctataatt cattttgtat
     6541 gatttacaga gagtttattt aacgagaagg tgtctgcgtg ctctatttaa tattttcaat
     6601 cattgtagct ttatttatgg gaactatagt tatagttatt cgtatgaaag ctcaaaatta
     6661 tccggtaaat gagaaaaaaa tagttttgcc accgtttttt atggcgaccg gtgcattgat
     6721 gtacgtcgtt ccatatttta ggctaacagg atcggaaatg ctagaagcct ttataattgg
     6781 tttgcttttt tctacagttc taatttggac ttctcgattt gaagtcaaag gtacagaaat
     6841 ttatatgaaa cgatctaaag catttccagt tattttgatt tcattactta tcattcgtac
     6901 tgtgatgaaa atattcatta gtaatgaaat agatcctgga gaattaggcg gcatgttctt
     6961 tttattagca ttctgtatga ttgttccttg gagagcagca atgctatata aatacaaaaa
     7021 actaaagaaa acattaatca attaattact tttaaaacca cttgtgatcg acttctaaat
     7081 cagtcaatga gtggttttaa ttttacttga aaaggtgaaa tgattatatt aaaataaaag
     7141 cgaacaaatg ttctatatgg aggcagtaat gaaaattata catacagcag actggcactt
     7201 agggaaaata ttaaatggca aacagctttt agaagatcaa gcgtatattt tagatatgtt
     7261 cgtagaaaaa atgaaagaag aagaacctga tatcattgtg atagctggag atttatatga
     7321 cacaacatat ccaagtaaag atgcaatcat gttattagaa caagcgattg gaaagttaaa
     7381 tttagaactg cgtataccaa taattattat tagtggaaat cacgatggta aagagaggtt
     7441 aaactatggg gcgagttggt ttgaacataa tcagttattt ataagaacag attttacatc
     7501 gattaattca ccaatagaga taaatggggt taatttttat acactccctt atgctactgt
     7561 gagcgaaatg aaacactact ttgaagatga caccattgaa acacatcaac agggaattac
     7621 gcgctgtatt gaaacaatag caccggaaat tgatgaagat gccgtcaata ttttaattag
     7681 tcatctgact gttcaaggtg gaaagacatc tgattctgaa agaccattaa ctattggaac
     7741 ggttgaatca gttcagaaag gtgtttttga tatatttgat tatgtcatgc taggtcactt
     7801 gcatcatcca tttagtatag aagacgacaa aattaaatat agtggctcct tattgcagta
     7861 ttcattttcg gaagcgggtc aagctaaagg gtatagacgt gtaacaatta atgatggcat
     7921 tattaacgat gtatttattc ctcttaagcc acttagacaa ttggaaatta tctcaggcga
     7981 atataatgat gttattaatg aaaaagttca tgtgaaaaat aaagataatt atttacattt
     8041 taaacttaaa aatatgtctc atattactga tccaatgatg agtttaaaac aaatttatcc
     8101 taatacttta gcgctgacga atgaaacttt taattacaat gaagaaaata atgctataga
     8161 aataagtgaa aaagatgaca tgtcaattat cgaaatgttt tataaacata taactgataa
     8221 agaattatcg gatatccaat ctaaaaagat aaaaaatatt ttagaaaacg aattgagaaa
     8281 gga
//
LOCUS       DALAAR010000102         8113 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000102 DALAAR010000000
VERSION     DALAAR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.104"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            61..381
                     /locus_tag="O2458_002462"
     CDS             61..381
                     /locus_tag="O2458_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCZ3888305.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            378..539
                     /locus_tag="O2458_002463"
     CDS             378..539
                     /locus_tag="O2458_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCZ3888306.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            634..894
                     /locus_tag="O2458_002464"
     CDS             634..894
                     /locus_tag="O2458_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ3888307.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            934..1194
                     /locus_tag="O2458_002465"
     CDS             934..1194
                     /locus_tag="O2458_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ3888308.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1203..1466
                     /locus_tag="O2458_002466"
     CDS             1203..1466
                     /locus_tag="O2458_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888309.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1475..3418
                     /locus_tag="O2458_002467"
     CDS             1475..3418
                     /locus_tag="O2458_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ3888310.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3420..4340
                     /locus_tag="O2458_002468"
     CDS             3420..4340
                     /locus_tag="O2458_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCZ3888311.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4421..5038
                     /locus_tag="O2458_002469"
     CDS             4421..5038
                     /locus_tag="O2458_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ3888312.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5039..5509
                     /gene="ssb"
                     /locus_tag="O2458_002470"
     CDS             5039..5509
                     /gene="ssb"
                     /locus_tag="O2458_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ3888313.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5539..6432
                     /locus_tag="O2458_002471"
     CDS             5539..6432
                     /locus_tag="O2458_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ3888314.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            6439..6657
                     /locus_tag="O2458_002472"
     CDS             6439..6657
                     /locus_tag="O2458_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888315.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6666..7070
                     /locus_tag="O2458_002473"
     CDS             6666..7070
                     /locus_tag="O2458_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCZ3888316.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7083..7451
                     /locus_tag="O2458_002474"
     CDS             7083..7451
                     /locus_tag="O2458_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888317.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7455..7697
                     /locus_tag="O2458_002475"
     CDS             7455..7697
                     /locus_tag="O2458_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCZ3888318.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7706..8077
                     /locus_tag="O2458_002476"
     CDS             7706..8077
                     /locus_tag="O2458_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ3888319.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8061..>8113
                     /locus_tag="O2458_002477"
     CDS             8061..>8113
                     /locus_tag="O2458_002477"
                     /inference="COORDINATES: protein motif:HMM:NF018019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ3888320.1"
                     /translation="MTQMNNREQIEQSVISA"
BASE COUNT         3344 a         1226 c         1501 g         2042 t
ORIGIN      
        1 atacttatca cctcctttca ctaggagata acaacattat acacgaaagg aaagatagaa
       61 atgccacaca ttttaaacgt aacagttcca atacctgaaa cacatgtact tatcacaaaa
      121 gatgaatatg atgagctaat tggttattca ttagaccctg tatggaacat gagtgactta
      181 aagaagaaat taaaaattgc atctgatgag actatcaagg acagattact atttcatcct
      241 agatttgaaa aagaactaag agcgcaagga attgtgcatt acccagatga gagttttaat
      301 cgctggagat ttaacgcaag aaagatgaat aaattcgtcg atgagcattt caatgaaata
      361 tataaggaga gaataaaatg agcgacacat ataaaagcta cctattagca gtgctatgtt
      421 tcacagtctt agcgattgtg cttatgccat tgctgtactt cactacagcg tggtcaattg
      481 caggattcgc aagcattgga acattcatat actacaaaga atacttttat gaagaataaa
      541 aaaactgcta cttgcgtcaa caagtaacag tgacaaacga ttaacaaaat taattcattt
      601 tcaatataaa acgaaacaag gaggaagtca accatgacta aaaattataa agatatgact
      661 caagacgaat taaggggttt attggctgaa aagaatgcag aattgtttga tttagcgagc
      721 gaaatcgatg aagaaactga atttgacgtt ttactttttt caaatgtagg gattagcaac
      781 ggagatttta caccgtcgtc acattgtgtg attgggaatg ttgttgatat tgctaattta
      841 ttgaaacgta gagctgttta ccgagatatc gctgatgtta tcaaaatgcg ttaaaaattc
      901 tagctataga taatgacaag gaggacgaga acaatgtatt acaaaacggg tgacgtatgt
      961 cgaaaaataa ttaatgtaga tggctttgat tttcaattaa gagttaagaa acgagcgtat
     1021 agcgtcgaaa tggttgttct agaccatgaa ggaaattcaa ttgacgggtt actagtttct
     1081 gatgagaacg atctatacac agctttagat attttgaaac aaagtgttta tgaatggatt
     1141 gaaaataaca cagatgaaca ggacaaacta atgaacttag tcatgaaatg gtaggtataa
     1201 gcatgagaga tacagaaaga aatatattga atatttttaa gacgttattc gacgaatata
     1261 ctttgtcaaa ccaacgagca ttattggaaa ttgaacgtaa tcatcacgga tacttatcga
     1321 ttaatttctt gcactatcac gacagttaca aaacaaacaa taagcttgtg cagatacatg
     1381 aaatcaatcc agacagccat gaacgaataa aaaatttaat tatcgaggta ctaagaggtc
     1441 atcggaagat taaaaaagga gcatgaggaa ggatatgaaa ataaataaat taacaatatc
     1501 gaactttgcc ggaatcaaag aagaaaaatt taattttgac ggtaaagatg caaaaatata
     1561 cggcaataat gcgactggta agactacaac agcaaccgca ttacaatggt tgcttttcga
     1621 taaaggtttg gacggttcaa ccaaatcatt taaccctgta cctttaaacg aaaaaaacga
     1681 agaaaattat gagttaattc cgactgtttt cgcagaattt gaaatcgacg gaaaaattac
     1741 gacttttaaa aaagagtcac atcctaaata cacaataaat caaaagacga atcgcaagga
     1801 atactcacga agtcgaacga agaaacaata tatcaatgat gaatcaataa aagtaaagga
     1861 ttataaagct cgtattgatg aacttattga tgaagatgta ttcaagttaa ttacgaaccc
     1921 tcaagcattt aacttactag attggaagaa gcgaagaagt ttgttgtttg aaattgctaa
     1981 accaatcaat gatgaggatg tcattaaaac aaatgatgat tttaaagaac taaataatat
     2041 tcttggagat catgaaattg aaacaaagaa aaagattctt acggacaaga taaaacagat
     2101 taacaaagat atcaaagata ttccgatacg tattaaccaa acacaacaaa ataagcagga
     2161 tgtaccagaa ttcgataatg atagatacgc aattatcaaa caagaaattg agcaacttga
     2221 aaatgagcgt atagatattc aaaatggtaa ggaagaaatt aatttacgta atcaattagc
     2281 tgataaacaa tcagaattga aacgcataga agacaataac agcgcaagta atgagaacaa
     2341 aatccatgct ttaacaaatg aattacacgt tgaaaatgga acggtagcaa accttaaaac
     2401 gagattaaag caaaacaaac aacaaatcac gcatgaagaa aatagacgta atcaattatt
     2461 agaaaatcat aaaggattaa aaagtgattt agaaaaagct aaaaatcaaa aatttgaata
     2521 tcttgatgac aatgtatgta gttgttgtgg tcaaaagtta ccagttgaac aagtggatat
     2581 ggcaagagaa aaagcattgc agaaattcaa tgctagcaaa tcgaaagaat tagaaacaat
     2641 acaaacatct atcaatcaca ttatttcaga aggcaagaaa ataaagctaa tcatcgagaa
     2701 gttagaggat gacaataata atcttcaaat taaaatcaac gaagcagaag agcgttcagc
     2761 aagaatacaa aacaaaatta ataagttgaa aacaactcac gttgacgtta cgcaaactga
     2821 cgaatacaaa gcagtaatgt tagagataaa tgagattaat caaaaacgct ctaacatcag
     2881 gaaaactatt caagataaag tttcaggaat agatgacaaa ataagcgaac ttactcaaga
     2941 aaaatcagaa attgaagtgt caagatcaat cgaaaaatca aataaacatc tagatgatgt
     3001 tatttctgaa ttaagaaatg aagaagacag attattggat gaaaaagaaa agtattcaca
     3061 tgacctttat atcttaaaag aatttacaac aacaaaagtc aaaatgctta ctgaaaacat
     3121 caataatgaa tttgaaattg ctgaatttaa gctattcaat accttagtta acggcgaatt
     3181 agaagaaaca tgttccacaa cggttaacgg cgtcgaatac gacagcggtt taaataacgc
     3241 ctcaagaatt aatgttggct tagatatcat caatacactg tcaaaacatt ttaaagttac
     3301 agcgccaata tttattgata atgctgaatc agtaacagag cttatcaaaa cagaatcaca
     3361 acaaattcaa ttgatagtaa atgaacaaga taaaaaatta agaatggaga ctatataaaa
     3421 tgactgaaaa taataaatta caaactattg aacaacaatt agtacaagaa aagaacgtat
     3481 ctgacaacgt attaaacaaa gtgagagttt tagagtcaca aggcaatttg gaattgccaa
     3541 atgattattc accaagtaat gccatgaaac aagcatggtt acaaatcagc caagataaca
     3601 aattaatgag ttgtaacgat acaagcaaag caaatgcctt attagacatg gtaacgcaag
     3661 gtttaaatcc agctaaaaat caatgctact ttattcctta cggcaacaaa atgcagttac
     3721 aacgtagcta tcacggtaat gtaatgatgt taaaacgtga tgcaggtgct caagatgttg
     3781 ttgctcaagt gatttataaa ggcgatacat tcaagcaaga aatgggagaa acaggacgta
     3841 tcaaagcgat taaacacgaa caagacttct ttaacatcga caaagaaaac attatcggtg
     3901 cgtactgcac aatcgtattt aatgatggac gagataacta tattgaagtc atgactattg
     3961 aacaaattaa acaagcatgg atgcagtcat caatgattaa agatgaaaaa gcattacaaa
     4021 attctaaaac acataataat ttcaaagaag aaatggctaa aaaaacagtt atcaatagag
     4081 ctgctaaacg ttatatcaac acatcaacag atagcaatct tttcaaatac gcacaagaat
     4141 ccgaacaacg tcaacgcaaa gaagtgttgg acgcagaagt tgaagaaaat gcaaatcaag
     4201 aacaattgga ctttgaacaa ccagttcttg aagaagcaca atacacagaa ttagaaaatg
     4261 ataagcctat tgatgtatct gactttgaag aaataaaaga acctgcaaca gaaaaagaaa
     4321 gcgaagaaga gccattttaa ttgaaacaat agcaactggt tcaagtggta actgctacgt
     4381 cttaaatgat ggacgtacta cgttactact tgaggcaggt ataaaatttg aacgtgttca
     4441 aaagcatttc aaatataaaa caagacatat agcagggtgt cttatcacac acgaacatgg
     4501 tgatcatgca aagtatacaa agcagtttgt cgacaatggt gtaatcagct atatgactgc
     4561 tggaacacaa caagctatga attttgaaag tcatcgctta tgcacgatta aggcaaagca
     4621 agagctgcga ataggtacat ggtcaattct accgtttgac atcgaacatg atgctaacga
     4681 gcctgtggct ttcttattac aaagcacact aggttataag gttctgtatg ttactgatac
     4741 aaagtatttg aaatacaaat ttaatggcat tacgcacatg atgctagaag ttaattatat
     4801 ctatgaacaa atgcaagaaa acataaaaaa cggcagtgtg cacagcacat tagcaaacag
     4861 aattatggag tctcatttta gcttagaaca tgctatcgga atgttaaaag caaatgattt
     4921 aactagactc gaagaaatac atttaattca tttaagtagt caaaattcaa atgcaaaata
     4981 cattaaaagt gaaatacaaa aagtgacggg cgcgcccgtt tattttggag gtttataaat
     5041 gctaaacaga acaatattag ttggtcgttt aactagagac ccagaattaa gaaccactca
     5101 aaatggtgta aatgtagcat cattcacatt agcagttaac cgtacattta caaatgcaca
     5161 aggcgagcgc gaggcagact ttattaatat catcgtattt aaaaaacaag cagagaacgt
     5221 taataaatac ctatctaaag gatcgttggc gggcgtagat ggtaggttac aaacgcggaa
     5281 ctatgaaaat aaggaaggtc aacgtgtata cgttacggaa gttattgctg atagtattca
     5341 atttttagaa ccgaaaaact caaatgacac tcaacaagat ttatatcaac aacaagtaca
     5401 acaaacacgt ggacaatcgc aatattcaaa taacaaacca gtaaaagata atccgtttgc
     5461 gaatgcaaat ggtccgattg aactaaatga tgatgattta ccattctaat ttaaccggtt
     5521 tgaaagtgag gtgtgtatat gactggttgg ataagtattg atcgctcaat tcaaaatcat
     5581 tggctattta aagaaaagag aacattttca aagtttgaag catggatata tttactcatg
     5641 gaagcgaatc attcaaaggc aaaagtgcct ataggaaacc aaattgtaac cgttgaaaga
     5701 ggacaaagat taacatctat tttgaccttg tctgaccttt ttaactggtc acgatttaaa
     5761 gtgaaaacct tccttgactt actcgagagt gatggaatgt tagaagtcaa aacaacatca
     5821 aaatataccc ttataaccat tgtcaattat gacttttatc aaagtgagca gggcaggaac
     5881 caacatcaaa acgacatcaa accaacatca aaacaacatc agtcaaacat caacccaaca
     5941 tcaaaacaac atcaaaccaa cacaaacaat aatgataata aagataataa tgaaaagaat
     6001 gtgaataatg agaagaagaa gacaaccgcc ttcgacttct tccaagataa cggattcggt
     6061 ttcataactt cttacaattt agacgattta aattattatc ttgattcatt tgaaaatgat
     6121 tcagatgaaa tagttaccgc atcacttaaa atcgctaaag acagaaacaa agttacttgg
     6181 ggatatgcta aaagcatttt gaatacatgg cttaatgcaa acttgaaatc tattgaacaa
     6241 gtacgtgcat ttgaaaagca acaacttgaa agcaaaaaac aaaattataa acctttcgtt
     6301 aaacaatcaa aagaaaaaac acccaaatgg ctcacagaca gcacgagaga aacgaaaacg
     6361 ccggaagtag atgaaaacct tgagaaagac agagaagctt ttattaagcg tctaaatagc
     6421 aaatgggagt gattgaaaat ggatgcattt gataaatact atctatttga tcatgacggc
     6481 aacaaaatgt tttcagttac accacatttt aaagatggac ggcatttagt tgttggatta
     6541 aaacacacaa aattcaatgg tcgacgttgg tacttagatg attatgaatt aaaaacactt
     6601 attgataatg aacaaatgga gttaggacac caaacaagct tatttgaata tatatgaggg
     6661 attacatgga gatagaaatt aaatttaatg aagtgtttaa tgcgccgatg ggctcgcctc
     6721 gaccgcgttt tagcacaaaa ggtagatatg cacacacata tatgcctaca aaatatacag
     6781 aacataaaaa atatttacaa aatcaaatgc caaagctaaa tctagaaaat gcattaaaaa
     6841 ttgaattaga gttttacttt actccaccta aatcatggtc gaagaaaaag aaaactcaag
     6901 cgattggaca attaaaagta actaaaccag atatcgataa tttgatgaag acagttctag
     6961 acgcttgtaa caattatttg tggaaagacg ataaccaaat tacagaaata actagctcaa
     7021 agcgttatgg aattgagccc aaaataatca tacgaataga agaaatataa gaggtggata
     7081 aaatggcgag aaaagcaagg attgtaacaa taaatgataa accatatagg tttaccaaat
     7141 ctgaaatgga attaatagaa agtcacggta taaccgctgg aatggtttct aagagagtaa
     7201 aagacggttg ggaactacat gaagcaatgg acgcaccaga aggtacgcgt ttaagcgagt
     7261 acagagaaaa gaaaacaata gaaagactgg aacaagctag actcgaacgc aaattggaaa
     7321 gaaagcgaaa gaaagaggct gagctaagaa gaaagaagcc acatttgttt aatgtaccac
     7381 agaaacatcc aagaggacgt tatgcgtgct acctgatgga aaacgacata ttcgtgaaag
     7441 ttaagaagta gatcatgaca gataacgcac gcaaagaata cctaaatcaa ttctttggat
     7501 ctaagagata tctgtatcag gataacgaac gagtggcaca tattcatgta gtaaacggca
     7561 cttattactt tcatgggcat atcgtaccag gttggcaagg cgtgaaaaag acatttgata
     7621 cagctgaaga gcttgaaaca tatataaagc aacagggttt ggaatatgag gaacagaagc
     7681 aactaacttt attttagagg aggttatgaa agtgaactat gaaacaggat tccaaatagg
     7741 cgtaatggaa gctaggttga agaagatgag aaaacaacgt gatgagtaca agaagcaacg
     7801 tgacgagctt attggggata tagctaagtt aagagagcgt aacgaagagc tggagaacat
     7861 gtggcgcacg cttaaaaatg aattgtttgg aagatacgaa ttttaccgtt ttagacttag
     7921 cgaactacag attgagagca gagcgaacaa ggaagtagct atatatagaa gagctgaaat
     7981 caacttaagt gttatattgt gccgaatgga caaactagac ggaacaaatg agttctacga
     8041 atttttaggg caaatggagg atgacacaaa tgaataaccg cgaacaaatc gaacaatcag
     8101 ttatcagtgc tag
//
LOCUS       DALAAR010000103         7783 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000103 DALAAR010000000
VERSION     DALAAR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.106"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            47..682
                     /locus_tag="O2458_002478"
     CDS             47..682
                     /locus_tag="O2458_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCZ3888321.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            1061..1948
                     /locus_tag="O2458_002479"
     CDS             1061..1948
                     /locus_tag="O2458_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3888322.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            2096..3046
                     /locus_tag="O2458_002480"
     CDS             2096..3046
                     /locus_tag="O2458_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ3888323.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            3101..3820
                     /locus_tag="O2458_002481"
     CDS             3101..3820
                     /locus_tag="O2458_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3888324.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            3804..4604
                     /locus_tag="O2458_002482"
     CDS             3804..4604
                     /locus_tag="O2458_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3888325.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            4740..5246
                     /locus_tag="O2458_002483"
     CDS             4740..5246
                     /locus_tag="O2458_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888326.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            5408..6124
                     /locus_tag="O2458_002484"
     CDS             5408..6124
                     /locus_tag="O2458_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888327.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            6293..7081
                     /locus_tag="O2458_002485"
     CDS             6293..7081
                     /locus_tag="O2458_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3888328.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(7332..7706)
                     /locus_tag="O2458_002486"
     CDS             complement(7332..7706)
                     /locus_tag="O2458_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ3888329.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT         2850 a         1002 c         1350 g         2581 t
ORIGIN      
        1 cgataatatc atttctgtcc cactccctct aaaatggagg gtgtaaatgt taggaactga
       61 tgaattatat aaagttttat atgaacatct cggaccacaa ttttggtggc ctgctgataa
      121 tgacattgaa atgatgttag gtgcaatttt agttcaaaat actagatggc gaaatgcaga
      181 aattgcattg aatcagatta aagaacatac gcattttaat ccaaatcata tattagaact
      241 acctattgaa acgttacaat cattgataca ttcaagtggc ttttataaaa gtaaatcact
      301 gacgattaaa acattattaa catggttagc acgacatcat ttcaattatc aagagattaa
      361 tgagcgatat aaaggtggat taagaaaaga attattatct ttgaaaggta ttggaagtga
      421 aacagcagat gtcttacttg tttatatatt cggacgtatt gaatttattc cagatagcta
      481 tacaagaaaa atatatgata aattaggata tgaaaacact aaaaattatg atcaattaaa
      541 aaaagtagtc acattaccaa atcattttac aaatcaagat gctaatgaat ttcatgctct
      601 gttagatgta tttggtaaac attactttag agacaaagat ataaagaatt atgatttttt
      661 agaaccttac tttaaaaagt aaacgctgtg aagttagata gatgagttta tatgaaatat
      721 aaaaaataat ttactatttt cttttagtat gtggacttat ataataaata gaagcatata
      781 aagaaaaaaa cagttgtttg tttgtgcagc aactgcataa gagcccctaa tcgctaaagc
      841 tcaaggggag taaaggaata cagttgtttg tgcagcaact gcataaaagc ctctaatcac
      901 taaaggtgaa gaggaacgca gttggatgct aaggcacaac tgcataaaag cctctaatcg
      961 ctaaagatga agaggaacgc agttggatgc taccgcacaa ctgcataaat ccctctaatc
     1021 gctaaagcga aaagtgggat taaaaaggag atgtgatagt gtgaagaaat cgttaattgc
     1081 ttttattttg atttttatgc ttgtcctgag tggctgtggt atgaaagata atgataaaca
     1141 aggtagcaat gataatggct cgtctaaatc gccgtaccat agaattgttt cgttaatgcc
     1201 tagtaatact gaaattttat atgaattagg attaggtaaa tacatagttg gtgtttcaac
     1261 ggttgatgat tatccaaaag atgtgaaaaa gggtaagaaa caatttgatg ctttgaatct
     1321 aaataaagag gaacttttaa aggcaaagcc agatctaatt cttgcgcatg agtcgcaaaa
     1381 ggcaactgct aataaagtat tgtcatcatt agagaaacaa ggcatcaaag tagtgtatgt
     1441 taaagatgca caatcaattg atgaaactta caacacattt aagcaaattg ggaaattaac
     1501 gcatcatgat aagcaggctg aacaacttgt tgaggaaact aaagataata tcgataaagt
     1561 catagattca attcctgctc atcataaaaa atcaaaagta tttattgagg tttcatcaaa
     1621 gcctgaaata tatacagcag ggaagcatac attttttaat gatatgttag aaaaattaga
     1681 agcccaaaat gtgtatagtg acattaatgg ttggaaccct gtaacgaagg aaagtattat
     1741 taaaaagaac ccagatatat taatttcgac ggaagctaag acaagatcag attatatgga
     1801 tatcatcaaa aaaagaggtg gattcaataa aattaatgct gtcaagaata cacgtattga
     1861 agttgtaaat ggtgatgaag tatcaagacc aggtccacgt attgatgaag gattaaaaga
     1921 attaagagat gcaatttata gaaaataaac cattctaatt atgcccctta ttgctacatg
     1981 taaaaaatac atgtttgaga taaggggttt ttaaaatata tttagtgaat gatagcaacg
     2041 cgagtatgtg attgctataa tgaatgtaat tatcgatgaa agaaaagaga atgctatgac
     2101 atttaataaa gtattattga gctggatagt catattgatt ataacaacta gcatatatct
     2161 attttggcag ttgggcgata tcaatgatgt atttaaccag tctattttaa tcaatgttag
     2221 attaccgaga ttattagaag cattgttgac aggtatgata ttaactgttg caggccttat
     2281 atttcaaaca gttttaaata atgcattggc agatagcttt acattaggat tggcaagcgg
     2341 cgctacattt ggttcaggat tagcattatt tttaggttta acaacgttat ggattcctgt
     2401 attttcaata acatttagtt tgataacatt aataactgta ttagtcatta cgtcggtatt
     2461 gagccaaggc tatccagtta gaatcttaat attaagtggt ttaatgattg gtgcgttatt
     2521 caattcactt ctatattttt tgattttatt aaaacctcgc aaattaaata caattgccaa
     2581 ttatctgttt ggtggttttg gtgatgcaga atactcaaat gtatctataa tagcaatcac
     2641 atttatcatt gcattgtttg gtatatttat cattcttaat caactaaagt tattgcaatt
     2701 aggagaacta aaaagtcagt cactaggctt aaatgttcaa ttgattacat atatcgcgtt
     2761 atgtatagct tctatgataa cggcgataaa tgtcgcatat gttggcatca ttggattcat
     2821 tggtatggtg ataccgcaac tcattagaaa atggcagtgg aaacaatcat taggaagaca
     2881 attggcttta aatattgtaa ctggaggaca aataatggtt atggcagatt ttattggtag
     2941 ccatatattg tcaccagtac aaataccggc aagtattatc attgcattaa ttggtatacc
     3001 agtgttattt tacatgctaa tatctcagtc gaaacggtta cactagcaca cgacatttgc
     3061 taaaataaaa ataactataa acataaagag ggcataagcg atggatttga atcaaattaa
     3121 agcagttgta tttgatttag aaggtacgtt gttggacaga gttaaatctc gagagaaatt
     3181 tatcgaagag caatatgaac gatttcatga ctacttaatt catgttcaac tggcagattt
     3241 taaaaaagca tttattgagc tagatgacga tgaagataat gataaacctg atttatataa
     3301 agaaatcatt aaacgtttcc atgtagatag gttaacttgg aaagacttat ttaatgattt
     3361 tgaaatgcat ttttatcgtt atgtatttcc ttattacgat actttgtata cactagaaaa
     3421 gctatcgcaa aaaggctttc aaattggtgt tatcgcaaat ggtaaatcta agattaaaca
     3481 atttcgatta cattcacttg gtttgatgca tgttattaat tatttatcaa catcagaaac
     3541 agttggtttt cgtaaaccac atcctaaaat ttttgaagat atgattgatc aactaggggt
     3601 attacctgag caaattatgt atgttggcga tgatgcgtta aatgatgtag ctccagcacg
     3661 agctatgggc atggttagtg tatggtataa acaagaagat gctgaaattg aaccactcga
     3721 agaagaagtt gattttacaa ttacaacagt ggaagaatta ttaaccattt taccaataaa
     3781 aaatgataat aaaggagaaa attatggatc tatttactag aaaagatgga acatcgatac
     3841 attacagtac attaggtgaa ggctatccta tcgtattgat tcatactgta cttgataatt
     3901 attctgtgtt taataaatta gcagcagaat tagcaaaatc atttcaagtt gtgttaattg
     3961 atttacgtgg acatggctat tctgataaac ctcgtcacat tgaaataaaa gatttttctg
     4021 atgacattgt tgaattactt aaatatttat atattgaaga agttgcattt gtatgccatg
     4081 aaatgggtgg aatcattggt gcggatattt cagtacgtta tcctgaattt acatcatcac
     4141 ttacgttggt aaatccaaca tctattgaag gtgaattacc ggaagaacgt ttatttagaa
     4201 aatatgccca tattattcga aactgggatc ctgaaaaaca agataaattt ttaaataagc
     4261 gtaagtatta tcgtccgaga aaaatgaatc gattcctcaa acatgtcgta gatacaaatg
     4321 aaatatcaac taaagaagaa attcaagcag ttaaagaggt attcaaaaac gctgatattt
     4381 ctcaaactta tagaaatgtc gtagtaccga caaaaattat tgcaggagaa ttcggtgaaa
     4441 gaacaacaag attagaagct aaagaagtag ctgatttaat ccaaaatgcg gactttgaag
     4501 tatatcaaga atcaagtgca ttcccatttg ttgaagagca agaaagattc gtcgaagata
     4561 cagctgcatt tatcaacaaa catcacgatg aaaagcatgt ttaattttta gtgataagtg
     4621 aaatataaaa gtagtaatta ttatttgtaa tataattgta atatgactgt tgttttagaa
     4681 atgattgttg tataatggtt gtgagcacaa gacgatataa ataaaaggag agagtgctta
     4741 tgaaaaagct actaacggca agtataattg catgttctgt tgtaatggga gtaggcttag
     4801 tgaacactag tgccgaagca gcaagtggca actctattga tactgttaaa caattaatta
     4861 agggtgatca gtcattagaa aatgtgaaaa ttggcgaatc tattaaagat gttttaacta
     4921 agtacaaaaa tccgatgtat tcttacaatg aagatggaac tgaacattat tacgaattcc
     4981 atactaaaaa aggtatgtta ttagtaacta ctgatggtaa gaaaaacaat ggtaaagtaa
     5041 ctcatatttc aatgatgtat aatgatgcta atggtccaac atatcaagct gttaaaaatt
     5101 atgttggcaa agcagtaaca catacggaat atagcaaagt tgctggtaat tttggatata
     5161 ttgaaaaagg caaaacgact tatcaatttg cctcagcacc aaaagataaa aacataaaat
     5221 tatatcgtat tgatttggaa aaataataat taaattaatc gtgcttaacc gacaagttaa
     5281 atcgcaatac ttgtcggtta ttttattaca tggcataata cagtgaatat taatttttat
     5341 gatttgcgaa ggggaaatta aaccaataca ataaataaag atgtttcatt ttaaaggagt
     5401 actaccaatg cgtattaacg ataaaatttt actagaaaat atagaagatt actttaatca
     5461 taaaggttta tcaccgcatt tgattgatga tattaaagag aaagtaatta ctgatataaa
     5521 aaattctgaa aagaaagatc aagattatat tgaatataaa agaaaatctc cagcacaaat
     5581 catattaatg attcaaagga atttgtttgc tttacaaatg aatccagtta ttttctttat
     5641 tataaacttc attctcatat cctatttata tgataaacag tatgttcagt ttcaagctat
     5701 tactggaatg agtctatttt attgtttagt gatttttcca atgactattg ttgtatacct
     5761 aagggtgtct caaaagaatt acttgcgtag taataaaata gaaatgatta tgggtacaat
     5821 tatcgctatc atatccttgt tattaattat attacaagca tttaatatta cttggggcgt
     5881 tataccaatt acaaattttg gacatcaatt tttctttttc attggtatta ttttagtaat
     5941 tgccggcata ttttataagc gacttgagtt ttcgggaatc gggttattat tttgtcaaaa
     6001 aaccgtcgat gcaatgattc ataatccaca atcagcccag attttttcat taattatatg
     6061 gatattatta gtagttctag ttatatattt cacaattaga ttatcttcac gtacaagatt
     6121 ataaatatga taaaactatt cacttgatta attgtattaa ttgagatgaa tagttttttt
     6181 attgttggaa taacttttgg taatttataa ataatttaaa aaaattgttt ataaaatgga
     6241 agcgtatata gaatgaaggt tgggtatata gtttattgag ggaggtgtca caatgaataa
     6301 agtcacaatt aatcctcaaa tccaattaac ttatcaaatt gaaggtaaag gggatcctat
     6361 aatattactt catggattgg atggtaattt agctggattt gaagatttgc aacatcaact
     6421 agcatcatca tataaagtac ttacttacga tttaagaggt catggcaagt cttctaaaag
     6481 tgaatcatac gatttaaacg atcacgttga ggatttaaaa attctaatgg agaagttaaa
     6541 tattcatgag gcacatattc taggacatga tttaggtggg gtagttgcta agttatttac
     6601 agataaatat gcttatcgtg taaaatcatt aactaccatt gcatcgaaga aagatgactt
     6661 aatacacagc tttactcaat tgttaataca atatcaagat gatatagcgg gttttaataa
     6721 gtctgaagcg tatattcttt tattttctaa attgtttaga aatcaagaga agacgatgaa
     6781 atggtatcaa aaacaaagaa tatatagcat taagtctgag gatgatagtg cggtggcaat
     6841 tcgttcatta attttgcata aagatgaacc tatgtattta aaaaaacgta catgtgtacc
     6901 tactttgtta attaatgggg aacatgatcc tttgattaaa gataagaatc attttaaatt
     6961 ggaagcgcat tttttaaatg ttacgaaaaa aatcttcgaa cattcaggac atgcaccgca
     7021 tattgaagaa ccagaagcat ttatgaatta ttatttaaaa tttttaaaaa gcgtatcata
     7081 atatgtgata tataaaccta gggcataaag tccttaggca atgtgaaaaa gctgattact
     7141 attcattatt tgatagaaat cagctttttt tgaaatgtat ttgatatata ctgctcgtta
     7201 tgcggctatc ttccttatat taagtgccat tagtgcaaaa cctcttaaca attaggtaaa
     7261 aagagcataa aaaaaggaag tttaatagaa tgtatcatct atcaaacttc accaaattgc
     7321 gctaaacaaa attatagttc aatttcgttg tttgcttcag tgattcgttt atttactcga
     7381 ctcaataatg attcgatttt tttacgttgt tgtgcattaa caagaattaa tacagttctt
     7441 tcatcatgct cattacgttt tttatcgaag taatcttctt gagataaaat tttaactgct
     7501 ttaacaactt gtggttgttt gtagtttaaa tgattaataa tatctttaag atagtattct
     7561 ttctctttgt tttcgctgat gtatgtcaat acagcgaatt cttcaaagct aattgaaaat
     7621 tcctttttaa ttaaactttt taatttgtca gcataagtga ccattgataa caactcaaag
     7681 caatcattga tttttgtaat tgccatgttt aaaacctccc tatttgatgc atcttgctcg
     7741 atacatttgc ccgataatat attgatatct aatctttatt tat
//
LOCUS       DALAAR010000104         7602 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000104 DALAAR010000000
VERSION     DALAAR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.107"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            321..1622
                     /locus_tag="O2458_002487"
     CDS             321..1622
                     /locus_tag="O2458_002487"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCZ3888330.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1636..1986
                     /locus_tag="O2458_002488"
     CDS             1636..1986
                     /locus_tag="O2458_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888331.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2138..2608)
                     /locus_tag="O2458_002489"
     CDS             complement(2138..2608)
                     /locus_tag="O2458_002489"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCZ3888332.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2919..4103
                     /locus_tag="O2458_002490"
     CDS             2919..4103
                     /locus_tag="O2458_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCZ3888333.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4103..5296
                     /locus_tag="O2458_002491"
     CDS             4103..5296
                     /locus_tag="O2458_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCZ3888334.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            5695..6366
                     /locus_tag="O2458_002492"
     CDS             5695..6366
                     /locus_tag="O2458_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCZ3888335.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6496..7299)
                     /gene="coaW"
                     /locus_tag="O2458_002493"
     CDS             complement(6496..7299)
                     /gene="coaW"
                     /locus_tag="O2458_002493"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCZ3888336.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
BASE COUNT         2634 a         1064 c         1488 g         2416 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaatatgtaa
       61 gcatgggaca tcgtaaccat atataagtat gtcgatatca gctttaagac atcataaatg
      121 ccatgatgat tctaagataa tttaataggg actttaacgt tataaccaat ctatttaaga
      181 ataataacat cgcattgaaa ttgtgatatt aaaaatgaaa tcgatagctt gctataataa
      241 ttttcgcttg tgcttttaaa aatggactag agaaagcgca gatgctttgt cctaagcttt
      301 ttaagaaaag gatgataaaa atgagaatga tagacattat tgagaaaaag cgtgacggtc
      361 atacactaac aacggaagaa attaatttct ttattggcgg ctatgttaaa ggggatattc
      421 ctgattacca agcatcaagt ttagcaatgg cgatttattt ccaagatatg aatgatgatg
      481 agcgtgcagc attaacgatg gctatggtta attctggtga tatgatagat ttgagtgata
      541 ttaaaggtgt caaagtagat aagcactcaa caggtggtgt aggagataca actacattgg
      601 ttttagcacc attagtagca gctgtagatg ttcctgttgc aaaaatgagt gggcgtggat
      661 taggtcatac aggtggtacg attgataaat tagaagcaat tgatggtttt catgttgaaa
      721 tagatgaagc aacatttgtg aaattggtta atgaaaataa agtggcagtt gtaggacaat
      781 caggaaattt aactcctgca gacaaaaaat tatatgcctt aagggatgtt actggtactg
      841 tcaattcaat accattaatt gcctcttcaa ttatgagtaa aaagattgct gctggtgcag
      901 atgcaattgt attagatgta aaaactggta gcggtgcatt tatgaaaaca ttagaagatg
      961 ctgaagcatt agcgcacgca atggtacgta ttggtaataa tgtgggacgt aatacgatgg
     1021 cgattatttc tgatatgaat cagccacttg gacgtgcgat tggcaatgcg cttgagttac
     1081 aagaagcaat tgatacgttg aaaggacaag gtccgaaaga tttaacagaa cttgtattaa
     1141 cattaggttc tcaaatggtt gtacttgcaa acaaagctga aacgcttgaa gaagcgagag
     1201 cgttgctaat tgaggcaatt aattcaggtg cagcattaga aaaattcaaa acatttatta
     1261 aaaaccaagg tggcgacgaa actgttattg accatccaga gcgtttgcca caagctcaat
     1321 atcaaattga atataaagct aaaaaatcag gttatgtgac tgaattagtc tctaacgata
     1381 taggtgtcgc ttcgatgatg ttaggagcgg gacgtttaac aaaagaggat gatattgatt
     1441 tagcggttgg tattgtttta aataaaaaaa ttggtgataa agtagaagaa ggagaatcat
     1501 tattaacgat tcacagtaat cgccaagatg tagacgatgt tgttaaaaaa ttagattcaa
     1561 gcataacaat tgctgatcat gttgtatcgc ctacattaat tcataaaatc attacagaat
     1621 aggagttgaa gctgattgaa catactaaga tatttattag gaataggttt tagtgtaata
     1681 ggtgtattgc attttacacg agaacgacaa tttagaaata tcataccgaa atgtttgcca
     1741 cttcgaaaaa cagcggttct tgttacagga atctttgaga tattatttgg acttgcactt
     1801 ttaattaaaa gaccatctca atgcttgaaa aatacaatca atctattctt gctagctgta
     1861 ttcccagcta atatctatat ggcagttaaa aagttacctt taggagataa acaattgcca
     1921 acatggcaac tatatttaag gttacctatg cagttcgtgt taatgggact aattaagaaa
     1981 ttatagtggt tctcaaataa atcggacaaa tgttaaagat tttacttttt catttgttcg
     2041 attttttgac tttgaagcat aataaaaagc agttggacaa tggcgattgt aaaatcattg
     2101 ttcgactgct tttctgatta acttgattga ctaagattta ttttcctgta ccgaaaacat
     2161 catgccattc gtttcgtttg tctagaaatg cttgagcgat agttttagca ccttctaatg
     2221 aatgacttgc tgcccagcca cattgtactt cattacaagc aggcacttca gtagcattta
     2281 gcacatcatt taaagttgct tcaacaatat ttaatacatc atcataatta tcatgattaa
     2341 taaatgatac atagaaacca gtttggcaac ccataggact taaatcaaca actttgtcac
     2401 tatgatttct aatattttca gccattaaat gttctaatga atgtagtccg ggcatatcca
     2461 tatgttcttt gtttggttgt ttgaaacgaa tgtcgtattt gtgaatgaca tctccgttta
     2521 atccttccat cgttcccgct aatctaataa atggggcaac caccttagta tgatctaaat
     2581 tgaaactttc aacattcatt tttgtcattt tgaatttcct cctattaact actcaaaact
     2641 ataaaacgct tatgatttgt tgcattataa aacttaagtt tgttgtaagt tgtgtttcta
     2701 acaatactta aattgtaaca atagggctta tgttttacaa tttgaaactg ttttataaat
     2761 ttatgaaagg cttttagtta ttagaaaatt ttgtaaataa agaaaaacta gatgacatca
     2821 tgagataaaa ccgatagaat aagtaataat tcattttgaa agaacaaaat attgtggatg
     2881 attaaaatga gtgtagggta aaagggagat agtaaaaaat gagtgaaaaa caacaaattc
     2941 tcgattatat agaaacaaat aaatatagtt atatcgaaat cagtcataga attcatgaac
     3001 gtcctgaact tggtaatgaa gaaatatttg cgtctcgaac tttaatagat cgtttgaaag
     3061 agcatgattt tgaaattgaa accgagattg ctgggcatgc aactgggttt atagcgacat
     3121 atgattcagg acttgacggg ccagctatag gttttttagc agaatacgat gctttgccag
     3181 gattgggtca tgcttgtggt cataatatca ttggaactgc aagtgttctt ggtgcaattg
     3241 gtttgaagca agtgattgac caaattggtg gtaaagtagt cgttcttgga tgtccagctg
     3301 aagaaggtgg ggaaaatggt agcgctaaag cttcttatgt caaggctggt gtgattgatc
     3361 aaatagacat tgccttaatg attcatccgg gaaatgaaac ttataaaacg attgatactt
     3421 tggcagtgga tgttttagat gttaaatttt acggaaaaag tgctcatgcc tctgaaaatg
     3481 cagatgaagc gttaaatgca ttagacgcta tgattagtta ttttaatggt gtagcacaac
     3541 tacgacaaca tattaaaaaa gatcaacgtg tgcatggtgt gattttagat ggcgggaaag
     3601 cagctaatat tattccagac tatacacatg ctcgttttta tactagagca atgacgcgta
     3661 aagaattgga tatattaaca gaaaaagtaa atcaaatcgc acgtggagct gcgatacaga
     3721 ctggttgtga ttatgaattt ggtcgaattc aaaacggtgt gaatgaattc attaaaacgc
     3781 cgaaattaga tgatttattt gctaaatatg ctgaagaagt tggtgaagca gttattgatg
     3841 atgattttgg ttatggctct acggatacag ggaacgtaag tcatgttgtg ccaacaatac
     3901 atcctcatat taaaatagga tcacgtaatt tagtaggaca tacgcataga tttagagaag
     3961 cggctgcgag tgtacatggt gatgaagcat taattaaagg cgctaaaata atggcgttga
     4021 tggggttaga gttaattaca aatcaagacg tttatcaaga cattattgaa gagcatgcgc
     4081 atttgaaagg gaatgggaag taatgacaaa taatggtaat gagccgaact taacgttaag
     4141 tgatttatat gacaaagatg ttgtctatac gtcaagacct tcgtatatat cgaatccatg
     4201 gttgaaaccg gacgaacatc aatctaattt tttaactggt agggagttat taatagcaaa
     4261 tcaattacct gttattgttc acgaagctag tgcgacagac aaattgcatc agctatttca
     4321 agttattggt aaggaagtac cgaactcaat ttatacattt aacaatcagc aatcttacga
     4381 aaacttgatc aaacaattgg cacataagga aaataaaaag atttattttc aatatattca
     4441 tgatgaaaca atcttgaatc agcaatatta tgcattagat aaaacgttat ttgttgcttt
     4501 aaataataaa gcaagaatac ctgaatggac aaatgggaag tttttaccaa aaagaaaagt
     4561 tgtaaaaatt gagcaattcg aaaacgagat taagaattgg gaattcccgt tagtaattaa
     4621 gccgggagat gatcttccga cagcaggtgg ctatggcgtt atgatttgtt atcatgatgc
     4681 agatttacaa aaagcgataa cgcgtattaa agaggcaact gctgaaacga attcgcttat
     4741 tatcgaacag aagattgaag aaaaagcgaa ttattgcgtt cagtttgcat actcggaatc
     4801 gctaggcatt caatatcttg gtgcggcaac gcaattaacg gataagtacg gattttataa
     4861 cggaaatgaa aatactacta atgtaccaga gcacgttata gaagcaggta gacaaattat
     4921 ggaaaacggt gtgaatcaag gattctttgg tgtagctggt tttgacctac tcgtcgatga
     4981 ggatgataac gtttatgcga ttgatttaaa ctttagacaa aatggttcaa cgagcatgtt
     5041 attacttgct aacgagttga attcaggata tcaaaagttt tatagttatc attcaaaagg
     5101 tgataacaca catttcttca atacgatttt gaaatatgtc aaagaaggta gtttataccc
     5161 gttatcttat tatgatggtg attggtacgg tgaagataaa gttaaatcaa ggtttggctg
     5221 tatttggcat ggtgattcaa aagaaacagt actggagaat gaacgcgcat ttttagctga
     5281 acttgaacac tattagagtt cggaacataa ggcgctacaa tgttgtgttg ccagtagttg
     5341 actgaatatg cgtttgtaac aagctttttt cgattctagt caacagtaat taaatttatg
     5401 atatggcaat actttgtaat actaatatta aatggcgact tttatttcac tatgttataa
     5461 gagttgccat tttgttgata aaggtatact aaaggttatc gttttgaaat ttttagtaac
     5521 tagatatgtt tcgtgttata gaccgaattt gtgtatacgt aaaatttaat gctattgaat
     5581 ttttaaaatg aaaaacatga cattaaattg aattcataat atgtctaatt gactaacttg
     5641 ttggagtcat ttactatttt atgtatgaca tattttaaaa agtgagggtc aagcatgtct
     5701 tataaagcat atccattctt tagagatata ttaataaatg aatgtattta tttcgcctct
     5761 aaaaataaaa aactagtacg cctaaattat aaaagtgaag cgtatgtagg cgtttggaca
     5821 gaagaaagtg tggccgtatc atttttaaca agtcgtgata ttccatttga taaagttgta
     5881 aaaatggacg ttgatcgttt tgctacttat gaattagatg aattgtttga tgaacaagac
     5941 catattatta tgaatcaaac aatggaagaa gaagggcatc tactaaacgt tgtagctgtt
     6001 acacaagaag tgatgacgga attagataaa attagaatca aagaatttgt ccaagatgta
     6061 gcgaaatatg atgaagtata cggcttaact aaaaaaggta gtaagcagtt tattctcatt
     6121 agtgaaaatg atagcgacga aaaaaagccg catattatgc ctgtatggag tattaaaaac
     6181 agagcgttaa aagttcgaga tgaagatttt gaagagtgtg atttaattac gattgaaggt
     6241 tctgttttcg gagaatggct agatgaactt agagatgatc ataaagccgt tgcgatagat
     6301 ttaaaaactg gcgtggttgg tacaattgtt tcagcgcaaa aattgtctaa tgaacttaca
     6361 ttttaattga attgagttgt aagtaattaa gtgataataa atggaacaat acgtattcaa
     6421 aacacttaga ccataaaata aaaggccatt tatatagcgt ttatttaaaa caacgcgcat
     6481 ataaatggtc tttttctatt tttctaaata taatgcacca atagcacctg aaaatgcgcc
     6541 gttttcaaca tagtacggtt tgcaaccgcg taacacagta taatcttcca caactttgcg
     6601 taataaagcg ttattatgaa atgaagaacc gatataaacg atattttcag ttttaaattc
     6661 acgtgcaaca gtaatggcca ttgtcgtaac aacttcgcca acgacaccaa taacggctgc
     6721 taatttattg ctaggtgtaa aatcagcatc taaatgatgt agtacatgac caaaattagc
     6781 tgctgttaaa tcaccgggaa tgggtggttc ggtatcttta taaatatgtc taacctttaa
     6841 atcgatagtg ttacgatcac cgtgttgtgc catgtcagtt aactgtttat aatcagtgat
     6901 ttgacttagt aaataaccga gtccttgaat catgcctcca cctgtaccga taccgcctac
     6961 acgacgttgt gattggccgt cgaaataatg tagtgacgta ccggtaccaa catttgcaaa
     7021 aatataatct gctaagtcat ggccttgctc ttttaacaaa atacctagtc cttgagatgc
     7081 agcatcaaac tctacaaaaa tttgtgcagg aatgttgatg ttttcagcaa tgacacctgc
     7141 attacctcca gttaagcata atttttcaat ttgctgttgg tttaaccatt ccacaacttg
     7201 atcaatattt ttagttaatt cagttttaaa agtacgttgg ttatcttgct cttgaacgat
     7261 tttaattagt gtaccgccag cgtcaatgcc aactttcata agattcccac ctcattatta
     7321 atgtctatcc ttaaataata gtatagtaaa atgactaaaa aacaagtaat aatagtaatt
     7381 attaacaaat ttgatgccat tgcatttcaa cattgtaagc gtatcgcaat taatgtttta
     7441 caaacgtgga cgttaagtta tatatattat tttctaggaa ttttgaagtt gtataggatt
     7501 gttagttagt gacgcaatat taaaagtagt tcgtacgcag tgtatttgta agtctctgat
     7561 taaaatgata agtaatgagg aatagtacat taattttgaa at
//
LOCUS       DALAAR010000105         7448 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000105 DALAAR010000000
VERSION     DALAAR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.108"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            33..512
                     /locus_tag="O2458_002494"
     CDS             33..512
                     /locus_tag="O2458_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ3888337.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            527..1366
                     /gene="fakB2"
                     /locus_tag="O2458_002495"
     CDS             527..1366
                     /gene="fakB2"
                     /locus_tag="O2458_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ3888338.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            1452..1985
                     /gene="msrA"
                     /locus_tag="O2458_002496"
     CDS             1452..1985
                     /gene="msrA"
                     /locus_tag="O2458_002496"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ3888339.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            1978..2406
                     /gene="msrB"
                     /locus_tag="O2458_002497"
     CDS             1978..2406
                     /gene="msrB"
                     /locus_tag="O2458_002497"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ3888340.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            2418..2918
                     /locus_tag="O2458_002498"
     CDS             2418..2918
                     /locus_tag="O2458_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ3888341.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            2918..3139
                     /locus_tag="O2458_002499"
     CDS             2918..3139
                     /locus_tag="O2458_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ3888342.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            3331..4821
                     /locus_tag="O2458_002500"
     CDS             3331..4821
                     /locus_tag="O2458_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCZ3888343.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            5221..5730
                     /locus_tag="O2458_002501"
     CDS             5221..5730
                     /locus_tag="O2458_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888344.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            5742..6812
                     /locus_tag="O2458_002502"
     CDS             5742..6812
                     /locus_tag="O2458_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ3888345.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            6829..7443
                     /locus_tag="O2458_002503"
     CDS             6829..7443
                     /locus_tag="O2458_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ3888346.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         2781 a         1005 c         1352 g         2310 t
ORIGIN      
        1 aaaaatgaaa tgatagaaga aggaggataa ttatgacttt atccattcta gttgcacatg
       61 acttgcaacg agtaattggt tttgaaaatc aattaccttg gcacctacca aatgatttga
      121 agcatgttaa aaaattatca acaggtcata ctttagtaat gggtcgtaag acatttgaat
      181 cgattggtaa accactaccg aatcgtcgaa atgttgtact tacttcagat acaagtttca
      241 acgtagaggg cgttgatgta attcactcta ttgaagatat ttaccaacta ccgggccatg
      301 ttttcatatt tggagggcaa acattatttg aagaaatgat tgataaagtg gacgacatgt
      361 atattactgt tattgaaggt aaattccgtg gtgatacgtt ctttccacct tatacatttg
      421 aagactggga agttgcctct tcagttgaag gtaaactaga tgagaaaaat acaattccac
      481 atacctttct acatttaatt cgtaaaaaat aagggggaaa acgaccatga caaaacagat
      541 tatagtaaca gactcaacat ccgatttatc taaagaatac ttagaagcaa acaacattca
      601 tgtaattcct ttaagtttaa ctattgaagg agcttcatac gttgaccaag tagatattac
      661 atcagaagaa tttattaatc atattgaaaa tgatgaagat gtaaagacaa gtcagccagc
      721 cataggtgaa tttatatctg cttatgaaga actaggaaaa gatggctctg aaatcataag
      781 tattcatctt tcttcaggat taagtggtac atataacact gcttaccaag caagtcaaat
      841 ggtagatgct aatgtaactg ttattgattc aaaatctatt tcttttggtt tagggtatca
      901 aatacaacac ctagtagagc ttgtaaaaga aggtgtctca acttctgaaa tagttaaaaa
      961 gttaaatcat ttaagagaaa acattaaatt atttgtagtt atagggcaat tgaatcaatt
     1021 aattaaaggt ggcagaatta gtaaaacaaa aggtttgatt ggtaatctta tgaaaattaa
     1081 accaattggt acactagatg atggtcgctt agagcttgtg cacaatgcga gaactcaaaa
     1141 ttctagtatc caatacttga aaaaggaaat tgctgaattt ataggagatc atgaaatcaa
     1201 atccattggt gtcgcacatg ctaacgtcat tgaatatgtt gataaattga agaaagtttt
     1261 taatgaagct tttcatgtga ataattacga tataaatgta actacaccag ttatttctgc
     1321 acatactggt caaggtgcga ttggcctcgt agtccttaag aagtaaattt aatcttttca
     1381 gtgttaatta cttccatttc aatcctttat agactaaatt tataattaga tagatagagg
     1441 aggtaattca tatgacaaaa gaatatgcaa cattagcagg aggatgtttc tggtgcatgg
     1501 ttaaaccatt tacatcatat ccaggcatca agtcagtcgt atctggttat agtggcggtc
     1561 atgttgacaa cccaacttat gaacaggtat gtacgaatca aaccggccat gtcgaagcag
     1621 tacaaattac gtttgatcca gaggttactt cctttgaaaa tatattagac atatatttca
     1681 aaacatttga cccaactgat gatcaagggc aatttttcga tagaggcgaa agctatcaac
     1741 cagtcatttt ctatcatgat gaacatcaga aaaaggctgc tgagtttaaa aagcaacaat
     1801 taaatgaaca aggtattttc aagaaaccag tgattacacc tattaaacca tataaaaatt
     1861 tctatccagc tgaagactac catcaagatt attacaaaaa gaacccggta cattattacc
     1921 aatatcaacg tggttcaggt agaaaagcgt ttatagaatc acattggggg aatcaaaatg
     1981 cttaaaaaag ataaaagtga actaacagat atagaatata ttgttacaca agaaaacggc
     2041 actgaaccac catttatgaa tgaatattgg aatcattttg ctaaaggaat ttatgtagat
     2101 aaaatttctg gtaaaccttt atttacatct gaagaaaagt ttcattctga atgtggatgg
     2161 cctagctttt ccaaagcgct tgatgacgat gaaattatag aattagtcga caaatcattt
     2221 ggtatgttga gaactgaagt gcgttcagaa gaatcaaata gtcatttagg acatgtcttt
     2281 aatgatggac ctaaagaaag tggcggctta agatactgta tcaattccgc tgcaattcaa
     2341 tttattccat atgaaaagtt agaagaattg ggttatggcg atttaatatc acattttgat
     2401 aagtaggagt ggaaagtatg tttaaaaaat tattcggaaa aggcaaagaa gttcagaaag
     2461 atatcgcaat ttatgcacca ttaactggag aattcgtgaa aattgaagat attccagatc
     2521 ctgtattcgc acaaaaaatg atgggcgaag gttttggtat taatccaact gaaggagaag
     2581 ttgtgtctcc aattgcagga cgtgttgaca atgtctttcc aactaagcat gcgattgggt
     2641 taaaagcaga taacggatta gaattattag ttcatatcgg tttagacaca gttcaattag
     2701 acggtgaagg atttgaagtg ttagtgtcta gtggtgacga agttaacgtc ggagatccat
     2761 tagtaagatt caaccttgaa tatattaata ataacgctaa atctgtaatt tcaccaatta
     2821 taattactaa cactgatcaa gcagcttcaa ttaatattta tgatgaaaat gctgtgatta
     2881 aaggtgaaac aaaagtgatt gatgtgacaa tgaactaatg aaaaactatt cgttttatca
     2941 atttgtcatg acagttcgtg gtcgacacga cgataaaggt cgtctagcag aagagatatt
     3001 tgacgatctt gctttcccaa aacacgatga tgattttaac atactgtctg attatattga
     3061 gacacatggt gatttcacat tgccaatgtc tgtatttgat gatttatatg aagaatatac
     3121 ggaatggcta aaattttaat ataattttta ataataatag ttagaaccag ggtgatgcaa
     3181 ttcgttatcc tggttttaat ttaaaataaa ctaagttgtg actaaaaatt aatcaattat
     3241 agtgaaatat ggtgcgctat cttgcataaa ttgatatgat taactacaca gaatttaaaa
     3301 gtacataata cataataagg aagtgataca atggatgata agcaacacac atcttcatcc
     3361 gatgatgaac gcgctgaaat tgcaacaagc aatcaagacc aagaaactaa ttcatcgaaa
     3421 cgcgttcact taaaacgttg gcaattcata tcaatattaa ttggtacaat cctaataaca
     3481 gctgtcatca cagttgttgc atatattttt ataaatcaaa aaataagtgg tttaaacaaa
     3541 actgatcaag caaacttaaa taaaattgaa aatgtgtata aaatcttaaa tagtgattat
     3601 tacaaaaaac aggactctga caagttaagt aaagctgcaa ttgatggcat ggtcaaagaa
     3661 ttaaaagatc cttattctga atatttaaca aaagaacaaa cgaaatcctt taatgaaggt
     3721 gtttcaggtg attttgtagg tattggtgca gaaatgcaaa agaaaaatga tcaaattatg
     3781 gttactagtc ctatgaaggg atctccagca gaacgtgctg gcattcgtcc taaagatgtc
     3841 attactaaag taaatggaaa atcaattaaa ggtaaagcat tagatgaagt tgtcaaagat
     3901 gttcgtggta aagaaaacac tgaagtcact ttaactgttc aacgaggtag tgaagaaaaa
     3961 gacgttaaga ttaaacgtga aaaaattcat gttaaaagtg ttgagtataa gaaaaaaggt
     4021 aaagttggag ttattactat taataaattc cagaatgata catcaggtga attgaaagat
     4081 gcagttctaa aagctcacaa agatggtttg aaaaagattg ttttagattt aagaaataat
     4141 ccaggtggac tactagatga agctgttaaa atggcaaata tttttatcga taaaggaaaa
     4201 actgttgtta aactagaaaa aggtaaagat actgaagcaa ttcaaacttc taatgatgcg
     4261 ttaaaagaag cgaaagacat ggatatatcc atcttagtga atgaaggttc agctagcgct
     4321 tctgaagtgt ttactggtgc gctaaaagac tataataaag ctaaagttta tgggtcaaaa
     4381 acattcggca aaggtgtcgt acaaactaca agagagttta aggatggttc attgttaaaa
     4441 tatactgaaa tgaaatggtt aacgccagat ggtcattata ttcacggtaa aggcattaaa
     4501 ccagacgtta ctattgacac acctaaatat caatctttaa atgtcattcc taatacgaaa
     4561 acatttaaag ttggagacga tgataaaaat attaaaacta ttaaaattgg tttatcagct
     4621 ttaggttata aagttgataa tgaatcaacg caatttgata aagctttaga aaatcaagtt
     4681 aaagctttcc aacaagcgaa taaacttgag gtaactggtg aatttaataa agaaacgaat
     4741 aataaattta ctgagttatt agttgaaaaa gctaataaac atgatgatgt tctcgataag
     4801 ttgattaata ttttaaaata agcgatacac actactaaaa ttgtattatt attatgttaa
     4861 tgacacgcct cctaaatttg caaagatagc aatttaggag gcgtgtttat ttttattgac
     4921 gtctaactct aaaagatata aattagacat ttacaaatga tgtaaataac gcaatttcta
     4981 tcatcgctga taacaattca tggtttaata tgcaatgagc atatactttt taaatagtat
     5041 tattcactag ttttaacaat caattaattg gtatatgata cttttattgg ttatttttat
     5101 cccatagtgt gataattact atttttcatt cataataaag gtttaaagca tgttaatagt
     5161 gtgtaagatt aacatgtact gaaaaacatg tttaacataa tgatataagg agtgacgtac
     5221 atgatccgtc taggtaaaat gtcagattta gatcaaatct taaatctagt agaagaagca
     5281 aaagaattaa tgaaagaaca cgacaacgag caatgggacg atcagtaccc acttttagaa
     5341 cattttgaag aagatattgc taaagattat ttgtacgtat tagaggaaaa tgacaaaatt
     5401 tatggcttta ttgttgtcga ccaagaccaa gcagaatggt atgatgacat tgactggcca
     5461 gtaaatagag aaggcgcctt tgttattcat cgattaactg gttcgaaaga atataaagga
     5521 gctgctacag aattattcaa ttatgttatt gatgtagtta aagcacgtgg tgcagaagtt
     5581 attttaacgg acacctttgc gttaaacaaa cctgcacaag gtttatttgc caaatttgga
     5641 tttcataagg tcggtgaaca attaatggaa tatccgccat atgataaagg tgaaccattt
     5701 tatgcatatt ataaaaattt aaaagaatag aggtaatatt aatgacgaaa atcgcattta
     5761 ccggaggggg aacagttgga cacgtatcag taaatttaag tttaattcca actgcattat
     5821 cacaaggtta tgaagcgctt tatattggtt ctaaaaatgg tattgaaaga gaaatgattg
     5881 aatcacaact accagaaatt aagtattatc ctatttcgag tggtaaatta agaagatata
     5941 tttctttaga aaatgccaaa gacgtattta aagtattgaa aggtattctt gatgctcgta
     6001 aagttttgaa aaaagaaaaa cctgatctat tattttcaaa aggtggattt gtatctgtgc
     6061 ctgttgttat tgcagccaaa tcattaaata taccaactct tattcatgaa tctgacttaa
     6121 caccaggatt agcgaataag atagcactta aatttgccaa gaaaatatat acaacatttg
     6181 aagaaacgct aaactactta cctaaagaga aagctgattt tattggagca acaattcgag
     6241 aagatttaaa aaatggtaat gcacataatg gttatcaatt aacaggcttt aatgaaaata
     6301 aaaaagtttt actcgttatg ggtggaagct taggaagtaa aaaattaaat agcattattc
     6361 gcgaaaactt agatgcatta ttacaacaat atcaagtgat acatttaact ggtaaaggat
     6421 taaaagatgc tcaagttaaa aaatcaggat atatacaata tgaatttgtt aaagaggatt
     6481 taacagattt attagcaatt acggatacag taataagtag agctggatca aatgcgattt
     6541 atgagttctt aacattacgt ataccaatgt tattagtacc attaggttta gatcaatccc
     6601 gaggcgacca aattgacaat gcaaatcatt ttgctgataa aggatatgct aaagcgattg
     6661 atgaagaaca attaacagca caaattttat tacaagaact aaatgaaatg gaacaggaaa
     6721 gaactcgaat tatcaataat atgaaatcgt atgaacaaag ttatacgaaa gaagctttat
     6781 ttgataagat gattaaagac gcattgaatt aatggggggt aatgctttat gagtcaatgg
     6841 aaacgtatct ctttgctcat cgtttttaca ttggtttttg gaattatcgc gtttttccac
     6901 gaatcaagac ttgggaaatg gattgataat gaagtttatg agtttgtata ttcatcagag
     6961 agctttatta cgacatctat catgcttggg gctactaaag taggtgaagt ctgggcaatg
     7021 ttatgtattt cattacttct tgtggcatat ctcatgttaa agcgccacaa aattgaagca
     7081 ttattttttg cattaacaat ggcattatct ggaattttga atccagcatt aaaaaatata
     7141 ttcgatagag aaagacctac attgctgcgt ttaattgata taacaggatt tagttttcct
     7201 agcggtcatg ctatgggatc aactgcatat tttggaagtg gtatctatct attaaatcga
     7261 ttaaatcaag gtaattcaaa aggtattctt atagggttat gtgcagctat gattttattg
     7321 atttccatat cacgtgtata tctaggtgta cattatccaa cagatattat tgccggcatt
     7381 attggtggat tattttgcat tattttatca acgttattac ttagaaataa attaataaat
     7441 taaatagt
//
LOCUS       DALAAR010000106         7203 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000106 DALAAR010000000
VERSION     DALAAR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.109"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(76..1059)
                     /locus_tag="O2458_002504"
     CDS             complement(76..1059)
                     /locus_tag="O2458_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCZ3888347.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            1485..2228
                     /locus_tag="O2458_002505"
     CDS             1485..2228
                     /locus_tag="O2458_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ3888348.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(2455..2640)
                     /locus_tag="O2458_002506"
     CDS             complement(2455..2640)
                     /locus_tag="O2458_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888349.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(2645..2983)
                     /locus_tag="O2458_002507"
     CDS             complement(2645..2983)
                     /locus_tag="O2458_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888350.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(3229..3837)
                     /locus_tag="O2458_002508"
     CDS             complement(3229..3837)
                     /locus_tag="O2458_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888351.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(4323..5222)
                     /locus_tag="O2458_002509"
     CDS             complement(4323..5222)
                     /locus_tag="O2458_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ3888352.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(5321..6787)
                     /gene="msr(A)"
                     /locus_tag="O2458_002510"
     CDS             complement(5321..6787)
                     /gene="msr(A)"
                     /locus_tag="O2458_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ3888353.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         2305 a         1105 c         1026 g         2767 t
ORIGIN      
        1 tcgtcatcag ttaaaaagtc agatgattta acggaatatt ttgatgtctc ttcgagtgtt
       61 ataaaatcat ttgaattatt gattaaagat attatattgt ttcatgtttt tacggcgcat
      121 ataaagggca tgtgtttctt caagttcagt tagaattgtt tgcattaaat aagcttgcat
      181 tgacttgatt gtttcctttt ggcctttaac tcccctatca tgtatagctt ttttaagtct
      241 ttttaaagaa gttaatattt catcctcaat gtcctctaac atgtaatgca tatcttcatg
      301 ccttgaatta aatgattttt ttgctttaag aataacagcc tttatgattt ctaaatctct
      361 ataatcatat tttaataaat aattagttag ttgttctggg aattcttgca atagcatttc
      421 tttatcatca aaattatgtg taaattgatt tgaatgattt gaatgattac taatttcatt
      481 tttgtacttt atatcattca agtcattcat atcattattc tcagtatcat taaagtcaat
      541 gtcattaaag tcagtatcat tagtgttcga ttttcgaact tctggacgtt cgattttcga
      601 acttctggac gttcgatttt cgaacttctg gacgttcgat tttcgaactt ccttctcgcc
      661 gtaaggttca acatcgtttt cttctttttc tattttgtaa atatcacgtt ctgtgacaat
      721 aggtttcatt aaatatagaa tatttggttt atttaaacca cgtcgttttt gaatgagtag
      781 accatcgttt tctaaccctt tttttatttt tgttaacttt tcttttttac aatttaagat
      841 ttccattagc ttttcatttg aataaacaaa gtaaatgtta ccatcttcat caacccagct
      901 atttttaata gataaattta agcgatcacg taagatagca taggctattt tcatatcatt
      961 agttagattt ttataatttt cactggtaaa gaatacttta gggatttgat aaaatctttc
     1021 tttgtaattt tcttgcactg taaaatattg attttgcatg acaaaaaacc tctttccata
     1081 ttttagtttg gtaagaggta ataaaacgtt tttatttagc tttacattta tttaatgatt
     1141 taggtgcttg cactaagata ttattcatta tataatacaa gtatcgaaaa ttatataaat
     1201 aaacagtttc taaacgtttt attaccaaac cccgactatt accagtagtt ggggtttttg
     1261 ttatattaag tttattatag cactttaaat aaacttataa aagggaaaat atagctttat
     1321 caagggttta taaacccttg ataaatatca taaacctttc ataaaccctt gataaaaagg
     1381 ttgatggatg tttattttta tttattaatc actttattat taagcattat aagcttataa
     1441 tattttatca agggtttata aacattttat aaagggttga tgatatgaaa agtgtgaaaa
     1501 agttgagtga agaattaggg gttagtaagc aaacgatttt taataatatt aagcgattga
     1561 atatagagac aataaaacag gaaaacactt cgtttattaa agaagatacc gatatagaaa
     1621 aaatcattca aagagtaaat gaaaataaaa agaaatatgg ttttgaaagt actactgaag
     1681 acaaacaaaa aaaagggtcg gataatatta attttgaatc gaaaagcgat gctcaaatag
     1741 tagaaatcct taaaaatcag attgatactt taaataatca aattgaaaaa caagaaagtc
     1801 gtcatgaaac aacaattgaa ttttacagaa aagagttgca agaaagatca aaattgcttg
     1861 aaaatcagca agttctggca ttagagagta ataaaaagat tcaaaaatta gaaaatcaat
     1921 tagaaaaaga aaaacacctt aattatccct ttgatacatc tactaacact agacaaaacg
     1981 ttgatgcaca agaaaaaaca tatactacta atcctgtaaa tattaacaga gatcaggaag
     2041 aaactaaaga aacagagatt caatataagg atatatctgg aagtcaaagc gacgaaagta
     2101 cacaaggtga agaagcaaaa aaagaagacg ttccagctaa tctaaatgac aatgatagtg
     2161 atatagaaga aaagagtgaa gaaacggaac ctaaaaaggg gttttggagt cgtttgtttg
     2221 gtagctaaaa taattgatta ggcactaaaa gttataaaat tttagtgtct tttatatagt
     2281 gagaacttct ggtaatcatc gattatgtaa actaaagtat ttcaaataat taactgtatt
     2341 aatactctca tcatgattgc gttataaacg ttcgttattg agacaaaatt taatagtact
     2401 actacacaaa aagagagtgc gaattcgcac tctctttttg tgataaatga atggttaaga
     2461 aaataattca tccacagttg tatcaaacaa tcttgccaac ttaaatgcta atgtcaatgt
     2521 aggatcatat ttattgtttt ctattgcatt aatagtttgc ctagacactt ttaattcttt
     2581 acttaaagtt tcttgagaaa ttcctttttg ttttctatat tctctaattc tatttttcat
     2641 taatttaacc tttagatgta tattttttat ttgtagtata gtaattaaac agcataagaa
     2701 atagtgctac aattataaag aaaatttctg gatattgaga aaaccaagat ccttcaatac
     2761 ttggtgacaa acctgtaaat ttaagcacta gtgtaaataa catacaatat acaatccaaa
     2821 taaaactttt agccagtatt tcaataaaca ttttacgttg tctttcatcg tatcgatctt
     2881 gttgtccttt tttatctaat gtttctgaag gtaaatattt gttagttata gccgtccata
     2941 aacaagaaaa aattaaaata ataataaaag gaattaaatt cattataaca cctcataaat
     3001 gtaaaaagta tttgacattt atgagtgtaa atatttttga tacaaatgtc aaatactttt
     3061 tacattataa tatattttaa aacaccttat tagtaaaaca attactatat agaaagctgc
     3121 gaactcgtac tcttttttct tgtataatta ttaaaatctc aattcaataa tatgttatgg
     3181 aatttgagac actgtgagag acgctcattt gcattgacaa tcaatttatc aagataatcc
     3241 cttatcatgt ttaattctat aaactgtttg tcttgtgata ttaacttctt tcgcaatctt
     3301 actaatcgct ttgccttctt ctaacatttc caccacgcga tgataaataa cacgtttctg
     3361 tgggtcttta gcatttggtg aataaagtaa agggcgccct ttatatacgc ctttttcctt
     3421 cgcaacttga atcccttgcg cttgtcgacg tttactttca tttctctctt gttctgaaac
     3481 cattgctaat atttgaatga ttaaatcttt cataaactta tccagtaatg gattgccaat
     3541 tacttcattc atcataggta agctggtaat cattaactga acgtcttttt cttttaaata
     3601 attcacagtt tcaataattt catcatagtt acgtcctaaa cgatcaatag attccacaac
     3661 gaatcgatct cccattctga caaaattgag tgcttcttga aatacaggtc tattttccac
     3721 tgactttccc gactgtttct ctgtaaagat tttctccaca ccaaacgtct ttaaattatc
     3781 caattgtctt tctaaatttt gatctatcga tgatactctc gcataaccta taatcattca
     3841 tcttcactcc ataataaata tacaccctaa tcatacgttt taattgttat aatttcaaca
     3901 attctttttg tatggttagg gtgtactcaa agtgtacata tctataatta aaacttacta
     3961 aaaataaata tagatttttt ctttgtaaca gaaccgttca tttgaaggtc atctctatca
     4021 taaatattta aattaactaa aaattttaat catctgttta atacttatga aatgtattta
     4081 cggtaggtat taaaattgat gaaatttata taagtgcttt caatgaaaat gaccgtttat
     4141 ttatcctcct gatttaagga gttagaaatt tcgaaaacga ttctttccat ttgttgcatt
     4201 tgttccatgt ccagatattt aatacattat agaaaaccaa agaccatcac taattaaacg
     4261 cagttttaaa atttcatcag gattgccttc gtctttaaaa tgggcctctt ttaatataag
     4321 ttctactctt tcatacctaa ctctttcaat gccatcgttt catacgccga ttctcctgat
     4381 tccatagcaa actcagcgat aaacattggg aatactgcat tgagttctat tatatgctct
     4441 ttcattcgtg gccatatttc acctccagct ttaccgtatg ctgttataag ttgctctaat
     4501 ccttcgtcgt cgaatacacg atggtgtcct ataaagtcca ttgatgggtc ggagtgggtt
     4561 gcttcagtcc agtctatgag acctgtgacg tttgcttggt tatctaccat tatatgtcct
     4621 ggatgtaagt ccccatgtat catggtcgca tgtcgaggcc atagttcgtc gttttctaac
     4681 cattgtttcc atctgttcca taattcatct gatacgccat aagtttcttt aactttattc
     4741 attcttcttt gaaagtcatt ttttatttct tgtatggttt tgatatttat atgctgaacg
     4801 ttaatgtttt cttctggtat gttgtgtaaa tctacgagtg tttcagctaa tgtgttaata
     4861 aagttttctg gtaatggttt gtgttcaatt tcccatacat aattttgtat ttctggatct
     4921 attgtggctg cgggtttacc tgtaagtttt gggtaagcaa taaggtcttt tgcatgtact
     4981 ttccacttcg gtatttcaaa tgaaacattc ttctgtaaga agtctaccgt ttgtttttcg
     5041 ggttttgttc gtttataaac gtcaggtctt cttggtagtc tcaaaaccca ttctactcca
     5101 tgtttatctt ttccaaatgc aacttgaaaa tcaagtcccg attcattcaa tgagattgtt
     5161 tgaggttgga tgtgtaattg atatttttct gcacatttaa taatttcatt atgtcgagtc
     5221 attttattgg atccttattt ctattatatt tttcttgtga acgtattttt tatattgata
     5281 taaatgaacc taatattcaa tataaattag tgtatagttt ttaagttata tcatgaagag
     5341 attgtcctgt taattcccat tttttatctg atacatgttt aacaaacctt gtatcatgtg
     5401 atgtaaacaa aatgattcca ggatatttat tcataaacat ttctaatgct tctaatgttt
     5461 taatatctaa aaaattagtt ggttcatcca aaattaacat attcgctttc gttgaaaata
     5521 atactgctaa cgataatttc gttctttccc caccactcaa aacattacaa gaacgatcaa
     5581 gtgcttcatt taaacccaag ttatttaaaa tagctctact gaatgattct gatgaatccg
     5641 tttcatccat taaatattgc aataatgaaa cgtcacgcat gtcttcataa gcaagttgac
     5701 gatagtatgc catttgcact ttaggagaac aatcaattcc ctctatttgg tggtaaatag
     5761 cttcaagtaa agttgtctta cctacaccat ttgcacctac gagcgctata tttttgccat
     5821 atggtatttg gaatcgtact tgtgttagca gtttttgact tcctttaacc aatgttagat
     5881 tttgtgcaat gattggataa ttattatgga tatcataaat tttattttgt gggaaattga
     5941 attcatgata actttgtggt ttttcaactt cttccaaatg ttccattctt ttttcaatat
     6001 gcttagcttg tttttgagca gccttctcaa ccgtgccttt ttgttttgat gcacttaaac
     6061 gatctggtgc tatacttttg tttttttgtt ttgatgatgc ttgtgccatt tgttgcgctt
     6121 gatttcgttt agctttactg gcttgggaca atctttgttt ttcacttata tactgttcat
     6181 attgacgttg ttgttctaac tgttcttgtt catattgatt ttgatactgt gtgtaattac
     6241 ctttaaacac tcttattgtg ccatcttctt gtatatccca aattgtgtca gcaatttggt
     6301 ctataagtgc tctatcatga cttactataa ttaaagtacc atagtaatat tttaaaatat
     6361 ttttcagata atctttacca attttatcca agtgatttgt aggttcatct aaaagtaata
     6421 tcggactata atttgatatg acattagcta atttatattt tgctttttca ccaccactca
     6481 tactgtcggt tgtatgcata ggtatatgga gttcactcat taaactaccg tctaacgtgt
     6541 taaaatcatt ttccacatcc atgttaagct gttcaaaata ttgaatttcg ccaaccgttt
     6601 caactgttcc agattctggt tttgtttttt gagcaatcat atttagtaat gtggttttgc
     6661 cagtaccatt acccccaata agtgctattt tttcaaattg gtaagcgtaa agatgaccga
     6721 tgttaagtga tcgtaaatct gtcaatttat gattgatttg gttaaattta attgtatatt
     6781 gttccatatt tatacactcc ttgtatattt aaaggacgtg tacgcaaaaa aatgagacag
     6841 gctactcccg tctcattcac attacacttt caaacactta aaaatttatt atgatgtttt
     6901 agtatttgaa agcgtttatt attcctatcg aaggataaaa aatgacataa gtcatcctat
     6961 gagtgtgtat tgcggacaag cctttttgaa ttaatttagt tttgtattca aaaaggttta
     7021 tttgagtctc atagaagctg tcattttaat cctccttcat cgataaaata gtaatgttaa
     7081 tataacattt tgttggtttt tttacaatat atctctaagt agaaagatta tataacaaaa
     7141 gtataaaaat acaattttag attgattata catctcctta atttagagtg tactcataaa
     7201 ata
//
LOCUS       DALAAR010000107         7132 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000107 DALAAR010000000
VERSION     DALAAR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.110"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..347
                     /locus_tag="O2458_002511"
     CDS             <1..347
                     /locus_tag="O2458_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3888354.1"
                     /translation="KVISFIKINDSNYRLSNVDTMKVTLYSNGSNYDKEALLINKDEF
                     CPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFE
                     VNYYTYNHIMHH"
     gene            781..1383
                     /locus_tag="O2458_002512"
     CDS             781..1383
                     /locus_tag="O2458_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888355.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            1650..4802
                     /locus_tag="O2458_002513"
     CDS             1650..4802
                     /locus_tag="O2458_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCZ3888356.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4859..5341
                     /locus_tag="O2458_002514"
     CDS             4859..5341
                     /locus_tag="O2458_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888357.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            5546..6532
                     /locus_tag="O2458_002515"
     CDS             5546..6532
                     /locus_tag="O2458_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCZ3888358.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            6753..>7132
                     /locus_tag="O2458_002516"
     CDS             6753..>7132
                     /locus_tag="O2458_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888359.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYP"
BASE COUNT         2745 a          853 c         1298 g         2236 t
ORIGIN      
        1 ataaggttat ttcattcata aaaattaacg actcaaatta tagacttagt aatgttgata
       61 ctatgaaagt aacgctttac agtaatggaa gtaattatga taaagaggct ttactaataa
      121 ataaagatga gttttgtcct ttaaggaaaa taactttaga taataagctt gattcacaac
      181 gggtaatgga aattgattca ttggcagcaa taataaactt agttaaacaa ggaaaaggaa
      241 aggctttatt acctatgact tttgagaata aaagagatat agtacaggac atttctaaga
      301 tatttgaagt taactactat acttataatc atattatgca tcattaattg aaagttgatt
      361 gagtttaaat tacaggaacg attttaaaag tctagtagtt agaatgagcg atgtgacaga
      421 aaagaaatta tgccttttat tttgtaaatt cgttgtttta aagtaaaggt ccttgtagta
      481 taggaaaaag tggatgactt ctccgccttt accacccttt caagcataat gataactgct
      541 aaatattgat tatagcacca ttgcaaaact aatcattaat tgtcatattc aactattttg
      601 aaaagtatca atttcacatt atcaaattat tgagtatctc aatttattgt tatacacaaa
      661 cagtattttt gttatcatag agttaataaa tataataaat taaacaagta actttacaaa
      721 aattagaatg attcaattca atcataattg tttgaaaata gaggtgagca ggtgaacgat
      781 atgttaatta gtcttgtaat cccagttttt gctttgttag ttattggtgg tattatttgg
      841 atgattatag aaggtatagt acatatttca aaaaagaata aagcaattga taactttttt
      901 aaccaagtta ataaagtaag tgagacatat aaattcgcta ctactttttt atttctaatc
      961 ttggctacgg ctggtatttc tcaattttat ctatattata tagtatcagc gtttcttttt
     1021 tggcttgtca tacttacctt tggcattgca ggtatcattt ttttaatgcc atatggatta
     1081 tgttttctac cgttttataa gcaaaaaaag aaaaaacaga catttaaaaa atacatggtt
     1141 tacactacga ttggtttgtc aatttgtcta ggcttatctc tagttttggt tcacactacg
     1201 aaaatttata tggacgaagg tggcgtaaga tactattacg gtagttttgt aatgaaacaa
     1261 gcgggcggtt atgcttattt agctttagcg gtactttcaa cgttgttaat tgttgcgaaa
     1321 aaagctacaa ataaaaataa agaaatcgaa accgtcgaca atacaaatat aacggaaaga
     1381 taattaaggg agtgctcatt caggagtgct cttttttgat gtccaaattt agttgcaaat
     1441 gaaggcatag aagaaatttt tatagtcatt tattggataa tgaattatgg tcatatcgat
     1501 taattttatt agtgaggatt ttacaaacat ttgttgaata aatatggtaa tgataatata
     1561 tgaatatttt tgaaaaaaca ctaatatgat taaattattt gttaaaatag cattaagtaa
     1621 aaaacaaata gggaacaagg tgggatttca tgagtcaatt gctaaatgat acgttatcgg
     1681 cttggttgtt aattgaatct ttaagtccag gagaagtaaa ttttacagcg gaagatatac
     1741 tctcagctga acattttaaa aatggtgcaa agcaagcaca acttcaaagt tttgatgaat
     1801 attttgaaat atggaatgct gaacgcttta ttatatcaga agaaaaatca gagactgggg
     1861 aacttatatt taaattttat agacattgct tccgctataa tgaaattaat ttgaaaattc
     1921 aggatatttt tgatgattat tctgagattc ataatccaaa tgggacacac tgttatggtt
     1981 acacatttaa cacagataaa cacggcaaag tgatagttga ttctatacat attccgatga
     2041 ttatgagtgc attaaaagaa attgaaaaga acaaaaatgc caatatagaa gaaaaattta
     2101 atgattctgt tgaaaaattt gttcaaaaag taaaagaaat tttagcagat gaaccaatta
     2161 atgaatttaa attgaagaag atggacaaag cttatgatga gtacttttct gtattaaatt
     2221 caaagaaaga tggattattt ggacattatg tagcaataga atatgtgaaa gatagtgatt
     2281 taccacagcc ggaatttaac agcttcttca taagtgatat tgagaaagca agaaaatctc
     2341 ccaaccaaac tttaattgat tacattgaag gtgtagaaga aagtcagcac atagaagtag
     2401 atgaaaataa agaaatgttt gacaaatttt tacatccttc acgtttgcct gatggacgat
     2461 ggccatcaca gactgagttt agattgtctt taatgcaaca acttgctgta aaccaaatta
     2521 cgagtggtaa tgaaagaata agttcagtta atgggccacc aggtacaggt aagactactt
     2581 tattaaaaga tatatttgct catctagtag ttgaaagagg taaagagtta gctaaactaa
     2641 ataatcctaa agatgcattt gtcaaaacaa aaattcatga aacggatgat aaatacgtat
     2701 acttactaaa ggaatctatt gccaaatata agatggtagt cgcatctagt aataatggag
     2761 ctgttgaaaa tatatctaaa gatttaccga aaattgaaga aattataaga aatcccgaaa
     2821 aatgtaaatt ccctaaatat gaacagaatt atgcaaattt agcacatgaa ttaaaagatt
     2881 ttgctgaaat agctgaagat ttgattggtg aaagtgcctg gggcttattt tctggagttt
     2941 ttggtaaaag tactaatatt aaccaagtat tgagtcatat gttaaaacaa gatgcgaatg
     3001 atattggctt tgctaaatta ctacaaaatg agaataatcg tatgagttat aacgagttaa
     3061 tgagtgaatg gcaatcacat caacgtgcat ttttagaaga gttgaggcat gttgaaatgt
     3121 taaaagaaga atctattaga gcatatgatg tttataaaaa ttgtgagtct ttctctaaga
     3181 ttgaacaggt tattaatagt gaaaaaacaa gtattgaaga acaggtatat catttagata
     3241 atgaaacgtt acgagacaat aaagaaatag aagatttgga taatcgaatt aattatattg
     3301 ttaagcaaat agaaacttta aatgagttaa ttaaatccat caaagaaagc aacaaaggtt
     3361 ttattaacaa actgaaagcg atgtttaatt cagaagaaga tgaaagctat aaagatcata
     3421 ataaagagaa gcaacaatta ttaacacaac agttagagtt agagaaatgt aaaaaaaaca
     3481 aacatgaaga ccttgttagc aaactaaaag aaaaagagaa attaattaaa caattaacta
     3541 aagtacagtt gcaattagac gagttaaatt cacagttaca agagttagaa gcatatcgta
     3601 ttgagtcaaa aattacaatt ccagaaaaag atttttggag tgacaacaat tatgatgagc
     3661 gccaagttac taatctgtgg acgagtgacg aacttcaata cagacgtgcc atgctctttt
     3721 taagagcaat gatattgcat aaattattat tgattgctaa taatacaact atttattatg
     3781 cgattaatga ttttaaagat agaaggaaat taattgatgc aaatccagat aaagtacaca
     3841 acgcatggaa tgtgatgcat ttaatatttc cagtagttag tacgacgttt gcaagcttta
     3901 aatctatgta tgggggcata ccaaaagatt tcatagacta cttatttatt gatgaagcag
     3961 gacaagcaat acctcaagca gctgtgggag cattatatcg ttcaaaaaaa gttgtagctg
     4021 taggtgatcc gattcaaata gaaccggttg tgactttaga aagtcattta attgataaca
     4081 ttcgtaaaaa ttatcatgtt ccggaatatc tagtttctaa agaagcttct gtgcagtctg
     4141 ttgcagacaa cgccaatcaa tatggttttt ggaaatctga tgctactgat agtaatcaaa
     4201 aaacctggat aggcatacct ttatgggtgc acagacgatg tttaaaacct atgttcacga
     4261 tagctaacca aatcgcttat aataataaaa tggtgttgcc aagtaatatt acaaaagtag
     4321 gtaaaacagg ttggtatgac gttaaaggaa acgcagttca aaaacaattt gtgaaagagc
     4381 atggtgaaaa agtagtggga ttattagctg atgattggat tgaagcaatt aaggaaggta
     4441 aaaatgaacc gagctcattt gtaatatcgc ctttttcagc agtacagcaa cagattaaac
     4501 gtatgttaaa gcaacaacta ccgactagaa ttgatattga acgtacaaaa attaatcaat
     4561 gggtcgataa atccattggt actgttcata cttttcaagg taaagaggct cagaaggtgt
     4621 attttgtaat aggtactgat aatacccaag atggtgctgt gaactggtca tgcgaaaaac
     4681 caaacttgtt aaacgttgca gtgacaagag ctaagaaaga gttttatgta attggcgaca
     4741 tgcaaagaat acagatgaaa ccattttatg agacgatttt taaagaaaga aatgtaaaat
     4801 aacatacaaa aaagtatata gaggaagtta tacattttaa aaggagcaaa attgaataat
     4861 ggagaaattt aacaactgga tattaaatgc aataagtgga tctcaaacag acaagaatga
     4921 aacaactgaa gaattaaaag gggcaaaatt tatcatttta tatgcatatt caatgctcgt
     4981 tttgcttgcg ttagtaattt ctaacatatt cattcacatt ttggagccta aactatcaat
     5041 caccactcaa atcatcatcg ttttgatttt aattgaagca ctaattggac tgcgtttctt
     5101 gaaagcgtac gatgttaagc gtggcaaaga taaagaaaat aagaaaaata gtaaggattt
     5161 cgttaaacta aaatcaattt tagtagcaat tttatttaca tcattggcgc tgacagcagg
     5221 tactgtagct gatatatacg gtttcactga cttaggaaat actagaagtg atttaatcgt
     5281 ttggagcata ggtggtatta tatttggcct cgtatgttac acaatggaag ataaaagata
     5341 acgataagga gctggcgatt ataaagctag ctcctttttt aacttatata tgtaaagaac
     5401 tatcctaagg gtttttaatc atatgtcaat aatttctata atacattatt aaaacatcaa
     5461 ttaaataagt tttaaaattt tacacatatt tttatttaaa aaagatgtat aattaatgta
     5521 ttaaatatag aaagaagttg atattatgaa aaagtgtatt aagactttgt ttttaagtat
     5581 cattttagta gtgatgagtg gttggtatca ttcagcacat gcgtcagatt cgttgagtaa
     5641 aagtccagaa aattggatga gtaaacttga tgatggaaaa catttaactg agattaatat
     5701 accgggttca catgatagtg gctcattcac tttaaaggat ccagtaaaat cagtttgggc
     5761 aaagactcaa gataaagatt accttaccca aatgaagtcg ggagtcaggt tttttgatat
     5821 tagaggtaga gcaagtgctg ataatatgat ttcagttcat cacggcatgg tttatttgca
     5881 tcatgaatta ggaaaatttc tcgatgatgc taaatattac ttgagtgctt atccaaacga
     5941 aacaattgtg atgtctatga aaaaggacta cgatagcgat tctaaagtta cgaagacatt
     6001 tgaagaaatt tttagagaat attattataa taacccgcaa tatcagaatc ttttttacac
     6061 aggaagtaat gcgaatccta ctttaaaaga aacgaaaggt aaaattgtcc tattcaatag
     6121 aatggggggt acgtacataa aaagtggtta tggtgctgac acgtcaggta ttcaatgggc
     6181 agacaatgcg acatttgaaa cgaaaattaa taatggtagc ttaaatttaa aagtacaaga
     6241 tgagtataaa gattactatg ataaaaaagt tgaagctgtt aaaaatttat tggctaaagc
     6301 taaaacggat agtaacaaag acaatgtata tgtgaatttc ttgagtgtag cgtctggagg
     6361 cagcgcattt aatagtactt ataactatgc atcacatata aatcctgaaa ttgcaaaaac
     6421 gattaaagca aatgggaaag ctagaacggg ttggctgatt gttgactatg caggatatac
     6481 gtggcctgga tatgatgata tcgtaagtga aattatagat agtaataaat aaggattcaa
     6541 taatgatatt aagacgagta tgaaaatagt tagattctaa ttattttcac tactcgtttt
     6601 tattttgaaa ataagtaata attcaacaat attataaatt gaacagattg tttgtgaaat
     6661 ttttgataat attaaagtga aaaagtgtta taaattgata aatatatgta attaacaaaa
     6721 acaaatcatt ttaaaaagaa gagagttgta agatgatgaa acgattaaac aaattagtgt
     6781 taggcattat ttttctgttt ttagtcatta gtatcactgc tggttgtggc ataggtaaag
     6841 aagcggaagt taagaaaagc tttgaaaaaa cattgagtat gtaccctatt aaaaatctag
     6901 aggatttata cgataaggaa ggctatcgtg atgatcagtt tgataaaaat gataaaggta
     6961 catggattat aaattctgaa atggttattc aacctaataa tgaagatatg gtagctaaag
     7021 gcatggttct atatatgaat agaaatacca aaacaacaaa tggttactac tatgtcgatg
     7081 tgactaagga cgaggatgaa ggaaaaccgc acgacaatga aaaaagatat cc
//
LOCUS       DALAAR010000108         6981 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000108 DALAAR010000000
VERSION     DALAAR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.111"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(222..917)
                     /locus_tag="O2458_002517"
     CDS             complement(222..917)
                     /locus_tag="O2458_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888360.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(917..1858)
                     /locus_tag="O2458_002518"
     CDS             complement(917..1858)
                     /locus_tag="O2458_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3888361.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(1875..2510)
                     /locus_tag="O2458_002519"
     CDS             complement(1875..2510)
                     /locus_tag="O2458_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888362.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(2507..3733)
                     /locus_tag="O2458_002520"
     CDS             complement(2507..3733)
                     /locus_tag="O2458_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888363.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(4060..4179)
                     /locus_tag="O2458_002521"
     CDS             complement(4060..4179)
                     /locus_tag="O2458_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888364.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(4259..4399)
                     /locus_tag="O2458_002522"
     CDS             complement(4259..4399)
                     /locus_tag="O2458_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888365.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(4403..5002)
                     /locus_tag="O2458_002523"
     CDS             complement(4403..5002)
                     /locus_tag="O2458_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCZ3888366.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            5182..6819
                     /locus_tag="O2458_002524"
     CDS             5182..6819
                     /locus_tag="O2458_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ3888367.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
BASE COUNT         2170 a         1363 c         1042 g         2406 t
ORIGIN      
        1 tttgcagaaa attataaata aaacatctca ttttttagta tttttacaat aattaacgct
       61 ttttatccaa aaactttcat gataataatt gctattttag tgtaaacgca tacaaacaca
      121 acctataaaa aggctactca aagattattt gagctccttg agtagcctca ttttttataa
      181 atcatgaaac gaaatcgcta aattataaac atcttctact attacatatt aatactttgc
      241 ggccgatgat tagattgatt tttacgatgt cgtgttgttg ggtctaatcg tttttctaaa
      301 aatcttaata gtacatcaat gacgattgca atgatagcaa tcggaatcgc acctgctaaa
      361 ataaacgttg tgccatccgt cgcatttgta ccacgaatca caatgtcacc aagcgtaggt
      421 gctcctataa atgatccaac ggcaacaaca cctatcgcaa caaccaaggc aatacgaatg
      481 ccaccgataa taaccgaaac agataacggt aattcaatca ttcgtagcac ttgattgcgt
      541 gtcattccca tacctttgcc agcatcctta atattcgcat caacactagc tataccagta
      601 taagtgtttt ttataattgg aagtaacgca tataaaaaca ctgttaaaac tactgtttct
      661 gaacctaagc ccatgactaa cattaaaata gctaacattg caatgactgg aactgtttga
      721 attatatttg caattgtaat tacaaatcca gaaagttttg tgtatcttgc aagcaagatt
      781 cccaatggaa taccaattaa agctgcaaac agcacaccat agacagacat taataagtgt
      841 ttgaaaaata aatcccatag ataaccaaag ttcgtaacat aataattgaa taattgctgt
      901 aataaattac cttccattac ttatgaccac ctttctgttt atcaaaatag tggtgttttt
      961 tcaaaaattc ttctgcgacg acagcaggtt ctttaccttt accatccgct tcataattca
     1021 agcgttgcat ctctgaagtc gaaatctttc ctgtcaactt attaatcgtc gttttaagtt
     1081 ctgggtgttg ccgtaataat tcatttgttg caacagcact cgcagcataa ggtgggaaaa
     1141 attgtttatc atctttaagt actttcaaat catacgccgc aattcgacca tctgtagaat
     1201 aacctaatgc aacgtctaac ttctctgagt ttaatgcgtc gtagactaga cctatttgca
     1261 ttggtctcac tgtaccaaag tcaaaaccat actctttttt aaatccttca tagccatcgc
     1321 ctttacgatt catccatgaa ctatccatac ctaaacgtaa atctttacta tgctttgcta
     1381 aatctgaaac tgtctctaaa tgatattttt tagcggtttc cttcgttacc atgaatgcat
     1441 acgtattcgc aaaaccatac gaatcaaaaa acgtttgatc aaatttcttt ttaaatcctt
     1501 gttgtgttgc tatcattgct ttcttaggat ttttaattgg tgcttccttc aaagctcccg
     1561 ttaaatctgt gccattatat ctaacacctg atatattagc atccccatta attaaggcat
     1621 tatgttgaat cgtacttgac cctaaattat ttactaatgt tggctttatc ttaccgtgtg
     1681 tatcatgctc tattaacaac cgtaacatat gtgaaataat ttgcgattcg cttgttgata
     1741 atgctgtaat tttgacatca tttttcgtgc tcttactacc tagtccgggc aaactacatc
     1801 cagataatac ggtaagcgat aagacaaaca cgacaagtat atatttaatt ttcttcatta
     1861 tcttagcctc ctaattatcc agatactttt aagcctttgg gaactaccca tttttcaact
     1921 aaagctaata aggcatcaac acctaatgct agtgcagtaa cgagtaccgt tgcagttaca
     1981 atcatcagtg gatcatataa atttaaacca ttgaaaatga aatcacctaa tccacccgca
     2041 cctacataac ttgcaagtgt agcccaacta attacataca cagatgacaa acgaatgcca
     2101 ccaatgataa gcggcaatgc taacggcaat tcaacatcct tcatcaattg aaattgtgtc
     2161 attcccatac tttttccagc ttctttaatg ttgctatcaa tattttgaac accgagtacc
     2221 gtgttattta aaataggtaa taatacataa ataaatagcg ctacaattgc aggcgtttta
     2281 ccaacaccaa aaatcggtat cataatagca agtacagcta gtgttggaat agtttgtaag
     2341 acacctgcca cagttaatac aatattggca gttcgctttg tttttgataa taaaatgcct
     2401 ataggtactg caacaatgat ggcaagtaat aatgccacta tagaaatata gaaatgttcc
     2461 agtgttttcg acatcaactg tccaccatgt tcatgtagga actcaatcat gatttatcat
     2521 ctcctatgtc acgtactttg acattagtag tgtgttgctc atgcactttt gaggacgcag
     2581 tgtcttcccc cacatgttct gtttgcactg tatcctcact atcgccccaa atcgtgtcat
     2641 atacaatatc aacaacattg gcacgcgtaa tcagtcctac taaacgctgt tgatcatcta
     2701 cgacaggtac attcctaacg tttcttttta aaatcgtacg tacagaatct tgtaatttag
     2761 aatcaatttg aacggtataa atatgttgtt gcatggtgtc tcgtaaactt ttgtgtccac
     2821 gtataccctg atttatatct tcaatgtcta agaaacctag taaatggtta ttactatcta
     2881 ctacaaaaat agcatcaaca cgtttttgtc tcataatatg aacggcgtca ttcagtgttg
     2941 cttctgcttg tatcgtgatt ggtttaatca ttacaccttc tacagtcttg tcattgggac
     3001 ggtcttgaat cagtctattt tgtcctataa aatcacgtac aaaatcattt gcgggatgtc
     3061 ttaaaatatt gtctggcgta tcaaattgca ccaccttacc ttctgacata atacaaattt
     3121 tgtctgctaa tttaatcgct tcatccatat catgtgttac aaagataaac gtcttgccta
     3181 attttcgttg taacgtttta actaaatctt gtaacgtatc tctcgtaata ggatccaatg
     3241 caccaaaagg ttcatccatt aaaataatat cttgttcggc cgcaagtgct cttacaacac
     3301 cgatacgttg ttgttgccca cctgatattt ctgctggata acgctctaaa aatgactccg
     3361 gtaaatccac aagtttaatt aattcctttg cacgtttatc cttttcctct ttagtccatt
     3421 tcaacaattt gggtaccaac acaatattct ctttaatcgt catatgaggc attaagccaa
     3481 tttgttgaat aacatagcca atatttctac gcaattcgac aggattcata ctccgaacat
     3541 ctttaccatc aatttcaatt tgtccttctg tcgcttcaat catacggttt atcattctta
     3601 aagcagtcgt tttgccactt ccactggttc caataaatgc gataaattcc ccagattgaa
     3661 tatctaaaga gatgtcatct actgcctttt tattaccaga ataaattttc gttaaatgct
     3721 taatacttaa cataatcatt tctccttcct cataaaaaat atgacgaatg atacgcataa
     3781 aataaaaaga ccattatcgc aattgttaaa caaatagagt cataagttaa acaagtacac
     3841 atcatggtct tgattatgta gaattttata tttttcatct gtaatgtgaa tacttaacag
     3901 taaacggcgc tatgtatgtt gaatttctac cgattacttt gtttgcacat tcgtatattc
     3961 gacatttaaa actaacatac tctatatgat gttattaaac attattattt tatctttggc
     4021 aactaaatag ataactcata ttttttaaaa gcgaatttat tatcgctcaa ttggttgttt
     4081 tatagcaaaa aagtgtcgaa aatgtgaaat attattacaa aagtcgaatt tttgtaataa
     4141 tgcacacgca tccattgtta cagacggaca accgcccatc cgccactcac aaagtcatcc
     4201 ttatatttta atatcttcat cattcattgt agacaccctt gccttttgct aacattttct
     4261 actcccaagc ctgtaacgga attccaacgt caaagtgttg cccatgacag acacacctaa
     4321 aaaaacaacc aacagcatga gccattggtt gtttcgtgtc atcttgtatg ttgttgtata
     4381 accggtatgc tttactcatt aattacttat catgcatccc tttaccttct agtatttggt
     4441 taatatactt ttcaacacgc ttttgtcttg tttcactgcg tttagcttgt ccaatatgat
     4501 atatatattg atgttgtctg cctggcgtta atttataaaa tgaagactct aactgtggca
     4561 tttcttcgaa cttagcttct aactctttag gaataacata ttcctctgtt ttcttcattt
     4621 caactttttt accagctttt tcagctttaa ttgcttcggc tagataatat ttaatttctt
     4681 cggtacgtgc ttgaatctct gttaaatttt caaatcgtaa ctgacgagct gcttgcacct
     4741 tttcagtctg ttgaatgagt gtatgatatt tatcctccaa aatggcacct ttatgaaata
     4801 atagtgcaac ataatttttg aagccatgga taagtactac atttttatta ttcaacgtgt
     4861 aacaaggatg catccattta tacgcttcct ctaattctgt ttcattaaag attaacgctc
     4921 tcaaatattt atattcttct tgccattgac tctctttatt taaaaatgct tcaacttgtt
     4981 catttgattg tttctcactc atagtcatca cttccatttt gtttttcaat acacattgta
     5041 ttatatatcc taattttata aaaaactaat tttacaaatg cttttgcgtt cttacaaaaa
     5101 atgcatttga ctattattat aataagcgta taattgtcgc atattatttt ttgtattttt
     5161 ggcaataacg aaggagtatt tatgaataaa gacaagcaat tgcacaacga caaaatcaat
     5221 ctatcccaat tagtcttatt agggttaggc tctttaatag gatctggttg gctatttggt
     5281 gcgtgggaag catcatcaat agctggacca gcagcaatca tatcatgggt tcttggattc
     5341 ctagtcattg gaaccattgc ctataactac attgaaatcg gcacaatgtt tcctcaatca
     5401 ggtggcatga gtaactatgc ccagtataca catggctcat tattaggctt tattgctgct
     5461 tgggcgaatt gggtgtcttt ggtgacaata atacctatcg aagctgtgtc agctgttcaa
     5521 tatatgagtt cttggccgtg gcattgggcg aaaccaatga gatatttaat ggaaaatggc
     5581 tctattagca catacggatt gctagctgta tatctcatca ttgttatttt ttcattatta
     5641 aactattggt ccgtaaaact tttaacatca tttacgagtt taatttctgt atttaaatta
     5701 ggcgtaccca tgttaaccat catcatgttg atgctatcag gattcgacac ttcaaattac
     5761 ggccattcgg caagcacatt tatgccttac ggaagtgcac cgatttttgc tgcaacaaca
     5821 gcatcaggga ttattttttc attcaattca ttccagacaa ttattaatat gggttcagaa
     5881 attaaaaatc ctgaaaaaaa tatcgcaaga ggcatcgcta tctcactgtc aatcagtgca
     5941 gtgttgtaca tcattttaca aagtacgttt atcacttcta tgcctcaatc aatgttacaa
     6001 catagtggat ggaatggcat caacttcaat tcaccatttg ctgatttagc tatcttatta
     6061 ggaattaatt ggctcgcaat tttactatac attgaagctt ttgtatcacc attcggtact
     6121 ggcgtgtcat ttgtcgccgt tacaggtcga gttttacgag caatggagaa aaatggacat
     6181 atccctaaat ttcttgggaa gatgaatgaa aaatatcata tcccacgtgt agcaatcatc
     6241 tttaatgcca tcattagtat gattatggtt acattattta gagattgggg tacgctagca
     6301 gcagttattt ctactgcaac tttagtagcc tatttaactg gcccaacgac agtgattgca
     6361 ttaagaaaaa tgggaccaac aatgactcgt ccatttagag caaaaatttt aaaagtaatg
     6421 gcaccattat catttgtatt agcttcatta gctatatatt gggcaatgtg gccaacaacg
     6481 gctgaagtta ttttaatcat tatacttgga ttaccaatct acttcttcta tgaatatcgt
     6541 atgaattggc gtaatacaaa gaaacaaatt ggtggtagct tatggattat tgtatattta
     6601 atcgtgctat caatactgtc atttatagga agcaaagaat ttaaaggctt aaatatgatt
     6661 cactatccat ttgactttat cgttattatt attgtggcac ttatcttcta ttacatcggt
     6721 acaacgagtt catttgaaag cgtctatttc cgtcgcgcaa cacgaatcaa tacgaagatg
     6781 cgtgagtcac taaataatga atcaaagtca tctcattaaa tattttaaaa tacagtgcca
     6841 tatatgattt caacacacac acattaacca accattgatt tcaacatctt ggttggtttt
     6901 ttattttgaa aatcggttat aaataactaa cataacaaga tgatgatcag gctgggacat
     6961 aaatcaatgt tctatgctct a
//
LOCUS       DALAAR010000109         6872 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000109 DALAAR010000000
VERSION     DALAAR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.112"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(271..1899)
                     /locus_tag="O2458_002525"
     CDS             complement(271..1899)
                     /locus_tag="O2458_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888368.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(1921..3270)
                     /locus_tag="O2458_002526"
     CDS             complement(1921..3270)
                     /locus_tag="O2458_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888369.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(3504..5291)
                     /locus_tag="O2458_002527"
     CDS             complement(3504..5291)
                     /locus_tag="O2458_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCZ3888370.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(5291..5581)
                     /locus_tag="O2458_002528"
     CDS             complement(5291..5581)
                     /locus_tag="O2458_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888371.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            5720..6769
                     /locus_tag="O2458_002529"
     CDS             5720..6769
                     /locus_tag="O2458_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCZ3888372.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
BASE COUNT         2136 a         1060 c         1178 g         2498 t
ORIGIN      
        1 aaaaagtaac gggattttgg gattgtgctt gcacaatcct tctgtttctt cgaatctgca
       61 aatcccaatc atttcccgat aaaaaatcat tgtgggatgt tctttagcaa tttcaatata
      121 agcattgtat agttatgaaa aaattacgac aataactgtt tcattagata agtgttattg
      181 aaattgataa agagcaattc ttgaaaatag ttagataaaa taagcgaaag aatatagtga
      241 aaattattgt tataacaatg attctattag ctaaatagta agatatagtg tttggggcaa
      301 aaataaagac gaagtgctga gatgcacttc gtcgagttgt ttattattga aaagttgttt
      361 aatgatttcg ttattaagtt tgagtgtgac atagaattgt tttttatgat taccatcttt
      421 tttaatatca atgcgatcaa tcactgatag atacaatgct ttgagtcgag atttttctat
      481 gtgcttaata tcatgaaaga tgtgttgtaa tagtttactg atttctttgg catcaaataa
      541 agtcttatct tcattttgtt gatttttgag ttggttgatt tgattcgtaa tgtcattgag
      601 ttgcttttca tatttttgaa tacttggtct gattactgat gttaagtccg gattatcctc
      661 gatggtttta atcaagttat ttattttgat ttgtacttca tcatattgtt gttgcttata
      721 agcaatatcg tgatgaagtg cagcgccatc aacttgattt tcttgattga cgtgtgttac
      781 tacgcgttga atgactttat cacttttgac tatttcaagt atttgcttca tcacataatc
      841 ttcaatcaca tcagctctta cactgtttgc cgaacatact ttggaaccct tgttccgaaa
      901 attactacat gaatagtaac gaatacgttt cttagtcccg tctttaagag tattcgtggt
      961 attgcttgct gccataggtg cgccacattg gggacagtga ataatgcctg taagcagatt
     1021 cgttcctttg ccatggactt ggggtttttg actgacttgt ttcttacgca tttgtacttt
     1081 atcccataaa tcttgattaa taatggggga atgcttacct tcagctatca ctggtttatc
     1141 attcagccct ttacgacgtt tttcactcca atctttgtat ttcgcaaatt gaattttgcc
     1201 gatatagaaa gggttagcta agatgtatgt gattgaacta atactgaaag gtttcccctt
     1261 tttagtgaca tatcctttgt gattcaatgc attggcaatt ttacgatagc catgtccttt
     1321 ggcatagcac tcgaatatat attttacaat attagcttca tgttggttaa tcattagctc
     1381 gtgtttacta tctggtattt tgtcataacc tagtggtaaa ttgccttgat aatagccttc
     1441 ttgggcacgt ctcgtttgac ccataaatac gttctcgaca atgttattac gttcgaattc
     1501 tgagaaactc gcaagtatct gtaacatgag tttaccagaa gaagtattga cttccatacg
     1561 ctctgacaaa ctgaaaaatt cgacattttg tttgtgtaaa tcttcgacaa ttttgagaag
     1621 atcagatgta ttacgagcta atcggtttgt tttgtatacc ataacacagt cgatattgcc
     1681 ttcttttgca tctttcaaca tacgttggag ctcaggtctg ttcatagatt tacctgaaat
     1741 accacggtca gcgtatatat ctttaacttc aaaatgatgg aagtcacagt attctttgat
     1801 ttgattgatt tgtccgtcga tactataacc ttctgtgctt tgcatttctg ttgatacacg
     1861 tacatagata ccgacacgtt ttgttttaag ttgttgcatt atgttacatc ctttcttcat
     1921 ttatgcaatc gatgattgca tggtttgatt gacaatattg agtggttcat ttttgaaata
     1981 gattcctata agatttttat ctttcgtaat gtgaatggtt tcaatatagg ggtacaatat
     2041 gtttaacgtg aaacgttttt gaataatatt ttgaatgatg tgttgtattt gatgcccatt
     2101 gatagatgta gtgcgttgcg gttgttgacg taatgattgt gtttgctctc tgaacgtttc
     2161 tgcatcaatg atgccttgtg ccaacttttc tatcagttgt tcttgagtca atgtgtgatg
     2221 tttttctatg tttctttgtc ttttgatgcg tttgtcaatc gcacttttaa tttttgtgta
     2281 gatgcgttga ttttgataaa agtctcggca cacttctaat actttatctt caagtgtttg
     2341 tgcattgatg cctttaaaat cacagacaaa gcgtgaagca ttcatgtttt taggacagac
     2401 gtagtaacgt aatgtatgat tcttttttct aatggtcata tttgtaagtg ttgcattaca
     2461 acatgggcat ttgatttttt gtttgagttg attatccgaa ggtgtctgtt tggtttgttt
     2521 ttgcaatcga agtctctgcg cttgctcata tatacttgtg gaaacaatag aaggaaacat
     2581 attgtcgaat tggccatatt gattgttgac acgaccacaa taattaggat tgatgataat
     2641 gttacgaact tgatagggtt gtcgattgat atacgtgtta tcttcttcta ataactgtgc
     2701 aattttctta taaccatgac ctttaatgta ataattgaat acagccttta ccgttggtga
     2761 ctcattttga ttgatgatga atgttccgtt gtgatattcg taaccaaaag gtgcatgtgt
     2821 tgtaatcaat cgaccttgct ttgctttttc ttgaagccca tttctgactt gttctccaat
     2881 gttatccgat tcaagttcgg ctaaactgat gaagatatta agcttgagtc ggtcgaaagc
     2941 ttgatccata tcaaagtaac catcatgtac gcttaagata tgaacatggt acgtttgaca
     3001 taatttgatg agttttaatg catttttcag attacgatgc aacctattaa gacgataaca
     3061 gcataatatg tcacactgtc cttgttgaat taattgtgtt atttgtcgat acccactacg
     3121 attatctttg cgacctgatt gtttgtcgct ataaaagttg atatgttgaa tatgatgttt
     3181 ttcggctatt gcttcgatag cttgtttctg tgctgcaaga gattgttgtt tcatcgtact
     3241 ttgacgtaaa tagcctatga cttgtttcat atcggctcct cctttcacag tgataatata
     3301 tatttatgga tgaattgata tataagccca acatcaatga gatgttgggc gtccatatta
     3361 gtcatttgtt tgattgattt cttcaattac caaatcggct aatatctcga taagttcatc
     3421 catgtttttc actccgttat ttgttctatc ttcaatacgt cgattattca gtttgatgct
     3481 tcacagttgt atgataaaga caattagaaa tcttcgtgaa ctcctgaagg gcctatccct
     3541 tcattagcgg atttaaaaag ttctttcgca gctttgttat catttgacgg tgtccaattt
     3601 tgaagtaacg acttatcttt agttaatccg aggatagatg caaactctac atctaatttt
     3661 agatggtaaa atacaagtga ttgtttttta ccgctattat ctttgacact tcttttagtt
     3721 gtttggcgtc cacggtcagc taatatgaaa cctttatctc ttaaggcgtt gacaacatta
     3781 ttaacatctt gaaattgatg attgtttagc atctgtttaa aaacgttcgc aattattttt
     3841 acttcgatat ggtcatcttt taatgagatt aatccatagt tctcaaacat atttttcaaa
     3901 gcaccttcat ctgaaaactt acctctgttt tgtgccacaa attgaatgat gacatcaata
     3961 gctttatcag ctaatgagcg ttcagagact gtatgagtat gataatcaat aaagtagtct
     4021 cttatattag cgatatcaat atctgtagat aaaacacgac ctaatatttt cgcagatgtt
     4081 gtaatgactg cataacgctt aaacatacga atacctgtat tgtttgtttc atctttcaat
     4141 ttagcttcaa accaatctac ttccttgtaa aaccattgaa taacttcatc ttcacgattt
     4201 ataagatatt tagctactaa cggtaaaaca tgaccatagt ttagtgctac agctttttta
     4261 atattgtcag cattggtcgc atttgtagtg aattgttcat taatctcgat ggttcttaca
     4321 cgtaatccat cgttttgagc tgaatcatta aaaatactgt attctgacgt tgaaatgaca
     4381 gaagtacccc aattcttagg cgttttaact tctccatgaa cgtttgaacg ttgacgacct
     4441 tgaccttcag cgatggaata taacaaaccc gtggtatctc taagtgtcgt agatgaaagc
     4501 tcatcaaata ctataggaat gccataattg ttactcaaat aaccttcaag tgcgtttcgt
     4561 gtggcattcc aacttctaaa aagagtttca ttacctttgg tagggttacc agcgactgat
     4621 acagctaaag cagctgcggt tgacttaccg gttgaggatt gacctgtaaa actaaagaga
     4681 attcctgcaa attcgatttc atgtttatgc ttcagaaaac ttgtcactaa ggcagaaatc
     4741 ccaaatatga ctgccaattc taaaagaaga tgacctttaa cctcgtcaat atacatgtta
     4801 aaccaattat caaatgtacc tttaggagtt aagtcataag cactatctac aatagggtat
     4861 gaaggtgagg actgattaaa ttgaatagat ttgagaactt tatctagcga tatcaagtag
     4921 ccaaaaggtg tctctagtat acctacaccc tcatataatt cagaaagtgg taatctatct
     4981 cgcatcaatt gcaaggcata actcaatgat ctaatatact tctcgttaat actgtagcca
     5041 tatttaatta aagaaggtaa gtttcgtgtt gttaagatat cagactcaaa aatgtctttt
     5101 ttaccattgt tgttagtaat aatcaacttt tcaacaccag taattggatc agaaaatctt
     5161 gcattaacaa taatagggct agccattcta accacttttt cactgtcacc atctttttta
     5221 ggtggtatcg tttctttcca accgaaggaa tctaaagcaa atgggccaat ttcaaacaat
     5281 gtgtaactca ttagcaatca cctcctttcg aaggcgtgct attattgcat gggtttatcc
     5341 ctattttctg atataataaa ctgccatttt ttgttcctac aattttaaat ggtaatcctg
     5401 gcgtgtactt aatccagtaa gcaaaacgac gtcctacttc acgttgatca ttcgtgctac
     5461 aaaccacatt atatttcttt cttaattcac cataagtgaa cgtacttcca actggttgtt
     5521 gaaaagattt agcaattata aaattataaa gcttttcttg ctgcttagat ttcttattca
     5581 ttttaaaatt cctccaataa tttttaagga gtcaatcaat atcatatagg ttaaactatc
     5641 ttcaattgat tgctataata agcataaatg aagcattatg attagacgcg gaacaaaaaa
     5701 tggggagggg gggtatatat tgatacctac gctagaagaa attattgata agtatggaaa
     5761 cttagttgat tatttgaaat ttgatgtaac agttgaggtt tatgaagatt tgttgttatt
     5821 aagaagttta agcgatttaa atgaacatca aaagatggat agagtttcat ttataaaaaa
     5881 acatttaaac tcaaatttcg attatttatt agatgatact gagtttagaa gtatcaataa
     5941 gatttacagt aatttaaata tcatgacgca tatcaatagt caaaataata attttaaacg
     6001 taatccattt ataaatgaag aacaattgaa atcactactc gcgataaaga aagtagatgc
     6061 acatatgagt tatgattcaa atattacttc aaaagcattg gcagaaataa acaaaacgca
     6121 aaaaagatta gttacaaaga tagacacact ctataatcag tcaaaaaaag aaaatgaata
     6181 tgtacggtta ggagaaaaaa tatcgtatac aaaattggag aatcattgga aatatttata
     6241 taatgagatt cagttttata atcttaataa tcaattgatt agttatgttg cattagaaca
     6301 ggaatatgct tgggcttttt taaatgaact attttatttg atagaattgt atttgaaggc
     6361 atttaaagga caaaagaaaa cagattatga gtttgggaaa aaattaaacg aatttattca
     6421 atcatatgtc attattttac ttatagatat acgattacct gttgttagat tatatattat
     6481 tagagacatt gtagatacat gtgaaggaga aaaagataat tacaaaagaa ttacactgat
     6541 agaagaatct attaaaaagt atagatacgt aaaagatcag attaaaagat ttaaaaatgg
     6601 attagaagaa tcgttattaa atgcaacttt atctattgaa caaaatatgt tgaaagttaa
     6661 aattgattat tataaagcct actgctttcc tagagaaaaa acaaagcata acaatattga
     6721 gtacaatgta tcgttgtttt ttaaagcatt agatgcctta aagaaatagt ttttcactga
     6781 aaatatttat agatataatt aaaattaatt aatattaagc caggataatg tagtcttaat
     6841 cgttctgaaa tacgaaaaat gttgtggaaa tc
//
LOCUS       DALAAR010000110         6855 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000110 DALAAR010000000
VERSION     DALAAR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.114"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            173..1162
                     /gene="trpS"
                     /locus_tag="O2458_002530"
     CDS             173..1162
                     /gene="trpS"
                     /locus_tag="O2458_002530"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCZ3888373.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1204..2085)
                     /locus_tag="O2458_002531"
     CDS             complement(1204..2085)
                     /locus_tag="O2458_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888374.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(2097..3059)
                     /locus_tag="O2458_002532"
     CDS             complement(2097..3059)
                     /locus_tag="O2458_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888375.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(3052..4032)
                     /locus_tag="O2458_002533"
     CDS             complement(3052..4032)
                     /locus_tag="O2458_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888376.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            complement(4035..5021)
                     /locus_tag="O2458_002534"
     CDS             complement(4035..5021)
                     /locus_tag="O2458_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888377.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            complement(5072..6787)
                     /locus_tag="O2458_002535"
     CDS             complement(5072..6787)
                     /locus_tag="O2458_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ3888378.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
BASE COUNT         2241 a         1206 c          935 g         2473 t
ORIGIN      
        1 aaattaaatg agaaaaacct aatttttcag ataagtttct acacttataa aaaagattat
       61 taatcccttt gttagtagta agttatacgt atattctaac acatcttaca tttttaagaa
      121 atactgttat aatgataatt attaaaatat tactaagaaa gtaggcattt aaatggagac
      181 attattttca ggcatccaac ctagtggaat tcctactatt ggaaattata ttggcgcact
      241 aaaacaattt gttgatgtgc aaaatgacta tgattgttat ttctgtatcg tagatcaaca
      301 tgcaattaca atgccacaag atcgtttaaa attacgtaaa cagaccagac aattagcagc
      361 gatttattta gcttctggta tagatccaga caaagcaaca ttgttcatac aatctgaagt
      421 ccctgcacac gtacaagcag gatggatgtt aactacgatt gcttctgttg gagaattaga
      481 gcgtatgacg caatacaaag ataaagctca gaaagcagtt gaaggtatac ctgctggtct
      541 attaacatat ccacctttaa tggcagctga tattgttctt tacaatacta atatcgttcc
      601 agttggagat gaccaaaagc agcatatcga attgactcgt aaccttgtag atagatttaa
      661 tagtcgctat aatgatgtgc ttgtgaaacc tgaaattcgt atgcctaaag ttggtggacg
      721 tgtcatgagt ttacaagacc caacaagaaa aatgagtaag agtgatgata atgctaaaaa
      781 cttcatttca ttattagacg agccgaatgt tgcagctaaa aaaattaaaa gcgcagtaac
      841 tgattcagat ggtattatta aatttgatcg tgacaacaag ccaggtataa caaatttaat
      901 ttcaatatac gctggattaa cagacatgcc aattaaagat attgaggcaa aatatgaggg
      961 cgaaggttat ggtaaattta aaggtgacct tgctgaaata gttaaagcat ttttagtaga
     1021 attccaagaa aaatacgaaa gtttctataa ctcagataaa cttgatgata ttttagatca
     1081 aggtagagat aaagcacaca aagtttcatt taaaactgtc aaaaaaatgg aaaaagcgat
     1141 gggtttagga cgtaagagat aaaataaaga ggtactagaa ttaactagtg cctcttttat
     1201 tatttaacgt ctacctctag gattaaaagc atctttaatc ccttccccta caaagtttat
     1261 acttaatata gttaatgtaa tcattatagc aggtggcatc catatccacg gtttaccact
     1321 tataacatcc ccttcttgag catcactcaa catattaccc caagatggta ttgctttact
     1381 aattccgaat cctaaaaagc tcaatcctga ttccactaca atcataccgg caaataacaa
     1441 tgttgcttgt acgataacta cacttaatat attcggcaaa agatgtttca aaataatttt
     1501 atatgtgggt gtaccaattg attttgctgc caaaaagtat tcattttctt tttcttgaag
     1561 tactttacca cgaacaagtc ttgcaatacc tccccaactt aatacgataa taactagaac
     1621 aagaattatg gcagatccat aaggattttt aattttatct ccaagtgcag catttaatac
     1681 aattgcaaat attaaaaatg gaaataacat aacaaattcg gttattctca ttaataatgt
     1741 atcaacaata cctccaaaat aaccagaaat cactccaact gtaattccaa tcaaaagcat
     1801 tcctattgta gatgtaattc caatggataa tgaaatacga cctgcatata acaaacgact
     1861 aaagttatcc ctaccaccag agtctgtacc aagaatgttt tgtgctgtca tttcaccttt
     1921 tatatttaat aaatcttgtt ggttcactgg aaaaggagct attaatggcg ctataattga
     1981 tacaatagtt atgattaata aaaaaataac cgataacatt gcaattttat tatgaataaa
     2041 tttagaaaat gcaattttaa aaggcgattt tgacttattt tgcattctaa tcccccctag
     2101 ttacttctaa ttcttggatc tataaatcca taagtaatat cagaaatcaa attacccagt
     2161 aaacctaaaa atgagaaaaa taacgttaat gccatcatta atggatagtc ttggcctatt
     2221 accgattcta aaaataattt accgataccg ttatatgaaa agatagtctc cgtaatcacg
     2281 gctccaccta aaatacttac aatatcagca cctaaaaatg taataatagg tattagagaa
     2341 tttctcaata tatgtttatt ataaatttta ttcatagata atccttttga ccttgccgtc
     2401 aatacataat cttttttaga attttcaata atatcattac gtaaatattg aatataacca
     2461 gcagtagata ataaacctaa agtgaatgca ggtaaaaatg tgtgatatat tttactcata
     2521 taatattcaa aagtaccttc tttaaggtta atatcaacag aaccttgaaa cgggaaccat
     2581 tgtaattcaa aagaaaaaat aaaaattgca aatactcccg caataaaaga tggaatggct
     2641 aacatcaaat aattaaatat ttgcacagta taatccgtca aactgtaaga atatctacct
     2701 gacgttattc ctaaagcaaa tgagataata taagtaataa ttaatgacat agcaccgagt
     2761 aatattgtat ttggaattct ttcctcaata acatcaatta ccggcctttt atatttgatt
     2821 gactcaccta aatcaccgtg tataacatga ttgacccatc taatgtattg atgtggtatt
     2881 gagtcgttga gacctaattt ttccctttgt gcatttattg cttcttgttt tattttaccg
     2941 ttttgtagat ctgaaaatgg atctcctggt tgtatgattg ctaatgaaaa tacaactatt
     3001 gagattaaaa ttagtaacgg gatcattaac cctaatcttt taagtattaa tttaatcatt
     3061 taaatcacct actttatcta cgtagaaaca agcaacttga tgttcattac caatgtcctt
     3121 taattctggc atactttttt tgcacatatc ttgagcaatt ggacatcttg tatgaaaagg
     3181 acaaccttgc ggcggattac ttggcgaagg taattctcct tttaatataa ttctattgtt
     3241 attatgttta tcaatttgtg gtattgatga aatcaacgct tttgtatatg gatgtttggg
     3301 attttcataa atttctttat cagatgcgat ttcaactata tgacctaaat acataactcc
     3361 aatgacatca cttatatgtt ttactacact taaatcatgt gcgataaata aatagcttaa
     3421 gttaaattgt tcttgtaaat cttttaataa attcagtact tgagattgaa cagatacatc
     3481 taatgcactt acaggctcat cagcaacaat taaactcgga cgcaaagcca atgctcttgc
     3541 aattcccact ctttgtctct gtccacctga aaattcatgt gcatatttat aatatgcatc
     3601 ttcacttagg ccaacacatt ttaataaata tagtacttct ttttttattt cttcttttgg
     3661 caatttttta taatttaaaa taggttctga aatgatatct ccaaccattt gcatcggatt
     3721 caatgatgca tacggatctt gaaatatcat ctgatattgt tgtcgtgatt ttctgagttt
     3781 tttaccttgt aatcttgtta tatcttcacc attaacaatt attgagcctg aagttgcatc
     3841 ttcaagcctg ataatcactt tacctaacgt tgacttacca caacccgatt ctcctactaa
     3901 tccaaaagtt tgtccttttt ttattgaaaa cgatacgtca tcaacagctt taacgtactg
     3961 agttttttgt aataatccag ttttaatttt gtagtatttt tttatttggt tgacttctaa
     4021 aatattttcc attcttatat ctccttatat tcatttttgt acaaatgaca acgtacaatg
     4081 acatcttcac aaacatgcat tttaggagat tgattattac aaatatccag ttttttgtta
     4141 catctatttg caaatctgca cttattaacg tgaaatgctt ggattgatgg cacaattcct
     4201 tctatcgttt caagtcgttt ctgctcttct ttaagttttg gaattgttga taataatttt
     4261 tgggtataag gatgtttcgg attgtgtagt atttctttta tgcctccaaa ttctacaatt
     4321 tgacctgcat acataactaa gactttatcg caaaactcag caactacact caaatcatgt
     4381 gtaatcatca taattgccat ttgcgtttcc ttttgcaatt cttttaataa gtctaatatt
     4441 tgcgcttgaa tcgtgacatc caatgctgtt gtaggttcat cagcaattaa taatttagga
     4501 gaacatgaaa tggccattgc aatcattact ctttgtctca taccaccaga taattgatga
     4561 ggatagctat ttaatatttc atcttgtcta gcaattccaa ctttttttag taaatctttt
     4621 gctaatttat ttgcttcttt tttagaaatt tttttatgtg attttataga ttccacaagt
     4681 tgatttttta tggtaaatac aggatttaaa gcagtcatag gttcttgaaa aatcatagca
     4741 atatcatttc ctcgaatatc tggcaattgc ttctcattga gcgtatcgat tcgtttacca
     4801 tcaaatataa cttctccatt acttagttta gatatctttt ctggtaataa tttaataatt
     4861 gacttactta atatggactt accgcaacca gattccccta ctatacccaa aacttctcct
     4921 ttagataatt caaaagaaat attttcaaca gttgctaacc attcattatt tatttttaat
     4981 gatgtttcta aatctttaac ctctaacaat acattattca taacatcccc ccatataagt
     5041 aaacctcgac aaaatcgtcg aggttacatt actaagcttc tttagttaaa ttatataaac
     5101 tttgatcagt tccaatttca atgtctaagt ttcgtacttt atcgttaaca atagttatcg
     5161 atttaccttg gaacattggt aatccaggta actcatcatt catatatttt tgccatttaa
     5221 cataaatatc tttacgtttc ttttcatcaa tgcctacttt ttcgaagtct aatgcatcat
     5281 ctaaatattg atctgatttt ggtaaaactg ttctcatttc attttgaggt ctatcagtgt
     5341 ggtataaatc tgatggatct ggatctgtac ctcctgccca tgatctgaag tacacttcca
     5401 tatctttaga tgcatttgct aagtcttcat tatatttacc gaattctact aacttcacat
     5461 ttgttttcaa gccaactttt tcccagaaat cttttatcgc agcagttctt ggttcaaaag
     5521 taggattaga acctgaataa tgtttaaagt taatctcaaa tttattacct ttaggatctt
     5581 ctctaaatcc gtcaccatct ctatctttat aacctaactt atctaagatt tttttagctt
     5641 tttcaggatc atatttgtaa tcatttaggt ccttaggatt ggctgctatc caatgcatag
     5701 atggtacaaa actattgatt tcactagcgt aaccattgaa aaacgctttg atccattttt
     5761 ctctatcaat tgcataaagc attgctttac gtaattcttt gtcttcatat tttggtctca
     5821 ctttaccagt tttattagct tttttatcgt aatcatgaga tacgaatcct attaaaccgt
     5881 agtctaagct tggcgcagat aataccttga gaccagcatt agatgactta gcatcttttg
     5941 ccattgcacc ggtagcatca ttcgcaacat caatatcgcc tttttccatt gccttaataa
     6001 tttgcgcttg atcaataact tttaaattga ttttgtctag tgcaggctta ccttgccaat
     6061 aatcatcaaa tttaacgagt tgaacagcct cacctggaac gattttctta actttatacg
     6121 gtccaatacc aataggatat tttcggattt tatctgattt cgctaaatct ttaattggta
     6181 catctgataa atatttttta cttaataaag gtccagtaat aaatcctgtt aagtaatttt
     6241 cttgtttttt atcaaatgta acctgcatag tgtagtcatc tattttcttc aatccgctaa
     6301 tatgatcagt ttttccttca tgataatctt tcgcaccttg gatattttct acacttggat
     6361 aataagcacc ttcgtagtcc ttgttagcta agacttcaat tgaataaatc caatcatcaa
     6421 ttttcaattc attaccatca tgccatttaa tacctttttt aattttaaat tctattttct
     6481 tagccggctc gatgtccttc catgaagcaa tatatgcctt aggacgtatt tttttatcag
     6541 ttgctaataa gctttcgtta aaatatccct caactacaga atctgcatgt gtactattta
     6601 ataacgaaga ataaacacca cttggcggtg ctgttaatgc tacatttaag gtaccacctt
     6661 tatgttttga ggtttccgtt ttagtagcat cttttactcc ttcatcttta ttactgcttt
     6721 ttccgcaagc actcaacact aaaacgacaa taagaagtat tgaaatatat ttaattagct
     6781 tccccataca tatgcctcct actttcaatc taattttttc tttttagttt actcatagaa
     6841 aaaattatta caagt
//
LOCUS       DALAAR010000111         6736 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000111 DALAAR010000000
VERSION     DALAAR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.115"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            13..1206
                     /gene="fmtA"
                     /locus_tag="O2458_002536"
     CDS             13..1206
                     /gene="fmtA"
                     /locus_tag="O2458_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCZ3888379.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1743..2021)
                     /gene="qoxD"
                     /locus_tag="O2458_002537"
     CDS             complement(1743..2021)
                     /gene="qoxD"
                     /locus_tag="O2458_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCZ3888380.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2030..2635)
                     /gene="qoxC"
                     /locus_tag="O2458_002538"
     CDS             complement(2030..2635)
                     /gene="qoxC"
                     /locus_tag="O2458_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCZ3888381.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2625..4613)
                     /gene="qoxB"
                     /locus_tag="O2458_002539"
     CDS             complement(2625..4613)
                     /gene="qoxB"
                     /locus_tag="O2458_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCZ3888382.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4613..5713)
                     /gene="qoxA"
                     /locus_tag="O2458_002540"
     CDS             complement(4613..5713)
                     /gene="qoxA"
                     /locus_tag="O2458_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCZ3888383.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6015..6137
                     /locus_tag="O2458_002541"
     CDS             6015..6137
                     /locus_tag="O2458_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888384.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6285..6602)
                     /locus_tag="O2458_002542"
     CDS             complement(6285..6602)
                     /locus_tag="O2458_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCZ3888385.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2401 a         1059 c         1144 g         2132 t
ORIGIN      
        1 ggcaattatc aaatgaaatt taataaagta aaactagtta tacatgcgtg tgtactatta
       61 tttatcatta tttctatagc gttaattttt catcgattac agacgaagac acattctata
      121 gacccaatac ataaggaaac aaaattatca gacaatgaaa aatatttagt ggatcgtaat
      181 aaggaaaagg ttgcgccgtc taaactaaaa gaggtatata atagcaagga tcctaaatat
      241 aagaaaattg acaagtattt acaaagttca ttatttaacg gttcagtagc tatatatgaa
      301 aatggcaaat tgaaaatgag taaaggttat ggatatcaag attttgaaaa aggtattaaa
      361 aacacaccga atacgatgtt tttaataggt tcagctcaaa aattttcaac agggttactg
      421 ttaaaacagt tagaagaaga acataaaata aatatcaatg atccagtaag taaatacctt
      481 ccatggttta aaacatctaa gcctatccca ttgaaagatt taatgttgca tcaaagtgga
      541 ttatataaat ataaatcctc aaaagattat aaaaatttag atcaagcagt taaagcgatt
      601 caaaaacgtg gtattgatcc taagaaatac aaaaagcata tgtataacga tgggaattat
      661 ttagtacttg cgaaagtaat tgaagaagtt acaggtaaat cttatgctga gaattattat
      721 acaaaaatag gagatccttt aaaacttcag cacacagcat tttatgatga acaacctttt
      781 aaaaaatatc tagcaaaagg ttatgcttat aatagtacag gactttcatt cttaagacct
      841 aatattttgg accaatacta tggtgcaggt aatttatata tgacaccaac agatatgggt
      901 aaattaatta ctcaaataca acaatataaa ttattcagtc ctaaaataac caatccatta
      961 ttacatgagt ttggtacgaa aaagtatcca gatgaatatc gatatggttt ctatgctaag
     1021 ccaacattga atagacttaa cgggggattc tttggacaag tctttactgt ttactataat
     1081 gataagtatg tagttgtact tgcattaaat gtaaaaggaa acaatgaagt tcgaatcaaa
     1141 catatttata atgatatttt aaaacaaaat aaaccttaca atacgaaggg tgttattgtt
     1201 caataattaa tatagaagat ataacatgta tatggcatta aggcatcgac cttatctgac
     1261 cagtatacga gttatatctt cttttttata gtggtaaaaa gtttaaagta taaggttgaa
     1321 gaaggatgag tttaaaaata tgtgttaact gataaaaggg gaaatcattt ggtgagttgg
     1381 catcagacta aaatgaatga agacgaatac gttggtccat gcgtggtgtg aatgttttct
     1441 aaatctaact ggaaatgata atttaataat gtacactttt gattgtttaa acatgtacaa
     1501 tttttaaaaa cctagggata attttattga cgtgaacaac attgagatta tctttagaca
     1561 taaatatgga ttttgaagtt actatattta ttagttgtgg ttgttaatgt attaattgaa
     1621 ttgattataa tgattgtctt ataagatata gaaggggact taaccattta aaaattgaag
     1681 gaaaataaaa accagctaac aaaagtgtta gctggttaaa ctttacatta ttaaaatgtt
     1741 gtttataagt gtgaagagtg accgccttgc ataacccaat atgttccgac aacgaaacaa
     1801 agtgtaatta caagagcaaa gataactttg aatgtttgta aacgtccatc tttaccttca
     1861 gttaaatgca tgaacattaa taattgaagt cctgcttgga cgaatgcaaa gccaaagata
     1921 attgtcaact tcgcgtggaa tgttaatgac gtgtatagtg ttacgtaaac tgctaaaagc
     1981 gttaatacga tagatgcgat aaatcctaca gtatgtttca ttattgtact catccgctat
     2041 acaccatccc tatcatatat acggcagtaa agatgaaaac ccaaacaaca tctaagaagt
     2101 gccagtataa acttactata aataattttg gcgcattata tttgtctaat ccgcgtcgtt
     2161 ggatttggat taataaacaa atggcccaaa cgatacctag cgatacgtga caaccatgcg
     2221 ttcctaatag gataaagaaa ctagaccagt aagaaccaat tgttgggtta acgccttctg
     2281 atgcatagtg tgcgaattca taaatttcga atccaacaaa gactaaacct aaaagtaacg
     2341 taatgatcat ccaaaacatc attaactttt gtttttcttg gcgcatgtag taaatagcaa
     2401 taccacatgt gtaagaactg aataataatg caaacgtcat tattaaaaca agaggcaatt
     2461 caaataactc agtagtcatt ttacctgcat aatcgccacc atgttgcaaa gttaatagtg
     2521 tcgcaaatag ggtaccgaat aacgcaaatt cggctgtaat gaaaatccaa aagccaagct
     2581 tatttaattc gccttcatgt gtgcgtgaat caatagtgtt tgtatcatga ctcatgactt
     2641 acagcctccc tttctttaat acgcgcttct cttaatctag cttcagtttc tgcaacttca
     2701 gcagcaggga tatgatatcc gtgatcgatt tggaaactgc gataaatcat agtaccaaaa
     2761 ataccgaata aacaaattaa tgctggaatt acagtttcga aaattaagaa gaaaccgccg
     2821 atagtcataa agataccaat ccagaatcca acaggagtat tgtttggcat atgaatgtct
     2881 ttgtaattat ggttgtctaa gtaatgacga ccatgttctt tcatatcaac aaatgtgtcg
     2941 tagtcattcc aatctggtgt aatggcaaag ttgtatttag gtggaattgc tgatgcagta
     3001 gtccactcta gagtacgacc aaggccatcc cagttatctc cagttgcttc acgtggagat
     3061 ttgaagtgac tgtatacgat actaacaaca aggaataaga atccgattgc cattaataat
     3121 gcaccgatag ttgaaattaa gtttaataag aaccaaccat ctgatggcat ataagtgtat
     3181 aaacgacgtg gcataccatc taatccaaga atgaattgtg gtaagaaaca aacgttaaat
     3241 ccgatcatga agaaccagaa gcaccatttg tttaatgttt cgtttaattt gtaacccatc
     3301 atctttggat accagaagat taaaccagct aagcaggcaa atacaacacc agtaaccaat
     3361 gtatagtgga agtgagctac taagaagtac gtattgtgat attgatagtc agctgatgcc
     3421 attgctaaca ttacacccgt aacaccacct aaaaggaagt tagggataaa tgctaatgag
     3481 aatagcattg gtgactcaaa tgtaatacgt cctttatata atgttaataa ccagttaaac
     3541 aatttcacac cagttggaat accaatcagc attgttgaaa ttgagaagaa tgagttgatt
     3601 aacgcaccat tacccattgt gaagaaatgg tgaacccaaa ctaagaaact aaggaacgcg
     3661 atgccggcag ttgcccatac catactttga tgtccgaata aacgcttacg agcgaatgtc
     3721 gggataattt ctgagtaaat accaaatgct ggaaggataa cgatataaac ttcagggtgc
     3781 ccccataccc agaagaagtt agcccaaagc attggcatac cgccatgtgc aactgtgaag
     3841 aatgctgtgt caaatattct atcagttgtc attaatgcta acgctactgt taaaggaggg
     3901 aaagcaagaa taacaattaa tgtagtaata aatgttgtta ctgtaaacat tggcatttgc
     3961 ataaacttca tagttggtgt tttacatctt aaaattgtta caaagaagtt gatacctgta
     4021 gctaaggtac caagccctga aatttgtata gctattaagt aatagttaac acccggacca
     4081 ggactgaatt cacctgctag tggcgcatag tttgtccaac cagctgctgg tgaaccacca
     4141 ataataaatg acaggttgaa taaaatcata cctgcaaaga atagccagaa acttacgttg
     4201 tttaatactg ggaatgcaac atcacgtgct ccaatttgta atggaacaac gatattccat
     4261 aaaccaaaga taaatggcat tgccatgaag ataatcatga ttacaccatg tgtactaaaa
     4321 atttcgttat agtggttaga ttctaaaaat ttgttatcag gtactgttaa ttgcgcacga
     4381 ataagtaacg catcaatacc accacggacg aacattaata cggcacagat taaatacata
     4441 ataccgattt tcttatggtc tacagatgtg aaccattctt tgtaaagata tttccataat
     4501 ttaaagtaag taattactgc gattaaacca ataactaaga atggggcacc aatttgtgcc
     4561 attgtaatca tccagttacc tttaactagt aattgatccc atggaaaatt cattaatgtc
     4621 cacctccatg atcatcattg tcttgatctt gcgcatcttt tgaaattttc ttcatttctt
     4681 tcgcattttt cgattcatct ttcttgaact cattgttata tggttcgtca tttccaagaa
     4741 tcatcaactt cataccatgt cgtttatagt tcgcatttgt aatttgagct ttacgagcag
     4801 gtattaatgg tttgtctgat acatctttaa acatattttc ttcactagtg aagtttggat
     4861 cttttaattc gaaattgaaa cgtttatatg catagaagat gtattctgga tcggctgctg
     4921 gatcaacaaa cgccatatgt gtaccattaa attctaaagc tttattaggt gtgcttggta
     4981 ataattgttt atcaaatgta tcttgatcta acgttttctt acctttaact tctttcaccc
     5041 atttgtcgta gtctttttga ctaacggcat ttactttaaa tgtttgacgt gagaatcctt
     5101 caccattgaa gttagagtta cgacctctga acgtaccagt ttgagatgct tctaacgtcc
     5161 aattcattgt catgccagtc atggcatatt tttgaccacc taattgtgga atccagaaac
     5221 ttgtcattgt atccatagct tgaagcttaa atacaacagg acgatcttta gggattgtta
     5281 atgtattaac agtctctata tgttcatctg gataagcaaa gaaccatttg tatcctgcac
     5341 ttactgcata tacaaccatt ggatctttct cactcttcgg tggtttttcg taatcgtata
     5401 aagttttaac tgtaggaata gctaaagcag caacgattat gataggtatt acaaaccata
     5461 ttgtttcaat gatggcatta tggtgcatct taccagattc ggcattctta ttataactat
     5521 acttgtaaat aaaaatggcg aacatgccaa gtacaacgaa acaaataaca agcatgaaga
     5581 cgattgaata aagaatcaag aacttctgac tacttgctac tggccctttt gcgttgaaaa
     5641 tttctatatt tgaacaacca ctaagtaaaa ttagtgtgcc aaataataga agcaaagact
     5701 taaattttga cacttttttg acctcctaat actacaaatg tagggcttaa cattaatttt
     5761 aagttattac acaatattta caagggctta tgggaaaaaa attaataaaa ttgtatcaaa
     5821 aatgttgata aatcaaggtg tgacgtgggt tcacacattt gttaaaatta tgtgtacatt
     5881 ttgtgactaa tagcgttttt gaaaaaattg cttcatattt aaaaggaaag ttgagaatag
     5941 ctttcaatta aaggttaatt gaatgaaaat aaaaaaagtt tgaaattgtc taataaattg
     6001 aaaatgatgg ctagttgatt gaaaggaagc gcaaacattt taataaggaa tttgccaaaa
     6061 gtaatttatc gttaaacaat gaaaagtgga aatttgtttg ttcaaaatca aaacattttt
     6121 ttaaaaagag cagttaagtt ttaataattt gataggtagt cctttggtat acagaaaaaa
     6181 tacaatattt gggttaaagt tgcagatgaa gttagaataa caaataaaat ggtagcaaaa
     6241 catgttcgtt tgctaccatt ttatttgcag tatagtgatg agggctattt aacctcaata
     6301 tacctagtgg aaattttaat tgcaccatct gaatcagtga catgatattt aatttgatat
     6361 ttaccagatt tagatgtatc aatttggcca tcgactttaa ttttatcggt taaatctcca
     6421 tcttctttat caaatgcact tatgccgttt agaaggttat aatcttgacc tttcttaata
     6481 acgatatcat tagcgccttt aatttgtggt gtagtattcg tcgttgcatc tgcatttaaa
     6541 tttggtgtta ctagtgtagc agaaacaccg agggctgata atgactgtaa tagtttattc
     6601 ataatgtaac ctccgaatga taagtcattt ctgagttagc tttactatac cttaatttat
     6661 attatttaca catcatacta tagtcctaat cttaccctat aaagaatgaa atttaaatat
     6721 aaatttataa ataatg
//
LOCUS       DALAAR010000112         6702 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000112 DALAAR010000000
VERSION     DALAAR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.116"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            155..1384
                     /locus_tag="O2458_002543"
     CDS             155..1384
                     /locus_tag="O2458_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCZ3888386.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1579..2436
                     /locus_tag="O2458_002544"
     CDS             1579..2436
                     /locus_tag="O2458_002544"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCZ3888387.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2436..3380
                     /locus_tag="O2458_002545"
     CDS             2436..3380
                     /locus_tag="O2458_002545"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCZ3888388.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3649..4617
                     /gene="pfkA"
                     /locus_tag="O2458_002546"
     CDS             3649..4617
                     /gene="pfkA"
                     /locus_tag="O2458_002546"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCZ3888389.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4639..6396
                     /gene="pyk"
                     /locus_tag="O2458_002547"
     CDS             4639..6396
                     /gene="pyk"
                     /locus_tag="O2458_002547"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCZ3888390.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2373 a          897 c         1361 g         2071 t
ORIGIN      
        1 attattttat tatcgtttat cgctatttgt gttacataat caaaaccata aattcttact
       61 cattcagatt tacccaatat ttttactttt ataatgtaat gcgttttatc caagttattt
      121 tttaaaaata aatattgaat taggggctga tttcatgtca ttaagagatg aagcattgga
      181 aatgcacaaa cgtaatcaag gtaaattaga agttaaacca aatgtaaaag ttactaataa
      241 agaggaatta agtttagcat actcacctgg cgttgctgaa ccgtgtaaag atatttatga
      301 agataaaaga aaagtatatg attacacaat taaaggaaat acagttgcag ttattactga
      361 tggaacagcg gtattaggtt taggtaacat tggacctgaa gcaagtattc ctgtaatgga
      421 aggtaaagca gtattattca aaagcttcgc tggtatcaat ggggtgccta ttgcgttaaa
      481 tacaactgat accgaagaaa tcattaaaac agttaagttg ttagaaccta attatggtgg
      541 tattaattta gaggatattt cggcaccacg ttgttttgaa attgaagaac gattgaaaaa
      601 agaaactaat attccggtat tccatgacga tcaacatggt acagcaattg taacattggc
      661 aggtttggta aatgcattga gagttgttaa caaagatatt gctaaaataa aagttgtact
      721 aaatggtgct ggtgcagcag gaatagccat tgttaaatta ctatacgcgt atggtgtaag
      781 aaatatggtt atgtgtgact caagaggcgc aatttttgaa ggacgttcat atggtatgaa
      841 tcctacgaaa gatgttgtag caaaatggac aaataaagat aagattgaag ggtctttaga
      901 agaagtcgta aaagatgcag atgtatttat cggggtttct gtagctaatg cgctgtcaca
      961 agatatggtt aagagtatgg cagataatcc aattatattt gcaatggcta atccaaatcc
     1021 tgaaataata cctgatgatg ccaaagcggc aggtgcacga gttgttggta caggacgttc
     1081 agactatcct aaccaaatta ataatgtatt agctttccct ggtattttta gaggtgcatt
     1141 agaggttgaa gctacacata taaatgaaga aatgaaaaag gcagctgtag aagcgattgc
     1201 tgatttaatc gatagttctg aattaaatga agactactgt atcccaggac cgtttgataa
     1261 acgtgtagcg ccatcagttg ctcgtaatgt tgctaaagcg gcaatggaat ctggagtagc
     1321 taggattgaa gttgatccgc aagatgtgta tgataaaaca atgaaactta cagatttaca
     1381 ataaaaataa ttaacgttta attaaattgc ttaaaagtta gcaataaaga gtcattaaaa
     1441 tgatgaaaat gaaagtttat gataaacatt caacagtcaa acgaatataa atcaaataaa
     1501 tttaaacccg tttttaactg gtcaagttca gtttaaggct ctaaatggtt agaacagagg
     1561 ttatttggag gttttcctat gtttaaagat ttttttaatc gaacaaagaa aaagaaatat
     1621 cttacagtac aagactctaa aaataatgat gtgcctgcag gtattatgac taagtgtcca
     1681 aagtgtaaga aaattatgta cacaaaagaa ttagctgaaa atttaaatgt gtgctttaat
     1741 tgtgatcatc atattgcttt aactgcgtat aaacgtatag aagcaatttc tgatgaagga
     1801 tcatttacag aattcgataa gggaatgacc tctgcgaatc cattagattt tccaagttat
     1861 ttagaaaaaa ttgaaaagga ccaacaaaag acaggtctta aagaagcagt tgtgactggt
     1921 acagcacaac tagatggtat gaaatttggc gttgctgtca tggattcacg ttttagaatg
     1981 ggaagtatgg gatcggttat cggtgaaaag atatgtcgca tcattgatta ctgcactgag
     2041 aaccgtttac catttattct tttctctgca agtggtggtg cacgtatgca agaaggtatt
     2101 atttccttga tgcaaatggg taaaaccagt gtatctttaa aacgtcattc tgacgctgga
     2161 ctattatata tatcatattt aacacatcca actactggtg gtgtatctgc aagttttgca
     2221 tcagttggtg atataaattt aagtgagcca aaagcgttga taggttttgc aggtcgtcga
     2281 gttattgaac agacaataaa cgaaaaattg ccagatgatt tccaaactgc agaattttta
     2341 ttagagcatg gacaattgga taaagttgta catcgtaatg atatgcgtca aacattgtct
     2401 gaaattctaa aaatccatca agaggtgact aaataatgtt agattttgaa aaaccacttt
     2461 ttgaaattcg aaataaaatt gaatctttaa aagaatctca agataaaaat gatgtggatt
     2521 tacaagaaga aattgacatg cttgaagcgt cattggaacg agaaactaaa aaaatatata
     2581 caaatctaaa accatgggat cgtgtgcaaa ttgcgcgttt gcaagaaaga cctacgaccc
     2641 tagattatat tccatatatc tttgattcgt ttatggaact acatggtgat cgtaatttta
     2701 gagatgatcc agcaatgatt ggtggtattg gctttttaaa tggtcgtgct gttacagtta
     2761 ttggacaaca acgtggaaaa gatacaaaag ataatattta tcgaaatttt ggtatggcgc
     2821 atccagaagg ttatcgaaaa gcattacgtt taatgaaaca agctgaaaaa ttcaatcgtc
     2881 ctatctttac atttatagat acaaaaggtg catatcctgg taaagctgct gaagaacgtg
     2941 gacaaagtga atctatcgca acaaatttga ttgagatggc ttcattaaaa gtaccagtta
     3001 ttgcgattgt cattggtgaa ggtggcagtg gaggtgctct aggtattggt attgccaata
     3061 aagtattgat gttagagaat agtacttact ctgttatatc tcctgaaggt gcagcggcat
     3121 tattatggaa agacagtaat ttggctaaaa ttgcagctga aacaatgaaa attactgccc
     3181 atgatattaa gcaattaggt attatagatg atgtcatttc tgaaccactt ggcggtgcac
     3241 ataaagatat tgaacagcaa gctttagcta ttaaatcagc gtttgttgca cagttagatt
     3301 cacttgagtc attatcacgt gatgaaattg ctaatgatcg ctttgaaaaa ttcagaaata
     3361 tcggttctta tatagaataa tcaacttgag catttttatg ttaaatcgat actgggtttt
     3421 accataaatt gaagtacatt aaaacaataa tttaatattt agatactgaa ttttaactaa
     3481 gattagtagt caaaattgtg gctactaatc tttttttaat taagttaaaa taaaattcaa
     3541 tatttaaaac gtttacatca attcaataca ttagttttga tggaatgaca tatcaatttg
     3601 tggtaattta gagttaaaga taaatcagtt atagaaaggt atgtcgtcat gaagaaaatt
     3661 gcagttttaa ctagtggtgg agattcacct ggaatgaatg ctgccgtaag agcagttgtt
     3721 cgtacagcaa tttacaatga aattgaagtt tatggtgtgt atcatggtta ccaaggattg
     3781 ttaaatgatg atattcataa acttgaatta ggatcagttg gggatacgat tcagcgtgga
     3841 ggtacattct tgtattcagc aagatgtcca gagtttaagg agcaagaagt acgtaaagtt
     3901 gcaatcgaaa acttacgtaa aagagggatt gagggccttg tagttattgg tggtgacggt
     3961 agttatcgcg gtgcacaacg catcagtgag gaatgtaaag aaattcaaac tatcggtatt
     4021 cctggtacga ttgacaatga tatcaatggt actgatttta caattggatt tgacacagca
     4081 ttaaatacga ttattggctt agtcgacaaa attagagata ctgcgtcaag tcacgcacga
     4141 acatttatca ttgaagcaat gggccgtgat tgtggagatc tagcattatg ggctggatta
     4201 tcagttggtg ctgagacaat tgtagttcca gaagtgaaaa cagatattaa agaaatagct
     4261 gataaaattg aacaaggtat taaacgtggt aagaaacact caatcgttct tgtagcagaa
     4321 ggttgtatga ctgcgcaaga ttgtcaaaaa gaattatcac aatacatcaa tgttgataat
     4381 agagtgtctg tgttaggtca cgttcaacgt ggtggtagcc caacaggtgc ggatagagtt
     4441 ttagcatcac gtttaggtgg atatgcggta gacttattaa tgcaaggtga aacagctaag
     4501 ggtgttggaa ttaagaacaa taaaattgta gcaacatctt ttgatgaaat ttttgatggt
     4561 aaagatcata aatttgatta tagtctatat gaacttgcta acaagttatc tatataagat
     4621 ttcaggagga attataaaat gagaaaaact aaaattgtat gtacaattgg accagcttca
     4681 gaatcagaag aaatgattga gaaattaatc aatgctggta tgaacgttgc acgattaaac
     4741 ttttcacatg gtagtcatga agagcataaa ggtagaattg atacaattcg taaagtagct
     4801 aaaagattag acaaaattgt agcaatttta ttagatacaa aaggtccaga aattcgtacg
     4861 cataatatga aagacggtat cattgaactt gaacgtggca acgaagttat tgttagcatg
     4921 aatgaagttg aaggaacacc tgaaaagttc tcagtaacat atgaaaactt aattaacgat
     4981 gttcaagtag gttcatacat tttacttgat gatggcttaa ttgaattaca agttaaagat
     5041 attgaccatg ctaaaaaaga agttaaatgt gatattttaa actctggtga gcttaaaaac
     5101 aaaaaaggtg ttaacttacc tggcgtaaga gtaagtttac ctggtattac agaaaaagat
     5161 gctgaagata tccgtttcgg tattaaagaa aatgttgact tcattgcagc aagtttcgta
     5221 cgtcgtccta gtgatgtttt agaaattcgt gaaattttag aagaacaaaa agctaacatt
     5281 tcagtattcc ctaaaattga aaaccaagaa ggtattgata atattgcgga aattcttgaa
     5341 gtgtctgatg gtttaatggt tgcacgtggt gacatgggtg ttgaaattcc acctgaaaaa
     5401 gtaccaatgg ttcaaaaaga tttaatcaga caatgtaaca aattaggtaa accagttatt
     5461 acagctacac aaatgttaga ttctatgcaa cgtaacccac gtgctacacg tgcagaagct
     5521 agtgacgttg ccaacgcaat ctatgatggt acagatgcag taatgttatc tggtgaaact
     5581 gctgctggtt tatatcctga agaagctgtt aaaacaatga gaaatattgc tgtatcagct
     5641 gaagcagccc aagattacaa aaagttattg tcagatcgta ctaaattagt tgaaacttca
     5701 ttagtgaatg ctatcggtat ttcggttgca catacagctt taaacttaaa tgttaaagca
     5761 attgtagctg ctactgaaag tggttcaacg gcacgtacta tctccaaata tcgtccacat
     5821 tcagacatta ttgcggtgac tccaagtgaa gaaactgcac gtcaatgttc aattgtttgg
     5881 ggagttcaac ctgtagttaa aaaaggacgt aagagtacag atgcattgtt aaacaatgca
     5941 gttgcaacag ctgttgaaac tggtagagta tctaatggtg atttaatcat tattactgct
     6001 ggtgtaccaa ctggtgaaac tggaactact aatatgatga aaatccacct agttggtgac
     6061 gaaattgcta atggtcaagg tattggacgt ggatcagttg ttggtactac gttagttgct
     6121 gaaactgtta aagatttaga aggtaaagat ttatctgaca aagttatcgt tactaactca
     6181 atcgatgaaa cgtttgtacc ttatgtagaa aaagctttag gcttaattac agaagaaaat
     6241 ggtattacat caccaagtgc aattgttggt ttagaaaaag gtattccaac agttgtaggt
     6301 gtagaaaaag ctgttaaaaa cataagcaat aacatgttag ttacgattga tgctgctcaa
     6361 ggtaaaatct ttgaaggata tgcaaacgta ctataattta taaaaaaacg tctttccatt
     6421 tatcaacaat ggaaaggcgt tttttggttc atctggtatt ttatgacgta attaataggt
     6481 tatttgataa tgatagtgta tgaatggcaa tctatataaa tgtttatatc ttttatacat
     6541 gtacattatc accttcaaac tttcactcat attactttgg aaatttatta taaaatagaa
     6601 gtatggatgt atttctgaaa tgatacatta ttaaatagat gagaaagtaa aagttttgag
     6661 ccaagtacgc aatttaatat tataagttgc atataaaaca gg
//
LOCUS       DALAAR010000113         6039 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000113 DALAAR010000000
VERSION     DALAAR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.118"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..432
                     /locus_tag="O2458_002548"
     CDS             <1..432
                     /locus_tag="O2458_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCZ3888391.1"
                     /translation="REDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDST
                     KYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLG
                     TAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            738..1511
                     /locus_tag="O2458_002549"
     CDS             738..1511
                     /locus_tag="O2458_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888392.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            1623..2407
                     /locus_tag="O2458_002550"
                     /pseudo
     CDS             1623..2407
                     /locus_tag="O2458_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2404..3728
                     /locus_tag="O2458_002551"
                     /pseudo
     CDS             2404..3728
                     /locus_tag="O2458_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3728..4054
                     /locus_tag="O2458_002552"
     CDS             3728..4054
                     /locus_tag="O2458_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888393.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            4057..4344
                     /locus_tag="O2458_002553"
     CDS             4057..4344
                     /locus_tag="O2458_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888394.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            4661..4768
                     /locus_tag="O2458_002554"
                     /pseudo
     CDS             4661..4768
                     /locus_tag="O2458_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            4880..5674
                     /locus_tag="O2458_002555"
     CDS             4880..5674
                     /locus_tag="O2458_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888395.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(5678..5800)
                     /locus_tag="O2458_002556"
     CDS             complement(5678..5800)
                     /locus_tag="O2458_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888396.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT         2481 a          742 c         1089 g         1727 t
ORIGIN      
        1 agagaagatt ttagtaaaat atttaattgg aataaaaatg gttcgtctgt aatcatggga
       61 tacatgataa aaagccaaga aatgccaata tttatcactt atgataagca tgaggatata
      121 agtgatagca caaaatatga agatgaattt ttaagtcaag atgaattaaa atggtttact
      181 aaatctaata gaacgctaga atcaaaagaa gttcagaaaa ttttgtccca tagagcaaaa
      241 ggaattaaaa tgtatatttt tgtacagaaa aaagatgatg atggtatata tttttattat
      301 ttaggaactg ctggatatat agaaggttca gaaaagcaag ataaaatgcc gaatggttca
      361 aacgtagtaa cgatggattt agcactggat aaagctgtaa gagatgatat atatcgttat
      421 ctaacaaatt aatgtaaacc gaaaaatgaa tgttagtaaa gttgtattta aacatcaatt
      481 ttaataagta aatacattga gtttcagtgt tattatttta aatataagcg caatttatct
      541 taattaatta gttggttagg ccaataaaaa ttataaagat atttgcgctt tttaaataat
      601 ttgtgatgtt gatatgtgaa aaatacaact aacaattaaa atccacgagg atttaatcat
      661 aaaatttcct ctaatattat taatattgga atttgaactt aactttgcat caaaaataaa
      721 aatggggatg tgaaatgatg attcactcaa aaaggttgag gctctggctg tatttagtat
      781 tattagctgt ttttataggg gcttgcggaa tgaaaaagga agaaagcagt aaagataaac
      841 aaattaaaga aaacttcaac aaaacgttga gcctgtatcc aactaaaaat cttgaagact
      901 tttatgataa ggaaggcttt cgagatcaag agtttgaaaa gggcgacaaa ggcacttgga
      961 taattcactc taaaatgaca atagaaacaa atggaaagaa tatggaatcc agaggattgg
     1021 tcctgtatgt agaccgcaat accagaacga caaaaggtga atttattgtg agggaattat
     1081 gggaagataa aaaaggatac tcacgtagta aagaaaaaga atatccagta aaaatggaac
     1141 ataataaaat tatcccaacc aaaccgatag ctgatgataa attaagaaaa gaaatcgaaa
     1201 actttaaatt ctttgtacaa tacggtgact ttaaagatat taatgattat aaagatggtg
     1261 acatttcata taatccgaat gtgccaagtt attcagcaaa gtatcaatta agtaatgatg
     1321 actacaatgt gaagcaactt agaaagaggt atgatatacc aactaagaaa gcacctaaat
     1381 tattaataaa aggcgatggt gatttaaaag gatcatctat aggacacaaa aatttagagt
     1441 ttacatttat agaaaataaa gaagaaaaca tctattttac ggatagtatt aatttcaaac
     1501 ctactgaata gggttaatag aagtttgaaa tgaaaatttc ataaagtgtt caaaataatt
     1561 tttcgcttta gttgtgtaaa ttaaagattg agtcagtatt aaaaatataa ggtggagaaa
     1621 tgatgattca ttcaaagaaa ttaacgctcg gtatatgttt ggtattactc attatattga
     1681 ttggaggttg tgtcattatg acaaaaacaa atggtcgaaa cgctcaaatt aaagaaaatt
     1741 tcaacaaaac attaagtgta tatccaacca agaatctcga tgatttttac gataaggaag
     1801 gttttcgaga tcaagaattt gataaaagag ataaagggac ttggattatt tattctgaaa
     1861 tggttatcga accaaaaggg aaaaatatgg aatcgagagg aatggtgctc tatatcaatc
     1921 gcaatactag aacaacgaag ggtaatttta ttgtcaccga aataactgaa gatagtaaag
     1981 gatattcacg tagtaaagaa aaaaatatcc tgtcaagatg gaaaataatc gaattattcc
     2041 aacaaagcct ataccggatg acaagttaaa aaaagagatt gaaaacttta agttctttgt
     2101 acaatatgga aattttaaag attttaaaga ttataaaaat ggtgatattt catataatcc
     2161 taatgtgcca agttattctg caaagtatca attgaataat gatgactata atgttcaaca
     2221 gttaagaaaa cgatatcata ttccaaccaa acaagcgccc gaattaaaat tgaaaggatc
     2281 cggcaattta aaaggctcat ccgtaggatc taaggatcta gaatttacgt ttgtagaaaa
     2341 tcaagaagag aatatctatt tttcagattc ggtcgaattt acacctagcg aggatgataa
     2401 atcatgagtc aaacggaata tcaaataaaa tctggcaata taaaaggtaa ctctgaagaa
     2461 acaagtacag tatctaatat aagttatgaa atagaaaacg caaataacag tggtttaaaa
     2521 caaaataaaa ttgataaaca aattaaaaag ttacaagaaa aaaataaatt ccctaaaaat
     2581 ctttcatatc ttaaaagtta tacggacccc aaaacaggca cgactacaag cgccttttta
     2641 aataaagaca ctggcaaagt tactttaggt atgacaggta ctaatgtaca caaagacgca
     2701 atattaaaac aaacatttgg tgttccttct tatcaaggat atatagatgt gagtgaaacg
     2761 ctaaaggata ttggggccga tgtcaatatt ggccttcatt ccgtcacaga taaagatcca
     2821 cattataaaa atacccaaga ctttatcaaa aatatcaaaa aagactatga tattgatatt
     2881 attaccggac attcgctggg cggtagagat gcgatgattt taggtatgag taatgatatt
     2941 aaacatatcg ttgtgtataa tccagctcca ttagcgatca aagatgtgag cggcctttat
     3001 gccgatcaag aagaacttaa aaaattgatt gaaaagtacg atggtcacat tgtaagattt
     3061 gtgtctgatg aagacgaatt agatgcaggt gtccgcaatc atttatatga aactgctgga
     3121 gaaaaaatag tacttaaaaa tggagaaggc catgcaatga gtggtatttt aatgagcaga
     3181 acacaggcta taatcttagc tgaattaaac aaagttaaag gctaccaaga cgaaaataat
     3241 aaagcattaa aatccgttcg taaacaaacg aggcatagat tacataaagt agagacgtta
     3301 agagcgaatt ggattcaaac aacgggtgga tcactctctt cctcccaaca acaattatta
     3361 gaagctttaa cagcactaac cattgccgaa ggcttaaatc aattagtgaa tgaagaaagc
     3421 caacatttga aaaaatgtat cacgcgatgg cacataaatt tggagacaac tggaaaaaag
     3481 cgcaagaagt tggaaatgaa attggtgaaa aattaacctc tgaagaggtt atagatgaat
     3541 taagaaaagg tggcgcgtat gaaagtaaac ttgaaacaga tcccaaaaga aaaattgatg
     3601 ataagataaa gaaattaaat gatgtttata aaaattgtaa tggctatatc gcaaaaatta
     3661 aacagagtat cgaagcaatt gtttctaatg accaaatgtt agcgagccag attgatggga
     3721 tgatgtaatg tttactacgt ataaaaatat taatgaactt gaaaatgcct atgatgaaga
     3781 aagaaaacaa ttgaatgatg cattcaatca aattgatgaa ttaagacatc aaacacgcaa
     3841 gaaatgtgaa caaatgtatg atcatttctt atatctcaaa cataaaatga attatagtga
     3901 agacgctatg atcaggatga cacgtattat agaatctttc gatagagaaa cgaatcaacg
     3961 tatccgacat cacgaaatga aattagaaga ttataaagat gagttaagaa gagaatatct
     4021 aaaacaatct gacagaattg aaggagatga ataagcgtgg gacttgattt tagtggttta
     4081 ccagatttag cagtattgga acaaatgaag gaaaaagaac agattagtga ggttattgcg
     4141 cctgaacatg ttcgtatgca tcatgatcat caaaataagc tgaaaagtga tgagaaaata
     4201 ttacttgacc aaatggtaag tcatttcaaa aaatttgaag atgattttaa aaatgcggca
     4261 caaggggctt gggtgaaaaa tgccacagac gaattaaaag atattagtaa tgatttagaa
     4321 aaaattcaag atattaaagt ataaaaaggt attaagaaac acaataagta tataatccat
     4381 ttaataataa atgaattata tagttcataa tttcgactat aagtggctat tagccattat
     4441 ttttcgggat ctatgtcaaa tcggactaat gaattcaata atggaagtta agcaaccaat
     4501 ctttgtttaa cttctttttt attttttgga aaataaagtt ttgaacataa taaaatttga
     4561 ttatgtttta acgaattttg atgtttcttg aactatatat catctagtcg tcatttacaa
     4621 ttggtaaata tgacttcaaa ctgtatgaag gtgatggcga ttaaaaggct catccgtagg
     4681 ttctaaagaa ctagaattta actttataag aaataaagaa gaaaatgtgt atttttctga
     4741 tagtattaat ttcaaaccta ctgaataggg ttaatagaag tttgaaatga aaatttcata
     4801 aagtgttcaa aataattttt cgctttagtt gtgtaaatta aagattgagt cagtattaaa
     4861 aatataaggt ggagaaatga tgattcattc aaagaaatta acgctcggta tatgcttggt
     4921 attactcatt atattgattg taggttatgt cattatgaca aaaacaaatg gtcgaaacgc
     4981 ccaaattaaa gacacattta atcaaacatt aaaattatat ccaaccaaaa atctcgatga
     5041 tttttacgat aaagaaggct ttcgagatca agaatttaaa aagggtgata aaggtacttg
     5101 gatagttaat tctgaaatgg taatcgagcc aaaaggtaag gatatggaaa cgagaggaat
     5161 ggtgctctat atcaatcgca atactagaac cacaaaaggg tattatttta taagtgaaat
     5221 gacagatgac agtaacggca gaccaaagga tgatgaaaaa aggtatccgg taaaaatgga
     5281 acataataaa atcataccaa cgaagccact accgaatgac aagttaaaaa aagagattga
     5341 aaactttaag ttctttgtac aatatggcaa ctttaaagat attaatgatt ataaagatgg
     5401 tgatatttca tataatccta atgtaccaag ttattcggca aaatatcaat tgaataatga
     5461 tgattataat gtccaacagt taagaaaaag atatgatatt ccaaccaaac aagcgccgaa
     5521 actattattg aaaggcgatg gagatttaaa aggttcatcc gtaggttcta gaagtcttga
     5581 atttaccttt gtcgaaaata aagaagaaaa catttatttt acagattctg tacaatatac
     5641 tccaagtgag gatacaaggt atgagtcaaa ctgaatatca aataaaacct ggtaatataa
     5701 caagtaactc tgaagaaaca agttcgatat ctaaagtgag ctgtgaaata taggtagcca
     5761 tttcaaaaaa tttaaaggtg aatttgataa tgtagctcaa ggagattggg ttaaaaaggc
     5821 gaagaatgaa ctggatgata ttagtaagaa attaaaaaat attcaaagaa cggaagttta
     5881 atagcttata tgattcttgg agctaagaca gcatgcgttc attcatgcca ttattaatat
     5941 aagcaccgca acaaaaaagc ttctaatgtg atacaggaac ctcatattcc gtatcatgtt
     6001 agaagctttt aatgtctaaa gaacatctac attttatca
//
LOCUS       DALAAR010000114         5919 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000114 DALAAR010000000
VERSION     DALAAR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.119"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            103..451
                     /locus_tag="O2458_002557"
                     /pseudo
     CDS             103..451
                     /locus_tag="O2458_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="O2458_002558"
     CDS             462..656
                     /locus_tag="O2458_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888397.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            697..1047
                     /locus_tag="O2458_002559"
     CDS             697..1047
                     /locus_tag="O2458_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888398.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1054..1245
                     /locus_tag="O2458_002560"
     CDS             1054..1245
                     /locus_tag="O2458_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCZ3888399.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(1794..2819)
                     /locus_tag="O2458_002561"
     CDS             complement(1794..2819)
                     /locus_tag="O2458_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCZ3888400.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            3089..3286
                     /locus_tag="O2458_002562"
     CDS             3089..3286
                     /locus_tag="O2458_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888401.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            3344..4825
                     /gene="cls"
                     /locus_tag="O2458_002563"
     CDS             3344..4825
                     /gene="cls"
                     /locus_tag="O2458_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ3888402.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            5000..5908
                     /locus_tag="O2458_002564"
     CDS             5000..5908
                     /locus_tag="O2458_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888403.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
BASE COUNT         2260 a          788 c          898 g         1973 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcatcttta ttaccaagtt atactataat agattctaat
      841 gaaagtgaaa agatgactaa aaaagaattt attgatattc cagttttgtt tgatgatgaa
      901 gggaatttta gaataaaaca agttatagat tataaaaaga taataggtaa atcttatgtg
      961 aatggtaaat atattgaaac gaaattaggt aaggttcatt attctaaaac tggttttcat
     1021 gtagttccgt atattaaaaa ggagtgatta aatatgaatt tacagtcata cataggcaaa
     1081 ttagtcaaat taacgcttac taataataaa atattaattg gaaaagtgat agactttgat
     1141 gataaagttg ataattttga tggttataat tcgatagaaa ttgatacagg tagaattaca
     1201 tatgatatat cagaaaataa aattaaaact attgttttac aataatagca tcctttctac
     1261 acagataaag agaaagcggt gctatttttt gcgcttttat aacaactaag gatacaagac
     1321 acataacttc aacaatatcc tcatctaaac ctatcgattt cctaagctca attttaaaca
     1381 caaagtttat ttgaaatgat gcacacctat aaaaatctta atcatcgaaa agtcacaaac
     1441 atctccatat taaaaactta aatagtgcat tccatttaaa ctaaacatcg atattcctac
     1501 caatatagac ttagtaattc gattgttaat acattacttt cataaaataa ctacaaccat
     1561 cataatactt ctcaaatgtc gtcaaattaa agtaaataca gcacaaatta acatttttaa
     1621 atatataaaa taaaacaaga tactcaataa tctgtagtac cttgttttgg aataatgtaa
     1681 ttttatatca tcaacggcta caaatgagca gtttcatata taaataaaca catctactca
     1741 actgatctta attaattaat aaatgattta ccagatgaca tataacagca ttattagatt
     1801 aattctaata gtttatttaa attttcttcg gttgtcgccc aactggttgc gaatctaaca
     1861 acacgatgtt gatcatcgta tttttcccaa acagcaaatt taactttttg ttctaactct
     1921 gctattttct cgttacttaa aataaaaaat tgttgattgg ttggagaatc aaagtaaaga
     1981 cgatagcctt tatttttaaa cccgtctttc atcttatttg ccatttcgat agcatgtctg
     2041 cttatattaa aatataaatt gtccgtaaat aattctaaaa attgtatgcc tgttaaccgt
     2101 ccttttgcta aaagggcacc gtgatgcttg attcgagtgg taaattgttt cggttcatta
     2161 tttttcgtaa aaacaatggc ttccccgcat aatgcaccta tcttcgtacc acctatataa
     2221 aatacatcac aatatttagc gatgtcttta atagtcatat ctgattggtc actcatcaat
     2281 ccatacccta atcgtgcacc atccataaat aatggaagct gatattgctt acatactttg
     2341 cataactctt ccaattctga tttagagtat aatgtgccat attctgtagg atgagaaata
     2401 tataccattc ctgggaatac catatggtcc tttttaaaat cacttttaaa tgtctccatg
     2461 taagtttcaa catctgaagc actaactttt ccttccttag agggtatagt aattacttta
     2521 tgtccactat attcaattgc accgccctca tgcacagcaa catgaccagt gtctgctgaa
     2581 atgacccctt cgtaactttc taacattgaa ttaataacaa cctgattggt ttgtgttcca
     2641 cctactaaaa aacgaattgt agcatttgga cagtcaattg tatctttaat cttttcaatt
     2701 gcctgagctg tgaattgatc aaagccatat cccgaagctt gtacaagatt tgtatctact
     2761 aatcgtttta atactttttc atgagcacct tctaaataat cattttcgaa tgaaatcact
     2821 acatttcccc ctaaaactaa tatcaacatt ttaataagat aaaccaattt caaaactagt
     2881 tcgatattta aaatgtatta tgaatggtta aagtttgtat cgcattatcg cgaagttgaa
     2941 taaatatatt atacatcaaa caatacatta tgaaaattaa ttaatttttt atcacagcga
     3001 cttttcatgc tggaacacat gtattaatga ttgaatttta ctgttaaaag ttctatgatt
     3061 taggaaatag agaaaagagg ggatttaaat ggaacaaatt aaacttaaaa cttttacagc
     3121 tgagacttta gagttattag aaaaaaacat taatgctttt ttaagttctg aagaagctac
     3181 aaatttaaaa ttagtaaata ttactattaa agaaatagaa gaaagaacat tcccaaataa
     3241 tgaagaagaa ttcaatgcaa ttttaacttt atctgtgaat aaataatatg acgtgaaagg
     3301 tgcaaaatgt ttttttgtgc ctttctttat aggagaaaag actatgcgat ttacattttc
     3361 aaacgattta ggaacgttat ttactattat tttagccatt ggattcatca ttaatttagt
     3421 attggctttt attattatct ttttagaaag aaataggcgt acagcgagtt caacttgggc
     3481 atggctattt gtactttttg tcttaccatt gattggtttt attctttact tgttttttgg
     3541 tagaaccgtt tcggcacgca aattgaataa aaacaatggt aacgtgttaa cggatttcga
     3601 tggactttta aaacaacaaa tagaaagctt tgataaaggt aattatggta ctgataacaa
     3661 acaagttcaa aaacatcatg atttagtacg tatgcttttg atggatcaag atggtttttt
     3721 aactgaaaat aataaagttg atcatttcat tgatggaaat gatttatatg atcaagtttt
     3781 aaaagatatt aaaaatgcaa aagaatatat ccatttagag tactatactt tcgctttaga
     3841 tggtttaggt aaaagaattt tacatgcttt agaagaaaaa ttgaaacaag gtctagaagt
     3901 aaaaatatta tatgatgatg ttggatctaa aaatgttaag atggcaaatt ttgatcattt
     3961 taaatcgtta ggtggagaag ttgaagcatt ttttgcttca aaattaccgt tattgaattt
     4021 cagaatgaat aatagaaatc atagaaaaat catcgtaatc gatggtcaac taggttatgt
     4081 cggaggattt aacattggtg atgaatatct aggattagga aaattaggat attggagaga
     4141 tacgcattta cgtatacaag gggatgcggt tgatgcactg cagttgcgat ttattttaga
     4201 ctggaattcg caagcgcacc gtccacaatt tgaatatgat gttaagtatt tccctaaaaa
     4261 gaacggacca ttgggcaatt caccaattca aatagctgca agtggcccgg ctagtgactg
     4321 gcatcaaatt gaatacggtt atacaaaaat gattatgagt gcaaagaaat ctgtatattt
     4381 acaatcacca tatttcattc cggataattc atatataaat gccattaaaa ttgctgctaa
     4441 atcaggtgta gatgtacatt taatgattcc atgtaagcca gatcatccat tagtatattg
     4501 ggcgacattt tcaaatgcct ctgacttatt atcaagtggt gttaaaattt atacgtatga
     4561 aaatggattt atacattcta aaatgtgctt aattgatgat gaaatcgtat cagtgggcac
     4621 agcaaatatg gactttagaa gttttgaatt aaattttgaa gtaaatgcct ttgtatatga
     4681 tgaaaatctt gctaaagatt taagggtggc ttatgaacat gatattacaa aatcaaaaca
     4741 actaaccaaa gaatcatatg ccaatagacc gctgtctgtt aaattcaaag aatcgttagc
     4801 aaaattagtt tcgccaattt tataatttat ttgtaaggag tctcgattat agaggctctt
     4861 tttattttgc ccaaaattaa aataacgata aatatattat gtaaatctaa taatgacatt
     4921 tgtcatttca aatctatgac aaagtgttct attttaaatc taataaattt actaatatta
     4981 aattaagaaa aaaatagagg tgattaaatt gattcaaata tctaatatca acaagtcatt
     5041 taataaaaga tgtgttctaa aaaatatttc gttcgatatt gaacaaggta aatgtatcgc
     5101 tttaattgga aaaaatggtg ctggaaagtc aacgttaatt gatatattaa ttggtaatgt
     5161 taatgctaat tctggtgaga tatttgataa agacaagtta ttacaaagtg aaaatcgcag
     5221 tataatgttc caaaaaacga tgtttccaga tcaattaaaa gttattgaga ttatcaactt
     5281 atatcaatca ttttacgaaa atccattacc attggaagaa ataatagaac tgacgaaatt
     5341 tgattctagt caactgaacc aatttgtaaa taaactttct ggtggtcaac aacgattact
     5401 cgattttgta ttatctttaa tcggacaacc acaattgatc ttattagatg aaccaacatc
     5461 gactatggat atagaaatta gagaatattt ttggtcaatt attgaaaatt taaaagaaga
     5521 taatcgaacg atactctata catcgcacta tattgaagaa gtcgaacgta tgtcagacaa
     5581 aattattctc attgaaaatg gagaaataat acttaatgat tcaacgtcac atattagaac
     5641 caatcagcaa tctcagatta cgttatccga tgaatatata agaaagttaa aactagataa
     5701 agatgattta gttattcaaa aaaatcataa tggcactatc aaaattatta cttcaaatgt
     5761 aaatgatacg attttatatc ttcaacaact tcatattaat ttggatgata ttgaaataca
     5821 aaaagtctca attgttgatt catacttcaa caataaaaag caaaggggat ctaattatga
     5881 tactaagtta cttgaaaatc gaatttaaag ttataatgc
//
LOCUS       DALAAR010000115         5881 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000115 DALAAR010000000
VERSION     DALAAR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.120"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            38..745
                     /gene="splA"
                     /locus_tag="O2458_002565"
     CDS             38..745
                     /gene="splA"
                     /locus_tag="O2458_002565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCZ3888404.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            870..1592
                     /gene="splB"
                     /locus_tag="O2458_002566"
     CDS             870..1592
                     /gene="splB"
                     /locus_tag="O2458_002566"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCZ3888405.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1650..2369
                     /gene="splC"
                     /locus_tag="O2458_002567"
     CDS             1650..2369
                     /gene="splC"
                     /locus_tag="O2458_002567"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCZ3888406.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2490..3209
                     /gene="splD"
                     /locus_tag="O2458_002568"
     CDS             2490..3209
                     /gene="splD"
                     /locus_tag="O2458_002568"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCZ3888407.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3158..3265)
                     /locus_tag="O2458_002569"
     CDS             complement(3158..3265)
                     /locus_tag="O2458_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888408.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3367..4083
                     /gene="splE"
                     /locus_tag="O2458_002570"
     CDS             3367..4083
                     /gene="splE"
                     /locus_tag="O2458_002570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCZ3888409.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4234..4953
                     /gene="splF"
                     /locus_tag="O2458_002571"
     CDS             4234..4953
                     /gene="splF"
                     /locus_tag="O2458_002571"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCZ3888410.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5018..5152
                     /locus_tag="O2458_002572"
     CDS             5018..5152
                     /locus_tag="O2458_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888411.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5316..>5881
                     /locus_tag="O2458_002573"
     CDS             5316..>5881
                     /locus_tag="O2458_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ3888412.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2288 a          864 c         1044 g         1685 t
ORIGIN      
        1 cattaactat aaaaataaat ttggaaggag gtaaaatatg aataaaaatg taatggttaa
       61 aggtttaact gctttaacta ttttaacatc tcttggtttt gctgaaaata tttctaatca
      121 gcctcattca attgccaaag cagaaaagaa tgtcaaagaa attaccgatg caactaagga
      181 accatacaat tcagtggtag catttgtggg tggtactggt gtagttgttg gtaaaaatac
      241 aatcgtaact aacaaacata tcgctaaaag taatgatatt tttaaaaata gagtatcagc
      301 acatcattcg agtaaaggta aaggcggagg aaactacgac gttaaagaca ttgtagaata
      361 tcccggaaaa gaagaccttg cgatagttca tgttcatgaa acaagtacag aaggtttgaa
      421 ttttaataag aacgttagtt atacaaaatt tgcagacgga gcaaaagtga aagatagaat
      481 ttctgttatt ggttatccaa agggtgcaca aacaaaatat aaaatgtttg aatcgacagg
      541 aacgattaac catatcagtg gaacgtttat ggaatttgat gcgtatgcac aaccaggtaa
      601 ttcaggatct cctgtattga attctaaaca tgaactgatt ggtattttat atgcaggtag
      661 tggaaaagat gaatctgaaa agaatttcgg tgtttatttc acaccacaat taaaagaatt
      721 tattcaaaat aatattgaaa aataagtatc atcaattcat tcgtgaagtt gattttttaa
      781 agagtgatta agaaaacggt tacataatta actaaatata ctgaattatg tatctagata
      841 tcaaaataat taaaagagag gaacttaaaa tgaacaaaaa cgtagtcatc aagagtttag
      901 cagcattaac aattttaaca tctgtaacag gtattggaac aacattggtt gaggaagtac
      961 aacaaactgc caaagcagaa aataatgtca caaaagttaa agatactaat atttttccat
     1021 atactggtgt agttgctttt aaaagtgcaa ctggatttgt agttggaaag aatactattt
     1081 taacaaataa acatgtgtcg aaaaattaca aagtgggcga tcgtattact gcacatccaa
     1141 atagtgataa aggtaatggt ggtatttatt cgattaaaaa gattattaat tatccaggta
     1201 aagaagatgt atcagtcatt caagttgaag agcgtgcaat agaacgtgga ccaaaaggct
     1261 ttaattttaa tgataatgta acgccattca aatatgcggc aggggctaaa gctggtgagc
     1321 gaattaaagt gattggttat ccacacccat acaaaaataa atatgtttta tatgagtcaa
     1381 ctggccctgt gatgtcagta gaaggtagca gtattgtata ttcagcgcat actgaaagcg
     1441 gaaactctgg atcacctgta ttaaacagca acaacgaatt agttggtatt cattttgctt
     1501 ctgatgtaaa aaatgatgat aacagaaatg catatggcgt ctactttaca ccagaaatta
     1561 aaaaattcat tgcagaaaac atagataaat aaacaaattg actttaaacg agcgttgcaa
     1621 catatctcga attgtaaagg agcttgaaaa tgaataaaaa tatagtcatt aaaagcatgg
     1681 cagcattagc cattctaacc tcagtaactg gaataaatgc tgcagtcgtt gaagagacac
     1741 aacaaatagc aaatgcagag aagaatgtta cgcaagttaa agatacaaat atttttccat
     1801 ataatggcgt cgtttcattt aaagatgcga caggttttgt aattggaaaa aatacaatta
     1861 tcaccaataa acatgtatca aaagattata aagttggcga tagaattact gcccatccaa
     1921 acggtgacaa aggaaatggt ggtatatata aaattaaaag catttctgat tatccgggtg
     1981 atgaagacat ctctgtcatg aatattgaag aacaagcagt cgaacgtgga ccaaaaggct
     2041 ttaattttaa tgaaaatgtc caagcattca attttgcgaa agatgctaaa gttgatgaca
     2101 aaattaaagt tattggttac ccattacctg ctcaaaatag ttttaaacag tttgaatcta
     2161 caggaactat aaaaagaatc aaagacaata ttttaaattt tgatgcatac attgaacccg
     2221 ggaattcagg atcaccagtt ctaaattcta acaatgaggt cataggtgtg gtgtatggcg
     2281 gtattggaaa aattggttct gaatataatg gtgccgtata ctttacgcct caaatcaaag
     2341 attttattca aaagcacatt gaacaataaa caaatttaaa tatacaccat gagcatgtgt
     2401 tcaataattt taatgaaaaa catcggtcga atataacata aaaaaacgtc tatatcaaaa
     2461 gcatcatgaa taaacagagg agcacaaaaa tgaataaaaa tataatcatc aaaagtattg
     2521 cggcattgac gattttaaca tcaataactg gtgtcggcac aacagtggtt gatggtattc
     2581 aacaaacagc caaagcagaa aatagtgtga aattaattac caacacgaat gttgcaccat
     2641 acagtggtgt tacatggatg ggcgctggaa caggatttgt agttgggaat catacaatca
     2701 ttaccaataa acatgttact tatcacatga aagtcggtga tgaaatcaaa gcacatccta
     2761 atggttttta taataacggt ggtggacttt ataaagttac taagattgta gattatcctg
     2821 gtaaagaaga tattgcggtc gtacaagttg aagaaaaatc aacgcaacca aaaggtagaa
     2881 aattcaaaga tttcactagc aaatttaata tagcatcaga agctaaagaa aatgaaccta
     2941 tatcagtcat tggttatcca aatcctaatg gaaataaact acaaatgtat gaatcaactg
     3001 gtaaagtact atcagtgaat ggaaatatag tgacatctga tgcggttgtc caacctggca
     3061 gctctggttc acctatatta aatagtaagc gagaagcaat tggtgttatg tatgctagtg
     3121 ataaaccaac aggtgaaagt acaaggtcat ttgctgttta tttctctcct gaaattaaga
     3181 aatttattgc agataattta gataaataaa tcatccatcc atacattgat aaatgatttt
     3241 tagaaattaa caacaaaatc aacaatttta aacatctctg tgattctatt tattcgaaat
     3301 gatttaaaaa ataaaacttc aaaaacctaa ccttatattt atacgaatac ttagaggagc
     3361 acaaaaatga ataaaaatat aatcatcaaa agtattgcag cattgacgat tttaacatca
     3421 gtgactggcg tcggcacaac agtggttgag ggtattcaac aaacggctaa agctgaacat
     3481 aatgtgaaac taatcaaaaa tactaatgta gcaccataca atggtgtcgt ttcgatagga
     3541 tctggaacag gtttcattgt cggtaaaaat acaattgtta ccaacaagca tgtcgttgca
     3601 ggtatggaaa ttggtgcaca tattatagcg catcccaatg gtgaatataa taatggcgga
     3661 ttttataaag ttaaaaaaat tgtccgttat tcaggtcaag aagatattgc cattctacat
     3721 gtggaagata aagctgttca tccaaaaaac aggaatttta aagattacac aggcatttta
     3781 aaaatagcat cagaagctaa agaaaatgaa cgcatttcaa ttgttggcta tccagaacca
     3841 tatataaata aatttcaaat gtatgagtca acaggaaaag tgctgtcagt taaaggcaac
     3901 atgattatta ctgatgcttt cgtagaacca ggcaactcag gttcagctgt atttaacagt
     3961 aaatacgaag ttgtaggtgt tcactttggt ggaaacggcc ctggaaataa aagtacaaaa
     4021 ggatatggtg tttatttctc tcctgaaatt aagaaattca ttgcagataa cacagataaa
     4081 taaatcctta catagataaa tgattttaaa aattaacaac aaactcaaca attcaaatca
     4141 tctctgtgat tccatttatt cgaaatgatt aaaaaaaata aaacttcaaa aagctaacat
     4201 tataattata caaatactta gaggagcaga aaaatgaata aaaatataat catcaaaagt
     4261 attgcagcat tgacgatttt aacatcaata actggtgtcg gcacaacaat ggttgaaggt
     4321 attcaacaaa cagccaaagc cgaaaatact gttaaacaaa ttacaaatac aaatgttgca
     4381 ccatacagtg gtgttacatg gatgggcgct ggaacaggat ttgtagttgg aaatcataca
     4441 atcattacca ataaacatgt tacctatcac atgaaagtcg gtgatgaaat caaagcacat
     4501 cctaatggtt tttataataa cggtggtgga ctttataaag ttactaagat tgtagattat
     4561 cctggtaaag aagatattgc ggttgtacaa gttgaagaaa aatcaacaca accaaaaggt
     4621 agaaaattca aagatttcac tagtaaattt aatatagcat cagaagctaa agaaaatgaa
     4681 cctatatcag tcattggtta tccaaatcct aatggaaata aactacaaat gtatgaatca
     4741 actggtaaag tattatcagt gaatgggaat atagtgtcat cggatgcaat tattcagcct
     4801 ggtagctctg gttcacctat attaaatagt aaacacgaag ctattggtgt aatctatgcc
     4861 ggtaataagc catcaggtga aagcacaaga ggatttgctg tttatttctc tcctgaaatt
     4921 aagaaattca ttgcagataa tttagataaa taattaaaac ttagacattc acccaatcct
     4981 gacaaaatat actataacta acatttatta atatatattg cattatttaa tatgcatcaa
     5041 agccaatcaa cgattgattt tcaccaactc aattgttgat tggttttatt tatgtatgaa
     5101 tgaacaactt tttgacatca ttaagaatat aaatgatttt gaaagcattt gaaagctaca
     5161 acatttctat aaaatttttc aataacaatt gcgccactaa aactcaaaat ttccaccacc
     5221 aacatccaaa ttatcaacat cgcaacataa ccaaatgtta taataaatct attacacaaa
     5281 gagataaatt acttatgcaa aggcggagga atcacatgtc tattactgaa aaacaacgtc
     5341 agcaacaagc tgaattacat aaaaaattat ggtcgattgc gaatgattta agagggaaca
     5401 tggatgcgag tgaattccgt aattacattt taggcttgat tttctatcgc ttcttatctg
     5461 aaaaagccga acaagaatat gcagatgcct tgtcaggtga agacatcacg tatcaagaag
     5521 catgggcaga tgaagaatat cgtgaagact taaaagcaga attaattgat caagtcggtt
     5581 acttcattga gccacaagat ttattcagtg cgatgattcg tgaaattgaa acgcaagatt
     5641 tcgatataga acatctagcg acggcgattc gcaaagttga aacatctaca ttaggtgaag
     5701 aaagtgaaaa tgactttatc gggctgttca gcgatatgga tttgagttca acgcgactag
     5761 gtaacaatgt caaagaacgt actgctttaa tctctaaagt catggttaat cttgacgact
     5821 taccattcgt tcacagtgac atggaaattg atatgttagg tgatgcatat gaattcctaa
     5881 t
//
LOCUS       DALAAR010000116         5862 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000116 DALAAR010000000
VERSION     DALAAR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.121"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..376
                     /gene="tnpA"
                     /locus_tag="O2458_002574"
     CDS             <1..376
                     /gene="tnpA"
                     /locus_tag="O2458_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCZ3888413.1"
                     /translation="IGIILRQLCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGY
                     LKGKSSLMIFDRHANLKYRYGNRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQ
                     ISMEEYLDPFTGEKNKKRKKKE"
     gene            complement(512..2104)
                     /gene="pckA"
                     /locus_tag="O2458_002575"
     CDS             complement(512..2104)
                     /gene="pckA"
                     /locus_tag="O2458_002575"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCZ3888414.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      2259..2369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2475..3668
                     /locus_tag="O2458_002576"
     CDS             2475..3668
                     /locus_tag="O2458_002576"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCZ3888415.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            3793..4701
                     /locus_tag="O2458_002577"
     CDS             3793..4701
                     /locus_tag="O2458_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCZ3888416.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            4913..5746
                     /locus_tag="O2458_002578"
     CDS             4913..5746
                     /locus_tag="O2458_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ3888417.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         2008 a          879 c         1108 g         1867 t
ORIGIN      
        1 tatagggatt atattgcgtc aattatgtga aagaaaaggt gtagagataa ttgaagcaga
       61 ggcatgtaaa gatcatatcc atatgttagt tagtatacca cccaaacttg gggtatcatc
      121 atttgttggc tatttaaaag gaaaaagtag tttaatgata tttgatagac atgctaactt
      181 aaagtataga tatggaaata gaaagttttg gtgtaaagga ttttatgtgg atacagtagg
      241 tagaaataaa aaagtgattg aaaattatat tcgtaatcaa ttacaagagg atatcgttgc
      301 agaccaaatc tcaatggaag aatatttaga tcctttcact ggagagaaaa ataaaaaaag
      361 aaagaaaaaa gagtaaccct ttagggttgc tggaatagta gtgcagttgg ctgacttgtc
      421 agtgcccttt tagggctggc cagtaaggaa ggcttatagc cgcagaacaa accacccgtt
      481 cacacgggtg gttttgattt agtattcaaa tttatttgtt gaagctacct ttttcagcaa
      541 tatgttcaac tttttcacca aatttttcga agttcttttc aaaacgttga attaaatctt
      601 ctgcttgtgc tttatatttc tctttgtcgc tccaagcatt aattggattt aaaattgttt
      661 tcggtacatc ttcaatttct acaggaatgc ttaaaccaaa cgtactatct tttgtatatt
      721 ctgcattttt caatttgcca gaaatcgctt ggtttaccat ttgacgtgtg taatgtaagc
      781 tgattctacg tcctacacca tattttccgc cagtccatcc agtattaaca agataaacat
      841 caacatcatg taaatcgata agttcaccta atagatcagc gtaaacagta gggtgtaacg
      901 ggaagaacgg tgctccgaaa catgttgaga atgatggttc aggttctgtc acaccacgct
      961 ctgtaccagc taatttagaa gtgaaaccac tcaagaaatg atacattgct tggtctttat
     1021 ttaactttga aatcggtgga ataacaccaa atgcatccgc agttaagaaa ataattgtat
     1081 ttggatgtgc tgctttagat ggtactacaa tattgtcaat gtgattaatt ggataagcgg
     1141 cacgcgtgtt ttctgtataa cgattgtctt caaagtccac tgaaccatct tctgcaacta
     1201 cagtgttctc taaaattgca ccatatttga ttgcgtcaaa aatctgtggt tctttttctt
     1261 tggaaagatt aattgctttt gcatagcagc caccttcgat attaaagacc ccgtttttat
     1321 tccagccgtg ttcatcatca ccgattagtt tacggtgtgg gtcagccgat aaggttgttt
     1381 taccagtgcc agatagacca aagaataatg caacatcgcc tttttcacca acgtttgctg
     1441 agcaatgcat gctcataata tcttgcatcg gtaagagata attcattaca gagaagatac
     1501 cttttttcat ttcaccagcg tattcagtac cgccgattaa aatgactttg tgtttaaatg
     1561 aaataatgac aaaggtttca gatttagtac catcaacttc tggatctgct ttaaaatgtg
     1621 gtgcagaaac gatagtgaag ttaggtttaa tctttgtagc ttcttctttt gattcaggtc
     1681 taataaacat atttttagca aataaattat gccatgctaa ttcattgatg actgtaagtt
     1741 ttaacattgt atctttatcg ctaccagcgt agcctttaaa tacgtatagt tcatcttttt
     1801 tatctaaata gtctagtact ttatggtata acttcaagaa agtttcttca tcgataggtt
     1861 gattaatttc tccccaatca atgttatctc tatatgaagg ttcagagaca aaaaatttgt
     1921 ctttaggcga acgaccagta tattttccag tacttgcatt aacagcacca agttctgtta
     1981 ataccccttc gttattgtct aagattttat tataaagttg tgtcgtcgaa agttgaaaat
     2041 gtgacgttgg ttttttcagt aatttgtcaa ttttagttgt ttcagtgtat gtgtctactg
     2101 acatcctaaa tccctccaaa gcgataattt aaatgtaagc ctttacattt aattagtata
     2161 acacattaac ataattaatc tacaatattt taccgtaaat ttatttgtga aaaattgaca
     2221 ttaattcatt aattaggtac tattattttt aacggattct cttatcctga gtggtggagg
     2281 gacatggacc caatgaaacc cagcaacctc ttttttataa aagaaaggtg ccaaaccgtt
     2341 tgcagacaaa taggtctgaa cgataagagc gaatggacgt attaggcctt ctctctatat
     2401 taatagttag aaggtctttt ttatttagct cacagagaga gaattttcgt aatataaatt
     2461 taaaggagca aactatgtta aataacaaac gattatttac ttcagagtct gttacagaag
     2521 gacacccaga taaaatcgct gaccaagtgt cagatgcaat attagatgct attttaaaag
     2581 acgatccaaa tgcacgtgta gcttgtgaaa caactgttac aacaggtatg gcattgatcg
     2641 ccggcgaaat ttctacaaca acatatgttg atattccgaa agttgttaga gaaacaatta
     2701 aagaaattgg atacacaaga gcaaaatatg gttatgatta tgaaacaatg gcaattttaa
     2761 ctgcgattga tgaacaatca cctgacattg cacaaggcgt ggataaagca ttagagtatc
     2821 gtgataaaga tagcgaagaa gaaattgaag caacaggtgc aggtgaccaa ggtttaatgt
     2881 ttggttacgc aacgaatgaa acagagacgt atatgcctct agcaatttat ttatcacatc
     2941 aattggcaaa acgtttatca gatgtgcgta aagatggtac gttaaattat ttacgaccag
     3001 acggtaaagt tcaggtcact gttgaatatg atgaaaatga taacccagta cgtattgata
     3061 cgattgtcgt atcaacacaa catgctgagg atgtgacact tgaacaaatt caagaagaca
     3121 ttaaagcaca tgtcatttat ccaactgttc cagaaaactt gattaatgaa caaactaaat
     3181 tctacatcaa tcctacagga cgctttgtaa tcggtggacc tcaaggtgat gcaggattaa
     3241 caggacgtaa aatcattgtt gatacgtacg gtggctatgc acgtcatggt ggcggatgct
     3301 tcagtggtaa ggatcctaca aaagtagacc gttcagctgc atacgctgca cgttatgtgg
     3361 ctaaaaatat tgttgcagca ggtttagcag atcaatgtga agtacaattg gcatatgcga
     3421 ttggtgttgc agaaccagtg tcaattgcaa ttgatacatt tggaacaggt aaagtttctg
     3481 aagggcaact tgtcgaagca gttagaaaac actttgacct aagacctgca ggtattatta
     3541 aaatgcttga tttgaaacaa ccgatttaca aacaaactgc tgcttatggt cattttggac
     3601 gtacagatgt cttattccca tgggaaaaat tagacaaagt ggaagaatta aaagacgcag
     3661 taaaataata agatgaattg aatatagcta aataaaagat tttcggtata taaatgataa
     3721 tatgtatcga aaatctttag cttttctatt ataattaaag ttattcatat attaaaaagg
     3781 agcgttgttg tgatggattt atcttcaccg atagtcattg gactcattat tgcagtagtc
     3841 attgcattaa tcttttttgt ccttttttta gttgccttag gtagtaagaa aaaagttaag
     3901 cgacaaacag aagaaaagta tgaacaacaa gaacaaaaca ttaaaaagtc tcatgaagaa
     3961 gctttagaga aagagcgtat tcaaaataaa aagacaatta caaaacaaca agaagattat
     4021 aatcacatgg ttagtacaaa agatcgtgag attgacgcat taaaattgtt ctcgaaaaat
     4081 catagtgaat atgtaacgga catgagatta attggtattc gtgaaagatt agttaaagaa
     4141 aaacgaattc gaccagaaga tatgcacatt atggcaaata tctttttacc aaaggatggc
     4201 tttaataata ttgaacgaat tagtcattta gtattaacaa gaactggtct ttatattatt
     4261 gattctcaat tgttaaaagg tcatgtttat aatggtatta gtggcggtca atttaaagat
     4321 ttaccaccaa tggaacaagt atttgataca ttggatttag ataaatctag accgcaaact
     4381 attgtgatgg atcaaaatga tgacaaacgt tcattatcat ttgtaaatta ttcagatcaa
     4441 attgaggcga ttaaacaatt ggctgaagac ttacaaaaag aattaggtgc caaatataca
     4501 ccaacttcaa ttttatactt taatcttaaa aatgaaggcg atgtaacgat ttcaaattat
     4561 aatcaaaata gtgctgtcaa agtgcttgtc ggtgctgaac aattagacga gttctttaac
     4621 aaatttgtat tccatggtcg tattcaatac aacgtggaag acttacaaca aatgatggat
     4681 aaaattgaat cattcaatta aatgttaggc tgaagtgatg actatatgtt gttacttcgg
     4741 cttttttagt ttgtcataaa ttaaacagaa ttacttttac aatttgatta atatttgaga
     4801 gatattacta gaattttagt ggttgattgc tctaagtgtt ttttctcatg tattcaactc
     4861 agatttgtta tgataacagg gtataataca tcaaaaaggt gaggcgatat ttatggaggt
     4921 taaaacattt tataatggaa acacgatgcc acaaattggt ttagggacat ttcgtgtgga
     4981 aaatgatgaa aattgcatgg aaagtgttaa gtatgccatt gaacaaggat atcgtagtat
     5041 tgataccgca aaagtttatg ggaatgaaga acaagtaggt gctggcattc gtgccggatt
     5101 ggagtcaact ggtattgcta gagaagactt atttattact tcaaaattat attttgagga
     5161 tttcggtcgt gaaaatgtag cagctgctta cgaagctagt ttatctagat taggtttaaa
     5221 atacttagat ttgtatctag tacattggcc aggtacgaac gaagccgtaa tggttgatac
     5281 atggaaaggt atggaagatt tatataaaaa taataatgtt aaaaatatag gtgtcagcaa
     5341 ttttgaacct gaacatttgg aagctttgtt ggcacaagtg tcaattaaac ctgtaattaa
     5401 tcaagttgaa tatcatccat atttaaccca acataaattg aaattatatt tggcagcaca
     5461 acgtatcgtg atggaatctt ggtcaccatt gatgaatgca caaattttaa atgatgagac
     5521 aattaaagac attgctcaag aattaggaaa gtcacctgcc caagttgttt taagatggaa
     5581 tgtgcagcat gctgtggtta caatccctaa atcggtgaca ccaaacagaa tctctgaaaa
     5641 tttccaaata tttgatttcg aattatcaga tgaacaaatg acgcgaattg atggtttaaa
     5701 tcaagataag agaattggac ctgatccaaa aaaatttgaa ggctagatta aaatcgctca
     5761 actgatgaaa aggttagatg aattgtcagg gcttgggaca ttaagttctt aggcaatgta
     5821 aaaaagctga tttctattaa ttatttgata gaaatcagct tt
//
LOCUS       DALAAR010000117         5658 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000117 DALAAR010000000
VERSION     DALAAR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.122"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            64..1554
                     /locus_tag="O2458_002579"
     CDS             64..1554
                     /locus_tag="O2458_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCZ3888418.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2458_002580"
                     /pseudo
     CDS             complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2458_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3301..3438
                     /locus_tag="O2458_002581"
     CDS             3301..3438
                     /locus_tag="O2458_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888419.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3435..3806
                     /locus_tag="O2458_002582"
     CDS             3435..3806
                     /locus_tag="O2458_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ3888420.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            3935..4555
                     /locus_tag="O2458_002583"
     CDS             3935..4555
                     /locus_tag="O2458_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888421.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(5075..5263)
                     /locus_tag="O2458_002584"
                     /pseudo
     CDS             complement(5075..5263)
                     /locus_tag="O2458_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5472..5642)
                     /locus_tag="O2458_002585"
     CDS             complement(5472..5642)
                     /locus_tag="O2458_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888422.1"
                     /translation="MPSRWVKVKTFKSLSTSKLEKKLQNFKSYNSFDIIEIKRHSYLF
                     LNIVEVYYKDKT"
BASE COUNT         2097 a          680 c          955 g         1926 t
ORIGIN      
        1 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
       61 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      121 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      181 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      241 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      301 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      361 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      421 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      481 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      541 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      601 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      661 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      721 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      781 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      841 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
      901 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
      961 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1021 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1081 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1141 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1201 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1261 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1321 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1381 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1441 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1501 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1561 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1621 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1681 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1741 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1801 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1861 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     1921 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     1981 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2041 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2101 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2161 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     2221 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2281 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2341 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2401 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2461 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2521 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2581 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2641 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2701 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2761 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2821 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     2881 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     2941 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3001 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3061 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3121 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3181 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3241 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3301 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3361 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3421 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3481 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3541 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3601 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3661 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3721 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaacca
     3781 aaatagttaa ttataatagc aaataattta atattttata aactatcatt caaccctctt
     3841 aatttattag gaggtttttt gtatttatgc tttcaaatgt gtgatatact ttgtttgttg
     3901 aaatatagag tatctataga tagggtgatt gagtatgaaa ttcactgaag tggaagttat
     3961 cgaacatctt gtaaaggcat ataaagaagc aggaaagcct acttatcctc atgaaaattt
     4021 atatcgagga cgtaatcata gtatttcagg tattggagaa gacttgctgg gtgcttattt
     4081 gattagtaga ttggaaggtg tccaaatatt tattgatcag cctttatcta tgattgataa
     4141 atctttaagt acaagatatc cggatttatt aatttgtgaa gataatgaaa ttaaaaatat
     4201 actagaagtt aaaatggact taggatatca aagaaaagat tttatagatt attgccgaaa
     4261 gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt gtattgtcta gaaagagaga
     4321 agacaaaatt cctatgaata tagctgatga tattaaattt catgttgtga tttacagtga
     4381 aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct atcgttaatg aaacatgtcc
     4441 acatattgaa gtatatgtcc taacaagcgg tcaacaccct aatttagtaa atgttaatct
     4501 tgaaggtatt aatattaata aagatgaatt tgaaatatta gtaaatgcgt tataaaaaaa
     4561 tagagcatcc tccacgttat ggaggtgctc tgttttttat tgaaaagtat caagttaatt
     4621 aatttaatat gcttaataag ttctaccttg acctttttct ctagcttctg ttcgatctct
     4681 tatgtactca gtacatactg gattttctgt taattcatca actgtattag tttaatcaga
     4741 agacgtgtct actttgtaag cttctaagaa cttattatat gatatagcgt ttgagttttg
     4801 ttgttcttct ataaatttct gtagttattt ttcaaaaacc gcatcattaa ctgataagca
     4861 gaagcgtatc acaaataaaa ctaaaaaata gattgtgtat aatataaaag gaagggattt
     4921 atattaaaat tttgaattca aaaattattg aaagggaagc taccttagaa attgaatcta
     4981 tggccactaa ctgctttttt atatcaccac ttattaaaaa atgtaagaat attcctcagt
     5041 gctaagtgaa ggaggttcta gttttattta atattcatat tgataggtaa tggttaataa
     5101 ttgcctgtaa attattgtta aatatcgttg ttatagtatc atcattcata aaatttttat
     5161 agattttatc aagtatttcc tcatcttcaa tagctgtaaa gcgatgtact agtcttttta
     5221 tgagctatat ataattttgc gaagctaaac tgtttgtaat tattcaccaa tcaggttatg
     5281 tagtgtcacg gttcataggt aattctcttt ttaaaatcat ctaatatttt atataattca
     5341 tataagtgta gtattaacgt tttctacttt atctataaaa tatttagaat tacattagaa
     5401 ttttaaatta tacggctgga aattcaatgc tgtaaaaata gacagatttt aaaaaagtag
     5461 taataaaaag tctaagtttt atctttgtaa taaacttcga ctatatttaa aaataaatat
     5521 gaatgtcttt taatttctat aatatcaaag ctattatatg atttgaaatt ttgtaacttt
     5581 ttctctaatt tggaagtaga taatgactta aaggttttaa cttttaccca acgacttggc
     5641 attttatcac tcctatgt
//
LOCUS       DALAAR010000118         5605 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000118 DALAAR010000000
VERSION     DALAAR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.123"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            147..839
                     /locus_tag="O2458_002586"
     CDS             147..839
                     /locus_tag="O2458_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3888423.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            836..2389
                     /locus_tag="O2458_002587"
     CDS             836..2389
                     /locus_tag="O2458_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ3888424.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            2392..3348
                     /locus_tag="O2458_002588"
     CDS             2392..3348
                     /locus_tag="O2458_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888425.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            3348..4115
                     /locus_tag="O2458_002589"
     CDS             3348..4115
                     /locus_tag="O2458_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888426.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            4082..4849
                     /locus_tag="O2458_002590"
     CDS             4082..4849
                     /locus_tag="O2458_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888427.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            4842..5477
                     /locus_tag="O2458_002591"
     CDS             4842..5477
                     /locus_tag="O2458_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ3888428.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         1935 a          904 c         1103 g         1663 t
ORIGIN      
        1 ctaggacata aatcaatgtc tcgcgaccac agcttaattt ttggtgttca ttcatgactt
       61 taaaaaatcc ttattgcata aatgtacata gtgtagtact attcaaaacg taattattac
      121 gatttgaatt aagcgaggag aatgaaatga ctaagactta tgacgtttgg tggcaaaaag
      181 gtcaagaatc agatgatgat atggcacgag accatcaaga agcttgggag agaacaataa
      241 aaatgcttga tacatctgac atcgaaggga aaacgatttt agatgtggga tgtaatcaag
      301 gcggattttt acgacagtta tacgatacaa caccgtttaa aaaaggtgtt ggcatagatt
      361 tagcacgttt atctttggaa aaggcagaga cattaaaagg acaacgtcca cttacatact
      421 atttaacaga taaaccgcaa gaaacgaagc acgtgtttga tacggcagta agtacgtctg
      481 tcttgtactt aatagaagat attccgcaac atgcaaaaga tttaaaagag gtattgaaac
      541 caggcggtgt ttattacgct tcattcgcgg atttaactaa taacccaagt cgtcagttta
      601 tggatgacac gattaatcaa tatggtgcaa caccttctca gaatcactct ctaaaacata
      661 tcgttgatag ctttgtggat gcaggatttg aagttgcagt aatgaaagag catgtacctg
      721 acgtgattga tttaacacat tatagcgatt tttatttatc accgaatgat tatttacaaa
      781 cactatatga agaatcgttt ttaataaaag caagtgtgaa agaaggtact gagaaatgag
      841 gaaatgtgta ttaatgacgg tagcagcaag tgctacgctc ttattggcag gttgtggcaa
      901 tggtcaaaaa gaagataaag atgttacggt atcgctacct actgaagcaa aggcggataa
      961 acttgacgcg caaggctatg atgcagcgat gcccgtttat agtgcagtgt atgatgcatt
     1021 agttaaatat gataaagata agggtattaa agcaggttta gcagataaat ggagcgttga
     1081 tgaatcaggg aaagtttatg aattccattt gaaaaagaat gttaaattct cagatggttc
     1141 agcattagat gctaaggacg tgaaattctc gattgatcgt gcgaaagcga tgaacaaaga
     1201 ttcgactgta gaaacgttaa aaaaattaga taaggtcgtt gttaaaaatg agcacgtggt
     1261 ccaaattaga ttgaaatctc cttcaaatca agtgttaaat gaattaacac aagtgagacc
     1321 gttgcgtatt atgagtccac attcagtaga agatggtaaa gtaaacggta aatttgaaaa
     1381 agcgattgga acaggtgcat ttgttgttga taaaactggt aaagaaaaaa cgacaatgaa
     1441 gccaaataaa tattttgaca acggtcaccc agtcaattat catcttgcat tccaaacgat
     1501 tgaagatggg gactcaagaa attctgcagt acaaagtggt tctgtagata tttctggtgg
     1561 tgctttaggt atgctctcag acgaacaaat caaacaagat aagaaaaata agaacttaac
     1621 gattgaagat agacctagca cagtaagtca ctttatggca tttaacccta aaaatgatgt
     1681 attaaatcaa cgcacaattc gtgaagcgat aagtaagagc atcgatgcga aagacattgc
     1741 gggcaaatct gtaaatggtc tgttccagaa gaacgtacaa tttgtgacta aaaataatca
     1801 acagccacac gattatgata tgaaagcggc tgaaaggtta cttaaatcag aaggatatca
     1861 taaaaacgat gacggcatct ttgaaaagaa tggcaaacct ttatcattta acttagtcat
     1921 tcaaactgca gagttcccaa attggaaaga taaagctgaa aaagtgcaac gtcagcttaa
     1981 acaagccggt attaagttaa atgtgaaaac gttagattca caatcatact atgatacatt
     2041 atggacgaaa aaagactatg atttgatttt ctatagaacg tattcagatg cattaatgcc
     2101 ttacaacttt ataagttcag tgtttaaaaa taatgatggt caaccagggg tgttagctga
     2161 tgatgaaaca ttaacgaaac agctagacga tttcccatca accgtatcaa aagaagacca
     2221 acagtgttca tttgatgaca tatttaaaca ctttaatcaa caatactatg gtgtgccaat
     2281 tgcttatcca aatgagacgt ttgtagtgag tgataaagta aaacaattca aattctctgg
     2341 acttacggat gcaccaattg attataaagc gttgaaagtt aatgaatagc aatgctcaaa
     2401 cgtacaatta aattcatact ttatttaatc gtaagttcgt ttattatctt cattttagtt
     2461 gagaagacat ctggtaatcc agcgattctg tatctacaac gtcatggtta tacgtcgatt
     2521 acgcaagaca atattgaagc ggcacaacat caacttggct taggacaaca tgtgttacta
     2581 agatatatcg attgggttgg acatgcactc acgggcaact taggatacgg ctttagtacg
     2641 aacgaagcag ttaccgctat gataatggaa gccatcgtgc cgacgcttgt gctaatcatt
     2701 gtctctagtt gtatcatgtt gccatttggc tatattgttg gttacttcgt tgggacgcgt
     2761 ccgcatacac gttacgctaa tggaattcgt ggattcgccc aagtgatgac ctcaatgcca
     2821 gaatactggt tagctatttt attcatttat tatttaggcg tacgttggca attgttacca
     2881 tttgtaggta gtgattcatg gcaacacttt gtgctgccaa tcttcacaat tgttgttata
     2941 gaagggtgtc atatcttatt gatgacagca catctgatta cacaaacgtt agatcaagat
     3001 gcgtatcaac tggcgcagtt aagacatttt tcgttaaaag cgcgtatcat cgtacaaatt
     3061 aaagagatat ttgcaccact aatgacgatt tcaattaaca gtatcattca tttaattgga
     3121 aaagccgtaa tactagaagt catcttcagc atgtctggta taggtaaatt gttgattaat
     3181 gctattaacc aacgagatta tccactgatt cagggcattg tcatctttat cattgtcttt
     3241 attatgctaa tgaattattt aggcgatgtg attattttga agaatgaacc tagacttcga
     3301 cgacgtcata cccagcagtc aggcaatgag aaaagaggta cgatgtgatg aaaaaatatc
     3361 aaacgtacat cgcaataggt tcactattga gtttgatggt tgtattaatt acgtatggtt
     3421 taatgcaaga cacgcaacat ttgaacccac ttgagtcacc taatggacaa cattggttgg
     3481 gtaccgatca attaggcaga gacttcttag taagactgat tgtcggtagt cttgtcacat
     3541 tgagtttaac aggcatagtg attctattaa gcgtttgtat gggacttatc tttggcttaa
     3601 ttgcaggcat agaaagacga tggttagatc aaatcatcat gtttgttgcc gatatgttgc
     3661 tggctattcc gtcatttatt atcgcattag tcatcttaag tttagtaagt aactccatga
     3721 taggtttgat acttgcttta acgattggat ggataggacg ttatttacgt tacttcagaa
     3781 atttaacgcg agatattcaa aaacgtccat ttgttcaata tgcacgattg agtgggaact
     3841 caacattcaa aacgacagta acacatgtga ttccacattt attaagtagt atattcgctt
     3901 tggtaacggc tgactttggc aaaatgatgc tcagcatatc tggacttgct tttctaggac
     3961 taggtattaa accgccgacg cctgagttag gaacaattct ttttgatggg aaaagttatt
     4021 tcaacggcgc accgtggctc ttcttcttcc ctggtgtatt gttaggaggt ttcgccttat
     4081 tatgtcaaat tatcaacaaa aaaataacgc agtaaatacg gtagtcaacg tcaatcaatt
     4141 atcgatttta gatcaagaga aagtattgtt aaaggatgtt gatttgacag taactaaagg
     4201 tgcatttcat tgcattatag gtgaaagtgg cagtgggaaa tcactgttaa caagaacaat
     4261 acttggaatg aaacaatcac aattatgtta tcaaggagat attgacatcg atttaactca
     4321 aacagatgca gtgtttcaag atgttcaaag taatatgttt caaaatataa cattagctaa
     4381 gcatttccaa tacatttatg aagccaatcg cacacatctc actaaacagc gtattaagga
     4441 agatgtctta cagatgatgc aattacttgg tttaagacaa ggggaacaat tgcttgagcg
     4501 ttatcccttc gaacttagtg gaggtatggc acaacgtgtc gcctttataa tgtcattaat
     4561 tagacgtccg aactacttat ttttagatga accaacgagt gcacttgatc aagaaaatat
     4621 taaaaagttt atgcattacc ttcttagggc acaggagcgc taccaaatga ccattgtttt
     4681 tatcacacat gatattaact tagtgaaaga ttgtgccaca catattagta ttatgcagca
     4741 aggtaaattg atagaaaatg gtgaggccgc gtcgatctta actaagccga cacataatta
     4801 cacgaaaaaa ttaattacga ttgcacatcg gagacaacct tatgcttaaa atagagagat
     4861 taaccaaata tatagacacg caactgatat ttaaagagat atcatgtaca attaacgacc
     4921 agcacttact cataagtggg gagagtggtt gtggtaaatc cacattagcc aagattatcg
     4981 ctggcttaga tacggattat cagggcgaat tatatcttaa tgggcgctta cgtgaatctt
     5041 atacgtctaa agagtggatg aagcacatcc aatatgtacc tcaatatcaa cgtgatactt
     5101 taaatcagcg taaaacggta ttagctacat tattagaacc acttaagaat tataaggtaa
     5161 ataaacagcg ttatacatca agcattgaag cagtgcttga tcagtgtaat ttaccacacg
     5221 atatacttaa tcataaagtt tcgacattaa gtggtggcca atttcaacgc gtctggatag
     5281 ctaaagcttt aatattagaa ccagagattc tcatattgga tgaagctaca accaacttag
     5341 atgtcattaa tgaagaagct atacttcaaa tgttgatttc cttaaagatg acacaattaa
     5401 tcattatttc acatgataca tacgtcttaa gccaatttga aggaattcag ttacagctaa
     5461 ataaattgaa taattaagat cacaaatctt aatatggtga atatttaatg gtacctaaaa
     5521 aataaaattt aaactacaat gtctaaatcc atatgttgtt tcattagagg atttaaaaat
     5581 gattataaca ctaaaagatt tcaaa
//
LOCUS       DALAAR010000119         5587 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000119 DALAAR010000000
VERSION     DALAAR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.124"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            69..434
                     /locus_tag="O2458_002592"
     CDS             69..434
                     /locus_tag="O2458_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCZ3888429.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            616..1893
                     /locus_tag="O2458_002593"
     CDS             616..1893
                     /locus_tag="O2458_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCZ3888430.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2297..2653)
                     /locus_tag="O2458_002594"
     CDS             complement(2297..2653)
                     /locus_tag="O2458_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ3888431.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(2918..3340)
                     /locus_tag="O2458_002595"
     CDS             complement(2918..3340)
                     /locus_tag="O2458_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCZ3888432.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3411..5516)
                     /gene="rsp"
                     /locus_tag="O2458_002596"
     CDS             complement(3411..5516)
                     /gene="rsp"
                     /locus_tag="O2458_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCZ3888433.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         1888 a          796 c          902 g         2001 t
ORIGIN      
        1 ggtaaaaact ctttattaaa taaattgatt cgttcatact gaatagataa gcaaaaagga
       61 gttacataat gaagaagaaa aaaaacgcga ttaaagttat cattatatta tttatttcat
      121 taattgttgc agtagcattt ttctttggtc tgaaaacata tcaaggccat aagaatattc
      181 aactaattga cagttacttg gaagagaaaa atctaaaaga taaaattaaa tcagaaaaaa
      241 cagagtatag tgctaaaaaa ggtctcttct ataaggaagt tacatttaaa gatgaacctg
      301 gtgtaactta tgttgtgcaa ccaatcagta ctaataaagg attatttgtt gaaggctttg
      361 atacagagac taagaaaagt ttgaaaactg caaagcataa atatttcaat caaaattata
      421 aaccatcaaa atagtgaaac gttaaactgg tgaagcagtt tagtgccttc aattttaatt
      481 atttgaggta aacagttttt gtatggaaat tagtgatttt tgacatctat aaaagataaa
      541 tattttaatt ttttatagat aattatcaat tttctgatat aattgagtga tgtcagatga
      601 aggggagttt gtactatggc tctattcaag agaaaaatta gcctacctat gcaagttgtt
      661 attgcattag tgttaggtgt tgtcgtagga cttttattat atggacaaga aaacgtagca
      721 aattacatta aaccatttgg tgacgtattt ttaaatttaa ttaaaatgat cgttatacca
      781 gttgtatttt gctcactagc gctttctatt tcgaacgttg gggaatcgaa aactgtaggg
      841 cgttatggct ggaaaacaat tttatacttt gaaattatta caacaatcgc aataggttta
      901 gggattatct tcggtaacct atttaaacca ggtgctggat tagacccaac aaaattacct
      961 aaaggtgata tttctaaata tcaatcaact gcacatgcag cagaacaatc tacatatgga
     1021 aatcatttta ttgataccat tgtacatatt attccgacaa acttttttga agctttaaat
     1081 aagggtgaat tattacctat tatcttcttc gcagtattct ttggattagg attagctgct
     1141 gtaggtaaaa aagcagaacc agttaaagaa tttttaagcg gatcgcttga agctgtgttc
     1201 tggatgatta ataaaatttt aaaattagca ccacttggag tgtttgcatt catttgtact
     1261 acaattatta catttggtgc atccgcatta ttaccactat taaaattagt attagttgtt
     1321 gtctttgcaa tggtgttctt tgtattcgct atactaggac tagttgcatg gatgtgtggt
     1381 attaatatca tgaatattat tagaatcttg aaaagtgaat tgcttttagc attttctaca
     1441 tcaagttcgg aagctgtact tcctgtaatg atgaagaaaa tggaaaactt cggttctcca
     1501 aaagaaatta cttcttttgt tataccaatt ggttatacgt ttaacttaga tggatcagca
     1561 ctttatcaat ctattgcagc attattcgtt gcacagatgt atggaatgca cttaacatta
     1621 tcagagcaaa ttgtgttgat gttaacatta atgattacgt ctaaaggtat ggcggcagta
     1681 ccaggaactt caatcgttgt tttattaaca acattaggtg ccatgggctt accggcacaa
     1741 ggtttagcat taattattgg tgttgaccgt atcttagata tggtacgtac atgtgtaaac
     1801 gttattggta atgcattatc aacaatcgtt atagctaaat gggaaaacgt atatgacaaa
     1861 gcaaaaggtc aagaatattt aaaatcaatt taaaaaatac tatctgacat ttaaagcccc
     1921 ttacaacctt tggttgttag gggcttttat gcatgcgcta aagcgcaggc gaacggaagc
     1981 gtagtgacct caggttgtta gggactttta tgcatgcgct aaagcgcagg cgaaccgaag
     2041 cgtagtgacc tcaggttgtt aggggctttt atgcatgcgc taaagcgcag gcgaaccgga
     2101 gcgcagtgac ctcaggttgt taggggcttt tatgcatgcg caaagcgcag gcgaacggca
     2161 agtggagcta ttcaatatga aagtatttca aatataaaaa gcagcagtaa aatgattcca
     2221 aattggaaat caacttaccg ctgcttttac tcgtatatac cttgatggca actaaaattt
     2281 gtaatttaaa ttacaattag tgtttcatgt gcattttgcc catgtcatga attttcatca
     2341 ttaaaccatg tggtacatcg tcatggtcaa cttttttaac tttaacaatt ttatctgaat
     2401 ctgctaattt ataaacaatt acaaaatcac cttttttaac tttaacatct gaatctgaag
     2461 gtaatttaac atttttttca atactttcat ctttttcact aatcattttt tcaatagtag
     2521 tattatcttt cataattcca tagtatgtat ctttctttcc gccagtcatc atcatgatta
     2581 ctaatacaat acatacaatt aacatgatgg cgattaaact aataccaaga ccaaaacctt
     2641 ttttcttttt catggttcat caatctcctt aaatgtagtt aattctgtta tacctaaaag
     2701 tgcaacagtt tgcaaatgca gtatttatat tacataaaat taatttaaaa agacacaccc
     2761 taatcacgtt aaatgtgaat aaagtgtgtc taaaaaacaa tacattgcaa attgaattgt
     2821 aaacatatta aacactttaa ttatagcgag ttattttaag tatttaacat tgttatatct
     2881 agaattcata aaagttattt cctatataca ctttgtgtta taaatcgatt ttgataggtg
     2941 tatctaatga tttatcattc attagcttaa tggattgcgg aactactctt aaaacacata
     3001 actctggatc ttcttttgaa ctgaaaaagc ttttgtcttg tgtttcccat agccaatcaa
     3061 tcacttcttg ttctgtaacg atttcgattg tcgcttccat ctcaacgaaa ctacgattag
     3121 ttgtgtcgtt atagcctaac aaaacatatg ctgctggatt atctttaatt tctttgacct
     3181 tagcagaatg gatattcgtt ttagtatata aagtaagacc atcattataa aagaccatat
     3241 atctactatt aggtttatta ttatatgcag ttgataatac accaactttt gacgttgcta
     3301 acacgttttc aattgcttga attgcttgtg agttactcat tacaaatcat ctccttatga
     3361 tataaatagc ctttaacgta agaaactaaa cacaagagga ctgttagtat ttagcttggt
     3421 ttaaagcaaa tatatttaac ctcgtcgtga tttaacttga gttcaaatgt acgaccagtt
     3481 tcttcttgaa ctgcaagctc cgttttaggg taattcgttt tgtctaattc atctaaaatt
     3541 tctttttcta tataaacaag ttgatcgctt attggaagca aatgttgaat tgaaccatgt
     3601 tcatgattca atgttttaat aatcatcaaa gagtcttgag gtaattcatt atggaaaatg
     3661 aaatcttgtt ttacgtctga catatatcga tcattaattt tattgaataa taagaaatgg
     3721 tagttattat ctttacgact caatacataa ttatttgtaa tttcaatatc ttcattaaca
     3781 aaaggtctaa acattttata taaatgcatt aatggtaggg ggctaccata tttgttgtac
     3841 aaggaaatat aatttgaatg gtcatctaat aattgatagc taatgccacc gccatatcgt
     3901 aataatgcga ttaagtgata cactatgtca gataattgaa ttggattctt accatcatta
     3961 gtatatttaa atccgcttac agacaggtta ttatatacaa attgtgttga tgatatacct
     4021 gaatctaaaa taaatttctt atactgttca aaatgagtgt cagtttgatg atagtgttgg
     4081 tttttagtga taagatgtgt tgcagttttt gaattttcaa tatcaataaa gtaataatgg
     4141 aatttaagac gctgcatgac atcataaact tgttccacgg ttgaataagt ttctaaaaag
     4201 ccatcgactg tcacactata tttaatttct ttatttttgt ttttaatttt aagatgacat
     4261 aaatgaattt cttctgctgt catagaatta gagcaaaata cgagcataaa tttaactaat
     4321 ttcttgcttg tcttatagtc ttgattgcct tgtaaaaatg ttagtattaa ttgcttcatc
     4381 gtttcgaatt gttgtgttga tttaatagcg attgctaaac ctatattttt ttcgaaaagc
     4441 ttttcaaaac atctatttaa caggttgtaa ttcgtttggc taatctcaat atcgtgaata
     4501 tcgtcaatga aaacgacagg ttttggtaag tgctcaaagt taatatcgta atattcattg
     4561 aaaataaatt gaaatagttc attgaaatta ctaaatcgaa caaatacttt ggcacgctga
     4621 cttcgatctt tcggattaaa atcatctata tgaatatctg ttgtatttgt agccagttga
     4681 gctgttaaat caattgttga aattaactca gtgaattgtg atacgttatc ttgttggaag
     4741 tgtatttgtg gtggttcagt aatcttggaa acgagtgaac ggaactgttt aggactaaaa
     4801 tgcaagtaat tcttaaattg atttgcaaaa tttgtatgac tgttaaaacc ggccaattca
     4861 gaaacagttg taatagaatg ttttgtagaa agtaataaat tgatggcgtt aacaagttta
     4921 atacttgtaa aataatcttt aaaattcata ctcaaatatc gaacaaaaag attagaacaa
     4981 tatgattcag agatgttgca atgcatcgct atatctttta aagacaagtg cgcatcaata
     5041 ttgtcatgaa tatagtttac acagtctatg aacaccggat tgcttaaagc aatatttggt
     5101 aaatattcat gatctattct tataaaagct tctttaagta aagtgtctat aatactttga
     5161 cctatatttt tccccattaa agaagaactt gttggtgtac tgattaaatc ggcaattaat
     5221 gacttaatat aattacttga ttgtaataaa tgacgatcta aataaccatt aaagaaatca
     5281 tcatcttgtt gataaaaata aaaaacgggt atcattaatt cgattatatt ttcgccgtgg
     5341 ttaatattat aaatatcacc atggttaatt aagataatat aattacttac gtctttggtt
     5401 tcaccattaa tcgtaatatt gagtttactt gtaagtgaaa atagcaccat aatattatta
     5461 atattacgac ttggtattgt cgataatgta tgatgtattt taagttggca tgtcatattt
     5521 ctctctcctg cctaatatat attgaaatct atcatttaaa tgtaaaacat ggaaattttt
     5581 aaaatca
//
LOCUS       DALAAR010000120         5387 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000120 DALAAR010000000
VERSION     DALAAR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.125"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            175..531
                     /gene="sarT"
                     /locus_tag="O2458_002597"
     CDS             175..531
                     /gene="sarT"
                     /locus_tag="O2458_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCZ3888434.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
     gene            837..3799
                     /locus_tag="O2458_002598"
                     /pseudo
     CDS             837..3799
                     /locus_tag="O2458_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
     gene            4183..5376
                     /locus_tag="O2458_002599"
     CDS             4183..5376
                     /locus_tag="O2458_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3888435.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
BASE COUNT         2124 a          709 c         1013 g         1541 t
ORIGIN      
        1 atgacaaatt catttattat ccaaaaataa aaacaattga acaaaatcaa aatatagtgt
       61 tatgattcat ttgtgtttat agctagcacc acaaactaaa tatatttgga gttattacta
      121 gttaaagaca aatattattt gacgtgacaa ttaaaagtaa gggatgaaga cttgatgaat
      181 gatttgaaaa gcaagagcaa tattaaatta atgaaaagag tattaactac atatgagctg
      241 cgtaaatatt taaaaaaata tttttgtctt acattagata attatttagt attagcatat
      301 ttagatgtat ttaaaaatga tgaaggtaaa tattttatga gagacattat aagttatatc
      361 ggtatagacc aatctagaat tgttaaaagc gtaaaagatt tatcaaaaaa gggttacttg
      421 aataaatgta gagacccaca tgatagtaga aatgtaatca ttgttgtttc tgtaaaacaa
      481 cacaattata ttaaaaatct tttgagtgaa ataaatatta atgaaacata aagtatgttt
      541 cgagatttta atatcttggt gcagttaaat gtatttttta aatgcgttaa ggtatttttt
      601 attttagaaa ttcaatgcta ttgagttgat gggtttttcc aacagataag aatgaattat
      661 tcattgaaaa ctaaaaataa taattttatt tgtagattgt tttaagtgcg tcaatatata
      721 aaataatttt ataaagataa gtgttatatt ccattgtgca actcctattg tatttgtaaa
      781 attacatgta aattcactgt aagcaaagtg gaaaatatgg aaaaaggagt atgcaaatga
      841 gagataagaa aggaccggta aataaaagag tagattttct atcaaataaa ttgaataaat
      901 attcaataag aaaatttaca gttggaacag catctatttt aattggctca ctaatgtatt
      961 tgggaactca acaagaggca gaagcagctg aaaacaatat tgagaatcca actacattaa
     1021 aagataatgt ccaatcaaaa gaagtgaaga ttgaagaagt aacaaacaaa gacactgcac
     1081 cacagggtgt agaagctaaa tctgaagtaa cttcaaacaa agacacaatc gaacatgaac
     1141 catcagtaaa agctgaagat atatcaaaaa aggaggatac accaaaagaa gtagctgatg
     1201 ttgctgaagt tcagccgaaa tcgtcagtca ctcataacgc agagacacct aaggttagaa
     1261 aagctcgttc tgttgatgaa ggctcttttg atattacaag agattctaaa aatgtagttg
     1321 aatctacccc aattacaatt caaggtaaag aacattttga aggttacgga agtgttgata
     1381 tacaaaaaaa accaacagat ttaggggtat cagaggtaac caggtttaat gttggtaatg
     1441 aaagtaatgg tttgatagga gctttacaat taaaaaataa aatagatttt agtaaggatt
     1501 tcaattttaa agttagagtg gcaaataacc atcaatcaaa taccacaggt gctgatggtt
     1561 gggggttctt atttagtaaa ggaaatgcag aagaatattt aactaatggt ggaatccttg
     1621 gggataaagg tctggtaaat tcaggcggat ttaaaattga tactggatac atttatacaa
     1681 gttccatgga caaaactgaa aagcaagctg gacaaggtta tagaggatac ggagcttttg
     1741 tgaaaaatga cagttctggt aattcacaaa tggttggaga aaatattgat aaatcaaaaa
     1801 ctaatttttt aaactatgcg gacaattcaa ctaatacatc agatggaaag tttcatgggc
     1861 aacgtttaaa tgatgtcatc ttaacttatg ttgcttcaac tggtaaaatg agagcagaat
     1921 atgctggtaa aacttgggag acttcaataa cagatttagg tttatctaaa aatcaggcat
     1981 ataatttctt aattacatct agtcaaagat ggggccttaa tcaagggata aatgcaaatg
     2041 gctggatgag aactgacttg aaaggttcag agtttacttt tacaccagaa gcgccaaaaa
     2101 caataacaga attagaaaaa aagttgaaga gattccattc aagaaagaac gtaaatttaa
     2161 tccggattta gcaccaggga cagaaaaagt aacaagagaa ggacaaaaag gtgagaagac
     2221 aataacgaca ccaacactaa aaaatccatt aactggagta attattagta aaggtgaacc
     2281 aaaagaagag attacaaaag atccgattaa tgaattaaca gaatacggac ctgaaacaat
     2341 agcgccaggt catcgagacg aatttgatcc gaagttacca acaggagaga aagaggaagt
     2401 tccaggtaaa ccaggaatta agaatccaga aacaggagac gtagttagac cgccggtcga
     2461 tagcgtaaca aaatatggac ctgtaaaagg agactcgatt gtagaaaaag aagagattcc
     2521 attcgagaaa gaacgtaaat ttaatccgga tttagcacca gggacagaaa aagtaacaag
     2581 agaaggacaa aaaggtgaga agacaataac gacgccaaca ctaaaaaatc cattaactgg
     2641 agaaattatt agtaaaggtg aatcgaaaga agaaatcaca aaagatccga ttaatgaatt
     2701 aacagaatac ggaccagaaa cgataacacc aggtcatcga gacgaatttg atccgaagtt
     2761 accaacagga gagaaagagg aagttccagg taaaccagga attaagaatc cagaaacagg
     2821 agatgtagtt agaccaccgg tcgatagcgt aacaaaatat ggacctgtaa aaggagactc
     2881 gattgtagaa aaagaagaaa ttccattcga gaaagaacgt aaatttaatc ctgatttagc
     2941 accagggaca gaaaaagtaa caagagaagg acaaaaaggt gagaagacaa taacgacgcc
     3001 aacactaaaa aatccattaa ctggagaaat tattagtaaa ggtgaatcga aagaagaaat
     3061 cacaaaagat ccagttaatg aattaacaga attcggtggc gagaaaatac cgcaaggtca
     3121 taaagatatc tttgatccaa acttacccac agatcaaacg gaaaaagtac caggtaaacc
     3181 aggaatcaag aatccagaca caggaaaagt gatcgaagag ccagtggatg atgtgattaa
     3241 acacggacca aaaacgggta caccagaaac aaaaacagta gagataccgt ttgaaacaaa
     3301 acgtgagttt aatccaaaat tacaacctgg tgaagagcga gtgaaacaag aaggacaacc
     3361 aggaagtaag acaatcacaa caccaatcac agtgaaccca ttaacaggtg aaaaagttgg
     3421 cgagggtcaa ccaacagaag agatcacaaa acaaccagta gataagattg tagagttcgg
     3481 tggagagaaa ccaaaagatc caaaaggacc tgaaaaccca gagaagccga gcagaccaac
     3541 tcatccaagt ggcccagtaa atcctaacaa tccaggatta tcgaaagaca gagcaaaacc
     3601 aaatggccca gttcattcaa tggataaaaa tgataaagtt aaaaaatcta aaattgctaa
     3661 agaatcagta gctaatcaag agaaaaaacg agcagaatta ccaaaaacag gtttagaaag
     3721 cacgcaaaaa ggtttgatct ttagtagtat aattggaatt gctggattaa tgttattggc
     3781 tcgtagaaga aagaattaaa ataattcata atttaaataa tagttgattt gcattcacta
     3841 tatttagttt gttaaaaaca acctagaata tgatgagaat gatatacacc ccaaaagttg
     3901 gattgaaaga cctaacttta aggggtgtat ttttgttata aatttgattt ttttgataat
     3961 attttcatat ttaatataat cataattagt agaatataac ctctgagtag tcattttggg
     4021 ggttatgtat ggtgtttcaa gtagtatgta tatttcgaaa taattgatat ttaactgact
     4081 aaattatatt agaattaatt gcgctttgga taaagagagt ttagaagaag gtctaaaaaa
     4141 gctggtggat gcattcaatt atttttatga gaggtgataa atatgttgta tttagaagtt
     4201 ttgaaaaata gaaactttac atatttattg attggtaatt tcttacgtcg gagttgtttt
     4261 gtgctgtttt cgttacaaat aatttggttt actgttgaat tgacaaacca atcatcttta
     4321 aaattatcaa tgatggttat gagtcaaaca cttccattta ttatatttgg tattttcggt
     4381 ggcgcatatt ctgataaaca taataaaaag aaaatacttt atctatctga tcttatacta
     4441 agtttgatta taataattat tcctttactt gcaataacat caaatttaaa ttatctgacg
     4501 cttctaacca tatctacagc tatcaccatt attaactgct atacggatcc tgcgtttagg
     4561 gcaattcttc ctgaaattat tgatgaggag catttagcaa caagtaatgc gttaatcgat
     4621 agtttgcaaa gaggctcaaa tattatttta cctgctttaa ttggtgtcat tgtaatacta
     4681 gttggtaatg ttggcgtctt ttttatttgt agtatattgc tatttttagg atttattttt
     4741 aatgcacttt taaaatatac aaataacaac atgattgata gacactcaaa agaagatttt
     4801 tctgaaactt gggaattctt gaaacaatct aaagaaattc catttattat aatcatccaa
     4861 tttgcctgca tattgattaa tactggcctt tggcgtgttg tattaccatt gtttatttcc
     4921 aatatattaa aagagggcgt tggtgtatat ggactagcta catcatgttt aggtatagca
     4981 tctttattaa tgtcattgat tatgggtttg ctgtcagaaa agcgattaat ctttaaattt
     5041 agtattggtg ttttagtttg gggaattggc ttatcgatta ttaatgtgtt tccaagtgtg
     5101 gcgatattat atataggtgc aacattattg ggacttggac aatctataga gggactaaca
     5161 agatcagttg ccattcaaat aaaaacgcca aatcatttga tggggaaagt atttagtatc
     5221 tcatctacat cgaattatgc agcggataca ttgtctttag gactgataag tataattata
     5281 cctttactaa gcttaagtaa tatatttata ttaggaggtg taattatttc aatacttagc
     5341 ttagctgggt taaagtttgt taagaagtct atgtaaacat tatagaa
//
LOCUS       DALAAR010000121         5303 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000121 DALAAR010000000
VERSION     DALAAR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.126"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(387..1304)
                     /locus_tag="O2458_002600"
     CDS             complement(387..1304)
                     /locus_tag="O2458_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCZ3888436.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            1395..1496
                     /locus_tag="O2458_002601"
     CDS             1395..1496
                     /locus_tag="O2458_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888437.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            complement(1574..3079)
                     /locus_tag="O2458_002602"
     CDS             complement(1574..3079)
                     /locus_tag="O2458_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCZ3888438.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3420..3920)
                     /gene="queF"
                     /locus_tag="O2458_002603"
     CDS             complement(3420..3920)
                     /gene="queF"
                     /locus_tag="O2458_002603"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCZ3888439.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(3940..4806)
                     /locus_tag="O2458_002604"
     CDS             complement(3940..4806)
                     /locus_tag="O2458_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ3888440.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
BASE COUNT         1869 a          835 c          822 g         1777 t
ORIGIN      
        1 taactcatgc attcatattt ttaagtacac attagctgtg actaatgata aagaatcgct
       61 acataatcaa tcattagtcg ttctttatca tttccgtccc gctctcaata aatgttagtt
      121 tatcttatta ttataaatcg gatgaatgtg ttaatctatg gcagattaca cgtcatccga
      181 ttttttatag aatttgaaaa agacgcataa accactatga tttaaaatac aacatcaatc
      241 attttagtgg catgcgccaa aattatatgt ctgtttttga aacagggtaa tagcttaaag
      301 ctaataaaaa cgaatataag gtgcgttgaa tcttatgatt acactccaaa cctaatataa
      361 tatcgggtta agatcattcc ggatgcttac aaatcattga cagtaagtaa ctgaatggca
      421 tttggtataa cctcaatatc aataggtgtt tctaatgaaa tttcgccatc aatatcaact
      481 ttcattgctg gatctgttgt aagtgaaatc tttttaccag gtatatgctc aataccttga
      541 gtaatttcat tccaattcat gctatcacgc tttttaaaaa tatcatttaa aatactgaaa
      601 ctttgttcat taaaaatgaa agtgttcagt tcaccatctt gaggagacaa atcagtcaat
      661 ggtatacgac taccaccaat gaatggacca tttgctgtta gtatcatggt cgtttcgcca
      721 gaatatgtct tatcatctat tgataattga taattaaatt gtgttggatt tagcagtgtt
      781 ttgacagttg atccaatata actcaattta ccaaatatat cttttgaacc atcttgtacg
      841 ttttcagcgt tttgaacaat gagacctaag ccaacaaagt tgagtgcata ttgattattt
      901 attttaatta catcgtatgt accaacttgt gcagaaatca tttgttcact agcttgttta
      961 tgattaggtg ctatatttag cgtttttgta aaatcattaa aagtaccgcc tggtaaaatg
     1021 ccaataggga gttgaaggtc atgtgtcata acaccgttta taagttcgtt aaccgtgcca
     1081 tcaccgccaa gaataaataa tatatctaca tcttttgcat agtttttagt tttgatttct
     1141 tggcaatatt taataatgtc accttcgttt tcactcaatt gaatagaaag atgcttacaa
     1201 attgaactta atgctgttgt aacttcccca ataccttgat taatattttt taatccactg
     1261 tgttcatggt aaaagaggac accatgtgta tatttatttt ccatagttta gcctactttc
     1321 taaaaattgg ttcattaaat atatataccc acttttaatt gttaatacca aaaatatgtt
     1381 tttaaataga gaaaatggta ataaatgaaa ttgatttcta tagagtggga cgagaaaata
     1441 tagttatagc tgtctataat gagcatatta agtttttatt tatactgata tcttgaattt
     1501 aattaataga aacctataaa aaaacagtaa gccatttaaa tgacttactg ttttttgaat
     1561 taggccaaca atattaacgt atacctttca tcgctttgat gattaaaggt gagattgcta
     1621 atacaattgt tgtaacaata attgcaacaa cacctaggaa aataaagtaa tttgtttgac
     1681 ctagtggttc tattaactta actaaagtac cattgattgc ttgtgcagaa gcgttagtta
     1741 agtaccaaat actcatcatt tgggcattaa atgctttagg tgctaactta acagcagcac
     1801 tattacccgt tggtgataag catagctcac cgataacaca aataatgtac gataaaataa
     1861 cccagttaac tgaaaagttt gatgaacctg atgcataacc tacaatacca attagtatgt
     1921 atgacgcacc tgctaagaac gtaccaattg caaattttac tggcaggcta ggttgtttag
     1981 ttccaagctt ttgccataaa agtgaaataa ttggagctag taataaaata aataatgggt
     2041 taattgattg gaagatcgct tcaccaaagt ttgttttcca accaaataag tttaatttca
     2101 tatctgaatg ttcaattcca tatatgttta atacattaga cccttgttct tgaatagccc
     2161 agaacaccat tccaagaata aataatggaa taaatgcttt aacacgagaa cgttcagtat
     2221 cagtgacatc tttacttcta ataattaaag tgaagtaaat gattggtaat gcaataccta
     2281 atactaaaac agtattacta actaagttaa atgataatga gttagttaat gcaccaataa
     2341 cgataattaa tacaattgct aaaacaacac ttccgataat aagaccatac tttttctttt
     2401 cagctggtgt caatgggtta gtaggtttca taccaacgct acctaagttt ttgcggttga
     2461 aaagtacata ccatactaaa cctaatgcca taccaactgc tgcaatcaag aatccgccgt
     2521 ggaagttttt aacattaaca aagtgttgca aaataatagg tgataataat gcacccatat
     2581 taactgacat atagaaaata acaaaacctg catccatacg tctatcattt tcaggatata
     2641 aacggccaac gatatttgaa atgtttggct tcattaaacc tgaaccaata atgatgaaga
     2701 acattgatgt gaataagccg attaatgcaa atggtaagct taaacaaata tgtccgataa
     2761 taataaagac tgcacctaat aaagtagcgc ctctagtgcc tgtaattctg tcagcaatcc
     2821 atccgcctgg tattgatgtc atatagatta atgaaccata aactgacata attgacatag
     2881 ctgttgtttt atcaattcca aggccattat ctgttacggc aaagtacatg tagaaaatga
     2941 gtagggcacg catgccataa taactaaacc tttcccagaa ctctacaaag aagagtacgc
     3001 ctagtcctcg aggatgcccg aaaaatcctg tttgaggtat gtcttgaatt tgatttccat
     3061 gggagttttg ttgtgtcatg tatacatccc atcctttctt cccctaatac aatagtttaa
     3121 gtaaatttga ttgtgcctta tcaactaaat attccaacaa atcacaaaaa aataaaaata
     3181 ttgagaatat tctgttatta agcgcgcaca attaattaga gataatataa aaatatactt
     3241 attgacgtta aagtgcaata ggtttctata aaaatgttgt ttaattcgtg tactaatatt
     3301 cttttggtgt actttggtat aaaatgttat tttaatgtaa cctattatgt atgctaaatg
     3361 aaaaacacac caataagtta ggaaaatcaa cttattggtg tgtttattaa atgatacatt
     3421 taacgattat ctattttttc gggatataaa tcatgattca tcaaacgatg ctcagccatt
     3481 ttttcatatt tagaatttgg acgtccatag tttgtataag gatcaataga aattccacca
     3541 cgtggtgtga acttgcccca gacttcaata taatgtgggt ccataagctc tatcaaatca
     3601 ttcataataa tattcataca atcttcgtga aaatcaccgt gatttctgaa actaaataag
     3661 tataatttca aagattttga ttcaaccatt ttaacatttg gaatatatga aatatagata
     3721 gttgcaaaat ctggttgccc agtaattgga cataatgatg taaattctgg acagttgaat
     3781 tttacgaaat agtcacgacc ttgatgctta ttatcaaacg attctaatac atcaggacga
     3841 tagtcaaaat tgtaagtatt gtcttgattt cctaataaag ttatatcttg taattcatct
     3901 tgttgacggc catgtgccat ataaagcgct cctttaaatt tattttttta ttattttggc
     3961 gtctcggcgt gctttttcaa acatgtaata acttgcaccg ataataacga cgtaacctaa
     4021 tgttgcatag aaatctggag attctccgaa tagaataaat ccaagtattg ctgtgaaaat
     4081 tatagatgca tacgtaaaaa tagaaatatc ttttgctgct gcaaaactat atgctaaagt
     4141 aacaccaatt tgacccacag cggcagctaa gccagcccct aatagataaa gtatttgcat
     4201 ctgactcatt ggttcataag tatatgcagt gaaaggtatt aaaacgatga cagaaaataa
     4261 ggagaagtaa aatactatag tatatggtgc ttctcttgta ctaagtgctc gaacacatgt
     4321 atatgctgat gctgcaaaaa tacctgagaa taagccagct aatgatggaa tcatagatga
     4381 tgaaaattca ggtttcacta ttaaaagcat acctaaaata gcaattatca ttgctgtaat
     4441 ttgatacttc cttacctttt catgtaagaa aacaatgctt aataaaatcg tccagaaagg
     4501 attgagtttc attaatgaat cggcatcact aagtaccata tgatcaatgg cataaatatt
     4561 taacaataca ccaataagtc caagtgttga tcgtgttatt aataagggtt gacttgaaag
     4621 tctgccaaac attggctgat ggtatttata tataaaaaat aatggaataa acattgctac
     4681 taagtttcgt gctaatgatt tttgaaaaac aggaaggtca cctgcaagtc tgaaaaacac
     4741 tgacataaaa ctgaaaccaa tagccgaaat taaaatggca atgatacctt ttactttagg
     4801 attcaatttt atcgcctctt ttatataaaa ttaacgtatt tatattagca taaaacaaca
     4861 tgttgtgcat aaatagttga aatttactat aaaaagacta taatagactg tagcgaacaa
     4921 acgttctgtg tttatttgtc ggaataatag ggcattacac ttttatgaat gtttgtgtta
     4981 ttacataaaa caaatatcaa ttcagtatca agctaataag ctttttcttg atttctgttg
     5041 atacaattga gattgacaca gatttaaaaa aatcaagtga tatctactaa aaaatttttt
     5101 taaatttgtt caagtttttc taatttagta ttggtgccta gttggaacgt tttacgaaca
     5161 ttcgattaga aaatggcact ttaaatcata gtgtgtctta tgtataatga aacacataat
     5221 atagtgttgg tgaaacgaaa aagacacaat atcttgtgtt ttgtatgcaa atgctttatt
     5281 tatgaagaaa ttacatttaa aag
//
LOCUS       DALAAR010000122         5137 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000122 DALAAR010000000
VERSION     DALAAR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.127"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            120..446
                     /locus_tag="O2458_002605"
     CDS             120..446
                     /locus_tag="O2458_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCZ3888441.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            448..927
                     /gene="rsbW"
                     /locus_tag="O2458_002606"
     CDS             448..927
                     /gene="rsbW"
                     /locus_tag="O2458_002606"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCZ3888442.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            902..1672
                     /gene="sigB"
                     /locus_tag="O2458_002607"
     CDS             902..1672
                     /gene="sigB"
                     /locus_tag="O2458_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCZ3888443.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2106..4256
                     /locus_tag="O2458_002608"
     CDS             2106..4256
                     /locus_tag="O2458_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCZ3888444.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4249..4704
                     /locus_tag="O2458_002609"
     CDS             4249..4704
                     /locus_tag="O2458_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCZ3888445.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1965 a          635 c          993 g         1544 t
ORIGIN      
        1 atttaaaaaa gaagattaga aattatttcg atgggtatat aataatttga aatataaata
       61 tggtggatac agcgcttaaa atgaagataa atatttttaa taagtaggag tgtaatgaaa
      121 tgaatcttaa tatagaaaca accactcaag ataaatttta cgaagttaaa gtcggtggag
      181 aattagatgt ttatactgtg cctgaattag aagaggtttt aacacctatg agacaagatg
      241 gaactcgtga tatttatgtt aatttagaaa atgtgagtta tatggattcg acaggtttag
      301 gtttattcgt aggtacatta aaagcattaa accaaaatga taaagaacta tacattttag
      361 gtgtgtcaga tcgtatcggt agactatttg aaattactgg tcttaaggat ttaatgcatg
      421 ttaatgaagg aacggaggtc gaataacatg caatctaaag aagattttat cgaaatgcgc
      481 gtgccagcat cggcagagta tgtaagttta attcgtttaa cactttctgg cgttttttcg
      541 agagctggtg ctacatatga tgatattgaa gatgccaaga ttgcagttag tgaagctgtg
      601 acaaatgcag ttaaacatgc atacaaagaa aataacaatg tgggcattat taacatatat
      661 tttgaaattt tagaagataa aattaaaatt gttatttctg ataaaggtga cagttttgat
      721 tatgaaacaa ctaaatcaaa aataggtcct tacgataaag acgaaaatat agacttttta
      781 cgcgaaggtg gcctaggttt atttttaatc gaatctttaa tggatgaagt cacagtatat
      841 aaagaatctg gtgtgacaat cagtatgact aagtatataa aaaaagagca ggtgcgaaat
      901 aatggcgaaa gagtcgaaat cagctaatga aatttcacct gagcaaatta accaatggat
      961 taaagaacac caagaaaata agaatacaga tgcacaggat aagttagtta aacattacca
     1021 aaaactaatt gagtcattgg catataaata ttctaaagga caatcacatc acgaagattt
     1081 agttcaagtt ggtatggttg gtttaatagg tgccataaat agattcgata tgtcctttga
     1141 acggaagttt gaagcctttt tagtacctac tgtaatcggt gaaatcaaaa gatatctacg
     1201 agataaaact tggagtgtac atgttccgag acgtattaaa gaaattgggc caagaatcaa
     1261 aaaagtgagc gatgaactaa ccgctgaatt agagcgttca ccttctatca gtgaaatagc
     1321 tgatcgatta gaagtctcag aagaagaagt gttagaagca atggaaatgg gacaaagtta
     1381 taatgcgtta agtgttgatc attccattga agctgataaa gatggttcaa ctgttacgct
     1441 attagatatt atggggcaac aagatgacca ttatgactta actgaaaaac gtatgatttt
     1501 agaaaaaata ttacctatat tatctgatcg cgaacgagaa atcatacaat gtacgtttat
     1561 tgaaggattg agtcaaaaag agacaggtga gcgtatcggt ttaagtcaaa tgcatgtatc
     1621 acgacttcag agaacggcaa ttaagaaatt acaagaagca gcacatcaat agaatttgtt
     1681 tattaatgat acgttttata atgaaaaatc catataatta tcccttgatt attaaattga
     1741 aatcgagggg tatttttaat ttaattaaga ttttcgaatt aatacattat tagtgtagtt
     1801 taatgtgtat ccacataaat gtcgcgatat agtattaata atttaagtga agaagatatc
     1861 taattgtcgt tttaaatagg tgggttgcta ttagaataaa aaaagtagtc ttagattatg
     1921 aaatttagaa atgatggtgt gtcattttca ataatcttag tgcgttttaa aatatagtat
     1981 gacctaatca ttcgttttaa atgttttgga agtgaaaatt acattaagta tcatacctta
     2041 atagaagtat tttagaatat gttaaaataa atgagtaaat ttaagaaaaa gtgtgggtta
     2101 agtaaatgga caatcaattg attaattcaa tcatagagaa atatcaattt agtaaaaaac
     2161 aaattgaagc agtattaaca ctgctagaag aaaaaaatac agtaccattt attgcgaggt
     2221 atcgaaaaga gcaaactggt ggactagatg aagttcaaat aaagcaaatt gatgacgaat
     2281 accaatatat ggtcaattta caaaaacgta aagaagaagt tatcaaaaat atagaacagc
     2341 aaggattact tactgaggaa ttaaagaagg atattttaaa acagaacaaa ttacaacgtg
     2401 ttgaagacct atataggcct tttaaacaaa agaaaaagac aagggcaact gaggcgaaac
     2461 gtaaagggtt agagccatta gcgatatgga tgaaggcacg taaacatgaa gtctcaattg
     2521 aagaaaaagc acaacaattt ataaatgaag aagtgcaatc ggttgaagat gctatcaaag
     2581 gtgcacaaga tattattgcg gaacaaattt cagataatcc taaatataga acaaaaattt
     2641 taaaagatat gtatcatcaa ggtgtgttaa ctacatctaa aaagaaaaat gctgaagatg
     2701 aaaaaggtat ttttgaaatg tactatgcat atagtgagcc aattaaacgc attgctaatc
     2761 atagagtttt agctgttaat cgtggtgaaa aagagaaagt attatctgta aagtttgaat
     2821 tcgatacgac atcagtagag gatttcattg cacgtcaaga aatcaatcat aataatgtaa
     2881 atcgcagtta tattttagag gcgattaaag atagcttgaa acgcttaatt gtcccttcga
     2941 tagagcgtga aatccatgct gatttaactg aaaaagctga aaatcatgca atagatgttt
     3001 ttagtgaaaa cttaagaaat ctattactgc aacctccaat gaaaggtaaa caaatattag
     3061 gcgtagatcc agcatttaga acaggttgta aattagcagt cattaaccca ttcggtactt
     3121 ttatagcaaa aggtgtgatt tatccgcatc caccagtttc taaaaaagag gcagcagaga
     3181 aggattttgt acaaatggtt aaagcgtatg atgtgcaatt aattgcaatt ggcaatggta
     3241 ctgcaagtcg tgaaacagaa caatttgttg cagatttaat taaaaagcat cagttgccag
     3301 tacaattcat cattgtcaat gaagcgggcg cttcagtata ctcagcatca gaaattgcta
     3361 gagatgaatt tcctgatttt caagtggaag aaagaagtgc cgtatcaatt ggacgacgcg
     3421 ttcaagatcc attaagtgaa ttagttaaaa tcgatcctaa atctattggt gtcggacaat
     3481 accagcatga tgttaatcaa aaagcactag aaaatgcatt aactttcgtt gttgaaacag
     3541 cagttaacca agtaggtgtt gatgtcaata cagcatcatc atcattatta caatatgtgt
     3601 ctggtctaag ttcgcaaatt gctaaaaata taattgcata tagagaagaa aatggcgcaa
     3661 taaagcataa taaagagtta agcaagatta agcgtttggg tgctaaaact tttgagcaga
     3721 gtataggatt tttaagaata gtagacggtt cagagccatt ggataataca tcgattcatc
     3781 ccgaaagcta taaagtaacg tatcagttat tagataaact cggatttggt ggcaatgatt
     3841 taggtagtga tgcattaaaa gctaaattaa attcattgga tatggatgaa ctggcgattg
     3901 aattacaagt cggtgtacct acattagagg atattattaa atccttgaaa gcacctaatc
     3961 gagatccaag ggacgagttt gaaacaccaa tccttaaatc cgatgtgcta tcaattgaag
     4021 atttacaaga aggaatgaaa ttgagtggta cagttagaaa tgtagtagat tttggagcat
     4081 ttgtagatat aggtgttaaa caagatggac ttgtccatgt ttcgaaactt tctaagaagt
     4141 tcgtaaaaaa tccaatggat attgtcagtg ttggtgatat cgttgacgtt tggatttaca
     4201 gtattgacaa aaataaagac aaagtatcat taacgatgat tgatccacat gaataatgac
     4261 aaacttcagc gaatggttga gaatctttcg gaagaaaaat ttggacgtac gtttcgacat
     4321 tgtgcatatt ttaataaaag gttacgtaca acaggcggac gttatctttt aaagtcccat
     4381 gatattgaaa ttaatccaaa acaatatgag cattatggag aagatgctgt tgtaaaaatt
     4441 atactgcacg aattgtgtca ttatcacttg catattgctg gtaaaggtta tcaacataaa
     4501 gatcaagatt ttaaacgatt gagccaacag gttggagcgc ctagattttg taacagcatt
     4561 gaaagctatc aacaacgggc aaattatgaa tattattgca ctaaatgtca tgcaaaatat
     4621 atacgtatcc gtaaagttga tacgaatcgt atgcgttgcg gacattgcaa tggaaaatta
     4681 agaatgaaaa gacaattgaa ataagtacaa tacaaataga gctaattcgt gtatatgtaa
     4741 gattatttat gagttgtatg gaatttatat agaatttatc agtgagttat tttgattggt
     4801 aaacatagta attgaataag aagaaattgt gtttataaaa tgtttaaagc cgagattgtc
     4861 gtaatttaat acttcgcaat ctcggctttt ctacttatga tataggtaat ggcataaatt
     4921 gtggcttgat aggatgaaat taaaatgatt agattaaaaa tcttggcgta ttttagtaat
     4981 aattaatata taaactttac ccaattaaag ctttaaagta gcacaaaaga taattataca
     5041 tttttcatga cattttgatt gaacttttct attgactgta cagcttatag ttagtatgat
     5101 taaacacgtt ccttaaaaaa gaacaaatta aataaac
//
LOCUS       DALAAR010000123         5108 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000123 DALAAR010000000
VERSION     DALAAR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.128"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(425..1171)
                     /locus_tag="O2458_002610"
     CDS             complement(425..1171)
                     /locus_tag="O2458_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCZ3888446.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1176..1649)
                     /gene="ytkD"
                     /locus_tag="O2458_002611"
     CDS             complement(1176..1649)
                     /gene="ytkD"
                     /locus_tag="O2458_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCZ3888447.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1715..1972
                     /gene="yidD"
                     /locus_tag="O2458_002612"
     CDS             1715..1972
                     /gene="yidD"
                     /locus_tag="O2458_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCZ3888448.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1969..2970)
                     /gene="menC"
                     /locus_tag="O2458_002613"
     CDS             complement(1969..2970)
                     /gene="menC"
                     /locus_tag="O2458_002613"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCZ3888449.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2975..4453)
                     /gene="menE"
                     /locus_tag="O2458_002614"
     CDS             complement(2975..4453)
                     /gene="menE"
                     /locus_tag="O2458_002614"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCZ3888450.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4612..5067)
                     /locus_tag="O2458_002615"
     CDS             complement(4612..5067)
                     /locus_tag="O2458_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCZ3888451.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1513 a         1037 c          785 g         1773 t
ORIGIN      
        1 tacttacaat tccattttgt atgtgctaaa ctgtttgtgt cagatgacat taaatagcat
       61 ctcctcgtgt tgattatttt ggttggctga ccaatattta ttctagcacg tagagatgca
      121 ttttttgtga caatggtaga accttttctg aaccatacgc atagcgtatg gttttctttt
      181 tacaattaaa gagccaaccg ttgttatagt ctaacaatgg ttggctcctc ttattttatg
      241 tgctaaaaat ttataggcaa ttttattaca acaatgtaca tttaaggtga ccttcatgcc
      301 aaaatcgcat cactcattta atggaagcag cacgtcttca tataaagtac cgatccctaa
      361 ttcaacgcat gtagtaccac atcttcaaag cttgatagtt cccatgcgca caccacgttt
      421 catactagct atgcgactca acttggttca taaactcttt aatataagtc aatgtttcaa
      481 ccatcgctgg tggtcttggc acatgtcctt ctgccatttg ataaaatgtt tcatgcgtgg
      541 caccttttaa ctctagttgg tccgctaaat aatacgcatg atgaatacca acttgctggt
      601 ctttccctcc atgtacaatt aatattggcg gactgttttc attaatgttt ggaatcgctt
      661 ggcgtgcctc atatgccgct cgatcttttt tcggatgacc aatcattctt cgtagcatgc
      721 ctcttaaatc gacacgttct tcatacatta aatcaatatc tgagacacca ccccagattg
      781 tataacttgt tactggtaag tcttgaaatg tcaacaatcc ttgtaaacca cctcgcgaaa
      841 aaccaaccat gtggataaat gcatgtggat atttatcatg tagcaacctt aataattgcg
      901 tcacatcatt taaatcgcca cggtaaaatt cgtctttgcc ttcactccca ttgttacctc
      961 ggtagtatgg cccaatcact aaagtttgac tatctgaaaa ttgcattaat ctacctgcgc
     1021 gcacacgtcc tacttgacct ttgccacctc gcaaataaac tacaatgcga tttacttcat
     1081 gatgtggtgt catcattaaa gcttttactt gtaagtcatc tgacaaatat gtaatttctt
     1141 cgaattgatg cgtaaaatat tcaattggca ttcgtttacg tttgataaaa cccaagtgat
     1201 tgcaccctct ctacgcattt taaaatggta ctatcttgca gtaagaaact ccgttgtgcg
     1261 agttcaatat cattgataca gttaaacaac actggccctg ctgtttctaa ataatcgttc
     1321 ttgcttacca atgattcaac ttcgataaaa tatacatctt ttacaaaatc agtttgatca
     1381 tgtgtttcaa tggtatattg tgctatgtaa taaatatttt taactttggc gcctgtttct
     1441 tcatataatt cacgtgtaac tgcttcagca ctactttccc cgcgttccct tttaccacca
     1501 ggaaattcaa tcccccgtaa attatgtttg gtaaaaagca attgattttt aaacgttgga
     1561 atagctagca catgattgcc atctgctatc tcattatcct ttttaaatgt caaattaact
     1621 tgacgattat ctttatccct aaacttcacg cgcatcacat ccctacattg tatgttaata
     1681 taatagttaa ttactatcgt tggaggcatt aattatgaaa aagatattct tggcgatgat
     1741 tcatttttat caacgtttca tttcgccact cactccacca acttgtcgtt tttatccaac
     1801 atgttcagag tacactagag aagcgattca ataccacggt gctttcaaag gcctttattt
     1861 aggtatccgt cgtattttaa aatgtcatcc gcttcataaa ggcggctttg accctgttcc
     1921 gttaaaaaaa gacaagtcag caagcaagca ttcacataaa cataaccatt aatatggttg
     1981 taattgagtt atatccacta aaggggggcg aaattcgagt cgcccctctt ttaatatgcc
     2041 tgaatgcgcc accacatctt gttcaaaata ataacctgct ggtgtaacat ctcctggata
     2101 atcaccttta cgagcaagca tcgctgtaaa atagcggctt aaaccatatt cgtacatgcc
     2161 gccaataacc acttttgcac catgactttt caaagtatca attgccgttt gcactttatc
     2221 aatgccacct agacgaaatg gttttaatac aacaactttc acattgtata attctatcaa
     2281 attaattatg tccaacaacg atgttgcctt ttcatcaagg gctattggag gtattgttcc
     2341 atccgctact tcatcaagca tggagatatc tttaaatggc tcttcgatat aaagaacctg
     2401 ttcacgcgct aataactgta actgtgtgaa atcttgacga tccaaggact catttgcatc
     2461 tataaccaat tgaaagtgaa agtctaattc ccgtaacact ctaatttgat gcatgatttg
     2521 aggcgtccat tttaatttaa ttctggtcgg ctttgttgct tttaatgact ctagttgttt
     2581 atttgataag ccgctcgctg tcgctccata tgctactgaa aatgaaggca gtacatgaaa
     2641 catttgatac aatgccatga caatagttgc ccttgcagca ggcgtatttt ccaatgaatc
     2701 tactaatttt agtgctgctt catacgtttc aaatgattta tttctattat cttcgaacca
     2761 ttgctcaatt acatgtttca ctgaggcaat tgtttcatga tcataccaat ctgtttgaaa
     2821 agcgttacat tccccgaaat atgcatttcc tttgtcatca atcaattcga taaacaaaca
     2881 atcacgatgc gttaaagtga ctttcggtgt tacaatttgt gacttaaatg gctcactata
     2941 tttataaaaa tgcaaagctg tcaacttcat caaatcatcc tctatacaac ttatttcttt
     3001 gtaatttacc tgttgatgta taaggtaaag tatcaacctt ttcaaagtgt ttcggtactt
     3061 tatatttcgc taaatgttgt gataaatatg caatcaattg tgcctttgaa atgtcacttt
     3121 cactgacaaa atataattta ggcacttggc cccaagtatc atcaggatgc cctacacata
     3181 ctgcgtcact gatacctgga aattgcttcg ctaccgtttc aatttgatat ggataaatat
     3241 tttcaccgcc actaataatt aaatctttac gtcggtcata aatcatgaca taaccttcat
     3301 gatctatttc agcaatgtca cccgtattaa aataaccatt ttcaaacgta cccgttaaat
     3361 ctgttggata caaatataca ttcatcacat tggcgccttt aatcattaat tctccatgac
     3421 cttctttatt aggattttta atttttacgt caacattggc acttggcatc cctacagtgt
     3481 caggacgtgc atgcaacatt tccggtgttg ctgttaaaaa ttgcgaacat gtctcagtca
     3541 taccaaatga attataaatt ggcaggttat attgtaatgc cgtctctatc atagtggcag
     3601 ataatttagc accgccgagt aatatttttt gtaaattata aggttcatgt aaaccttgtt
     3661 gcataagcca atttaaagtt tgtggcacaa gcgaaatgtg cgtgattcgt tcatttttaa
     3721 tcatcgttaa aatttgttcg gcattgaatt tatcaacaat gcgcacagta aaaccttcaa
     3781 taacagctct taaaagtaca ctgagacccg aaatatgata aatcggcaag acagatagcc
     3841 aattagtgtc acgatcaaat cccaagctct ctttacatcc gattgcactg gcataatgat
     3901 tacgaaacgt ttgtggcacc gctttttgag ggcccgttgt ccctgatgta aacataatcg
     3961 atgcaatgtc atctaaatta aatgatgtat ttaatatgtt ggacggcgac tctttcggca
     4021 ccacagtttc attcgatgtt tcatattgga tacccattgt gttgtccaac aaactgttcg
     4081 ttgtaatatc ccttccagcg aattcaatat catccagcga tacaatttga aaccctcgta
     4141 attccagtgg caaggtacaa aaaatcaatt gtacatcgat tgacttcatc tgattcgtca
     4201 tctcattagg tgtcaacctt gtattaatca tcgcaatttc aatatttgcc aaccaacatg
     4261 catgtattaa aatgatcgat tgaatcgaat tatctatgta tagcccaaca cgagattgtt
     4321 gataagcctt gagtctttta gccaatagac tcgcttcaca gtataaattt tgataagtat
     4381 aagattcttg accgtctgtt atcgcaatat gatgtccatt ttgttgtgct tgtttatata
     4441 accaaaagtc catgcgttat tcctccaaaa tcatttacat tataattata acgattttat
     4501 gacattctag cagtggttat gtttaaaaat ataaaaaagt agacgaattg atgcattgat
     4561 atgattgtta taatgctcaa tacatatcgt tatatcattc gtctactatt atcagttatt
     4621 tttatttaat tttagtgtca ttctgtcatt ttgatgtggt gatttaccca ttgttgccac
     4681 atcatctgca atgtcaattg gtatacggtt catgtcttgt aatgcactta aatggaatac
     4741 ttcatcatct aaattttcaa tgagatatac ataatatgtt accttgtcct ttttatattt
     4801 taacgttttc caaaagtccg gcttgcaatt caatacatta tccggaatat attcaataaa
     4861 taagtaacgt ttgctgccta ctttgtctat gaaatatttt gcagtgcctt tttctatacc
     4921 tcttatatgt gcatagtctg ctgaaaagta aatactacct attgtttcat tatgttgttg
     4981 tatttcaaat cgttggccta ctattttatt atttgtgcta caactactta aaaaaatcag
     5041 tagatatagc attaaacata ttttcatccc cttgaatttt aaaaactttt tcaaaagcaa
     5101 cacctcta
//
LOCUS       DALAAR010000124         5002 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000124 DALAAR010000000
VERSION     DALAAR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.129"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..173
                     /locus_tag="O2458_002616"
     CDS             <1..173
                     /locus_tag="O2458_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888452.1"
                     /translation="HLINGEVIKVQEHMKDAESKTLHIIYPKDRVVSLDHVLYFDINV
                     KGEKNNDSPYPS"
     gene            complement(170..346)
                     /locus_tag="O2458_002617"
     CDS             complement(170..346)
                     /locus_tag="O2458_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888453.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(362..1114)
                     /locus_tag="O2458_002618"
     CDS             complement(362..1114)
                     /locus_tag="O2458_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCZ3888454.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            1165..1494
                     /locus_tag="O2458_002619"
     CDS             1165..1494
                     /locus_tag="O2458_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888455.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(1483..1698)
                     /locus_tag="O2458_002620"
     CDS             complement(1483..1698)
                     /locus_tag="O2458_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCZ3888456.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1714..1977)
                     /locus_tag="O2458_002621"
     CDS             complement(1714..1977)
                     /locus_tag="O2458_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888457.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(1974..2147)
                     /locus_tag="O2458_002622"
     CDS             complement(1974..2147)
                     /locus_tag="O2458_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ3888458.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            2110..2823
                     /locus_tag="O2458_002623"
     CDS             2110..2823
                     /locus_tag="O2458_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888459.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            2839..3771
                     /locus_tag="O2458_002624"
     CDS             2839..3771
                     /locus_tag="O2458_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCZ3888460.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            3777..4118
                     /locus_tag="O2458_002625"
     CDS             3777..4118
                     /locus_tag="O2458_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888461.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            4322..4504
                     /locus_tag="O2458_002626"
     CDS             4322..4504
                     /locus_tag="O2458_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888462.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            4582..>5002
                     /locus_tag="O2458_002627"
     CDS             4582..>5002
                     /locus_tag="O2458_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ3888463.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNT"
BASE COUNT         1769 a          744 c          762 g         1727 t
ORIGIN      
        1 tacatttaat taatggtgaa gttattaaag tacaagaaca tatgaaagat gctgaaagca
       61 aaactctaca catcatttat ccaaaagata gagttgtcag tttagatcat gttttgtatt
      121 tcgacattaa cgttaaaggt gaaaagaata acgatagtcc ttatccatct taaaatccat
      181 agtgcttata gttatttgct atttgttgac agttatcact ggcaagtcct accttgtcag
      241 cttggtaact gtattgatta tttacttcat ttactaatcg attccattcg tctcttggta
      301 tatcttcaag aagtaagaga atcatcttta atttttcttc gttcatttgt tattcctcct
      361 tttaagatgt ttgtttttct cctaaaaact tattaacaaa gtattgttgt cctttgcctg
      421 ttacttttgg cgtcttacta attgatgtgt gaccgtccga atgtgtgatt gatgtttctt
      481 taatttcgaa taactcacgt tccattgaat actgtgtagg catgttataa tccacaccct
      541 tgcgtttaat aaggaatccg ttttgacgta accactcaaa caatctgcgt tgcccgatgt
      601 ttacaccgtt ttgtttaatg atctttgcta actctccaac taaaattgat gtcttagtag
      661 tagctactgc atctgcaaat acaatttttg gtttatcacg ttcaatcttt gtttctaatt
      721 gattgattgt gttgttagca atttttaaag cacgttgcat aatcatttct gggctgttcc
      781 atgctttttc aacttggatg aagtattgtc ttgcacgttt accaggttca ctacgttgaa
      841 tcattgcgat ttcttttgca gtgtctagtg tgagtgcgtg gtcaatatag tgtgtcatat
      901 tgccttgagc tgttgctctt ttttgagcga tagctgtgta atctgtattt tcttcaaatc
      961 cgtatttaag cattcttgga aaccaatctt tatatgctgt cttaacttct aatgcttgat
     1021 gaagttctcg accgctgatt gcgatttctc cattttcttt ttcttgaata ttgaacattt
     1081 ctccgatgtt cgaatttgtt tgtaatgctt gcatattgtt tatgctcctt tctgctatac
     1141 tcctattaag gaggtgaatg acttatgact aatgaagcta aatttgtcct tttacaactt
     1201 tattcaattt accttgatag aattgacgaa ggtatgtcta aacgttctgc atcttatttc
     1261 ggtagtgatg aatcctcatt taacgctttc tttttagatt ttaattttga agactatatc
     1321 gatgcagttc ttgaattaaa gcatagagat tttgtaattg cttctgctga agatggcggt
     1381 tttcttgaga tggctctttc tcgagaaggt atcgcctact cagaatcaga atccaaaaaa
     1441 gattacaaaa cacttatgga tttaattaga gatttgaaaa aattaataat ctaaaatcca
     1501 atcatctgct attaaatcgt ctgcgctagg ctgccacctt ccggcggcgg tttgtctttt
     1561 tttcttatag tgtctagata cgactaggca ttgataacgc tgcaaattag ttggtaatat
     1621 cccatacaca tcttgattct ctctccttat acttattcct ttctccatcg ctagcttcgt
     1681 tgcttcttga atgttcattt gttattcctc ctattaagat gtttgttttt ctttaaatgc
     1741 taaaataatt gatttctttt tatcattcgt aaatacgaaa ttttcgtatt cattacctaa
     1801 aaaaatatca tcatatttaa cattaaaagc actcatatac ttagaaagta aactatcttt
     1861 aatgtttgta gagtcttttt ccatattttg aattgtacgt gatgagacct taaataaatc
     1921 tcctaactct ttttgagtca atccgtaatc agtcctcaac tcttttaatg ttttcatgtt
     1981 gtcaccgcct ttcgtaaacc taatataata cgaaattttc gtattgtcaa cattaaatac
     2041 gtttttttcg taaaaaactt tactatgata tgaaaatttc gtataataag aaaaaaggag
     2101 gtaagtaata tgaacaaaga aagaaatatt attatagcca aaaacattag aaaatttctc
     2161 aacgattcaa atatgtctca aaagaaactt gctgaactca ttaacataaa accatctact
     2221 ttaagcgatt atttaaattt acgttccaac ccctctcacg gcgttataca aaggatagct
     2281 gatgttttcg aggttggtaa aagcgacata gatactacat acaaagacga taacgacatc
     2341 acttccatat acaacaaact cacacctccc cgccaagaaa acgtacttaa ctatgcaaat
     2401 gaacaattgg aagaacagaa ttctaaagga gataacgttg tagatattaa ttcatataaa
     2461 caggagaaaa ctccagttaa cgtcaatggt tgcgtctctg ctggtgtagg agaacgttta
     2521 cacgatgaaa cgctatttac tgaaatggtt aaaggaccta tccccacaca cgatttagcg
     2581 ttaaaagtaa atggtgattc tatggaacct atgtttaaag atggcgaaat catatttgtg
     2641 gagaaaactc acaatataaa gaatggacaa attggtatat tcatcattga agaagaagcg
     2701 tacgttaaga aagtctttgt tgaagatgat agattgactc tagtttcact aaataaagat
     2761 tacgacgatc tacactttta tagaaatgaa agtgtgaggt taattggaaa agttatttta
     2821 taaaaggagc acttgcaaat gaaaaaatat gatattgcag tcttagactt tgaaactatg
     2881 aatgaacata tgaacagccc ttgcgaagtt gctgtatctt taattaagga tttatcaata
     2941 gtaaaagttt attcatctta tattaatcct cctaataata gatataactt gaaaaacgct
     3001 aaaatacata aaatacctga agatgtcata ttaaaagcac ctaaatatcc agatatttac
     3061 caagaaattc tctatctttt aaaagaatca catttaatta ttgctcataa tgcacttttt
     3121 gatatttcag tattaaaaaa tactaataat tattatgact tacctgttcc aaacttcatg
     3181 tatgtcgata gtataaatat ctttagaagc ttccacgcaa tctctagttt taaattagaa
     3241 aatttgtgta gcttatatga tatcgataaa gaaaaattac attctgctaa atttgacgtg
     3301 ctagctttat cgaagatgtt gatatcactc gctaaaaaca atcagcatta tagtgtatta
     3361 aaattaatac attatatgcc taagcaatac attagattta gcaaatattc taactctcca
     3421 actaaacttt tcgattcagg atttcaaaaa attcatatga aaatatctga gattaataaa
     3481 atagaagtgg aaagtgtaat ccctatttta aaagataaaa atgttgtttt tacaggtaat
     3541 tttgacactg aaaaacaaga tttaatgata ttaactagaa agaaaggagc ttatatcaga
     3601 agtgacgtaa ctgcaaaaac agatatttta gtcgaaggtg ttcaagatga taaatataaa
     3661 gatgtgaacg gactagtttc aaaacaacga aaagctcgag aatatgttgg aaatggtgca
     3721 aaaattcaat ttttaaatga agaagactta ataaatttaa taaaggaata ataacgatga
     3781 tcaaaaaaat ttttacaaaa aagcatgtat tcttagttat agaagatgaa aaccataatc
     3841 acagtgatgc tgtttttgga aaaagtatat tactttcaat ttacgtcggt gtgaataaaa
     3901 agactaattc taaatcaggg aaatttatat accttgacag atctaaaaga atcgttagac
     3961 aatctgatat caccaaaata gaatcagcta acgaaaatga tgtagatttt tataatttac
     4021 tgaagaaaga aaaggaaatt gtttattcca aaaatatagt agataaatac aatttagcga
     4081 actatataat ttactacgaa gttagtacta aagaataaac caatccatta tttcataata
     4141 ctaaccttaa atttacagag gttttaatta tgaaacatga aaaaagcaat cttaacttta
     4201 agtcttatat ttattaccta ctacctcact tttaaatata tgtggattaa agaattgaag
     4261 tattaattat gcttatttaa aaaagacgtc tatttcagca gtgtttgaaa ggaagtttat
     4321 aatgaaaata actaattgca aaataaaaag agaaactgta atatacgaag ttttaactag
     4381 tggtaatcaa ccattcactt atgagttacc taaagattta tcgtcacata atgcgcgtaa
     4441 atacttggaa tttatttcac aaaaaataga tggcgataag ttaaccaaag aagattcatt
     4501 atgattttac taatcaaaaa acgtctacaa gtgtagacgt tgaatggtgg tgagagtgtg
     4561 agcgagaata aaggagaaat gatgacgcat aatatagaaa aacgcattaa taaattaaaa
     4621 acttctggaa atccaaaatt taaaaaatta gattcagata ttcactattt actcaagaga
     4681 tttgaaggtg aaaaaaacca taaaggtttt tatccaaagt ttaaacaagg agaaatagtt
     4741 tttgtagatt tcggtataaa cgttaataaa gaattctcta attcacactt tgcaatagtg
     4801 atgaataaaa atgattctaa tacggaagat atagtaaatg ttattccctt atcttctaaa
     4861 gaaaacaaaa agtatttaaa gatgaatttt gatttgaaat gggagtatta tttaagattg
     4921 tttttaaatt taattagcgc gcaaaataat tcagctatat taaaagaagt tttcgataaa
     4981 aaataccaaa aaaacaacac ag
//
LOCUS       DALAAR010000125         4945 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000125 DALAAR010000000
VERSION     DALAAR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.130"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(230..2038)
                     /locus_tag="O2458_002628"
     CDS             complement(230..2038)
                     /locus_tag="O2458_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCZ3888464.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            2155..2547
                     /locus_tag="O2458_002629"
     CDS             2155..2547
                     /locus_tag="O2458_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCZ3888465.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2549..>4945
                     /locus_tag="O2458_002630"
     CDS             2549..>4945
                     /locus_tag="O2458_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCZ3888466.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     G"
BASE COUNT         1821 a          662 c          836 g         1626 t
ORIGIN      
        1 tgtaattcta aacctttttc atcgcgaaaa caattaaata ggtcgcggta ttaattatta
       61 tattattacc gcttaatatg aaaaatacat gaaaattaat tttctaatat acttttgaaa
      121 aattattaca aattagcccc ttcaaaacgc gaaaacataa ggattctagt ttcaaaaggg
      181 ctgataagca taaaatgaaa tgtaatattt cgatgtataa aatttttaat tagctaaaaa
      241 tcatcgcatt aattttttga gctacatcat caaaattcgg acattttaac gacacatata
      301 atttaatttt aggttcagta ccagaaggac gtaaagcgat aaatccttcg tcaaataaga
      361 cacgaataac atttgattta ggagaattaa tctgcgacgt tgtatcttta tccaaatgat
      421 aaacctcgct agttaaataa tcttcaattg ctttcacttt gagtccttga atctcttgcg
      481 gtggatttga acggaatttg gtcattattg cattaatttt ctttttccct tcaaatcctt
      541 ctagcgtatg cgagaataat gtatcctcat gtctaccaac agtttgataa atctgttcta
      601 attcatcttt caatgttttg ccatataatt ttaactcaga agcgtatttt ataatgagtg
      661 gcacaatttg tacggcatct ttatcacgta caaaaggctc tgatagaaaa ccgtaactct
      721 cttcaaatgc gaaaatcata ttttgatgat catccagttg tcttatttcc tgagcaataa
      781 atttaaagcc cgtcagcacc tctttgtatt caacattatt atagcgtgct aatgactttg
      841 ttaactcact acttacaata gattgaatca ttaagcggtg acgtaattga gacgtttgtt
      901 gaatacgata attaagtaac agcgcaccaa tttggttgcc attaaaatat gtgatatgac
      961 cgtgtgcatc acgttcagca ataccaagtc gatctgcatc agggtcagtt gatattaata
     1021 agtctgcatg acttttattt gcaagttcga ctgcttgatc aaaagcgcga tgatcttcag
     1081 gattagcact ttgaactgag ctgaaattcg gatcaggttt acattgtgct tctacaagat
     1141 taaattgatt gaaattcaaa gattgtaata actcaggtac aatcggaaca cttgtaccat
     1201 gcaaactagt gaacacaact tgtaaatcag atttaggaat atagccaatc atattttgaa
     1261 tgtgtttcat atagtcatca gttacagatt tcggaaaagg cttgatataa gatgtatttt
     1321 gtttagaaat aggtatatca atctgtaatg gatcgccaac ttcttcgata taacgacttg
     1381 caagctcaga tgcatcagtc gataattgcg caccatcaga accatatact ttgatgccgt
     1441 tatagtcttt cggattatga cttgctgtaa tcataatgcc agcagtagta ttaagattgc
     1501 gcaccgcgaa cgataattcc ggtgtcgttt tatatgtatc aggtaaataa actgttattt
     1561 gatggtttgc taatacatta gcaattattt gggcgaattc agttgaaaga tgtctaatat
     1621 cataatgaat gactattgtt ggactgtttg tttgggcatt taaataacgc gctaaaccta
     1681 atgccaattt ttcaatagta aacttattaa gtcgaccttc accaagaccg aattttccgc
     1741 gtatacccgc agtaccaaat gttaatttac tttcaaaacc ttctcgctgt tcaatgtcag
     1801 attgctgctc ataaaaatgt ttaactaaac tatcattagc tcgttctatc cataattctt
     1861 tatccattgt tgctaaacat cctttcaaaa tctcagttag acttaataaa acatgaaaac
     1921 taaagccctt acatttatgt aatgaattat aaagaaatac gccccaaaag taaaaaaaca
     1981 cagccccaag acaatacttt tcacaagtat tatataatag atgtgtatga aaatgcatgg
     2041 agtagagcgt tcatgtgaat gtaaaatatt aatttttaat attgatttat atatagtttt
     2101 gtaatttttg atagaataac aattaagatg attttaaatt gaagggtgag tcttatgaaa
     2161 aaagtaatca atgtagtagg agctattatt ttttctgata acaaaattct ttgtgcacag
     2221 agaagtgaaa aaatgagtct gcctttaatg tgggaatttc ctggcggtaa ggttgaaaag
     2281 aatgaaactg aaaaagacgc tttgattaga gaaattagag aagaaatgaa atgtgattta
     2341 attgttggag acaaagttat aactacagaa catgaatatg attttggaat tgttaggtta
     2401 acaacataca aatgtacttt aaacaaagag ttaccaactc taactgaaca taagagtatt
     2461 gaatggttgt caataaacga acttgataaa ttaaattggg ccccagcgga tataccagcc
     2521 gttaataaaa ttatgactga gggataatat gagtagatta ctaaatgatt tcaatcaatc
     2581 tttacataaa gggtttatag acaaacatat ttctcataaa gggaattata caccgaagct
     2641 tttagtaaat aataaaaatg aaaaagttct ttcaacaatt atagatgaac ttcaaaaatg
     2701 tgaaacgttt tatttttctg tggcttttat aacagaaagc ggtttagcca gcttaaaggc
     2761 tcaactatta gatttaagca ataagggtgt taaagggaaa atattaacat ctaattactt
     2821 agggtttaat agtcctaaaa tgtatggaga attacttaaa ttaaaaaatg tagaggtaag
     2881 attaactgat attgctggat tccatgccaa aggatatatt tttgagcata aggattatag
     2941 ttctatggta ataggaagct ctaatttaac atctaatgcg ttaaaggtta attatgagca
     3001 taatgtttta ctgtctacta tgaaaaatgg cgatttagtt gatagtgtaa aaagtgaatt
     3061 tgacttgtta tggcaaaaga gtaccccact gactgagcaa tggattaatt catataaaga
     3121 atcatttgag taccgttcct tagaaaaatt agcggaggtt gaacaaactc aaatgctgct
     3181 agctgataag gtgaaaaaat cagtggaaat tgtacccaat ttaatgcaag cagaagcatt
     3241 aaggtcatta aaagctatta gggataaagc caaagataag gcgttaataa tatctgcaac
     3301 tggtacaggt aaaacgattt tatgtgcatt agatgttaga gaagtaaacc ctaataaatt
     3361 tttatttatt gttcataatg aggggatttt aaatagagct aaggaagaat ttaaaaaagt
     3421 attacctata aaaaatgata gtgattttgg attgttgaca ggaaaacata gagatgtcga
     3481 tgccaaatat ttatttgcaa ccattcaaac attatctaga gatgataatt ttaagcaatt
     3541 tgatgaaaat gaatttgatt atattgtttt tgatgaagca catcgttctg ctgcatctac
     3601 ttatcaacga gtatttaact acttcaaacc taagttcatg ctgggaatga ctgctacacc
     3661 agaaagatca gatgaattaa gtatatttga actgtttgat tataatattg catatgaaat
     3721 aaggttacaa gcagcattag aaagtgatat tttatgtcca tttcattatt ttggtgtgac
     3781 tgattatgta catcaaggta ttaaagaaga tgatgtaact aaactgagat atttaacttc
     3841 tgatgaaaga gttaattata ttattcaaaa gacagattac tatggatatt caggtgaaat
     3901 tttacaagga ttgatttttg taagtagcaa aaaagaagct tatgatttag cggataaact
     3961 aagttcaaaa gggattaaaa gtgttgcttt aacaggagat gatagtgtta attatcgcca
     4021 aattgtaata gaaaaattaa aagaaggtaa gattaactat attataactg tagatctttt
     4081 taacgaaggt attgatattc cagaagtcaa tcaagttgta atgttgagac caacagagtc
     4141 tagtattata ttcattcagc aacttggtag aggattaaga aaaagttcaa ataaagagta
     4201 tgttacagta attgatttta ttggtaatta taagacaaat tatttaattc cgattgcgct
     4261 ttctggggat caatcgcaaa ataaagataa ttataagaag tttttaacga ataacgattc
     4321 gattaatgga gtatctacaa ttaattttga agaagttgct aaaaaacaga tttacaattc
     4381 attggatgca gtatcattaa atcaaaataa attaatatta aaagcttatg aagaagttga
     4441 aaatagattg ggacacatgc cgttactaat ggatttcata caacaacatt ctatagatcc
     4501 aagcgttata ttttctaaat ttagtaatta ttacgagttc ttagtgagat ataaaaaaat
     4561 agatacatta ttgacggaaa atgaatctaa aaatctggtt ttcttttcaa ggcaaatagc
     4621 acctggattg aaaagaatcg atagtttagt tttagaagaa cttttaaaaa atgaattaac
     4681 atatgatgaa ttaaaaaata aaatgttgaa cgaagttaag gatataacag aagatgatat
     4741 agatacttca ttaagaattt tagatttttc attttacaac gcaggaattg aaaaaatcta
     4801 tggaagtccc attatagaac gtaatgaaag aatgataaga ctaagcgatg catttactaa
     4861 tgctctaagt aatcaaacat ttaatatgtt tttagaagat cttattgaat tatcaaagta
     4921 taacaatgaa aaataccaaa aaggt
//
LOCUS       DALAAR010000126         4905 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000126 DALAAR010000000
VERSION     DALAAR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.131"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            43..915
                     /gene="dprA"
                     /locus_tag="O2458_002631"
     CDS             43..915
                     /gene="dprA"
                     /locus_tag="O2458_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCZ3888467.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1089..3164
                     /gene="topA"
                     /locus_tag="O2458_002632"
     CDS             1089..3164
                     /gene="topA"
                     /locus_tag="O2458_002632"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCZ3888468.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3320..4627
                     /gene="trmFO"
                     /locus_tag="O2458_002633"
     CDS             3320..4627
                     /gene="trmFO"
                     /locus_tag="O2458_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCZ3888469.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1882 a          703 c          907 g         1413 t
ORIGIN      
        1 atagaatatg attttgtatg attacaaaaa ggagttgatt tattgattaa actatttttg
       61 cttaagttat actgggcaca cttttcgact aaacaaattc atcaattttt aatggcatat
      121 cctaatgtaa ttaaagagga gggaagaaaa aaagatagtt atttatgtga atgggtgaat
      181 agggaagaaa atgttcattt attacgtaaa tactatgctt ttataaaact tgatcataac
      241 gatattatta aagaactgca gaaattaaaa gtaagttaca ttacatatat ggattctgaa
      301 tacccagtgc tattaaaaga aatatatcaa tttccattac ttcttttcta taaagggaac
      361 atcaaattaa taaataatat gcatcatttg gcagtagtag gtgcaagaga ttctacaagt
      421 tatacccaac agtctttaga atttttatta tcaaatgata aaagcaaata tttaacaatt
      481 gtttccggcc ttgctcaagg agctgatgca atggcacatc aaatagcttt aaaatacaat
      541 ctccctacaa ttgcagtttt agcctttggc catcaaacac attatcccaa aagtacatta
      601 gcattaagaa ataaaataga agaaaaaggt ttagttatat ctgaatatcc accacataca
      661 ccaatcgcta aatatagatt tcctgagcgc aatagaatta tcagcggttt gtcaaaaggg
      721 gttttaatta ctgaggctaa ggaacaaagt ggcagtcaca tcacgataga ttttgcatta
      781 gagcaaaata gaaatgttta tgttttacct ggatctatgt ttaatcctat gacaaaaggt
      841 aatttattac gtatccaaga aggtgctaag gtagtattaa acgctaatga tatatttgaa
      901 gactactata tttaaaacta aaaataacga gttgcttcat atttcttttg tttccctttg
      961 taatttattt tttaataaaa tctataaaaa atagacaggg aaaatgattt gtttagatat
     1021 aaaacgttga caaaagcaaa attaagcgtt tatcatttat ctttagtaat tagattagcg
     1081 agggggaaat gacattggca gataatttag tcattgttga atcgcctgca aaagcaaaaa
     1141 ccattgaaaa gtatttaggt aagaaatata aagttatagc ttcaatggga cacgtcagag
     1201 acttaccaag aagtcaaatg ggtgtcgaca ctgaagataa ttacgaacca aaatatataa
     1261 caatacgcgg aaaaggtcct gttgtaaaag aattgaaaaa acatgcaaaa aaagcgaaaa
     1321 acgtctttct cgcaagtgac cccgaccgtg aaggtgaagc aattgcttgg catttatcaa
     1381 aaattttaga gcttgaagat tctaaagaaa atcgcgttgt tttcaacgaa ataactaaag
     1441 acgctgttaa agaaagtttt aaaaatccta gagaaattga aatgaactta gtcgatgcac
     1501 aacaagcgcg tcgaatatta gatagattag ttggctataa catctcgcca gttctttgga
     1561 aaaaagtaaa aaaagggttg tcagcgggtc gagttcaatc tgttgcactt cgtttagtca
     1621 ttgaccgtga aaatgaaatt cgaaacttta aaccagaaga atattggact attgaaggag
     1681 aatttagata caaaaaatca aaattcaatg ctaaattcct tcattataaa aataaacctt
     1741 ttaaactaaa aacgaaaaaa gatgttgaga aaattacagc tgcattagat ggagatcaat
     1801 tcgaaattac aaacgtgact aaaaaagaaa aaacgcgtaa tccagcaaac ccatttacaa
     1861 cttctacatt acaacaagag gcggcacgta aattaaactt taaagcaaga aaaacaatga
     1921 tggtcgcaca acaattatat gaaggtatag atttgaaaaa acaaggtacg attggtttaa
     1981 taacatatat gagaaccgat tctacacgta tttcagatac tgccaaagtt gaagcaaaac
     2041 agtatataac tgataaatac ggtgaatctt acacttctaa acgtaaagca tcagggaaac
     2101 aaggtgacca agatgcccat gaggctatta gaccttcaag tactatgcgt acgccagatg
     2161 atatgaagtc atttttgacg aaagaccaat accgattata caaattaatt tgggaacgat
     2221 ttgttgctag tcaaatggct ccagcaatac ttgatacagt ctcattagac ataacacaag
     2281 gtgacattaa atttagagcg aatggtcaaa caatcaagtt taaaggattt atgacacttt
     2341 atgtagaaac taaagatgat agtgatagcg aaaaggaaaa taaactgcct aaattagagc
     2401 aaggtgataa agtcacagca actcaaattg aaccagctca acactataca caaccacctc
     2461 caagatatac tgaggcgaga ttagtaaaaa cactagaaga attgaaaatt gggcgaccat
     2521 caacttatgc accgacaata gatacgattc aaaagcgtaa ctatgtcaaa ttagaaagta
     2581 agcgttttgt tcctactgag ttgggagaaa tagttcatga acaagtgaaa gaatacttcc
     2641 cagagattat tgatgtggaa ttcacagtga atatggaaac gttacttgat aagattgcag
     2701 aaggcgacat tacatggagg aaagtaatcg acggtttctt tagtagcttt aaacaagatg
     2761 ttgaacgtgc tgaagaagag atggaaaaga ttgaaatcaa agatgagcca gccggtgaag
     2821 actgtgaaat ttgtggttct cctatggtta taaaaatggg acgctatggt aagttcatgg
     2881 cttgctcaaa cttcccggat tgtcgtaata caaaagcgat agttaagtct attggtgtta
     2941 aatgtccaaa atgtaatgat ggtgacgtcg tagaaagaaa atctaaaaag aatcgtgtct
     3001 tttatggatg ttcgaaatat cctgaatgcg actttatctc ttgggataag ccgattggaa
     3061 gagattgtcc aaaatgtaac caatatcttg ttgaaaataa aaaaggcaag acaacacaag
     3121 taatatgttc aaattgcgat tataaagagg cagcgcagaa ataatatttt tatttcctag
     3181 atacatttta agattgttaa atagaatcat tagtgaatct tattttaaag atagtaaagg
     3241 attaatctaa ataagtgcgg ataatataaa cataacaaca taattaaaag acataaatga
     3301 caataaaagg agtatagaaa tgactcaaac tgtaaatgta ataggtgctg gtcttgccgg
     3361 ttcagaagcg gcatatcaat tagctgaaag aggaattaaa gttaatctaa tagagatgag
     3421 acctgttaaa caaacaccag cgcaccatac tgataaattt gcggaacttg tatgttccaa
     3481 ttcattacgc ggaaatgctt taactaatgg tgtgggtgtt ttaaaagaag aaatgagaag
     3541 attgaattct ataattattg aagcggctga taaggcacga gttccagctg gtggtgcatt
     3601 agcagttgat agacacgatt tttcaggtta tattactgaa acacttaaaa atcatgaaaa
     3661 tatcacagtt attaatgaag aaattaatgc cattccagat ggatacacaa ttatcgcaac
     3721 aggaccactt actacagaaa cccttgcgca agaaatagtg gacattactg gtaaagatca
     3781 actttatttc tatgatgcgg ctgctccaat tattgaaaaa gaatctattg atatggataa
     3841 agtttactta aagtcccgtt atgataaagg tgaagctgca tatttaaact gtcctatgac
     3901 tgaggatgaa tttaatcgct tttatgatgc agtattagaa gctgaagttg cgcctgtaaa
     3961 ttcatttgaa aaagaaaaat atttcgaggg ttgtatgcct tttgaagtaa tggcagaacg
     4021 cggacgcaag acattactat ttggaccaat gaaaccagta ggattagaag atccaaagac
     4081 tgggaaacgt ccttatgcgg tggttcaatt aagacaagat gacgctgctg gtacactcta
     4141 caatattgtt ggcttccaaa cgcatttaaa atggggagct caaaaagaag tcattaaatt
     4201 aattccaggc ttagaaaatg ttgatattgt tagatatggt gtgatgcata gaaatacctt
     4261 cattaattca ccggacgtat taaacgagaa atatgaattg atttcacaac caaacataca
     4321 gtttgcggga caaatgactg gtgttgaagg ttatgtagaa agcgcagcta gcggcttagt
     4381 tgcaggtatc aatcttgcgc ataaaatatt aggcaagggt gaggtagtat ttccgagaga
     4441 aacaatgatt ggaagtatgg cttactatat ttctcatgct aaaaacaata agaatttcca
     4501 acctatgaat gctaacttcg ggttattacc atctttagaa actagaatta aagataaaaa
     4561 agaacgctat gaagcacaag ctaatagagc tttggattac ttagaaaatt tcaaaaaaac
     4621 tttataaaat agttagaaag actagatatg ctattcattc ttaagtcatc aacgagtaag
     4681 taatgacttt ctaaatggaa aatacttatc ctagtctttt taattttgga attgttacgt
     4741 atttctgaca atttagaatt cgcattcaaa aaatatctaa ataaataaca cgcaataagt
     4801 tgattgatgt aacatgtaag agaatgtttt aaataaactt tatttaaaag gcaatgaaat
     4861 aataaatggc aaggctatta ataaagactt ttagtaatta attta
//
LOCUS       DALAAR010000127         4833 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000127 DALAAR010000000
VERSION     DALAAR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.132"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..566)
                     /locus_tag="O2458_002634"
     CDS             complement(<1..566)
                     /locus_tag="O2458_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ3888470.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
     gene            644..745
                     /locus_tag="O2458_002635"
     CDS             644..745
                     /locus_tag="O2458_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888471.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(830..1513)
                     /locus_tag="O2458_002636"
     CDS             complement(830..1513)
                     /locus_tag="O2458_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCZ3888472.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1879..2577)
                     /locus_tag="O2458_002637"
     CDS             complement(1879..2577)
                     /locus_tag="O2458_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCZ3888473.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2957..3655)
                     /locus_tag="O2458_002638"
     CDS             complement(2957..3655)
                     /locus_tag="O2458_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCZ3888474.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3999..4694)
                     /locus_tag="O2458_002639"
     CDS             complement(3999..4694)
                     /locus_tag="O2458_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCZ3888475.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
BASE COUNT         1458 a          855 c          585 g         1935 t
ORIGIN      
        1 attaggaatt catatgcatc acctaacata tcaatttcca tgtcactgtg aacgaatggt
       61 aagtcgtcaa gattaaccat gactttagag attaaagcag tacgttcttt gacattgtta
      121 cctagtcgcg ttgaactcaa atccatatcg ctgaacagac cgataaagtc attttcactt
      181 tcttcaccta atgttgatgt ttcaacttta cgaattgccg tcgccaggtg ttcgatatcg
      241 aaatcttgcg tttcaatttc acgaatcatc gcactgaata aatcttctgg ctcaatgaag
      301 taaccgactt ggtcaattaa ttctgctttt aagtcttcac ggtattcttc gtctgcccat
      361 gcttcttgat acgtgatgtc ttcacctgac aaggcatctg catattcttg ttccgctttt
      421 tcagataaga agcgatagaa aatcaagcct aaaatgtaat tacggaattc actcgcatcc
      481 atattccctc ttaaatcatt cgcaatcgac cataattttt tatgtaattc agcttgttgc
      541 tgacgttgtt tttcagtaat agacatgtga ttcctccgcc tttgaataag taatttatct
      601 ctttgtgtaa tagatttatt ataacatttg gttatgttgc gatgtggaga attttgatgt
      661 tggtggtgga aattttacct ttatggagat tgggatagga ttcatgacac acaaaccaaa
      721 acggcttaac gacgatatcg attgatgcat cctttttgta taagctatgc gcaccaattt
      781 acatttcacc gttaagccgc ttcaattact tttaattatt cgtaatgatt tactttaagt
      841 taacttcaat atctttaatt tgtttgcttt ctatgacttc ccccatatat ttaaatttta
      901 atttagatct taaatcaagg ttataaaaac tgccatcttt caagctaatt ttgaccctac
      961 catcttttga gattgtttta taaagtccgt atttttcaat tagatgcttt ctcaacttat
     1021 aatccagttc ttttagtgtt attttttctt ttttaatgta ataagggtaa cctttcacaa
     1081 aagcatcttc acccttttct tttgaaatat ttaatattgg tgcactgaca actccagatg
     1141 ttttattatt ctgtattaca ccaccaacag tataaaatat atcgtgctta tctctttttt
     1201 cttgaacaaa gaaaacatct aacccctcat aactacgctg ttgaaattta cttttatcat
     1261 ttccaggcag taaaacttga atcttaatac ctctaaactt aaaacgtaag ttgtttttac
     1321 catgtttcaa agcactaata tttttcattt ccatagtctt tccagtgtag tatcggtata
     1381 atgcttcctt gtcatgttta tttactgact tttgattttg ttttgcatga cctgaatgaa
     1441 cttctgttgt taaagttcct gttgttaaaa ttcctaaagc taatgtcgct tttgctaatg
     1501 ctgtaaattt catatttatt tgctccaatc ttaatatatt ggattgtttt cattacgtaa
     1561 tttgaatcat acatttttat tataacgtgc atcgtttcat acgataatta acctcgattt
     1621 aaatatactt gaattattct taatgaaaaa caaatatatt ttaatgttca ggttaaatga
     1681 ctttcattat ttcatttagt tgttttaccg gattatcgtt tactacaact gatgatgtta
     1741 ctctgttctg ctctttgaat tacaaaaaca acttaacaac agtaatgtat ttaacacagt
     1801 tccttatata aggacacaaa tgccaattca cattttgtcg ttaagccgct tcttaagcta
     1861 ttatgctatt aactcgaact aattcaaatt cacttcaata tttttaattt gcttactatc
     1921 cattacatca aacatacgtt caaaacttaa tttttcactt aaatcaattt catgcttctt
     1981 ttcgtctttc atattgataa caattctacc tttatcagac gttcctttat acaatctata
     2041 tttttcgatt aagagttttc ttattttaaa gtccagttcc ttcaatgata cttcttcctt
     2101 ttgaataaaa aacaactcgt ttatcgaaaa gccattttta gcatcaactt ttttaacttg
     2161 tagacttgga ttacttacaa atccaaacac tgatctcaca tttttctttg ttataccgcc
     2221 aacgctatat atgccacctt ttatatctat taattcaggt actgcaaaga catcaaggcc
     2281 atgtgttttt tctttatatt tatttttatc gtcaccaagt aaaaatactt taaaagcttt
     2341 atttcgttgt ttaaagttta aaacgttaga gccatagtaa tcttcgcttt taacactaat
     2401 gtttgttggt tcaaaacttt cttcagaata gtattgatgt agcatattga tataatattt
     2461 gcgttgtgtc tcatcaagtt ctactttcgc gtgaccagtt tgactttctg ctgtaaacac
     2521 acctgtagtt aatattccta atgctaatgt tgctttagct atcgttgtta atttcatatt
     2581 tttttgctcc aatcttaatg tattggattg ttattattac gtaatttgaa ctatctgtct
     2641 ttattataag gtgcatcaaa acagactgac attaacgctc atttaaataa acttgaatca
     2701 ttattaatga aaatcaaacg tattttaaca ttcgtattaa ctgattttta atagcaacat
     2761 aaatattttt ttggtgaatc attgttcttt ccgattagtt ttaaatctta agctattggt
     2821 tataaagtgt acaaaaaagc agcttaataa cgtagtcaat gttcgatatg tttttattat
     2881 aaaaacacgt atcaaaatca ttaaacatgg gattattaaa ccgcttctta agctttcatt
     2941 ctatatatat cattgattat ttcaaattca cttcgatgtt tttaatttgt tcactattaa
     3001 tgacatctgc catacgctcg aaatctaatt tatcacttaa atcaatttca tacttatttt
     3061 catctttcat attaataaca attctacctt tatcagctga cccttcatac agtttgtatt
     3121 ttttaatcaa cagttttctt attttaaaat caagttcctt caatgacact tcttcctttt
     3181 gaataaagaa aaattcatca atagaaaaac cgtctttatc gtctattttt ttaactagta
     3241 agttcggcgt tcttagagac tcaaatattg attttacgtt tttctttgtt acaccactaa
     3301 cactaaatat tcttccatct aaatctacta attctggtac cgcaaagaca tctaaaccat
     3361 gtgttttttc tttgtattta ttttcatctt ttcccaataa aaacacttgg aacttttgat
     3421 tttgttggtt aaaaagcaaa acgttagagt cataataacc ttgactttgg ccactaacat
     3481 ttgttaactc ataactaggt cctgaatagt attgatgtaa cttgtttgta tcatataaac
     3541 ggttcatttt ttcatacttt ccttttgcat taaccgattg attttctgtt atcattacac
     3601 ttgttgttaa tattcctaat ataaatatcg ctttagctat cactgtaaat ttcatattca
     3661 tgtgctccaa tcttaatata ttggattgct tttattacgt aatttgaata atacaccttt
     3721 attataaagg gcattgagac agactgacat taacactcgt ttaattaaac ttaaatcatt
     3781 gttaatgaag agctaattaa tattcaatgg gcatcacttt caagagcaat gtaaattttt
     3841 agtatgctca ctcataacct gcgttataaa acgtaaaaaa aagcctaacg acaaggttgt
     3901 tttaaatacg ctccttaatg taaggtgtac gtatctattc aacatttcat cgttaagctg
     3961 cttcaaattt attactttag agtcattgat tacttacttt aaatttgttt caaagtcact
     4021 tcaatcttat taatatcctt actattcaac acatcaccca tgcgctcgaa ttgcaattta
     4081 tcacctaaat caatttcttg cttttctcca tctttcaaat tgatagtgat cttaccgtat
     4141 ttagtcgtac ctttatataa accataattt ttaactaaat gttgtctaat tttgaaatca
     4201 agttctttca gtgaaacttc ttctttatta attgaaaatg agtcaattga tgcatctaaa
     4261 tttccgccat acactttatt aacaaataaa tgtgtattag tttcagaaga tttgttattt
     4321 ttcttagtca caccaccaac agtagataat ctaccgtttg gatcaattaa ttcttttacc
     4381 acaaagacat ctttgccttc aatgccttct ttatatttct ctttatcttt acctaataag
     4441 aataattggt gattttgatt ttcttggtta aagcgtacaa cgttagaacc gttataattt
     4501 tcaaccttac cactaatatt actgaattca aaactttctg atgagtagta tcgatgtaag
     4561 tctttaatat catataaatg ttgtactctc tcttgctttt cttttgcttg aactgcttgg
     4621 ccttctgatg taatcacacc agtagttaat aagcctaatg ccaatgttgc tttagctaac
     4681 gtttttaatt tcacagtact attctcccaa tctatttata aatttgtctt aatatatttt
     4741 tatatgatta attcaattac gtaatacgaa caatctacta tcattatata agtgagatac
     4801 tgcgctatga attaacctct ttttaactat ttt
//
LOCUS       DALAAR010000128         4717 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000128 DALAAR010000000
VERSION     DALAAR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.133"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            381..1418
                     /locus_tag="O2458_002640"
     CDS             381..1418
                     /locus_tag="O2458_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ3888476.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1469..2299
                     /gene="sph"
                     /locus_tag="O2458_002641"
                     /pseudo
     CDS             1469..2299
                     /gene="sph"
                     /locus_tag="O2458_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2537..3553)
                     /gene="lukG"
                     /locus_tag="O2458_002642"
     CDS             complement(2537..3553)
                     /gene="lukG"
                     /locus_tag="O2458_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCZ3888477.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3575..4630)
                     /gene="lukH"
                     /locus_tag="O2458_002643"
     CDS             complement(3575..4630)
                     /gene="lukH"
                     /locus_tag="O2458_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCZ3888478.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         1611 a          746 c          699 g         1661 t
ORIGIN      
        1 aattgataag gtaaaaaaat taaaaggtaa tagttacgct tgcataaatt ctttccagcc
       61 gattagtaag tttcgcataa gaaaagtttt accccaaaaa attaaaaatc cagtaataga
      121 ttcttcggat attatgttac tgataaatag aattaataat aatatattgc agattcctga
      181 tataagatga tataatttta atatattaaa ggtttatcct ttaaaacacg tatatattcg
      241 ttaccatttt tggtaattaa ccatgtaatc ttataactat aagtggcgtc tgtattttat
      301 acaggcgtct ttttttatac aattttcatg ggtagcccgc ctacccttat tattttttgc
      361 caattttgag gagggaacgc atgaaaacac gttgttacga tggtaaaaaa tggcaatatg
      421 aatttaagta tgaaggaaaa agataccgta agaaaggttt tagaacaaag cgtgaagcta
      481 attctgctgg actagacaag ttaaatgagt taagaagtgg ttttaatata gataactata
      541 taactcttga agaatacttc gaaaattgga ttaaaacgta taaacaacct gttgttaaag
      601 aaaataccta ccgtcattat agaaatgcat tacaacatat acaaaaacat aaaataggta
      661 aaatggagtt atcaaagata aatagacaag tttatcagaa attcataaac gattattcaa
      721 aagaacacgc aaaagaaact ataagaaaaa caaacggtgc tattcggtca gctttagatg
      781 acgcattata tgatgggctt atttttaaaa atcccgctta taaagttaat tataaagccg
      841 gaaaacctac gaagtcagaa caagaaaaat tcatctcggt aactgaatat gaaatactaa
      901 aagatcacgt cagaaagaag agaactcgtt catcattagc gctattcata atgatttgta
      961 cgggttgtcg tgtcagtggt gcaagaaata taaagattga gcatatcaac caagtgaaaa
     1021 acactatatt tattgacgag cgaaaaaccg atacttcccc tagatatatc agtatcgcta
     1081 aatctgatat gaaacacatt atggacgtca taagtacatt tgcaattagc tatgatggtt
     1141 acattttcaa agaagccgga tctataatta accttcatgc tatcaataat gctttgaaat
     1201 cagcctgtag agtcaataat atatcaatta ttacatcgca cgcattaaga cacactcatt
     1261 gttcttattt actagcaaaa ggtgtatcta tacattacat ttctaaaaga ttaggtcata
     1321 aaaatatagc aataactaca tccgtgtatt ctcatttgtt agaagaaaaa tttaatgaag
     1381 aggacaaaaa aacaactaaa attttagaaa gtatgtaatt tagggaccca ttagggactc
     1441 caaacccaat aaatactgtt gttacaaggt ttctatgtat ccaaactggg ggcaatataa
     1501 acgcgctgat ttaatcggac aatcttctta tattaaaaat aatgatgtcg taatattcaa
     1561 tgaagcattt gataatggtg catcagacaa attattaagt aatgtgaaaa aagaatatcc
     1621 ttatcaaaca cctgtactcg gccgttctca atcagggtgg gacaaaactg aaggtagcta
     1681 ctcatcaact gttgcagaag atggtggcgt agcgattgta agtaaatatc ctattaaaga
     1741 aaaaatccag catgttttca aaagcggttg tggattcgat aatgatagca acaaaggctt
     1801 tgtttataca aaaatagaga aaaatggtaa gaacgttcac gttatcggta cacatacaca
     1861 atctgaagat tcacgttgtg gtgctggaca tgatcgaaaa attagagctg aacaaatgaa
     1921 agaaatcagt gactttgtta aaaagaaaaa tatccctaaa gatgaaacgg tatatatagg
     1981 tggcgacctt aatgtcaata aaggcactcc agagttcaaa gatatgctta aaaacttgaa
     2041 tgtaaatgat gttctatatg caggtcataa tagcacatgg gaccctcaat caaattcaat
     2101 tgcgaaatat aattacccta atggtaaacc agaacattta gactatatat ttacagataa
     2161 agatcataaa caaccaaaac aattagtcaa tgaagttgtg actgaaaaac ctaagccatg
     2221 ggatgtatat gcgttcccat attactacgt ttacaatgat ttttcagatc attacccaat
     2281 caaagcctat agtaaatagt gctcaactaa ctaataactt gcttcgttct aaaaggacga
     2341 agcgagttat attgttaaaa tttgaattga cttacatttt aataaaatca tcttaacaac
     2401 tttaattttt cattaataca agtctttact ctacactcaa acaagattca tacactgcac
     2461 gtcataataa atctatctat tcaaatataa ataaaagtta cctactacat tctatgtagc
     2521 aggcaacttt tattacttat ttcttttcat tatcattaag tacttttaca aacttcacat
     2581 tatgtgtctt ccaatcaact tcatataatg ctgataattt ttcttctttt ttatctacat
     2641 ggttttcacc agaccaatag ccccagaaac catggcgatt ccaatctatt ttaaactcat
     2701 ccattgatct tttataatga acaacaaatt gtgatttacc tttgtctttt ttatcatgtg
     2761 acataacagc taaaaattct ggattaaacc cttcagacac agttacaggc attttgtctt
     2821 taggtgtgaa attatctttc gcccataaat ttccatttcg tgttaaagaa aaaatttcac
     2881 ttttagttct attatcacta tcattagtta attgtctcgt atggtcatgt cccatattat
     2941 ttatcaaatg tgcttctact ttccaaccta cacctttatg tgacgtagat tgatcaagta
     3001 atgtacgata tgatggttgt tgataactaa tcgtctctga ataattactc tcttttgtaa
     3061 tatttccagt taagcctcca cgattaatcg aaaaatctcc acctgtttta taaccatacg
     3121 tatatttaac ttctcttgat tcatcctgat tttttggtgc aaagtcagtc acatttgtat
     3181 tgttgttgtc atcaacattt tgaattgaaa ctgaataaga tccaggccat cttaatgtac
     3241 tattccaata accatttggg tctaaaattc tcaaaccact accaattgta ccttttgctt
     3301 taataaatac tgtttcttta tcataattag gttcagttaa gaaattaaat tgtaagcttt
     3361 gagtaatatt tttttgacta tcacttgttg tagctgtacg tgtatacatt ttagtatcac
     3421 catcaagatt cttctcagaa acttgtttga tttcagaatt aatctttgca aatgaagtag
     3481 ctggtaatac agtgaaagta gtcgatagtg ctaacgtaca aattgtgata tttttacata
     3541 gttgtttaat cattagtaat ccgccctttc aatattatcc ttctttataa ggtttattgt
     3601 catcagaata tttatcaacg actttaactg ttttattttt ccaatcaact tcataagtga
     3661 caattaatct ttgaccatct ttattttttt ctaaaattgg aggtgcataa tgtattccag
     3721 gtctgttttt caaaatatct tgatttcgtg tgtatgttac ttcaaattgc gttttctcat
     3781 ttgacttttc attagataaa taagttaaaa attctggatt aaagccactt cttactaatg
     3841 ctgggtatct atattttgaa gcaaagctta gttcagggtt ttctacagta gcaattctcg
     3901 tatttctata gaataataat tcatcatttc tatttttcac ttctccacca tacttcaagt
     3961 cattcgcaat aactgaccag tgtacatgcc agttattatt tttaccgctg gcaattgtgt
     4021 cataattttg ctgattataa ctaatcgttt tggagtagct atttgatgaa gttcgtccaa
     4081 taccttttgt tgaatcgaat ttaccacctg agctatatga aaatgtactg tctacttttg
     4141 cagttgaaat tttatttttc ggcaattggt ctaatatttc agttttacga tttcttttta
     4201 cttgaaaatc tacatggtac tcacttggat actttaacca atttgaattt ttttcttctt
     4261 tatgagattc aaactttaaa tttgaatgaa ttgagccttg ttttttaaca agtaatacat
     4321 tcttgtcata agttggatca tcgataaagt cgaattgtaa attttggagt atatttgttt
     4381 tctcatcata tactgtttca gttcgttttg tgattttacc gtttttccca atatcatccg
     4441 gtgctgttga atttttatct ttattagtaa cattacgttt gtctttttgt tgagacttat
     4501 caacatgttc tttcttattt tggtcttgag agtctttatg agctgaattt gcttgagtcg
     4561 ttgctgctga taacaataaa attgcacatg ataatgatga cgctattaaa acacgttttt
     4621 tatttttcat tgttataacc ttctttcgta tgattgatat ttgttgatat gtatcgacat
     4681 gtgaataata tcacaaaaac agagaatata tatttaa
//
LOCUS       DALAAR010000129         4685 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000129 DALAAR010000000
VERSION     DALAAR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.134"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            13..189
                     /locus_tag="O2458_002644"
     CDS             13..189
                     /locus_tag="O2458_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888479.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            453..833
                     /locus_tag="O2458_002645"
     CDS             453..833
                     /locus_tag="O2458_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3888480.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            830..1726
                     /gene="pmtA"
                     /locus_tag="O2458_002646"
     CDS             830..1726
                     /gene="pmtA"
                     /locus_tag="O2458_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCZ3888481.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1727..2407
                     /gene="pmtB"
                     /locus_tag="O2458_002647"
     CDS             1727..2407
                     /gene="pmtB"
                     /locus_tag="O2458_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCZ3888482.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            2404..3276
                     /gene="pmtC"
                     /locus_tag="O2458_002648"
     CDS             2404..3276
                     /gene="pmtC"
                     /locus_tag="O2458_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCZ3888483.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            3276..4016
                     /gene="pmtD"
                     /locus_tag="O2458_002649"
     CDS             3276..4016
                     /gene="pmtD"
                     /locus_tag="O2458_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCZ3888484.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(4082..4645)
                     /locus_tag="O2458_002650"
     CDS             complement(4082..4645)
                     /locus_tag="O2458_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ3888485.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
BASE COUNT         1616 a          594 c          773 g         1702 t
ORIGIN      
        1 aggtgaaata aattgaaaca tctaacaaaa atatttgtag tacttgcaat tattttattt
       61 attattggtt attatttaca agcaactaat catgaaagcc aaggtataaa attattatta
      121 gcagcgatta tgtttatgat ttgtgctttt ataagtagaa gtaatgatcg acgtaaaaat
      181 agtaaataga attgactgtt taaaacgatt gtttatgttg acgctacggt tgtcacgtag
      241 agattaaaga cattcgtttt agcagtcttt tttattgaag tagacaatgc aagattaaat
      301 ggtagtgtca tttcatttta ttttattcaa ctgctagtaa tacaatactc agtgttattt
      361 ctgaagactg gttgataatg agacatgcat atttacaaaa ctgtgtatat tgtgtatatt
      421 gtatatatac agaagttgat agggggacgt tgatgaaaat aattttaaaa aacaatagtg
      481 attttccgat ttatgaacag attaagcaac aagtaaaaca aaatatttta aagggacatg
      541 ttgctcctgg agagcatttg ccgtcaatga gagaacttgc caaagatctt caagtaagtt
      601 tgattactac caaacgtgct tatgaagatt tagagaaaga cggttttgtt acaacaatta
      661 gaggaaaagg gacctttgtt aaggagcaag atagttctat tttaaaagag aaacaatttt
      721 ttaccattga aaatttggtt aaagaattgg ttaatgaagc gcaagccatc gaaatgtcac
      781 ttgaggaact gcaggatatt ttaacgttca tttatgagga ggaatcatca tgaatgccat
      841 agaattaagt aatgttaatt attcaagtga tcagtttaat ttaaagaata tctctttcaa
      901 agttcctcaa ggattcgtca ctggttttat tggtagaaat ggcgctggta aaaccacaat
      961 aataaggtta attatggatt tgtatcaacc acaaactggt gttattagag tattagaaga
     1021 ggatatggct ctcaatccga tagagttaaa gaatagaatt gggtttgttt actctgaaaa
     1081 ttattttaat gagagatgga ctacgaagca acttgaaaaa atgattgccc ctttttatcg
     1141 taaatgggac catcaagtat ttgaatttta tcttgaaaag ttcgatttac caatcaataa
     1201 atcaataaaa acattttcga ctgggatgaa aatgaaatta tcactagcag ttgcattttc
     1261 acatcatgct gaattgtata tttttgatga gcctacttca ggtcttgatc ccttagctag
     1321 aaatgagcta ttggagataa ttcagcaaga attaattgat gaaaataaaa caatattcat
     1381 gtcgacacac attatctcag atttagaaaa aatagctgat tatatcattc acttaagtga
     1441 cggtgaagtt atcttaaatg gttcaaaaga acaactgtta caaagatatc aagttgtgag
     1501 tggtgctatt gaggaccttg atgatgaatt agcatctcta cttatttatg aagaacataa
     1561 gcgtacggga tttataggat taacagaaca tgcgcaagta tttaaagaaa ttttaggtca
     1621 taaagtgaat atcactacac cttctattga gaacttaatg gtttatcttg aaaagagaaa
     1681 acctaaatat catgaaaaca tcaaattgat ggaagaaggt ttttaaatga aggcattgtt
     1741 aattaggaat tttaaattac gacgatatac acttatcatc tatgttctat tattaacctt
     1801 atatcctttt tatataatgc ttgactcaac aaagttcttt tacttattgc aatcttttat
     1861 aagtccaaca atattgatta tttggatttt agatgctggt cacttattta gactcaatcg
     1921 tagattaggt ggtaatgatt catattattt ttatatgagt ttaccagtgt caaaaaaaca
     1981 attgcttaat gccaactata ttacatgcat tgttttaaca ttaattggaa cgcttgttat
     2041 tagtttatat gcttatgaag cagatgtgat tgaaccaaat tcaatctatt tttcaactgc
     2101 atatgcattt gtcatatcta acttcttgtc tataccaatt gcatttagtc aatttacaga
     2161 attgcgtaga gtcaaagtgc catatggtat atacgtgttt actattatca ttttagttcc
     2221 atttttattt tcaattgcaa tagtattggt gaattatttt gttctaagtc aatcatcatt
     2281 cccagattta tattcatata ttttaaatat tggttttcta attataagca ttgttatact
     2341 tattgttaat tattttaaac agctcaataa aataaatact agaaaattta aaggaggcag
     2401 tcgatgaaat tagaacatat tacaaaaaaa tacggctcaa atgtcgtttt aaatgatatt
     2461 gattttgact ttggcgatag tagaattgtc ggattaatag gaaaaaacgg tgttggtaaa
     2521 acaaccgtta tgaaagtaat gaatggtaat attattaaat ttgatggaaa agtagatatt
     2581 gataatgcag ataatatcgg ttttttaatt gagcatccta aattatatga taataaatca
     2641 ggattgtata acttgaaatt atttgcacaa gtattaggta agggttttga taaagcatac
     2701 acagacaaaa ttatagatgc atttggtatg agaccttata ttaaaaagaa agttaagaaa
     2761 tattcaatgg ggatgaagca aaagttagca attgcagtat ctttaatgaa taaacctaaa
     2821 tttttaatct tggatgagcc tacaaatggt atggatccag atggctcaat tgatgtgctg
     2881 actacaatta agtctttagt aaatgaactt gatatgagaa ttctaatatc aagtcataag
     2941 ttagaagata ttgaattaat ttgtgataga gctgtatttt taagagacgg acattttgtt
     3001 caagatgtaa acatggagga aggtgttgca tctgacacaa cgatagttac tgttgatcat
     3061 aaagactttg atagaactga aaaatatctt gcagagcatt tccaattaca aaatgtcgac
     3121 aaagcagacg gacatttaat gatcaatgca caaaaaaatt atcaagttat actaaaagca
     3181 ttatctgaat tagatattta tccgaaatat attgaaacac gtaaaagttc attgcgtgat
     3241 acgtacttca atataaatca aagaggtgat aaataatgag aattttaaat ttagttaagt
     3301 atgattttta tagtatattt aaaagtcctt taacatattt agcgatacta gtcgtatcta
     3361 gtttgattgc aactcaaagt atacttatgg caaattcgat ggataacccg aaacatatta
     3421 ttgtctatgg atctgtattt gctgcagcaa aatggttatt gttaataatt ggattaatgt
     3481 ttgttgttaa gacaattacg cgtgattttt cacaaggtac aattcaacta tatatgagta
     3541 aagttaaaac acgcgttgga tacattattt cgaaaacaat ttcaattatt ttaatttcaa
     3601 tattatttgc attaattcat tatgtgattt tgattgttgt gcaggcatct agtaatggaa
     3661 aaaatttggc gttttctaaa tatgtagata atttatggtt cttcctaatc tttttactat
     3721 tctttggctt gtttttattc ttaatcacac ttgcatcaca aaaaacagca atgatatttt
     3781 cattaggtgt atttttagta ctcattgtac cgtttattaa accttttatt acatttatcc
     3841 caagatacgg tgaaaaagtt ttagatgctt ttgattatat cccttttgct tacttaactg
     3901 ataaaatgat tagctctaac tttgatttta gcaattggca atgggtaatt tcattaggtt
     3961 ctatagtgat attcttcatt ttgaatatct tatatgtcgc taaaaaagac atttaataaa
     4021 aataattttg aggttgggaa ttttaaattt tcccaacctc aaagtttgtc ttattgtaaa
     4081 tttatttatt ttctaattta tttaggatgg aattataaac tgctttccaa aatgaagcgt
     4141 cagttttata gcggtttgat ataactaagt gtgtttcttt ttctaaatct gcatagtctg
     4201 gatgatcttt gctcggtaat ttatcagcac gaacatcagt tacaaatttt tggacttcat
     4261 ttgctcttgg tccccaaact gtttcttgtt cgaattgatc attcaagaat acgaagatag
     4321 gaattgcacg tgatttacca tttgttaaat attgatcgat cagttttgta tcatcatctc
     4381 tatggaacac gcgtacttct aaatttaatg cttcactgat gtgttttaga attgggagat
     4441 tcatcattgc atctccacac cagtcttcag taattactaa tactttagaa taattcatct
     4501 cttttatttt tttgatgcgt gaatcatctt ctggtaactc aaatgattga tagatactga
     4561 gaacggtatc ttgatttgtc ttcattccat caatgtattc atttaagggt tggctatttt
     4621 tgaaataagt ttctaaattt gtcattgtaa aaacctcctt tagcatttac aacattatac
     4681 caatt
//
LOCUS       DALAAR010000130         4621 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000130 DALAAR010000000
VERSION     DALAAR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.135"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            222..872
                     /locus_tag="O2458_002651"
     CDS             222..872
                     /locus_tag="O2458_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCZ3888486.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            953..1948
                     /locus_tag="O2458_002652"
     CDS             953..1948
                     /locus_tag="O2458_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCZ3888487.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            2024..2650
                     /locus_tag="O2458_002653"
                     /pseudo
     CDS             2024..2650
                     /locus_tag="O2458_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2691..3035
                     /locus_tag="O2458_002654"
     CDS             2691..3035
                     /locus_tag="O2458_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCZ3888488.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            3133..3684
                     /locus_tag="O2458_002655"
     CDS             3133..3684
                     /locus_tag="O2458_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888489.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(3902..4543)
                     /locus_tag="O2458_002656"
     CDS             complement(3902..4543)
                     /locus_tag="O2458_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ3888490.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
BASE COUNT         1829 a          723 c          716 g         1353 t
ORIGIN      
        1 tttgttaaat tatgttgtca tgttcgtgtc attttgagtc gtaatacaac tttacaatta
       61 ttaaattaac acttctacaa tccaaaaata tttttaaagt tttgaaaaaa attaaagtat
      121 ttccactact aatttggtaa aatatgatta tgtgatatag tgaaagcgtt ttacacttaa
      181 taactccctc ttaaatgcat ccaggtttta tgtagtaaat catgaaaata acatataaat
      241 atagaggaga tttacctttg aatacagaga acaacaagaa tcaaaaccaa tctgttaaaa
      301 attctgaaag acgcggcatg ttaaaaggat gcggcggttg ccttatttct tttattttat
      361 taataatctt attatcagcc tgttcaatga tgtttagtaa taatgacaat tccactaata
      421 atcaatcatc aaaaacgcaa ttaactcaaa aagatgaaaa taaaaatgaa gataagcctg
      481 aggaaaaatc agaaacagca acagatgagg atttacaatc aaccgaagaa gtacctgcaa
      541 atgaaaatac tgaaaataat caacatgaaa ttgatgaaat aacaacaaaa gatcaatcag
      601 acgatgatat taacacacca aacgttgcag aagataaatc acaagacgac ttgaaagatg
      661 atttaaaaga aaagcaacaa tcaagtaacc atcatcaatc cacgcaacct aagacctcac
      721 catcaactga aacaaacacg caacaatcat ttgctaattg taagcaactt agacaagtat
      781 atccgaatgg tgtcactgcc gatcatccag catatcgacc acatttagat agagataaag
      841 ataaacgtgc atgtgaacct gataaatatt aaacaacaag cgaattgaat tcaaattgta
      901 tttagcttta tgcactaatc acatagtaaa taatgaggga gattttttag gcatgagcaa
      961 tcaattcaaa agcgaagaag agcgaagaca atgggaacaa ttccaagctt tccaaaatca
     1021 acaaaaccaa cagaaccagc aatacggaca aaagaaatct aaaaaaggat ggttctgggg
     1081 ctgtggtggt tgtctagtat tatttatttt aattatcatc ggtatttcag cttgtacagc
     1141 tggtattaca ggtaaccttg gcggaaatag ttctaaagaa acgaacaaaa cccataaaat
     1201 cggtgaaact gttaaaaatg gcgaccttga agtcactgta aattcagtgg aaactatgaa
     1261 atctgtagga ccatctcttg caccaacaaa cgctaaaggt atatttgtcg ttgctgatgt
     1321 gacgattaaa aacaaaggta aagaagcgtt aacaattgat agttcaatgt ttaagctaaa
     1381 atccggtgat aaaacatttg aagcagataa tacaggttca atgtctgcta atcaaagtga
     1441 caatggtagt atagaaaatt catttttctt acagcgtata aatccagata gcactgctca
     1501 aggtaaaatt gttttcgatg tgtcagaaaa catagccaac gcaaaagata aaaaattaga
     1561 agttatttct agtttattta gcgtcaagaa gattacattt gatttatccg atgctaaaaa
     1621 aacatcaaaa gctaaaaaag acaagcaaga tacagaagta gctgttgcga gttcaaatag
     1681 cgataatgta agttatgaag cttcggctac tacacctgct acaacttcta gtgcggatac
     1741 tgattctgaa gatagcgaaa agtctagtaa agatgaggat aagcagaatg cgtctaaaag
     1801 tgataaatct agtgtagaaa aaagtgaatc taatgaggaa actgctcctg tagagcccat
     1861 gccccatagc aaacctacca ctagtgaagc accacctagc caaaatattc acaatgaaga
     1921 tagcatgtac gacgcttcaa cagaataaaa tttcagggta gctcggctac ccttctttta
     1981 cggaaaaatt aattatacat aatcaaatca aggagataaa aaaatgaaat tcaaagctat
     2041 cgttgcaatc acattatcat tgtcactatt aactgcctgt ggtgctaatc aacataaaga
     2101 aaatagtagt aaatcaaatg acactaataa aaagacgcaa caaactgaca acactacaca
     2161 gtcaaataca gaaaagtaaa tgacaccaca agaagccgaa gatatagttc gaaacgatta
     2221 caaagcaaga ggcgttaatg aatatcaaac attaaattat aaaacaaatc ttgaacgaag
     2281 caatgaacat gaatattatg ttgaacatct agtccgcgat gcagttggca caccattaaa
     2341 acgttgtgct attgttaatc gacacaatgg cacaattatt aatatttttg atgatatgtc
     2401 agaaaaagac aaagaagaat ttgaagcatt taaaaagaga agccctaaat acaatccagg
     2461 tatgaacaat catgatgaaa cagatggtga gtcagaagac attcaacatc atgacattga
     2521 taataacaaa gcaattcaaa atgacatacc agatcaaaaa gtcgacgata aaaatgataa
     2581 aaatgctgtt aataaagaag aaaaacatga taatggggca aataattctg aagaaactaa
     2641 agttaaataa tggcatactt tgattaatcg taatttttag gagaaacgct atgataacat
     2701 ttgagaatat acaacaactt gagaaataca ccttaatgac tatgcatggt ctttttaatc
     2761 aacttaaact cggcattatt tcaatcgaaa acgcagagca tacgcttttt acaccttata
     2821 tgttggaaac gctctcttcc ctaggcgtga aagacagcat tgtcgattta attcataaag
     2881 ggactgaatt agaagacttt gcggcattta atttatcaat tgaagacaca gttacagtct
     2941 gtttacaaag aactgaagaa ctattaaaac aatacaaaaa tgtggaattc aatgacaaaa
     3001 tattaatcaa ttggcgtatt atacaagaga aatagacata taaaagtcga atgtaactac
     3061 gtgagtattg attttattct ttgtaaatta caagcatttc atattataaa gtttgaaaag
     3121 aggtatattg aaatggagaa aaatgaatat atagctaaat ataatgaata tagtcaatta
     3181 ttagacgcta catactcgca agctgtagca taccttttaa gtaaatatgg cgctgtaacc
     3241 gatgattatt ataaagaaaa atcatacacg cgatttttaa atggagaaat caaaagtatt
     3301 tcaaaaggaa aatacactag agctagcgaa ggattatatt gccatcatat aagcgaggac
     3361 aaattccaaa atctatctga tctaagattc atttccaaat ttaagtactc atacgacgtt
     3421 caaaagaaag aaaacttagt gtactgtgat ctaatcgagc atttaatttt acatgcaatt
     3481 attacaaaag aatcccatgg ccaatttggt gtagctggat tatgtcaaat gatcaaacca
     3541 acagtcattg attggtacat tggcgaatat aatccaaaac cagcatggat gcaagccacc
     3601 aaagcacgtg cctatttgcc tggaatatta gtagagaaat tactcattaa aattgacgat
     3661 atgttaaaag gaatagaaat ataagatttc cttgagtcta gataaatgat taatgtagat
     3721 ttattttttg ctgttgagat tttgttatag atgtttaaac ctgtaattaa atatatttta
     3781 taaaatagac cacgcatacc tatctataaa cggacaatgt ttataaatga gtttgcatgg
     3841 tcttgaattg tattaaattt cttttggttt taataaatcg actagatttt cacaatattt
     3901 atcaaatatg tattcctaaa ttatacagcc ttaatccagc agctactttc gaaacttcca
     3961 acttagttga tataaggttc aatagtttgt ttcgttcttt ttcagataaa ccagaactta
     4021 aattgatatt attgacttca taaaaattat agactaatgc ctctatttgc tttttaggca
     4081 taagtaagtc gactgaaaac tgatttacgt cgctttcata aatcatttca tgtaaattct
     4141 ttagactatt atcgttacta tctctcatta agtctgtatt tttaaataaa taacggccca
     4201 attcacgagc tattgaaaat cttgtattat taatcgagtg attattatta atatagattg
     4261 ttcttccact taaataaccc gaagtattac cctccatttt aatatatcta acatttaaat
     4321 taagttgaaa taatagcttg tctatgtcaa tagcaaagtg ttcagaagta ataaaaagtt
     4381 gatccatttt gtcctttata aatgcctgaa ataatcgaac tatttttgat tctaaaatat
     4441 cttcataatg aactttctca ataactttca attgattccc caccctaact aaaaaaatcg
     4501 aacctacatc atttaataaa tttataacta taggtgtaaa cattattaaa ataaaacccc
     4561 tctactacta tatgtaacga agggacatga tttcaaaata aaataccttt tttataaatt
     4621 t
//
LOCUS       DALAAR010000131         4573 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000131 DALAAR010000000
VERSION     DALAAR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.136"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(301..945)
                     /locus_tag="O2458_002657"
     CDS             complement(301..945)
                     /locus_tag="O2458_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCZ3888491.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1306..2103
                     /locus_tag="O2458_002658"
     CDS             1306..2103
                     /locus_tag="O2458_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ3888492.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(2691..3698)
                     /locus_tag="O2458_002659"
     CDS             complement(2691..3698)
                     /locus_tag="O2458_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCZ3888493.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(3714..4331)
                     /locus_tag="O2458_002660"
     CDS             complement(3714..4331)
                     /locus_tag="O2458_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCZ3888494.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT         1641 a          770 c          622 g         1540 t
ORIGIN      
        1 atgtttcgat tttaaaatta atacgtagct atactttact tttctaataa aagtgtaatt
       61 agaaaggcaa gtacacctgt ttaaagttta tcttctaaga tttgtttact ttagaactat
      121 agcagttaat catttcaatg attaataata tctttacaat taaggtaata tatttttatt
      181 gctttatcag taaaatctca gtctaagttt ctaacttatg acaatcgctc cagtttaagg
      241 ttgatattca ttattgatta ttccaaaact ttatacaaat ttctttatta atttaactgt
      301 ttatcttctg aacatattag caaggtaaag tatatcctta ataatattca acaaattaat
      361 aaatagatta aatcccattt ctcttggtga atagtcacct ctttttaaac gattaaaatc
      421 atacaaagta taaagtagaa ataacaacag actaacgacg gtaataatag tgtaaaaaat
      481 aggattttga agaaaaatac caattagact cgcaataatt aatgttatta atgtaacgaa
      541 taaatatttg cctatactcg atgcatctcc aacgaagaaa taaccaataa taccaaatgc
      601 tataaatgca aaaattgcta atgcgatatt tttatagaaa atatctggtc ctaaattttg
      661 taaatacgtg gtaaacgttg cgtatgacaa taagccaatc actacagcgt aaatatgtga
      721 aataattaaa ccgaaccgtc tcgctctttc aaaaacaagt gttgctaaaa taataatcag
      781 taatccaaat gacaagggtt gtcgccaact taatggtaaa aactgaccta gatagcatcc
      841 tatgccaaat ataatccaat aatacataaa ataaagccac actttactta ttgattgagc
      901 atgctggttt tgatgataat atgaatgatt tgtattttgc gacaaaataa ttcctccttc
      961 taccgtcgtt ttatgcgtca aattttacag taaattttgc ttcatcaaaa gaaataacct
     1021 ttaacaagta tagtaatttt acattttaca atgttacaaa atataatttt ttataattag
     1081 ttaaaatcac taaaacgctt ttatacacta tcaaatcagc atttataaaa atatgaaccg
     1141 atatcctaaa atgttaataa tattacaaga taataacaaa ccacacaaag ctacttattt
     1201 ttgataatat ggaaatcgta atataaaacg aaaacttaat ttactatata aattgtctta
     1261 ataattttta aaagtagtaa aacataattt taaggaggag tccctttgaa aaaattagca
     1321 tttgcaataa cagcaacatc tggtgcagct gcatttttaa cgcatcatga tgcacaagct
     1381 tctacacaac atacagtaca atctggtgaa tcattatgga gtattgctca aaaatacaac
     1441 acttcagtag agagtattaa acaaaataac caattagata acaacttggt attccctggt
     1501 caagttatct cagtaggtgg aagtgatgca caaaatacgt caaacacttc tccacaagct
     1561 ggttcagcat catctcatac tgtacaagct ggtgaatcat taaatatcat tgctagcaga
     1621 tatggtgttt cagttgatca attaatggca gccaataact tacgtggtta tttaattatg
     1681 cctaaccaaa cattacaaat tcctaatggt ggatcaggtg gtacaacacc aacagctaca
     1741 acaggtagca atggcaatgc atcatctttt aatcaccaaa atttatacac tgctggtcaa
     1801 tgtacatggt acgtatttga ccgtcgtgct caagctggta gtccaattag cacatattgg
     1861 tcagacgcta agtattgggc tggtaacgca gctaatgatg gttaccaagt aaacaacaca
     1921 ccatcagttg gttcaattat gcaaagcaca cctggtccat atggtcatgt tgcttatgtt
     1981 gaacgtgtca atggtgatgg tagtatcttg atttctgaaa tgaattacac atatggtcca
     2041 tacaatatga actaccgtac aattccagct tcagaagttt ctagctatgc attcatccat
     2101 taattaaata aattgtactg atatatacta gcaattcaca tcatgtgaga ttgctagttt
     2161 tttatttttg aaaaaaattt tcattttggt acaaaaaatt atctcaccct tccctatcat
     2221 acatatttat attttgtatg aatggtagtt aggtaaaaat taacaaccta cctatttgat
     2281 taaattaaat gcataatata ttaaaaatca aaataatgca actaagttga ctatataatg
     2341 atttaatttg ttataccagt attttacgct ttttcgtcta catatacaaa tttatattaa
     2401 ataaagccca atacaattta ggttaattaa acaagttgat aactatttaa ttattccttc
     2461 attgaagaat ataaactatt aaatcattat tttgctctta catatatttt aatgacctaa
     2521 ctgattatgt tccatggaat acatttataa tatagcctcc taattaaaat gctttgtctt
     2581 ggtcattcta cgtaaattct ataaaatatg ttatctactt acataaaaaa ctgaacttca
     2641 ataccaccat atgtttgtga tactgaagtt cagtttagtt ttattttcaa ttagaaaaat
     2701 aagttaagta tatagaatag taaacctgct aacaatgctg aaataggtaa tgtaatcacc
     2761 catgtaatga tcattcgttg cgcagtgctc cattttacac ctttagctcg gttagaagca
     2821 ccaacaccta agattgatga tgacacaacg tgagttgttg ataatgggaa atgtagcgat
     2881 gatgcaacaa aaattgttaa tgcagatgat aaatcggccg cagcaccatt tgctggacgt
     2941 attttcataa tattaccacc tacagttttg ataattttcc agccaccaat tgcagtacca
     3001 agccccattg ctgtcgcaca ggcaaatttt acccataact gtggttcaac actgccatca
     3061 ttctgtacat tagcgacaat caatgccaac gtaataatac ccattgattt ttgcgcatca
     3121 ttcgtaccgt gagagaatga ttgtaacgct gctgtgaaaa tttggaaaaa tctaaagtta
     3181 cgattcgctc ttgttaaatt tgcattttta aagataactt taaaaattga atacatcaag
     3241 aaaccaacac aaaatgcgat aatcggtgaa acgattaata caataataat ttttgtgaaa
     3301 ccttggtaat gtaacactcc aaatgagcct tcagatgcga ttgctgcacc cgcaattgaa
     3361 cctataagtg catgtgaaga cgaacttgga attccgtaaa accaagtagc taaattccaa
     3421 ataatagccg caagtattgc agctaacaca acaactaatc cattttccaa tttaaatgga
     3481 tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa atgttaaagc acctataaag
     3541 ttcatcactg ctgccattaa aattgccgtt ttaggcgtta acgctctagt agatacagca
     3601 gtagctactg cattggctgt atcatggaat ccattgataa agtcaaatat cagcgagaaa
     3661 ataactacag ctatagtgac gatgattata tatgacataa atatatactc cccttagcta
     3721 tttttcataa taatagtttc aaaattattt gctacgattt gacatttatc agcgatttct
     3781 tccatgcttt cataaatatc ttttatttta attaaagtga ttggatctgt ttcgctattg
     3841 aaaatatgtt taattgactg tcttaaaata ccatcacagt ttgtttcaaa ttctttaata
     3901 ttaattgaat gaatacgcat atgtgataat tttttatcga ctaataagcc gacagcaagt
     3961 ttcatttctg caactgcttt ttgaatgtta tcaacaaact cagccatata ttcatctgtg
     4021 tattcgattg aatacatttc aaacatagct gccgtttctt caattgcatc taaaacatca
     4081 tcaattgcat cacataatga taaaatatct tcacgttcaa ttggtgtgat aaatgtttga
     4141 tttaaatcag taattacttg atgtactaat tcgtcaccat gtgactcata agttttaatg
     4201 ttgtctgagt atgcttttaa atctaaatgt gtattgaaat ccattttacc gaattcaata
     4261 gcagcacgat ccagattgaa aaccatctct tctaattgaa ccataaactt atcttttttc
     4321 ttactaaaca tttaaaatcc tccatttaag cgattgtcac caatcacatt cagttataat
     4381 ttgtttcaaa ttaagacaag tgaatttaca aactaatgat acaaatttgt tattatcaat
     4441 cgtcagtata attttagtgt actgatatta atttcaaaaa tgcctcacag taaacaattt
     4501 actgtatttg cccttatatg aaaatagcac aaaatttaca atcccacaat tcaatttaca
     4561 aatttttaac aat
//
LOCUS       DALAAR010000132         4485 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000132 DALAAR010000000
VERSION     DALAAR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.137"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(2..157)
                     /locus_tag="O2458_002661"
     CDS             complement(2..157)
                     /locus_tag="O2458_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888495.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(671..1642)
                     /gene="nrdF"
                     /locus_tag="O2458_002662"
     CDS             complement(671..1642)
                     /gene="nrdF"
                     /locus_tag="O2458_002662"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCZ3888496.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(1760..3865)
                     /gene="nrdE"
                     /locus_tag="O2458_002663"
     CDS             complement(1760..3865)
                     /gene="nrdE"
                     /locus_tag="O2458_002663"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCZ3888497.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(3828..4226)
                     /gene="nrdI"
                     /locus_tag="O2458_002664"
     CDS             complement(3828..4226)
                     /gene="nrdI"
                     /locus_tag="O2458_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCZ3888498.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
BASE COUNT         1381 a          804 c          673 g         1627 t
ORIGIN      
        1 atcattgata atatttctca ttaatagtag cgttttttga ccataatttc aatagccttt
       61 caaataattt agtgtcaatt ttattgatat ttgcaattaa actgaaattt aatttttcgg
      121 aatgtgtatt ttgtttactt aaagtaaaat agaacactta agattttgat gtctgggaat
      181 agtggaaatg ataaaaacta ctaatgattg attaagtagt ggttcttaac attagcctca
      241 gctaatgtgt acttaaaaat aggaatacat gagtaaaact catgtggaag tgataatttc
      301 taaaggaaaa agtttttctt tatgttgggg ccccgcccga acttgcacaa tattgttagc
      361 tgactttccg cctgctttta tgttggggcc ccgccaactt gcacaatatt gttagctgac
      421 tttccgcctg cttctatgtt ggggccccgc caacttgcac aatattgtta gctgactttc
      481 cgcctgcttc tgtgttgggg ccccgccaac tactgccaat ataaatagca ttgtacatct
      541 tagagcattg atttcttact cagactcata atgagttaga tacacataaa aaaagacctt
      601 caacatgtag gagacaagac gaaacgaatc gctatttccc tttacatgtg aaggtctttt
      661 aatttaattg ttatttgttg tcaaatacaa agtcatcatc ttgtaatgct tctacgttta
      721 atgctaatac ataaccatca ccttttactg agaagaagtc atggttttta gttgttgtat
      781 ctaaggcatt ctcaatgatt gggttaaatt cacgttcctc aaaataaggt tcaaagccta
      841 agtttgaaag tgctttgttt ccattatatt taacatagtt tagcacatct tcagtgattc
      901 caagatcatc gtataacatt tttgtgtatg actcttcatt taaatacaag tcatttagca
      961 atttatacat ttcttgatct gctttttgtt tctcactttc agatagttca tttcgtaaat
     1021 gctgtgcatc taaaccggta aatacaccat gaatagattc atctaaaaga attttacgaa
     1081 tgatttcacc tgatgtcgtc attttccctt gaccagcaag atatagtgga taatagaaac
     1141 ctgagaagaa taagaatgtt tctaaaaata cactcgtaac tctggccata tattggtcgt
     1201 atatcgaagc ttctttaccc caaagtttgt gataattagc aacaatttta tcagatttat
     1261 attttaaatg gggttcctct aaaacccatt catctaatag gtagtttgtt tcactagatg
     1321 gtaatagtgt tgtgaaaata tgtgaatagc ttttcgcgtg tatttgctcc atcatcgcca
     1381 taaatgaata aactgctttt ttccttaagt cagtcgtatg tagcataact aaaggcatgc
     1441 catcatctgc ttgatgtgta tctaagcctg ttaaaccagc taatgctttt ttaaatgtgt
     1501 cttgttcagc ttcagataaa gtcttccaac ttgcaatgtc ttttgatact ttaaattctg
     1561 tttcaaccca catttgagat atattttgtc tccaaaacat attcgtcata tcttcttgtg
     1621 tgttccaatt aacagctatc atgaatataa tttcctcctg taatgttact caataattga
     1681 caaatacaga catagcagtc taatcatctg ctaattcaga gcagatgatt agctgtttgt
     1741 cattttcaac atttaattgt tagatagaac aacttgtaca ttcttctaca cttaataatt
     1801 tatttctagt atagtaaagt gattttaatc ctttatagtg cgcatataca tataatcttg
     1861 ctaactcacg tgtagaaatt tcagaattaa cataaaggat cgttgagata ccttggtcaa
     1921 tatgcgtttg aattgtcgca attaaatcga ttaatttcat ctgatcagta ttgaatgctg
     1981 atttgtagta ccacattgtt tgtggtgata agaatggcat agggtaaaat gtttccgcat
     2041 taccataagt acgacgttca atttggtcaa cgattggcat tacagaactt gttgcatttt
     2101 gaacataaga aatactttgt gttggagcaa ttgctaatct atatgcatga tataaaccat
     2161 attgttcaac atcttgttgt agtttcttcc aatcctcaga agtaggaata gccataccat
     2221 cgaataattc acgtactttt tcgaattgag gttcaaattc ttgagttgta tagaactcga
     2281 aatatttgcc attagcataa tcagactttt caaagtcttg atatttgata ccacgctctt
     2341 tagcgatttc cattgaacgt tcgattgagt agaaattcat catcataaag aagatatttg
     2401 caaaatcttt tgcttcttct gactcataac caattttatt ttttgctagg taaccgtgta
     2461 aattcatcac accaagacca actgaatgta attcactgtt agcttttcta actcctggtg
     2521 cattttgaat atttgctaca tcactcacaa cagttaatgc gtccatacca gagtgaactg
     2581 aatctctgaa ttttccgctt tccattacat taacaatatt taatgagccc aagttacaag
     2641 aaatatcacg tttaatttcg tcttcaatac catagtcatt aataattgaa gtttcttgta
     2701 gttggaaaat ttccgtacat aagttactca ttttaatttg tccaatgttt gaattcggat
     2761 gcactctgtt agcattatct ttaaacatta aatatggata acctgattgt aattgtgttt
     2821 gcgcaattaa attcaacatt tcacgcgcat tctttttctt tttctcaaca tttgggtttg
     2881 caaccatgtc atcataatat ttttctaaat cgatatcgtc taatgtcaca ccatattctt
     2941 ctttaactgt atgaggtgca aacatataaa agtccttacc ttctttagct aaatcgaaga
     3001 atttagatgg aacaattaaa ccagttgata ttgtagataa acgtaaatct tcatccgcat
     3061 ttactttttt agtatctaaa aattcttcta catcataatg gaagatattt aagtacacag
     3121 caccagcacc agggcgttga ccaagttgat ctgcatagct aaagccacct tcaagtgact
     3181 tagcaatagg taaaacgcct ttcgctacgc ctttaattcc tttaattgct tcaccacgtg
     3241 cacgcaattt agataagtta attgcaacgc cgcccccaat tttacttaat tgttttgcag
     3301 ttgaatcaat aaagttaatt gaatttaagc tgtcatccac ttctaataag aaacatgaca
     3361 ctagctcacc acgacgcgca cggcctgcgt ttaaaaatgt tggtgtcgct ggttgatatc
     3421 tttgttcaac catagcagaa ataaattgtt tagcttgtgc tttattacca tttgctaggt
     3481 ataaagcaac aatggcaacg tgttgattat agtcttctaa gtattgactt ttatcatttg
     3541 ttttcaaagc gtaatctttg aaaaatttac tagctgacat ataacttgca aaattaaacg
     3601 ggattgattt tgcataatca gtgatttcaa ttagatccgc ttcactataa atatcaaaca
     3661 cattgaaata aaaatcgttg tctactaaat aacgtaaacg ctcgatttca gtgtcgaaga
     3721 agattgtttt gtcttttact tcttctaaat aagctactaa agcttcttgg tctttttcta
     3781 aactaaagaa tccatcttct cttcgtttag tgacctcatt atttaattca atatgattgt
     3841 actttttctc ttccatggtt ttcattaaaa ttacccacct tgttcttaaa ttcaataacg
     3901 tctttgtttt ttccatgtaa ctcaaacttc attaataaag ggacattata ctcttctgat
     3961 atcgtgcgac ccgcttttgc gaaatttagt ccccaatttc gattaccgct agctgccaca
     4021 cctctgatgt attgatgatt aacttctaaa aaagattgaa cgggttctgg tacttctcca
     4081 aatccaatag tgccagtaac gataataaac ggttcatgaa ctggttccat acaattttct
     4141 gctgtaatct caagcgtatt ttcaagttct gttctcttaa taaaacgacg gacatttcca
     4201 gtaaatgaaa aatatattat tttcattgga tcaccttctt tcttcaaccc ttttaaataa
     4261 agtagtcatt ccaattaaaa aatgctttaa caacatggtt actatggtta tctcagcatt
     4321 gtattaacta taatgaagct acttattttc tcaaatgtaa accaattgca tagatttata
     4381 tttgagaaaa tagtagctta tatgggttga atgtataaaa atcactatat gtagtgttta
     4441 catcattgga tatcttccat ttgctcagta cattttctaa aaata
//
LOCUS       DALAAR010000133         4388 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000133 DALAAR010000000
VERSION     DALAAR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.138"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            215..523
                     /gene="spn"
                     /locus_tag="O2458_002665"
     CDS             215..523
                     /gene="spn"
                     /locus_tag="O2458_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCZ3888499.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(626..2134)
                     /locus_tag="O2458_002666"
     CDS             complement(626..2134)
                     /locus_tag="O2458_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCZ3888500.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2156..2833)
                     /locus_tag="O2458_002667"
     CDS             complement(2156..2833)
                     /locus_tag="O2458_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCZ3888501.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKENFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLK
                     ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNI
                     EKIEVNL"
     gene            complement(3216..>4388)
                     /locus_tag="O2458_002668"
     CDS             complement(3216..>4388)
                     /locus_tag="O2458_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ3888502.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
BASE COUNT         1324 a          769 c          532 g         1763 t
ORIGIN      
        1 atttttttat tattgtttca tttgatttaa accctttttg aaatcaaggt tttattagga
       61 aaaatcctca agcatcgtat taaagattaa ttgcatttaa acaatatttg tcactttaac
      121 tatttattaa aaattattaa atcgacttaa taaacgtaat tttcttaata ttcagtgtta
      181 tgatggagtt acaattttta gaggagtgat agatatgaaa tttaaaaagg ttttagtagc
      241 tacggcaatg gtaggtgttt tagcaactgg cgttgttgga tatggtaatc aagcagatgc
      301 gaaagtttat tctcaaaatg gactcgtact acatgatgat gcaaacttct tagaacatga
      361 attaagttat attgatgtgt tattagataa gaatgctgac caagctacaa aagacaactt
      421 aagatcatac ttcgccgata aaggactgca ttcaatcaaa gacattatca acaaagctaa
      481 acaagacggc tttgatgttt caaaatatga acacgtaaaa taaattcaat aatgctttgt
      541 aacaaagcac aaaaaacagc tcaatgacgt agaagtcagt gattaactca ctaatttcat
      601 tacatcatta agctgtttta tttaattatt tttctaattt tggtgcctta cgttcaactt
      661 tcttttcacc taatggataa aaccaggtaa tcgggaatcc gttagtatcg tagccgacaa
      721 ttttatcacc atgtaattta tgatatgcag gtgtgttttc cattctttca tcaaactcac
      781 gatctgcttt ttcttcagga gattcataaa tatctatgtt tttagaaaca aaatttccac
      841 ttaataattc acctgtaata ggatcaacta ctgtgaagtt tagtgtttct ccattttgta
      901 atctaaatcc agcatgatct acatcaaagc tgaattcacc tttttcatcg acttgcaaat
      961 cagggaattc ttgaccttca cctagttcag agtctacctt cacaactgca tttgcgaatt
     1021 tagtcttacc tttaattact ttagtacctt ctttatcaag aataccttta cctttttctg
     1081 tatgatataa gcttccgtaa atttcatcta catgtaaatc ttctttaaca tctttcaata
     1141 cttttacttt ttccacttta gtatggtcat atttagtttc tgctttatca atatcttcta
     1201 aactcgttac cttagttttg aaaattaatg acttcaatgc ctcatcttcg tcatcaggtg
     1261 caaatgttaa tgagatctca tcatttttct ttaactctaa atcatcgaaa cgtttcgttt
     1321 tgaagtcaaa attaaagtat cctttgtcat caataggcat ccagattcct tctgatccag
     1381 atttcgcttc ttctttattt ggaccaccat tagcatttgt ctcaaagtta ataaatttat
     1441 tattaataga tagagcaact ttacctttta cagaggtatg gcctttaata atacctgaac
     1501 cttcagtaat aggttcgata aaaacttggt gatgtccatc tttttctttt agctgttctt
     1561 tcggtatttc atacgctttt tcatatcgcg gtgttgtata tgtagcttta gcttctgtac
     1621 tttcttcctc agcaccaact tcttcagttg aagtttcttc tacttcttca tcttcttcat
     1681 cgtcacctaa atagggatct gatgaagaca cttcaatttc ttgattatga acaattttgc
     1741 gaccatttaa gtcatactta aaattcccat ctttatcaga cataacaaag ctgctgtcag
     1801 aatcttcaac tgagctcaca tcttttttat caatagttaa tagtataaat tgatttggta
     1861 aagttgttcc ttgtatagaa gtatcacctg gtttaatatc attcaatttg acaaaattag
     1921 ccggtacttt tgatgtttca ttagtattat tttgaggctg aacagcttga tttacattat
     1981 tctgtttttc ctgattgtct acatttatat tagagattaa agcgttttga ttttcagatg
     2041 cttctgcttg tccgtttgtc ataatatata acattgtaat tgcaacagat actaaaccga
     2101 ctttcatttt acgtaactta aaattttccc tcatgatatg ctccctcgaa taattttata
     2161 aattcacttc aattttttct atatttctgc catcaataac atcacccata cggtgtgttt
     2221 gtaacttttt attcaattca aatgtgtaga acccaccatc tttcatagta attcgaattt
     2281 tactgtcttt aggttctgtc ttataaaggt tatgtttatc aattaaatgc tttcttaatt
     2341 taaaatcaag ttcttttaat gaaatttctt ctttattaat tgtatatgta gatgatgttg
     2401 acgttgatgt aacactatcg atgtttttag taattattaa atttacatct tttactttat
     2461 caatataatt tgaaccgttt gttttagtaa taccaccaat tgaagctgta ttaccagatc
     2521 tatcagaatt ttctcttaca acaaatatat ctacattaga aatatcttca ccaaaatttt
     2581 ccttttcatt cccagttaaa gttacatcta taagttgata attaggagta aacgtcactt
     2641 ttccttcctc tttatatcct gactgatttg tatactcaaa gaacggtcta ttataatatt
     2701 cactcaagtc ttgagtagct tgtgatctaa cctctaatgt acttgctttt actggctgag
     2761 cagtagttgt aatcatccct gttgttaaaa tccctagtgc taaacttgct ttagcaatat
     2821 tttttaattt cataattcta tgctcccaat ttttagtcta tttgatttat tctattacgc
     2881 aatacgaaca atcctcattc attataatga gcaatggtcg tgcttcacat taaacttact
     2941 ttaactaaaa attaatcatt attaagtaat ttaataaata gttaactaat atcattcaat
     3001 tcctatcgat ctactctctt ttatttacga ataacacttt atctcaactt aatctttatt
     3061 taactactta ttctgtacac aatttcgaca caaaaaagac actgcgcttt cacagtgcct
     3121 aaatttaatg ttcgttttta attcacttta cttttacaac ttttatgtta aattatacaa
     3181 attttattct tatgcaatct atgtatttat caaagttata agaacatttt ttgtaaaaag
     3241 gattgtttct ctttttctaa tatttcaatt ttcattttct gtttacttat caatatatcc
     3301 atttttttaa agaaatcacc tatcttttct tgttcctcca atacaggtat atctatattt
     3361 atatttttta attgtttata ttttaagttc catgtatctg atgttaatcc ttgtgaatta
     3421 attttaaatt tatgaatcat tctatgtgtt ttaaacttat atccaataaa taatgagcta
     3481 gtattttgtg ttggataaag cacagtatat gcagggctaa caatcccatt ataatttgat
     3541 ttaccactag ccccttgcca cattctcata gaattatatg caatatcatt tttcctaact
     3601 actttataat tacttttatc tttacttgaa ttatcttttc tatccaattc actaaatttt
     3661 ataatgccac tatttatagt tactgaaagc atttgccctt tgtcagaacg ttcgtttctc
     3721 tcttttaaat atttttctat tttgctattt tcccaatctg gataatcttc accattctca
     3781 tctttgaatc gcagttcctg tgagaaaatt ttctgcatat agcctttttt ctgttgttga
     3841 agtaattcaa gcttttgttc ttctaattca atttgtcggt cgagtttgct gaagaacttg
     3901 cctatttttt gctgttcttc taaacttgga tatttaatta gaatagtaaa aaaatcattc
     3961 acagaaacat ttaataatcc gtgatttctt gcaccctcaa ctgcaattcc agaaacttct
     4021 ctataccagt gtgtcgaatc aaaatatgct tccatgaagt ctttagacat ttcactttta
     4081 atagaaaaac aaatatacaa agaggacaat acaccactat catatctagt taatctttta
     4141 atagccccta atgggtatcc attagaataa cttttgttat acgcgaattc tccattcttt
     4201 attagtgtat aattttctag atttttcgac gaaactgatt tactaaaata ttctgtttga
     4261 tcaattaaac ctaactgtcc ggatattgtt aaaggctttt tcgattctaa gtttttattt
     4321 ttcctaatta ctctatctgt aagatcccct aactgcttct cttcccattc gccttcaaac
     4381 cctgggaa
//
LOCUS       DALAAR010000134         4387 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000134 DALAAR010000000
VERSION     DALAAR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.139"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            1092..1961
                     /locus_tag="O2458_002669"
     CDS             1092..1961
                     /locus_tag="O2458_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCZ3888503.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(2032..2394)
                     /gene="mscL"
                     /locus_tag="O2458_002670"
     CDS             complement(2032..2394)
                     /gene="mscL"
                     /locus_tag="O2458_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCZ3888504.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            2598..4244
                     /locus_tag="O2458_002671"
     CDS             2598..4244
                     /locus_tag="O2458_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ3888505.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         1585 a          597 c          769 g         1436 t
ORIGIN      
        1 agaaaaaaat gttagaagaa aatggagatt taattgaaag taaaatctct tttattgata
       61 aaactagagt attatttaac gatattaata agtatcaaca aagttatctc aatattgaac
      121 gcttgagaac tgagggtgaa caattaggtg atgaattaaa tgatctaatt aaaggtttag
      181 aaacggtcga agattcaata ggtaataacc aaagtgatta cgagaaaatt atcgaactaa
      241 ataatacgat aacgaacata aataatgaaa ttaatataat taaagaaaat gaaaaagcta
      301 aagctgaatt agataaacta ttaggtagta agcaagagtt agagaatcaa attaatgaag
      361 aaacatctat attgaagaat ctcgaaataa aattagatcg ctacgataaa acaaaattgg
      421 acttaaatga taaagaaagc tttataagtg aaattaaatc tgctgtaaat attggagatc
      481 aatgtccgat atgtggtaat gaaattcagg atttagggca tcatattgat tttgacagta
      541 ttgctaaacg tcaaaatgaa attaaagaaa ttgaagcaaa tatccacgct ataaaatcga
      601 atattgctgt gcataattct gaaattaaat ttgttaatga aaaaatatcg aatattaata
      661 ttaaaacgca aagtgatttt tcacttgaag tattgaataa gcgtctgcta gaaaatgaaa
      721 atgcattgaa taatcaaaga gatcttaata aatttataga acaaatgaaa gaagaaaaag
      781 ataatctaac gttgcaaatt cataataaac aattgcgtct aaataaaaat gaatctgagt
      841 tgaaattatg tcgagatctc atcactgaat ttgaaacact ctcgaaatat aataatatca
      901 ctaattttga ggtggattat aagaagtatg ttcaagatgt gaatcagcat caagaactct
      961 caaaggagat tgaagataag ttaatgcaat tgtctcaaag aaagttaatt gagcaaaata
     1021 atctaaatca ttatgaaaat caactagaaa cttacaataa tgacttagaa ttgaatgaac
     1081 agtctattga aatggaaatg tcgaggctga atttaactga tgacaatgat atagatgaaa
     1141 taatagcctg gaggggcgag caagaggaat tagagcagaa aagggatact tataaaaaac
     1201 gttatcatga atttgaaatg gaaatagcta ggttagaatc attaactaag gataaagagt
     1261 tattggactc tgataaatta aaagatgaat atgagctaaa aaaaggaaag atgaatacac
     1321 tgatagatga atactctgct gttcattatc aatgtcaaaa taatattaat aaaacacaat
     1381 ctatagtttc gcatattaat tacttaaatc aagaattaaa ggatcaacaa gaaatatttc
     1441 aattggctga aattgtcagt ggtaagaata acaaaaatct tacattggaa aactttgtct
     1501 taatttacta tttagatcaa attattgctc aagcaaatct gagattagca acaatgtcag
     1561 ataatcgata ccaactaatt aggcgagaag cggtttctca tggtcttagt ggcctagaaa
     1621 ttgatgtatt tgatttgcat tcaaataagt ctagacatat tagctcgtta tcaggtggag
     1681 aaactttcca atcgtcgctt gcattagctt tagggttaag cgaaattgta cagcagcaat
     1741 caggaggtat ttcactagaa tcaatattta ttgatgaagg attcggtaca ttagatcaag
     1801 aaacgcttga aacagcgtta gacactttat taaatcttaa atcaactggt agaatggttg
     1861 ggattatttc acatgtgagc gaattgaaaa atagaatacc tttagtttta gaagtgaaat
     1921 cagatcaata tcagagttca acaagattca aaagaaatta aaacataaaa aatagagatg
     1981 gtactttgga atgtatagaa caaccttagc ccatctcttt ttatttaaaa attatttttt
     2041 ctcacgtaat aaatctctga tttcagttaa taacacaaca ttttcttcca caactgcttc
     2101 ttcttcggct tcttctttct tcattaatgt atttgcaatc ttaacaaaga tgaataaagc
     2161 aaacgcgatg ataataaagt cgataacaga ttggataaat aaaccgtatt taatacccca
     2221 gaatgaccat tctttagcaa aatcaactga tccgaaaatt ttaccaatta atggcataat
     2281 gatattttct actaatgaag atataatctt gttgaaagct gcacccatca caacagcaat
     2341 tgctaaatct aagacgttac cttttaaggc gaactctttg aattctttta acattacact
     2401 caacctctct tttttaaatg taacctcaat tatacaacaa ataaaaaaat tagaaaatag
     2461 attattcaag aaaatttgat tatcgcaaag acctttgtta tcctataaag gtgcgtgagt
     2521 tattgaataa aatgtttaga taatattttt attctactac ataaaattaa atatagaatt
     2581 gaagggagta attaattatg aattcttctt caccagagaa tccaaatgga aagaagtatt
     2641 caccagtctt catctatagt gcaattgttg ttgctatagt cgtattactt ggtgcatttt
     2701 tacctgaaca attcaactat gttaccaata atattaaaat gtggattaca gaaaagttag
     2761 gttggtatta tcttattctt actacgatta tcgtgttctt ctgtatattc cttattttta
     2821 gtcctattgg aaaacttaaa ctaggtaaac caaatgacaa acctgagttt aatacaattt
     2881 catggtttgc tatgttgttt agtgctggta tggggatagg tttggtgttt tatggtgcag
     2941 ctgaaccgat ggcgcacttt gctacgccac ctacagcaga tcccaaaact actgaagctt
     3001 atactgaagc tctacgttca acatttttcc attggggatt ccatgcttgg gctgtttatg
     3061 gtgttgttgc gttagcgttg gcatattcgc aattccgtaa aggtgaacca ggtttattat
     3121 ctagaacttt acgtcctctt ttaggtgata aagtagaagg tcctattggg atttttattg
     3181 acgttttatc tgtatttgcg acaatcgttg gggtagccgt ttcgttaggt atgggtgctc
     3241 tacaaattaa tggtggttta cattacttgt tcaatgttcc aaacaatacg tttgtacaag
     3301 cgattatcat cattgttgtt actatcttat ttatagcaag tgcatggtct ggattaagta
     3361 aaggtattca atacttaagt aacttgaaca ttggtttagg tactatttta atggtagctg
     3421 ctttaattgt tggaccaact gttcttattt taaatatgtt aactagctct acgggtagtt
     3481 tactaaacac attcttgttt aatagttttg atacagcagc tttaaatcct caaaaacgtg
     3541 aatggatgtc ttcatggaca ctttattact ggggttggtg gttaagttgg agtccattcg
     3601 ttggagtgtt tattgcacga gtttcaaaag gacgttcaat tagagagttc atttctggtg
     3661 tcttgctagt tccagcaatt gttagttttg tttggtttag tgtctttggt gtattaggca
     3721 tcgagacagg taagaaacac aaagaaattt ttgatatgac tcctgaaaca cagctatttg
     3781 gagtgtttaa tcatgtgcca tttggcattg ttttatcgtt gattgcatta ttattaattg
     3841 catcattctt tattacatct gctgactcag caacatttgt attaggaatg caaacaacat
     3901 ttggttcatt aaatccatct agtatggtaa aagttgtttg gggaatttca caggccttaa
     3961 tagcatttgt acttttatta gctggtggcg gtaacggcgc tgaagcttta aatgcgattc
     4021 aaagtgctgc aattataagt gcattcccat tctcctttgt cgtcatactc atgatggtaa
     4081 gtttctacaa ggatgcgaac caggaacgta aattcctagg tttaacattg actccgaata
     4141 aacatcgctt acaagaatat atcaagagtc aacaagaaga ttatgaatct gacattcttg
     4201 aaaagcgtca gtcacgtaga aatatagaga aaaaagataa ctaataaagt ttagttaagt
     4261 attttaataa caagtagtat gtcattctag tagctagaac agatatacta cttgtttgtt
     4321 tttgtggaaa attgagtata ttcaaaagga taagaacgag aatttcgtta ttaaactacg
     4381 aattctc
//
LOCUS       DALAAR010000135         4344 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000135 DALAAR010000000
VERSION     DALAAR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.140"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            69..530
                     /gene="tsaE"
                     /locus_tag="O2458_002672"
     CDS             69..530
                     /gene="tsaE"
                     /locus_tag="O2458_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCZ3888506.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            511..1173
                     /gene="tsaB"
                     /locus_tag="O2458_002673"
     CDS             511..1173
                     /gene="tsaB"
                     /locus_tag="O2458_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCZ3888507.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1146..1610
                     /gene="rimI"
                     /locus_tag="O2458_002674"
     CDS             1146..1610
                     /gene="rimI"
                     /locus_tag="O2458_002674"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCZ3888508.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1603..2628
                     /gene="tsaD"
                     /locus_tag="O2458_002675"
     CDS             1603..2628
                     /gene="tsaD"
                     /locus_tag="O2458_002675"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCZ3888509.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2730..4340)
                     /locus_tag="O2458_002676"
     CDS             complement(2730..4340)
                     /locus_tag="O2458_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCZ3888510.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
BASE COUNT         1481 a          619 c          815 g         1429 t
ORIGIN      
        1 agttagatac gattgaacaa tggataatca tttttatgaa tgaaaagcat aatataggag
       61 aatcaacatt gataaagata aataatttag atgaaatgaa tcaatttgct atatttttag
      121 ttgagcaatt gaaaagtggt gatttgattt tacttaacgg agatttagga gcaggtaaaa
      181 caacgttaac gcaatttata ggaaaagctc ttggtgtaag acgtacgatt aattccccga
      241 catttaacat cattaaatca tataggggta aaaatttaaa attgcatcat atggattgtt
      301 atcgcttaga agattctgat gaagatttag gatttgatga atttttcgaa gatcaggcaa
      361 ttactgttat tgaatggagt caatttataa aagatttact tccagcgacg catttatcta
      421 ttaacatttc aacaatatct gaaaatacaa gacaaattga gttgttcgcg caaggagaac
      481 attatgaaca aattaaggag gcaattatcc atgaattcgc tgctcattga tacatcgaat
      541 caaccattat cagtagcatt gatgcaaaac gatgaagttt tagctgaaat aactacggat
      601 tctaaacaaa atcattcagt gcaattaatg cctgcgatta gtcagctatt tgagcagagc
      661 aaaatagcta aacagcaatt agatgcgatt attgttgctg aaggtcctgg ttcatatact
      721 ggcttacgta tcggtgttac tgttgctaaa acattagcgt atgcgttaga tgttaaatta
      781 tatggtgttt catcattaaa ggcattggca gcaacaatcg atcatactga taaattattg
      841 gttcctgtgt ttgatgcaag acgacaagca gtttatacag gcatattcca gtggcaaaat
      901 ggtcaattag tgactatttt agaagatcaa tatatgtcga tagaggcatt gcgagctttt
      961 ttagaagatt taaaccaacc atttatttat attgggaaag atactgtaaa attacaagat
     1021 gaattacaag gtgaagtgat tgcgcagcta ccaaatgcat cagtcatgta tcaattgcta
     1081 gataaacctt cagacgtaca tacatttact cctaaatacc ataaattagc tgaggcggaa
     1141 cgaaattgga tcaacagtca aaagaacaat taaacattag agagatgact aaagaagatg
     1201 tgccacaagt ctttgatctt gagcgtcgga gtttcaatga cagttcttgg acaattgacg
     1261 cattttatca tgaaatagag caaaataact ttgcgaaata ttttgtctta gaatttgaac
     1321 aacaaattat tggttattta ggtttatgga ttgttatcga tcaagctcaa attacaacag
     1381 ttgcaatcga tgataattat agaggctatg gtttaggtca aatgctttta aaatacggaa
     1441 taaactatgc aagtcacact tgtgatgtga tgagtttaga agtaagagtt aataataaag
     1501 tggcacaaca tgtatatgaa aatttaggat ttcaatatgg tggtaaacgt aaaaattatt
     1561 atggtgaagg tgaggacgca atggttatgt gggtgaattt aaatgactaa agatatatta
     1621 atactagctg ttgaaacaag ttgtgatgaa acaagcgtta gtgttataaa aaatggcaga
     1681 gatattttat caaatacagt tttaagtcag attgaaagtc ataaacgatt tggcggtgtc
     1741 gttcccgaag tggcaagtag acatcacgtt gaaggtataa cagcaacaat aaacgaggct
     1801 ctaggggatg ccgatgtatc aatagaagat attgatgcca tagcggttac agaaggccct
     1861 ggactaattg gtgcgttact aataggtgtt aatgcagcca aagcattggc atttgcttac
     1921 gataagccac ttattcctgt tcatcatatt gcaggacata tatatgctaa tcacatagaa
     1981 gagccattaa cattcccgct aattgcactt attgtttcag gtggacatac tgaattagtt
     2041 tatatgaagg atcatttatc atttgaagtc attggtgaaa cacgagatga cgcagtaggt
     2101 gaggcttatg ataaagtggc acgaacaatt ggtttaaatt atccaggtgg tccacaagtt
     2161 gatcggttgg ctgctgaagg tgaagatact tattcattcc ctcgtgtttg gttggataaa
     2221 gatagttatg attttagttt tagtgggttg aaaagtgccg taatcaatca acttcacaat
     2281 caacgacaaa aaaatattcc aatcattgaa gctaacgtag caacgagctt tcaaaacagt
     2341 gttgtagagg tgctcacgtt taaagctatt caagcttgta aagaatatgg tgttcagcga
     2401 ttaattgttg ctggtggcgt ggcgagtaat aaaggattac gtcaatcttt agcggatcaa
     2461 tgcaaagtca atgacattca attaactatc ccaagtccta aattatgcac agataatgct
     2521 gcaatgatag gcgttgccgg ccactatttg tatcagcaag gtcgatttgc tgatttagca
     2581 ttaaatgggc acagcaatat agatttagaa gagtattctg cagaataaaa taaacgtcag
     2641 ttattaacat gattactatg aacattattg aaactgttca tagtcttctg tcatgtgata
     2701 gctgacgttt ttatgtttaa agttaaatgc taaattttcg ggacattatc ttttgctcgt
     2761 tcatatattt ttgctggaaa tgaagtgatt tttaataatt cgatggcatt acgtgtattt
     2821 gctttgccag gcttaatttt gtaatcaaaa tgtatgttat tattttctat tacctcattg
     2881 aaatggtaat tttcataacg ttgttttaag agttcagcta actcaatatc atgtgttgct
     2941 gcaataactc taaagttaga tttttcatgt aaaaatgata gtactgattc tgaagcggca
     3001 attcgttcag ttgtgttggt acctttaaaa atttcatcta taaagcagta tattttttga
     3061 ttatctggaa tttcaacaat acgcttaata gattttaatt cagccataaa ataactatcg
     3121 cctgataaca catcatcggc atttgccatt gacgtgaaca caattccagg ttgatataca
     3181 aatttgcttg cggtaactgt ttgtatagcc gatgcaagaa taatgtttat tgctatggat
     3241 ttcataaatg ttgatttacc cgaagcatta gacccagtta acagtatatt ttgtgatagt
     3301 gagaaatcat tggctactgc atctgctatg agtggatgtg ttaactcaga gaatactatg
     3361 ccatcatttg aatcatcaat ttgtggttca gtataacatt ctagcgttct acgatacatt
     3421 gctaatgcat aatgattatc taattcagct atgtaatcga aacaattttt aagctcattc
     3481 atatgtgttg tatagctttt ttgaattgaa tgaaataaaa cataatcaag catgaaaata
     3541 agtttaacta gtttaattac catagctccg atatcttcat cattaacttc agctaaaaca
     3601 ctcgttaaat gacgtgctgt tctaaattgc ttaaaattga cttcaggttg tggagcatgt
     3661 ttaatttttg agatagtata gccttgtttt aaaacattgg atgcataaaa gatagatttt
     3721 aagtcatcct catatgtacg ctttaaagtt gctgataaaa tgatattaaa taggcaactc
     3781 ataagaaata ataaaatacc aacttgtgaa ttaataaata taactgcaaa tgagataact
     3841 ggtaaaaacg ggcatagaaa caatatattt tgacgtttta ccggtgtgat ttggtctgga
     3901 aatgttggat aaacagtttt accaatcaaa gctaaatgat atgttacgtt ttttctaaat
     3961 tcagcattat cattaaataa actaagtaat gatttgttcg taattgaaag cattccacgt
     4021 aaacaggcat atagcttcat ttcaccaata gctgttaaat tataattcat cttatgaaag
     4081 attgcattca tatttaagtc ggaccaagtt ttatcatcaa ctaatgaatg tgattcaaat
     4141 ttttccttgt agagcttata ctcatagtcg aaatgtgagt tcggtctgat aaaagcttct
     4201 aatggagaaa tcgtttgcca taatgtttcc atagatttta atagacgttg acgactcatg
     4261 tatcgtccaa caattcctat tacagatgta agtagaataa ttgctataac aaatattaaa
     4321 aatgtttgat ttgtgctcat aagt
//
LOCUS       DALAAR010000136         4220 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000136 DALAAR010000000
VERSION     DALAAR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.141"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            85..1839
                     /gene="eap"
                     /locus_tag="O2458_002677"
                     /gene_synonym="map"
     CDS             85..1839
                     /gene="eap"
                     /locus_tag="O2458_002677"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCZ3888511.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKNK"
     gene            1826..2140
                     /locus_tag="O2458_002678"
                     /pseudo
     CDS             1826..2140
                     /locus_tag="O2458_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(2464..3750)
                     /locus_tag="O2458_002679"
     CDS             complement(2464..3750)
                     /locus_tag="O2458_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ3888512.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            3950..4048
                     /locus_tag="O2458_002680"
     CDS             3950..4048
                     /locus_tag="O2458_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888513.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT         1608 a          575 c          679 g         1358 t
ORIGIN      
        1 atattcagtt aattcaaaaa atagagaaag tctggctata attaagttgc aaatacgaat
       61 tacacaaaaa aggagagata atttatgaaa tttaagtcat tgattacaac aactttagca
      121 ttaggtgttt tagcatcaac aggtgcaaac tttaataata atgaagcgtc tgccgcagct
      181 aagccattag ataaatcatc aagttcgtta caccatggat attctaaagt ccatgttcca
      241 tatgcaatca ctgtgaacgg tacaagccaa aatattttat caagcttaac atttaataag
      301 aatcaaaata ttagttataa agatttagag gatagagtta aatcagtttt aaaatcagac
      361 agaggtatta gtgatataga tttaagacta tcgaagcaag cgaaatatac tgtttacttt
      421 aaaaatggaa caaagaaagt tatcgatttg aaagcaggta tttacacagc tgatttaatt
      481 aatacaagtg aaattaaagc tattaatatt aacgtagata ctaaaaagca agttgaagat
      541 aaaaagaaag ataaagcaaa ttaccaagtt ccatacacaa tcactgtgaa cggtacaagc
      601 caaaatattt tatcaaactt aacatttaat aagaatcaaa atattagtta caaagattta
      661 gaggataaag ttaaatcagt tttagaatca aatagaggta ttaccgatgt tgatttaaga
      721 ttatcgaagc aagcgaaata tacagttaat tttaaaaatg gaacgaagaa agttatcgat
      781 ttgaaatcag gtatttacac agcgaattta ataaattcaa gtgatattaa aagtatcaat
      841 attaacgtag atacaaaaaa acatatcgaa aataaagcta aaagaaacta tcaagttcca
      901 tattcaatta atttaaatgg tacatctaca aacattttat cgaatctttc attttcaaat
      961 aaaccttgga caaattacaa aaatttaact agtcaaataa aatcagtact gaagcatgat
     1021 agaggtatta gtgaacaaga tttaaaatat gctaagaaag cttattatac tgtttatttt
     1081 aaaaatggtg gtaaaagaat cttacagttg aattcaaaaa attacacagc aaacttagtt
     1141 catgcgaaag atgttaagag aattgaaatt actgttaaaa caggaactaa agcgaaagca
     1201 gacagatatg taccatacac aattgcagta aatggcacat caacaccaat tttatcagat
     1261 ttaaaattta caggtgaccc acgtgtaggc tacaaagata tctctaaaaa agttaaatca
     1321 gtattgaagc atgatagagg tatcggggaa cgtgaattaa aatatgcaaa aaaagcaact
     1381 tacacagtac attttaaaaa tggaacgaaa aaagtgatta acataaattc aaatattagc
     1441 caactgaatc tgctttatgt tcaagatatt aaaaagatag atattgatgt taaaacagga
     1501 actaaagcga aagcggatag ctatgtacca tatacaattg cagtaaatgg cacatcaaca
     1561 ccaattttat caaaacttaa aatttcgaat aaacaattaa ttagttacaa atatttaaat
     1621 gacaaagtga aatctgtatt aaaaagtgaa agaggcatca gtgatcttga cttaaaattt
     1681 gcgaaacaag caaaatatac agtatatttc aaaaatggaa agaaacaagt agtgaattta
     1741 aaatcagaca tctttacacc taatttattt agtgccaaag atattaaaaa gattgatatt
     1801 gatgtaaaac aatacactaa atcaaaaaaa aataaataaa tctaataatg tgaaattccc
     1861 agtaacaata aataaatttg aaaacatagt ttcaaatgaa tttgtgttct ataatgcaag
     1921 caaaattaca attaatgatt taagtataaa acttaaatca gcaatggcaa atgatcaagg
     1981 gataactaaa catgacatag gacttgctga acgcgcagtg tataaagtgt attttaaaaa
     2041 tggttcgtca aaatatgtag acttaaaaac tgagtataaa gatgaaagag tatttaaagc
     2101 aactgacatt aaaaaggtag atattgaact taaattctaa tctttaattt tataataagg
     2161 catctcacaa tcgtggggtg ccttttacat ttgtagagat gtgatgcttg aatgtttgaa
     2221 gtgatttgcc aaaagttata taaattggtt ctataataaa tcggactgat agaaaaagtt
     2281 ttttactttt catctgtccg attttttgat tttgttagga gagatgtata aaactaatta
     2341 ggtatagtgt ttgacaagtt gctgaataaa atatatctta tgatactttg aaagaaggaa
     2401 taattttaaa aataaaaaac caataatccg agtcatactc atcagattat tggttgaaat
     2461 taattatctt aagtcatcaa tcgctttagc aattgaatca aatacatgtg ggatatcttc
     2521 tttttctaca cagctaaacg caatacgaat atctgttgca ttaagtgcaa taacaccaat
     2581 tgaatatttt tcaattaaat gtttacgaag ttcttcaggg tcaacatcat gaacttttat
     2641 tgccataaag tatccagagt taaagtcata ggcttgccaa tgtgaatgat attgatcagc
     2701 atagacaact tccttagtga cttcatagcg ttcttttaat gtttgaatat tttgttcgat
     2761 ttctttatcg aattgtttat tattttttaa tacatgctta acagcgcttt gtgttggaag
     2821 aggtccacta gaaatgttac ttcgtataag accttttact ttggcttcta atacttcttt
     2881 agttgtttga tcagacgttc caaatgtcat aaacccaaca cggaatcccc atgcgaaaaa
     2941 ttcttttgtt gcaccatcta aacgaacagg taatattgca tttgaattta aattagataa
     3001 tgcagtaaat aatgattgag tatacacatc ttcatagaat aaaccatagt atgcgtcatc
     3061 cacaacagct ataacttttg tacctttatc agctaatgct ttaattgcat cgacgatggt
     3121 agtcacttct ttatgcgtag gtgtgtaacc tgtcggatta ttaggataat ttaaaatcat
     3181 aatgacttta tctttattgt atgattgtaa agcttctaca agtgaatcag tagtataatg
     3241 cccgtcttta tcaaagatag gatatgtttg aaggtttgca ccatttctcg tattgaaaac
     3301 aagtttgtaa ttaccccaat tatgctctgg taacaagata gtgtcacctt gatttacaaa
     3361 taaatcgcca actaaagata aaccatgtgt taatgcattc gtaacaattg gtagtgacat
     3421 gttgtcgatt gatagctctg gattgtcacg caacattttt tgttgccata aatcacgtaa
     3481 ttcttcgatg ccttgtggtg gcgcataagg gaatatttca tctggagtta aatcattaaa
     3541 cattgcatct aaagatgatg caaacatttt tccgtctttg tttgtcgcca tacctatagt
     3601 tgcattatat gttgtactct ttgcttcagc agattgagat aaaatgcctt ttggataaaa
     3661 catattttga cctaagtcag ataacattgt aaaggcagtt gcatttgact gttgaagttg
     3721 ttcatttaag ctttgggcta aaggattcat tattgcttaa cctctcttta aaaaaattgt
     3781 atgcctatat attatcttca attataccaa agataaagtg ttgttggtga attaacgaat
     3841 aatatgtaaa atctgctgcc aaaatacgaa catatgttct gtttttatgt tagaataggt
     3901 gtaggtcatc cttcagtatg atactttagt cgaacggagg tgtagtgtaa tgatgtggtg
     3961 gcaagaaggg atgatgacac ttattacagg tggcttacta attatttttc gactttggtt
     4021 agaactgaaa tggaaaaata aaaaatgatc ctacacacat aaataacccc aagtctgctg
     4081 gtaccagact tggggttgtg tagtataatt ctgtggatac acttaaataa tatcattgaa
     4141 cacaatatat cgcaatcaat aatcattttg attgaatggt tttgttgcgc tattttagtt
     4201 gaaagtgatt agtaccttga
//
LOCUS       DALAAR010000137         4196 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000137 DALAAR010000000
VERSION     DALAAR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.142"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            763..2826
                     /locus_tag="O2458_002681"
     CDS             763..2826
                     /locus_tag="O2458_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCZ3888514.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2844..3389
                     /locus_tag="O2458_002682"
     CDS             2844..3389
                     /locus_tag="O2458_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCZ3888515.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3817..3951)
                     /locus_tag="O2458_002683"
     CDS             complement(3817..3951)
                     /locus_tag="O2458_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888516.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            3915..4007
                     /locus_tag="O2458_002684"
     CDS             3915..4007
                     /locus_tag="O2458_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888517.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         1560 a          547 c          740 g         1349 t
ORIGIN      
        1 attgcctcgt ggttctgagc ttgaacttat ctctaaatca ttttgagcta cttatctatc
       61 aattcatata ttctataaca atatttgtga catcacgtgc tatttcatga agtgatttta
      121 cgatatcacc ttctttagaa aaaatatatt tcagtaggca ccgacgtata cagaatcatt
      181 tgagtattaa aaataaaaac tagaaaaaga aaccgcatca ttaactgata cgcagaatca
      241 tattataaat aaaactaaaa atgaggttgt atataactca ctctgaaatt gattgaatat
      301 atagtatctt taataaaatg cagctattgt ggcgtagaat ttgagaatca aaaaatgatt
      361 aatatagttt gaagagactg agcataaata ctagaaaaat ggccagtaaa tgagtttact
      421 ataaactcat ttactggctt ctctaataat tatcaagaca atttgcgttt ctaggcatac
      481 tttgaaatgc gctattttct ttaagaatat taatatatga cttgtgttgg taatgataac
      541 cttggttaga gtgtattgtc atgtgatagt taagattaga tcttttaatt aaaacattgt
      601 tttaaatatc gatgacaagg tctaatgtag gacgtgtaga tataaggttc ggaattatat
      661 aaatccataa aaggagataa atcgaaaacc tttataccct gggtgggtat atgttatagt
      721 ataagtagct ttactataac attttcatta ggaggggtta atttgaataa taatggtgaa
      781 gagcataatc atcaaaatca catgaatcat tccaatcaaa tgcatcatga taaccatgcc
      841 tcacatgatc atcatagtgg ccatgcacat catcatggaa attttaaagt taagtttttt
      901 gtttcattaa tttttgcaat acctatcatt cttttatcgc cactgatggg tgttaactta
      961 ccttttcaat tcacatttcc aggttctgaa tgggtagtgt taatattaag tacaatttta
     1021 ttcttttatg gtggtaaacc gttcttgtct ggtggtaaag atgaaattgc tacaaaaaaa
     1081 ccaggcatga tgaccttagt tgccctaggt atttcagtag cttatattta tagcttgtat
     1141 gctttttata tgaataactt tagtagtgca actggtcata caatggactt tttttgggaa
     1201 ttagcaacct taattttaat tatgctatta ggacattgga tagaaatgaa tgctgtcgga
     1261 aatgctggag atgctttaaa gaaaatggca gaactgttac ctaatagtgc tattaaagtt
     1321 atggataatg gccaacgcga agaagttaaa atatcagaca tcatgactga tgatatcgtc
     1381 gaagtaaaag ccggagaaag cattccaaca gatggtatta tcgttcaagg acaaacatct
     1441 atagatgaat ccctagtcac tggagaatct aaaaaagtac aaaaaaatca aaatgacaac
     1501 gtcatcgggg gttctattaa tgggtctgga acaatacaag tcaaggttac agctgtggga
     1561 gaagatggat atctttctca agttatggga cttgttaatc aagcacaaaa tgataaatct
     1621 agtgctgaat tgttatctga taaagtagcg ggttatttat tctactttgc tgtaagtgtt
     1681 ggcgtgattt cttttattgt ctggatgctc attcaaaatg atgttgattt tgcattagaa
     1741 cgtcttgtaa ctgtgttagt cattgcttgt ccacatgctt taggcttggc aataccttta
     1801 gtcactgcac gttctacttc aattggtgca cataatggtt taattattaa aaatagagag
     1861 tctgtagaaa tagctcaaca tatcgattat gtaatgatgg acaaaactgg tactttaact
     1921 gagggtaact tttctgtgaa tcattatgag agctttaaaa atgatttgag taatgataca
     1981 atattaagcc ttttcgcctc attagaaagt caatctaatc acccattagc tataagtatt
     2041 gttgattttg cgaaaagtaa aaatgtttca tttactaacc cacaagacgt taataatatt
     2101 cctggtgtcg gattagaagg tctaattgat aataaaacat ataaaataac aaatgtctct
     2161 tatcttgata aacataaact taattatgac gatgacttgt ttactaaatt agctcaacaa
     2221 ggtaattcaa tcagttattt aattgaggat caacaagtca ttggcatgat tgctcaagga
     2281 gatcaaatta aagaaagctc aaaacaaatg gtagctgatt tactatcaag aaatattaca
     2341 ccagtcatgc ttacaggtga caataatgaa gtggcacacg ctgtcgcaaa agaattaggt
     2401 attagtgatg tccacgcaca actcatgcca gaagataagg aaagcattat aaaagattat
     2461 caaagtgacg gtaataaagt catgatggtc ggagacggta tcaacgatgc gccgagtctt
     2521 ataagagcgg atattggtat agcaattggt gcaggtacag atgttgcagt ggattcaggt
     2581 gatatcatac ttgttaaaag taatccatca gatatcattc atttcttgac cctttcaaat
     2641 aatactatga gaaaaatggt gcaaaactta tggtggggtg caggttataa tattgttgct
     2701 gtacctttag cagctggtat tttagcattt attggcttga ttttatcacc tgcaataggt
     2761 gctattttaa tgtctttaag tacaattatc gttgcaatta atgcctttac attaaaatta
     2821 aaataaaaga taggagtttt attatgatta aaaaattatt ttttatgata ttaggatcat
     2881 tactaatatt atcagcttgc tccaataatg atgaaaaaga taaagacact aatgaccaaa
     2941 aaagtgagag ccatatgaag cataatgatg aaagtaaagt tccagaagat atgaaatcga
     3001 ctaatgaggg tgaatttaaa gtgggagata aagtaacgat tacagcaggg catatgccag
     3061 gtatgaaagg tgcagaagct actgtaaaag gtgcgtataa aacatatgcc tatgttgtaa
     3121 gttataaacc cacaaatgga aatgaaaaag taaacaatca taaatgggtc gtaaacgaag
     3181 agattaaaga tgcacctaaa gatggattta gtaagggcga tactgttaaa ttagaagcaa
     3241 gtcatatgtc tggtatgaaa ggtgctacag ccaatataga taacgtgaaa aagactactg
     3301 tttacgtagt tgattacaaa tccaaagata atggtaaaat cattaaaaat cataaatgga
     3361 tgacaggaaa tgagctgaaa gcacgataaa aatctagttc taaattgaga aataaataga
     3421 tataaaaata tcctccttaa tcaataattt aaataactta ttattgttaa ggaggatatt
     3481 ttttagtgtg taaattaaaa agaattttag aagaataaca tttatcaaaa aactgttcat
     3541 taccttatta attgaaatta tataattaaa aaccgcatca ttaaccgata cgcagaggcg
     3601 tatcataagt aaaactaaaa aattctgtat gaggagataa taatttggag ggtgttaaat
     3661 ggtggacatt aaatccacgt tcattcaata tataagatat atcacgataa ttgcgcatat
     3721 aacttaagta gtagctaaca gttgaaatta ggccctatca aattggttta tatctaaaat
     3781 gattaatata gaatgcttct ttttgtcctt attaaattat aaaagtaact ttgcaataga
     3841 aacagttatt tcataatcaa cagtcattga cgtagctaag taatgataaa taatcataaa
     3901 taaaattaca gatattgaca aaaaatagta aatataccaa tgaagtttca aaagaacaat
     3961 tccaagaaat tgagaatgta aataataagg tcaaagaatt ttattaagat ttgaaagagt
     4021 atcaatcaag aaagatgtag ttttttaata aactatttgg aaaataatta tcataattta
     4081 aaaactgaca atttgcgaga ctcataaaat gtaataatgg aaatagatgt aaaatataat
     4141 taaggggtgt aatatgaaga ttaatattta taaatctatt tataattttc aggaaa
//
LOCUS       DALAAR010000138         4120 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000138 DALAAR010000000
VERSION     DALAAR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.143"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(488..1765)
                     /locus_tag="O2458_002685"
     CDS             complement(488..1765)
                     /locus_tag="O2458_002685"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCZ3888518.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            2019..3185
                     /locus_tag="O2458_002686"
     CDS             2019..3185
                     /locus_tag="O2458_002686"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCZ3888519.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(3355..3876)
                     /locus_tag="O2458_002687"
     CDS             complement(3355..3876)
                     /locus_tag="O2458_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCZ3888520.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
BASE COUNT         1314 a          801 c          655 g         1350 t
ORIGIN      
        1 tattcttaag tatcttatta ttacttcttt ttaacgctag tggaataatt aataataatt
       61 gtatttaaat gcaatgctgt tgttctttca tacttacaag caagtcataa gaaatgagaa
      121 attaaattct tatatgatga ataaatgatg actgcagaag aaattgctaa aaaaggagtg
      181 gagtgaggca ttctactgtt catattgtta acaaatatca aaatgatagg aacttgaaga
      241 atctaagact gttaaaatcg agacaataag aaactcgttc caaatttgaa tcctataatg
      301 aattcgtctc atgaagaagt tatttacttt tcattagact gatttactaa tcaaaaaact
      361 tctatcacga acgcttcaat ttcctgtatg cgtgtgatag aagtttttat ttttgttcat
      421 attaatttat ctaagcgcta cgatggactg acttaaactt ttctttaaaa tcgctattcg
      481 ccatttacta ttgttgtcta atttcttgta aaatatgttc cgctgcttgt gtatttgcac
      541 ggggttcttt tttcaaagct tcagctactt tagcaatttc atcacctttt gcccctacaa
      601 cgatagctaa tgatttatat tgtaaactca tatgaccttg ttgaatacct tctgacacaa
      661 gcgcgcgaca tgctgcaaag ttttgcgcta aaccaacggc agcaactaca tgacctaatt
      721 cttgtgctga ctctacattt agtagctcta atgaagcttt agcaattggt aatacttttg
      781 taccaccgcc aacgattgcc aatgtcatag gcacttcaat tgtaccaatc aatcgttgac
      841 gatcttgatc gtaacgccat gtagcaatac cacgatactg tccgtcacga ctcgcgtatg
      901 catgcgcact tgcttctgca ccacgcgtat catttcctgt tgctaaaaca acagcatgta
      961 tgccattcat aacaccttta ttatgtgttg ctgcacgatg aatatctact tgggccaata
     1021 cagaagcacg ttccattcgt ttggcaacct cttctccagt tctctcgccc cttgctaaat
     1081 ctttaacatc aatttcgcct tgaactttaa caacggacgc tgttgcatga ttggataaaa
     1141 tactcattaa aatgtcgctt tgcggaaatt cattttttaa aaatgcagtt atggcctcta
     1201 aaatcgtatt aagcatatta gcgcccatag catctttcgt atcaacaaat acttttaaag
     1261 atagtaactg ttgctcagga aatgtatcaa tcgctatacg ttggtaacca ccaccacgcg
     1321 ctttaataga aggatatgcc tcatccgcaa ttttatgaat ttgcttttct aaagctttaa
     1381 tgtctgctga taatttttca gtatcgtcaa cgccatcaaa gacgatttga cctatcataa
     1441 tacgttcaga agataccgtt ttaaatccgc cagtctgatt cactagcttt gcaccataac
     1501 tagctgcagc gacaactgaa ggctcttcca ccatcatagg tacaacatat gccttatcgt
     1561 ccacaatgat attcggtaat aatccaacgg gtaatgcacc ttgcgcgatg acattttcaa
     1621 ttaaactatt ggctacttct tcatcgatta atggatgatt cagtaaaatg tcgaattgtt
     1681 cttctgataa ccattgctta tcaaccaatt gttgtaactt ttctttacga gataaatgtc
     1741 gaaaattctt atctaaattt tgcatggacg tactcctttt acttcacata atttttaaca
     1801 tttcaatcac tactattttt accacaaaat aacgtcattc gtcttaaaat tcaattgaat
     1861 aattgtcgtt ttgactttaa aataaaacaa ggtaaattaa aacgcttaca agaaacgaca
     1921 aatcattttt aaatttagta tatttctttg tataaaatta gcatattctg atatgataca
     1981 agtgttgctt ttataaattt gaaaggatgt aaaaccttat gacaataggt atcgataaaa
     2041 taaactttta cgttccaaag tactatgtag acatggctaa attagcagaa gcacgccaag
     2101 tagacccaaa caaattttta attggaattg gtcaaactga aatggctgtt agtcctgtaa
     2161 accaagacat cgtttcaatg ggcgctaacg ctgctaagga cattataaca gacgaagaca
     2221 aaaagaaaat tggtatggta attgtggcaa ctgaatcagc agttgatgct gctaaagcag
     2281 ccgctgttca aattcacaac ttattaggta ttcaaccttt tgcacgctgc tttgaaatga
     2341 aagaagcttg ttatgctgca acaccagcaa ttcaattagc taaagattat ttagcaacta
     2401 gaccgaatga aaaagtatta gttattgcta cagatacagc acgttatgga ttgaattcag
     2461 gcggcgagcc aacacaaggt gctggcgcag ttgcgatggt tattgcacat aatccaagca
     2521 ttttggcatt aaatgaagat gctgttgctt acactgaaga cgtttatgat ttctggcgtc
     2581 caactggaca taaatatcca ttagttgatg gtgcattatc taaagatgct tatatccgct
     2641 cattccaaca aagctggaat gaatacgcaa aacgtcaagg taagtcgcta gctgacttcg
     2701 catctctatg cttccatgtt ccatttacaa aaatgggtaa aaaggcatta gagtcaatca
     2761 ttgataacgc tgatgaaaca actcaagagc gtttacgttc aggatatgaa gatgctgtag
     2821 attataaccg ttatgtcggt aatatttata ctggatcatt atatttaagc ctaatatcat
     2881 tacttgaaaa tcgagattta caagctggtg aaacaatcgg tttattcagt tatggctcag
     2941 gttcagttgg tgaattttat agtgcgacat tagttgaagg ctacaaagat catttagatc
     3001 aagctgcaca taaagcatta ttaaataacc gtactgaagt atctgttgat gcatatgaaa
     3061 cattcttcaa acgttttgat gacgttgaat ttgacgaaga acaagatgct gttcatgaag
     3121 atcgtcatat tttctactta tcaaatattg aaaataacgt tcgtgaatat cacagaccag
     3181 agtagtcggt gtatttaaaa cacatataat aaaacctaaa agcagcagta agaccacttc
     3241 taattgaaat cgtcttactg ctgttctcta tttataacac ttcgtattga atgaattcat
     3301 tatgcctatt tgacacatta ttgaagtttt cctaatgcct ggatccttta tacgttacgg
     3361 cttcgtgcta tgttttggta cataaagctt tgacatatcg atattctcca actctaacag
     3421 cttaatttta ttattaatcg ttccaccgaa ccctgttaag ctacccgttt taccgacaac
     3481 acgatgacat ggcacgataa tagataatgg attacttccg actgcacctc caaccgcttg
     3541 ggctgacatt tttggcttgt taagcagctt gcctactttt ttggcaatag caccatacgt
     3601 tgttagagtc ccataaggaa cctgtcttaa ttcattccaa acacactgtt gaaaatgact
     3661 acctgttggc tttaaaggta ttgtgatttc aggattgtca cctttaaaat acgcgtctaa
     3721 ccactgtgtc gcctctctaa atatcgctaa agacgtattt tcttccctag taccatcacc
     3781 ttgttgattt tcaaacaaaa cagcggtcag acttacccca tcactcaaaa gttccaatcg
     3841 tcctacaggc gaatcatagt aactcttata ctccataaaa atcccccctt tttctaatca
     3901 tacgccttaa ttgttaaatt agaaaatatt catgttcaca ttttttaaaa ataaataagt
     3961 cgtcaattac gtatataaac acgtaatacc agctatcact ttgctgcaat atacagttac
     4021 atatcttact acacgtgcta acctcttact ttgtaaacca aatcttaaat taaaatattg
     4081 aaaatgcaat gaatccttaa tattttatta aacctataat
//
LOCUS       DALAAR010000139         3920 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000139 DALAAR010000000
VERSION     DALAAR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.144"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            55..1056
                     /gene="argF"
                     /locus_tag="O2458_002688"
     CDS             55..1056
                     /gene="argF"
                     /locus_tag="O2458_002688"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ3888521.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1079..2011
                     /gene="arcC"
                     /locus_tag="O2458_002689"
     CDS             1079..2011
                     /gene="arcC"
                     /locus_tag="O2458_002689"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ3888522.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2183..3739
                     /locus_tag="O2458_002690"
     CDS             2183..3739
                     /locus_tag="O2458_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCZ3888523.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
BASE COUNT         1338 a          553 c          773 g         1256 t
ORIGIN      
        1 attttgtatg cgcttacaat ttaggtgtaa ctaaaataaa aggagttgtt attaatgaaa
       61 aatttacgaa acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc
      121 ttattaacac tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg
      181 ttaaaaaata aaaatattgc actgttattt gaaaaagatt ctacaagaac gcgatgtgca
      241 tttgaagttg cagcgcatga tcaaggtgca aatgtaactt atttaggccc aactggatca
      301 caaatgggta aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat
      361 ggcattgaat accgtggttt ttcacaaaga acagtagaaa ctttagctga gtattcaggc
      421 gtaccagtgt ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc
      481 ttaacagcaa aagaagtctt aaaaaaagat tatgcagata ttaactttac atatgttgga
      541 gatggtcgta ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac
      601 ttccatttag tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa
      661 aatattgccg ctgaaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta
      721 aaaggttcgg atgtaattta cactgatgtt tgggtatcaa tgggtgaacc tgatgaagta
      781 tggaaagaac gacttgaatt attgaaacca tatcaagtaa ataaagaaat aatggataaa
      841 actggtaatc caaatgttat ttttgagcat tgcttaccat ctttccataa tgctgatacg
      901 aaaataggtc aacaaatttt tgaaaaatat ggtattcgag aaatggaagt tacagatgaa
      961 gtattcgaaa gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc
     1021 aaagcagtca tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat
     1081 ggcgaaaata gtagtagcat taggtggtaa tgctttagga aaatcacctc aagaacaact
     1141 cgagcttgtt aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat
     1201 tgttattagt catggtaatg gaccacaggt tggaagcatt aatttgggac ttaactatgc
     1261 tgcagaacat aaccaaggtc cggcatttcc atttgctgaa tgtggcgcaa tgagtcaagc
     1321 ttacatcggc tatcaattac aagaaagctt acaaaatgaa ttgcattcta ttggaatgga
     1381 taaacaagtg gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa
     1441 caatccttca aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa
     1501 agaaaaagga tttatatttg ttgaagatgc tggaagagga tatagacgcg ttgttccttc
     1561 accacaaccc atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac
     1621 actcgttatt gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt
     1681 taaaggtatt gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat
     1741 tcaatgcgat caattgatta ttttaacagc aattgattat gtatatatta attttaacac
     1801 tgaaaaccaa cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga
     1861 aaatcaattt gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga
     1921 aaacaatcca aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga
     1981 gggtaaagta ggtactgtga ttaaaaagta attgaattga aacgcttttc aattactata
     2041 tgtcaaatgc atgattttta ttatttatgt gcaccccctg aaaataatgc ctctattttg
     2101 atgcggggtg cactttctta atttatattt ataaaatctt taaggtagaa atgctaggtt
     2161 aaatgtcgaa ggagatgaaa ccgtggaaaa tacaattaat gaaagtgaaa agaaaaaacg
     2221 atttaaatta aaaatgccag gtgcatttat gattttattc attttaacgg ttgttgcagt
     2281 tatagcaaca tgggttattc ctgctggtgc atattctaaa ctttcttacg aaccttcatc
     2341 ccaagaacta aagatagtta accctcataa ccaagtgaaa aaggttccgg gtacgcaaca
     2401 ggaactagac aaaatggggg ttaaaattaa gattgaacaa tttaaatcag gtgcaattaa
     2461 taagccggta tcaattccga atacttatga aagattaaag caacatccag ctggaccaga
     2521 acaaataaca agtagcatgg ttgaaggtac gatagaagcg gtcgatatca tggtattcat
     2581 tcttgtacta gggggactta ttggcgtagt tcaagccagt ggttcttttg aatcgggatt
     2641 gttagcttta acgaagaaaa caaaagggca tgaatttatg ctaattgtgt ttgtatcaat
     2701 actaatgatt atcggcggga cattatgtgg tattgaagaa gaagctgtag cattttatcc
     2761 gattttagtc cctatattta tagcgttagg atacgattct atcgtttcag ttggtgccat
     2821 attccttgcc agctctgtcg gtagtacatt ttcaactatt aacccgttct cggttgtaat
     2881 tgcctctaat gccgctggta caacttttac ggatggcttg tattggagaa taggtgcttg
     2941 tattgtcggt gcgatttttg ttattagtta tttatattgg tactgtaaaa aaattaaaaa
     3001 cgatcctaaa gcgtcatatt cttatgaaga caaagatgct tttgaacagc aatggtctgt
     3061 attaaaagat gacgatagtg cccattttac tttgcgtaag aagataatcc ttacattatt
     3121 tgtactacca tttccaatta tggtatgggg agttatgacg caaggttggt ggttcccagt
     3181 tatggcttca gcatttttaa tatttacaat tataataatg tttattgctg ggacaggtaa
     3241 atctggattg ggagaaaaag gaactgtaga tgcatttgtc aatggtgcat caagtttagt
     3301 aggtgtatct ttaattattg gtttagctcg aggtattaat ttagtgttga atgaaggtat
     3361 gatttcagat acaatcttac acttttcatc atctttagtt caacatatga gtggaccatt
     3421 atttatcatc gtattactat ttattttctt ctgtttaggt tttatcgtgc catcatcttc
     3481 tggcttagcg gtattatcaa tgcctatctt tgcaccacta gctgatacag taggtatacc
     3541 aagattcgtc atcgttacga catatcaatt tggtcaatat gcgatgttat tcttagcgcc
     3601 gactggactt gttatggcca cactacaaat gttaaacatg cgatattcac attggttccg
     3661 atttgtatgg ccggtagttg cttttgtatt gattttcggt ggcggagtac taattacgca
     3721 agtactaatt tattcataat ttgaaatgct atattataaa aaatactaat ggggttttat
     3781 gcatctcgta ggtttgtaga aatactaaac taagcgaggt gcattattat ttttgattaa
     3841 gaaaataatg acggtaatga taacactagt aagtaattga tacaatgctc tatttaataa
     3901 tgatattttt aaaatttgtt
//
LOCUS       DALAAR010000140         3814 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000140 DALAAR010000000
VERSION     DALAAR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.145"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            53..577
                     /locus_tag="O2458_002691"
     CDS             53..577
                     /locus_tag="O2458_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888524.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            637..1194
                     /locus_tag="O2458_002692"
     CDS             637..1194
                     /locus_tag="O2458_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCZ3888525.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            1191..2033
                     /locus_tag="O2458_002693"
     CDS             1191..2033
                     /locus_tag="O2458_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCZ3888526.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2090..3130)
                     /locus_tag="O2458_002694"
     CDS             complement(2090..3130)
                     /locus_tag="O2458_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ3888527.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            3581..3754
                     /locus_tag="O2458_002695"
     CDS             3581..3754
                     /locus_tag="O2458_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888528.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         1335 a          475 c          661 g         1343 t
ORIGIN      
        1 taattgcaaa ttctttgtta atttttgtta aggatgaaac gggaagcacc ttatgctata
       61 tttaaataag tacaaagaaa gggtgacatc agtgcgtatt caaaatcgct gggttgtgtt
      121 tatattattt ttaatctgtt cttttggtgt attaattggt ttatatcaat atcgtcatac
      181 gaaaactgtg gatttgtcta atcttgaaat aaacgatatt aaattaaatg agaagtttga
      241 taaaaaaggc tatgaagtaa ataaaaaaat taagtttgat cgttttaaat tttataacag
      301 taaagctcac cctgatctta ccgttaaagt gagagaaaag gataacatcg ttaaggggat
      361 aatattagta agagatgaaa agatacatac taattttgat gggggaattg gttcgccgat
      421 aaataacgcg attgaaaatc ttggattcgg atataaaaga acaaaagttg gcaatgattt
      481 ctcatcggta aagtatattg atagagataa ccatttaaaa ttaaacttac tttatcaaga
      541 tttagaaatt aaacgtattg aattttttag taaatagctt taggtcttaa agttataaaa
      601 aacgaatgaa taattttatt gggatgagtg acctatatga acaagattac ttatttaaat
      661 gaattagaaa cagcacttga tgcgctacct aggcatttaa gagatcaaaa aatgtatgaa
      721 tatgaacgtt atttttatga gcaagaactt aatggcgttg atgaagggga aattttaaag
      781 aagttaaaag acccacaaga tgttgcagct gaaacaaaag ctagaagtgt cattgattat
      841 gctgaatcta aaccaacatt tgaaaatatt tcaagagctg ttgctgcttc attaagttta
      901 ggcattctat ctatttttgt catccttata ccagtatcta tagttggatt atttgtatta
      961 gcattatttt taatatcact tttgctgctg ttttgtccaa ttattttatt agcatcagca
     1021 atatccagag gaattgtgga ctcaattagt aatgtatttt ttgccatatc atattcagga
     1081 ttaggattag tatttatcat tgtcatattt aagattttag aatacattta tcgtttaatc
     1141 ttaaaatatt tactttggta tattaaaact gtcaaaggaa gcgttagaaa atgaagaaat
     1201 tcttttttat tgggctttta gtgtttgttg tcttttttac agcagcaacc attatttggt
     1261 tcagctatga taaaaacaaa tatggtacta aacaatatga taaaacattc aaagacgatg
     1321 cttttgacaa tgtatctata aatttggata gtacagaact tcgtataaaa cgggggaatc
     1381 aatttagagt taaatatgat ggtgacaatg atatattaat taatatagta gataagacgt
     1441 tgaagattag tgataaaagg tctaagacaa gaggatatgc aattgatatg aatccttttc
     1501 atgagaataa gaaaacgtta acgattgaaa tgcctgataa aatgattaaa cgtttaaatc
     1561 tatcatctgg agcaggaagt gttagaatca gtgatgttga tttagagaac acaagtattc
     1621 aaagcattaa cggtgaagta gttattaaaa actcaaatct tgacgcacta gattcaaaga
     1681 caaataatag ttctacttat attagtaaaa gtaacattaa aaatagcaat attaaagttg
     1741 ttattggtac gctacaaatc gacaagagtc aaattaaaca atccatattt ttaaacgatc
     1801 atggtgacat tgaatttaaa aacatgccat caaaagtaga tgcaaaagct tctactaaac
     1861 aaggagatat tcgttttaag tatgatagta aacctgaaga cactatacta aagctaaatc
     1921 cgggaacggg tgatagcgta gttaaaaata aaacatttac taatggtaaa gttgggaaaa
     1981 gcgacaatgt tttagaattt tatacgattg atggtaatat caaagttgaa taaataaagg
     2041 atgtaagcac cgatattagg aagcataatt tctctaatat cggtgttatt tatttgttgg
     2101 caaaagttaa gtcggtatct atattgccag taaagtgagt gatattaagg tcttgaccat
     2161 ctaaccatga tttgaaatct attatttctg gtggcgcatt ttctcccaat gtaaaatatg
     2221 cagttaatgt ttcaggttga tacattgatg tatggatggt gccagaccag cttttgaata
     2281 gtttactgta aatttcatac tgaggattat tgaataactt aaatgctgta gtcatatcta
     2341 aattatcatt agtttgtgaa atggtacgcg ccagtctttc tttagattct tttgtataat
     2401 tacgattttc atgtgttaat atttcaaaat gatttgtaca tatattatca taacgaacat
     2461 ctattgatct cggtgtcact tcaacaattg catggttcaa tgatttgtcc atcagtatgt
     2521 agctaaatga gcttctgtgt ggtatttctt tcaataattg gattgcttct gttacatttc
     2581 ggcaattttc aagaattaga cgaccaatca tataacatac aaaaccattt gctggtttct
     2641 tccggtgcat aaagttatag cccatagtta atcctgactc attcatacca tccattcttc
     2701 cagttaccct tgatacagga ccaatttgag ctaaaccgct atctgtaggt tgataaagta
     2761 agtagcgacc atcataagtt gcagggtggt aatcataatt tctaaccatg aagtctttgc
     2821 cttgaaagac cgtgcaacca ctttctttta aatcggtaaa acgataatgt ccaaagttta
     2881 aaataatttg gcgtgttggc attttgagta tactttgtag tcccattaat tcttcccata
     2941 tttgaggtgc gtatgtttgg aatatttgat aagtttcatt tacatctata tcgaaacgtg
     3001 ggacacgttt tttccattct ttttctcgat tttttagaag aggtgtttgt tgaagccatt
     3061 taccagtttt aacacctaac tcgaaatgtg aacctctaaa agtcatgata tctgatgtca
     3121 cttgttgcat atcatcggcc cctttctttt tagttgtaat atattgtaaa taaatagtaa
     3181 tcgtatgtat attgaatgtc atgttaaata aagttatatt ttactaaatg aaatataaaa
     3241 ttgtttgagg tgatttctcg gtgtataaga cttatcaatc agttaaaaca tatttttata
     3301 gatggtgggg atattgagtt aaaaacttaa aatcatctta tcataaatat caatcttaag
     3361 ttagcattca cgataatagt cattgttaac attagcatat aaggtcatgt cacgttgaaa
     3421 cagaggttcc tcggcatttt tgaaaataat cattagcttg ctcactatat aatttgatga
     3481 atatatttcg tgaaagtggg tatttattta atgattattc tatatatgat agtataatga
     3541 aatgtagata ggtatttaat ttaacagagg tgaaattgag atgtggaatt ttattaaatg
     3601 tgtgtttaaa ttcgtattta gcttagttgc tattacaaca ttagttgctg gtgttggtgt
     3661 agtagcattt gcttatatct ttaaaaaaga ttttgaagat attgaaagaa aaactaaaga
     3721 aattatttct gatattgaaa gtaaaaataa ctaataacat ttagaggctg ggacataaat
     3781 ccctaaaaaa cagcagtaag ataattttca atta
//
LOCUS       DALAAR010000141         3648 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000141 DALAAR010000000
VERSION     DALAAR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.147"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            235..1470
                     /gene="arcA"
                     /locus_tag="O2458_002696"
     CDS             235..1470
                     /gene="arcA"
                     /locus_tag="O2458_002696"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCZ3888529.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1556..2977
                     /gene="arcD"
                     /locus_tag="O2458_002697"
     CDS             1556..2977
                     /gene="arcD"
                     /locus_tag="O2458_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCZ3888530.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGNISVF"
     gene            3019..>3648
                     /locus_tag="O2458_002698"
     CDS             3019..>3648
                     /locus_tag="O2458_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCZ3888531.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQ
                     YKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPA
                     PYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRASSA
                     KERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIR"
BASE COUNT         1311 a          482 c          648 g         1207 t
ORIGIN      
        1 aatacaaaaa aatgagtaaa aaatcttttt gcttgaataa gataaggttt taacaatgag
       61 atttttcaaa ttattcacta ttatatagta caaaatacat attcatacaa aattttgcat
      121 tgaataaatc tatatatgtg aaaaccctcc ctaaacaatt gtgaattaaa tcacacgatg
      181 ttttttgaaa gcgttttata atgtgaatgt aataaagcaa aggaggaatt tgaaatggta
      241 caaggaccca ttcaagtaaa tagtgaaata ggcaaattga aaactgtgtt gttaaaaaga
      301 ccaggaaaag aattagaaaa tttagtacct gatcatttaa gtggtttatt attcgatgat
      361 attccctact taaaagttgc acaagaagag catgacaaat ttgctcaaac tttgagagat
      421 gaaggaatcg aagtagttta tttagaaaaa cttgcagcag aatctattac tgagccagaa
      481 gtacgcgaga acttcataaa cgacatatta acagaatcta aaaagacaat attaggtcat
      541 gaaactgaaa ttaaagaatt cttttcaaag ttatctgacc aagaacttgt aaataaaatc
      601 atggctggcg tacgtaaaga agaaattcaa cttgaaacaa cccatttagt agaatatatg
      661 gatgatagat atccatttta cttagatcca atgcccaacc tttattttac aagagatccc
      721 caagcttcaa ttggtagagg aatgacaatt aacagaatgt attggagagc acgacgtaga
      781 gaatctattt ttatgacata tatactgaaa catcatccaa gatttaaaga taaagatgta
      841 ccagtatggt tagatcgtaa ctcaccattt aatattgaag gtggagatga attagtatta
      901 tcgaaagatg ttttagctat tggtatatca gaacgtacat cagctcaagc aatagaaaag
      961 ttagcacgta atattttcaa agatgcaaac acaagtttta aaaaaatcgt agctattgaa
     1021 atacctaata cacgtacatt tatgcaccta gatacagtac taactatgat tgactacgat
     1081 aagtttacag tacatgcagc aatatttaaa gaagaaaata atatgaatat atttaccata
     1141 gaacaaaatg atggtaagga cgatataaaa attactcgtt ctagcaagtt acgtgaaaca
     1201 cttgctgaag ttttagaagt agaaaaagtg gactttattc caacaggtaa tggcgacgtt
     1261 attgatggtg cacgtgaaca atggaatgat ggctcaaaca cattatgtat tcgaccaggg
     1321 gttgtggtga catacgatcg caactatgta tcaaaccaac ttttacgcga caaaggaatt
     1381 aaagtgattg aaattactgg tagtgaactt gtacgtggac gcggaggccc aagatgtatg
     1441 agtcagccgt tatttagaga agatatttaa atttgaaagg tggtaaaagt cacaagtaat
     1501 tcattcatta aatcaaataa caagattttt accaccttta ttaggggaga ttaatatgga
     1561 tgaaaataaa ttaggtaaaa cttccttaat tggtttagtc ataggctcta tgataggcgg
     1621 tggtgcattc aatatcatct cagatatggg tggccaagct ggtggacttg caataattat
     1681 cggttggata ataactgcta ttggtatgat ttctcttgct ttcgtatttc aaaatttaac
     1741 aaatgagcga ccagatcttg atggaggaat ttatagttat gctcaaacag ggtttggaga
     1801 ttttattggt ttttcaagtg cttggggata ttggtttgca gcatttctag gtaatgtggc
     1861 ttatgcaacc ctattaatgt cagctgtggg taactttttc cctatattta aaggaggtaa
     1921 cacacttcca agtattatca tagcatcaat tttattatgg ggtgtacatt ttttaatact
     1981 tagaggtgta gaaactgcag cgtttataaa tagtattgtt acagtagcta aattaatacc
     2041 tatatttcta gttattatat gcatgatagt tgtattcaac ttcagtactt ttaaatccgg
     2101 tttttatggt atgactagtg gaagtgttgg cgtttttagt tggggagata caatggcaca
     2161 agtaaaaagt actatgttag taactgtatg ggtattcaca gggattgaag gagccgttgt
     2221 cttttctgga cgtgcaaagt ctaaaaagga tgtaggaact gctaccgtta ttggtttgat
     2281 ttctgtgcta gtcatttatt tcttaatgac tgtactagcc caaggtgtca ttcagcagaa
     2341 ccaaatttca aaacttgcta atccatcaat ggcacaagta ttagaacata ttgtaggtca
     2401 ttggggttca gtgttagtta atataggctt aattatctct gttttaggag cttggttagg
     2461 atggacatta ctagctggtg aattaccatt cattgtagct aaagatggac ttttcccgaa
     2521 atggtttgct aaagaaaata agaataaagc tccggtcaac gctttaatta ttactaatat
     2581 attagttcag ttatttttaa ttagtatgtt gtttacagat agtgcctatc agtttgcgtt
     2641 ttcacttgca tcaagtgcaa tcttaattcc atatacactc agtgcttttt accaggttaa
     2701 atatactatt caaaataaat ctaaagctaa tttaaaacaa tggataatag gaattattgc
     2761 atctatttac acaatttggt tggtttatgc agctggatta gattatttac tattaacgat
     2821 gttgttatat atacctggat tactcgtata cagctacgta caaagggata ataacaaaca
     2881 tttgacaaaa ttggattata cgttattcat attcatcatt gtacttgcaa taataggaat
     2941 agttcgtttg attacaggta atatttctgt attttaagtt atgatttaaa ctaagtaatt
     3001 tttaaggggt ttatagttat gtatgaagaa aatatttata ttaaaaattc agaatatgaa
     3061 tttgataata atcttaaaca attagcatca tacttaaata ttcctgttag tattgttaga
     3121 ccttataaag aggatttaac actttatcaa tataaaaaag gacaagtcat atatcattca
     3181 actgatcaaa taaaatttgt atacttttta gtaaatggtt gtattttaca tgaatcttct
     3241 aatattactg gtgacaatta tttaagatta agtaaagacg aaaatatatt tccaatgaac
     3301 ttcatattta atgaaacccc tgcaccatat gaaatatgta cagctttgac agattgtaaa
     3361 atattaactt taccgaaaga tttacttgag tatttatgta gaaagcataa tgaaatattt
     3421 gaaagtctct tcaagaaact taatgagact attcaatttc aagtagaata tattatggcg
     3481 ttaagagcta gttcagctaa agaaagaatt gaaagaatac tacaaatttt atgcctttca
     3541 attggggatg ataatggaga attctatgaa ttaaaacaaa ttatgactgt tcaattaata
     3601 agtaatttat ctggacttaa cagaaaaact actggtgaaa taatcaga
//
LOCUS       DALAAR010000142         3574 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000142 DALAAR010000000
VERSION     DALAAR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.148"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..297
                     /locus_tag="O2458_002699"
     CDS             <1..297
                     /locus_tag="O2458_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ3888532.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O2458_002700"
                     /pseudo
     CDS             355..732
                     /locus_tag="O2458_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O2458_002701"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O2458_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O2458_002702"
     CDS             1726..2655
                     /locus_tag="O2458_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888533.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O2458_002703"
     CDS             2648..3385
                     /locus_tag="O2458_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888534.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="O2458_002704"
     CDS             3399..>3574
                     /locus_tag="O2458_002704"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCZ3888535.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1308 a          491 c          632 g         1143 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DALAAR010000143         3559 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000143 DALAAR010000000
VERSION     DALAAR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.149"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            132..1043
                     /locus_tag="O2458_002705"
     CDS             132..1043
                     /locus_tag="O2458_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ3888536.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1105..1950
                     /locus_tag="O2458_002706"
     CDS             1105..1950
                     /locus_tag="O2458_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ3888537.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(2322..3545)
                     /locus_tag="O2458_002707"
     CDS             complement(2322..3545)
                     /locus_tag="O2458_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCZ3888538.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT         1321 a          487 c          561 g         1190 t
ORIGIN      
        1 ttaataatta gataatatca taaatgaaaa tagaaatgtt actgatgtgt attactaaca
       61 ggcgttgact aattctttta atattttata attgaaaatg aatatcaatt gaaaaacaaa
      121 aggatgatat tatgaaaaga ttaattggaa ttttattatg taatttattt attttgactg
      181 cgtgttcagc atctgtcgat aaaacaagta actcgacaaa gactacagac tataaaattg
      241 aaaatggtga aacactgaaa gtaccagaga aacctaaaag agttgctgta ttaactggat
      301 tttatgttgg tgattttata aagttaggaa tcaaaccaat tgctgtttca gatataacta
      361 aagattcttc aattttaaaa ccttatttaa aaggggttga ttatattgga gaaaatgatg
      421 ttgaaagagt tgctaaagca aaaccagatt taattgttgt agatgctatg gataaaaata
      481 taaaaaaata ccaaaaaata gcgccaacaa tcccatatac atacaataaa tacaatcata
      541 aagaaatatt aaaagaaata ggcaagttga ctaataatga agataaagct aaaaaatgga
      601 ttgaagagtg ggacgataaa actagaaaag ataaaaaaga aattcaaagt aaaattggtc
      661 aagcaacagc atctgtgttt gaaccagatg aaaagcaaat atacatatat aactctacat
      721 ggggtcgtgg tttggatatt gttcatgatg cattcggtat gccaatgaca aagcaatata
      781 aagataaatt acaagaagat aaaaaaggct atgcttccat ttcaaaagaa aatattagta
      841 aatatgctgg tgattatata tttttaagta aaccttcgta cggaaaattt gattttgaaa
      901 aaacacatac atggcagaat attgaagctg taaaaaaagg acatgtaatt tcatataaag
      961 cagaagatta ttggttcaca gatcctatta cattagaaca tttgagaagt aaattaaaaa
     1021 aagaaatttt aaataaaaaa taatagaaat aagttgtaaa aattttctta tgcattggta
     1081 ctaatgtttt taaggagtga ttaaatgaag caactggttg gaattcccga atcaatgtta
     1141 attcctttga tagctcgagc aaaagagtac gaaaacgaaa aaccaataat aaaagacgca
     1201 ctatcaaaaa aaatatttga tggtttagat gatatgtaca aaaatgttac atgtgatgac
     1261 atgtctcaaa ttggaattag tatacgtact gtgataatag attgtgttac taagaggctt
     1321 atcaaggata ataaagattt aatcgtggtc aatataggtt gtggcttaga tacaaggttt
     1381 caaagattta ataaagaaaa aatatcatgg atagatttag atgtaccaga atcaatagaa
     1441 atacgaaaaa cattttttaa agaaagtaat agttataaga tgatatctaa atctatgcta
     1501 gattacagtt ggattgatga tgtcaaaaat tataaatttt ttaatagtaa gtcaaatata
     1561 ttgtttatta ttgaaggtgt attgatgtat tttgatgaga gtgtaatgac tcaattattg
     1621 gacactatta tcaaaaagat gggagatcat aatttgacat ttgcgattga attttgctca
     1681 aaaacaattg cgaataatac aaagagacat caatcggtat caaagttatc ctcacaacct
     1741 gtttttaaat atggttacaa tgatttaaaa aaattgaatg aaattttacc aaatacaata
     1801 agagtaatac atgaatataa ttattttgac tattataaga atcgatgggg cttatttgga
     1861 tattgtagat ttatacctta tttaaaaaaa aggttaaaca ataaaatagt acttatgaaa
     1921 tataaagttc caaagagaca gagacattaa gttcttagac aatgtaaaaa agctgatttc
     1981 tataaattat ttgatagaaa tcagcttttt tcaaatgtat ttgataattt acagctcgtt
     2041 gagctgctgt tttctaggga tttatgtccc attcctttta cgagaaggtt tttacgagtt
     2101 ttatgtaact attagaagct atctttttat atgaccatta gtcaatatat gtatgacaaa
     2161 tcagcacaat aatttcagtt ttacatcact taatcaatga aaatgtgctt gttgatattt
     2221 gaatatttac attcaaacaa tattcatcgc tatttctatc aacttaaaat tcgcaataaa
     2281 aaactgccag cacgcgaata gggcagctga cagcatcgtt cttatttttc ttttaaatat
     2341 tgaatggaag cctctttaaa aatatcaata tatttcagat acatatcttt ttcaatatat
     2401 tcatcgattt gatgtgccat taatggatta cctggtccaa aaatggctaa atcaacattg
     2461 tccttattat ctcctaagaa actagaggca tctgttgcac ctacaagcgc tgaaacaaat
     2521 atttcgtctt gttctacata actagaagct acatctttaa tcgtagtaat taatttgcta
     2581 tttttatcgc ttgttacagg tcggtgattg cttggaatat cgagtgaaag cttattgcta
     2641 tccacatcat taatgatatt ttggaaaaac gattctataa agtcgttatc atactcagga
     2701 actggtctta cgttaaattc aagtgaagct tcatctggta cagagttaaa ttgtttgccg
     2761 ccatttataa tcgaacatac agctgtaaga ccagatgcat aatttgcatc ctcttcagaa
     2821 atttcttttc caatcaatga tttgaacata ggcgcaacat ctaattcatg tttagtatct
     2881 tgttgtttaa gctctgaata tttttcttta aatagattat aaaattcaag cagtgtatca
     2941 attgcattgt caccaataaa tggaactgag ctatggacag ctttaccagt tgcagttact
     3001 ttacatgaca tagacccctt atgtgcataa taaattccag atccagttgg ttcagcaata
     3061 attaagctat cgacatcgtc taaatagcct ttatcagcta ataatttggc accttcttgt
     3121 tctttctctt cgccagcagt agccagtaat ctaatcgttc catgaggcaa ttcattttgt
     3181 tcttttaatt cgattagaga tacgaccaaa gccattaaac cgcctttcat atctgtagtg
     3241 cctcggccat ataatttgcc atctttttct gtcagttgaa aagggggata tgaccaatta
     3301 tcttgatttc ctgcatcaac aacatccata tgaccactca atgcgagtat aggtgagccg
     3361 ttaccgattt ctgcaacgat attggcgcgg tgttcattaa ctttcaaaat ttcagattta
     3421 atatcgtact tgtcgaataa atctgttaaa taattacaaa cgtctatttc attattattt
     3481 tcagtttgta gttcaacaat atctgctagt aattgaattt tttctttttc actaaaagtt
     3541 gtcattaagc tcacacctt
//
LOCUS       DALAAR010000144         3533 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000144 DALAAR010000000
VERSION     DALAAR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.150"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            166..468
                     /gene="gatC"
                     /locus_tag="O2458_002708"
     CDS             166..468
                     /gene="gatC"
                     /locus_tag="O2458_002708"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCZ3888539.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            470..1927
                     /gene="gatA"
                     /locus_tag="O2458_002709"
     CDS             470..1927
                     /gene="gatA"
                     /locus_tag="O2458_002709"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCZ3888540.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            1940..3367
                     /gene="gatB"
                     /locus_tag="O2458_002710"
     CDS             1940..3367
                     /gene="gatB"
                     /locus_tag="O2458_002710"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCZ3888541.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            3407..>3533
                     /locus_tag="O2458_002711"
     CDS             3407..>3533
                     /locus_tag="O2458_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888542.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALI"
BASE COUNT         1305 a          519 c          664 g         1045 t
ORIGIN      
        1 tctaatatta aaagagtgtt gttttcataa aaaatgattt gataaacgaa tgataatgtt
       61 tatacagttt atatgaaatt aaagtagcac ctcataaata cttagatttt taattggaaa
      121 tttgatacaa tttagtgatg aatgacttaa aggaggcttt tattaatgac aaaagtaaca
      181 cgtgaagaag ttgagcatat cgcgaatctt gcaagacttc aaatttctcc tgaagaaacg
      241 gaagaaatgg ccaacacatt agaaagcatt ttagattttg caaaacaaaa tgatagcgct
      301 gatacagaag gcgttgaacc tacatatcac gttttagatt tacaaaacgt tttacgtgaa
      361 gataaagcaa ttaaaggtat tccacaagaa ttagctttga aaaatgccaa agaaacagaa
      421 gatggacaat ttaaagtgcc tacaatcatg aatgaggagg acgcgtaaga tgagcattcg
      481 ctacgaatcg gttgagaatt tattaacttt aataaaagac aaaaaaatca aaccatctga
      541 tgttgttaaa gatatatatg atgcaattga agagactgat ccaacaatta agtcttttct
      601 agcgctggat aaagaaaatg caatcaaaaa agcgcaagaa ttggatgaat tacaagcaaa
      661 agatcaaatg gatggcaaat tatttggtat tccaatgggt ataaaagata acattattac
      721 aaacggatta gaaacaacat gtgcaagtaa aatgttagaa ggttttgtgc caatttacga
      781 atctactgta atggaaaaac tacataatga aaatgccgtt ttaatcggta aattaaatat
      841 ggatgagttt gcaatgggtg gttcaacaga aacatcttat ttcaaaaaaa cagttaaccc
      901 atttgaccat aaagcagtgc caggtggttc atcaggtgga tctgcagcag cagttgcagc
      961 tggcttagta ccatttagct taggttcaga cacaggtggt tcaattagac aaccggctgc
     1021 atattgtggc gttgtcggta tgaaaccaac atacggtcgt gtatctcgat ttggattagt
     1081 tgcttttgca tcttcattag accaaattgg tccattgact cgaaatgtaa aagataatgc
     1141 aatcgtatta gaagctattt ctggtgcaga tgttaatgac tctacaagtg caccagttga
     1201 tgatgtagac tttacatctg aaattggtaa agatattaaa ggattaaaag ttgcattacc
     1261 taaagaatac ttaggtgaag gtgtagctga tgacgtaaaa gaagcagttc aaaacgctgt
     1321 agaaacttta aaatctttag gtgctgtcgt tgaggaagta tcattgccaa atactaaatt
     1381 tggtattcca tcatattacg tgattgcatc atcagaagct tcgtcaaacc tttctcgttt
     1441 tgacggaatt cgttatggtt atcattctaa agaagctcat tcattagaag aattatataa
     1501 aatgtcaaga tctgaaggtt tcggtaaaga agtaaaacgt cgtattttct taggtacatt
     1561 tgcattaagt tcaggttact atgatgctta ctataaaaaa tctcaaaaag ttagaacatt
     1621 gattaaaaat gactttgata aagtattcga aaattatgat gtagtagttg gtccaacagc
     1681 gcctacaact gcgtttaatt taggtgaaga aattgatgat ccattaacaa tgtatgccaa
     1741 tgatttatta acaacaccag taaacttagc tggattacct ggtatttctg ttccttgtgg
     1801 acaatcaaat ggccgaccaa tcggtttaca gttcattggt aaaccattcg atgaaaaaac
     1861 gttatatcgt gtcgcttatc aatatgaaac acaatacaat ttacatgacg tttatgaaaa
     1921 attataagga gtggaaatca tgcattttga aacagttata ggacttgaag ttcacgtaga
     1981 gttaaaaacg gactcaaaaa tgttttctcc atcaccagcg cattttggag cagaacctaa
     2041 ctcaaataca aatgttatcg acttagcata tccaggtgtc ttaccagttg ttaataagcg
     2101 tgcagtagac tgggcaatgc gtgctgcaat ggcactaaat atggaaatcg caacagaatc
     2161 taagtttgac cgtaagaact atttctatcc agataatcca aaagcatatc aaatttctca
     2221 atttgatcaa ccaattggtg aaaatggata tatcgatatc gaagtcgacg gtgaaacaaa
     2281 acgaatcggt attactcgtc ttcacatgga agaagatgct ggtaagtcaa cacataaagg
     2341 tgagtattca ttagttgact tgaaccgtca aggtacaccg ctaattgaaa tcgtatctga
     2401 accagatatt cgttcaccta aagaagcata tgcatattta gaaaaattgc gttcaattat
     2461 tcaatacact ggtgtatcag acgttaagat ggaagaggga tctttacgtt gtgatgctaa
     2521 catctcttta cgtccatatg gtcaagaaaa atttggtact aaagccgaat tgaaaaactt
     2581 aaactcattt aactatgtac gtaaaggttt agaatatgaa gaaaaacgcc aagaagaaga
     2641 attgttaaat ggtggagaaa tcggacaaga aacacgtcga tttgatgaat ctacaggtaa
     2701 aacaatttta atgcgtgtta aagaaggttc tgatgattac cgttacttcc cagagcctga
     2761 cattgtacct ttatatattg atgatgcttg gaaagagcgt gttcgtcaga caattcctga
     2821 attaccagat gaacgtaaag ctaagtatgt aaatgaatta ggtttacctg catacgatgc
     2881 acacgtatta acattgacta aagaaatgtc agatttcttt gaatcaacaa ttgaacacgg
     2941 tgcagatgtt aaattaacat ctaactggtt aatgggtggc gtaaacgaat atttaaataa
     3001 aaatcaagta gaattattag atactaaatt aacaccagaa aatttagcag gtatgattaa
     3061 acttatcgaa gacggaacaa tgagcagtaa aattgcgaag aaagtcttcc cagagttagc
     3121 agctaaaggt ggtaatgcta aacagattat ggaagataat ggcttagttc aaatttctga
     3181 tgaagcaaca cttctaaaat ttgtaaatga agcattagac aataacgaac aatcagttga
     3241 agattacaaa aatggtaaag gcaaagctat gggcttctta gttggtcaaa ttatgaaagc
     3301 gtctaaaggt caagctaatc cacaattagt aaatcaacta ttaaaacaag aattagataa
     3361 aagataattt aaatcatcaa actatgaaga tttaaaaaat aaacccttga ttgctgactt
     3421 agatgcaatc gagggtttat ttatatctat agaagtcaaa ttacttttaa ctttattcat
     3481 tgtacatgtt aatggtaaaa atattaattt tattaatgcg ttagctttaa tta
//
LOCUS       DALAAR010000145         3444 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000145 DALAAR010000000
VERSION     DALAAR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.151"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            227..337
                     /locus_tag="O2458_002712"
     CDS             227..337
                     /locus_tag="O2458_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888543.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            765..950
                     /locus_tag="O2458_002713"
     CDS             765..950
                     /locus_tag="O2458_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888544.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            1462..1566
                     /locus_tag="O2458_002714"
     CDS             1462..1566
                     /locus_tag="O2458_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888545.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            1695..1946
                     /locus_tag="O2458_002715"
     CDS             1695..1946
                     /locus_tag="O2458_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888546.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            2022..2684
                     /locus_tag="O2458_002716"
                     /pseudo
     CDS             2022..2684
                     /locus_tag="O2458_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            2697..2945
                     /locus_tag="O2458_002717"
     CDS             2697..2945
                     /locus_tag="O2458_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888547.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
     gene            2980..3102
                     /locus_tag="O2458_002718"
     CDS             2980..3102
                     /locus_tag="O2458_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888548.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            3078..3176
                     /locus_tag="O2458_002719"
                     /pseudo
     CDS             3078..3176
                     /locus_tag="O2458_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
BASE COUNT         1426 a          363 c          558 g         1097 t
ORIGIN      
        1 aacaaaatta atttttcgag tgtaaagaat cgtgtggtaa acataattta tatagattat
       61 aaggaggtga aaatggtcga ggaagttaaa acgataaaat agtttgaatg ttttgatttc
      121 aattctggta ggactattga atttgcaatc taataagaat gtgaggtttg atttaatatg
      181 aagtcatatt ctttgaataa aatattactt tctcaccatt agtaaaatga agatgactat
      241 taaagtgcga aaaacaaata aaaaagagaa aatagaattt ttaataggga cgtttataat
      301 tattttagta atattagggt tcaaaattat gaaataaatg tatagtattt gagaagtcta
      361 tatgactccg gaaaaattaa attctaaaga tatctcaata tctcaaattt aatagactac
      421 tggagttagt agtttgaata ttgaaagaca ctaatctaat aaaaatacat aaaaggttaa
      481 actttagaag tggctgtaaa cttcaatgaa tactaaacat tgaagttttg taaaaggcgt
      541 aaggtataca agacaaatcg tttatctttg acaaagtaat tgatgatgaa aagtcgttaa
      601 tatgtagaaa caaaaattga aaatataaaa tttaactatt atgaagtgat gatagccatt
      661 aatgaataga aaaataataa aatcatttat agataaaaaa tacgttaagt tcacagaatt
      721 tcgaaaaaaa gcagaaaaca ttgtacagat atggagatga aaaaatgtca gaatacaaga
      781 aaaaaataat tgaattaatt gaaagtaatt taacaggata tgaaatttct aaaaaaactg
      841 gagtttctca atacgtactt tcacaattaa gacagggcaa acgcgaagta gataatctaa
      901 ccctgaatac aacagaaaaa ttatatgaat atgccaataa agttttgtaa tttaactaat
      961 gtataaatta atcaagctat gtttatttga tttaactatt aataaaaatc atatggtgaa
     1021 tggatattat aataattaaa atacaaaaat agtagattcc aatttgtgaa aaaggagtat
     1081 gtcgtttcat ttagaattta ttgcacgaaa ttagcatcga ataagggaaa ataagtgtta
     1141 agttttaaat gataaaaaag attggaatgg atcgtcttga aatgctccct tcaaagtttt
     1201 cattttttca atgtcgactt cgaagggggc attttcatta aattgttata gctttttata
     1261 tttgtataat gaacatataa gtttaagaag gtgcgagtga aggaaataaa aaagctcaaa
     1321 tgtaccaaat tgttaatctt aataaatctc tactttataa agattgaatg gacattcgag
     1381 cgttaatcag tcaggaggga ctttccctcc tacaatttaa taataatact tgcttcacca
     1441 ctatacaagg agtgagttgt tatgttcaaa gtgaattatt cgattttaag ttattatcca
     1501 gaatataata tcgcagtaag ttggcaacgt ttaagagaag gaaaaacaat aaaaaacaag
     1561 atttaatact gctgcgtcat gaggcgcttg aacattattt gatgaataag tataatttca
     1621 actatgatta tgcacataaa attgtatcaa aaaaatacga ttattcaatt tttataaaaa
     1681 agaaggtgga ttaaatgctt acattaataa aattgaaaga agatgaacag gttataatat
     1741 atgaatatat acctgaagat gatataagta acggtaaagg ttcagtaact tttaataaaa
     1801 aagatgcaga ggttatagat ttctcattat ctgaaataga aaatgaagaa tattttatgt
     1861 tatatcgtaa taagtctttt tctgtagtaa gagactttat cgagaagcaa gaatttcccg
     1921 aaaattataa aatagcatgg tattaaagca tcctttctac acagataaag agaaagcgat
     1981 gctattttta tatgcttttt tcattattcg ttgaaaaaga agagtcacaa aagttaacat
     2041 atttaaaaga acttggtgaa gatggcgaat ataaatatgt tgccaaaata gatagtaaaa
     2101 catctaaatt atgtcattca ctcaacggaa aaatatttaa agttaaagat atgataccag
     2161 gtgtgaatgc gccacctatg catccttggt gtagaagtac cacagtgcca catgtcggca
     2221 attggcgaga caagttcttt aaagagcgtg aaggtaaata tcaagtagaa ggttgtttta
     2281 ttgaatcagg tgcacttaat aataaaagtg atgaatacgg tatcaaaagg aacagacatg
     2341 ctcaaattta ttataattca gttagaaatc gagataaaca gatagagatt tctaaaattg
     2401 ctaaaaatac taatataaat aaaaatttaa ttcaaagagt ttatgaacat atatttgaaa
     2461 ataagtattt attagaaagt ggatttaaac aatttgatcc tgacttttat atggctcaaa
     2521 gttggcaacg tttaagagaa gggaaaaata ttaaaaaaat ggatataata atgtagaaac
     2581 atgaagctct tgaacactat ctaatgaata agtataattt acactatata gaggcacata
     2641 aacttacaga aataaaatat aattatagta ttttaattaa ttagaggtgg aaatgattgc
     2701 taatattaaa aataaaagaa attaatgata aaagtgttac atataaatat tttcctaata
     2761 atgatgaaaa tattaagcct ggtattattc aaatggatat agatagtctt gaagttatca
     2821 atgctgaaaa atctagttta gaaaaaaata caagagataa ttattttatt catgccatag
     2881 acagaatata cataaatact agtaaagggc tatttcctga atctgaactc gtagcatggg
     2941 gataaagaaa gtttacttta aaaattttaa ccttcaaatg tgaaggttat tttttattgc
     3001 ccaaaacgtg ctgatgacat tttaaaagca agtatggaat atcagtcgac agactataaa
     3061 cggaggtata tctcatggaa aacaatgata gcaatgaagt agcttatttg ttaaaagatg
     3121 gtaaatttac aaaagtatat gtcaatcaag atagtgtatc ttttgtgcca gggtaacaag
     3181 caaccgaatt attatttaac agtaaaccga attcaattgt tatgttacat aaccatcctg
     3241 gacagtcagg attttcagaa tatgatttat ttacattttt taagcatcca tcaataaaat
     3301 caatgacaat cgttactaat aagggacaag taaaatttat taccaagtca aataggtttc
     3361 atggtaaaat agtgagtaaa ttttgtgcta aatattttac gcatattaat atcattaatg
     3421 atagccatat tgaaaaatta ttga
//
LOCUS       DALAAR010000146         3380 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000146 DALAAR010000000
VERSION     DALAAR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.153"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..352
                     /gene="lpl4"
                     /locus_tag="O2458_002720"
     CDS             <1..352
                     /gene="lpl4"
                     /locus_tag="O2458_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ3888549.1"
                     /translation="KLKQKIENFKFFSQYANLKELKNYSNGDVSINENVPSYDVKYKM
                     SNKDENVKQLRSRYNIPTDKSPVLKMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVID
                     SLDYQPAKTNKDDE"
     gene            371..1141
                     /gene="lpl5"
                     /locus_tag="O2458_002721"
     CDS             371..1141
                     /gene="lpl5"
                     /locus_tag="O2458_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCZ3888550.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDTNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            1173..1964
                     /gene="lpl6"
                     /locus_tag="O2458_002722"
     CDS             1173..1964
                     /gene="lpl6"
                     /locus_tag="O2458_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCZ3888551.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            2034..2810
                     /gene="lpl7"
                     /locus_tag="O2458_002723"
     CDS             2034..2810
                     /gene="lpl7"
                     /locus_tag="O2458_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCZ3888552.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
     gene            2989..>3380
                     /gene="lpl8"
                     /locus_tag="O2458_002724"
     CDS             2989..>3380
                     /gene="lpl8"
                     /locus_tag="O2458_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ3888553.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT         1448 a          339 c          640 g          953 t
ORIGIN      
        1 aaagcttaaa caaaaaatag aaaattttaa atttttcagt caatatgcaa atcttaaaga
       61 attgaaaaac tacagcaatg gtgatgtctc aattaatgag aatgttccaa gttatgacgt
      121 gaaatataaa atgagtaata aagatgaaaa tgttaagcaa ttaagaagtc gttataatat
      181 tcctactgat aaatcaccgg tattaaaaat gcatattgat ggtaatttga aaggaagttc
      241 cgttggagat aggaagttag aaattgattt ttcgaaacgt gaaaatagcc atttatcagt
      301 aatagattct ttggattacc agccagcgaa aactaataaa gatgatgaat aatgaggatg
      361 gtgtgtaaca atgaagtcta taaaaaggat tggattgtgc ataagtctgt tgattttaag
      421 catttttgta acatcttgcg atggtgataa taagatcact ggagattcaa aagaaacaca
      481 aatcaaaaag agcttttcga aaacgttaga tatgtatcca attaagaatc tcgaggactt
      541 atatgataaa gaaggatacc gtgatggcga atttaaaaag ggcgacaaag gaatgtggac
      601 tatatataca gattttgcta aaggcaataa atcagacgaa ttggatgatg aaggtatggt
      661 tttaaatctg gatagaaata ctcgaacggc taagggatat tattttgtta agaaatttta
      721 tgaaaaggat aaattacctg atagaaaaaa ttataaagtt gaaatgaaaa ataataaaat
      781 tatcttatta gataaagtag aagatacaaa tctaaaaaag agaatagaaa actttaaatt
      841 tttcggacaa tatgcaaatt ttaaggattt ggaaaattac aacaatggcg acgtgtcaat
      901 aaattggaat gttccaagtt atgacgtgga atataaaatg agcaataaag atgaaaatgt
      961 taaacaatta agaagtcgtt ataacattcc tactgataaa gctccaatgt taaaaatgca
     1021 tattgacggg gacttaaaag gtagttctgt tggatataaa aggttagaaa tagatttttc
     1081 aaaagaaggt agggatattt cagtcattga ttatttaagt tataagccag cgaaaaaata
     1141 gtactgataa ttaaatatta gggtgtgaag gaatggaata tctaaaaagg cttgcattgt
     1201 taataagtgt tattattttg accattttta taatgggttg tgatagtcaa agcgatactg
     1261 cagaaaatcc aaaagaaggt tcaaaagaag cacaaattaa aaagagtttt tcgaaaacgt
     1321 tagatatgta tccaattaag aatctcgagg atttttatgg caaagaagga tatcgagatg
     1381 gcgaatttaa aaaagatgat aaaggtactt ggctaattag atccgaaata gttaaacagc
     1441 caaagggcaa agtgatgaaa acaagaggta tgcaattata tattaataga aataccgaaa
     1501 cagccaaagg tttctttgtt ttgaaagaaa taagtgaaaa taataatcgt gtaaataaag
     1561 ataaggagga aaaatacgaa gtgaaaatgg taggaaataa aattattcct actgaacaaa
     1621 ttaatgacga gaaaataaaa aaagaaattg aaaacttcaa gttttttgtg caatatggaa
     1681 actttaaaaa tttcgaaaaa tacaacaatg gtgagttttc atataatcct gaagcaccaa
     1741 tttattcggc taaatatcaa ttacacaacg atgattacaa tgtaaggcaa ctacgtaaaa
     1801 gatatgacat ttcaacaaaa gaaacaccga agttactttt gaaaggtgga ggagatttaa
     1861 aaaattcctc agttggtcaa aacgatattg aatttacttt tgttgaaaga aaaggtgaga
     1921 atatttattt taacgatagt gttgaattca taccaagtaa gtaaagttaa attaaaatgt
     1981 gtggttagat gaaatgatca aatatcaaaa aatgtgcaaa gagatgtgac aagatgaagt
     2041 gttttcagaa attatacata tttatattaa ttttaatcgt attaatggca ggatgcgaaa
     2101 gtaataagat cactggagat tcgaaagaaa cacagatcaa aaagagcttt gcgaaaacat
     2161 tagatgtata ccctacgaaa aatctagaag atttttatga caaagaagga tatcgagatg
     2221 gcgaatttaa aaagggtgac aaagggaagt gggttattag atctgaaatg acaacagaac
     2281 tgaaaaatga aaatatggta tctaaaggta tggtcatacg tttaaataga aatagtagaa
     2341 catgcactgg tgaatatttt gtcaggatag ttaaagaaga cagtgagggc aaggtatata
     2401 gtgatgaacg aaaatatcca gtgaaaatgg aaaataataa aatcattaca ttaaaaccaa
     2461 tcgatgatga aaaagtaaaa aaagaaattg aagaatttaa attcttcgta caatacggga
     2521 atttcaaaga attggaaaac tataaagacg gagaagtgac atataaccca gaagcaccaa
     2581 tatactctgc acaatatcaa ttgaaaaata gtgattataa tgtagaacaa ctacgtaagc
     2641 gatataatat aacgacgaaa aaagcgccta aattattatt gaagggttca ggtaatttaa
     2701 aaggctcatc agttggatat aaaaatattg aatttacctt tgttgaaaat aaggaagaaa
     2761 atatttactt cacagatagt attaatttca acccaagtga ggataaataa gtatatttag
     2821 tattacaaaa acgttagaaa aattaataat aaagttaaat taacaaactg tattaaattc
     2881 atatagtcag ttttgtttga tgacatgata gtagaaaaat tattatttta ataggttgta
     2941 tgaactatac aaatatttat aaaaattaaa acagagagat gtgaaatgat ggggtatata
     3001 aaaagaatgg ctttatacat gagtgtattt cttttaatca tttttattgt tggatgtcga
     3061 aatatgaaag atgaacagaa aaaagaagaa caaacaaata aaacagattc aaaagaagaa
     3121 caaatcaaaa agagttttga gaaaacatta gatatgtatc caattaagaa tctcgaggag
     3181 ttatacgaca aagaaggata ccgagatggc gaatttaaaa agggtgataa agggatgtgg
     3241 acgatatata cagatttcgc caaaagtaat aaacaaggtg gattgagtaa tgaaggtatg
     3301 gtcttatact tagatagaaa tacacggact gcaaagggac attattttgt taagacattc
     3361 tataataagg gcaaattccc
//
LOCUS       DALAAR010000147         3352 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000147 DALAAR010000000
VERSION     DALAAR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.154"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            240..965
                     /locus_tag="O2458_002725"
     CDS             240..965
                     /locus_tag="O2458_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCZ3888554.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            1060..1785
                     /locus_tag="O2458_002726"
     CDS             1060..1785
                     /locus_tag="O2458_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCZ3888555.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1893..2609
                     /locus_tag="O2458_002727"
     CDS             1893..2609
                     /locus_tag="O2458_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCZ3888556.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(3054..3278)
                     /locus_tag="O2458_002728"
     CDS             complement(3054..3278)
                     /locus_tag="O2458_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888557.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(3235..>3352)
                     /locus_tag="O2458_002729"
     CDS             complement(3235..>3352)
                     /locus_tag="O2458_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888558.1"
                     /translation="IIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKNDRVE"
BASE COUNT         1256 a          509 c          485 g         1102 t
ORIGIN      
        1 attatcaaac aatttaatac aattgattag agaataattc taacgtatcg tttttaatat
       61 ataactcccg cctactttat tagatactgt ttctgtccaa aacttaaaaa tgataagttt
      121 tgcttaaata acactactaa ctgtttaagt ttatttaaca tagttttagc ttttatttaa
      181 ttccgaatcg gtgtaatagc ttatatactt tgggtaattc acgcaaagga gattttcata
      241 tgaaaaagaa catcatgaat aaattagttt tatcaacagc attgttactt ttagaaacta
      301 catcaacaca acttcctaaa acaccaatca gtttttcatc tgaagcaaaa gcctataata
      361 tcagtgaaaa cgagactaat atcaatgaac taatcaaata ttacactcag ccgcattttt
      421 cattatctgg aaaatggtta tggcaaaagc ccaatggtag cattcatgca acattgcaaa
      481 cgtgggtttg gtatagtcat attcaagtgt ttggatccga gagttgggga aacattaatc
      541 agttaagaaa taaatacgtt gatatatttg gaactaaaga tgaggacaca gttgaaggtt
      601 actggactta tgatgaaaca tttactggtg gtgttacgcc agcagctact tcatctgata
      661 agccttatag actattttta aaatatagtg ataaacaaca aactatcatc ggtggacatg
      721 aattttacaa aggaaataaa ccagtattaa ctttaaaaga attagatttc cgtattcgtc
      781 aaacattaat aaaaaataaa aagttatata acggagaatt taataaaggt caaattaaga
      841 taactgctga tggaaataat tacacgattg atttaagtaa aaagttaaaa ttaactgaca
      901 caaaccgtta tgttaaaaat cctcgtaatg cagaaattga agtcatactc gaaaaatcta
      961 actaacctat taccttttgt aaatgcggat aatttcaatt atctaattaa ccccttttta
     1021 taattaaaca ttccaacaat actcaaagga gaaattcgaa tgaacaataa catcacgaaa
     1081 aaaattattt tatcaacaac attgttacta ttaggtacag catctacaca atttcctaat
     1141 acacctatca attcttcatc tgaagcgaaa gcttattata taaatcaaaa cgaaactaac
     1201 gttaatgagt taactaaata ttactcgcaa aaatatttaa ccttctctaa cagtacgtta
     1261 tggcaaaaag ataacggtac gattcatgca acgttgttac agttttcttg gtatagtcat
     1321 attcaagttt atggacctga aagttggggc aatatcaacc aattaagaaa taaaagcgtt
     1381 gatatttttg gcataaaaga ccaagaaacc attgattctt ttgcattatc tcaagaaacg
     1441 tttactggtg gtgttactcc tgcagcaaca tctaacgata aacactataa actgaatgta
     1501 acatataaag ataaagcaga aacgtttact ggcggatttc cagtttatga aggcaataag
     1561 cctgttttaa ctttaaaaga attagatttt cgtattcgtc aaacattaat taaaagtaaa
     1621 aaattatata ataattctta taataaagga caaattaaaa taacaggtgc agacaataac
     1681 tacacaatag atttaagtaa aaggttgcca tcaactgatg caaatagata tgttaaaaaa
     1741 cctcaaaatg caaaaattga agttatcctc gaaaaatcaa actaacaata ataatggagt
     1801 taataaaaat aatcgcaaat actatattga cttcgctcac atttaaattt cttattcctc
     1861 gtatcatgat tcctctgaaa ggagatgttc taatgagtaa gaacatcacg aaaaatataa
     1921 ttttaacgac aacattatta ctattaggta ctgtattacc tcaaaatcaa aaaccagtat
     1981 ttagttttta ctctgaagct aaagcttata gcattggtca agatgaaact aacatcaatg
     2041 aattaattaa atattacaca cagcctcatt tttcattttc aaataaatgg ctatatcaat
     2101 atgataatgg aaacatttat gttgaactta agagatattc atggtcagca catatatctt
     2161 tatggggcgc tgaaagttgg ggaaatatta atcagttaaa agatcgttac gtagatgtgt
     2221 ttggactaaa agacaaagat actgatcagt tatggtggtc ttatagagag acatttacag
     2281 gtggcgttac accagccgca aaaccttctg ataaaactta taatcttttt gtgcaataca
     2341 aagataaact acaaacgatt attggtgcgc ataaaatata ccaaggcaat aaaccagtat
     2401 taacattgaa agaaatcgat ttccgtgcac gagaagcgtt aataaaaaat aaaatattat
     2461 ataacgaaaa tcgtaataaa ggtaagctta agatcaccgg tggcggtaat aactacacta
     2521 ttgatttaag caaaagatta cattcagatc tagcaaatgt ttatgttaaa aatcctaata
     2581 aaataactgt tgacgtcctc tttgattagt atatgaaggt gacttatact tcatgcactt
     2641 taattccaaa tcagattatt taaatgataa tttttaaagt gtatgatgta tataataggt
     2701 aaaattttct atatatttaa atggaattgg gagtaggaat gtgacagaaa tagtatttta
     2761 taaaatttat ttcgttgtca ctccccaact tgcacattat tgtaagctga cttttcgtca
     2821 gcttctgtgt tggggcccac cccaacttgc attgtctgta gaatttcttt ttgaaattct
     2881 ctatgttggg gccccgccta taattgaaaa atgcttgtta catgggcatt ttcattcggt
     2941 caactactac caatataata ttgtagagcc taagacattg atttattatg tcttaggctc
     3001 tattccttca tttaatgatt aaattattat agcaatactt tattgtccca tgattagtgt
     3061 tcttttaatg agacatagta actataaagt ttaataatcg ttctaaatct agcatcttct
     3121 agtttggttt tcccatttct taaatcttgt actgtttgat atggaactcc tgaatttttc
     3181 gaaattttat agcctgattc agactcaaac aaagattgaa gagagtttat aatattattc
     3241 aactctgtca tttttaatcc cctttcttga attaacaata tacaatgttg ttattaaaac
     3301 agtcagtgta tggatgattt catttcctaa aaatatattc agccatataa ta
//
LOCUS       DALAAR010000148         3332 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000148 DALAAR010000000
VERSION     DALAAR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.155"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(1..919)
                     /locus_tag="O2458_002730"
                     /pseudo
     CDS             complement(1..919)
                     /locus_tag="O2458_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            951..1120
                     /locus_tag="O2458_002731"
                     /pseudo
     CDS             951..1120
                     /locus_tag="O2458_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            1727..2077
                     /locus_tag="O2458_002732"
     CDS             1727..2077
                     /locus_tag="O2458_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888559.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            2164..2475
                     /locus_tag="O2458_002733"
     CDS             2164..2475
                     /locus_tag="O2458_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888560.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            2491..2994
                     /locus_tag="O2458_002734"
     CDS             2491..2994
                     /locus_tag="O2458_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCZ3888561.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            3008..3232
                     /locus_tag="O2458_002735"
     CDS             3008..3232
                     /locus_tag="O2458_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888562.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
BASE COUNT         1164 a          561 c          510 g         1097 t
ORIGIN      
        1 gtttttaatg tattttcgac aatatctatt acaatatgat tagagttatt aagctgatta
       61 ttgatgtaaa aagtttcaga ctttagcgag gaatggaaac tttctatcgg ggcgttatcg
      121 gcaggtgttc cctttcggga catacttctg ataatgcctt tttcttcgca taattgataa
      181 taagcataag atgtataaac gctgccttga tcactatgta atatacaccc ctcaggtata
      241 tcgatttgat ttaatgtatc attaactaaa cgttggtctt gtttatcatc tattttatac
      301 gccacaattt ctccgttata aatgtccatt atcgaagata aatacaacat agaatgacca
      361 aatggtaaat aagtaatatc ggttgttaat acttccattg gacaactcgc tttaaattgt
      421 ctttgtaata aattgtccgt tttataatac ggtttaccta tttttgtcat ctttttaggt
      481 ctaactcggc agttcaaatg atgcttctgc atcattctct gtactctctt atgattaatt
      541 ggtgatgtat aacattgatt aatcaatgct gtaatcttac gataaccgta ggtatagtgg
      601 ttagctttac ataattcaat aactttttgt gttacggtat catttttatg ggttttgttt
      661 ttccatcggt aatatgttga tttaggtatg tttaatactt ttagtagcaa tttgattgaa
      721 tattttactt ttaattgatc cactaaatct atgactactg ttggtaccac ttcctttcca
      781 ataccttgta cttttttaaa atatccaatt ctatatcttt tctcttattt tctaatttta
      841 attgttctac ttctgacagc tcttctaatc ctttaccgta ggtatattgt ttaccaactt
      901 gttgtgaaaa tctatatgct agttttggta aaactgagga aggacgctgg atagctaaga
      961 atgcccataa ttatggattt attataagat atcctaaaaa caaagaaagc atcacaggat
     1021 atcaatatga accttggcat ttacgttatt taggaaaaag aaggcgacaa aagtgtacaa
     1081 atctggtgaa acattagagg gatttgtagg cttaaaatga tcaatttaaa ataatcgaaa
     1141 cttcaactac cttattatcg acgaagtgta ttccagcact tcgtctttat ttttaccgca
     1201 aacattatac cttactattt aataaatcta ttttatatct acatacttcc tctttctttt
     1261 tctaaaaatt tttgagaatt ctctttatca atttcaataa cacttatcta atgaaacagt
     1321 cattgtcgta attttttcat aactacacga ggcttatatt gaaattgcta aagaacatcc
     1381 cacgatgatt ttttatcggg aaaggaatgg gatttgcaga ttagaagaag cagaaggatt
     1441 gtgcaagcac aatcccaaaa tcccgttatt ctttataaat aaataatata tataaggaag
     1501 cagagacccc accctatcat caatacttat aaataatgag ttgattgttc gtcaaaatag
     1561 gcggaatttc gggatttcct tgtcatacca aggtttgtag cggtttttta tatggaattt
     1621 tctcatattg tcaacgggat cagtaataaa tgacaaatca aatatatatt atctcaatga
     1681 tgtatatgaa gtacatcact atcacactat ctagggaggc aatgatatga aaacaactac
     1741 tcaagaactc aaacaatata tgactcgctt attccaatta tctaacaatg aaacatggga
     1801 atgtgaaaca ctggaggaag cggcagaaaa tattctaccc aaacgtttta ttaatgattc
     1861 cccacttgca catcttatac ttgaaactta tacctactat aataatgagc tacatgaact
     1921 aagtatctat ccatttctaa tgtattctaa taaccaactc atcagtattg gttatctgga
     1981 tcattttgat atggactttc tatacctcac agatactaaa aatacgatta tcgatgaacg
     2041 tcatttacta aaagaaggag ggaataacca tgaataattg gatcaaagtg gcacaaatat
     2101 ctgttacagt cattaacgaa gtgattgaca tcatgaaaga aaagcaaaat ggaggaaaat
     2161 agtatgaaaa tcaatcgata catcacaaga ggcattagtg aacaactatc tctagacctt
     2221 caaatcttac tttggaacat ggtaaaagat cgagacaatc aacttaatac agattaccta
     2281 cacattttca aactacaaga agatgataat atgttgtcaa ttacacatga acaagaacaa
     2341 cccgcataca aattggaata tcactataca aactatataa aaaatcaaaa tgcattacct
     2401 aagaaagtct acgtcatccg agaagatgat gtagacgctt tttattatgt gatgcttttg
     2461 ccagaagaat actaaaggaa gtgacgatat atgaatacaa tcaaaagtac gatacacaca
     2521 gaagcgatat ttagcgatga tgaacaacat cgctacttac tcaaaaagat atgggatgac
     2581 aagaaaccgg cttgtactgt gataacgatg tatcttcatt tagatggtgt attatcactc
     2641 gatcttacta cggttctcat cctcaatcaa ttagctaatt ctgagcaata tggcgctgtt
     2701 tatctcgtta atcttttctc taatattaaa acaccagaga accttaaaca tatcaaagag
     2761 ccttatgatg agcacacaga tatacactta atgaaagcaa ttagtgaaag tgacacagtc
     2821 attcttgctt atggtgccta tgcgaagcga ccagttgtta tcgaccgtgt cgaacaagtg
     2881 atggaaatgt taaaacctca taaaaagaaa gtaaaaaagc tcataaatcc agtaacgaac
     2941 gaagttatgc atccactcaa ccctaaagca cgtcaaaaat ggacactaaa gtaaaggagt
     3001 attatctatg aaccatgaca acactataca atccaattgg cgaacggttg ctagttgttt
     3061 agcatcacaa aattacattt caatagtaaa aggtctggtg catcatttca cagccatcga
     3121 tgacgaggaa atacttgata aaatctatga agagtttata aatgatgact ctattacaac
     3181 ggtacttaac aatgatttac aaacaattat taatcattat ctatcaaaat gaaaactata
     3241 ttctaataaa actagagcat ccttcacttt tgcggtgaag gatgctccta tatctttttt
     3301 tatctaaaaa aactattagt aattagtttt aa
//
LOCUS       DALAAR010000149         3233 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000149 DALAAR010000000
VERSION     DALAAR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.156"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            121..1407
                     /gene="rseP"
                     /locus_tag="O2458_002736"
     CDS             121..1407
                     /gene="rseP"
                     /locus_tag="O2458_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCZ3888563.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1427..3130
                     /locus_tag="O2458_002737"
     CDS             1427..3130
                     /locus_tag="O2458_002737"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCZ3888564.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
BASE COUNT         1117 a          438 c          645 g         1033 t
ORIGIN      
        1 ctttgaaaat tagacgttat attttgtgat ttgtcagtat catattataa tgacttatgt
       61 tacgtataca gcaatcattt ttaaaataaa agaaatttat aaacaatcga ggtgtagcga
      121 gtgagctatt tagttacaat aattgcattt attattgttt ttggtgtact agtaactgtt
      181 catgaatatg gccatatgtt ttttgcgaaa agagcaggca ttatgtgtcc agaatttgcg
      241 atcggtatgg ggccaaaaat ttttagtttt agaaaaaatg aaacacttta cactattagg
      301 ttattgcctg ttggtggata tgttcgtatg gcaggagatg gcttagaaga gccaccagtc
      361 gagcccggta tgaacgttaa aattaaactt aatgaagaaa atgaaataac acatatcata
      421 ttagatgatc atcataagtt tcaacaaatt gaagcgatcg aagttaaaaa atgtgatttt
      481 aaggatgact tattcataga aggtatcact gcttatgata atgaaagaca tcattttaaa
      541 attgctagaa agtctttctt tgttgaaaat ggtagcttag ttcaaattgc tccgagagac
      601 agacaatttg cacataaaaa gccatggccg aaatttttaa cattatttgc gggaccgtta
      661 tttaacttta tattagcttt agtcctattt attggtcttg catattatca aggcacgcct
      721 acgtctactg tagaacaagt cgcagataag tatccagctc aacaagcagg attacaaaaa
      781 ggtgataaga tcgtccaaat tggcaaatat aaaatatctg aatttgatga tgttgataag
      841 gcgttagata aagttaaaga taataagacg actgttaaat ttgaacgtga tggtaaaaca
      901 aagtcagttg aattaacacc taaaaagact gaaaaaaaac tgactaaagt aagttcagag
      961 acgaagtatg ttctcggatt ccaaccagcg agtgaacata cactttttaa accaattgta
     1021 ttcggattta aaagcttttt aatcggtagt acttatattt ttacagctgt agtaggtatg
     1081 ttggctagta tatttacggg cggattctca tttgatatgt taaatggtcc ggttggtatt
     1141 tatcataacg tcgactcagt tgttaaagcg ggtatcatta gcttaattgg ttacactgcg
     1201 ttattaagtg taaacttagg tattatgaat ttaattccta ttcctgcact agacggtggt
     1261 cgtattttat ttgttatata tgaagcgatt ttcagaaaac cagttaataa aaaagcggaa
     1321 acaacgatta ttgctattgg tgccattttc atggtcgtta taatgatatt agtaacgtgg
     1381 aatgatattc gacgatattt cttataattt aggaggataa ataattatga agcaatccaa
     1441 agtttttata ccaacgatgc gtgacgtgcc atcagaagca gaagcacaaa gtcatcgttt
     1501 attattgaaa tcgggtttga taaaacaaag tacaagtggg atttatagtt atttaccgct
     1561 agcaacacgt gtgttaaata atattactgc aattgtgcga caagaaatgg aacgtatcga
     1621 ttctgttgaa attttaatgc cagcgttaca acaagctgaa ttatgggaag aatcaggacg
     1681 ttggggtgca tatggcccag aattaatgcg tttacaagat agacatggaa gacaatttgc
     1741 attaggtcca acacatgaag aattagttac atcaatagta agaaatgaat tgaaatcata
     1801 caaacaatta ccgatgacat tattccaaat tcaatctaaa ttccgtgatg aaaagagacc
     1861 acgttttggt ttacttcgtg ggcgtgaatt tattatgaaa gatgcatatt cattccatgc
     1921 tgacgaggca tcattagatc aaacgtatca agatatgtat caagcgtata gccgtatttt
     1981 tgagagagtt ggcattaacg caagaccagt agttgcagat tcaggtgcta taggcggtag
     2041 ccatacacat gaatttatgg cattaagtgc tatcggtgag gatacaatcg tttacagtaa
     2101 agaaagtgat tatgctgcta acatcgaaaa agcagaagtc gtttacgaac caaatcataa
     2161 gcatactact gtgcaacctt tagaaaaaat tgaaacacca aatgttaaga ctgcgcaaga
     2221 attggcagac ttcttaggta gaccagtaga tgaaatcgtt aaaacgatga ttttcaaagt
     2281 tgatggcgaa tatattatgg ttttagtgcg tggccatcat gaaattaatg acattaaatt
     2341 aaaatcttat ttcggcacag ataatattga attagcaaca caagacgaaa ttgttaattt
     2401 agttggtgca aatcctggtt cactaggtcc tgtaattgat aaagaaatca aaatttatgc
     2461 agataatttt gtgcaagatt taaataattt agttgtcggt gctaacgaag atggttatca
     2521 cttaattaat gtaaatgtag gtagagactt caacgttgat gaatatggcg atttccgttt
     2581 tattttagaa ggcgaaaagt taagtgatgg ttcaggcgtt gcacattttg ctgaaggtat
     2641 tgaagttggt caagtattca aattgggtac taagtattca gaatcaatga atgctacatt
     2701 cttagataac caaggaaaag ctcaatcttt aattatgggt tgttacggaa ttggaatttc
     2761 tagaacgcta agtgcgattg ttgaacaaaa tcacgatgat aatggaattg tttggcctaa
     2821 atcagttact ccgtttgatt tacatttaat ttctattaat cctaagaaag atgatcaacg
     2881 agaactagca gatgcactat atgctgaatt taatactaaa tttgatgtgt tgtacgatga
     2941 tcgtcaggaa cgtgcaggtg ttaaatttaa tgatgccgat ttaattggtt taccactgcg
     3001 aattgttgtt ggtaaacgtg catcggaagg tattgtagaa gttaaagaac gtttaacagg
     3061 tgatagcgaa gaagttcaca ttgatgactt aatgactgtc attacaaata aatatgataa
     3121 cttaaaataa ttaagatcga atgaattata agagtaggaa aaagctgaaa gaaatctgat
     3181 gcttatgtcc tgctcttatt atttttgata taatgattat tcgatgaaaa atg
//
LOCUS       DALAAR010000150         3222 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000150 DALAAR010000000
VERSION     DALAAR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.157"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            171..1214
                     /locus_tag="O2458_002738"
     CDS             171..1214
                     /locus_tag="O2458_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCZ3888565.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            1217..1897
                     /gene="sdaAB"
                     /locus_tag="O2458_002739"
     CDS             1217..1897
                     /gene="sdaAB"
                     /locus_tag="O2458_002739"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCZ3888566.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            1911..2810
                     /gene="sdaAA"
                     /locus_tag="O2458_002740"
     CDS             1911..2810
                     /gene="sdaAA"
                     /locus_tag="O2458_002740"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCZ3888567.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            2829..2927
                     /locus_tag="O2458_002741"
     CDS             2829..2927
                     /locus_tag="O2458_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888568.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            2989..3159
                     /locus_tag="O2458_002742"
     CDS             2989..3159
                     /locus_tag="O2458_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888569.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
BASE COUNT         1082 a          485 c          673 g          982 t
ORIGIN      
        1 acaacataag tagtttttga aacaatccat tttaaaaatc tttcacaaat atcaaatatt
       61 tcacttaaat atttgatttg ataacagata aaagttgaaa acgttttctt aagggtgtac
      121 gatgtttatg gtctgaaaat aaaataaaca tagagagagg tttttgggaa atggatattc
      181 tgttaggtgt aggcacatta gtcttggttt tagtgatcat gacgctattt ttaaagtatg
      241 caccgtacgg taaacaagga ctacaagcat tatccggtgc agcttgtgcg acatttctac
      301 cacaagcatt cttaagttat gctattggtg gcgtatttga tattaaattt ctacaagata
      361 ttggggattt agcaggaagt ttaagcggta tcgcagtagg tattctaacg tgcttaaact
      421 taggcgttgc accagtattt gcggtcattg ttggtttagt attacatgac tttaaattat
      481 taccagcatt catcgcagct tatggcgtag cttttttaat taaatggatt gaaaagaaag
      541 ttccagaagg cttggactta attgtggtga tcttatttgc accagcaatt gctttcggac
      601 ttgcatcaat cattacacct ggtgtgctag caacattaaa acaaataggt agtgctgtca
      661 ctgcggtagg tgataacaat ccatatgcat tagcggtcat tttaggacta gtgattcctg
      721 ttacaggtat gacaccgcta agttcaatgg tattaacaag tttattagga ctgacaggtg
      781 ttcctatggc tattggagca ctaacatgta caggtagctc atttgttaac cttattttat
      841 tccgaaaatt aaacattggt ggaccgtcaa aagcatttgc agtttgtatt gagccattga
      901 cgcaaattga tttaatagcg caatatccag tacaactata tggtacaaat gcactgattg
      961 gggtcgtcaa cgcatgtatc gtgacattta gcggactagt aataggtgtc aaaggtatgg
     1021 caacaccaat tgcaggcgcc atcgtattgt ttggctttaa taatgcagtg acatcaattg
     1081 taacaatcgc aacagtgatt attgtcagca ttgtcttagc ctatattatt ggaacactaa
     1141 ttaataagtt taatttaatg aatatcaact ttaaaatgcc gagcaaaaag aatcatatta
     1201 aggagagtgt ttaattatgg caaagagcta tgattatcaa agtgctttcg atattatcgg
     1261 accagttatg atgggccctt caagttctca tacggctggt gcagtaaaga ttgggaattc
     1321 agcacgcgca atattgggag atattcctaa aaatattgaa atacattatt atgaatcatt
     1381 tgctcaaaca caccaaggac atggtacaga tgtcgctatt gtgggtggag caatgggata
     1441 ttcaacattt gataaccgaa ttaaatcttc attagaaatt gcagctgatg atggtattaa
     1501 aatagatatc atcgaagaag aaggcgatag tattggtcaa catccaaact gtgcttatat
     1561 caaatcatct cgtaatgatg gtcgctacat tgaaattatc ggtatttcaa tcggtggtgg
     1621 tacaattaaa attaaaggta ttcatataaa tggactagaa gtagatttaa atcatggatt
     1681 accaatttta gttgttgacg gaaatatgac taaagcacaa gtaaatcatt tcatcaatga
     1741 tatcaacgat atgaaattag actgtaaaga tgaatttatt aagatagatg aagataaatg
     1801 cttggttgta ataccattaa ataaagcaat ctcagaatca acattaaata caattaaaga
     1861 gaaatacagt gacgtaaacg tttcctatat aaattagagg gggaataaac atgtttgatt
     1921 caattagaga gactatagat tatgccgtag aaaataatat gtcatttgcg gatatcatgg
     1981 ttaaagaaga aatggaatta agcggtaaat cacgtgatga agtgcgagcg caaatgaaac
     2041 aaaatttaga tgtcatgcga gacgcagtaa tcaaagggac gacaggtgat ggggttgaaa
     2101 gtgtaacggg ctacactggt catgatgctg ctaaactacg tgattataat gaaacacatc
     2161 atgctttgtc tggatatgaa atgattgacg cagtcaaagg tgccattgca acaaatgaag
     2221 tcaatgctgc gatgggtatt atttgtgcaa cgccaacagc tggttcctcg ggtaccattc
     2281 ccggtgcact ttttaaatta gaaaaaacac atgatttaac agaagagcaa atgattgatt
     2341 tcttattcac ttcagcattg tttgggcgtg tcgtagcaaa caatgcaagt gtagctggtg
     2401 caacaggtgg ctgccaagct gaagttggtt cagcatctgc aatggccgca gctgcagcag
     2461 tagctatatt cggaggatca ccagaagcat ccgggcacgc tatggcatta gcgataagta
     2521 atttattagg tttagtttgt gatccagtag ccggacttgt tgaaattcct tgtgttatga
     2581 gaaatgcaat tggttcgggt aacgctttaa tttcagcaga tttagcatta gcaggtattg
     2641 aaagtagaat ccctgttgat gaagttattg aagcaatgga taaggttggt cgtaaccttc
     2701 ctgcatcatt acgtgaaact ggattgggtg gactagcagg cacaccaact ggcgaagcaa
     2761 ttaaacgtaa aatctttggc acagctgaag atatggttaa aaataattaa tgagataaca
     2821 ataaaatatt gataaaaaag aagcatctgt ctggatcatt agtaccagat agatgcttct
     2881 taattatctc tgaagaagtt gaaagattta tggaaaatgc atactaatca aaatagtgat
     2941 tgcaaaagcg cccccgtata tctaaaataa cttaaaagga gtgatgatat gctaagacta
     3001 tcaatcatct ttatagcatt catcattaac acaacgatta catatggata tacgactgag
     3061 ggaacgtggg tcaatctatt atttaaatct ttatcgctca gcatgattat cgttttcatg
     3121 ttctactata ttcgttttgt tattgagaaa aagcgttaaa tcaaagctat ggggatagta
     3181 tttgaaaaga ataaataaaa gggacaatgt aaatcaatgt aa
//
LOCUS       DALAAR010000151         3169 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000151 DALAAR010000000
VERSION     DALAAR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.158"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            199..3024
                     /gene="sdrC"
                     /locus_tag="O2458_002743"
     CDS             199..3024
                     /gene="sdrC"
                     /locus_tag="O2458_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCZ3888570.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNS
                     NNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1276 a          501 c          554 g          838 t
ORIGIN      
        1 aagaaaaaaa gtattaaaaa ttgcaattct taaactgtga ttcatattaa tatttctagc
       61 aaataacatt gtaaaataaa gaaaaataat taaagtattg cactttattg aaatttatat
      121 tacgatagta atgcagaaat ttatatatgc aaaatattat atttatcaaa ttttgatatt
      181 ttaaaggagt attattaaat gaataataaa aagacagcaa caaatagaaa aggcatgata
      241 ccaaatcgat taaacaaatt ttcgataaga aagtattctg taggtactgc ttcaatttta
      301 gtagggacaa cattgatttt tgggttaagt ggtcatgaag ctaaagcggc agaacatacg
      361 aatggagaat taaatcaatc aaaaaatgaa acgacagccc caagtgagaa taaaacaact
      421 aaaaaagttg atagtcgtca actaaaagac aatacgcaaa ctgcaactgc agatcagcct
      481 aaagtgacaa tgagtgatag tgcaacagtt aaagaaacta gtagtaacat gcaatcacca
      541 caaaacgcta cagctaatca atctactaca aaaactagca atgtaacaac aaatgataaa
      601 tcatcaacta catatagtaa tgaaactgat aaaagtaatt taacacaagc aaaagatgtt
      661 tcaactacac ctaaaacaac gactattaaa ccaagaactt taaatcgcat ggcagtgaat
      721 actgttgcag ctccacaaca aggaacaaat gttaatgata aagtacattt ttcaaatatt
      781 gacattgcga ttgataaagg acatgttaat cagactactg gtaaaactga attttgggca
      841 acttcaagtg atgttttaaa attaaaagca aattacacaa tcgatgattc tgttaaagag
      901 ggcgatatat ttacttttaa atatggtcaa tatttccgtc caggatcagt aagattacct
      961 tcacaaactc aaaatttata taatgcccaa ggtaatatta ttgcaaaagg tatttatgat
     1021 agtacaacaa acacaacaac atatactttt acgaactatg tagatcaata tacaaatgtt
     1081 agaggtagct ttgaacaagt tgcatttgcg aaacgtaaaa atgcaacaac tgataaaaca
     1141 gcttataaaa tggaagtaac tttaggtaat gatacatata gcgaagaaat cattgtcgat
     1201 tatggtaata aaaaagcaca accgcttatt tcaagtacaa actatattaa caatgaagat
     1261 ttatcgcgta atatgactgc atatgtaaat caacctaaaa atacatatac taaacaaacg
     1321 tttgttacta atttaactgg atataaattt aatccaaatg caaaaaactt caaaatttac
     1381 gaagtgacag atcaaaatca atttgtggat agtttcaccc ctgatacttc aaaacttaaa
     1441 gatgttactg atcaattcga tgttatttat agtaatgata ataaaacagc tacagtcgat
     1501 ttaatgaaag gccaaacaag cagcaataaa caatacatca ttcaacaagt tgcttatcca
     1561 gataatagtt caacagataa tggaaaaatt gattatactt tagacactga caaaactaaa
     1621 tatagttggt caaatagtta ttcaaatgtg aatggctcat caactgctaa tggcgaccaa
     1681 aagaaatata atctaggtga ctatgtatgg gaagatacaa ataaagatgg taaacaagat
     1741 gccaatgaaa aagggattaa aggtgtttat gtcattctta aagatagtaa cggtaaagaa
     1801 ttagatcgta cgacaacaga tgaaaatggt aaatatcagt tcactggttt aagcaatgga
     1861 acttatagtg tagagttttc aacaccagcc ggttatacac cgacaactgc aaatgtaggt
     1921 acagatgatg ctgtagattc tgatggacta actacaacag gtgtcattaa agacgctgac
     1981 aacatgacat tagatagtgg attctacaaa acaccaaaat atagtttagg tgattatgtt
     2041 tggtacgaca gtaataaaga tggtaaacaa gattcgactg aaaaaggaat taaaggtgtt
     2101 aaagttactt tgcaaaacga aaaaggcgaa gtaattggta caactgaaac agatgaaaat
     2161 ggtaaatacc gctttgataa tttagatagt ggtaaataca aagttatctt tgaaaaacct
     2221 gctggcttaa ctcaaacagg tacaaataca actgaagatg ataaagatgc cgatggtggc
     2281 gaagttgatg taacaattac ggatcatgat gatttcacac ttgataatgg ctactacgaa
     2341 gaagaaacat cagatagcga ttcagactca gatagcgact cagattcaga tagcgactca
     2401 gattcagaca gcgattcaga cagcgactca gactcagata gcgattcaga ttcagacagc
     2461 gactcagact cagacagcga ttcagactcg gatagcgact cagactcaga tagcgactca
     2521 gactcggata gcgactcaga ctcagatagc gattcagatt cagatagcga ttcggactca
     2581 gacagtgatt cagattcaga ctcagatagc gactcagatt ctgacagcga ttcagactca
     2641 gacagcgact cagactcaga cagtgattca gattcagaca gcgactcaga ttcagatagc
     2701 gactcagact cagatagcga ctcagactca gatagcgact cagactcgga tagcgattca
     2761 gattcagaca gcgactcaga ttcagatagc gattcggact cagacaacga ctcagattca
     2821 gatagcgatt cagattcaga tgcaggtaaa catactccgg ctaaaccaat gagtacggtt
     2881 aaagatcagc ataaaacagc taaagcatta ccagaaacag gtagtgaaaa taataattca
     2941 aataatggca cattattcgg tggattattc gcggcattag gatcattatt gttattcggt
     3001 cgtcgtaaaa aacaaaataa ataatatgat taacttaacc aggtccatgt ggcctggttt
     3061 tttctgttta gaaattccca gatataaaac aaaaaatctt taaggaatag atacaattta
     3121 atttgttaca gaaatgtaat tgtctatgaa ttagaaaagc taagatatt
//
LOCUS       DALAAR010000152         3125 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000152 DALAAR010000000
VERSION     DALAAR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.159"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(55..243)
                     /locus_tag="O2458_002744"
     CDS             complement(55..243)
                     /locus_tag="O2458_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888571.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="O2458_002745"
     CDS             complement(826..1821)
                     /locus_tag="O2458_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCZ3888572.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="O2458_002746"
     CDS             2273..2854
                     /locus_tag="O2458_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888573.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DALAAR010000153         3125 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000153 DALAAR010000000
VERSION     DALAAR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.160"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            85..>3125
                     /locus_tag="O2458_002747"
     CDS             85..>3125
                     /locus_tag="O2458_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCZ3888574.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPK"
BASE COUNT         1315 a          442 c          531 g          837 t
ORIGIN      
        1 aaaaatgtaa tactatattg atatacattt ttgtatttaa aacaattgta ttatttaaaa
       61 atttgatgaa ttaggagtaa tctaatgcta aacagagaaa ataaaacggc aataacaagg
      121 aaaggcatgg tatccaatcg attaaataaa ttttcgatta gaaagtacac agtgggaaca
      181 gcatcaattt tagtaggtac aacattaatt tttggtctgg ggaaccaaga agcaaaggct
      241 gcagaaagta ctaataaaga attgaacgaa gcgacaactt cagcaagtga taatcaatcg
      301 agtgataaag ttgatatgca gcaactaaat caagaagaca atactaaaaa tgataatcaa
      361 aaagaaatgg tatcatctca aggtaatgaa acgacttcaa atgggaataa attaatagaa
      421 aaagaaagtg tacaatctac cactggaaat aaagttgaag tttcaactgc caaatcagat
      481 gagcaagctt caccaaaatc tacgaatgaa gatttaaaca ctaaacaaac tataagtaat
      541 caagaagcgt tacaacctga tttgcaagag aataaatcag tggtaaatgt tcaaccaact
      601 aatgaggaaa acaaaaaggt agatgccaaa actgaatcaa ctacattaaa tgttaaaagt
      661 gatgctatca agagtaatga tgaaactctt gttgataaca atagtaattc aaataatgaa
      721 aataatgcag atatcatttt gccaaaaagt acagcaccta aacgtttgaa tacaagaatg
      781 cgtatagcag cagtacagcc atcatcaaca gaggctaaaa atgttaatga tttaatcaca
      841 tcaaatacaa cattaactgt cgttgatgca gataaaaaca ataaaatcgt accagcccaa
      901 gattatttat cattaaaatc acaaattaca gttgatgaca aagttaaatc aggtgattat
      961 ttcacaatta aatactcaga tacagtacaa gtatatggat tgaatccgga agatattaaa
     1021 aatattggtg atattaaaga tccaaataat ggtgaaacaa ttgcgactgc aaaacatgat
     1081 actgcaaata atttaattac atatacattt acagattatg ttgatcgatt taattctgta
     1141 caaatgggaa ttaattattc aatttatatg gatgctgata caattcctgt tagtaaaaac
     1201 gatgttgagt ttaatgttac gataggtaat actacaacaa aaacaactgc taacattcaa
     1261 tatccagatt atgttgtaaa tgagaaaaat tcaattggat cagcgttcac tgaaacagtt
     1321 tcacatgttg gaaataaaga aaatccaggg tactataaac aaacgattta tgtaaatcca
     1381 tcggaaaatt ctttaacaaa tgccaaacta aaagttcaag cttaccactc aagttatcct
     1441 aataatatcg ggcaaataaa taaagatgta acagatataa aaatatatca agttcctaaa
     1501 ggttatacat taaataaagg atacgatgtg aatactaaag agcttacaga tgtaacaaat
     1561 caatacttgc agaaaattac atatggcgac aacaatagcg ctgttattga ttttggaaat
     1621 gcagattctg cttatgttgt aatggttaat acaaaattcc aatatacaaa tagcgaaagc
     1681 ccaacacttg ttcaaatggc tactttatct tcaacaggta ataaatccgt ttctactggc
     1741 aatgctttag gatttactaa taaccaaagt ggcggagctg gtcaagaagt atataaaatt
     1801 ggtaactacg tatgggaaga tactaataaa aacggtgttc aagaattagg agaaaaaggc
     1861 gttggcaatg taactgtaac tgtatttgat aataatacaa atacaaaagt aggagaagca
     1921 gttactaaag aagatgggtc atacttgatt ccaaacttac ctaatggaga ttaccgtgta
     1981 gaattttcaa acttaccaaa aggttatgaa gtaacccctt caaaacaagg taataacgaa
     2041 gaattagatt caaacggctt atcttcagtt attacagtta atggcaaaga taacttatct
     2101 gcagacttag gtatttacaa acctaaatac aacttaggtg actatgtctg ggaagataca
     2161 aataaaaatg gtatccaaga ccaagatgaa aaaggtatat ctggcgtaac ggtaacatta
     2221 aaagatgaaa acggtaacgt gttaaaaaca gttacaacag acgctgatgg caaatataaa
     2281 tttactgatt tagataatgg taattataaa gttgaattta ctacaccaga aggctataca
     2341 ccgactacag taacatctgg tagcgacatt gaaaaagact ctaatggttt aacaacaaca
     2401 ggtgttatta atggtgctga taacatgaca ttagatagtg gattctacaa aacaccaaaa
     2461 tataatttag gtaattatgt atgggaagat acaaataaag atggtaagca ggattcaact
     2521 gaaaaaggta tttcaggcgt aacagttaca ttgaaaaatg aaaacggtga agttttacaa
     2581 acaactaaaa cagataaaga tggtaaatat caatttactg gattagaaaa tggaacttat
     2641 aaagttgaat tcgaaacacc atcaggttac acaccaacac aagtaggttc aggaactgat
     2701 gaaggtatag attcaaatgg tacatcaaca acaggtgtca ttaaagataa agataacgat
     2761 actattgact ctggtttcta caaaccgact tacaacttag gtgactatgt atgggaagat
     2821 acaaataaaa acggtgttca agataaagat gaaaagggca tttcaggtgt aacagttacg
     2881 ttaaaagatg aaaacgacaa agttttaaaa acagttacaa cagatgaaaa tggtaaatat
     2941 caattcactg atttaaacaa tggaacttat aaagttgaat tcgagacacc atcaggttat
     3001 acaccaactt cagtaacttc tggaaatgat actgaaaaag attctaatgg tttaacaaca
     3061 acaggtgtca ttaaagatgc agataacatg acattagaca gtggtttcta taaaacacca
     3121 aaata
//
LOCUS       DALAAR010000154         3114 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000154 DALAAR010000000
VERSION     DALAAR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.161"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            327..827
                     /locus_tag="O2458_002748"
     CDS             327..827
                     /locus_tag="O2458_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888575.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            839..1339
                     /locus_tag="O2458_002749"
     CDS             839..1339
                     /locus_tag="O2458_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888576.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QDEAEL"
     gene            1350..1850
                     /locus_tag="O2458_002750"
     CDS             1350..1850
                     /locus_tag="O2458_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCZ3888577.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            1861..2361
                     /locus_tag="O2458_002751"
     CDS             1861..2361
                     /locus_tag="O2458_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888578.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            2372..2860
                     /locus_tag="O2458_002752"
     CDS             2372..2860
                     /locus_tag="O2458_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCZ3888579.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT         1232 a          298 c          622 g          962 t
ORIGIN      
        1 atttacgaaa tggcaaaaaa gtatatacta ttaatatctt ttttgctata attataaata
       61 gttaaataaa tagaaagaca aaataatgag taggagtttt agggctatta tttacaaata
      121 atataatact gcaaagactc gaaattattt agaattccag ttatcgtaac aatagttgca
      181 gttattttag ttccattagt gtatttaggt ttaaaatgag taatattaat ttgaagaagc
      241 attaagcgac gtagaggtgt tgtttagtgc tttttattta acggaaatga aaccaatatc
      301 tattttgaaa atataggggc gataacatga ctttcgaaga aaaactaagt gaaatataca
      361 acaagattgc gaatgagatt agtgggatga taccagtaga atgggaccaa gtatttacga
      421 tagcttatgt aaatgataga ggtggagaga tcgtttttaa ttatactaaa ccaggtagcg
      481 atgaattgaa ttattacaca tatatcccta gagagtataa tgtctctgaa aaagtatttt
      541 atgatttgtg gacggattta tatagattgt ttaagaagtt aagaaacgca tttaaagaag
      601 aagatttaga accatggaca tcatgtgaat ttgactttac aagagatggt aaattgaatg
      661 ttgtatttga ttatgttgat tggatgaatt cagaatttgg tccaatagca aaagaaaatt
      721 attatatgta caaaaagttt ggtgttttac cagaaacgga atatgaaatt aataaagtta
      781 aagaaatcga tcaatatgtt aaagagcaag atgaagctga actatagggg gagataacat
      841 gactttcgaa gagaaactaa gtcaaatgta caataaaatt gcaagtgaga tcagtggaat
      901 gataccagta gaatgggagc aagtatttac aatagcttat gtaactgatc aagctgggga
      961 agtcattttt aattatacta aaccaggtag tgatgaatta aattattatt cagacatacc
     1021 taaagattgc aatgtctcaa aagatatttt taagaattca tggtttaaag tttatcgaat
     1081 gtttgatgag ttaagagaaa cttttaaaga agaagagctt gaaccatgga catcatgcga
     1141 atttgacttt acaagagatg gcaaattgaa tgtatctttt gattatattg attgggtgaa
     1201 ttcagaattt ggaccaatgg gaagagaaca ttattatatg tataaaaaat ttggaatttg
     1261 gcctgaaaaa gaatatgcca taaattgggt tgaaaaaata aaagattatg ttaaagagca
     1321 agatgaagct gaactatagg ggagataata tgactttcga aggaaaacta agtcaaatgt
     1381 acaacgagat tgcgaatgag attagtggga tgataccggt agaatgggag caagtattta
     1441 caatagctta tgtaactgat caagctggag aagtcatctt taattatact aaaccagata
     1501 gtgatgaatt aaattattat tcagacatac ctaaagattg caatgtctca aaagatattt
     1561 ttaagaattc atggtttaaa gtttatcgaa tgtttgatga gttaagagaa acttttaaag
     1621 aagaagggct tgaaccatgg acatcatgcg aatttgactt tacaagagat ggcaaattga
     1681 atgtatcttt tgattatata gattggataa atacagagtt tgatcaattg ggccgtcaaa
     1741 attattatat gtacaaaaaa tttggggtta taccagaaat ggaatatgaa atggaagaag
     1801 ttaaagaaat cgaacaatat attaaagagc aagaagaagc tgaacaatag aggtgataac
     1861 atgactttcg aagagaaaat aagcaaatta tataatgaga ttgcgaatga gattagcagt
     1921 atgataccgg tagagtggga aaaagtatat acaatggctt atatagatga tggaggaggt
     1981 gaagtattct ttaattatac taaaccaggt agtgatgact tgaattatta caccgatata
     2041 cctaaggagt ataacatctc tgtgcaagta tttgatgatt tatggatgga tttatatgat
     2101 ttgtttgagg aattaagaga tttatttaaa gaagaaggac ttgaaccatg gacatcatgt
     2161 gaatttgact ttacaagcga aggtaaatta aaagtttcat ttgattatat agattggata
     2221 aatacagagt ttgatcaatt aggccgtgaa aattattata tgtataaaaa atttggggtt
     2281 ttaccagaaa tggaatatga aatggaagaa attaaagaaa tcgatcaata tattaaagag
     2341 caagatgaag ctgaaatata ggggagataa catgactttc gaagaaaagc taagtcaaat
     2401 gtacaatgaa attgcaaatg aaatcagtgg aatgatacca gtagaatggg aaaaagtata
     2461 tacaattgcc tacgtagatg atgaaggtgg agaggttgtt tttaattata ctaaaccagg
     2521 aagtgaagat ttgaattatt attcagatat tcctaaagat tgcaatgtct caaaagatat
     2581 ttttaagaat tcatggttta aagtttatcg aatgtttgat gagttaagag aaacttttaa
     2641 aaaagaagat ttagaaccgt ggacatcatg tgaatttgac tttacaagaa agggaaattt
     2701 aaaagtatca tttgattata tagattggat taaattaggt tttggcccat caggaaagga
     2761 aaactactat atgtacaaaa aatttggtat tttaccagat atggaatatg aaatggaaga
     2821 aattcgagca gtagagaagt atgttaaaga gcaagagtag cagacatgtt ataaaagact
     2881 gtgcaaaatc accctcgttt tacatttgat tcaaagaaga aggtaaaaga taagattatt
     2941 tgcaacttaa aaagtcaatt agcttatcgg tatccataca tcatggataa atgagttcaa
     3001 ctaattaaca aatcacgata taaatttaaa attgtcatta attgaaaagg ttattgtagt
     3061 gtattttata aagttgatgt acatcgactt attttttaaa acttatatca aaaa
//
LOCUS       DALAAR010000155         3096 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000155 DALAAR010000000
VERSION     DALAAR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.162"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            453..1388
                     /gene="lukE"
                     /locus_tag="O2458_002753"
     CDS             453..1388
                     /gene="lukE"
                     /locus_tag="O2458_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCZ3888580.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1390..2373
                     /gene="lukD"
                     /locus_tag="O2458_002754"
     CDS             1390..2373
                     /gene="lukD"
                     /locus_tag="O2458_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCZ3888581.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2615..2758
                     /locus_tag="O2458_002755"
     CDS             2615..2758
                     /locus_tag="O2458_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ3888582.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1183 a          404 c          519 g          990 t
ORIGIN      
        1 ttagactaaa tcatagcatt aaattttttt agatgaagta agcgatatta cgaaatgata
       61 tatagaacat ttcagttata acagtatacg aagtaatcat cttcgtttaa cggacaatag
      121 ttaataatca gttaatttta aattcaatta tagattattt gtgataatac aaacattatt
      181 tttgaagatt tgattaaagt cgtttttatt tttacagaga aaagattgag tatcggtaat
      241 tttatcatat tgttcgaata taaagggaac tatttaaagg taatgaaggt tgcgccaaaa
      301 tgaccggttt tcagcagcat aaatgaataa attgtgtcat ttttaatatt ttatagcatt
      361 ttatttttta atgaacagtt aattttataa ataagtgaaa taatctagaa taaatttgta
      421 ttaaaaattt atatagaaag aaagtgaaac ttatgtttaa gaaaaaaatg ttagctgcaa
      481 ctttgtcagt aggactgatt gcacctttag catctccgat tcaagaatct agagcaaata
      541 ctaatattga aaatattggt gatggtgctg aagtaatcaa acgtacggag gatgtaagta
      601 gtaagaaatg gggcgttact caaaatgtcc aattcgactt tgtaaaagat aaaaaatata
      661 acaaagacgc tttaattgtt aaaatgcaag gttttattaa ttccagaact tcattttcag
      721 atgtgaaggg tagtggatat gaattaacta aacgaatgat ttggccattc caatataata
      781 taggactgac gactaaagat ccaaatgtta gcttaatcaa ttaccttcct aaaaacaaaa
      841 tagaaactac tgatgttggt caaacattag gatataacat tggaggtaat ttccagtcag
      901 caccatctat aggtggcaat ggctcattta attattctaa aacaattagt tatacccaaa
      961 agagttatgt cagtgaagta gacaagcaaa actcaaaatc tgttaaatgg ggtgttaaag
     1021 caaacgaatt tgttacgcct gatggaaaaa aatctgcgca tgatagatat ttattcgtac
     1081 aaagtccaaa tggtccaaca ggttcagcaa gagaatattt tgctcctgat aatcaattgc
     1141 cacctttagt tcaaagtggc tttaatccat cgtttatcac tacactatca catgaaaaag
     1201 gttcaagtga tacgagtgaa tttgaaattt catatggtag aaacttagat attacatatg
     1261 cgactttatt ccctagaact ggtatttacg cagaaagaaa gcataatgca tttgtaaata
     1321 gaaactttgt agttagatat gaagttaatt ggaaaacaca cgaaattaaa gtgaaaggac
     1381 ataattaata tgaaaatgaa aaaattagtc aaatcatcag ttgcttcatc aattgcactg
     1441 cttttgctat cgaatacagt tgatgcagct caacatatca cacctgtaag cgagaaaaaa
     1501 gtagatgaca aaatcacttt atacaaaaca acagcaacat ctgataatga taaattgaat
     1561 atttctcaaa ttttaacgtt taatttcatt aaggataaaa gttatgacaa agatacgtta
     1621 gtacttaagg cagccggaaa cattaattca ggttataaaa agcctaatcc aaaagattac
     1681 aattactcac agttttattg gggcggtaag tataatgttt cggttagttc agaatcaaat
     1741 gatgctgtaa atgttgttga ctatgcacct aaaaatcaaa atgaagaatt ccaagttcaa
     1801 caaacattag gttattctta tggcggagat attaatatat ctaatggctt atcaggtgga
     1861 ttaaatggat caaaatcatt ttcagaaacg ataaattata aacaagaaag ttacagaact
     1921 acgattgata gaaaaacaaa tcataaatca attggctggg gtgttgaggc gcacaaaatt
     1981 atgaataatg gttggggacc atatggtaga gatagttatg acccaacata tggtaatgaa
     2041 ctgtttttag gcggtagaca aagtagttca aatgctggtc aaaatttctt gccaacacat
     2101 caaatgcctt tattggcgcg tggtaacttt aacccagaat ttataagcgt actttctcat
     2161 aaacaaaatg atacaaaaaa atctaaaatc aaagtaactt accaaagaga aatggataga
     2221 tatactaatc aatggaatcg actacactgg gttggtaata actacaaaaa tcaaaataca
     2281 gtaacgttta catctactta tgaagttgac tggcaaaacc atactgttaa attaatcggt
     2341 acggattcta aagaaactaa tcctggagta taactgttag aaatagagga agttaaaccc
     2401 gaaatttaag agttaaacaa aaaatattca caacatatta aaaaaagaag ttacattatt
     2461 tgtttaaact aacaatagta ccataaaatt aatgacgatt tacaaattaa taacaaataa
     2521 ttattattaa attcattgat ttattgtgtg gtaatgatta atatgacaat tgaagtgggt
     2581 cttaacttca acttttaaat agggggtttt atctttggaa aaagttcttg atttagacgt
     2641 gcaagttaaa ggaaataaca acactaatga ttcagcgggt gacgaaagaa taactagcca
     2701 tcttttttgt agctttggtt gtgaaaagac gggtagtttt aacagcttct gttgttaagg
     2761 atacagcaaa tagagcctga aagggctcta tttgtctttt taaatatatt aagtgctaaa
     2821 aaacggaaac agtttattaa tcgccaggta tttttataat tagccttcta tattatcagt
     2881 tgagtgtttc aataaattct gactttggtg atggaaagtt atacttttag ttgtaatata
     2941 gttttcatga cttcctgtta aataagttag gattatgtaa ttagcctcta tctttacata
     3001 taagtaatga caagctttgg ttattatatt gaccctctta gaacatgtca tttaagaaaa
     3061 taatcaagtc attgaaatta tatgtttata tcctca
//
LOCUS       DALAAR010000156         3064 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000156 DALAAR010000000
VERSION     DALAAR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.163"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(904..2103)
                     /locus_tag="O2458_002756"
     CDS             complement(904..2103)
                     /locus_tag="O2458_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ3888583.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(2096..>3064)
                     /locus_tag="O2458_002757"
     CDS             complement(2096..>3064)
                     /locus_tag="O2458_002757"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCZ3888584.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          880 a          535 c          423 g         1226 t
ORIGIN      
        1 attaagtcta attagtgtat caagaaattt ttcttgattc gttgatcgtt tcaagtttta
       61 agtctccttt taaaatgaat atttcacttc ttataactaa tatttaaaca aaatgatcac
      121 ataataatat ttaatttaca atatgccact acagtgtcaa cattttataa acactattca
      181 tttgaacatg gtctaaaacg aataggacat tgtaaatgat taaatagtct gtctctagtt
      241 gatgtatatg gtcatttgtt atgcagataa taattaatct aattaatttt tacgttttag
      301 ttacttggtt gtgattgagg ctttttgtat ctagttgtaa tattctcctt ttttagaatc
      361 ataataggta cttcttgaaa tttttagaac tttgcacatt gctgatactg aatattgata
      421 cgcattttgt tgaaagactt cattttttgt ctcaaaatta gcactgcttg ctttaaatca
      481 tcattttaca ttatcaatcg atgtttttct ttgcgtaatt ttattaactc tttttcatca
      541 cttgttaaaa tgtcttgatg attgaatgac tcagtatttt ggttttgttt tatctaattt
      601 gagaaagtta atgatgttag atcatattct cgtataattt cgtttctaag cttaccattt
      661 tcataaagtt taattatttt aatttaaatt atttactaaa agctcttcag tctcttgtca
      721 taataaaatc gcctaccctc ttagtttaac aatgtctatt ctcatagaat ttgtccaact
      781 aaatgtagac gattcactaa acttctaaaa aaataaaccc caaattataa tagtctcaat
      841 attaatatat tacaatttat tcataggatt tttatcattt caattaatag gttgttatca
      901 gggttaaata aacatttttt gtaatagtcc ttttttacgt tcttttaata actcaattct
      961 attcatttga ttattcattt tattgtctat agattttaac aatgcactta ctttatcttg
     1021 ttcagtgaga caaggtattt taagatttat attgcataag ttcgcatgag ttaatttagc
     1081 tggtgcatta cctgtgacaa atgctcgtag ttctttaaaa tttaaataat aattcataaa
     1141 aaacaaatta tgatcattac ttttaactac atgcgcatga ttatttaccc agtattgccc
     1201 attagcaata aagctactcg tctcaaactg cccccatttt gcaccatctt ctcctattag
     1261 taatcgttct tcattattga ataaataatc ttttacgtaa tcaataattc cagttgcacc
     1321 atagtaaggg tataacccct tttctcttaa tgaagaagta attggttttc ttctattatt
     1381 ttcaaagata aagatgtctt ttatgaattt gttttcccac tctggatatt cttcaccatt
     1441 ctcatctttg aatcgcaatt cttgtgtgaa gattttctgc atatagcctt ttttctgttg
     1501 ttgaagcaat tcaagttttt gttcttctaa ttcaatttgt cggtcgagtt tgctgaagaa
     1561 cttgcctatt ttttgctgtt cttcaaatat agttggggtg aagattttta aattagcaat
     1621 ttctttgaag tttagacctt ctcgactacc tccactttgt gcaaggaaaa ttttcctttt
     1681 accttttctt gataatagat actgtccaaa aaaattataa taatactctt ttttcaatct
     1741 aataatacat acatgttgat ttaaattagc atgcgtttca actatcgaat taatggctgt
     1801 tctacctatt gatgctcctg taatatttaa aagaacatca ccatagtacg ttctactatt
     1861 tttcatctca tcatctatat ctttactaat ataaactaag tcattaagat ttaatttacc
     1921 atttctaata ttttgactcc ttaaaaatgg tatgcctttg tttgtatagt tttcacttcc
     1981 acctttggga gtctttccac tacctatttt ggtagtaaga ttccctaact tcttctcttc
     2041 ccattcgcct tcaaatcctg ggaatctcaa ttctggcaca tttttctttt gtgtattact
     2101 catctttcaa caccccaagt tctttcaggt atgcattgat ttcttgctca atttctgcga
     2161 tttctttatc gatatttttc aaatcttgtt ggacttgatc taaatcaatc ggtgcttctt
     2221 cttcgaatgt atcgacatat ctcggtatat ttaggttgta atcgttatcg gcaatctctt
     2281 gtagtgtcgc gctgtagcta tatttatcaa ttgtttcctt acgcttatat gtgtctataa
     2341 tacgttcgac ttgggcatcg cttaaatgat tttgattttt tcctttttca aaatcattgg
     2401 atgcatcgat aaatagtacg ttgtcgtctt gttggcgaca ttttttaaat actaaaatac
     2461 atgttggaat acttgtccca tagaaaatat tcgctggcaa accaatcaca gcttctaagt
     2521 agttcttttc ttcaattaaa taacgacgaa tgacaccttc tgcagcacct cggaataata
     2581 caccatgtgg gagtacaacg gccatggtac cttcatcgtc taggtaatgt accatgtgtt
     2641 gaataaaggc aaagtctgct ttagacttag gcgcaagttt gccgtaacca ctgaatcgtt
     2701 cgtcattttc aaactttgaa tctgcagtcc atttcgcact atacggtggg ttcgcaataa
     2761 ccgcatcaaa tgtattgcct aaaaaggctg ggttttccaa tgtgtcatca ttacggatat
     2821 cgaagttctc ataacgcaca tcatgtaata acatattcat gcgtgctaag ttgtatgtag
     2881 tattgttacg ttcttgaccg aaataacgat acacttgtgt ttctttacca acacgtaaca
     2941 acagtgaacc tgaaccacat gttgggtcat acacgtgacg taatttatct ttaccgtctg
     3001 tgacaatctt cgccagtatc ttagatactt gttgtggtgt atagaactcg cctgcttttt
     3061 tacc
//
LOCUS       DALAAR010000157         2965 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000157 DALAAR010000000
VERSION     DALAAR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.164"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            107..880
                     /gene="lpl1"
                     /locus_tag="O2458_002758"
     CDS             107..880
                     /gene="lpl1"
                     /locus_tag="O2458_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCZ3888585.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            911..1711
                     /gene="lpl2"
                     /locus_tag="O2458_002759"
     CDS             911..1711
                     /gene="lpl2"
                     /locus_tag="O2458_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCZ3888586.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            1730..2537
                     /locus_tag="O2458_002760"
                     /pseudo
     CDS             1730..2537
                     /locus_tag="O2458_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2586..>2965
                     /gene="lpl4"
                     /locus_tag="O2458_002761"
     CDS             2586..>2965
                     /gene="lpl4"
                     /locus_tag="O2458_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ3888587.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGVWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLP"
BASE COUNT         1253 a          297 c          592 g          823 t
ORIGIN      
        1 ttgtatatgt atattttggt aacgtaaaag agaaatatac aaaataatta attatttata
       61 tgaaaagaga atataaatga agtataaaac agagagacgt gaagcgatgg gatatttaaa
      121 aaggtttgca ttgtacataa gcgttatgat tttaatattt gcgatagcag gttgtggcaa
      181 aggtaatgaa acaaaagaag attcaaagga agaacaaatc aaaaagagct ttgcgataac
      241 attagatatg tatccaatta agaatctcga ggacttatat gacaaagaag gatatcgaga
      301 tagcgaattt aaaaaaggtg acaaagggat gtggacgata tatacagatt tcgccaaaag
      361 taataaaccg ggtgaattgg atgatgaagg tatggtttta aatctggata ggaatactag
      421 aaccgctaaa ggccattatt ttgttactac attttaccgg aatggtaaac taccagatga
      481 aaagaattat aaaattgaaa tgaaaaataa taagattatt ttattagatg aagtaaaaga
      541 tgataagctc aagcagaaaa tagaaaactt taaatttttc ggtcaatatg caaatcttaa
      601 agaattgaga aaatataata atggtgatgt ttcaattaat gaaaatgttc ctagttatga
      661 tgttgaatac aaaatgagca ataaagatga aatagttaag gaattaagaa gtcgttataa
      721 tatttcgact gaaaaatcac ctatattaaa aatgcatatt gatggggacc tgaaaggcag
      781 ttctgtaggt tatagaaagt tagaaattga cttttcaaaa cgtgaaaaca gcaaattatc
      841 agtcattgaa tttttaagtt ataaaccagc gaaaaaatag tattgataag gaatattagg
      901 gtgtgaaaaa atgcgatatc taaaaaagct tgcatggttc ataagtgtta ttattttggg
      961 catttttata ataggttgtg atagttcaag cgatactggg gaaaaagcaa aagaagattc
     1021 aaaggaagaa caaatcaaaa agagctttgc gaaaacatta gatatgtatc caattaagaa
     1081 tctcgaggac ttatatgaca aagaaggata tcgagatagc gaatttaaaa aaggtgacaa
     1141 agggatgtgg atgatatata cagatttcgc caaaagtaat aaaccgggtg tattagataa
     1201 tgaaggtatg attttaaatt tggatagaaa tacacgtacg gccaagggat attattttgt
     1261 agatactata tatgacaatc atgaaaactc ttatagtaaa aattatagag ttgagatgaa
     1321 aaacaataaa attattttat tagacaaggt ggaagatcaa aaacttaaag aaagaataga
     1381 aaactttaaa tttttcggac aatatgccga tttcaagagt ttgaaaagtt acaacaatgg
     1441 cgacgtttca attaatagta atgttccaag ttatgacgcg aaatttaaaa tgagtaataa
     1501 agatgaaaat gttaagcaat taagaagtcg ttataacatt cctactgaaa aagctccaat
     1561 attaaaaatg catattgatg gggacttaaa aggcagttcc gttggatata aaaagttaga
     1621 aatagacttt tcaaaagaag aaaatagcga attatcagta gtcgattcat taaattttca
     1681 gcctgccaaa aaaaataaag atgatgaatg atagggagga tgtgaaacaa tgtggtcaat
     1741 aaaaagaatt ggaatgtaca caaatgttat aattttgagc gtttttataa taggttgtga
     1801 tagttcaagc aataatgcag aaaatcaaag agaagactca aaagaagaac aaatcaaaaa
     1861 gagctttgcg aaaacgttag atatgtaccc tattaagaat ctcgaggatt tatatgacaa
     1921 agaaggatat cgtgatggtg agtttgaaaa aggcgataag gggacgtggg tattgtattc
     1981 agctatggta tcacaaccaa aaggcgagag tttaaaatca cgaggaatga tattaaagtt
     2041 agatagaaat aagagaactg ctaaaggaag ttatattatt agagaattga aagaagataa
     2101 aaatcatgat gttcaaaaaa atgaaaagaa atatccagtg aaattggtga ataataggat
     2161 agttttgata aaagatgtta aagacaaaaa gttaaaaaaa tgaaatagag tcgtttgaat
     2221 tattttcaca atatggaaac tttaatcatt ttgatcggaa tgagattact aatatttcat
     2281 ataatcctaa tgctcccaat tactctgcag aatataaaat gaagaaaaat gacagaaaca
     2341 ttcaacagtt gaaaaagaga tttaatctaa aaactagcaa gacaccaaaa ttattgttta
     2401 agggatctgg agatataaag gggtcttctg taggatataa ggaaatagaa atcatattta
     2461 gtagaagtaa agaagaaagc atttattatg ttgacagcgt tgagttcgtt ccaagtaaca
     2521 aaataacaaa aagttgatat tagtcaacaa aagcaaatat tgagaataaa gagagttgtg
     2581 aaatgatggg ttatttaaaa agacttgtat tgtatatagt tattatggtt atgagtgttt
     2641 ttataatagg ttgtgataaa tcaagcgata ctgcagaaaa atcaaaagaa gactcgaaag
     2701 aaacacaaat caaaaagagt tttgcgaaaa cgttagatat gtattcaatt aagaatctcg
     2761 aggaactata tgacaaagaa ggctatcgag atggcgaatt tgaaaaaggt gacaaagggg
     2821 tgtggacgat atatacagat ttcgccaaaa gtaataaacc gggtgaacta agtaatgaag
     2881 gcatggtctt gtacttagat agaaacacac gcacagctaa ggggtactac tttgtcagga
     2941 cgttttatcg caaggataaa ttacc
//
LOCUS       DALAAR010000158         2890 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000158 DALAAR010000000
VERSION     DALAAR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.165"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            26..523
                     /gene="efb"
                     /locus_tag="O2458_002762"
     CDS             26..523
                     /gene="efb"
                     /locus_tag="O2458_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCZ3888588.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            677..1027
                     /gene="scb"
                     /locus_tag="O2458_002763"
     CDS             677..1027
                     /gene="scb"
                     /locus_tag="O2458_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCZ3888589.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(1680..1865)
                     /locus_tag="O2458_002764"
     CDS             complement(1680..1865)
                     /locus_tag="O2458_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888590.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(2487..2720)
                     /locus_tag="O2458_002765"
     CDS             complement(2487..2720)
                     /locus_tag="O2458_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888591.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT         1110 a          397 c          411 g          972 t
ORIGIN      
        1 gataataatt tggaggataa ttaacatgaa aaataaattg atagcaaaat ctttattaac
       61 attagcggca ataggtatta ctacaactac aattgcgtca acagcagatg cgagcgaagg
      121 atacggtcca agagaaaaga aaccagtgag tattaatcac aatatcgtag agtacaatga
      181 tggtactttt aaatatcaat ctagaccaaa atttaactca acacctaaat atattaaatt
      241 caaacatgac tataatattt tagaatttaa cgatggtaca ttcgaatatg gtgcacgtcc
      301 acaatttaat aaaccagcag cgaaaactga tgcaactatt aaaaaagaac aaaaattgat
      361 tcaagctcaa aatcttgtga gagaatttga aaaaacacat actgtcagtg cacacagaaa
      421 agcacaaaag gcagtcaact tagtttcgtt tgaatacaaa gtgaagaaaa tggtcttaca
      481 agagcgaatt gataatgtat taaaacaagg attagttaaa taaaacttca atcgttgctg
      541 ttatctggaa ataattaatt aaatgttatg ttaatttttg ttaatgaaaa aagtaatcta
      601 tttaatgaca ggttaatgta attgtcctga aattgactat atactcagta agtatcaatt
      661 ttaaggagag cttataatga aatttaaaaa atatatatta acaggaacat tagcattact
      721 tttatcatca actgggatag caactataga agggaataaa gcagatgcaa gtagtctgga
      781 caaatattta actgaaagtc agtttcatga taaacgcata gcagaagaat taagaacttt
      841 acttaacaaa tcgaatgtat atgcattagc tgcaggaagc ttaaatccat attataaacg
      901 tacgattatg atgaatgaat atagagctaa agcggcactt aagaaaaatg atttcgtatc
      961 aatggctgat gctaaagttg cattagaaaa aatatacaaa gaaattgatg aaattataaa
     1021 tagataataa ataaaacagg ttgagacaaa aaatggtctt aacctgtttt caatttgcat
     1081 atgtgataaa ttctatatca aaatgcttat gtataatgaa tgacatttaa aagtagggga
     1141 gacaaatata aatacaatag ttcctaggat tactctcaaa ataactatat caattattta
     1201 ctttgctctc ctatttttta aaatatctac atgtttaaac aatcaaaagt gtacaatatt
     1261 aaattatcat ttccagttct agtgctatat tggtagtagt tgactaaatg aaaataagct
     1321 tataacaagt tttttcaata ctcgtggggc cacaacagag agaaatagga tcaccaattc
     1381 caacaaacaa tgcaagttgg cgggggcccc aatatagaag ctggcgaaaa gtcagcttac
     1441 aaaaatgtgc aagttggcgg ggccccaaca tagagaaatt ggaaccccaa tttctacaga
     1501 caatgcaagt tggggtagga catcgataaa gaaatacttt ttctttagca attagtattt
     1561 cttatgcatg agctttactc atgtattcat tttttaagta cacattagct acagctaatg
     1621 ataaagaacc actacataat aaatcattag tggttcttta tcatttctat ctcactcttt
     1681 tactggaaga aaaagtttac gtttgtagaa catgccacaa taccaaaaat aattaagaaa
     1741 aataagacga taagcatgat gacacttttc aaacaacctc tatcagtttc tttcgatttt
     1801 ctttgttgaa cctttttata atcttcaagt agttttgcgg cttttttatt tatatgttta
     1861 ttcatgatgt tgactcctta taatatatgt ttaattcatt aaaatagttg aaaacatgac
     1921 ttgaaataaa gatataaagc taaagtaagg atgttcaatt gtttcaaatt ttatgaaagt
     1981 ataacgttaa atatgttaat ttgatgatga tatttgcttt ttattttcca aatggaattt
     2041 acttaaactg atgcattaaa atattaatga agcactagaa tacataaatg aatagtaatg
     2101 gtgcacagta tagaataatt aaggctatat taagtataaa tatcgttaac tgtaagctat
     2161 ctttagtttt aatataaact attaggataa tcgacgtaag aagaatcata tatattaatg
     2221 atgaagaagt ccatacaaaa tccgcatcat ttgttgttaa taatgggact ataattaatc
     2281 cgaaattaat catgcatgct atatatacta taatgttata cacaatgtta atttttgttc
     2341 accaccttat acttctattt taaaaacttc tttataatga tatattgttt aatgttgaaa
     2401 taattagatt atctaatttt catttgcttt acatgtaaaa ggctatatat agtatgctct
     2461 ttatgattct aaatgctttt taatatttaa tgctcatcaa catttggatt ttgaatattc
     2521 aattcaaaaa ctttattagc tacgtcaatt gtaaaatcag aaccatagtt gacatgagct
     2581 acttttaatt ttccatctaa ataatagatt gcgattgcaa catcgtaaaa ttcgtcaatg
     2641 acaaataaac tcttttcgtt tgttacaacc tcatgctctc ctgagtatac aacgttaatt
     2701 tcccaatcat taaaaaccat ttgttaacct ccttgaacat ttaaattgat tcaacttaag
     2761 tttaacttat tcatacaact tcgtacaata tctagatgaa cattaattgt atttctagaa
     2821 atctttttca attatatgta ctaattatac ttttaaattt cttatttcag tatagtttta
     2881 aaacgatttt
//
LOCUS       DALAAR010000159         2879 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000159 DALAAR010000000
VERSION     DALAAR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.166"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..556)
                     /locus_tag="O2458_002766"
     CDS             complement(<1..556)
                     /locus_tag="O2458_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ3888592.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNE"
     gene            623..1093
                     /locus_tag="O2458_002767"
     CDS             623..1093
                     /locus_tag="O2458_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCZ3888593.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            1240..2109
                     /locus_tag="O2458_002768"
     CDS             1240..2109
                     /locus_tag="O2458_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ3888594.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            2130..2696
                     /locus_tag="O2458_002769"
     CDS             2130..2696
                     /locus_tag="O2458_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ3888595.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         1082 a          396 c          498 g          903 t
ORIGIN      
        1 gttcattttt aagaaaatca atagaagttg tatcgattaa aactacattt gaaccattac
       61 tttgtaagga ctcataatac tcacgataat ctttttttaa ctttaacaga tattcatctt
      121 ctatttgatg ctcaaaacta cggttacgtt tagcaattct agattttaac acatcaaggt
      181 ctgcatctaa aaagataatc atattcggca taatcatatc ttcagttaaa atatcataaa
      241 ttttactgaa tttctgaaat tcaacagaac tcaaagtatt tttagcaaat atcttatttt
      301 tatgtatatg ataatcacta actacacctt gatttagttg tgttacatct tgaaattgct
      361 tatatctatt gcataaaaag aacatttcag tttgaaaact ccatttagag atatcttcat
      421 aaaagtctga taaaaatgga ttttctgtga tgatttcttt ttcttcataa aaatctaaag
      481 tttgacttaa tttgtgtgca agtgaagatt tacctacgcc aataggacct tcaattgcta
      541 taaaaggttt gttcatattc acatctccaa atgttaaaat agacattgag tattgtacca
      601 taagtgaggt atctggacat aaatgacaaa tgatatatat tttatgacat tagcgattga
      661 agaagctaaa aaagcagctc aactaggcga agtacctata ggtgctatca tcactaaaga
      721 tgatgaagtt atcgctagag cacataattt aagagaaaca ctacaacaac caacggcgca
      781 tgctgaacat attgcaattg aacgtgcagc caaagtgtta ggtagttggc gtttagaagg
      841 ttgcacatta tatgtaacct tagaaccatg tgtcatgtgc gcaggaacaa ttgtaatgag
      901 tcgcattcca agagtcgtct atggcgcaga tgatcctaaa ggtggttgta gtggcagttt
      961 aatgaattta ttgcaacaat ctaattttaa tcatcgtgca attgttgata aaggtgtact
     1021 taaagaagca tgtagcacat tattaacaac attttttaaa aacttaagag ccaataagaa
     1081 atccaccaat tagacgatag tacatactaa ttaaaatttg ttagaattac attatatgat
     1141 aaattaatga caatataaat gttaatagaa aagtatgtgc gctgagtata tattcttatt
     1201 taagaacaag attttaaatt tacgaaacga ggtaacataa tgataaaact aatagccact
     1261 gatatggatg gcacgctact taatgcagca catgaaattt ctcaacctaa tattgatgcg
     1321 attaaatacg ctcaagaaca agggataacg gttgttatcg cgacaggtcg agcattttat
     1381 gaagcacaag caccagttgc tgacacagat ttaacagtac catatatttg tttgaatggt
     1441 gctgaagtac gtgatgaaac tttcaatgta atgagcactt cacaccttaa taaatcgtta
     1501 gtacacaaaa ttacaaatgt tttaaaagat gcaggtattt attatcaagt atacacgagt
     1561 cgtgcgattt atactgaaga tccacaaaga gatttagaca tttacataga tattgctgag
     1621 cgtgcaggtc aacatgcaaa cgttgagcgt attaaaaatg gtattcaaag acgcatagat
     1681 aatggtacgt tgaaagttgt tgataattat gatgctattg aaaacatacc tggtgaatta
     1741 attatgaaaa tattagcatt tgatggaaat ttagaaaaaa ttgacaaagc tagtaaaatt
     1801 ttagctgaat ctccgaattt agctatatca tcatcttcga gaggaaatat agaaataacg
     1861 cattcagatg cacaaaaagg tattgcgcta gaaacaattg ccgaaagatt agggattgaa
     1921 atgaaagaag tcatgtcaat aggtgacaat ttaaatgact tatcaatgtt agagaaagtt
     1981 ggctatccag ttgcgatgga aaatggtgca gaagaagtta aaaaaatagc gaaatatgtc
     2041 acagatacga atgaaaatag tggtgttgga aaagctatta tgaaattatt acgtgaacaa
     2101 caagtttaat aaaaaaagag gggtcaaata tgaaaggatt aattattatt ggcagtgcac
     2161 aagtgaattc acatacaagt gcactagcaa gatacttaac tgagcatttt aaaacacatg
     2221 atattgaagc ggaaatattc gatttagcag aaaaaccgtt aaatcaatta gatttttcag
     2281 gaacaacacc gtctattgat gaaatcaaac aaaatatgaa agatttaaaa gagaaagcaa
     2341 tggcggcgga ctttttaata ttaggaacgc caaactatca tggttcatat tctggaatat
     2401 tgaaaaatgc attagatcat ctaaatatgg attattttaa aatgaaacct gtaggcttaa
     2461 taggaaatag tggtggtatt gttagttcag agccattgtc acatttaaga gtaatcgtca
     2521 gaagtttact aggcattgct gtaccaactc aaatagcaac acatgattct gattttgcta
     2581 aaaatgaaga tggttcatat tacttaaatg atagtgaatt ccaattacga gcaagattat
     2641 ttgtcgatca aattgtatct tttgtgaata atagtccata tgaacattta aaataatatt
     2701 aaaaaatatg taaatataca ttaaaaatag gtatgtagct taaggtattt cattgagaat
     2761 gcttttaagt cacatgccta ttttttgttc taatgcacca atagtaagcc tttgatatta
     2821 ctgttgtgag caatgttatt aattaaaata agatgtaata gcgaattgaa aacaagctt
//
LOCUS       DALAAR010000160         2842 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000160 DALAAR010000000
VERSION     DALAAR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.167"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            34..1110
                     /locus_tag="O2458_002770"
     CDS             34..1110
                     /locus_tag="O2458_002770"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCZ3888596.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1360..2043
                     /locus_tag="O2458_002771"
     CDS             1360..2043
                     /locus_tag="O2458_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ3888597.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2159..2821)
                     /locus_tag="O2458_002772"
     CDS             complement(2159..2821)
                     /locus_tag="O2458_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ3888598.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
BASE COUNT          997 a          399 c          534 g          912 t
ORIGIN      
        1 attcttatac aatattatta ggaggcaatc accatgtcac aagcagttaa agttgaacga
       61 cgagaaacat taaaacaaaa accaaataca tctcaactag gttttggtaa atattttact
      121 gattatatgt tgagttatga ttatgatgca gataaaggat ggcatgattt gaagatagta
      181 ccttatggtc ctattgaaat ttcacctgct gcacaaggtg ttcattatgg tcaatcggta
      241 ttcgaaggat taaaagcata taaaagagat ggggaagttg cacttttccg tcctgaagaa
      301 aattttaagc gtcttaataa ctcgttagca cgattagaaa tgcctcaagt agacgaagca
      361 gaattgttag aggggctaaa acaattagtt gatattgaaa gagattggat tcctgaaggg
      421 gaaggtcaat cattatatat tcgtccattt gtttttgcaa cagaaggggc acttggcgtt
      481 ggtgcatcac atcagtataa attattaatt attttatctc cttcaggtgc atattatggt
      541 ggtgaaactt taaaaccaac taaaatctat gtagaagatg aatatgtgcg tgctgttcgt
      601 ggcggtgtag gctttgcaaa agttgcaggt aactatgcgg caagtttatt agcacaaact
      661 aatgcaaata aattaggtta tgaccaagta ttatggcttg atggtgttga acagaaatat
      721 atcgaagaag ttggtagcat gaacattttc ttcgttgaaa atggcaaagt aattacacca
      781 gagttgaatg gcagtatttt acctggtatt acacgtaaat ctattatcga attagctaaa
      841 aacttaggat atgaagtcga agagcgccgc gtttcaatcg atgaattatt cgaatcatat
      901 gataaaggtg agttaacaga agtatttggt agtggtactg cagcagttat ttcacctgtg
      961 ggtacattga gatacgaaga tcgtgaaatc gttattaata ataatgagac tggtgaaatt
     1021 actcaaaaat tatacgacgt ctatactggt attcaaaatg gtactttaga agataaaaat
     1081 ggttggagag tcgttgtacc aaaatattaa taaaaattga atataatcat gaaaacaata
     1141 tgcaaaagtt aatcatgact aaaatccctt tcaatgaagc gaagcatggt aataaattaa
     1201 gtttacaatg tttattgtta agtattgaag gggatttcac ttattattat atttaattca
     1261 atatttaaat agaacaatac tacgagttca ttttcagggg ataacgaata tcatagaatc
     1321 agcataatac gaaataaata aataggagta ttgatatcaa tggaatggat attatttgat
     1381 aaagatggta cgttaattga atttgataga agttgggaaa aaataggggt acgatttgta
     1441 caatcattgc ttgagacttt cccagtacat aataaagaag ctgctttaag acaactcggt
     1501 gtcattaaag aatctattga tccaaaatca gtgatgggtt caggatcttt acaacaaatt
     1561 atccaggcat ttaatgatgt gacgggacaa gatacaaccg actggtccaa gtcaacaagt
     1621 caaaagctgg tagatgaacg tattcctgaa attaattggg tagaaggtgt taaagaagca
     1681 cttatcgatt tgaaagcaaa aggctatcaa cttggtattg ttacgagtga tactaaaaaa
     1741 ggtgtagaac aatttttagc acataccaat gctacctcgt tgttcgattt gatcatttct
     1801 accgaagcgg atgcctatga gaagccaaat cctaaagtat tatcgccttt atttgagcaa
     1861 tataatgtag atcctcagaa agtagctata gtaggagaca ctgctaatga tatgaagaca
     1921 gcaagtaatg caaatttagg tatggcaata ggtgtattaa caggtattgc aacaaaagaa
     1981 gaattacatg aagctgatat tattttaaat agtgcggcag atattttaga agctttaaat
     2041 taaaaagaaa gacatagtgt atatgtgaat taatgtattc gatagaattg ataaaataat
     2101 cgataatatc tatggaatat acattgaata taaacatata cgctatgtct ttagtctttt
     2161 atcgtgtatc tacttgtcga tatgtttgaa taattcgagc aattttgttt atcataggat
     2221 ttaaagattc ggggtcctta tggatatcat attcattaat attgatacgt acaactggac
     2281 atgcattaaa gctattaatc caatcgtcat agcgtttaaa tagctttttc cagtattcag
     2341 ggtctgtatt aatttccatt tcgcgaccac gttcaataat acgatcaatg acctcatcat
     2401 agttacattc taaataaatc attacatcag gtttaggaaa ataaggtgtc atgaccatgg
     2461 cattaaataa gtctgaatat gttttgaaat cttctttact cattgtgcct tcttcttcat
     2521 gcatttttgc aaaaatatca acatcttcat aaattgatcg atcttggaca aagccaccac
     2581 catattcaaa catacgcttt tgttctttaa aacgttcagc taagaagtaa atttgcaaat
     2641 ggaaactcca tcgttcaaaa tcgctgtaaa atttatctaa atatggatta tgttcgacat
     2701 tttcaaaaga cgttttaaag tttaatttat ctgcaagtgc ttgcgttagt gttgattttc
     2761 caacaccaac tgtacctgca atggttataa tggcattttg tggaataccg taattattca
     2821 ttggtaatat ctcctatcat ag
//
LOCUS       DALAAR010000161         2839 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000161 DALAAR010000000
VERSION     DALAAR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.168"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..1369
                     /locus_tag="O2458_002773"
     CDS             <1..1369
                     /locus_tag="O2458_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCZ3888599.1"
                     /translation="LRKIIFENFSKKCTYWVTLIITQILLTTSVLWILNYTGNLDLFI
                     LRIVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSILLVLI
                     ANVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKA
                     SEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVE
                     VIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDCRIFSFDARDSRHL
                     SFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYK
                     DSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGYSTL
                     RANYQYLLINNIVVIFIGILTNVILHSHYIMMLFATILVVQILLQICSLYYHGRRFNE
                     VIKEF"
     gene            1372..1692
                     /locus_tag="O2458_002774"
     CDS             1372..1692
                     /locus_tag="O2458_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ3888600.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1689..2330
                     /locus_tag="O2458_002775"
     CDS             1689..2330
                     /locus_tag="O2458_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888601.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(2418..2708)
                     /locus_tag="O2458_002776"
     CDS             complement(2418..2708)
                     /locus_tag="O2458_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888602.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         1122 a          344 c          446 g          927 t
ORIGIN      
        1 attaagaaaa ataattttcg aaaatttttc aaagaaatgc acgtattggg taacgttaat
       61 cataactcaa attcttttaa ctacaagtgt actttggata ttaaattaca cgggtaattt
      121 agatttattc atattaagaa tagttctgct ttcatgtctt ttcattttaa cgattagtgt
      181 tattaattta tggactttct taatgttact aaatttaaat attgcaaata tgattaaagg
      241 taagcagcac tttaaaacaa ttcgttttat taatacagtt tgtaaaagta ttctcttagt
      301 actaatagct aatgtaatga tagaaaatac tagtgttatt aaagatttga ataaaataaa
      361 agaaactgag aaatattgga atgtattaga tgattattac acgattgaat ttgcacctta
      421 tcacgaaaca aaacaaagtt tgattgataa tatggtgcga tcagaacaat tagtaaaggc
      481 tagtgaagca gaaaataatg cgattttatt caaaccaaag ggtgactccg ttgacaatga
      541 caacttttcg cctgatgagg ggaatgtaat attagtaaat aatcagttct ggtcgattta
      601 ttacaagcag tttcaacctg atattccgat aaaaaatcaa aaaaataatg tcgaagtaat
      661 tattccacaa aagtttcatg caatgcgtaa tgaaatcaat caagcatatc attcatggtt
      721 tgaatttgta caaaataaaa ataataaaga gaataagtta tctatacagt ttatcaacaa
      781 aaatgattgt cgaatttttt catttgatgc acgagatagt cgccatttgt catttataga
      841 ggcgccaatc attgtgaatg ttcaggcatc agatttatcg aatgattttt attatgccat
      901 gatcagtcaa ggcgggtatt tattcaaaaa ttatgacgcg ctagtaaaaa atattgaaaa
      961 gtatcatctt gatggggaaa tcagtggaat aaccaattat aaagatagcg tgatggaaat
     1021 gtatcatgaa aacaatttga aattaacagt actcaacttt tcacaaatca ttatcgcaat
     1081 cattttaata attattattt tatttgatgt gaaatattat tttgaacagc atcgaaaatt
     1141 actcgtaatc aaaaagctat atggttattc aacattaaga gccaattacc aatacttatt
     1201 aataaataat atagttgtta tttttattgg aatattgacg aatgtaattt tacattctca
     1261 ctatataatg atgttatttg caacgattct tgtcgttcaa atattattgc agatttgcag
     1321 cctatactat catggccgac gttttaatga agttatcaag gagttttaac tatgaaattt
     1381 atcattgcaa tattattagg attaatatta tccattacta ttgcttttac aatcatacat
     1441 catcctatac ttgatcgttt taatcctttc ttaaaaacgg agtatagtta tgccaaagtg
     1501 ccaaaaggta cgcaacaata tgttaatatt acggcttata gtgaaagagg ggaaaagctt
     1561 gattataaat taacatttaa tggattttca cctagtagaa cgtatgtcga aataaagcat
     1621 aaagggcaat atgtcatatc gatcacatat gttgaaaaag aggatacacc aaaagaggta
     1681 agacaagaat gatagaatta aaagatttaa ccatacaaaa aggtaataca catattttga
     1741 agaaacttaa tttgaaattt caatgtggaa aatcatatgc acttattggt aaaagtggat
     1801 gcggtaaatc tacattatta aatactattg ctggacttga aaaaacagga aaacaatatg
     1861 tctattttaa tggtcaatta gaacaattta aatctaattt ttacagagat aaattaggat
     1921 atttatttca aaattatgga ttaatcgata atttgacagt aaatgaaaat ttagatattg
     1981 gattagcata taaaaaaata agtaagaaag aaaaagaaca aataaagata cgttatatag
     2041 aacagtttgg tctgtcaaac agtttaaaaa gaaaagttca tacgctaagt ggaggtgaac
     2101 aacaacgtgt cgctttaatt agaatgatgt taaaagatcc gattgttatg ttagctgatg
     2161 aaccaacggg tgcgttagat cctaaaacag gacagatgat tattcaatca ttatttggtt
     2221 tggtcgatga aaataaagtg ctgattttag caacacatga tatggctatt gcaaatcaat
     2281 gtgacgaaat aatagattta gaacagtata gaaaagtagc atctatgtga tatccaaaag
     2341 ataaaattaa aagccaatca atgggaaatg ttgacttcaa cactccgttt gattggcttt
     2401 tatactgtgt tactaagtta tgaaagtaac aacggcaaac tgaaaaaaag caatataata
     2461 atatttaata cgattagtaa taaatgtaat tgatttttcg ttttaatata gcaaaaaaca
     2521 atgactaaag ataacaacaa tatcatgatt gcacctggga agaaccataa tgctgaagct
     2581 gtgtaccaaa tcattggtaa cgccataata ataaaagata taagacaaga aaggatacca
     2641 ataatattat aagttctagt caaaattaca cctccaattt tcactttaaa atgacatgcg
     2701 taaaacaatc attttaaata cattatacaa taaaattaaa ttgtgcggta gaagaatttg
     2761 aaattaaatt gaattgtttt tggtagttgg tatatttata acactctgca aaagaatgta
     2821 gcaaaattag ataaattag
//
LOCUS       DALAAR010000162         2834 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000162 DALAAR010000000
VERSION     DALAAR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.169"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            112..651
                     /locus_tag="O2458_002777"
     CDS             112..651
                     /locus_tag="O2458_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888603.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            664..1451
                     /locus_tag="O2458_002778"
                     /pseudo
     CDS             664..1451
                     /locus_tag="O2458_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            1912..2120
                     /locus_tag="O2458_002779"
                     /pseudo
     CDS             1912..2120
                     /locus_tag="O2458_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2694..2782)
                     /locus_tag="O2458_002780"
     tRNA            complement(2694..2782)
                     /locus_tag="O2458_002780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2745..2747),aa:Ser,seq:gga)
BASE COUNT         1132 a          388 c          399 g          915 t
ORIGIN      
        1 atacttaatt ttaaaaatcc cctatgaaaa tgttgatgag ctacatcttt aaatcttatt
       61 atacattgta taaaattata ttgcgaggta gtaaattgat attatacact tttggttgcg
      121 tatgcatctc acctatcttt tttgcttttt tcaaataaga aagtattaaa aattaatgcc
      181 tatacaggaa cagacatggc taaacttgat ttaaatagtc ttgacgacga gcacgtaaaa
      241 ttattaataa atgaattaaa atatccagaa actcatatcg atgtaaatga attaaaaaca
      301 atagttgcta gtcgaataaa tgaaaggcaa gaaataataa gttttaagtt aggaataaag
      361 tacttattaa caataaaaag agggaacata gaaaaagata ggttttcaat ttcaatcatt
      421 ttcaaagata cctatcacac cctagttaga atagatatta acggtggtac tcacgataat
      481 ccagatggaa caatcgctcc gaaaagtcat attcacatat ataatgataa gtatgataaa
      541 aaggataggt ttgcttatga aattaacttg aaagatttcc ccgacatcta taacttgtat
      601 aatgtgtata tgtcgttttt agagtataat aatataaaag accttgaata aaggagggat
      661 aatatgacta catttgatgc taaaaaatta aaaaaagaat atcttgattg gtataatcag
      721 accttagagt tttctaattt atcaaacaat gtagtaagaa tagatactcc ttttaaagat
      781 aattctttag ataatttaat aatttacgct ttatacgatc agtccagaga catgattaca
      841 ctgacagatg acggctatac tatatttgat ttagaaaata atggtatttc tttaaataaa
      901 tcaaaaaaac gtaaaaagat ttttgaagag cacctttcag cttacggtat taaatataac
      961 gataaaactc acgaaatttt tgttcaaact aactttaaaa attttaataa atcgaaacat
     1021 aatttattac agtgccttat atttgttaat gatatgtact tactttctaa tcctaagtca
     1081 cagaacatat ttacagaaga tgttgcaaac aaattggatg aacataacat ttattacgga
     1141 agagatttac ctattatagg aagcagtggt gttgttcata atttcgactt ttttattagc
     1201 gctaagaaaa atcaaaaaga aaaatttatc aatgctattt ctaaccctaa taattctatg
     1261 attattaagt cgaaaataac ggatgctatg caagcaaaaa aataaaaaga cacaggcaaa
     1321 atgagtttat ttttatttta aatgactcaa aaaaagaaat aaatgaacat aataaaaatc
     1381 ttcttcatga aaactatatt agtacaatag attatagcga attagatgaa aagataggtt
     1441 tattgattta atatatatag acgtgataat atcaatgttt atattaaatg aaacgaattt
     1501 gaaaatttcg aaacagctta agatagcaaa ttgaatagcc ttattgataa tgcagaatcg
     1561 tctacactta gttgaacaaa ttctatgaga atagatattg ttaaactatt tgggtaggcg
     1621 attttatttt gacaagagtc agaagattat ttagttcata gtttaagtta caaatggcta
     1681 aactttatga aaatggtatg actagaatcg aaattatact cgaatacgat ttaacactct
     1741 caatcttctc gaactgaata aatactgggt cattcaatca tcaagacaac ttaataagtg
     1801 atgataaatg ttcataccat tttgcaaata taaccagata ctctcaacaa aatgtagagt
     1861 tcaaaattaa aaatgtccct aattggcaca ttgctgtatg ataataaatt caacttagat
     1921 ttcaacaata taactaatga tgcttatatt taccgcgata tcaaaccaaa cagcccatcc
     1981 acaaacaaat tagaatatat catgttaata atagtaaatg atatatcttc caaagaccgc
     2041 aaactgtagc agaacatgag aaccttgcga ttttgaaatg gtctaataaa tcaaatatca
     2101 ttgaaacttt aatcacataa attaacagta gtcacctgtt cgcagttcta taaattacaa
     2161 atgcaataaa ggctaaagta aactaattac tttagccttt attaataatt tagtcgaatt
     2221 tattaaaaat cgactaactc ctggttatgc tacgctttac tttataatcc gcacgtagat
     2281 gccgattgat tatattataa tattattatt ttattttttc aagcgtatat ataatatttg
     2341 attttcttta agtaacagct aaacattaca tttcaaaacg attaggtcat gcagatgtac
     2401 atacaacgct taaaacatat tcacatttga ttaaagaatt tgaagataat gaaactaaat
     2461 ttatagaaaa aaatctgaat gatttgtttt gtgactaaac ttcaaaatct tgtgactaaa
     2521 attaggatta ttcatcaaaa attagtcaca ttgtggctaa aaaattttaa atggatataa
     2581 ataaataaga agtgtaaaaa tgaggataat agctcaaatg cagatatatc aagggattga
     2641 gtccaaaata aaaatagcgc ttaaaatggg taaaaccaat ttaagcgcct ttacggaagg
     2701 taagggattc gaacccttga gacgcttgtg acgcctacac actttccagg cgtgctcctt
     2761 cggccagctc ggacaacctt cctcaacaat aaatatgtaa taaaaaaaca gaagcgatat
     2821 ttcacttctg cttg
//
LOCUS       DALAAR010000163         2761 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000163 DALAAR010000000
VERSION     DALAAR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.170"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(905..1099)
                     /locus_tag="O2458_002781"
     CDS             complement(905..1099)
                     /locus_tag="O2458_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888604.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1209..1901
                     /locus_tag="O2458_002782"
     CDS             1209..1901
                     /locus_tag="O2458_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888605.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(2069..2218)
                     /locus_tag="O2458_002783"
     CDS             complement(2069..2218)
                     /locus_tag="O2458_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888606.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
BASE COUNT         1091 a          428 c          374 g          868 t
ORIGIN      
        1 tcggcataaa tgcgtgtcta tgcgtagaca tggagacact tcagaattat ggagtgatta
       61 catgagttta actatttttc tttattcagt tttaggaagt ttattagcaa attatatcta
      121 cgaaaaatct tcaaaataaa taactttttc ggttctactt tgtacacttt atctacctgg
      181 tagacctaat tgttattgct ttacaggtct aaaattcgtc gttataatct agttaaatcc
      241 cgtaagggcg cacttatacg tcggataaat cgacatgtgc aaaaacgtaa tcgaccttcg
      301 tcgatttttt tatttcaaaa ttaaagaaac ggcttgagat attccgagca gttataaaaa
      361 ttaatttgcg ttgaatattg ctactacatt cttgaaaaag tgatgtagat ttatagccca
      421 taactttttt gaattacatc gacgatacgg cgtcgaaaga aaaataaata gaccgtatga
      481 aagaaaaagc tacctcactt tatctggggt agcttttatt ttttatgccc ttattttgaa
      541 ttttaagagg gcattttaaa gatttagggg tgattcatat agttttatat ctaaaactta
      601 tatatgcttt taaaacgcaa atatgagcca aataaatata tgctattttc aaaaacaaaa
      661 atttgagcaa aaacaataac tatttttaga cactgcccat ttacatgcaa attaaaaatt
      721 agcataaaat tctttaaaac ttccccacgt ttttctaaat tcctgtaggg cgtttttatc
      781 aaaaatcaag acaagaagaa actcgttcac actcgtttca aaaaaacaag aaatctaatc
      841 tatacaccta ccgttttcca accccctcaa agcatacaaa ttaaaaacaa aattattcat
      901 aatcttactt tcttttaaca aaaacataaa aaccataaat agacaaatta actatcaaag
      961 gcaataaaca gtataaaaaa accacgaata aatcttcatc aacatctaaa ataaaaaaga
     1021 ataaaacaat taacaaaata ctaattaaca aacctaaaag tggcaaactt tttatatatt
     1081 tttctaaatt ttcattcata aaatagactc ctttcaaaaa tatgtttata aatatattaa
     1141 aataaatatt gattttataa agcgcttact tatacaatta aaatatctta ttatcttgga
     1201 ggtatcaaat gaaaattata aaattatttt caataatttc cttgttccta ctggtattta
     1261 gcgcgaattt ttctaatgct tacgcaaatg aagaacaaaa aagttcttta ctagaaaatc
     1321 aaaaagaaaa attgaaaagc aaaaacatcg aagtatcaca aatgaccaag aaagaaataa
     1381 gttttctaga acaattgcaa aataaaaatg aagatatgca aaaattcgaa aagcaaatga
     1441 aaaaagaagg atttaaaaaa ataaagctag aagacaaaaa ttctaaatta aaatatctag
     1501 ctacagaagc aaatgataaa aatgcatatg gctacatagc taataacgct tataaaaatg
     1561 aaaaaacaaa agaaattgta gtttctgaaa caatatatga taactacaac aaaaaaatca
     1621 caaaattcat tgccgaaaaa agaagtatga ctgaaaacaa agagggaaaa atacttatta
     1681 acaaaaatta cgttgaatat aaggctgaaa aagttgataa aaacagaccg gtaacatatg
     1741 caggattcaa atggaacggc aaagcattcg cttgtagtat ggctggactt tatgcatgtg
     1801 ctcactactg tggagtatgg gcaatcgtaa atccaatagc cggcggtgta tgtgaagcag
     1861 tttgcggaac tgcatttgca gctggatgct ctgtaggttg attttattcc aattgtttta
     1921 ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata
     1981 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata
     2041 aattttttcg gcataaatac gatgttcctt atttatcctc ataaaaaaca atagctgaaa
     2101 tatataacca attactgtgt gtttgaattt ctaatacatg aatatcattc tttaaaaacc
     2161 tattaacttg tttatctaat tgacgatgag taaaagtagc gaaagtttta atcttcatga
     2221 tatctctcct tttataaaaa tagcaatcct tattacaata ttaaacactt ttattttgga
     2281 ttttaagagg cattttgaag acttagaggc aaatcatata ggttttgtac ttaaaaatct
     2341 atacagtctc ttaaaacgca aatatgagcc aaataaatat atgctaattt cacaaacaaa
     2401 aatttcagca aaatcagtga ctaatttttt agatactgcc catttacatg caaatttaaa
     2461 aattagcata aaatacttta aaacttcccc atgtttttct aaattcctgc agggcttttc
     2521 ttatcaaaaa cttagaaatt ttaatttaac tctaaaatct ccgtaagagc ataagggaaa
     2581 tttgctatca ttcaaaaatg atagatctgg caaggccaga cattccaagg gttttaagtt
     2641 attttaaagt gatatcatac aaagctgtag tgtcacactt ttgtgacact acatacagca
     2701 tttgtcacaa aaataagaca gcactttttt acagaaaaac aataaaaaaa gacatcagtt
     2761 a
//
LOCUS       DALAAR010000164         2749 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000164 DALAAR010000000
VERSION     DALAAR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.171"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(24..169)
                     /locus_tag="O2458_002784"
                     /pseudo
     CDS             complement(24..169)
                     /locus_tag="O2458_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(402..1004)
                     /locus_tag="O2458_002785"
     CDS             complement(402..1004)
                     /locus_tag="O2458_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ3888607.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(1019..1195)
                     /locus_tag="O2458_002786"
     CDS             complement(1019..1195)
                     /locus_tag="O2458_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCZ3888608.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1394..2380
                     /locus_tag="O2458_002787"
     CDS             1394..2380
                     /locus_tag="O2458_002787"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ3888609.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(2461..2679)
                     /locus_tag="O2458_002788"
     CDS             complement(2461..2679)
                     /locus_tag="O2458_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCZ3888610.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1038 a          404 c          398 g          909 t
ORIGIN      
        1 atgtcaaagt tttgaaatga tggttatttt ttattgctta aatttattat tgctactact
       61 ataccaatga aagtgacgat aaacataccg aaacttaaca ttaaatgtaa tgcgtttgca
      121 atagatatca caataggctt ttcctttcaa aagtgttaag taatgccata ggcaccaccc
      181 ccctttactc agatagccac catcaattca acttgctcaa aaatattata ccatttttat
      241 aaaatattta gtaataaaaa ctgactaaaa cctaatctca ataataaccg cttgctgtta
      301 aaaaatatta ccactataat gaagcatgcc tcgaatcttc gtattgctat tcttaagcaa
      361 aaaagctttt cttagaacac atacgtctaa gaaaagctta tttaagcatt aaaaattaat
      421 aacaatataa tagctacaat ttgtgcaacg gatgttgttg ctatacgtcg agtatactca
      481 taacttaatg ataatatcac ttgtgcaaca aatataaatg ttactatcca ccaattatct
      541 aaataaataa ataatgaact agaacaaatt gcagttatta atatagctac ccatttcggt
      601 attgaaaaat gtgttaattc tttttgtaaa acagttctca tatataactc gtgacatgga
      661 atgacaaaaa gtaaagttac taacatttgc cacttccaat tgacacctgt tcttgctaat
      721 tctttaatta atgaactata acttaaatta gaagttataa gtgagaataa aagttgaagc
      781 ataattaaaa taattgccgt aataatacca acaccaattg atgtaagcaa ccgcttagat
      841 tcaatatctc tttgataaaa tacataactg ataccagcaa ttagcattat acctgtatat
      901 aaataccagt attcttggtg ttcgccccac ataataaata atatgagatg aaatataatg
      961 aaacttataa taaaccaagt taaagccttc gagattttat tcattataac ttaccttctt
     1021 attcaacaat cttatatcgt ttatgattga taacagtttt ttccggtaaa tcaaggtcat
     1081 taaaattttc cataattgtt aagtcaacaa taacagagtt atcgttgatt ttttcaacac
     1141 gacctcttaa tccatccata aactcaacaa tgttacccac ttctgcaaca gtcattttaa
     1201 gtcctcctta aatcataact aaattatatt gtactaaaat tcacttaaca aataaactat
     1261 taacaatcaa aaataataaa atgtaacttt aagccgatat ttagaatgat ttgtgagtat
     1321 ataattggca ttttgagcaa agttaaggca taaataagta gagaatatat atagatataa
     1381 gggagagatt gtaatgaaat tcattagtaa taataatatt acagatccaa ctttaaactt
     1441 agcaatggaa gaatatgttt taaaaaattt accagcagaa gaaagttact ttttatttta
     1501 cataaataga ccatctatca ttgttggaaa gaatcaaaat acgatagagg aagtaaatca
     1561 gacatatatc gatgctcaca acatcgatgt agtaagaaga atttctggtg gtggcgctgt
     1621 ttatcatgat actggcaatt taaactttag ttttataaca gatgatgatg gtaacagttt
     1681 ccacaatttc caaaaattca ctgaaccaat tgttcaagca ttacaatctt taggcgtaaa
     1741 tgctgaatta actggtcgta acgatattca agttggacaa gcgaaaatct cagggaatgc
     1801 tatggttaaa gttaaaaata gaatgtttag tcatggtacg ttgatgttaa atagtgattt
     1861 agacgaagtt cagaatgcac taaaagttaa tccagctaag attaaatcaa aaggtattaa
     1921 atctgttcgt aaacgggtag caaacattca agaattttta aatgaccctt tagaaattga
     1981 agaatttaaa aaaatcatct tgaaaactat ctttggagaa actgaagtag aagaatataa
     2041 attaacggat gaagattggg aaaatattga aaagttaagt aacgacaaat atagaacttg
     2101 ggaatggaat tatggtagaa accctaaata taactttgaa cgcgaggaaa aatttgaaaa
     2161 aggatttgta caaattaagt ttgatgttaa acgaggtaaa atcgaacatg caaaaatatt
     2221 cggtgatttc tttggtgtcg gagatgtcac tgatcttgaa aatgcattag ttggctgtct
     2281 acacgacttt gagcacatag aagaagcact atcagaatat gatttatatc attatttcgg
     2341 tgacatagat agacatgaat taattagatt aatgtcataa acgattaaag gactattctt
     2401 acatgtatga gatgttaaga atagtccttt tatgattgcg ttatggaatt cgaatcaatg
     2461 ttattcaccg agaaatgcct ccaaattttt gtattctgcc gtatattgat taaatgctgc
     2521 ttcatcttta ttgacgagtg cgtcatctat aagttctatg agctgtcgtt ttcttacctc
     2581 tcgaagtgct tcattgataa ttaattcaat acccaaatca ttgacagttg tgacaaacgc
     2641 ttctaaagtt gtatgtttaa cattagtatt gtatttcata gaaaccacta cctttcttta
     2701 atttataata agtatacatt tttctaattt tgaaatcaat gaatattct
//
LOCUS       DALAAR010000165         2745 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000165 DALAAR010000000
VERSION     DALAAR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.172"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(150..1004)
                     /locus_tag="O2458_002789"
     CDS             complement(150..1004)
                     /locus_tag="O2458_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ3888611.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1280..1504)
                     /locus_tag="O2458_002790"
     CDS             complement(1280..1504)
                     /locus_tag="O2458_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888612.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            1703..2173
                     /gene="sigS"
                     /locus_tag="O2458_002791"
     CDS             1703..2173
                     /gene="sigS"
                     /locus_tag="O2458_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCZ3888613.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            2286..2729
                     /locus_tag="O2458_002792"
     CDS             2286..2729
                     /locus_tag="O2458_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCZ3888614.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
BASE COUNT          928 a          473 c          322 g         1022 t
ORIGIN      
        1 ttctttatcg atgtcctgcc ccacttgcat tggtgggatt atcaatcaat gtgttttctc
       61 taatgcaaaa agacgctttc tatcaaaaca agatagaaag cgtcttttta gtatgtccga
      121 gtcataaaaa caactcgtag cttatcaaat tacttattca aatgtttact gtcatcttta
      181 tacacaaagt atttgaagta ttttccttca gtcttcatgt tcttataaaa gtcagcgata
      241 attgttgcat tactttctgc ccacttaata tctgtagcat attgatgttc tcctggattt
      301 tttggattcc atctcatact atacaatgta ttttgatctg tgcttgataa gaagtgctta
      361 tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg tatcccaacc atgcttttta
      421 gcatattctg cacctgtttt aattgggtct ttatcaaggg ctcctactcc atagaaattg
      481 tagtactttt tgccatcaat ttcgactcca ttagctaatt cacttttaac tgcgccagtt
      541 tctaataatg catgtgaaat taaataaact tcgttaacgt gcttatcttt agcagctttt
      601 aagaaatcat ccgtatgttt caataacgtt ggtctatcta ctaacatacg tttaattcta
      661 tttttatcaa tcccttgata ctttgataaa tctaaaaatt gatatttttg cttttcatta
      721 tcgataaaag taccgctatc cattgcactt ttaatttcag ttgcagatgc atctctccat
      781 gcatcattct ttttatttga tacctgttga ctcgtataat tatttatttg tttctttgct
      841 gcatcgttta atgtaacatt taacttttca atcttaatgt cggatttaac atgtttgaaa
      901 aatatctgat cggatatcat tgagaaaaat aaaaatgaga caacagcaaa tatgacaaca
      961 agtcctatta ttccaaaaat agaacctttc ttgtgtttat tcatatccac acctcttagg
     1021 tcattgttgt tatacattca gtttgactac gataataaag tttaacatta ttaaaaccgt
     1081 ttaacaattt tatctatgca ttgttacata ttcataaata aacagtaatg taaatgaaac
     1141 gttttaaacc atttatcaac attactttac gttgatctac gaatcataaa tgttttatct
     1201 ttttgtataa cctttgaaaa cctttatatc aataatctta ttattcacgt attaaaaaac
     1261 aaaaaattct tttgttcgtt tacttagttt cttttttata atccatcata ataaaacttg
     1321 ctgcatttga tctattttta taatagtact gatttaaatc aatcgtgcct tcaatttgac
     1381 catcacgata catcatttca ttagtgaagt tatttatcat cacgaaatca gcgcgatctc
     1441 ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc taaatttaaa tgtgcgtaat
     1501 ccattgttac ctcccgatta caaattttgt tctatctact atttagcaaa gtaataatac
     1561 atcatatcac attaaattaa ttacttatgt cttatattta acacttaatt tagcgattac
     1621 gcaaatgaat gatttttcta aatattttat tctaacttta tccggaaata tttgttatac
     1681 gtataacttg gagaatgact acttgaaatt taatgacgta tacaacaaac accacaaaat
     1741 catacaccat cttttaaaaa aatataatat tagctataat tatgatgagt attatcaact
     1801 actcttgata aaaatgtggc aattgagtca gatatataaa ccctcaagca agcaatcttt
     1861 atcctctttt ttattcactc gattaaattt ttaccttatc gatttattca gacaacaaaa
     1921 tcaattaaaa gatgtcattt tatgtgagaa taattcacca acattaactg aacaaccaac
     1981 atactttaat gaacatgacc ttcgtttaca agatatcttc aagcttttaa atcaaagaga
     2041 aagactatgg ctcaaactat accttgaagg atacaagcaa tttgaaattg ctgaaatcat
     2101 gtcattatcg ctttcaacga ttaaattaat taagatgtcc gttaagcgta aatgccaaca
     2161 taattttaat tagtttattg tgaaatttcg gtcgatgtgt taatgatgtt gaaacaccct
     2221 aacatcttca tataaattta ttttaaccat taaattaaca acttattaaa cgaggagtac
     2281 tatatttgca agacaattct actaaatatc tactttatat ccaaactgct acttcaaacc
     2341 atctcgaaac aaattgtgtc tttttacatt gcgattacat tcttaaagtt ccaattaaca
     2401 aactcgtttc atattatgcg aaattgcatt tatcatcaca aagtgtgcta attgagactg
     2461 caaaaaacat actaaatatt aataaactgg ttcctattta catcaaccca aaaactatac
     2521 tttttccgtt aaaacataaa cgtgcaccga tacaaattta tatcaatgca cattatattg
     2581 ttggtatgac tgccatagag aattcaacat taatacattt tcaagaaggc attcagctag
     2641 aagttgatga gccattttct ctagtttcaa aaaaatgtca tgaaagttta gctttgaagc
     2701 acttcattga aaatactatt tcgaattaaa tttaatttct aaaag
//
LOCUS       DALAAR010000166         2705 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000166 DALAAR010000000
VERSION     DALAAR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.173"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..323
                     /locus_tag="O2458_002793"
     CDS             <1..323
                     /locus_tag="O2458_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ3888615.1"
                     /translation="IFLKMKYDEKKYYDEQKERITIYMKYNVKGYKNISFANFKENPM
                     DGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDELGRKFNKNPKSVSEIKKEQN
                     TSNK"
     gene            517..693
                     /locus_tag="O2458_002794"
                     /pseudo
     CDS             517..693
                     /locus_tag="O2458_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(671..772)
                     /locus_tag="O2458_002795"
     CDS             complement(671..772)
                     /locus_tag="O2458_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888616.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(895..990)
                     /gene="pepA1"
                     /locus_tag="O2458_002796"
     CDS             complement(895..990)
                     /gene="pepA1"
                     /locus_tag="O2458_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCZ3888617.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1193..1336
                     /locus_tag="O2458_002797"
     CDS             1193..1336
                     /locus_tag="O2458_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ3888618.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1940..2323
                     /locus_tag="O2458_002798"
     CDS             1940..2323
                     /locus_tag="O2458_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888619.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            2334..2510
                     /locus_tag="O2458_002799"
     CDS             2334..2510
                     /locus_tag="O2458_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888620.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            2512..2697
                     /locus_tag="O2458_002800"
     CDS             2512..2697
                     /locus_tag="O2458_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888621.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT         1041 a          345 c          461 g          858 t
ORIGIN      
        1 gaatcttttt gaagatgaaa tatgacgaaa agaaatatta tgatgaacaa aaagaaagaa
       61 taacgattta tatgaagtac aatgtgaaag gttataaaaa tataagcttc gctaatttta
      121 aagaaaaccc aatggatggt tattctatta gtggttatat aaataatgat aaaaagttat
      181 catttacagc tggtataaga tctgttgatg attttcaatt tgataccgat atttcttata
      241 cagatgaatt gggtagaaaa tttaataaaa atcctaagtc agtttctgaa ataaaaaaag
      301 agcaaaatac gtccaataaa taattgttca tattgtgatg aaacaaaaat ataagtcatt
      361 agatgagttt aactatgtta taaatatttg tagtatctat aaaaatctcg acactattaa
      421 aatgataaag tgccgaggtt ttcttactta tttagttaat tcaaagttta tgccagattc
      481 ataagaattt gtgacacttt taatagtgta accattgatt attacaattt atcaaatggt
      541 cctttagaag gtataaataa caaaataaaa ttaattaaac gtacatcttt tggttatgga
      601 agttacaatc atttgcgcaa tcgaatatta ttatgttcaa aactttacgc tccaaaaagt
      661 aaaaaggaag ttaagcaatg tttagttgct taacttcgga tattgaacgc atcagtccaa
      721 tttgacatag agcctttttt agttcttgat gtttctcttt aaaaccttgc atattttaca
      781 aaaagaaaaa ttagcagtat aattaagaca acgaaaataa gtatttactt atacaccaat
      841 cccctcacta tttgcggtag tgaggggatt tttattggtg cggctatatg tcacctattt
      901 tgtattgcgt ctacttagcc aataagaaaa aaacgcaatg gcacagccac tgatgactgg
      961 tgctatgatg tgaacgaaaa taagcatcac cttatacacc tcctctctgc gtctaaattg
     1021 acgcctgaga gataggcgac tctactatta tatcatcggc aaatatacaa gcacagtcac
     1081 ttgcttctga taagttatat gattctagct gatagattga atcgtctaca cttaattgga
     1141 caaattctat gagaatagat attgttaatt taagaaagta ggcgatttta ttatgacaag
     1201 agaaagaaga tcatttagtt cagagtttaa gttacaaatg gttagattat ataaaaatgg
     1261 taagcctagg aatgaaatta tacgcgagta tgatttcaca ccttcgacgt ttgtaaatgg
     1321 cggttataaa atgtaggaaa atggataaag caacatcaaa acacgggtac attcaatcac
     1381 caagataact tatcggatga agaaaaagag ctgattaaat tacgcaaaga agttcaacat
     1441 ttaaaaatgg agaacgatat tttaaagcaa gtagcgctga ttatggggca aaaatagaag
     1501 tcattcaaaa gaatgcacat caatattcag tatcagcaat gtgtaaagtc ctgataatac
     1561 taagaagtac ctattatgat tctataaaaa gaaaagataa taaaatcact aaagatgatt
     1621 caaacataga acatgccgtc ataaatattt ttaattctaa tagaaaagtc tttggtacaa
     1681 gacgaattaa aaatcattta aatgacaagg gtctcactgt atctggacaa aagataggtc
     1741 gatcatgaaa aaatctagtt tctgtttata cgaaatctaa atacaaaaat catctaaaag
     1801 aaactaatga aaaacgaatt aaaaatcttt attattagct gctggtgtat tatttgttag
     1861 tccaatttca ttatcttata attcagatgt agctcatgct gaagataagt tagaccattc
     1921 tcaagcaaag gtaatatatt tgagtaacca aaatttattt gatgaacttg agaaaaaagg
     1981 ttataaactg gaagatatat ttacaaaaga agaaataaaa aaatataaag ctgaagacca
     2041 attgagagcg ggtaaaactc aatatgtaga aacaggtaaa gatactgcaa cattatatct
     2101 ttcttctgca tatacaaaaa caatagctgc tttaggtgcg ggggctatta gcgttatttc
     2161 tgcactaaca ggaggtttag ttggcgcagg tgttggtggt ttccttggat ctatagctgc
     2221 atcgaatatt gatactagta agggaatata tataaaatta aaaactaaaa agaatgcagc
     2281 cggggaatac gttctgacag gagaaaaatg gggatatcag taagggtgat tttatggata
     2341 gattaaaata ttcacttaaa gttggaattt tagcattatt attattttgt actttaaatt
     2401 atttagttcc aatgcaaagc aatgcttttt caataattat atattcggca atttttgctg
     2461 tgttacttat gcttttagtt tatatatttt taggaatttt aaagaaatga catgaaacaa
     2521 attagcattg gctatgaaga aatctatggg gatagaattt ttcatagcca ttttttaaaa
     2581 gagcatgaag taaaaaattg gaaaaccgtc aatcaaataa ttgaaaaaga acacttggac
     2641 aaaaatgaat atgaagcgga accgccgtta tttttctttt caggatcgtt agattagatt
     2701 cggtt
//
LOCUS       DALAAR010000167         2659 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000167 DALAAR010000000
VERSION     DALAAR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.174"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(78..269)
                     /locus_tag="O2458_002801"
     CDS             complement(78..269)
                     /locus_tag="O2458_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888622.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNVKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O2458_002802"
     CDS             430..759
                     /locus_tag="O2458_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888623.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O2458_002803"
     CDS             772..1233
                     /locus_tag="O2458_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ3888624.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O2458_002804"
     CDS             1251..1685
                     /locus_tag="O2458_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888625.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O2458_002805"
     CDS             1714..2109
                     /locus_tag="O2458_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888626.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O2458_002806"
     CDS             2199..2345
                     /locus_tag="O2458_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888627.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..>2659)
                     /locus_tag="O2458_002807"
     CDS             complement(2342..>2659)
                     /locus_tag="O2458_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ3888628.1"
                     /translation="IIWLINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENR
                     IIEEGELKTYNSAGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLM
                     NEY"
BASE COUNT         1066 a          403 c          420 g          770 t
ORIGIN      
        1 ttcctccttt taatttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttacattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataat
//
LOCUS       DALAAR010000168         2603 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000168 DALAAR010000000
VERSION     DALAAR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.175"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(87..1223)
                     /locus_tag="O2458_002808"
     CDS             complement(87..1223)
                     /locus_tag="O2458_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCZ3888629.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1255..1884)
                     /locus_tag="O2458_002809"
     CDS             complement(1255..1884)
                     /locus_tag="O2458_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCZ3888630.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="O2458_002810"
     CDS             complement(1903..2172)
                     /locus_tag="O2458_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCZ3888631.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..>2603
                     /locus_tag="O2458_002811"
     CDS             2335..>2603
                     /locus_tag="O2458_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCZ3888632.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVL"
BASE COUNT          774 a          411 c          406 g         1012 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga tttacaaata
     2581 ttttatagtt tcattgtttt aca
//
LOCUS       DALAAR010000169         2583 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000169 DALAAR010000000
VERSION     DALAAR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.176"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            177..992
                     /locus_tag="O2458_002812"
     CDS             177..992
                     /locus_tag="O2458_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCZ3888633.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1314..2492
                     /locus_tag="O2458_002813"
     CDS             1314..2492
                     /locus_tag="O2458_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCZ3888634.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
BASE COUNT          952 a          356 c          447 g          828 t
ORIGIN      
        1 catgataaat tagtggttag ctatattttt ttactttgca acagaaccta taaaaaacat
       61 gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt caaaaattag
      121 gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag gttaaaatgc
      181 attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa gtgaatgata
      241 ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat ttagatcgtt
      301 atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga tatgatcaaa
      361 gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat gaaattgtcg
      421 aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa gtttacttaa
      481 tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat ccaaatacag
      541 tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta tttggcaatc
      601 ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag ggggtatgtt
      661 ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa caaatctcta
      721 tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat gctcaacaat
      781 ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc tatgtagatt
      841 ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc tatgatcgtt
      901 tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac gaagttattg
      961 aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt atttgattat
     1021 aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct atgacgatag
     1081 attttttcat agctattttt tatagttata gagaggagta gactgtccag actcttggat
     1141 tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt cgtttgtagt
     1201 atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa gtaaagtttt
     1261 tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt gatattagta
     1321 aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag tttttcatcc
     1381 aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta gaccacccac
     1441 aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt tgttgtgtaa
     1501 atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg agcgctctaa
     1561 gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga ccgacgctca
     1621 aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga gaacgtgccc
     1681 gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt cttgaattac
     1741 tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca atcattgcac
     1801 tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac aaggatgtac
     1861 ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa gctacaaaat
     1921 atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat agacattcct
     1981 ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt catcatctca
     2041 aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt gaaaatattc
     2101 attcgatacc tggtattggt aagctaagca cagctatgat tattggggag attggtgata
     2161 ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat atcaaacgat
     2221 atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat aaaaaagcga
     2281 gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat tatgacaatc
     2341 atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct cataagactg
     2401 ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta atgaatcata
     2461 aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat atattttaat
     2521 accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt taattataag
     2581 tac
//
LOCUS       DALAAR010000170         2570 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000170 DALAAR010000000
VERSION     DALAAR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.177"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            106..819
                     /locus_tag="O2458_002814"
     CDS             106..819
                     /locus_tag="O2458_002814"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCZ3888635.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            complement(1259..1894)
                     /gene="sdpA"
                     /locus_tag="O2458_002815"
     CDS             complement(1259..1894)
                     /gene="sdpA"
                     /locus_tag="O2458_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCZ3888636.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            2190..>2570
                     /locus_tag="O2458_002816"
     CDS             2190..>2570
                     /locus_tag="O2458_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888637.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYF"
BASE COUNT          985 a          323 c          415 g          847 t
ORIGIN      
        1 gttttatata acaaagaaat aaaaatcatt ttttacaaat ggttgtaaga aaaagacatg
       61 cagatgttgt taaaatttta ataagaatca aggaggctat attatatggc taaactaaat
      121 atagaagtat ttgcggacgg tgcagatatt gaagaaatga aagcagctta taaaaacaaa
      181 caagtggatg gttttacaac aaatcctagt ttaatggcta aagcgggcgt aacagattac
      241 aaagcttttg ctgaagaagc tgtgaaagaa attccagatg cttcaatttc atttgaagta
      301 tttgcagacg atttagaaac tatggaaaaa gaagcagcaa ttttaaaaca atatggcgaa
      361 aatgtatttg ttaaaattcc tattgtaaat acaaaaggtg aatcaacgat tcctttaatt
      421 aaaaaacttt cagctgacaa tgtgagatta aacgttacgg ctgtttacac aattgaacaa
      481 gttaaagaaa taactgaagc agtaactgaa ggtgtgccaa catatgtttc agtatttgca
      541 ggacgtattg cagatacagg cgtagatcca ttaccattaa tgaaagaggc tgtaaaagtt
      601 acgcatagta aagacggcgt taaattatta tgggcaagtt gccgcgaatt attcaatgtg
      661 attcaagctg atgaaattgg tgcagatatt attacatgcc cagcagatgt tgtgaaaaaa
      721 gtgaatacaa acttaggtcg cgatattaac gaattatcag tagatacagt taaaggcttt
      781 gcgaaagata ttcaatcttc aggtctttct attctataat atataaatat atttaaaccc
      841 tcataggcat tgtgcttatg ggggtttttc catcatagtt attaatgaag aagaagtcaa
      901 aattcagcta agtaaaagct cgagttataa aaaagtctaa attgattcaa caggagtaga
      961 aaaggaaatt agtcttgcat attttagaaa atgaataaaa acagttacta tatgcaactc
     1021 aagaattaaa tattatttaa gccacatacc tattagagta caaaatatac aagttgtact
     1081 tatatctagg tatgtgactt ttgattttaa gcgtaaagta aaagttttta tgtttgtatt
     1141 agttctaaat tattttgcac ttgatgacac gtatttttga gtttaagtgg tgactcaagt
     1201 tcaataaaat aaatatcaga tagataattt gaaaataata tgaaaggtta tctcaaaatt
     1261 atctccatat tataaagagc agagctaaca agttatttaa gaagtgcatg aatattacaa
     1321 cttctaatcg ttttgttttt atataaatta ggccaaaaat caaaccagaa tataagtaag
     1381 gtaaataacc aatccaagtg tcagattcgt gtaatgatgc aaaaactaaa ctagacacaa
     1441 ttaaccctaa aaacaagtgt tttctgaaga taacccttat tatcataccg cgaaaaacaa
     1501 tttcttccac aatagcgggg atgatggtaa cagtgaaaat agaaatatag aatggtgtat
     1561 ttcgtattgc ttgctctaat tgttgttcat ttgccggtac gtcatgaaaa tataaataaa
     1621 gattatctaa cccataaatt aatagtgcac cgcctataat ataaataact tgagacaatg
     1681 ttaatttttt aaaagagaat aaattgattt tgaatagcct caatagaatt aaacctacta
     1741 atacaattag catgctataa agtaaatgta cttcactgac aactatgccg ataaataaaa
     1801 tgatagtaat aaaaaatgct gccattaaag ttaaaaaata atttcctcgt gtaaccgata
     1861 aatcatcgta aaaaaagtct ttgaattttt gcatgctaaa tcctttcttt tcatttttta
     1921 aaattatttt gaatgcaata tgattaaggc tagattcata atatatgaaa catatttacg
     1981 attaaaattt atcattgtac ctacctaaaa agcattagtt tgaaaaatta gcagactgta
     2041 ttttagcgtc aaatcaccat aaatgaaatt gtaatatgga tttttgtaga tttgatacat
     2101 atatttctgt aaaatatata aaatacactt tcaattgacg ctaatgttta aaattacgtc
     2161 aatattataa aggagagaaa tccttaatta tgaataaagc atcatttgat aaaaaagtta
     2221 aaaagcaatt atggtttttg aacaagaagg agaaacaagc attagatcaa cgattgtctt
     2281 ctatatctga tgatgacagc gttaacttaa ataagcctgt tacctttgct aatgcttact
     2341 taagacaaaa tgtatttcga aataaagaaa caaaaagtta tagtatgttt gttacattag
     2401 tagtgatgat gtttgcttat gttgctttat taggtttgtt tttatttggg ctaattacaa
     2461 gtctatctgg cgtacaattt ttcgttagtc ctaaggtaga tttatctaca actgttgtta
     2521 ttttaacaat tattggagcg attttattaa tgtttgctag catatatttt
//
LOCUS       DALAAR010000171         2515 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000171 DALAAR010000000
VERSION     DALAAR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.178"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..438)
                     /locus_tag="O2458_002817"
     CDS             complement(<1..438)
                     /locus_tag="O2458_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3888638.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSL"
     gene            complement(562..1191)
                     /locus_tag="O2458_002818"
     CDS             complement(562..1191)
                     /locus_tag="O2458_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ3888639.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1644..2273)
                     /locus_tag="O2458_002819"
                     /pseudo
     CDS             complement(1644..2273)
                     /locus_tag="O2458_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(2320..>2515)
                     /locus_tag="O2458_002820"
     CDS             complement(2320..>2515)
                     /locus_tag="O2458_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3888640.1"
                     /translation="ICFTIFYYLFTGFMSYGAFYLLVFENVQNLILYVSWLFMTMLFM
                     FMNMHSIIDKKVHIFLKSNK"
BASE COUNT          946 a          404 c          269 g          896 t
ORIGIN      
        1 aagactcttt aaagtgggaa atatgattat cttattgctt ttatttttgt tttcattata
       61 gatttgtaac tttaactttt gatttaaata taaaaaaaac agaaaaatac ctagtattat
      121 gatgcaacaa attaaaatta ctatactata ttcatattta acgataaata aatgggaata
      181 ccttcttaac ataacaccaa ataagaccgt acttcctgcc actggccaaa aagaattgat
      241 tttagctgtc ttcacaatgt ttaaagtttc aaaatctttt tcgctgattt tgacgtactt
      301 ttttggaatc agccaattaa tcattgggaa aaagagtgct aaccatgtac ttactaaatc
      361 aatcatcaaa tattcatctt tgtatttgat aattctatat ttgggattct tattaatatt
      421 ttcagtttca caaagcaatg tctccacttc ttttcaattg gttttatatt gatacacttt
      481 catatacatt agaagtcatt taaattctac tagcgtctta aaaggattgt gtcgacaaca
      541 tttagaatat tttaaatgta atcaattatt tttcattata acgcgaactt tttcattacc
      601 tatcgaagtc atatttaaca aaaagaaaag cattgtcatc ataatccaat aaataaatat
      661 tattatattt tgaggttcga ttgtcataag ggcaattaat gaaaatattg aaaaacttcc
      721 agcgaaaata tatccgaaaa ctaaaaaaca gccaagttta aacgttggta ttaatataac
      781 tctcttattt ttttcaatat ttgtatcaaa cacttttaac ttaagctttc tatttaagtt
      841 aaaataaaaa actataacac aaattaaagc gagagcacac aatatcatta caactagctt
      901 atctaattga acatcaaaaa catgtatgta tttccttaaa gtaactccaa acaaaaccga
      961 acttcctata actgaccaga aaacattatt tttatttggt ttaacaacat ttaaattttc
     1021 aaattcttct tcgctaatta tcgcgtactt ttttggaatg aaccaattaa tgaaaggaaa
     1081 aaagtacacg aaccaagtgc ttactaaatc gactaataag tattcaccgt tatgttgaat
     1141 gactttgtat ttcggatttt tataaatgac tctgacatcg caaagcaaca tttaccactc
     1201 ccgtttcatt agttttaaat gatccccaat actttttgta ccttcaccaa ctatcgttcc
     1261 aacactatct acagcttttt ctgtggtatt tttaaacagc gtctatttaa tgtcttatcc
     1321 aattagtttt atctcttaat ccaaatttat tagtatgaaa agtccaattt ttaagaattg
     1381 tctaaacaga ttccgcaaca aaaccagcta aaaccgtcca tactattgga ttaaaaacgc
     1441 caatgttata agagctatca tacaaaataa cacgctctat atttcatttt taaattaaaa
     1501 cagttttttc ctttatcaat cacaatgggc attttcactt ggtatattca aacattacta
     1561 tctattttcc tgatttaaga tatgattata tttttttgaa ccaccgttta aatatgcgat
     1621 actaccaatt tcgatcattt ttactactcg tttattctta aaataacatg aacattttta
     1681 ttacctatcg aagccatatt cactaaaaag aaaaacatcg tgataataac ccaagttaaa
     1741 aatattatta tattttgtga ctctgtcgtc aaaagaatgg ttaaagtaaa tattgaagcg
     1801 cttcctaaaa aaaagtacgc aaaaactatt aacaatcctt gtttgaaagt cggtattaat
     1861 acaactctat tcttattaac cacgttggta ttaaaaattt ttaatgttaa ttttttattt
     1921 agataaaaat aaaaaattaa catccctatt aacatgataa aaaatacagt gattgctagt
     1981 tgtttttcaa actgaacatt aaagaagtta ccgtactttc tcaatataat tccaaataga
     2041 actgaacttc ctgcaaccgg ccaaaaaaca ttatttttaa caggctcgac tatatttaac
     2101 ctttaaaatt cattttcgct aatttttgcg tattttttgg gcaaaaacca attaatcata
     2161 gggaaaaaat aagtaatcca agtacttact aaatcgacca tgaaatattc attattatat
     2221 ctaataactc gatatttagg atttttataa atttgtctag attcacaaag cactgtttac
     2281 cacccctaat taacaattga aaactaacta aatttgtaac tatttattag actttaagaa
     2341 tatatgtact tttttatcta taattgaatg catattcata aacataaata gcatagtcat
     2401 gaaaagccaa gaaacatata agattaaatt ttgcacattt tcaaatacca acaagtaaaa
     2461 tgccccataa gacatgaaac cagtaaatag gtaataaaaa attgtgaaac aaata
//
LOCUS       DALAAR010000172         2437 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000172 DALAAR010000000
VERSION     DALAAR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.179"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            55..1275
                     /locus_tag="O2458_002821"
     CDS             55..1275
                     /locus_tag="O2458_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCZ3888641.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
     gene            1689..>2437
                     /locus_tag="O2458_002822"
     CDS             1689..>2437
                     /locus_tag="O2458_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCZ3888642.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
BASE COUNT          959 a          250 c          415 g          813 t
ORIGIN      
        1 aaaattgatt tattggacaa aaaatacatt ttaaatgagt agaggaggct gcaaatgggt
       61 tacagaactt taaaaagtat ttttcatgaa cacaatgaaa gtaaaatgaa ggaagagtat
      121 attaaaagat ttaattcttt agcttctttc aatactaata ttaatatcat acctatggaa
      181 aatggaaaaa aagttaatga tttggaatat cctctattct ttatggtgac taaaaatcta
      241 tcaaaaaaac aagaattaat atcaattaat agtagaaaaa ttgatagagc acttaattct
      301 ttaccatatg cagctagaga acaatatttt aatgatttat taatcgatga attacaaagt
      361 actaatgaaa ttgaaaatgt atttagtact aaacaagaga ttgcacatgc gttaaataac
      421 caagcatcag aatttcttaa gttcagaggc ctcgtggatc aatataaaga gatagaactt
      481 aataaaaaaa ttaaagttga taatgtaaga gacattagag cgatttatga taaattagtt
      541 tcaaatgaaa ttaacgaaca agataagtta gatggagagc tatttcgtaa aaattttgtc
      601 ggtgtgcatg atgggtcaac gaataaatat atacatgttg ggttacaacc tgaaaccaaa
      661 attgttgaat ttataggtga aatgctaaca tttttaaaat attttgatgc gcctcagccg
      721 ttcaaaatca tggctagtca ttatctgttt gaatatatac atccttttta tgatggcaat
      781 ggtagagttg gaagatttat aattgccaag ctcttgagtg attattatga taattatact
      841 gctttgacat tttcatatgt tataaataga aataaatcga aatattacaa ggcgtttatg
      901 actgcttcaa atcatttgaa ttgtggtgat ttgacggaat ttattgacac aatgcttgag
      961 ctattgatag caggacaaga aagaatactt gatgagttaa taccaaaaat ggatgcgact
     1021 gaaaaattaa cgctttattt aactagtcat tataaacaga tagattatga atttttgtat
     1081 ctcctttcaa tggataaatt gtttggaaat aaaagaaata gattaacatt aattgattta
     1141 gaaaacatat tgggagtagg aagagtaaaa attaataata caattaaaaa atatgataac
     1201 tacttagtta aaattaaaag tagaccaaca atttatgaga taagtgatga atttctaaat
     1261 tcgattataa aataaatata atatgtgatc agctatactt atagtatttg taagatcgcg
     1321 taattgcttc attgaaaaaa tatatttgtt gagagtggtg catgacaaat gtactgctct
     1381 ttttgttgtg tatcagtatt gtgatgtttt aatgagaata ttatatgaat cattatgaaa
     1441 tttaataaaa ataaaagaaa tgattatcat tttttcttat atactgttaa acggtttgga
     1501 atttttaggt atacactgta ttggttgata taactcaact aataattgcg aacagagtat
     1561 ttcaaattga aaagtattat gagcgtgata cataatcaaa attgtaggct caagaaccac
     1621 tacataataa accataagcg gttctttatc atttatgtct cgctctcaaa tgtaaattaa
     1681 taattgtttt gggggagttt gaagttaaat atttaacagg atttatttta atattattgt
     1741 tagaaggaat ttttacaaat tcagcgagtg caatcgaata ttcagactta catcataaaa
     1801 gtaagtttga ttcaaagcgt ctaagtaatg ctaagatgtc attcatcaat ccaactcagc
     1861 ttgaaaataa aaacacaaac gatagactgt tgaagcatga tttgttattt catgacatgt
     1921 tcgtaaatga tgattggaaa aaggatttta aagttgaatt tgaaaatgag gcactttcaa
     1981 agaaatttat aaataaggat atcgatatat ttgctggaaa ttatggatac ggatgtcatg
     2041 ggggagcaac caataaaacg caatgtagtt atggtggtgt tactttaagt gacaataata
     2101 aatacgatga ttataagaat ataccttgta atttatggat tgacggacat caaacagaaa
     2161 tagaactaac tgcagtaaaa acgaaaaaga aaattgttac tattcaagaa ttagaggttc
     2221 aattaagaaa ttatttgaat gagaagtata agttgtacga acaaggtggc gacattgtta
     2281 aagggtatgt taaatattat aatgatgatg aacaaaatgt agaatatgat ttttataatt
     2341 taaatggtga gtatggtcgt gaggtattaa aaatgtatgc tgataataaa actatcaata
     2401 gtgacaaatt gcatttagat atctatttat tcaaatc
//
LOCUS       DALAAR010000173         2250 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000173 DALAAR010000000
VERSION     DALAAR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.180"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            107..229
                     /locus_tag="O2458_002823"
     CDS             107..229
                     /locus_tag="O2458_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888643.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            258..476
                     /locus_tag="O2458_002824"
     CDS             258..476
                     /locus_tag="O2458_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCZ3888644.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            476..2173
                     /locus_tag="O2458_002825"
     CDS             476..2173
                     /locus_tag="O2458_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ3888645.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT          797 a          347 c          380 g          726 t
ORIGIN      
        1 cgaaatactt agatttctag tgagataaac tgctttattt attatcattc atcatgtaaa
       61 ataagattta actgaaattt tagtgttatt tcactaattt tttaaaatga acgacatgat
      121 gaacctagtt attaaccaaa tcgttattaa gttacattat agagatgatt ggaatgaatt
      181 tatcgatata tactccaata cgattttact agggttaaca ataaattaaa caaacattct
      241 taggaggaat ttttaacatg gcagtattta aagtttttta tcaacataac agagacgagg
      301 taattgtgcg tgaaaataca caatcacttt atgttgaagc tcaaacagaa gaacaagtac
      361 gtcgttactt gaaagatcgt aattttaata tcgaatttat cactaaatta gagggcgcac
      421 atttagatta cgaaaaagaa aactcagaac actttaatgt ggagattgct aaataatgaa
      481 acaattacat ccaaatgaag taggtgtata tgcacttgga ggtctaggtg aaatcggtaa
      541 aaatacttat gcagttgagt ataaagacga aattgtcatt atcgatgcag gtatcaaatt
      601 ccctgatgat aacttattag ggattgatta tgttatacct gactacacat atctagttca
      661 aaaccaagat aaaattgttg gcctatttat aacacatggt cacgaagacc atataggcgg
      721 tgtgcccttc ctattaaaac aacttaatat acctatttat ggtggtcctt tagcattagg
      781 tttaatccgt aataaacttg aagaacatca tttattacgt actgctaaac taaatgaaat
      841 caatgaggac agtgtgatta aatctaagca ctttacgatt tctttctact taactacaca
      901 tagtattcct gaaacttatg gcgtcatcgt agatacacct gaaggaaaag tagttcatac
      961 cggtgacttt aaatttgatt ttacacctgt aggcaaacca gcaaacattg ctaaaatggc
     1021 tcaattaggc gaagaaggcg ttctatgttt actttcagac tcaacaaatt cacttgtgcc
     1081 tgattttact ttaagcgaac gtgaagttgg tcaaaacgta gataagatct tccgtaattg
     1141 taaaggtcgt attatatttg ctaccttcgc ttctaatatt taccgagttc aacaagcagt
     1201 tgaagctgct atcaaaaata accgtaaaat tgttacgttc ggtcgttcga tggaaaacaa
     1261 tattaaaata ggtatggaac ttggttatat taaagcacca cctgaaacat ttattgaacc
     1321 taataaaatt aataccgtac cgaagcatga gttattgata ctatgtactg gttcacaagg
     1381 tgaaccaatg gcagcattat ctagaattgc taatggtact cataagcaaa ttaaaattat
     1441 acctgaagat accgttgtat ttagttcatc acctatccca ggtaatacaa aaagtattaa
     1501 cagaactatt aattccttgt ataaagctgg tgcagatgtt atccatagca agatttctaa
     1561 catccatact tcagggcatg gttctcaagg tgatcaacaa ttaatgcttc gattaatcaa
     1621 gccgaaatat ttcttaccta ttcatggtga ataccgtatg ttaaaagcac atggtgagac
     1681 tggtgttgaa tgcggcgttg aagaagataa tgtcttcatc tttgatattg gagatgtctt
     1741 agctttaaca cacgattcag cacgtaaagc tggtcgcatt ccatctggta atgtacttgt
     1801 tgatggtagt ggtatcggtg atatcggtaa tgttgtaata agagaccgta agctattatc
     1861 tgaagaaggt ttagttatcg ttgttgttag tattgatttt aatacaaata aattactttc
     1921 tggtccagac attatttctc gaggatttgt atatatgagg gaatcaggtc aattaattta
     1981 tgatgcacaa cgcaaaatca aaactgatgt tattagtaag ttaaatcaaa ataaagatat
     2041 tcaatggcat cagattaaat cttctatcat tgaaacatta caaccttatt tatttgaaaa
     2101 aacagctaga aaaccaatga ttttaccagt cattatgaag gtaaacgaac aaaaagaatc
     2161 aaacaataaa taatcaaaaa gctactaact ttgaagtgaa gttttaatta aactcaccac
     2221 ccattgttag tagctttttc tttatatatg
//
LOCUS       DALAAR010000174         2200 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000174 DALAAR010000000
VERSION     DALAAR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.181"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            78..527
                     /locus_tag="O2458_002826"
     CDS             78..527
                     /locus_tag="O2458_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCZ3888646.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(622..957)
                     /locus_tag="O2458_002827"
                     /pseudo
     CDS             complement(622..957)
                     /locus_tag="O2458_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1607..2098)
                     /gene="sak"
                     /locus_tag="O2458_002828"
     CDS             complement(1607..2098)
                     /gene="sak"
                     /locus_tag="O2458_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCZ3888647.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
BASE COUNT          778 a          371 c          322 g          729 t
ORIGIN      
        1 agattaaact cattttagga atttcgcaaa actaactata aatttaaata tagaatttaa
       61 ggagaattaa catcattatg aaaaagaaat tagcaacaac agttttagca ttaagttttt
      121 taacggcagg aatcagtaca caccatcatt cagcgaaagc ttttactttt gaaccgtttc
      181 ctacaaatga agaaatagaa tcaaataaga aaatgttaga gaaagaaaaa gcttataaag
      241 aatcatttaa aaatagtggt cttcctacaa cgctaggaaa attagatgaa cgtttgagaa
      301 attatttaaa gaaaggcaca aaaaattctg ctcaatttga aaaaatggtt attttaactg
      361 aaaataaagg ttactataca gtatatctga atacaccact tgctgaagat agaaaaaatg
      421 ttgagttact aggtaaaatg tataaaacat acttctttaa aaaaggagag tctaaatcat
      481 cttatgtaat taatggtcct ggaaaaacta atgaatatgc atactaatag tagttacata
      541 aattaaaagg tagatatttc ttttttatat aaaggtttgg cagacatttc ataacttgcc
      601 aaacctttat atatctaatt atcaaactgc actaaactta ccaaaactgc ttattctatt
      661 acctgccttg tctacctctc ctgtcgctat ataacgacgt tgtccactat tagcaatata
      721 agtaatccat ctatagccat taatgcaata tgcgccgtca tatttgatag ttgcgttatt
      781 gggtaataca cctgtaattc ttgaattagt tgaatagcca tccctcacgt tattaccttt
      841 aacattggca actgtgtaat aaccagtctc ttttttatac ggtacattgt ttttatcgag
      901 tgtataacct gctggcactg gtgaattctt ttcattttta gctggtgttt taacattact
      961 gatacctgat acacacttcc aataaaaata accacaccat ttaagatgcg gtgtagcgac
     1021 tgtaatattt ctatgttgtt gagatatatg tatcgagtga agggcaaaaa ggatatcaat
     1081 tgcaggataa gtgttaggtt actaggccac ttaacaggct atatagttca ctcctactat
     1141 atacagttaa ttataacata aaaagcaccc cgtaaactat tatacgggaa tgctaaagtc
     1201 atatatacta cgagggagta gtatgaaaac tatgctctct atcataagaa aaaacaccca
     1261 gtgacatgct tgggtgaaca aggatagatg taaatagttg atgcatgtgt aacacatcat
     1321 aacaaaaaac tagcccgaaa gctagctata acatacaatc taaaaagacg tcccttgaat
     1381 acgtctagaa agattataac ataaaaaaat aggcaagtac cgaagtacct gcctaaaaaa
     1441 ggattatcca ctttttcatc ctaactgatt tctccccata agtcacctaa tatctgatta
     1501 ggtggggaag aaccattcgt gcatgaatga gaatttgatg aaagataatt ttcactacac
     1561 atattcaatc aagacattgc tttctataat aaacaactat tttgttttat ttcttttcta
     1621 taataacctt tgtaattaag ttgaatccag ggtttttaat atgctctgat aaatctggga
     1681 caacaaaacc tttttctgtt atagggaaag acttcgtttc ttcttttttc ttattcttat
     1741 cataataagt gacttcgatc tttgcgcttg gatctaattc aactactcta aactctttat
     1801 atgctgtcgc atctaatgcc cattcgacat agtattcaat tttttctttt gtaagtgtag
     1861 tcccaggttt aataggaaac tcgacataat gaggggatag caattcattt cctttaccat
     1921 caactccagt cacatttacc atcaaatacg ggcctgttgg ttcaaaataa ctcgcgtcat
     1981 cgcctttttt atattttcct ttgtcgaatg aacttgatgc acttacctca ttagtaattg
     2041 aagaaaatga gaataataac aataaaacag ttaaaaataa taaacctctt ttgagcatgg
     2101 cgcttcctcc aaatataata tatttgtttt tatttatctc tttttatagc taacaccctt
     2161 atttaagatg ataaatatca acaacttcat tttatattga
//
LOCUS       DALAAR010000175         2138 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000175 DALAAR010000000
VERSION     DALAAR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.182"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            61..1059
                     /gene="argF"
                     /locus_tag="O2458_002829"
     CDS             61..1059
                     /gene="argF"
                     /locus_tag="O2458_002829"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ3888648.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1079..2008
                     /gene="arcC"
                     /locus_tag="O2458_002830"
     CDS             1079..2008
                     /gene="arcC"
                     /locus_tag="O2458_002830"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ3888649.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
BASE COUNT          802 a          316 c          407 g          613 t
ORIGIN      
        1 gaaattggat tataaaaaaa taacatgaat aaaatattaa ttgaaaagga gatattcaaa
       61 atgaaaaatt taagaaatag aaacttttta actttgttag acttcacaca aaaagaaatg
      121 gaatttttac ttaatttatc tgaagatctt aaacgcgcaa aatatgcagg aatagaacaa
      181 caaaaaatga aaggtaaaaa tatcgctcta ctttttgaaa aagattcaac acgcactcga
      241 tgtgcatttg aaacagcggc ttatgatcaa ggtgcacatg taacatacct tgggccaaca
      301 ggttctcaaa tgggtaaaaa agagtctacc aaagatactg ctcgtgtttt aggtggaatg
      361 tatgatggca tagaatatcg tggattctca caaagagtag ttgaggattt ggcaaaatat
      421 tctggcgttc ccgtatggaa tggtttaaca gatgaagatc atcctacaca agtacttgct
      481 gattttttaa cagctaaaga agtattgaaa aaaccatata atgaaattaa cttcacatat
      541 gttggagatg gacgtaataa cgttgcaaat gccttaatgc aaggagcagc aatcatgggc
      601 atgactttcc atcttgtatg tcctaaagaa ctaaatccaa ctgatgaatt attaaatcgg
      661 tgtaatgata tagctgacaa aaacggtggt gaaatcctta taactgatga tattgatgaa
      721 ggtgtcaaag gatctgatgt aatttacaca gatgtttggg tatctatggg tgaacctgat
      781 gaagtttggg aaaaacgtat caaattatta gaaccatacc gtgtaactaa agaattaatg
      841 aaaaaaacag gtaatccaca tacaattttt gaacattgct taccatcatt ccatgatact
      901 gaaacaataa tcggtaaaca aattcaagaa aaatatggtc taactgaaat ggaagtaaca
      961 aatgaagtat ttgaaagtga acaatcagtt gttttccaag aagcagaaaa tagagcacat
     1021 acaataaaag cagtcatggt agcaacatta ggagagtaaa tgaaaggagg atataaatat
     1081 gtctaaaatc gtcgtagctt tgggtggtaa cgctttagga caatcgccta aagaacaatt
     1141 agatttatta aaaagtacat ctaaatctct agtaagttta atcgataaag gatacgaaat
     1201 tgtaattagt catggtaatg gtccacaagt tggaagtatt aacttaggtt taaattatgc
     1261 ggcggaacac aaacaaggtc ctccttttcc tttccctgaa tgtggtgcta tgagtcaagc
     1321 ttatattggc tatcagatgc aagaaagttt acaaaacgaa cttcattcaa tgggcataga
     1381 taaacaagtt gttacgctag taacacaagt tcaagttgca agcgatgatt ctgctttcaa
     1441 taaccctact aaaccaattg gattatttta tacaaaagag caggctgata aatttacgaa
     1501 agaaaaaggt tatacttttg tagaagattc tggacgcggc tatcgtcgcg ttgtcccttc
     1561 cccacaacct ataagtatag ttgagttaga tagtatagaa acattaatca ctcatggaac
     1621 actagttatc gctgccggcg gtggtggaat tccagtaatt aaggaaaatg aagtttatac
     1681 aggtgttgat gcagttattg ataaagataa aacgagtgct ttattagcag cacatttaca
     1741 atctgatcaa ttaatcatat taactgctgt ggaccatgtt tacattaact atggaaaaga
     1801 aaaccaaaga ggtctcgatg aagtgtctgt ggatgaaatg aaaaaacata tctctgatgg
     1861 tcaatttgct aaaggaagta tgcttccaaa agttgaagct gcacttcaat ttcttgaaaa
     1921 aaatactaaa ggcagtgttt tgattacatc tctagcagga ttaggggacg ctttagacgg
     1981 taaaatagga acattaatta agaattgaaa ttataatact ttagttcaat tacctttata
     2041 aacaaaagag cctataatcc tagggcactg aacccttaaa acgggaactt aataaaaaca
     2101 ccatgttcta ggctattaat tctctgtatt ttacaggg
//
LOCUS       DALAAR010000176         2127 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000176 DALAAR010000000
VERSION     DALAAR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.183"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            144..335
                     /locus_tag="O2458_002831"
     CDS             144..335
                     /locus_tag="O2458_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888650.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(474..659)
                     /locus_tag="O2458_002832"
     CDS             complement(474..659)
                     /locus_tag="O2458_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888651.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(595..876)
                     /locus_tag="O2458_002833"
     CDS             complement(595..876)
                     /locus_tag="O2458_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ3888652.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1165..1860
                     /locus_tag="O2458_002834"
     CDS             1165..1860
                     /locus_tag="O2458_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ3888653.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          745 a          314 c          384 g          684 t
ORIGIN      
        1 aaatagtgat aaatttgtga aatacatcac aaatcccttt atttatttgg aaattcatgt
       61 aatattagac ttgtaagaag ttaataaata gagagagacg agagagttta tataaatact
      121 atataaacat tggagtgatg attatgagaa aagagattga agcgcttatt ttctcagacg
      181 taagtagcta tgatatttac gtgaacactg gtgtaaatca aggattaatt ggtgacatca
      241 aagatggtta cctaactatt gattctatgc cttacattga tgctgagcgt ttgtatcact
      301 ttgctatgga acgtaaatcg ttagtcacta actagttctt attgccaatg attactaccc
      361 ctagtcggcg gcaattgaag tgtgattgat gtaacttgcc ctcgttggtg agcaattgag
      421 ggcagacccc tttaattaag taaaccctaa ctccccacaa atctggaacg atactaaaag
      481 ccacgtccta tattggatgt ggctttagtc atacttatat tatttttaaa acgattacct
      541 acaagattta catataaaat tctatcatgt ctgctatata taaacgaaat gatattagaa
      601 tccttcattt tcaacttgtt taaaattacg tttaatgccg ttataagaat atacaacatt
      661 atataatttt acttgggcgt catggacact tgagaagggc attcttacat cgtccatatt
      721 tttaacttcg tgtcgaaatg ctttttcagc tttttgtctt tctgcaagag acatacttag
      781 ccattcttct ttgctaaata aatcttcgaa tcgaaaatca aatgcggttc ttttactttc
      841 tgctcttaat gctaaaacac gctcatgata tgtcacgttt tattcctccc tctaatcatt
      901 caatcgaatg ataaatcaca gtattaactt tattactaat ataatgtttt tacaataaag
      961 aagtgacgat ttttcaacat ttttaaaaat aaaaaatata agcaacgatt aattgtatgt
     1021 ttcgaatggc ttgtaatgta atttatctat gaaaatgaag cgagttatta tgaccgttat
     1081 atgaattgtg atacgctttt taaaagtaat atgtatttat gatttaccct gaaaaaataa
     1141 aaagaatcaa aggagtcgat cattatgaca gtattaacag ataaagtagc agtagttaca
     1201 ggtgcaggta gtggtattgg agaagcaatt gcaacattac tacatgaaga aggggcaaaa
     1261 gttgtcttag caggtagaaa taaagaaaaa ttacaaaacg tagcgaatca attgtcacaa
     1321 gatagtgtga aggtagtgcc aacagatgta acgaataaag aagaagtcga tgaattgata
     1381 aaaattgcac aacaaacatt cggtggtttg gatattgtta tcaatagtgc ggggcaaatg
     1441 ttgtcgtcta agattactga ttatcaagta gatgagtggg atagtatgat tgatgtgaat
     1501 atcaaaggca ctttatatac ggcacaggct gcattaccaa ctatgttaga acaatcaagt
     1561 ggccatctta ttaacattgc atctatttct ggctttgaag taacgaaaag tagtacgatt
     1621 tatagtgcga cgaaagcagc agttcacact attactcaag gattagaaaa agagttggca
     1681 aagacaggcg ttaaagtaac aagcatttct ccaggaatgg tagatacagc cataactgcc
     1741 gcatacaatc caacagatcg taaaaaactt gaaccacaag atattgcaga agcagtatta
     1801 tatgcattaa cacaaccaaa gcacgttaac gtgaatgaaa ttacagtgcg tcctgtataa
     1861 aaaggtgttt gaggtctggg gtataggcat ttaatgtaat agctatcgat tttgttaatg
     1921 ttagtaaggc aataattaca tgagggcata ataggcaatt attccttgta caattctcag
     1981 taattgctga ttttaacaat caagttaatg aaggcgacga tgttagtgtt atggtttata
     2041 atctgtaatg ttcataatta tagattataa aaattttttc attttccacc acattttagg
     2101 agttacttta atgaacaaaa aacatgt
//
LOCUS       DALAAR010000177         2118 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000177 DALAAR010000000
VERSION     DALAAR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.184"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..567
                     /locus_tag="O2458_002835"
     CDS             <1..567
                     /locus_tag="O2458_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ3888654.1"
                     /translation="ERFPNYLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYA
                     RFLVVINVLTTITVVFALCNLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFT
                     LLKLPKVTKRWGGKTKTHFGLLLTALSSFIYIEKIIEYILVPNVVESKFIIIVSMLVI
                     AGAQFVAFQFIMQYSRFYIFELNTEDDD"
     gene            714..1325
                     /locus_tag="O2458_002836"
     CDS             714..1325
                     /locus_tag="O2458_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ3888655.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            1461..1679
                     /locus_tag="O2458_002837"
     CDS             1461..1679
                     /locus_tag="O2458_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888656.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1811..>2118
                     /locus_tag="O2458_002838"
     CDS             1811..>2118
                     /locus_tag="O2458_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888657.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     "
BASE COUNT          771 a          210 c          371 g          766 t
ORIGIN      
        1 gaaaggtttc caaattattt aaagataacg acaattatag aattaataat tatcataatt
       61 agtttacttc aatggattag atttatagat ttcgaaaaag aaagtgcaca gaaatataaa
      121 aaaatatatg ctcgattttt agttgttata aatgtgttaa ctactatcac tgtagtattt
      181 gcactgtgta atctttatta ttttgcagct gtacaaaacc attatgattt atttaattat
      241 tggttgatgg gttcgatttc aatcataatt agctatttat tattagtaat tggcggaatg
      301 ttcacgttat taaaattacc taaagtaaca aaacgttggg gtggtaaaac taaaacacat
      361 ttcggtttat tattaactgc gttgagctca tttatatata ttgaaaaaat tatcgaatat
      421 atattggttc ctaatgtcgt agaatctaag tttataatta ttgtgagcat gttggttatc
      481 gctggagcac aatttgtggc atttcaattt attatgcaat acagtagatt ctatattttt
      541 gaattaaaca ctgaagatga tgactaaata attttataaa atactgttgg agtatactta
      601 aataagaaaa tcaacttcaa cgtatatcac catattattt tatactcaat gatgtgaatg
      661 gatttaagtg gattgatatt aaagtaaaag ttatggaata ttgaggtgtg aatatggagt
      721 tcttattatt aattgtcgta gccggactgt attatattat atatttaact gctgtgatgt
      781 attctgaaaa aatagtagta ttgcctataa tcatctatgc cattgtgttt gtaataattg
      841 gtatcactta tatctttata ggcgacagct atgatcaatt aacaaatttc aatgtgattt
      901 tgtatatggg gagtttgttt tatgcatgga tggctattag aaatctttgg aacagaccac
      961 tattactaaa atataagaac attacagata gttcaagtgg aatagttaat aaatctgaat
     1021 acaattcagt tgaaagctta cgtataaata ttgaaatagc taagtataaa gggattattt
     1081 ctttgatagt agctatagta ctaacggtat taatgacatt aaagtcaaca cctcaaatca
     1141 ctgcagaaac acgtgactta agtatctcat ttttcatact cagcttattt atcattatta
     1201 tatttgcagt ttgggatttg tttattagag ttagaaaagg agcgtttgct tttgttgtaa
     1261 taaggccaat attatttagt tgttggttat ttattttgaa tatgatttta tcaagattat
     1321 tataattgta ttacatatag gtgtgggtaa atttttattt atgaaatgat aaaaaagtat
     1381 atactattaa tatctttttt actataatta taaatagtta aataaattga aagacaaaat
     1441 aatgagtagg agttaatgag atggaaaacc aaaaacaagg caatggctta aaaattgcaa
     1501 catgggtatt tattgtatta acagtagtta caccgctatt tggtattgga agtattgttt
     1561 gtagtattaa ttataaaaaa tacgatgctg aaaaaggttc gaagttattg caaattgcaa
     1621 ttatcgtaac aataattgct tttgttttaa atttattagc atatttaggt ttaagataag
     1681 taaaacgaat ttgaagaagc attaagcgac atttgggtgt tgtttaatgc tttttattta
     1741 acggaaataa gtgtagagaa taaattaata gtttaatcaa gagatatttc gaacacatag
     1801 gggcgataac atgactttcg aagaaaaatt aagtgaaatg tatagcgaga ttgcgaataa
     1861 gattagcagc atgataccgg tagagtggga aaaggtatat gcaatggcat atatagatga
     1921 cggaggagga gaagtgttct actattacac agaacctgga agtaatgaat tatactacta
     1981 tactagtgta ttaaataaat atgatatatc ggaatcggaa tttatggatt cagtgtatga
     2041 gttgtataaa caatttcaaa atttaaggaa tttatttaaa gaagaaggac atgaaccatg
     2101 gacatcatgc gaatttga
//
LOCUS       DALAAR010000178         2105 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000178 DALAAR010000000
VERSION     DALAAR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.185"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(147..662)
                     /locus_tag="O2458_002839"
     CDS             complement(147..662)
                     /locus_tag="O2458_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCZ3888658.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            complement(646..1764)
                     /locus_tag="O2458_002840"
     CDS             complement(646..1764)
                     /locus_tag="O2458_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888659.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LYTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN"
     gene            complement(1757..>2105)
                     /locus_tag="O2458_002841"
     CDS             complement(1757..>2105)
                     /locus_tag="O2458_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCZ3888660.1"
                     /translation="NKTSYNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANIS
                     GTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFE
                     EIKERLGTGIIYE"
BASE COUNT          754 a          322 c          225 g          804 t
ORIGIN      
        1 attcacatta caataactaa gtttacattt cttaaattta attacttctt tgcattatag
       61 cgaacgccgt acctaatata taaattaaaa cctttgtttc tttgtacttt taattgaaca
      121 attttatatt gatgagatat ttagtattaa atttttcttc ctataaggtt aatacttgtt
      181 tgatcaattt tatgttgatt tacttctttt aaaaattcat tcaagtagaa cccattcttc
      241 aaatttaaag aatgatctaa cgcataaact gttaattcat attgatgatc ttgatcaggt
      301 ggtgtaggtc ctacatagtg attttctatt tctgaaaaat cttcagccaa caacccacta
      361 gtaaagctat ttttaccttg tacatagtcg ccctttgttc ttgataaatc tgcttttata
      421 gaaattgaat tgccactaac acttacatta gctacactcc aatgaatcca agcaaagcca
      481 catacaggaa ttgcatcata gtcaattaaa gaccaagcaa gatatttagt cccttccggt
      541 aattcaatta cttcaaatgg aaatgactta attggattcc cgttaataga atcactcaca
      601 tctgcacctg ctgcgtattc atatggtata ttgttccctt taaattcagt ttttattttc
      661 atgttgctct ctccttgtaa aataaaatat tattattaaa tatataaaaa ttaataacga
      721 aataaataat attaagtatt gaatagttcc tattgtatat aagtaaccac ccataagtaa
      781 aaatgcgata ttaaaaataa aatataacgt ttctgcataa ttaagcatca ctcttaagtt
      841 actactgcta gaaagtttat ttatagtttg tggacgagtt aaccctgaag aaatacctaa
      901 aaatatcata gaaatagtaa ctgctatgaa actttgatat gtatatatta ttaaaaatat
      961 tactaaacaa atttctgaca caaacttata attcaagtat ttagttaaat aacgtgaaga
     1021 tacaaaaccc ataactgtgt aacaagttaa tactgaaata aattgtaaat tattcagttt
     1081 taaatcatta tatatattaa ttggaataaa tcctataaaa aatcctaata ttaacgctct
     1141 taaaaaagaa taatgcaata tccaaaactt ctcttctggt agaaatttct ttatctttcc
     1201 ctttgtttct cctattaaat ttaattccct attttcaact aatgtgcatc gaataattat
     1261 aattgttaga attgaaaata taccagtcat tattataggc catttaatat ttattccata
     1321 aagataacta cctataatcc ctgaaattaa taatgatagg aacatatagc tatttgactt
     1381 attttgtacg tatttctcat ttgttatatt tcttttaatt attgcggtat caacacccgc
     1441 catcattgag taacttaacc ctaataatat ttgtgccact actaaaatta aatattgatt
     1501 ttgatataat aacaacaata aaccgatgat tttgaatatt tcactaacaa ctaatttatt
     1561 agaatctttt aagttacaaa ttttaaaaca cttctctttg tatagagaga ataaaaatgc
     1621 tgcaatgcca taagacgcca ttaatatttc taattgtatt atggaataac cttgaatcaa
     1681 aaagtaaatt aataaaaacg gtaaataaaa gtatgctcgt gaaaaaattc ggtataaata
     1741 aatcccaagt attaacttat tcataaataa tacctgtacc tagtctttct tttatttctt
     1801 caaactttat atctccttct cttaataatt caattttatt atctgtcact ttaataatcg
     1861 tactagatgt tttatagtta gttttatttt gacttcttag catatatctt acattttcgc
     1921 ccatgtgctt aattgcttca ttttcagtta ttaaaatatc atcggcagtt ccagatatat
     1981 ttgctgaagt aattgctata ggtgaattaa tatatgaaat aaatcttctc atcgttttat
     2041 tttgtacaca tcctatagct atactatctg aattattgag catataatta tagcttgttt
     2101 tattt
//
LOCUS       DALAAR010000179         2032 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000179 DALAAR010000000
VERSION     DALAAR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.186"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(106..435)
                     /locus_tag="O2458_002842"
     CDS             complement(106..435)
                     /locus_tag="O2458_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888661.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(527..718)
                     /locus_tag="O2458_002843"
     CDS             complement(527..718)
                     /locus_tag="O2458_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888662.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            complement(815..1780)
                     /locus_tag="O2458_002844"
     CDS             complement(815..1780)
                     /locus_tag="O2458_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ3888663.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
BASE COUNT          679 a          320 c          215 g          818 t
ORIGIN      
        1 gaggaattaa aattctatta aaaaaataag tcacttatat atcaacttat taagggagtc
       61 ctatactccc ttttttaatt gtaatgtatt ctatcagtat tacctttatt catagtctaa
      121 aatacttact tcaatgtttc taataatatc tcgtgaggcc tcaaagttgt tattaatata
      181 accattttta ttcaatgata aagaaattaa ttggcctgca taataagtat tgtgatttac
      241 tctaaaggcg tgcgtaaatg cttcaccatt ttgtattttc tcaatcaatg ttgctctgtt
      301 cacaaaaatg ggttggctaa tatttatagc tttttcatct tttatattaa attcatataa
      361 tcttaatcca tgtattttaa gatcatcctt atttttctta atgacatatt taatcacatt
      421 atacgttcta gccatttact aatcttcctc cattaagata ttaattcgaa gctctttctt
      481 cttttaaaaa catataacgt tcaattaaaa aatcaaactt tgtagcttat tgcgcatcat
      541 catttgaact ttgtatgcca attttaattt ttaaaccatt aatcatactt tgaaattttt
      601 gctgataact ttctctttct tcttgttgag aatagcaaaa atgttcattt tgtgtaatta
      661 attggtaata tttaaaacaa ttatctaaag ttgttaatcc ataggctaaa taatacaaat
      721 tatctgtttc aagtgctctt tttcaatcta ctaatgaaag atgtatggca cctctactaa
      781 ttgactaata tctgtcatac aacctctccc aaaattaatt tttaaaaata tgttctctat
      841 gccaatctaa atatttcaaa ttaggtttct gacaatctgg tagttcgatt ttttgatttt
      901 cataaatctt taattcttca tataaagcag catctttctc tgcttcttta gataaaacaa
      961 ctctataact gttagaaaaa gtgataaagc ctttcttaaa tgcttttgct aaaaataaag
     1021 acaaacaaat accatttgaa ggatctagtc taataaactc attttcatgc caaggaataa
     1081 tatgtgtaac gactaaaaag tctttagttt taatcccggt aattgcacat ctataactat
     1141 aattttgttt aatccttttt gaaaactcaa aatgagcact tcccttagct tgaacaaatg
     1201 cttcatgatt ccctacgtag taaatatttt tctttattcg agttgccata tcagccattt
     1261 gtttaaaagt tagcgaaata tcattattaa tataattact ttctatttcc actacatttc
     1321 tactcaaact tagtagtttt aaaaaatcat tttttgtaat attactgatt ttatttttaa
     1381 agaaaaactg ctcccactct tctttaattc tttctttgaa ctcccatttt aaattttcaa
     1441 tattatcttt taaaacacga tccacaaaaa gtaagggttt tgaaatgtaa gcggttgcta
     1501 cattattttt tcgctcaatt ttttctattt tcccagctcc aaagaaataa aattgttgag
     1561 caatttgaga taaattagtt ggccgtctaa aaatgaacaa atcgccttct ctgatcttat
     1621 tattctgttt tacatcccaa gtaaactcct tttcagtatc atcactattt gtatagttgg
     1681 tatctaatat aaaatagcga gtgcgttcgt tttctaactc agttttaaag gtgttatata
     1741 gtgactctaa gtatctattt ttatattcgc ttatactcat ctttctatca gctcacttgt
     1801 aaattataaa tatttagcat acttttattt aattattaaa tatcaatttc taaatatatt
     1861 ctgataatta tttattttat atatgtatta ttagccttag agcaatatta ggtgacaaat
     1921 aaaaggctac ctcataaggt aatcctctca agataatgtg acaaaataat tgatgatctt
     1981 taacattaat aagaactaaa ttcataattc ttaaatatat tttctaatta aa
//
LOCUS       DALAAR010000180         1909 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000180 DALAAR010000000
VERSION     DALAAR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.187"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            138..>1909
                     /gene="clfB"
                     /locus_tag="O2458_002845"
     CDS             138..>1909
                     /gene="clfB"
                     /locus_tag="O2458_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCZ3888664.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDS"
BASE COUNT          750 a          318 c          347 g          494 t
ORIGIN      
        1 tagttaacag aaaattttaa gttatataaa taggaagaaa acaaatttta cgtaattttt
       61 ttcgaaaagc aattgatata attcttattt cattatacaa tttagactaa tctagaaatt
      121 gaaatggagt aatatttttg aaaaaaagaa ttgattattt gtcgaataag cagaataagt
      181 attcgattag acgttttaca gtaggtacca catcagtaat agtaggggca actatactat
      241 ttgggatagg caatcatcaa gcacaagctt cagaacaatc gaacgataca acgcaatctt
      301 cgaaaaataa tgcaagtgca gattccgaaa aaaacaatat gatagaaaca cctcaattaa
      361 atacaacggc taatgataca tctgatatta gtgcaaacac aaacagtgcg aatgtagata
      421 gcacaacaaa accaatgtct acacaaacga gcaataccac tacaacagag ccagcttcaa
      481 caaatgaaac acctcaaccg acggcaatta aaaatcaagc aactgctgca aaaatgcaag
      541 atcaaactgt tcctcaagaa gcaaattctc aagtagataa taaaacaacg aatgatgcta
      601 atagcatagc aacaaacagt gagcttaaaa attctcaaac attagattta ccacaatcat
      661 caccacaaac gatttccaat gcgcaaggaa ctagtaaacc aagtgttaga acgagagctg
      721 tacgtagttt agctgttgct gaaccggtag taaatgctgc tgatgctaaa ggtacaaatg
      781 taaatgataa agttacggca agtaatttca agttagaaaa gactacattt gaccctaatc
      841 aaagtggtaa cacatttatg gcggcaaatt ttacagtgac agataaagtg aaatcagggg
      901 attattttac agcgaagtta ccagatagtt taactggtaa tggagacgtg gattattcta
      961 attcaaataa tacgatgcca attgcagaca ttaaaagtac gaatggcgat gttgtagcta
     1021 aagcaacata tgatatcttg actaagacgt atacatttgt ctttacagat tatgtaaata
     1081 ataaagaaaa tattaacgga caattttcat tacctttatt tacagaccga gcaaaggcac
     1141 ctaaatcagg aacatatgat gcgaatatta atattgcgga tgaaatgttt aataataaaa
     1201 ttacttataa ctatagttcg ccaattgcag gaattgataa accaaatggc gcgaacattt
     1261 cttctcaaat tattggtgta gatacagctt caggtcaaaa cacatacaag caaacagtat
     1321 ttgttaaccc taagcaacga gttttaggta atacgtgggt gtatattaaa ggctaccaag
     1381 ataaaatcga agaaagtagc ggtaaagtaa gtgctacaga tacaaaactg agaatttttg
     1441 aagtgaatga tatatctaaa ttatcagata gctactatgc agatccaaat gactctaacc
     1501 ttaaagaagt aacagaccaa tttaaaaata gaatctatta tgagcatcca aatgtagcta
     1561 gtattaaatt tggtgatatt actaaaacat atgtagtatt agtagaaggg cattacgaca
     1621 atacaggtaa gaacttaaaa actcaggtta ttcaagaaaa tgttgatcct gtaacaaata
     1681 gagactacag tattttcggt tggaataatg agaatgttgt acgttatggt ggtggaagtg
     1741 ctgatggtga ttcagcagta aatccgaaag acccaactcc agggccgccg gttgacccag
     1801 aaccaagtcc agacccagaa ccggaaccaa gcccagaccc ggatccggat tcggattcag
     1861 acagtgactc aggctcagac agcgactcag gttcagatag cgactcaga
//
LOCUS       DALAAR010000181         1866 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000181 DALAAR010000000
VERSION     DALAAR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.188"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..412)
                     /locus_tag="O2458_002846"
     CDS             complement(<1..412)
                     /locus_tag="O2458_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ3888665.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLIN"
     gene            complement(405..572)
                     /locus_tag="O2458_002847"
     CDS             complement(405..572)
                     /locus_tag="O2458_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888666.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(547..1398)
                     /locus_tag="O2458_002848"
     CDS             complement(547..1398)
                     /locus_tag="O2458_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ3888667.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          680 a          295 c          210 g          681 t
ORIGIN      
        1 aattaattaa tttttctaga tattgcggtg aatttttaac taaactatct acttgcgaac
       61 caataatagg tatcaataaa ttaacaatta acgtaataac acctactaca gctagataaa
      121 taatagaaat acctgcaact cttggtattc tataacgttc catcatattt acgattgggt
      181 taaatagata gaatagaatt aaagagacga tgataggtgc tgctatcgtc ttaaaaacga
      241 tgataaaagg atcaaaaaca tatgatactt tttcgaaaat aaaaataaca atacccaaca
      301 atactagcgc tattaatgag aagactaaat cattcccacc aacaaacttc atataccgtg
      361 attcggtaaa ttttaaaaaa ttctttctta tattcttttc attttcattc aattatagat
      421 tcaccaccac ttaaaatatt cataagatgt tgtataaaaa cagtccactt atgtaaaatt
      481 actaacatca atgataccat acaaataccc tttacagtaa cgaaacgccc gtgtaataca
      541 cttaatttag gattgattaa tcctttcttc aaaatgatga atgtgtaatg ttaaatatat
      601 aatttcagac tcactgatat taacatcaaa ttgtttttgt atcatagtta aaattttata
      661 tgctgtgtta tagcaaatcg gatagtgatt tttaatcata gacacaaaat catcttgtgc
      721 atgtatatat tcttttcttc ttaatcggcg aattaaaaat tgtacgtgcc ttataaaacg
      781 ttggtattgt aatgattcct tatcaacagt tgtcacaagg tctgattcaa tgatatctat
      841 acctttttta ataacattat tgatcaaggt catctcatgc atagataaat cttctgtatt
      901 agatgcaata tgtaatgcaa taaatcctat ctcatcttca ggaaaatgta catctaatgc
      961 tgcatttaac tgatcaatca cctgtttagc aatatgatat gcatcactat ataactgcat
     1021 agtttccata acaaatggat tgctaataac ttgattttgt tttaagcgtt tataagcaaa
     1081 tataatatga tccgttaatg aaactacaag ttgttttgaa tcaacattca tcgcagtatt
     1141 agaaataaaa ttcaacgaat caataattac ttgtaataca ttatcatcag cgatttcaac
     1201 taaactttta taatgtgctt tttgttgctc actttctaat ttataaattt tctctattgt
     1261 aatagtttgg tcgtttaacg ccattccctc ttttttgtta aaaccaatac ctttaccgat
     1321 taaaataact tcttgatcat tattagtaca tactacgaca ttgttgttca atgttttagt
     1381 aacaatatat tctcccatta tcatcaccta tttttctatt attaagatta tatatcggaa
     1441 atgtctaact tgaaagtata ataatttaaa tacttgtgtt ctattacacg cctatcctat
     1501 atgatatatc ttaatttaat ttcgatgtct ctcaaagtgg aataactata ataaaaaaat
     1561 ctgactccca tatttacaaa taattctatt tatttatata ttatctgaat taatactcaa
     1621 tacaaactaa aacgtactat taaattgtgc aaagctaaaa caaatttata ttcatctatc
     1681 caacaatatg tcttatcaat ggtatagtct ttgcacacca atggaggaaa taaatctcaa
     1741 ccttactata ttaatatata atcaaatctt agattaacta gtgtaatgat acagatgata
     1801 attgagtaca aatttaaaac cctgagattt tcgctttaat ttgaaaacct cagggtttat
     1861 ttgatt
//
LOCUS       DALAAR010000182         1773 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000182 DALAAR010000000
VERSION     DALAAR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.189"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..1318
                     /locus_tag="O2458_002849"
     rRNA            <1..1318
                     /locus_tag="O2458_002849"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1391..1505
                     /gene="rrf"
                     /locus_tag="O2458_002850"
     rRNA            1391..1505
                     /gene="rrf"
                     /locus_tag="O2458_002850"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1516..1590
                     /locus_tag="O2458_002851"
     tRNA            1516..1590
                     /locus_tag="O2458_002851"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1548..1550,aa:Asn,seq:gtt)
     gene            1592..1663
                     /locus_tag="O2458_002852"
     tRNA            1592..1663
                     /locus_tag="O2458_002852"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1625..1627,aa:Glu,seq:ttc)
     gene            1671..1746
                     /locus_tag="O2458_002853"
     tRNA            1671..1746
                     /locus_tag="O2458_002853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Val,seq:tac)
BASE COUNT          464 a          395 c          502 g          412 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttactatct agttttgaat gtataaatta
     1381 cattcatatg tctggtgact atagcaagga ggtcacacct gttcccatgc cgaacacaga
     1441 agttaagctc cttagcgtcg atggtagtcg aacttacgtt ccgctagagt agaacgttgc
     1501 caggcataat attaatccac agtagctcag tggtagagct atcggctgtt aaccgatcgg
     1561 tcgtaggttc gagtcctacc tgtggagcca tggctccttg gtcaagcggt taagacaccg
     1621 ccctttcacg gcggtaacac gggttcgagt cccgtaggag tcattatttt ggagaattag
     1681 ctcagctggg agagcatctg ccttacaagc agagggtcgg cggttcgaac ccgtcattct
     1741 ccaccatttt gattattaaa ttatatgaat aag
//
LOCUS       DALAAR010000183         1638 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000183 DALAAR010000000
VERSION     DALAAR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.190"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            49..603
                     /locus_tag="O2458_002854"
     CDS             49..603
                     /locus_tag="O2458_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ3888668.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            complement(809..>1638)
                     /locus_tag="O2458_002855"
     CDS             complement(809..>1638)
                     /locus_tag="O2458_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ3888669.1"
                     /translation="LDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFE
                     THIKSRISYYFSEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKH
                     YINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPS
                     DVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVF
                     DLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
BASE COUNT          617 a          234 c          224 g          563 t
ORIGIN      
        1 taaaattact ttgtaatcga ttgcaaataa attataggag aaaataaaat gaataaaaaa
       61 ctattaacaa aaacattgat agcaagtgct ttagttttaa caacagtagg ttcaggtttt
      121 cattcttctt caaattataa tggtattaat aacgttgcaa aagctgagca aacgacagat
      181 aacgaattgt ggaaaaatgt aagagacgct ttaaaagaag cgaatattat cgataaaaca
      241 gataatgaaa atgtcaaagt gacttataaa ttagcaaatg gcggtgaaaa cagtattgaa
      301 ggtaccgcca atttaaatga acttagcaat tctaataaca aaccgatcaa tagcgatagt
      361 gtaaaaagca ttgatttgtc aaggaaaaat cccaatggag atagatttga cgcaaatgat
      421 gcatggaaaa aattaactga taaattaaaa gaaaagcaga ttgtgaaaga cggtgataca
      481 gtaactattc atagtaaaga cgaaaaagac cctaaaatct cagctaaagt cggtgaagtc
      541 tataatgata acaaacgatt gatgttgttt aaaaaggata tagataaaat cactataaac
      601 tagttataga taaagacagg ttactttttt aatgtaactt gtcttttttt aagaggcagt
      661 atctcaaata ttaatttata atttctaaat ctatatctat actttatcta tttctatata
      721 cacactcatc cctcactgca acacagggcg tttctcagcg tcttaaaata aaaaaacgcc
      781 actcgtaagt gacattaaaa aatatctttt ataacatatc cagtattttt gtttgatctg
      841 gaaaattcat ttttgtatat aggtcatcta tagtaattgt atagaaagaa tgtgaataat
      901 tacttattag tttatccata tttttcatca attttatgta atcatgttta ttcaaaaata
      961 gacatagtga aatcaacaaa tcgaatacat aactaccatt cattgtagga acgtattctt
     1021 tatcgtatat tttgttataa ttggcgaata tattagctct acttttagcc ctgtctattt
     1081 tataatcata caatctttct tcatgcgcac acacgttacg aaacatatgt gcttgttgta
     1141 gtatactgtc tacatctgat ggagttattt gaacacgtgt tttataatct ctttccaatt
     1201 tccttttata gtctttagca acttccaatc gaagatcatc atccaaattg gaatacattt
     1261 ttgaaacatt acctaaagtt aaataattca ccaatatcca tagtggcact ccattatgag
     1321 tattaatata atgttttaat ggtttatttt ttctattact cataaccgag ctaaatgtag
     1381 caaccatttt cacgatgcta tctgtcttac ttgtgtcaga tgaataattt ttaaagtata
     1441 aatatgaatg tggttctcta tatttttcgc taaaataata tgaaattctt gatttaatat
     1501 gagtttcaaa tactaacaaa tactctaata aaacattcct aaattttcta tctagtttgt
     1561 ataaagaaaa gacttcttta aaatgagtac cttgcttata tttatcagga accaaaaaat
     1621 taccattaac atctagtt
//
LOCUS       DALAAR010000184         1625 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000184 DALAAR010000000
VERSION     DALAAR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.191"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            340..741
                     /locus_tag="O2458_002856"
     CDS             340..741
                     /locus_tag="O2458_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCZ3888670.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            701..793
                     /locus_tag="O2458_002857"
     CDS             701..793
                     /locus_tag="O2458_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888671.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1110..1439)
                     /gene="ecb"
                     /locus_tag="O2458_002858"
     CDS             complement(1110..1439)
                     /gene="ecb"
                     /locus_tag="O2458_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCZ3888672.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          585 a          241 c          210 g          589 t
ORIGIN      
        1 caaaacagct atatgtataa caatttggag tgtttgtcta tccaacttag caaacaaaat
       61 gtagagaaat gtaaatatcg ttttagcaat aaaaattatt caatcatagt tgagtgacgt
      121 ttactactat tattatacta tcaacaaatt caataatacc tcaaataaaa ataataccat
      181 caaattcatc attattcttt ttcgattgca ttaaacctta ttaatttgca cttaatttta
      241 attaatgaag tgttaactgg taaatgtatg aaaccgtttg taaaatagca gtgtactcgc
      301 attagtaagt acaaatttat tttacaggga gaacttatta tgaaaaaaaa tatcacaaaa
      361 actattatcg catctacagt aatcgctgca ggtcttttaa ctcaaactaa tgatgctaaa
      421 gctttcttta gttatgaatg gaaaggttta gaaatcgcaa aaaatttagc agatcaagct
      481 aaaaaggacg atgaacgtat tgataagtta atgaaagagt ctgataaaaa tctaactcct
      541 tacaaagctg aaactgttaa tgatctgtac cttattgtta aaaaattaag ccaaggtgat
      601 gtgaagaaag cagttgtcag aattaaagat ggtggtccta gagattacta tacttttgac
      661 ttaactcgtc ctttagaaga gaacagaaaa aatattaaag ttgttaaaaa cggtgaaatc
      721 gactcgatta cttggtatta aaaaacatac tgaattaaat agttgtacgc caaacgttag
      781 aaaacaatgc taacgtattg gcgtgctttt ttattttaag taacttccaa tttatttagc
      841 atctttacga ctgtttaata aagcacgtat gattaacacg gttgccacta tatccgttac
      901 aatttttatg attgttaata cattcgattt ccttttcaca atagccacct cgcttgttca
      961 aaacataaac aactattgca ttcactttta agtaattatt tatattattt atcccaataa
     1021 gctcaccatt caaataaaca caatattata aaataattat acctttggat atagcaaaaa
     1081 gccacactct atagcatggc ttctatcaat tattttaaag tattatattt taaaactaga
     1141 tcgatttgtc tttgtaattt tttcttttca taactgtgtt ggaaatgaat taaattaaca
     1201 gctctttgtg ctttacggtg tgttgcaaca gttcttgtac gtttgaaaaa gtttacagct
     1261 ttttgtgcat ccacaacttt tttatttact tgttttttaa agtttgtttg atactgatca
     1321 tattttttag cagcttcaac gtttttagtt tgtgcatgag attcaccggc aaacgttgat
     1381 accgttaaac taatagcagc tatgcttaaa attgatttcc caataaaatt ctttttcata
     1441 attaataaca cctatcctca aatatttatt ttgtatatat ttataataat agacagatac
     1501 aaaaaactac attaactatt tattaactaa acattaatat ttgtgtcact acatttctga
     1561 atttcaagaa ttttgctact ccatatttta accatcaaat tattgatata atttatgact
     1621 ttaga
//
LOCUS       DALAAR010000185         1612 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000185 DALAAR010000000
VERSION     DALAAR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.192"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(328..528)
                     /locus_tag="O2458_002859"
     CDS             complement(328..528)
                     /locus_tag="O2458_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ3888673.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(885..1571)
                     /locus_tag="O2458_002860"
     CDS             complement(885..1571)
                     /locus_tag="O2458_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ3888674.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          496 a          298 c          201 g          617 t
ORIGIN      
        1 attatgcgat gtggtatcct attattgtta aacattttga gtgtttaaca ttacttcttg
       61 ttatcgccat atacagactt taattctcgt cattattaaa gttctgtatt ggcgcttttt
      121 tatattcaaa atcaaaagtc ggacagatga aaagtaaaaa acttttccat cagttcgatt
      181 tattttagaa ccattatata ttgttaaatc tccaactaaa tttattggac aaaaaaataa
      241 cggctagtgc ttttaccaca ccaactgtta tcgatttaaa gcataaaaaa acagccctat
      301 agtaaggact gtttgaataa gttaaaatta cattttaact acgtttgcag cttgctcgcc
      361 acggtcgcct tcaacgatgt cgaattcaac tttttggcct tcttctaatg atttgtatcc
      421 atcttcagcg attgctgaga agtgtacgaa tacgtcgcta ccatcttctc tttcgatgaa
      481 accaaaacct ttttctgcat taaaccattt aactgtaccg ttattcatat tgaatacctc
      541 cgtcgtgctt ttgcactaaa tattttgtaa caaattcata aatgaaaagg agaatattct
      601 atataaataa ctcacatttt tcttcacgct ctttattact taactttcat tatacacttt
      661 taaatataga agtaaagcat tattttattt tctgtgataa tacacattga actatatagt
      721 aacattccat tttaatacta aatttaaaat ttttattttc taatattaaa tacacttact
      781 tttgatacta tttacttttt taattctgaa ttgataataa atcatatcgt gatctaaaaa
      841 ttataaaagt gccactagca gcagtgacac ttttacaatg agcattattg acctgaatca
      901 gcgttgtctt cgctccaaat atttaatttc tctttttttg cttgtgcttc actttttctt
      961 aaaagttgtt catgtgtatt gttaggttta taaacataag caactttagc caagccttga
     1021 cgaactaaag cttcgtttac catttttcca tcagcataaa tatacgctaa gccacgtcca
     1081 tatttatcag ttctttgacc tttgtcaaac tcgacttcaa ttttctttgc attttctacc
     1141 atttttttcg taaatgcact tgcttcagga ccatatttct ctacaccttt tttaggatgc
     1201 tttgtttcag gtgtatcaac caataatagt ctgaatgtca ttggttgacc tttgtacatt
     1261 aatttaaccg tatcaccatc aatcgcttta attaatgtcg caggttcttt atgtaatttt
     1321 ttagttgaag ttgcactata tactgttgga tcttcagaac cacttctatt tacgccatta
     1381 tctgtttgtg atgcatttgc tgagctactt agacttgaaa ctacaactaa agttaacact
     1441 aaacaactag tagcgaaata gaaaaacctc tttgcgtatt gccctttcga aacattactg
     1501 atagccatcc ctataagtaa tattgaaacg attgccatac atatgccagc acttaataag
     1561 tattctgtca taactaacac ctctttcttt ttagttaatt ttaatattaa ac
//
LOCUS       DALAAR010000186         1547 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000186 DALAAR010000000
VERSION     DALAAR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.194"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            71..373
                     /locus_tag="O2458_002861"
     CDS             71..373
                     /locus_tag="O2458_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888675.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            629..994
                     /gene="crcB"
                     /locus_tag="O2458_002862"
     CDS             629..994
                     /gene="crcB"
                     /locus_tag="O2458_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCZ3888676.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            991..1344
                     /locus_tag="O2458_002863"
     CDS             991..1344
                     /locus_tag="O2458_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCZ3888677.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          489 a          241 c          221 g          596 t
ORIGIN      
        1 ttaataaaac tattgtttta tgattgttta tgatacgata tgtaaatgat aatcatccag
       61 gaggtctagc atgtctcgtt caaaaaaata cttttactta tctagcttaa tgattatttt
      121 aagctttttc tttaatacaa ataacgtttt cctaagtgga ctttttaatt cttttattaa
      181 attaatactt ttctgcagtg ttattaactc aattgtacta attttgtcta taatttttgc
      241 agatcgttca attaaatcac taaagcctga tgcagattgg attagaattg cgagtaaaag
      301 tttgccttgg attattctaa ttgttatttt agtacatatc ttttcaattg ttcgtacatt
      361 cggttttatt taaaaaagtt aatatgtcat tgtagcctta atacaaaaca atacaatgta
      421 tcatgctata atgagtaaaa caatttgata acgttgttgc gtataaaaaa tattagattt
      481 tcgaaatcat aactatgcat ctaatcgcta tagttataca acaagatata acatataatg
      541 aggtttgata atgcatcgac aatttttgtc gtcgcgttgc caaaacctct tttttaaatt
      601 caaactactt cttttcgagg tgaaccaaat gcaatatgta tatattttta tcggtggtgc
      661 tttaggcgct ttattacgtt acctcatttc ttttctgaat actgacggag gttttccaat
      721 cggaacactg atagccaatt tgactggtgc ctttgtaatg ggattgctaa cagccttaac
      781 aattgcattt ttttcaaacc atccgaccct aaaaaaagct attacgactg gttttcttgg
      841 tgctttaacg actttttcaa catttcaatt agaattaata catatgtttg atcatcaaca
      901 atttataact ttactactat atgctgtaac aagttatgtc tttggtattt tgttatgtta
      961 cgtcggtata aaactaggtg gtgatttatc atgatatcaa tcattttagt catgattggc
     1021 ggcggttttg gcgcaattgc tagaagtgcc attactgatt attttaatca taaatttact
     1081 tcaaagttac ctatcgcaac attgatagta aatctagttg gtagtttttt aattggatta
     1141 actataggct tatcaatttc aatctcatgg ttccctgcgt tctttgttac cggtttttta
     1201 ggtggcttaa caactttctc aacgttagcc aaagaactta cactaatgat gacgccaaaa
     1261 tttaatatta acctttttct caattattca cttttacaat tcatcattgg atttatagct
     1321 tgttatattg gctatcatat ttaaaaataa aatgcttcat tcagcaaata ggtaaattac
     1381 gacaccttcc tgaacgaagc attttttaat tttcatgcaa atttttaagc accatataat
     1441 gcctaccaaa tttcaataat ctttgttgcc gtttaaataa tgtgaatgtc aataaattct
     1501 ccaaactagt cgaaaataaa gggagtggga cataaatccc taaaaaa
//
LOCUS       DALAAR010000187         1528 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000187 DALAAR010000000
VERSION     DALAAR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.195"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..130
                     /locus_tag="O2458_002864"
     CDS             <1..130
                     /locus_tag="O2458_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888678.1"
                     /translation="SQEINNRESEKGCLWGISMLILLFLLILFGITACSSSIHFIN"
     gene            441..701
                     /locus_tag="O2458_002865"
     CDS             441..701
                     /locus_tag="O2458_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888679.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(754..1104)
                     /gene="scn"
                     /locus_tag="O2458_002866"
     CDS             complement(754..1104)
                     /gene="scn"
                     /locus_tag="O2458_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCZ3888680.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          505 a          208 c          200 g          615 t
ORIGIN      
        1 aagtcaagaa attaataata gagaaagtga aaagggttgt ttatggggca ttagcatgtt
       61 gattctactt ttcttattga ttctgtttgg aataactgct tgttcatcaa gcattcactt
      121 tattaattag atttttttac ttggaggtat catgtgaaga accatacaaa tataattaat
      181 atcttattag ttatagtcaa ctcattaact cattttctaa ctctaaacac ctcatttttt
      241 aataattcag catcggattt ctgttttatc ataggggcta tatttttctt aatcggaatt
      301 tttgttgcaa tatacggtat gaagcgagca acatattggt taaacttatt gattttattt
      361 accaatattt tttattttct acacttctgt gtgttacttt tgttaaaata tataggattt
      421 aaattattta tttatgaagg gtgtgtattg ttatttatct aatttatagt ctaattgtct
      481 atttcatctg cattataaat tttttcataa tggctgcacc attcttaatg actaatgctg
      541 attatgtatg gacgcctatg actacagtta cgttatttat tttgagtttg attattttct
      601 tgatatttat aaaaacaaaa gatgtcgttc atttaacaat tttcatatta aacttacttt
      661 tttcaatact ttattgtttg cctatactgt tttatttatg aacactttta tattatctag
      721 aaaattaaaa ccacccgtaa aagggtggtt tttttaatat ttacttttta gtgcttcgtc
      781 aatttcgtta taaatctttt gaagttgata ttttgcttct gacattttct taaagtcttt
      841 tgacttaaga gcatacattg ctttttgacc tgaaattttt atagttcgct tataataagt
      901 gtttaaactt ccagtagcta attcattaac atttagttca tctaataacg attttaattc
      961 attagctaac ttttcgtttt gatattcatt cgatgttggc aagcttgtgc tagcttgtgc
     1021 ctcattttta tctagattag ttactagtgg tgatgctaaa acgattgcta aagttcccgc
     1081 aagtatagat tttctaattt tcatttttta ttctccttag taattagtac taatttattt
     1141 taatgactga ctaaaaaaaa gtacattaat tattcattaa ctatatagat cttcttatta
     1201 aatgaaaatt aataaattat caaattgaca taccttattg tatttataga aaaacaaaaa
     1261 aaggtaagca ccgaaatgct tacctacttc ccataaacaa tataacacat atacattgat
     1321 ttggaaagcg caaaaataaa tttaatatac tagcccgaag gggagcaata cataaaaaat
     1381 gaaaggcgct ccttgaaaac gcccaaggta atattaacat aaaatggcta ctattgcatt
     1441 atctaatttt atttaattaa aacaaatata tatagcacaa aaaaactagc ccgaaggcta
     1501 gcttatgaat agatgaaaat ttgaacac
//
LOCUS       DALAAR010000188         1495 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000188 DALAAR010000000
VERSION     DALAAR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.196"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(335..1426)
                     /locus_tag="O2458_002867"
     CDS             complement(335..1426)
                     /locus_tag="O2458_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCZ3888681.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          474 a          263 c          207 g          551 t
ORIGIN      
        1 agaaagcctt ttcaaatatc aattgcattt gttgaaaaat gttcacattt cagcaacaaa
       61 tgtgtgaatc tattatattt tacagataat tcatagttat aattttaatt actgctatca
      121 gtcataatct ttaataattg tgaaattgaa caagtgtcga agtattataa caatcgttaa
      181 gtgcagtata cctttatttc tatacaaaaa ataatagcca ctctaaaagt aatatactct
      241 tagaatggct gtaagtttaa aattgtcgat tcaaagataa tattctactt caacatgaca
      301 tattagttaa gtagtctata gtttccttgg aatattattt taacttttta gcgttatata
      361 aatcagctaa aggctttaat tcatcataaa atgcttggaa ttcatctaaa tccatttgtt
      421 gacccgcatc actaagtgca acagatggat ctggatgcac ctcagccata actccatcag
      481 caccaactgc taatgctgct ttcgcagttg gtaacatgat atctttacga cctgtactat
      541 gcgtaacatc taccatgact ggtaagtgtg taccttgttt taaaattggt actgctgaaa
      601 tatctaaagt gttacgtgtc gccttttcat aagttcggat tccacgttca cataaaataa
      661 tgttttgatt accttgtgaa gcaatgtatt cagctgcata aacaaactct tcgattgtag
      721 cagataaacc acgttttaat agaataggct ttttcgtacg gccagcttct tttaataact
      781 cgaagttttg catattacgt gcaccaattt ggaatacgtc taaatactca tcagccactt
      841 caaaatcatt tggatttacg atttcgctga caacatttaa atcatattta tctttaatct
      901 gtttaagtat tttaagtcct tcaacaccta ggccttggaa atcatatggt gatgtacgtg
      961 gtttaaatgc accgccacga ataaattttt cacctttagc atgtaagttt ttagcaacag
     1021 cttcaacttg ttcaaatgat tcaactgaac atggcccaaa tacaaatgat ttattgccgt
     1081 ctccaataat gcccccatta tcaaatgtta caatcgtatc ttcaggtttc aacttacgtg
     1141 atacatataa atgtttttca ttttcagatt tttgtaaatc tgtagaggct ttgaaaattt
     1201 ctttaaataa ttgcttaata gtattatcgt tgaatggtcc tttgttacta tcgattaagt
     1261 cgttaagcat ttctttttca cgttgtggat cataaatacg tgtaccttgt tttaattttt
     1321 cttccccaat tttttgtgct agttcaccac gtttagataa taagtctaaa atttgatgat
     1381 tcagtgatac aatctcactt ctgtatgatt ctaatttatt actcatcctt actcacctct
     1441 actaattatt tcaagtatat tccactttaa agcaataaaa tataatgtta aaaaa
//
LOCUS       DALAAR010000189         1458 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000189 DALAAR010000000
VERSION     DALAAR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.197"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            478..579
                     /locus_tag="O2458_002868"
     CDS             478..579
                     /locus_tag="O2458_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888682.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            589..861
                     /locus_tag="O2458_002869"
     CDS             589..861
                     /locus_tag="O2458_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCZ3888683.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            905..>1458
                     /locus_tag="O2458_002870"
     CDS             905..>1458
                     /locus_tag="O2458_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCZ3888684.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKN"
BASE COUNT          557 a          187 c          221 g          493 t
ORIGIN      
        1 tttttaacgg gctcattagg cgacatgttc ctgcatgtcg tctttttaca tttttatagt
       61 aacatatctt ttatttaatt gtctattttt attagatgtg taacaaaatt ttgaagtatt
      121 ttccgtatat tgcctgctaa acatgtcaga tttgaaagga aaatcatact gtggtagaaa
      181 tcaattatga ttatatggta gtacttataa ataagtgatt tctattgaat atgtaatgaa
      241 gttcatatat taatataact ttcattgata tagaagtcgc aatgattaat ttaaacaata
      301 aaaaacaacc atcatttcca aactttgaca acgaatgatg gttaaagaaa atctcattta
      361 gtcaccgtct ttttaacggg ctcaataggc gacatgttcc tgcatatcgt ctatttatac
      421 actgcacaat ttaataataa tacaattata ctttgatgtc catttaacat aaagtggttg
      481 aaatttgcag caaataaatt aaccttgact attttcagaa aattctgtat aaataacgaa
      541 aaagagatat ggattaaata tgaagaggtg tttttataga agaataaaat ttttggcact
      601 atattatatt taactttagc acttggatta tcaacagcag cttatgcatc tacagaatac
      661 gcagaaggag gcacttggag tcatggtgtc ggcagtaagt atgtttggtc gtattattat
      721 catggtcata aaggacatgg tgcaacagct attggaaaat atagatcatt tagtggttat
      781 acaagagctg gtgtaaaagc aaaagcatca gctactaaac ataattggtg ggtcaataga
      841 gcgtattata acatttatta aatactaaaa aatcatagaa aataaacaac gcaagaggta
      901 caacatgaaa tggttcaaat taatacttga tgttacaatc ttcattctga ttgctatatt
      961 gttatttgtt tatacatata aagaaaacga agaaatattg cctgatacta aataccctat
     1021 agcggtaact gactggaata aaaaatatag taaaaatgag atttataaac gtatagatca
     1081 attcgctaaa aatgagaacg tagcaatcta taaatcaact tcaaattata caaacaaaaa
     1141 cgtagataaa gatatatatg tatttaataa atcaaaagca gcaactatca ctccttttaa
     1201 tgctaaatat aacattcatt atttaagtga cgacgaagta ttaaaaaaag atatcaaagg
     1261 gagttatttt gtaaaagaca aaaattttga cgtgtctaaa ttcataaatt ttttaaaaga
     1321 atatggtgtt actgctgaat catacaaaat agatcatatg atgattgccg ttggtgtcat
     1381 taaacaaatg aatatagtag ttccattatc ctcactttta atcgtttatt ttatttatta
     1441 tattttcgaa aagaacat
//
LOCUS       DALAAR010000190         1429 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000190 DALAAR010000000
VERSION     DALAAR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.198"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..380)
                     /locus_tag="O2458_002871"
     CDS             complement(<1..380)
                     /locus_tag="O2458_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCZ3888685.1"
                     /translation="MKRASYSVETKYKAVEMKAAGFPTKEIMKELNIRNRTQVKNWWR
                     WYRNGESYRFSQHVGKQYTYGYRKITALINQCYTSPINHKRVQRVMQKHHLNCRVRPK
                     KTTRIGKPYYKTDNLLQRQFKASCP"
     gene            complement(893..1390)
                     /locus_tag="O2458_002872"
     CDS             complement(893..1390)
                     /locus_tag="O2458_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ3888686.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
BASE COUNT          476 a          233 c          174 g          546 t
ORIGIN      
        1 ggacaactcg ctttaaattg tctttgtaat aaattgtccg ttttataata cggtttacct
       61 attcttgtcg tctttttagg tctaactcgg cagttcaaat gatgcttctg catcactctc
      121 tgtactctct tatgattaat tggtgatgta taacattgat taatcaatgc tgtaatctta
      181 cgataaccgt aggtatattg tttaccaaca tgttgtgaaa atctataact ttccccattt
      241 cgataccatc gccaccaatt tttcacttgt gttctatttc taatatttaa ttctttcata
      301 atttcttttg ttggaaatcc tgctgctttc atttcaactg ctttatactt tgtttctact
      361 gaataagaag ctcttttcat agaaaaaaaa cacctccgta tgattcattt taatatgaat
      421 tcaacgaaag tgtttttata taattcccac tattggggtc agtctaaaat taaaattgat
      481 aactttttta ataaaataga tacactaatt ttaaaacaag gtaaaaaaat agaattatta
      541 aaacaacgca aacaaggctt actacaaaaa atgtttgtgt aattctgata acgtcctatt
      601 atgttaattg aaaatagaca aaaataaatt gagagcatcc cttaccgcaa agtgaaggat
      661 gctctatttt tgttgaaatt aatataagct tcattctgat agatagtcat taataattgt
      721 ttgcaaatcg ttattaagta ccgttgttac agcgtcatca ttcataaaat cctcgtagat
      781 tttatcaaga atttattcct cttacagaca ttcgcgagaa gtccgtttta attttattag
      841 aagtaattca ggtttgaacc tacctaaatg aatatatgag ttatctttta tactacaaaa
      901 tatattcaga tttcaataat gacataaaat aggcatcttt atatttacct ttagtgtaga
      961 attgctcttt aagtaatcct tctgttttaa atccttgtga ctcgtatata tgaatagctt
     1021 ttttgttatc tgcatcaaca tatagataga tcttgtgcat atttaaaata ttgaatgcat
     1081 aaattatcgc tttttcgaat gcgaattttg cataaccttt accactgaac tcaggtttaa
     1141 taattatttg tatttcacaa ttacgatgga tgtaattaat ttctactaat tcaacaatac
     1201 ctacgacttg attttcatct tcaacaataa aacgtctttc cgattcatct aataaatgct
     1261 tatcaaataa atgttgaagt tccgttaagg attcataagg ctcttcaaac caataagaca
     1321 taatagaata ttcattattt aattcatgaa caaaaagtaa atcactatac tctaatgctc
     1381 ttagtttcat aatcccactc ccaaaatttt cttatatatt tgcattata
//
LOCUS       DALAAR010000191         1361 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000191 DALAAR010000000
VERSION     DALAAR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.199"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(142..1098)
                     /locus_tag="O2458_002873"
     CDS             complement(142..1098)
                     /locus_tag="O2458_002873"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ3888687.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
BASE COUNT          431 a          223 c          201 g          506 t
ORIGIN      
        1 acaattttta tcataaagtg tgatagaaat aatgattttg cataatttat gaaaacgttt
       61 aacacaaaaa agtacttttt tgcacttgaa aatactatga tgtcattttg atgtctatat
      121 ggttagctaa ctatgcaatg actacactgc tattggagct tttattgctg gatgtgattc
      181 atagtcaaca atttccaaat cttcataatt tatgtcgaaa atagacttgt cactgttaat
      241 ttttaatgtt ggaggattga agctttcacg tgctaattgt gtttgaatcg catcaatatg
      301 atttgaataa atatgtgcat ctccaaatgt atgcacaaat tcacccactt caagtccaca
      361 ttctttggca ataaggtgtg tcaataaagc gtagcttgcg atattaaatg gcacacctaa
      421 aaagatatct gcgctacgtt ggtataactg gcaacttaac ttaccatctt ggacataaaa
      481 ctggaacatg gtatgacaag gcggaagtgc cattgtatca atttctgttg gattccatgc
      541 agatacgatg tgtcgccttg aatctggatt atgcttaatt tgttcaatta ctgttttaag
      601 ttgatcaaaa tgattaccat ctttatcaac ccaatctcgc cattgtttac cataaacatt
      661 tcctaaatcc ccgaattgct tcgcaaatgt atcatcttca agaatacgtt gcttaaattg
      721 tttcatttgt tctttatatt gttcgttaaa ttcaggatca ctcaatgcac gatgcccgaa
      781 atctgtcata tctggacctt tatactcgtc tgatttgata taattttcaa aagcccattc
      841 gttccatata ttattattat attttaataa gtattggatg tttgtatctc ctttaatgaa
      901 ccataataat tcggttgcta ctaatttaaa agaaactttc tttgtcgtta atagtggaaa
      961 tcctttagat aagtcaaagc gaagttgatg accaaatttc gaaatcgtac ctgtatttgt
     1021 gcgatcattt cgtgtatttc ctatttctaa aacttcttca caaagactgt gatatgctgc
     1081 atcaaatgaa ttcaacatat gcgataacac ctcattttca ttatttatag tatgtatatt
     1141 tagtttgata taacttaact ttatgtagca ttttgttatc actcatttta ggaatatgat
     1201 attaatatca tgaattccgt tactttattt ataaaatgct gattaagtac ctaccccatc
     1261 gtaacgtgat atatgtttcc aattggtaat tgtttaccca aatctataac tttaatgcta
     1321 aaaaatttta aaaaagaggt taacacatga tttgaatatt a
//
LOCUS       DALAAR010000192         1344 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000192 DALAAR010000000
VERSION     DALAAR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.200"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            300..1259
                     /gene="hyl"
                     /locus_tag="O2458_002874"
     CDS             300..1259
                     /gene="hyl"
                     /locus_tag="O2458_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCZ3888688.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          513 a          200 c          214 g          417 t
ORIGIN      
        1 ttagttttaa agaaattaat caataaaatt agctatgtct tttccttgtt tcataaagaa
       61 tttattcaac tttgactaac cctcgaaatt gaaatgcttc ctttcaaatt ttaaataaaa
      121 gttaaaaaca tattcttaaa ataatcactc atcatcactc agtaatttat cagttgctac
      181 attaaatata ctaatataaa cgattataaa tatttgatat gtctcaactg caatattcta
      241 aattgacata ttgattattg tttcctcaaa tcgttttaaa aaatagaagg atgatgaaaa
      301 tgaaaacacg tatagtcagc tcagtaacaa caacactatt gctaggttcc atattaatga
      361 atcctgtcgc taatgccgca gattctgata ttaatattaa aaccggtact acagatattg
      421 gaagcaatac tacagtaaaa acaggtgatt tagtcactta tgataaagaa aatggcatgc
      481 acaaaaaagt attttatagt tttatcgatg ataaaaatca taataaaaaa ctgctagtta
      541 ttagaacgaa aggtaccatt gctggtcaat atagagttta tagcgaagaa ggtgctaaca
      601 aaagtggttt agcctggcct tcagccttta aggtacagtt gcaactacct gataatgaag
      661 tagctcaaat atctgattac tatccaagaa attcgattga tacaaaagag tatatgagta
      721 ctttaactta tggattcaac ggtaatgtta ctggtgatga tacaggaaaa attggcggcc
      781 ttattggtgc aaatgtttcg attggtcata cactgaaata tgttcaacct gatttcaaaa
      841 caattttaga gagcccaact gataaaaaag taggctggaa agtgatattt aacaatatgg
      901 tgaatcaaaa ttggggacca tatgatagag attcttggaa cccggtatat ggcaatcaac
      961 ttttcatgaa aactagaaat ggctctatga aagcagcaga taacttcctt gatcctaaca
     1021 aagcaagttc tctattatct tcagggtttt caccagactt cgctacagtt attactatgg
     1081 atagaaaagc atccaaacaa caaacaaata tagatgtaat atacgaacga gttcgtgatg
     1141 actaccaatt gcactggact tcaacaaatt ggaaaggtac caatactaaa gataaatgga
     1201 tagatcgttc ttcagaaaga tataaaatcg attgggaaaa agaagaaatg acaaattaat
     1261 gtaaattatt tgttcatgta caaataaata taatttataa ctttagccga taacttcaga
     1321 aatgatgttt ttcggctaat tttt
//
LOCUS       DALAAR010000193         1335 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000193 DALAAR010000000
VERSION     DALAAR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.201"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            87..1214
                     /locus_tag="O2458_002875"
     CDS             87..1214
                     /locus_tag="O2458_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCZ3888689.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT          461 a          180 c          285 g          409 t
ORIGIN      
        1 cagaattttt tgtaaataat aatattaaag tgtaaaataa cagataagaa tagacttaat
       61 gtatataaat taaaggagtg ttggaagtga aaactagagc agcagtttta catgaaatgg
      121 gggctaaaaa gccctataaa gaatctaaac ctttaaaaat tgaatactta gaacttgata
      181 atcctagcga acacgaagta ttaattaaaa ttcatgcagc tggattatgt cactctgatt
      241 tgtcagtaat taatggtaac agacctagac ctttacctat ggcacttggt catgaagctt
      301 ccggtgaagt aattaaagtt ggaaaagctg ttacaagagt tagcgaagga gatcatgtgg
      361 tatgcacatt tattcccagt tgtggaaaat gtatcccatg taaagaagga cgtcctgcat
      421 tatgtgaaaa cggagcaata tctaatgaaa aaggcgaaat gttagaggga gggatgcgtt
      481 tatctaatga tgaaggaaaa gtatatcatc acttggggat atcaggtttt gctgaatatt
      541 ctgttgtctc tgaaaactct atagttaaaa ttgataaaaa aatacctttt gaacgtgcag
      601 ctgcatttgg ttgtgctatt atcacaggta tcggtgctgt ggtgaataca gcccaaattc
      661 gttctggtag taatgtagcg gttgttggtt taggaggtat tggattgaat gctattattg
      721 gagctaaact agcgggagcc aatgaaatta ttgctttaga tattaacgaa gataaatttg
      781 aattagcaaa gcaatttggg gctacagcaa catttaattc aagcgataaa gatatcgatg
      841 agcaaattaa agaatatatt cctggcggag tagaatatgc ttttgaaaca gcgggtgtag
      901 tgccagctat gaaagttgct tatcagatta ctaaacgagg ggggacaact gtaacaacag
      961 gactgcctaa tcctaaagat aatttttctt tccctcaagt tactttagcg gctgaagaac
     1021 gtaccattaa aggatcatat gtaggaagtt gtgtacctga cagagatata ccaaggttcg
     1081 ttaatctata caaccaagga cgtttaaata ttgattcact catcagtgag gttatcactt
     1141 tagatgaaat taatgaggga tttgatcgtt tgtctaatgg tgaagtaggt agaattataa
     1201 tgaagatgca ttgaatataa tagaattcaa gtcgttcctc ctcttgattt ctatgaaaag
     1261 aataacaatt tgagtaggaa gattactgac aaatattaca tttgcattgt gactgccaat
     1321 ctaattgtat tttta
//
LOCUS       DALAAR010000194         1327 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000194 DALAAR010000000
VERSION     DALAAR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.202"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            140..775
                     /locus_tag="O2458_002876"
     CDS             140..775
                     /locus_tag="O2458_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ3888690.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            741..986
                     /locus_tag="O2458_002877"
     CDS             741..986
                     /locus_tag="O2458_002877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888691.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            1010..>1327
                     /locus_tag="O2458_002878"
     CDS             1010..>1327
                     /locus_tag="O2458_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3888692.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICC"
BASE COUNT          480 a          146 c          216 g          485 t
ORIGIN      
        1 tttgtttgta atatttttgt ttttcttatt tttaaatatg tgctcactat atgacaataa
       61 agttcatgta ttatttaaat caaatggaat tgaaaagttt taataggaat aataaacaaa
      121 ggaatggtag tgataaatat tgctatgtga atctaaagtt atcaacaaaa atcctaagta
      181 tagagttatt aaatatggtg atgaatattt aatgattgat ttagtaagta cctggttaac
      241 tttatttctt cctatgatta attggttaat tccaaaaaaa tatgtcaaaa tcagtaaaaa
      301 agaatttgac gatttaaaca ttgtcaaacc tgttaaaaat aaagcttttt ggccagttgc
      361 aggtagtact attttgttcg gagttacgtt tagaaaatat attccttcac ttaatattca
      421 attagagaaa aacatggtga ttgtaatatg ttgtgcaata tttctgggtg ttttaatact
      481 ttttttattt ctgaatcgta agctaaggtt ggaaatttat aataataact ctagtaaagg
      541 gaaaataatt ttatttcctt cattaaaaaa cttttgtttc acaatatttt attatttttt
      601 atttggcggt ctttcaataa tggctctaag tatgttatta actttaaatc ctcaaaatat
      661 aataggcttt attggttggt tggtaatgac tgcaggtttc tttctgttaa acatgtcatc
      721 gattattgac aaaaaaattt atgtattatc taaaactaac acggtggaaa aatgatggtt
      781 tagctggatt tactgcaggt tctatttcgg caatacttgt atattggacc aatcaaaaaa
      841 atgaatttgg aataaaagat aaaaacgatt ggataggaca taaactagac gttggtatag
      901 atgctgtaga aaaatctgca gaaaaaacag tagatggtgt tgaaaatgtc atggtgaagc
      961 ttcaaaaagt atttctaatc atataagccc taagaaatgg agctggtaaa tgttgctatg
     1021 cgaatctaaa atcatcaata aaaacccaaa atatagaatt attaaatata atgatgaata
     1081 cttaatggtc gatataataa gcacttggat tagtttattt tttcctttta ttaattggtt
     1141 catcccaaaa gaatacgtca aaattagtag agaagagttt gaaaacttaa atattgttaa
     1201 acctgctaaa aagaatgttt tttggccagt tgcaggtagc tctgctttgc tgggagttgc
     1261 attaagaaaa tatacacatt tacttgacat tcaacttgat aaaaaattag ttattgccat
     1321 atgttgc
//
LOCUS       DALAAR010000195         1326 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000195 DALAAR010000000
VERSION     DALAAR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.203"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(353..1138)
                     /locus_tag="O2458_002879"
     CDS             complement(353..1138)
                     /locus_tag="O2458_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCZ3888693.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT          459 a          232 c          197 g          438 t
ORIGIN      
        1 tttttcataa ttaataacat ccccaaaaat agattgaaaa aataactgta aaacattccc
       61 ttaataataa gtatggtcgt gagcccctcc caagctcgcg gccttttttg taatgaagaa
      121 gggatgagtt aatcatcatt atgagacccg ccgttaaaat atatgaataa gtctaatgtt
      181 ggaaaaggtc aaaaaattaa tcaatttaat taagaaaatc attcatttgc aaagggcgaa
      241 atgggttctt actgagttat ctattataaa aaaataaaca tagacttatg aaaaatctct
      301 cataaatcta tgtttagtca tgacatgtgt taaatattat ttcgggcgct tcttatttat
      361 acaaatctaa tttaatactt ttaaatacag gtatattttc gcgttgctgt tctacttcat
      421 ttaagtttaa atctacagtc aaaatatctg cggattcatt taattctcca actaaatctc
      481 catttgggtt tataactatc gaatgaccag catattctgt gttaccatcg aatccagtgc
      541 tattagttcc aatgacaaac atattatttt caattgcacg tgcctttagt aatgaatgcc
      601 aatgttgaag acgtgacata ggccattgcg ccacataaaa tgcaatttta gcaccactac
      661 gagcaggata tcttaataat tctggaaaac gtaaatcata acagataagt tgggtcacat
      721 aagtaccgtc agacaattga aagggttcag ctacgtattc gccagcggtt aaaaattcat
      781 gctctcttaa cataggaact aaatgaactt tgtcgtattc attaatcagc tggccacttt
      841 tattcacact aaaagctgta ttaaatattt gattgtttct aatgttagaa actgacccag
      901 ctacgatatc gactttatat ttttcagcta aatgtttaat aaatgaaaaa ctttgtccta
      961 gattattatc tgctttttca tttaaatgct ctaaatcata gccattattc cacatttcag
     1021 gtaaaacgac tacatctact tcagcattca tatttttttc gaaccattgc gttatttgag
     1081 tttcattttt agaactatct ccaaaaacaa tcggtaattg ataaatttgg actttcataa
     1141 catcacatcc ttgatagatc ttatatataa cttactaaaa gttatgttga aacgcaaaaa
     1201 acgagcacaa gacataaaat caaagtccta ggctctacaa agttatattg acagtagttg
     1261 atggggcccc aacatagaga aattggaaca ccaatttcta cagacaatgc aagttggggt
     1321 gggctc
//
LOCUS       DALAAR010000196         1254 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000196 DALAAR010000000
VERSION     DALAAR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.204"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..>1254
                     /locus_tag="O2458_002880"
     CDS             <1..>1254
                     /locus_tag="O2458_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ3888694.1"
                     /translation="YLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKI
                     SENEKLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSK
                     KLRDENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFII
                     IDALNEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVF
                     QHEGFSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQT
                     ISVAELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESY
                     TVVNNIAKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEK
                     YRNVDKRDLVTKLQSDEKVTRYFQKE"
BASE COUNT          514 a          125 c          196 g          419 t
ORIGIN      
        1 tatttggaac tggaagaaga gtttgaagaa aagaaaaaat acttaagtga aatttatgaa
       61 ttgatagacg aaattaatat tgacccttat atttttctga cagaacataa tgaatgtaat
      121 atatacaaaa taagtgagaa tgaaaaatta gaacttcaaa cttatatgag taaaatcgaa
      181 aaagtattat taaaatatca aacgtattta aaagaaacat gtaaagaatg cttgttttat
      241 ccttatttgc tagttcaagg tgaagctggc ataggaaaat cacacctttt agcgcatcta
      301 tctaaaaaac ttagagatga aaatcatatt atttatctgt ttttgggaca attttttact
      361 aaaaatgaag atccatggca tcaaatactt aatgatttag aagttacaaa ttctgttgat
      421 aattttttaa ggtcaataag taataaggcc aaagaaacaa aaaaaagagc ttttattatt
      481 attgatgcgc ttaatgaagg tgaaggtaaa aggttatggg gaaattattt tcaaagcttt
      541 atcaaccata taaagaagta ttctaatata gctttaatat tttctattag aacccctttt
      601 gaagatgtta tattacccaa aaacgcaata caagataaca atattgtagt atttcagcat
      661 gaaggtttta gtaaggaaga aaactataat ccaattgtat ctttttgtga tttttatgga
      721 ttagagctac ctaagttacc tatattaaat ccagaattca acaatccatt atttttaaaa
      781 ctaatgtgtg agtactgtgt aaataaattt aaggaatttg accaaactat aagtgttgca
      841 gagctattta caaatgttct taagacagta aatattaatt tatctaagga agataaattt
      901 gattttgata aaaatattaa tgttgtacaa aaagtaataa aaggattagt tgaattaatg
      961 aacgactcag aatttaatca actaaattat gaggaatcct atactgttgt taataatata
     1021 gctaaagagt atgttcaaaa gtcaaataga tttttagaag cgctaataga tgaaaatata
     1081 ttaattaaaa atactggcta taaaggtgaa atgattattt atttttcgta tgagcgtatg
     1141 ggagattact ttttatcaga atacctttta gagaaatata gaaatgttga taaaagagat
     1201 ttagtaacta aattacagtc agatgaaaaa gtgacaaggt attttcaaaa ggaa
//
LOCUS       DALAAR010000197         1235 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000197 DALAAR010000000
VERSION     DALAAR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.205"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..>1235)
                     /locus_tag="O2458_002881"
     rRNA            complement(<1..>1235)
                     /locus_tag="O2458_002881"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          281 a          356 c          238 g          360 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccaga ttccgacgga
     1021 atttcacgtg ttccgtcgta ctcaggatcc actcaagaga gacaacattt tcgactacag
     1081 gattattacc ttctttgatt catctttcca gatgattcgt ctaatgtcgt cctttgtaac
     1141 tccgtataga gtgtcctaca accccaacaa gcaagcttgt tggtttgggc tcttcccgtt
     1201 tcgctcgccg ctactaaggg aatcgaattt tcttt
//
LOCUS       DALAAR010000198         1210 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000198 DALAAR010000000
VERSION     DALAAR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.206"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(242..775)
                     /locus_tag="O2458_002882"
     CDS             complement(242..775)
                     /locus_tag="O2458_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCZ3888695.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            866..>1210
                     /locus_tag="O2458_002883"
     CDS             866..>1210
                     /locus_tag="O2458_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ3888696.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSI"
BASE COUNT          453 a          152 c          163 g          442 t
ORIGIN      
        1 tatttctagt tgaaatttca aattgcgggc attcattttc aaaatagtaa agaatttaat
       61 agatgaaaac ctttgaatca tactagttag tatggattga ttaatatttt aacctgctaa
      121 atgagtagct tatgtatttt aaatagctgt catctagcag gttctttatt attaataagt
      181 tcagataaat tattttagtc tgttttctaa tgttgataat aataacttta aatctgtact
      241 ttcattgtga gatatatagt acatcataaa tttttatatt tggattgaga ctctttggaa
      301 gattttcaat aaatgaatat ttcacaaact tccacttatc aggattataa attataattt
      361 ctgcaactgc attatcgaag attctaaaca attttttgat ttctatttct tttagagata
      421 aagtagggaa aatgtcgata tattcttcaa gcgcataaag actatgttta acagaatcgg
      481 ttatgttcat tgaatatatc acaccaatat ttatattttg ccatccaaaa gaagaaataa
      541 cattatcata aaatccattg aatataaatt cagtcatccc ctctgaactt ctccataaat
      601 ataatggtga ataagtattt gtaatgcaat tagtagttgt ttgacttatt aaataagctt
      661 ttataattaa atttttaaat ccatcagtct tataaccatt taatcgaact ctatctctaa
      721 ttatttccat attgtaatca ctaggcaact taacatgata ttgcattgca tgcatttagc
      781 ataccccctt ttataaaaag gatagcaata ataagtaaaa tctcatatta tccaattgtg
      841 atatagttat cataaaaagt gataggtgat taaattgaac tttaatgatt tggaaatttt
      901 tataactgta tgtgaagaag catctatcaa taaagctgca attaaactta gatatgcaca
      961 atctaatata tctcaaagaa ttagcaagct tgaaaatgaa ttaggtgtag ttttgctttt
     1021 tagaaatcaa aaaggtgcta aggcaactaa agcaggcgaa gaattcttag cgtatagcaa
     1081 aaaagtatta agagatacag agactataaa aaataaaatg aaaaataata ctatgtctat
     1141 tttatgctca gaactgttat ttaattattt atctgagagc gaagaaatta tgatgtcgaa
     1201 taactcaatt
//
LOCUS       DALAAR010000199         1155 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000199 DALAAR010000000
VERSION     DALAAR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.207"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..338
                     /gene="lpl8"
                     /locus_tag="O2458_002884"
     CDS             <1..338
                     /gene="lpl8"
                     /locus_tag="O2458_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ3888697.1"
                     /translation="IENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDE
                     NVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQ
                     PAKVDEDDE"
     gene            357..1142
                     /gene="lpl9"
                     /locus_tag="O2458_002885"
     CDS             357..1142
                     /gene="lpl9"
                     /locus_tag="O2458_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCZ3888698.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
BASE COUNT          483 a          134 c          219 g          319 t
ORIGIN      
        1 gaatagaaaa ctttaaattt tttggacaat atgcaaacct taaagaattg aaaaactaca
       61 acaatggtga tgtctcaatt aatgagaatg ttccaagtta tgacgcaaaa tttaaaatga
      121 gcaataaaga tgaaaatgtt aagcaattaa gaagtcgtta taatattcct actgataaag
      181 caccggtatt aaaaatgcat attgatggta atttgaaagg aagttctgtg ggttataaaa
      241 agttggaaat tgacttttca aaaggtggaa aaagcgattt gtcagtaata gattctttga
      301 atttccagcc ggcgaaggta gatgaagatg atgaatgatg aggatggtgt gtaacaatga
      361 agtctataaa aaggattgga ttgtgcatta gtttgttgat tttaatcatc tttgttacat
      421 cttgtgatgg tgataataag atcattggag attcaaaaga agaacaaatc aaaaagagct
      481 ttgcgaaaac gttagatatc taccctatta agaatctcga ggatttatac gacaaagaag
      541 gatatcgaga tggcgaattt aaaaaagatg ataaaggtac ttggctaatt agatctgaaa
      601 tgaaaatcca attaaaagga gaaaatctgg aatctagagg agcagtttta gaaattaaca
      661 gaaatactag aacggctaaa gggcattata ttgttagaga agttgttgaa gatagcgatg
      721 gaatgacaca caatcataca aaaagatatc ctgtgaaaat ggaaaataat aaaatgattc
      781 cattaaaacc aatcgatgac gaaaaagtaa aaaaagaaat cgaagaattt aacttctttg
      841 tacaatacgg gaatttcaaa gaattggaaa actataaaga ggacgaagtg tcatataacc
      901 cagaagtacc aatttactct gcaaaatatc aattgaaaaa cagtgattac aatgttgaac
      961 aattaaggaa gcgatataat atcccgacgc aaaaagcgcc caaattatta ttgaaaggct
     1021 caggtaattt aaaaggttca tcagtcggat ataaaaatat tgaatttacc tttattgaaa
     1081 acaaggaaga aaatatttac ttcacagata gtatctactt taatccaagc gaggataaat
     1141 aaatataatt actaa
//
LOCUS       DALAAR010000200         1114 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000200 DALAAR010000000
VERSION     DALAAR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.208"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            99..668
                     /gene="agrB"
                     /locus_tag="O2458_002886"
     CDS             99..668
                     /gene="agrB"
                     /locus_tag="O2458_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCZ3888699.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            665..805
                     /locus_tag="O2458_002887"
     CDS             665..805
                     /locus_tag="O2458_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCZ3888700.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT          381 a          141 c          153 g          439 t
ORIGIN      
        1 acatttaaca gttaagtatt tatttcctac agttaggcaa tataatgata aaagattgta
       61 ctaaatcgta taatgacagt gaggagagtg gtgtaaaatt gaattatttt gataataaaa
      121 ttgaccagtt tgccacgtat cttcaaaaga gaaataactt agatcatatt caatttttgc
      181 aagtacgatt agggatgcag gtcttagcta aaaatatagg taaattaatt gttatgtata
      241 ctattgccta tattttaaac atttttctgt ttacgttaat tacgaattta acattttatt
      301 taataagaag acatgcacat ggtgcacatg caccttcttc tttttggtgt tatgtagaaa
      361 gtattatact atttatactt ttacctttag taatagtaaa ttttcatatt aactttttaa
      421 ttatgattat tttaacagtt atttctttag gtgtaatctc agtatatgct cctgcagcaa
      481 ctaaaaagaa gcccattcct gtgcgactta ttaaacgaaa aaaatattat gcgattattg
      541 ttagtttaac ccttttcatt atcacactta tcatcaaaga gccatttgcc caattcattc
      601 aattaggcat cataatagaa gctattacat tattacctat tttctttatt aaggaggact
      661 taaaatgaat acattattta acttattttt tgattttatt actgggattt taaaaaacat
      721 tggtaacatc gcagcttata gtacttgtga cttcataatg gatgaagttg aagtaccaaa
      781 agaattaaca caattacacg aataatttaa atagagagtg tgatagtagg tggaattatt
      841 aaatagttat aattttgttt tattcgtatt aactcaaatg atattaatgt ttacaatacc
      901 agctataatt agtggtatta agtacagtaa acttgattat tttttcatca tagtaatttc
      961 gacattatcg ttatttctat ttaaaatgtt tgatagcgcg tccttaatca tattaacttc
     1021 atttattatt ataatgtatt ttgtcaaaat caaatggtat tctattttgt tgattatgac
     1081 ttcgcagatt attctatact gtgctaacta catg
//
LOCUS       DALAAR010000201         1093 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000201 DALAAR010000000
VERSION     DALAAR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.209"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            10..85
                     /locus_tag="O2458_002888"
     tRNA            10..85
                     /locus_tag="O2458_002888"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Val,seq:tac)
     gene            102..177
                     /locus_tag="O2458_002889"
     tRNA            102..177
                     /locus_tag="O2458_002889"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:135..137,aa:Thr,seq:tgt)
     gene            183..258
                     /locus_tag="O2458_002890"
     tRNA            183..258
                     /locus_tag="O2458_002890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Lys,seq:ttt)
     gene            262..343
                     /locus_tag="O2458_002891"
     tRNA            262..343
                     /locus_tag="O2458_002891"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Leu,seq:tag)
     gene            347..421
                     /locus_tag="O2458_002892"
     tRNA            347..421
                     /locus_tag="O2458_002892"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:379..381,aa:Gly,seq:gcc)
     gene            432..520
                     /locus_tag="O2458_002893"
     tRNA            432..520
                     /locus_tag="O2458_002893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Leu,seq:taa)
     gene            539..612
                     /locus_tag="O2458_002894"
     tRNA            539..612
                     /locus_tag="O2458_002894"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Arg,seq:acg)
     gene            622..695
                     /locus_tag="O2458_002895"
     tRNA            622..695
                     /locus_tag="O2458_002895"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:Pro,seq:tgg)
     gene            713..788
                     /locus_tag="O2458_002896"
     tRNA            713..788
                     /locus_tag="O2458_002896"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Ala,seq:tgc)
     gene            810..883
                     /locus_tag="O2458_002897"
     tRNA            810..883
                     /locus_tag="O2458_002897"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:844..846,aa:Met,seq:cat)
     gene            912..985
                     /locus_tag="O2458_002898"
     tRNA            912..985
                     /locus_tag="O2458_002898"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="O2458_002899"
     tRNA            995..1084
                     /locus_tag="O2458_002899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
BASE COUNT          244 a          259 c          299 g          291 t
ORIGIN      
        1 ttttaaatcg gagaattagc tcagctggga gagcatctgc cttacaagca gagggtcggc
       61 ggttcgaacc cgtcattctc caccatttat tcttacatat tgccggccta gctcaattgg
      121 tagagcaact gacttgtaat cagtaggttg ggggttcaag tcctctggcc ggcaccatgg
      181 aagagccatt agctcagttg gtagagcatc tgacttttaa tcagagggtc agaggttcga
      241 atcctctatg gctcaccatt tgcgggtgtg gcggaattgg cagacgcact agacttagga
      301 tctagcgcct tacggcgtgg gggttcgact cccttcaccc gcatatgcag aagtagttca
      361 gcggtagaat acaaccttgc caaggttggg gtcgcgggtt cgaatcccgt cttctgctcc
      421 atttttatag tgccggggtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggtg
      481 agtgatcacc gtaccggttc gattccggtc ctcggcacca ttttcaataa aaacatatgc
      541 gcccgtagct caattggata gagcgtttga ctacggatca agaggttatg ggttcgactc
      601 ctatcgggcg cgttaattat acgggaagta gctcagcttg gtagagcact tggtttggga
      661 ccaaggggtc gcaggttcga atcctgtctt cccgatatac tgtaattatt atgggggctt
      721 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagt
      781 ctccaccata ttatttacaa actatataag gcggtgtagc tcagctggct agagcgtacg
      841 gttcataccc gtgaggtcgg gggttcgatc ccctccaccg ccactattta ttagttgtaa
      901 aattatattt aggaccttta gctcagttgg ttagagctaa cggctcataa ccgttcggtc
      961 gcaggttcga gtcctgcaag gtccatataa ttttggagga atacccaagt ccggctgaag
     1021 ggatcggtct tgaaaaccga caggggctta acggctcgcg ggggttcgaa tccctcttcc
     1081 tccgttttac taa
//
LOCUS       DALAAR010000202         1043 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000202 DALAAR010000000
VERSION     DALAAR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.210"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..774
                     /locus_tag="O2458_002900"
     CDS             <1..774
                     /locus_tag="O2458_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCZ3888701.1"
                     /translation="KVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLT
                     QTGTNTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAK
                     ALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT          415 a          179 c          190 g          259 t
ORIGIN      
        1 aaagttactt tattaaatga aaaaggcgaa gtaattggaa caactaaaac agatgaaaat
       61 ggtaaatact gctttgataa tttagatagc ggtaaataca aagttatttt tgaaaagcct
      121 gctggcttaa cacaaacagg tacaaataca actgaagatg ataaagatgc agatggtggc
      181 gaagttgacg taacaattac ggatcatgat gatttcacac ttgataatgg atactacgaa
      241 gaagaaacat cagatagcga ctcagattcg gacagcgact cagattcaga cagagactca
      301 gactcagata gtgattcaga ctcggatagc gattcagatt cagacagcga ttcagattca
      361 gatagcgatt cagattcaga cagagactca gatagtgatt cagactcaga tagcgactca
      421 gattcagaca gcgactcaga ttcggatagc gactcagatt cagacagcga ctcagactcg
      481 gatagtgatt cagactcaga tagcgactca gactcagata gcgattcaga ttcagatagc
      541 gactcagact cagacagcga ttcagactca gacagcgact cagactcaga tgcaggtaag
      601 cacacacctg ttaaaccaat gagtactact aaagaccatc acaataaagc aaaagcatta
      661 ccagaaacag gtaatgaaaa tagcggctca aataacgcaa cgttatttgg cggattattc
      721 gcagcattag gatcattatt gttattcggt cgtcgtaaaa aacaaaataa ataatacagc
      781 ttataccagg tccgtgaggc ctggtttttt atttcaaaat ttatgaaatg tatcttatgt
      841 ttgagattaa atagtgcaat tttttaaaaa atataacata aatcaacata aatttaattc
      901 atctttacat taaaagtaca aacgaataag aaaagcaatt aaacacagta tcaaaccatt
      961 taaaacaatt gatataaaaa acaaacgaat aattaaaaag tactagaatt aatttattca
     1021 aaccaaagtg taaaataaca gtc
//
LOCUS       DALAAR010000203         1025 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000203 DALAAR010000000
VERSION     DALAAR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.211"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(238..669)
                     /locus_tag="O2458_002901"
     CDS             complement(238..669)
                     /locus_tag="O2458_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ3888702.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          333 a          158 c           98 g          436 t
ORIGIN      
        1 attcaacttt gcaacagaac ctatattttg ctataattat taaattaatt gcctttgtgt
       61 tagtattccc tggattacta acactttggt tagctgttct aagtgataca ggtgcagcaa
      121 tattagttat attaaattca ttacgtttat taaaaaaata aaaaagccta aatactctct
      181 cgatttgtga gaaagtattt aggctttttg aattaataaa tttcttactt cttatgttta
      241 tttaacaatt agtactggga cagaacttct tttagcaatc ttatgtgtaa catctcctaa
      301 aacatgttta atattcaaat cgactctatg attactcata attactaaat caaattcacc
      361 attatttata tctccagaag taattttttc taagattgta tctttaatat gcccaaaatc
      421 tacatctatt ttatattgaa tatctctttt ttctaattca cgtaaaaatg gagtgagttt
      481 tttctctttt tctctaataa tatcttcttt atgcttatta taatacttga cactaattgc
      541 aagatcattt tctgttacaa cgtgataaat tacaacaact gaatcttcat cggtgacttt
      601 ttctaaatag tcagggatag cactaaaatc attttcgaaa tcatagggta ataatatatt
      661 tttgaacatc tacaccagcc tcctattact tattatttag atgtctatta cttatatata
      721 ccctaaatat gtatgcttat tctcatattc aaattattca aatatattcc ttatcatgta
      781 ttagattcta ttttttatat tatttttgta attctttgta taaacctatt tatttataaa
      841 gcgctctaat acatacctaa atatcttcaa cttataaact actttttatt tcaatgcctt
      901 tttccaaact tcttttatga gatattgtgg ttattacctg acatttacaa tttacacatt
      961 tcgctattat tacgcaaatt attttgattt tataattatg atgactataa tctattctaa
     1021 ttaat
//
LOCUS       DALAAR010000204         1007 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000204 DALAAR010000000
VERSION     DALAAR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.212"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            84..836
                     /gene="sarS"
                     /locus_tag="O2458_002902"
     CDS             84..836
                     /gene="sarS"
                     /locus_tag="O2458_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCZ3888703.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          401 a          147 c          123 g          336 t
ORIGIN      
        1 atttttaatt gtaaattatg gattattttt cttattatat tgtgttatat taaaataaag
       61 tgcatataca aggagataaa acaatgaaat ataataacca tgacaaaatt agagatttta
      121 taatcattga agcatatatg tttcgtttta agaaaaaagt caagcctgaa gtcgatatga
      181 ctataaaaga atttatatta ctgacttatt tatttcatca acaagaaaac acacttccat
      241 ttaagaagat tgtttcagat ttatgttata aacaatcgga tttagtacag catataaaag
      301 tacttgtgaa acattcatat attagtaaag ttcgaagtaa aattgatgag cgtaatactt
      361 acatttcaat atctgaagaa caacgagaga aaattgcaga acgtgttaca ttgtttgatc
      421 aaatcattaa acaatttaac cttgcagatc aaagtgaatc acagatgata ccaaaagata
      481 gtaaagaatt tctaaacttg atgatgtata caatgtattt caagaatatt atcaaaaaac
      541 atctaacatt aagttttgta gaattcacaa ttctagctat tatcacttct caaaataaaa
      601 acatcgttct tcttaaagat ttaattgaaa caatccacca taaataccct caaactgtta
      661 gagctctcaa taatttaaaa aagcaaggct atctaataaa agaacgctca actgaagatg
      721 aaagaaaaat tttaattcat atggatgacg cgcagcaaga ccatgctgaa caattattag
      781 ctcaagtgaa tcaattatta gcagataaag atcatttaca tcttgttttt gaataatatc
      841 tctattacgc aagtgtgctg tattctaaag tgcacttgtg ttttctattt tttaataaaa
      901 cctcagcaca taatgaacaa ctttctattt tctatatcac ttaaaaccat ttccgaaatt
      961 aaacctcagc acattcaaag ccccacttta ttcttaaaaa tattttt
//
LOCUS       DALAAR010000205          997 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000205 DALAAR010000000
VERSION     DALAAR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.213"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            9..275
                     /locus_tag="O2458_002903"
     CDS             9..275
                     /locus_tag="O2458_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888704.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            419..727
                     /locus_tag="O2458_002904"
     CDS             419..727
                     /locus_tag="O2458_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888705.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(835..>997)
                     /locus_tag="O2458_002905"
     CDS             complement(835..>997)
                     /locus_tag="O2458_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888706.1"
                     /translation="KISLAYNLAIIIGSLIILCILISRGYDIYVILIPILTILASLIN
                     LFCDIKKHK"
BASE COUNT          363 a          160 c          121 g          353 t
ORIGIN      
        1 gtgaaataat gaaaggctct aaacaaatac ttttgattat gggcattata tctcttattg
       61 ttttatttat ttttacacta ttcatcatgg cgcaatatgc aaaacattat gaacaaaaat
      121 ccgacagttc caacgcacat actttaaatt catcatcagc cataattgag caacatacta
      181 tgtcgaattt agcgtctcta gatttatacg ctcctgttcg taacattact tctagtcgtg
      241 atattcatcc catttatttc atgacaaaag attaaaactt cctttttcat aaccccattc
      301 attcaactaa taaattaaaa aatgataaaa acgtactgtt ttttagaaac atacatatat
      361 aagaaacgca tccattacta aaagacagta cattatattc tatcaggaag gtgcaacaat
      421 gaccgtcaac atcgtattta gtatcgtatt ctgcataagt atggttatat taggcattta
      481 tgtcgcaata actaaagatt tcacactaat ctcttttata aatcaaacgg ccattgcaga
      541 taaacacaag aaccaaattg catatatatt tacgctatgt ataagtttga gtgcagtatt
      601 tttaatgtct tcaattctaa gctttgaata tgattttatt gcattagcat tcttattttt
      661 aacgatagca ttattactta tcgcattatt ctacgtttgc ttttataaaa ttacaaaata
      721 cccataaatg tttgtgatag tgtgttggtt agatttattg atgcgctgtc agtaagtaat
      781 tgtttgcgaa aaaaatacaa ccttgtacga atacaaagtt gcattaatat agaatcattt
      841 atgcttttta atatcacaaa acaaattgat taaactagct aaaatagtca aaattggaat
      901 caagattaca taaatatcat aacctctaga tattagtata cataatataa ttagcgaccc
      961 tattataatt gctaaattat aagccaaact aatttta
//
LOCUS       DALAAR010000206          963 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000206 DALAAR010000000
VERSION     DALAAR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.214"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..>963)
                     /locus_tag="O2458_002906"
     CDS             complement(<1..>963)
                     /locus_tag="O2458_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ3888707.1"
                     /translation="TAEKYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLK
                     LFENNNDPYIVERLYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYA
                     RQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSI
                     DKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDA
                     KLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRD
                     IDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFL"
BASE COUNT          328 a          151 c          104 g          380 t
ORIGIN      
        1 tctaaaaata attttgtttc atcaaaaact ttggtatttt ttacccattc attcgattga
       61 tgcgaaggaa gtggtgaggg tactagtatt tttgattctt tatcaatttc aagtaatagt
      121 aaagtaggat caatatctct aatataatca tcccatagtc cagaatattg aacatcagga
      181 aatttatcta caagtttagc taaaatttca tagtaagcaa tccattgata ttttttgcta
      241 attctttcaa ttgaattatt aaagttatca taacgattaa cccacatatc aaattcacca
      301 tgtaattttg catcataacc catttcaaaa atcctcatta atgctatatt acttaagtct
      361 tgatcacttt taaaaccctt tacccagttt cgtacagcat aaccaaatac atatctgcca
      421 aaatcgccat atgccccagt tcctcttcca tattccgttg tcattgagtt tttaatttta
      481 tcgatagaaa aatgtaaatg gctatctaat ttgtttttta aagattttat gtaatcgtct
      541 atatttaaat tcgaatattc ttttttatac caattacttt tataaggtgg cctaattttt
      601 tctaaattta tattagatat atcttttgat aaactaatat attcaattgt ttgcctagca
      661 taatctctca ttaaaatatg aggatatacc atttctttat caaatatttc ttcataaata
      721 taattagcaa tctctatatg tatttcacaa atgtcgattc gaagtgtcgc ccctaaaaca
      781 gaagcatata atctttcgac tatatatgga tcattgttat tttcaaataa ttttaataaa
      841 ccaataattt tagttggaaa tgttttgaat aattttacta aagatttagt accgagatct
      901 cttaatttat tattagttgc agaaaatatc cacgttaaaa aaatcatata tttttccgca
      961 gtt
//
LOCUS       DALAAR010000207          938 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000207 DALAAR010000000
VERSION     DALAAR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.215"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(65..148)
                     /locus_tag="O2458_002907"
     tRNA            complement(65..148)
                     /locus_tag="O2458_002907"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            complement(187..261)
                     /locus_tag="O2458_002908"
     tRNA            complement(187..261)
                     /locus_tag="O2458_002908"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Gly,seq:tcc)
     gene            complement(365..439)
                     /locus_tag="O2458_002909"
     tRNA            complement(365..439)
                     /locus_tag="O2458_002909"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(404..406),aa:Gly,seq:tcc)
     gene            complement(447..520)
                     /locus_tag="O2458_002910"
     tRNA            complement(447..520)
                     /locus_tag="O2458_002910"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(486..488),aa:Cys,seq:gca)
     gene            complement(526..597)
                     /locus_tag="O2458_002911"
     tRNA            complement(526..597)
                     /locus_tag="O2458_002911"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Gln,seq:ttg)
     gene            complement(609..681)
                     /locus_tag="O2458_002912"
     tRNA            complement(609..681)
                     /locus_tag="O2458_002912"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(646..648),aa:His,seq:gtg)
     gene            complement(684..757)
                     /locus_tag="O2458_002913"
     tRNA            complement(684..757)
                     /locus_tag="O2458_002913"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(723..725),aa:Trp,seq:cca)
     gene            complement(773..853)
                     /locus_tag="O2458_002914"
     tRNA            complement(773..853)
                     /locus_tag="O2458_002914"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Tyr,seq:gta)
     gene            complement(859..934)
                     /locus_tag="O2458_002915"
     tRNA            complement(859..934)
                     /locus_tag="O2458_002915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(899..901),aa:Thr,seq:tgt)
BASE COUNT          272 a          226 c          217 g          223 t
ORIGIN      
        1 accaaaagaa accttgtttt acaaggtttc taatacgtta tgttatgtaa ataacagtta
       61 attataccgg tggtcggggt cgaaccgaca ctccacaagt ggaacgggat tttgagtccc
      121 gcgcgtctgc caattccgcc acaccggctt aatggtaaac aaaaaacttc cctttggaag
      181 caattatgga gcggaagata ggatttacac ctatacctcg ttccgggaag gaacgtgttc
      241 taaaagttga actactcccg caaatattaa attatggagc ggaagatagg atttacacct
      301 atacctcatt ccaggaagga atgtattcta agagttgaaa tactcccgca ttattattaa
      361 attatggagc ggaagatagg atttgcacct atacctcgtt ccgggaagga acgtgttcta
      421 aaagttgaac tactcccgca taaacctgga ggcggcaacc ggatttgaac cggtgataaa
      481 ggttttgcag acctctgcct taccacttgg ctatgccgcc aataactggg ctagctggat
      541 tcgaaccaac gagtgacgga gtcaaagtcc gttgccttac cgcttggcta tagcccatta
      601 ataataaggg cggctgaagg ggatcgaacc ctcgaatgtc ggaaccacaa tccgatgtgt
      661 taaccacttc accacagccg ccatggcagg ggcagtagga atcgaaccca caccaaaggt
      721 tttggagacc tctattctac cgttgaacta tgcccctatt aaaaataata aatggagggg
      781 ggcagattcg aactgccgaa cccgaaggag cggatttaca gtccgccgcg tttagccact
      841 tcgctacccc tccataaatg gtgccggcca gaggacttga acccccaacc tactgattac
      901 aagtcagttg ctctaccaat tgagctaggc cggctaag
//
LOCUS       DALAAR010000208          881 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000208 DALAAR010000000
VERSION     DALAAR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.216"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(181..723)
                     /locus_tag="O2458_002916"
     CDS             complement(181..723)
                     /locus_tag="O2458_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCZ3888708.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
BASE COUNT          281 a          180 c          111 g          309 t
ORIGIN      
        1 ccaccccaac ttgcattgtc tgtagaaatt ggcgttccaa tttctctatg ttggggcccc
       61 gcagtcaact actgccaata tagtgttgta gtgtctaaga cataaaagtt catctcagtc
      121 acaattactt tatagcctat ccatcatctt ttctacatga aatttttcaa gtgatatatt
      181 ttatttctca atattatcta aaaaatactg ttctacatct ttccagctat ttacgcgttc
      241 aaatcgatca tcattaatat tatgcactgc tgtaaacata atcggtgtac cagtaaaaat
      301 ttcaagctgt ctaggattgt catctattaa ataatcagct ttaacgatgt tttttctacc
      361 acaaaaaaca aaatgctgag gatctaaaaa tggaaagaac tctagtaacc attcatattt
      421 atcactaaat gatgttggta catccattgc tgctgtagca atatatacat cataatgttc
      481 agttaatttt ttcacaactt cttgtgcata cggcatcact ttaagatgtc tgaagaagcc
      541 tgattctctc aatacttctg taattaatcc atcatgttca ggaataacat gtttaagttt
      601 ttgaccattc aaagcttcca ttttaatacc taaatccgct ctaaaattga cagcatcaat
      661 gatttctcct aatgtatctg ccaatacttc atccatatca atcgcgattg atttacgggt
      721 cattttaatc ttctcctcaa aatatcagta ttgattcagt atagcaaaaa agcaattgat
      781 acaaaacaat atcatctatt gttgcttttt acactgaata ctctcacctc atcattcaca
      841 ctgcattgag cttgagacat aaatcaatgt tcaatgctct a
//
LOCUS       DALAAR010000209          879 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000209 DALAAR010000000
VERSION     DALAAR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.217"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            294..494
                     /locus_tag="O2458_002917"
     CDS             294..494
                     /locus_tag="O2458_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888709.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            571..702
                     /locus_tag="O2458_002918"
     CDS             571..702
                     /locus_tag="O2458_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888710.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
BASE COUNT          318 a          106 c          138 g          317 t
ORIGIN      
        1 tacttacaat tccattttgt atgtgctaaa ctgtttgtgt cagatgacat taaatagcat
       61 ctcctcgtgt tgattatttt ggttggctga ccaatattta ttctagcacg tagagatgca
      121 ttttttgtga caatggtaga accttttctg aaccatacgc atagcgtatg gttttctttt
      181 tacaaaaaac aaaagcagta aacctaaagt gtcgtaaggg tttactgctt ttataaaacg
      241 ttgttataac gtatattgtc ttttacgggc atataaaagg ggaatatttg aaaatgacca
      301 atccaacaag aacgaaacgt tgtggggggg atgttctata tggtattgat aatcattttc
      361 aactactatt atacattagt gagaatcatt gtcaattaga aactaaaact ttttttgaat
      421 attttttaag aatagtaaat aaaacgcatg attacgctat tttagaaaat aaaaaaattt
      481 gtatttctca ttagaattag aatatttaaa agtgatgagg tttaaacatt atattgttta
      541 catactcctt ttgaattcat acattatgaa atgttacttc caagttcaaa atcgcacatt
      601 gaaatgatgt gtgaaatgtt taaactacgg tcattttgtg aaaataaaga taataactat
      661 tcattttaca atagtgaaaa gtcagtatat gacaacaatt aatattgcgg taaggccttg
      721 tgttacagta ttctatattt aagtactgca atcagaatta acagaatgcc attaactgat
      781 tattaaatat ttgagttaat aaataattaa tgattgtagc ttgaaaaatt taaaacatgg
      841 ttattgattt gtgataaaat ttaaacgtaa acaaactaa
//
LOCUS       DALAAR010000210          859 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000210 DALAAR010000000
VERSION     DALAAR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.218"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(199..705)
                     /locus_tag="O2458_002919"
     CDS             complement(199..705)
                     /locus_tag="O2458_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888711.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
BASE COUNT          342 a          135 c           95 g          287 t
ORIGIN      
        1 tgaaattaac taattattaa ttaactataa acactttata atatagacaa aatatagcta
       61 tctatcttgt gactcatcag cacttataca ctagtcaaaa aattcatgcc tctacttttt
      121 cgaatttcaa aaataaaacc acacaacaaa aaagacacca tttgaatgtt ggtgtctgaa
      181 ggtataatct catagatttt atttgtaact tctcattcta tagttaaata ttgtgaccca
      241 gctaatgatt aacgttggta gaattactaa attcaaatta gacacgtcaa aaaagacaga
      301 ttggaaattg tcgatattaa tatcattaat gtccattttt tcatttactg cttgatagat
      361 cgcaaagtaa actaagaacc attgaattaa atgctctaaa acgacaacct ctgtcaaatc
      421 gacattcttt tttctaaaaa gtaatctaac tataaataat tggaatatac ttgaaatcaa
      481 gatcaatata atacccatta agaaaaaact accaaaatca attgtgataa attgaataat
      541 accataaata attacggcag atattgccag tgaaatatat cctataaaga taaataccac
      601 acccataaag agtgtcttta agaacagaaa aggcttcttt ctatttgtaa ccaaatagct
      661 caatatacaa agtagtaata gaattgttaa aattttaatt atcatcgttt gacctcaata
      721 tatttattta tatctttatt attgaccacg aaaaataaca atcacttaag tgattgccaa
      781 taatactcaa tgcctactct atagtaatct tggcacgtga ttttacacta ttttatctat
      841 ttaaaaatgt aagtgttga
//
LOCUS       DALAAR010000211          831 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000211 DALAAR010000000
VERSION     DALAAR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.219"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..294)
                     /locus_tag="O2458_002920"
     CDS             complement(<1..294)
                     /locus_tag="O2458_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888712.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFF"
BASE COUNT          329 a          106 c          112 g          284 t
ORIGIN      
        1 aaagaatata atagtaataa ttaaaagaat gctaaatatt aataattgat gatccaaagg
       61 tttatcaaag acttctttag aaaaaataaa tgccaatcta cctataaaaa aactaaccca
      121 agtcataata tgattaatga atgctttttt gccatgttct gatgttggtt catcagcaag
      181 tatccataca aataaaggta aaatgacagg tgcgaaaaag atacttaaat aactcaatgc
      241 tgataaaacg taactattgt catttttatg catattacta tttgtatttt ccactttttt
      301 caccttcttt acttttcttt actattataa aaacattaat agcgagctat tacaacagtt
      361 ataagagaag cttacgatat tgtaagtatg tgttgttata atcaatataa aattaagaaa
      421 gacctgagaa tttatattat catccacaat cgaagtgagt agcgaaattt attggaaaag
      481 gtacttatat tgaagatatt gttaatggtt catttttaaa aagccaagac gatttgaagt
      541 taaaagcttc tgacttcact gtcgaagata actataacta cggtgtactt atatttccag
      601 agaatctttc tcttacagat aataataaga aattggaatc tttaataaaa actatactat
      661 ttacaggtaa acgatatcaa tatacttatt ttaaaatttg atcttaatag ataaggatat
      721 aatgatttaa acaatttaag agatattatt ttcacaaaaa aagaatgctt tttgttagag
      781 gcactgaacc cttaaaatgg gaacttaata aaaacactat gttctaggct g
//
LOCUS       DALAAR010000212          808 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000212 DALAAR010000000
VERSION     DALAAR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.220"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..>808)
                     /locus_tag="O2458_002921"
     rRNA            complement(<1..>808)
                     /locus_tag="O2458_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          242 c          173 g          223 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgcgga tccatctata
      781 agtgacagca agaccgtctt tcactttt
//
LOCUS       DALAAR010000213          807 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000213 DALAAR010000000
VERSION     DALAAR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.221"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            65..592
                     /gene="isaB"
                     /locus_tag="O2458_002922"
     CDS             65..592
                     /gene="isaB"
                     /locus_tag="O2458_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCZ3888713.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          324 a           87 c          143 g          253 t
ORIGIN      
        1 tggctgaaaa agttataata aaagtgtaag gaataaaata tttgtatgga aaagagagat
       61 aattatgaat aaaaccagta aagtttgtgt agcagcaaca ttagcattgg gcacactgat
      121 tggagtaaca gttgttgaaa attcggcgcc aacaagtaaa caggcacagg cagcaataac
      181 cccatattat acttataatg gttatattgg taataatgct aattttattc tggataagaa
      241 ttttattaac gcgattaagt atgataatgt gaaattcaat ggtattaaat tagctaaaac
      301 gaatacgata aaaaaagtag agaaatatga tcaaactttt aaaggtgttt ctgcaaaagg
      361 aaacgaagca agtcaattgc aatttgtagt taaaaataat atttcattaa aagatatcca
      421 aaaagcttat ggcaaggact tgaaaaaaga aaatggtaaa acaaaggaag ctgatagcgg
      481 tattttttac tatcaaaatg ctaaaaagac attaggcatc tggtttgtcg ttgatcataa
      541 tagagttgtc gaagtaacag ttggacatac accatacaaa acaagtaaat aaaataatgg
      601 catattaagg ctagagtgtg aggagtgata ccgcattcta gtctttttta ttaaataata
      661 acgattattg cgtcttacat agttgtttga aattgataaa aatgtttatt tgattatgat
      721 tatttaaata gttatctcac atatttcaag cattgattaa gtatatagag cactattttg
      781 tatttgttaa tattttcaca aaaataa
//
LOCUS       DALAAR010000214          804 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000214 DALAAR010000000
VERSION     DALAAR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.222"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            3..>804
                     /locus_tag="O2458_002923"
     CDS             3..>804
                     /locus_tag="O2458_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCZ3888714.1"
                     /translation="MNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIFDAMTYAL
                     FGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGNTTKTNAK
                     FDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFLISNSREK
                     QGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDDNIKGLLE
                     VATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENN"
BASE COUNT          335 a           94 c          145 g          230 t
ORIGIN      
        1 agttgaataa tttcggcccc tttttaaaag aagaaattga tttttctaaa attgataata
       61 atgaattgtt tttaataagt ggtaagactg gatcgggtaa aacaatgatt tttgatgcaa
      121 tgacttatgc cttgtttggt aaagcatcaa ctgaacaaag agaagaaaat gatttgagaa
      181 gtcatttcgc tgatggtaaa cagccgatgt cagtaacatt tgaatttcaa ttaaatcatc
      241 gaatttataa agtgcataga caaggccctt atatcaaaga aggtaataca acaaaaacga
      301 acgctaaatt tgatgtattt gagatggtgg atggcaagta tgaaattaga gaaagtaaag
      361 taatttcagg tacccaattc attattgaat tattaggagt aaatgcagat caattccgac
      421 aattgtttat tttgcctcaa ggtgaattca aacgcttttt aatatcaaac agtcgtgaaa
      481 agcaagggat attaagaaca ctgtttgaca gtgaaaaatt tgaagctata cgagaaatat
      541 taaaagaaga agtaaaaaaa gaaaaagctc aaatcgagaa tagatatcaa caaattgacc
      601 ttttatggca agaaattgaa tcatttgatg atgacaatat aaaaggctta ttagaggttg
      661 ccactcaaca gatagacaaa ttgattgaaa atataccact tttacaagct aggtcgaaag
      721 aaatactagc atctgtaaat gaaagtaaag aaactgctat taaagaattt gaaataatag
      781 aaaagaaaac attagaaaat aata
//
LOCUS       DALAAR010000215          777 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000215 DALAAR010000000
VERSION     DALAAR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.223"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..174
                     /locus_tag="O2458_002924"
     CDS             <1..174
                     /locus_tag="O2458_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCZ3888715.1"
                     /translation="KRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKEENIY
                     FSDSLDYKKSGDV"
     gene            226..>777
                     /locus_tag="O2458_002925"
     CDS             226..>777
                     /locus_tag="O2458_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888716.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSA"
BASE COUNT          336 a           75 c          150 g          216 t
ORIGIN      
        1 aaaagatatg atataccgac gagtaaagct ccaaagttat tgttaaaagg ttcagggaat
       61 ttaaaaggct catcagttgg atataaagat attgaattta cgtttgtaga gaaaaaagaa
      121 gaaaatatat actttagtga tagcttagat tataaaaaaa gcggagatgt ataatcatgg
      181 ctcaatcaga atatgaaatc aatcccggaa aaagagagtg atgaaatgat aaaacgtgta
      241 aataaattag tgcttggtat tagtcttctg tttttagtca ttagtatcac tgctggttgt
      301 ggcatgggta aagaagcgga aataaagaaa agttttgaaa aaacattgag tatgtatccg
      361 attaaaaatc tagaggattt atacgataaa gaaggatatc gtgatgatca atttgataaa
      421 aatgataagg gaacatggat tgtaaattct caaatggcaa ttcaaaataa aggagaagct
      481 ctaaaaataa aaggcatgct tttgaagata gatagaaata caagaagtgc aaaaggattt
      541 tactatacta atgaaataaa gacggagaaa tacgaagtag ctcaggataa tcaaaaaaaa
      601 tatccagtta aaatgattaa taataaattc atttctactg aggaagttaa agaagaaaac
      661 ataaaaaaag aaatcgaaaa ctttaagttt tttgcgcaat atagcaattt taaagattta
      721 atgaattata aagatggaga tatatcatat aatccagagg tgccgagtta ttcagct
//
LOCUS       DALAAR010000216          746 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000216 DALAAR010000000
VERSION     DALAAR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.224"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..640)
                     /locus_tag="O2458_002926"
     CDS             complement(<1..640)
                     /locus_tag="O2458_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ3888717.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIMQY"
BASE COUNT          277 a          112 c           94 g          263 t
ORIGIN      
        1 tgtattgcat aataaattga aatgccacaa attgtgtaca agcaataata atgatactta
       61 ccattatgac aaacttggat tctacgacat tgggcaccaa tatatattcg ataattcttt
      121 caatatatat aaatgcgctc aacgcggtta ataataaacc gaaatgtgtt ttagttttac
      181 caccccaacg ttttgttact ttaggtaatt ttaataacgt gaacattccg ccaattacta
      241 ataacaaata actaattatg attgaaatcg tacccatcaa ccaataattg aataggtcat
      301 aatgattttg tacagcaaca aaataataaa ggttacatgt tgcaaatact gcggtgatag
      361 tagttagcac atttataata actaaaaatc gggcatatat tttcttatat ttctgtgcgc
      421 tttccttttc gaaatctata aatctaatcc attgaagtaa actaattatg ataattatta
      481 attctataat tgtcgttatc tttaaataat ttggaaacct ttcataatct aaaccaaaaa
      541 agaaagttag acatgaaaaa agtatcataa ataatgatgc taaacttaag gcttgtgcac
      601 ctggctttcg aagaatgtct atattttcct tagtagtcat atttacctat ctcctataca
      661 ttgattttgt aatagcatat taagtattta aagttataag tacttagatt cttttcgatt
      721 aaaagtataa aagcctaaaa ttatct
//
LOCUS       DALAAR010000217          738 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000217 DALAAR010000000
VERSION     DALAAR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.225"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            246..496
                     /locus_tag="O2458_002927"
                     /pseudo
     CDS             246..496
                     /locus_tag="O2458_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          289 a           94 c           90 g          265 t
ORIGIN      
        1 tttgattaac taattaattt acaataccta aaatgttgtt tggttttgtt tataccaagc
       61 ttcaaactta aatgtcataa caacattcat ttcttaattc cgattagatt tgtcgattat
      121 atttacagca tctttatact caaaaaacat ttacttaaaa atataaattc gatttaataa
      181 ttaatttaaa tttagttaat caattttgca tctatttggt tgtaagctat ataaaaggag
      241 tgataatgat ggtgaaaaaa acaaaatcca attcactaaa aaaagttgca acacttgcat
      301 tagcaaattt attattagtt ggtgcactta ctgacaatag tgccaaagcc gaatctaaga
      361 aagatgatac tgatttgaag ttagttagtc ataacgttta tatgttatcg accgttttgt
      421 atccaaactg gagactttta acataaaatt acttatcatt caaaaagtaa aacagcataa
      481 tatcaaggtt tataacttta tcattatcaa taatacctca tataaaataa aattttaggg
      541 actttttagg gactttaaat ttaaaattac aagtttaata gaaacatcaa aataatcaca
      601 tgtttgtgtg gaatgtacac cccaaaagct agactgaaaa atctattttt tgaggtgtat
      661 ttttataggt aaatataata aattagagta gacaactcag aattccaatt ttataataat
      721 attgcttgac atatcaaa
//
LOCUS       DALAAR010000218          719 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000218 DALAAR010000000
VERSION     DALAAR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.226"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..173
                     /locus_tag="O2458_002928"
     CDS             <1..173
                     /locus_tag="O2458_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCZ3888718.1"
                     /translation="KVSFDYIDWINSEFGQVGRQNYYKYRKFGILPETEYEINKVKEI
                     EQYIKEQDEAEL"
     gene            184..675
                     /locus_tag="O2458_002929"
     CDS             184..675
                     /locus_tag="O2458_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888719.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
BASE COUNT          294 a           62 c          145 g          218 t
ORIGIN      
        1 taaaagtttc atttgattat attgattgga taaattcaga atttggtcaa gtaggtcgac
       61 aaaattacta taagtataga aaatttggaa ttttaccaga aacggaatat gaaattaata
      121 aagttaaaga aatcgagcaa tatattaaag agcaagatga agctgaacta taggggcgat
      181 aatatgactt tcgaagaaaa attaagtgaa atgtatagcg agattgcgaa taagattatt
      241 agcatgatac cggtagagtg ggaaaaagta tatgcaatgg catatatcga tgaagaatgt
      301 ggagaagtgt tttacaatta tactgaacca agcagtgatg aattatttta ctatacaagt
      361 gtgataaaga agtataattt attgaaatca agctttatgg attcagtata tgagttgcat
      421 gatcaatttg aggaactaag agaagtattt atagaagaag gtcttgaacc atggacatca
      481 tgcgaatttg attttacaaa agaagataaa ttaaaagttt catttgatta tattgattgg
      541 ataaatacag agtttgatca attaggccgt gaaaattatt atatgtacaa aaaatttggt
      601 gttttaccag aaacggaata tgaaatggag gaggttaaag aaatcgagca atatattaaa
      661 gagcaagaag aatagacaat cttaatgtaa gattaaacaa taaagctttg tttagtctt
//
LOCUS       DALAAR010000219          711 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000219 DALAAR010000000
VERSION     DALAAR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.227"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..145)
                     /gene="msa"
                     /locus_tag="O2458_002930"
     CDS             complement(<1..145)
                     /gene="msa"
                     /locus_tag="O2458_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCZ3888720.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFK"
     gene            complement(342..542)
                     /gene="cspA"
                     /locus_tag="O2458_002931"
     CDS             complement(342..542)
                     /gene="cspA"
                     /locus_tag="O2458_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCZ3888721.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          262 a          125 c           84 g          240 t
ORIGIN      
        1 ttttgaaaaa tagtatccca gaaatcatta tcggaatcac taatacaatc atcattgctg
       61 ctaaaatatt aatatttaat ccaattgcag ataatacacc gaaaactaat aaattagcaa
      121 ctaaactaag gataagatat ttcataaaat cacctcgtcg ttgtacacca taattatatt
      181 atataaatat atttttctat tgcaaacaat gttggtgata taaaaaaaga gttctcaatt
      241 tggttgagaa actcatcgcg ttaagtattt gtaataagca tataaaaaca ccttacttct
      301 tggtaaggtg ttagttaaaa tcaatgattc aaatctaaga attatagttt aacaacgttt
      361 gcagcttgtg gaccgcggtc gccttcaact acttcaaact caacagcttg accttcttct
      421 aatgatttgt aaccatcttg gttaattgct gaaaaatgta cgaatacgtc attttctcct
      481 tcaacttcga taaagccgaa tcctttttca gcgttaaacc atttaactgt accttgtttc
      541 ataatctgaa acctccaaga ctaaaattca ttcaatatgc ttattcgcat attacaaaaa
      601 atacatgcaa atttcactca aatattcttt gcaatatgga ataatcatct gcttaacttg
      661 tattatagtg cagttattac caaattgcaa tacaataata aaaaatattc a
//
LOCUS       DALAAR010000220          685 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000220 DALAAR010000000
VERSION     DALAAR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.228"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..>685
                     /locus_tag="O2458_002932"
     CDS             <1..>685
                     /locus_tag="O2458_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ3888722.1"
                     /translation="KDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSLENNKPTV
                     MLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKVSLSKDLV
                     DRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSNFNSIEEA
                     IVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTAYNLLEKY
                     KETEIDIDNI"
BASE COUNT          291 a           74 c          106 g          214 t
ORIGIN      
        1 aaaagatgat gtaataaatt tttataaaga gcagatggct ggttctaaaa taataggtca
       61 aacagacgct aaaaaacaaa tgagcataga tttatataaa catacttcct tagaaaataa
      121 taaaccgacc gtaatgcttt actatggtcc atcaggtgtt ggaaaaacag aattagccaa
      181 agaaatagca aaaaaatata aaggtaatat cactagaata caattttcta tgatgcagaa
      241 tgatgaatct tttaattata tctttggaga tgaacatgcc aaagtatcat tgtcaaaaga
      301 tttagtagat cgtgaaacta atgttgtttt aattgatgaa tttgataagg tattaccaat
      361 ctattacaat gttttctatc aaatgtttga tgaaggtatt attgaagatg ccaattataa
      421 agtagacgtt tcaaattgta ttttcattct aacatctaat tttaatagta ttgaagaagc
      481 aatagttaat attggagaac cggtattttc tagaatagat tcctgtatta aatttttata
      541 tttaacacag gaagaaaagc agaaagtaat agaaaataaa ttaaatgaaa tactatcatc
      601 tctaaatgaa aaagaaaaaa gaatagttac cgcatataac ttgttagaga aatataaaga
      661 gacagaaata gatatcgata acatt
//
LOCUS       DALAAR010000221          646 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000221 DALAAR010000000
VERSION     DALAAR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.229"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          278 a           75 c           89 g          204 t
ORIGIN      
        1 ctattggatg gaaaatatat ctagaaaaaa tttctcttcg agatagacaa gaatggtggt
       61 acaatgtttt ttatgacgat ggcaaatata ataagaaaat aattaagaat gacatgtcaa
      121 aaattaggcg taatttttag atttatctat atatttatgc caccgctcaa acttttataa
      181 aaagtaaaaa aacaatattt atattgaact tgtgacgtga atttctaaaa cacaaccaac
      241 aatccacaag gaaactagta aataagttta aataagattt cgaaaaaatg tgtaatatta
      301 taatatgaaa atctttaaga ttatagaaga atcatactga ctaaaaagat tttgaaagtg
      361 attaagtatt taaaacacgc caatcttaaa ccgctattga caaatatgaa gcggaaccac
      421 cgttgttttt cttttcaggt ccaattgaat aaatgttatg aatagaatgg ataaaaatat
      481 aattatttaa gtgttaaagt aaaccaaatc atgatttaag attataagaa agaaaaattg
      541 actaatgtta aaaccaacaa tcatatgggt tgtaacgttt atacaatcat cgtaaaactc
      601 aatatgctta atttattcaa aatgttcgtc aataagtaca atgaaa
//
LOCUS       DALAAR010000222          594 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000222 DALAAR010000000
VERSION     DALAAR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.230"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..174
                     /locus_tag="O2458_002933"
     CDS             <1..174
                     /locus_tag="O2458_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCZ3888723.1"
                     /translation="KRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEFTFLENKNENIY
                     FTDSLHLEPSEDK"
     gene            238..>594
                     /locus_tag="O2458_002934"
     CDS             238..>594
                     /locus_tag="O2458_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888724.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRP"
BASE COUNT          245 a           63 c          119 g          167 t
ORIGIN      
        1 aaaagatatg acataccaac aaataaagcg ccgaagctgt tgttgaaagg tacagggaat
       61 ttaaaaggtt catcagttgg atataaaaaa attgaattta cttttttaga gaataaaaat
      121 gaaaatattt actttactga tagtctacat cttgaaccga gcgaggataa ataatcatct
      181 tttaaccaga atatgaaatc aatctaggac aacctaataa aaggagagag ttgtaagatg
      241 atgaaacgat taaacaaatt agtgttaggc attatttttc tgtttttagt cattagtatc
      301 actgctggtt gtggcatagg taaagaagcg aaaataaaga aaagttttga gaagacattg
      361 agtatgtatc cgattaaaaa tctagaggat ttatacgata aagaaggtta tcgtgatgac
      421 gaatttgata aaaatgataa aggtacatgg attattggtt ctgaaatggc aactcaaaat
      481 aagggggaag ctctgaaagt taaaggtatg gtcttgtata tgaatagaaa taccaaaaca
      541 acaaaaggat attattatgt aaatgcaata aagaatgata aagacggaag accc
//
LOCUS       DALAAR010000223          588 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000223 DALAAR010000000
VERSION     DALAAR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.231"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            154..360
                     /locus_tag="O2458_002935"
     CDS             154..360
                     /locus_tag="O2458_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888725.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          254 a           63 c           84 g          187 t
ORIGIN      
        1 aattttatca atatataaaa aaaacgccaa tactattagg tattagttta tgaattaact
       61 agtttggcaa ctagaaaaaa acaatgtatt gctaaacgta gttactgcat taggattatt
      121 ataattataa atagaaagag agtgtgatta ataatgaatg agattgaaac tattataagt
      181 gaaatagaaa agttattaac taacaataca ccatatagta tttcaaaaaa ctcaggtgta
      241 ccacgtcaaa cagttactga tttaaaggta ggtaaaacta aaataaaaga agctaaattt
      301 aaaacgataa tcaagttata tgaatatcaa agaacattag aaaataaaac agaatgttaa
      361 caaaaatatt ttgttaggtt aggtggacaa gaaattaaat ttctaatgat ttcatggttg
      421 tctgtccacg ttttgtccac ctcaataaga taatctctta gttttagaaa ttgtacatgg
      481 ttaaataatc aaaaatgcat attatgaaat tatcattgcc attaaatctt gaatgatctt
      541 taagcgatga tataaaaggc tgaacacagt aaataagtta actttaat
//
LOCUS       DALAAR010000224          545 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000224 DALAAR010000000
VERSION     DALAAR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.232"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..412)
                     /locus_tag="O2458_002936"
     CDS             complement(<1..412)
                     /locus_tag="O2458_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ3888726.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNN"
BASE COUNT          193 a           86 c           54 g          212 t
ORIGIN      
        1 cattattttt actttcatta taaatattta atgttaattt cttatttaga taacaataaa
       61 aaaatataat ctctaaaaac ccaatgaaac agctgctcca aacaattagt tcttctaatt
      121 gaaaatcaaa taaataagta tttcttctta cagtaccacc aagtaacaca atacccgcga
      181 atatggtcca tcctatcgat ttatttttaa ctggtttgac aatatttaat ttttcataat
      241 cattcttagt gattttgaca tatgttttcg gtatgagcca gttaataaat ggaaagaaga
      301 agacaatcca attacttgcc aaatcaatca ttaaatattc actatcgtat ttgattattc
      361 gatatttagg gtttttatta ataactttag attcgcaaag caatgtctcc acatcccttt
      421 aattttatgt gtaatacatt tttcgatact tcaaaagaca ttcaaatact atcaagttac
      481 tgtcatcaaa ggttttatta actgatattt tcatattttt aatctgaatt tagtactttc
      541 actta
//
LOCUS       DALAAR010000225          544 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000225 DALAAR010000000
VERSION     DALAAR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.233"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            123..>544
                     /locus_tag="O2458_002937"
     CDS             123..>544
                     /locus_tag="O2458_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ3888727.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANAN"
BASE COUNT          200 a           88 c           74 g          182 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 atag
//
LOCUS       DALAAR010000226          537 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000226 DALAAR010000000
VERSION     DALAAR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.234"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          220 a           83 c           67 g          167 t
ORIGIN      
        1 aaatgttgga tgtgaaagat tgttgatgag aattttgaga tgtagataaa tatcaaacta
       61 gtctataggt aaaaatttat aattaatttg aaaagaatgt ctcaaataat gtttgaatta
      121 gcaggtgtca tactaagtaa tgttaagtaa acattttata ctttatcaga acaaaaaaga
      181 catgcatcaa catgtcagtg aaactaatcc atattatctt aacaacgaaa ttgaatcgaa
      241 cctgaaaaac gtatttacaa atcgcaacta ctctatccaa ggcaaaatcg caaaatctga
      301 tccccgaaat aacatatctc gaaaaaaatt ccataattca atcatcttca atatttaaca
      361 atacattact agttccaaat atactcatac atacatgttt cattgattga ctgcatattg
      421 tcataaataa taggggaact aatcgctaat aatgttgtta aacacaatac atagtaaagt
      481 gaaagattat aaatacatga attgttcacc attccgccat atattttcaa tcaccta
//
LOCUS       DALAAR010000227          530 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000227 DALAAR010000000
VERSION     DALAAR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.235"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..351
                     /locus_tag="O2458_002938"
     CDS             <1..351
                     /locus_tag="O2458_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888728.1"
                     /translation="IKDEKIKKEIENFKFFVQYGNFKDLSKYKDGDISYNPEVPSYSA
                     KYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKDIEFTFVEKKEENI
                     YFSDGLIFKPSEDK"
     gene            415..>530
                     /locus_tag="O2458_002939"
     CDS             415..>530
                     /locus_tag="O2458_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ3888729.1"
                     /translation="MMKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFE"
BASE COUNT          220 a           51 c          103 g          156 t
ORIGIN      
        1 attaaagatg aaaaaataaa aaaagaaatc gaaaacttta agttctttgt tcaatatgga
       61 aactttaaag atttgtcgaa gtataaagac ggagatattt catataatcc agaggtgccg
      121 agttattcgg ctaaatatca attaactaat gatgattaca atgtaaagca attacgtaaa
      181 agatatgaca taccaacaaa taaagcaccg aagttgttgt tgaagggtac agggaattta
      241 aaaggttcat cagttggata taaagacatt gaatttacgt ttgtagagaa aaaagaagag
      301 aacatttact ttagtgatgg gttaattttt aaaccaagtg aggataaata atcatgactc
      361 gatcaaaata tgaaatcaat ccaggaaaga taataagaag aataggattg tgaaattatg
      421 aaacgattaa acaaattggt attgtatatt agttttttga tattagtcat tagtttcact
      481 gctggttgtg gcataggtaa agaagcggaa gttaagaaaa gctttgaaaa
//
LOCUS       DALAAR010000228          511 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000228 DALAAR010000000
VERSION     DALAAR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.236"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(30..422)
                     /locus_tag="O2458_002940"
     CDS             complement(30..422)
                     /locus_tag="O2458_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888730.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          172 a           93 c           40 g          206 t
ORIGIN      
        1 cctcttactc taattatatt cactatcaat tagacaaaat ggccattttc aaatatcacg
       61 cgttgtttct gaccttgaat atatttatta taattctctt tttgaaaatc agttaacatt
      121 aatttagatg taccgtattt taacactttt tgcattgttt ctattctcat ttttctaaat
      181 aaccatccat cttttaacac aatacgatta acagcatcat atgataattc tactgtttct
      241 ttaatttcaa atgtcttgaa tgaaataatc gtgcacatta aaaacgtatc accaaagtaa
      301 taaacatcta aatcatcacg tttatgttgt ccaactaaca aacgaccata ttcgaactct
      361 ttttctggat atttcaattc taaaaaacta ataatctctt cttcttttaa tttgaattgc
      421 atttaaaaac atcctctctt aagttttaac aagccttaat taaaaatttt ttcaatcaca
      481 tagttcaata tacatcattt cgttatgttt t
//
LOCUS       DALAAR010000229          507 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000229 DALAAR010000000
VERSION     DALAAR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.237"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            1..507
                     /locus_tag="O2458_002941"
                     /pseudo
     CDS             1..507
                     /locus_tag="O2458_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a           71 c           84 g          161 t
ORIGIN      
        1 tcctcaaaat gatatttcgc gatatgttaa tgaaattgtt gaaacgatac ctgatagcga
       61 attcgatgaa ttcagacatc atcgtggtgc aacatcctat catccaaaaa tgatgttaaa
      121 aatcatctta tatgcatata ctcaatctgt attttctggt cgtagaatag agaaattact
      181 tcatgacagt attcgaatga tgtggttagc ccaagatcaa acactttctt ataaaactat
      241 taatcgtttt agagtgaatc ctaatactga tgcgttaatt gaatctttat ttattcagtt
      301 ctatagtcaa tgtttaaagc aaaatcttat tgatgataat tcaattttta ttgatggtac
      361 aaaagtagaa gctagtgcca atagatatac attggtgcgg aagaaagtat tcaaaatcac
      421 gaatcgaaat tgaacgagaa ttcaaaaaca ctatatcgtg acttagttga agaaaaaata
      481 ataccagaga taaaagaaga tggagat
//
LOCUS       DALAAR010000230          478 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000230 DALAAR010000000
VERSION     DALAAR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.239"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            243..478
                     /locus_tag="O2458_002942"
                     /pseudo
     CDS             243..478
                     /locus_tag="O2458_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          193 a           72 c           66 g          147 t
ORIGIN      
        1 aaataagaca ctttgcccaa cttgcacata aatgtaaaat tcaataaaat aaattttctg
       61 tgttggatcc cttcgtataa tttaataaat actactaaac taaattaacg aggtgcctta
      121 tgtataaaaa ttataacatg acccaactta cactaccaat agaaacttct gttagaattc
      181 ctcaaaatga taatacgcga tatgttaatg aaattgttga aacgatatct gatagcgaat
      241 tcgatgaatt tatatgtcct aataacaaaa gaataggttt taaaagatat gcataccgta
      301 atgatagata tagttttaaa cgtgacttca agctatatga atgtgatgac tgttcatcat
      361 gttctttgag acatcaatgc atgaagccaa attcgaaatc caataagaaa attatgaaga
      421 attataattg ggaatacttt aaagcccaaa ttaatcaaaa gctttctgaa ccagaaac
//
LOCUS       DALAAR010000231          450 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000231 DALAAR010000000
VERSION     DALAAR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.240"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            8..>450
                     /locus_tag="O2458_002943"
     CDS             8..>450
                     /locus_tag="O2458_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCZ3888731.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPG
                     FAQSISYYIDQLQMKEILGIIGGNDTLMILTSSNEIAEFVCYQLFP"
BASE COUNT          195 a           54 c           58 g          143 t
ORIGIN      
        1 aataaaaatg aaaaaagaaa aacgtttaaa tcttatctta actgttatac aacagaatca
       61 gtttaataaa aaacaacaaa tagtggatta catggcaaga cattttggag tttactatag
      121 cttgacaact atttctcgtg acttacaaga attagaaatt tacaaaatcc ctgttgaaaa
      181 taaaaagtat atttacaaga aaataaatca aacaaatcaa ttaagtgcaa aaaaacaatt
      241 agaaatattt agtgatgaga ttattgaatt tataacgcta aataactatg tcttaataaa
      301 aacatctcct ggctttgctc aaagtataag ttattacata gatcaattac aaatgaaaga
      361 aatattagga attattggag gtaacgatac tttgatgatt ttgacttctt caaatgaaat
      421 agcagaattt gtttgttatc aattattccc
//
LOCUS       DALAAR010000232          450 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000232 DALAAR010000000
VERSION     DALAAR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.241"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..137
                     /locus_tag="O2458_002944"
     CDS             <1..137
                     /locus_tag="O2458_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888732.1"
                     /translation="KAQAFDEIREAINAQWVEYPEDWASEVLREVRELEYEEEQENEN
                     "
     gene            127..369
                     /locus_tag="O2458_002945"
     CDS             127..369
                     /locus_tag="O2458_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888733.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            362..>450
                     /locus_tag="O2458_002946"
     CDS             362..>450
                     /locus_tag="O2458_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCZ3888734.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDI"
BASE COUNT          185 a           51 c          106 g          108 t
ORIGIN      
        1 agaaagcgca agcgtttgat gaaattcgcg aagctattaa tgcgcaatgg gttgagtatc
       61 cagaagactg ggcgtcagag gttttgagag aagtaagaga gcttgaatat gaggaggaac
      121 aggaaaatga aaattaatga atctttgaag aaattaaaag aaaaaggata caaagaaaat
      181 gaagataaag ctatttttaa tttagctgat ggtacgctag aaatttacat cgaccatgac
      241 gaaaaaacaa taattacaga attccatgat ttaaaggtat tcgtgtcaga agatttaaaa
      301 gataaaagta tggagagcgt tatgtatgaa ttggctggaa ttgatgagga ggacaaggaa
      361 aatgactaac atcctacaag taaagctatt atcagaaaac gctagaatgc cagaacgaaa
      421 tcataagacg gatgcaggtt atgacatatt
//
LOCUS       DALAAR010000233          448 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000233 DALAAR010000000
VERSION     DALAAR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.242"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          185 a           47 c           77 g          139 t
ORIGIN      
        1 tttgagtata tatggagttc tttcttttta tacaagatgt atatgtataa atggaaatat
       61 ttaatacatt acataaaaac ctttaggtgc acttagaggg atgttaatca taaaaaaaga
      121 gattttgaaa aatataggct attatacttt agaattacaa attataaatc ttgccagtgg
      181 atatgggagt tcaacatgtc acttacccca atttgtggaa tgttaaataa ctataaagag
      241 gaaaggagaa aaaatgataa gaatggttaa agaaaaagaa cgaatagcaa aattaaattt
      301 cgaatagctg tgacgagctt aataattcaa gtacttaatt ttatcaaatc gttcttttaa
      361 aaaggtaaag ggcaaaagac ctttaccaca ttaaataggg aggtggtatt tatgacaaga
      421 gaattaagga aaaaattaac tctctatc
//
LOCUS       DALAAR010000234          446 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000234 DALAAR010000000
VERSION     DALAAR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.243"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..414)
                     /gene="sarU"
                     /locus_tag="O2458_002947"
     CDS             complement(<1..414)
                     /gene="sarU"
                     /locus_tag="O2458_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCZ3888735.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFF"
BASE COUNT          135 a           69 c           53 g          189 t
ORIGIN      
        1 aaaaaattct ttgctatatt ttggagccca ttgatatttt atgtagtctc tttccttatt
       61 aaatccttta attattttat ctaactcatt aataacacaa gcaatctttt tacgttgtat
      121 tggagtaact gaaacaaaaa tacggcgttc atctgcttcg tttctagact tatttataaa
      181 tcctttttta gatagtgact taatgttctt tacaacatca gattgtttgt aaagtatgtc
      241 accaatgatt tcttttagtg aaatactgtg ttcatttttg taataaaaat atgctaatat
      301 caacaattct tttaatgata atttatattg ttgctttaac tcttgagtga gaaaaaatat
      361 gtacgcttct acatttatga atttattgac cttttcgaaa gtttgatagt ccaccttaca
      421 ttctcctact attttctgat gtccca
//
LOCUS       DALAAR010000235          442 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000235 DALAAR010000000
VERSION     DALAAR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.244"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            95..229
                     /locus_tag="O2458_002948"
     CDS             95..229
                     /locus_tag="O2458_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCZ3888736.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT          169 a           52 c           69 g          152 t
ORIGIN      
        1 aataaaaaac gactagttaa gaaaaattgg aaaataaatg cttttagcat gttttaatat
       61 aactagatca cagagatgtg atggaaaata gttgatgagt tgtttaattt taagaatttt
      121 tatcttaatt aaggaaggag tgatttcaat ggcacaagat atcatttcaa caatcggtga
      181 cttagtaaaa tggattatcg acacagtgaa caaattcact aaaaaataag atgaataatt
      241 aattactttc attgtaaatt tgttatcttc gtatagtact aaaagtatga gttattaagc
      301 catcccaact taataaccat gtaaaattag caagtgagta acatttgcta gtagagttag
      361 tttccttgga ctcagtgcta tgtatttttc ttaattatca ttacagataa ttatttctag
      421 catgtaagct atcgtaaaca ac
//
LOCUS       DALAAR010000236          442 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000236 DALAAR010000000
VERSION     DALAAR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.245"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(90..>442)
                     /gene="fmhC"
                     /locus_tag="O2458_002949"
     CDS             complement(90..>442)
                     /gene="fmhC"
                     /locus_tag="O2458_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ3888737.1"
                     /translation="TEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAY
                     HLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPV
                     RPILYKFAKLIYKV"
BASE COUNT          162 a           69 c           52 g          159 t
ORIGIN      
        1 tgttatttta aggagtaaaa atactgactt aaaaagtgaa aagtatgttg gaaagaattt
       61 aaaatttcaa tttccaacat actttataat taaaccttat aaataagttt tgcaaattta
      121 tatagaattg gtcttactgg tttgatgaaa tcaccaatta attcttcaac atgtgcatta
      181 aaaccctttt taaattgttg aacaccaaaa tcatccgcct cattactaaa gacgcctgtt
      241 attccataaa aattatacct attaatatta tgtgattttg catattttat catatgccat
      301 tgtagatgat atgcacccat atactgatta tatttcggat ttgatccact tgatagataa
      361 taaacttcat catcagtaca tataaataaa gcagcagcta aatccaaaac aggtccatct
      421 tcaaatatta gttcttcggt tt
//
LOCUS       DALAAR010000237          431 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000237 DALAAR010000000
VERSION     DALAAR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.247"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          115 a           96 c           55 g          165 t
ORIGIN      
        1 ttcgatttaa aagatattag acataaaatc taaaaacagc agtaagatga tttatgatta
       61 aaaactatct tactgctgtt cactttttat aatacttctg aatgtcttca cttatacttc
      121 tagtcacaga tttaaataat caaaagtgca cattattaaa atatcaattt cacactcaat
      181 gcggctcatc gcattcattt cttgtctagc aacgttctac tctagcggaa cgtaagttag
      241 ctaccatcct cgctaagaac ctttcttgac ttgtgacaat cgcttgcttc tttcctctcc
      301 ttcggctctc gcttactcat ttagctctac taaactcgtt gcgctctttt ctcgtttcgt
      361 cagattcaaa cgttttcact tcgccaagcc atttttcttt gtgtttgctt tttattttga
      421 cgttttagac a
//
LOCUS       DALAAR010000238          416 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000238 DALAAR010000000
VERSION     DALAAR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.248"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            184..266
                     /locus_tag="O2458_002950"
     tRNA            184..266
                     /locus_tag="O2458_002950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:217..219,aa:Leu,seq:gag)
     gene            289..362
                     /locus_tag="O2458_002951"
     tRNA            289..362
                     /locus_tag="O2458_002951"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:321..323,aa:Gly,seq:tcc)
BASE COUNT          146 a           66 c           77 g          127 t
ORIGIN      
        1 taatttataa aatatgttga caataagttc taaatgtttt attataaata gcgttgtgtt
       61 aacaactgat aacacaaatt aatttaatgt aaaaaaacga ttaaaagtta ttgacaaata
      121 tttttaaaca taatataata aataacgttc tgaaaaacga agatgtttta aatgcctata
      181 tccgcggtcg tggcggaatg gcagacgcgc taggttgagg gcctagtggg agaaatcccg
      241 tggaggttca agtcctctcg gccgcatcaa aattcttaat ttaaataagc gggtgtagtt
      301 taatggcaaa acctcagcct tccaagctga tgttgtgggt tcgattccca tcacccgctc
      361 catagataat tttaatgaac attgaaaact gaatgacaat atgtcaacgt taattc
//
LOCUS       DALAAR010000239          405 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000239 DALAAR010000000
VERSION     DALAAR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.249"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          162 a           53 c           50 g          140 t
ORIGIN      
        1 taatgataag aaaaacacat taatttagtg attaaaacca atctatagca cgttaaagtg
       61 tctatacgtt attccgattg ataacgggta acaaataata gaaagtacga taattgatca
      121 tgcattaaag cacttttatt tttcaaactc gactcaatga attataaaaa gttgtgcatt
      181 ttgacttatt tttaattaat tgaataacca actatgaaat attagcacga ataacataat
      241 ttttgctatt aatttggtgt aaattattaa aaaataacat taatttaggt cttaagaatg
      301 agttgactat cgaatttgct aaattaaaat ttgttgtgtc aaagcatact acccaaatca
      361 acgcttttct aaagattttt agaccacaaa aaaatagctt atcac
//
LOCUS       DALAAR010000240          402 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000240 DALAAR010000000
VERSION     DALAAR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.250"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(103..>402)
                     /locus_tag="O2458_002952"
     rRNA            complement(103..>402)
                     /locus_tag="O2458_002952"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          103 a          102 c           85 g          112 t
ORIGIN      
        1 gctataggcc cattaatttg aatgaacaaa cattcaaaac tgaatacaat atgtcacgtt
       61 attccgcatc ttctgaagaa gatgttccga atatatcctt agaaaggagg tgatccagcc
      121 gcaccttccg atacggctac cttgttacga cttcacccca atcatttgtc ccaccttcga
      181 cggctagctc ctaaaaggtt actccaccgg cttcgggtgt tacaaactct cgtggtgtga
      241 cgggcggtgt gtacaagacc cgggaacgta ttcaccgtag catgctgatc tacgattact
      301 agcgattcca gcttcatgta gtcgagttgc agactacaat ccgaactgag aacaacttta
      361 tgggatttgc ttgacctcac ggtttcgctg ccctttgtat tg
//
LOCUS       DALAAR010000241          384 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000241 DALAAR010000000
VERSION     DALAAR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.251"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            128..232
                     /locus_tag="O2458_002953"
     CDS             128..232
                     /locus_tag="O2458_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888738.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(223..295)
                     /locus_tag="O2458_002954"
     tRNA            complement(223..295)
                     /locus_tag="O2458_002954"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Lys,seq:ttt)
     gene            complement(299..373)
                     /locus_tag="O2458_002955"
     tRNA            complement(299..373)
                     /locus_tag="O2458_002955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(339..341),aa:Gln,seq:ttg)
BASE COUNT          138 a           76 c           75 g           95 t
ORIGIN      
        1 tggttacaga aatttcaaca actttaaagc acgtataatg atgattttca gcttgtacaa
       61 aggagaaaaa aagaagacaa ccaagcccaa taatggactg gccgcctaat aataaaagct
      121 ctaaaagttg tattttaaaa atagttcttt aaattatata cccaccacat ttggtggaga
      181 acctaaaaaa aagcacttcc caaaaatgga aagtgcaagt agtgagccat agaggattcg
      241 aacctctgac cctctgatta aaagtcagat gctctaccaa ctgagctaat ggctctaatg
      301 gctgggctag ctggattcga accaacgagt gacggagtca aagtccgttg ccttaccgct
      361 tggctatagc ccaatatata gatg
//
LOCUS       DALAAR010000242          371 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000242 DALAAR010000000
VERSION     DALAAR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.252"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..192
                     /locus_tag="O2458_002956"
     CDS             <1..192
                     /locus_tag="O2458_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCZ3888739.1"
                     /translation="FTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYE
                     MEEVKQIEQYIKEQEEAEQ"
     gene            203..371
                     /locus_tag="O2458_002957"
                     /pseudo
     CDS             203..371
                     /locus_tag="O2458_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
BASE COUNT          165 a           37 c           72 g           97 t
ORIGIN      
        1 tttacaaaag aagataaatt aaaagtttca tttgattata ttgattggaa gaatacagaa
       61 tttgatcaat taggccaaga aaactactat aactatagaa aattcggaat tataccagaa
      121 atggaatatg aaatggaaga ggttaaacaa atcgagcaat atattaaaga gcaagaagaa
      181 gctgaacaat agaggcgata acatgatttt cgaagaaaaa ctaaatgaaa tgtacaacga
      241 gattgcgaat aaaattagta gcatgatacc agtagaatgg gaaaaggtat atacaatggc
      301 ttatatagat gatggaggag gtgaagtatt ctttaattat actaaacagc gatgaattga
      361 attattacac c
//
LOCUS       DALAAR010000243          360 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000243 DALAAR010000000
VERSION     DALAAR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.253"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          129 a           63 c           63 g          105 t
ORIGIN      
        1 cgaaaatatc atttctgtcc cactccctaa taactgagtt ttaataaagt cttttaaccc
       61 acattaaata ttattttgca attgcaatga ataacaagaa aaatctggga cattaatcga
      121 tcaaatgctc ccttcaaagt agacattgaa taaatgaagg ctttgaaggg agcatttcac
      181 tttgtacttg gctcaacaat tttatataga cagtagttaa ttgaatgaaa ataagcttgt
      241 aacaagtttt caattctagt cagggcccca acaaagaagc tgacgaaaag tcagcttaca
      301 ataatgtgca agttggggat gggccccaac atagagaaat tgggtccgta atttctacag
//
LOCUS       DALAAR010000244          357 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000244 DALAAR010000000
VERSION     DALAAR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.254"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            50..>357
                     /locus_tag="O2458_002958"
     CDS             50..>357
                     /locus_tag="O2458_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ3888740.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     "
BASE COUNT          145 a           31 c           77 g          104 t
ORIGIN      
        1 gagcaatatg ttaaagagca agaagaagct gaacaataga ggcgataaca tgaatttcga
       61 agaaaaatta agtgaaatgt acaataaaat tgcaaatgag atcaatgaaa tgataccagt
      121 agagtgggaa aaggtatatg taattgcata tgtggacgat ggaggtgggc aagttatatt
      181 ttattatacc aagcccagaa atgatgagct gtattattat tcgagtatcg ttgaagatta
      241 taatgtttta gaagaaatct ttgatgattt atggatggaa ctttatagat catttaaaaa
      301 attaagaaat atatttaaag aagaaagtct tgaaccatgg acatcatgtg aatttga
//
LOCUS       DALAAR010000245          344 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000245 DALAAR010000000
VERSION     DALAAR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.255"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            27..224
                     /locus_tag="O2458_002959"
     CDS             27..224
                     /locus_tag="O2458_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888741.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT          142 a           36 c           61 g          105 t
ORIGIN      
        1 attaaaaatt tcgcctcatc taatttatga attgctatga tgaaatattc aatacaatca
       61 aagaattgat agaaaacaaa gagatatcga gttatcaaat taataaagat actgggataa
      121 gttacggtaa tattaatgct atgcgccgta gagaaagaag aatagaaaat ttaagcttaa
      181 agaatgcaaa gatcttatat gaatatgcga aaaaggtatt gtaatataag cattgagttc
      241 aataggcata caataactgc ccaaactagt gactgagtgg gtgcttttat agtattaaaa
      301 gttttctaaa agtgaaagaa atcatgttta gtgatttact tatg
//
LOCUS       DALAAR010000246          323 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000246 DALAAR010000000
VERSION     DALAAR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.256"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(<1..>323)
                     /locus_tag="O2458_002960"
     CDS             complement(<1..>323)
                     /locus_tag="O2458_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCZ3888742.1"
                     /translation="IVAYKIDDKQDQSLVNDTLNQIDIPEGCILHSDQGSVYTSYAYY
                     QLCEEKGIIRSMSRKGTPADNAPIESFHSSLKSETFYINNELNHSNHIVIDIVEKYIK
                     NNNNN"
BASE COUNT           94 a           52 c           52 g          125 t
ORIGIN      
        1 tattattatt attgttttta atgtactttt cgacaatatc tattacaatg tgattagagt
       61 gattaagctc attattgatg taaaaagttt cagactttag cgaggaatgg aaactttcta
      121 tcggggcgtt atcggcaggt gttccctttc gggacatact tctgataatg cctttttctt
      181 cgcacaattg ataataagca taagatgtat aaacgctgcc ttgatcacta tgtaatatac
      241 aaccctcagg tatatcgatt tgatttaatg tatcattaac taaactttgg tcttgtttat
      301 catctatttt atacgccaca att
//
LOCUS       DALAAR010000247          315 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000247 DALAAR010000000
VERSION     DALAAR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.257"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(171..308)
                     /locus_tag="O2458_002961"
     CDS             complement(171..308)
                     /locus_tag="O2458_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888743.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
BASE COUNT           97 a           54 c           28 g          136 t
ORIGIN      
        1 aatgacataa tttagatgtt ttactatcta ttttggcaac atatttatat tcgccatctt
       61 caccaagttc ttttaaatat gttaactttt gtgcttctgc attttcagta aatagttaaa
      121 aaagcgtata aaaatagcac cactttctct ttatctgtct aaaaaggatg ctatttatct
      181 tttgaatttg aattcttttt cgctttttct atactttcaa attcttcaac taattcttta
      241 aaagattcac tcaattcttt tgcagttaca tttccatcta attgtgaatc taacataatt
      301 aaaatcatct cactt
//
LOCUS       DALAAR010000248          274 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000248 DALAAR010000000
VERSION     DALAAR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.259"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            77..256
                     /locus_tag="O2458_002962"
     CDS             77..256
                     /locus_tag="O2458_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888744.1"
                     /translation="MRKYHFCPTPQKRFYHCQKNASLHVRVNIGQPTKIINTRRCYLM
                     SSDTNSLAHTKWNCK"
BASE COUNT           97 a           55 c           51 g           71 t
ORIGIN      
        1 ggggccccaa caaagagaaa ttggattccc aatttctaca gacaatgcaa gttggggtgg
       61 gacggcgaaa taaattttgc gaaaatatca tttctgtccc actccccaga aaaggttcta
      121 ccattgtcaa aaaaatgcat ctctacatgt tagagtaaat attggtcagc caaccaaaat
      181 aatcaacacg aggagatgct atttaatgtc atctgacaca aacagtttag cacatacgaa
      241 atggaattgt aagtaacata ttgtctttgc acct
//
LOCUS       DALAAR010000249          273 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000249 DALAAR010000000
VERSION     DALAAR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.260"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            10..82
                     /locus_tag="O2458_002963"
     tRNA            10..82
                     /locus_tag="O2458_002963"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Phe,seq:gaa)
     gene            96..171
                     /locus_tag="O2458_002964"
     tRNA            96..171
                     /locus_tag="O2458_002964"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:His,seq:gtg)
     gene            187..260
                     /locus_tag="O2458_002965"
     tRNA            187..260
                     /locus_tag="O2458_002965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Gly,seq:tcc)
BASE COUNT           58 a           65 c           64 g           86 t
ORIGIN      
        1 taccattacg gttcagtagc tcagttggta gagcaatgga ttgaagctcc atgtgtcggc
       61 agttcgactc tgtcctgaac cattcttaat tcatggcggt tgtggtgaag tggttaacac
      121 atcggattgt ggttccgaca ttcgagggtt cgatcccctt cagccgcccc ataatcgttt
      181 acattagcgg gtgtagttta atggcaaaac ctcagccttc caagctgatg ttgtgggttc
      241 gattcccatc acccgctcca ttattttcta tta
//
LOCUS       DALAAR010000250          263 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000250 DALAAR010000000
VERSION     DALAAR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.261"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT          101 a           34 c           33 g           95 t
ORIGIN      
        1 ggtataaata gtagaagaag ttaacttgga agagttaagc tataacaaag aatctcttta
       61 gacacacatt gaatatcgaa acatttaatt gcgctaaatc gtttcattaa ataaattacc
      121 ttgtattgtc gattaaatta aggtaaatta taaaaaatgc tgatattttt gactaaacca
      181 aatgctaacc cagaaataca atcactgtgt ctaatgaata atttgtttta taaacacttt
      241 tttgtttact tctcattttt aat
//
LOCUS       DALAAR010000251          261 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000251 DALAAR010000000
VERSION     DALAAR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.262"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            6..>261
                     /locus_tag="O2458_002966"
     CDS             6..>261
                     /locus_tag="O2458_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ3888745.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYSYTQSEFSGRRIEKLLHNSIRMMW"
BASE COUNT          100 a           46 c           37 g           78 t
ORIGIN      
        1 aaaaaataaa acactttgcc caacttacac taccaataga aactgctgtt agaattcctc
       61 aaaatgatat ttcgcgatat gttaatgaaa ttgttaaaaa gatagctgat agcgaattcg
      121 atgaattcag acatcatcgt ggcgcaacat cctatcatct aaaaatgatg ttaaaaatca
      181 cctcatattc atatactcaa tctgaatttt ctggccgtag aatagaaaaa ttacttcata
      241 acagtattcg aatgatgtgg t
//
LOCUS       DALAAR010000252          257 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000252 DALAAR010000000
VERSION     DALAAR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.263"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(19..>257)
                     /locus_tag="O2458_002967"
     CDS             complement(19..>257)
                     /locus_tag="O2458_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888746.1"
                     /translation="GGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDLIYIFCT
                     LLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          110 a           47 c           20 g           80 t
ORIGIN      
        1 ttacaagcgc attttcgttc agtcaattac tgccaatata acttcgtaga tcatagaaga
       61 ttgatttatt tcccagccta ttctttttat aatatttagc aaaggtaata atgatattaa
      121 taatgtacaa aaaatataaa tcaaatcgac atccttataa aacatcagaa ccactaaaaa
      181 caaaaaagca caaaataaaa ttaaatttaa aataaacgac cacttttcaa aaaaatctct
      241 tttcatattt ccacccc
//
LOCUS       DALAAR010000253          239 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000253 DALAAR010000000
VERSION     DALAAR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.265"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..>239
                     /locus_tag="O2458_002968"
     CDS             <1..>239
                     /locus_tag="O2458_002968"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCZ3888747.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGAT"
BASE COUNT           82 a           30 c           67 g           60 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgac
//
LOCUS       DALAAR010000254          226 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000254 DALAAR010000000
VERSION     DALAAR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.denovo.266"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
BASE COUNT           90 a           32 c           23 g           81 t
ORIGIN      
        1 aaaaataatg tgtgtacaaa gtcattaatt tagcaaatat ttttatttag tagttaataa
       61 ccatcgattt gaaatttata tataattatt agctaaataa tatcctgcat ctttctcata
      121 caatttacta taaaattaag catatccgat atcagcgtta ataagatcgt tgatactaga
      181 cagttaattt catagaacga aatcaaataa cacactactt tctgca
//
LOCUS       DALAAR010000255        28310 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.034, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000255 DALAAR010000000
VERSION     DALAAR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.034"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(261..1259)
                     /gene="sirC"
                     /locus_tag="O2458_002969"
     CDS             complement(261..1259)
                     /gene="sirC"
                     /locus_tag="O2458_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCZ3888748.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1256..2251)
                     /gene="sirB"
                     /locus_tag="O2458_002970"
     CDS             complement(1256..2251)
                     /gene="sirB"
                     /locus_tag="O2458_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCZ3888749.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2267..3259)
                     /gene="sirA"
                     /locus_tag="O2458_002971"
     CDS             complement(2267..3259)
                     /gene="sirA"
                     /locus_tag="O2458_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCZ3888750.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3490..4470
                     /gene="sbnA"
                     /locus_tag="O2458_002972"
     CDS             3490..4470
                     /gene="sbnA"
                     /locus_tag="O2458_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCZ3888751.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4467..5477
                     /gene="sbnB"
                     /locus_tag="O2458_002973"
     CDS             4467..5477
                     /gene="sbnB"
                     /locus_tag="O2458_002973"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCZ3888752.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5498..7252
                     /gene="sbnC"
                     /locus_tag="O2458_002974"
     CDS             5498..7252
                     /gene="sbnC"
                     /locus_tag="O2458_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCZ3888753.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7245..8501
                     /gene="sbnD"
                     /locus_tag="O2458_002975"
     CDS             7245..8501
                     /gene="sbnD"
                     /locus_tag="O2458_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCZ3888754.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8491..10227
                     /gene="sbnE"
                     /locus_tag="O2458_002976"
     CDS             8491..10227
                     /gene="sbnE"
                     /locus_tag="O2458_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCZ3888755.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10136..11986
                     /gene="sbnF"
                     /locus_tag="O2458_002977"
     CDS             10136..11986
                     /gene="sbnF"
                     /locus_tag="O2458_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCZ3888756.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11961..12737
                     /gene="sbnG"
                     /locus_tag="O2458_002978"
     CDS             11961..12737
                     /gene="sbnG"
                     /locus_tag="O2458_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCZ3888757.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12737..13939
                     /gene="sbnH"
                     /locus_tag="O2458_002979"
     CDS             12737..13939
                     /gene="sbnH"
                     /locus_tag="O2458_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCZ3888758.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13943..14707
                     /gene="sbnI"
                     /locus_tag="O2458_002980"
     CDS             13943..14707
                     /gene="sbnI"
                     /locus_tag="O2458_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCZ3888759.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14903..15310
                     /locus_tag="O2458_002981"
     CDS             14903..15310
                     /locus_tag="O2458_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888760.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15360..15986
                     /locus_tag="O2458_002982"
     CDS             15360..15986
                     /locus_tag="O2458_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ3888761.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16198..16974
                     /locus_tag="O2458_002983"
     CDS             16198..16974
                     /locus_tag="O2458_002983"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCZ3888762.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17118..17240
                     /locus_tag="O2458_002984"
     CDS             17118..17240
                     /locus_tag="O2458_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888763.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17310..18290
                     /locus_tag="O2458_002985"
     CDS             17310..18290
                     /locus_tag="O2458_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ3888764.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18253..18945
                     /locus_tag="O2458_002986"
     CDS             18253..18945
                     /locus_tag="O2458_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCZ3888765.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19155..20321
                     /locus_tag="O2458_002987"
     CDS             19155..20321
                     /locus_tag="O2458_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCZ3888766.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20302..21540
                     /locus_tag="O2458_002988"
     CDS             20302..21540
                     /locus_tag="O2458_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ3888767.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21530..22960
                     /locus_tag="O2458_002989"
     CDS             21530..22960
                     /locus_tag="O2458_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCZ3888768.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            23228..23827
                     /locus_tag="O2458_002990"
     CDS             23228..23827
                     /locus_tag="O2458_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ3888769.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            24195..24920
                     /gene="sasD"
                     /locus_tag="O2458_002991"
     CDS             24195..24920
                     /gene="sasD"
                     /locus_tag="O2458_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCZ3888770.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(25112..25867)
                     /locus_tag="O2458_002992"
     CDS             complement(25112..25867)
                     /locus_tag="O2458_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ3888771.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            26103..26810
                     /gene="deoD"
                     /locus_tag="O2458_002993"
     CDS             26103..26810
                     /gene="deoD"
                     /locus_tag="O2458_002993"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ3888772.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            26817..28169
                     /gene="tet(38)"
                     /locus_tag="O2458_002994"
     CDS             26817..28169
                     /gene="tet(38)"
                     /locus_tag="O2458_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ3888773.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         9508 a         4363 c         5383 g         9056 t
ORIGIN      
        1 tgtttttgaa taaatgacat tatcaaacac tatatttaaa taagaaatag ttatcgatat
       61 ttgaaaaatc gaagaatatt gattgatatt atagaatatg agtcggtgtt atttgtatat
      121 aaagtattaa tatgtaatta agaaagtcga cgattgttaa caataacaac aactatatgt
      181 ataaaaaaac ctcgcaacgg ttagttaact gaatcattta gctaacttcg ttgtgaggtc
      241 attttgtttt aataatatcg ttataacttt ttcacggtta ataataagta tatgaagaat
      301 ggggcaccaa aagcagcaat aaatacacct gctggcactt ctttaggcaa gaataaggta
      361 cgcccaatta agtctgcaat aacaattgat atggcaccaa tcattgctga cattagtaac
      421 tttttagcat aacttccgcg aacgattgtt ttcgcgatat gtggtgcgat taaaccgaca
      481 aacccaatgt tacctactaa actgattgcc atagatacga gtatagtaga agtgattaat
      541 tggattagtt tcatacgttg tacatgtaag cctaagccaa tcgctacagg gtcatcaagt
      601 atagatattt tcatttttgg tataacaaga aataacaacg gcacaacagc taaaataacc
      661 atacccaaaa tgattgtatc tttaaacgta gcaccgtaaa gacttccgac tagccatgta
      721 taagctttgg cagcagataa ttgcttcgtt gtaatgagta atccttggac aagcgcaata
      781 aacaacgttt gcatcgaaat accgatgatt atgagtgttg tcgggcgtat ttgtcctttc
      841 gtttgaaaca ctaatagtat catcattgca actgcgccac ctaatactgc aaatagtgga
      901 agtaaatgta ttgttaaatg gctgaaaaat gcaataaaga caacagcact taagctagca
      961 ccacctgtga taccgataat atcaggtgag gcaattggat tttttaatac attttgcaac
     1021 attaaaccac tcattcctag tgcggcacct gctaaaatcg caagtgtaat gcgaggtaag
     1081 cgtaatactt ctaaagtgaa ttgatccata ctgtcatttg gatttataaa gtacatcagt
     1141 acgcgttgta atggtataaa gcttgaacca atcatcatac ttaccactga aacgatggct
     1201 aaaaagatta acgcgaagat gagatggtaa ttgtcttttt tattaatctt ttcggtcata
     1261 agcgttgacg tcctttcttc ataatataga ttaagacaat agcgccaatg acagcggtaa
     1321 cgacaccgat aggcaactct agtggcttaa ttattatacg agcaacaatg tctgaaatga
     1381 tcattaggat tgctccagct aatgcagtaa aaggaattaa atacttatag tttggtggta
     1441 ataatcgttt gctaatattc ggtacgataa gacccacaaa gacgattgat ccagctacgg
     1501 ctaccgaaat accggctaac atactgatga gcataataat catccatttg attaatttta
     1561 tgttttgacc gaggccggtt gcaatgtcgt cacttgtcat caagatgttg atgtgtgcag
     1621 ccatgctaaa tgcaattaaa ataagtatca atacaagcgg aataatccat gggatatccc
     1681 aaatattacg taatgaaacg gagccactta accaaaataa taggccttgt aagtctgttt
     1741 cgttcataat aagtatgcct tgagtaaagg ctgtaaatag catcgcaatc gcagcacctg
     1801 ccaaaatgac acggtgaggt gagaatagtg tttgtctaaa catacctagt gcaacaacta
     1861 atacagtaac aacaatagcc cccaaaaatg caataactac aatcatttta aaagattgaa
     1921 tttggataaa tgtaatacta aaaatgacaa aaaatactgc gcctgcattg acaccgaaaa
     1981 gccctggtga ggctattggg tttcgtgtaa gtgcttgcat caacaaacct gagacagcaa
     2041 gggcagcacc agtcaataac gcaatgattg ttctcgacgc ccgtgcacca gtgacaacat
     2101 catgtaaatc gttttcacta tcaaagttga ataacgcctg tatcaccgta cctggtgaca
     2161 caagcgtatt tccaatcatt aaacttaaga tagctactat tgcaagacat aaaccagcaa
     2221 taacgatttg gtattttggt ttaagtagca tcgtaaaact ccttaattat tttgattgtt
     2281 tttcaatatt taacttttca tataaatcgt caataagttt taatgaagat ttatatccgc
     2341 cagctaagtt ccaagtgatt tcatctaaat catcagatac ttggttgttt ttaactgcgt
     2401 ctaaattttt ccactcttta cttgaagtcc attcgctttc agtcttttta actaatgcag
     2461 catctttcgc atttggatct gattttacta caaaaatatg atcagcgttc attaatggaa
     2521 tgctttcttt agatgtaagt tggataatat ctttaccatt atcaacttgt ttttgtaagt
     2581 ctttattacg tttgaatcct aaatcattta agatttcacc agcatatcca ccagcataaa
     2641 ttcttgtatg atcagcacgg aagttaacaa ctgaagcttt caatggccat gcatctttat
     2701 actttgcttt tgcatctttt tggaatgcag ctactttatc atcgtacttt ttaagtaaat
     2761 cttcagcttc tttttctttc cctaaagctt tccccattaa cttagttgta tctttgaatt
     2821 tgaaaactgt atcagtagaa actgttggtg cgattttaga taattgatcg taaacttttt
     2881 catttctaac ttttgacgcg acaattaagt ccggttttaa tttagagatt tcctctaagt
     2941 taggtgcagg ttcttgacct acaatcttag tatcttttaa atcatttttt atgtattcga
     3001 atttcggttt ttgtgtccat gattctacag cacctacagg tttaacacct aaagatacag
     3061 cgacgtcagt ggcaccttga tatagcgtaa caacacgctt tggtttccct ttaatttcag
     3121 ttgtacccat tgcatgttta attgaagttg tttccttatc tttgttatca gatgattgtt
     3181 tatttgaatt cccactacat cctgctaaaa caagtaggaa agcaagcgta acaacaagca
     3241 ttttaattac tttattcatt gactaattag cctccttcgt gatgtatgac aatgagaatc
     3301 attatcacga tttagtatga attaaatttt ttcctaagtc aataaaatat ttatgattta
     3361 catgcaactt ataattattt gacatataaa tgcataaaaa atataatcct aattacttga
     3421 tagtgagaat cattatcaat taggtaacac acaatattat agaattttaa atttgaggag
     3481 gaagcgcttt tgattgaaaa aagtcaagca tgtcacgatt cattgttaga ttctgtaggg
     3541 caaacaccta tggttcaact tcatcaacta tttccgaaac atgaagtgtt tgcaaagtta
     3601 gagtatatga atcctggagg cagcatgaaa gatcgacctg ccaagtacat cattgaacat
     3661 ggtattaaac atggtttaat cactgagaat acacatttaa ttgaaagtac ttctggtaat
     3721 ttaggcattg cgttggcaat gatagctaaa atcaagggat taaaactcac gtgtgttgtt
     3781 gatcctaaaa tatcaccaac aaatttgaaa attattaaaa gttatggtgc caatgtagaa
     3841 atggttgaag aacctgatgc acatgggggt tatttaatga ctcgtattgc aaaggtgcaa
     3901 gaactgttag ccactattga cgatgcatat tggattaatc aatatgcgaa tgagttaaat
     3961 tggcaatccc attatcatgg tgcaggcaca gagattgttg aaacaattaa gcaacctata
     4021 gattattttg tcgcgccagt cagcacgaca ggtagcatta tgggtatgag tagaaaaata
     4081 aaagaagtgc atccaaacgc acaaattgtt gctgttgatg cgaaagggtc agtcattttt
     4141 ggtgacaaac ctattaatag agaattacct ggtatcggtg ctagtcgtgt acccgaaata
     4201 ttgaatagat cagaaattaa tcaagtgatc catgtagatg attatcaatc tgctttgggc
     4261 tgtcgaaaac tgattgatta tgaaggcata tttgccggag gttcaacagg ttcgattatt
     4321 gcagcgattg agcagttgat aacgtcaatt gaagaaggtg caacaattgt cacgatttta
     4381 ccagatcgag gcgatcgtta cttagattta gtttattcag atacatggtt agaaaaaatg
     4441 aaatcaagac aaggagttaa atcagaatga atagagagat gttgtattta aatagatcag
     4501 atattgaaca agcgggaggt aatcattcac aagtttatgt ggacgcatta acagaagcat
     4561 taacagccca tgcgcacaat gattttgtac aaccgcttaa gccgtattta agacaggatc
     4621 ctgaaaatgg acacatcgca gatcgaatta ttgcaatgcc aagtcatatc ggtggtgaac
     4681 acgcaatttc aggtattaag tggataggta gtaagcacga caatccatcg aaacgtaata
     4741 tggagcgtgc aagtggcgtc attattttga atgatccaga aacgaattat ccaattgcag
     4801 ttatggaagc aagtttaatt agtagtatgc gtactgcagc agtttcagtg attgcagcaa
     4861 agcatttggc taaaaaagga tttaaagact taacaatcat tggatgcggg ctaatcggag
     4921 acaagcaatt acaaagtatg ttagagcaat tcgatcatat tgaacgcgtg tttgtttacg
     4981 atcaattctc tgaagcatgt gcacgctttg ttgatagatg gcaacaacag cgtccggaaa
     5041 ttaattttat tgcgacagaa aatgctaaag aagcagtatc aaatggtgaa gtagtcatta
     5101 catgtaccgt aacggatcaa ccatacattg aatatgattg gttacaaaag ggtgcattta
     5161 ttagcaacat ttctatcatg gatgtgcata aagaagtctt tattaaagct gacaaagtcg
     5221 tagtagatga ctggtcacaa tgtaatcgag aaaagaaaac tattaaccaa ttggtgttag
     5281 aaggtaaatt cagcaaagaa gctcttcatg ctgaactagg acaacttgtg acaggtgaca
     5341 taccaggacg tgaagacgat gatgagatca tattacttaa tccgatgggt atggctatcg
     5401 aagatatttc aagtgcttat tttatttatc aacaggcaca acaacaaaat attgggacaa
     5461 cattgaacct atattaagaa tgcgaggtgt ctgaacattg cagaatcata cagcagtcaa
     5521 tacagcacaa gcgataatat taagagattt agttgatgca ttattatttg aagatatagc
     5581 cggaattgta tcgaatagtg agattactaa agaaaacgga caaacgcttt tgatatacga
     5641 acgtgaaaca caacaaataa agatacctgt ttattttagt gctttaaata tgtttcgtta
     5701 cgaaagttca caaccaatta cgatagaggg aagggtgtct aagcaacctt taacggcagc
     5761 tgaattttgg caaacaattg ctaatatgaa ttgtgatcta agtcatgaat gggaagtggc
     5821 tcgcgttgaa gaaggactga ctactgctgc cacacagctt gctaaacaat tatcagaatt
     5881 agatttagcg tcacatcctt ttgtgatgtc agagcagttt gcaagtttaa aagatcgtcc
     5941 atttcatcca ttagctaaag aaaaaagagg attaagagaa gcggattatc aagtgtatca
     6001 agctgaatta aatcaatcat ttcctttaat ggttgcagca gttaaaaaga cacatatgat
     6061 tcatggcgat actgcaaata tcgatgaatt agaaaatttg acagtaccta taaaagaaca
     6121 agcgacagac atgttaaatg atcaagggtt atcaatagat gactatgtac tatttccggt
     6181 acatccttgg caatatcagc atattctgcc gaacgtcttt gcgaaagaga ttagtgaaaa
     6241 gttggttgta ctattaccgt taaaatttgg agattatctg tcgtcttcaa gtatgcgttc
     6301 attaattgat attggcgcac cgtataacca tgtcaaagta ccatttgcaa tgcagtcatt
     6361 aggcgcatta aggctaacgc ctacgcgtta catgaaaaac ggagaacaag cagaacaatt
     6421 attacgtcag cttatagaaa aagatgaagc actagctaag tatgtcatgg tttgtgatga
     6481 aacagcttgg tggtcatata tgggtcaaga taatgatatt ttcaaagatc aattaggtca
     6541 tctaactgtt cagctaagaa agtatcccga agtgctagcc aaaaatgata cgcaacagct
     6601 agtgtcaatg gcagcactcg cggcaaatga tcgcacttta tatcaaatga tttgtggaaa
     6661 agataatatt tctaaaaatg atgtcatgac gttatttgaa gatatcgcgc aagtcttttt
     6721 aaaggtaaca ctatcattta tgcaatacgg cgcattacca gagttgcatg gtcaaaatat
     6781 attgttgtca tttgaagatg gacgtgtaca aaaatgcgtg ttacgtgatc atgatactgt
     6841 cagaatttat aaaccatggc taacagcaca tcagctttca ttgccgaagt atgtcgtcag
     6901 agaagataca cctaatacgc taattaatga ggatttggaa acattctttg cttattttca
     6961 aacattagct gtatcggtaa atctatatgc cattattgat gcaattcaag atttatttgg
     7021 tgtaagtgag catgaactta tgtcgttgtt aaaacaaatt ttaaaaaatg aagtggcaac
     7081 tatttcctgg gttacaactg atcagctagc tgtcagacac attttatttg ataaacagac
     7141 gtggccattc aaacaaattt tattaccatt gctatatcaa cgtgatagtg gtggaggtag
     7201 tatgccttca ggtttaacta ccgtaccaaa tccaatggtg acatatgatt aatcagtcta
     7261 tatggcgcag taactttcgc attttatggc tcagtcagtt tatagcgatt gctggactga
     7321 cagtacttgt gccattattg ccaatttata tggcatcact acaaaatcta tcagtcgtag
     7381 aaatacagtt gtggagtggt atagcgattg ctgctccagc tgtaacgacg atgatagctt
     7441 cgccgatatg ggggaagcta ggtgataaga tcagccgaaa atggatggtg ttaagagcgt
     7501 tacttggttt ggcggtatgc ttatttttaa tggcattgtg tacgacacca ttacagtttg
     7561 tacttgtgag gttattgcag ggactatttg gtggtgttgt tgatgcatca agtgcgtttg
     7621 cgagtgcaga ggcgccagct gaagatcgtg gaaaggtatt aggaagactg caaagttcag
     7681 tcagcgcagg gtctcttgtg gggccattaa ttggcggtgt tacagcttcg atattaggtt
     7741 ttagtgcgtt actgatgagt attgccgtta ttacttttat tgtctgtatt ttcggtgcat
     7801 taaaattgat tgaaacgaca catatgccaa aatcacaaac accaaatatt aataaaggta
     7861 ttcgccgttc atttcaatgt ctattatgca cacaacaaac atgtcgattt attatcgttg
     7921 gcgttttagc aaactttgct atgtatggca tgctaactgc attatcacca cttgcttcat
     7981 cagtgaatca tacagcgata gatgaccgta gtgtgattgg atttttacag tccgcatttt
     8041 ggacggcttc gatattaagc gcgcctttat ggggacgctt taatgataaa tcatatgtta
     8101 aatcagtata tatatttgcc acgattgcat gtggttgtag tgcgatactg caaggtttag
     8161 cgacgaatat agagttttta atggctgcaa gaatacttca aggattaaca tatagtgcat
     8221 tgattcaaag tgtcatgttt gttgtcgtga atgcgtgtca tcaacaactt aaaggcacat
     8281 ttgttggaac gacgaacagt atgttagttg ttggtcaaat tattggcagt cttagtggcg
     8341 ctgccattac aagttatact acaccagcta ctacgtttat cgttatgggc gtagtatttg
     8401 cagtaagtag tttattttta atttgttcaa ccatcactaa tcaaatcaac gatcacacat
     8461 taatgaaatt atgggagttg aaacaaaaaa gtgcaaaata aagaattaat acaacatgca
     8521 gcgtatgcgg ctatcgaacg cattttaaat gaatatttta gagaagaaaa tttatatcaa
     8581 gtaccacctc aaaatcatca atggtctata caattatcag agctcgaaac tttaacgggt
     8641 gaatttcgct attggtctgc gatggggcat catatgtatc atccagaggt atggcttatc
     8701 gatggaaaaa gtaaaaaaat aacaacttat aaagaagcaa ttgcgcgtat tttgcaacat
     8761 atggctcaaa gtgcagataa tcaaacggca gtgcaacaac atatggcgca aattatgtct
     8821 gacatcgata atagcattca tcgcacggca cgttatttgc aaagtaacac aatagactac
     8881 gtagaggatc gttatatcgt ttcagaacaa tctttatact taggtcatcc atttcatccg
     8941 actcctaaga gtgcaagtgg gttttcagaa gcagatttag agaaatatgc acccgaatgt
     9001 catacatcat tccaattgca ttatttagct gtgcatcaag atgttctgct cacgcgctat
     9061 gtagaaggta aagaagatca ggttgagaaa gtgttgtatc aattagcaga catagatata
     9121 tcagagatac ccaaagattt tattttatta ccaacacatc cttatcaaat caatgtgttg
     9181 cgacagcatc cacagtatat gcaatatagt gaacaaggtt taataaaaga ccttggcgtt
     9241 tccggtgatt cagtgtaccc gacgtcttcg gttagaactg tattttcaaa agcattaaac
     9301 atttatttaa aattaccgat acacgttaaa atcactaatt ttatacgtac gaatgacctt
     9361 gaacagattg aacggacaat tgatgccgcg caagttatcg catcagtcaa agatgaggtt
     9421 gaaacacccc attttaaatt gatgtttgaa gaaggatatc gtgcattgtt accgaatcca
     9481 ttagggcaaa cagttgaacc tgaaatggat ttattaacaa atagtgccat gattgttcgt
     9541 gaagggatac cgaattacca tgctgataaa gatattcatg tattggcgtc attatttgaa
     9601 acgatgcctg attcaccgat gtctaagtta tcacaagtga ttgagcaaag tggtttagca
     9661 ccagaagcat ggcttgaatg ttatttgaat cgtacattat tgccgatatt aaagctgttt
     9721 agtaacacag gcattagtct agaagcacat gtacaaaata cattaattga attaaaagat
     9781 ggcatacccg acgtatgctt tgtcagagat cttgaaggca tttgtctatc tagaacgatt
     9841 gctactgaaa aacagcttgt gccaaatgtt gtggcagcat caagccctgt tgtatatgca
     9901 catgatgaag catggcatcg tcttaaatat tacgttgtag taaatcactt aggacattta
     9961 gtatcaacta ttggtaaagc gactagaaat gaagttgtgt tatggcaact tgtagcgcat
    10021 cgtcttatga cttggaaaaa agaatacgcg aataacgcag tatttgttga ctgtgtagaa
    10081 gatttatatc aaacgccgac cattgcggct aaagcgaatt tgatgagtaa attgaatgat
    10141 tgtggtgcaa accctattta tacacatata ccaaatccaa tttgtcataa caaggaggta
    10201 tcgtattgtg aatcaaacaa ttcttaatcg tgtaaagact agagtgatgc accaactggt
    10261 atcatcactt atttatgaga atattgttgt gtataaagcg tcatatcaag acggtgtcgg
    10321 tcattttaca atagaaggac atgattcaga gtatcgtttt actgctgaaa agacacatag
    10381 ctttgatcgt atacgtatca catcaccaat tgagcgtgtc gtaggagatg aggcagatac
    10441 aacaacagac tatacacaat tattgagaga ggttgtattt acatttccta aaaatgatga
    10501 aaagctagaa caatttattg tcgagttatt acagacagaa ttaaaagata cgcaaagtat
    10561 gcagtatcga gaatcaaacc caccagcaac acctgagaca tttaacgact atgaatttta
    10621 tgcgatggaa gggcatcagt atcatccaag ttacaaatca cgtttaggat ttacgttgag
    10681 tgataatttg aaatttggtc ctgattttgt accaaacgtt aaactgcagt ggttagctat
    10741 cgacaaagat aaagtagaaa cgacggtatc aagaaatgtt gtagttaacg aaatgttacg
    10801 tcaacaagtt ggcgataaga cttatgaaca ttttgtacag cagattgaag catctggcaa
    10861 acatgtaaat gatgttgaga tgatacctgt acacccatgg cagtttgaac atgtcatcca
    10921 agttgatttg gctgaagaaa ggcttaatgg cacagtacta tggttagggg aaagtgatga
    10981 gctatatcac cctcaacaat cgattcgtac gatgtcgcca atagacacga caaaatatta
    11041 tttaaaggta ccaataagta taacgaacac ttcaacgaaa cgagtgttgg cgcctcatac
    11101 aattgaaaat gcagcgcaaa ttacggattg gttaaagcag atacagcaac aagatatgta
    11161 tttaaaagat gaattaaaga cagtttttct aggggaagtc ttaggacagt cttatttaaa
    11221 tacacaactt tcgccttata aacaaactca agtttatggt gcgttaggtg ttatatggcg
    11281 tgaaaatata tatcatatgt taatcgatga agaggatgcg ataccattta atgcacttta
    11341 tgcaagtgat aaggatggtg taccattcat tgaaaattgg attaaacaat atggttctga
    11401 agcttggaca aagcaatttt tagctgtagc gattcgtcca atgattcata tgctttatta
    11461 tcacggtatt gcctttgaat cgcatgcaca aaatatgatg ctcattcatg aaaatggttg
    11521 gcctacacgt attgccttaa aagatttcca tgatggtgtt cgttttaagc gtgagcattt
    11581 aagtgaagct gcttcacacc tgacattaaa gccaatgcca gaagcacata aaaaagtgaa
    11641 tagtaattca tttattgaaa cagatgacga acgtttagta cgcgactttt tacatgatgc
    11701 atttttcttt attaatatcg ccgaaatcat cttatttatt gaaaagcaat atggtatcga
    11761 tgaggagctg caatggcaat gggttaaagg catcatcgag gcgtatcaag aagcatttcc
    11821 agagttgaat aactatcaac atttcgattt gtttgaacct acgattcaag ttgaaaagtt
    11881 aacgacacgt cgattattaa gtgactccga gttaagaatt catcatgtta caaatccatt
    11941 aggtgtagga ggtatcaatg atgcaacaac tatctctgaa acatagatta aacaatggtg
    12001 attcagttta tggcattttt aattctatac cggacccatt gatgatcgag gttatcgcag
    12061 caagcgggta tgactttgtt gtgattgata cagaacacgt ggcgattaat gatgagacac
    12121 tagcgcattt aattcgtgca gctgaagcag cgcatattat accaattgta cgtgtcactg
    12181 cagtgataga tagagatatc attaaagtgt tggatatggg tgcgagaggt attattgtgc
    12241 cacacgttaa agatcgtgag acagttgagc atattgtgaa attaagtcgt tattacccgc
    12301 aaggattaag aagtttgaat ggtggtcgca tggcaagatt tggacgtaca ccattacttg
    12361 atgcaatgga gatggctaat gagcatatta tggtgattgc catgatagaa gatgttgaag
    12421 gggttatggc cattgacgat atagcacaag tcgaaggttt agacatgata gtcgaaggtg
    12481 ccgcagattt atcgcagtca cttggcatac catggcaaac gcgtgatgat caagtaacat
    12541 cacatgttca acatattttt gaagttgtga atgcacatgg taaacatttt tgtgcattac
    12601 cacgtgaaga tgaagatatt gcaaaatggc aggcacaagg tgtacaaaca tttattttag
    12661 gtgatgatcg cggaaaaata tatcgccatt taagtgcatc tctagcgacg tctaaacaga
    12721 aaggggatga aggctaatgc gtatagttca acctgttatt gaacaattaa aagcacaatc
    12781 tcatccagtt tgtcattata tctatgattt agtcggactg gaacatcatt tgcaacatat
    12841 tacatcgtca ttgccgagta attgtcaaat gtactatgca atgaaagcaa atagtgaacg
    12901 aaaaatccta gatacaatta gtcagtatgt tgaaggattc gaagttgcat ctcaaggtga
    12961 aatagcaaaa ggtcttgctt ttaaaccagc aaatcatatt atttttggtg gccctggtaa
    13021 gacagacgag gaactaagat atgcagtaag tgaaggtgtt cagcgtattc atgttgaaag
    13081 tatgcatgaa ttacaacggc taaatgccat cttagaagat gaagataaga cacaacacat
    13141 tttattgcgt gttaatttag caggaccatt tcccaatgca acgttgcata tggcaggacg
    13201 cccaacacaa tttggtattt ctgaagacga agttgatgat gtcattgaag ctgcgctcgc
    13261 aatgccaaag attcatctag atggatttca ttttcattct atttctaaca atttagactc
    13321 gaatttacat gtcgatgtag tgaaacttta ttttaaaaaa gcaaaggcat ggtctgaaaa
    13381 acatcgattt ccactcaaac atatcaatct tggtggtggc ataggcgtta actatgcaga
    13441 tttaactaac caatttgaat gggataattt tgtagaacgt tttaaaacac ttatcgttga
    13501 gcaagaaatg gaagatgtga cattgaactt tgaatgtggg cgctttattg tggcacatat
    13561 tggttactat gtgacagaag tgctagacat taagaaagta catggtgctt ggtatgccat
    13621 attaagagga ggtacgcaac aatttagact gccggtatct tggcaacata accatccttt
    13681 tgacatttat cgctataagg acaatccata ttcatttgaa aaagtttcaa tttcgagaca
    13741 ggacacaacg ttagtcggtc aattatgtac accgaaagat gtctttgcta gagaagtaca
    13801 gatagacgca atcagtacag gcgacgttat tgttttcaaa tatgcaggtg catacggatg
    13861 gtctatttca catcacgatt tcttaagcca tccacatcct gaatttattt atttaacgca
    13921 aacaaaggag gatgaataac tattgaatca tattcatgaa catttaaaat tggtaccagt
    13981 agataagatt gatcttcacg aaacattcga acctttaaga ttggaaaaaa cgaaaagtag
    14041 tattgaagca gatgatttta tacgtcatcc tattttagtg acagcgatgc aacatggtag
    14101 atatatggtt atagatggtg tgcatcggta tacaagtttg aaagcgttag gatgtaagaa
    14161 agttccagtg caagaaatcc atgaaacaca atattcaatt agtacatggc aacataaagt
    14221 tccatttggt gtgtggtggg aaacgttaca acaagaacat cgcttgccat ggactactga
    14281 gacaagacaa gaagcgccat ttattacaat gtgtcatggt gatacagaac aatatttgta
    14341 tacaaaagat ttaggcgaag cacattttca agtatgggaa aaggttgtcg caagttatag
    14401 tggttgttgt tctgtagaga gaattgcaca aggtacatat ccttgtcttt ctcaacaaga
    14461 tgtactcatg aagtatcagc cattgagtta taaggaaatt gaagcggttg ttcataaagg
    14521 ggaaactgtg ccagcaggtg tgacacgctt taatatttca ggacgatgtc ttaatcttca
    14581 agtaccactg gcattactta aacaagatga tgatgttgaa caactgcgca attggaagca
    14641 gtttttagca gataagtttg ccaatatgag atgctatact gaaaaagtat acttggtgga
    14701 gcaatagttt tactgtgatg ttgagggaaa tatgatgatt tagcgtattg atagcgaaaa
    14761 tataataaaa caatatagtg tggagaactt ttgatatttt ataaatattg aagttctcca
    14821 tttttgtatt ttgcatataa aaattaaata aaataaggta tattaaggta aagtataaat
    14881 tttaaataaa tggggaatga gtatgagctc aattatagga aaaatagcaa tttggatagg
    14941 catcgtagct caaatatatt ttagtgtcgt ttttgttagg atgatatcta ttaatattgc
    15001 tggaggatct gattacgaaa caattttttt attaggatta atattggctc ttttcactgt
    15061 tttaccaacc atctttactg cgatttatat ggaaagttac tctgtaatcg gaggtgcact
    15121 ttttattgtt tatgctatta ttgcactgtg tttatataat ttcctttcgt caattttatg
    15181 gctgattggt ggtattttgc tgatttggaa taaatactca aaagatgaat cgacagacga
    15241 aaatgaaaaa gttgatattg aaagtacaga gaatcaattt gaatctaaag ataaaatcac
    15301 taaagaataa agagaatatt taaggtaaag tataaatttt aaataaatgg ggaatagaca
    15361 tggaaaaaaa tgtagaaaaa tcattcataa agataggttt atattttcaa atagcttata
    15421 tagtactcat ggctataact ttatgtgggt ttgtaatttg ctatggacta attttcggcc
    15481 ttttctattt attatcaggt agcagagctg attatttaat agtaacaata gttatatcgg
    15541 caataatttc tatatttgta attatacttt caatcgtacc tgtcatcgta ttggcatctg
    15601 acttatttaa agaaaggatt tcaaaaggtg tcatattaat tgtattggct attatcgctt
    15661 tagtattatg caactttgta tctgcaatac tctggtttgt ttcagccata tctattttag
    15721 gtagaaaaaa attagtagct gcagcagata ctaccactat tcaaaaaagt aaagggaacg
    15781 caaatcaagc atcacataaa gacacgtgta aaaaggaact tgatagtcaa gacatgatgg
    15841 aacatcctga ggttaaaaat cccacgacta aaaaccttga aggatttaac gaagaaatac
    15901 ataaagatga agctacaact aaagttgtca gtgataacac ggaaccgcct attgaatcaa
    15961 aagaccatgt ctcgaaaaaa gattgatgac aaactaatcg agagacttaa aaaaataata
    16021 ttcaacataa gaacttttaa aacgacattt aaacgcattg ccaatcacta atggtagtgc
    16081 gtttaactat accttaaata tctgaatatt ttgttaaatg gagctacctt tgttgtacta
    16141 ttcaaatgaa gaggagtaaa atgtaattaa aggaaagaaa tttgaggagt gatctttatg
    16201 acaaacaaca aagtagcatt agtaactggc ggagcacaag ggattggttt taaaattgca
    16261 gaacgtttag tggaagatgg tttcaaagta gcagttgttg atttcaatga agaaggggca
    16321 aaagcagctg cacttaaatt atcaagtgat ggtacaaaag ctattgctat caaagcagat
    16381 gtatcaaacc gtgatgatgt atttaacgca gtaagacaaa ctgccgcgca atttggcgat
    16441 ttccatgtca tggttaacaa tgccggcctt ggaccaacaa caccaatcga tacaattact
    16501 gaagaacagt ttaaaacagt atatggcgtg aacgttgcag gtgtgctatg gggtattcaa
    16561 gccgcacatg aacaatttaa aaaattcaat catggcggta aaattatcaa tgcaacatct
    16621 caagcaggcg ttgagggtaa cccaggcttg tctttatatt gcagtacaaa attcgcagtg
    16681 cgaggtttaa cacaagtagc cgcacaagat ttagcgtctg aaggtattac tgtgaatgca
    16741 ttcgcacctg gtatcgttca aacaccaatg atggaaagta tcgcagtggc aacagccgaa
    16801 gaagcaggta aacctgaagc atggggttgg gaacaattta caagtcagat tgctttgggc
    16861 agagtttctc aaccagaaga tgtttcaaat gtagtgagct tcttagctgg taaagactct
    16921 gattacatta ctggacaaac aattattgta gatggtggta tgagattccg ttaataatca
    16981 tccactaatg ataaataaat ccttattgtt aagtttaatc acttagcagt aaggattttt
    17041 tagtgcactt agaagggagt gtattggtag aaaattaata agcgaagttc ttaagtgagt
    17101 tatgatgtca cagtctaatg catcagttga aagcattatt agtattaaca cacccaagat
    17161 attataaaac atcacaaaaa caccactatc taatttatct caataaaaat tcacaaagtt
    17221 atctcatttt atttttataa ataaaaaata tcgataaaaa gcttacaata ctttatgttt
    17281 ttatgatata tttttaatgt ataaatgagg tggaagattt ggaaagagtt ttgataactg
    17341 gtggggctgg ttttattggg tcgcatttag tagatgattt acaacaagat tatgatgttt
    17401 atgttctaga taactataga acaggtaaac gagaaaatat taaaagtttg gctgacgatc
    17461 atgtgtttga attagatatt cgtgaatatg atgcagttga acaaatcatg aagacatatc
    17521 aatttgatta tgttattcat ttagcagcat tagttagtgt tgctgagtcg gttgagaaac
    17581 ctatcttatc tcaagaaata aacgtcgtag caacattaag attgttagaa atcattaaaa
    17641 aatataataa tcatataaaa cgttttatct ttgcttcgtc agcagctgtt tatggtgatc
    17701 ttcctgattt gcctaaaagt gatcaatcat taatcttacc attatcacca tatgcaatag
    17761 ataaatatta cggcgaacgg acgacattaa attattgttc gttatataac ataccaacag
    17821 cggttgttaa attttttaat gtatttgggc caagacagga tcctaagtca caatattcag
    17881 gtgtgatttc aaagatgttc gattcatttg agcataacaa gccatttaca ttttttggtg
    17941 acggactgca aactagagat tttgtatatg tatatgatgt tgttcaatct gtacgcttaa
    18001 ttatggaaca caaagatgca attggacacg gttataacat tggtacaggc acttttacta
    18061 atttattaga ggtttatcgt attattggtg aattatatgg aaaatcagtc gagcatgaat
    18121 ttaaagaagc acgaaaagga gatattaagc attcttatgc agatatttct aacttaaagg
    18181 cattaggatt tgttcctaaa tatacagtag aaacaggttt aaaggattac tttaattttg
    18241 aggtagataa tattgaagaa gttacagcta aagaagtgga aatgtcgtga aaatgacatt
    18301 gaagctgtcc ataataataa gggttatgcc tatcaaagaa aattagacaa actagaagaa
    18361 gtgagaaaaa gctattaccc aattaaacgt gcgattgact taattttaag cattgtttta
    18421 ttatttttaa ctttaccgat tatggttata ttcgccattg ctatcgtcat agattcgcca
    18481 ggaaacccta tttatagtca ggttagagtt gggaagatgg gtaaattaat taaaatatac
    18541 aaattacgtt cgatgtgcaa aaacgcagag aaaaacggtg cgcaatgggc tgataaagat
    18601 gatgatcgta taacaaatgt cgggaagttt attcgtaaaa cacgcattga tgaattacca
    18661 caactaatta atgttgttaa aggggaaatg agttttattg gaccacgccc ggaacgtccg
    18721 gaatttgtag aattatttag ttcagaagtg ataggtttcg agcaaagatg tcttgttaca
    18781 ccagggttaa caggacttgc gcaaattcaa ggtggatatg acttaacacc gcaacaaaaa
    18841 ctgaaatatg acatgaaata tatacataaa ggtagtttaa tgatggaact atatatatca
    18901 attagaacat tgatggttgt tattacaggg gaaggctcaa ggtagtctta atttacttaa
    18961 taagttcaaa taaaagttat attttaaaga ttgtgaccaa ttgttacagt ataacgagga
    19021 atcccttgag acagtatcaa atggcattaa gaaatatgtg ccatcattga tttgcatggc
    19081 tataaatact attcatctga tgagatagcc atgttaagaa attgaaagta tagcattaaa
    19141 ggggtttgta acagttgaaa attatatatt gtattactaa agcagacaat ggtggtgcac
    19201 aaacacatct cattcaactc gccaaccatt tttgcgtaca caatgatgtt tatgtcattg
    19261 taggcaatca tggaccaatg attgaacaac tagatgcaag agttaatgta attattatcg
    19321 aacatttagt aggtccaatt gactttaaac aagatatttt agctgtcaaa gtgttagcac
    19381 agttattctc gaaaattaaa cctgatgtta tccatttaca ttcttccaaa gctggaacgg
    19441 tcggacgaat tgcgaagttc atttcgaaat cgaaagacac acgtatagtt tttactgcac
    19501 atggatgggc ttttacagag ggtgttaaac cagctaaaaa atttctatat ttagttatcg
    19561 aaaaattaat gtcacttatt acagatagca ttatttgtgt ttcagatttc gataaacagt
    19621 tagcgttaaa atatcgattt aatcgattga aattaaccac aatacataat ggtattgcag
    19681 atgttcccgc tgttaagcaa acgctaaaaa gccaatcaca taacaatatt ggcgaagtag
    19741 ttggaatgtt gcctaataaa caagatttac agattaatgc cccgacaaag catcaatttg
    19801 ttatgattgc aagatttgct tatccaaaat tgccacaaaa tctaatcgcg gcaatagaga
    19861 tattgaaatt acataacagt aatcatgcgc attttacatt tataggcgat ggacctacat
    19921 taaatgattg tcagcaacaa gttgtacaag ctgggttaga aaatgaagtc acatttttgg
    19981 gcaatgtcat taatgcgagt catttattat cacaatacga tacgtttatt ttaataagta
    20041 agcatgaagg tttgccaatt agcattatag aagctatggc tacaggtttg cctgttatag
    20101 ccagtcatgt tggcggtatt tcagaattag tagctgataa tggtatatgt atgatgaaca
    20161 accaacccga aactattgct aaagtcctgg aaaaatattt aatagacagt gattacatca
    20221 aaatgagtaa tcaatctaga aaacgttatt tagaatgttt tactgaggag aaaatgatta
    20281 aagaagtgga agacgtttat aatggaaaat caacacaata gtaaattact aacattgtta
    20341 cttatcggtt tagcggtttt tattcagcaa tcttcggtta ttgccggtgt gaatgtttct
    20401 atagctgact ttatcacatt actaatatta gtttatttac tgtttttcgc taaccattta
    20461 ttaaaggcaa atcatttttt acagtttttc attattttgt atacatatcg tatgattatt
    20521 acgctttgtt tgctattttt tgatgatttg atatttatta cggttaagga agttcttgca
    20581 tctacagtta aatatgcatt tgtagtcatt tatttctatt tagggatgat catctttaag
    20641 ttaggtaata gcaaaaaagt gatcgttacc tcttatatta taagcagtgt gactataggt
    20701 ctattttgta ttatagctgg tttgaacaag tcccctttac taatgaaatt gttatatttt
    20761 gatgaaatac gttcaaaagg attaatgaat gaccctaact atttcgcgat gacacagatt
    20821 attacattgg tacttgctta caagtatatt cataattaca tattcaaggt ccttgcatgt
    20881 ggtattttgc tatggtcttt aactacaacg gggtctaaga ctgcgtttat catattaatc
    20941 gtcttagcca tttatttctt tattaaaaag ttatttagta gaaatgcggt aagtgttgtg
    21001 agtatgtcag tgattatgct gatattactt tgttttacct tttataatat caactactat
    21061 ttattccaat taagcgacct tgatgcctta ccgtcattag atcgaatggc gtctattttt
    21121 gaagagggct ttgcatcatt aaatgatagt gggtctgagc gaagtgttgt atggataaat
    21181 gccatttcag taattaaata tacactaggt tttggtgtcg gattagtgga ttatgtacat
    21241 attggctcgc aaattaatgg tattttactt gttgcccata atacatattt gcagatcttt
    21301 gcggaatggg gcattttatt cggtgcatta tttatcatat ttatgcttta tttactgttt
    21361 gaattattta gatttaacat ttctgggaaa aatgtaacag caattgttgt aatgttgacg
    21421 atgctgattt actttttaac agtatcattt aataactcaa gatatgtcgc ttttatttta
    21481 ggaattatcg tctttattgt tcaatatgaa aagatggaaa gggatcgtaa tgaagagtga
    21541 ttcactaaaa gaaaatatta tttatcaagg gctataccaa ttgattagaa cgatgacacc
    21601 actgattaca atacccatta tttcacgtgc atttggtccc agtggtgtgg gtattgtttc
    21661 attttctttc aatatcgtgc aatacttttt gatgattgca agtgttggcg ttcagttata
    21721 ttttaataga gttatcgcga agtccgttaa cgacaaacgg caattgtcac agcagttttg
    21781 ggatatcttt gtcagtaaat tatttttagc gttaacagtt tttgcgatgt atatggtcgt
    21841 aattactata tttattgatg attactatct tattttccta ctacaaggaa tctatattat
    21901 aggtgcagca ctcgatattt catggtttta tgctggaact gaaaagttta aaattcctag
    21961 cctcagtaat attgttgcgt ctggtattgt attaagtgta gttgttattt ttgtcaaaga
    22021 tcaatcagat ttatcattgt atgtatttac tattgctatt gtgacggtat taaaccaatt
    22081 acctttgttt atctatttaa aacgatacat tagctttgtt tcggttaatt ggatacacgt
    22141 ctggcaattg tttcgttcgt cattagcata cttattacca aatggacagc tcaacttata
    22201 tactagtatt tcttgcgttg ttcttggttt agtaggtaca taccaacaag ttggtatctt
    22261 ttctaacgca tttaatattt taacggtcgc aatcataatg attaatacat ttgatcttgt
    22321 aatgattccg cgtattacca aaatgtctat ccagcaatca catagtttaa ctaaaacgtt
    22381 agctaataat atgaatattc aattgatatt aacaatacct atggtctttg gtttaattgc
    22441 aattatgcca tcattttatt tatggttctt tggtgaggaa ttcgcatcaa ctgtcccatt
    22501 gatgaccatt ttagcgatac ttgtattaat cattccttta aatatgttga taagcaggca
    22561 atatttatta atagtgaata aaataagatt atataatgcg tcaattacta ttggtgcagt
    22621 gataaaccta gtattatgta ttattttgat atatttttat ggaatttacg gtgctgctat
    22681 tgcgcgttta attacagagt ttttcttgct catttggcga tttattgata ttactaaaat
    22741 caatgtgaag ttgaatattg taagtacgat tcaatgtgtc attgctgctg ttatgatgtt
    22801 tattgtgctt ggtgtggtca atcattattt gccccctaca atgtacgcta cgctgctatt
    22861 aattgcgatt ggtatagtag tttatctttt attaatgatg actatgaaaa atcaatacgt
    22921 atggcaaata ttgaggcatc ttcgacataa aacaatttaa gtaccggtaa tgctatactt
    22981 tagaaaatta agattaagaa gaaaaggcaa tttcttattg aaaaatggaa gttgtctttt
    23041 ttaattctct ttaaaagcgg gaaacaaaag cagttaaatg cctttttgca ttcaatatta
    23101 aatattatat caatttcgaa tatttaaatt ttatataatt ggatataaca aataaataat
    23161 aattattgca aaacacaccc gaaattaatt attataaaag tatattcata aaaggaggaa
    23221 tatacttatg gcatttaaat taccaaattt accatatgca tatgatgcat tggaaccata
    23281 tatagatcaa agaacaatgg agtttcatca cgacaaacat cacaatacgt acgtgacgaa
    23341 attaaacgca acagttgaag gaacagagtt agagcatcaa tcactagcgg atatgattgc
    23401 taacttagac aaggtaccgg aagcgatgag gatgtcagtc cgtaataatg gcggtggtca
    23461 ttttaaccat tcattattct gggaaatact atcacctaat tctgaagaaa aaggtggcgt
    23521 aatagatgac atcaaagcgc agtggggcac tttagatgaa tttaaaaatg aatttgcaaa
    23581 taaagcaaca acattatttg gatcaggttg gacttggtta gttgttaatg atggcaaatt
    23641 agaaattgtg acaacgccaa accaagataa tccattaaca gaaggcaaaa caccaatctt
    23701 actatttgat gtttgggagc atgcctacta tctgaaatat caaaataaac gtccagacta
    23761 tatgactgca ttttggaaca ttgttaactg gaaaaaagtt gatgaattat accaagcagc
    23821 aaaataatat aacttaatat aattgaggtg gagcatctac aaggtgttcc acttttttgt
    23881 acttaatatc tttacttatt tgtttttgtg taatgggata gcacgtaaaa gtggaaaaat
    23941 aataagataa atttcaactc gaaatacctt attattgttg actataacga tgacctaaat
    24001 attatataca ttagacaaca ctattaatta atcaacatat tgacattaaa taaattgaca
    24061 aaataagtaa ttattgtgaa tctaaagtga aatttttata aaaaaatgta atgattcaaa
    24121 aattttgttg catttctttt gtaatcgtat gataatgtaa atgtaatcaa attgtaatat
    24181 aaggggacaa gacaatgaaa aaattagcaa cagtaggttc tttaattgta acaagcactt
    24241 tagtattctc aagtatgcct tttcaaaatg cgcatgccga cacaacttca atgaatgtgt
    24301 cgaataaaca aagccaaaat gtacaaaatc atcgtcctta tggcggagta gtaccacaag
    24361 gaatgacgca agcacaatat actgaattag agaaagcttt accccaatta agcgctggca
    24421 gtaatatgca agactataat atgaaattgt atgatgcgac gcaaaatatt gctgataaat
    24481 acaatgtgat aattacaact aatgtagggg tatttaaacc acatgctgtt agagatatga
    24541 atggccatgc gttaccttta acaaaagatg gcaattttta tcaaacgaat gtagatgcaa
    24601 atggtgttaa tcatggtggt agtgaaatgg tgcaaaataa aacaggtcat atgagtcaac
    24661 aaggccatat gaatcagaac acacacatga accaacagcc acacatgcaa caaggtcata
    24721 tgcaatcatc aaaccatcaa atgatgagtc caaaagcaaa tatgcattca tcaaatcatc
    24781 aaatgaacca aagtaacaaa aaagttttac cagctgctgg tgaaagtatg acatcaagta
    24841 ttcttactgc aagtattgcc gcactactat tagtatctgg gttattctta gcatttagac
    24901 gacgttcaac aaataaataa acataatacg attaataata gaaaaatcgt gtgattatct
    24961 gagcgagcct aggacataaa tcaatgtcct aggctcgcta atgttatatt ggcagtagtt
    25021 gactgaatga aattgcgctt gtaacaagct tttccatttc ttaccaactc cctaaatagt
    25081 catcaaaaaa tttcttatat tttaataatt tttaataatc cgattgtctt atacgtgtca
    25141 gtgttaattc agatatttcc tgtggaatat accacttatt aatcataatt ggataaggtg
    25201 tttgtgcgta cagtgtttca ataatcagcc aacaatgtgt atcaccatca aacacgtgac
    25261 tatgattttt gaagtggggc gctttggtaa tagacatttt taaatctgat tgatatgcat
    25321 tgctataaat cgtttgctca acgaatgtct tcatgtcgtc ttcgttttgt gtattcactt
    25381 taaatgtgtc aatgacattt aacggtataa aggtaaagca aaatgcatca gcttgcttag
    25441 aatgattgtc ctttttttga taatagcgtt ccattgcaat gacggcagaa ggatggtttg
    25501 caaacaaatg atttgtatat tcactttcta aatcaacacg ataattaatt gatgacatag
    25561 atacgcgagc tagcaatatt tgatcaagtg gatgcttaaa ttgatccata cttgaagcgt
    25621 gttgggcatt tgtttgtgga ataacaaagt gtcccttccc tcttgtactc tctacgatgc
    25681 catcttcggc taacaatttt atagcttggc gcaaagtcat acgactgaca tcaaagcgcg
    25741 cacaaagttc cttttcagta ggtaatgcat ggccactcgg atattttcct atttgaattt
    25801 ctttatataa cgtattataa atcgttaaaa attttggttg tgtttgcgtc acgtagacaa
    25861 cctccataaa gttacttaat cactctcatc atacaataat ttttactcaa attggaaaaa
    25921 ttataaaaat taaatataga taggctttga aaattagttt tatacaaggt tagtagctgt
    25981 aactgtaaaa tgttcttaat attgtcaaaa tgtaatgctt gaaagcgctt ttaaaaaata
    26041 ttattatata catggttaga caaatagaca aatcactata caaatattgg gaggaatatt
    26101 ttatgaaatc aacaccacac attaaaccaa tgaatgacgt cgaaattgca gaaacggttc
    26161 tattgccagg agatccgtta agagctaagt tcattgcaga aacttatttg gatgatgtgg
    26221 aacagttcaa tacagtgcga aacatgtttg gttttaccgg aacatataaa ggtaaaaaag
    26281 tttctgtcat gggttcaggt atgggtatgc catctattgg catttactct tatgaattaa
    26341 ttcatacatt tggttgtaaa aaattaattc gcgttggctc ttgtggcgcg atgcaagaaa
    26401 acattgattt atatgatgtg attattgcac aaggtgcctc tactgattca aattacgttc
    26461 aacaatatca attaccaggt cattttgcgc caattgcttc ttatcaatta ttagaaaaag
    26521 cagttgaaac agcacgtgac aaaggtgtac gtcatcatgt aggtaatgtg ttatcaagtg
    26581 atattttcta taacgcggat acaacagcga gtgaacgttg gatgcgtatg ggtattttag
    26641 gtgtagaaat ggaatcagct gcattataca tgaatgcaat ttacgctggt gtcgaagcat
    26701 taggtgtgtt cacagtgagc gatcatttaa ttcatgaaac gtcaacaaca cctgaggaaa
    26761 gggaacgtgc atttacagat atgattgaaa ttgcactgtc attggtgtag atgattatga
    26821 atgttgaata ttctaaaata aagaaagcag tacctatttt attattctta tttgtattca
    26881 gtttggttat agacaactca tttaaattga tttctgtagc cattgctgat gacttaaaca
    26941 tatctgtaac gacagtaagt tggcaagcga cattagccgg tttagtaatt ggtattggcg
    27001 ctgtagtata cgcttcatta tctgatgcca ttagtatacg cacactattt atttatggcg
    27061 tgatattaat cattatcgga tcaattattg gttacatttt ccaacatcaa ttcccattac
    27121 ttttagttgg acgtattatt caaactgccg gtttagctgc tgcagagaca ttatatgtga
    27181 tatatgttgc aaagtatctt tctaaagagg accagaagac ttaccttggc ttaagtacga
    27241 gcagttattc cttgtcatta gttatcggta cattatcagg tggatttatt tctacgtatt
    27301 tacactggac aaatatgttt ttaattgcat taatcgtagt atttacgttg ccattcctat
    27361 ttaaattatt accaaaagaa aataatacga ataaagctca tttagatttt gttggcttaa
    27421 ttctagtggc aactattgct acaacagtca tgctgtttat tacgaacttt aattggttat
    27481 atatgattgg tgccttaatt gcgattatcg tttttgcgct atatattaaa aatgcgcaac
    27541 gtccattagt aaataaatca tttttccaaa ataaacgtta tgcttcattt ttatttatag
    27601 tatttgtaat gtatgctatc caattgggtt atatttttac gttcccattc ataatggagc
    27661 aaatttatca tctgcaacta gacacaacat cactgttatt agtaccgggt tatatagtag
    27721 cagtcattgt tggtgcacta agtggtaaaa tcggcgaata tctgaattca aaacaagcga
    27781 ttatcacagc aattatttta atagcactga gcttgatttt acctgcattt gcagtaggta
    27841 atcacatttc aatcttcgtc atttctatga tattctttgc aggtagcttt gctttaatgt
    27901 atgcaccttt acttaacgaa gccattaaaa caatagatct taatatgaca ggtgtggcta
    27961 ttggttttta taatttaatt attaatgtgg cggtatctgt aggtattgcg attgctgcgg
    28021 ctctaatcga ttttaaagca ttaaatttcc caggcaatga tgcattaagt tcacatttcg
    28081 gtattatttt aattatttta ggtttaatga gtattgtcgg attagtttta ttcgtcagct
    28141 taaatcgttg gacacaatct gaaaaataaa tagatataaa ttcgcgagat atattcgtat
    28201 ttatagtaaa attaaataaa gagattatat aacacgagga gtagtaagta tgaaatttga
    28261 gaaatatata gatcacactt tattgaagcc tgagtcaaca cgtacgcaaa
//
LOCUS       DALAAR010000256        25687 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.038, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000256 DALAAR010000000
VERSION     DALAAR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.038"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            10..429
                     /locus_tag="O2458_002995"
     CDS             10..429
                     /locus_tag="O2458_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ3888774.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(685..1620)
                     /locus_tag="O2458_002996"
     CDS             complement(685..1620)
                     /locus_tag="O2458_002996"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCZ3888775.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            1910..3169
                     /gene="sdpC"
                     /locus_tag="O2458_002997"
     CDS             1910..3169
                     /gene="sdpC"
                     /locus_tag="O2458_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCZ3888776.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(3235..3921)
                     /locus_tag="O2458_002998"
     CDS             complement(3235..3921)
                     /locus_tag="O2458_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCZ3888777.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            4095..4748
                     /locus_tag="O2458_002999"
     CDS             4095..4748
                     /locus_tag="O2458_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCZ3888778.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            complement(4984..6003)
                     /locus_tag="O2458_003000"
     CDS             complement(4984..6003)
                     /locus_tag="O2458_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCZ3888779.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(6033..6359)
                     /locus_tag="O2458_003001"
     CDS             complement(6033..6359)
                     /locus_tag="O2458_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCZ3888780.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            complement(6408..6593)
                     /locus_tag="O2458_003002"
     CDS             complement(6408..6593)
                     /locus_tag="O2458_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888781.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            complement(6577..7809)
                     /locus_tag="O2458_003003"
     CDS             complement(6577..7809)
                     /locus_tag="O2458_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888782.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            complement(7802..8701)
                     /locus_tag="O2458_003004"
     CDS             complement(7802..8701)
                     /locus_tag="O2458_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888783.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            complement(8932..9579)
                     /locus_tag="O2458_003005"
     CDS             complement(8932..9579)
                     /locus_tag="O2458_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCZ3888784.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            9978..10586
                     /locus_tag="O2458_003006"
     CDS             9978..10586
                     /locus_tag="O2458_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCZ3888785.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            10923..12131
                     /gene="gltS"
                     /locus_tag="O2458_003007"
     CDS             10923..12131
                     /gene="gltS"
                     /locus_tag="O2458_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCZ3888786.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(12179..13228)
                     /locus_tag="O2458_003008"
     CDS             complement(12179..13228)
                     /locus_tag="O2458_003008"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCZ3888787.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(13259..14206)
                     /gene="corA"
                     /locus_tag="O2458_003009"
     CDS             complement(13259..14206)
                     /gene="corA"
                     /locus_tag="O2458_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCZ3888788.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            14303..14794
                     /locus_tag="O2458_003010"
     CDS             14303..14794
                     /locus_tag="O2458_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCZ3888789.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            14791..15210
                     /locus_tag="O2458_003011"
     CDS             14791..15210
                     /locus_tag="O2458_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888790.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(15318..16238)
                     /locus_tag="O2458_003012"
     CDS             complement(15318..16238)
                     /locus_tag="O2458_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ3888791.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            16446..17084
                     /locus_tag="O2458_003013"
     CDS             16446..17084
                     /locus_tag="O2458_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888792.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(17330..19261)
                     /locus_tag="O2458_003014"
     CDS             complement(17330..19261)
                     /locus_tag="O2458_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCZ3888793.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(19274..19921)
                     /locus_tag="O2458_003015"
     CDS             complement(19274..19921)
                     /locus_tag="O2458_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCZ3888794.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            20042..20593
                     /locus_tag="O2458_003016"
     CDS             20042..20593
                     /locus_tag="O2458_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ3888795.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(20823..22031)
                     /locus_tag="O2458_003017"
     CDS             complement(20823..22031)
                     /locus_tag="O2458_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ3888796.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(22141..22269)
                     /locus_tag="O2458_003018"
     CDS             complement(22141..22269)
                     /locus_tag="O2458_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888797.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(22300..23682)
                     /locus_tag="O2458_003019"
     CDS             complement(22300..23682)
                     /locus_tag="O2458_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888798.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(23922..24377)
                     /locus_tag="O2458_003020"
     CDS             complement(23922..24377)
                     /locus_tag="O2458_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3888799.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            24572..25540
                     /locus_tag="O2458_003021"
     CDS             24572..25540
                     /locus_tag="O2458_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCZ3888800.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT         8493 a         4572 c         3576 g         9046 t
ORIGIN      
        1 gtgaagcaga tgttaaaatc tattaatcat atatgctttt cagtcagaaa tttaaacgat
       61 tcaatacatt tttatagaga tattttactt gggaaattgc tattgactgg taaaaaaact
      121 gcttattttg agcttgcagg cctatggatt gctttaaatg aagaaaaaga tataccacgt
      181 aatgaaattc acttttcata tacacatata gctttcacta tagatgacag cgaatttaaa
      241 tattggcatc agaggttaaa agataataac gtgaatattt tagaaggaag agttagagat
      301 attagagata gacaatcaat ttactttacc gaccctgatg gtcataagct agaattacat
      361 actggcacac ttgagaacag attaaattat tataaagagg ctaaaccaca tatgacattt
      421 tacaaataag gtgtcattat aaaaaggcct cttgaactcc gttaaaattt taattaatta
      481 ttatataata agagaacttt tcaaacaata cagttgttat ttttgctatt tcaacaaaca
      541 taaataagca gtaagatgac tacaacttaa gagtcttctt actgcaatta tttttcaaat
      601 atatcaacgt taatataact tctattaaga aatactcaca ttctgccctg caatgcaaat
      661 ctcgtcacat ataaatattt ttaattattt taaaaaatga tgcactaaat tagcaacgag
      721 cttagcagtt ctattgtcag cgtcatatgt tggattcatc tcagcaatac taactgaaga
      781 caccttatca cttggaataa tacgttttgc taattcaaga acagtatgtg gatacaaacc
      841 taacactgcc ggcgcactta ccccaggcgc aaacgcacta tcaatgacat ccatacaaat
      901 cgtaaacata atgacatcat gttcatgtac aaaacgttca atcatatctt taattgttgg
      961 tgatacgtga ctcaataatt catctgcaaa gacataatca atctttttct ctttagcata
     1021 atcaaataaa ctttgcgtat taccaccttg agcaatacca agcactaaat aatctgtgtt
     1081 ttcatcttct tctaaaattt gtctaaagct cgttccagat gtagattgtt gttcagcacg
     1141 tgtatcaaaa tgcgcatcaa tatttatcac accaatagat tgtgttggat agactttacg
     1201 tgttgctaaa tattgagcat acgcaatatc atgtccacca cctaataaaa atgtttgtct
     1261 atgattagca attgacttcg ctgcaagcat agcaaattct ttttgagtat caattaattc
     1321 ctcatgatca tgataaacat ttccgtaatc gactaaagtt tcacattgat tcaaatccgg
     1381 caaacctgca aatgcttgtt taatcgcatc tggtccttct tttgcaccaa tgcgcccctt
     1441 gtttaaagca acacctttgt caacagcata gcctaatata ccgacccctg atggcatact
     1501 actcttttcc agcttagaca aatcttcaaa tgttactgtt tgaaaatgtc taaatttttt
     1561 cgggtctgtt tcactatcta accttccagt ccataaattt ggttcacctt gcttgtacac
     1621 agcatttccc cctcttattt atgtggctta ttaacaatta aagtataacg tataggaaat
     1681 tttgaattca attcatagtt aaatccgtat cttaaaaata cttatctaca ttacttttac
     1741 ccctattttc tatgtaataa cgaatactta gctgatttat gttaataaaa tacgtcaaga
     1801 ctattacatt ttcattaata ttgacataga caatttatct ctcggcttgt aatatgtata
     1861 attgttacta aaagatattt tgcttgttac ctaatggagg ttacatataa tgaagaacaa
     1921 taaaatttct ggttttcaat gggcaatgac gattttcgtc ttctttgtca ttacaatggc
     1981 gttatccatt atgctcagag atttccagtc tataattggt gtcaaacact ttatatttga
     2041 agttacagat ctagcaccat taattgctgc aatcatttgt atactcgttt tcaaatataa
     2101 aaaggtccaa cttgcaggtt taaaattctc aatcagcctg aaagtaattg aacgtctatt
     2161 gctagcttta attttacctt taattattct aattattggt atgtacagct ttaatacatt
     2221 tgcagatagc tttattttat tacaatcaac aggcttatca gtacctatta cacacattct
     2281 gattggacat attctgatgg cgttcgtagt agaattcgga ttccgttcat acttacaaaa
     2341 tattgttgaa acgaaaatga acacgttctt tgcaagtatt gttgttggtt tgatgtattc
     2401 agttttctca gcaaatacaa cttatggtac agaatttgct gcttataact tcctttatac
     2461 attctcattc tctatgattc ttggtgaatt aattagagcg actaaaggac gtacaattta
     2521 tattgcaacg acattccatg cttcaatgac attcggactt attttcttgt ttagcgaaga
     2581 aatcggcgat ctattttcaa tcaaagtcat cgccatttca acagcaatcg ttgcagtagg
     2641 atacattggt ttaagcttaa ttatccgagg tattgcatat ttaacaacaa gacgaaacct
     2701 tgaagaactt gagcctaata attatttaga ccatgtcaat gacgatgaag aaactaatca
     2761 tactgaggct gaaaaatctt cttcaaatat taaagatgct gaaaaaacag gtgtagctac
     2821 tgcatcaacg gttggtgttg ctaaaaatga tactgaaaat acagtggctg acgaaccaag
     2881 cattcatgaa ggtactgaaa aaacagaacc tcaacatcac ataggtaatc aaactgaatc
     2941 taatcatgat gaagatcatg acatcacttc ggagtcagta gaatcagcag aatcagttaa
     3001 acaagcacca caaagtgacg atttaacaaa cgattcaaat gaagatgaaa tagagcaatc
     3061 attaaaagaa cctgcgactt ataaagaaga cagacgttca tcagttgtaa ttgatgcaga
     3121 aaaacatatc gaaaaaactg aagaacaatc ttcagataaa aacaaataat aacaaagcgc
     3181 ttgctagtac ctcttaaaaa gatgatgcta gcaagcgctt ttctatacta tatattattt
     3241 ttctaaaatt ttaacaccct cttgagtgcc tacaataact tgatctgcca tatctaagaa
     3301 gtatcctgtc tcaaacacac ctgtcagatg aattaaatac tcatggaact tatatgggtc
     3361 aattcctttt ggtaacttac aatctaaaat ataattgcca ttatcagtta taaatgcgac
     3421 atcttcgttt acacgacgtt ctacttttat atcagcgtat gattcaattt tacgtaatat
     3481 gtgataccag ttaaatttat ccacctctac tggtaactta aacgtctcac ctaagtattg
     3541 aactattttc gtttcatcga caaccacaac aaaacgcgat gccatttcat ctataacttt
     3601 ctctctgaac agcgcaccac cgccaccttt aattatattt aaagatggat ctacttcatc
     3661 agcaccatca attgctaagt cgatatgatc aacatcattg atttcacata ttttaatacc
     3721 taattctttt gctaaaaatg caattttatt agaagtgcat acacctgtaa tattgtaacc
     3781 acgttcttta attagttgcg ccatttgagg taagagtaat tccattgtac ttcctgtacc
     3841 aattcccagc gtcatgtcac cattgatttg acttaaaaca tcatttaatg tcattaactt
     3901 gagtgctttg acatctttca tgaaggtagc ctcccatatt tatgtaatct attcaattca
     3961 tattttacat gactcgtata aattaacata cccttatttc taacattttt ttaaacatat
     4021 caatcagaat ttacaattct aaaaatgttt tatagtacaa tatattttaa ataatactcg
     4081 ttaaggagaa tgatatgata tcaattcacg caatttcgac aggaaaaatc caagatttgc
     4141 cttatagctc gaaaagaccg atgcgctctg ctttagataa aactaagatt tcacaaacaa
     4201 catggttatc ttcaactggt ttcactggtg atgaacaggc ttataaagat catggtggac
     4261 cacataaagc agtttgtggg tttagtaagc ataattatgc actgtatcaa gatgatttac
     4321 ctacactacc tactcatgcg atgtttggag agaatttaac atttgattat ttagacgaat
     4381 ctgatgttta ctttggtaat caatatcgtt taggtgaagc gttaattgag gtttctgaaa
     4441 ttagagaacc atgctggaaa attcaagcaa aatataatat tcctgattta gtgaagcgca
     4501 tgtctacatc tggtaaaaca ggtttctatt tccgggtatt aaaacaaggc tatgtatctc
     4561 caaatgatca gctttactta atacaagaag caccaatcga acatcgttta tctgtacaac
     4621 agcttaatga cctttattat aatgatagac aaaatcaaga tatgttacga tatgcactaa
     4681 acaatccatt tctgtcacca acaagacgcg ataaacttca aaaaatgtat aacagaacat
     4741 tgaaataatt acctttcatt ataagtgtta aatgaacttt tcaaaacaaa aaggaatcaa
     4801 cttacacatc gtttgtataa tagtcttatc tataaccact gatgctattg attccttttt
     4861 tatgttatat attttcttta tttcaaactg tatattaatg atgtcacttc atttgatacg
     4921 attcttgata acctattcaa aattccgcca aataacataa atattatata aatgccgata
     4981 cttttaatca ttttctactt tttcttcgat acggaaactt gttttcgaat tgaacacttc
     5041 accagctttt aaaattgacg gtgctttttc accatataaa ttaatatcat ttggtaaaaa
     5101 ttgtgtttct aatgtaaagc cagaatgtgg tttataaata ttaaatggac tatcccactc
     5161 atcaggctgg ttaaaagtaa agaacacaac atgaggcata tctgtatcga cctctaacat
     5221 aaattcatga ttttcaacat acattttatg ttcaccaact gtaaatgggt gatcgagacc
     5281 accaaaacgt gtcatttgtt ggcttaattg cgcatgttgt gatgtaaaaa tatcactcaa
     5341 ttttatcttg ttatcttcaa acacttcatg taaatctaga atatcacctg taacaatatt
     5401 tcgctcatct aatacataca tatctaattg attacttgaa atgcgatgat tatcaacgac
     5461 attattatct cgattcaaat tgaagtacac atgattcgta ggactaaaca atgtgtcttc
     5521 tgatgcaact gcttcgtatt caatcgacca ttggtgatcc gcatcataaa tatgtgtaat
     5581 cgtcacatcg atatcacccg ggaaatgatc atcagctgat ttcaacaccg tcttaaatat
     5641 aactttaatt tgagcaattt catttctaat ttcataatca aataacttat tgtccaaacc
     5701 atgacatcca ccatgtaaat gatgttcacc gttgtttttt tctaactgat attctttacc
     5761 tttcaactta aatttagcat tatcaattct accgctatat cttcctatag aagcaccaaa
     5821 tttaaaagga ttactatgat aaaattcatc cgcttcaaca acatttccaa gaacaatatt
     5881 attatcatga tatttccaag acactactct tgctccataa ttcgtaaaaa taattttagt
     5941 ttcatcatta tcaattttga ttaaatctac accttgtctt tggtgctcaa cttcaactat
     6001 catttttact tctcccttct aaccacaagt gttcaagctc tgctgggtag caacattact
     6061 aaaacaccta caatacaaat gattgcaccg ataacatcat atttatctgg catttgttta
     6121 tctacgacca tcgcaaaaat caaactcatg atgataaata cgccaccata tgctgcatat
     6181 actcttccga atgatggaaa tgattgaaat gtcgcaatga caccatataa catgagtatc
     6241 gcaccgccta ttagcccaac aagtgaagac tgtccttccc taagccacag ccaaatcagg
     6301 tatcccccac ctatttcaca taagccagct aatataaata taaaaatcgg atataacatg
     6361 aaatcactcc atcacacatt tgctatcaat aatctatcgg ctacatatca tttgtttaca
     6421 tttcttctta cttcacattc ccattttaaa aagttcgttt tcacattcat attgtacact
     6481 tttttagaca ttattctata gctaaatata aaaaaataag agtaacacgc tttcatcatc
     6541 attttatatg ataaatgtgt gtcactctca tcaattttat tttttaaata cacgtttcat
     6601 tgaattaaat aagccacgtt caaatgtaag tactgaatct ttatatgttt taattgcaat
     6661 ccatatcaag acagctacca ttacaattga gattaaagaa cttaagatga cctcatatat
     6721 ttgaagccct gaagtttgag cgcgtacaac taattgaaat ggcgctaaaa acggaatata
     6781 acttgtgatt aaagcaagtt gtccatcagg attatttatc gtgaatatcg cgatataaaa
     6841 tgcaatcata ccaagtaatg tcagtggcat caaagattga tttaaatctt ctattctaga
     6901 tgttaatgat ccgaggatgg ctgcaagtaa tacatacgcc gtaattccaa caatactact
     6961 tataattccg acaataataa tttgccaaga caattgattc atttccacgt taaaaccttg
     7021 tagcaagtct tttaagtcaa aggcaaaaat gcatataact gccatcaata caattaaaat
     7081 aatctgagtc acggcaactg ctataacgcc taaaatttta gcaaaaatat gttggattgg
     7141 tgaaatactt gtaataatca tttcaatgac acgcgatgtt ttctcactag caatttccat
     7201 agctatttga gatgcataat ttaaaacaat gaagaacatt agaaagataa tgccataagc
     7261 taaagcatag ttgaaaatct tttgtccttc tgatacttta tcgacttcat cattagaaat
     7321 caccttatta tcaactttac tttgtgcttg taatttttgt aagtcttctt tgttgatatt
     7381 taattccccg gctaccatat ttgtttgaat agctgtaagc agtgcttgta ctttttgtga
     7441 atcttcatga cttactcgct tctcactaat gattgtccct tgtaacgtgc gattttgatt
     7501 caccttgata atataagctt tatcaagttt atgttttttt acttcttttt cagcatcttc
     7561 tatagaaact ttagtaaact tagcatcact atgaaatgta ttcgcctgtt gcttgaaaac
     7621 cttatagatt tgttcattcg gtgctgctac accaatttta tctggaccat catcaaacat
     7681 gttaataatc ttatcaatgt tagataggcc aatcattaag gcagcaataa taatcataaa
     7741 aattacaaat gatttagctt taattttttt gatatatgtc aaagtaaatg tcgcccaaaa
     7801 cttatgcatc cttgccacca accttctcaa tgaatatatc ttgtaatgat ggttctacaa
     7861 cttggaatcg tttaacataa ccttgatgtg ccacaacttg ataaatatct ttggctacgt
     7921 cttcattctc aatcgtcaac tgaagacctt gcttcatgtt ttcactatga atgatgcctc
     7981 taatgtttgt taaatctggt agtgttgttt ctgattcaat gacaactttc ttgttaccat
     8041 tagatgcacg tacatgattg atatcaccag aaacaacaag ttgaccttta tctaaaatac
     8101 aaacatcatc acataattct tcaacatgct ccatacggtg agaactataa acgattgtac
     8161 tgccccaatc atttaagtct ttaactgctt cttttaataa ctcaacatta actgggtcta
     8221 gaccactgaa aggctcatct aatattagta attctggttt atgtaacata cttgctaaca
     8281 gctgaatttt ttgttgattc ccttttgata gactatcaat tcgttttttg cggttttcag
     8341 taatatcaaa acgctcaagc caatacgata tttgctgttg tatttctgtt tttgacattc
     8401 cctttaaagt tgccaaatat ttcaattctt cttcaactgt caatttccca tgtaaaccgc
     8461 gttcttccgg taaataacca atacgattgt acattgtttt atctagtttt ttaccgttat
     8521 acgtaatgtg tccttcagtt ggttcactta agcctaaaat catacgaaat gtcgttgttt
     8581 tacctgcacc atttcttcct agaaaaccta acattttacc tgattctaac tttaatgaaa
     8641 tatcatttac tgccgtcatc ttgccaaaac gtttcgtaac atgttcaatt acaagtccca
     8701 tactttgcct cctaaaaaaa tatgtattta tcttaatata acatttccat tctctataaa
     8761 tgcaatattt ttaaaatgaa tttattttta aaatttctga aattgaaaaa tttaaatagt
     8821 gccatttttg catgttaagt atcattagca ctagatatgt tttttccatg cctttattgc
     8881 cttatttgta aaaaaatatc ccagcatcat tgtgccgaga tatttatatc attacaattg
     8941 attatctgtt tcttccggtg ttttcggtgt ttcagatgta cacgcttttt ccggtgtttc
     9001 agatgtcact ggttgatttt taattgtaga cgggtatttt gggctttcgc catatttatt
     9061 tgccggctta ctgtcaaagc ataggaatac tatcataaca attgttaggc ctaaataaac
     9121 aaaataaaga agtactaaca aaatattaag acccatcggc attaatgtaa aatcactgtc
     9181 ataataacta tcgataatct gtaatactat ataaaatata atactgaata ctgtcataat
     9241 cattggaaat aacattgttc ttgatatatc gtgaaatctt cgaacgcaca acgctaaatt
     9301 tggaataaac gttgccaaac tatagacaaa agtatacaca gatgtaagga taatcatcaa
     9361 tatactcata actattaatg tttcgttatc cgccgctata gaaataaaga atagaaatag
     9421 gtttattatt agcacacaca cagctggaac cataagtatc aaatgccata gtgccatata
     9481 ccaatattca ctacgtcttg atctcccctt aaaatttaca taatttttcc aaaataaaac
     9541 gaatgatttc ataaaaccta cttgaggtaa ttgttccatt gtaatctccc tttcgttaat
     9601 catatttata tttttaatta ttgttaccgt tataatttac aagattcatt atcaagccac
     9661 ttcatttcaa tcgtcaatca ctttggaaaa tgaactttat atttcatcac tagtaaaatg
     9721 aaaacccgct acaagtacac atctatatgg agactcattt gaaagtcaac gcttcgttaa
     9781 ctatactaaa aatatgtcat actgcaatgt tcacgtttaa aagagtctca atctatgcaa
     9841 ataaaatatt ccataacaaa gtatatactt tacattttta taattcttaa caatactatt
     9901 ttatcaaaca tttaccacaa taaaaatatc tttttcattt ttatttaaat taatcatata
     9961 attgcgagga gaatattatg gatttcgtta ataatgatac aagacaaatt gctaaaaact
    10021 tattaggtgt caaagtgatt tatcaggata ccactcaaac gtatacaggc tacatcgtgg
    10081 aaacggaagc ttacttaggt ttgaatgatc gtgcggctca tggctatggc ggtaaaataa
    10141 cacctaaagt cacgtcatta tataaacgtg gtggtacaat ttatgcacat gtcatgcata
    10201 cgcatttact cattaatttt gtaacaaaat ctgaaggtat acctgaaggc gtacttatcc
    10261 gcgcaattga accagaagaa ggtttatccg ctatgttccg taacagaggt aagaaaggct
    10321 acgaggtaac gaatggccca ggaaaatgga ctaaggcatt taacattcca cgggctatcg
    10381 atggcgctac gttaaatgac tgtagattgt ctattgatac taagaatcgt aaatatccta
    10441 aagatattat tgctagtcca cgaatcggta ttccaaataa aggtgattgg acacataaat
    10501 ctttacgtta cacagtgaaa ggtaatccat ttgtgtctcg catgcgtaaa tcagattgta
    10561 tgtttcccga agatacttgg aaataaatgc catctttcat tgattactat catgaaaatg
    10621 aaatctatct ccttataagt caatcaatcg tgccgtcaac atgcggatgg gttgattgtt
    10681 tttctttgta tccatcatat tttttgattc atctcctctt attgaacttg ttcttaatta
    10741 taaaatataa caatagaatt atttataatt attaaattta gatgcattaa tattattgat
    10801 attattttca aaaactagaa atattgattt gttgcatgta taatgttaaa agcgcccttt
    10861 tataacgctt acatataaaa gcttatttag ggagagggat attcaacaag ggggatttga
    10921 aaatgataga acttaatgca attacaacat tatgtttagc ttgtatcctt tatttacttg
    10981 gtaaggctat cgttaatcac gttaattttt taaaacgtat ttgtatacca gcaccagtga
    11041 ttggcggctt aatctttgct attttagttg cggctttgga ttcatttggc atggttaaga
    11101 ttaaattaga tgcttcattc attcaagatt tcttcatgtt agcattcttt acgacaatcg
    11161 gtcttggtgc atcattgaaa ttatttaaat taggtggcaa agtcttgcta ttatacttta
    11221 tgttttgtgc tatcatttca gtcattcaaa acatagttgg tgtatcacta gcaaaagtat
    11281 taaatattaa acctttgtta ggattaacag caggttccat gtctatggaa ggcggtcatg
    11341 gtaatgctgc tgcttatggt aagacaattc aagatttagg tattgattcg gcactgacag
    11401 cggctcttgc agctgcaact ttaggtcttg tatttggagg gcttatcggt ggtccagttg
    11461 taaaattttt aatcaaacgt tataacttaa agcctcaaca tagtgacgat acatttaaag
    11521 attatagcca agtagcatat aacgaacatt tacatagtaa atttaatgcc actgaagtat
    11581 tcttcattca atttacaatc gttgtattct gtatggcagt tggaagttat ttcagtcatt
    11641 tgtttacagc tcaaacaggg attaatgttc caatttacgt tggctcatta tttgtagctg
    11701 ttattgtccg aaatatctct gaaagtttta attttaatat tgtagattta aaaattacta
    11761 atcaaattgg cgatgtcgca ttaggtattt tcttatctct tgcgctaatg agcattcaat
    11821 taatcgaaat ttataaactt gctatacctc ttattattat cgttttagtt caagttgtcg
    11881 ttatgatttt atttgctgtt ttaattttat ttagaggttt aggaaaagat tatgatgctg
    11941 cagtaatggt aggtggtttt atcggtcatg ggcttggtgc aacgccaaat gccatggcaa
    12001 atttagatgt tattactaaa aaatatggaa actcacctaa agcatattta gttgtaccta
    12061 ttgttggtgc attcttaatc gatttaattg gtgttatagt cattatggga ttcatacaat
    12121 ggtttagtta aacaccaaac tcataaataa aagaggaggc cttcgcctcc tcttttattt
    12181 atcctcgatg tatattcaag ttacgttgtt ctatccatga caatatttcc ggactaaata
    12241 cgatttgttt ttgtgttaag tcgtcaatat ttttagcatc taacatcgtc attattgatt
    12301 tcatgtgttc aataaatgat tctacataag ctactgtatg tgcaatgcca ttattttcaa
    12361 cttgatttaa aaacggacgt gacataccag ttgcctttgc accaagtgct aaacttttaa
    12421 ttgcatcgag tggtgtacgt aaaccaccac tcgcgaaaac tgaaatttcg ctttgataag
    12481 ccgttgtttc aagtaatgac tcaactgtag actgtcccca tgatgataag taatccatat
    12541 ctttatttgc acgacgttca ttttcaatat ctacaaagtt agtaccacct ttgccactaa
    12601 catcgacata cttgacgcct atttgttgta agtcatgcat taattctttg ctcataccaa
    12661 atccaacttc ttttataatg actggaacag acactcgtga tacaatcgac gctatattat
    12721 ctaaccaagt cacaaattca cgattccctt caggcataac taattcttga ggagaattaa
    12781 catggatttg taacgcttgt gcctcaagta attcaactgc ttccaaagcc ttttctactg
    12841 gtacgtccgc accaacattg ctaaaaatca tgccttcagg attcattttt cgcgcaatcg
    12901 taaacgtctc agccatgcgt ggatttctca atgccgcatg tgttgatcca actgccatcg
    12961 ctaagccagt ttctcttgca actacagcta gcttttcatt gatgtttttc gtccactcgc
    13021 taccacccgt cattgcatta atataaaccg gatatgccat cgttaagtca ggcgtctgtg
    13081 atgtcaaatc gatatcattt acattaattg atgggataga atgatgcaca aaacgcatct
    13141 tatcaaaatc tgaatgcatt gcgtcagatt gggccattgc tatttcaaca tgttcatttt
    13201 ttctctgttc tctttgaaaa tcactcatga ttaaacctac cttttcgtca tttctaattt
    13261 aaaaccattt tttacgtctg aaaaagatga ttaatacgac agctataaca aacattacag
    13321 ctaagcaaat ataatatcca taatgtaaat gtaatgccgg catatttaca aagttcatac
    13381 cataaatccc agctatgaat gttaacggtg aaaatataac tgatactaat gtcagtactt
    13441 gcataatact attcattcta aatgacgtgt atgactcaaa attttctcgt atttcgtttg
    13501 tcatttcttg agcagtacga atgatattac gttgcttaat caagtggtca tcgatatgtt
    13561 gaatgtatag cgaatgttta ttatctataa tcaaatcacc attttgtttc attgtatcaa
    13621 ttagctcttg cataggaaac agtacacgtt ttactttaat caaatccgaa cgtaacttaa
    13681 agacactatc catgaccatt ttattaaagc gatcatctac atggcggtct tcaaaatgat
    13741 aaacactatc ttcaagtgca tatacaaagt tgaaatattt atcaaccatc atatctaaaa
    13801 ttaatatgac gacatctgca caatctaatt ctgcatctaa tgtattcata tacttataga
    13861 ctactttatt taatgattcc aacgtttgat gatgatatgt tactaataca ttgtcttgta
    13921 taaaaatatt tagtgctatt ggtgaatagt ttgaccccat aatactatgg aatactaagt
    13981 attgataatc tttataagat ttatatttag ctcgtggcat accgttaatt gcatcatcca
    14041 cttctaaatc attaaaatta aaatgtgctt taaaccattc attttcttgt tcattcggtt
    14101 catcaaaatc ataccaaaca atagtcgcat cttttggtat ctctttgata tcatcaacta
    14161 ctttaaacgg ttcatatgta gtttgatacc gtatctttaa agccatcgat actcccccta
    14221 aataacgaat tctctattat tttatcatga attaaataac gtgtatgtct taatttattt
    14281 tagtatgata gtcactaagg agatggttat tatcaaacaa ctttttacac atactcaaac
    14341 cgtaacatct gaattcattg accataacaa tcatatgcat gatgcaaatt ataatatcat
    14401 ttttagtgac gtcgtgaatc gttttaatta cagccacggt ctttctttaa aagaacgcga
    14461 aaatttagca tatacgctat ttacactaga agaacatacg acatacctct cagaattgtc
    14521 tcttggcgat gtatttactg ttactttata tatttatgat tacgattata agcggttgca
    14581 tttattttta acattaacta aagaagatgg tacactagca tcaacaaatg aagtaatgat
    14641 gatgggaatt aatcagcaca cacgtcgttc tgatgctttt cctgaatcat tttcaacaca
    14701 aatagcacac tattataaaa atcaatcaac tatcacttgg cctgaacaat taggacataa
    14761 aatagcaatt ccacacaaag gagcattaaa atgacagatg cattacaaca aaagattcat
    14821 atcgaattac tagatttatt agatgatgtt aagtttgaat taacagaatt aaatgcacaa
    14881 aaagggttat acattaacgg accagcaaat cagctactta agcgtggcgt gcatatggct
    14941 tatgttcaag gacaaaagca agccatcgat aatattatga ctattgtgga acaacagctt
    15001 gaagatcaac atttcctaga acattatgat aaatttcaaa atgaggttgc tcatcgcaat
    15061 tatgataaaa cagctaactt tgcagaactt agtgatattc cacgtcaatt cgataatttt
    15121 ttagatcaat tttatcaaat taaagggcaa tactttatca tcacacatat caatacactt
    15181 attggtgatt ttcactcaga agctcattaa caattagtct atataaccct tgctatattt
    15241 tcaaaaacaa aacccaatta cgttttcatg tcaaatatca tcttgcatga aatcgtaact
    15301 gggtcattta tatgttatta gttattttgt gttacatcct catctatcga tttggcaatt
    15361 tgtttaatag ctttatgtga ttgtctaatt ggataaattg gaaaatcatg taccatctta
    15421 ggataatcat aaaactcaat gtattgatga tgttgcaaca tcatttgttc aaatagcttc
    15481 atatcaggat gtgtcatttc acgtccacca ccaaacatat aaactggtgg caatccttct
    15541 attgtgccat taattggcga tatgcgctta tctgttaatg gtaggccatt cgcccatttt
    15601 ttcataatct cattgacacc aaactgactt agaaccgcat cttgttcgat taaggcgtcc
    15661 gaaatatctt tattagatag tgttgcatct aaaattggtg agattaaata caatttattc
    15721 ggtaatggct gttgattatc taaaagagat tgtacaaagg ataatgccag tgcaccacct
    15781 gaaccatcac ccatgactac gacattttga tgtcctactt cagatactaa ttgatcataa
    15841 acacgttgta tcgcttggaa agtatcgtca atatgaaact ctggtgtctt tggatagata
    15901 ggcagtacaa cctcatataa tgtacttaaa gtgattttat ccaacaatct ccaatggaac
    15961 ggtgatggtt gtagtgcatt gaatccaccg tgaatatata aaattttctt atcaatttga
    16021 tgtctgaaat taaagcgaaa gacttgcata tcatctaatg acaatttttc taaatttgct
    16081 ttaacattta atgttgaagg ctgcttatgt ttttttctat tttcaatttc tcttttataa
    16141 aaaaatcttt caacatcttg atcattttta aacataatcg agcgattgtg aagcaaatat
    16201 ttattgacaa cgctattcat aacacggttt ctaatcaatg tcttaaccta cctttatata
    16261 ttttatgtat ccaatgattg tctatcccct acattctttg ccaaaaaaag tatataatgt
    16321 agaagatatt ttctttttca ctttcaaatt taagactaca attgaacagt gatttttcat
    16381 cattataaca gacaactaga catattgata agtaaagaaa agaactttat acggaggtac
    16441 cttgcatgac aaatccaaat caacgattag aaccatttga tgagacattt caacaaccga
    16501 atattcatcg tggtaagcga tatggtaaga aaaaacgttc attggtaagc atgattattc
    16561 aaatcattgt tgttatatta accgcaatcg ctggatacag catgtggaaa caacccattt
    16621 ttaatattgt cttcaccaaa gaatctgtag attttgatgg cattaaaaat tttaacgaga
    16681 caattggaaa aatgcatcaa ttaaatatta atttgggtaa tatcaatgat ttacaacaag
    16741 gaatcgatcg cttaatttta gtctttaacg gtttctttat tttatgtatc gttagcctag
    16801 tcataacaat actgacaatt gtatttaatc gcacagttct aaaagtagta aatattttag
    16861 tgttaacaat tatgttagtc attacattgt atttcagtta tattattaaa gcactcgcac
    16921 aagaaattgc agatgttttc aaaaaatatt atttaaatgt accaccagat caagtgatta
    16981 cagaagctga cgctattcat aacggcatca ttttacttgg ctgtagcatc ggtttacttg
    17041 ttataagctt attcttccgt aatcgcatgc ctcgtttaaa ataatgttat atagtaggga
    17101 ttgggacaga aatgttattt tcacaaaatt catttcgttg acccacttcc tgctttttct
    17161 ttattaatga cctactacgt caatgccaaa ttttaatata attcctctaa cattctataa
    17221 atcaacaaat aaaaaaagta tcgataacca tatttggcac cgatacttta aaaatcatgt
    17281 ccccacaaca taatttttga ttagtcacac ttcaattaat ttattttatt tattcatgat
    17341 tgatactatt atctgcatct aacttttgag ctgtagcttt tgctttttta ttactttgta
    17401 aaaataaaca taaaattaag gcgatgatgc taaatatcgt tgcaactata aatgcatcat
    17461 taataccttc aaccgttgct agtttattta caaattgtag taacactttc attgcgcctt
    17521 cttgtccgcc atattgtgat gctaattcat gcatatgatc ttgtacaaca ggattcgttt
    17581 tatctaactc ttccccaaaa gctgataagt gttgtgtagt ttgtgttgtc attacagtaa
    17641 ctaagattgc tgtacctata gagcctgcta attgacgcat cgtatttaag aaagcattac
    17701 catgagaggc aagtcgtccc ggtaacgcat taatagctgc agttaccatt ggcatcatta
    17761 taaatgccat accaaatgaa cgaagtacat agatacccat gattgtcata tatggtgtat
    17821 ccatatttaa tttagttaat tcccatgttg cataagtcat tacagcaata ccaaagatag
    17881 ctaatggttt taaaccaata gtatctaaca atttacctgc aaatggtcct agtagaccca
    17941 taattagaga accaggtaat aataacaatc cggaatctaa tgctgagaat ccgcgtaaat
    18001 tttgtaaata aatcggtaat aaaatcatac caccatataa acttaacatt acaaccatat
    18061 taataattgt tgttaatgta aatgttggga atttcaatac ttctaaattc aacattggtg
    18121 atttcattct taattctcta ataacgaata gaataataaa gataatacca atcgcaaaca
    18181 ttgtttctat ctctactgaa ccccaacctt tgttgccagc ttctgagaaa ccatataaca
    18241 aagcaccaaa accaatcgta ctaaaaatga tacctgggat atcagcttta gggtttgttg
    18301 tatattgata taacttaaac catacaaaac caattaaaat agcgataatc ccgataatga
    18361 acataccgta aaacatcaca ttccaatggt aattttgtac aatataacct gataatgttg
    18421 gaccaattgc aggtgctaaa atcattgcga tacccattgt acccatggca gcaccacgtt
    18481 tttcaggtgg ataaattgta ataataacaa ttgaacctaa tggcattagt acacctgcac
    18541 caatggcttg taatacacgt ccaaccatca tgattgggaa attcattgaa atcgcacaga
    18601 ttaatgaacc aattgtaaag agtactaacg caactaaaaa taattttcga tatgaatatt
    18661 tattaaatag atacgccgta attggtatta aaataccgtt tactaacatg aatcccgtca
    18721 tcaaccattg ccctgttgac gcagaaatat taaattccgt attaattttt ggtaaagcaa
    18781 catttaataa tgtttggttt aaaatcgcaa taaacatacc gaataataat gccgctaata
    18841 ttttaccgcg tgaaacacct tcaccaaaaa taaagttttt atgttctttt tttatttttt
    18901 cattcacttt atattcttct gattcaggat ttttagcagc aactgcttcc tcatccttat
    18961 tattagtgaa tgcttcttga tctttctcag acccctttgt gtaaccattt agactaactt
    19021 ggctatgatc atcttgattg tcaacacgca actcttcatg cgtcatacgt tgtgtcgatt
    19081 gatcagttgt tttgtctaaa tcactagctt taaatttaga ttgatttgat tgacgtgtcg
    19141 taaattgttg ttccttttgt tggcgtttgc ctttttttct tgatcttatt aaaaataaat
    19201 tgataacccc aacaataatg agcgctaaaa taatgtagct aataatgaag gtcgtagtca
    19261 tttaatgacc cccttaattt ttatggattt ttacttcagc gttcattcca ggaacaactt
    19321 gtttagacgg ttctgattct agagtgattt taacaggtat tacttgagaa actttagtgt
    19381 agttaccatc actatttgat gatggcatta atgaaaagct tgcagcagtt gcttttccaa
    19441 tactatcaac tttaccttta atagaagctt tttgaccgtc aatagtcaca tcaacatctt
    19501 tacctacttc aacatcttta atatcttttt cgtcaatatt tgctgttaca tataaatcat
    19561 ctaaattgta tgcataagcg attgggttac cagcttgcac cattgaacct tccataccat
    19621 ctaatttggc aattgtacct ttttgaggca ttttaagatc catatctttc gtttcgccat
    19681 cttgaccttg tacagtaaca attgctactt tgtcaccttt atcgagtttg tcaccttgtt
    19741 taacattaag tgatttaatt tgtccagatg caggacttgc tattttaatt tgatcgccat
    19801 ttacttttgc attatcagtt gttacatagc ttgttgtttt attccaaaaa taaaagccag
    19861 caatcccaat tgctaacagt acaacaaccg taatgacatt aattaatatc atctttttca
    19921 tgaaacattt cctcctattt gcttttaaaa ttcactttat ttattataga gacatgataa
    19981 ctaaaaaaac accaacaatt tttataagag tgttggttaa aattcacaaa ggtgtaacaa
    20041 tatgaaacga caagccaaaa tagaaattca aaacgcactt gttgatttga tggctgaata
    20101 tccttttcaa gaaatatcca ctaaaatgat ttgcgcgtat tgtaatatta atcgttctac
    20161 tttttatgat tattacaaag ataaatttga tttattggat accatcaact caaagcataa
    20221 ggaaaaattc caatttcttc taagcgcgct acatcataat ttcgaaaata ttaaacagga
    20281 taagcttaaa ctttataaat tcttcattat tattgctaag tatatcaagc acaatgagca
    20341 gtttttcaaa gacattttag ttacctatcc aatgaaaaca ttattcatag actatataaa
    20401 tttagcgaga gattactatc aacaaattat gaatgactat agtactacag taaccaataa
    20461 acagctttat gttacatata tcataggcgg tcaagctggg gtgtttatta attggctttc
    20521 gaatggttgt aatgaatcac ctgaagaagt tgctgacatt ttattggcta atacgattaa
    20581 acttcaaaac taataatacg gtttcattct aataaagaaa tgctataaaa caactagata
    20641 aagttaaacc aaatggatag ataatagtac atatgtaacg gtgctatcaa tataaaaatg
    20701 ccgagatttc cacataataa tctcggcatt tttatttttg atgctaaatt cataggtcta
    20761 tgaatagctc aagttttgtt ttttaatttt tcaattcaat ataatagaag aaactatgtt
    20821 tatcatcgat ttgttttaaa taccttcata ttataaatct gtaaaataat taatataact
    20881 gctgtagcta taataataat tatgtaaggt ataggattat cttcaccttt tacccccact
    20941 aatggcgatg caacaccacc aaataaaaac tgtactaaac ctaataaact tgatgaactc
    21001 cctctaccac tactactttc atccatagct attgtaaaac caagtgtcgc aacacctgtc
    21061 actggtgcta ttaaaatgat aaatcctatc gctaaaatcc aaaagttcca atgattaagc
    21121 aatacaatcg tcactaaaat tacaccgata atctgaatca tcgtcatgcc tctcatcaat
    21181 ttttgtgagt ctataaaatc aacaaggtac ccagtaagtt gacttgatat aatgagcgta
    21241 ataccaattc ctgcaaacat ccaactaaat tgtattgcag tcatgccata aattttttga
    21301 ataataaacg gagacgcaga aatataagta aataatatta caaatgtcat accttgaatg
    21361 agcattggta atacaaaacg tggcgtcttc aataatattt tgaagttttt aaacattgtc
    21421 tttaatccac tacttgactc acgatttgtc actgttaatg attcaggtac ttttaataaa
    21481 gaacctatga ccatgacgaa gccaaagata gtcagaatga caaagaccat acgccagaca
    21541 gaataattta aaattatgcc ccctattgtt ggcgcaacaa ctggtgcaat accattaaca
    21601 agcatcaata atgccataaa cttagttagt tcattaccac tatacatatc acttgctata
    21661 gctcttgaaa taactgctgc tgcgccacct gtcactcctt gaagaaatct taatgcaacc
    21721 atcagccaaa tattatgtac aaaaacaata cctaaacttg ctaatgtaaa aataatcata
    21781 gctataataa gcggctttct gcgcccagtt gaatctgaaa ttggaccagc aaacaaatta
    21841 ccaaacgcta aaccaatcat aaacatcgat aatgttaatt gtgcgttaga cgtcgttgtt
    21901 tgaaaatcat gtctaatatc tggtaatcca ggcaaaaaca tatctatcga taatgctcca
    21961 atggccgtca aagatcctaa aataataata aatatcggtg aacgcttgtt aaaatgcgtt
    22021 aaatcattca tatgactatc tcctttatat aatgattagt ttataactat cacgaacaga
    22081 aactaaattt aaatatgctc ttttatatat tagcattagt cacaagtttc atgaacatca
    22141 ttacaactta taaaaggtat aaaatgatat aaacctcagc acttcataaa tttttccaca
    22201 aaaaacaatc ccttcaaagt aattcacact aattgtaaat actttgaaag gatgttgttt
    22261 gtattccatg acacatgcat cttatttaag ttttatactc tatttttctg atgtcttgat
    22321 taattgcaag cggtcttgcg agccttgttc aagctcatat tctttatcta cttgcttacc
    22381 gttttcattt attaatctta cggttgcggt aatcttatca ccatgttttt cagcatctat
    22441 aatttgtggt gaactaatca tcattaaact tcctttagat acacgcttct ttacatcatc
    22501 ataaaaagat gatccttttt ttatatatga tgatacaaaa tcaaaatccg actgattaaa
    22561 cgcagcatta ttcgctaaag aatatccagc aaagaattct atcaatttgt tcttcaagct
    22621 gttttcttct ttttctttct tttcaacata gtcttcgata tcttcactgt caaaattcaa
    22681 agttatctct gtattgtatt ttaaatcgct agctttaatc gtcttcgtct gtgttgtaaa
    22741 tgttttatct ttcgctttac ctgaagctga aatggtaata tctttatttt gtggatatgg
    22801 accatacgtt ttggaacttg aaaatgccat ttcatggtca tttatagtta cttttttaga
    22861 actatcattt aattttgtat cgccttttaa agttacagat atatttgctt cttcaaaatc
    22921 ttcagtaaca tctaccgttt cagaattact ttgtctaaaa tcaaatttta aatggcctgc
    22981 aaaatcaccg ttctcagttg atttcatagc tggaattcta tatgtccccg gtataaaatt
    23041 acctattggc gtcactttat ttgcttcagc tataaccatc tttttcttac caccagattt
    23101 aaactcatat tttgtttttt ctttcggttt aacaattggt tgcttggtag gtgcagtaaa
    23161 gctcatatta tcgaaaaaga tataacgtgt gccattttta cttacacgta atatattttg
    23221 accagatctg gtttgaatat aagaagctac gctggtctta ctcttattca atttatgtac
    23281 cgtattttta aggtcgctga caaattgctt aagcccaact tcatctttga tatagttaat
    23341 gtatactttt gcttcttcag aatctacttt attatcttta gtacttaata atgttgctac
    23401 tttttgttta tcattattct caatcgcatt cactaatatt ttagtttgcg cttcaggtga
    23461 attgaagttt cttagtaaaa agaacaatat aataattaag attaaaatga aaaaaccaat
    23521 tgcccaaggc accatttttc tcatcttgat gttattagtt tgtatatctt catcggttga
    23581 ctttctatat aatgcttgac gactatcaaa tttatgtcca cattgtgtac atatctgtac
    23641 atcatcttgt gcttgttgtc cgcacttcgg gcaagatttc atactttcac ctcaattctg
    23701 ttcctcttca attgttatat gtatatcaca aaaattattt cattatttta ccattattat
    23761 tttacaaata ttatcccaag aatacaattc aagaaatgat taactttact ttagaattgt
    23821 accttattac attatttatg aaagtactct ctcaattata actaaaaaga aggtgttcaa
    23881 tacatacata tttagtctta tatttgaaca ccttcaaaaa cctatttatg attgctatat
    23941 gttttaatgc gatgattgat gacttctaat acttgcctta cattttcaat atcttcagaa
    24001 tttaaatcat tagtaatatc ttgcgcaata tctgtcataa tcgcattacg ttctttcaaa
    24061 tatgctctac ctttgtcagt taaggttatg aatttcaaac gttgatcaat atttaaattt
    24121 ggtttatcta acttaactaa tttagcatcg attaactttt taattcgtcg gcttactgcg
    24181 gccttattta caccttgtct ttgcgtgatt tcactaattg tcaaaggttc tttatttaac
    24241 atacctaata cgttagactg ctctaatgaa atttcatatt cattttgtaa atctttcaac
    24301 atttttgcag ttaatgcgtt aacgttattt ataaactgct ctaaaaattg aatatggtct
    24361 tgtaaatgtt ttaccataat tatttctccc taattactat ttttataaag tatcctcagc
    24421 aatcattgta tcgcacatct aaaaaacaca aaaatattga cccatatata atataacaaa
    24481 ttaagattta catgttgctg ctttctcctt gctttaataa attaaatcat ctacatttat
    24541 atttaatatt tattataagc gaggtgtcag tatgttattg aatttattat taatatttat
    24601 acttatcgtt atttgtccgt taattttcaa ctatactctg acgttaaatt taacatgcta
    24661 catcttagtt gtcatatcga tatttttgtc actcatctat agcatcatga cctatacttt
    24721 cccttggtct atgtttgtaa catttataat aatcagtatt atcatgctat tcaaacatag
    24781 atatttattt tctcatactt ctagtcgctt atttattaaa aaattattat ctttttcata
    24841 taaatttgta ctctacacat cagcacttct atttataagt acgattcagt cacctattgt
    24901 gaaaggtttg tctatgtggt tagctatatt atgtctttcc ctgttactaa cctttatttg
    24961 ttatctcgtt tggacagtat catatagaca tagatcatac aacaaagaag ttgatatcat
    25021 aattgtgtta ggtgctggta tttttaccga atttgtcaca ccaatgcttg cagcgcgttt
    25081 agatcgtgca cttgatatct atcaacaaca agcatcagca acaaagatca ttgtatctgg
    25141 cggacaagga ccagacgaac caatttctga agctttagct atgcaacgtt atttgattgc
    25201 acatggtgtt gataaaacag atatactaat ggaatctcac tctataaata catacacaaa
    25261 ctttttatat tctaaacaaa tcatcaacaa tttgtatcat gaagcagtta aaattgtttg
    25321 tgtaacaagt cagtttcatg tattgcgcgc cttacgacta gcccaaaaat taggtttatc
    25381 tatcgatggt gtcggaagtc atacaccata tcactttttc ttacatgtgc tcatcaaaga
    25441 ttatctaggc gtaatgtatc agtatagatt gttattaacg ttatattgtt caagtagttg
    25501 gtttatatgt ctttacgccg cttttattta taatagttaa tccacaaaca aactattatt
    25561 ataggttgtt tctaatattg cgtcaaacta tctttaaata ttcgttaatc cttgattaga
    25621 caattgttat attgaaaata aataaactaa acatactaag attagctatg aagaaatcta
    25681 tgacgat
//
LOCUS       DALAAR010000257         7841 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.105, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000257 DALAAR010000000
VERSION     DALAAR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.105"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            complement(90..794)
                     /locus_tag="O2458_003022"
     CDS             complement(90..794)
                     /locus_tag="O2458_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCZ3888801.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1039..1233
                     /locus_tag="O2458_003023"
     CDS             1039..1233
                     /locus_tag="O2458_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCZ3888802.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1245..1496
                     /locus_tag="O2458_003024"
     CDS             1245..1496
                     /locus_tag="O2458_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCZ3888803.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1534..2658
                     /locus_tag="O2458_003025"
     CDS             1534..2658
                     /locus_tag="O2458_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCZ3888804.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2674..3111
                     /locus_tag="O2458_003026"
     CDS             2674..3111
                     /locus_tag="O2458_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ3888805.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            3535..4491
                     /locus_tag="O2458_003027"
     CDS             3535..4491
                     /locus_tag="O2458_003027"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ3888806.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            4691..5170
                     /locus_tag="O2458_003028"
     CDS             4691..5170
                     /locus_tag="O2458_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ3888807.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5185..6024
                     /gene="fakB2"
                     /locus_tag="O2458_003029"
     CDS             5185..6024
                     /gene="fakB2"
                     /locus_tag="O2458_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ3888808.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            6110..6643
                     /gene="msrA"
                     /locus_tag="O2458_003030"
     CDS             6110..6643
                     /gene="msrA"
                     /locus_tag="O2458_003030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ3888809.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            6636..7064
                     /gene="msrB"
                     /locus_tag="O2458_003031"
     CDS             6636..7064
                     /gene="msrB"
                     /locus_tag="O2458_003031"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ3888810.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            7076..7576
                     /locus_tag="O2458_003032"
     CDS             7076..7576
                     /locus_tag="O2458_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ3888811.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            7576..7797
                     /locus_tag="O2458_003033"
     CDS             7576..7797
                     /locus_tag="O2458_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ3888812.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         2876 a         1164 c         1394 g         2407 t
ORIGIN      
        1 aagttggggt gggacgacga aataaatttt gcgaaaatat tatttctgtc ccactccctt
       61 aaaacttatt cttttgtgta gtaagtgcgt taatagcctt gatctaactt atcaatctta
      121 cctttacgat aaaatgattt agcaatatat ccaaatggta cattgaaaac tgttgaagct
      181 aattttaata cgtacgttgt aataaatatt tcaaatacaa ctgtaccagg taaacttccg
      241 ataaatgcga tagctacaaa taaagctgta tcaattattg agcttaaaaa tgtactacca
      301 tatgcacgga tgaaaaacgt tttatcagaa ctaaatactt ttttaattag tgaaaagata
      361 aatacatcaa tatgttgacc aataatatat gcgacgattg agcctaaagc aatgcgtggc
      421 acaacatcaa agattgcgtg taatgctttt tgtgccatat cttctggtgc aggaataaaa
      481 tgtaatgaca attgcataac aataatcata attaatgttg atgaaaagcc taaccaaact
      541 gctctttttg caactctacg cccataaata tcgtttaata tatctgttgc taaataaata
      601 gaagcaaaca tgacattacc taaagttgct gaaataccaa agatttctac agttttaatc
      661 acttgtatgt tggcaatgat tgtgccaatt gcaacccatg caattaaacc ttgtttacca
      721 aaaaagcgat acataagtac cataagcacg aacgttgcaa taaacgtaac tagtcctaaa
      781 atttcattat acatattaaa atgtcctcct aaattttgat catgcgggtg tttagaaacc
      841 gctcaataaa taactaactc attttactga aaaatcgttt gcgatgcaat agcatttgaa
      901 tataaataca ttttacgtat agaattgtaa aaggtttcat tcaaatctta gggtcaaaaa
      961 tgttataata tttttatgtc aaatttaaaa cagtaacact tatttacaag gttgcaatat
     1021 tttgaagtaa taaaggaagt gtcgcgtatt ttaacttttt cagagcaaaa tgcactcgcg
     1081 aaaatagatg atttaatgaa tacttattgc aatcaatgtc caatcaaaac tcgtctgcgt
     1141 aaattagagg ggaaaacgaa ggcgcatcat ttttgtatca atgagtgttc aatagggaaa
     1201 gaaataaaac aattaggaaa tgaacttcaa taggaggaag tcaaatgaaa attatatcta
     1261 tatcagaaac accgaaccac aacacaatga agattacact tagtgaaagc agagaaggta
     1321 tgacatcaga tacgtatact aaagttgatg attcacagcc agcatttatt aatgacatct
     1381 taaaggttga aggcgttaaa tcaattttcc atgttatgga ctttatttca gtagataaag
     1441 aaaatgacgc aaattgggaa acagtattgc caaaagtaga ggctgtattc gaataaattt
     1501 ttcatcaact agtattcggg gggaataaag tatatggaaa ttttacgtat agagccaaca
     1561 ccaagtccaa atacaatgaa agttgttttg tcatatacaa gagaagacaa gttatctaat
     1621 acttataaaa aagtagaaga aacacaacca agatttataa atcagttgtt atctatagat
     1681 ggtatcactt ccatttttca tgtcatgaac ttcttagctg ttgataaggc accaaaagct
     1741 gattgggaag tcatattacc tgatattaaa gctgcttttt ctgatgcgaa taaggtttta
     1801 gaatctgtaa atgaacctca aattgacaat cattttggtg aaattaaagc tgaattatta
     1861 acttttaagg gtataccgta tcaaattaag ctaacttctg ctgaccaaga attaagagaa
     1921 caattaccac aaacatatgt tgaccatatg actcaagcgc aaacagcaca tgacaatatt
     1981 gtttttatgc gtaaatggct agatttagga aatcgctatg gaaatattca agaagtaatg
     2041 gatggtgtcc tagaagaagt gctaactacc tatccagaat cacagttacc cgtattggta
     2101 aaacatgctt tagaagaaaa tcacgcaact aataattatc atttctatcg acatgtctct
     2161 ttggatgaat atcatgcaac tgataattgg aagactcgat tacgaatgtt aaaccatttt
     2221 ccaaagccga cttttgaaga tataccgctg cttgatttag ctttatctga tgaaaaagta
     2281 ccggttagac gtcaagcgat tgtattatta ggtatgattg aaagtaaaga aattttaccg
     2341 tatttatata aggggcttcg tgataaaagt cctgttgtaa gaagaacagc aggggattgc
     2401 ataagcgatt tagggtatcc agaggcacta ccagaaatgg tgctactatt agatgatcca
     2461 cagaaaatcg ttaggtggcg tgctgctatg tttatctttg atgaaggtaa tgcagagcag
     2521 cttcccgcac taaaagccca tattaatgac aatgcgtttg aagttaaatt acaaattgaa
     2581 atggccatat ctcgtattga aaacggagat gaagcacttg gttcagtatg gaaacaaatg
     2641 gcaaacagaa caatttaatt ggaggaatta aatatgaatg catatgatgc ttatatgaaa
     2701 gaaattgcgc aacaaatgcg tggcgaatta actcaaaatg gttttacaag tttagaaacg
     2761 agcgaagcag tatcggagta tatgaaccaa gtaaatgctg atgacactac ttttgtagtt
     2821 attaactcta catgcggctg tgcagctgga ttagcaagac cagctgcagt agcagttgca
     2881 acacaaaatg aacatagacc tacaaataca gttacagttt ttgctgggca agataaagaa
     2941 gcaactgcta caatgcgaga attcattcag caagcaccat ctagtccttc gtatgcttta
     3001 ttcaaaggtc aagatttagt ttattttatg cctagagaat ttatcgaagg tagagatatt
     3061 aatgacattg caatggactt aaaggatgcc tttgacgaaa attgtaaata gtacacataa
     3121 ataaatataa aggttaacac attttataat attaaaaatg gtgtctgtca ttgaaaatag
     3181 agaatatagt tgtattctat ttgttaaata aagtccgttt ttaccaacta tattttctag
     3241 aaatttaact gttttaatag gacatcaaac ataatattca aatcatgtgt taacctcttt
     3301 tttaaaattt tttagcatta aagttataga tttgggtaaa caattaccaa ttggaaacat
     3361 atatcacgtt acgatggggt aggtacttaa tcagcatttt ataaataaag taacggaatt
     3421 catgatatta atatcatatt cctaaaatga gtgataacaa aatgctacat aaagttaagt
     3481 tatatcaaac taaatataca tactataaat aatgaaaatg aggtgttatc gcatatgttg
     3541 aattcatttg atgcagcata tcacagtctt tgtgaagaag ttttagaaat aggaaataca
     3601 cgaaatgatc gcacaaatac aggtacgatt tcgaaatttg gtcatcaact tcgctttgac
     3661 ttatctaaag gatttccact attaacgaca aagaaagttt cttttaaatt agtagcaacc
     3721 gaattattat ggttcattaa aggagataca aacatccaat acttattaaa atataataat
     3781 aatatatgga acgaatgggc ttttgaaaat tatatcaaat cagacgagta taaaggtcca
     3841 gatatgacag atttcgggca tcgtgcattg agtgatcctg aatttaacga acaatataaa
     3901 gaacaaatga aacaatttaa gcaacgtatt cttgaagatg atacatttgc gaagcaattc
     3961 ggggatttag gaaatgttta tggtaaacaa tggcgagatt gggttgataa agatggtaat
     4021 cattttgatc aacttaaaac agtaattgaa caaattaagc ataatccaga ttcaaggcga
     4081 cacatcgtat ctgcatggaa tccaacagaa attgatacaa tggcacttcc gccttgtcat
     4141 accatgttcc agttttatgt ccaagatggt aagttaagtt gccagttata ccaacgtagc
     4201 gcagatatct ttttaggtgt gccatttaat atcgcaagct acgctttatt gacacacctt
     4261 attgccaaag aatgtggact tgaagtgggt gaatttgtgc atacatttgg agatgcacat
     4321 atttattcaa atcatattga tgcgattcaa acacaattag cacgtgaaag cttcaatcct
     4381 ccaacattaa aaattaacag tgacaagtct attttcgaca taaattatga agatttggaa
     4441 attgttgact atgaatcaca tccagcaata aaagctccaa tagcagtgta gtcattgcat
     4501 agttagctaa ccatatagac atcaaaatga catcatagta ttttcaagtg caaaaaagta
     4561 cttttttgtg ttaaacgttt tcataaatta tgcaaaatca ttatttctat cacactttat
     4621 gataaaaatt gtgttaaatt aaagataact tagtaataaa aaatgaaatg atagaagaag
     4681 gaggataatt atgactttat ccattctagt tgcacatgac ttgcaacgag taattggttt
     4741 tgaaaatcaa ttaccttggc acctaccaaa tgatttgaag catgttaaaa aattatcaac
     4801 aggtcatact ttagtaatgg gtcgtaagac atttgaatcg attggtaaac cactaccgaa
     4861 tcgtcgaaat gttgtactta cttcagatac aagtttcaac gtagagggcg ttgatgtaat
     4921 tcactctatt gaagatattt accaactacc gggccatgtt ttcatatttg gagggcaaac
     4981 attatttgaa gaaatgattg ataaagtgga cgacatgtat attactgtta ttgaaggtaa
     5041 attccgtggt gatacgttct ttccacctta tacatttgaa gactgggaag ttgcctcttc
     5101 agttgaaggt aaactagatg agaaaaatac aattccacat acctttctac atttaattcg
     5161 taaaaaataa gggggaaaac gaccatgaca aaacagatta tagtaacaga ctcaacatcc
     5221 gatttatcta aagaatactt agaagcaaac aacattcatg taattccttt aagtttaact
     5281 attgaaggag cttcatacgt tgaccaagta gatattacat cagaagaatt tattaatcat
     5341 attgaaaatg atgaagatgt aaagacaagt cagccagcca taggtgaatt tatatctgct
     5401 tatgaagaac taggaaaaga tggctctgaa atcataagta ttcatctttc ttcaggatta
     5461 agtggtacat ataacactgc ttaccaagca agtcaaatgg tagatgctaa tgtaactgtt
     5521 attgattcaa aatctatttc ttttggttta gggtatcaaa tacaacacct agtagagctt
     5581 gtaaaagaag gtgtctcaac ttctgaaata gttaaaaagt taaatcattt aagagaaaac
     5641 attaaattat ttgtagttat agggcaattg aatcaattaa ttaaaggtgg cagaattagt
     5701 aaaacaaaag gtttgattgg taatcttatg aaaattaaac caattggtac actagatgat
     5761 ggtcgcttag agcttgtgca caatgcgaga actcaaaatt ctagtatcca atacttgaaa
     5821 aaggaaattg ctgaatttat aggagatcat gaaatcaaat ccattggtgt cgcacatgct
     5881 aacgtcattg aatatgttga taaattgaag aaagttttta atgaagcttt tcatgtgaat
     5941 aattacgata taaatgtaac tacaccagtt atttctgcac atactggtca aggtgcgatt
     6001 ggcctcgtag tccttaagaa gtaaatttaa tcttttcagt gttaattact tccatttcaa
     6061 tcctttatag actaaattta taattagata gatagaggag gtaattcata tgacaaaaga
     6121 atatgcaaca ttagcaggag gatgtttctg gtgcatggtt aaaccattta catcatatcc
     6181 aggcatcaag tcagtcgtat ctggttatag tggcggtcat gttgacaacc caacttatga
     6241 acaggtatgt acgaatcaaa ccggccatgt cgaagcagta caaattacgt ttgatccaga
     6301 ggttacttcc tttgaaaata tattagacat atatttcaaa acatttgacc caactgatga
     6361 tcaagggcaa tttttcgata gaggcgaaag ctatcaacca gtcattttct atcatgatga
     6421 acatcagaaa aaggctgctg agtttaaaaa gcaacaatta aatgaacaag gtattttcaa
     6481 gaaaccagtg attacaccta ttaaaccata taaaaatttc tatccagctg aagactacca
     6541 tcaagattat tacaaaaaga acccggtaca ttattaccaa tatcaacgtg gttcaggtag
     6601 aaaagcgttt atagaatcac attgggggaa tcaaaatgct taaaaaagat aaaagtgaac
     6661 taacagatat agaatatatt gttacacaag aaaacggcac tgaaccacca tttatgaatg
     6721 aatattggaa tcattttgct aaaggaattt atgtagataa aatttctggt aaacctttat
     6781 ttacatctga agaaaagttt cattctgaat gtggatggcc tagcttttcc aaagcgcttg
     6841 atgacgatga aattatagaa ttagtcgaca aatcatttgg tatgttgaga actgaagtgc
     6901 gttcagaaga atcaaatagt catttaggac atgtctttaa tgatggacct aaagaaagtg
     6961 gcggcttaag atactgtatc aattccgctg caattcaatt tattccatat gaaaagttag
     7021 aagaattggg ttatggcgat ttaatatcac attttgataa gtaggagtgg aaagtatgtt
     7081 taaaaaatta ttcggaaaag gcaaagaagt tcagaaagat atcgcaattt atgcaccatt
     7141 aactggagaa ttcgtgaaaa ttgaagatat tccagatcct gtattcgcac aaaaaatgat
     7201 gggcgaaggt tttggtatta atccaactga aggagaagtt gtgtctccaa ttgcaggacg
     7261 tgttgacaat gtctttccaa ctaagcatgc gattgggtta aaagcagata acggattaga
     7321 attattagtt catatcggtt tagacacagt tcaattagac ggtgaaggat ttgaagtgtt
     7381 agtgtctagt ggtgacgaag ttaacgtcgg agatccatta gtaagattca accttgaata
     7441 tattaataat aacgctaaat ctgtaatttc accaattata attactaaca ctgatcaagc
     7501 agcttcaatt aatatttatg atgaaaatgc tgtgattaaa ggtgaaacaa aagtgattga
     7561 tgtgacaatg aactaatgaa aaactattcg ttttatcaat ttgtcatgac agttcgtggt
     7621 cgacacgacg ataaaggtcg tctagcagaa gagatatttg acgatcttgc tttcccaaaa
     7681 cacgatgatg attttaacat actgtctgat tatattgaga cacatggtga tttcacattg
     7741 ccaatgtctg tatttgatga tttatatgaa gaatatacgg aatggctaaa attttaatat
     7801 aatttttaat aataatagtt agaaccaggg tgatgcaatt c
//
LOCUS       DALAAR010000258         6869 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.113, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000258 DALAAR010000000
VERSION     DALAAR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.113"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..83
                     /locus_tag="O2458_003034"
     CDS             <1..83
                     /locus_tag="O2458_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ3888813.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O2458_003035"
     CDS             complement(255..1226)
                     /locus_tag="O2458_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ3888814.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O2458_003036"
     CDS             1350..1769
                     /locus_tag="O2458_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ3888815.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O2458_003037"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O2458_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ3888816.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5004
                     /gene="blaR1"
                     /locus_tag="O2458_003038"
     CDS             3247..5004
                     /gene="blaR1"
                     /locus_tag="O2458_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ3888817.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4994..5374
                     /gene="blaI"
                     /locus_tag="O2458_003039"
     CDS             4994..5374
                     /gene="blaI"
                     /locus_tag="O2458_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ3888818.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5638..6216
                     /locus_tag="O2458_003040"
     CDS             5638..6216
                     /locus_tag="O2458_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888819.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(6439..6624)
                     /locus_tag="O2458_003041"
     CDS             complement(6439..6624)
                     /locus_tag="O2458_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888820.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         2574 a          950 c          945 g         2400 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatcttcac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
     3721 attgtgattc gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat
     3781 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
     3841 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
     3901 atcttctcta ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt
     3961 catgacaatg aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt
     4021 aaaacttatg cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat
     4081 atattaagcc attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata
     4141 aaagaagccg atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact
     4201 ttttttatca tgataattca aagccagttt ttaatggggc aatccttaac tgattataat
     4261 tataaaaagc ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca
     4321 aatagtggat ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa
     4381 aaagaaagta gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt
     4441 ggacttgacc gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat
     4501 tatcttttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
     4561 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     4621 aagcaattaa attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat
     4681 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     4741 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     4801 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     4861 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     4921 tcagatggaa acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga
     4981 atgggtgttt taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat
     5041 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     5101 atataaagaa gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga
     5161 gattataaaa cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga
     5221 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa
     5281 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     5341 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac
     5401 atatgtaaac catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat
     5461 tattaaagat gagagtgtcc agaaaataac cattttctgt acactcttgt tttttgtcta
     5521 aataagtaag tattacatag aattatgtgt tcaaaaaccc aacaaaaaat ctatgttcac
     5581 aaaagacctc gtaatcaagt tatggtacac taaaggtgta tataggaggt ctgtattttg
     5641 aaaataggtt atgcaagagt ttcaactgga ttacaaaatt tgaatttaca ggaggatcga
     5701 ttaaatacat atggttgtga aaagatattt aatgaccata tgagtggttc aaaaagtaag
     5761 cgacctggtt tagataaagc aattgaattt gcgaggtcag gagatacgat tgttgtttgg
     5821 agattagata gactaggacg gaacatggag gatttaatca cgttagttaa tgaacttaac
     5881 gaacgaggtg tgagttttca tagtttagaa gaaaatataa cgatggataa atcaagttct
     5941 acaggacaat tattatttca tttattcgca gcatttgcag agtttgaacg taatttaatt
     6001 ttagagcgat catcagccgg tcgaattgca gcacgtgcta gaggccgata tggaggtaga
     6061 cctgaaaaat taaataaaca agatttaaac ttacttaaga cactttatga taatggtacg
     6121 ccaattaaaa caattgcaga gcagtggcaa gtttcacgta caactattta tcgttatctc
     6181 aataaattag aggcaaaaga gaataaaaac aagtagagca actaatctag cttactataa
     6241 tgaatctact aaataatttt atatatcatt aacatacaaa gactttgatt caatgtcact
     6301 cgatgtaaaa gagacgttct gacagcgtct aaaatctcat aatattttcc tgttatgaga
     6361 ttttaataat aacacaagaa aaaagcgtac aaattcgtac gcttttttaa tataatacct
     6421 gtttcactaa tgaacgtttt aagtatcttc atcattaact gaaactacag taataatact
     6481 cgtcaacaat attagtatag ttaaaataga aagacttagg actggcataa ccgaactaaa
     6541 attatatttg aaatataaaa gtgctaatga agagagaaat gtaaaaataa taaataccaa
     6601 agaccatttt tctaatttat tcattggctt cacctctttg aacttttata taatcatagg
     6661 agatattttt actacgattt ataaaaaata agaagataat cattattaaa gaaacgatta
     6721 atataatgtt gaatcctata gtatcaccta attcgtttac acctttgcct aatatcggag
     6781 atacaaagct ttgtattaat aatgaaagag atgcccatat agtaaatgag gttctgttgc
     6841 aaagttgaat ttatagtata attttaaca
//
LOCUS       DALAAR010000259         6633 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.117, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000259 DALAAR010000000
VERSION     DALAAR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.117"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O2458_003042"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O2458_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ3888821.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O2458_003043"
     CDS             2023..2922
                     /locus_tag="O2458_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ3888822.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O2458_003044"
     CDS             3408..4016
                     /locus_tag="O2458_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ3888823.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O2458_003045"
     CDS             4262..4600
                     /locus_tag="O2458_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888824.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O2458_003046"
     CDS             4605..4790
                     /locus_tag="O2458_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ3888825.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O2458_003047"
     CDS             complement(5017..5760)
                     /locus_tag="O2458_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ3888826.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="O2458_003048"
     CDS             6186..>6633
                     /locus_tag="O2458_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCZ3888827.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DALAAR010000260         3731 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.146, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000260 DALAAR010000000
VERSION     DALAAR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.146"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            39..2048
                     /gene="mecA"
                     /locus_tag="O2458_003049"
     CDS             39..2048
                     /gene="mecA"
                     /locus_tag="O2458_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCZ3888828.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2094..2522)
                     /locus_tag="O2458_003050"
     CDS             complement(2094..2522)
                     /locus_tag="O2458_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ3888829.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2619..3362)
                     /locus_tag="O2458_003051"
     CDS             complement(2619..3362)
                     /locus_tag="O2458_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ3888830.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1343 a          590 c          569 g         1229 t
ORIGIN      
        1 gtaatatact acaaatgtag tcttatataa ggagtatatt gatgaaaaag ataaaaattg
       61 ttccacttat tttaatagtt gtagttgtcg ggtttggtat atatttttat gcttcaaaag
      121 ataaagaaat taataatact attgatgcaa ttgaagataa aaatttcaaa caagtttata
      181 aagatagcag ttatatttct aaaagcgata atggtgaagt agaaatgact gaacgtccga
      241 taaaaatata taatagttta ggcgttaaag atataaacat tcaggatcgt aaaataaaaa
      301 aagtatctaa aaataaaaaa cgagtagatg ctcaatataa aattaaaaca aactacggta
      361 acattgatcg caacgttcaa tttaattttg ttaaagaaga tggtatgtgg aagttagatt
      421 gggatcatag cgtcattatt ccaggaatgc agaaagacca aagcatacat attgaaaatt
      481 taaaatcaga acgtggtaaa attttagacc gaaacaatgt ggaattggcc aatacaggaa
      541 cagcatatga gataggcatc gttccaaaga atgtatctaa aaaagattat aaagcaatcg
      601 ctaaagaact aagtatttct gaagactata tcaaacaaca aatggatcaa aattgggtac
      661 aagatgatac cttcgttcca cttaaaaccg ttaaaaaaat ggatgaatat ttaagtgatt
      721 tcgcaaaaaa atttcatctt acaactaatg aaacagaaag tcgtaactat cctctaggaa
      781 aagcgacttc acatctatta ggttatgttg gtcccattaa ctctgaagaa ttaaaacaaa
      841 aagaatataa aggctataaa gatgatgcag ttattggtaa aaagggactc gaaaaacttt
      901 acgataaaaa gctccaacat gaagatggct atcgtgtcac aatcgttgac gataatagca
      961 atacaatcgc acatacatta atagagaaaa agaaaaaaga tggcaaagat attcaactaa
     1021 ctattgatgc taaagttcaa aagagtattt ataacaacat gaaaaatgat tatggctcag
     1081 gtactgctat ccaccctcaa acaggtgaat tattagcact tgtaagcaca ccttcatatg
     1141 acgtctatcc atttatgtat ggcatgagta acgaagaata taataaatta accgaagata
     1201 aaaaagaacc tctgctcaac aagttccaga ttacaacttc accaggttca actcaaaaaa
     1261 tattaacagc aatgattggg ttaaataaca aaacattaga cgataaaaca agttataaaa
     1321 tcgatggtaa aggttggcaa aaagataaat cttggggtgg ttacaacgtt acaagatatg
     1381 aagtggtaaa tggtaatatc gacttaaaac aagcaataga atcatcagat aacattttct
     1441 ttgctagagt agcactcgaa ttaggcagta agaaatttga aaaaggcatg aaaaaactag
     1501 gtgttggtga agatatacca agtgattatc cattttataa tgctcaaatt tcaaacaaaa
     1561 atttagataa tgaaatatta ttagctgatt caggttacgg acaaggtgaa atactgatta
     1621 acccagtaca gatcctttca atctatagcg cattagaaaa taatggcaat attaacgcac
     1681 ctcacttatt aaaagacacg aaaaacaaag tttggaagaa aaatattatt tccaaagaaa
     1741 atatcaatct attaactgat ggtatgcaac aagtcgtaaa taaaacacat aaagaagata
     1801 tttatagatc ttatgcaaac ttaattggca aatccggtac tgcagaactc aaaatgaaac
     1861 aaggagaaac tggcagacaa attgggtggt ttatatcata tgataaagat aatccaaaca
     1921 tgatgatggc tattaatgtt aaagatgtac aagataaagg aatggctagc tacaatgcca
     1981 aaatctcagg taaagtgtat gatgagctat atgagaacgg taataaaaaa tacgatatag
     2041 atgaataaca aaacagtgaa gcaatccgta acgatggttg cttcactgtt ttattatgaa
     2101 ttattaataa gtgctgttac ttctccctta aatacaattt cttcattttc attgtatgtt
     2161 gaaagtgaca ctgtaacgag tccattttct ttttttatgg atttcttatt tgtaatttca
     2221 gcgataacgt acaatgtatt acctgggtat acaggtttaa taaatttaac gttattcatt
     2281 tgtgttcctg ctacaacttc ttctccgtat ttaccttctt ctacccataa tttaaatgat
     2341 attgaaagtg tatgcatgcc agatgcaatg atacctttaa atctactttg ttctgctttt
     2401 tctttatcta tatgcatata ttgaggatca aaagttgttg caaattggat aatttcttct
     2461 tctgtaatat gaaggctttt tgttttgaat gtttctccta ctataaaatc atcgtatttc
     2521 atatatgtct ctctttctta ttcaaattaa ttttttagta tgtaacatgt taaaggtaag
     2581 tctaccgtca ctgaaacgta agactcacct ctaactttct attgagacaa atgcaccatt
     2641 ttatctgcat tgtctgtaaa gataccatca actccccaat tagcaagttg gtttgcacgt
     2701 gctggtttgt ttacagtcca tacgttcaat tcataacccg cttcttttac catttttact
     2761 tttgctttag taagtttggc atcttcagtg tttactattt tagcattaca gtaatctaaa
     2821 agtgttctcc agtcttcacg aaacgaagtt gtatggaata taactgctct gttatattgt
     2881 ggcatgattt cttctgcaag tttaacaagc acaacattaa agcttgaaat gagcacttct
     2941 tgattctgat ttaagtttgt taattgttct tccacttgct taaccatact tttagaaagt
     3001 gctagtccat tcggtccagt aatacctttt aattctacat ttaaattcat attatattca
     3061 tttgctattt ttactacatc atcgaaagtt ggcaaatgtt catctttgaa tttttcacca
     3121 aaccaagatc ctgcagaagc atctttaatt tcatcataat tcaattcagt tatttccccg
     3181 gacatatttg tagtccgttc taaataatca tcatgaatga taatcagttg ttcatctttt
     3241 gtaattgcaa catctaactc caaccagttt ataccttcta cttctgaagc agctttaaat
     3301 gatgcaattg tattttccgg agctttacta ggtaatcctc tatgtccata tacagttagc
     3361 atattacctc tccttgcatt tttatttttt taattaacgt aactgtatta tcacattaat
     3421 cgcactttta tttccattaa aaagagatga atatcataaa taaagaagtc gatagattcg
     3481 tattgattat ggagttaatc tacgtctcat ctcattttta aaaaatcatt tatgtcccaa
     3541 gctccatttt gtaatcaagt ctagtttttc tgtacccctt atctgcaatt ttacttagga
     3601 ttgcttttaa cttacccctt atcagcaatt ttactgagaa ctgcttttaa cgcacctctt
     3661 atctgcaatt ttgcctagaa ctgcttttaa cgtacctctt atctgcaatt ttactgagaa
     3721 ctgcttttaa c
//
LOCUS       DALAAR010000261         3422 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.152, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000261 DALAAR010000000
VERSION     DALAAR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.152"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            <1..104
                     /locus_tag="O2458_003052"
     CDS             <1..104
                     /locus_tag="O2458_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ3888831.1"
                     /translation="GIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            162..539
                     /locus_tag="O2458_003053"
                     /pseudo
     CDS             162..539
                     /locus_tag="O2458_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            536..1417
                     /locus_tag="O2458_003054"
                     /pseudo
     CDS             536..1417
                     /locus_tag="O2458_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1533..2462
                     /locus_tag="O2458_003055"
     CDS             1533..2462
                     /locus_tag="O2458_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ3888832.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2455..3192
                     /locus_tag="O2458_003056"
     CDS             2455..3192
                     /locus_tag="O2458_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ3888833.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3206..3409
                     /locus_tag="O2458_003057"
     CDS             3206..3409
                     /locus_tag="O2458_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ3888834.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1243 a          458 c          608 g         1113 t
ORIGIN      
        1 aaggtattga atgtatttac gctctatata aaaagaaccg taggtctctt cagatctacg
       61 gattttcccc atgccacgaa attagcatca tgctagcaag ttaaacgaac actgacatga
      121 tatattagtg attagctata ttttttactt tgcaacagaa ccagccattt aatatgactg
      181 ttacaattac taagattcaa gcactattac gccgcacgta tgatttcacc actcaagtta
      241 ataatttaac agtagatgat tgcgagttac tacttgatgc agcaaagctt aaatttaatg
      301 aagaaaccat agacctgaca cacactgaat tgcaaatcat gcatagctta ttccagaaca
      361 agggcaaatt cgtcagtcgt aatgcgttaa tcgaaaaatg ctgggaatca gaacacttca
      421 ttgatgacaa tacgcttgct gtcaacatta cacgtttacg caaaaagcta caaaaactag
      481 gacttacaga ttttatcgaa acgaagaaaa atgtcggata tcgtgtaggt gacgtatgaa
      541 tttcttgaaa tcaatccaac gtgaaattta tattgtcatc cttacttgtg ctttattcta
      601 ctttatatta ttcatgtata atgtgccgat gcaagcatat atgattggtc taggtattgt
      661 attgtttgtt tcaatgattt actggggtat ccattacatg aactttaaaa aagaataaac
      721 actacaggaa cagaacgaag atttaagaat cgaaaaccat aaacttcgta atgactttat
      781 taactaccaa agtgagttag agtcatattt tttaatgtgg gtgcatcaaa tcaagacacc
      841 gattacagca tcaaagttac tgcttgaaga tcctgatgaa cacgtgattt ctaacgtgcg
      901 taatgaagtc ttacaaattg ataattacac gaatcttgca ctgaactatt tgaaactcat
      961 gaaccaaaag acatacatgg tctttatgga agtgagtgta gatgaattaa ttcgaccact
     1021 gattaaacgt tatgcgatac agtttattca tcatcaaaca aagttgcatt atgagaaaag
     1081 tgaagcggtt atattaacag atgccaaatg ggcatcaatt atgattgagc aaatattaaa
     1141 caatgcatta aaatatgcac gtggcaaaga tgtgtggatt acgtttgacg aaaatacaaa
     1201 gaaattatca atccgagata acggtatcgg cattaatgcg agtgatttac cgaaaatctt
     1261 tgataaaggt ttctcgggct ataacggtag aataagtgag aaatcaagcg gtattggttt
     1321 atatattgtc agcacaatct caaagcgctt aggccataaa gtaagtgcta catctacatt
     1381 aaacgatggt tcaatattca ccattcaatt taaataactc caatctttca tttttgtaag
     1441 attagcggct gtttttgtaa gaatagataa aggcagcctt ttctaatcgc gcgtaagata
     1501 aaggtaacaa caaactaaga aaggtgccag aggtgaaaaa tttgaataat ataattgaaa
     1561 taaataactt aacaaagcaa tatggaagtc agatagcagt taataatttg agtataactg
     1621 ttgaaagtgg acaaatttat ggttttttag gaagaaatgg tgcaggcaaa acaactacta
     1681 taagaatgct tttaggctta attaaaccta cgaaaggtaa tattaaaata tttgaaaaag
     1741 agttcgaaag taataaaact gaaattttaa aaaatattgg tgctatggtt gaaacgccag
     1801 gtttttatag taatttgaca gctaaagaga acttaactat aaatgctaaa atattaggta
     1861 tacaaaaaga aaatgcaatt gaagaagtgt tagaaatagt aggactagct tctgaaaaag
     1921 taaaattgtt taaaaattat tctttaggta tgaagcaacg tttagggatt gcgagagctt
     1981 tattgcacaa tcccgaatta ttgatacttg atgagccgac gaatggatta gatcctattg
     2041 gcataaaaga aattagaaag ttgattaagg atctggctca atctagaaat ataaccattt
     2101 taatttcaag tcatatattg tcagaaatag agcagttagt agacaaggtg ggtattattc
     2161 ataacggagt tttgttagaa gaaattatgt atgaagattt aaaaaaaaag aacagacaat
     2221 ttataagtat atcagtatca gatgaagaaa aagttgcatg tttgttggaa actaacttcg
     2281 gaatttatga ctataaaatt aaagaaaatg gcgaatttaa aatttattca catattgata
     2341 gacaacaaga aattaataga ttattagtat taaacaatat tgatgttttc cagatgagag
     2401 tgatagaaga taatctagaa acttatttcg aagacttagt aggaggtggc acaattggat
     2461 aatctactcc gtacagaatt tattaagtta aaaagatcat atatgtttct aataagtatt
     2521 ttaggtacat gcgtagcacc ttttatggtg gttgtagctt cttttattag tatgcggacg
     2581 aaagtaccga ctcctaatat tgtatttaaa cagttatttg aagaagtcag tttatatcat
     2641 atgctaatta ttggacctcc tctatatggg gttataatag cctatttgtt tggaagagaa
     2701 tatatagaag atactttaaa aaacttatta attataccta tatctagaga aagtttttta
     2761 gtaagtaaaa taatattatt attcttgtgg attcaaacac tatctgttat tgcatggttg
     2821 ctaactttaa tgctaggtat gctattgcat tttcaagggt taagccagag tttaataatt
     2881 gattcattct ttacatttgt tattggcgga gtgttattat ggatattatc tccaccaata
     2941 attattttgt cgatatattt taaaaattat gttcccacaa ttgttttaac cataataatt
     3001 actttgataa atttaatgac tgctaattct gaacatagag gattatttcc ttgggctgca
     3061 gttggagaca tatctagagg aacattacct aatacttatc catttctatt atctttaatt
     3121 atcataattg ttacatctat attcagtgta attatattat tctcatactt taaaaaaact
     3181 gatataagtt aaaaggagga gaaaaatggg ttttatagag cagattaaag catttatttt
     3241 aggtgtagtt gaagggttga cagaatttgc tccagtatca tcaacaggtc atatgattat
     3301 tgttgatgat atgtggttaa acactgctga cgatttagga agtgcgtctg caactacttt
     3361 taaggtggta atacagttag gaggttctgt tgcaaagttg aatttataat ataattttaa
     3421 ca
//
LOCUS       DALAAR010000262         1553 bp    DNA     linear   BCT 13-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 140 from
            subject 263 SAMN14154580-rid16266693.guided.193, whole genome
            shotgun sequence.
ACCESSION   DALAAR010000262 DALAAR010000000
VERSION     DALAAR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154580
            Sequence Read Archive: SRR11139413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525675.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525675.1
            Genome Coverage       :: 294x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:00:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,944
            CDSs (with protein)               :: 2,944
            Genes (RNA)                       :: 54
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154580-rid16266693.guided.193"
                     /strain="ST8"
                     /isolate="Sample 140 from subject 263"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="Cook County Health"
     gene            91..1077
                     /locus_tag="O2458_003058"
     CDS             91..1077
                     /locus_tag="O2458_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ3888835.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1049..1309
                     /locus_tag="O2458_003059"
     CDS             1049..1309
                     /locus_tag="O2458_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ3888836.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1300..>1553)
                     /locus_tag="O2458_003060"
     CDS             complement(1300..>1553)
                     /locus_tag="O2458_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCZ3888837.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          558 a          214 c          256 g          525 t
ORIGIN      
        1 ccttatataa gactacattt gtagtatatt acaaatgtag tatttatgtc aaaataatgt
       61 tataattttt gtgatatgga ggtgtagaag gtgttatcat cttttttaat gttaagtata
      121 atcagttcat tgctcacgat atgtgtaatt tttttagtga gaatgctcta tataaaatat
      181 actcaaaata ttatgtcaca taagatttgg ttattagtgc tcgtctccac gttaattcca
      241 ttaataccat tttacaaaat atcgaatttt acattttcaa aagatatgat gaatcgaaat
      301 gtatctgaca cgacttcttc ggttagtcat atgttagatg gtcaacaatc atctgttacg
      361 aaagacttag caattaatgt taatcagttt gagacctcaa atataacgta tatgattctt
      421 ttgatatggg tatttggtag tttgttgtgc ttattttata tgattaaggc attccgacaa
      481 attgatgtta ttaaaagttc gtcattggaa tcgtcatatc ttaatgaacg acttaaagta
      541 tgtcaaagta agatgcagtt ctacaaaaag catataacaa ttagttatag ttcaaacatt
      601 gataatccga tggtatttgg tttagtgaaa tcccaaattg tactaccaac tgtcgtagtc
      661 gaaaccatga atgacaaaga aattgaatat attattctac atgaactatc acatgtgaaa
      721 agtcatgact taatattcaa ccagctttat gttgttttta aaatgatatt ctggtttaat
      781 cctgcactat atataagtaa aacaatgatg gacaatgact gtgaaaaagt atgtgataga
      841 aacgttttaa aaattttgaa tcgccatgaa catatacgtt atggtgaatc gatattaaaa
      901 tgctctattt taaaatctca gcacataaat aatgtggcag cacaatattt actaggtttt
      961 aattcaaata ttaaagaacg tgttaagtat attgcacttt atgattcaat gcctaaacct
     1021 aatcgaaaca agcgtattgt tgcgtatatt gtatgtagta tatcgagctt cacatgaaac
     1081 agctaaagaa gctttgggcg ataaagagtt aagagccatt gcacatgagt taactaaaac
     1141 agttaaggat aacatgagtg ttgattggtc taaacgagac agtgctaaag ctaaaatgag
     1201 agttcaagtt agacgcctat taaagaaata tggctatcca ccagatcttc aaaaaatggc
     1261 tgtggaacaa gttgtagagc aagcagaatt aatggcaagt cagcaataaa aaaataaatc
     1321 ataatgagtc cgggacataa agttcttgga taagtgaaaa aagacaattt ctattgaaat
     1381 aatatagaaa ttgtcttttt tataaatttt ttgattattt tcagctcgtt gagctactac
     1441 ttttcttata ttaagtgcca ttaatacaaa accaagttct cttttgactt tattgagtcc
     1501 tcggacagac atccgagtga aacccaaaat agccttcata aatccaaaaa cag
//
